LOCUS       DAIGOK010000001        56831 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.001, whole genome shotgun sequence.
ACCESSION   DAIGOK010000001 DAIGOK010000000
VERSION     DAIGOK010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56831
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.001"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            168..983
                     /gene="thiD"
                     /locus_tag="NOA01_000001"
     CDS             168..983
                     /gene="thiD"
                     /locus_tag="NOA01_000001"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCI0631242.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(1312..2763)
                     /gene="cls"
                     /locus_tag="NOA01_000002"
     CDS             complement(1312..2763)
                     /gene="cls"
                     /locus_tag="NOA01_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCI0631243.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            3104..5770
                     /locus_tag="NOA01_000003"
     CDS             3104..5770
                     /locus_tag="NOA01_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCI0631244.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            6134..6886
                     /locus_tag="NOA01_000004"
     CDS             6134..6886
                     /locus_tag="NOA01_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCI0631245.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(6908..8062)
                     /locus_tag="NOA01_000005"
     CDS             complement(6908..8062)
                     /locus_tag="NOA01_000005"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HCI0631246.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            8247..10688
                     /locus_tag="NOA01_000006"
     CDS             8247..10688
                     /locus_tag="NOA01_000006"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HCI0631247.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(11018..11722)
                     /locus_tag="NOA01_000007"
     CDS             complement(11018..11722)
                     /locus_tag="NOA01_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631248.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(11909..12154)
                     /locus_tag="NOA01_000008"
     CDS             complement(11909..12154)
                     /locus_tag="NOA01_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631249.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(12171..13022)
                     /locus_tag="NOA01_000009"
     CDS             complement(12171..13022)
                     /locus_tag="NOA01_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCI0631250.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            13188..14192
                     /locus_tag="NOA01_000010"
     CDS             13188..14192
                     /locus_tag="NOA01_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HCI0631251.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(14433..14789)
                     /locus_tag="NOA01_000011"
     CDS             complement(14433..14789)
                     /locus_tag="NOA01_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCI0631252.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            15157..16344
                     /gene="tuf"
                     /locus_tag="NOA01_000012"
     CDS             15157..16344
                     /gene="tuf"
                     /locus_tag="NOA01_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCI0631253.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(16532..19123)
                     /gene="mprF"
                     /locus_tag="NOA01_000013"
     CDS             complement(16532..19123)
                     /gene="mprF"
                     /locus_tag="NOA01_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCI0631254.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(19149..19910)
                     /locus_tag="NOA01_000014"
     CDS             complement(19149..19910)
                     /locus_tag="NOA01_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCI0631255.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            20077..20709
                     /locus_tag="NOA01_000015"
     CDS             20077..20709
                     /locus_tag="NOA01_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HCI0631256.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            20768..21628
                     /locus_tag="NOA01_000016"
     CDS             20768..21628
                     /locus_tag="NOA01_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HCI0631257.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            21813..24128
                     /locus_tag="NOA01_000017"
     CDS             21813..24128
                     /locus_tag="NOA01_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCI0631258.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            24310..26151
                     /locus_tag="NOA01_000018"
     CDS             24310..26151
                     /locus_tag="NOA01_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCI0631259.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            26175..27284
                     /gene="rpoD"
                     /locus_tag="NOA01_000019"
     CDS             26175..27284
                     /gene="rpoD"
                     /locus_tag="NOA01_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCI0631260.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            27504..29603
                     /locus_tag="NOA01_000020"
     CDS             27504..29603
                     /locus_tag="NOA01_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HCI0631261.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            29799..30662
                     /locus_tag="NOA01_000021"
     CDS             29799..30662
                     /locus_tag="NOA01_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCI0631262.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYGFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            30767..31240
                     /locus_tag="NOA01_000022"
     CDS             30767..31240
                     /locus_tag="NOA01_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCI0631263.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            31336..32223
                     /gene="xerD"
                     /locus_tag="NOA01_000023"
     CDS             31336..32223
                     /gene="xerD"
                     /locus_tag="NOA01_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCI0631264.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            32242..32613
                     /locus_tag="NOA01_000024"
     CDS             32242..32613
                     /locus_tag="NOA01_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCI0631265.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            32583..33380
                     /locus_tag="NOA01_000025"
     CDS             32583..33380
                     /locus_tag="NOA01_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HCI0631266.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            33364..33933
                     /gene="scpB"
                     /locus_tag="NOA01_000026"
     CDS             33364..33933
                     /gene="scpB"
                     /locus_tag="NOA01_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HCI0631267.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            33939..34655
                     /locus_tag="NOA01_000027"
     CDS             33939..34655
                     /locus_tag="NOA01_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCI0631268.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     regulatory      34737..34862
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            34985..35596
                     /locus_tag="NOA01_000028"
     CDS             34985..35596
                     /locus_tag="NOA01_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCI0631269.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     gene            35974..37497
                     /locus_tag="NOA01_000029"
     CDS             35974..37497
                     /locus_tag="NOA01_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HCI0631270.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            37734..39074
                     /locus_tag="NOA01_000030"
     CDS             37734..39074
                     /locus_tag="NOA01_000030"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HCI0631271.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            39227..39964
                     /locus_tag="NOA01_000031"
     CDS             39227..39964
                     /locus_tag="NOA01_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HCI0631272.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(40129..40350)
                     /locus_tag="NOA01_000032"
     CDS             complement(40129..40350)
                     /locus_tag="NOA01_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HCI0631273.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            40381..41412
                     /locus_tag="NOA01_000033"
     CDS             40381..41412
                     /locus_tag="NOA01_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCI0631274.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            41399..42802
                     /locus_tag="NOA01_000034"
     CDS             41399..42802
                     /locus_tag="NOA01_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCI0631275.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            42868..43503
                     /locus_tag="NOA01_000035"
     CDS             42868..43503
                     /locus_tag="NOA01_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCI0631276.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            43552..44232
                     /locus_tag="NOA01_000036"
     CDS             43552..44232
                     /locus_tag="NOA01_000036"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCI0631277.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            44367..45596
                     /gene="rpsA"
                     /locus_tag="NOA01_000037"
     CDS             44367..45596
                     /gene="rpsA"
                     /locus_tag="NOA01_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCI0631278.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            45778..47088
                     /gene="der"
                     /locus_tag="NOA01_000038"
     CDS             45778..47088
                     /gene="der"
                     /locus_tag="NOA01_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCI0631279.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            47331..47606
                     /locus_tag="NOA01_000039"
     CDS             47331..47606
                     /locus_tag="NOA01_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HCI0631280.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            47841..48887
                     /locus_tag="NOA01_000040"
     CDS             47841..48887
                     /locus_tag="NOA01_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631281.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            48889..50148
                     /locus_tag="NOA01_000041"
     CDS             48889..50148
                     /locus_tag="NOA01_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCI0631282.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            50154..50735
                     /locus_tag="NOA01_000042"
     CDS             50154..50735
                     /locus_tag="NOA01_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HCI0631283.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(50817..51692)
                     /locus_tag="NOA01_000043"
     CDS             complement(50817..51692)
                     /locus_tag="NOA01_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCI0631284.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            51840..52172
                     /locus_tag="NOA01_000044"
     CDS             51840..52172
                     /locus_tag="NOA01_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCI0631285.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            52284..53492
                     /locus_tag="NOA01_000045"
     CDS             52284..53492
                     /locus_tag="NOA01_000045"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HCI0631286.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            53646..54101
                     /locus_tag="NOA01_000046"
     CDS             53646..54101
                     /locus_tag="NOA01_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631287.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            54463..54738
                     /locus_tag="NOA01_000047"
     CDS             54463..54738
                     /locus_tag="NOA01_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631288.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            54751..56829
                     /locus_tag="NOA01_000048"
     CDS             54751..56829
                     /locus_tag="NOA01_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCI0631289.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
BASE COUNT        18931 a         9801 c        11986 g        16113 t
ORIGIN      
        1 aataaacgac cactattttc tgatttattg caaatgtttt ttcaaataaa tttgattacc
       61 aagaaaaggt agtggtatat ttactgaggg atactttgaa aaacatatga tagaaacagt
      121 aacagtatta tgaaaaatct ttttataaaa atggaggaat cgatttgatg gaagatcagt
      181 atgtacaagc aatgacgatc gcaggatcag atagcgatgg aagcgcaggg atgcaagcag
      241 atatgcatac attctttaca agaaatgttt acggtgtttc tgtcatgacc gcatgtgttg
      301 caggtaattc ttacggaatc ggagcaagtg tgacattgcc aacagatttt atcgacaaag
      361 aatttgaact gattgcgaag gactttcaaa tacgtgcagc taaaacaggt atgctagcag
      421 attcaaagtt gattgagaca gttgtaaaaa attaccaaaa atatgatttt ggaccattag
      481 tcgtggatcc cgtaatcgta acgaaacatg gaaatctttt actagaagaa tctgctttgc
      541 aaagcttgaa agaaaagctt gtccctttgg ccgaagtact gacacctaat ttttatgaag
      601 cagaaaagct gactggacgg acattagcag ctgaagaaga ttttgtgcaa gcagcaaaag
      661 aactgcaaga tatgggtgca aaaaacgtta tgatcaaagg acatcatgct aaaaatgcgc
      721 agcaagaaac agtcaatgac tacgtattgt tcgaatcagg aaaatcattt tggttaaatg
      781 caccatttta tcctaccgac cggatcaacg gaactggcga ttcgctatct gcatgtattg
      841 cagctgagtt agcgaaagga aaaacagttg aacaagcgat ccgattggca aaagactatg
      901 taaaccgtgc aattcgtgaa gaaataaatg tagggcacaa atatggcccc atcaatcatt
      961 gggcagctgg tgaattagag taaaagaaag aagttgtgaa aaagttgccc tgcatcaagt
     1021 aataagaaca gccaagtaaa aacagcttct tatgttttta acttggttgg acttattacc
     1081 gcagatgcaa gcttttgaac accgtttaag aaagaggtcg tgagcctgcc gtccagctcc
     1141 gagtcacaag gaacaagttc aaaaaacagc ttctcatgtt tgttgaatat tgtgacttgt
     1201 gaccgaggag ttggcaggag aacaccgttt gttcggaaga agggactgtg acaagatttt
     1261 tgtcacagtc cctttaatct tagtatattt ttctctactt gaataaaaaa attataggat
     1321 tggagaaaat aatcgagaaa tttgttgctt gaataatagc cagttagaca tttccttgac
     1381 tgtttcagtt gtcatggatg aacaatgtgt ctcatcttct ttgaactgag tagcaagttc
     1441 ttctaaaaac tcaggatcgt aaatgacagc acttgtttca aaattcaatc ggtagctacg
     1501 aatatcttga tttgctgaac ctaccatgca gatttcatca tccattatca atgtttttgc
     1561 atggaggaag ccgttctcat agacaaggat ttgtacattt tccttgatca gctgccgagc
     1621 ataatattgt gttgctcgat aaataaaagg atgatccggt ttgttgggaa tcatgatttt
     1681 cacatctaca ccagaggcag ttgcgatttt taaagcagca atgacacttt catcaggaac
     1741 aaggtaaggt gtctgtatcc aaacacgttt tttagcagaa gtaatcaatt tgataaatga
     1801 aagcttgatt tgttcccttt cgttattcgg tccgcttgaa acaagctgga tggatgtatc
     1861 agcaagctta gattcatctc gatctgcttt tttaaagaaa taattcaaat gatagccgac
     1921 ctttttttct tcaggtaccg agacgttcca gtctgttaag aagcgaagct ggagcaaaga
     1981 agaagcggca ccgaaaatcc gtatatgcgt atcgcgccaa tagccgaatt tttttgttgt
     2041 atttacatat tgattggcaa tattgaaacc accggtataa ctaatctttc catcgatcac
     2101 aacgattttt cggtgatcat ggtaattcaa tcggaaacga agtaatgccc tttgtgaggt
     2161 aacaaatgta tggacatgtc caccattttt gattagacga ttgaaatctt ttgcttttgt
     2221 gccatgagag ccaaatgcat catataatat tcgaacttcc acgccttctg cggctttttc
     2281 ttctaaaaca tgtaagactt gctggccgat attatctgtc acaaatgcgt aatattcgat
     2341 atggatcgaa tgtttggctt tttttagatc ttgaagcagt gaatccaatt tctcttgtcc
     2401 gtctgtgaga agagtgacag aattcattct tgtcagcggc atacgattta atgaagagaa
     2461 gaagtcaaca aattgctgtt tgtctttatc gcccatgtca ggatcgtaat gttcaatggt
     2521 atctcctcta aaagactgaa agttttctaa ttctttcaag tcactttgtc ggagatagaa
     2581 ttttttctta tccgttaatc cacgtccgaa aaataaatat aatacaaagc ccaccccagg
     2641 aagggcaaac aatactaaga gccatgccca aatggctgct acatctcggg gtttgatcaa
     2701 aatagccacc aaagcaataa gtgcatttaa tagataaagg gcggttataa tactagatac
     2761 cacaaaaatg ttcctcctta catacaataa cattagtata gcatgataga agaaagaagc
     2821 ctaccaaggt gaatatactt ttcactcctt tcatgaaaat gtaggtaaat cgggctaaag
     2881 tagttgcttt ttgtaggtta ataaggtagg atattagtac agttgaatat ctccttaggg
     2941 gagtaaccgg caacgtttgt tgcgataata tcaacagtaa gttaaccaaa tggttttctg
     3001 gtattgtatg aaatggtgag acctaagaca aatgcgtatt tgtcttaggt ctcttttttt
     3061 tcagacagga ctagggccaa gatcaaaaaa tgggggatga aatttggaaa acaaggaaca
     3121 agcacaacaa gcggtctata aagaaacgat cgactcgttg atgcagcaaa cgcagagctc
     3181 aaccgatgga ttgagcacca cgcaagcgaa ggaaaaatta gaaagagatg gcttgaacca
     3241 gctaaaagaa aagccgaaga agccaacttg gaaattgttc ttagaaactt tcaaagatgc
     3301 aatggttatc gtattgctga ttgtcgcact gattcagatg gtcatgggtg actacatcga
     3361 attcttagtt atttttgcag tattgatgtt gaattctata atcagtgttg tccaaacaaa
     3421 aaaagcagaa ggttctttag aagcgttgaa aaacctttca gcgcccgatg ctcgagtgat
     3481 tcgtgatggt aaggaaaaaa cgattccggc aaacgaactt gtggtaggag atatcgtgtt
     3541 gttagacgca ggcgattatg tacctgctga tggacgttta ctagatgcgg gatcattaaa
     3601 agtagatgaa ggtatgctga caggagagtc gacttctgca gaaaaagaag taacggatat
     3661 cacaagtacg gtaccaatcg gagatcgtac aaatatggta ttcagtggaa cgatcgtaac
     3721 ttacggacgc ggtaaattct tagtgacttc aactgggaat aatacggaga tcggacaagt
     3781 tgctggcctt ttggaatcaa caactgagca agtcactccg ttacaacgag gtctggataa
     3841 attcagtaaa aaactaagtt tggctatttt ggcattgtct ttattgattt taggtatcca
     3901 actgtttcgt atttatcttg gagaaggtac aggagatatg actgcagata tcgtcagcgc
     3961 agtgatgttt gccgtagccg tagccgtagc tgctattcca gaagcattac agtctatcgt
     4021 aacaatcgtg ttatctttgg gaacgaataa aatggcaaaa cgtcatgcca ttattcggaa
     4081 gcttccagca gttgaaacac ttggttcgac aagtatcatc tgtacagata agactggtac
     4141 attgacacaa aacaaaatga cagttgttga ctacttttta ggtaatggcg atactgggga
     4201 tttcacaaat gacccttcaa aatggacagc tgacgaacgt cgcttgatcg aaatcagtgt
     4261 tttagcgaat gatgcatcga tcagtgaaga cggtactaaa ttgggcgacc ctacggaagt
     4321 agcgtttatc gactttagtg aaaaattaaa ccagccttat caggaaatcc gaaacaacta
     4381 cccaagacaa gcagaacttc cttttgactc agatcgaaaa ttaatgtcta ctggacacac
     4441 aattgataac aaaaattact tgtttgttaa aggcggacca gatatcgtct tccaacgcag
     4501 taaaaaagta ttgctaaatg gtgaagtcgt tccgatgacg gatgaattga tgaaaaaatt
     4561 ccaagagcaa aatgaagctt tttcaacacg cgcattacgt gtactagctt ttgcttaccg
     4621 tccacttgat aattttgatt tatcatttga agatgaagac gatttgatcc ttgttggttt
     4681 gcttgctatg atcgatcctc cacgtgctga agtgactcag gcggtaacag aagcaagaag
     4741 tgcaggaatc aaaacaatca tgattactgg tgaccacaaa acaacagctg ttgctatcgc
     4801 aaaacaaatc gggatttttg aagaaggaaa cctggctttg accgggacgg aactagatga
     4861 attgacagaa gatcaactaa acgaagtttt agaaaaagtc actgtttatg cacgtgtttc
     4921 acctgagaat aaaattcgta tcgttcgtgc ttggcaaaat aaaggtcata tctcagctat
     4981 gacaggtgat ggtgtaaatg acgcaccagc attgaaacaa gcagacatcg gaattgctat
     5041 gggaacgggt acagatgtgg caaaagatgc agcagcgatg gtattgacgg atgataactt
     5101 tgcttctatt gtcagcgcag tagaagtagg acgtaatgtg tttgataata tcaagaaagc
     5161 tatttcttat ctatttgcag gaaatttagg tgcgatcatt gcgatcatct ttgccttagt
     5221 tgctgactgg tccaatccat ttacagcatt acaactgttg ttcatcaatt tagtcaatga
     5281 ttcactgcca gcaattgcat taggtacaga aaaagctgaa cctgctacga tgaaccgccc
     5341 gccaagagat ccaaattcag gaatctttac ggacaaaaca ttgatttctg taacgtatcg
     5401 tggtgtgctg attgggattg ctgttatctt tgctcaatgg cttggcatgc agcattcacc
     5461 tgaattcggt gtagccatgt cattttcaac attgatttgg tgtcggacat tgcaaacatt
     5521 cccagcacgt tcaaacacac aaacagctat ccaagcaggg ttcttctcta acagaagtgt
     5581 cttactagct gtagttgtat gtagcgcgct ttactgctta acaatgatcc caggactgaa
     5641 agaagtcttc tctatcccat cagcatttgg attgacagaa gtctttacat gtatgggaat
     5701 cgcacttgtg acaattgttt tgatggaaat cactaaattg atcattgtac gagtacaagg
     5761 aaataaataa aacgaatcaa cgaggttgtg agcctgccgt ttaattctga gtcacaaggt
     5821 gcaatttcaa caaacagctt ctcatgtttg ttgaatattg tgacttgtga ccgaagagtt
     5881 ggcaggagaa caccgtttaa ggaagaagtt gtaaaatcag cattttgagc taatactgag
     5941 ggtcatctga ttggacactg tttattcgga aagagggact ttgacacgac tttggtcaca
     6001 gtcccttttt ttatttatga tagacggaaa gccaatcatt ttattattca tcaaaagaga
     6061 tagaatagat aaaagaggag aatgatttat tccgaaaagt gaaaacatat aacaaaaaat
     6121 tagaggaaat caaatgaatg aaaaagaatg ggatgaattt gctgaagaat attatgaaat
     6181 ccagcaagaa tcgcaagtaa ctattgctga agatgtaaaa aattatctga aaacggagaa
     6241 agtcctgcct gctgaaacag cagcagacgt tgctggagga tctggcagat ttctttcctt
     6301 agcaaaagaa attaatcgct atgatttgat cgatttttct tcaggaatgc tagaaaatgc
     6361 aaaaaaagaa gcagaaaaaa atgagttgcc aaatgtttcc tttatcaaac agtcgttaga
     6421 agaatttatc caatcaaatg agacatatga aatggttttt acagcagcaa atccagcatt
     6481 ggtacatctt attcaattag aaaaattgat gaaaaagtca agcaaatggt gcgtgataat
     6541 cagagtagtg gaatcaatcg atgatgtttt ttatcctatc gaaaagcaac taggaatgat
     6601 agaagaagat cctaatacat cgccagttgt aatggatcaa tttgaaacat acttaattga
     6661 tgaaggattc aaagtgaaga aaaaagattt tacttatttt acacaggaat caattactga
     6721 agaattgatg aagctatact atgacgcgta cgcaaaagat cctgttgtga ccaggtacat
     6781 tcgcaaacta ttcagtgaaa aacgagaaat ccttagtacg actaagctga cctatagact
     6841 gctcctttgg tccgcagata aaaaaagaac ctttcacgac tcgtgaaggg ttctttttat
     6901 gattttatta tattttgtag taacgaatat cattttcgat ctttgtaatc gaaaaaggta
     6961 catctgaagt atattctgca cattctccgt gttcaggaag cgtctcactg aaaatcgttt
     7021 catattcgtc aacagtgatc cgtgttcgag aatctagcat ctcttgatga gattgtgcga
     7081 agagatattc ttgatagttt tcttccagat aacctgtaaa aaattcactt actgcaccag
     7141 aaccgtagct gaatagaccg atgcgatctc ctggctgtag tgatttcgaa ttttccagta
     7201 gagaagttag ccccaggtat aatgaaccag tgtaaagatt accgattcgt cggctgtaac
     7261 ggatgctttc ttcatagcga gccataagac gttcctgatt ttcttcgtct gtttggtcta
     7321 atacgctttg caatgccttt tttcccatct tagtatacgg aatatggaaa gcaatcgcct
     7381 gataatcttc gagtcctcga cccgacaatt ctttatgtcg attccaaact ttttggaatg
     7441 attcgatata cgtagaatta gataaaggac catcaacaac aggaaattcg ctataatctg
     7501 gacgccagaa atcatagata tcttctgtca gaaatacgct gtcgtcttca atcgataaaa
     7561 tacgcgggtt ttgagtgatc atcatcgcaa cagcaccgac accttgcgtc acttcaccac
     7621 cgcttgccaa gccgtaacga gcaatatcac ttgctatgac tagtactttt cgttctggat
     7681 gatttttgac atattctttc gccatgtgca aagctgcggt gccaccgtag caagcctctt
     7741 ttacttcaaa agaacgagca aaagattgga ttttcagtaa atggtggata attaccgcac
     7801 tcgcttttga atgatcaatc ccggattctg ttccgacgat gaccatgtcg attagctcac
     7861 ggtctttttc tgttacgatt ttgctggcag catttgctcc tagtgtaatg atgtcttcat
     7921 ttgcacgatt gactgccatt tggtcttgac caatccctat atggtatttt gcaggatcat
     7981 ccccacggct ttctgccaac tctgtcatat ctaaatataa attaggaata aaaaaggaaa
     8041 gacgatcaat ccctattttc atttcaacac ctctttactt atgataaaaa acttacttta
     8101 ctaaggtatc ataaaaagat taaaagaaat ataaaaaaga gaatatttaa ggattgttaa
     8161 aatttaatct tagaaaaaaa taactggaac aagagctatt tgtttttaca gcaaagaatg
     8221 gtatattttt gtaggaggtg gcttacttga aagaagtcgt tatgatagat gctgcaagaa
     8281 cgccgatagg aaagtatcgc ggaagtttaa gtccatttac tgcagttgaa ttaggcacac
     8341 ttgtgactaa aggattatta gataaaacaa aactgaaaaa agataaaatc gatcaagtaa
     8401 tatttggaaa tgtcctgcaa gctggtaatg gtcaaaatgt tgccagacag attgctttga
     8461 acagcggtct ccctgtagat gtcccagcaa tgacgatcaa tgaagtttgc ggatctggta
     8521 tgaaagcagt gatcttagca cgccagctga tccaattagg tgaagcagaa cttgtgatag
     8581 ccggcggaac agagagtatg tctcaagcac ctatgctgaa acagtatcag tcagaaacaa
     8641 atgaatatgg tgaaccaatt tccagtatgg tcaacgacgg attgactgac gcattttcaa
     8701 atgcacatat gggattaacc gcagagaatg ttgcaacaca attttctgtg agcagagaag
     8761 agcaggatcg ctatgccttg tcgtcccagt tgaaagcagc acatgctgtc gaagccggtg
     8821 tattttctga ggagatcatc ccagtcaaga tttctgatga agacgtgtta tctgaggatg
     8881 aagcagttcg tggaaatagt acattggaaa aactgggcac gttacgtaca gtattctcag
     8941 aagaaggaac tgtaacagca ggaaatgctt ccccgttgaa tgacggtgcc tctgtggtga
     9001 tccttgcatc caaagaatac gcagaaaata ataatctgcc ttatttagca accatcaaag
     9061 aagtagcgga ggtcggtatt gacccaagta tcatgggaat cgctccaatc aaagccattc
     9121 aaaaattgac agatagatca ggaatgaatt tatcaacaat cgatcttttt gagatcaatg
     9181 aagcatttgc agcttcttct atcgtagtta gccaagaact gcagttagat gaagaaaagg
     9241 tcaatatata tggaggagca atcgcattag gccatccaat tggtgcaagc ggtgcacgta
     9301 tcttgactac attagcttac ggattgcttc gtgaacagaa gagatacggt attgcttcat
     9361 tatgtattgg tggaggttta ggcttggcag tcttgttaga agcgaatatg gagcaaacac
     9421 ataaggatgt gcaaaaaaaa aagttttatc agcttacgcc atctgaacgg cgcagtcaat
     9481 taatagaaaa aaatgtattg acacaagaaa ctgcgttgat ttttcaggaa caaacacttt
     9541 ctgaagagct ctccgatcac atgatcgaaa accaagtgag tgaagtggaa atcccaatgg
     9601 ggatcgcgca gaattttcaa atcaatggca agaaaaaatg gattccgatg gcaacagagg
     9661 aaccatctgt cattgcagct gcaagtaatg gtgcaaaaat ctgtggaaat atctgcgcag
     9721 aaacacctca gcggctgatg cgagggcaaa tcgttctatc aggaaaatcg gagtatcaag
     9781 cagtgatcaa tgcagttaat catcgaaaag aagagctgat tctttgtgca aatgaaagtt
     9841 atccttcaat cgtgaaacgc gggggcggtg ttcaagatat ctctactcgg gaattcatgg
     9901 ggagtttcca tgcatactta tctattgatt ttttagtgga tgtaaaagat gcaatgggcg
     9961 ctaacatgat caactcgatt cttgagagtg tggcaaacaa gttacgggag tggttccctg
    10021 aagaagagat tctattcagt attttaagta attttgctac agaatcattg gcttctgcct
    10081 gttgtgaaat cccattcgaa cgcttaggga gaaacaaaga aatcggtgaa caaattgcca
    10141 aaaaaatcca acaggcaggg gaatatgcga agcttgaccc ttaccgtgca gcaacgcaca
    10201 acaaagggat catgaatggt atagaagctg tagtggcagc tacaggaaat gatacacgtg
    10261 cggttagcgc ttcgatccac gcctacgcgg caagaaatgg attataccaa ggattaacag
    10321 actggcagat caaaggggat aaactagttg gaaaactaac ggtgcctttg gctgttgcca
    10381 cagtaggcgg agcttccaat atcctgccaa aagcaaaagc atctttagct atgttagata
    10441 ttgattcagc caaggaacta gcacaagtaa ttgctgcagt agggttagct caaaacctgg
    10501 ctgctttgcg tgcattagtt acagaaggaa tccaaaaagg ccacatggga cttcaagctc
    10561 gttcattagc gatctcaatt ggggctattg gtgaggaaat cgagcaagtt gcgaaaaaat
    10621 tacgtgaagc agaaaagatg aaccagcaga cagctataca aatattggaa aaaatccggg
    10681 aaaaataaaa aagcaatatc tttagaagaa taaagcaaga tttacagtta ttttatttac
    10741 tgcagatctt gcttttttct ctatcagaaa atatgtttat tattagaatt ttttatagtt
    10801 aaggttaggc atcttaagaa atcaggcaac tccaagatat cttcaggtag aagagaagtc
    10861 gcctaacatt gtaggatcat aggtgatttg ttggacaggt caagctgata tttgagcctg
    10921 atgttagaag gaatttcttt ttacttgctg tagaaattat tatatgacta aaaaggctgg
    10981 gattttaaat cccagccttt tatgacaaat agaattttta tcgatcagta atcgctgtcg
    11041 ctgcaaattg ttcgatcaaa ggaatcaaag tcaatttgac tttgtatttc cgttcgagtg
    11101 ctttttcgat aacgaactca ttttcagtag tcatagaaat agttgctaat ttacgaggtt
    11161 catcagcatt tttttcatta ataactactt catgttcatt ttgttctgtg taaaatttag
    11221 gatactcagc aacggctttt gaaaaatcag caaaacttaa agcggcattc gtatcagttg
    11281 agcgtgtatt ttcagttgtg tccgcttttg gtttttccgt tttttcttct ggcacactga
    11341 ctgtttcttc tgtaactggt gtcgcaacag cttgcgcagt tggttcatcg tttttcaatc
    11401 gttgatcatt ccagtaagac acgatttcaa taagaagatt gtcacggaat cgttcgaaaa
    11461 tcccgttttc tcctgaacga attctcttta atacagtatt gaagttgtca tcgattggca
    11521 cgttgatccg tgtatcttca tttgttttcc cagctaatcc gatcgttaag atttgacggt
    11581 ttttgggatc aagcatgact tgtaacggca aaccataacg tcgcaagctc tcttcttcta
    11641 gagaacggaa taattgtcct cttacaccat ccaatgaagg acggaattga tttaataatt
    11701 tttgtttctg ttttttcttc atatgttctc tccttcgctc ataccctttc gattataaga
    11761 aaaaaacagc caaatgaaaa ggagtaatgc aaaaaaactt gcgtatacgc aaattacaac
    11821 agaaaaattt gcgtattttc ccgttttttg aaagaaaaat aaaaaatgga acggattatg
    11881 tatatataca taatccgttc cattctttct attttttcgg tatcaaggca aaaacaggta
    11941 gttcaggtga ataaatgact tcatgttcgt gccagggttt gaccattagc gaatgatcga
    12001 accgtttttt cagttcttcg atgatttgat catgtgtcca gtttcttgca ggaaaatgtt
    12061 ggattttctc tgcatcgatc aaaaagacaa aatcggaatg aaaagtctgg gtaacttctt
    12121 ccgagagaga attcaagcgt tcgattggat ccataaaatc acctttcgac ttaaaattgg
    12181 gtagtatctg ggtcttttgc tttgccgact acaccatcta actgatcgat ttgtttcatt
    12241 tcttcttctg ttaattcaaa atcaaataat tcagtatttt ctttgattct gcttggtgtc
    12301 actgacttag gaagaggaag gaattcgtgt tgcagcgacc aacgcaaagc aacttgtgca
    12361 atcgtcttgt catgtgcttc agcgatttgt ttcatttcag gcacatcgaa gattttacca
    12421 gtgcctaatg gactatatgc ttcgaggatc atatcatgtt tttttgcata ctcaactaca
    12481 gttgtctgta attcgcctgg tgcaaggaaa acttgattga ccatcggcat aatttttgct
    12541 gtttttgcta gtgtatctag atggtggggt aagaaattgc tcacaccgat tgctttgatt
    12601 ttacctgctt catacagttc ttcaaatgct cgccatgtat ctgcatttgc ttgttcccaa
    12661 ttatcacgaa aagcaacagg gtttggccag tggatcaaaa acagatctaa ataatctgtt
    12721 tggagcttct tcaatgattc ttcaaaagaa gacatcacaa gttcatagct gtggtttgca
    12781 ttccataatt ttgttgtcaa gaatatctct tctctaggaa ttccactttc cttaatcgct
    12841 tggccgacgc tttcttcatt cttgtatcct tgggcagtat cgatatggcg ataacctgca
    12901 gccagcgctt ctttaacaga ggaaacagcg acatccccat caggtgtttg ccatgtgccg
    12961 aatcctacta ccgggatttc gtaaccattt gatagacgat aagtgcttgt taatgaattc
    13021 atactttcag ctcctttttt attcgttcaa ctataagtgt agcgcatttt tcgaaaaaaa
    13081 gaatccattt tgtttctact cttgaaattt ttgtttaaaa gaaaatgatt ttgattctgg
    13141 tatttttgat tttttttccg taaactaaga cagaagggaa tgagaaaatg gatatctatt
    13201 taaaaaaagc aattctgcat attattgaca gagaaactgg ttctccagtc ttttcacaaa
    13261 aagagctgga tctgacaaaa gaatatattc gtgattttct tcaaaagaag atccaaaaaa
    13321 tctcttctgc tcaaacaaaa actggacaac tagcagaaga aagcatgatc gttgaatcac
    13381 ttaaaatcgc tcaagaaaat tttgtggaag caagtgaagc aatcgttcag cggtggttcg
    13441 atatctacca agaaagtgaa gaagcaccta gtgcagatgt atttgttgtc ttgtatgaat
    13501 tagatacagt gatgtactta tctttattga aagtcaatta tcgtgaggct tacactcact
    13561 ttgttgaagc agacgaagca gggatcgaca accgcttgat tttgaatcga gcaattctag
    13621 gggcgaaaac acaaaaagca gatgaagcat tcgctgtgaa tttgactgat tggacctacg
    13681 aattaattga aaaaaaatat gagttttctg gcaaaaaaga gctttatttt tctggaagag
    13741 tgatcgaaag tgttcctgca ccttctttag aagaaaatgt aaaagtgatc caaaaagtag
    13801 ccaagcatct tggaaaaaaa tttgagacag aagaatttga tatcatggct gatttgaaag
    13861 aagctgtcta tgatacgata gaagaaaaag gtcggttgga tcatgagatg attgcggaaa
    13921 aagtcttcaa ggaaaacatc acggcaaaaa tggcttttaa agaagaagta caagagcaag
    13981 gatttgtccc agaagcacct cctgtaaaag aagtacaaga aatttctgag aaaaaatttg
    14041 gaaaacaaaa attcaagcta tcaaacggga tcgaactgat cgtaccagta gatgtatatc
    14101 gcaacccaga tttgattgaa tttgtgaata atccggatgg tacgatatcc gtgatgatca
    14161 aaaatgtgga tgaagtattg aatcgtttat aaaaatgact agaatattag gtgttcaagt
    14221 accagtgatc agttaaatga attgtgtcaa gagaaggatc ggaaaagacg aatataacaa
    14281 aaaaagagcg tgagacaaaa gtaaagaaca cttttgtctc acgctctaaa cacgtataaa
    14341 cggcggaagc agaagcaact ccttcttatt tcttggagtt aaacgcttct gtcccagcct
    14401 ctttttatct aataaaattt taagaccatt tactaattat cgattttttc tgccgcattt
    14461 ttaggcactt cagaagcctt tatttcttcc cagcttaata cacctttacg aggattgact
    14521 ttgagtttaa gatacgcatt catctttaat ggatgttctc tttcttcgcg aagcgtcacc
    14581 tgtttttttt caccatcttt attataagcc gcttgcttgt aggtataagt cgtgaatttc
    14641 tctccgttat ctgcttgggt agctgacatt tcgccattcg tcgtgatttt tgtataataa
    14701 gcttcgccgc cgtaaaaata atgataagct gcaaaacttc cgccgagaaa tatgcctaaa
    14761 acgacaagta cgccaattaa ttttttcatg tacttcacct ctttccttat tattaacgaa
    14821 atagaagatt tcgtctattg aaagttgttg gcaacagctg aattttaaac ttttcctcat
    14881 tttgttctta ttgcttcaaa agaaagttaa ggactatctt gaagtttttc tcaaaaaaca
    14941 cattttttta tttataaaag gtactttttt cgcgtataag acagacgagt gctcttagtt
    15001 gactttgacc aatattgacg tataataagc ttagttcgaa tcttacaaaa agtaagtcgg
    15061 acgtattctt tttgatttag tcaaaaaaag actataataa aaaggtgaga gctgaaattt
    15121 ttcagtgatc tatattattt aggaggtttg taaattatgg caaaagaaca ttatgacaga
    15181 agtaaaccac acgttaacat tggtacgatt ggacacgttg accatgggaa aactacttta
    15241 actgctgcga tcactacagt attaggcaaa aaaggcctag caaatcctca agactatgct
    15301 agtattgatg cagctccaga agaacgtgaa cgtgggatta cgatcaatac cgcacacgta
    15361 gaatatgaaa cagaaaaacg ccactacgcg catattgatg caccaggaca cgcggactat
    15421 gtgaaaaaca tgattacagg ggcagcacaa atggatggtg caatcttagt tgtttctgca
    15481 actgacggtc cgatgcctca aacacgtgaa cacattttat tgtcacgcca agttggtgta
    15541 aaatacctga ttgttttctt gaacaaagtt gatttagtcg atgatgaaga attgatcgat
    15601 ttggtagaaa tggaagttcg cgagttattg agcgaatatg gtttcccagg cgatgacact
    15661 cctgtgatca aaggttccgc attaaaagca ttgcaaggcg atccagatgc tgaagctgct
    15721 attatggaat tgatggatac agtagatgaa tatatcccaa caccagaacg tgatacagat
    15781 aaaccattac tattgccagt ggaagacgtc ttctcaatta caggtcgagg aactgttgcc
    15841 tcaggtcgta ttgatcgtgg tgctgttcgt gtcggtgatg aggtagagat cgtagggatc
    15901 aaacctgaaa cacaaaaagc agttgtaaca ggtgtagaaa tgttccgtaa aacgttagat
    15961 tacggggaag ctggggataa cgtaggcgtg ttgttacggg ggatccaacg tgacgatatc
    16021 gaacgtggac aagtacttgc taaaccaggt tccattactc cacatacaaa attcaaagca
    16081 gaagtgtacg tgttgacaaa agaagaaggt ggacgtcata ctccattctt caacaactat
    16141 cgtccacagt tctacttccg cacaactgat gttacaggaa caatcacatt gccagaagat
    16201 acagaaatgg tcatgcctgg tgacaacgtg acgattgatg ttgacttgat ccatccaatc
    16261 gctgttgaaa acggaacgac attctcaatt cgtgaaggcg gacggacagt aggttcaggt
    16321 atcgttacag agatcgaagc ataagaaacg cgactcgaat attttggtaa tgaaaaatgt
    16381 ttaaaataaa agagcggtcc aaacaggatt tctgtttgga ccgctctttg ttcttcaatt
    16441 gtataagaag agattgtaga agcatgaact ttcgttttac ttcagtctct tttattcatc
    16501 atgagagtgt tcatctatcg gtatgcagtt tttaatactt tcttcgtatc cattttttca
    16561 aaccacttat tctaccttgt gtctgctcaa ctggcgcatt atcaataatc agcaaagcaa
    16621 tcataacata tgcaatccaa ctatctctag aatatagagt ataacgtgag acccagtttg
    16681 tagcgtactt gcttttatat tctcttaatc catggaaaga ataaaaatga gaaccaaact
    16741 cgtaaaccaa gtatgcaatc ctttcctgta aaaaactctt tctggaagtt ccgacatttg
    16801 aaagaggggc catccctaaa ttgaaccatt ggatatgttc ttctttcata taggcaaaca
    16861 aatgcaagaa caagaaatcc atactgccag aaggtgcttt tttagaatca tatcgcatca
    16921 gatcaatcgt tcctacttta ttgttcgtgt aagtaggcat gatcgttgca aaagcgacaa
    16981 tttcttcttg ggtatttttg acaactgcaa taggcgctct ttgtagataa tcttcagaga
    17041 aaaagcctaa agaaaaccct ttttcctttc gtgaacccaa ccaattatca gagatatttt
    17101 tgaaagtact catttgctca gtagaaaatg gtggttgtaa gacatcaaat gtaaatcctt
    17161 ctcgttcgat ccgattcaag acagcacgag tgcccttcat ttttttccct gaagttgtaa
    17221 aactattcag gtctacataa gcttcttccc ccatcttaat gaaatcgtag ccgaattcat
    17281 gcaaaatcat aacaatctct tcgcttgttt cgtagaaaac tggtaaatag catagtcgat
    17341 ctgtttcttc gataaaagct tcgattgctt ctggaaaatc ttctttctta ccagaaggat
    17401 cacccattac aatacattta ttattgaagc aagcgaattg gagaagaaca gttggttcac
    17461 cgtctttttc ataggcaaac atccgcttat cttttaagaa gatcaattga ctatcggagt
    17521 ttcctccata agttgtaagg atcttcaggg ccttttcttc attaaaggct tcgccaatct
    17581 gtttcttttc tccttgaagg aaatgaacaa atagaacgat catgaaacta acagcgatga
    17641 tggccaacaa tcccgaaaac caaatctttt cagaaggaaa taagaaaaag gaaataaaat
    17701 ggtgacgccg atgagggaaa tctggcagat tgtatacccc aataacgatg taaagcaagg
    17761 acagtgcacc aataatgatt ccatcgatcg tacgccactc ccatgaatag atcaattgtt
    17821 cccgaaataa ttcgttttta gaagcaagaa tgatcagaag cagaatagac aagaaaacca
    17881 ccggtgtgaa actaaaatca cttaataaaa cataaagtaa agctagaaca atcaagaaga
    17941 ttgtaggcaa atatgctcgt ttcacacgtg ctgcgatccc tctgcccata atgattaaaa
    18001 gaaatcccaa tagtatacta ggaaattgga taataaaatg aaatttcaat gggttcaaac
    18061 tatgaagcca gcgaaactct gtaaaagctt gcgggatagt ggcagaaagt actagcatga
    18121 ttccagaaaa gtataataag acaaccacga ttttatgcgc aatttctgtt gctaattgtt
    18181 tagggatacc agaataacgc tgatcaaaag tggaaccgat atttttgaag aaaaagacga
    18241 ttccaatcaa aaacggaatg atgtagtaga acaggcgata gagcaagatc cacacaacga
    18301 ctatttctct tggcacacca atagcggaca acccgataat catcattaca tcgaaactcc
    18361 caatttctcc tggaatcata gacacgattc ctatcactga agcagcaatg aataatggaa
    18421 ttgtttgcca cagatcaatc cggatttcca ttaaataacc aacagaaagg aaactaaaca
    18481 ggacaccggt ccattccaac aatgaaacta gcaatagctg tccttgagtt ttaagtgagg
    18541 taccaccaag gaatcctttc tttttgaaag tagtaaataa aaagacgaca ggaaaatata
    18601 gaccgccgcc gatcaaccat atccaatatt gcttcaaaaa aggagcaact ggcgtgaaga
    18661 ctacaagtaa aaaagaaagc agactgtaaa tagaaagtcc agccatcaaa aacaagaaaa
    18721 ctttcgataa tgcttggatc acgtttctgc tctctttctt ttgtccatat agttcagagc
    18781 gaagccccac actaacaaac ccgccgaaac cggcaatatt gttgatcgta ttgatcagcc
    18841 agcttgtctc aaacaggtaa cgaggattct gcttttgttc aagcatccga ttcaaaataa
    18901 tatcataacc aaccataggt aacacagaga taagaccaat caatagcatt agtccgcttt
    18961 tccatacagg tatagtggca aacgtttctc ctaattgctg gatagataga gttttaccga
    19021 tggacatcaa ttcaccgaca ataatgacaa tgacagaaat caaaaagatt gttttgaaca
    19081 gtcctaggtg gtttttcagc cattgtatgg tattttttaa caaataaatc accttctatt
    19141 cctaatagtt atgtttcaga aagaaattga agaacttctc gattgaagcg ttcaggatct
    19201 tttctagcta attcatgtcc ttgttctttc acaagcacaa aggaagcttt tggtatagtt
    19261 ttagctatat acaaagaatg ttttagcttg attacatctt tttttcccac gatgatcaaa
    19321 gtgggggagt tgattttttt taaatcattt tctgtaagcc cgatgtcatg cagcaaaagt
    19381 ttgataacta aaagattttt gcgaagactt gggcgaaata aagagagtaa ccatacccat
    19441 gcataatgaa tattactgat gactctggca gaaaaacgaa cgcctttaac aagcgtgttt
    19501 ccagaattta aaatcaatcg atgaactttt ttagggaagc tgctggcaaa taccagtgct
    19561 agatttgctc catcactaaa tccaagaaaa tctgcctgat cgattttttc cagcaacatg
    19621 atcgtattca gatcctcggc cattaactga aaattcaaca tagatgcttc gtttgtcgaa
    19681 cgaccatgtc ctcggctatc tactagatac acggtatagt agcgttcgag tacagggacc
    19741 tgttctgaaa aaaagcggcc gctgccgtca ttgccatgca gtaaaaagag agggaagcct
    19801 tgaccgctct tttcatagta aatttcgctc ccatctggca tttttgcata tttcttttct
    19861 tttttcacat aatcacctac tgtgtaaagt ataacgatta tatcagtcat tgggcaattg
    19921 tttttactga aaaacaacga tcattttcgt aagatgtcaa aaaatacagg aaaattcatt
    19981 ttttcttaag gcgtacacag aaatcaagga agaaatagcc aagaagataa agaaatagta
    20041 agataaaaaa agatgtcaaa ataactaggt gatgaaatga agcgaaaacg attatctatt
    20101 atccgtattc ttttttacat gatcattctg acggttattg gtgcggtgtt gtttttagga
    20161 ggaagaactt atcgttcggt aaaacgagtg gaaagctatc aaatagaaat aacaacagct
    20221 gcaaaaaatt atcatatgga aaactacgag aatttgatca aggcgataat attgaccgaa
    20281 tcaaaaggaa aagggacaga tctcatgcag agctcggaaa gtctgcatgg agaagctgag
    20341 atcattgata atccagctga cagtattgat caaggtgtca aatatttggc ggaaatgata
    20401 gtagaagcaa agaaacaagg atgcgattta tggacagctg tccaagcata taattttggg
    20461 aaagattata ttgcctatgt aaaaaatcat ggcggagaaa acacagtaga attagcagaa
    20521 gcgtattcta aagatgtgct gagcccactt ttaggaaatg aggaagggaa acaataccga
    20581 tattggcgga tccagtcctt gctttacaat ggcggctatc tctatcataa tggaggtaac
    20641 atgttttatc ctgatattgt caaaatgaat gaaaaaataa tagagaccta tgaaaaagtc
    20701 tttaagtaaa atagcaggaa atgttcaatc atgaataaaa ttaaatgaaa gaaggagtgc
    20761 gcaaatagtg gaaaaaaggc aaaaaatgga aaatcaagaa gtggggtaca caaccaaaga
    20821 ctggatgtta cggtttgtga agggaatgtt tatcggttca ggatttatct tgccaggagt
    20881 cagcggtggt gcattagcag ctgtctttgg catgtatgag cggctgattt cttttttagc
    20941 ccaccttaca aaaaatttca aagaaaatgt tttgtttttt ctgcctgttg gtctaggcgg
    21001 agtgacaggc atctttctac tttctttttt agtcagtttt ttattaggtg cgtatgaaac
    21061 aacgatatta tggttttttg tcggatgtat cgctggaact gttcctgcgt tatggaaaga
    21121 agcagggaaa aaaggaagag atacttctga tgtgatcatc atggttgtta cttttgttct
    21181 ggcttatttg tttttattat acggcgctca attatttgac gggcaagtcg atcagaattt
    21241 tggaacatgg ctgattgccg gcggattgat tggtttgggt atgatcgtcc caggattgag
    21301 cccttctaat tttttggtgt atatgggatt gtataaagcg atggctgacg gatttaaaga
    21361 agtacgactt gaagtgattg tgccgatcgg aattggtggg attctttgtg ttcttggatt
    21421 gtcaaaaatc atggattaca ttttctcaaa ggcatatagc aagctattcc atttcatttt
    21481 aggtgtagtt tttgcttcaa cgattatgat cattcctacc gattacgcgg gattgggagt
    21541 tgctggggta ctagcttgtc ctgttctttt tatagccggt acagcactag gttggtggat
    21601 gagccgtttg gaggaacaat ataagtaaag atagattgat aataaaaaca gcacacgggt
    21661 gattcaaaca aaaaggttgg catctttttt gtaaaatcat tcgtgtgttt ttctataaat
    21721 tttataaaaa caagttgttt ttaatcttgg aacacgctag acttaaacac gtgattattt
    21781 ttgaaagttg gcggaaaatg gagaaaaaga aaatgaatgg acttacacag caagaggtag
    21841 ttcagaaaat ggcttctggc cagcaaaatg actaccagga agatgcatca aaaagtacaa
    21901 aacaaatatt cagcgataat atattgacgt tattcaattt tctgaacttt ggtattggga
    21961 tttgtctgtt atttgtaggt gcgtattcca acatggcgta tttagcaatt attgtcgtca
    22021 atatcgttat tggaatttat caagaaatac atgcaagaaa tcttgtacgg aaattatcga
    22081 ttgtttccca atcagaagta ctagtgatcc gggacggtaa agaacaatcg atttctacta
    22141 aagagctggt attaggagat ttggtaaaaa tcggagcagg agaacagata ccagccgatt
    22201 tacaggttat atctggacgg gcagaagcaa atgaagctct tttgacaggc gaatctgatt
    22261 tgattgtcaa agaaactgga gctgaattgc tttctggaag ctttttgaca agcggttctg
    22321 tcatggctga agttatccga gtaggtgcag ataattatgc tgtccgcttg actagcgagg
    22381 caaagactca caaagctatt cgttctgaac ttgtagattc gattcgcaaa gtatccaaat
    22441 tcactagttt cgtgattatt cctttgggga tcatactatt tttggaagca ctgttcatcc
    22501 gcgatgcttc tatcaaaatt tctgttattg catctgcagc agctttgtta ggcatgcttc
    22561 caaagggttt ggtattgcta ataagtattg cattaacgac aggggtaacc aaactggcaa
    22621 aaaaacgaat ccttgttcaa gacatgtatt caattgaaac gctggcccat gtcgatacat
    22681 tatgcttgga taaaacaggg accattactg aagggaaaat gagtatccag cgggtagaag
    22741 cactacatcc gctgtacgag aaagccatcc cagaaattat ggggacgtat ctggcagaaa
    22801 gcagtgataa caacatcact atgcgggcta tgagagatta ttttgagctt tctaatcggt
    22861 atgatgtgac aaacaaagtc gcattttctt ctgatcgcaa atggggcgcg atggaatttc
    22921 cagaactggg agtcgtgtac gtaggagcgc ctgaacgttt ggtggaagaa gatcggcttc
    22981 cggacagtct aaaaacagcc caagcgaatg gctatcgcgt acttatgttg gggatcgctc
    23041 caaatcagca attgaacgaa gaaaaaccag catttgttga gccggttgct ctgtttgaaa
    23101 tcgatgatcc aatccgaaaa aacgctcaag aaacactagc ttatcttcat gaggaaggtg
    23161 tggatctgaa agtgatttct ggagacaatc cttttactgt ctccaatatc gctagacgtg
    23221 caggattacc gaattatgat gcgtatgtcg atctttctca aattactgaa gaaatggaag
    23281 tgcgagaagc tgctcatcgc tatacggttt ttggacgagt gagtccgcag caaaagaaat
    23341 tgctggtaaa tgaattgaaa gaaagcggca gaacggttgc aatgacaggc gacggtgtga
    23401 atgatgtttt ggcgttaaga gaagcagatt gcagcattgc tatggcagaa ggtgatggag
    23461 caacacgtca gattgcgaat ctggtattgt tggactctga ttttactaca ttgccagaag
    23521 tcctgtttga aggacgccga gtggtcaaca atgtgacgaa agtctcaggc atctttttta
    23581 tcaaaacgat ttattcattt attttatcga ttatatgtgc cgttacagca attggatttc
    23641 cgtttatccc tatccaaatc acgctgattg atttagcgat tgaagggtat ccttccttct
    23701 ttttatcttt tgaacaagat aaaagaaaaa tcacttaccg ttatttgcct acagcactta
    23761 ttaatgcttt accaaatgcc ttgcttgtag tagtgaatat cattgcggtg tatctacttg
    23821 gaaaaacatt cgcatgggga aatgctgaaa ctactacttt gatgtattac ttgctgattg
    23881 ggatcagttg catggcagtt gtaaaagctt gttatccctt caacccattg cgcatcttcc
    23941 tagcagtaac gacgatcgta gggatatatg tagctgcaat gctgttccat ggaattttgg
    24001 aagtaaactt agggttggga gagagcgtag gatacttctt tgtattcatg ctcgtaaata
    24061 ttgttcttcg agtattgtac tggaaggctg atattcaaaa tcagttactg aaaagagtag
    24121 caaattgatg cttaatgaga tagaaatgtg ttatacttga aaaagcaatg aaatatgata
    24181 caatcaaaca tgtataattg tgtcctttaa aatcgcactg atttcagtta gtgcgatttt
    24241 ttaccggtta ttctcctgaa atatcttctg ttaaacccaa aagatatcaa tagagcaggg
    24301 ggtgaaaaaa tggctcaacg gattcctcaa gaggttatcg aaacagtacg cagtcaaacg
    24361 aatatcgttg aggtgattgg acaatatgtc cagttgaaaa agtctggtaa aaattacttg
    24421 gggctgtgtc cttttcatga agaacgtaca ccgtcttttt ctgttgcaga agacaagcag
    24481 atttttcatt gctttggttg tggtaaaggc ggaaacgtgt ttacttttct tcaagaacta
    24541 gaaggtctaa gttttccaga agcagtggta aaagtggcag acttcgaaca gataccgtta
    24601 gaagatcaat ggcgtcagcg tactatagct gtccaaaatc ctgaatctgc tacggggaaa
    24661 ctgattgctg ctcatgaaaa agcagcggaa atctatcatc acatgctatt gcatacaaaa
    24721 gctggacagc cagctttaga atatttgcaa agtcgtgggt tgtctttaga attaattgaa
    24781 gaattcaaca taggttttgc acctaatgag cgttcttttc ttcagcaagt attcaagaat
    24841 gaatcaattt caaatgaatt gttagaacgc tctggattgt ttgtcacgca tgatgatggc
    24901 cgtatgtctg atcgttttta tcaaagagtg atgtttccac ttcgtaattc tcaaggccag
    24961 acgatcggtt tttcaggacg ctggcttgca cctgaaaaag ggcaggaaaa agatcagccc
    25021 aaatacttga atagtccaga aacagaactg ttcaataaaa gacaagtttt atttaatctt
    25081 gataaagcaa gaagcgatat ccggaaaaac ggagaaattt tgttgttcga agggtttatg
    25141 gatgtgattg ctgcatggca ggctggtatc aaaactggat tagcttctat ggggacaagt
    25201 ctaacagctc aacagatcca acaaatggaa aagctgacga aagaattggt cttttgctat
    25261 gacggagaca acgcaggttt tgaagcaacc aatcgaggga ttgaattgct tcggcaaaac
    25321 agtcgattag cactttctgt cgtagtcgtt ccagaaaagc ttgatcctga tgaatatctc
    25381 cgtaagtatg gagaagcatc tttccaagaa ttagtggctc atgggcgcga aacagtcttt
    25441 acttttaaat cacgttattt aaaacaaggg aaaaatttag ataatgaaaa agacaagatc
    25501 gaatacctcg aagaattact agttgaactt agccgagttg tttcaccaat cgaacaagat
    25561 atgtatctat ctcaactgtc aacagagttt cagatcggca gagagacgat acaaaaacaa
    25621 ctgcgcgatc ttcgaaggac aaatcagcaa aatcgtcaag aacctcagga aagtcaacac
    25681 actcaggtta cgagacaaga aactgcacgg aaaaagagac ctttgacgca agtagaaaag
    25741 gcagagcaga ttctattata tcgtgcattt aaagaagttt ctgtccgtaa tattttaaaa
    25801 gaacgagaat ttcagtttat tcatgatgca tatgcagaag tttatttttt attcgatgca
    25861 tatgtctcac aacattatga attcaacctt gcagagtttt tagacttttt gcaagatgaa
    25921 aatttaaggc ggttggtggt ggaaatcgaa tatttaccgg tggcagaaga aagttcgcca
    25981 cgagaaatcc aagacttgct agatgtgatc agcaaatctg gcttagctga tgaaataaca
    26041 ttaaaaaaac aaatgcagca agaagccaga cgaactggaa acaaacagct tgagcttgaa
    26101 ttgacgattg aaatcatcaa tttaacgaaa cagttaaaac aagccaaata gctactcaag
    26161 gggggccttc ttttatggca aatggaacag ataaaaaaac gtatgaagca gcagtagcag
    26221 ctttcatcaa agaaaacaaa cccaaacgac aagttgtata tgatgattta tccaatcagc
    26281 ttgctacacc attcactttg aatgcagatg aaatggacaa attgatccaa aagattgaag
    26341 acgcaggtat cagtgtggtg gatgaaaatg gtgatccatc aattcacagc ttgaagagtg
    26401 ctgagaaaaa agctgaacaa gcaaaaacag aagatctttc tgcgccaacc ggtgtaaaaa
    26461 tcaatgaccc ggttcgtatg tatctgaaag aaattggccg tgtctccctt ttaactgcgg
    26521 aagaagaagt agcgttagca ttgaaaatcg aagaaggcga ccaagaagcg aaacagcgtc
    26581 tggcagaagc aaaccttcgt ttagtagtaa gtatcgctaa acgttatgta ggacgaggca
    26641 tgcagttcct tgacttgatc caagaaggaa acatgggact gatgaaggca gttgaaaaat
    26701 ttgactaccg caaaggattt aagttttcaa cctatgctac atggtggatt cgtcaagcca
    26761 tcactcgagc aatcgctgac caagcacgta cgatccgtat tccagttcat atggttgaaa
    26821 caattaataa attgatacgt atccaacgtc aattgctgca agaccttgga agagaaccta
    26881 cgccagaaga aatcggtgcg gaaatggatc ttccaacaga aaaagtccga gaaatcttga
    26941 aaatcgcgca agaacctgtt tcccttgaaa cgccaatcgg tgaagaagat gactctcact
    27001 taggtgattt catagaagac caggaagcaa caagtccagc agaacacgca gcttatgagt
    27061 tactaaaaga acaattggaa gatgtactag atacattgac tgatcgggaa gagaatgtcc
    27121 ttcgcttacg ttttggttta gatgatggcc ggacgagaac attggaagaa gtagggaaag
    27181 tgtttggtgt tactcgggaa cgtattcgtc agatcgaggc taaagcatta agaaaattgc
    27241 gccatccttc tcgttcaaaa caattaaaag atttcttaga ataagcgtgg actattgacc
    27301 agaagagaaa tcagtaaaac tgtttggttt attttaaaag aaaaaaagct tgagactacg
    27361 cagtctcaag cttttttttc tataggaagg aaattcattc attattttta agtaaatcct
    27421 aaaaataagc tattggcgtg acgtatagaa tatgatagat taaatggtag cgtaaaccta
    27481 gtaaaggagc cgatcgctaa gtgatgaaaa aatgtccaaa ttgcggatcg aaaaaaatgg
    27541 agcagaatac tatctgtcca gactgcggtc atcaaataac tgcagaagat cagcaaataa
    27601 aagaatcaat tgaagaaaca aatacagatg cagctatgca ggataaatca actgaagatg
    27661 cagtctttcc tgatacagaa ttaaacgatc caatcgaatg gtcggaattg aaagatttac
    27721 ctctagaatc tgtaatggaa ttattcgaaa aagcagaacc agaagacgaa gaaccacctg
    27781 ctcacgatca agtaactaat atagagaaaa atgaacagaa gaaagcaact gttcatgaga
    27841 cttcggaaga atcaggaaag aatcaagaag aaacgcttga atcaaaacaa aaaagaagag
    27901 tagctgaact gaaagaaatc gtcgataatg aggaagaaaa ttcgatacta tccgcttata
    27961 tcaaagcaca tcgtgaagat acaaaagaag agcacgcgaa agagctgctc aaaatgatca
    28021 gtgaaaaaat tgcacaggaa aacaaagtag agccagaaaa ggaacaagaa actgaaaaag
    28081 actctgatag agaaaacgcc acaggtaaag aaacagtaaa aataaaagac gtaaaaacgg
    28141 atatcacaga aaaaactgaa gtatttactg aaaaacaatt agaaaaggaa aaaacgaata
    28201 aactgccgga agcagagaaa aaagaaatac tcgaaaacac agacaaaaag tcaacagagc
    28261 ccgatgaaat aaatagtgaa tccgatgctg aaaatcaatc tgtaagcaaa gagggtactg
    28321 atcttgaagc aaaaatagaa aaagaagaga accagcctaa gctgtttgaa gcagaagcga
    28381 aatcaataac aatcgtgcca gatactgaaa aaacacatca agaatcaaac ttagaaaaaa
    28441 cagatccaga taaagcgcaa aagaaaaaat caaaaaaagt accttatctc gttttagcag
    28501 cagtacttct actcggtgga ggaagctggg cttattatga ccatcatcaa aaagtagaag
    28561 ccgaagtagc agcagaagca aaaagacagc aacaaatgga agaacttcat gagaaacttg
    28621 ctgcctttta tacagatgat caccatcaat ttattcgtgc agatatgatt catcaagatt
    28681 tatcaaaact aaaaaatgat ttatccagtc ttaagaatga aaaaggatat tcagagttag
    28741 aaacaactta ccaagatatc caatctaaga tccgagatat ccaaaaagtg aatgagtggt
    28801 tcatagcacc tgccatcgtc gatgatcatc tagcagatga catcaagttg aaagcagata
    28861 aagagatcca agcaatcgaa acggataaca cagattttgg aaaattgatt caacaagcac
    28921 aaacagaagc taaaaatcaa tatgctgaat tgcaaacagc taaagaaaaa acagctatag
    28981 tctttaaaga cggaaaagta gtggattcag caacacgcga ccaatacaag acggcaaaag
    29041 aagcagtaga aaaagtgaaa aatacagatc tatctgctca gttgaatgaa caattgaaac
    29101 aagtagataa gatgctcact gataaagaga aaaaagaaga agaggaaaag aaaaaagcag
    29161 aagaagcgaa aaaagcagct gaagaacaag caaaacaagc cgctcaagca gcagctgcag
    29221 ctcaagccgc tcaacaagct gcggtcgcac aagcagcaca gcaagaagcg gcatcctcga
    29281 ccccttcctc ttcaaactca gcgaatcagc caatcaaatc tgccagagca agtgatattg
    29341 ccgatgcatc taatccagca tggacgtggg caccaggtgt ccaaagcaaa gtgattgcta
    29401 catgtatcca acgtggatat atcgttgaag gaggttacag attagaaaaa gccagaatcg
    29461 aaaatgggga aggctattat aatctatatg ccacttcaac aaaatctgca ttgatgaaag
    29521 gcattggaga aagtgctttg ccattttaca tcgtaaccat caactgtaaa actggatggt
    29581 tcggaggaaa tgggagcaat taattttagg caaatgaaag aggctgtgag cctgccgttt
    29641 aacaccgttt attcgaaaaa ggggagtatg acacaactgt tgtcatactc cttttttcac
    29701 ctttccaaac caaggctcag ctattcaagt agataacatt atgttatgat agcaaagaaa
    29761 tgtattaaga aaacctttca ctatagaggg agaaaaaaat gaacgattta ttagcaacta
    29821 tatttacagg tattattatt gatgaaaatg aaaaaaacta ctttgtccaa aaaaacggac
    29881 aaacctttgg cttaagcaaa tcagaaggtg aacacaaaat cggtgaagca gttgaaggat
    29941 tcggttacca aaatcaaaaa catgaaaatc ggatcactac acaaattcct aaaagccgta
    30001 tcggtcatta cggttttgga atcgttactg ctacacgaaa agatttaggt gcgtttgtcg
    30061 atatcggtct tccagataaa gactttgtcg tatctcttga tgaattgccg acaatgacag
    30121 agctatggcc taaaaaaggg gatcgcttga tgatttccct tagagttgat gcaaaagacc
    30181 gtatttgggg cagtttagca gatgaaaaaa tattcaaatc tttaagcaaa tatggaacag
    30241 aagaaatgaa aaacaaagat gtgataggaa cagcataccg cttgaaatta acaggtacgt
    30301 acgttttaac ggaagatttc tatcttgggt ttatccatcc ttctgaacga tataatgaac
    30361 ctcgtctagg cgagcagata aaaggtcgag tgattggtgt ccgaccagac ggtgtactga
    30421 atctttcatt gaagccaaga gcatatgaag ctatatcaga tgatgcacaa atgattttaa
    30481 ccattttaga acgttctgca gatcagcaaa tcaactttac agataaatcg gatccagaag
    30541 aaatcatgaa agcttttggt atcagcaaag gagcatttaa gcgcgcatta ggtaatcttt
    30601 tgaaacaagg attgattaca caagaaaacg gtgtgacaaa actagtcaaa aaatctaatt
    30661 gagaatccct tgcatgtttt tcttaactag cttataataa ttttaattaa catagtgtta
    30721 tatctttttt tatttgtaat tattataaat tgttggggtt gagtaaatgg tcatgaatgc
    30781 tatttcggcg atgaaaaaaa caaaacagca gctacatgaa gcggggttta agctgacacc
    30841 gcaaagagaa gctactctgc ttgtgctttt ggaaaatgag aaagagcatt tatctgcaga
    30901 agaaatcttc tttttagtga aaaaaaagag tccggaaatt ggacttgcta ctgtttatcg
    30961 aacccttgaa atcttgacag aattgaaaat tgtcgataaa gtaagtttta atgatggatt
    31021 agcccgttat gatttgagaa aagaaggcgc caaacatttc catcatcatt tactatgctt
    31081 gaactgcggc acgatcgagg aagtagaaga ggatctttta cttgaagtgg agcaagtagt
    31141 agaagaacgt tatcattttt tagtaaaaga tcatcggcta acgttccatg gaatctgtca
    31201 ggaatgctat gaaaaagaaa aaagaaacca gtccgtctga gtgtctagac ggctttttct
    31261 ttgctcagat tatggaaaaa gactatcaga atgaagcggt atctgatatg atagagaggc
    31321 atgaggggga ttattatgaa agagcaaatc aacgattatc tccactactt gaatattgaa
    31381 cggggattgt ccgccaatac acgacaaagc tatgaacgtg atttggaaca gtacttgcat
    31441 tttctacagg agcagaaaat tgcttcatgg caggatgtcg atcgcttttt agtcatccgt
    31501 tttctggaga agttgcatga agaaaaaaaa gcgacagcaa ctgttactcg gatgatctca
    31561 agtctcagaa gattccatca gtttttacgg caagagcgta tcactgatca tgatccgatg
    31621 caacatatcg atacgccgaa gaaagcacag aaattaccaa gtacattgac tctgacagaa
    31681 gtagaaaagc tgattgaagc acctgatacg acaaaaacgt tagggataag agatcgtgca
    31741 atcttggaag tcatgtatgc tactgggatg cgcgtcagtg aattgattgg attgaaattg
    31801 ggagatcttc atctttctct tggactggta cagacagttg gaaaaggaga taaagagcgg
    31861 atcattcctt taggtgatta tgcgatccaa tggctggaac gctacttaga cgaagcacgt
    31921 ccattacttg taaaggacgc atctgaaatg catgtatttg tcaataatca tgggaaaggt
    31981 ctttctcggc aaggaatctg gaaaaatctg aagcagttag tacgagaggc tgggatttac
    32041 aaagaagtaa caccacatac gctgcggcat agctttgcca cacatctgct agaaaatgga
    32101 gctgatttgc ggacagtaca ggaattgtta ggtcacgcgg atatctctac gactcagatt
    32161 tatacacata tcacgaagaa aagaatgacg gatgtttata agcagcattt tccaagggct
    32221 tagtcatagg aaggtgagaa gatgttaaca cattaccgga aagaaaaccg taaaattgct
    32281 atgggactat tgagtttcca taagaaactg acaggtaaaa cttctttgtt gaaggagatt
    32341 gatacgtacg aatctgaaga cgattatgaa ctttttttct ttacgccaga aggttcaacc
    32401 aacattcaag ggattattgg tatttactgg gcaacgaacg atcagctgat catccatgac
    32461 atttctttga atccgtctta tcgcggtgag aatatgggct ttcaaatgtt agatgaatta
    32521 caggaaaagt acccggatgt ctccatccgt gcaactgaaa tcacagagcc ttacattgga
    32581 aaatggctag caaagaaaaa gaagaaagag tgaatgagtt gacggagatc aatattaaat
    32641 tagatgtatt cgagggtcct ctcgatctgc tgcttcatct gattcagcaa atggaaatcg
    32701 atatatatga tataccgatc gctacgatca cggagcaata tatgaattat atccatacaa
    32761 tgaaaacact cgagctagct gttgcaggag aatacttagt gatggctgct acattgatgg
    32821 caatcaaaag caagacattg cttcctgtcc aagaattcgt cacagacgat gaagaattat
    32881 gggaagaaga tcctcgagaa gaattggtca ctcagctttt agaataccgt aaatataaat
    32941 atgcagctga aagactgaca gaaaaagcag aggaacgaag tctttattac acaaaagaac
    33001 ctatgaacat cgatgggtta gcggatgctg aaaagccgtt gaagcgagga caagtcaaaa
    33061 aaatcgattt gatccgtgcc atgcagaaaa tcttagaacg taagaaattt tctcaaaaag
    33121 tggaaaaaac gatcacgccc gatgacacaa cgatcgaaga acgaatggta tttatcgaag
    33181 agcgtataaa aagaaatgcg catggctgta ctttcgattc tttttttgag tcaccttcta
    33241 aaagtgaagt tgtcactact tttatggctt tgctagaatt gatgaaaaaa gggcagatca
    33301 ttgcgcaaca aaaagaaaac tatgaatcaa ttatgcttta tccagcagta ggagataccg
    33361 aacatgacga aattaagtga acttgaagcc cttcttttta ttgttggcga ggaagggatc
    33421 agtaatgaag aattaagcgg actgttaaat ctcagcgaag cagaagtcac aagtttagtg
    33481 aaagaattaa aagaatctta cactcaaaac tcgcactcag ctttgcatat ttttgaaacg
    33541 gaaagccatt atgtattaac gactaaaaaa gagctggctc ccttacttaa gcgatatgcg
    33601 caagggacat caaacacatt atctcaagcc gccgtcgaaa ccttagcaat catcgcttat
    33661 aaacagccaa tcacaagagc tgaaatcgag cagattcgag gagttcaggt ttcaggagcg
    33721 gtacagcgtc tagctgccca taaactgatt gaagaaaaag gtagagtaga aggaccaggt
    33781 cggccgatat tatatggaac aactggctac ttcttagatt attttggttt gaaatcatta
    33841 gaagaactgc ctgatacaca agaaatggaa atttcacttg aagaagacgt gtcaccagat
    33901 ctattctttg atggaatgaa taaaggagaa taattgtaat ggagagattg cagaaagtaa
    33961 ttgcccacgc tggcatcact tctcgtcgaa aagcagaaga attcatcctt gcaggacgtg
    34021 taaaagtaaa cggacaagtt gtaagggaat tagggataaa agttgggaaa catgacactg
    34081 tggaagtgga tgaagtccca atttatcaag aagaatacgg ttactattta ttgtacaaac
    34141 ctaaaggagt tatttctgct gttgcagatg acaaaggaag aaaagttgtt acggatcttt
    34201 tgccgatggt aaaagaacgt atctatcctg taggtcgact ggattacgat acttcgggga
    34261 tcttgctact gacaaatgat ggagattttg ctcagcgcct gacacatcca aaacatgaag
    34321 tggataaagt ttacgttgca aaggtaaaag ggatcgctac aaaatcgatg cttgcacctt
    34381 tgacaaaagg aataaagatc gaaggacgaa aaacatctcc agctaaattc caaattttat
    34441 cagtggatcc taagacaaac cacagtatcg tggaattgac gatccacgaa ggacgtaatc
    34501 atcaagtgaa aaatatgctt caagcagttg gtcttcctgt gcaaaaacta aaacgtgaaa
    34561 aatacggaga tctgacttta caaggcctac gtcctgggga ttaccgtgaa ctgaacaaaa
    34621 aagaagtcag cagtttgctg aaccactcaa aatgaacact tactttttat aagtgttaga
    34681 tgttgcgttt tttgaataga tatgtataat gaatatgtaa acaaaaatta tataaggttc
    34741 gtcttcaggg gcagggtgaa attcccgacc ggtggttata gtccacgacc tattttgcaa
    34801 aaaaatagct gaatcggtga aattccgata ccgacagtat agtctggata aagaagatag
    34861 agcttatttg acgacgcttg atcaaataac tctaactcta ttttccctgg aaaatagagt
    34921 tttttcattt ggagagaaac gtcaaaaagt tcgctgaaga ggagtcctaa caggaggatt
    34981 tcaaatgaag aacacccgtg tacagaaaat ggttgcagta gcgatgtttg ctgcgatcgc
    35041 attagtttta cagtattttg cttttccgat catgccggct tttagttttt tgaagatcga
    35101 ttttagtgat atctcggtat tgatcagcat gttcttattc ggtccagcgg ccggagttgc
    35161 aacagcgttc ttgcgttcag tattacatct gctgactaca ggtttttctc cggataattt
    35221 ggtaggagac actgctagtt tcttatcaac aacgatcttt acattcccga tttattattt
    35281 ctttagtgta aagaaacagc ataaaggaag aaacaaattt ttaggtgtca ttacaggaac
    35341 catcgctcta actattttta tgagtatcgc aaattatttc gttattacgc ctctttactt
    35401 aatgttttta gggctgaacg caacacagat gctgggcatg tctttagcaa actatgtact
    35461 gattgggatc gttcctttca acttgatcaa aggattcatc gtgagtgctg tctttttagt
    35521 gctgcatgcg caacttcttc catggttaag tcgaaaacaa catcagttcg aacaacgtca
    35581 tacagcagtc aaataaatat caaaaagatc aagaaatttc ttggtctttt tttttggaaa
    35641 aaaatacaag acatgcaata atgtaaacgt tttatttgtt tgttttgagt atttttttga
    35701 tttatgcata aaaatcaatc aattctttgc aaatgtgaaa ttcttctcct tttaaaagac
    35761 gaaaatgttt gtttataaaa aaaaggaaac gctacaatga aaaagaagaa agctttaaaa
    35821 acctgaaaaa agaggaggga atagggaaaa acgaactttt tgaacaaaag ttttacaaaa
    35881 tgcgtgttat tatttttgtt ctgatactct ttttgataaa ataacattag agccaaaatt
    35941 gttgtctcta acggaaaaga aaggaagaag aagatggaac aaacaaaaaa gaaaaaattt
    36001 caaatgcctt cagcgtatac ggtcttattt attattattg cagtaatcgc tgtacttact
    36061 tggtttatac cagctgggca ctatagtgta gacgatgcag gcaacatcgt cgctggatca
    36121 tatgaacgag tacaacaaaa tcctcaaggg ctgtgggata ttttcatggc gccaatcaat
    36181 gggatgctag gtgtaccagc gggggagcta actaaagaaa cacctgctgc aatcccaatc
    36241 tctttcttca ttttggttgt tggtggattt ttaggtatta tcaacaaaac aggggcacta
    36301 gatgccggga ttgcttcagt tgtgaagaaa ttcaaaggaa aagaaaaaat gcttattcca
    36361 gtattgatgc tgttatttgc attaggcggc tctacttacg gaatggcaga agaaacgatt
    36421 ccattctatg ctttactgat cccagtcatg atggctgtag gctttgatac agtagtagct
    36481 gtagcaatcg tattagtagg gtctcaagta ggctgtctag cttcaacggt aaatcctttt
    36541 gcaacaggcg tagcttcaca agctgttggg atttcgatgg gtgaaggtat tggtcttcga
    36601 ttcttgatgt tcgttatttt gttagctatt tctattgtat atgtgtatcg ttatgcatca
    36661 aagatcgaaa aagatccaac aaaatcatta gtatacaacc aacgtaaaga agatatgaag
    36721 catttcgata ttgaagcaat cggtcgtcaa gaagtaatca cgaaaaaaca aaaacatgtt
    36781 aatgtattgt tctttattac ttttggtatc atgatcatca gtttgattcc ttggacaacc
    36841 ttgaatgcga atttcacgat ttttgaatca ttgacaaact ggttaacaaa ccttccggta
    36901 ctaggtacgg ttctaggtaa aagcatgctt ccattaggtg actggtactt cccagaaatt
    36961 actatgttat tcttagtaat gtccatcgta gtagcaatca cttatggcat gaaagagtct
    37021 gaatttgtct ctgaattttt agctggtgct tctgatttga tcggtgtagc aatcgtagta
    37081 gctgttgctc gtggtatcca agtggttatg aacaatggat taatcacaga caccatctta
    37141 cattggggcg aacaaggatt aagttcatta tcttcaagtg tattcatcat tattacattt
    37201 attttctata tccctatgtc attcttgatt ccttcaactt ccggcttagc tgctgcaaca
    37261 atgggaatca tgggacctat gggtgaattt gccggcgtcg gtaaggattt agtaatcact
    37321 gcttatcaat ctgcatctgg tttagtaaac ttaattacac caacatctgc tatcgtaatg
    37381 ggtgctgtag caattgctcg tttcgatgtt tcagtgtggt ggaaatttac cggtaaatta
    37441 attgcgatat tatttgtagc catctgtgtg atcctaggtg tagcagcaat gttctaaacc
    37501 tagacaaata caggtacaag gtctatcata agaatgaaga aaccgataaa cggctatcga
    37561 taagcacgtg gataagtcaa tagctccatt cagtgagtgt tttttctttc atcatttgtt
    37621 aagaacattc tgcttgctga tggagcaggc ttaaaacgtc attatttgag aagaagaggt
    37681 tgtgtcggag gaatttgaca tgaccctttt taacatatta ggaggataat atcatgaaaa
    37741 aatttgtgac agaacaacat catgaacaag cattagaatc attgagcgaa ttgatccgaa
    37801 ttccttccgt actagatgaa gcagattcag gacaaggaca tcccttcggt aaaaaagtga
    37861 ttgaagcttt ggataaagta ttggaaatca gtgaaaagct aggatttaga acatttaaag
    37921 atccagaagg ctattacggt tattcagaga ttggctctgg agatgaatta ttcggtatct
    37981 tgtgtcatat ggacgttgtt ccagcagggg acgaaaataa ttgggaaaca aaaccattcg
    38041 atccaactat aaaagacggc tggttagttg gtcgcggatc ccaagatgac aaaggtcctt
    38101 ctattgcggc aatgtatgcc gtcaaagctt tgattgatgc tggcgtggaa ttcaagacac
    38161 gtattcgttt tatctttggt acagatgaag aaaatttatg gcgttgttta gaaaaatata
    38221 acgaaaaaga agaaggaatt acgcaaggat ttgcgccaga tgctgaattt cctttaattt
    38281 atgcagaaaa aggcttattg caagcttact tgacaggacc aggaacgaac gagttttctg
    38341 taaaagctgg aggagcgttg aatgttgttc ctgatgcagc tccttactct ggagaaaagc
    38401 tttctgaagt aaaagaagca ttgaaaaaac acgaattcga tttcgaagaa caaggtgaag
    38461 gcatcgtcgt tcttggtaaa agtatccacg cgaaagatgc agcacaagga gtgaatgcta
    38521 tttctcgtct ggcaattgct ctttcagaag tatttgattt tggcccaatc aatttcttag
    38581 gaaaattagt tcaagaaaat gcaactggtg aagctgtcgt gggcaaaaca gaagatgaac
    38641 aatccggcga gctaacgatg aactttgcaa gtttggaaat cacaccagaa caaacaaaaa
    38701 tcggtgtaga tatgagaatc cctgttacat tcaagaaaga tgatttagta gctaaattaa
    38761 ctaaaacagc agaaaaatat gggttgactt atgaagaatt tgacttcctt gattcattat
    38821 acgtgccact agacagtgag ttagtgaaaa atctattggg gacatatcgc gatattactg
    38881 gagacatgac tgaaccgttt gtatcaggcg gcgctacttt tgctcgtacg atgaaccagt
    38941 gtgtggcatt cggtgcgatg ttccctgata ctcctgactt tatgcaccag gcgaatgaac
    39001 gatgggaatt aagtagtatg tataaagcaa tggaaattta tgcagaagct gtgtatcgtt
    39061 tatgtgctaa ataaaaataa acatacaaaa agggcggata ttcaaatgga tattccgtct
    39121 ttttttattt gatgctggaa agtttgcatt tctctcttat ttttttaagg tataatcata
    39181 cttgattaag aaaaaggggt cactcagata gaaaaggtgg gagaaagtga aaatatttag
    39241 acgaatacta attggcttag tcatcatttt aggattactc ggtggagccg gttattacta
    39301 tatacagcaa aatacgtacg aaccatcgtc agaagcgcaa gtgttggtaa aagaaggaaa
    39361 aaacaaagaa gattacttat tttttgaagg agagaaaaag aatcatggaa agtctccatt
    39421 acttattttt tatcctggtg ctctcgtaga gccagaaagt tatagtattt gggcgcaaga
    39481 tttagcaaaa aacggctttt cagtcgctat tgtcaaaatg ccacttgatc tagcagtgat
    39541 gggcggaaac aaagcggata aggtcaaaga agattttcca aattccgatt atgtgatcgg
    39601 tggccattca ttaggtggcg taatggcaag tcgttatgtc aataagaatc attcagacaa
    39661 acatttaaaa ggggtattct tcatggcaag ttatcccgac aaaaaaggaa cattgacaga
    39721 atcagctgtt cctgttttat cgatcacggc gacaaaagat aaagtattga atcaggaagc
    39781 ttatcaagaa gctaagcagt atttaccgga aagaacagag tatgtatcaa tcaatggcgg
    39841 aaatcatgga ggttttggca gctatggtga gcaaaaagga gatggagagg cagaaatttc
    39901 taacgcagac cagcaaactg tgatcgtcaa tacattggaa aactggctga aaaagttgaa
    39961 ataatataaa tgggtcctat agggtccgtg aaaaaactag tcaattaact atagatagca
    40021 aatgaatctt tcaaaataag tcgggatttc acggaacaag agaaacagat cccggcttat
    40081 tttcaagttt agagaactta gtgtacgttt ttaaagtttc gtttatattt atattttatt
    40141 ttctaataaa atcgctcgaa ctggacattt ttgatacgct ttttttacat atgtttcttc
    40201 ttccggcatc acgaactgtt gatgagcctc tggttcttgg gcaaataaaa cgattccttc
    40261 ttcatcataa tcgaaaatat ctggcgcttc gatttgacaa agaccgcatg caatacagcg
    40321 ttcaggtaca agtttacata gtagtgacat ttctaaccat cctaacttaa ggagaataat
    40381 atgattgttt ttattttatc gctattttca tcaggacaca agctgcggat tagttctctt
    40441 taccaactcc ttgtgggaaa acgaacgact tcagtactta tttatggatt tacccacgaa
    40501 cttttgttta tccataacag ttttccagaa ttgaagcagg ataagtttta tcaaattttg
    40561 caaaaaattg cgcagcaagg ttggatcgaa ataaatgaaa atgaagcaaa gctaacgcct
    40621 gcaggggctg atatgttgtc cgaacatcaa atagaacata ctggtttgcg ttttgaccgc
    40681 tatggcagaa caggggaaac tagttggcgg ctcattaaat ttgctgttca agtaatttct
    40741 aatctggcaa cgggtattca agactatctt ccagcagaga caagtccttt ttatactttc
    40801 caactaaaaa aatggcttag tgaaagccaa ttgccacgtg aaattttgat tgatactgcg
    40861 tatgagagtt tggtgcagct gttttcggag ataccagaag aagcagcaga ttttttagct
    40921 aaccaattat ctggaaatga gcgaacaggg ctgcttcctt atcagctggc aaaaaacaat
    40981 gatgagtcag cggtttatct gcaacaaagc agatgtatcc accttttgtt agcacaaatc
    41041 gaaaaacggc cagattcgct ttggcacgct ttgatcgatt cattattaca gcaaaatttc
    41101 aatcaaagca tgatgatcac caagcagatg tttatgaatg gtcagacaat tgaccagatc
    41161 atggcaatcc gtcatttaaa aagaggaaca gtaacagatc atcttattga gtgggccttg
    41221 ttttttgatg attttccata cgagtggatt ctctctgcag aaacaatcga acgtttggaa
    41281 ccaaataagg attccgtgcg agagtggcgc tttagcgaat ggaatgtaga tggacaatta
    41341 gattatggag aatttcgttt gtatcaaatc tatctattga gaaaggaagc gatacagaat
    41401 gttaataaat gaattaaaaa aatattttgg atttgattca tttcgtcccg gccaagaaga
    41461 aattattcag gcgttgcttg atggagaaga tactctcgct attttgccta caggaacggg
    41521 aaaaagcctt tgctatcaat tgacggggta tttaatggaa ggtctggtca tcatcgtgtc
    41581 accgctgctt tctctaatgg aagatcaagt gacgcaatta caaaaacgag gggagaaacg
    41641 agtagctgct ttcaatagtt tactttcgca atctgaaaga agatacgtcc tccgtcatct
    41701 atcgcaatat aaatttttgt ttttgagtcc cgaaatgttg actaatcctt ctcttttaga
    41761 gcatctgaaa aaacaaaata ttgcgttata tgtagtagat gaggctcatt gcgtgtctca
    41821 atggggagtt gatttccgtc cagaatatca acagttagga aaaatccgaa agcttttagg
    41881 aaacccggtc acactggcac taacggcgac agcaacagat ttagtagcaa gagatatacg
    41941 tcatgtacta tttgatcgac caccaaaaga gatcagacaa tcagtcaatc ggcaaaatat
    42001 ctcgcttttt gttcggaaaa cgcagcaaaa agaacaagaa cttgaacact tcatggaaag
    42061 agcacaagga gcggcaatca tttattgtgc aacaaaaaaa gaagtcgaac gattgtatca
    42121 tttattcagg gaacgtttta cagttggata ctatcacggt ggtcttgatg cagctcaaag
    42181 aagacaactt caacagcagt ttgtaaaaaa tcagctgcag tttctcattg ctacgaatgc
    42241 atttggtatg gggatcgata aatctgatat ccgttatgtg gtccattatg atttgccaga
    42301 cagtctagaa aactatgtac aagaaatcgg tcgagcaggc agagatcagg aagaaagtgc
    42361 agcaattctt ttttatcaaa gtggggatga acgtatccat tatttcttta atcaactttc
    42421 tagagaacag cgacaaagtt ttgagcttta tttggaatat gccacagagc aagctccctt
    42481 tgatgagctg caaaaaaaat ggatggagct gatccagcag tctgaaaaac cagaaaattg
    42541 ggtggaacgg ctcaaacgtc aggaaaaaga aaaagaattc cgcctgcagc agatgcttcg
    42601 ctatattaat gaagaaaact gccgcagaaa attcatctta gcttattttg gcgaaaaact
    42661 ctccgaaaag ccgcagaatt gttgtgatat tgatggagca aaagcagaac ttctgccaaa
    42721 gaggaagctt ttttctaaga ctgaacaact tcattgggaa acgattttac taaatttatt
    42781 taaaaaaaac atggaacatt gattttttag gacaagacac tttttcctat ggtataatac
    42841 acacgataga gtttaggagg taaaacagtg agcagaaaag atagacatca atctacagaa
    42901 aataacgaaa cacaagaacc atgggaacaa ccaatctacg atacagatga tgaagtttca
    42961 tcaagaagtt ctcagagaca acaaaagaaa ggaaatacat tatttctttc gctatttttg
    43021 attttactgg tcttatgtat cgctattcca gcaggtgctt atttctggat cagaaatgga
    43081 tcaagcaaca atgcaacagc agcaacttct tctgaaactt cgacatcagt agtagaaagt
    43141 tctacaagta caacaaaaga ggagtcaact gagacttcaa cagtagaatc tagtacaatc
    43201 gcggaagaat ctactgtgtc tcaagataca ggtgtaacgt cagaaagcag tattgcagca
    43261 gagacaacac cgacttcctc aagtgaggca acttatcaag caggggattc aagcagcaat
    43321 gcagcagaaa caacaccttc ttcatcagct gcagccggtt caacaacgac tgttcaagca
    43381 ggcgaaggtc caaaacaagt ggctgagcgt gcagggatca ccactgatca attgtttgaa
    43441 ttgaatggct tagatccaaa caactttatg ctttaccctg gtcaagaatt aagaattaaa
    43501 taataatagc cgcgctttgc ggcttttttt ctgagaaaaa ggagttatat catgacacaa
    43561 atcagtattg caatcgacgg gcctgcttca tctgggaaaa gtaccgttgc caagatatta
    43621 gcaaaagaat tgaattatat ttatacagat accggtgcca tgtatcgcgc agtaacttat
    43681 ttagctatta cgaacaatgt atctttttca gatgaagaag ggctagtttc gctgatcaaa
    43741 aagtatccta tctctttttc gcaacaagaa gatggacagc atgtattcat agcatcaaaa
    43801 gatgtgactt tagaaatccg tcaaccagat gtcacaaaag ctgtttctga agtctctgca
    43861 catggaaaag tcagagaaga attagttgct gtccaacgaa aaatagggga acgcggtggc
    43921 gttgtcatgg acggaagaga catcgggaca gctgtattac cgaatgcaga agtgaaaatc
    43981 tttttagtag caagcgtaag tgagcgtgca gaaagacgtt tcaaagagaa ccaagaaaaa
    44041 ggaattccga ctgatcttga aacactgacg aaagagatac aggaaagaga ccattatgat
    44101 tcaacaaggg acgtctctcc gctgaagcaa gcagaggatg ctgttcgtat cgatacgact
    44161 ggcaaatcga ttccagaagt cgttgcagca ataaaagctg ttgttttgaa aaaaggttat
    44221 cttctttttt aaattaagaa aaaaaatgaa aaacaattgt tttttcaagg aaaacgaacg
    44281 aaatcgcttt attttttttg aataatcaat taaactaaaa acagtaccgt acttacggca
    44341 gaatgttggt taggaggaca agtgttatga cagaatttga tcaaaatcaa gagaacaacc
    44401 agtcaatgga agacgcaatg aaaagcgtac aagaagtaaa agttggcgat gttgttaagg
    44461 gcgaagtttt agtcattgaa gacaaacaag tgattgtcgg aatcgaaggg gctggagttg
    44521 aaggagtcgt tccggcaaaa gaattatcaa ctttgccggt agaagatatc agcgaagtag
    44581 taaaagtcgg tgatgtatta gatttagtcg ttatcagtac gatcggcaaa gataaagaaa
    44641 acggcagcta tctactttca aaacgccgtt tagatgctaa aaaagtttgg gaagatatcg
    44701 aaagagattt ccaagagggc aaggtcatcg aagcacctgt tacaaatgtt gtcaaaggcg
    44761 gtttagtcgt tgacgtcggt gtacgtggtt ttgtccctgc atcaatggtg gaagatcatt
    44821 ttgttgcaga cttttcagac tataaaggca aaacattgag ctttaaaatc atcgaaatcg
    44881 aaccatcaga aaatcgtttg atcttatcgc ataaagcggt agtggaaaac gaaagagaag
    44941 aaaagaaaaa agaaattctt tcaactatcc atgagggaga tgttctcagt ggacgtgttg
    45001 cacgtcttac tgattttggc gcatttgtcg acctaggcgg aattgacggt ctagtccatg
    45061 tctcagagat ttcacatagt catgtagcaa aaccaagtga tgtattggct gtcaatgatg
    45121 atgtaaatgt caaagtgttg tctatcaatc ctgaacaaga acgtgtttcc ttgtcaatca
    45181 aagacacaca gcctggacct tggtcagaaa tcgatacaaa agctccagtt ggcacagtat
    45241 tagacggaac agttaaacgt ttgacaagct tcggtgcctt tgttgaagtg ttccctggag
    45301 tagaagggct agtccatatc tctcaaattt cacataaaca tattgcgaca ccacatgaag
    45361 tgcttcatga aggtgacgaa gtgaaagtga aagtattaga gatccatcca gaagaacatc
    45421 gtattgcatt gagtatcaaa gctttagaag aaaaaccagc ttcagaagaa gaaccaaaaa
    45481 atgttgagtc ttatgaatta ccagaagaaa atactggatt tactatggga gacattttag
    45541 gtgatgcgtt gaaagaagaa gtagaagaaa aagcaaccga tgattctgaa aaataattga
    45601 ttttgtatct gtgactgaga tgaaagcagc tgcttttgtc tcagtttttc ttttgaaaag
    45661 cgattagtat ttcaataagg aataaaaata cttcgtttaa ttgacaatct atgcttgaaa
    45721 gttggcaatg cattcgttaa actaagtgag attggaaact tgtgaggagg aaataatatg
    45781 gcaaatccaa caattgcaat cgttggccgc ccgaacgtcg gtaagtctac gatttttaac
    45841 cgcatcgctg gtgagcgtat ctcgattgtc gaagatactc ctggagtaac tcgtgatcgt
    45901 atttatgcaa aaggtgaatg gttaggtcga gaattcagcg tgatcgacac aggcggaatt
    45961 gatcttggcg atgaaccttt tatggatcag atcaagcatc aagcagaaat cgcgattgaa
    46021 gaagcagatg taatcatttg tgtagtcagc ggacgcgaag gcgtgacaga cgcagatgag
    46081 atggttgcga aaattttata cagaagtaat aaaccagtga ttttagcagt caataaagta
    46141 gataatccag aaatgagaaa cgatatctat gaattttatt ccttagggct aggcgatcct
    46201 tatcctgttt caggaagcca tggactaggg attggtgata ttttagatga agcagtgaag
    46261 tatttctctg aagagactga agaagaggac gacgacacga ttaagtttag tttgattggt
    46321 cgcccaaatg ttggtaaatc ctctttgatc aatgcgattt taggagaaga acgtgtaatt
    46381 gtttcggata ttgaaggaac gacccgagac gcaatcgaca cccacttcgt ttctgaaagt
    46441 ggtcagaaat tcttaatgat agatacagct ggtatgcgta aacgcggaaa agtatacgaa
    46501 aatactgaaa aatacagcgt gatgcgtgca atgcgtgcca ttgaccgttc tgatatcgtt
    46561 ttgatggtac tgaatgctga agaagggatt cgggaacaag acaagcgagt agcgggttac
    46621 gcccatgaag ctggtcgtgg aattattatc gtcgtgaata aatgggatct tgtaaagaaa
    46681 gaaaccaata caatgcgtga tttcgaacaa gagattcgtg atgaattccg gtatcttgat
    46741 tacgctccaa tcgtttttgt ttctgcagtg actaaacaac ggttggaacg tctgcctgaa
    46801 atgatcgaac aagtaagtat gaaccagaat cttcgtatct cttcggctgt gttgaatgat
    46861 atcattatgg atgctgtagc aatcaatcca acaccaacag ataaaggaaa acgcctgaag
    46921 attttttatg ctacacaagt cgctgtcaaa ccacctacat ttgtcgtttt tgtcaatgaa
    46981 gaggaattga tgcatttttc ttatgaacgt ttcttagaaa atcaaatcag aaaagcattt
    47041 acttttgaag ggacgtccat ccgaattatc cctcgtagga gaaaataggc attttaccat
    47101 atcgacgttt ttttttcaaa agaaaaagac agtttcgttt gttacgtttc tatgactaaa
    47161 aaaagcatgt gaaaattggt aaaaaccttg ctattaagcg gttcatatga tatcgtgata
    47221 acagaatatt gaagttaatc aatatttctt tttaaacttc tggaccactc agaaagtttt
    47281 aactaaatga tatttctatc atatctcttg tggaggaggt gaatctatcc atggcaaaca
    47341 aagcagaatt aatcgaaaaa gttgcttcag ctactgattt aactaaaaaa gatgcaactg
    47401 cagcagtaga tgctgtattt tcaactattc aagatgcttt agctaacggc gaaaaagttc
    47461 aattaatcgg ttttggtaac tttgaagttc gcgaacgcgc agctcgcaaa ggtcgtaacc
    47521 cacaaactgg tgaagaaatc gaaattccag ctagcaaagt acctgcattc aaaccaggta
    47581 aagcattaaa agacgctgtt aaataagaca gttgaaaaag cgcttgtggc tctagggcta
    47641 caagtgcttt tctttttatt tcctagcaga aaaggaataa ataagagaca gagattccga
    47701 atccgtatgt catacataga aaacacatgt attttattta atttttattt atttgcaagt
    47761 tatgaggtga gctttgctct tatcttttct agaatttgct tactaatggt aaaataaaaa
    47821 taagaagagg agggatgcgt atggaacttg attatcaagt agaagatgcg ttgacgaaag
    47881 tgttttacgg agaaacgcta gtcagtcagc taagagaact ttccattgat cagccaatct
    47941 tatttttaac gaatcagcgc tattatgatt tgtttgcaga taagatcaac ccgttatttg
    48001 caaatcaagc aaacaatgac tggtatattt gtgccaacac tcaatgcaat catttaacag
    48061 aattaaagaa cctgcttgat ttcgtcaaac gctatcccga aaatcaatcc atgtttatca
    48121 tcggatttgg aaatgaaggg atcatggatc tggcaggttt ttttcaaaaa catacaaccc
    48181 ttgattcatc actatggatg atccctgtat cgatccgttc tctaagccga gcattaacac
    48241 cgcagcggac gattttgcag ctaccaaatc gtgtagtgct gcaaaccatc aatctgccag
    48301 aacgcatcgt ttatgatcaa acaatcagcg agcggcaggt ggacggaaag cagattgact
    48361 ttttgacttt tatctgttgc ggaatgattt gtgatcatcg ttttttgcaa aatctgtata
    48421 aaaactatcc aaataaaaag caattaacga ctcggccgtt tgccgggatg atcgaagaaa
    48481 tgatctattt ttatcaatta gaggcagaaa acctgtcaaa ttatggtaaa ctgtttgaac
    48541 aagcttttta tctgaccgaa aatggccatc tactttctgc tagtatgaaa aaatttttag
    48601 gaatgatgtt tcatttcgtg tggaatctcc aagcatatcc ttttgatttc caactaaaaa
    48661 acttttttgt gtggctggac ttgcttggct atccaatcca ctggctggaa cagatttcaa
    48721 agatggagta tttggaaaaa gttctttttt tggcagaaaa aagcaaaaaa atacttgttt
    48781 tagaaaaaat aggtataata gacggatacc aacatccaaa cgaaaaagaa ttattgagaa
    48841 caatgacggt atttcaacaa atcgtcagtg agataagagg gaattgaaat gaatacaaat
    48901 agcgaaaaaa tgttgcaagc tttatcagat gaagacttgt cacaagcaca aatcttttta
    48961 gaaaaagcat taaaagaaga cccagcagat atattagctg aactaggcga agaattgctg
    49021 gctattggct ttttgatcga agcaaaacag atttttgaac aactgatcaa ccaataccca
    49081 gaaaatgacg gactgaacat cccgttagca gaaattgcaa tcgaagataa tgagatcgac
    49141 ttagcttttg aatacctgga aaaagtggat aagcaaagtg attactatcc gcaaagctta
    49201 ttggcgatag ctgatttgta ccaagttatc ggaatccctg aagtgagtga agcaaaattg
    49261 aaagaagcag ctgggttgct tcctgatgaa ccattgatcc agtttgcgct agctgaattg
    49321 tatttttcag ttgatcgttt caaagaggca gcagtggttt atgaaacatt gttggcaagc
    49381 gggataaatg aaatttcagg aatctcgatg caggaacgtt taggacaatc cttaagtatg
    49441 caaggagaat tcgaagcagc tatcgtacct ttagaagaag cactgaaaga agaaaggaca
    49501 gatgatcgac tgttccagct agcttttacg aatcttcaat tgaaagaaaa tcagcaggca
    49561 atcaactatc tggaagaact tcgagaagtt aatccgcatt atcaatcgtt atatctatat
    49621 ctaggacaag ctcttcaaga agaagaaatg attgaagaag cacaaacagt gttagaagaa
    49681 ggaatcaaag aaaatcctta ccaagtcgaa ctttatcacc tggcctcgga aaatgctttc
    49741 cgtctgcatg atagacagcg agcagaagaa cttttactga aagctttaga agtaggagac
    49801 aaacaagatg aaacattact tactttaagt aatctttact tggatgatga aagatatgaa
    49861 gatgtgatac aagtgatcaa tcagatggaa gaaactgcta atccttatgc agaatggaac
    49921 ctagcccatg cttataatga attagaagac tttaccgttg cagctgtaca ctatgaacag
    49981 gcttatcatg aactatcaca tgaaccagat ttcttgaaag agtatgcctt tttcttaaga
    50041 gaagaagggc agctgaaaag agcgcaagaa ttattgactt attatttaac acttgaacca
    50101 ggagatatgg aggcactgtc attattagat gacctaacag agaggtgaga aaaatgctaa
    50161 ttgatgtacg ggaaaagaag aattttttaa cgtggatggt caatcatgta tcttttagcc
    50221 gaagagaagt tttttggatc ttgaattatc ttgccaatca tgaagcgatt ttaagcaatg
    50281 tccactttgt tgaaggggcg aattttgcgg atcgaggact gcagatcagt gatctatctg
    50341 ttgaagggga acccatgaaa ttgtttttgc agggaaaaga atttacggac acagaccaga
    50401 tttttcatga aatccgtttg aattggaaaa agcctttata tgtggaatgt atattcgaaa
    50461 atgcttggca gacaagagaa tatctatcta tcttagaaga caatccattt tcttcttggg
    50521 attcttcagt cagtgaagaa gatactgaat taattgaacg atattttcaa gaaaaagaag
    50581 aagaggcaaa actaaaaatg ctttatgctc aaatcgatca agcactagaa gatggagata
    50641 gggatgcttt tttgaaatta tctgatgaag ttaaccgaaa gatccttttg cagacacaaa
    50701 agaaaagtaa ccgggtaagt gaacataaca attagttaaa aatagtatag tactaaaaaa
    50761 gacaaaggtc agctggatgt ggaatctttc ctcatagcct atctttgtct tttttcttat
    50821 agttttttca gtaatcttgt ctgtggcttc aaataagtaa agccttgccc ttccacttca
    50881 tgcacattga tgattgaaag gaaagctttt tcgtcaatct cgtggacgat cctcttagct
    50941 tcagttaatt cactagagct aacgaccata tagatgattt tttttgaatt accagagaat
    51001 gcgccttctc cagaaaggaa agtcacacct ctttgcagac cggtcatcag tagtggtgca
    51061 atctcttcgc ttttgttgga gacgacaagt agaccttttg cagaatatcc gccatctaag
    51121 atcgaatcaa tcacacgact gaaaacatac gaaacaatca acgtatacat catacgtttc
    51181 aagtcgatat aggttaatga agcaagtaag acgatgatat caaatgccaa tagggaacgc
    51241 cccatagtga tgccgtaatt ttgttctaaa atccgagcga taatatcact gccgcctgtt
    51301 gttccaccca tgcggtagac gatcccgctt cctacacctg caactaaacc cgcaagtaga
    51361 gaaacgatca gtagatcatg ctgtaaattg atttctagtg gaaccctttg ccagatccat
    51421 aaaaagaatg acaaagaaag tgtacctaga acagtatagg ccaaggaacg tttaccgagg
    51481 atctttcctc ctagtaaaat caatgggatg ttgattaata aagtcgtata agctggatca
    51541 ataccaaata aagctcttaa gatcaaggtg atccctgaga ctcccccttc tgctaaatga
    51601 ttagcgatgt tcaagtaaac aagaccaaat gcataaatac ttgttcctat aatgataaaa
    51661 agaacttctt ttactgtttt tcgtttgacc atatgaattc cagctttcaa ttatgtattt
    51721 tcacagaatt tccatttaga aattccttcc taaatgtagc atggaaattc gattttctca
    51781 attgctctta tctaggattt gcgttaaagt tttggtaaga tagttttgga gggaaaaaga
    51841 tggataaaaa gcgttcttta tacagtatgc agcaggaagt ggatgaatat atccagcaat
    51901 tcaaggtggg gtatttctct ccattagctc agatggctcg attaactgaa gaagtaggtg
    51961 aattggcaag agaagtcaac cacacttatg gagaaaaaag caaaaaggct tctgaaccag
    52021 ttaactcggt agcagaagaa cttggtgatg ttttattcgt tacgatgatc atggctaatt
    52081 cattgaatat cgacttaact gaagtttttg aaaaaaacat ggaaaaattc aatcaaagag
    52141 accataatcg ctttgagcgt aaggacgagt aaacaagatg aagtggtcct ggccttgaag
    52201 actctgaaac ggacttgtca aacagtcacc atgcaactga gtctttatct caggacaaga
    52261 aacacgcagg aaaggttgga aaaatgaaat taaaacaatt acctctggaa taccaaaagg
    52321 caattccagt tctgaaaaaa ttagagaatg caggatatga agcttatttt gtaggtggaa
    52381 gtgttcgtga tatcttgctg aaccagccaa tccatgatgt ggatatcgca acaagcgcat
    52441 ttccagaaga aatcaaacag ctttttccaa aaacgataga tattgggatc gaacatggaa
    52501 ctgtgttggt attagaaaat gatgaacaat atgagatcac cacatttcgg acggaatcaa
    52561 cttatcagga ctaccgtaga ccagatcatg tcgaattcgt tcgttcgttg gcagaagatt
    52621 tgaaaagaag agattttacg atcaatgcgt ttgctttgaa agaagatgga gaaatcatcg
    52681 atttgttcga tggtctgcaa gatctagaag aacagatctt gcgagctgta ggcaatcctc
    52741 atgagcggtt ccatgaagat gccttacgaa tgatgcgcgg gttgcgtttt gtcagtcaac
    52801 tagggttcaa attagaggaa gaaaccttct acgccatcaa agaaaaccac gagctattag
    52861 gtaaaatatc tgtagaacgt ataaatgtgg aattcatcaa gatgctcttg ggaaatgaac
    52921 gggccgcggg aatccatgca tttattgaaa cagagtgcta catttgctgt cccggtttga
    52981 aagaagaagg cgaagcactt ttacgttttg ctgaattacc atccgtccca ttgaaagaag
    53041 agagccaagc atgggcatta ttgatcagcc aactggatct aaaagaaaat gagattcgtt
    53101 cctttttgaa atcgtggaag tgttctaacc aaatgattaa agacgttcaa gcacttgtcc
    53161 aaggactacg acaacgagaa actggagctt tggaaccatt cgaattgtac ttcttaggca
    53221 aaaaacatgc actgcaagta gaagaattga tgatctattt tgacgaaaca cctcagatgg
    53281 atgccgtagg agaatcctat gatcgtttac ctatcaagtc attaagcgaa ttagcagtag
    53341 atggtaaaat cttgctagaa gaaactggaa aaaaacctgg taagtgggtt agtgaagctt
    53401 tgcaattagc tgaaaaagcc gtcgtagaac aaagaataaa aaatgataaa aaacaggttt
    53461 tggatttttt atcgaaagaa aaattgattt aaaaaacact agactgtatc tgctcagctt
    53521 tgttggctac ttaaagtggg cagatacgtc attagaagaa aaaacataag atgtgaccac
    53581 ttacataaat ttgtgctaaa ctgaggtagt acaagaataa attacatcat aaaggagtct
    53641 ttttaatgag taaagaaatg acagcgctaa aattttattt tagaaatgga gaaacctgga
    53701 cgatcaaccg ccgtcatatc ggcgatcttt ggatcaaaca aatcacaaca agttttggac
    53761 gaatcaatgg cagtgaattt gttgaaatcc atccgtgtgc tggatttaaa atcgaaatct
    53821 tccatgaagg ggacgcagtt gcgacacatg atatcaacct aggcggactg gaaatgggca
    53881 tgtttaatcg cgcattgaaa tacgaagata tcgagcgaat ggaaatcctt taccgaaatg
    53941 gcacacctga tcttgtgtac ttcccatatc tagataaagg aacagaagga ttagacaatc
    54001 aatatcaatc aacaaaaatc agcgaaaaaa caggtaactt gtatatcgtg atcaatccag
    54061 atcaacgtgt agaagatgta tacggggaat ttttcgaata aatcgatttt tacgcaaaaa
    54121 tgaggctgtg aaagagatgt gtcttattac tactgatagg ctgtctcttc cacaccgttt
    54181 attcagaata agggactgtg acaagatttt tgtcgcagcc ccattttttt gttgtgataa
    54241 aagaacaaga aaggcaggaa tgggaaagta aacatccttt tgtcagaaac ttttatcttt
    54301 cttaagtttt acttgatatc tgatactatt tggaatagaa tgaggcgttg aaaaatttat
    54361 ggaaatttct aactagcaat aaacgatgaa acaatgaaat atttttcatc caaaaagaac
    54421 tttgctataa tgatggaagt ttgtgtatag gagatctgga aaatgagaga gagttttgaa
    54481 caacaaaaaa aactgttgca tgatcgctat ggcgcccttt cgatggatga ccgccgtcaa
    54541 atactttgta agttgcgtaa aagaaatata ttgatgtatc gtcagttgga acgtctgaaa
    54601 catgatctgc ttcgtttgga gtcaaaacgt gtacagtgtg aattagaggg gaaccaaaca
    54661 caggttgaag tagtcgaaac caaaatttta aagaaaaaag aacaattttt aaaaatgctt
    54721 acacaaaata aaaaataaag gggatggacg atggaatttg ctgagttagt tattgtattt
    54781 gcagtaacga tcaccttatc gaacatagct agccgcattt tgccaatgat tccagctcca
    54841 ctgatccaaa ttttccttgg agtggtatta ggtctgacag agtggggaca atcaatcgat
    54901 tttgaacctg aattgttctt agttatgatt attgcaccat tgttatttag agaaggagaa
    54961 aaagcagata tttcttcgat tctcaaaaat ttcgggacaa ttttgttctt ggcatttgga
    55021 ggagtcattt tgacccttgt cggggtaggt gcgacgttat cattcctgtt gccaagtgtc
    55081 ccacttgctg cctgctttgc ttttggagca gcgttaggtc cgacagatgc ggtagcagtc
    55141 agttctcttt ctggacgagt gaatatccct aaaaaggcga tgcatatttt ggaaggagaa
    55201 ggtcttttga atgatgcatc tggtgttact gccttccagt ttgctcttgg tgcgctgatc
    55261 accggatctt tttcagcagt aaacgcgggg atgagtctag tagtttctag tattggggga
    55321 gctttgatcg gctttttgct tgtctggttc aaacaaaaaa taattcattt gatcgaaaaa
    55381 gcatcagcac aagatgtcac agggtatctg ctgatcgaac ttttgcttcc ttttctagcc
    55441 tatgtattgg cagaggcatt tgatgtatct ggaatcattg ctgcagtggc agcaggtatt
    55501 ctccaagctt ctggttttag gaaaatctct gtgtttgatg cagaattgtc gagcttgtcc
    55561 cacagtacat ggacaactat tgcttttaca ctgaatgcat tagtatttat atttttagga
    55621 atcgaattga cgcaagtgtt ctcgccagta tggggagacg gtttatatcc aaacggtctt
    55681 ttattagcga ttatcgtatt aatctctgtc atgctttttg tgatcagatt catttctatc
    55741 agtctttttt atgtattcaa agatggcagt aaaaagttca aaaaacagtt gaacgagatc
    55801 ttgatcttaa catttggcgg tgtaaaggga acagtcagtt tggctacgat ttttattttg
    55861 ccattttcaa tcaataatat gatgttttac cagcggtctt tgcttttgtt tctgacggcg
    55921 ggtgtcatct tagtgacttt ggtcataggg atcattgtct tgcctatgct gacggaaaca
    55981 gaggaagcga aaagcacgga tttgaatgct ttgatgattt tagaagaggt cgttgaagtt
    56041 ttgcgaaaag aaataaagga aattgatcgt aatacaaaag aatttttagc cactgaagca
    56101 gtgatagaaa attatcaaga acggatacgt gatctgtact tggaagattt aaccgatgat
    56161 gaaaagcaag aagtacaaga aatacaagca ctgattttgt cgatcgaacg ggatggattg
    56221 gatgaaagtt atcgttcggg caagctttct tctaatggtt atcgttttta ctcccgtttt
    56281 ctttctcgat tcgaacattc tatcacaagc cagattttgt cttttatcgg cttttggttc
    56341 attgttgtcc gtaggttaat gcgaatcgtt ctccatccga aaatgttttg gcaaagaaga
    56401 tatgctgatc gtcagacatt tattagtgat gaagatatcc aagaaatcag agagacatat
    56461 caaaaaaata ctcagttgat tattgaaagt ttagataact tgactgatat ttatgatgaa
    56521 acgatggttc aattttttat ccagcaacgc aaacttgaag gaataaaaat gaactcgggg
    56581 aatttgattt cttcgtggat gatccagcaa gatagtctgt ttacgaaaaa aatgctaaga
    56641 ggttattatc ttgaaagaaa aatcattgat gaatacgaag ttgctgaaaa aatcacgacg
    56701 ttttctgcga acagctatcg caggaatatc aatttgttag aatcttatac gatgaacaaa
    56761 ccatctgata acttttcttt ccgatttgcg tttcgagcaa agtcaaaaaa aacgagtcca
    56821 ataaaataaa a
//
LOCUS       DAIGOK010000002        51644 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.002, whole genome shotgun sequence.
ACCESSION   DAIGOK010000002 DAIGOK010000000
VERSION     DAIGOK010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51644
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.002"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(153..731)
                     /gene="pgsA"
                     /locus_tag="NOA01_000049"
     CDS             complement(153..731)
                     /gene="pgsA"
                     /locus_tag="NOA01_000049"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCI0631290.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(940..1863)
                     /locus_tag="NOA01_000050"
     CDS             complement(940..1863)
                     /locus_tag="NOA01_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCI0631291.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(2602..3888)
                     /locus_tag="NOA01_000051"
     CDS             complement(2602..3888)
                     /locus_tag="NOA01_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCI0631292.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(3881..5149)
                     /locus_tag="NOA01_000052"
     CDS             complement(3881..5149)
                     /locus_tag="NOA01_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCI0631293.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            5401..5862
                     /gene="mscL"
                     /locus_tag="NOA01_000053"
     CDS             5401..5862
                     /gene="mscL"
                     /locus_tag="NOA01_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCI0631294.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(5921..6367)
                     /locus_tag="NOA01_000054"
     CDS             complement(5921..6367)
                     /locus_tag="NOA01_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCI0631295.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(6460..6705)
                     /locus_tag="NOA01_000055"
     CDS             complement(6460..6705)
                     /locus_tag="NOA01_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HCI0631296.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(6820..7398)
                     /locus_tag="NOA01_000056"
     CDS             complement(6820..7398)
                     /locus_tag="NOA01_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCI0631297.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(7475..8005)
                     /locus_tag="NOA01_000057"
     CDS             complement(7475..8005)
                     /locus_tag="NOA01_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HCI0631298.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(8009..8869)
                     /gene="rlmB"
                     /locus_tag="NOA01_000058"
     CDS             complement(8009..8869)
                     /gene="rlmB"
                     /locus_tag="NOA01_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCI0631299.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(8862..9257)
                     /locus_tag="NOA01_000059"
     CDS             complement(8862..9257)
                     /locus_tag="NOA01_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HCI0631300.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(9254..10666)
                     /locus_tag="NOA01_000060"
     CDS             complement(9254..10666)
                     /locus_tag="NOA01_000060"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCI0631301.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(10663..11199)
                     /gene="cysE"
                     /locus_tag="NOA01_000061"
     CDS             complement(10663..11199)
                     /gene="cysE"
                     /locus_tag="NOA01_000061"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCI0631302.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(11285..11524)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(11521..12981)
                     /locus_tag="NOA01_000062"
     CDS             complement(11521..12981)
                     /locus_tag="NOA01_000062"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCI0631303.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(13151..14308)
                     /locus_tag="NOA01_000063"
     CDS             complement(13151..14308)
                     /locus_tag="NOA01_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HCI0631304.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(14342..15712)
                     /gene="radA"
                     /locus_tag="NOA01_000064"
     CDS             complement(14342..15712)
                     /gene="radA"
                     /locus_tag="NOA01_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCI0631305.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(15775..16302)
                     /locus_tag="NOA01_000065"
     CDS             complement(15775..16302)
                     /locus_tag="NOA01_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCI0631306.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            16532..17338
                     /gene="proB"
                     /locus_tag="NOA01_000066"
     CDS             16532..17338
                     /gene="proB"
                     /locus_tag="NOA01_000066"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCI0631307.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            17335..18582
                     /locus_tag="NOA01_000067"
     CDS             17335..18582
                     /locus_tag="NOA01_000067"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCI0631308.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            18658..19002
                     /locus_tag="NOA01_000068"
     CDS             18658..19002
                     /locus_tag="NOA01_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631309.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(19119..19445)
                     /locus_tag="NOA01_000069"
     CDS             complement(19119..19445)
                     /locus_tag="NOA01_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631310.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(19818..21019)
                     /locus_tag="NOA01_000070"
                     /pseudo
     CDS             complement(19818..21019)
                     /locus_tag="NOA01_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            21136..21651
                     /locus_tag="NOA01_000071"
     CDS             21136..21651
                     /locus_tag="NOA01_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCI0631311.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(21850..22296)
                     /locus_tag="NOA01_000072"
     CDS             complement(21850..22296)
                     /locus_tag="NOA01_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HCI0631312.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(22343..23821)
                     /gene="msr(C)"
                     /locus_tag="NOA01_000073"
     CDS             complement(22343..23821)
                     /gene="msr(C)"
                     /locus_tag="NOA01_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HCI0631313.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(24175..24804)
                     /locus_tag="NOA01_000074"
     CDS             complement(24175..24804)
                     /locus_tag="NOA01_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCI0631314.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(24788..25252)
                     /locus_tag="NOA01_000075"
     CDS             complement(24788..25252)
                     /locus_tag="NOA01_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCI0631315.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(25739..27007)
                     /locus_tag="NOA01_000076"
     CDS             complement(25739..27007)
                     /locus_tag="NOA01_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCI0631316.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(27238..27975)
                     /locus_tag="NOA01_000077"
     CDS             complement(27238..27975)
                     /locus_tag="NOA01_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCI0631317.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(28042..28611)
                     /locus_tag="NOA01_000078"
     CDS             complement(28042..28611)
                     /locus_tag="NOA01_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCI0631318.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            28739..29611
                     /locus_tag="NOA01_000079"
     CDS             28739..29611
                     /locus_tag="NOA01_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCI0631319.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            29953..30825
                     /locus_tag="NOA01_000080"
     CDS             29953..30825
                     /locus_tag="NOA01_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCI0631320.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            30822..32780
                     /locus_tag="NOA01_000081"
     CDS             30822..32780
                     /locus_tag="NOA01_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCI0631321.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(33031..33882)
                     /locus_tag="NOA01_000082"
     CDS             complement(33031..33882)
                     /locus_tag="NOA01_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCI0631322.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            34005..34472
                     /locus_tag="NOA01_000083"
     CDS             34005..34472
                     /locus_tag="NOA01_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCI0631323.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(34735..35217)
                     /locus_tag="NOA01_000084"
     CDS             complement(34735..35217)
                     /locus_tag="NOA01_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HCI0631324.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(35317..36180)
                     /locus_tag="NOA01_000085"
     CDS             complement(35317..36180)
                     /locus_tag="NOA01_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCI0631325.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            36778..37626
                     /locus_tag="NOA01_000086"
     CDS             36778..37626
                     /locus_tag="NOA01_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631326.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(37797..38138)
                     /locus_tag="NOA01_000087"
     CDS             complement(37797..38138)
                     /locus_tag="NOA01_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HCI0631327.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            38224..38931
                     /locus_tag="NOA01_000088"
     CDS             38224..38931
                     /locus_tag="NOA01_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCI0631328.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            38918..39241
                     /locus_tag="NOA01_000089"
     CDS             38918..39241
                     /locus_tag="NOA01_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCI0631329.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(39330..40367)
                     /locus_tag="NOA01_000090"
     CDS             complement(39330..40367)
                     /locus_tag="NOA01_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HCI0631330.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(40814..41797)
                     /locus_tag="NOA01_000091"
     CDS             complement(40814..41797)
                     /locus_tag="NOA01_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HCI0631331.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(42213..42983)
                     /locus_tag="NOA01_000092"
     CDS             complement(42213..42983)
                     /locus_tag="NOA01_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI0631332.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(42976..43911)
                     /locus_tag="NOA01_000093"
     CDS             complement(42976..43911)
                     /locus_tag="NOA01_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0631333.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(44505..46154)
                     /locus_tag="NOA01_000094"
     CDS             complement(44505..46154)
                     /locus_tag="NOA01_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCI0631334.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(46349..47245)
                     /locus_tag="NOA01_000095"
     CDS             complement(46349..47245)
                     /locus_tag="NOA01_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCI0631335.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(47238..47918)
                     /locus_tag="NOA01_000096"
     CDS             complement(47238..47918)
                     /locus_tag="NOA01_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCI0631336.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(47919..48701)
                     /locus_tag="NOA01_000097"
     CDS             complement(47919..48701)
                     /locus_tag="NOA01_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI0631337.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(48694..49611)
                     /locus_tag="NOA01_000098"
     CDS             complement(48694..49611)
                     /locus_tag="NOA01_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCI0631338.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(49614..49898)
                     /locus_tag="NOA01_000099"
     CDS             complement(49614..49898)
                     /locus_tag="NOA01_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631339.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            complement(50599..51219)
                     /locus_tag="NOA01_000100"
     CDS             complement(50599..51219)
                     /locus_tag="NOA01_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631340.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            complement(51300..>51644)
                     /locus_tag="NOA01_000101"
     CDS             complement(51300..>51644)
                     /locus_tag="NOA01_000101"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631341.1"
                     /translation="SNIKTSTKLICKLFKLDKISNIDDVKSKILTRKVSEPVKLTAQD
                     KKNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSGEKVTFKINFITAISKLEYGKQK
                     KRDNNDQYLPKK"
BASE COUNT        14901 a        10939 c         9170 g        16634 t
ORIGIN      
        1 cacagctcct tgttccaaat aaacagtgtt ctcctgccaa aacctcggtc ataagtcaca
       61 atatacataa aacatgaaaa gctgtttttt gaaattatcc cttgtgactc agagttagac
      121 ggcaggctca caacctctcc actaaacgat acttacattg atcctttgaa cacaccgctg
      181 tttttgatga agtaatccac acctgaatag atagtaaaga tcagacaagc ataaagcatg
      241 atttgatcta ctggaatacc gatcaggctg aatgggatat tattgataaa cagcaagata
      301 atagcaacca tttgtgtagc cgtcttgact tttcccggcc aagctgcagc catgacttct
      361 ccatgttcga caagtagtaa acgcaagcct gtgacggcta attcacgaca gacaatgatc
      421 gctactaccc aaccaggggc ctttccttgg tcaaccaaca tgataaaagc agtcataact
      481 agcattttat ctgccaacgg gtccgcaaac ttgccaaaat tagtgacaag gcctcgcgca
      541 cgggcaattt ttccatctaa ccagtctgtg atactagcta aagcaaaaat aactgcacct
      601 actagctgtg tgacagccag tgtggtgtct cctacgttaa attgtcccca atctaaaggt
      661 aaacctacaa ccaaaatgaa aatcgggatc atacaaattc taagtacagt taatttattt
      721 ggtaaattca cactatcgac tcctttcttc cctattctac gtgactatca gcgaagtttc
      781 caccgatatt atttactttt ccaaatgaat ttattcaaag gagtattttt attgaaattg
      841 atctttttga attaacgaag aaggaccgcg acacagattt gtcacgatcc ttctttttta
      901 tacctacaag taaacagatg cgtaaactaa tttatacgat cattctgcgt attgaagagt
      961 aaggttcaca ttttttggac tctgattgtt ttcccctgga ttaactggta cggattctcc
     1021 attcgctttg atcgttgcat tgcttgctgc accaatcgtg atggtcgcat ttgttgctgt
     1081 ttccggtaac acggtactct gtgtttcatt tgctgccagt gtcccttgat aaacataggc
     1141 attgtctact agcacaccta accatacacg attgacagca gtaaattcaa gggttacagg
     1201 cttggtagcg tctgtgatat ttactgcgat ggcactgcca gaatcttctc ctgtagctat
     1261 tgccattttc ttttcttcac ttgattttgt tgttgattct actgtagaag actctttggt
     1321 ctgcgtgctt tgctctgagg aagaggactc ctcgactgat ccttctactt taagcgaaga
     1381 agctgtgtct ccgattattg gatcagcttg acgatcttgc caagtcatat agcccacgac
     1441 aacgacaatc gccaaagcaa ccaatcctaa taaaatgaca ggaagacttg tccaaaagcg
     1501 acttggatgc gattcttcct gatgcattgc ttttctagaa gtacttactg tttcaggctg
     1561 cggacgttca gggaccgatc ctgtcaattt gcttttccca tcaaaaacat cgactaaatg
     1621 gtctccatct tctcctacag cttcagcgta ttgccgaata aatgcccgca cgtaaaattt
     1681 accaggcaat tggtcaaaat catcattgtc gattgcttcc aaataacgtt tttgaatttt
     1741 tgtaatctgc tgtaattcat ctaaagatat ttttttattc aggcgtgctt gccgtaattt
     1801 ttctccgatg gtttcaaaag ccactcgttc atcttctcca gtctgtttga ttctccgaag
     1861 catctgctgt tgcaacgaaa gacgttatgt ttgggtgctg tgacaaaagt catgtcacag
     1921 ctcctttttt ccgaataaac attgttctcc tgccaactcc tcggtcacaa gtcacaatat
     1981 ccaataaata taagaagctg ttttttgaac ttgttccttg tgactcggag ttaaacggca
     2041 ggctcacgac ctctttctta aacggtgttc aatcagctgt tcctcggtat tagctcaaaa
     2101 accggcaaaa tataaggaga tatttttccg tatgtttctc taatactcag gtcaaaacgc
     2161 tgatttcaca acctcttcct taaacggtgt tcaaaagctt gcatctgcgg taataagtcc
     2221 aaccaagtta aaaacattaa gaagctgttt ttacttggct gttcttatta cttgatgcag
     2281 ggcagctttt tcacaacctc ttggtacacg gtgttccaga agtagcttct tcggcaataa
     2341 gccgaaatat cacaaaaatt tgagaagcaa ttttcgtaaa ttccttctta tttcttgaag
     2401 ctgaccactt ctgtcacaac ctctttgatt acttcgtttg attttatttt aatataagtt
     2461 gattaaaaga gagcatgatt tgttttattt tctcactaat ttttccaaca caactaagaa
     2521 taccataaaa gctgattttg gaggaggttc ctactacaag tctaaaacga aatttaagac
     2581 ttttcctcat aaaatactgg tttattttgg tctcatgtaa aactcgctga aggcctcttc
     2641 acttataaat gcttccccag ctgccagtac gtctttcatt tggatcgaat cgatgatttc
     2701 aggaagatca aataaagttc gatcgccaaa taaactttgc gtaaattgat tggcaatata
     2761 ttcgatcgaa tttaatgatt ggaaatattg tccaagcatt ttcttcttca gcaaatcgag
     2821 atttgtttct gacacttccg gatcatcagc gaatccaaga atgatttctt tgactttttc
     2881 tgctgctttc tctggttcgt ccgtatctcc gctgaagtcc gcaaagtgaa attcacgatc
     2941 caagctgaat tcaaaaccaa agctgtcatc gataattcct tgattgtaca tcgctagata
     3001 gtttcgagaa gtattgccta aaatcatttg gaacaacaga ttgattgctg ttttatagcg
     3061 taacagttct gttccttctt gcggcaatgt gtctaatcct ttaataccaa gtacaaattt
     3121 atctcgtgta atggctgctt ccaatgcact ttgtttgatg atttcttttg tgttttcagg
     3181 gaaataacgt acaatttctt gctttggagg gaaatttttt gcttcttggt tttcccgaat
     3241 aagtttcatc aatttctcag gctccatctt accaactaca aataaaacca tattgcttgg
     3301 ctgataaaac gtccgatagc acgtatacaa gtcttgagct gtgattttgt cgatactttc
     3361 aaccgtgccc gcaatatcga tatgcaatgg gtgtgtagga tacaagttat ttagtatgcc
     3421 aaaaaacatc cgccagttag ggtcgtcttc atacatttgg atttcctgac cgatgattcc
     3481 tttttccttg tttacagttt cttctgtgaa gtaaggtgct tgtacaaaat cgatcagagt
     3541 cgtcagattc ttttctacct gatccgtcgt agaaaacaga tagctggttt tcgtaaaact
     3601 tgtgaaagca ttggcggaag ctccttgttt cccaaataat tgaaagacat caccatcttc
     3661 tttttcaaac aacttatgtt ctaaaaaatg ggcaattcca tctggaactt tcaccttttc
     3721 tttttcacca tagggaataa attcgttatc gatcgaacca taattcgtac tgaacaaacc
     3781 atatgtcttg tgatagtcat ttttaggcaa caaatacacg gtcaaaccgt ttggcagtac
     3841 ttcatgataa agtgtttcat tgatttgctc atattctgtt ttattcattt gcatttcctc
     3901 ctgctaaaaa gaagattgcc tgcaattcga tttgctcagc tacttgttgt atctctttga
     3961 tcgttacgct ctccatacgt ttcaaccatt cttcatcaga taattttgtt tccggcagcc
     4021 agctgtccaa aaaagaagct tcgattgccg cttgcggaga gtctagagaa agcagatatt
     4081 ggtttctcag cattgctttt gtctgagaca gttccaactc tgttatttca ccatttcgca
     4141 aactttctaa ttgctcgtga atcagatgaa gtacttgatt tcggttcttt tcatcgatac
     4201 cggtttggac actcatgaat cctcggaacg tatccacgct actagaggca taataagcta
     4261 gactttcttt ctctctgaca ttcatgaaaa gtttagagtg aggaaaaccg ccaaataaac
     4321 cgttaaagac cattagcgca aaacgttctg gttcatcgta atacacattt gtctgataag
     4381 ctaggttcag ctttgcttga acgattggct cacgtacttg tcgttccttc acgatattca
     4441 cttgaggttg ggtataaaac agatctggat aaactgtttc ccgcgactca aatggtaatt
     4501 gcccaactgc ttcagctgct ttgtccggat caacatcgcc gacaacaaaa atgtcgattt
     4561 gatcatttgc catcatttct tgatagtagg acgctagcga actagcagtg atcttgtcca
     4621 aatcttcgac tacgcccaac cctgggatct tttgttccgg gctgttttca aaataaagtt
     4681 cttgcaaggc ataggaagca aaagtttgtt tatcttcctt caaactttcc aaatatagac
     4741 gcagattgtt cttttccaag tcaaacgtca actgatcaaa ctgatgattt ttaatatttg
     4801 gataaaataa aacctctttt aaaaaatcta ccgcttgaga aaaaaggttt ggatcattga
     4861 catattcacc attcacaaaa ctgatccctg cgtttagcca atgtaaattt cctttttttc
     4921 taactgataa tccaaagctt gcgccataaa gctctgcaag ttgcgcactt aactttgtct
     4981 gatccggata attcaaacta tttgtttcca acatgctgct gagaagtgaa cgtttcgtga
     5041 tgacttcttg ttgcagacgc gcagtaaaac ggataaaaat ccggatcgtt ttgtattgtt
     5101 ttgttggtaa gacatgaaga ttcacgcctt ctgccaattg atacaccata tttatatgct
     5161 ccttttcaca gttgattgcc cgcttactct tttaatgggt caacctgcct tttttactta
     5221 ttcctctgct tgaaatttat aagttcaggc aattcgccgt tttttctaaa tgatgttaca
     5281 ctttatagta tagcatagat gttttttatc aaaagaaaca gacggctaaa agcgtatatt
     5341 gtttttttat tttttatttt tcgatatact aaagacaaat aaaagaaggg agggtcattc
     5401 atgatcaaag aattcaaaga gttcatcatg cgtggagatg tcttggatct tgctgtcggc
     5461 gttgttattg gtagtgcgtt tacaagcatc gtaaaccaag tggtgcaagg cttgatcaca
     5521 ccactaatag gttggtttgt cgcaatgatc acaggtactt ctgatttaga aggagcactt
     5581 tcaattcttg actggtcgcc gacaaaaggc gtcacatttg cgtttggtaa cgttattagt
     5641 gctatcatta catttctgat cactggtttt gtcttattct tagttgtcaa agcggcaaac
     5701 cgtgcgaaga atttacgctc caaagaagag caagtggaag tggaagaaac tcctactgca
     5761 gaagattatc ttagtgacat acgcgatctt cttgccaaac aagtggcaga agacaataca
     5821 catccagtag agcaaacaaa tctttccgat acttctaaat aataaagaat ctaaaaaaaa
     5881 gaaagccgat tttcagtgtc ggctttcttt ttttagattc ttaacttgct acttgttcct
     5941 ttttcatgtc gatatgtgga atatcatctt ccaaatacac atctgagatt gccttgaatc
     6001 caaacgactc ataaaaagct tgtaggtatt cttgtgcgcc gatcacgatt ggtttttctg
     6061 gaaaattttt ctggatcatg ttaatcgttt cttgaacaat ctttcttcct aaaccatctt
     6121 tacggttgtc ctgatgaaca aggacacgtc caaaatggat actctccgct tcttcatata
     6181 ctcgcgtgta ggcaaggatc ttcccatcag tatcttgaaa aaacgtatgc caagcttgtc
     6241 catcaatgtc atcaacttct tgataaggac actgttgttc cacaacaaaa actgcgattc
     6301 gttctttgac gatcgcgtaa aactctgctg ttgacaattc ttcaaatttc tttacctgat
     6361 attccactaa gcaaacgccc cctgcttttt tattcttagc ataccgattc gcacacaaaa
     6421 aagcaaacga accttacgaa tctcaggctc gtttgctttt taaactgctt cagttaaaaa
     6481 ttcgatctca actgtccgag taagtacatc tgaatagctg taagatactc gttcaaaaga
     6541 attttcttca ggatccaaat caacaacgaa tactgaagga taggtttctg ttaaaacgcc
     6601 gcggcgttct gtttgacgct ttcttcctgt ctgagcaacc aacatgattg ggctgccgat
     6661 acgaccttct aaatctttct tgattgaagc taaagttgtt ggcatacagt tcacctctac
     6721 tatcaactat accacatatt tttaaaattt tcaagtttac cataaaaaaa aaagaaatgc
     6781 aataaaaata aataagaagc tcattaagat ttttctaaat tattcctcat tataaaattt
     6841 caagtgtttt tttattttga gttttactct cccgtaagcg ctcattactt gcttgtgatc
     6901 cattgctaaa cattcagcca tgtctacaaa atcaacgcct tgcatataat aatgaaaaat
     6961 tttagcttct atttttgaaa actggatttc gcaattttcg attgcttctt ttaataaaag
     7021 atgctgaatc gctaaaggtt cttgcaatgt gatctctgct aaaaagtctg gtccttctgt
     7081 ctctattttt tggtcgattg agacttcatc tttttgatca cgacgcttca gcgcgttctg
     7141 cttcctcaac agagaataga tttgattgct tataatgctt ttataataac ttccaaagct
     7201 aacacctttg cttttttcgt aagttctcaa tgcctgatat aaagcgatcc ttgcttcttg
     7261 ttcccaatcc tcagaaacat aatttcttaa ataataggtg tttttcaaac gtagtaaaag
     7321 tggaaaatat ttttgataca gggcagcaaa tgcttcttcg tcaccattac gtgcagcgcg
     7381 caataactcc tcagacataa aataattcct ccataaattc gtttatggag gaatcttatc
     7441 atcaatgatt catcgaactg attagaacga tatgttattt attttttctc attaaatcgt
     7501 ccaatttttg ggacaatttt tttaactgct cttcattcca tggagaatta cgtcggaaat
     7561 caataaaatg catatcttta ttttcttggg ctattgtttt ttccgttttt tttatcgtct
     7621 tatataattc ataagcagaa gttcgtaatg ctccttggga aaagatcatc cactgttctg
     7681 ctaaatcgct tgtcgccact gtcacttgtg tcaaacggtc attcagctga ccagcgattc
     7741 tctcgatata actgtcagcc gtttcgtctt ctttcgtaaa aacaacatcc aactggtatt
     7801 tcttataatt ttgttggatg cccggaacta gttgggcatc gaagacaacg atgacttcca
     7861 ttccttcata tttcgcataa tttgataaac gatgtaaaag agcttctctt gcatccgcca
     7921 ttttgtcctg ctttttaagc tggacaagtt ccggccacga accaatcatg ttgtagccgt
     7981 ctacaatcaa cagctgtttt ttcatggtct actccccctg acgttgggaa aaagcctgat
     8041 acataagcaa accagcagca acactggcat ttaagctctg gacgtgcccg gtcatcggga
     8101 tcgtcagcaa ttcatccatt tcttttttca atcctggact cattcctttg ccttcattac
     8161 caatgactaa agcaattgat cctttggcat tccatttttg gaagtttgtg ccggtcatat
     8221 ctgtgccaaa tacccaaaat cctgtttctt tgagctgttg cacagcttgt accaaattcg
     8281 tcacgcgtgc aacagggaca tgttcgactg ccccagtgga agtctttgtt acgataggtg
     8341 tgattccaac tgctcggtgt tttggtatga tcacaccatc gacgccactt gcgtctgctg
     8401 tccgtaaaat cgacccaaaa ttgtgtggat cttcgatgct atccaaaatc aaaaagaagg
     8461 ggtcttcttt tttctctttt gttcgttcaa tcagttggtc aagggataaa tattcataag
     8521 gtgtaatcgc aagaacgact ccttgatgca cgccgtgatc gctcaaagtt tctaattttt
     8581 gtttaggaac ccatttgact ggcacagctt gttcacgtgc gatttctttc agttcttcta
     8641 ctttttctcc tcgtgaatct tcttgaagaa agagtttatt ccctcgccct tgttgtagcg
     8701 cttctactgc ggcatggtgc ccaaagacga aattatcttc taatgcttct tcattttgat
     8761 cagaaactga ctgatttcgt ccagtttgct ttttatttga atatctattt tgttttttgt
     8821 cttcaaatcc acgtttcgat tgatgtttgt ttgttctttc tttacccatt tttttcacca
     8881 actttctgaa tacaccagcg gatcaattct tccatccgct cggtttgttt tgtcaaatgc
     8941 agatagccca tgagcgcttc gaaccctgtt gcgacccgat acgtcgtaac atcagcattt
     9001 tttgcgctcg tatggctttt agcatttctt cctctgcgat aataaagttc ttcttcttct
     9061 gtcaaaagat gttcttccag catttgctga atcaaaaagg cttgtgcatt cgcagaaaca
     9121 taattcgttg ccgcacgatg cagcttattg ggttttgttt ctccttgatc aatcaggtgg
     9181 tcacgaacat agatttcgta aatcgcatct ccaacataag ccaaggctaa accgttcaat
     9241 tgtgtgtaat ctctcatgct tctcttctcc atcgggttcc ttgcggtgta tcttctaata
     9301 agatcccttt ttctttcaac aaatcacgaa tttcgtcgct tcggacaaaa tcacgattgc
     9361 ttcttgcttg atttcgttct tcgatcaatt gttcaatctc ttcatctaat aactcttctg
     9421 ctgtaaataa aataccgaag atcgaaagcc attgcgagaa taaagcttct ccttgtgcca
     9481 agacagcggc agaaactttc ggctggtcta gataagtgtt cagccactta gcaagctcat
     9541 agaccactgt gatcccattt gctgcattaa aatcatcgtc catttcttcg atgaatcgct
     9601 gttctaaatt ttctaacaaa gcgattttat ccgcatcatc tgccaaattc tctgaggcag
     9661 tttcttggcg gaatttcaaa ttgtcaaacg tattcttcag tcgttgtaaa ttggtggctg
     9721 cttctttcat cgtcgtttca ctgtagcgga tcggacgacg gtattgtgtg gtcgccatga
     9781 agaaacgcaa gacttgtgga tcaacgtttt tgatcatttc atgtacagtg atgaaattcc
     9841 ctagagattt actcattttt tcatcgtctt cgccaatcgt tacatagcca ttgtgcatcc
     9901 agtaattagc gaattttttc cctgttttcg cttcactttg tgcgatttca ttttcatgat
     9961 gaggaaattc taaatcttgc ccgccaccat ggatatcgat cgtttctcct aaatgttttg
    10021 ttgccataac ggaacattca atatgccaac cgggacgtcc tttgccccat ggtgaatccc
    10081 aagaaatctc gccaggtttt gcttgtttcc ataaagcgaa atctaatggg tcttcttttt
    10141 gctgctgttc ttcccctgta cgctgacttg ctccgacttc cagttcatcg atggattggt
    10201 cacttaattt cccataattt tcaaatttac gggtacggta atacacatca cctgaagatt
    10261 cataagcata gcctttttca accaacactt gaataaatgc taaaatatct ggcatatgat
    10321 ccatgactct tggatgcagt gtagcaggct cgacattcaa cgctttcgta tcttcttcaa
    10381 atgctcggat gaaacgatca gcaacttctg gtgctgtaat acctaattcg tttgctgtac
    10441 ggatgatttt atcgtctaca tccgtgaaat tcgatacgaa atcgacttta tagcctcgat
    10501 attcaaaata gcgtcgaatt gtatcgaaag caatggtgct acgagcattt ccgatatgga
    10561 tataattata cacagtcggt ccgcagacat acataccgac atgtcccttt tgtataggga
    10621 tgaattcttc tttttttctg gtcaatgtat tgtagatttt tatcattctg atctcctcac
    10681 tttttctcct ttgattcgaa cgacttttgc tggtactcct accgctgtag cgtcgtctgg
    10741 aacatctttc aacacaacgg cacctgcacc gattttggcg ttttttccga tcgttaccgg
    10801 tcctaatatc tgcgcattcg cagagagcat ggctccttgt ttaacagttg ggtgtctttt
    10861 acccgtttct tttcctgtgc cgcctaatgt tacgccatgg aataaaatca catcgtcctc
    10921 gatttctgcg gtttcgccaa tgacgattcc cattccatgg tcgatgaaca cgccagttcc
    10981 aatcgttgct cctggatgga tctcaacacc agttaggaaa cgccagaatt gagcattgat
    11041 cttcgccaat aaaaagagcc ggtgctgata taaaaaatga gagaaacgat gccaaaacaa
    11101 ggcatgaaaa ccagggtatg tcaaaacgat ctcagctgtc gaacgtgcag ccggatcgtt
    11161 tctctttgct gcatcaatcg ctcgggtaaa ccaactcatg actttccact cctttcgtaa
    11221 gaattttatg taggaaatca tcttcttata aagaaaagaa gcgcctttta aataagtttc
    11281 cttatcaaaa gacgctttcc gcgtggttcc acttttcttc ataatcaatg attatctctg
    11341 acagctaacg cctgttatac gttcttggct actttgtttc ctataaaaca gtttttcctg
    11401 ttttctgtgt ttcgccaaga ccactcccag aggcatttcg gcaaccattc attgatacct
    11461 tgcaccaccc ggtatctctc ttgtaatgat cagctgctta cttttttctg ttcatcgtgt
    11521 ttagttcagt acttgattta aatgtgctaa tgatttttca cgaccaagca attcgatcgt
    11581 ttcaggcaat tctggaccat gcatttgtcc tgatacagct acacggattg gcatgaaaag
    11641 attttttcct tttacgccag tttctttttg aacagctttg atggcagctt tgacagttgg
    11701 agcatctact acttccattt gttctagctg tgctttgaat gcgtggagaa cagtaggaac
    11761 tgtttcacca gcaagtactt cttttgctgc ttcattcaat acaggatgtt cattgaaaaa
    11821 tagcgtagac agatcaacga tttctgcagc atagctcatt tgaggttgat acaggctaac
    11881 gatcttcttc acccattcga ttgtcttttc atctgggttt tctgctacac gtccatcagc
    11941 gattagataa ggcagacaca tgtcagtaaa tgcatctaga tccattgctt tgatgtaatg
    12001 gttgtttacc cattgcagtt ttttcgcatc gaaagctgcg ggtgatttac ttaggcgttt
    12061 tggatcgaac attttgatca attcttggtg ttcgaagatt tcgtcttcgc ccacaggtga
    12121 ccagcccaat aatgcaataa agttgaacat tgcatctggt agatagccta attcgcgata
    12181 ttgttcgata aattgcaaga ttgattcgtc acgtttgctt agttttttac ctgtttcact
    12241 gttgatgatc aatgtcatat gtccgaaggt tggtggtgtc cagccaaatg cttcatagat
    12301 catcagttgt tttggtgtat tggcaatatg gtcatctcca cgtaaaacgt gagtgatttt
    12361 catcaaatga tcatcgaccg caacagcaaa attataagtt ggcataccat cgcgtttttg
    12421 tatgacaaag tcgccaccga tattatcgga ttcgaacgaa atagcgcctt tcaccatatc
    12481 tgtaaatgcg tattctgtat tgcgaggcac acggaaacgt acaacagatt ctagaccttg
    12541 tgcttctttt tctgcttgtt cttcaggtgt caagttcgca catgttcctg cgtaatgagg
    12601 catttctcct cttgcgcgtt gtgcttcccg ttctgcttct aattcttctt ctgtgcaata
    12661 gcatttgtat gctcgattgc tagcaagcag ctgatcaatc agcggttgat agatttcttt
    12721 acgttcagat tgacggtaag ggccgtattc tccaggattt tcaggagatt catcccaatt
    12781 cattcccaac caagcaaggt tttccaactg gcttttttcg ccatcttcga tattacgttt
    12841 ttggtcagta tcctcaatac gaataataaa gtctccatca ttgtggcggg caaacaaata
    12901 attgaataat gcagttcttg catttccaat atgcaggtgc ccagttggac ttggtgcata
    12961 gcgtacgcgt actttcgtca ttttcttcct cttctttcaa actagacttt gactaacagc
    13021 aaaaattccg tcagttacgg cttctcaaat atcatttatt taaagccact cagcacaaat
    13081 tttacctttt ctaaggtaaa tagtaaagcc aactggttga atttccttac cagttggctg
    13141 aatttctttt ttattttgat tgtttagtct ttttctctgt ctctttttta ttgtcgattc
    13201 ctcgtcctgc atgtgcaggt ttagcaaaga tcatgcgtcc agcagctgtt tgaagggcac
    13261 ttgttacaac gacttcgata cgttcattca tgtaatgctg tccatcttct acgacaacca
    13321 tcgtgccgtc atcaaggtag gctactcctt gttggcgttc tgtcccggct tttactacta
    13381 gaacattcat cgtttcacca gggatgacga ctggttttac ggcatttgcc aacgcattga
    13441 tattaagaac aggtacattt tggaattcag agactttatt caagttatag tcattggtta
    13501 caatgacacc atctaaaagc ttagccaatt tgatcaattt gctgtctact tcactgatat
    13561 cttcaaagtc tccatcgtac atttcgacag atataccctc ttctttttgc aaggcattta
    13621 ggatatctaa tccgcgacga ccacgcacac gtttcaaact atcccctgag tccgcgatgt
    13681 attgtagttc gtaaagaaca aaattaggga tcatcaaggt tccttctaag aatccagtct
    13741 ttgtaatatc ataaatccga ccgtcaatga tcacgcttgt atccaagatt ttatatttat
    13801 ggaaattttc tcctgatttt cgttctaaca gttgactctc aacttgacta gctgtttctt
    13861 gctcgatttt tttgcttcgt gaaccaaaaa ctttcttcca ttcttcgatt cgtgtcgttc
    13921 ccattctcaa tcccagatag cctaagatga tcatcagtaa aatcggcaat acactattga
    13981 cgaatggaat ttccatattg taaagagggg tcgagatcac aacacctaaa gtcaatccga
    14041 tgatcgcacc gatcgcaccg aagagaagat aagtcaagct gacctcactt agtttttgtt
    14101 ctacttgttt gataatagct gagatatgtt tggccaacag caaagataat aaaaagaaaa
    14161 taagtgcgcc taacaggctg ttggtaaatg tattattcag ccatccgttt gactgttgac
    14221 cgattgcctg ccaagctgag ggtaaaaatg aaatacccaa gcttgcgccg acaatgatca
    14281 tcagcaaagt aatgacacgt ttttgcatcg tttttcctcc gtttcgacaa tcatttatta
    14341 gttagcggaa cacacgcttc aacgtttcag ccaaagttgc tactccaact atctcgattc
    14401 cttttggcgc ttcccagtta cctaagttgt ttttaggtag gtatactttt gtgaagccga
    14461 gtttttggac ttctttgact cgttgttcaa tatgactgac acgtctgatt tctccagtca
    14521 gtccaatttc accgatgaaa cattccgttg gctgtgttcc tttttcttta tagcttgaag
    14581 caatactgac agcgatagct aaatcaatcg ctggttcatt taattttacc ccacctgcag
    14641 cttttaagta agcatcttga ttctgaagaa gcaacccagc tcttttttcc agcactgcca
    14701 tgatcagaga tacccgattg aaatcaagac ctgttgttgt tcgtttggca tttccgaaca
    14761 tagtaggtgt gactaatgct tggatctcta ccaaaattgg acgtgtccct tccattgcca
    14821 caacgatagc agagccagtt gcaccgtcta ggcgttcttc taagaaaatc tgtgaaggat
    14881 tagcgacttc ttccaatcca tgctcatgca tttcaaaaat cccaatctca ttcgttgagc
    14941 caaatcggtt ttttactgct cgcaaaatcc gaaaactatg atgttgttca ccttcaaaat
    15001 ataacaccgt atccaccata tgttctaaca ttcttgggcc ggcgatcgag ccttctttgg
    15061 taacatgacc gacgataaaa atggctatcc catttgtttt agcgattttg agtagttctg
    15121 cagtggtttc tcttacttgg ctgacacttc ctgccacact tgtgatgtct ggctgcgtca
    15181 tcgtttggat ggaatcaatg ataacataat caggggagat atgttcgatg gcacgactga
    15241 tttcgttcat atctgtttca gcgtatagat aaaattctgt gttgatactt ccgaggcgtt
    15301 ccgcgcgtaa ttttatctgc tctgcgcttt cttcgccgga tacatataag acttttccgc
    15361 ctatggctgc taattgctga gaaacttgta acagcaaagt ggatttcccg atacctggat
    15421 cgccaccaat caagaccata gaccctggca ccacacctcc gcctaacaca cggttcagtt
    15481 cttctaattt tgttttgatc cgaggttctt ttttagggac gacgtctgcg atttttgttg
    15541 gttttgcttt ttctcctgtc aaacttgtcc gagttctgcg gtccgttgta tcttgttcga
    15601 tttcttcgac catcgtattc cattttccgc aatttgggca gcgtcccaag aacttaggcg
    15661 acacataccc gcaattttgg caaacaaatt gtacttttga tttttttgcc attgtaccgc
    15721 ctccttttat cctaatcatg agacattaaa tcaagcttaa gtatctttaa cgttttattt
    15781 actggaagaa ccaaatccgc cttgacgttg agcagtcgtt tgatcttgat ctgcttttaa
    15841 aaacggcaaa aagatccctt ggccgatacg ctcgcctttt ttgatcacta catccttgaa
    15901 accaaagttg gtgaattgga acatgatatg gccttcattt gcctcattat tgtaataatc
    15961 actgtcaatc acacctactc cgtttgtcaa cactaaaaac cgcttcaacg gattgcttga
    16021 acggttagca agctgcaaaa attcatcttc tcccatgtaa gctttgattc cagtaggcac
    16081 gagggttggt ttcaattgtt tttcattttc tgatacatct tttttcaatc catctgcgac
    16141 tgtttccaac ccctttttcc agcctgcttt ccagatactt ggaatcacga cctcttctgc
    16201 tgcttcaaaa tcatagccag cagcatgatg tgtagcgcgc tgcggtagat tgatattttg
    16261 atctttatat ttagaaataa cttcaaatcc tctttttctc accggttttc ctccctaaat
    16321 aaactactcc tattctaaca tatccgaggc tgtaaaagac tttctttaca aattcttcaa
    16381 gaaattctgc tatcttaaag taaaccatta attcgttctt tttccaaacg actgcaagtt
    16441 gtacaaaaaa actatttttt acatgacttt actcgtcaaa acaagtagaa tagaacgaaa
    16501 ttaatgagtg gatatttgat tgaggtgtca catgcgtaaa gaaattaaaa atgcaaaacg
    16561 aattattgtc aaagtaggaa caagtacatt gatttatccc aatggaaata tcaatcttgc
    16621 ggcaatcgat cagttagctt ttgtcctttc tgatcttaga aatcggggaa aggaagtgat
    16681 cttagtctca tcaggagctg taggcgttgg tatgcataaa ttacatttgt cagaacgtcc
    16741 gactttgatt cctgaacagc aagctgcggc ggctgttggt caagccgaac tgatgaatat
    16801 ctataatcaa agattcagtg tatataatca acagacggca cagatcctcg tgacaagaga
    16861 cgtggtagat tatcctgaaa gtcgtaaaaa tgtgaggaat acattggaac agcttttgat
    16921 tatggacacg atccctatcg tgaacgaaaa cgatactgta gcagtggatg aattagatca
    16981 tctgacaaaa tttggcgata atgatcagct gtcagccatc atggcaaaac ttgtcgcggc
    17041 agaccttttg atcgtccttt ctgatatcga cgggttttat tcagataatc cgactacaaa
    17101 tcccgaggct cagatgtatc gctatatcga taaaatcgat gaaaagattc tggcacaagc
    17161 aggtggtgct ggtagcacat ttggaacagg cggtatgcag agtaaattaa aagccgcttc
    17221 taggatattt gaagcaaaca gcgcaatgat tttagcaaat ggaaaaaatc cggcaattat
    17281 ttttgatatt cttgcaggaa aagaagtagg tactttattt aaggaggact tgaaatgact
    17341 gacttgatgc aattaggaca acaggcaaaa gatagcgcgc aacaactagc acttatgcct
    17401 acaaaccgca aaaatgagct acttgttcaa atggcacaaa ccatcaaaaa gaatcaacaa
    17461 gcgatcattg aagcaaatca taaagactta gaaaaagcag tcgaaaacaa tatcagtgaa
    17521 acgatgcaag accgcttgca gctgacacct gagcgaatca atgccatggc tacagagatc
    17581 gaaaaaattg cttcgttaga cgatcctatt ggaaaagtag atgagatgtg gacgaacacc
    17641 gacggattga gaatcggcaa aaagcgcgtg ccactaggcg tgaccggcat catttacgaa
    17701 tctcgtccaa atgtcacgac agatgcagct agtctagcat tcaaatcagg gaatgccgtt
    17761 atccttcgcg gtggcaaaga agcattcttc tcgaatcaat tattagtcca attattgcag
    17821 caagtactgg tcagtgctgg cgaatctcct tatgccatcc agtttgtcga tgatacttct
    17881 catgaaactg caggacagtt gatgcgattg acagaatact tagatgtgct gattcctcga
    17941 ggcggagcaa aattaatcca acgagtcaaa gaacaggcaa cggtacctat cattgaaaca
    18001 ggtacaggaa ataatcacgt atatatcgat aaagaagcac agttatctat ggcagtgaac
    18061 atcatcgtca atgcaaaagc ttcccgtcca tctgtctgta acgcggcaga aacattgctg
    18121 atccattctg agatcgcacc ttttttcttg ccagctattg aaaaagaact ggtagaacac
    18181 ggggtctctt tacgtgcgga tgctcgtgcg ttagaatatc tagagacggc tgctttagca
    18241 gaagacacag attgggatac tgaatatcta gattatatcc ttgctgtcaa agtggtcgat
    18301 tctttggatg aagcaattgc acatatcaat cggcataata ctaaacattc agaaacgatc
    18361 gtgacagata gttatgcggc tagtcaacgt ttcttaaatg aagtggacgc ggcggctgtc
    18421 tatgtcaatg cctccactcg ttttacagac ggatcggtat ttggttttgg tgcagagatt
    18481 ggtatctcta ctcaaaagtt gcatgcccgt ggtccaatgg ggttaaatga attaacttcc
    18541 acaaaatata tcatttatgg cgatggacaa atccgcgaat aatgtttcgt gtgaaacatt
    18601 attcccgcct tttgcctttt ttctgtaaag gttctatcca actagaaagg tgtcaatatg
    18661 tttgaaattt taatgatttt ttttatttac cttatttctc tgaatatcgc tgcttttctg
    18721 ggagtcggta ttttatccct tttctttcaa ttcaaaaaac gaagcatagg gagtcaacgg
    18781 gaaaaatggt cgcagtattt tgacaaaatc gggcaaaaag ggctggttac acgcttgcac
    18841 attagttaca tggttgcatt atgcttatta gcgacaatca attactattc ttttttcgat
    18901 cattcaatcg cttatacgat tacgctactg attgctggga tttttcactt atcttataaa
    18961 tatcagctga ataaaaacca cttgaatcgg actttccgct aaaaaatatc cgtacgataa
    19021 gtagagtgcc ttgaagatcg gcaaaaaact gccggacttt ttagaggctg tgacaaagat
    19081 tttgtcacag cctctttttc catataaacg gcgttcgcct accagctgcc tggtaaatct
    19141 gtttcctctt cgttaagaag cgaaaatccg tcaaatgttt cgccatcata ataacgaagc
    19201 agctcatcca tggacaagtt catcgttttt tctttttctt cttccgctga ttcagccagt
    19261 agctgttccc gatacacctg atacatacca gctatttttt gctgcatcgc ttttctagtt
    19321 tcaccttccg cattgattat tggcaattct ttgatatagc cacgataaat caaccgatca
    19381 tctgaaaatt caatgggtaa ttcaacgagt gtcaacccga cttcctgaaa atattcttcc
    19441 agcatctatt atccccccat tctttttctt aaatgaattt tagcttacct actgaaaatt
    19501 ttccagtttc attattttga gaaaaaaagg gaatgtgaca aaaatcgtgt cacatcccct
    19561 ttttccgaat aaatggtgtt cttctgccaa ctcctcaatc ataagtcaca atattcaaca
    19621 aacatgagaa actgtttttt gaaattgttc cttgtgattc agagttaaac gacagtctca
    19681 caacctcttg gtaaacggcg ttcaaaagct tgcatctgcg gtaataagcc caaccaaacg
    19741 aaaaatatga gaagctattt ttgtttggct gttcttatga cttgatgcag gacagctttt
    19801 tcacaacctc tgatttctta ttcttcttca tttgtagcaa aataattacg gtgataaatg
    19861 atacaacata cactgataag aacaatggca cctgcaaaat aaaacgcata gctttcacca
    19921 atatgttcac caatcacacc tgcaataaat ggtgctaaag ctccaccgaa aaagcggaca
    19981 aaactatagc tggacgaagc aacgtttcgt tgcagacctg gtatttccat agcaatcgtc
    20041 gtcaacagtg tattgataat tccttggaaa aatcctgcta acacaatgct gatcggtaca
    20101 aggactggca tagaagttcc agctcctaat aacagcaaac aaataatgaa taagacaaat
    20161 gctgctaaaa ttgtccgata agtcgtaaac ttccgctcaa gagccggtgc aacgaaaatc
    20221 gaagcaatag ccagaagcac accccagcca aaaaatacaa aaccgacttc taattcagaa
    20281 tagccaatca ataaaaatgg cgcatacgca agtaaagtaa agaatccgaa attatataac
    20341 aatgcgatta gtccaactga acgaagttta tgattgctta acgctttgac accttcccaa
    20401 aggcctactt ttctctttgg tttctcagtt tcaggtaaaa gtaccgctag ggctataaaa
    20461 ccgcaaaaca tcaaaaatgc taccccaaaa aatggataac gccatgaacc actgcccaag
    20521 accccaccga ctagagggcc aacagccatt ccgcagccaa gtgctgcttc atacatcatg
    20581 attgcttttt ctgtgttacc aactaaaata ctgacgatcg ttgctaaagc agtagagaca
    20641 aataaagcat tcccgacacc ccagccggca cgtaacccaa tcagcattcc gatattccca
    20701 gataatccgc caagaccagc gaatacaacg atgataaaca atcctgtcat caatgttttt
    20761 tcaaaccgat ccgactagat aagaaacctg taaatagcat caccacacca gtgactaaca
    20821 tgtaactagt aaataacagg gtcgtttcag ctggcgtagc gtttaactgt gcagcaatcg
    20881 tttttaagat tgggtctact agacccactc ccataaaagc gatcatacag gtagctgcta
    20941 caactagcgc tgaacttttc ccctttgttt cttcatttgt ttgttcttgt atatctattt
    21001 gattctccaa tatttccatc cctttctctc tattagcatc atacatgatt tattttacat
    21061 gtaaatatgc acatgtaaaa cgtggaaact ttactgtgat ataataaaga aaataagacg
    21121 agaggtagga tttttatgaa tactttagcc tatatattat tatgcgtgct tttatccaaa
    21181 ccacgttctg ggtatgaatt gaagcagctg atcacaattt tttgggaagc ccatcacagt
    21241 cagatctata caacgcttgc caagttagac cagcagggtt atgtgaccgt tttggacaaa
    21301 gacgagcact ctcaaaaaaa gatctaccac ttgactgatg aaggacgtgc tcttgtagag
    21361 gaatggatca aagaggaaac tcccgctcct acgcaaaaag atgaatttct agcaaaaatc
    21421 tatgcttttt cgacgctcga taaaacaaca gctactggac tgctattcac tagagaacaa
    21481 cagctgaaca aaatcatgca aaaaaatcaa aaaaaattga atgacctctc gctagacaag
    21541 aaacaagaat ttggacgtta tgttgtgatc cagcgcagaa ttatgctttg tcagcaggaa
    21601 ctccaatggt gtcagcaagt aagagaacaa atgcagcagt tttttgagta agggataaac
    21661 agaacctttc tagaaatatc tattgaagag caaattatct tcaataaaag agcaatacag
    21721 ttggaaaaga aaaagcgatc cgaacgacag gccaaaacct atcgttcgga tcactatttt
    21781 tctagaactg cattcaagag actatgacaa aaagcatgcc aaggcctttt ttcaaataac
    21841 agtgttccat cattcattac tttgaattgc ttttagcaca cttcttgaaa gcgggccaac
    21901 gatgatcaac tgcaacggta aagcggcaat cacattcatt ttccaagcat tcatataagc
    21961 agagaataat tgatcaggta ttccttgttc catgatcaca ccgaataaag acatacatgt
    22021 caccattcct aaaaccatca agcatgaaat agttaagatc atttggacct tgctttctgg
    22081 atttactggt agtttaaagg caattttttt cgccagcaca ccgatgatga agacatccaa
    22141 aatgaaagcc accacgaatc caggcactag accaatcaac aaacttgata aatgaagatc
    22201 ttgatgcagc cataaattat acgcgctcat ccccaagacc atcaaaaaac acatcattgt
    22261 tgtaaaaatt attccttctt ttttatttgt aggcaatctg taccactcct ttaaatttgt
    22321 cacgaaaatc atcgtagcac atttaaaaat ttctcgtaag tacttttttt attttctgcc
    22381 cttttatttc ataaacttca tcagccgtac gctcgacaaa gtaagaatca tgagaagtaa
    22441 acaaaatcgt tcccggatat atttgcatta gcttctctaa agcttcgatc gttgccatat
    22501 caataaaatt agtcggttca tctaacagca acacattact tggcttagta aaaagcaaag
    22561 caatcgtcag acgggtcgct tctcccccac ttaatgtaca taacggtttc gtcaaagttt
    22621 cctgagcaaa ccctaagtga ttcagcaaac tacggacaag cgattctgaa aactctgttt
    22681 gtctcatgac aaaacgaatg atggattctt cagatgtcat ttgataaccc ttttgctgat
    22741 atacttgaaa gcttactttc ggagagagaa ggattccttc tccttgtttg cggatatgtt
    22801 ctaataaggt tgtcttacct gaaccatttt caccgacaat cgcgattttt ttacctaaag
    22861 aaaactgaaa atcaccattt accagtaatg ttctcccact tctttccaat tggacatttt
    22921 gtcccatgat gggatagcgg ctgtggagtt caagagattt tggtactgga aaacggattt
    22981 gtttcatctg ctgcggcttg gttgtttctc ctattttttg gagtcttctc tctaatgttt
    23041 tcgcattctt ttggatggct ttttgtacac tgtctttttg tttagaggaa gacaaccgat
    23101 ccggtctgat ttgttgcttt ttctttttac tggacacttt ttccaacttt tccgcttgtt
    23161 ttttcttttc ttggatcgat ttttccaatc gttttttctc tttcatgatc gactctgctt
    23221 ctcgtagctg agtctttttc tccaattcct tttgagtgag ataggcacta taattccccg
    23281 aaaactctcg gatttctccg tcttttactt cccagatttt cgatgctagc tcatctagaa
    23341 aatagcgatc atgactaaca aaaaccagcg taccataata ataacgaagc tcttcaatca
    23401 gtcgatcaat actttttcta tctagatggg ttgttggctc gtctagaagc atcccttgtt
    23461 cataagaaga aagaagacgt atcaactcga tctttcgttc ttcccctccg ctccatttcc
    23521 gctctgaagt ttcttccagt cttaactgga aatgactggc taattccaaa tttaggtcct
    23581 ttgcttcagc gagataggtt aattgaggca ggtagttgaa ggtgatctct gtttggattt
    23641 tcccatgatc gggaaaaagt tcaccggcta ttagtttgag caaagtcgat ttaccgacac
    23701 cattttctcc tatgatccct atacgatctt gctgatagac acgaagagaa tcaatagata
    23761 gttctagctg gttgccaaag ctgacttgta gatttgttat atttactgct aaattttcca
    23821 tatgttttac actcctattt catgtaaaac aacaattatc gatactaaaa aagagcagaa
    23881 aaatctgttt gcaaaaaaac tgcatcacat cttttcctac tctgaacgaa ataactatga
    23941 ctaaatcacc aaatctgtca tagctttcac ctattctact gtcgttcgaa aaaatggaca
    24001 tactccatcc atgaaattgt tgtttataca tgtcttttga atttatttag ttgttattca
    24061 aaagttcgaa acgtaatttc atcgatgcag tcatttttcc gacctccttt tttattaggt
    24121 taccacacac gattcaaaaa aagcaatagc tactgaatag taactgaaaa ccttttattc
    24181 gaacgtagtg atcttttctg gtgctaaacg aacagactcg taaccttctt ctaccgcttt
    24241 tcctattgat aaaataataa ctggaacata acggtcttta tccaaaccaa atgcctctgc
    24301 caactgatct gcttcgaatc caccaattgg atttgtttca tagccatgat cacgcgcaac
    24361 taacatgaat tgcatagccg ctaagctaga atcgactttg actacatcgt tcatttcttc
    24421 tcttgaaaaa ctcttgtaat aaggaatgat cgcagctaat tgttgatctc tgacttcttt
    24481 tggcatcttc cctgattcgt aagcttggtt atagatttct tcgccatatt cgtaacattg
    24541 catatcgcca aaaatcatga ccattgcaga agaagtatca ttttgtctcg tattgaaacg
    24601 gatcaaagga cgcaagattt cttttgcttc ctcgctttca gcgaccacaa agcgccatgg
    24661 ttgcatattg acagaagaag gtgcagtcgt cgcttctttg atcatttcca gcatttcttc
    24721 gtgtgggatt ttgtatgttt catcatatac gcgaacggat cttctaccag tcataattgt
    24781 tgaaaaatca tttttttgtt ccattattat taacctcttt cgtttctgtt gttatattgc
    24841 aaagttgggt attcaattta tctaaaagtg ttagaaattg tttcatttct acatcgctga
    24901 aagccgggga tagcacggaa cctgtatctt tatggtccat ttcacaatgc tcgattttct
    24961 tctgggcttt ttctgttagc gctacataca cttcccggtt gttctgagga tttctctgac
    25021 gcgaaacata gccatttgct tccaatacct tcagatgacg agtaactgct ccttgatcaa
    25081 tctgcaactt ttcttgcaaa ctgctttgta gcaatggatt ttcttctttc agtctcatca
    25141 tgatctcgta tctagtaaga cttataccga tattttcttc aaagatctgg acgatttttt
    25201 gatcggctaa tttgatttga tataaaaggt gactaatctc ttctaaagac ataatttcct
    25261 cctttctaat aattgactcg tcaacgattg acttgtcaat aatagaataa aaaaagaaaa
    25321 aagtcaaaga atcagctgtg taataaaaaa aacgctctca atagaaaagt atagagaaaa
    25381 gaaatttttt gtagaatgaa cggaaaaaat gataaagaaa gcacaacgct aaaaccaaga
    25441 ttattgattc ttatcaaact atagaagata gctcttctgc agtagaaaat tttacaccgc
    25501 caaaaatttc cgaaatcgcc ttttgcaaga atcccgtcga atattagaaa ttggatcagt
    25561 atgcttaaac agtaataaaa aagttatgac aagatgttgt cacaaccttt tttgagtaaa
    25621 cggtgttcaa aaacttgcat ctgcggtaat aagttcaacc aaacaaaaaa catgaaaatc
    25681 tgtttttgtt tggctgttct tactacttga tgcagggcag ctttttcact acctcttttt
    25741 aataaccata aaatcctatt ccttcatatc tccagcccat cggtgttagc caatctcttt
    25801 ccttagcact caaggtatag tgatgggtac cagttttttc atttggatta aatagtcgat
    25861 aaattggctt ggaaccacca gaataaaaac ttagaccttc ataacgccaa ccgatttttg
    25921 ttaaagagtt gatctcagat tgtgccattg tgtaatgatg gtctccactg taaggattgt
    25981 ataaacggta gacaggttgt ccgctgtttg gtgcttgcca agcaatccct tcatattccc
    26041 agcctacagt tggcagataa tttttttctc cggcattcaa tgtatataaa tgttctcctg
    26101 tatgacggtt atacagacga tacatcggag aagttgaagt tggtgctgta tcatactgtg
    26161 tcagattata ggtttcgatt acattgttca atgtagaacc ataattaggc gcagtggcat
    26221 aacgacctgt tagccaagcc gtagcatctt tgtaagagtt cgtattgctt ttccatgcac
    26281 cggaatagta gtaaacgcct ggctgaaaag aagtcgtctt caataccgtt acgttatctt
    26341 gcaatgattc tttgtacgaa ggatattttc taaaagcggc attcaccgta atccattgcc
    26401 cattcacata ttccgaagta ggcatcgtaa cagattggcc attatagctt cctttaatcc
    26461 caaatagatt gtaattaggg gcagaagcca aagtactgtt cccccagcca ctttcaacga
    26521 tggcttgagc aatcatgact gatgcatata agtcattggc acttgccaaa ctttgtgcag
    26581 ttgaggcaat actatcaatg aacgcttgtt gagaattagc agatgagcga gcataaaaag
    26641 catagccagt atagttttcg aattcagaca aatctgcttg atgggtatgt gtcaatgttt
    26701 gcccatctgt caattggttc gtgatttctt ctgcattctc tggattgttc tgagcttctg
    26761 ctgatgtact gtcttcggtt tgtgtttgtt ctgttgtttc agttgttggt tcttcggttg
    26821 aaattgtatt ttctgagaca gtagctgact gcaaagtact ctctgtatcg ttttcaattc
    26881 ctgtttctga agaactcgat gtctgagttg cgacagtttc tgtcgattct tcagctagca
    26941 ctgttgtgtt ggcaactgct agatttccta aaagaataac gctaaataat aaattctttg
    27001 ctttcatctt ttttctcctt tgaattatga aacttttcct aaataacagt gtaactaaaa
    27061 aagtaaaagc aagtcaatgt ccatatttaa ataacatttg tttctttttg ataaatttgc
    27121 tttttctaca taaaaagacg taaatgaaga atattcttct ccattcacgc cttaaattga
    27181 tggtcgtttg aataactatt taacaagtaa ttctctttgg atattatccg ttatggatta
    27241 tacttcctgt gtcgtcggac ggataatcat gttactgaca gacatccggt ctggcgtttc
    27301 tatcgcaaat gctacagcag aagctatatc ttcggaggtc aatccccatt ctttttgcgc
    27361 ttcatgaagt tcaagagcag cctttttgtc gctgatggtt tgatataatt cagtttgaac
    27421 agctcctgga gagatgatcg tagatttgat gttattttgc cgttgctctt gacgtaatcc
    27481 ttccatgatt gcacggacag caaattttgt tccgcaataa acggcggaat caggataaac
    27541 aacatgtccc gccacagaat cagttgaaat gatatgccca gatttttgtt tttccatgat
    27601 tggaagtacg gcggaaattc catttaacac acccatgatg ttgatatcaa gcatattttg
    27661 ccattcatca cggtgacctt caactaatgg agcggttggc atgattcctg cattattgta
    27721 caatacatcg attcgaccga acttttccat cgctaaatcg ataactgctt ggacttgtgc
    27781 aaaatctctt acatctgctg tttgaatata tagctcagct tctggtaaag attcttttat
    27841 tgcttttagg cgatcttctc ggcgtgcagc tataactagt ttagcccctt tttctgctaa
    27901 tagcttcgtt gttgcttcac cgattccgct tgaagcgccc atgatgacaa tcactttttc
    27961 tgataaatca ttcattctat tctcctcctg ttttgaaaaa tctcttgctt atactcacta
    28021 tctcttattt catcttactg ttcaaataat ccgttcgaca ggtttgacga ttttggcagg
    28081 gattcctgca acgatggtat tgtctgggac atctttcgta acagttgcat tcgctgcaac
    28141 aatggcattc tcgccaatcg ttacgcctgg cagaatagtt gctcctgctc cgatccaagc
    28201 atttttcttg atgtggattg gctttactga caagcctctt cgttcttttg gatcggttag
    28261 atggttcacc gtgatgaggt tgacacgagg accaatcaat actgaatctt ctaaacagat
    28321 gccacctaag tcaacgaaca tcacttgacg attgataaag acatttttcc ctatactaat
    28381 atgtttgccg aaatctgtat aaaaaggcag cagaacagtc accgtatcat ctactttgtc
    28441 actaatgata ttttctagcc gtttgcggat atctgctggt tcatggtacc cagtattcag
    28501 cgctgctaaa ggctcttcgt tttcagcttg gatctgatgg atcacttcaa agagcggatc
    28561 cgttactcga atctctttat tgacgatttg tttttcgatg aggcttgtca ttttgctccc
    28621 tccttttatc cttactttat aacggacggc ttatcatgtc taatacttat aaaacatact
    28681 taactattcc ttttaactat acttagtggt atgctttttt aaaaacagga gggaaaaaat
    28741 ggatattcgt atactgacgt attttttagc agtagctaga gaaaaaaata tcagccgagc
    28801 agcaaaatct ctgcatatca cgcaacctac tctctcaaaa caactgaaag atttagaaga
    28861 agaacttggt gtgacacttt tcactcgggg ctcccgagag atacagttga cggattctgg
    28921 tcgatatctt tattcaaaag gaaaagaaat cttgtcgttg gtagaaaaaa cggcgaacaa
    28981 tttaacaaat gacgcaatca tctctggaga aatctacatt ggcggaggcg aaactaaagc
    29041 aatctctttc atttctgata gccttcattc gttgatcaag gcgcatcctg atatccagtt
    29101 tcatttatat agcgggaatg cagatgatat catagacaaa ctggatagcg gattacttga
    29161 ttttggactc gtaatcgaac cgacaaacaa acaaaaatac cagtcatttc gactgccagc
    29221 ccaagatcgt tggggacttt tgatacatcg aaatcatcct ttagcaaagc aaaccgtcat
    29281 ccatccgcag gaactgaaaa ctgttccttt gatcatttcc cggcaaacgt cagtggacag
    29341 tcaactagct gaatggctag gtttcagcat agatcagtta tcgatcgtcg gaacatataa
    29401 cttattgtac aatgcttctt tgatggtcga taacgggact tttggtgcat tagctctaga
    29461 tgggatcatc aatacttatg ggacggaaaa aatattcatc ccactcgatc cgccattaca
    29521 agcttcactg aatctcattt ggaaaaaaga ccagcctctt tctcctgctg ctcgtaaatt
    29581 tttagatgtc ctaacggaga aatacagtta agtagagttt ttttccttcg ctttcagctt
    29641 tttaacaacg aataagataa gcaaaagatc caacgattaa ataacataag tttcttaatt
    29701 tatttcttcc ttttatttct gataattcca gtaaattact ataattataa aaaaagaatt
    29761 gacaaaacat aactgtctat ataaaattga ttacgttaac aataattcgt ttcatgtgac
    29821 tagatcatac taggcatgaa aaatctctga ggaacatggt ttccctagga ggtttttcgt
    29881 gtctattttt gtttggccgc aatcaactgg tctagcaaac gtgaaaacca aatcttcaaa
    29941 aagggtgcgc gcatgaaaat caaaaagatc tttaatcaaa atgcagtatt ggtagatgac
    30001 ggtggacagg aaaaagtagc tattggaaaa gggattggat ttgacagaaa aagaaatgat
    30061 ttgatttttg atcgtgatgt cgaacggata tttgtcatgg aaccagaagg tcaaatcaag
    30121 ttgcaaaatt tacttaatca gattgatgaa cgctttttat ttgcagcaga aacgatcatt
    30181 gatcatgcgg aaactgtttt gatggaaaag ttgaatgaac atgtattgat cgctttgacc
    30241 gaccacattg ctttttctgc ggagaacatt aataacggta tcgtcatccg caacaaactt
    30301 ttaagagaaa tcgaagtgct ttacagtgat gaattcagta ttgctcaatg ggcagtagag
    30361 tatctgacaa aagagcttgg gattccatat acttatgacg aagcaggata tatcgcaatc
    30421 catattcaca gcggacgaag cggtcggaac aataaccatc gcagtatccg tgaagtgacg
    30481 attgtatctg acatcatcca tttagtggaa tcagaattgg aaacagatat ccattcagaa
    30541 ccattttcac tgaattattc aagactggtg aatcatttac gactgcttct acaacgaacc
    30601 catgcgcaac aatatgctgt tttagacacg gaaatcgtag aaatggtcaa acgaaaatac
    30661 ccagaaagct ataagatttc taaaaaaatc cgtgtgttgc tgaccaaaga ataccagctc
    30721 gcgatcacga aagaagaact gggttacttg gccatccata tcgagcgttt aagaagcgca
    30781 atcgtacaaa caaatgttaa caatcatgag gaggaaaaaa attgaaagca tatctacaac
    30841 gaatgggacg ttcccttcaa cttccagttg cggtcttacc tgctgctgct ttactagtag
    30901 ggatcggaaa ctggtgggca tcttatagta acgacattgt cgctcatttt ttacaagctg
    30961 gcggtaacgc agttttaggt caattacctt tgctgtttgc cgttgggtta gcactaggga
    31021 tgtcagtgga caaatcaggt gctgctgcct tggcaggttt ggtcgcattt gaagttccag
    31081 taaatgtttt aaaaactgat tcagtagcta ctttattgaa tatcaaacca gaatcagttg
    31141 atccatcttt tgctcagatc agtaacgtat ttattggtat tattgccgga ttgattgcgg
    31201 cagctcttta taatcgtttc cacgaagcaa aattaccgat ggctttatct ttctttagcg
    31261 gcaagcgtct agtgccaatc ttggctgcct ttgtcatgtt gctgatcagt gcagtgctac
    31321 tgctcgtatg gcctccggtt tactctgctt tagtttcttt tgctaaattc attttgagtt
    31381 taggctgggg tggtgcaggt ctttatggct tcttcaaccg tttactgatc cctacaggat
    31441 tgcatcaagc aatgaactcc gtgttctggt ttgacgtagc aggaatcaat gatattggta
    31501 aatttttggc ttctgaagga acaaaaggcg taacaggaat gtatcaagct ggatttttcc
    31561 caatcatgat gttcgggctt ccagcaggag catatgctat ttacagaaat gcacgtccag
    31621 aaaacaaagc aaaaacagct tccttgatgc tagcagcagc ttttgcttca ttctttactg
    31681 gtgtgacaga accacttgaa ttttcattta tgttcgttgc ttggccatta tatgtcttgc
    31741 atgcaatttt cactgggatt tctttagcag tgagtgcatt cttccattgg acagcaggat
    31801 ttgcctttag tgccggattt gtcgattact tcctatcttt gaaaaatccg attgcgaatc
    31861 agccattgat gttgatcgtc caaggattag taacagcagt gatttactac tttggcttta
    31921 actttgctat caaaaaattc catctgatga ctcctggccg cgaggaagca gatctttcag
    31981 atgacgatac tgcaacgact aattcagaca ataaatatgc ggcacaagca agcaaaatct
    32041 acgctgcatt aggcggagcc gacaatgtaa cttcgatcga taactgtact acacgccttc
    32101 gtttgcaagt caaagataca ggcctgatcg atcaaaataa aatcaaagcc actggcgtac
    32161 ctggtatgaa gatcatcgac ggaaaaaatg cgcaagtcat cgtaggtaca gaagtacaat
    32221 tcgttgcaga tgagatggca aaactacatg gtggtgctgc agcacgacct gcacaaacaa
    32281 acaccgtggt taaaacagaa acatttgccg gtgaaacgat cgaacaagat atttacgcta
    32341 tcgcaaatgg gaaactgatt ccaatcactg aagtttcaga tgatgtattc gcggaaaaaa
    32401 tgatgggtga cggctatgct gtattaccag aaaatggaga aatcttctct ccaatcgctg
    32461 gtacgatcac aaacattttc ccaacgaaac atgctgtcgg aatccaaaca gatgctggta
    32521 tcgaagtctt gcttcatatg ggcatcaaca ctgttgactt aaaaggcgaa ccattcactt
    32581 tatatgtaga agaaggtcaa aaagtcgcaa gaggtcaatt gattgcttta gttgatcttg
    32641 ccgctattca atctgcaggc aaaaacacgg atatggtcgt tgtattcaca aatggcgaca
    32701 aagtccaaag cttagaaatc gaacccgcac gtgatgtaaa agcaaatgat aaaatcggct
    32761 ctgtttccaa caaagcctga gccatcgtct tccccagact tttttatcga taaacaaaca
    32821 ggagctgaga caaaaatctt gtctcagctc ctttttccga ataaaaacgg cgttctcctg
    32881 ccaactccgc ggtaataagt ccagctaaac gaaacatatg aagagctatt ttttatttga
    32941 ctcttcttat tacttgatgc agaacagctt tttcacaaaa tttttcttca tactcaaaac
    33001 taatcggctt tttcacaacc tctttataca ttattcctga tccaatattt cttttaatgc
    33061 tgcttcaaga gaagttaatg gttttcccaa tactttttct aaatcatcag aggaaatagc
    33121 taactgatcc tttttaatat catgttggat cgctaaaaac atgtcagctg cttcttgagg
    33181 gaatccggct tcttttagat tttcgacaaa cgctgcatca cttgcttcaa taatttctaa
    33241 ctctttacct gtcgcacgct ctaatgcctt ggctaattct tcatagtcag ttagattgcc
    33301 ggataattct aagatttctg gagagtcact ttctactagt acttttgctg cagcttctgc
    33361 atattcacgt ttcaatgccc aacctgtttt tccatttcct gcagcataaa cgaattgacc
    33421 agtttttaaa gcctgcccaa tgataggtaa ttcattctct acataccaat tattccgtaa
    33481 aaatgtatgg gaaatacctg aatccttaat cattttttca gtgaattggt gatctggcgc
    33541 taatacactg atcgagtgat cggcatctgc aaaacttgta taagcgatat aagatacccc
    33601 agctgcttgt gctgccttca ctacattctt atgttcggct tgacgatttc ctggaacgcc
    33661 agagacaaaa agcaatcgat ctattccttt caacgcttct ttcattgagt ctaaatcatc
    33721 ataatcacca atacgtatat taaatccagc ttctttcagt gcttctcctt tttcttcact
    33781 acgtactagt acatagattt cattttgtgg aaccatcttt tttaagaatt ccagcgcata
    33841 accaccaaaa cctccagttg ctcctgttac taaataatgc atatttgcca ctccctattc
    33901 tttttatttg ttaagattta cagctgtaca tttataaatt acacctttat cataaagagt
    33961 gtggtaagat aagtcaagca aaaaaaatga aaggatgaca actgatgaag tattctctgc
    34021 aatttagtga tgcaatccat gtacttgcct atattgaaat gtttcaagat acccgatcac
    34081 tttctagtga gatgattgcc agtagtatcg aaacaaatgc agcaaatgta cgtaaaatca
    34141 tgagtcagct caaaaaatcc ggtttgatct ttacaaaaac cggaaaaccg cagccttccc
    34201 ttgccaaaag tccagatcag atttctttac tagatgtcta tcgcagcatc gaaggtaata
    34261 cacagcttat ccaagtcgat ccaaaaacaa atccaaattg cgtcgttggt gcgaatatcc
    34321 aagaagcttt agaagaaagc tacgctcggc tgcagaaaaa ggcagaagaa gaaatggcta
    34381 agatcacact tgatagtctg atacataaaa tagccgtttt ggaaaaagaa aaaagaccag
    34441 aaaacacagg cctcgtagag agatttttat aattctctct actgtgcctg catttttctg
    34501 atcggaatcg tctacataaa ttcttttatt ctccggggga caaaagcctt gtcatgcccc
    34561 tttttcctct ctaatgctca ggtcaaagcg ctgaagtcat gacgtcttga taaacggtgt
    34621 tcaagtagct gaccctcggc attagttctg aaaaatgcaa aatatggaaa gctatttccc
    34681 atttttcctc tctaatgctc aggtcaaagc gctactttca cgacctctat attatcatgg
    34741 tttattgact accggtatcc agatttctcc catatatttc ccgttggtaa ctcccatttc
    34801 gactacagca tttggacccc caacataggc gtaatcttct acttctctta aaacatgccc
    34861 aaaggccgta ttcgtcaaat catttcttaa ttcttcaggc gattctgcta aagcgcttac
    34921 aacaagatac gttccttttg ggaatgggat gattcgcgta gtgtttggaa catcctgact
    34981 cgtttgaaca ccgacatagt acatcatttt cccgttatag ggttcgttga ctgtaaagaa
    35041 ataatgattt tcagcagctt gtttcaatgc atctattgtt ccattaccga taactttttg
    35101 ctcaaatgct tgtttctctg cagccaaacc gacaaaatcc gtattgctac tcttcaattc
    35161 cacaccaata cccgtcaccg taaaatctgg tttttcttcg atcctatact ttgccatttc
    35221 tcccaccctt tctattatat taagttcatc ttacataaaa ttaaagggaa atgacacgat
    35281 tacgctcttt tttctaggga tatttttatt ttttaactat ttttttgctt tcactgcaat
    35341 ccaaatctca ctgtaaacat tactgaaatc ctctccccat tttgtaaaag agatctcggg
    35401 agcttcgact aacccataat ctgaagaagg aagccattct gcatagattt tcccccaagt
    35461 atcttgtaaa gtttggggaa atggcccttt attaggaaaa atggcccaag tgtgttcttc
    35521 tatagcaagc atttctaaat cagcgtacgg attttctttt gtcgtagcaa agccaatcat
    35581 gtgcatcaaa ctgccctttt cttccaagcg gtctccatca aatccataag aaacgttgat
    35641 tacttgattt ggatataaat ctcctaactt atgcatctct tccctttgag tctccgtgat
    35701 cgtctgcgct aacttttgta tttcttgatt gactccttca aatttgacag gaactttttt
    35761 cgccacacct gccacataaa actgttcttt cttttcgata cgaaattcca tcgattttcc
    35821 tcctctgaca tcaataaaaa atgtcaagcg tggaaaagat ttttgtatgc cgtatctact
    35881 gatttctgaa ggcagatatc ctgaccaatc acgaaaagct cttgaaaaac cttcgactgt
    35941 ttcatatcca tatctaaacg ccgtttctgt cactcttgat ccatctagaa gatcgcgatt
    36001 agccaaagcc aatctacggt ttttgatata gtccattagc gacatccctg caataaacgt
    36061 aaagatgcgt tttaattgat attcagagat tcctgtattt tcggctatta ttttatagga
    36121 aagtccttct gtcaaatgtt cttcgatgta atcgatcaat tggttcagat ctttaagcat
    36181 atttatcttc ctccttgatt gcctttttat catattatca aagaaagtca attacttgat
    36241 gaatcaggaa ccaatttagt cagatgtaga aaatgttgta tggatctaaa gaaaattctc
    36301 aagcagcgtt tcgtaacgga taattggtat tatctgccta tctatgtttt acctttagaa
    36361 aaataaattt tggggaagac agattctaag taaacaaagc ggtaaaaata tcttttattc
    36421 ccttagttaa tccgcttact acctactgta caaagcaaat tgttgttcaa actgctgatc
    36481 agaacgctgc ttggattttt ctttgcgata catcatacct cttttatcaa ttggaacaat
    36541 tatttatttc taacaaaatc acttcatttt tgtcagataa ataaaaaaca aaaaaagttc
    36601 catctttaaa acacgctatt cttttacttt ttctcacatt ctctaagaaa agaaaaggag
    36661 aatgaatttt tgacaattcg agcaaaagtg attatcaaat atgtaaaatc agctactttt
    36721 ttcttaaacg tttctttata ctaaatataa ataagtgttt catgaaagga gaactagatg
    36781 aataattggc ttgagtattt tcctgaaaac gttttggaac gcggttacag ctatcatctg
    36841 cacggttttg tccgtcattt gaactataca agcaagtact tgtccgcaac agtttctggc
    36901 acggaagatt ataaggtagt gatcacttgg gatgaaaaga ccaacatgac ttgtgattgt
    36961 ctatatgcca tagaaggaaa aaaatgcaaa cacatggctg ctgtcttatt cgcttacgaa
    37021 gaacgtccaa taaaaaaatc caactactca ctttccgaac tctcgtccct tgtttcctcc
    37081 gctagttcct ctcttgtacg ggagctttta acagagattc ttatagaaca tccgcacttt
    37141 atcgaacgct tcaaagttaa gatgccattt catgcaataa attattcaga taaattaaca
    37201 actatcatcc ataaatacga tcatattatc aagaaaaaca aaaacaggaa aacagcgaag
    37261 ttcatcatgg agatgcgaaa gttcatccaa gaagcagttg aatcgcttat tcagcaaaat
    37321 gcctatcttc ccgcattcga attgatcaat gaagtgattg ctactctcga aacattctat
    37381 tgggagcctg aggatgaacg aacccttctt ctgatagagg actgttatta tttatggaaa
    37441 gaattattag cagaagcacc tcatgcagaa aaaaggcaaa tgttctcttg gttcgtctgc
    37501 caagtcgatc atactgacgc ctcttattca aaacgctact ctataaaaat cctgaaagaa
    37561 gactttcggg aaaaagaatt ttctaaccaa aagaaaaaaa tagataaaaa actaaagaaa
    37621 cggtaaagaa agacgatttc aatgaaaaat agcaagtaaa cgataacaac catcctaaat
    37681 ccacaagaaa tacagcagac cttgctttaa aaacacgaat catgcaaagg atgaagtaaa
    37741 gaagacttaa cgcgtaccca atattagtca tttgactatt aggtacgcgt tttgttttat
    37801 aagtcatgac aaatttcctt cgttgctgga tagattttcc ggcattgttg atagatttta
    37861 tgatactttt ctacattttg taagagaggt tccgctgcat ctgtatattg gataaaattc
    37921 tgacaacaac tttcaggtgt gtcgaaccac cctaacccta gtgcagcaat cattgttgcg
    37981 cctaatgcag gtccttcctc catacttaaa caatagaccg ttgcatcgaa aatatcagct
    38041 tggatttgta gccatgcaag atttttcgcg cctcctccca ccgaaacaat acgtgtaaat
    38101 ctgcgacccg ctagttcttt acgtttcaat cccaccattg acaaatgctc attaaaaatt
    38161 tagaatattc acaagtttaa cgacaattat gaaaatttat gtcgtgctaa aggagttttg
    38221 ttattggatg aaaagttaga tataaaaact gcgatcaaag acacgttacc taccgttttc
    38281 ggttatatcg gtattggact tgcatttggt atcgttggga aagctgccgg atttcatcca
    38341 ctagtcgtca cgttgatgtc cctgctggtc tatgctggtt ctgcccaatt tatcacagtc
    38401 agcatgcttg ctagtcacag cccattgctt tccatcgttt tctcgacctt tctagtcaat
    38461 tcccgaatga ttctgatgag tatgacgatt gctccttatt tcaagaaaaa tcggctgctt
    38521 caaaatctgc tgattggcac attgctgaca gatgaaagtt tcgcactggg aatgaataaa
    38581 ttgaactata ctggacaaaa gttaaacttt cgttggatga atacagctaa ttggatttct
    38641 tatttgacgt gggtgggttc ttctcttgtt ggcgcacttt tagggaattt tattactgat
    38701 ccgaaaaagt ttggtttgga tttcgccatc gtcgctatgt ttatcgggtt actttatctt
    38761 caagtcattt ctgatagaaa cacctctaaa cgattacagt tgatcctgat tggtctcact
    38821 ttgattctgg tatatgtcgg actgattttt atcccaagca acttagtgat tgtagttgta
    38881 acactgattg ggtgcggatt gggggtgtgg atcaaacatg ccttcttttg agtttacatt
    38941 gctgactatt ttaggttgta ctttagcaac ttggctttct agggtcctac catttgtctt
    39001 actgaaaaaa ttcgatttac cgcaaccgtt gcttgagtat ttaagttttg tcccgatcgt
    39061 cattatgtct gctttatggt tcagcagtct atttacccaa aatatcggac atctgccgca
    39121 gatcaatctc gaaaatgctt tggcttcagt tcctacactt ttagctgcta ttctttcaaa
    39181 aagcttattg gtgatcgtct tggcaggaat tctttccctt tcgttgattc gcttattata
    39241 aataaaaaaa caagagctta tggcaaatta ctgtcatgcg ctcttgtttt tatcaaagtc
    39301 aaataagaaa aaaatcggcg tattcttgct tatccgttga acggaccact aacaacgact
    39361 tttcctaaca tatgtcctgt ttctacgtct ttggttgctt gtttcaagtt cttcgcattc
    39421 atcccatctg aatagacctt gctcaatgta ctgcggataa tcccttcatt tgctaaagca
    39481 gcaatgtgcg ctaaagcttc tccttgcgtt tcgatttcat agttatagtc tgttttagcg
    39541 aacatatact cccagtcgaa gctgatagat tggtttttcc aaacacctat atctaacggc
    39601 tctttgatcc ctacgatcgt tcccacatgt ccaaatggtc ggacaaggtg cttgatttga
    39661 tcaaagtagc gggtaatatc gaataataca gcgacatact ctacttgatc gattcctaat
    39721 tcacgaaaac ttttacttaa gtcgtgatga taatcgattg gatagtctac acctgttttt
    39781 ttcagccatt caaaattctt cggacttgca gtcgcgtaga cttctaggcc agcccaatgc
    39841 gctagttgat tcaaaatcga accgacgccc ccggatccgt tgatcactaa gatttttttc
    39901 ccgcgattgg cattttcttc tggagttaga ccaaattttt caaataacaa ttcataagcg
    39961 gtcaatgagg tcaacggcaa ggctgcagct tcttcatctg aaagattttt tggtgcaaga
    40021 gcgacaatac gttcgtcaac tagttggtat tcttgattgc ttcctgctct tgttgttgta
    40081 cctgcgtaaa aaacgcgatc gccgacagaa aatttttgca cttcgtcacc tacagcaaca
    40141 actttcccga ctccatcaaa acctaagata cgtaaagcat ttcttatccc gttatcttgg
    40201 cgaagtttcg tatcgacagg attaacagat actgcgttga cttttaccag aagatcccgt
    40261 gaactgggta caggtatctg ttctttttca tcaataaagc tttttggatc atcgatcggc
    40321 aacccttcat aaaaaccgac taccttcatt aattcctttt ctgacatacg aattcctcct
    40381 tagcttcatc aagttggagc atccatacag tgttcatttc aatatatata gtatgctatt
    40441 tgttccacct tttttattca tcataattat tctcttactt aaagtaaact atttgcactt
    40501 ttttggttaa aaaagaggtt gcgacaagac ttttgtcaca acctcttata aacggtgttc
    40561 tcctaccaac tcttcggtca caagtcaaaa tattcatcaa acatgagaag ctgttttttg
    40621 aaattgctcc ttgtgactca gagttaaacg gcaggctcac aacctctgat ataaacggtg
    40681 ttcaatcaac tgaccctcgg tattagctca aaaaacgaca aaatatgaag agctattttt
    40741 gtttggctgt tcttattact tgatacagga cagctttttc acgacctcta attcacggtg
    40801 ttcaagcaag aaatcattgt tcactgaaaa gcgttggatg ttctttttca aaggtctcta
    40861 agatctctcg tacttcatca gtagatttcg tattcatcaa ctgaactcgc aagtcatttg
    40921 ctcctggaaa tcctttgata tagattttaa agaaacgatg tagtcctgtg atggaacgcg
    40981 ggatgatttc tgaataatga tcttgcagat ctagttgcag acgaagtagt ccgatcagtt
    41041 ctttttctgt atgttccctc gggtcttttt caaatgcata aggattcttg aaaatccctc
    41101 ggccaatcat gatgccatca acgccatatt tctcggccaa ttcttctcct gtctggcgat
    41161 cggggatatc tccattgatc gtgattagtg tttgtggagc gatttggtca cgtaaagcaa
    41221 tgattttagg aatgacttcc caatgtgcat ctactttact catctcttta cgcgtacgca
    41281 aatggacaga aagattggct atatcttgat gaagtaggtg actgatccat tcttccatct
    41341 cagacatttc cgtatagccg atacgcgtct tgacgctgac aggcaaaccg ccagctttag
    41401 ctgcttcaat caattgtgcc gctacttctg gacgaaggat caagccactg ccttttccac
    41461 gttctgctac attagggact gggcagccca tgttgatatc gattccttgg aagcccattt
    41521 ctgccatagc aatgctcatt tctctaaaaa attctggctt gtctccccaa atatgggcaa
    41581 ccatcggctg ttcatcttct gtaaatgtta atcttcctcg tacactgtct tttccatctg
    41641 gatgacagaa actgtctgag tttgtaaact cagtaaagaa gacatccggt gctgctgcat
    41701 gctttacgac atggcgaaat acaacatctg ttacatcttc cattggtgct aaaacaaaaa
    41761 aaggctttgg cagctctttc caaaaattag tagtcattta tttgttactc tccattctat
    41821 caagcagtga agtcgcaatc tttctcaaaa acattgtcat tatactagga aacaaaaaaa
    41881 gaagcaaagt gaacagctta aaagatgttt ctgattgagc aaaaaaaaga ctgtgacaaa
    41941 aatcttatca cagtccattt tccggaataa atggtgttca aaagcttgca tttgcggtaa
    42001 taagtccaac caaacgaaaa ataggaggag ctatttttgt ttggctgttc ttattacttg
    42061 atgcagaaca gctttttcac aacctctcca ctaaacggtg ttcaatcagc tgaccctcgg
    42121 tattcgctca aaaaacaaca aaatatgagg aactattttc ccgtttgttt ctctaatact
    42181 caggtcaaaa cgctgatttc acaacctctt ttctaacttc tcaatcctct tcctttattg
    42241 atcaaatacc aagtaacggc gtacaaaaca acgatgaata acacaagtac gatcatcgat
    42301 gttgctactg gcacatcgat cgttccaagg aatccgtaac ggaaaccgga gatcatataa
    42361 acgatcggat tgacttttga aacagcttgc caaaagggcg gtaacataga gatggcatag
    42421 aaaacgccac ctagataagt taacggctgc aagacaaatg taggcacgat cgatacatca
    42481 tcataagact gagcaaaaat cccgttgagc agtcctgcta atgaaaacaa gattgccgtc
    42541 atcaataatg taatgatgac catgaaccat gaatacacat gaagtggtac aaagaacaag
    42601 gaaatgatcg ttacgagaga gccgacgaga atactgcgac ccacaccacc aatcacaaag
    42661 ccccaaataa tcacatgcgt aggaacaggt gctaccaaga tttcttcgat atttttctga
    42721 aatttttgag agaagaagga cgaagaaaca ttggcatacg aactagtgat agcagacatc
    42781 ataatcaaac caggaacgat gaattccata tatgaaaaac cacccatgtc accgatccgc
    42841 ccaccgatca tttttccgaa aataacaaaa tacaaggatg tcgtgatcac tggcggaacc
    42901 agtgtttgga cccatatacg caaatagcgg ttcgtttcct tgatagctaa actttttaat
    42961 gcagtaaaat aaagattaaa catttttctc ctccgtatga ttattttctt ctgtgatttt
    43021 caagaacagt tcttctaaac gattcgactt attcctcata gaaagaactt tgattccttg
    43081 tcttgtaagc tgatcgaaga ttccgttcac cccttgattt cgttcaactt cgacagcgat
    43141 tgtctgctca tcttcaaatg tacaagcata tccttcaatc acaggtttgg tatcatatgg
    43201 cgctaaatca aagataaatg tttcaaattg cagttttgac agaagctctt tcatacttgt
    43261 attctcgatc agttcacccg attggatgat tccgatgttg cgacaaagca tttcagcttc
    43321 ttctagataa tgcgtcgtca aaatgatcgt tgtaccattt tcgttcaatt ctcgtagaaa
    43381 ctcccacatc tcgcgtctca actcgatgtc aactcctgct gttggttcat ccaagatcag
    43441 caaacgtggt tcatgcatca atgccctagc aatcatcagt cttcgcttca ttcccccgga
    43501 aagcatacgt gcacggacat ttctcttttc ccaaagattg gactgcttca aatatttttc
    43561 gctgcgtttc atcgcttctt tgcgagagac accgtagtag cctgcttgat tcacgacgat
    43621 ttgctggacc gtttcaaaag ggttaaaatt gaactcttgt ggaaccaatc caatctgttg
    43681 ctttgctcga actagatcct tgtccagatc ataaccgaag acattcactt ttccagatgt
    43741 tttattgact aaagaagtaa taatcccgat cgttgtagac ttcccagctc catttggccc
    43801 taatagcgca tagaaatctc cttctttcac cgaaagatcg attccccgca atgcttcgac
    43861 cccacttcca tagatctttt tcaaattatt tatttcaagc gcatttgcca tttatttcct
    43921 atctccttat agacatgatt cttaattgta tcatggaata cagcagatga aaaaacaact
    43981 tggcttccac acaaaaacac acctgctaac aaagaggaga tgtgttttct agttcatgat
    44041 catcttcgat agaataacta tctaaacaac tctggaactt ttaaaatcat aaaagtaccg
    44101 acagcaacta ctacgatgac atatagaaga tccttataac tgaggttctt gtctttttta
    44161 ttcatgttaa gctttcctcc gtccttttat cgaaagcttt tttcttttat tttaaccgaa
    44221 ataaataaaa tggaaatagt tattttcaat ttctgttttc gctgtttgtt ttacggagaa
    44281 ctttgccatg gaacgtgcgt ctcgtcgctg atgctaagtt ttttcgataa aagctgctag
    44341 aaggaagaca gcgtggttta ttttgtatcg tcttttgcta aatcttccaa gatcatctgg
    44401 acagcagact cgttcccatt gaattctgcc acttttttgg catcaatgta cttcttttga
    44461 taatcgtctt tttggaccca cgcatcatgc ttcgtttgat aaagttaatc aaacggatac
    44521 cactgacgct gttcatcgaa caacaaactt tctttttggg cctgttcaac tatggattta
    44581 ggataattca aaactttcag caaggcaatc gcatttcggg tccttgaagg tccttctttt
    44641 aatttgaaat caaaactgat tccttttcct tctgttactt gttcttcaaa gtggacattc
    44701 tcacaataat tctttaaaat ctctgtcaat tcgatatcat gtgtggcgac aaatgctaaa
    44761 gatggatact ccgcaagcca ccgaacgact gaagcggaag aagcaatccg ttcgatcgta
    44821 tttgttcctt ttaaaatctc atctacgaag cagtagcagc gttctttgga agccacttgg
    44881 tccagcaaac gtttgatcga tttgatttct gaaacaaaat aactgtcgcc ttcaaataaa
    44941 tcatcttcga ttgccatgga agtcaacaca tgtccaggtt gcagggtaaa ctgttctgca
    45001 atcgctgttt ggatcgtctg tgctagaata cagctgatgg ctatactttt gacatacgta
    45061 gattttcctg atgcattgga acccgtgact aatgtattct tctgccagtt gactggatta
    45121 actaccgctt cttttaacaa tggatgatag atatctttcg ctgtcacacc gccctcagta
    45181 aactctggtc ggcaagtgat cggcatatag gtgcgataat tcaaaacggc tgctgcaact
    45241 tccaattctc ccagcaattc ccacagcttg atggcttcag ttgaatgctt ttctaattta
    45301 gtgagaacga aatgatagga gataaatggc agcatgaaca tgatgttgac atattcaaaa
    45361 atcaattctg cttcactccc gctcttcatt cgaaatgaaa aacctaattt agggatgtct
    45421 cttaatggtt tagtttgctg tttcagctct ttttgaagtg gtgtcgtcat ttttgctaac
    45481 tgattggcgg ctgaaatcgt tgaaaccaaa taactcatgc tattcagctc tgtttctagt
    45541 gtttgtttct tcactagata atagaccgta ttgaatacta agccaccgat tgctagatag
    45601 attcctagta cttgccctaa aagaagcaaa cctacaccca ccagcggaag caagccaaga
    45661 aaaataaata aacctacgcg tccaatcttt ttactttttc catccgctag atattgtttt
    45721 gcaaaattat gatctttttt tcctaaacga gcgaattgat attggattct ttctctcagt
    45781 tgaggatttt cctgataaaa agcaattagt ttttctaact gataatcttc tccaaaatca
    45841 aagctacgta aacgctgata caacgcttct gaacctacac tagaataggt actgttgatc
    45901 ccttcaaata cttcgaacat atccaagtca taccaagtaa gatcgtcgat ttcactatcc
    45961 catgaacgga attttttctc cgtgagccac gcatccttca aactttcttc tttatcaaat
    46021 ctcggttgat aaggaatttt tccccaatta ctacgaactg tttcctttac tttcaaacgt
    46081 gaaaaaatgt ctaaaacaac taataaaata acaactgcaa taataattaa aacgataaat
    46141 tctctttggc ccatatccct ctccgttcct tgatttgtta gttttagtat agcactgttg
    46201 cgctatttga aaagggggta caaaaataac tgtcttcgtt acctaattcg tatcaatcac
    46261 accaaaataa aaacaccttc caaaaaagaa gatgtcttta aaacgagata caattttttg
    46321 tcattcactt caggtaaatt agagccaatt aaaccctccg caacttgacg gagatggaga
    46381 agatgccccc gtctaatcta gcggtcatct ctcctcccat tccttccacc aatttttttg
    46441 acaggtaaag cccgattccg ctagattccg tatttgtgcg gctggtattt tcggtataaa
    46501 aacgctggaa gactttttca acagaggttt gcgctcctgg tttgatagga ttggccactg
    46561 ttaatcgaat gaatgaatcc tcttttgaaa atgtcacctt tgcttgtcca tctgcataac
    46621 gcaataaatt ctgtatgata ttttgcagca ctcgcagcaa catgagcgga tctgctaata
    46681 cagttgtatt tatcgagtgt tcagcgaacg ctacctcgat atttttctca gaaaattgtt
    46741 cataaaaaga gaaaaatact tcttccacct ctttagaaaa agaaagcgta tggagatcca
    46801 tttctttttg cttcgtctct attcgagcaa gatcataaaa ctgatccgtc atactgagta
    46861 atcttgcaac agattgtttg atgatcgtta aatacttctc ccgtttctcc gtatcctcta
    46921 cctgttccag taattgtaca taaccgttaa tagcagtcaa aggagttctg agatcatgag
    46981 aaaggccgac gatcgatagt ctgatgtttt cttccatttc ttgaaatgct tgtccttttt
    47041 gttcattttc atccacccat gtattcagtg catctactag gtccattaga ttcttttctc
    47101 gaaattcgac tgaaagacga ccaccatatt cttgtcgttc agaaagagtg gcgattttct
    47161 tagttaatct cttgatttcc ctttgcagga agaaaataaa acttcccata aagataacaa
    47221 cgagaagcaa cagccactta atcaaatcta aaccccactc cccacaccgt atgaatatac
    47281 acagcatgtg cctttgctag tttccctcga agattggaaa tatgcatatt cactgttttc
    47341 tcactatcaa aatatggttc ttgccaaact gtttcataaa gatttgctcg cgaaaatact
    47401 tttttcggat gttctaaaaa aagcttcagc agatcgaatt ctcttcctgt caaatggaca
    47461 gccacaccat tcacatttac atccctcgac tctttgtcca gtaagatttc cttgtaatag
    47521 atgcgttgcg acagatcttt tacttggaca gaagtctgat gacgaagttg gatgccgatt
    47581 cgtaacaaca gttcttcgat atcaaatggt ttcgtcacat agtcatccgc tcctgctgcc
    47641 aacaaggaaa ctttatctct ttgatcgctt tttgctgaaa gaacgatgat cggaacattc
    47701 gatgtctgtc ggatttctgc taacaatgct tcacctgaaa gccctggaag catgagatct
    47761 agaaggatca aatcgacagg atatgtgctt aacaataatt ttccttccgt acctgaataa
    47821 gcatcaagaa gtttgtatct ctgttcaaga acctcctcca gtaaagaatg aacagcttca
    47881 tcgtcttcga taatcaaaat agtttccagc aggaacacct acttaatgtc agatttttgg
    47941 aaaataaaca ttcctagagc tgatgatacc acgattgtta ctagactgac cagaatcatt
    48001 ttcggtgctt gcgaataagt tgcttgttgc gcaaagtccg tatacatgag ccacgtttcc
    48061 gtaaaccact ccgttagaaa ttccaagcct ttgatttttg cgaaaacacc gggaacgaag
    48121 gccgttccca tgatataaag aaaagaaaga agcaaactga cactggtact tctagtgatg
    48181 aatgtcaaag ccactggaac agataatgtc gctaatatca gcagaagcaa tacaggaaat
    48241 acttttagaa cataatttcc atagtttttg attccctcaa cttcaccaaa tccgaaagca
    48301 agtgtgtaag caatcatggc tagccccgta atcaaaacca cgccaagtgt tgtgatcaaa
    48361 agcgtaacga gaagtttcga cacataataa gtcattcgat tccgtcctaa agataacgta
    48421 ttgcgaatcg taccgttttg aaattcttcc ccaatataaa agcacgcaaa tgctgcgaaa
    48481 gccagaagaa tccatgcagc cattttttcc accattaaaa tccctgttgc accgttgatg
    48541 actgtatccg ttgcttggtt taattcattg ccgtattttg tatgcatata ccattcgttc
    48601 caagaataaa aaagcggaag tacgaccatc agtcctagtg agacccacat tttacggctc
    48661 ttccatactt ttattttttc agcagttaat agattaaaca tgtggattgc ctccagttaa
    48721 ttgtaagaaa taatcttcca gtttttgccc aactaggtgg ataccatcca caggaacttt
    48781 tgcctctacc aatgcttgat tgatggctgc tacttggtca attccttcaa aaagctggat
    48841 aacacggcta gattggacaa aataatccct ataaccaaga gagtcgagaa ccgttactgc
    48901 ttgttccgtc gccgaagtgt caatttttat aaattgacga gattcttgtg ccaattcttc
    48961 tttcgataat tctttgatca agtgtccttc atgcaaaata ccgtagtgag tggcgatctg
    49021 tgacaattca tctagcaagt ggctagaaag caagacggtg atgccccgtt cttttaccaa
    49081 acgttggatg atttctcgca tttctgccat ccctgctgga tccatcccat tcgtcggttc
    49141 atccaaaatc aaaaattctg gattcgtgat cagtgcattt gcaatagcta aacgctggcg
    49201 catacctaaa gagaaattct ttgctttctt tttccctgta ttttccaatc gcatcatttt
    49261 cagcaggtct tcgatttctc tatccgacac accgccgttc gctgcttgga tacgtaggtt
    49321 atctcttgca gttaattctg gataaagagc aggggtttca atcgattgac ccatacgtcg
    49381 tcttgctgcc tgcaaatccg tcgttccaaa taattcgatc gaaccttcat caatcgagat
    49441 caaacccata atagttctca taaaggtaga cttacccgct ccattctccc caatcaaacc
    49501 atagatcatt ccacgtttga tttcgatcga tactttatct aatgctttgg cagcaccgta
    49561 ttttttggtt ataccagttg ctttcaagac cgtttcattt ccaaaatcca tacttatcct
    49621 tctttcttat tgataagtga agtatagttg acgggtataa agaaagcagc cagaaatgta
    49681 taaagaaagt gtaaattttc caattgaatt tcaagaaaaa tcagactgtc tcgcagacgt
    49741 ggttgagttg tctgccaatc aaagagcgca ccaaacagtg ctaatccaag tggactggcc
    49801 accatggtaa tcgttgttat cgttgacatc agacgtcctt gtatatgcgt tggaacttct
    49861 cgctgaagcc agaccatata aaaagcaaaa gaaaacatta atccttaaaa acggacttat
    49921 gaaaaataat acatgccgtt tgggatttac aattagctac caaataagag gttatgacaa
    49981 aagacttttc acaatccctt cttccgaata aacggtgttc tcctgccaac tcctcggtca
    50041 caagtcacaa tatttatcaa acatgagaag caatttttgt aaattccttc ttatttcttg
    50101 aagctgacca cttctgtcac aacctcagaa gcggataaat aatattgttt gatgatactt
    50161 tatctttttc ccgtttgttt atagatcgaa tgatattata gaccaagaat cctacctatt
    50221 aacatagcta aaaacacaaa aaaggcactt tccaaattgt ggaaagtgcc agaaaagtta
    50281 ggattaaggt aactgcgctc agcattgctt cgcccagtac gattctcgta actcgtgaac
    50341 tcgaacgata atcgcaatta acgttttgag aactgtgaag ctttacgggc tttcttaaga
    50401 cctggttaaa caaaactcta ttttattgca ttttacagga atgtatttca tgcgaaatta
    50461 ataagtcttt gtttatagag ttttactagg gtataaaaag ttgtatgtta tcattacctc
    50521 aaaacattac ctcagcttgg cgagttggtt gattgaagaa taaatcattt atctagatat
    50581 ctcaataaaa aatgatttct aagaaacttg tattttcgtg ttattatttt tttgccctat
    50641 caaaatttct aattcttcta gcgtaatatg attatgacac ataatcgatt caattacttc
    50701 aatacctatt tttttcgaga aagatccctt ttgttcactc acaaaaatac tttcagaaga
    50761 catatataag gaacgaagtt ttggaattag tatatcgtac aaatagacca ccataaaatc
    50821 gtccttgcaa ccattctcaa aaagaattaa atttgctgat tgtgttacat atatcactga
    50881 attacattca tggtcaattc caaaaacaat gtctacttca ttttcttttc taattcgcgc
    50941 agttacattt ttcaaacttt caccattact tataaatgct ttagttactg taagctctga
    51001 tgtttgttca gatattcctt ttataatatt ttgttcatca gccacattcc ccataaatcc
    51061 gccgattcct tttaagtaat aattctcacc taagagtcgc ttgcattcac tgaattgata
    51121 aaaaaaccag ttaaaaaaat cttctggaaa aataaattct gatcgaactt tatttatatt
    51181 tttagctcct attaatttta taactcgatt aatgttcact tgctgcgatg aacatataac
    51241 tgaataaatt gagttgttta attcaaaaaa acaactttaa catatgcatc attaattcgt
    51301 tattttttag gtagatattg atcattgtta tctctctttt tttgcttccc atactccaac
    51361 ttagaaattg ccgttataaa atttatttta aaggtgactt tttcccctga agtatcggta
    51421 gtaggaatac ttaatacctc agcttctgag gaggtatccc aattatagtt atactttgca
    51481 tcatcaccat aaagaacaat tccatttttt ttatcttgtg ccgttagctt tactggttcg
    51541 cttacttttc ttgttaatat cttggacttt acatcatcga tattgcttat tttatcaagc
    51601 ttaaataatt tacaaattaa tttcgtacta gtttttatat ttga
//
LOCUS       DAIGOK010000003        51078 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.003, whole genome shotgun sequence.
ACCESSION   DAIGOK010000003 DAIGOK010000000
VERSION     DAIGOK010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.003"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            115..960
                     /locus_tag="NOA01_000102"
     CDS             115..960
                     /locus_tag="NOA01_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCI0631342.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1241..2461
                     /locus_tag="NOA01_000103"
     CDS             1241..2461
                     /locus_tag="NOA01_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HCI0631343.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2458..5178
                     /locus_tag="NOA01_000104"
     CDS             2458..5178
                     /locus_tag="NOA01_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCI0631344.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5162..6106
                     /locus_tag="NOA01_000105"
     CDS             5162..6106
                     /locus_tag="NOA01_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCI0631345.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6175..7185)
                     /locus_tag="NOA01_000106"
     CDS             complement(6175..7185)
                     /locus_tag="NOA01_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCI0631346.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7365..7709)
                     /locus_tag="NOA01_000107"
     CDS             complement(7365..7709)
                     /locus_tag="NOA01_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCI0631347.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7702..8136)
                     /locus_tag="NOA01_000108"
     CDS             complement(7702..8136)
                     /locus_tag="NOA01_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HCI0631348.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8274..9011
                     /locus_tag="NOA01_000109"
     CDS             8274..9011
                     /locus_tag="NOA01_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0631349.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9004..10215
                     /locus_tag="NOA01_000110"
     CDS             9004..10215
                     /locus_tag="NOA01_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI0631350.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10419..11201
                     /locus_tag="NOA01_000111"
     CDS             10419..11201
                     /locus_tag="NOA01_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HCI0631351.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11271..11933
                     /gene="trmB"
                     /locus_tag="NOA01_000112"
     CDS             11271..11933
                     /gene="trmB"
                     /locus_tag="NOA01_000112"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCI0631352.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12031..13785)
                     /gene="ade"
                     /locus_tag="NOA01_000113"
     CDS             complement(12031..13785)
                     /gene="ade"
                     /locus_tag="NOA01_000113"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCI0631353.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            13917..14846
                     /gene="tadA"
                     /locus_tag="NOA01_000114"
     CDS             13917..14846
                     /gene="tadA"
                     /locus_tag="NOA01_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HCI0631354.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            14803..15852
                     /locus_tag="NOA01_000115"
     CDS             14803..15852
                     /locus_tag="NOA01_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HCI0631355.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            15849..16127
                     /locus_tag="NOA01_000116"
     CDS             15849..16127
                     /locus_tag="NOA01_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCI0631356.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16102..16572
                     /locus_tag="NOA01_000117"
     CDS             16102..16572
                     /locus_tag="NOA01_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HCI0631357.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16589..16891
                     /locus_tag="NOA01_000118"
     CDS             16589..16891
                     /locus_tag="NOA01_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631358.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            16908..17279
                     /locus_tag="NOA01_000119"
     CDS             16908..17279
                     /locus_tag="NOA01_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HCI0631359.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17251..17610
                     /locus_tag="NOA01_000120"
     CDS             17251..17610
                     /locus_tag="NOA01_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631360.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17782..18789
                     /locus_tag="NOA01_000121"
     CDS             17782..18789
                     /locus_tag="NOA01_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCI0631361.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            18814..19998
                     /locus_tag="NOA01_000122"
     CDS             18814..19998
                     /locus_tag="NOA01_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCI0631362.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20435..22018)
                     /locus_tag="NOA01_000123"
     CDS             complement(20435..22018)
                     /locus_tag="NOA01_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCI0631363.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22596..22739)
                     /locus_tag="NOA01_000124"
     CDS             complement(22596..22739)
                     /locus_tag="NOA01_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCI0631364.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(22962..23441)
                     /locus_tag="NOA01_000125"
     CDS             complement(22962..23441)
                     /locus_tag="NOA01_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCI0631365.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23514..23798)
                     /locus_tag="NOA01_000126"
     CDS             complement(23514..23798)
                     /locus_tag="NOA01_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631366.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(23876..24070)
                     /gene="ssrS"
                     /locus_tag="NOA01_000127"
     ncRNA           complement(23876..24070)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="NOA01_000127"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24142..25428)
                     /locus_tag="NOA01_000128"
     CDS             complement(24142..25428)
                     /locus_tag="NOA01_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HCI0631367.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25566..26237
                     /locus_tag="NOA01_000129"
     CDS             25566..26237
                     /locus_tag="NOA01_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HCI0631368.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26203..26691
                     /locus_tag="NOA01_000130"
     CDS             26203..26691
                     /locus_tag="NOA01_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HCI0631369.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26704..27651
                     /gene="prmA"
                     /locus_tag="NOA01_000131"
     CDS             26704..27651
                     /gene="prmA"
                     /locus_tag="NOA01_000131"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCI0631370.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27652..28416
                     /locus_tag="NOA01_000132"
     CDS             27652..28416
                     /locus_tag="NOA01_000132"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCI0631371.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28649..30862
                     /locus_tag="NOA01_000133"
     CDS             28649..30862
                     /locus_tag="NOA01_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HCI0631372.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            30873..31319
                     /locus_tag="NOA01_000134"
     CDS             30873..31319
                     /locus_tag="NOA01_000134"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCI0631373.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31490..32749
                     /locus_tag="NOA01_000135"
     CDS             31490..32749
                     /locus_tag="NOA01_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HCI0631374.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(32838..33785)
                     /locus_tag="NOA01_000136"
     CDS             complement(32838..33785)
                     /locus_tag="NOA01_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCI0631375.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34179..35471
                     /locus_tag="NOA01_000137"
     CDS             34179..35471
                     /locus_tag="NOA01_000137"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCI0631376.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35486..37255
                     /gene="aspS"
                     /locus_tag="NOA01_000138"
     CDS             35486..37255
                     /gene="aspS"
                     /locus_tag="NOA01_000138"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCI0631377.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37364..38239
                     /locus_tag="NOA01_000139"
     CDS             37364..38239
                     /locus_tag="NOA01_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCI0631378.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38505..40388
                     /locus_tag="NOA01_000140"
     CDS             38505..40388
                     /locus_tag="NOA01_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCI0631379.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40463..40747)
                     /locus_tag="NOA01_000141"
     CDS             complement(40463..40747)
                     /locus_tag="NOA01_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCI0631380.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40737..41471)
                     /locus_tag="NOA01_000142"
     CDS             complement(40737..41471)
                     /locus_tag="NOA01_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HCI0631381.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41600..42223
                     /locus_tag="NOA01_000143"
     CDS             41600..42223
                     /locus_tag="NOA01_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HCI0631382.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42415..43536
                     /locus_tag="NOA01_000144"
     CDS             42415..43536
                     /locus_tag="NOA01_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCI0631383.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43543..46671
                     /locus_tag="NOA01_000145"
     CDS             43543..46671
                     /locus_tag="NOA01_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HCI0631384.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46690..49884
                     /locus_tag="NOA01_000146"
     CDS             46690..49884
                     /locus_tag="NOA01_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HCI0631385.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50030..50737)
                     /locus_tag="NOA01_000147"
     CDS             complement(50030..50737)
                     /locus_tag="NOA01_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCI0631386.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16714 a         8933 c        10613 g        14818 t
ORIGIN      
        1 tcctattctt aaaatgatgt ttgtacttac ttaaattagg tataattagt atatgttttg
       61 tgagaaattt agacttacaa taagcagtga atttataatc aacgtatagg agacatggat
      121 gaaatgaaac taattggtat agatttagac ggtactcttt tgaattctga ccagagcatt
      181 agtcaaaaaa atgcaagaac aatgaaacat ttaccggaag actgttttcc attcatttgt
      241 tctggaagag aagtagaaga cattaataat attctcaaaa aatctggttt aaggatacca
      301 gcagtagggt taaatggttc tgttggattt gatggagaaa aaaagatatt tgagtcttcc
      361 ttcgataaaa atgatgtaaa aaaaattgat tctgtcctat cgaaatttcc aaccaaaata
      421 tatacaaatc tcggaagtta tgaatctcag aattataaaa ataaaatgaa aaaaattttt
      481 cacgaggtag ggagtgaatt ttctatcaaa gagctaaact atgagcttga gtatgaaaaa
      541 tctatttgtt caatccctta taataatatt gaggaaatta tagaaaagga tgctatcagc
      601 atttacaaat tttttatttt tattcctaat cgtcaattga aagctaatat atttaaccat
      661 ttgaaaaaga tgctaaatgt gtctgttact gaatcggcac ctgtaaatat tgaaatcgta
      721 cctcaaaatg tatctaaagg tgtggtgttt gatcgtttgg aatcaatcta caatttaaag
      781 aaacctttga gaatcgcgat cggagatagt ttaaatgact tatctatgtt tgaaagtgca
      841 gatattagtt ttgcaatggg aaatagtcat caagaaatca tgaaaaaagc tgattatgtc
      901 actgtttcaa atgataaaga cggagtatct caagcatttg aaagaattct taccatataa
      961 actaattaag gtagtataga acatagaatt ctaaatacgg aaaaatggag cataaattcc
     1021 agtacaaata gtacttcttc agtgtcaaac gaagcgaata taacatagat atttaggatt
     1081 gtgacaaaaa tcatgtcaca atcttttttt cgaataaact tattttttga agtcggccat
     1141 ttatcgtaat ttctttgcat aatacgaaca aattttcggt tattgatgaa aaaaaacatt
     1201 gaaaatggta tgataaaaga aaaaaaggag gaaattttta atggtgaaat ttatccatgc
     1261 ggcagattta catatggatc ggtcatttga aggactgaca actcttgata aaactgtaca
     1321 ggaaaaacta ttgaagacga atctgaccgt tttatcaaat atcattgaac aggcaataat
     1381 aaataacgtt gattttgttt tacttgcagg agataatttt catcaaaatc gtccttcatt
     1441 aaaaatacag aaacattttt cagaacagat gaaacggtta gaaaagaacc atatacctgt
     1501 atttataata ttcggaaatc atgatttcta tcaaaaagag cggtattggt tctcctttcc
     1561 taaaaatgtc catttattta catccgaaac ggtagagaca aaaaaaataa cgataaaaag
     1621 tggagaaact gtctctctca gtggatttag ttatcgtcaa ccgtggattc aaaaagacaa
     1681 agtgatggaa tttccttcta gagaactcac agattaccat attgggttgt accacgggga
     1741 acctggcgtc tctcaaaaag gaaactatgc tcctttccaa ccctcaaaga tgcaagaaaa
     1801 gggatacgat tattgggctt tagggcatat ccatgtgcca acggtcttaa atgaaaagca
     1861 aacaattgtt tatccaggtg cgcctcaagg tcatactaaa aaggaacaag cttctacatc
     1921 tattctacta gttgaattaa gtaaaggaag ttgccaaata catccgatta aagtagcaga
     1981 ggtctattgg aagacaaagg aaatatcatt gaggaaagca agaacgacaa aagaggtcat
     2041 tgttcacatt cgtcagcagc tttcaaaagt agaggatgga tttagtttaa ttgagataaa
     2101 attaaaagat tatgaccatc tcaatacaga tgttttggaa cggatacaat caggagagct
     2161 attagactat ttgttagagg aattctcaga taggataaat gatttcttta tttggcgtat
     2221 ttctctcata gagaatactt tttatacgaa gaccccactt gctgcttctg ctaaattgat
     2281 ggaacagtta tttcaatact atcagacacc tcaagatttc caacaaattt taaatgaagt
     2341 atatagtcat caagaagcgg ctagaatatt gagcgaactg ccggaatatc aagaagaaac
     2401 acttgaaaaa gccaagcagc tgcttaatca agatttttta tttgaggagg atcaggaatg
     2461 aagattttaa ggatcgagat tgctgctttt ggaaaatggc gtcaaaaaag ttttgacttt
     2521 tattcaggca atcagctgat ctatggggga aacgaagcag gaaaatcaac gatttatcaa
     2581 tttattcaag caatattatt tggtttccct tcaaaaggcc gtaaaaaaaa ggattataca
     2641 ccaaaagacg gctcagccta tggcggcaaa atatggctta aacatcctgt atatggtgaa
     2701 tttgctgtcg agcgctacaa gcagcaaaat cgcggaaaat cgaaggtgtg gctgggggat
     2761 caagtgggaa gtgatgagtt actagaaaaa ctgatcgcac ctttgacgaa agagttattc
     2821 cagcaggtat ttactttcca gcaagagcag ttgatggaac tagatcattt gagagaaaaa
     2881 gagctacatg atgcgctgat ttctttaggc atcacgggca gttctcaagt attccaaaag
     2941 cggcaagaat attatcagaa agcacaacaa ttattcaaga aaaaaggcca aaaattacta
     3001 atcaatcaaa aattaaatga atggaaagag ctgcaagaaa aaattcatca aaaacaagaa
     3061 caggaagctt attttggcaa gttgatccaa caagaacagg aatacgagtc gcaacagcag
     3121 cagttattgg cacaattaaa agaggcgaac aaacaatttt cattagccag acagcaagaa
     3181 cttaattttt ctttatatga agaatggcaa tctctcaaaa aaaacagcac tacaggtctt
     3241 cctgatgagc aacttctttt agatctggag tctttctcca aacggtatca gcaaacaaat
     3301 gagcgtttgg aagaattaga agcaacactg gaaaagcata gcgggatgga tcagcagtca
     3361 gccagatatt atttttattt agagcaggag caggaactaa aagatctcca gcaacttcaa
     3421 tttacagcgg aacaattaaa cgaagaatgg caaagattac aaattgagac tagagaaaca
     3481 aattcagaat tgaagatttt ggaacaaagg tggcattggc agaaaaatcc gttaccggaa
     3541 gaatttttat atgaagaaga atggaagcat cttctggctg aagaaagaag gattgctgat
     3601 ctagaaagcc agatttctgt gcgtctccaa atagctaaag aacagcaaga gatgattgaa
     3661 gcagaagtga atgacttgga agagacctat ccagcattat tacaaaatca gtcagagata
     3721 gcaccagtct cttcatctaa accgtatatg ctgatggctg gcggagcaat aatcattgca
     3781 ggtatatttg taccaatgcc cttgaaagtg atccttttac taattggtgc aggcatgatt
     3841 ggctatttct tctacaaaag gcaaccaaaa attaaaacta aaaatgattt ctctgagata
     3901 aaaggaatgt ggcaagaaaa actaggacaa cttgatctag ccttagctca attagctgaa
     3961 gcagaagaaa aacaaaagag aactggagaa gaaaaacaga ttcttcagca agaagtatat
     4021 cgttttgcgg atcatcggca cttaggaaag atggatacgc tcaaactcat gaaagaatat
     4081 ggacaggaag tcatagatta tcagaaatta tatggcgtat ttgagcggaa acataaaaga
     4141 gaacaggaaa tacaagaaaa actacgacaa atcgaccaaa aatttgagtt tgttaaagaa
     4201 tggttaccga tacaagataa gacaatttca gaaaaaatgg tattgctttc ccgttttata
     4261 caagagatgg aacagatgaa atttgccaga agctatcagc aaaatacttt attgaagcaa
     4321 gaaatcaatc aattgaaaaa gaaacaaaaa gtatggatac aagaatacag cgaacaattg
     4381 actcaagcag gcttatctta tccttcggaa gtacctgtct ttttacaaaa agccaaacaa
     4441 gccgaacagc agatgaaaag atacaatgaa ttatcggaaa tgcttactcc actatttcct
     4501 gaagagctga caaaagagaa attatctgta agactgcgcc aactgaaaga aaagcaatcc
     4561 caactccagc aagaagaaaa agaagcaagt gaaaatcggc aacgactcca acttcaagta
     4621 gaagagttgc aaagagacgg cacattggac gaactttatc aacaagctag ccggcaaaaa
     4681 gcaaaattac aggaattact ggaggaatgg ggagcacttg aaatcgccgg gacattctta
     4741 ggtgatcttt caacagagat gagtgaaaga caattacccc agttgttaag agcagcaggt
     4801 gattatttag gtattttaac aaaaggaaat tatcagaaga tattactgaa aaatgataca
     4861 ctgactttaa cagacggata tacagaattt cccatctatg agttatctac aggtacgaaa
     4921 gatcagctga ttatggcgat gagatttgct tacctctcat tagaagctaa ccgttcgata
     4981 tgcccgatca tcattgatga tggctggctt cattatgatc atcagcgaaa gtatcaatta
     5041 gctgaacttt tagctaaatt tggagaaaag tatcaaataa tttgtttttc ttctgataaa
     5101 gaaatggtaa gctattacca tgagatgaac caaatcgtaa aacaattaga aggagcaaat
     5161 aatgaaaaaa attcgtgatt tagtaatcga tgagcagttt gaacaatttg tcctgatcaa
     5221 aagtgcagat gtaagaatcg ctaaaaacgg aaaaaaattt attgctttta ctttccaaga
     5281 tacttcaggt actatcgatg gtaaatactg ggatgcttcg gaggaggaaa tcaataaatt
     5341 tacagccggc acagtcgttt ttctaaacgg taaaagagag gtttatcaag gaaaccctca
     5401 agtaaagatt cttcatatgc gcttaacaag ggtagatgag ccaggagagc ctagtttata
     5461 catggaaaga gcccctttga agaaagaaga aatggaagaa gaaatcaatc aattgttgtt
     5521 tgagatcacc aatgcgcatt ggaatcgtat tgttcgctat ctacttaata aatatcaaaa
     5581 acaatttttt gagtttccgg cagcaaaaag aaaccatcat gcatttgcca gtggattagc
     5641 ttatcatact ttgacaatgc ttcacttagg gaaatcaatc gccgatgaat atcctgagct
     5701 gaatcgagca ttgttgtatg ctggtatcat tttgcatgat ttaggaaagg tgattgaact
     5761 ttccggtcct atgtcgacag aatatacgct tgcaggaaat ttgatcggtc atctggtatt
     5821 gattgacgaa gaaataacta aagcctgtct tgcgttgaaa atcagcgaag ctgacgaaga
     5881 tgttctcgta ttacgtcata tggttttagc acaccatggc ttattagaat atggctcacc
     5941 tgtacgtcca agaataatgg aagcagaaat attacatcaa atcgacaata tcgatgcttc
     6001 gatgcagatg atgttgactt cgattcgtca aacagaacca ggaaaataca ccgatcggat
     6061 tttcggtatg gacaaccgaa gtttctatgt accaaaagat ttatagcaaa aatttatttt
     6121 tttcattcaa aacaaaaaaa gcgagacata agtcttcgct ttttttgttt tcttttattt
     6181 tgatgaatca gttgtttctt cagttgaaga ttcttttgtt gaatcagttg attttgtgtc
     6241 agaactcttt gttgaagctg tagtttcact tgagtctttc gtacttgatg aagttgttgt
     6301 gaatgcactc aacacatttt caaaggcatc atctttgatt tttacatttg catcttttaa
     6361 ttcttcaccg attactttag ttgtgaatgc attgtcactt agtttagttt cagtagcgat
     6421 atcttttagt tgatctttgt atttatccat gtcattgcct ttattttggt ttttcaccat
     6481 tttgacaacg tagtattctg tcgcataaga agtaggattt gttgtagtaa taacatcgga
     6541 gatctcaccg tctttcaatt tgaaagctgc ttcttttact tctgcaggaa tagttgttgt
     6601 cgttgagtcg aatttaactt taccgccatc ttcttttgtc tctgtatcag ttgatttgtc
     6661 tttggctaat ttagagaaat catctccatc atccgctgat tttttcacat cttttgcttc
     6721 atcttcactt gataatttga tgatttgtgc ttctacttct ggatggaaag atttccaagc
     6781 agttttcaaa tcgtcatctg taatatctac gtgagctttc aagccagctt caaaagctaa
     6841 attgttacga atatattctt tataagtatc tttcgtatat ccagcagctt ctaattgtga
     6901 ttcaaaagta tcacctaatg atttcgcttg tttgtcgtat tctgcatcca cttgcttatc
     6961 ggttacttta tcgccatatt tgttattgaa tactttataa atgatcatcc gttgtactaa
     7021 ggattggttc gatgattcaa gttttgcttc atcatagaaa tcagatacag tgatcgttcc
     7081 gcctttcatc gttgcgatat ctttattagt atcgcctgaa caagctgcta gagttaaaac
     7141 agccaacgca ctagtagctg ccaaaataat tgattttttt ttcattaaaa tttgcactcc
     7201 tgtaattagt tattattttt atatattaac tataccatat cccatttcct ttaaaaacgt
     7261 tgtcgtggta gtttcaaaca aaattcaaaa tttcatcaca ttcctaggaa atacgcaaac
     7321 ttttctcact tatatagtta aatctgttga gaaaataatc attattatat ttgttcagga
     7381 tactctggca gatcagcttt tagtttttct acttgttcat tgatttgtgt gatacgtggt
     7441 tcggcttgga atttaaaatc ttcgatatct tttttgattg cttcttggaa agcaggaatc
     7501 acagattcag ctgtttcttt tgtttcgatc aatgcgcttt taaaatgatt caggctgtta
     7561 tttaaatcat ttgtcagagt tgttgcttct tccaattctt cgatcagttt ctttcttgtt
     7621 tcattcccgc tcctgggcgc caataacaga cctccgacag tgccagcagc agctccaaat
     7681 aacagtcctt tgaaaaagtt tttaatcatc gcaggacacc tgctttacga ttgtttcttg
     7741 aatagctttt agttcttccg atccataact atcttgatga ttgacaaagt gaatagaaaa
     7801 atcatcctct ttgctgtagc gagggactaa atggatatgt gaatgaaaaa cagattgata
     7861 ggctagttca cggttgttat tcaagatatt aagacctttc atttctggaa aagctttttc
     7921 caatgcttga gcaatcttag gcacacgcga aaaaacctct cctgcaagtt cgggatcata
     7981 ttcaaaaata tctgctacat gacgtttagg cacaactaat gtgtgtcctt tcgttgcttg
     8041 agagatgtct aaaaaagcat agaccttgtc gtcttcatat attttatagc ttgggatttc
     8101 ttgattgatg attttgcaaa aaatacagtt ttccatataa aacatccttt ctataaagaa
     8161 taaatacttc agcttaactt taccataagt taaagctatt gtaaggaaaa aaacaaaata
     8221 ccgtcatttt cttcgtggta taataaataa aaataaatgg cggaggaaaa agaatgagtt
     8281 taattatcga tcatgtcaca ggggggtacg gacatctccc tgtgttaaaa gatatcagtt
     8341 tcgatgtaca gcctggagag atagtagggt tgattggact gaacggagca gggaaaagta
     8401 cgaccatcaa gcacattatc ggtctgctgg aagcttcaaa aggaacgata gaaatcaatc
     8461 atctatcctt aaagaaaaat acggaagaat atcgcaaaca aattgggtat attccagaga
     8521 ctccttcttt atatgaagaa ttgacattga gagaacatat cgaagtcact gcgatggcct
     8581 acgatatacc tatggatata gcaatgaaaa gagcagaaaa gttgttggag acttttcggt
     8641 tatctaatcg tcttgactgg ttcccagcta atttttcaaa gggaatgaaa cagaaagtga
     8701 tgatcttatg cgcctttttg atcgagcctt ctttatatat catcgacgaa ccatttttag
     8761 gcttggatcc actagctatc aatgcattgc ttgaattgat ggtagaaatg aaaaaagaag
     8821 gagccggtat tttgatgtct acgcatattt tagctactgc agaaaaatat tgtgatcgtt
     8881 ttgtggtctt acataacgga gagatacgag cagacggttc tcttgagatg ttgagaaaag
     8941 aatttcagct gccggattct tctttggacg aaatctatat tgctttaaca aaggagcagg
     9001 cacatgagta gtttttttca aaaacgttta gcaagacatc aaaagaaaat gatgaaatat
     9061 atgcgttatg tattgaacga tcattttgtt ttagtttgtc ttttcttgat tggcggtgtg
     9121 ggattatatt attctaattg gttgaaaacc ctgcagccac cgtttgcaat tggagggctc
     9181 ataatctctg tattttggat gctctgttta ttcgtgggta agatggctac ttttgcagag
     9241 gcagcagata ttgttttttt acttcctaaa gaaaaagaaa tgcaaaccta tttggaacga
     9301 gcttttcgct attcttgcat ttttccgttg atctttttga tattggcatg cggattggcc
     9361 atgccattag tggtggttag tacggagcaa tccttttcga tgtttttcgt ctatctgatc
     9421 atattatgga gtttgaaatt cagtcatctg caaatcaaga gagcagaatt gtttcgacta
     9481 gatcaacaag ttattcgtta ttggaaatta ggctggtttt tttcttctct gatctttttg
     9541 tttctatcat tttattggct aatatgggca ggaatagcag gtgcattagt acaggcaagt
     9601 ctatattact ggtttttgtg gaagcaacaa gaaaaaggat tggattggga gaaaatggtg
     9661 catgtagaag aacataggtt gcatcagatc tatcaattta tcaatttatt cacagatgtt
     9721 cctgaaatta cggcaaagat caaaagaaga cgctatttgg atcctatatt gactcatatt
     9781 aaaagagaat caaaaaacac ttatctttac ttgtttgcaa gaagattggc aagaggttct
     9841 gattttagtg gtctttattt ccgattagta attataggtg ggttattgat tgccggagta
     9901 gtcgactttt attttagtct gattatcggt ttcttatttt tatatttgat cggtttccaa
     9961 ctgttacctt tagcgaacca gttccgctat atggttctcg ttcagcttta tccaatccat
    10021 aagaatcaga agaagcaggc gattcagtat ctactcacat gggtattaat tatcgcgtcg
    10081 gttatttttg gtttgattgc tgcagtcgta ttagatgggc agcagcgatg ggttccgcta
    10141 gcggcttatt tgattgttac tatgctcttt gttaaactat atgtaccaac tcgtttgaag
    10201 aagatgtcag actgaaaaca aagctgtgta gggtaaaaat gagaatgaaa aatagagcgt
    10261 tgacagtaag aatgctgttc gttctatttt ttttgagaaa agctggtttt ttgaatggca
    10321 attaaatgaa aaagaacgta tatgtgcttg acgtgtagtg ttcaggcgac tagaataata
    10381 agtactgcaa aacaaagagt aagaggacgg gagaatgcat gatggagttt cagttagacc
    10441 aagagtggcg gttacgtccc atcaaaggcg acacaggaaa aacatacata ggcatgcgtg
    10501 atgaggacaa agtctttatt aaacgaaaca cgactccgat gttggctgct ctttcaaggg
    10561 aaggaattac accaaaactt gtttggacaa aacgaacggg gaatggcgat actttgacag
    10621 cacaagagtg gctggaaggt cgcttgctga ctcctaatga gattggtcaa agaaacgacg
    10681 tgattgatgt tttgtaccat ttacatcatt caaattctct taaaaccatg ctgacccgta
    10741 tcggtggtcg tgtcatgacg ccggagaaga tgctgcaaaa atatgaaaaa gaattaccgg
    10801 aagaactcca gaaaaatcaa tttctgcaaa ttgtccatcg ttttcttcgt gagcagatcc
    10861 cgtcaataga taaggaaaac ttatcagtag tacacggaga tgtaaaccat cgtaattggt
    10921 tagtatgtaa aaattattta tatctagtcg attgggattc aatcatgttt gcagaccctg
    10981 cggtggatat tgcaacgatt ttaggaaatt atgtgccact gtctagctgg aatcagtggc
    11041 tgatcagtta tggcatacgc ccgactgacg aaatgcttga aaaatttcat tggtatgcgg
    11101 ccatgaattt gctccaagaa atcaccagat attgcaaacg tgaagatcat cgacggatga
    11161 acgaagaaat tttacaatta aaacgaattt tcagtggctg aggtttggcc caaactctgc
    11221 ggagatttgg ccagaccttt ttaattagag gaaaggaatg gagataaata atgcgtgtcc
    11281 gaaatcgtcc gggagcagcg gaaatgttag cggctcaccc gaattttgta attagtgatc
    11341 caacattgtg gaagggaaaa tggaatgaat tgtttgaaaa cgatcatcca atccacattg
    11401 agattggtat ggggaaaggc cagttcatca ctggaatggc aaaagcccat cctgaaatca
    11461 attatattgg agtagaaatg caggtcagtg ttgtttcgat tgcactagac aagttgatcg
    11521 aacaaccgct tcctaacctg aaacttctac atgtggatgg atcagcgttg acagagtatt
    11581 tcgctgacag cgaagtagat caaatctatc tgaatttttc cgatccatgg ccaaaaaaac
    11641 gtcatgaaaa acggcgattg acatataaga cttttcttgc tgtagatgaa cagattcttc
    11701 gtccaaatgg agaaatccat ttcaaaacag ataatcaagg attgtttgag tattctctgg
    11761 caagtttttc tcaatatggc atgatcctca aacaggtatg gttagattta catcaaagtc
    11821 aatttgaagg caatattatg acggagtatg aagaaaaatt ttcttcaaaa ggtcaacgta
    11881 tttatcgagt agaagctcaa tttcaagata agaccaaaaa gaaaagtctg tgaaactaac
    11941 tttctgcttt cataaaaaca ttaatatcca taaataaatc ccagaaaatg aacattacag
    12001 atgtaatgta ttttctggga tttatttgat ttaaaatgta tttatttcat cggtttttcc
    12061 acaaggtgga atcaattgga agcgagaaaa tgaaaataat cctttgtcgg tgatcttcaa
    12121 ttcgggtatg acagataatg ttaagaaaga aagtgtcaaa aaaggatcaa aggttttagc
    12181 tgctcctagt tgatgtgctt tagttattaa ttcattcaac tgttcataga cgatcgaaaa
    12241 aggttcctgt gtcatgattc cgcctatagg aagagaaaca caagctattt cctgctgttt
    12301 gttgacaacg gtcatcccgc cacctttttg aatcaaaaga ttagcagcat acagcatatc
    12361 ctcgtcgttt gtcccaacga caactagatt atgcgagtca tgggcaatcg ttgtcgctat
    12421 cgctccttgt ttcagctgaa atcctttcac tatgcctaat ccaatgtttc ccgtttgatg
    12481 gtgacgctcg atcaccgcaa tctttaattg gtctttttcg attgaaggaa tgaatcgttc
    12541 gccttgtcgg tccacttttt ctaccaaatc tttagtcact agactattcg ggataatttc
    12601 gatcacatgg gcttgtgaag aagaaagaag cagctctaac gaatgtggat ttaatggttg
    12661 agcgtttagt tttggcagac aacacgtata tgaatttgaa tgatcagcaa ataaatggtc
    12721 ttggagttgc ccagattgaa caactaatgt tcccttatag aaaacttcat gaattggcag
    12781 cttggtaaga tcgtctgtta agaagaaatc agcttgatac ccaggggcaa tcgcacctag
    12841 tgttttcaaa tcaaaacatt ctgctgcatt caatgttgcc atctgaatgg cttgtaatgg
    12901 atcgattcca agtgcaatag cttgacggac gatatgatcg attgatcctt cttttactaa
    12961 atcattgatc agtttatcat cggtgacaaa gaagcatctt cttgaatttt caggtgtgat
    13021 cgccggtagt agcgatgcca gatcttttgc aacggtccct tctcgaacca ttaaatacat
    13081 accaagatcc aaacgttctt tagcttcttt tgcagttgta gcttcatgat ccgtccgaat
    13141 acctgctgca ggataaacat ttaactcttc catgccgata ccagctgcat gcccgtcaat
    13201 ttttttctta tgctggtgca tcaattggat cttttctagg atgtctgttt cattatttgc
    13261 tactgcttga tagttcatga cttcagctaa gcctaaaact tcgggttttt gtaaaaaagg
    13321 ggctaatttt tttgcattta aatttgcacc attggtctca aaaggggtta caggtacaca
    13381 agaaggtagc ataaaaaaga tattcatgag tgtttgtctt gcatcttcta tcataaactc
    13441 aagacctgta ctcccagcaa tgtttcctat ttcatgggga tctgtcacta cagtagtaac
    13501 tccatgaagc aaggatactt ttgaaaattc gcttggtgat aaaagtgagc tttcgatatg
    13561 cacatggcca tcgatgaatc ctggcactag atattgccca ttagcattat gtacgctttt
    13621 tccttcgtat tcaccgattc ctacgatcat tccttcgcag attgctacat ctttttccat
    13681 gatttctttc gtaaagacgt tgacgatctt agcatgcttg atcactaggt cagcaggctt
    13741 ttttttactt gctacttcga tatatttcct taatgttgtt tccaatggag tgcctcccaa
    13801 gataaatttt tataagtata acataaattc ggtttttgcc aatatttttt tgtaaattca
    13861 ttttatttga aacaaaaaga tcaaaaagac gtatgttttt tgtggagggg agaataatgg
    13921 aaataaaacg attatcggat caaatgataa gtgaaagttt tgccaaacat gcccaagacg
    13981 tatatatatt gccagcttca gaagtatttt acatctattt tcgctatgga aaaaaacgaa
    14041 cattctggaa gaaagtctct tcggcagatg gacagcaact gatctcaaga tttaagtatt
    14101 tgggacagat ggatatcgga gaaaaaagaa aagctcagtt aggtgcattt acttattctt
    14161 tagaaaaaca ggaagttcgt ctgcggttgt cttctgtagg agattacctt caacgagaga
    14221 gtttagtgat tcggctatta tacggtactt ttgaaacacc ccatcgttgc tttagttctc
    14281 gagattttgc aactatttta cagaaaaccg tcagacgtgg attatacctt ttcagcggac
    14341 cagtcggatc aggcaagact tctctaatgt atcggttggc tttggaagaa aaattgcaag
    14401 tgattactat cgaagatcct gtagagatag aagaaccgtt ttttctgcag ctacagatca
    14461 atccgaaaat agcacagact tatgatacac ttttgagact cgcattacgg catcgtccag
    14521 acttgctaat cattggcgag ataagagatg aatcaactgc acatgctgcg gtaagagcag
    14581 cactaacagg gcaccgggtt tttgcaaccg tacatgcacg caatcttgaa ggtacattgg
    14641 tcagaatcca tgaactgaca aagaaaaaag aagagctatc tgaatgttta gctggtgtca
    14701 tttatcaaga acttttggaa gatgccaatc accatccttc tgttttatgg agttatcaat
    14761 ttatggagga agaatcagta aaaaaatcat gggaggaaag ttatgaagaa gctcaggaaa
    14821 atatttggca gtcagaacta ttctaaagga ttaacaagaa gccagcaaaa ccagtttgca
    14881 caaaccatgg gagaactgct tttatctggt tttagtctgc aagaatccct ttatttatta
    14941 ctcacgatcc gttcgtttcc acctgttatt ttggcaaata tccaacatca gctaaaggaa
    15001 gggcaagcgt ttagtgaggt actttcgcaa ataggatatg gagcagagca actggtacag
    15061 atcgagttag ctgaacgtca tgggaatctg ccgacaactt tgttggaaat tgccaaacag
    15121 tttcggcttg ttgaaggatt tcgcaatgat ttgaaaaagc tgcttgcata tccgatgcta
    15181 ttgctcacct ttttgctagg tatcttgttt agtatgcggt tgttcgtttt gcctcagctt
    15241 attggttcag gaatggtcat gcaagagcat tggggtattc agctgataca agtctttcat
    15301 tggtatgttc tactattcat cctagcggtt actctattgg ctttactagt aaggtatcgt
    15361 ctgatgaagt tgtctcctgt tgatcagagt gaatggataa gcagacaatt attgattgga
    15421 ccgatatatt ccttgtatca atcggcttat ttgtcactag agattggaaa gctttttcat
    15481 gaaggtttcg aattaaggca aatcatagtc tgcttaaagg aaacgaaaca agaaagtctg
    15541 attcagcatc ttgcctctaa gttgattcaa ggattggagg caggtatgtc attggctgag
    15601 cagttccaga attatcgttt tctatcagaa gaattcagta aaattgtttt acagggagag
    15661 gcaaagggaa gtttaggtaa agaactgatt ttttacagcg aatggacaag aaaacagctt
    15721 ttcaaacgtc ttcatcactg gatgcatttt attcagccaa tcattttttt gggcgtagcg
    15781 gctttgattt tattagttta tgccgctgtt ttattacctg tttacggaag catagaggag
    15841 gttttgccat gaaaaagaga aaaatatctt attatagcgg ttttacattg atcgaaatgc
    15901 taatcgtctt actgatcatc agtgtattag tgttgttgtt cgtccctaat ttgtcgcgtt
    15961 ataggaatca tgtagaccaa gaaagtcgtg aagcgatcat ccagttggta gacacacaaa
    16021 aagagctcta tgcgcttcaa aataatggaa gagttcctac tgtggaggaa ttgttgaatg
    16081 aaggctatat caaacgagaa catgcagaaa tctatcagcg gccttgaagg taaggtttta
    16141 gcatcaggat atacgttgtt tgaatcactc ttactgattt tattacttac aacgatcctt
    16201 tcttttccag ttctcgcatt ttctgcatgg aagaatgaat tggcagtcta tcaatttttc
    16261 ggccaatttg aaaaacgaat ctattctacg caaaaaatcg ctattgtgaa tcagatccaa
    16321 acaggctttt attggaatag tgaagagaat caaatcattt ttcatgttcc gaatccagat
    16381 aaaataaatt gggaagtatt agatattcct gatgagatta ccttgaaacg ccatgcttct
    16441 attacttttg ctgctagaac cgggaatgaa agttcactaa aagcttatca attttattgg
    16501 gaagaaaaag agcggacgat tacgtatcaa ttccagatag ggagtggtcg ctatacaaaa
    16561 agaattgaat aaaacaggat ttgttttgtt ggagtccctt acagcgctta tgatcgcttc
    16621 tgtctgtgtc tacttagtga gctttggcca gctgtttttg cttcaggcag aaaagaaaag
    16681 tggggaggaa ctgctattta ttcgaatgat ttatgaagat atcaagacgc agcggatgta
    16741 tcacaaaaga ccagagcgaa gggaagaaat gatcccatac gatatccaaa ttgtcgataa
    16801 taatggagta atcgaaaaag tgtttgtcaa tatggagaaa gaagcgatca gtattgaaaa
    16861 aacaagtaat tccagcattt acgctgattg aatgtctaat tggactgatg atattgagca
    16921 gtctattgct cacattcaat cttttgattc agcaaaccat ccaagtaagt gaatttttgc
    16981 agcgtaaaga tcaaaaagag tggctaatat ttttagcaca attagaagaa gaactcaaga
    17041 gcagtcacaa tgtctctgtg aaaaatcagc aactgcacta cacgatcgag gataaacagt
    17101 atatcatcga aagatatcag gcaatgatac gtaaaagaag aacttccggc ggacatcaac
    17161 ctatgttaac aagtatcact gagcttcaat tattggaaaa agaacacaca atttcttttt
    17221 atgtacactt tgaaaatgga gaagatggat atgccatatg gacaaaaaac gatcagtaga
    17281 aaatgttata agggctctat tttattttct gtcttgcttt tatttctttt atgtacgttt
    17341 ttattgatgg cagcattaga aagctaccgt ttgtcaatgg atcttaatat acgaatcaaa
    17401 aattattata tagcaaaaat aatgaagcag atggctttat cggaacagtc tacgcttgcc
    17461 gaaagttcgg aaggagtttt ttactacaca actggatctg taacatatca gtgggtacaa
    17521 cagtccctat tcttagaggt ggaagtctca ccgtttatct ttcggttcac agaagaagta
    17581 aaaaatgata cagatactag tacagaatga tggaatgttc gtgaaaacgc cctcttttct
    17641 gttataacac tgtttttaat aaaaaaatgt ttgccgtttc acaaataaaa aacagtgaaa
    17701 acgattgaaa ttttgtcaat tgttcgatag aattaaacag gtgaaaaaat tgttataata
    17761 tatctagaaa tgaggtgcac catgttacca gaaaaaatgg aacaagggtt taatcaaaac
    17821 ctggaagcta ttcagctttt acagaatgca ttgggaactt cctttttaga agcctatgtc
    17881 gaaaatgcag aaaatttaat agatgattat caagtacgtg tggtggatgg agtgccgaca
    17941 aaagagacaa cgcaacggat cacagctctt tatgaagagt tgaagaaact ttctttcgag
    18001 ccagaagaat ggcgcagact gtctcaatta cttttattga agggcagcca gacagaacat
    18061 ttgcaggcga accatcaact cactcctgat agtatcggtt ttttatttgt ttttcttatt
    18121 gaacaactat acacaaataa aaaagcaccg gttaagattc tggatattgc ggcaggaatg
    18181 ggcaacttgt tattaacagt tcttttaaac ctatcgaatg cggggtacca aacagaagga
    18241 attggtgtgg atattgatga tacattgttg gctgtcgcag cttctacaag tgatttgaca
    18301 caagcaaatg ttcaatactt tcatcaagac gggttgcagg aattattaat agatcctgtc
    18361 gattttgcaa tcagcgattt gccaatcggt tattatccga atgacgaaaa agcaaaagag
    18421 tttttaacaa gtaccgaaga aggccatagt tatgcacatc atttattgct agagcaatca
    18481 atgaaatatg tgaagccgga tggttttggt ttgtttttga tgccgagtgg atttttagaa
    18541 acggatcaaa gtgaagaaat caaaaaatgg tttaaagaag aaggttatct tcaaggaatg
    18601 attcaattgc cagatgaatt gttccgtaat aaacaatcgc aaaaaagtat tttgatctta
    18661 cagaaaaaag gaccacaagc acaacaagta aaagaagtct tgcttgtgaa acttgcttca
    18721 ttaaaagaac cagagaaggt gacggaattt ttcaacgagt ttaaaaactg gaaatcatct
    18781 gctctgtaat cattcaacga gaaggagagt catatgtcta aaacaattgc aatcaatgct
    18841 gggagttcaa gtttgaaatg gcaattatat caaatgccag aagaaaccgt agttgcgaaa
    18901 gggatcgtcg aacgtatcgg tttacaagat tctattttta ccatcaagta cggcgataat
    18961 caaaaattcg aacaaatcat ggatatcgaa aaccacgaag tagcagtaaa aatgctgtta
    19021 gatcagttga ttgaactgca aattttaagc tcttatgatg aaatcaccgg agtagggcac
    19081 cgtgttgttc acggaggaga aacttatggg gattcagtag tgatcgatga gaaagtaatg
    19141 gaccgcattg ctgaattagc agaatttgct cctttgcata atcctgcaaa cttaatgggg
    19201 atcaaagcgt ttaaaaagat tttacctgac attttgagtg tggctgtatt cgatacatct
    19261 ttccactcaa caatgccaaa gcaaaattat ttatatagct taccggtgtc ttattatgaa
    19321 gatttcaaag taagaaaata tggcttccat ggcacaagtc atcgttatgt gtcggaacgt
    19381 gcggcaaaat tattgaacaa accgatcgaa gaactaaaaa tcatcacttg ccacttagga
    19441 aatggtgtat cgatcacagc agtcgatgga ggtaaatcca tggatacttc tatgggattc
    19501 acaccattag ctggggtgac aatgggtaca cgttcaggtg atatcgatcc agctgttctc
    19561 ccatatttaa tggaaaaatt agagattgat attgatgaaa tgatcaatgt tttgaacaag
    19621 aaatctggtc tgctaggcct aacaggattg tctagtgata tgcgtgatct tgaaaagaac
    19681 tatgaaaaag aacatattcg tttagcttat gacattttcg tggaccgcat ccgaaaatac
    19741 atcggcggtt atgtgacaac gatgaatggt gttgatgcaa ttgtctttac tgctggtatc
    19801 ggcgagaatg acggacatgt acgtagtgaa atcatcaaag gtatgtcttg gtttggttgt
    19861 gaaatcgatc cagcgttgaa tgaattgcgt ggtgaagaag tagatatctc tacaaaagaa
    19921 tcaaaagtaa gagtattagt gatcccaaca gatgaggaac taatgattgc ccgtgatgtg
    19981 gagcgtctgc gcggctaagc caaagcattc gttaggataa aagatgaaac gcatgcaaga
    20041 gggaattctt gcatgtgttt tttttgcgga gaagaccgca aaatggtaat ttaacttcaa
    20101 tttcccttct tttttacagg atcttcttag ggaattttgc ttggaaaagt ggtcaaaagg
    20161 gaaaagactg gcattttatt tattgatttt caggaaaatc ccaatatttt attttcattc
    20221 atctttataa gggacattac atatgtaaaa agaaaaaaca tttttagatc agcctcgtgg
    20281 gctgtcgact ttattcttcg ctttatgttt agctagaaaa ccgatccgaa acattcattg
    20341 atcgaataat aaaaacttga ttttccacct gtactttgcc tgaatcatga aagcacaggt
    20401 atgaaaatca agttttttgt tgcgaaaaaa cgtcctagtc aatcagttgg ccgagcggct
    20461 ggcggctgat ctgctcctga tcaataacga gtgcttcttg gaagtgttgc tgcaaaaagt
    20521 agcggtcatg gctgactgct aggagcgttc cttgatagtc ttgcagcatt tcttcaattt
    20581 cttctcgagc atagctgtcg aggtgattgg ttggttcatc caaaatcagg acgttgattt
    20641 tttctaaaaa gagtttcagc aacgccaaac ggattttttc accaccggat aaatctttga
    20701 tccgtttacc cacatcgctt tgataaaagc cacttttggc taattgccgt cgagcgactt
    20761 cttcgtttcc agtcaatgtg atggcgtaag ccagcaaacg gctatcggga tcggcaaact
    20821 gcagctgctg cggcaaataa ccgatttgca agctactacc taatttgatg gtgccaccgt
    20881 aaggcttttc ctcaccgagt aataatttga gtaaggttga ttttcctgca ccattgtcgc
    20941 cgatgatggc taaatgatct ccacgataga ctgcaaaact gctgtctttg aatagtactt
    21001 gatcagcgta ctgctggtgc aagttttgaa tgatgaaaac ttctttgcca gattttccac
    21061 cattagacaa gctttgcagc cgatttttcg ggggaggtgg cggtttgacg agtgtcagtt
    21121 tcgccaggcg gcgttccagt tctttggctt ttttgaagaa ggactcattg tctccttcat
    21181 gtgcccactg acgaaaacga cgaatcatga ctttcaaccg ctggacttct ttttgctgca
    21241 gttcgtaatt ttttgtcagc tcagccaagc gagcttcttt caagactcga aaacggctgt
    21301 agtttccggg atactcaatc aatgcacctt cttccagttc ccatgttttt gtggtgactt
    21361 gatcgagaaa ttgccgatca tgagaaacga ccagaaccga tttttttgta ttctgtaaat
    21421 agttttccaa ccagcgaata ctgttaagat ctaaatgatt ggtcggttca tcgagtaaaa
    21481 gtaaatcggc atcggctgct aaaattttgg ctaaagcgac tttaactcgt tcgcctccgc
    21541 taagttctgt taaccgtgct tcctgacggt gtggaatccc taagccttgc aagatcgctg
    21601 caatccggtc ttctaatacg taaccgccta gttcttcaaa gcgatgctgc agctcaccgt
    21661 aacgggccag acacttctct aagtttgtat ctggttctgt catcaatgct tcgtagcgac
    21721 tcatttgctg tttgaggtcc tgtatctcag cggcagaatg gagcaagtag tggttgacgg
    21781 tcgcttcgga aactgtaagt tcttgttcga ccgtcccgat ctttaagcct tttttgcgac
    21841 tgataacacc gctgtcgaca ccttctctgc cggtcaagat ttgtaaaaag gttgatttgc
    21901 cagttccgtt gataccgact aaggcaattt tttcaccagc ttcaatttga caggataaat
    21961 cctcaaataa tggattcccg ccgattgttt ttttaataga aattgcttgt atgatcatga
    22021 tctctctcct tgagaccact caaaaaggcc atggttatcc atggcctgtg ttatttaaac
    22081 tcagattatc atgagaaaaa taccttgaga tggccattga ttttacgtga attcaactgt
    22141 tttggacaaa cttgtcccgt caacagcaac tgtaaaacca attgcatggc taaatgcgat
    22201 atggtatcgt tggtaatttt cattacttgt tattttagtc atgcgcatca tagtattttc
    22261 cctccttcgt gtagttgtct tttccagtgt acctgaaagt gtcagaaagt tcaactagcg
    22321 ctgacattta cttaatattt atcgatgtgt aattctgtca gagagctttt tttatctgac
    22381 tgagggtaat gtctcgtgga ccacatcatt gtattctgag ctaaaccagc gttcaggcga
    22441 aatcatatgg tgtttgataa aaaactttgt ctacatgact tccttttgcc atttgcttaa
    22501 taccgttgca ttaaacagct cttctctttg gaaatctcac caacatcgcg ttcaggagta
    22561 tcgttgtggt tgcaatggca agcggtagcc tttcatcaat tcttgaatga tttaccgaat
    22621 gtagcacttg ccttctcgcg gtacccgaac ccgaaataaa attttagttt ggcgataaaa
    22681 tcggtatccg ccatcgatac ttcatatttt ttcgtatcag attcatcgga aacttccaca
    22741 ttgacgaacc aataagcttt ggcatgctta ggtcgtttta aaccacacca gggcgactaa
    22801 tcgccctggt gttcactttt ttttatttta cttgaactca ttgactgttt tccctttatt
    22861 ttaaacatta taaagaccga tgagtgcttg ccgcgtcttt tgtttttatc acggtttttt
    22921 gtgcaaaaaa acctgccgat agttggcagg tcgtctacgt gttaagcttt ggtgtggtct
    22981 ttagctggca attggttttc taaatcagtt cggacaacaa gcacatcgca agctgcgttt
    23041 ctaataacat attcagaaac agaaccaata aataaacgtt ctacagcatt caatcctgtg
    23101 gctcctaaca taatcaaatc tacttgatta tcttctggga tttctctggc aataatttgt
    23161 tttggtgagc catattctac gacagaggtt acattattca agccagcttt cttcgcattg
    23221 ctttcataat ctgccaatgt ttgctttgcc atttcagtag cttgttctgc aagcattcca
    23281 tcaaaggatg aaacggtttg gaaagaacgt gtgtcaatga catgtgctaa tagaagttct
    23341 ccgttatttc ggatagctac attgaccgcc tttttaaaag ctaattcagc ttcatcagaa
    23401 ccatctacgg caaccatgat ttttttgtat tgttgtaaca taagcattca ctcctcaagt
    23461 caatcgtatt gtactctttt attgtagaac actttaggga aaaagagaat tatttactgc
    23521 tttggttcag caattttttt tgcaaatcgg atactgaagg tggcagaaac aataattacg
    23581 atcagtaaaa agaccaaggc aataaagcgt tgattgaaaa aagctgttaa aatacccatt
    23641 acgcctaaaa cagcatatgc agctccgtaa aattgtagga atccttgatt tttatcattt
    23701 ttgttgatca atacaaaaaa gatatcctgt ttattaaata aataatagct aacgaataat
    23761 aatactaaag tggcgataat cattaaaatc ctaatcatga acaaatcctc caaaataagc
    23821 aacaaataga aaaagcaatt tgccaagagc aaattgcttt attctttaaa agatggtaat
    23881 ctccgttaat gccgttgttt gtcctgtagt attgatcccg cgaattcaag caggtgggtc
    23941 ccatggtcgt agcttcgaac taccaaatga taaggcatgt taccagcagt gacacaagac
    24001 gggtcccaag atatcaataa aaatgttcgg tcaacacaca aagaggacaa ttcgaacatc
    24061 acagatttcc cacctctata gtagcatatg aatttaaaaa gagcaatgag agagctcgca
    24121 gaaacagaac cgttttcttg tctagttttt tggaggatgc tttttccact cttttatacg
    24181 gtaatactgt tgacccagag cctgttcata tttccctgta tcttttggct gataatactg
    24241 agcattcttc agtttatctg gtaaatattg ctgattgacc caagcctgat caaaatgatg
    24301 aggatactga taacccacgc cacggttgag tgatttcgca cctttgtaat ggctatctct
    24361 caaatgatca ggaacatctc cagctttgcc ctctcgaata tcagcaagtg ctgcatctaa
    24421 tgccatgtag gcagaattgg attttggcga caagcaaaga tcgacgacga cgtctgctag
    24481 tgggatacgt gcctcaggca atcccaattt ttcagcagct aatacagcgt tcactgtacg
    24541 agccgcagca gcaggattac ctaaacctat atcttcataa ccgataacca tcagacgccg
    24601 gcaaatactt gccagatcac ctgcctcaac taaacgggcg agataatgaa gtgcagcatc
    24661 cacatcactt ccacgaatcg atttttgaaa agccgaaata acgtcataat gagcatctcc
    24721 atttttgtca tgggtaagag cttttctttg gacacattct tcaatgatcg acagtgtaag
    24781 atgaatcctt ccttctttat ctgaaggagt cgatagtgta gccagttcca gcccattcaa
    24841 tgcactccgc aaatcgccgt tcgttgcacg cgaaaggtga agaagagcat cttcgtctaa
    24901 ctggattgct tgttgtccaa ggccgcgttc tttatccctt aaagcatgtt cgactgccaa
    24961 ctggatgtct tgttctgtta atggtttgac ttcaaagatc tgtgtacggc tgcggatcgc
    25021 tggattgatg gtaatataag gattctcagt agtcgctccg atcaagatga ttcggccact
    25081 ttctaaatga ggaagcagaa aatcctgttt tgttttatct aatcggtgta cttcatccaa
    25141 taagaggatc actgtaccac tcatttttgc ttcttccgct acaacttgta gatccttttt
    25201 tgaatctgta gcggcgttta gcatgcgaaa tgcataatta gtagagccgg cgatcgcact
    25261 tgcgatactc gtttttccag taccaggagg tccatataga atcatggaag agagcatgcg
    25321 agcatcgacc atgcggcgta tgatttttcc ttccccgact agatgctgct gaccaacgac
    25381 ttcatcgatc gttcgaggtc tcatgcgata agccaatggc tgttgcatag gattttcctc
    25441 cttcttgttt ttacgtccct taattatatc atatttagca agatgcctag aaagaacagc
    25501 cttctgttgt tttgttttca tagcttatcc aactaatatt gtgtatgata aagtaggtga
    25561 gaaaaatgac ggataaacaa aagatagatg aatttttttg gaataaaacg acgccagaag
    25621 tggcacggga tttattgggg atgtatttgg agcatgacac accagaagga agattagctg
    25681 gatatattgt agacgcggaa gcatacctag gtccagaaga cgaggccgcc catagctatg
    25741 gtttaagaag aacaccgcgt gtgcgggcaa tgtatgaaaa accaggtacg atctaccttt
    25801 acacgatgca tactcatcgg attttgaaca tcattactca gccagaaggg attcctcaag
    25861 gagtaatgat acgtgcaatc gaacccgccg caatgattga tcaaatgagt aaaaatcgtg
    25921 gcggaaaaac tggtccagat atcagcaacg gtccgggaaa attagtggaa gcattagcaa
    25981 ttcctcaaga actttatggt cagtctatag cagatagttc gttacgctta gtttttgaga
    26041 aaaaaaagac accaaaaaag atcaatgctt tgcctagaat cggtatccca aacaaaggtg
    26101 tatggacaga aaagccgttg cgttttgttg tgtcgggaaa tccttatatc tctttacaaa
    26161 gaaaaaacca aatcgagaaa gattggggtt ggaggaaaga aaatgaaaaa gaaggatcta
    26221 ttaacatatt tagatgaaat aattgaaaaa caagtgacag actatgatgt agctttagat
    26281 tgggatacaa aaaatcatac gatcgagatc gttgttcgct tgtttgcaga aaataaagca
    26341 caatttgaga ttgatgatgc agaagggatc gtttcgcaag aaccaattat cgaatttgaa
    26401 gacggcgtac ttctttttaa tcctcagaaa tctgtttttg atgaacgcaa ttatctagca
    26461 gtaatccctt atgaaggaaa aaaagggttg gcaaaatcag tagctgattc tttagtggaa
    26521 tatctaaatg aagtattagc tcaagggcaa agcgatttgc ttgatttttt ggatgaagac
    26581 gatgacgaag ctgtattcga gcttcactgg gataatcagc aactagcaga attagtagaa
    26641 gtaaaacagc aaaatgaagc agatacttat ttaccgtacc caagttatta aggagtgaca
    26701 gatatgaaat ggaatgaagt aaaagtagaa actgaaagtg aagcagtcga agcagttgct
    26761 aatattttga tggaggcagg ggcgagtggt gtcgcgattg aagattcttt agatgttgaa
    26821 cattttcagt cggatcaata tggtgagatt ttagataaaa atgattttac atcgctgaaa
    26881 gaaggcgcac tagtcatggc ttattttccg gaaacgactt tcttaccgga aatcttgcct
    26941 tttatcaaag atcgtatcac acaattacct gagtttggtc tagctattgg aaaaaataaa
    27001 gtaacagtta gtgaagtaga ggaaagcaac tgggcaacag cctggaagaa atactatcat
    27061 ccggtgcgta ttacgcgaat gctgacggtt gcgccaagct gggaaaacta ccagacagaa
    27121 gatcctctag aaaaaattat cacactagat cctggtatgg catttggcac aggcactcat
    27181 cccacgactt ccttgacatt gacagcacta gaaacagtct tgcgtggtgg tgaaacattg
    27241 ctagatgtgg gaactgggtc gggcgtgcta agtattgcag caaaatatct tggtgcaaaa
    27301 gatgtctatg cctttgattt agacgaagta gccgttcgtt cggcgaaaga aaatatggat
    27361 atgaatgaaa tagccaaaga tgtccatgta tcagctaacg atcttttgac aggaatcgag
    27421 attgaaagtg atgtaatcgt tgcgaacatt ttagcagata tcatcttatt gatgatccca
    27481 gatgcttggc gtttactgaa gcaaacaggc acgctgatcg tttcaggaat cattgaagag
    27541 aagaagcaaa tggttctgga tgccatgtta gaacaagggt ttatcgtgga tcaaattttc
    27601 cagcaaaaag actggtatgc aattattttg aaaaaaccag aggtggaata gatgcagcgg
    27661 tattttttaa aagaatcata tgaatcaaaa gactattttg ttttagaagg ggaaaactat
    27721 caccacattg tacgggttat gaggatggaa cccgaggatg aagttttttt ggcattcaat
    27781 gatcaaatag ccattgttgc agaaatcacg gagattactg aagaggctgt ttatctgaaa
    27841 gaaattaaaa aagaagaaaa tcaaaaggaa ctgccaagtt cgattaccat agcgagtggc
    27901 tatcctaaag gggaaaagct ggattggatc gttcagaaat ccactgaatt aggagcgcaa
    27961 gcttttcttg gtttcccggc atcagcctct gtcgttaaat gggatgcaaa aaaacgtatg
    28021 aaacgacaac aacggcttga aaagattgct caagaggcag cggaacaatc ccatcggcag
    28081 atcgtgccat cagtagagtt attggaaacg aaacaagatt tatttgagcg gctagatgat
    28141 tatgacgtgg tcttggttgc ttatgaggaa tcagcaaagc aaggcgaaaa atcaaatgca
    28201 gccaaaattc ttagtgaaac aaaaccagat agtcgaatct tagctgtctt tggaccagaa
    28261 ggtggattat cccgcgatga agtcgattat tttgtagaac acaaagggat actttgtgga
    28321 ttaggaccaa gaatccttcg tacagaaaca gcacctcttt atttattaag tgctgctagc
    28381 tatcaatggg aattaatgaa tccttctttc agctaacgtt cttcattaat aaaagagtat
    28441 cgacagaaag aattgaaaaa atagtcaaca atttgtataa tagaaacaat tatttataca
    28501 atggatacga aaaatcgttt gaaaaagctt ttaaaaagcg aacatttccg taaaaaaacg
    28561 tataactgca cggattttgg gacaattatc aaacagatat ttgtcccgat tcccgtttta
    28621 ccgaaaaaag aaagggggat cgattcctgt gacaaaagaa gaaattttaa cgggacccgg
    28681 tgtcataaag ctcgttagtc aatacatggg tcccgaacat gtagcatttg ttgagaaagc
    28741 atgcgaatac gcgactgctg cacatgacgg acagtttaga aaatcaggcg aaccctacat
    28801 tattcatcct atccaagtcg caggtatatt agcagattta aaaatggatc cccatacagt
    28861 ggctacaggc ttcttacatg atgttgttga agatacagaa atcactttag aagatctgag
    28921 agaagaattt ggcgatgacg ttgctatgtt agtggacggc gtaaccaaat tagggaaaat
    28981 aaaatataaa tcccacgaag agcagctagc agaaaaccac cgaaagatgc tgctagcaat
    29041 ggctcaagat ttacgagtta tcatggtcaa attagctgat agactacata acatgcgtac
    29101 gttgaagcac ctgcgagaag ataagcaaag aaggatcgct caggaaactt tagaaatcta
    29161 tgcgcctctt gcacatcgtc tagggatcag ccggatcaaa tgggaattag aagatacagc
    29221 acttcgttat ctaaatccaa aacagtatta ccgtatcgtc cacttgatgc agacgaagag
    29281 agaagaacgt gaaaaatacg taagtggtac tgttgaagat attcgaatag cgacagaaga
    29341 gctggggatt tttgcagaaa tctatggacg gccaaaacac atttattcaa tctatcgtaa
    29401 aatgaaagat cagaaaaagc agttcaacga aatttatgac ttgctagcta ttcgagtgat
    29461 cgtagattca atcaaggatt gttatgctgt attaggagca atccacacaa aatggaaacc
    29521 aatgcccggc agatttaagg attatattgc catgccaaaa gctaatatgt accaatcttt
    29581 gcatactacc gtgatcggtc cggcaggaaa tccggtagaa attcaaatcc gaacacagga
    29641 aatgcatgaa atcgctgaat tcggggttgc tgcacactgg gcctataaag aaggaaaaaa
    29701 tgaaaaagta gaaccagatg gtatgacgaa acaattaagc tggttccatg agatactcga
    29761 acttcaagac gaaagctatg atgcttctga atttatggaa ggcgtaaaag gagatatctt
    29821 tagtgataaa gtctacgtct tcacaccaaa aggagacgtt actgagttac caaaaggatc
    29881 cggaccattg gactttgcat acagtatcca tacagatatc ggtaacaaaa ccactggtgc
    29941 aaaagtaaat ggcaaaatgg tgcagcttga ttacaaattg aaaaacggag atatcattga
    30001 gatcatgact tctccaaatt catttggccc aagtcgcgac tggttgaaat tagttgctac
    30061 tagcaaagca agaaataaga tcaaacgttt cttcaaagcc caagatcgag aagaaaatgt
    30121 gatcaaaggc cacgaatccg tggtcaaatg tattacagat ctaggattta cgcctaaaga
    30181 tattttgacg aagaacaaac tgcaagaagc actcgatcgt tttaattatc aaacagaaga
    30241 tgatctctat gcagctgtag ggtatggaga agttagcccc ttgacgatgg ccaatcgtct
    30301 gactgaaaaa gaacgtaaag aacaaaaaat cgagcagcaa aagcaagaag cagaagaaat
    30361 catgaatcag ccgaaaaaag aacctgacaa aatgaaagta cgtcatgaag gtggcgttgt
    30421 cattcaaggg gtagaaaact tactaattcg tatcagtcgc tgctgtaatc cgattcctgg
    30481 tgatgatatc gttggttata tcactaaagg cagagggata tccattcatc gtcgagattg
    30541 tccgaatgtt cagcctgaca aaccaaatgt agcagaacgt ttgattgaag tcgaatggga
    30601 agatacatcg aatacacgaa aagagtatga tgcagatttg gaaatttacg gctataatcg
    30661 ttcaggctta ttgaatgatg tacttcaaac agtcaatgcg ctaacgaaaa atctcaacag
    30721 cgttgaagca cggacgaata aagataaaat ggcgacgatc catttgacgg ttggtatcca
    30781 gaatttatcc cacctaaaga gtatcgtgga taaaatcaaa gcagtacctg atgtctacag
    30841 tgtacgccgg acgaatggat aggaggacag atatgagagc agttgttcaa cgggtaagta
    30901 aagctgctgt gtcagtcgat cagcaagaaa ttggaaaaat tggctacggc ctacttgttt
    30961 tattaggcat tcatgaaacc gatacgcaaa aagatgtgga ctatctggta aaaaaaattg
    31021 cacaaatgcg tatttttgaa gatgaacaag gaaaaatgaa tattagtgta gaagatgtgg
    31081 gaggacaaat tttaagtatc tctcaattca cactgtttgc ggatacgaaa aaaggaaatc
    31141 gaccaagctt tgtggaagcc gcacgaccag aaacagctat tcctttatac gaatcattca
    31201 atgaaggaat cagaagtcgc ggcatcacag tcgctaccgg acaattcgga gcagatatgt
    31261 cggtttctct cgtcaatgat ggacctgtaa cgatcattat tgattctaaa aacaaataaa
    31321 gtaatctggt tttcactagt aaaaactatg aaacacgtcg cttaagttag actgataaac
    31381 tctaacttaa gtggcttttt tgtctgcaaa aagcgcttct tttgtcagca gtcggaaaaa
    31441 aagttaagct taaattatac aagttatttt ttataggagg ttgctgaaga tgaaagaagt
    31501 accagtagta gatatcaaaa tcacagattt aaaaaaattg tatcaaaaag gatacaacat
    31561 gttggaggag ttacgccatg aagcggacgc tcccgtcgtc aaagcaaaga ttttcaataa
    31621 agaagctatc acgatctacg gatcatcagc agcaaaagta ttttatgatc caaggaattt
    31681 caaacgaaaa ggtgctatgc caaaattagt attgaagaca ctttttggtc aaggtggagt
    31741 acagactttg gatggagctg ctcatcacca tcggaaaaat atttttatgg atctaatgac
    31801 accggaaaga atggaagatt atcatcgtat cttagataag aatctgacac aagcacttga
    31861 agcacagcat ggtcaatttg aactatttga tttaagtaag atggttttct ttacatctat
    31921 ttgcgagtgg gcaggtatca atttatcagc gatttctaaa gatgaggtcg aaaaattagc
    31981 agaatatcaa atctctatga tcagcggaac gtttacttct ccgattgatc acataaaagg
    32041 tgtcgaaaat cgcaaaaaat cagaaaaatg ggcgcaaggc ttgattgaag aagctcgaca
    32101 aaatcctgtg gctggaaaag aaaatgtggc attatatgct tttgccaatg ccacagattt
    32161 agatggtcag cttttacctc tggaagtggc agctgttgaa ttattgaaca tcatccgtcc
    32221 aacggttgct ctaactgtgt gggcggcatt gatgggacat gcacttttca gtagaccaga
    32281 tttgtatcag caattaaaaa atgacttttc gacgttacaa gatccgttta ttcaagaaat
    32341 gagacgctat tatcctttct tcccaatgct acctgcgatt tcgctgaaag aagtagaagt
    32401 agatggttat cgtataccag aaggcagttg ggttatttta gatttatatg gaacagacca
    32461 tgatgaacga acagttgaag cgccagattc ctttatgatc aaacggtatg tagggaaagc
    32521 aaaagatatt tcctataaag aagaatatga gatgattgca caaggcgggg gaaattttcg
    32581 acaaatgcat cgctgtgcag gagaatggat cactcttcat agtctacgtg tgttttccga
    32641 tcagctagta aataagtttg agttctctgt acctgaacaa gattggacga ttccattcaa
    32701 tcagtttccg acttatccga atagccgagc gctactttac aagaattaga ttagcataaa
    32761 cttatcgaaa aaaaggaaga tcaaacagtg ctgatcttcc ttttttcata ttactataaa
    32821 ttgataaggt caattgatca aaatccacga aatgcttcat caatggtttg ataatcttca
    32881 ttggataaat gcaaatctaa agcttggaca ttgtcagata cttgctctgg acgcttagct
    32941 ccaggtataa ctacagacac tcgtggattc ttcatgtacc aagctaaaac aagttgcgca
    33001 ggagtggctt ggtatctttt agcaatgggg cgaagtacat cgacagcggt taaaatttct
    33061 ctgaaacgtt ctccttggaa atctggattt ctttttcgag gatctccttc accaaattgt
    33121 ttttcctcac caagttcata ttttcctgtc agcaaaccgg aagcaagagg aaaataggga
    33181 acaaaactga ttttgttttt ttcaagataa gggaataatt ctttttcagc ctgccgatgg
    33241 atcaaactgt atttatcttc tactacatcg acatatccat ctgcattcgc ttctttcaat
    33301 tgttctaacg tgaagtttga cacaccgact gctcggattt ttcctgcttc ttttaattcg
    33361 tgcaatgtag caactgattc attttttggt gtgctttcat ccggaaagtg gatataaaaa
    33421 atgtcgatgt aatcagtttg cagacgtttt agtgcatctt ccaccgattg tttgagaaac
    33481 tcaggagaat tatcaaatgt tcgcccttta tttggtacat gagcagcttt tgtagcgatg
    33541 acgacacgtg agcgatcata ttctttttcc ttcagtacct cgccaatcag ttcttcggaa
    33601 cggccgtttc cgtaagcata agcggtatcg atcaaattga tcccgctatt caaagctgta
    33661 cgaactactt gttttcctgt ctcatcttcc aaaccgtcaa aaagattatg tcctccaacg
    33721 gcatttgctc ctaatccaag ctgttctgcg tagacttgtg aatgtccgat tcttatttct
    33781 gccatagctg attcctcctt tttgtctagt ctaaaacaaa ataccaaaaa gctgaactaa
    33841 aacgtttttg tgaggggaaa tagtcagata tctcttgact ttgagactgg tttttagtag
    33901 gttataagta atatcgaatg tcaatgaaag agcgagtaag tttttgaaac tttacaagag
    33961 aggatcatcg aggctgaaaa tgatctaaag tttcattagc ctaaagacac tctggagatg
    34021 gacgagtgaa aagccgtccc gcgtacgagc gttattcgta agaggaaaga gaaatctttc
    34081 aaaattaggt ggtaccgtgt atgaatttca ttcacccttg ttcatgatga acaagggtga
    34141 tttttttatt tacatagcta agaaagggag agaaaactat gagtttccaa agaccaaaag
    34201 gaacaaatga tattttgcca ggcgaatcag aaaaatggca gtttgtcgaa gaaacagcac
    34261 gtttattatt cagagattat cagtataatg aaatgcgtac accgattttt gaacactatg
    34321 aagtgatatc ccgaagtgta ggggatacaa cggatatcgt ttcaaaagag atgtatgatt
    34381 tttatgacaa aggggaacgc catgtgacgc ttcgaccaga aggtacagca ccaatcgttc
    34441 gttcttttgt tgagcataaa ttgttcggcc cagagtatgc gaaaccgttc aaaacttttt
    34501 atatgggacc aatgtttcgc tacgaacgcc ctcaaaaagg acggttacgt caattccatc
    34561 aaatcggagt agaagcattt ggatcagaaa accctgcaac tgatgtggag agcatggtga
    34621 tggcacttga tttcttcaaa cagttgggga tcacgcaaat ccggctagtc atcaattcat
    34681 taggtgataa agaaacgcgt caagcctatc gtcaagcatt gatcgactat cttttacctt
    34741 ttgaatcaga gctgagtgaa gactctaaac gccgccttca tgaaaatcca ttacgcgtat
    34801 tggacagtaa agacaaacga gatcaaaaat ttgtggcaga agctccatct atcttagatt
    34861 atctaagcga gcctgctcaa aaacattttg ataccgttgt ttccatgctg gatgcattgg
    34921 ctgttcctta tgaaatcgac agtaatatgg ttcgcggact agattattac acacatacga
    34981 tttttgaaat catgagtgac gcaccaaaaa tgggggcaca agcaacgatt tgcgcaggcg
    35041 gacgttatga caatctagtg gaagaactag gtggtccttc aacacctggc ttcggatttg
    35101 caatgggtat cgagcgatta ctcttgacta tggaggcaga ggaagtagtt atccctgcat
    35161 ttaatgagtt ggatgcttac gtggtcgcac tcggagacga aacaaatatc gaagcattga
    35221 aagtggtcca agcgattcga aactttggtt tttcagctga ccgcgacttc atgaaccgta
    35281 aagcaaaagc gcaatttaag acagcagata aagcgaatgc caaattagta ttggtgatcg
    35341 gtgaagatga gcttgctagt caaacgatta atgtcaaagc aatggcaagc cgaaaagaaa
    35401 aaagttttcc attagaagag atatatgaac attttgatga agtctatgat gaaatgactt
    35461 tattcgagta ggaggaaaaa aataaatggc acaaagaacg atctattgcg gattagtttc
    35521 cgaacaacaa ttagaacaaa cagttacatt aaaaggatgg gtacaaaaaa gacgagacct
    35581 tgggggagta atctttatcg acctacgtga tcgtgaagga atcgttcaaa tcgtctttaa
    35641 tcctgaaatc aacaaagaag catgggaaat cgcggataaa tgtcgcagtg aatacgtgat
    35701 cgaagtgact ggggaagtta gaagacgcgg agaaaatgca gtaaatccta aaatggccac
    35761 tggtgaattc gaagtgattg caagtgaaat cgtgattttg aatcaagcaa aaaccccgcc
    35821 atttcaaatt gaagatgatc aagcaatcag tgacgaaata cgtttgaaat atcgttattt
    35881 ggatctgcgt cgtccaaaaa tgactaaaaa catcaaatta agaaatgcga caacaaaagc
    35941 gattcgtcat tatttagatg ataatgactt tctcgatatc gaaacacctt acctagggaa
    36001 atctacacca gaaggcgcaa gagactacct agttccttca cgtgtccatg ctggacattt
    36061 ctatgcgttg ccgcaatctc ctcaattgtt taaacaattg ctgatgaatg ctggattcga
    36121 ccgttattat caaatcgttc gttgtttccg cgatgaagat ttacgtggag accgtcagcc
    36181 tgaatttacg caagtagata ttgaaacaac gttcttaaca gcagaagaaa tccaaactta
    36241 cacagaggga ttgattgcga aggtaatgaa agaagttcgc ggtatcgaag tcacattgcc
    36301 atttcctcgt atgacctatg atgaagcaat ggctcgctac ggcagcgaca aaccggatac
    36361 acgttttgat atggaattga tcgatctaag cgatacagtc aaagaagtag aattcaaagt
    36421 cttccaaatg gctcttgaaa acggcggtgt cgtgaaagca ttgaatgcta aaggcgctgc
    36481 agatcgctac tcacgaaaag acatggatca gttaggtcaa tatgtaggac aattcggagc
    36541 taaggggctt gcatggctga aagtagaaga agacggattg aaaggtccaa ttgctaaatt
    36601 catgggagaa gcaacagaag caatcattaa agcaacagat gcaaaaccag gtgacttgct
    36661 gatgtttggc gcagataaaa gtgaaatcgt cgctgctgca ttaggtgcta tccggacacg
    36721 tttaggaaaa gaattaggac taattgatga atcgaaattc aatttcttat gggtaactga
    36781 ttggccacaa tttgaatttt cagaagaaga aggtcgatat gtctctgctc accatccatt
    36841 cacgatgcca aaagaagaag atatcccgtt attggctgaa gaccctgcaa aagtctatgc
    36901 agaagcttat gatatcgtct tgaacggtta tgaactaggc ggaggttcta tccgtattca
    36961 tactcgtgag cttcaagaaa aagtactaga aactctaggc tttacaaaag aacaaatgga
    37021 agaacaattt ggtttcttgt taacagcttt agattacgga ttccctccac atggcggtat
    37081 tgcattagga ttggaccgtt ttgcaatgtt attagccgga gaagataata ttcgtgaagt
    37141 cattgccttt cctaaaaacg gaaaagcagc agatgtgatg agtgatgcac caagtatggt
    37201 ttcgccttta caattgttcg aattgaatat tgatgtaacg aatatagaag aatagccgat
    37261 aaaaaggtaa aaaggatttg tcgaatacgc ttcttttgac aaccttgatt taattgaaaa
    37321 acactttata atgaagccat aacatataaa gcggggcttt tccatgaaaa aaacattcga
    37381 aaattgggct tatcatgatt acacgaccaa gctttcggta tctatcgttt atgcgttgat
    37441 cgcatcagtc gcattgaatt tcttttatca gccaggacat atttattcaa gtggtattac
    37501 tggtctagct cagattttga cgactttgtc aaaatcactg ataggattcg aagtaccggt
    37561 atcaacaact ttgtatttac tgaatatacc tttgtttgta ttggcgtggt taaaaatcgg
    37621 gaagaagttt acaattttca cttttatcac agtcactttg acctcccttt gtatccatat
    37681 tttaccggaa acagtcctat ctaccgatcc aatcatctgt gcgttgtttg ggggggctgt
    37741 gatgggacta ggtattgggc tagcgttgag aaacggccta tcatcgggcg gtctagatat
    37801 cgtgagtatc acgattcgta aaaaaacagg aaagtcgatt ggttctattt cgatctactt
    37861 caatgcattg atcgtttttg ttgccggaat tctatttggc tggcaatata tgttttacag
    37921 tacgttgtct attttcgtca gtggaaaagt aactgatgtc gtttatacta aacaaaagaa
    37981 gatgcaggta atgatcgtaa cgaaacatcc agaaaaagta gtaaatgaaa tccaaaactg
    38041 tatgcgtaga gggatcacga tcatcaatga agcagaaggt gcttataagc atgataagca
    38101 gacagtattg atgacgatcg ttacacgata cgaactgcct ctacttagag aagcaatgaa
    38161 agcttctgat ccacaagctt ttgtaagtat tgcagaaaac gtccagatac ttggtcgttt
    38221 ctatgaggat ggcatttaga tcgagtttga caaaaattca tgaaatactg ctgtagacag
    38281 acgttcgttt gtctgcagtt tttttctacc atagtagaat cagcaaagaa aaactgcttt
    38341 tttatttgaa attttcttaa agttgaaaaa aagcatgttt atttttttaa atttatggga
    38401 tgatagaaaa aaatagtaga gaaaggacac gattctactt gctattttca cttaataccc
    38461 gtatattacg aatgaaaaaa gtttatgaaa agttggaata ttgaatggaa aaacaaaaaa
    38521 ggttaaaaaa cagccgctac atcactgggt ttgatgggct ccgttcatta gctgtggtgg
    38581 gtgttatctt ctatcacctt ctgccaacta gtatgaaagg cggctatttg ggcgttccga
    38641 tttttttcgt cgtatctggt tatttgatca cggatctgct gcggcaggaa tgggaacaaa
    38701 atggaaaaat caatatttgg caattttatg tcagaagaat gaagagactc tatccggcac
    38761 ttgtttttat gcttgtgaca gcttcggctt atatcacttt gtttcagcga gggctgttaa
    38821 acaatcttcg aggagttgtg gtcagcagct tactgtatgt caacaactgg tggcaaatca
    38881 ataacggttt atcttatttc gatcgatttg ctaatgagtc accgtttacg catatctggt
    38941 ctttagcagt agaaggtcaa aactatttgg tttggccgat cttgtttgta ttgttgatgg
    39001 tatttgttcg taaaagaaaa tggatctttt atactgtgtt aggtacatcc ttcctctccg
    39061 ccatcttgat gatgatcttg tatacaccag gaggcgatcc aacccgtgtt tattacggta
    39121 ccgacactcg tctgttttct atatggttgg gaagtgcgct tgcgtttgtt tggccaagta
    39181 cgcgtttaaa gaaaaacatc ccgaaacaag cgaaaaatat tctgaatatc gctggaggaa
    39241 tctcgctttt ggcactgatt ctttgcttct tctttttaga tgatcattac aatttcatct
    39301 attacggagg aatgctccta gtgagtctgt tatgcgtagt gctagttgca gtgacagcac
    39361 accccggagc aagtttgaat cgatggctga caaatccagt atttacttgg attggcaaac
    39421 gaagctatgg catttatctg tatcagtttc cagttatgat tttttatgaa gcaaaggtga
    39481 ctgatattgc cgatcatgtt ttgttgcata ctttagtgga aatcacattg attctaatca
    39541 tcagtgaact ttcttatcga tatgtggagc gtccgctagc acgttttgat tatggtcgta
    39601 cattccaagt gatgaaagga tggttcagca aaccgatcat cagtaagagc aaaccatggg
    39661 ttgttccagg cacattactt gtagcaattg cactcgttgg tttcgttacg gcaccgaaaa
    39721 acgctgtaac agccgatcaa aaacaactgc aggcaaaaat cgctgaaagt aaaaaattag
    39781 cagaagaaac cagaaacaat tcagaaaaca ccactagttc ggttgaccaa gcgatccttg
    39841 ataagtacga gttgtccaag gcacaaggga ataaggcaca aaaattagaa ctaacggcat
    39901 ttggtgattc agttatgttg gatgctgcaa cggatctaca agaactttat ccaaaagtag
    39961 tggtagatgg agatgtcgga cgacaattat atgccagtgt accgtacatt gaaaaattaa
    40021 aggaacaaga tttgttgaaa gacactgttc tgatcggact aggaacgaat ggctcattct
    40081 cggaagctca gtttgatagt gtgatggatg caatcggtga tcgaaaagta tactgggtca
    40141 atgtccgagt accgacgaag cgctggcaaa atgacgtgaa tgcgatgctt gaacagatga
    40201 cgaaaaaata cgacaatatg acgctgattg attggtacag ctacagtaat gaccatagtg
    40261 actggtttta tgatgatcaa gtccatccaa atcctgaagg gatgacacat tacattcact
    40321 tagtcagcga agccttgcta ggaggaagtt caagtacgaa cagcagtgag acaaacgtga
    40381 cagaatgata gctacaaaaa acttcaggca aaaacatttg tttttgcctg aagttttttt
    40441 atatttaccg tgtttttaac attcatatag attcaggaag caaaacggtt gagtgtgtct
    40501 gtaaataacg ttctttttgt tttcgggtga gttttttgaa tgcagcttct gcttttgttg
    40561 cttcgcttct tgaagaaaat acttctgcat ggatcatctt aacaggtctt cttttttgaa
    40621 gacgagtata tttagcacca gtaccattgt tatgttcctc taatcgtcta gaaagatcgg
    40681 ttgtatatcc accataaaat gtctgatctt tacagaataa tacgtaaaaa taatgttcat
    40741 ttgccataga gcatctcacg aatctctgca gtgtattctc catccatatt gtaagtgatc
    40801 aatggtggag cgactttgaa tccgtctaat ttgccttctt tgattccttc aactaacaaa
    40861 atatttgcct cttttccagg ttttgggtaa acaaattgga tccttttagg tgcaattcga
    40921 tagtcttcca tgacctgaat gatttctaag aaccgatcag gacgatggac catagtaaag
    40981 cgtccatttg tttttagtag cttacttgaa atatcgatca cttcttctaa cgtcgtatga
    41041 atctcatgac gggcgattgc caggtgttta ttagggttcg ttttatttgt tggcagccct
    41101 ttgaaataag gtggattaca gacgactaag tcacaagaat tatgtcgaac gacagataag
    41161 ctgtctttca gatcgatggt gtggacagtc atttgttcat ttaaatggtt aagcgcgata
    41221 ctacgtcttg ccatgtctgc tagacgaggt tgaagttcga tagcgtcgat agccgcctgg
    41281 gttcgtttac ttaaaaaaag tccaactgcg ccatttcctg cacaaaggtc aacaatcttc
    41341 ccttttttag gtacacggga aaagtgagca agcagcacag catctaaaga aaaagaaaac
    41401 actttggaac tttggataat cttgatatct tctgcgtata attggtcgat tcgttcgtct
    41461 tcgtgaagca tggattccct ccaaaatgaa aaaaatagtc gtctgtatta tactattttt
    41521 attaaaaaga accaaggtgg cttgtcaaac ctctattttt tatgcatact atgaccataa
    41581 gaagggaaag gaagagacta tgttttatcg atttatgcgc ggggtagttc gagtactatt
    41641 agccttagtt aatggaaatg cccgctatga gaataaagaa gttttaccgc aagatgagaa
    41701 ttacatacta gttgctcccc atcgtacttg gtgggaccca ttatatctgg cggtagctgc
    41761 agcacccaag gaattcagct ttatggcaaa agaagaactg ttcaaaaatc cagttctgcg
    41821 atttatatta actcgatcca atgcttttcc agttaaaaga gaaaatccag gaccaagcgt
    41881 cattaaaaca cctgtaaaga ttttgaaatc gacaaatttg agtttgatca tgtttcccag
    41941 tgggacaaga catgctacag aacttaaagg cggaatggca ttgatttcca gaatgtctaa
    42001 agcgaaaatc gtgccagctg tatatcaagg tccgttgaca cttggggatc tgttcaagcg
    42061 tcagagagtg acagtgcgtt tcggcgaacc aatcgactta tcagatatca agaaaacgga
    42121 taaagaaggt atggcggaga tcgaacgacg aacacaagca gcttttgaca aattggacaa
    42181 agaagtgaac cctgatttca aatatgaagt caaaaaaaaa taaaatgata agtttatgcg
    42241 tgggcggtct tttgaatcta ctgtcctgtt cagttcagac tttttcaatg aaaacgaggg
    42301 agagacgtca agtctgacct ctttttgcgt tccaatatgt aaaatagaag acaggtgtca
    42361 tgtaatggat caaagtgtta taataattag aaggacattt tttgaggtga ttgaatgcgt
    42421 tttttacata cagccgattg gcatatcgga aaaaaactac aaggttatga cttgattgaa
    42481 gaccagcgtc atgtactatc ttctatacta gatatcgcga aagaagaaaa agtagaggca
    42541 atcgtgattg ccggagactt atatgatagg agtgtcccag ctgttgatgc cgtagaatta
    42601 tacaatgaat tgatggtcga ttggaattta aaggaaaaga tcccgatttt tgctatatca
    42661 ggaaatcacg atagcagtac gagactttca gcgggtacgc catggttcag ttattcgaac
    42721 tattttttgc gaacacagct agcagaagca tttgaaccag tcgagtttaa aaacgtacaa
    42781 tttttctttc ttccttactt tgaaccaatt gcagcgagat tatattttga agatgacaat
    42841 atccgaacga tcaagcaagc ggtagccaag gtgatagata aaatgcagga gctatttgat
    42901 tcagaaaaaa aacaagtatt gattagccat ttttttgtaa tgggaagtgt aaaatctgat
    42961 tcggaaacga aaatcgaagt aggcgggttg gattcgatcc ctggtgaact acttacaccg
    43021 tttgattatg tagcactagg acatttgcat aatcaagctg ctttgagaca ggataatgct
    43081 cgctatagcg gttccccatt gaaattctct ttatctgaga tcaaccaaaa aaaaggcgta
    43141 tggatcgttg acctgacaac aggcctggag ttgaatttta gggagatcga acccctgcat
    43201 gatattgaag aaataacagc gagtttcaaa gaattgctgg atcctgcctt ttatcagtcc
    43261 gtcaatcggg agaattacat ctatatctat ctcactgata gaacgatcat cccgaatatg
    43321 atgaaccagc ttcggcaaat atatcctcgt attttgggga ttgaacgggc aaacggtcga
    43381 gcagaacaaa caaagcgcaa acgaaaagta ctagaaatga aagatcctca aaaattagca
    43441 gaagcatttt ttagtgaaat gacggaagag cagctaactg caaaccaaga aaaatggctc
    43501 ggagaaacgt tgcaagaaat catggaaatg gaatgatcaa gaatgaaacc gaaaaaatta
    43561 caacttaaaa attttggtcc ttttatcaat gagaccgtag atttttcacg tttgacagaa
    43621 gcaccgcttt ttttgatcag cgggaagaca ggagctggca aaacgacgat ttttgatggg
    43681 atcaccttcg cactttttgg tgaaacttcc ggaagacttc gctctggaaa agagatgcgt
    43741 tctttgtttg ctacaccaga cgaagaaaca agcgtgactt tttcttttga acatcagcag
    43801 atgagctatg aaatcgaacg aaaaccagaa caagttttag caaaacgaaa aggaaacgga
    43861 acaaaaaaac aggcagccaa agtaacgttg accatttttg atgggaaagg aaaagaaata
    43921 aaacaaatca caaaacgtac agaagcagat cagttgatca gagaactgat gaatctcgat
    43981 gcgaaacagt tttctcaaat cgttttacta ccccaaggag aattccgtaa ttttttggtt
    44041 tcatcaagca gtgataaaga gactgtttta cgtcatttat tcggtactca atttttccaa
    44101 cagttcaatg agcgattaaa agaaaaagca aaacaacagc aacaaaaatt ggatcattta
    44161 gagcaagagc tgcatcttct gcaaaaaagg tttgttttag tcaaagaaga ggaaccagca
    44221 gcagggtttg aagccgtttt agcccaatgg acaaaccatc aggcaatact cgaaaaacaa
    44281 atcacggaag aaaaagaagc tctaacggaa cttcaggaaa aacaaaagca actagaaaat
    44341 tcctactatc gttttgaaag cttgaaaaaa agttatgaag aaaaaacgat attaataaac
    44401 aagcaaaagg aattagctgc tcaaaaagac gaaatagagg aaaagaaacg atggattggg
    44461 tattatgaat ttacgcaacg attagtcgaa ccaatccagc gcctacaaga aataaaagtt
    44521 gaaaaaatag aactgtcaca aaaaagtagt gagcaaacaa agaaactagc gatgactacg
    44581 cagaattatg aagagtggca aaaaaatgaa gcaaaccgcg tacgccgaca aacaaaaatc
    44641 aaagaagctg ctcaaaaact gcaaaacaaa cagatgatgg tgccaattgt cgaatcctta
    44701 cgtagtaaaa aacaagacgc agaacaatta acaatttcat tggataaaga agaattgaat
    44761 gccgagaagc tgtatcaaaa tctcgctatc tatcaggaaa gaactcgaat tcttcaagaa
    44821 aatttactgg atcaggaaaa actgcaagaa gatcgtcttg cttttggaca gttaaaatat
    44881 ctagatgaaa agcagcaaga aatagaaaaa caaaaagcaa aagacactgg gtcgctcgca
    44941 cagataagag aagaactcaa aaaagcaact gaagaaataa caaaaaatag tcagctattg
    45001 aaagaacaga cagaacaagc agaacagctg aaaagccaat gggcaaagca gcaaattgct
    45061 cgtcttcagc tttttttgcg tccaggggag ccatgtcttg tttgtggatc aacggaacac
    45121 cccatacaac aattaaaaca cgaacagcca aataaggaag atatcttaaa agtagaacaa
    45181 caactggcag aagctgagtc tgctgtagaa agaactcagc aaatcattgc caaaagtgaa
    45241 gcacatgaaa ataatcttca acaacaatta tcagatttag aacaaaaaat catggcaacg
    45301 aatatacaat tggctgaaca agaagaacac ttgcaacagg ctttgaaaaa ggcatttcct
    45361 gagataacag aacaaaaaac aaaaacaact cttgtagtaa tcgaacaatc tttagataga
    45421 cgaaaaagca agatggccaa tgcaagaata gagctggaag aaataagaca acaagcagaa
    45481 aagctcgaac aacaattagc tgtctacaaa cagcagcaga cagaaaaaaa taatcagctc
    45541 cagcaactgc aaggagaaat caaaagttta gaacaacaat tgcaagatac ggatagtaac
    45601 tgtttagaag aggagatacg acgacttgaa aaacagttag aagaagactc gaatcaacta
    45661 gctgttgagc agaaagcagg agtgcaattg ttgcaagatc ttcatacgct tcggacacag
    45721 cttgatcttc aacatgaaca aatagaaaaa atagatgaaa aaatagaaaa ggaacaagaa
    45781 agaatagcta aaaagttaga aagccagtcg tatttttcaa caattgaaga aatctgtgca
    45841 tttgcacaac agcaagcaca atataaagaa aaccaacgta cagttacttc ttatcatgaa
    45901 gagcaattag tgacagctga ccggctgcgt caattagaaa atatccagct aactgagaca
    45961 ttcccagata cagaagagat cagccaacag ttagaagaaa taagacgtat gatcgaaata
    46021 aagcaagaaa agatatacgg tttacaagaa caaaaaatta gtaatcaaaa aatctacgat
    46081 gaatgtcttt cgatttatca cacaagccag acgaaattag atgagcttac gcaattacag
    46141 cagctttcgc aaacattcaa cggagaaaat cctaagaaga ctagtttgga acggtatgtg
    46201 cttcaggttt atcttcaaga agtgttacaa gtagcaaatg atcatctcca acgtttaacg
    46261 aaaaatcgtt atcaatttga gctagcggat accattggca gttatcgagg gaaaaccgga
    46321 ttagaaatca atatctatga tgatcaggca ggtatgtctc gaagtgcgca taccctgtct
    46381 ggcggtgaga gttttattgc agcactttct ttagcattag ctcttgccga tgtgatccag
    46441 acacaagctg gtggtgtagc aatcgatgca ttgtttattg atgaaggatt tgggtcatta
    46501 gatgaagaag ctttggaaat ggcaatggaa gctttagaaa cgatcgaaag tgaaggaaga
    46561 atgatcggca tcattagtca tgtaagagaa ttgaaagaac gagtattaca acaaataaga
    46621 attgatacag aaggtagcgg acaaagcaaa atacgctatc aacttggata aaaagggaga
    46681 ttggtaacta tgaaatggag tattccagaa aaagtcattg cacgtggacg tcaatacttg
    46741 caagaaggcc gagtgttatc ggttgtccct gaccaagaag aaaacgtgtg gcatgcagaa
    46801 gtcttgggca gtgaattgta tatggttgat ttggatggta cagcaaaaga gatagattac
    46861 tgccaatgtc cctattggga ggaacataaa tattgtaagc atacagttgc tgtggaactt
    46921 tatttacggt cgaaaaatct atctcgtatc atgaaaaaag accagccaat gctagaaccg
    46981 gtaaaagcaa caagtgaggg agaaatcatt gcaaaaggtt tttctcgtct aaaaacacaa
    47041 aacaatcctc aagcggtgca acctttgatt attgaatacc aagtagaaac gattgagact
    47101 aatcagtatc acccagagct ttctattttg gcactttact tgcgtatcgg ttcattggca
    47161 tcaccgaaaa aaacatatat cgtaaaaaac atctatgatt ttttacaagc ttacacagaa
    47221 aaagaaactt acactgttaa taaacagtac cagtttcgtc tagataaacg ggcgtttcaa
    47281 aaagaagatc aaaaaatact cacgcatctt gcaggaagtg ctcagacgca tcaattatta
    47341 ggtacgaacg gcttacaagt caaaggaaaa ctagataaaa gatatctgct tctcccagta
    47401 gaacaagccc gcttcctgtt agagcagatg aatcagacta cacgtttgca tctgcagata
    47461 aatgaacaaa acgatcaagg aatacaattt tccgatcaaa acaaaccttt atcattcaaa
    47521 gtggaaaaat ttggagaaga ttatttgctg caagcattga atgattttga ttttttcttt
    47581 atgcattatc agtggggagt tatccaaggg acattttatt ctctgacgaa atatcaacaa
    47641 acggtttatc aaacttggaa acaattgatt cgccgattgg agaaaccaga agtagtgttc
    47701 aagaaaaaag aattaccgtc tttgtttaaa gagattatcc ctctgctgtc agagatcggc
    47761 gacgtagcta ttgcccctgc agtagaagag gaagtagcag atatccccgt cgaatttatt
    47821 ttctttttcc gaaaagcaaa aggaaaaatc caagctcgag tcgatttcgt ctatggcgaa
    47881 gtcatctatt cgactgaccc gaagcatgag gtctcgacag aacaggcttt tcaggtgtta
    47941 cgtgacacag tagaagagaa acgagtactt gatctattca gaatgtatcg ctaccagaaa
    48001 aatgaaactg gttatgaacg tccattgcca agcggggaag aactatatgc ctttttccgc
    48061 acagaactat ctgtcttccg tcagtttggt gaagtcagac ttggaagaaa attgcgagag
    48121 ttgtatttag atgcgcataa acatcaaccg aaaatcgaag taaccgagac cggttcatgg
    48181 ctcgatattc gattcgatgt gacagggata gaggagcaag aaatcgatca tgtcctgcaa
    48241 agcttgctga gaaatgatgc tttttataca ttagaaaatg gtcaggttct ttcttttgat
    48301 tcagaagaat ttcaacaaac aagccaagtg ttgcaacaat tacgggaatc gatacgtaca
    48361 gaagaaggaa cgatccatgt acctaaaaat caaggattga tcattcaaaa tcaattggaa
    48421 aaaagcaatg ctacttttag tgaatcgttc caaacgatgg tacaagatct cattcatcca
    48481 gaaagatacc aggcacagct tcccaaagga ttgaatgcaa cgatgagaga ttatcaaaag
    48541 caaggttttc gatggttgaa gatgttaggt cattatcagt ttggcggtat tttagccgat
    48601 gaaatgggac ttgggaaaac actacagaca attgcctttt tactttcgga aaaagaagaa
    48661 cggaaaagct tctctgcttt gatcgtagcg cctgcgagct taatttacaa ctggcaggca
    48721 gaggtgagaa aattcgcccc ttctttaagt atccaagtaa tcaatgggaa taaaaaagaa
    48781 cgtgaagaat tactggctaa agacacagat atccgagtga cttcttatgc cagcttaaga
    48841 caggatctag ctaattatca gtcgcaaaaa attgattatt tgattcttga tgaggcacaa
    48901 atggtcaaaa atagcagtac gaaaactgca caagcattga gagaattagc tgtacctcag
    48961 cggtttgcac tgagtgggac accaatcgag aataatttag aagaattgtg gtcattgttt
    49021 gcaacgatta tgccaggatt tttcccgact aaaacaaaat tcagggaact tgccgcagaa
    49081 gaaattgcgc aaatgattcg accgtttatt ttacggcgag ataaacaaac tgtgttgaaa
    49141 gacttgccgg aaaaaacaga gatgaatcta tattctgcac ttacagaaga acaaaaaact
    49201 gtttatttag cttacttgcg tcaaatgcgt gaagaaatca catcaatgga ttctgaagca
    49261 ttcaagaaaa accggatcgg gatacttgct ggtctgacaa gactgcgaca aatttgctgc
    49321 gatcctcgtc tgtttattga agattaccaa ggcggttccg gcaagctcga acaagtaaaa
    49381 gatctactgg tagctgccaa agagaacaaa cgaagagttt tgctgttttc tcaatttaca
    49441 gggatgctga ctatcttgca agaagagtta gccgagctag ggatctcgac attttattta
    49501 agaggcagta cgaaaccaca agatcggtta agtatggtag acgcatttaa tgctggcgaa
    49561 aaagatgttt ttctgatttc cttaaaggct gggggaactg gattgaatct cacgggagca
    49621 gatacggtca tcttgtatga cctttggtgg aatcctgcca ttgaagaaca agctgctgga
    49681 cgtgcacatc ggatcggaca aaaaaatgtg gtagaagtgt ggcgtatgat ctctgaagga
    49741 acgattgagg aacgcatgga ttcactccag caagaaaaac gcgaactgtt ccaaaaagtg
    49801 atccaaggta atgaagaaca gttgacgaaa ctgactgaag aagacatccg catgatttta
    49861 agtgtaggag aaatcgagga gtaagagact cgaaaaagcg ttcagtaatg ggtaagaaag
    49921 cctttctaaa gaagcgattg tttatttgat ttaaaaaata gcaaggaggc tgggattcca
    49981 ttttatccca gcttccttgc tatcattttt ttatttctat ttttaaaggt tatacaaaga
    50041 ttgtcttcat tgtctctaac agctgatcaa aagaagaaat ctcgatatcg caaattggag
    50101 tctgttcaat tttgcgttct cgatggttga accaaagcgc ttgccagtta gcactttttg
    50161 cacctagaac atcattgtcg taattatcgc caacgtataa ggtctcttct ggaagcatat
    50221 gaaattcttt ctcagctagc tgaaaaatct ctttttctgg tttttggaaa cctgttgctt
    50281 gagaaatgag catattgcgg gaaggaatcc aattgttcaa ttgaagttgc ttcagttttt
    50341 ttgtctgatg gtcagtcggc ccatttgtga taattcctaa tgggacattc ttttccttca
    50401 agaaatcgag ggtttttttg acctcctcat gcaagcagat attatcaagt tcttcctcgt
    50461 aaattttttg aaataagagg ccatcttctt ccgtaatatg agggtagtct aaatctttta
    50521 gtgattggct gatccgatgt gctcgcatat attctagtgt ccaatctcct gccattactt
    50581 ttgggaagtt ttcatcacta tgatggcgga accggatata tagtggatgc atatctgcat
    50641 ctgaaacaag cggaacgact cttttgaccg cgtttcgaaa tggctgctgc tgatcataca
    50701 tcgtatcatc aacatcaaac acaactgatt taatcaaaat tttacacgcc tttcggtttt
    50761 cattgatttt ttcacaaatc cttgactatt gtaacagaaa aaattgtcta aaaaagctga
    50821 taaataaagg tttttcttac tatatttcta tttccttgac agaataaggt ttttatattt
    50881 cagttttttt cactaaaaat cgtgaaacac gtcaataagt ttgatgaaca aattaatcat
    50941 tatggtagaa gtcgattgat ttattttaat gcaatctctg tggaattttc gaaagattta
    51001 tttttttaat acagtctcaa tataactatt caaacataaa ataacgttat ttgatttatt
    51061 tgtttatagt aaatttta
//
LOCUS       DAIGOK010000004        50721 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.004, whole genome shotgun sequence.
ACCESSION   DAIGOK010000004 DAIGOK010000000
VERSION     DAIGOK010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.004"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(86..1450)
                     /locus_tag="NOA01_000148"
     CDS             complement(86..1450)
                     /locus_tag="NOA01_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCI0631387.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLFEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(1447..3006)
                     /locus_tag="NOA01_000149"
     CDS             complement(1447..3006)
                     /locus_tag="NOA01_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCI0631388.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(3062..5542)
                     /locus_tag="NOA01_000150"
                     /pseudo
     CDS             complement(3062..5542)
                     /locus_tag="NOA01_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5526..5861)
                     /locus_tag="NOA01_000151"
     CDS             complement(5526..5861)
                     /locus_tag="NOA01_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631389.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(5875..6909)
                     /locus_tag="NOA01_000152"
     CDS             complement(5875..6909)
                     /locus_tag="NOA01_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HCI0631390.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(6999..7679)
                     /locus_tag="NOA01_000153"
     CDS             complement(6999..7679)
                     /locus_tag="NOA01_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCI0631391.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(7963..9066)
                     /locus_tag="NOA01_000154"
     CDS             complement(7963..9066)
                     /locus_tag="NOA01_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCI0631392.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(9695..10165)
                     /locus_tag="NOA01_000155"
     CDS             complement(9695..10165)
                     /locus_tag="NOA01_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HCI0631393.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            10495..12135
                     /locus_tag="NOA01_000156"
     CDS             10495..12135
                     /locus_tag="NOA01_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HCI0631394.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            12289..13611
                     /locus_tag="NOA01_000157"
     CDS             12289..13611
                     /locus_tag="NOA01_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HCI0631395.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(13679..14113)
                     /locus_tag="NOA01_000158"
     CDS             complement(13679..14113)
                     /locus_tag="NOA01_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCI0631396.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(14178..15014)
                     /locus_tag="NOA01_000159"
     CDS             complement(14178..15014)
                     /locus_tag="NOA01_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCI0631397.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(15756..16382)
                     /locus_tag="NOA01_000160"
     CDS             complement(15756..16382)
                     /locus_tag="NOA01_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631398.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(16677..17012)
                     /locus_tag="NOA01_000161"
     CDS             complement(16677..17012)
                     /locus_tag="NOA01_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCI0631399.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(16999..17283)
                     /locus_tag="NOA01_000162"
     CDS             complement(16999..17283)
                     /locus_tag="NOA01_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCI0631400.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(17339..18862)
                     /locus_tag="NOA01_000163"
     CDS             complement(17339..18862)
                     /locus_tag="NOA01_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCI0631401.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(18855..20030)
                     /locus_tag="NOA01_000164"
     CDS             complement(18855..20030)
                     /locus_tag="NOA01_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCI0631402.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(20034..20219)
                     /locus_tag="NOA01_000165"
     CDS             complement(20034..20219)
                     /locus_tag="NOA01_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631403.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(20185..21879)
                     /locus_tag="NOA01_000166"
     CDS             complement(20185..21879)
                     /locus_tag="NOA01_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCI0631404.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(21876..22349)
                     /locus_tag="NOA01_000167"
     CDS             complement(21876..22349)
                     /locus_tag="NOA01_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCI0631405.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(22418..22573)
                     /locus_tag="NOA01_000168"
     CDS             complement(22418..22573)
                     /locus_tag="NOA01_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HCI0631406.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(22707..23087)
                     /locus_tag="NOA01_000169"
     CDS             complement(22707..23087)
                     /locus_tag="NOA01_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCI0631407.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(23091..23306)
                     /locus_tag="NOA01_000170"
     CDS             complement(23091..23306)
                     /locus_tag="NOA01_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631408.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(23309..23716)
                     /locus_tag="NOA01_000171"
     CDS             complement(23309..23716)
                     /locus_tag="NOA01_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HCI0631409.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(23982..25457)
                     /locus_tag="NOA01_000172"
     CDS             complement(23982..25457)
                     /locus_tag="NOA01_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCI0631410.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGESTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(25447..26295)
                     /locus_tag="NOA01_000173"
     CDS             complement(25447..26295)
                     /locus_tag="NOA01_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HCI0631411.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(26332..26694)
                     /locus_tag="NOA01_000174"
     CDS             complement(26332..26694)
                     /locus_tag="NOA01_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631412.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(26695..26853)
                     /locus_tag="NOA01_000175"
     CDS             complement(26695..26853)
                     /locus_tag="NOA01_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631413.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            complement(26994..27618)
                     /locus_tag="NOA01_000176"
                     /pseudo
     CDS             complement(26994..27618)
                     /locus_tag="NOA01_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(27615..27926)
                     /locus_tag="NOA01_000177"
     CDS             complement(27615..27926)
                     /locus_tag="NOA01_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631414.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(27970..28263)
                     /locus_tag="NOA01_000178"
     CDS             complement(27970..28263)
                     /locus_tag="NOA01_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631415.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            28457..29104
                     /locus_tag="NOA01_000179"
     CDS             28457..29104
                     /locus_tag="NOA01_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCI0631416.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            29165..30310
                     /locus_tag="NOA01_000180"
     CDS             29165..30310
                     /locus_tag="NOA01_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCI0631417.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(30391..30696)
                     /locus_tag="NOA01_000181"
     CDS             complement(30391..30696)
                     /locus_tag="NOA01_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HCI0631418.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(30721..31152)
                     /gene="ruvX"
                     /locus_tag="NOA01_000182"
     CDS             complement(30721..31152)
                     /gene="ruvX"
                     /locus_tag="NOA01_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCI0631419.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(31149..31415)
                     /locus_tag="NOA01_000183"
     CDS             complement(31149..31415)
                     /locus_tag="NOA01_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HCI0631420.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(31670..32395)
                     /locus_tag="NOA01_000184"
     CDS             complement(31670..32395)
                     /locus_tag="NOA01_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HCI0631421.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(32756..32826)
                     /locus_tag="NOA01_000185"
     tRNA            complement(32756..32826)
                     /locus_tag="NOA01_000185"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32792..32794),aa:Gly,seq:ccc)
     gene            32943..33695
                     /locus_tag="NOA01_000186"
     CDS             32943..33695
                     /locus_tag="NOA01_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCI0631422.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            33711..34190
                     /locus_tag="NOA01_000187"
     CDS             33711..34190
                     /locus_tag="NOA01_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCI0631423.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(34441..36021)
                     /locus_tag="NOA01_000188"
     CDS             complement(34441..36021)
                     /locus_tag="NOA01_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HCI0631424.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(36014..36757)
                     /locus_tag="NOA01_000189"
     CDS             complement(36014..36757)
                     /locus_tag="NOA01_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0631425.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(36933..37709)
                     /locus_tag="NOA01_000190"
     CDS             complement(36933..37709)
                     /locus_tag="NOA01_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCI0631426.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(37957..38793)
                     /locus_tag="NOA01_000191"
     CDS             complement(37957..38793)
                     /locus_tag="NOA01_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HCI0631427.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(38804..39733)
                     /locus_tag="NOA01_000192"
     CDS             complement(38804..39733)
                     /locus_tag="NOA01_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCI0631428.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            39925..40278
                     /locus_tag="NOA01_000193"
     CDS             39925..40278
                     /locus_tag="NOA01_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCI0631429.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(40352..41008)
                     /locus_tag="NOA01_000194"
     CDS             complement(40352..41008)
                     /locus_tag="NOA01_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCI0631430.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            41234..43168
                     /locus_tag="NOA01_000195"
     CDS             41234..43168
                     /locus_tag="NOA01_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCI0631431.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(43254..44477)
                     /locus_tag="NOA01_000196"
     CDS             complement(43254..44477)
                     /locus_tag="NOA01_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCI0631432.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(44471..46369)
                     /locus_tag="NOA01_000197"
     CDS             complement(44471..46369)
                     /locus_tag="NOA01_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HCI0631433.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(46363..47481)
                     /locus_tag="NOA01_000198"
     CDS             complement(46363..47481)
                     /locus_tag="NOA01_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCI0631434.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(47564..48397)
                     /locus_tag="NOA01_000199"
     CDS             complement(47564..48397)
                     /locus_tag="NOA01_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCI0631435.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(48390..49307)
                     /locus_tag="NOA01_000200"
     CDS             complement(48390..49307)
                     /locus_tag="NOA01_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCI0631436.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(49330..49509)
                     /locus_tag="NOA01_000201"
     CDS             complement(49330..49509)
                     /locus_tag="NOA01_000201"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631437.1"
                     /translation="MNKINENTSNTVLPATYSDATSYLTTAAYNYWQTGDFENSYLKE
                     AENFAHKYGVKLTGD"
     gene            complement(49534..50658)
                     /locus_tag="NOA01_000202"
     CDS             complement(49534..50658)
                     /locus_tag="NOA01_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCI0631438.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
BASE COUNT        15096 a        10237 c         8548 g        16840 t
ORIGIN      
        1 ttccgctatt tcattatatg aattttttat tctcgattga ttaatattta ttatatcccc
       61 acacaataaa agaccgtgat atttctcaca gtcttttatc tactctattg ctatttgctc
      121 tcttagctag tactttaaaa gctaactcat tgaggtagtg aaaacgagag gtgtgttcaa
      181 acagaaatcc aaaatcctca actgctagct gtggaatcgt agttttgtgt ttcacgatat
      241 ccgcaagcag gttcaaatct gataataaga attcttggct tatcagctgt ccgccaatta
      301 tttctaatgt atccggatgg tagatcagct ttgcatttac tgttttgttt tcttcatcgt
      361 attgtggaaa tagatcaaat tctcctgaac acgaaccggt attttgttcc aagaatggcg
      421 cttcatcttc tgtcagccct acagttccta aaaagtagtc tataatgact gctgctgtta
      481 tcttttgtct gaggtcgtaa gcggttggct gaccactcaa agttagcgct actgctcttg
      541 ccatatgtac agctcgagta actaaaggta aaaagagatg ccgtttattt ttgtcaaaac
      601 tgactgggac gatatctcca atcgcataga tgtccggatc acttgtctgc agatattcat
      661 tgactatcac agtctcatcc tcatacaaag ccactttttc ttttaacaat gtcgtattag
      721 gacgagtttg ggcagaaaaa atgaccatat ctgcctttat tgcttgagat agaagttgta
      781 cggatactac tttctcttct gattttttcc aatcgatcaa gaattcattt ggataaaaat
      841 tgatttgctt agtttgcagc tgattttgaa aactgttgat catatcctta tcgaaatatc
      901 ttgataaaac attcggcata taatcaacta gatgaataat gtatccttta tttctcaaag
      961 catctgctaa ttccaatcca atataaccag caccgaagat cactacttct tttgcttctt
     1021 ttaaagcggc aagtacttgc ttagattgca ctagattttt ataggtgtag acattttcag
     1081 cttcaatttg tttatacaag gtgacaggag aggaacctgt tgctacgatt aatttatcat
     1141 aggttatggt ctcgtttttt ccttctttgg atgcgaggat gagctgatgt ttgtctggat
     1201 caagttcagt tactctccat tcggtgatca gttttgcccc actttcttga agagtccgca
     1261 tgttatgggc aacttcagct aaactacgta tctttccttt taaataaagg ttgattccat
     1321 ttgaaacata gcctagtcca tctttatgat cttcatcgat cagtaaaact tctgctttcg
     1381 gttgaagttt tcttagattt agggcagctg tgatgcctgc atgggaagca ccaatgatca
     1441 cgattttcat gttttattca tccttaaatt caatttttct aaataacatt cttttaaaaa
     1501 ttggttcaaa taggggaaat cataattata ttcgttgcta gtgaatacaa aaactttagc
     1561 ggacttctgt gacgggtact ctttcaattg gctagtcaaa atgatatcat actgcttttc
     1621 tttctcgtat tcgactaaat catatttgat cgtattcagc aattgttcct ggatgatttc
     1681 tttcacgata tccgccatta gcctttcata aggaagatca taagcaattt gcagacgtaa
     1741 ttctaactgc ttgatcagcg tccgcaaaat agatgcatag cgagcaaata attcaaagcg
     1801 ggcattttcc tcgtctactt cagtaaaatc aacaatagca aatgtttgtc tgaccaatct
     1861 ttcacataaa accaggaatt gtttatcttc tacttcagtt aaccgctgtt ttaatccttc
     1921 ttggccaaaa atattcatcc atcctctaaa ataggcaaaa tgagcgtgat tttgcatcac
     1981 tgtcaacgca atcttttgcg aaatctcttc tggaaaaaca taaaaagaga aatattcttc
     2041 tatcgattgt ttaaacgttc gattcaattg agctactacg tcgtctttgt atttttcgct
     2101 tatcaaaaac tcctggacat gcgagttatg tgtatctaat gtaaagttac tgatcatgaa
     2161 aagataaaaa accaacgttt cattttgatt ccaaggaaag gcatattgaa agaagtaacg
     2221 ggtcaaaaat gtatggaggt ccaataaaaa ttgtgtcgtt tgaaacaatc caacaggaag
     2281 attgctataa tctggtttgg attttttcgt acagcgcatt cgcttcttca taatggaaag
     2341 ccatatttca aatttcaatc gtccttcttc tgtaatagta aaactaaact ctttttgcag
     2401 cagatctaaa aaagtgtttc ctgataaatc actgtattct tttttgtttt caagataagg
     2461 acgtccaaac caaaataatt ggaaataaaa ataacaaatt tgactttctg gaccggtcag
     2521 ccgcccgctt tttattttca gatgaaactc atttaaagaa tcatttaatt ccttcactct
     2581 gcgagacaaa gtagccgaac tgatattaag ttccatgaca agtttctgcc gatcaaatga
     2641 gccattttcc aacagataaa gaagtatctg atattttgta gaatagcgca agtagcaatg
     2701 ataataaaca tcgactgctg ctatatttcc atgtctgatt aataaaatat tcccttcttg
     2761 gataacaatt tccaattgtt tgatattttc caagttagct tgtaaataaa acagatcttc
     2821 tttcaaggta gcccgatcaa ctgataagcg ggtaagtacg ttattaacgg aaatctctcc
     2881 tttttcctct aataaaagtt ggaaaagttt gatttgccgt tgttcatttt tttcaagcaa
     2941 actgaaaagt tccaagtcca ctcctccaag catttctgat tttaacagca gaagtttcct
     3001 tctcatcctt gaaaaagagg atctgtgaca gttatcacag atccatacaa attctaatca
     3061 cttagctggt tggcccagct gtcagttccc atgtgaccat gccttgatac gttgcgccag
     3121 gttgagcatt cgtactgcta tttacctcta agagtaaacc atcggtatcg ggaaagtcaa
     3181 cgagaacctc tcctttagct gttccatcac tctcataaat caacgtgttt tgaccttgct
     3241 gaatcaattg ttttgatcca gaatgattga agtataattt tgttccgctt aattcttgac
     3301 cttgatcatt cttaaataaa gaagtcaatg tccctgtcag ttgccaagga gacgtgcttg
     3361 gaagccgagt atcccgcaca tcaaaagaaa aatcgccttt tgcttcataa taacttgtct
     3421 tcaacggttt ggggagatcg ttaccaaatg taaaatcatc tggtacatta actaatgtta
     3481 attcttgttt ggggacaaga attgttttag cagatgtttg gacaactggg ttaggaagct
     3541 tagcaccatc tgctaatgta atattgctgg aaagtacagc tgcctttgtc agtatgctga
     3601 cggattgtac agaagtatct acagtattta attgatagtt gtaagtaaga tgaatggtat
     3661 ctcctatttt gctcaataga tctgctggga cagaaactcc ttgttgaatg ccggtatctg
     3721 cgatagttgt accatttaat tttaaggaac cattgatata agtagtattt tcaggaagtg
     3781 agatcgtgat ttttttgtcg gttatcgcat ccccagattt tgttaacgtg aatgtcacat
     3841 tttcttgaat cgtatctcct gatagagcag acaggggatc tcctgtctca ggttgtctgc
     3901 taaggttttt tagctgacta tctgcactca cagatggaaa attagccact gttatttgag
     3961 gaagagtggt gattccagct tgttcagact gccatttgta cgcaaaagac ggatacatgt
     4021 tagtaagtac atcgtccacc aagcgataat caaaataata ttgccctgaa gccaatgcag
     4081 gtagtgtaga agtagctgta tttacacccc cgttatttga ttggatcgtt tggaaaagtt
     4141 gatctgtcca tcctgtcgct aatttttagg ttcctttcga ataaagcctg taatggatcg
     4201 aacccttttt tagttcagtt gctgaccaat tggccgtgac ttggtacgat gcagaagaaa
     4261 ggagctttcc ccactgacta tcggctgtta agtttgttgg taaaaccgtt ccgtctagct
     4321 tgtactgctc caataccttt aaagcaacag gagaaagcaa tccgccttgc gcatctttcc
     4381 cactgtacac gatattggct gtctgattca atccacttgt gcttgctgga gtatgagtca
     4441 gtattgctcc ttgtgtaacg ttttttcctg taaaatagtt gactacgcga tcaaagcgac
     4501 taaatggcaa ctgatcaaaa cgtccttcag tcagccccat cgtcaatgtc cgactattgg
     4561 tagagcctgg aaggttctgg ttatttccat ccacgtattc tgtggtatca gcaaatgtat
     4621 tatattttcc tgttgaatca cttgttgaga tgtaactatt ccacgtattt atctctgcac
     4681 ttgcagaaaa tgcatatgct cctggattca agtttccgcc agttaattta ggataaaatc
     4741 tacctgcagc tggagctaaa atcgaattga acatacgatc ttggataata ttattcgaat
     4801 cataaaaatc aacagaaaaa acggcctttt tagacataga aggttgtaat ttacccttgg
     4861 tcaaaatttt gtttggactc gtatcatatt tatctccgtt ttctgtcatc ttcgcgacat
     4921 tagctgtaac atcaaattga agcgggatat cgtccacgtt gaattcgcct cgcgtaataa
     4981 tagcaataat atctgccaat agagtatttg tatcccacga ccccatccca aaacgcaagg
     5041 tatggcttcc tgctgataat aaactaccgc tagtaacctt gatgggggca ccggcaatat
     5101 acaagtaggc gttgttccag tcaattgctt ttaatacatc actgcttgat acatgttccg
     5161 gcaaattgat gatcgccaca aatgcaaatc cttgtgcagt gatattcacc aaatcaagta
     5221 catttgttag tttcacatgg ctttcccaat gaaaacggac cgtgccacct ttccaaggat
     5281 catttgaacc atcgttcaaa taagtagcag tcaatttagg caattggtta gccccagtta
     5341 gttggacaag cttcccgcca ttgactgcac tacgtccacc atttggaggt acgatatttt
     5401 catacgtgta tggatcataa gaaatggcag gaggcgttgc tccaacagct tctgccgaag
     5461 gtgaaaagat aaatatactt aaaacaaaaa gcgaagtaaa aaagaaggag cgaaaaaatg
     5521 aaaatttatt ttgtagcaac atgttcatcc tccttttcct ttcttctcca taacaacaac
     5581 cacgcaagaa gccataattc tataccaatg atcaaataaa gaaaacggat cttttcttca
     5641 ttcgtctccg ggaatacgcc atgttgatta gattctttat aaatcggtag ctgtgtctgc
     5701 tgttttgatg cgccattgac tgaaccactt ggatgcggaa ctggacttag atcataaaat
     5761 gtaatcgtac ctgaactttt ggtatctgct tggactggct cttgccagct gaagaacagc
     5821 aaacttgcaa taatccctcc caataagatc gatgttttca acgaaatcac ctcatcatgt
     5881 tgtttctcct tttttctttt tcttgtggac ccacaaaaac agcaaaatca accataacac
     5941 tagaactgca gcaaccaatc ctgcataaag ccatgggaaa gattgttctt gtactgcaaa
     6001 aacggaattt tcatttactt tttgtgcttt ttcttttttg atcgtaaagt tttgtgtaaa
     6061 tcgccattca ttactgtcgc ctgattttgc tatgatttct gcgcggtaat cgccggcttt
     6121 tgctttatca ccagctaaat caagattcaa gtgaaaattc gaatttggtg caactaaaaa
     6181 ttcattagac gtattttcaa tataaagctg attttcataa aagatcttgc tcgtaaatat
     6241 caaattgctg ataatcctag gctgaggatt ttggaaacga agctgaacag ttggctttgc
     6301 gttgacctcg tccaaatctc ctgataaaag tttcagatcc ggttctatcg tctcttgact
     6361 ttcttgtaaa acgactgcga gattataagc aatacgattt ttgatttgtg ctccttctgt
     6421 agcattttct gtttcagctt cttctttagg tgaaacatga atacccgcta atacacgtcc
     6481 cttaaagctt tttgctggga ttttcacttc tataggtata gttgtttctg catgtgctga
     6541 tacattgatt tcctttttcg caatgagcaa aacgtcacgg acatcaaacg gcaatgtctc
     6601 atctaacgtt gggttttttt gactataatc gatcgttcca ccatcactcg ttaccgccgg
     6661 attgacagag acctcaaatg ttcgttcttc atctgaagaa ttgctgataa ttacttctag
     6721 tgtctgattt tgttccggtg ccactaataa atcaaaataa ccgattgctt tatctatttg
     6781 gttctctgga aataccggtt tgattccaaa gtctcctgct gaatcctttg cttggctgat
     6841 tgacggaaaa atgaaaaata gacttaaaat agaaaaaaac gaaacaaaca gtcgtttact
     6901 ttgattcatt gtccaccaca tcctttcaac aacaactgcg gtaaaaaatc cttcacctat
     6961 agtattttca ccgcagtttg ttgaatttat ttcatactct atgctactgg gccagccacg
     7021 aggttccaag tcaaatcagc cgtgaatgtt ccagctgctg ctttcggcac tttcagtgtg
     7081 atatttgctg gatcataaac agaaagccat gttcccagac cttgatcttt tgctgctgta
     7141 aatattggtg tcgctgcact tgctttactc aacccagtta cagtttgact agttggtcca
     7201 gtggattcgt tatctgacgt acttttaaca gtggatgttc caaaatctaa ttcagcgcct
     7261 tgcaaaactt gtgaaccgtt tacaaaatct gaaaccgaaa ctgtcacgtt ccacccttgt
     7321 gcttggccat ctgcacctac acctcgacga tctgatactt ggagataagg tgtatcgttt
     7381 tttgaagcgt ctacttgata cgtttttgtt ccgctttcaa tctcgtgtgt accaaatggc
     7441 aattcaggaa ctacatccaa tgttaaagaa cctgtctggc cagttgctgg attttccgga
     7501 tctgctggct ctactggttt tgaaggatca gtagggttaa gaggctcaac ggtatttgtt
     7561 ggttcagtaa aggtaatatc tccttttgaa gtcgcttgac cctcagcaaa agctggtgtt
     7621 gccaagccgc ctagaagaag tgttgaaagt aataatgtcc caatcatgtt ctttttcata
     7681 ataaatcact cctttttctt agtgacctaa gtatagtaga gacagtatgc aaaaaatcct
     7741 catgaattca tccgcttact tgaaattatg accctttttg tataaaagag aaaaaaacaa
     7801 taaaatcgtt tacatgaata ttcttccttt tttattaagt atgaaaaaat atttcaattt
     7861 tattttttcg tttcaaatta ttctattagt gacatccttg aactataaaa ataggagtac
     7921 ggcatcatct gccgcactcc tatttttttc gtcatttatt tcttaaataa aacgattctc
     7981 acgcaatttt atttttttga tatcttctac tgtttgtgtt ccagctaatt gcatgaccat
     8041 ttccaattct gttttaaaga aatcaaacac ttgatggact cctgtacttc ctcctaaaga
     8101 caatccataa ataacgggtc tgccaatagc aacaagatcg gcacctgatg cgattgcttt
     8161 gaaaacatgt tgtccacgtc gtacaccgct gtcaaacacg attggtacac gtttatctac
     8221 tgcttctgcc acatattgca acgaatcaaa cgcagcaggc cccccatcta attgacggcc
     8281 accatggtta gatacccaaa ttccaccagc accagattct aatgatcgat agacatcttc
     8341 ttctgattga acacctttca cgtagactgg caaatcagaa tgagcagcga taaattctac
     8401 atcttttgga ctaagttttt gtttggaaga tttatataca gcatccatcg tttggcctac
     8461 tcctgattga taagcctgaa cgatcggcat tggcagcggg aaggtaaacc cgtttctgcg
     8521 gtctgtttca cggtttccgc caactgttgc atccgctgtc aatacgattg cttttgctcc
     8581 attacgcttg gccacttcaa gaatatccaa attgattcca ttatctttgc tcatataaaa
     8641 ttgaaaccat tgcggtgctt tctccccgcc tgcttctctt atctcttcta aagtacaaga
     8701 agcgtaagaa cttgctgtat agatcgtccc aaaatctgct acaccttttg cagaagcttt
     8761 ctccgccttc acatgagcta atccatgagc agcaactggt gccatgatga ttggtgcagt
     8821 cagcatttct tcgtcaaaat gtgtagttgt atcgggtaat tctacatctc ttaatacatg
     8881 aggaataatc aaacgatgat tgaatgcccg ttcattttct tgataagtaa ataagtcgcc
     8941 agcaccactg ctgatgtaac cataaccacc agctggaatc acttgtgaag ccgcgatttc
     9001 taaatcttcc atattgataa aatctactat tccttcattt gtacctgctt gatatgtttt
     9061 ttccatctca atgccttctc tctataaaat ttgaatgatg aacaatatga tagcgtttac
     9121 actttttata ttactcttcg acattgatac aggcaaataa tctgtttaca tttcagccat
     9181 tccagatgtc actactctgg aagaaatgat tgtaataaaa atagataaaa aaccgatggg
     9241 actttgacaa aagtagtgtc aaagtccctt tttttgaata aacggtgttc aaaagccatt
     9301 ttctcggtat taagtcaaat ttatgcaaaa atgtggaaag ccattttcac aaaatttttc
     9361 ttactactcg aaactaagcg actttttcac aacctctcga taaaacggtg ttcaatcagc
     9421 tgaccctcgg tattagctca aaaaatggca aaatatgagg aactattttg ccatttgtct
     9481 ctctaatact caggtcagaa cgttgacttc acagtctctt ccttaaacgg tgttcaaaaa
     9541 cttgcatctg cggtaataag cccaaccaaa cgaaaaatag gaggagctat ttttgtttgg
     9601 ctgttcttat tacttgatgc agggcaggtt tttcacgacc tctgattcac ggtgtttctt
     9661 tttcatcaac atttttacga aatactgtgg aagactattg aaataagtgt ggattgtttc
     9721 tttgtgtttc aatcgtttct tgccattctt catttaattt tggattaccg ttttcttcct
     9781 tgaactcata aggtcctaat tcaaaaatcc cagacagtat ttgattcacg atttcatata
     9841 gttcagtgtg atcagacaca tgattcttga agatcatctc attatcacca cgtttgaacc
     9901 tcacattcgg gctctcatga tgaacctcta tgcatttttc tccttttttt aactgacgga
     9961 cttctgaaaa catctcataa atatcttctc cagtaaaacc ttcagcaagc gccagactgt
    10021 ccacaagacc gcctactgtc agttcagaga ttattgcttg attggaatct aattttaatg
    10081 tatagcttgt cgcttctact acgccacatg cattgcaacc gccttctgat agttcgcttt
    10141 ttctaatcaa ttgaacttct ttcattttct cttcctcctc ttaattttct acttatgtat
    10201 ttttatcacc tgacataggt gactcctatc aacgctatca tgtgcttttt tcctcagaaa
    10261 tcctatttat ccattccatc tatcccttat tcttctactt ttctttctct agcatataag
    10321 agatacgcat agtattcaag caaggattgt cgatataaaa agaacaaata ctgctatttt
    10381 gatttctttt ggctatatta catataaata aaatttatca tacctgctct ttttgactat
    10441 atcatcatct gacttctata cttcgtttgt attaaaactt aggaggaatc attcatgctt
    10501 acagggttaa acttattaaa taatcctttt ctaaataaag gaacagcttt tacaaaagaa
    10561 gaacgggcaa aatatggaat cactggtatg cttccaagta ctgtccaaac actggaacaa
    10621 caatctgttc aagcttatgg tcaatactta agcaaacaaa cagatttgga aaaacgtatc
    10681 ttcttaatga atctattcaa tacgaatcgg acattgtttt ataaattgat gggcgaacat
    10741 ttagttgaat tcatgcctgt cgtttatgat cctgtcgttg cggattcaat cgaacaatac
    10801 aacgaaattt tcttgaaacc gcaagatgct gcatttcttt caatcgacga accagaaaac
    10861 atcaaagcaa gtttgaagaa cgcggcagat ggacgagaca ttcgtttgat tgttgtgact
    10921 gatgcagaag gaatcttagg tatgggcgac tggggtgtga acggtgttga catcgctatc
    10981 ggcaaattga tggtctatac tgctgctgct ggtattaatc cagcacaagt tttgcctgta
    11041 tcgatcgacg ctggtactaa taatgaagaa ttattgaata atcccttata cttaggcaat
    11101 cgtcatgctc gtgtagaagg cgaaacttac tatgaattca tcgatcaatt cgttcaatca
    11161 gctaccgaat tattcccaga attgttattg cattgggaag attttggacg aggaaatgct
    11221 gcagcgatct tagaaaaata cgaagatcaa atcactactt tcaacgacga tatccaaggt
    11281 acaggaattg ttgtcctagc aggtgtatta ggcggattga atatatccgg tgaacaattg
    11341 aaagatcaaa taattttgac atttggtgct ggtactgctg gtgtgggtat cgcaaacatc
    11401 cttttagatg agatgatccg tcaaggtgta cctgaagaag aagcacgcca acatttctat
    11461 caagtagata aacaaggttt attgtttgaa gatacagaag gattgactcc tggtcaaatc
    11521 ccattcgctc gcaaacgctc agatttcact aatgctgatg aattgacaaa cttagaagca
    11581 gttgtaaaag cgatccatcc gacaatcatg attggtactt ctacccaacc tggagcattc
    11641 actgaagaaa tcgtgaaaga aatggctgct catacaccac gtccgatcat catgcctttg
    11701 tctaatccaa caaaattggc agaagcaaaa gcaaaagatt taatagaatg gacggacgga
    11761 aaagcattag ttggaacagg tatccctgct gctgatgttg aatataatgg tgtaacttac
    11821 caaatcggtc aagcaaataa tgctttgatg tacccaggtt taggacttgg tttgattgct
    11881 tcaacagcta cacgagtaaa tgctgaaatc atttctcaag ctagtcgagc attaggcgga
    11941 atcgttgatg taacaaaacc aggagcagct atcttgcctc cagtagcaaa aatcactgaa
    12001 ttttctcaaa cgattgctga aactgtcgca aaatcagttg tcgcacaaaa cttgaatcgt
    12061 gaagaaatca cggatatcaa agaagcagtt gaatctgcta aatgggttcc tgaatataaa
    12121 tctttagaag attaagatcg ttatagtaaa agaacataca gaaactttgt atgacgtcta
    12181 aaaagaaaaa agcgactgcg gcaaaacagc ggccagccaa ctcctgctct tttgtcgctt
    12241 ttctttggct tttttcatat gatacagaat aagaaaggat ctgtaaaaat ggcacaaaaa
    12301 acaacaactg caactgcagt tgaggaaaag aaaaaagaag gtttttggtc cacaaaaatc
    12361 accggcgtaa gtttgccact ttatttgatc atggtccttg tcctgatcat tgcaatggct
    12421 ttagggaaat taccgacgaa tatgattgga ccaatcgcag ttttggttat tttcggtaat
    12481 ttatttcatt ttatcgggac aaaaatccct atcgtcaaaa gttatttggg tggtggttcc
    12541 gtttttgcga tcttcgcttc tgctgcgatt gctacatttg ggatcttacc tgaatatgta
    12601 gtgaaatcca cagaaaactt tgtcagcaat atgggctttt tggactttta tattgctgcc
    12661 ttgattactg gtagcatcct tggtatgaac cgtaatctat tgatgaaagc ttctgttcgt
    12721 tttattcccg ttgcacttat ttcaatggtc gtctcctttt ttgccgtagg actcgtgggc
    12781 atgcttatcg gaaatggttt tgcaaattcc gttttataca tctcattacc tgcgatggct
    12841 ggtggtgttg gagccggagc cgttccttta tcaacgattt atgcaaatgt tttaggtacg
    12901 gatgcttctt ctatcatttc ccgcctgatt cctgcgacag caatgacaaa tgtcttagct
    12961 atcatcggtg cagcattagt tgctcgtgct ggacaatcaa tgccaaaatt caacggcaat
    13021 ggtaaattaa tggaaaaagg cgatcttggc gatggaaaac ctcgaactgt gaaaccttcc
    13081 attccacagt taggtgttgg tttgatggta tcattgactt tcttcatctt aggacaaatt
    13141 ggtaactatt ttgttcctaa agtccatgct tacgcattta tgattattat cgttgtcatc
    13201 tgtaaaatcg ccaacctatt accagagtat tatgaagatg cagctatcat gttcaataac
    13261 ttgattgtca aaaacttgac agcagctgtt cttgccggta tcggtattgc gttgctgaat
    13321 ctgaatgtat tagcttctgc tttgacatgg caatttgttg tcttatgtct gacaagtgtt
    13381 atcgcgattt cagttgtttc aggatttgtc ggtcgtttgt ttggtttata tccaattgaa
    13441 tcaactatta ctgctggctt atgcaacaac tcgatgggcg gaacaggaaa tgtagccgtc
    13501 ttatctgcag ctaatcggat ggaacttatt gcttttgctc aaatgggcaa tcgacttggt
    13561 ggtgccatcg ttttgatcat ctctggtttc ttgatgcaat tactttctta gtcaaaaaaa
    13621 caaaaatacc tgatcatcaa gtatcggctt ggtgatcggg tatttttttg ataaagattt
    13681 acaggatttt caaaaatctt ttttgcattt tatcggaatg ataaccgatg tgttcataaa
    13741 attggtgtgc ttctactctg tattctgcag aattaagcct tattgtcgtt attcctcgtt
    13801 ttttcgcttc ttcttctacg ccagtcatca aagcctttcc gatgcctctt ttttcagcat
    13861 atttagacac agcaagtgcc ataatattga acattggatc gctaaaaatc gattcatagg
    13921 actccgcttg tacatagcca gctgcttttt tagaagtagg atcttcataa accaagaaaa
    13981 aatttttctc tggctccttc agcagtttct tcatttgtct caccgtaacg gttaatggta
    14041 tatcataccc taattgatcc gcattgattt cacgtagtgc ttctgcatct tctaaacgca
    14101 tttcccgtaa catccaaatc accagccaat ttctgatttt ttaaaatgaa tttttttatc
    14161 atagtatact ctttttttca tttagaacaa tatgtttcag tgattttgac ttgcgttagt
    14221 ctagattctc ctaatgtttg taagatgaca aaggcattgt aacaattgac caaaagaacc
    14281 acgaggacaa ttacttgaaa aataaaggtc actttttttg gagacaaaac cctgctaaat
    14341 cttgctcccc acaaacctcc aataacagca gcggggataa cataaaacaa catagataaa
    14401 tcaaagcgca taaagctact tgttaaagca atcgttacca attttgccag ttgagaaaag
    14461 aaaatcgtag atagagaata tagtgttgct tcttttattg gcaatgcaaa catcagcatc
    14521 aacaaagaaa cattgactgg tccaccacca atacctaaaa aactagctaa aaagcccaga
    14581 acccccccac atataaggta ccaagtccat gacgtcaaat gaaattttgg ctgatgatgc
    14641 ttggtgtaga agaacgcaaa aaccaaggtc aataccgtca aaaaaatttg gatcagctgc
    14701 acatgcttct cgttttcaaa taactgtaaa aaaacttcga atacaatgtt gccgactact
    14761 cctccaacaa cagctccact agatacccaa ccgacaatct gccaattcag tgatttcccg
    14821 cttgatactt gtcgcacagt cgaaacaatg gacatcgtga ataccgcaac tgttgagtaa
    14881 aaagaaatcc ctgcaacaga atgggcgccg ataagatcaa gaatcggttt gatcaacacg
    14941 cctccaccca tcccggaaat agcgccaact gtgttcgcca gaacgatcac cacaaaataa
    15001 atcagaccaa tcatcttgta tttcctttct cttgtttata tcttttagcg attgttactc
    15061 ttttctttta ttatacagta aaagaaacaa acgaggcttc gaaagctttt ctacttttta
    15121 aggaaattta gtgctaaacg aaaagaggtt gtgaaaaagc tgtcctgcat caagtaataa
    15181 gaacagccaa acaaaaatag ctcctcataa ttttcgtttg gttgaactta ttaccgcaga
    15241 tgcaagcttt tgaacaccgt ttaagaaaga ggtcgtgagc ctgccgttca gctccgagtc
    15301 acaaggaaca atttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg
    15361 aggagttggc aggagaacac cgtttaagga agagactgtg aagtcaacgt tttgacctga
    15421 ttattagaga aacaaatggc aaaatagttc ttcatatttc gccgtttttc gagcgaatgc
    15481 cgaggggcag ctgattgaac atcgtttatt tggaaaaagg gattgtgaca agacgtttgt
    15541 cacaatccct ttttttatat cttattaatt cattcttttg ttctattctt cttcttccgc
    15601 catgaatgag aaacccatat caaagaacta taaaggttat caaaacagcg tttatgacta
    15661 aatgaattag agttatctat cactattttt caagcgattt aaaccaaatg gcatacaata
    15721 tggcatacaa aaacttcaat aagccgtggg ggtttttaaa tttcttcttg ttctttaaaa
    15781 tcaatgagtg gagctacata atttgaatcc actttttcaa acgtcacttt ttgcatcgac
    15841 gaatgttttc tttcccaaga taacgtaaat agatttgact ctggacttat aaagtctact
    15901 tcaccttcta gtattacttt cttatgcaaa tattcctcta ataaatatag acttgctaaa
    15961 gcatacaaca cattttccaa atttgcttcc ttataatttg aagaacgacc atgttttact
    16021 ctgttatata ttttccacca atctggtgta tcttcaggcc acccatctcc aaaaggtttt
    16081 agtgaagtca atgttaaaga attcataacc ctaacaccat tatccaaaca cattaattcg
    16141 ctagatttaa taacagcttt aaaatcagtt atgcttttat tgctactgct atcaaatccg
    16201 caaagttctt tgaaaataat ctcacactcc gaaccaatcg ctagtagtag tttttgatat
    16261 tcaatagaga atgttttgaa attattctca tcgagcatca caaaatctgt ggtattaata
    16321 aactcatttt ctaaatgtag ataatacttc caataaaaat ttgtaaactc tgctttgtcc
    16381 attaaatcac ctcttttgaa ttataacaaa aaaaacacgt ccacaacaga cgtgttcttt
    16441 gatttctaca caatcagctt gccaaaacca tccaatcagt cctagcatgc ctgtattaaa
    16501 attgaacgtt catcatctat catctttctt agtccagcat gtttgcaacc acacctacaa
    16561 ttttcagtcc aacaatagat ttcgactagc taagtcttgc aaaattcagg cagctaaccc
    16621 ttcatttact accttaatta tactaaatac ccacaacgtt tacaagtgta atcattctaa
    16681 gaaatctgtt tagctatgat cgttgtataa tctttaaacg ccgttccttc attgattgaa
    16741 ataattttat aactcttgtt tttccaagct actttcatat cgtttgttag ttcataatct
    16801 tgatcgtatc gaataataaa cgtcaacgtt ccttcaagaa tggtacctac actagcaagc
    16861 acgtctttta gcatttgtga acgcacatta gcccaacatt caaatacagt ttcttcacta
    16921 tccaccatct gcccatcttc atcttttacc cgttttgtct ttttaaactt gactctctca
    16981 gtcaattcat ttacattctt aatgattggc ataataagca cctctcattt gttgtactaa
    17041 tgcttgtacc gttactggta tccgttcgct gctggcttct tgacggttca aataccaatg
    17101 ttgagtaagt aatgacaccg ccaaatcaaa ttgctgataa ttatccatat ctttatccgt
    17161 tgcgctacta tcaatagcac tttttatata ttcgcctgct gtatcaatca attgttgaat
    17221 aaagtcatca tcaatagtgt gatcgatccg catacttttt tttatgctat ccaaaattac
    17281 cattttatct ctcctaatga aaaaaaggca gtgggataaa ccgccgccca atatttttct
    17341 atacttttgg tgttacaact ggcgtaaact caatatacac tgaagcattt tgatcaattt
    17401 tcttataatc gttgcgaacg atcactgcaa gtccttgtga gtagtaatcg aatttttccc
    17461 attgagtcgt tacttgattt ctacgagcaa caaagataga ttgtgcaata tcccccataa
    17521 tcatagggaa tgttccagct ttaggattgg caaacaatgt atctgcaatc aatactactg
    17581 gcataccgaa taatgattta ccactaggtg ctgtcacatc gggttgtaaa atgtaacgtc
    17641 catctgaatc tttcaatgta tctaggtggt aatatccgct ttggtttaga attaccattt
    17701 tatttaatgc tgggtctaat gccacattgt atagttgttt caaatcgtcc aacgtagcag
    17761 ccgttttctt agtgaatgtt tttaataaat ccatgatatg cttattgtca gtgttttcta
    17821 ccaatttcgc taactgatct ttgacctctt gtacaatatt cactgctgaa tcttccacaa
    17881 cctcatttga taaggcaatc ttaccagcac gagtttctac tttataatca actgaagtaa
    17941 acatttcagc gtcaatatca ccaatttcag caagttcagc ttttgttgct aacactgctt
    18001 gttggttagt tgctactgga tatttacctt gtccatttga tactgttttt actgttgcat
    18061 attgagctaa gttataattt gaacgtttca aatcaaagac ttcaccgatt acttcttcgg
    18121 gaacaactgc cgctgcattt acagtagtga ctccatcacg cacttcgcct tgtgaacgga
    18181 tatattcttc atacccacgc aattctgttt tttcgttatc gattaaagtt ttttccatgt
    18241 tattactctc cttttgtgct ttagaagtgg attctaagct attgtttgtt tgttttgctt
    18301 ggttgttact cataaacgat tcgtaagaac gcttatttac ttggacattt gatgaatcat
    18361 aagcgggaac ggtcacgaca ctaatttcat taagtgcttt gattttatct attgatcgtt
    18421 caattgtgcc atcttctttt ttgtcgaatg aatctaatcc aagcacgaac ccaaaggaca
    18481 tagaatcaac aacccctttt gagatattct catacacatc atttgaatag gtcgtgtcgg
    18541 ttagctctgc ttcaaaatgt aatcctgtat catcgacatt caatttcaac gtacctgctt
    18601 tgacactagc tagtggcttt gaataatcgt gtccatataa taagaacaca tttgataaat
    18661 ccacctcttt taatgcttcg ggtgtaatca cttctacaaa gccgcctaaa tcttttgacg
    18721 gttgcccgaa tttcaacgca tagcctgaaa tagttttgcc gtctgttggt tgtttttcat
    18781 tttctttctc tactttggga gaatcggctg ttagctcagc ttcttctgtc agccgttttt
    18841 ccttttcttc ttcattattc aactggtgta actccttcct gttcttttaa ttgatatttc
    18901 tccaacgtat ctaaatacgt aaagttcaaa cttgctagta aacgatcgcc accatccatg
    18961 gggggtagcc ccattttcga ccgcccctca ttaatcgtga gaagtgaccc tttgacctga
    19021 tccaatacat tttttacttt tgtttcagga tcagtctcta gtaactgatc ggaattaaag
    19081 cggatattgg tttcaagttt cgtatctaat tcactcacaa acacattaaa gtaatgaatc
    19141 agcgtacttt ggatatactg gagattactt tgaacggtgc tagaatgttc attttcaacg
    19201 cctaaacgct caatcggtac accaaacgcc ttggcaattt gttttgtgtt ccaatcattg
    19261 gaattgataa gcttcaaaac atcggtgttt atttctaatg ttttgtagtc cattgtttca
    19321 tcaagaatga tcgttctaag agcattatct ccactacttg aaccgttggc ttcttcaaac
    19381 ttttcacgaa ttgcgttctt tgctgatccg tctaaatctg acttatgaac cttcaaaata
    19441 cctgacccac tgacaccacg agtaaagaaa ttgtgcaatg tgcggttgcc agcttgttga
    19501 attttcagtt catcacgtaa cgcataaagt ggtggtaatc ctgttaaacc atcttgggtg
    19561 aaatatttga agtgcaaaat atcgctagac ttcacacgtc tttttttatc accaatctga
    19621 taagacaacg taccattatc taattgagta accgttacac ttgagttagg taataagtga
    19681 atttcttcta ccttttcacc gttgcgttta atctctgcaa agctattacc gtttagcaac
    19741 atattgaccg ccaaagcaaa ctttaaatgc caaccatcca tttctgaatt aggtttctcg
    19801 tttagtaact tcaccagttc attatcagct tgtggcatat tgtttttgac caattgaatc
    19861 ggacttgaag caatatcact agcaataatt cgaacggctg caaacacatc actatttctt
    19921 aatgcacgaa cgcttgtaaa gctggtggtg taatcatccg attggattga taccacatgt
    19981 tgtaggaaag gatctcccgt tgtattttgt ctcgctttaa aaaatgccat cttttaacct
    20041 cccttctgtt ctgtatttag catgaccgct accccaataa gcactatgcc cagcgctaac
    20101 aagccatagt agacatttgt tagtaaggtg atagcaacca acacacacac caatccaaga
    20161 agtaaaagaa cggtatgaat gtaattagaa agaaaattct tcacttgtat aaaattcatt
    20221 gtctgctttc gctccttccg ttgtggtgaa gtaatccatt gcaaacacat aggcattgat
    20281 taatgcagct attggatcaa ttttgttact gttctttgat ttattaatct gaatgccgtt
    20341 gttatccgtt tttaaaatag cgttgttcac tgcatgggtg agaatcgtat ttttattgtg
    20401 aataacgttg ccttcataaa cttgttctct aaaagttcgg gtcggaacgt ttagagtaat
    20461 cgttccttgt cttacttcta acattgggta gttggctttt tcagctttac tgataagcga
    20521 attagcgtta tacgggtcat aacaaatccc catacattcc aaatcatttt cttgaatcaa
    20581 atcttgaata aactgaaata cttggtcata gtccacgatt ccactttcta attgagtaat
    20641 ggaacactca ccagcttttt ctagttctcg ataaggcaaa ccgtcgcgct tttctttgtc
    20701 ttgaagccca tatttggtgg ctacaaagct atgtgaatca caataaagtt cgccgttatc
    20761 tagcggtacg atccatgaaa cgctagttaa atcatctgtt ttagataaat ccactccgat
    20821 ataaacgggc ttgcctgtaa tatcttgtgg ttctacttcg gttgcctgcc agtcttcagc
    20881 aatcatatag ctgtcctcac tcgcttgtct ccataaattg aagtttttca ccaatacaga
    20941 attaaggtta ttttgtttca aagcaaggtt cacatcatct tgaatggttg gaatcattac
    21001 ttttttgatt tcttcacttt caaaaatcgg attggctttg atccatttct cttgatcgtg
    21061 aatttcttcg ggatcgtcca attcccatat cgctataaag tacctgtcgg cttgttcttc
    21121 gccttttagc acacgatcta acaataagta ttcctcgtac ataggaacgt ttaaatcaag
    21181 cccagcggta ctaataacac acaataagcc gttcttttgt tgggtcattc ctgacttaat
    21241 gacgttgtac gttttacgag tttttgcttc gtgccactcg tcaagaattg cggttgttgg
    21301 tgcaaaccca tctagcgtgc tggtttcact agccagtgcc atagcgaacg aattagaggg
    21361 caagtgctga atcttagagt tcataatttt cagttgtggt cttaaaaact tactagactt
    21421 gaccacgttt ctaagtgaat tagacagcat atcatagccc aatttagctt gtttcaaagc
    21481 gttacttaca aacaatactt gccttgcttc tgctggttct tgttccataa tcaacgcatt
    21541 ggcagccatg ccacttgcga tatacgtttt cccattctta cggctcatac tggtaaacgc
    21601 tcgattgaat cgcctgtaat tcccttcttt agtacgccag ccataaagac tgcctagaat
    21661 gaatttttga aatcctaaca tcttgattgc tttaccgtcc gtagatggta gcaactcaat
    21721 gaatttaatc gctttggtgg cttgttcttc atcgaacaca taagggaagc tatctgactt
    21781 agaacgctcc aaatcgctta tatgacgctc acacgcttgg ataattttct tgggtgcaat
    21841 cagcttacct gataggactt tctcaatgta atcattcatg aattcatcaa ctctttgaac
    21901 gggtcttcgg gttcatcatt tttaaccttg ttcaaagcta gttttgatcg tgattctaag
    21961 gtcataccta gtgaggtggc ttgttttttc aaatcactca tagcttgtga ttgaacccga
    22021 accgctggat tggctttctt tactccgttt tcgcatgtga taaacgtacc tgattttctt
    22081 atctcggctt cggctgtttt cattcgagca aaggctaaac aataggctac caatgaacca
    22141 taatctgctt cactcaatgg ataatctttt tttaatgttg gtacgatacg attccattca
    22201 cttctagcgc tcgctgccat ccagtcaggg ggcgtgatat ttattaatgg ttgctgcttg
    22261 aacaattctt ctctagcgtc cacacggatt gctttttctt cacttgatat attgcctttt
    22321 gtatcgtgta atattttagg gtgtcccatg ttgtcctcct ttcagacaaa agaaaaagga
    22381 acgtaagcaa ttaagctcac attccttcgt ttttcgatca gaatttttct ttatctaatt
    22441 tatctatata aatgattttt ccgttctgca tttgaatggt cacttgacca aaatgaggta
    22501 actcaatggt gcgctgtttc cctgttttat cagtaataac aattgtattt ttcaataaat
    22561 ctcctacttt cattatatac tttttcactt gtgattacaa gtgtaatcgt tatttgtaat
    22621 tacccttatg ccttttcaca acggttattt gtaccaaaat gagtaggcat cgattgtcag
    22681 taagtttcac actatgcccc cttaactcaa agggtactct tttctcgtct ttgtctttca
    22741 ttggctgtct tggaattatg gtgtgcatgg cataaagaac gaaaattagt ttcatctaac
    22801 cgtctattcc aatcatcttt tagttcaatt ttgtgatcta caacatcagc tttccttatc
    22861 aagccttctt ctaggcaatc ctcacatact ggatgattta accgatacaa gtatgaagcc
    22921 tttcgccatg ttctactttg atagaattga aaatatttac cttcgctttg ctttctttct
    22981 tcacgcttaa ttgccgtttg ctttgcttta tgcttcccac aatacttctg cctataatca
    23041 atcaatgttt tacatccagc atgattacat tgttttttaa ctgccataga ttaccaacca
    23101 aagcgatctc gttcgtcaga atcatcttgt tcccattttg atttgtattc aatataaggc
    23161 gaaactatct tcaagcacgt tccgtcttta aaattaactt tagtgatttt atcttcctca
    23221 aaactaataa agtctacatc ttcttgagtg tttgaatact caatgtgttt ctcaccatcg
    23281 aagtatctaa tactttccat atgcataatt attctacctc caataaatta gttatcaatt
    23341 catcatctgt tagtagcaaa gccaagaact tatcatggat gtttaaaaac aaattatcct
    23401 cattcgatgt aagctctcgt tgtctcgctc gatcatatag gttaagcaga caattaaatt
    23461 catccattag ctcacctgta tcatcatagt tgccactatc taccaatgat cccactacta
    23521 gcgattgaca ccacgctact gactcctgca gccaatcagg ttcttcttcc attatcttta
    23581 atacttctgg atcaaatgtg gaataatctt ctgttaccaa tcccacacta aatgccagcg
    23641 tgttcattag aaaccgatcc atttcatctg tatattgaaa gttcggtgct ttgtttgttg
    23701 aaataatatt gagcatttac tttcctcctt gattcgtaac acgtggtaac acctacgtaa
    23761 caggtacccg ttaccactca atcccaacgg ggacaagggt tctagcgttt tggtaacagg
    23821 taacgggtaa aatccagtaa cattattccc cagcatatta gctaactatt tcaaaagttt
    23881 taaggaaata aggtgttacc ccgttaccaa attgctgtat cccttggggc agtaagggta
    23941 aactagtaac gggctaggtg ttactgaccc gttactaccc gttacttttt gtactttcca
    24001 gctcgatttt tattttcata ggcatttgac ttattgtaaa atcctcgtcg tttatttctt
    24061 tttggcagtt gcttccgttc ccaaccagga atactgctaa taatcaaggc aatcttttta
    24121 gtttctgcat ctgatcgacc attcaataaa tcctttgctt gtttatcaaa aacagcttcc
    24181 aaaatatcag cggtgagtac gctatcaact aaataaaact cttgttgcat agtagctcca
    24241 ttatagaaat gctgtaaatt tttctcatta catttactgt caggatagtt ctgataataa
    24301 gaacgtctcg cccacgctgg agcttcttcc catttatacg ggatttccat ttccaaatat
    24361 ttagtgattg cttctttaat tggattctcc atcttggcat cctcttgata gtcttttgca
    24421 atttctaaga cttctttatc ttcatcggga ctaaaataga ttcgttgtcc tttatcaaaa
    24481 agcacctttg cttcagctaa cacttgaaga aaatattcat cctttgtttt aaacggattc
    24541 atctttggct tattcttaca aggcaaagga taaaaacggc ggtttcctgt atcatctttc
    24601 aaatactgcc catcattcga cgttccaata aatacacatt gccttgccca agctatgaca
    24661 ttccgaccat aaggaggtct aattttatcg atcttggcac tgatgaagtt ttttatctgt
    24721 tcaattttag ttttgttcat tgaagataat tctccaagtt caataattac attgccttga
    24781 agctgcatat aatcatcttt cttttcacct agcgaatcta atgaatcggt gaaaaaatca
    24841 tccgtgtaaa gtaatgctgg aacggtactc tcaccaatcc cttgcggtcc atccaaaact
    24901 ggaacgatct caaactttat tcctggttta tagatacgtg cgactgatcc gactatccaa
    24961 cgttttgtta cttctcgtgt gtaaaggttg tcgtctaccc ctaaaaggtc aataaacagt
    25021 gtttccagtc taggctgttg atcccattta actttttcga tacgttcttt tactgggttg
    25081 aaaacattct ttctagccac atttactact gctttgtgta ttttgttgtc atcaaaaact
    25141 acattgtagt tttcttcaat atatgccgtt aaggcatcct caaaactgtc ttcccattct
    25201 ccagcggtac gattgatcca aggagaatct tttaataggt gaatgtatcc gctaaattcg
    25261 ttataagcaa ttgttccagc taaattgcta tcattggtta aaatcaattc tgcattcaca
    25321 acactgttac ttttaatctg atcgtcttta ttgcatctta atcgtgacat ccaggttttc
    25381 aattcaatga tattgtcttc acgctcgctg gcttcgggaa agtctaaaat actatctaat
    25441 ttatgcttat gagccatgag ctttccccct caaatctttt ttaatcatcg atcgaataat
    25501 ttgattcact tctttatctt ttagcggtgg ttcattgtac gcatttgctg tataaatcaa
    25561 ttctgctaaa gtttgattat ctacaccgtg ccatagtaac caaccgcata acttagttaa
    25621 atgatcgttt ctactacctt cgccagcgcc ttgaacaatc atatctaatt tttttgctgt
    25681 ccctgttttt gctacgggtc ggtattgagt ctttatttct tgcggttttt ttagcagtag
    25741 gttcactagc caatcgggag ctggtgcaat attatttaga gatacgtttt caataggttc
    25801 atatgctctc ccaacacttc ttgaaataat catagttggc gctattggaa ctcctttagt
    25861 gataatatca atcccgctta aacttttacc tttatcatca cgcccgaaga tatcagcaaa
    25921 tgcacctgtt tcttgttgga taattacacc atcaggaatt ttaaagaaga aatggattcc
    25981 tttacctggt gtgatttccg tataagtaga aggaaatgga tcatacattg aagcaagttt
    26041 tttataattc tcaacgccgt ttcgtgtttc agaatgttga tctaggtcaa ctaccaccat
    26101 ttgatgatct tcaagcatta acccaatatt tctagttgca tcttccgtcc accattcatt
    26161 aatttgatta gaatcgttag ttgcatcatt ccaaccacta gagcctttta ttggaatttt
    26221 agttccagga gataagggat aaattgacca tcccgacttt tgtaattccg ttgctgcttg
    26281 ataattattt gccaataaac ctctcctcct ttttcaaaat actggtataa atcattcaga
    26341 cacctccatg agcttcaact gctcaagttc gtatttcaac ccatcccgaa caaattcagc
    26401 accttttaat tgattctgat actttaccca tgtattaaat gttttcaccc ttgcttcttg
    26461 catatcagta atgtttaacc atgcggttaa aatctgattt tttagctcgt tgatttctcg
    26521 acaaattatt ttgtgttctg catgtttttt gagtacatac aaaactgtta gtttttcatt
    26581 gaatgaattt ccaaagtagg ttttcccgat attattttct agttcagcga tcccttgatt
    26641 aatttcctct aattcaaggc tatcgagttg gtttaatagt tttgttttgt acatctactt
    26701 atgccctcca gttcttaatt gatagtcgta ctcgtcataa gcagtgaaga gatataacgc
    26761 taatcctgca aagatagcta tttttgcaat taatggtaat aagccgaaaa ttccgccgat
    26821 taaaatgctt cctataatta agtttcgttt cataatggtt ccctccgatt ttttaatatg
    26881 gtataatcgg aacaaagaat atcgggtttt atcccaatta agttgccttt atcctgtccg
    26941 ccaagactgt tatggataag gctttttctt tgttctcgat catgttaatg ctcctaagtt
    27001 gtctgcaatt cagcagaacg attataaatt ttctcactca ctgaaaactg caacccatat
    27061 ttttctttaa cagtcattaa ttctactgtc tcatctaaaa taggttcacg atcaaccaac
    27121 atttcaggag tcatatcttt cttttttacc attttggaat gaccataaat agttgataca
    27181 gctttattgg ctatcacatt cgctttaatg tagtctactg gttttggttc agtaatagcg
    27241 tggcttaatt tagtcattgc ttctttttgg tgttctttgt ctagcatgcg gaatacttga
    27301 aagccttcaa gtcctgtgga ctggcgtagt tctttgatga tgtcgaaaat ccagtcttga
    27361 aatgcctcgg cttctttttt acggcttttg aatgccatgc ggtaaatatt ttttttcgtt
    27421 gatgatgttt acatcttgtg ttctgcccaa tgaatcgatg accttactag tagtaacccc
    27481 atctttaggc aaagagtgaa tggctctagt aacctgttta agacctaacg cttccgcaac
    27541 atctttcgcc actgcccacc attcatcatt aatatcgaca aatcttataa tgtgtccgtt
    27601 ccaaatttca gttttcattc aacgtcctcc agttcaagcc ctagcaagct cgctaacaac
    27661 tcaacttgct gatacaaaat ttcttgatac tcttcaatag aaagtcgttc tttaaactct
    27721 gcttgtacta aatcgttcaa gccacgcaat actccgctta ccgttggttc ttgtggtagc
    27781 aatttttgat agtccatttg ttcgtacaaa cattcaaacg tttcttctag cgctttgcct
    27841 tccaagtttg ctaattctgc cgctgtgtct tctgtaagaa ttgttgataa tactttttcg
    27901 attgctttat tttgtgtcga ttccattatt atttcctcct attaatccct ttgcccccac
    27961 ggcttgcgtt tatttttggt attcttccat aaagcgatca cattcatctt gtgaaattct
    28021 tacgactcca tcaactataa tcaactttaa accgttagga acatatttct gcatcatagt
    28081 attgtatgaa acgttcatat acttagcagc ttgtgccttg ttcatatatt ttggaaactg
    28141 gtactttttt tctaataatg aaagtaacgt tccttgagtt tgctctactt gttgaacaac
    28201 tcgatcgtca ataattttaa aagctggatc atcaagcgtg agtttgtcaa tttttaattc
    28261 catatttatc ccccctcgtt agcatacatt tctatacaaa gattttaaaa aaatgcaaga
    28321 ggctcttgaa tacatttcta tacctcttat aaactcaata taccatcata ccttttcatt
    28381 tgcaagaatt ttaacatact tttttgaact tttatgtttc acacatattt aaatcgtgat
    28441 atagtagaga aaatatatga atggtggtga aaatgtggct agaaaaaaat accccgaagc
    28501 agtggagaca ggacaaagaa ttagactggc tagaattgaa aagggactct caatggaaca
    28561 acttggaggt aaattatccc caccggccag taaaggtgcc gttagtaatt gggaaaatgg
    28621 ctataatctt cccaataaca ccaggcttaa acaactttca aaggtattag atgtatccac
    28681 tacctatcta ttaaacggat cttatacatt aagagatatg catatgatgc ccgaaattaa
    28741 gcgaaatgtg cttgaaaagc aattgaaaaa aactattgaa aaaaataact ctataattga
    28801 ttctaatctt tctaatgcta tcaataacat cgaagaagat aacctgactg tctcacagaa
    28861 gatttattta acaaacgcaa taaaatatct taaaacagga tctcgaattg aatttcctga
    28921 cgggagtacc ccaattgctt atctcgcagc tcttctaaat acgcttttac gatatgactc
    28981 attaattaat gattcagaaa aaaatacaga tgagaaaaaa gagtatcaag aattttatga
    29041 aaatgatctt ctaaaagagt tcacagctct tgttactgag ctaaataaac actattcaaa
    29101 ataataataa actgcctcct ccaaccgccc ccacggcttg ctacactatg gagggaaaac
    29161 tattatggca acattcaagc aatatgaaac taaaaaaggt aaattttggc tctatgaagc
    29221 ctatcttgga atgaacaaaa tgactggcaa acctgataag gttcgtagac gaggttacaa
    29281 aacaaaaaaa gaagcccagc ttgccttgtc acgcttgcaa gtggactacg ataaaaacgg
    29341 tattaaaaaa gcgaacaacg aaacgtttaa agaagtttat gacttgtggc tagaaacata
    29401 taaaaccact gtccgagaag ccaccttcat gaaaactgaa atcaagttta gaaagtggat
    29461 tcttccaaaa tatggaaatc ttcgtgtgaa tgaagtcacg gtaaaaaaag cccaacaaat
    29521 tgtaaatagc tgggctgaaa ctactgacca atacaaggta cttcattcaa cttctaagcg
    29581 tatttttagc tacgctatca atcttgggat tattgagact aatccactag acaacgtatt
    29641 aatgcctaaa agaaccacca ctaagcataa agaaaaggtc aaagtttaca gtaaagaaca
    29701 actgatcacc ttgtttgatt acctaaacag caaacctgaa acttatcgaa acgactttga
    29761 taaaacgtta ctacgctttc tcttttatag cggggttagg atcagtgaag cccttgcatt
    29821 gaattggtca gacattgatt ttcaagaaaa tacggtcacc atcaataaaa cactaagcca
    29881 atccaaaaat ggctataaaa tcagtgatcc taaaactgat tgtagtgctg gggttcttcc
    29941 actagatgaa agaacaattt ctatattaaa aaaatggcag attaatcagc ggaaatatat
    30001 gctgacactt ggtgtaactg atccgactat gattttttgt ggtatctata aagaactaat
    30061 cacacaccac gctatttatg caagattgat tactatttgt gaaaaagcta tggttccatt
    30121 tctcggtgtg catgtagcaa gacacactca tgcttcaatg ctcttggacg ctggagctac
    30181 tatggtagaa gttcaaacta gattacggca tacaaaaatt agtcagacga tcgattgtta
    30241 cggtcacttg gcaaaagaaa cgaaagaaaa gactgttgaa aaattagtac aacacttaaa
    30301 tattgactag atggcataca atatggcata cagtaaaaaa taaaaaaagg agaaacccta
    30361 tcatatcaag gtttctccta gctttatagt ctattcttct tcttccgcca tgaatgtatt
    30421 gaatacttct tcaatcatgt cccattctgc gtctgtttcg atttgttcta actcgccttc
    30481 tgttccatct tcgttttcga tgtatgcata agcttgtaat tctacatctt catcttctga
    30541 aacaccagca ggatataaaa gaacatagtt acgtccaaat tcttcttgtc cgtcaactgt
    30601 tagtaggatt tcatataatg tttcatttcc ttggtcatcc accaatgtga tgtgttcgtg
    30661 tccttcatgg tcgtgggaat ggttatgttc tgtcataaga ttacctcttt tcttttatta
    30721 ttaaaataat tgcttacctg agccgtctaa ataattttgt aagatcatca cagctgctaa
    30781 tttatcgatg actttttttc gtttggctct tgaagtattt gcctgctcta ctagcatacg
    30841 ttctgcctga acagtggtta atcgttcatc ttgataatca actggcaagg agaatagttc
    30901 tttgatcttt tctccgtatg acatggaagc ttctgccctt ggtccgatgg aattgttcat
    30961 atttttaggt aatccaacta caaaccggtc tacttcatat tgtgctacta attccttcaa
    31021 ccgatcaaag ccaaactctt cttctgcttc attgatccga atgatctcta ctccttgagc
    31081 tgtccagcca aaggggtcac tgatagcaac acctactgtc ttagaaccga catccaagcc
    31141 cattactctc ataaattcac tccatgattt gagaggtagt ccttgacaat tacttccatg
    31201 atctcatcgc gttcgtgacg gcgaatcaaa tttcgagcat cacgataacg aggaatgtag
    31261 gccgggtctc cagaaagcaa gtagccgaca atctgattga ttggattgta gcctttttct
    31321 tccaaagccc gataaacaat cgctaatgtt tcgctcactt cttttttgcg actatcgtca
    31381 aaaccaaaac gtacggtttc atctgtaaaa cccatgattc tacacctctt tcccgcaagg
    31441 taattccagc ttaccttcta caaccttatt ttactcgaag aaagggaaaa ctacaaacaa
    31501 atattccaaa cttccgaata aattaagaaa cactatttgt atgttttttt ccttttttct
    31561 gtagctttat caaaagtact cttttcataa agtatacgct atatttcttt gaatgtatgt
    31621 ttttacacaa ttttttcata atattcataa aaaattcgaa tagctgctac taattttttg
    31681 tttttgttgc ttgtcctgtc tcataattga tcgtgtagcc atcttgaaca ttgtagataa
    31741 agacattgaa ttcaattttt ttgtcttcaa tgctttctgc ttccaattgc actcccctgg
    31801 ctaccagttc attcccctca aaatgaggag tgacacggta gcgtacatga tgatttgttt
    31861 ctttgatata tgccgcaata tcattttcgt gagcaagcat gtatggagca ttcaacgaac
    31921 gtgtccctgt catcaagttt ttaatgttgt tattttgacc agtcagctgg aaaccaatta
    31981 aatggctgcg attatataac cattgcccgt cacttaattt cttttgtttc caacctgtcg
    32041 ggtcaatata caaaggctct ctcttttcag tagggaacaa atcttttcca agcatagcat
    32101 tggcttgtcc tactctgttc aatttatcaa tatttgaata gctttcccat gttcctttgg
    32161 aaaggctaag atcctctttt gtaaattccg gctgattccc attgatttct atttcttgat
    32221 gttttccatc atattctggc acatccgcta cttgagaagc ttgtggtgtg gaatcaaaaa
    32281 tagttgtaat ctgctgaaag atatctgaat ctttttgtgt ggtggaaaca ccgaatgccc
    32341 ctgcaatcac agcagcgacg atccccacga tcgccttggt tgtttttttg ctcatttctt
    32401 tactccttta ctaacagctc cctaaatctt aacatatgtt cccctataaa taaatactag
    32461 aaagaaatag aaacagcaaa aaataaaacc tgttttaaga tggcttaaaa atacaaaaaa
    32521 gagacggatt cctcgtctct aatcagtcac cactctatct gtaaggtatt tattattatt
    32581 caaaaatttt cttcatactc aagacaatac actgatttca caaccgcttg gtaaacggtg
    32641 cgcaaaagct tgcatctgcg gtaataagtc catccaagtt aaaaacataa gaagttgttt
    32701 ttgcttggct gttcttatta cttgttgcag agcagctttt tcacaaccta ttttaagcgg
    32761 gtgacgggaa tcgaacccgc gacatcagct tgggaagctg ggattttacc actaaactac
    32821 acccgcaata caaaaacagt atacgcctct tttattcctt cctcaagttt ttttattctt
    32881 tacgcttctg gtatgttttg tagtaaaata aaactaatca atcaattatt ggagaagatg
    32941 ctatgacggg aaaagaatta gatctgttgc tggacacttg tttactcgct gggaaaatca
    33001 tgatggaaag caatgcggag atgtatcggg tagaagatac gatgagtcga attgctttag
    33061 cttctggaaa tttccgtttg gtcagctatg tcactcaaac cggtctcttt attggtcttg
    33121 atcgtacctc gaccatccga atggaacaaa ttaccaaccg ttcgattaat ttagaaaaag
    33181 tagtaaaagt aaatgatctt tcacgtaaat atgttgcttc agagctaaca ttagaagaac
    33241 tttactgcga actgcaagca atcgaccgag accgttcttt ctttcctgtc tggttgcaaa
    33301 tcgttagcgc ggcagtgatc agtggaacaa ttatggtgtt atttggcgga aagatgaccg
    33361 atttacctgt gactttagtc attggaggat tgggatacgc agcttatcta tatagtttga
    33421 aattttttag aatcaaattt ttatcggaat ttttatcttc tttattgatt ggcatagccg
    33481 ctatctacag cgtacgtttt ggcttaggaa gcaatcagga tctaatcatt atcggatgtg
    33541 tgatgccttt agttcctgga gttcaaatta cgaacgctat ccgtgatttg ctagctggac
    33601 attatgtctc aggagtttct cgcggaacag aagcgatgat gactgctacg atgatcggtt
    33661 ttgcgattgc ttttattttc cagctttttt attaggaaag gaacacaagt atggggacga
    33721 tatttattca gttttctttt agttttttag cgactgcggc atttgccgtt attacaaatg
    33781 tacctaggcg ttcattgatt tgttgtggtc tttctggtgc ttttggctgg atgatctact
    33841 ggatctgtgt tcaaataggc ggatctgctg cttttggttc attgcttggt tctttaggag
    33901 tagcattcat cagcgatctt ttctctaaac gtttgaaaat gcccgtaacg attttcaata
    33961 ttcccgggat ggttcctcta gtccctggtg gtttagctta tcaagcggtt cgaaatctcg
    34021 ttactgaatc ctatcgagaa gcggctcatt atgcggtcca agcaattatg attgctggag
    34081 caatcgcttt aggtcttgta ttatcagaag tctttaacca taacatccgt aatttcaaag
    34141 taaagcgtga cgcaatcatg atgaaacgaa aaaaacggct ttttaaataa acgaagtgta
    34201 acaaagaaaa ggttgtgaaa gagacgttca gccttgagcg atgacaaaat atctcacaaa
    34261 cattgcgaca agtaaccgca ggagcaataa gaagtaatta tgagtattaa aaaaagtttt
    34321 gtaaaaatag cttctcacat ttttacataa aattgactta atactgagaa actgactttt
    34381 gaacaccctc tatctgtaaa aaaggggctg cgacatgatt tttgtcacgg cccctcttct
    34441 ttattcaaat tttttccaaa acgttcgttg ataataaacg atccatagag cggaaagtat
    34501 tagaactaaa ccgatcacag gaatattcac taaccaaaat ggataaaaca aagccaacat
    34561 tccgtataag acgataagaa taatactgat gatcaatgaa cccatcatag ttgcgactaa
    34621 acccaaactt cctgaacctc tgttaaataa ttggttaata ttggtccagt tcagcagcag
    34681 caaccgataa tctctagcga aataacgtag tgacaataag tagcagccga ataaagaacc
    34741 aaatagcaag ctagggataa aaaacaaagg cagctgaaaa gacagtccgc ttaataatgc
    34801 gatcccacca gttaaaaaac tctgcaatat ccatccgaat ctgaactttt cttttaaata
    34861 ttggttcata gagattggca aagaacggat aaacagaaaa ttttcttgat ccaaagaaat
    34921 aagattgctg ataaaagaag tctgattaac tgttaagaaa gccaatgcga tccctaccag
    34981 gaaaacaaca ccgattaact tattatctaa atcagttaat tttatttcgc ctcctagggc
    35041 aaaagcaaga ataaaaatga ttggagttaa caaggaagaa gaaagaactt gcataatcaa
    35101 attaggttct tttactaatt gagcattgta actaaataaa agctgatgca cattttggtt
    35161 ctttttatgg gttcttttta ttttgaactc actgtttgaa tcagtcagtt gctctgtaag
    35221 ctttggtact atcagctgtt ttacagcaag gaaaaatccc agagccgtga tagctagtat
    35281 tccccaaccc aataaacctt gttgagaaag tggctgactg gcagcatcat agaatggcaa
    35341 taatatcgaa atcggatgcc gatctgttaa ctcattttca atagtccccg tttgatgatt
    35401 catccacatg attccagcaa atgcgatcag catcgagctg ccaagcaaga tactagaaac
    35461 tgtttttcta tgttttttaa acactgctgt tcgagccaaa ccaaaaacaa tcaaactagc
    35521 cagacaaaaa agaataatca aaaatagaga aaacaaaagg attgagataa tgatagtaag
    35581 caaaatccct agatcagctc gccatccggt taaaataaac atgactagaa gcggaaaaac
    35641 aaaaggaatg atggtcaaag caactaccaa aatctttgct gaaaaaacct gttcttgtcc
    35701 aaaaggcaga ggcaaaaata cttgcaggtc atttccttca aaaaagatat tataaatgac
    35761 tgatatactt tgagttaaac ctaacatacc aaaaagtgcc acgtaataag taaagaaacc
    35821 tggcaattta acaaagttca tagcaaacat agcaaatccg taaatcagta aaaaaatcag
    35881 ccctgaaaaa agattttgag acaaaagata gatagttaat cgctttccct tttttccttt
    35941 ctctcttgct ttattcgtga gctgtggatt aacatataaa agattaactc gaattaattc
    36001 taataattgt cgcttataca tacggtccat ctcctgtcgc ttgccttcca gccattttca
    36061 aataaatctt ttctagcgtt tcatcaggtg agccattcaa taattcacta actgccccct
    36121 gataaatcaa ttcacctttc ttcaaaatag ccagtttatc gcataactgt tgtgcggtat
    36181 ccaatacatg tgtagaaaaa atgactgttt ttccttttgc ggcatgagcc ttcatcattt
    36241 ctttcaaatc aaatgctgct tgaggatcaa gtccttgcaa aggttcatcc aatacccaaa
    36301 tatctggatc tggcagcaac gtgccgatca aaatcgtttt ttgacgcatt ccatgtgaaa
    36361 aacttgcgat tgtttcattt tgatgactat acatatcaaa aagcgctgtc aattctgcta
    36421 aacgcttttc ttttttttgt gtgtctaatt cataggcagc tgaaatcaag tcccaatatt
    36481 ctccagccgt caattgtaaa aaaatatctg gggaatcagg cacataaccg attttttgtt
    36541 tgatactcaa tcgattttcc gtcaatttca ttccatctac taaaatcgtt cccgatgatg
    36601 gttcaataat actaactaaa cttttaatcg tagttgattt tccggcacca ttatgtccta
    36661 aaaatccaaa aatttcccct tgtcttatcg agagagacaa gtcttttaat gcttgttttt
    36721 tcccgtaact tttacttaca ttgatgaatt caatcatttc tgtactcctt ttgtttgtta
    36781 ttacttttta ttgtaccctt cgttattggt ggagtaaatg tcaaaaagag tgcataacaa
    36841 aaaaggatag aaacgactag gctgctactt gggttgttct cttctacagg aaaacactct
    36901 tctaacaggt aagatatgag tctttgtttt ctttaaaatg attcattttc tatcttttct
    36961 tgcataaaat tctttagttt atcaatactt ctctttccca tctctagttg ttctccattt
    37021 gcaaatgtta ctatttcgtt tactctgcta atagatgtaa tgttttcagt attaatgata
    37081 taagctttta aatctttaca taaatactca tattcgagtg attttttata ttttttaatt
    37141 gtgccgttta cgagaatctc ttcatctttc aatactaggc ctattttatt tttataacct
    37201 ggcatgctca ttagataaag gatttcatcg taatggatat agtgatcgct attaccataa
    37261 cggacagttg tccacctttt tttcgtagca tgttgaacag gatagttttc tttttctttc
    37321 aataagtaac tttggatgct ttttttattt ttttcataag tgaattcttt tatcaaataa
    37381 ccactaggcg taatctttcg attaataaca gttattgctt tattcggatg agacgttaga
    37441 taaacaattt tacacttttc attcttttct ctaagcattt cgcctagttc gataccgcta
    37501 taataggcgt tcaaatcgat gtctaggaaa aaaatatctg aggaatagat ttcattattt
    37561 ttaatgaatt caacaaattg tataacgttc aatccctgaa cgatctcaaa tttccaagga
    37621 cttaataaaa ttctggattc ttccaaaatt tctactagca ttttttcaaa aaccaaacta
    37681 tcatccaaaa taaatatacg cgttaccata aaaagtacct ccactcgata ttttactatc
    37741 aaaatcattt catacaactt ttcaacatac atgttaggaa taaattgtaa ttctcttcac
    37801 aatcttttcc aacacctttg aaaccgatat caaatacttt caggatgctg cttctaaaat
    37861 aaatatcaaa caaatgaata catcatgcaa aaaaagccag acaaccgacg tccttgcgtg
    37921 tcagctgtct gagctatgtt tatttatttt gttcttttaa aaagaacgat acacatactg
    37981 atttttcgga gcagagactt tctcagcgtt tgtaacaact acagatggga tttcacgctt
    38041 gaatggttga taatagtctg attggatggt tccttctatt ttataccatt catcattttt
    38101 aaccgttact ccttccggca tatgcgtcaa caaaccaaag actcctgaat ctgccacaca
    38161 atggataatg ccgaatcgga acaagaagat atctgtattg ctatcgttcg gtgtttggta
    38221 aacaaatcct tcatagctga tttttttgcc aatgaattcg ctagggtaat tataaattag
    38281 ctccataact tctaaatagt tttcatctgt gatcttgata gtttgcttat ccccatactt
    38341 ttctttcaac tcattcattt ggtctaagta gtctgatttg ttgaaataaa tactggtatc
    38401 cggctttaag tattgtgtct gcatctccgg atcccctact gattctttac ttactgggaa
    38461 attaaatcct ttggcttcta cgatcgtagt gtcgagacta actgttggaa acaacgtccc
    38521 cacgattact ggcaaagcta gcagtacata agcgattcca cgttgataag gttttgacaa
    38581 cccatggtca tgatcgtgct cgtgtgcctt ttcttctgct tttccgtttt tgacccacaa
    38641 atacagctga actaaagcca aaagagcaga taaaatcatt gaaagaatag ctaagtagcg
    38701 ataatggaca ttgatatatt gatcgagttt gccagaaact tgcaagtaca tcatcaattc
    38761 gaaataccca gataaaatca aaaaccgcag catattttcc ctcctaaata aataatgtga
    38821 atccaataac gctaagggca atcagtccga tcagcgataa aataaagcgt cctgtaaaat
    38881 atcgtttcat catcagtaga tttttgatat cgaccattgg gccaaagacc aaaaaggcag
    38941 ctacaggtgc tgtgccgaac agacttaaca aagaagatcc tataaacgca tctgcttctg
    39001 agcaaagaga catcgtaaag gccaataaca gcatgatgat aatggctaaa atctttgttg
    39061 agccgagcgt tagcataatt ctagttggta aataagtctg catcgcagct gcgattaatc
    39121 cgccaatgat caaataacga cctgtatcaa aaaattcgtc gattgaatgt gtcaacacat
    39181 gcccagtttg atggatcagc tgattttttg gttcattctt ctcactttgt tcttcttcat
    39241 ggacatgatg atggtgattt tctgctactt tagacgtgat tttttctttc aatacagcgt
    39301 ctttttgaaa ataggcaagc cagacaccaa tcacaagggc aacaaaaaag ctgcctatca
    39361 cccgccagct tgccatctgc caagaattgc caaatgcgat aaaagtggaa aaaatcacga
    39421 ttggattgat gatcggagca gtcagcataa aagcaaacgc tgtgtgaact ggtacgcctt
    39481 ttttaacaaa ttgatgaacg atcggtacga tgccgcattc acatgaagga aagaaaaatc
    39541 ctaagatcga accagtaaaa atagagaaga tacgattttc aggaagccat ctcttcacac
    39601 gttctggggt caaaaatact tgtaaggcgc ctgagataag acagcctaat aatacgaaag
    39661 gtaatgcttc aataacgatc gataagaaaa tcgtactcat ctgcaaaacc gaatttggaa
    39721 taaaagaaaa catgagtttc ctccttttta tttctagttc attcgctttg caaatatacg
    39781 cttttattca aaaaaaacaa gttgctctgt aaatttttac acaactctta ttttttacca
    39841 aagcacttca attgactttt tctgttattc ccattaaatt aaggtctcat acatatcact
    39901 tctatcataa aagggagaat aaaaatgaaa caaaccacta acttgactga agttcaagac
    39961 atactgcaac agtcatccgt tgccgtcatt tatctttcga tgcctaattg cagtgtttgt
    40021 cacgctgttc gtccacgact agaggaactt ctcacacatt atgatattcc ggcacttcat
    40081 ttggatgcat ttgaaacacc agaagtagct agtaaatttg aagtgctgac tgcgccagtt
    40141 gtcctgattt ttcatcaagg aaaagaagtc tttcggcaag cacggttcat cgactttaaa
    40201 aaaatccgtt atctactaga tgaactgaca gcagaagatg attccctttc ttatgaagaa
    40261 atcttccgaa caaaataatc gttgtccaaa cagaaaaaag cgattgaaca agtgaaagtt
    40321 ttgccttcat ttgttcaatc gctttttatt tttattctgc tgtattttcg gaatgcgcat
    40381 ttgcgtcaga atgcaagaaa tagatcaagg tttgcaattc attcgtcaaa tcaacatttt
    40441 ggatcaatac atctgacgga gcattcaatc gagcagcggt aaagttcaag attcctttga
    40501 ttcctgcttc cgttaactga ttgactactt cttgtgctct ttcagcgggt atcgttaaaa
    40561 ttgctacttc gatttgttgg atcttgattt gttctgacat atcttccatt ggataaacgg
    40621 ggataccgtc tacgatgcgc ccaacgattt cctcattcac atcaaaagca caactgatac
    40681 gtatgctatt actttgatgg aatttgtatt ttagtaaagc acttcccaag ttccctacac
    40741 cgatcagcgc aacatttgtc aattgatcgt cgtttaatgt ctttgcaaaa aagtgcataa
    40801 gatcttctac atcataacca tacccacgtt ttcctaattc gccaaaataa gaaaaatctc
    40861 gacggatcgt tgcactatct acttgaacgg cttcacttaa ttcagttgaa gacacttttt
    40921 tctttccagc attatttaaa atccttaaat agcgataata caaaggcagt cttttagctg
    40981 tcgcctttgg aataatatga tctttcacga aatgacctcc acgtattgtc taattacatc
    41041 tttgatttct ccatcatagc agtatttata ggaaaaaatc aattccattg ctcagatatt
    41101 gtgaaatttt attctttatt catttattta tcatttctgc aaatctcgca gtataatgga
    41161 atttatggta gactactaat tgaaaaatag ttttttattt gaggagaatt cacaaagtag
    41221 aaaagaggat tccatgatat tattacaagc caatcagatt gctcgccatt ttggtgcaga
    41281 agtcttattt gaaaacatac atttagaaat cagtacaggc gctcgtgtcg ctttagttgg
    41341 cagaaatggc gctggaaaat cgactttatt aaagatcatt gctggaattg aagcacctga
    41401 tgaaggaaag atcgctaaaa ataaaacagc gactctcgga tacctcgctc aggacacagg
    41461 acttgattcc aatgaaacag tttggaacga gatgctgaaa gcttttgaac aagttagaca
    41521 aatggaagag cggatgcgcg agctagagat tttcattagc gaaggtacgc cagacacacc
    41581 tgcttatagt tcttatttaa aagaatacga ccggctgcaa cacgattttt cagatcgaaa
    41641 cggctatggt tatgaaaatg aaattcgttc cgtattgcat gggtttaaat tcgattcttc
    41701 attttatgat cagaaaatcg atactttatc aggcggacag cgcacacgtt tagctttagc
    41761 tcgcatgctt ttgcaacacc ctgacatttt gattctagat gaaccaacga accatttaga
    41821 tatcgagaca ctttcctggc ttgagaatta tctgcaaagc tatactggtg cattattgat
    41881 tgtttcccat gaccgttatt ttttagataa agtagtcaat gaagtctatg aattaagcag
    41941 aagaaaaatg cgccattaca aaggtaacta ttcgaaatat ctagatttga aagcagaaca
    42001 gttagcaagt gaatggaagg cttatgaaaa gcagcaaaca gaaatccata aattagaaga
    42061 cttcgttgct cggaaccttg ttcgcgcctc taccacaaaa cgagcgcaaa gccgcagaaa
    42121 gacattagaa aaaatggacc ggctggatcg tcctcaaggg gatgaaaaac aagcaaactt
    42181 tctattcagt atagaaaagc cttctggaaa tgtagtcttg caaatcgaag atgccgctat
    42241 cggatatgat aatgtcacct tatctgaacc aatcaatcta gatatccgtc gtcaagaagc
    42301 gattgctcta gtaggtccga atggtatcgg gaaatcgacg ttgctgaaat caatcattca
    42361 ccagatccct tttatccgtg gaaaagaaac gctagggaca aatgtcacca tcggctatta
    42421 cgatcaaggt caagctgatc tgcatacaaa caagacgatc ctagcagaat tgtgggatga
    42481 gcatcctacc actccagaaa aagagatccg aagtgcttta ggtgctttct tattttctgg
    42541 cgaagatgta gaaaaaccaa tccctctgct aagtggcggt gaaaaagcac gggttgcttt
    42601 agccaagtta gcgatgaaca aagaaaattt tttgattttg gatgaaccaa caaaccattt
    42661 agatattgac aacaaagaag tattagaaaa tgcattgatc gattatgacg gcacactgct
    42721 ttttgtttcc catgaccgat atttcatcaa tcggatcgcg acaaaagtcg tcgaactctc
    42781 tgaaaatggc agcaaagtct atctaggaga ctatgattat tatttagaga aaaagcagga
    42841 agaagaagaa atcgccgaat tgttggcaca agatgagcca gaatctgctc ctgctcccac
    42901 gaacaaatct gattattacc aaagcaaaga acagcaaaag cttatccgta gccttcaacg
    42961 aaaaattact caggttgaag aagaaatggc gcggatcgat gaatggatcg atcagttgga
    43021 acaagaaatg gtcgcgccag aaaacctgga agatcacgta aaactcaatg agctgaacca
    43081 agatttagaa gctgctagac aagatcaaga aacaaaattg acggaatggg aagaactaag
    43141 cttggaatta gaagaatatg aaaattaacg aaaaaaccaa aacagaaacc attcatgctg
    43201 ttttggtttt tcttttttta ttttatcaaa caaaaattcc atatttacct ttttcaccaa
    43261 taagaagacc aattactagt cattctcact aaagcttcta aataatagta atcaccccaa
    43321 atacaaggtg tatttacccc attatttttg ggcttattat aaacagactg tgttaaaaag
    43381 ccatcttctt cttccgttgt agcatatctt tctttcaatt gtcgcataat tttccaaaca
    43441 gcagactggt ataagttctt ttctgttttg tctaatagac ctgattctat taattctaat
    43501 aatccacaaa ccatgatcac catactagat gtatcttttt cttctctgct agaatcatca
    43561 aagatcaaat cccaataaca aatggtatct tctggcaacc gattcataaa ataattggct
    43621 aatattttgc ttaaatcaaa atatttttgt tctttcaaat aacgataact taatgcaaaa
    43681 ccgtaaatcc cccatgcttg gcctctagcc caacaggaat tatcactata accttgatgc
    43741 gtacttccat atcttggttt tccatctttg acatccatat aaaatgtatg ataagttgaa
    43801 ccatcttctc ttactaaata atctgctgca ttttgaatat gagcctgagc agctttttta
    43861 tatttttgat ttccagtttc ttcactcgcc caatacaata atggaagatt catgcaacaa
    43921 tccatgatca ttctaccttg ttgtgaagga tcactaggat ctccccaagc ttgtataact
    43981 cctgccttct cccaaaaacg ctgaagtaat gtttctgcag catcaattga agcttccttc
    44041 atttttcgat ttcccgttat tttataatct gctacggttg ataaagaata caaaaaacca
    44101 agatcgtgat gattagtgat aatttgacag tcaatcctgt ggataaaatc tatatctgct
    44161 ttttcagctg tttgttgaaa taaaggatca cccgtcttct caaataacaa ccaattaatt
    44221 cctgtccaaa atccttctgt ccaaccaata ttgtctctta atggataacg tccttttata
    44281 ggacactcgc ttgggaattg atactgactg ttactgatac gtttgattaa cctctccgtg
    44341 tgtctgcgac aacgctcgat ctcattattc cattcttttt ccattaattc tttataacat
    44401 tcatttatcc atttttctgg atgtattatc tcttcaaaca tcactttatc aagcatttcc
    44461 ccaccagcct ttagcataat tttagtcttt ttttattgtt tttattttgg atagttagcc
    44521 cggttgtccc atcaaaatca attttgggac gattgttcca tacttctaaa ctataatccc
    44581 gatcttgatg agcaaccact cctgttccga gccaatgggt tccttttcca aatgtaccct
    44641 ctaatccagg aataaaagtg ttttcccatg tcatcaagtt tgtattgggc aaacccataa
    44701 tagctgtgga ttttcgttta atagtatcgt cacttaaaca gataatacct gaaaagccag
    44761 cttcactcac cctatagttt tccttgtctt cttctatgat tattccttct ttctcaggag
    44821 aacattcaat agcaaaccct gtctcatata gaacatattc tgtatctaat tctactttgt
    44881 gtacgcgaat ctgccagcct tcaaaaggaa taagccaagt tctgattttt gcctgtaaag
    44941 taggttccca tgtagaggca agtacctctt tcttacatac aatgtctcgt acattatttc
    45001 gaacgatgaa ctgtccatag gttttgatgc tctcattctt aaaactggtc gtgatatatg
    45061 tgtccgctcg ctgaatccct agcattgaat ccattgccat ttctgctttc aattgattcg
    45121 ctcgcggtat cgaaaaacca aattgactag aataagcaaa ttttaaatat ttttcattgg
    45181 tcaatgtatg gaaatttggc cctaattgtc ccgcattcaa aaaaaatgta tgtccattat
    45241 catgttgtat gagcaaattg gcttctgaaa gaagtttagt tccttcacga ataggcatag
    45301 gcatttcttt agccgtccaa tactcatggt cttcaggtag cgccaacaat aaaaaaatct
    45361 tattcagcca taatggagat aacgtactat tatagggttc agtcaatgtc aattgagggt
    45421 aggtataacc aatattcagg ataccttttt catcaaatat ttctttttgc atccaccatc
    45481 ttatattccg attgactaac cccttgattt cacctaattt aaaaggcaaa agatcggccc
    45541 acaacatagc tgaccaaaag cttgaaactg caaaacgata aatcatgctt cgaccaaaag
    45601 gcacatttgc accatcttcc gcaaaaaaat gaatatagtc tctagaaaaa tcaatagctc
    45661 ggttcttata tatctggcta atgggctttt ctggttctag tacgctataa atcaaaccat
    45721 aataatgaaa tgcaaaagga ttataataat cttggcgacc tcttgaactg tctcgatacc
    45781 agccattttg aagataacaa ttttctatat tatcaaatgc ttctttcagt gctgattctt
    45841 taactggata accaaatttt tggataattg ttccgacaat tatcttaaaa aattgccaat
    45901 taccatctgc acattcatat tcaaaaattt gcgagaacca atgaataatc tggctttttt
    45961 ctcctgtatc taattttttc cataataatt gttcatgatg atataacgct aataacattg
    46021 cgggcatttc taccatacgc tggtctcttt catgtatttc tccccaataa tcgggatggg
    46081 aaggatctgt tccagctttt attcctgata gaattaattt caaccatcgt tcatctaatt
    46141 ggtcaattgc gggccccgct ccccataaaa cccgagagaa tccttccatt cctgcaattt
    46201 tatttccata tcctacccct tgatgtatat aatcaatttg tgcgccactt gctcgataaa
    46261 acggctggag tggttgattt atctttttaa ataagtcaaa gtaatcctgt ctggtcctta
    46321 atagtggtac tggatgtttc attgtatttt ctcctctttt tactaccaaa tagctaattc
    46381 cgactgttct aatcgaagta agccttcaat aaaaaaataa tcagaataga tcagtggcac
    46441 attataagtc agttctctat ggtatgcttc tgctccgcct ccaatgatcc catcttcatc
    46501 catattccaa tttgcatagt gcttttctgt cttttggagt atattgatag cagcttcatg
    46561 atacattttt gatcgtacag aggaaacata tcctgctaat tccagaagac cgcatgcagc
    46621 gcatactcca gcactggtat caatattcga atcttttaca gcaaaatcaa tgagaggaat
    46681 ataaccagtt ttttcgattt tctgaataaa atttactgct atcttttctg ctatagctag
    46741 atagtacgga ttttttgtgt aacgataact taacgcgaat ccatataaag cccatgcatt
    46801 tcctctgctc caacaagaat tctcattgta tccttgcccg ccaagtaacc gaatatattt
    46861 ccctgattct gcatcaaatt ctgcgatatg cccggttgat ccatctttcc tcactagata
    46921 cttagctgtt gtatctgcat gctgccgagc aattttagag aacctcggat caccagtttc
    46981 cctcgaagcc caatacagta atggtaaatt catcatacta tcaataatca tccaaccagg
    47041 tttatggtca ttccatgcta ctaaatagtt ccctgaaata ttatagcgtc cagcaagtac
    47101 cgtagctgca tgtaacccta gttgtcgact atgtttgttt tgagactgtt gataatgtgc
    47161 aatagccgat ggtaagaata aaaaacctac atcatgatga acgtctgaaa aatggtacag
    47221 ggcagattct aataattgtt cttgttgtat tgctgcctct ttgaactttt ctcgttttgt
    47281 cttgtgaaag agctgccata aaataccact ccaaaaacca ttggtccacc agtcgattcc
    47341 tctgtccttt attacgtctg tatattttcc gttgtaagaa atatatggaa ttttattccc
    47401 cattctgtct aattccgcat ctatcttttc agaagctaca ttcaaaattt ttcttgacca
    47461 ttccttcgtc tgttcattca tggatatccc gccattcttt taatttttat gaatgtggag
    47521 ttgctcctag aacgaactag atggttaaca ttcgttattt tttttactta tttgcagctc
    47581 caatgtcatt ctccttaaat ttagactgcg cccacacata aatcaagacc attgggatca
    47641 ctgaagttgc taaaattagc ataattgcgc cataattaat tggctcacct gtactagaaa
    47701 ccgtcatatt tttgataatt aagggaaccg tatatttatc tttatctgaa agcatgacta
    47761 atggaaatag atattgattc caacttccca taaagataag taaaccacta gtaataatcg
    47821 taggtgataa aatcggtaca gcgattttcg taaaaatcac ccactcattg gcaccatcga
    47881 ttctggcagc ctccatcagt gctgtaggaa attgactgct atattgtcgc atcataaaca
    47941 ctgaagttgt actggcaatc gaagggatga tcacacctgc ataagtattc gataatccta
    48001 aacgactcat aatgtaaaat aaaggaatca atgtggtaaa aaaaggcacc atcctagata
    48061 gcataactgt attgaacaac agtttattcc ccataaaacg atactttgct aatgcataac
    48121 ccgccattgt agaactggct gtagctaaaa ctgtactgat tacagcaata acaatcgaat
    48181 tccaaataat caagggataa ttatatctcg ccatgatggc ttgccagttt tttaatagca
    48241 tatccccaaa cttgaaattg attccgccac ttaaaatttc ttgattactt tgcgttgaag
    48301 ctacaatcat atataaatat ggcatcaaaa atattaaggc accaaacaca ataatgacgt
    48361 acgtaaaaat tctgctttgt atcttttgtt tagtcatttt tatcacctcc gaatttaaat
    48421 tgaatagccg ataatataac agatacaaac accatcgtcc aggcaatagc agaggctttt
    48481 ccaaaatcaa ccatttcaaa acttgtttta tataaattga ctcctacgac caaagctgaa
    48541 ttttctggtc cattggaagt cccaaagaat atatttggga tattgaaatt acccaagcca
    48601 ttgatagtgg ctaaaactgt ggccattaaa atgattggct ttagcatggg gatcgttaat
    48661 aaaaagaatt gccgaaattt cgatgctcca tctatttcag cactttcgta tatctcttta
    48721 gaaatccctt gaagaccagc taaaaataaa atagtgttat atcctacact aagccaaatc
    48781 atcgtgatga tcattgatat tcgtatccaa aaaggatttg ttaaccactg tacaccgtta
    48841 attcctagcg cattcaaaaa tccattgaat aacccagtcg gattgaaaaa agtcatgaat
    48901 acggaagcta cagccaccgt tgatgtaatg gttggaatat aatagacagt tctaaaaaat
    48961 ccttttcctt ttaactgtgc tgaattcaat aaaacagcaa agtaaatagc taatagcatc
    49021 gttattggaa ttgatcctgc catgataatc aaaacattcg tgtaagattt aataaacaat
    49081 ggatctcgga agatagctag ataattgtca aatccaacaa attcaaatga gtagccaatt
    49141 ttcataaaac taaaagcaaa cgagataatg atgggaatca aaatcgtcac aataaagaaa
    49201 ataacaaaag gtgtgaccaa tccataagca ctttttttat gagacgtatc tgttgctttt
    49261 ttcttgctgt tctttattgt cttgcttggt aacgaagatt gcatcatcgt taagtcctcc
    49321 taatattttt taatcaccag ttaattttac gccgtactta tgagcaaaat tttctgcttc
    49381 ttttagatat gaattttcaa aatcacctgt ttgccaatag ttatatgcag cggtagtcaa
    49441 ataagacgta gcatctgaat aagtggctgg taaaacagta tttgatgtat tttcattgat
    49501 tttattcaat agttcatagt atttttgtcc tccttagtag tcaaactttt tctcagctgc
    49561 ttctgtttga taggccaatg agttagatgt agccacgccc agctccagtg cgttggctaa
    49621 gcattcttca tctgtcagca taaatgtaag aaattgttgt gccacttgtg gatttttggc
    49681 atctttaggg acataaaaac ttgatcctcc tgatactgca ccttttcctg gattttcttt
    49741 tgaaaaagga atcagtggag ccatgcgcca tttgtcagat gcatcaggaa aattcaaaga
    49801 catattcgta gcataccagc cgccagcaac aaacattgca gattcttgaa atgctttctc
    49861 agcatcttgt gcaccgtaga aatgtacaat atctgagtcg atcatttctt tgattaactt
    49921 tgcagcatgt ttcgcttctt tagtattcag acggatattt ccttcttcat caagcaaaga
    49981 ttgattctgc tgggctagca tgttgataaa caattcttgg tccccagatt ctggcaaggc
    50041 aattccaaaa ctaccagttt tttcttttaa tgcttttgcc acctggatat attcttccca
    50101 agaactgatt tcttcaaaat ctacacctat ttcagcaaat ttttttggtt gataataaca
    50161 aagtgctggg gtaaaatcac tagcccaagg aataatcgtt tcattactcg tttgttgacg
    50221 caaaatattc aatttttgat cataaaactg atcgccatat ttgtcataaa atccatattc
    50281 atcagcacta taaaaagcat cttgaaattt ttccagcaca cctgctgctg aagtgtctgc
    50341 catggagata atatccggca tatcttcgtg tgttaccaat tttggggtga tcatttgtac
    50401 tgttgctgtt cccgaagcta cattcacaat ttttaagtca atatttgctc cagttttgcg
    50461 gttatagatt tttgcagcat tttcaacata gtttgtcagt ttgccgtttg cgacatatac
    50521 ttctaatgta gtggcttttt ttatcattgg atctgcgatg tttgctgtta cttcttcgtt
    50581 agtttttcca catgcactta agctgaataa tagggtaatg tttagcaaag ctatcaccaa
    50641 tttgtatctt tttttcatgt ctatcccttc tttcttttca agttcttata taaaacgctt
    50701 tcacttatgt atattaacgg a
//
LOCUS       DAIGOK010000005        45652 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.005, whole genome shotgun sequence.
ACCESSION   DAIGOK010000005 DAIGOK010000000
VERSION     DAIGOK010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.005"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(450..1760)
                     /locus_tag="NOA01_000203"
     CDS             complement(450..1760)
                     /locus_tag="NOA01_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HCI0631439.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1772..3190)
                     /locus_tag="NOA01_000204"
     CDS             complement(1772..3190)
                     /locus_tag="NOA01_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCI0631440.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3430..4998)
                     /locus_tag="NOA01_000205"
     CDS             complement(3430..4998)
                     /locus_tag="NOA01_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HCI0631441.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5002..5412)
                     /locus_tag="NOA01_000206"
     CDS             complement(5002..5412)
                     /locus_tag="NOA01_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCI0631442.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5409..6332)
                     /locus_tag="NOA01_000207"
     CDS             complement(5409..6332)
                     /locus_tag="NOA01_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HCI0631443.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6329..7207)
                     /locus_tag="NOA01_000208"
     CDS             complement(6329..7207)
                     /locus_tag="NOA01_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HCI0631444.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7194..8530)
                     /locus_tag="NOA01_000209"
                     /pseudo
     CDS             complement(7194..8530)
                     /locus_tag="NOA01_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
     gene            complement(8533..9636)
                     /locus_tag="NOA01_000210"
     CDS             complement(8533..9636)
                     /locus_tag="NOA01_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HCI0631445.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9710..11218)
                     /locus_tag="NOA01_000211"
     CDS             complement(9710..11218)
                     /locus_tag="NOA01_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCI0631446.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11184..11876)
                     /locus_tag="NOA01_000212"
     CDS             complement(11184..11876)
                     /locus_tag="NOA01_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCI0631447.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11898..12872)
                     /locus_tag="NOA01_000213"
     CDS             complement(11898..12872)
                     /locus_tag="NOA01_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCI0631448.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13172..14044
                     /locus_tag="NOA01_000214"
     CDS             13172..14044
                     /locus_tag="NOA01_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCI0631449.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14502..16700)
                     /locus_tag="NOA01_000215"
     CDS             complement(14502..16700)
                     /locus_tag="NOA01_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCI0631450.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16932..17924
                     /locus_tag="NOA01_000216"
     CDS             16932..17924
                     /locus_tag="NOA01_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCI0631451.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17984..19405
                     /locus_tag="NOA01_000217"
     CDS             17984..19405
                     /locus_tag="NOA01_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCI0631452.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19398..20897
                     /locus_tag="NOA01_000218"
     CDS             19398..20897
                     /locus_tag="NOA01_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCI0631453.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20936..22330
                     /gene="uxaC"
                     /locus_tag="NOA01_000219"
     CDS             20936..22330
                     /gene="uxaC"
                     /locus_tag="NOA01_000219"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCI0631454.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22489..23172
                     /locus_tag="NOA01_000220"
     CDS             22489..23172
                     /locus_tag="NOA01_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HCI0631455.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23174..24010
                     /locus_tag="NOA01_000221"
     CDS             23174..24010
                     /locus_tag="NOA01_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCI0631456.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24021..25775
                     /locus_tag="NOA01_000222"
     CDS             24021..25775
                     /locus_tag="NOA01_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0631457.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26051..26392)
                     /locus_tag="NOA01_000223"
     CDS             complement(26051..26392)
                     /locus_tag="NOA01_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCI0631458.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26460..28631)
                     /locus_tag="NOA01_000224"
     CDS             complement(26460..28631)
                     /locus_tag="NOA01_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HCI0631459.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28800..29699
                     /locus_tag="NOA01_000225"
     CDS             28800..29699
                     /locus_tag="NOA01_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCI0631460.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(29770..30627)
                     /locus_tag="NOA01_000226"
     CDS             complement(29770..30627)
                     /locus_tag="NOA01_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCI0631461.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30612..33314)
                     /locus_tag="NOA01_000227"
     CDS             complement(30612..33314)
                     /locus_tag="NOA01_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCI0631462.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33327..34616)
                     /locus_tag="NOA01_000228"
     CDS             complement(33327..34616)
                     /locus_tag="NOA01_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HCI0631463.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34766..35812
                     /locus_tag="NOA01_000229"
     CDS             34766..35812
                     /locus_tag="NOA01_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCI0631464.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35814..37967
                     /locus_tag="NOA01_000230"
     CDS             35814..37967
                     /locus_tag="NOA01_000230"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HCI0631465.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(38440..39891)
                     /locus_tag="NOA01_000231"
     CDS             complement(38440..39891)
                     /locus_tag="NOA01_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCI0631466.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(39888..41612)
                     /locus_tag="NOA01_000232"
     CDS             complement(39888..41612)
                     /locus_tag="NOA01_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCI0631467.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(41605..42219)
                     /locus_tag="NOA01_000233"
     CDS             complement(41605..42219)
                     /locus_tag="NOA01_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCI0631468.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(42564..44021)
                     /locus_tag="NOA01_000234"
     CDS             complement(42564..44021)
                     /locus_tag="NOA01_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCI0631469.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(44062..44982)
                     /locus_tag="NOA01_000235"
     CDS             complement(44062..44982)
                     /locus_tag="NOA01_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCI0631470.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(44994..>45652)
                     /locus_tag="NOA01_000236"
     CDS             complement(44994..>45652)
                     /locus_tag="NOA01_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCI0631471.1"
                     /translation="NLLISVVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVY
                     AFLSPDKGIFNQWITGHGGEAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGI
                     DPTYYEAAMVDGASKWQQIKHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSG
                     ALYDVTQVLDTYIYNGLTSTGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF
                     "
BASE COUNT        13449 a         9339 c         8037 g        14827 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt tcgatttcgt
      181 ttttcgaaat ctttcagcaa ctcatttatc ataacttatc gtttgttatt tgtcaagaac
      241 ttttttgaag tttttttcga tagaatcaag cttcgcaagt ggttcttttt aacgacttag
      301 ttatcttatc acgtctaaaa acatgtgtca actctttttt ggaattttca taaaatgttt
      361 caatgaagcg cgacaacgtt gtttaatata tcattaaaaa aaataaaggt caagctaaaa
      421 tattatttaa gcttgacctt attataattt caacgattgt caaaagtatt gtttgcagct
      481 gtgttgccag tttctataga taaatcacag cttttgaaac ataagttttg tactgcttcg
      541 attcgcccga attcttttgc agttatttgg caatgagaaa agcagacatc ttctataggc
      601 ttttcaggaa atgctactaa atcaaaagct cgggaaggaa gtgagtaacc tggagaaagg
      661 catgcctgta cgtttttgat gtgtatgtct ttgactttcg tcatttgctt tgattcaggt
      721 atttcttcag ccactgcttg ccaactttct ggcatttgag caaggtcatt gctatttttt
      781 ctattgtatt cattatgcca gtccattatc caagaaaaag gaaattgtac atcaatcata
      841 ttcagatttt ctaccctgat attttcgatc actccccctc tttcttttga agatttcatt
      901 cgaataccac agtctgactg aaaaaaatcg atatcgtgaa tatatacgtc actaactcca
      961 gcactaactt cgctaccaat agttacaccg tatcctgaac ggattttaca tctagcaacc
     1021 tcgattcttg atgattttcg attgactctt gcaccattgc catctctgcc agacttaata
     1081 gcaatacaat catcaccaca ggccaaatca cattcataga cacgcacatt tgttgaagaa
     1141 tcaatatcaa tcccatctgt acttggtcca tcattgttgc ggatgattac tttttctact
     1201 aatacatcgt tagaataagt gatttgcaag ttccaaaaac ctgatttctg aaaaatcaaa
     1261 tcacgaacaa caacatgttc tgcatgatac agtaaacaag cgcgtggacg tttaatagca
     1321 taatccgcaa tccaacgaag tcccttttga tcgtattcag ctcttgtccc gcttttttga
     1381 tcttttcccc aatacagctc ccaccaatgg tctccttgac cgtcgattat tccttttcct
     1441 tctataagaa tatctttcgc cgagagaaca ttcaaaatcg ctgctggcca tttcatttct
     1501 acgccggcta ctcgtgtatc gatttctgga aatgcttcga tatctgttct ccccttgagg
     1561 actgcacctt cttcaaagat aagatgactc ttatctttta aaaaaagggc tccgactaaa
     1621 taaatgcctt ttggaataaa tacaggtaag tgttctttcg caccttgatc gattgctttt
     1681 tgcagtatta ttgtatcatc ttgttgagtg tcgtttgttt taaagttcaa agcattgata
     1741 atcccttttt ttgtccgcaa tacgtttctc cctatctttc attgattttt ttcaattctt
     1801 caccaataaa tacagttttt gaacgattca gtgggtaacc tactagcaaa atgattgcgg
     1861 atacaaataa gcagaatgct ggtaacaagt ttgccatcgt ataaatcctt tcttgtacta
     1921 aaactgattg gatggctcca cctgttgtag acgattggta accaatgagt gccagcataa
     1981 atccgcctac cgctcctgca catgcttgtc ctacttttct cgcaaaagaa tttactccat
     2041 atactgttcc atcttctctt aagcctgtct tatattgatg ataatcaatc acgtcagtaa
     2101 tgaatgccca gaccatcaaa ttgaataaac cagatcctaa agtagcgata aataaaagaa
     2161 tcagatatac ccatgcattt gttatatgaa ggaaatacat cagcaagtac atacatgaag
     2221 aagcaagtaa cgcaaatacg cttgcttcct ttttaccaaa taattttgtc agctttgtag
     2281 caaatggtgc taaaactact actgttccgt aagtaaataa aagagcaaca gacattgctt
     2341 gtttattatg gaagtaatca ttgaataaat aagtcatatt cgtcccagct aaaatctgat
     2401 tgatcacaat gaaaatgtca acaatgacta aaataatcag tgctcgatta gctaacaaac
     2461 cttttaccaa ctgagaagca gatacttttt cgtttttgac aactcttact ctttcagtag
     2521 tcaatttaca agttaacata tatgcgacaa atgctattgc agcacatccg attgaaatcc
     2581 aaaagaaatg attccctgaa acgacttgtt gtccactctt gttatgggta tacattaaaa
     2641 tcggaatgaa gaatcctgta gttgatccgc caatcgcaga accaatgctt cggaacgtgg
     2701 acaacgaggt gcggtcatta ggatttgagc taattgctgc tgccattgct ccatacggga
     2761 tattgattgt tgagagaaaa atcccataaa caatgtatga aacaaaaata taagcaatct
     2821 tcaccgtcat tggaagatgt tggacgaatg gcaaaaacaa aatgacagtc actaagcaaa
     2881 atggatactt catttttctg atccaaggaa taaatcggcc agtttctgtt aactctgaga
     2941 catcacataa tcttccaacc atcacatcag caaacgcatc caaaattctc gaaatcaaga
     3001 ataacgtacc tacgattgcc cctgaaacgc caagaacatt cgtataataa atcatcaaga
     3061 aactattgac taaacctaaa ataaaacagt tacctagatc accgaacatg tagcctatct
     3121 tatcggaaat cccaaattcc tttttttctt tttttattgt atttgctgta acagcatttt
     3181 gtgaaagcat ttgaattcct cctacctctt ctagtaaacg ttttcaaaat tataactctt
     3241 tttttcttgt ttttatattc acaatgttgg ctttttattg cattatcttg acagataaaa
     3301 aacaaaaaat aatctaaaaa cattgatgca taagattgat aacttttttt acataacaat
     3361 atatacaaac aaaaaaattc actaactagt gaaaataagt tagtgaacct catttattaa
     3421 agaattaaat tacagatgat cttttccatc atacttgaaa aactcaaaat cagcataact
     3481 tcctcttttt ttatttagat catgcactgc tatcccgata aaatttccag taaaccctcc
     3541 tgagagtata cacatgtttt tttgcggacc tactttcttc cattcttcag ttgatacgtc
     3601 tttcaaatga aactgtgctg tctctttctt tacagtgact ttcaaaaaag tcttcttttc
     3661 tgtgagtgga atcgtccaat cttctatttg tgctactccg ttttctgatt ctatcatacg
     3721 taggcatttt cccttgtcct cattccaagt aacataacaa taaatataat tggattcatt
     3781 taagtacagt accagtccag ccatctgatt aaaattatta ggcatgtatt caaatgctgt
     3841 ctccgcatca aaagtaaaat ccgtttgccg aatagctaac aaatgatgtt cgaaggtaga
     3901 ttgaagagat tcaccggaaa tcagacgtaa aaaacctggt ctacattttg tatcacacca
     3961 atcagcattt ggcaaaatgc gtctggcatt ccagttcttc tttagctctc cttcaaatgt
     4021 atccataaac tgatgatctt tttcttgttg tacctcttca tttgtctcaa tcattatttc
     4081 ttccatcgga gttgtctggc tatctttaag tcttaaccaa tcgtcggacg tccaatacac
     4141 ttcttgaata gcggtttctc ttcccaaaat agccgcaccg tttaatggtc tggtacaaag
     4201 atatgtcatg taccatcttc ctgacggggt ttgtactaag ctcccatgtc ctgtacattg
     4261 caatgtagaa gcaggtttgt cactagctgt cagcatagga aaaccaggat caagctcgta
     4321 aggaccagta atattctttg ctcggcaaac tgtaactgaa tgtcctcttc ctgtaccgcc
     4381 ttctgccgtc atcaaataat aatacccgtt acgaaaatac aaatgtggtg cctccgtttt
     4441 cgctaattct gtacctgcaa atattttata gatgggacca tcaagcttct tcttctctgc
     4501 atcatattct tgtaatacga taccgctgga tttattcggt gtgtccagac gataatccca
     4561 tagttcattg acaagccatt tttttcccgt ttcaagatca tggaacaaag aaggatcaaa
     4621 gccgttacta tttaaataaa ttggttcgga ccaaggacct gtgatctcag atgcagtgat
     4681 caaataattg tggacatctt taaacggacg gcctgcactt ttgacatctg tgtaaacaag
     4741 ataaaacaat ccatcagcat aactgatctg tggcgcccat atacttccat ttgtaggatt
     4801 tccttgcaaa gatacttgag tggtcaaaat atccgttgta tgtgaccagt tgactaaatt
     4861 tttcgactca tatactcgga ttcctggaag ccattcaaaa gaggagacaa taatgtaata
     4921 ccgctctttc actcttaata tattaggatc tggattgaat cctcttaaaa taggattttt
     4981 cactttgata gtctgcatga ttcaggctcc ttttctcatt cttactcgat ataaactgaa
     5041 tgccccagct gcgaaataaa aaaatgcaaa aggaaacgca ataaactgag tgccaatcaa
     5101 caatatgaat cctgttataa aatacaaacc agtggcagtt gttaagtatt ttcctttagc
     5161 agtcaaaaac aatgctactg ctaaaaaagc aagtgaaata aacaacacaa cggcaaacag
     5221 accagtcatg tcctgtaata atccaaatgt attttctaaa gacaaagttg aatgatctac
     5281 agcaagcgac tgataacttt ttttaaactc tgacgcagac atattactta aagtaactgc
     5341 taacccgcca aagaaaatca aagatagact gccaccgacc attcctagca atagttccca
     5401 ttttcttttc atttctgaac cccctgcttt tctaaatgta tccattcagg tcttttgatc
     5461 gatgtttcat aattgttatt acgttcagag aatttgacag tctgttcatt tttcttgtca
     5521 tcccaattat cccaaccagt tgggtgaaca tgcccaccta tcttacacgc ttcaaataag
     5581 acatttgcat acggacgcca cggtcttcct aaatagacgt ttctagtatc aggttctgca
     5641 gtcaaggtac agctttcaaa acgataccct gttttttgat ttttcggcgt agatgctgct
     5701 gtgatgtatc caggattttt actggctaag ctatggatat gacactgatg aaaaacggca
     5761 tctgccccac caaatatgaa atccaccgtt ccttcaatcc agcaatattc ataggtctga
     5821 cgacaatact caggattggc agaagccggt gtacaaaaag gagtaccatc tttttgcaaa
     5881 tcaggcaacg gacccgtgca taaggtatcc tgataaccag atagtcgaca gttttttaaa
     5941 gatacgcgat ctcctgaatt gaataaagca acagcctgtc ctaccttttc tcctggacct
     6001 gctgtatttt cgatgtgaag attttctaat acgacattcg atccttctaa gaaaaaagta
     6061 gcacttcgga aagtccctaa ctggctaccg tcaagatact cctgttttgc aaaacgatta
     6121 ccggagataa ctactgttcc tgctcccaca agtttaaaat tggataatct gctttctata
     6181 tattcatcat atcttccttc ttttactatc aagacctttg gatggctatc cggcaggcct
     6241 gccaaataat cgattccttc ttggatagtt tcaaaatccg tcggggtatc tatagcattc
     6301 tttccaatta tcagtttgtc tttcatcctc ataaatgctc ccttatgaat tgtttccaaa
     6361 tatttctcat ttcttgtgtt tccatatgac cgcctgttaa agaaaaagaa gaaaagtttt
     6421 ctggactacc cattgattga tactttttgt ccagcccttt tctcagcttc agtacgcctt
     6481 cgtccaagca catcgtatcg ttttttccaa ctaagctaag acgagggcgc ggagcgattt
     6541 tttcttggat tgctaacgtt gtataagctg tcaatagatt tggcacatag taataaaacc
     6601 catgatgatc taggcggcga tgtagcaaaa gtatttcgat gtccacctgt gccgctagat
     6661 caatacaaac tttgacacga gaatccaatg ctgtcaccca ccagcttaat aagcctccca
     6721 tagaaagacc aatcgttgcg agccgttgag tatctgtgtc ttcccttgtc tccaaataat
     6781 cgatcaagct gatcacatca taaatacgca ttccccataa cgtttttcct gttaataaaa
     6841 attgtttgaa caactcgctt tcagaaatac ctccgcgttc ttcaaatccc caagcatcaa
     6901 tcgaccaaac agcatatccc attcctgtca gtacttctgc aaaagatgga gaagctaagt
     6961 aaggagcacc atggatcaat tcacttttcc cgactgaaaa atctccgccg tgagaatgca
     7021 ggtaaaggac cggcggaaac ggtccttctt tttctggata agcatagagg gctggtactg
     7081 tttccaattc atttaagtac aattgatagt attcaagcat aaaaccgttt tgtttttctc
     7141 ggtataatcg ttttccacta ggggtatgtc tgctagatgg agcacctaaa aaatcattca
     7201 actgcataag caaagttttt acgaacaacc agttcatcca tattttctga ttgatttgat
     7261 tggaatttct caatactgat ttctttagta ttttctaata gaaatgctgg tccctcgtga
     7321 ttttcaatcg acatctggtt aaaggaaacc ttacccgcaa agccaatata aaatccttgt
     7381 ctgctcattt cctctattcc tgtcatcata gcaggaacac cgggaactgc atcttgggcc
     7441 atcgagacat cgatattgtc aaatgtgatt tcttgtatga attgttccgc taatccataa
     7501 ataaaacctg cagaagcatg gacattcctt gccgtgatat tcgaaaaatg gatccttctg
     7561 aatgctggtg tccgttcatc tatcggataa gccttttttc ccatacatac ggttctttcc
     7621 ctcgcggacc acagaaataa taaagattca gtataaatgg acacatcaca ttatccatga
     7681 ctatattgga tactcgaata tcttcaacga tcccgcctcg acctcgtcta gatttcaatc
     7741 gtattcctcg atcagtttct tggaagatac aatttgaaat ggtaatatta cgaatactac
     7801 cactcatttc acttcctaat acgacaccgc catgtccatg gaccatcgtg caatttgtga
     7861 tcgtgatatt ttcgcaagcg atcctttcgt acgtatcttc agtccctgcc ttgattgcaa
     7921 tacagtcatc tcccacgtca atatggcaat tactaatacg cacatttttg catgattctg
     7981 gatcaattcc gtctgtattt ggtgaatccg ctgggttcaa gatcgtcaag ttatcaaacg
     8041 ttgcattact gcaaagaatc gggttgatcg tccaacttgg tgactggatc aatttaatat
     8101 ctttcacagt aattcgatga cagttatgga aactcatcaa tttaggacga ggataagcga
     8161 gattatctgg ttcatttcta aaagtatgcc accatttttt tccatttcca tctaatgtac
     8221 cgaaaccagt gacagagatg ttctctacat tttgagcata gatacaggaa gcataaactt
     8281 ttcgatggac tccttcccaa cgtgaatgaa caacaggata atcttttgga tcgtcagaga
     8341 atttcagaac cgctcctgcg cttaaatgaa gttcgacatt gctttttaaa aataaagctc
     8401 ctgttagaaa ttcacctgct ggaacaacta cggtcccgcc tccgtcactt gctgcagcat
     8461 caatggcttg ttgtattgct tcagtattca attcatcaat tgatgcgcca aatgtcaaaa
     8521 tatcatacat atttataatg cctccacttc aatcaatgct tgcaagaatg caccaacgcc
     8581 tttttgatca ttacaaataa ttggttcact gatgtagtaa gcatatgaac catctcgctg
     8641 atctgctccg cctaaccctg ctacttgaca gtttttattc aagttcagcc agccttcttt
     8701 tgtttccaga acaaattcat caagtaatcc ttgatgtgat ttcttcactt gtggaagcca
     8761 ttcttctttg ttcagcagtc ccaaacgtat tcctttagcc attgcgtaaa caaacatact
     8821 actgccggaa gcttccagat aatttccttt ccgttttgtt tctgttgtta cttgatacca
     8881 tgtcccgctg ttttcttctt gatatggaag taatgcagcc agtgtctttt ctagaatttc
     8941 tttcagcaaa gaacaatctg tttcttcatg aagtaattca taggtatctg ctgcagccat
     9001 caaataccag ccaatcgatc ggctccaaaa atgatgggat agacctgttt ttggatcagc
     9061 ccatggttgg accttctttt catcccatgc atgaatcagc aaatttgttt ttggatcgaa
     9121 tagatgttta taactgattt caaactgtct caaaacatct tggtaatctt tttcttcact
     9181 aaaattctgg ataaattcag cataaaaagg agaacccata tataagccat ccaaccaaat
     9241 ttgataagga taaatttctt tatgccaaaa agctccttcg gatgtgcgtg gatggtttgc
     9301 caactgctgg gaaaggagtt ctgctgcttt tttatatttt tcttcgcctg tttctttgta
     9361 caataaaaag aaaagctttc ctgtatttac atggtcgata ttgtattctt caacgctgta
     9421 gccacggatc gttccatctt cttgcacgaa atagtcgata ttgtctttta tgaaatcaaa
     9481 ataaattttt tcttgggttc gttcccaaag taattggaac cctttaagta cgacgccata
     9541 gtcataagac cattttcctt tataccactt ttcttcatat aagcgaggtg ttctttccat
     9601 gatacttttt gctaatttct ttgaccagtt ttccattatt tgctctcctt taaaaattaa
     9661 aataggattt gggacaaata ccaagaagta tttgtcccaa tcgtctatat tactgtgccg
     9721 ctgctttgta tgcttcctcg tactcttgtg tgattcgatc gcctccttgt gctttccagt
     9781 cttctacagc ttttttgaat ccatcttcgt caagctgtcc catgatgtat tgcaccgtag
     9841 catcaatgac gattttttca atttctgatc ctacttcatt gttcgtcggt gaatccaatg
     9901 gcacagaagg atccattaca gcaaattctt cattttcttt gatcaattga ttagagaatt
     9961 ctttttcagg gtctgtatct ttgaaagaga acgtcacttc actaggacga gagcttgata
    10021 acggttgcac ctcttgttgc caaagatcca tatcgataaa ttcgatagaa ccatcatcat
    10081 tcaattcata atgcgttcct tcgataccgc cagtcatcaa tttatacaca tcttcttcta
    10141 aaagatcatc aacaaattga agtaatttct tcaattgtgc ttcgtctttt acttgagatt
    10201 tgggaaatgc tagcaagccg ccaataccat ttccttctga ccaaacatga taaccgccgc
    10261 cttcaccatc tgagatcttg tttacaggca ctaattccat atcatcttga ataccttgac
    10321 ccatcgtctt cagattcttg atatccacca ttccagtgta gattccagtt cttccttgtg
    10381 caaattgttc ttgctgatca gtcttttggg taactgcaaa gtcttgtgcc aaatagccat
    10441 tctcaaatag atctcttgaa aaattcaaag ctttcatata ctcttctgta tcaaattctg
    10501 gagtgaattt gccattatcg tctacagccc agccatttgg tgctccaaaa taagaagtga
    10561 aaagtttaaa gctggtataa cgaatatcac taggcgcacg atcgccaaaa ccaactgtgt
    10621 cgtccacacc atttccatca ggatcgtttt ctgtaaattg tttagctact tcataaagct
    10681 catccatggt agttggaaca tctagtccta gattatccaa ccagtctttt cgaatgacta
    10741 agccagcacg agcgtagtct ttttgtatcg gtacgccgta caaggcaccg tcgatactcg
    10801 cagcttcgat tctatcttca gaaatttgac ttaaattttt atagtctttt aaatattctg
    10861 atacatccca gaatacgcct gattttaacg cattgcggac agaagagttt ttcaacatcg
    10921 caatttgaag tgtaacgata tctcctagtt catcagaagc caatgcagtt gttaatcttt
    10981 cttcttttga agcatcagga atccagttga atgtcagttc cgtatcggtt ttttcttcga
    11041 tcaagttcac aatcgtgtct gtaggcggtg aagcatgatg caaaatattt aaccaagtga
    11101 ttttagcttt tccatcttct gtctctgaag aactgttact tgaactgctg ccgcatgcgg
    11161 caagtgctag aattcctgca aaactaagac ctaattttac gagcgttgct tttttcataa
    11221 attttctcct tcactgtttt aataatattt ttgttttctt ctgttttttc tgtatatgag
    11281 ttcttccatt ccaaatggca aaatatagaa tttgctgtcc acgtgactgc ggaaaagaag
    11341 agactgccac cgataaaggt taaaacacct ggcattgttt ggatgatcat cagataaatc
    11401 cctaccatac ttaatatcaa cactaatgag taatgaagat gtgagaatcc gaataaaaat
    11461 gccattttca ttttgaaaaa atatcctttc caatcataat gagccatgat tgggaaaata
    11521 tagagtgctg agagcacata tatgaacgca gcaatataca aagcgatcct aagataccaa
    11581 ttgtgtatga aagaaagatc cacaagtaaa acaatcccga tggcgccata tatccagctc
    11641 atcagcaagg tttcacgata gttttcttta aaatatgtga agtacgtttt cgtcaaagga
    11701 aacgtttctt ttgagcgcag ccattttcta atcactgcaa ccatcgcata cgatgcagga
    11761 ccgaaagtaa aaactcccaa acctgcgatg actaaaccgc cccacagaaa attcaaataa
    11821 accaactgta aaatacgatt tagccttccg tttattttat ctaatgactg cagcatatct
    11881 ttccctctct ttctatctta gtaaacgccg tcttctccta gctttttggc aatcttatta
    11941 gctaaaatta ccataaacaa tcctacgacc cctttaaaca atcccattgc tgtactgaaa
    12001 cttaactgtc cattcttcaa tccagctgta taaatatagg tgtcgaaaat ttctgcaacg
    12061 cttcgattca atgaattcaa taacaagtac atatgctcga accctaagtc taatgtctga
    12121 ccgattttta gaatcagcaa ggtaatgatt actggacgaa ttgccggcaa tgtaacatgc
    12181 catgttttgc gtaagcgtcc tgctccatcc atttctgcag cttcatacaa ctgtgtatcg
    12241 actgcagtta tcgctgaaag gtagatgatc gtagaccagc ccaactcttt ccagatcact
    12301 tgtccgatat atactgtgcg tgtccattca ggggatgtta gaaaactgat tttttcaaat
    12361 cccattgttg caaggaattc attcaatacc cctccgtcaa cattcaaaaa tacataagta
    12421 atcgaaacaa tgattaccca agacatgaag tgagggatgt aaatgatcgt ttgaatcccg
    12481 tttttcaatc gtttgttcgt tacttcattt aatagtaaag acaaaatgat tggcatcgga
    12541 aaaaacacga agatatttaa tccgaacaag atcaacgtat ttcttaataa tgtaaagaat
    12601 gtcggttctg taaacaaacg aatgaaatgt ttgaaaccga cccaaggact tcccattatt
    12661 cctaaaaatg gttgataatc ttgaaaagca ataacgagtc cccccatagg aagataccta
    12721 aaaatcaaga aataaaggac tcccggcaga atcatcacat ataaaaattt attcgttttg
    12781 attcctgcaa ttattttttt tcgtcttctg agcttttctt cttttttcga tagcgtcagt
    12841 tgaaatggtt cttgtttctt tacactttcc ataagcccct ccttagtttt gtgaatgttt
    12901 gctgcttatg acttcatgat agataactat ctgaattacg tatataatca tttttaccgt
    12961 aagtaaaaac gcttacatag caatgatttt tgtgctatac aaaacgataa agtgtatttt
    13021 ttcacaaata aactacgcat agcaaacctt ctattttttt ctatctgaca cttagcatag
    13081 gaaatgatta tatacgtaat ctttcaccga tgataaattg aacatataga aacagaaaca
    13141 tttaatttat tttgaggagg agcctatcta tatgaaaaag aaagtctcta taggagaaag
    13201 aagttttaca cttttcaaca atacgtttct aattttgcta gctttgatct gtatcgttcc
    13261 ttttttaaat atcatcgcta catcttttgc ttctacacaa gaagtcgtcg cgaaaaagtt
    13321 tatccttttt cctactacct tttctttaga cgcgtatcgc tacattcttt ctacgccgac
    13381 catttttaga gcacttgctg tttcgattgg tgttacaggt gtcggaacga tcgtcagtat
    13441 gtgtgcaact tcactgatgg cctacggtct ttccagaaag tatttgtttg gtcgaggttt
    13501 cgtcaacttt cttgttgtat tttctatgtt gttcagcggg gggatgattc caacgttctt
    13561 agttgttcgt tctctagggc tggtcaattc ttactgggcc atgatcctgc ctgtggctgt
    13621 taatgcaatg aatatgatca ttatgagaaa ctttttccaa gccttgccgg atagtttgga
    13681 agaatcagca aaaatggatg gttgcacaga ttttggtgta tttttcaaaa ttatgctgcc
    13741 attagcgtta ccgtctattg ctactatctc gcttttttat gctgttactt actggaacac
    13801 ttatatgact gctattttgt atatcaacga ttcatctaaa tggccgatac agattctttt
    13861 acgccaaatc gtcattgtat caagtgggat gcaagcagaa agtagtgccg ttgatgtgat
    13921 tccaccagca caaacagtta aaatggctgt tatcgttatt gcaacagtgc ctatgctgat
    13981 tgtttatcct tttgttcaga aatattttgt caaaggagca ctcgtaggtt ctgtcaaagg
    14041 gtgattatgt actgaacgca aaatggtagt tgtgtcagaa gtggtcagct tcaagaaata
    14101 agaaggcatt tacgaaaatt gcttcttaaa tttttgtgaa atttcggctt atttttcgaa
    14161 gaatctactc ctggaacacc gtgtatcaga ggttgtgaaa gagacgttca gccttgagcg
    14221 atgataaaaa ctctcacaaa cattgcgacg agtaacccac aggagcaata agaagcgatt
    14281 ttgaaaaata gcttcccata tttcacaaaa ttatgcctta ttgtcgatag cctgtctctt
    14341 gaacgccatt tattcgtaaa aaagaactgt gacaagactt ttgtcacagt tctttttgcg
    14401 tgcttagcct tgaaatttaa ctatccataa atacaaatac tagggttatt ctaacttctt
    14461 ttattgcttg aatcagtcca caagaaaaac agcctcttga ttcacaaata tttttttcga
    14521 tattctcccg gcgtaatcga ttccttcttt ttaaagaaac gaataaaatt ttgcggattt
    14581 cgatattgca atctttctgc aatctctttt acggagaggt tagtctcttt caaccatttt
    14641 tttgctacct ctaaacgatg attttgaaca aaatcaccaa atttttctcc tgtttccttt
    14701 ttaaatacat tacttaaata gttagggtta taatgcaacc tatcagcgat cacatctaaa
    14761 gacagatcac gatcatattc tgtgtagacg atatgcataa tattctccga caaattttta
    14821 aattcatgat cagtgattgc ttgagagcat tctgtgattg gcagaactaa ttgttctaaa
    14881 acaagttttt ctagttcttt gggatgataa ttgttcagtg cttttacata aatttgtttc
    14941 atgttatcaa agttttttga atctactccc aataactgtc ctaattgaac gagttcattg
    15001 atcaagcgga ccgaaactac ttcccgactc aaaggatttt tattttgtgc aaacaatgca
    15061 tcaatcaatt catgtactaa attcttcact tcctgatttc cagaacgaat ggcttcaaac
    15121 aatcgatttt gttcttctat cggatatcta ataagtgttt tttcatgagc agcaggaacg
    15181 atttcctgat aaaaaataat tgaacttgga ccagtattta cacgataata cagggcttct
    15241 tttgcccgat caaccgattg cttgctttca ttcagcgttt cgaaccggtt actgatgcct
    15301 atactgatag ttaaattcaa gtattctttt acacgttttt gaatgagtcg gcaatactcc
    15361 atgacttttt tgcttgcttc tgcatcatct ttatccattc gataaatagt ggcttgcatt
    15421 tcttcattca aaacgatcgg cagcattcga ttttcctttg gcacaatttc cgtgatcatg
    15481 ttgttgatcg ctaaaagtag caaatcacgc tccccaccgt gactatcttc taacagatcg
    15541 atttgaacca aagctgtata atagcagtca ttttgagaac tataaccaaa ttgctgcaac
    15601 cgctgatcta gttctcgcct ttctactcga tttctaaata aactaagtac aaatagcgtt
    15661 tccagctgtg gcttttgggt aactaagtgt gcacttaacg cttctttttc tcctatgatc
    15721 cgactgactg attgagcgac caaatctagt tctttcccac tgaatccttt tgatttaaga
    15781 ttcagacgtt cctgtatttg agaaatcgga cgggcaaatc tgtccgaaaa taagtaggaa
    15841 agaagaccaa tgagtcctac cagtgttaaa gacactgcaa ctagcccgat tctcagattg
    15901 cggatcattg cgccaattgc actagagtca acttctaatc tgtaaatcca attattataa
    15961 tctgatttca gtactatata cgttttatct ttttctctta tcactttcag ctgattgtaa
    16021 tctagatcca cgtccgttaa tacagttggt tgctctttat tttttactgt ctgagtagta
    16081 aaaaggttgc caacttcgtt ttcaatagaa agtatgtctt ctccactttc accaacaact
    16141 tgttctattg tatgtttctc tatcgtagca attcccaatg catatttttc tttttcaaac
    16201 attggcaaag aaatcaccat ttcgattcct ttgtttttct tttgccaaaa taaattattt
    16261 ttgttattta tgtacttctg ttgatacttt tcaatttcct cttcggtcag ttgactcaac
    16321 gatccattga ttactcccca tttaccagcc aaactgacta aatcataatt attatcatcc
    16381 ataacaatcg tttctataaa atttagttct ttcctgattt cagaataagt ggagtaatct
    16441 gtatatgtca gcggatgctt gaatatgtcc gagaaatttt tggtcgaacc atacgtagaa
    16501 aacgaatatt caatcgttct aattttccat tcaacgttag ttttaacttg cctgagcaaa
    16561 ttctttttgt ctgttaaata actcgattcg atcgattcat aggtattctg gtaaatgaat
    16621 aaactaaatc ctatgacaag tacggctcct aataaaaaca tcggtaaaaa tattttataa
    16681 aatactgtac ttttcttcat tttcttcctc tcctcctttt aaaataaaag gctttcattt
    16741 ttctgtaaaa ttgatactca tctaattaaa tgagcattcc ccgctttcaa gaatagcata
    16801 attaaaaata aaaatccaag taatttttaa cgtgtgcaat cttttcatat caaagatcta
    16861 ttgacttatc gtagttatcg taatatgatg aatgcgttaa caaaaagtga aaaagtaagg
    16921 aaaatgatgg aatggtaacg atcaaagatg tcgctaaagc agctggcgtt tctcatacga
    16981 cagtttctcg tgctttgaat aacagcagtt taataaaaaa tgaaacaaaa caaaaaatac
    17041 gacaactagc taaggaaatg aattattcgc caaactatag tgcaaaaggt ttagtgaatc
    17101 aaaaaaaata tctaatcgga ctatttttct caagtttgca taaaggaact tcatcaaatt
    17161 tcctagcaga ttccattgca ggcatccatt cagtattaga cacaaattac agtttatctg
    17221 ttgaaagtat cgatgttgtt gatttaacag aaatcaacct gcaacgctac gatggactga
    17281 tcgttatgag tcaaagtgat gatgatcagc ctttcatcga ctatctcaaa gaaaaagaat
    17341 ttcctttcgt tgttgtcaat cgtcacatca atgatccagc ggttatcaac gtcactgccg
    17401 atgatgcggc cggtgttatg caggcagtag attacggtat tggatgcgga catacgaaga
    17461 tcgcttatat tggcggaaag aaaaattttc gttcgacaaa agaacgggaa aaaggggtat
    17521 tgaaaagtct aaaaaaagcg agcatccctg tcaatccagc ctatttttta acaggagatt
    17581 acagcttaga aagcggcctc caaaacatgg aatacctatt atcactttct gacctgccta
    17641 ctctggtttt ttgcggcaac gacgacatag cgattggtgc attgagagct gtacatttag
    17701 ctggcctccg tgttccccag aacatttctc tgatcgggtt cgatgattct ccagtcgtct
    17761 cctatctcaa tccgccgctt accaccgtac gtaagccttt gaaagaaatg tgtcaagaag
    17821 gaacaaagct tttactagat ttgatcgaag gcaggacgat cgaacaatct aaaattgagc
    17881 tctccacctt gttgatcctt cgtgagtcga tcaattactt gtaaactaca agtaatttta
    17941 tataaaaatg ttaacgtgtg cataacacag aaaggaagtt tatatgaaaa aactgacaaa
    18001 agaattagtg aaaaaaaacg aagcacctgt aaaagttctt cagtttggtg aaggcaattt
    18061 tatgcgtgga tttattgatt ggcagattca gcagctgaat aaccaacaat tgtttaaagg
    18121 taatgttgca atcattcaac ctttagaaca aggtcttgga aatatgatga accagcaaga
    18181 taatttatat acggtcattt tacaaggact cagcaatggc gaaatcgttg atacttccga
    18241 gattatcacc gtagtagaac aaatgatcaa tccttatgat gaatgggatg cttatctatc
    18301 tctagcagaa aatgacgact tggcctatat cgtctccaat acaacagagg caggaatcca
    18361 attcgtggag actgataatt taacagatac tcccccacag agcctcccgg gcaaactgac
    18421 agccttttta taccgacgtt tcaaattaaa caaagctggc tttaccatca ttccttgcga
    18481 actgattgac agaaacggcg aacgtctaaa agaaatcatt ttacgctacg cagaaaaatg
    18541 ggatttagaa cctgcttttg ttgagtggtt agaaaaagaa aatgttttct gttgtagttt
    18601 agttgatcgc atcgtcccag gttatcctcg cgaagatgca ggtgttttca atcaacggca
    18661 taactatgaa gatcaattga tggtcaaagc agaaccattc atgctttggg tgatcgaagg
    18721 gcctaaagaa ttggagaatc aactaccatt caaacaggct ggcttgaatg tcatcatcac
    18781 agatgatatg actccttatc gtgaacgtaa ggtgcatttg cttaatggac cgcatacagc
    18841 gatggtccct cttgctttgt tggcggaatt ggaaactgta gaagaagtaa tgaaagatcc
    18901 tttattctcc gcatatgttg atcagttatt caatctagaa ttgattccta tgctttcttt
    18961 acctaaagat gaattagcca tttatgctga ccaaatcaaa gaacgtttct tgaatccttt
    19021 tgcccatcac aaattagaag cgatttcatt aaatagtgtt tcaaagttca gcacccgtct
    19081 gcttccagtg ttcaagaagt atatcgaaga acaaaatcag gtaccaccgt taatcactgt
    19141 ttcactagct gcgctacttc ttatgtatcg aggcgatcaa gtaaagccac atgatgatga
    19201 aaagacgata agcgaattta ctgatgcttg gtctgataat ggaacagcta ttcctcgatt
    19261 attgcaaaac gctgcgcttt ggggagaaga tttgtctcaa ataccgaatg tgacagatac
    19321 agttcaagaa atagctgcaa agatcgatca aaaaggtgtc cggtcagtat tacaagaaat
    19381 gaaaggaacg atggtccatg cttgaaaccc tgcaaccaac aatgaaatta aatcgacgag
    19441 acagtgtggt cgtcgcatta acccctattc caaaacaaac acaactgacg attgatcagc
    19501 aaacgatcac cactcttgaa gatatcccgc aaggtcataa gattgcattg ggtgatttga
    19561 aagctggaga caacgtgatc aaatatggct atccaatcgg tcatgtgacg acagacgtaa
    19621 ctgccggtca atggctgcac actcataatg taaaaaccaa tctttctggt gaattggatt
    19681 atcgatatga aaaagatgtc catccaagtc attacctttt tgagaaccga acatttcaag
    19741 gttacttgcg aaaaaatgga aaagtaggga ttcgtaatga tttgttttta gtacctaccg
    19801 ttgggtgtgt gaatggcatt gctgaactga tcgtcaaaca gttcaaggaa aaacatccag
    19861 atctgggaca gtttgaccat attacgattt taaagcatcc ttatggctgt tcgcagcttg
    19921 gaaaagatca tcaaaatact cgagaaattt tagcggacgc agtaaatcat cccaatgccg
    19981 gaggagtttt agtatttgga ttaggttgtg agaacaatac agttcctgaa ttcaaaaaaa
    20041 ttttaggtga atatgatgaa gaacgagtga aatttctagt ggctcaagat gtttacgatg
    20101 agatcgaaca aggtgtggct ctattagaag agcttttaac agcggcggaa aatgaccagc
    20161 gcatatcagt cccgctctct aagttgaatg tcggtttgaa atgcggcggt tctgatggtc
    20221 tatcgggaat aactgctaat cctttacttg gtgcattttc tgactacttg attgctcaag
    20281 gaggcagcac gatcttaaca gaagtgccag aaatgtttgg cgcagaacaa gtattgatgg
    20341 cacgagcgga aaatgaagaa gtttttgact caatcgttga cttgatcaat gacttcaaac
    20401 agtatttctt gtcttacggg gaaccagttt acgaaaaccc atctcctggt aataaagcag
    20461 gtgggatcac tacattagaa gataagtctt taggctgcac gcaaaaatca ggaagctctc
    20521 ctgtcgttga tgtgctacaa tatggcgaaa aaatcagaaa accagggctt agtttattgc
    20581 aagctcccgg gaatgatctt gtagctgcct ctgctcttgc ttcatccgat tgccagttgg
    20641 tcttgtttac cacaggaaga gggactccgt ttggctcata tgtcccaacg gtcaaggtat
    20701 ctacgaatac aactcttttt gaacgcaaag gtcattggat ggattttaat gcaggcgtct
    20761 tattagaaca accaatggaa gtaatcttag aatcgtttgt tcaaaaaatc atcgctgtag
    20821 ctagtggaga agaaacgaaa aatgaacaaa atgacgttcg agaaattgcc atttttaaaa
    20881 acggagtaac actttgattt tcataaaaca gtataaaaag aaagaaggaa gatttatgtt
    20941 tttatctgat gattttttat taaaagacga ttgggcgaaa aagctttacc attcctacgc
    21001 aaaaaacatg cccattattg attatcactg tcacttagat ccaaaagaaa tttacgaaaa
    21061 caacaatttc agcaatctga cagaagcttg gttaggaggc gatcattaca aatggcgctt
    21121 gatgcgagct tgcggcgtgc ctgaagaaaa aatcacagga aatgcttctg attttgaaaa
    21181 atttttagct tggtgtcaaa ccgtaccaaa aattatcgga aatccgcttt acagttggtc
    21241 acatcttgaa ttgaagcgtt tcttcggtat tgacttgttg ataaatgaag aaaatgcaga
    21301 gaaaatttgg acacaagcaa atgaaatttt ggcagctcct gattttcgcc gtcgggaaat
    21361 cgcaaaaaaa tcgaatgtag aagtgatttg tacaacagat gatcccgcag acgacttgca
    21421 ctatcatcag ttgcttgctc atgaagaacc agatttgaag gttctacctt cattccgtcc
    21481 agataaagca ctcaatattg aaaaagaagg atttaatgca tgggtcacac tactggaaga
    21541 agcagctgac caaacgatca ccacctatca agaactgata gaagtcttag ggcagcggat
    21601 agaatacttc catcaacatg gatgtcgtgt ttccgaccac gctttagaca tcttacgcta
    21661 taaagaagcg gatgaaacag tattagaaga gatttttcaa aaagcacgag ctaacaaact
    21721 attatcttca gatgaaatcg atgcttatcg tacagaaaca ctgatacatt tgacccactt
    21781 ctatcattct cataattgga cgatgcagct gcacatccat gcttatcgta actgtaatac
    21841 acaaatgttg gaaaaattag ggcccgatac cggctatgat ggaatgaatg atctttcttt
    21901 gactattcct ctgcaaaaat tattgaaccg tgcagaagaa acggatcagt tgccaaagac
    21961 gatcctttat tcattgaatc cgaatgacta tcctgcgatt ttagctttga tgggctgttt
    22021 ccaaaaagaa cagaatggaa aactgcagtt aggttctgga tggtggtaca acgatactcg
    22081 agctggtatg cgcgatcaat tgacacaatt tgctgatggt agtgctttgg ggaattttgt
    22141 cggcatgctg acagattcaa gaagtttcct gtcctacact agacatgaat atttccgtcg
    22201 tgttctttgt gaatttttag gcgaaatggt catgcgagga gaagcacctg aagatgaaca
    22261 acttttagga agtctagttc aagcgattag ttatacaaat gctaaaaatt actttggctt
    22321 ttttgcctaa ttgattaata gtaacagtaa cgaatcaaaa acaggatagt gaaataacgg
    22381 aatccgttta tccacgtttt agagaagaag acggaccaaa tttgctccgt cttcttctct
    22441 tgctgttctg cttcattgcc tataaacctt tatgaaagaa gagataacat gcctactatc
    22501 catattgccg gtgattctac tgcggcagaa aaagatgaaa acaagcgtcc cgagacaggt
    22561 tggggagaaa aaatctctgc ttatttttca cccgagatcc agatcaataa ccaagcaaag
    22621 aatggacgca gcaccaaatc atttatccag gaaggaaggt tagcccgtct agcttcacag
    22681 tttcagccga atgatttctt atttgtacaa tttggacata acgaccagaa aatccaagaa
    22741 gaaagaggta cgcttccctt cggtagctat ttaaaaaatc tgcagatatt ttttgaatgc
    22801 gcaaaaaaag cgaacgtcca aatggtctta ctgacatctg tgacgcgacg agattactta
    22861 gaaaacggaa cattgaatcc agatatactt ggagactatc caaaagcgat gcgtgcattt
    22921 gctgagaaac accatattcc tcttttagat gtgttctcaa gatcacaaga attgttccag
    22981 accttctcaa aagaagagac aaaaaaattc tacctgcatc ttatacctga tacttgtaaa
    23041 aattacccag aaggactaaa agacaatacc cactttagtc cagaaggcgc tgataaagtt
    23101 gcccgaataa ttgttgaatt gataaaagaa tcccgtctgc ctcttgctaa ttatttacaa
    23161 aaaggagttt gagatgtttc cattaaattt aggcatccgt gcccacgatt tagatgttcg
    23221 aaatcgtgaa gaaatcgttc aaaaagtatc acagcttcac ttgtcccata tccaatttgc
    23281 accacaaaaa gcatttcctg aacaacttaa attaactgac atgacacttg ggagtgctgc
    23341 ctattttggt gaatacttcc aaaaaaacaa tatcgaactt tccattttag gatgctatat
    23401 caacctatcc agcaaaaatc aagaagttcg tgcacaagca ttggcaactt tcaaacagca
    23461 aattgctctg tgctctgcct atcaagccaa gatgattgct acggaaaccg gtagtgtctc
    23521 tgtcggttac acaaaagaaa attttactga agaagcttat cagatcgcca gaaattctat
    23581 cattgaatta gtcgctcatg cagaaaattt tggcgtaact gttgctgttg aagcagggat
    23641 caatcatcca atctataact atcagttagc taaacgttta atcgatgaag tagcttcacc
    23701 aaatttaaaa atcattttag attgtgcgaa tttgatgcat aaagaaaatc atgctgatca
    23761 ggaaaaagtg atccgaggtg ccttggacaa tttacatggt tatattacag cgcttcatct
    23821 caaagactat atcatggaaa atggaaagat ccgcatggta cctgttggca agggctgttt
    23881 agatttccgc ccggtactgc attatatcaa atatgagcgt cctctaatgt acgccactct
    23941 tgaagctact cctgagattt atgttcctga agcaatagaa cacttgcagt ttatttacca
    24001 agattgttaa ggagaagatt atggaggaac gaatacagaa aagttatcaa aaatatcatg
    24061 caaatgcttt tcagacattg ctgcatcttt atcagggaaa ttattggcgt tttgccatgt
    24121 caggactctt ttttattatc aaacatcttc catcttggtt aatgccgatc gctattgcca
    24181 atgtaatcaa tgcagcttca tcgaaagatt cttctcagtt gcaggccatt tatttcaacg
    24241 cttttttgat ggcttgtctg attggtcaaa atatattgac gaattatttt catgtaaaat
    24301 accatagcac ttcgatccgt caagtagaag ctggactaag agcgaccctt atcaagaaaa
    24361 tccaagaact atctattcct tatcaaaaag aattgcaatc tggacgaatc cagtcaaaga
    24421 tcatccgtga tgtagaagct atccaaacgt tgtcctctca agtatttgtc agcggattaa
    24481 atatcgctgt caatttattc gtagctttag gtatcacgct ttataaaagt cctgttgttt
    24541 tcatgttttt cttattgact gttcctctcg ccgcattcgt tactctcttt ttccgtaaaa
    24601 ggatcagaca gtccaactca gacttcaggc aagaaatgga agagacttct gcaaaagtga
    24661 tcgaagtgat cgaattagca ccgattgctc gagctcatgg gttagaaaaa cacgaaacaa
    24721 ctcgcttaaa taagcatttt actcaaatct atgaaaaagg attccactta gatatcctgc
    24781 aatctgtttt cggatcagtc agctgggcta gttttcagat atttcagctg atttgtttga
    24841 ttttcactgg gatattagct ttacaagggc gcatccaacc cggcgatgtc gtgatgtatc
    24901 agacgtattt cacgacgatc gtcaattccg tttctggaat gatcacgctg attcctacga
    24961 tttcaaaagg catggaatct atcacttcta ttggcgaact gttgattgct ggcgatgtag
    25021 aaacttatca cggtaagaaa aaaatccaac atttggatgg aaattttcgt tttgaggatg
    25081 tttcttttct ttatccggac tcactcaaac cgattatcga tcatttttct ctagaggtga
    25141 aagctggaga aacaattgct tttgttgggc catctggctc tggaaaatct acactcctta
    25201 acttattgat tggatttatc cagccgactt caggaaaaat ttttctagat gatcagcctt
    25261 tggatgaact ggatatgcga agtgtgcgaa attatttagc cgttgttcct caaaccactt
    25321 tgttgttctc cgcttcgatc aaagagaaca tcacttatgg cttgaaaaac gtatcaaaag
    25381 aacgtttaga tgaagtcatc gaagctgcgc aactttcttc actgattgac cagcttcctg
    25441 atggtctgga taccttagta ggcgaacatg gcaacaagct gtctggtggc caaaagcaac
    25501 gtatctccat tgcacgagcg ttgatacgtc agccaaaaat cattttgcta gatgaagcaa
    25561 cctcagcttt agataaccag tcagagaaaa aaatccagca agccctgaat tacctgacag
    25621 aaacacccac tacttttatt gtggctcatc ggctttcaac gatcaaagag gcagataaaa
    25681 tcgttgtgat cgatgaagga aaaatcgttg aaataggtac ttacgaaaaa ctgttagctc
    25741 aaaaaggcta tttctaccgc ttgaaaaatg aatgacaaaa aataggagct atgacaagac
    25801 ttttgtcgta gctcctattt ctgaataaac ggtgttctcc tgccaactcc tcggtcacaa
    25861 gtcacaatag tcatcaaaca tgagaagctg ttttttgaaa ttgttccttg taactcagag
    25921 ttaaacggta ggctcacgac ctcttggtaa atggtgttca agagacagcc tatcggcaat
    25981 aagaagcaat tttgtcatcg ctcaaggccg aacgtctctt tcacaccctc tgttgtttca
    26041 taaaatagtt ttattcgctg taaaggtcgc gaactggtgt catgatgata cggttcaagt
    26101 cattgatgat tgtactgaag ccttgctctt tttgcatcaa gtcattgatt acttcttctg
    26161 attggatttt cacagccatt tcttgtgctt tcattgcatc ttcgtcgctg aactcttgtc
    26221 cactcatttg tttttcttgt aattcgattt gcatcgcttg gaagtctttg aacagtttgt
    26281 gtgcttcttc gtttgcttgt actttctcgt acgcttcttt caacgctttg aattctggta
    26341 attcacggat ctcacgttcg atttgattcg cgctatcgta gatgtttgac atgttatgcc
    26401 ctcttttctt ttagtagtta ctctctattg taacatgggg ttattcgttt tcaaaggggc
    26461 tactctcctt gatatttccg ccagaggtct ttagccgctt caccaattcc ctgaacgcct
    26521 tctttcacca tatcgccgaa gtttttcagc ccttcttttg cttgttcacc atattgattc
    26581 aagttatctt gccaatttcc agactctcca ttagaatttc ctgtgtttcc aacttgatct
    26641 gcagctacca cttgtcctcc tgtggcatag gcatctgcta cactaaatcc tgtttttgaa
    26701 gtatacggaa ggatgccttc tgccgctgat ttgaagactt tcccgacaac atttccactt
    26761 gtcccttcca aatagtgcag cttgctggat tcttcgtaac ccatccatgt cgaaatcact
    26821 acgtctggcg tgtaaccgat catccattgg tcatttgctt ttgtggcatc gaaattagtt
    26881 tctgtggttc ctgttttccc tgctatcgta taacctgcag gattcgcagc agccgctgtt
    26941 ccatttgaaa atgtaccaag aagcatgctg gtcatttcat cggctgtttc ctttgatatg
    27001 acctgttttg gttttttgtt tgtgttatcg acgatcaccg ctccagtcga atcaacgatt
    27061 ttcgtgataa gatgcggcgt atacatctga ccatcattgg caaatgcact gtaagcaccc
    27121 gccatgatca atggagatac tcctttttcc agtccaccaa gggctaagcc ataatactta
    27181 tcagattttg ctaaaggcag gccgaattct tctgctttat catatccttt ttgtaaaccg
    27241 atctcatgca aaagccatac agtaggcaaa ttcaaacttt gtgcaacagc ttgatacatc
    27301 ggtacttctc cttggtatgt cccgtcaaaa ttatgcgcat cgtaataact ttgcggttcg
    27361 tctttcaaga tactggaagg cttgtatcca gcttccagtg ctggtgtata tacgcttaat
    27421 ggtttgatcg ttgatcctgg tgaacgacgc atctgtgtag caaagctgaa tcctctaaag
    27481 acatgatctc ctcttcgtcc tactagtgct tgtacaccac ctgtattcgg atctaaagct
    27541 actgaagcag attggaccat ggcaccatct gaagcatttg ctgggaataa tgcactgttt
    27601 tgataagtag cttctaattg cttttgataa tcctgatcta aagctgtata gattttatag
    27661 cctttattca tgacatcttc ttcatcaaga ccatactcgt taactgcttc atcgatcact
    27721 gcatcaaaat aataaggata acgataacca gaatctgaat tatcgtatgt gtcattaaga
    27781 tacgcgctga tatctgtctg gctttctgct tgttcttgtt cttttgtgat ttttccgttt
    27841 tcttccatta cacttagtac tgtgtttttc cgatcattcg cattttttgg gtgatcgatc
    27901 ggattataaa taccaggacc tttcagcatt cctgccaaag tagcagcctc agacagcgta
    27961 acattcgttg catctactcc aaaatacttt ctggaagcgt cttggacacc ccataccccg
    28021 ttgccaaaat aggcattgtt aagatacatg gtcaagattt cttctttgct gtacttcttt
    28081 tcgatctcga ttgccaaaaa taattccttc gcttttcgat caaacgtttg gtctaatgtc
    28141 agatacgcat ttttggcaag ctgctgagtg atcgtacttc ctccgccccc tgatgttccg
    28201 ccagacaaga ccattcggac tgctgcacgg gcaatccctt tgatatcaaa cccgtgatgc
    28261 tcgtaaaaat tcctatcttc tgtcgagatt actgcatctt ggatataggg agaaatcgcg
    28321 tttaattcta catatgttcc tttttgggcg tacagtgtgc cagcttcttc gtttttctta
    28381 tcataaatga ctgtcgattg gcttaatcct gattttaacg tttcgacatt tgcttgtttt
    28441 gctaaaataa agaggtagat acttgtcact aagacacaga tcaaaccaat cagcaaaatc
    28501 aatttgttca catgatattt tttccagatg cgctttcgcc atcgatggaa ttgtccgaga
    28561 tatggtctga tccaacgcca aaaactgatc aaagcagctt ttatcttttc taaaatgctt
    28621 tgaaaatcca tttgcttctt ctcctgtcta tttgattcaa tcgtcttatt ttaccatagt
    28681 gaaagagtgt tttcttcctg cttaagttgt agttcaatat gtttttttac gttagtgcaa
    28741 gtagagtaac ataagaatga acagaaaaat gtaagttttt tcttaaatat gttttaaaaa
    28801 tggtacaatt agtctgcgat tgcactgaaa ggagtacact tgtgaaaatc acgatcactt
    28861 tacctatgaa ccatcctgaa acaacaattc gcgacttgct tgaaaaggaa tggttagttc
    28921 ctagaaaggt tcgacacttt ttgcgtatac gtaaaaacgt ttggatcaat caacaacctg
    28981 ctttgttcca tcatgaagta aagggcaatg accagatcac attgttattc gaagaaacag
    29041 attataatta tcaagaagtt caattaggag atgcgaagca catccgtgtt ttatatgaag
    29101 atgagcattt gattatagtg gataagcctg caggaatgaa gactcatcca aatgaaccga
    29161 cagaaaacag taccttactc aatcatttag ctgcttatct cgacactaaa cagcaacgac
    29221 cttacgttgt ccaccgcttg gacaaagaaa caagcggtgc cgttttattt gcaaaaaatc
    29281 catttgtctt gccgatactt ggaagaatgt tggaacaaaa aatgatctat cggagatatc
    29341 aagccatcgt atggggagaa attgccaaag atatgaccat caccaataaa attggacgag
    29401 atcgccatga tcggagaaaa agggtcgtag atgataaaaa tggtcagagc gccttgacac
    29461 atgtcactgt caacaaagta ctttctgaac aaacacaagt ctattgtgtt ttggaaaccg
    29521 gacgtacaca ccagatcaga gttcatttag ctcataaggg acatccgatc gttggagacc
    29581 ctctctacca aaatagaaca gctgaacgac tgatgctcca tgcattagaa ctgcacatga
    29641 tccatccatt tactcaagaa aaaatagtcg gtaaagcttt acctggatta tgggaataga
    29701 aataaaaaag aacagagtct tatcagcaga taatgataag actctgttct ttttgtgtaa
    29761 tttgtctgct tacttttgat gttcgtgttc tgctaacagc aaactaccga taatcccact
    29821 ttcattcggc agtccccaac gaacgatata ttcacttact tgaggtgttt ccatataacc
    29881 acccatcaat tcaacaaatt gctgacggat tttttgaagg agatgatcct gattcattac
    29941 cccaccgcct aaaataatct tttcaggtgc taacgtcaac gtatagttga tcagtgcttg
    30001 agcaatataa taagcctgaa tagcccaaac aggatgatct tgcggcagat cttgcccttt
    30061 gatccctgtg cgtgcctcaa tagacggtcc tgctgctaat ccttctaaac aatctttatg
    30121 ataaggacaa gtaccttcat aatggtcatc tggatggcgt ttcacccaaa tatgtcccat
    30181 ttcaggatgt gcgatccctg aaaaaatttc ttcattgatc acgacacctg caccgattcc
    30241 tgttcccacg gtaaggtaaa tacaactatt ttttccttgt gctgctcctt gcttcaattc
    30301 cccatatgca gcagcattga catccgttgt ccaagcagca ggaatattgt agcgttcctt
    30361 gattgtgcca agaaaatcga attgtgccca cccttttttg ggagttgcca aaacgtatcc
    30421 gtaattatct tttgttttgt cgataccaat cgggccgaat gagccaatcc cgattccctt
    30481 caattcataa cgatcaaaaa attcaaatac ctgatccaat gtttcttctg gtgttgtggt
    30541 agggatactt aatttttcta aaatttctcc accatctgaa accgcacaca caaattttgt
    30601 tccgcctgct tctattccgc catacattcg ttttcctcct cgaatccgat tgtccgaatc
    30661 tcataaggtt ggattattgg gacaaccgct tgcttcgttg gttcttcaag cagattcaat
    30721 atgacagttt ttccattgtc tttagttatt tccaattttt ctaaatgact agacatattg
    30781 aaacctctta ctactacttt atcactaaac ttgctgcgtt tcaaagctgt aattgcaaac
    30841 gtttcacttt ttatcgttaa atattgttgt tttgaaatca atgttccttc atgatgtttg
    30901 atttgttgag tggaaaaagg aatctgtgct gcgtatgcat gcaaataagt agagaatttt
    30961 tcttctggtc catgtagttc aatgctgtaa ttgaaccgat gctcacctag acactgtgct
    31021 tctggtgttg gaaaatatcc ccaatcgcct agttctccca cactgcgaag tagcgtcact
    31081 gcgatttgtc cgtcttctgt tacttcatat tcattcaaac caaagtttcc gactgttaca
    31141 ccgtactctt catcatgaat attgacaaat gcttgctgat gttgcggatt cgtggggttt
    31201 tcccaacttt tagacacatg gtttgggcgc ttcaccactt caaaaatact atctgcttcg
    31261 tgatgttcca catgtaactt tgtaggaaac aagacacgta agcgatgatc tttcatctga
    31321 ttgtcgagag tcgtttcaaa gtcgattttt ttactatctt ttctgattgt catgattgtt
    31381 tttatttgaa ggattcgttt ttctttggat cgttctgctt ttcgataacg aaattccaca
    31441 accatctgct gttctttttc taaacgctcg tctgcagaaa cagggatttc catttcctgt
    31501 agaatactta tttttgctat ttccggtgta tctactaata cttttacttc agagttttct
    31561 ttgttactgg ataggattgg ctgatcacag aaaggttgtt taaagatata ctcatttcct
    31621 atatcgccga tatcttcaaa aatatgcaga ttattcaact ttcgtttgtt tttgcgatct
    31681 tccattgtaa tggtaccatt tttttcaatc gtcaaatgta gagggccgtt ttcgatgatt
    31741 cgtccactct gatgaatcat tgtttccttt tcttctgctt tcgcttcccc ttctacaagc
    31801 gcgaatgttt cccaagaaaa tgctggcatg ttttctaatg gtaaagtcac tttgatcatt
    31861 cttgccatat atggcacacg gaaacggtct ttcggcaaat cataaccaaa ttggacagtt
    31921 tcttcagata aaatagctgg tatagtggcc ccactttttg tttctacatg atacttacgt
    31981 ttaggcaaat tttctaactc ttgatatagt tgttcaggta ttccttcagc gaatcttttt
    32041 ctttctaatt cgatggttat ttctgcttca ccagttttat ccattcctgc agtatttaca
    32101 atgacaaatg gaaagctgtc ttttgggaat ccggtcgtat cgattgctgc ggtcagctgt
    32161 tctaaggaat cttgtgcaag atactttcca acttcatctg ctttttcaaa acgaggtatc
    32221 atttcccgat ggacagaatc gactgaacaa ccgcaaatac tgtcgtgagg atgattttgc
    32281 attaatgtct tccaagcata atctaattga tcatggggat agtttccact aacttcataa
    32341 gccatcgtcg caagtggttc tgtaatattt tctagctgtc gctggacctt ggtattccat
    32401 tgttttaagt aaacccgagc agatgctgta ttggctaaag tataccagcc atccgtttct
    32461 tgacttgtca attctccttc tactgagcct aagtcttctg gcacatcttt ttgaactgct
    32521 tccagatagt cagtaaaatt agaatgaata aactcgtaat ctggaaacag ttcatttgcg
    32581 agatggattg cttttgatat atctttttgt accggttgat gatcaacacc attcatcatc
    32641 aataagtggt tcgtagaggc atattgttct gcatccgcta atttttgctt ccaaaaggca
    32701 agagctgctt ctttttctac tggaatctca ttcccgttgc tataccaatt ggcaaatagc
    32761 agaccaaaga ttgctgtttg atcaggtcct ttccaccaca tctctgagta ttgggaagaa
    32821 tagttttctg cttccaaaac ttgattatca aagccaattg gttttacacc acggccaaac
    32881 gctgctgctg aaataccagc ttgtttcatt aattgaggcg tctgtcccat atttccaaat
    32941 gtatcgggaa aatagcctaa catgacaggg gttccccatt tacggctttc ttccatgcca
    33001 atcagcatat ttctcacgtt cgattccgag ctgataagaa aatcatcttg caagatataa
    33061 aatggtccaa ttctcaattt tccttcattg attgcttgtt ggattgcctg tcttttttct
    33121 ggccttactt gaagatagtc atctaaaata atcgtttgcc catccaaatg aaaactgttg
    33181 aaggacggat caacttcaaa tagctctagt agatcgtcta tcaattcaat cagtcgcatg
    33241 tgatgctgtt cgtaagccat gtaccattca cgatcccagt ggctatgaga aatgatatat
    33301 actttctttt tcatttacga tacctcctac ttttctactc taatatcaaa gtaatccatg
    33361 actaattcgc aaaacatcat atttgcccaa gaaaaccatt ctctcgtata attttcagga
    33421 ttgttcacat cgaatccttc gtgcataagg tgtgttcctg cgtcagttgc tacaagtaaa
    33481 ttcaatatcc gctctttttc agatttgtct gacgttgtca ttccttccat agctaatgca
    33541 atcggccaaa cgtatttttc tggcgtatgg gaggatccaa ttccttttgc gtactttcct
    33601 tcatagaaat atggattttc tttactcaac aatgttcttc ttgtttgcag ataatcttcc
    33661 tcttgttcag aacaatatcc caagtaaggt gcggcgatca gattgggcac attactgtca
    33721 tccatgactg tggcgtttcc cattccatct acttcatatg cataaatcgt ttgccctttt
    33781 tgattaaccg ttcgtccgtg attctgaatc ccctcttgga tctctttctt caatttctgt
    33841 gtttgctgta caatttcttt atcatcgagt atcgtttgaa aaatttcttc cacataacct
    33901 aacacgacca ccgcaaacat attggatggt accagatatc cataacggca agtatcatcg
    33961 ctaggacgga aaccagacca tgtcattcct gtaaaagctg tttttgcacc tcgcccgtca
    34021 ttgatcagcg tatcttcttt tcttgtcgtg tctcgtacaa atgtataagg agagtgttca
    34081 tgatcttgct ctgttttgaa cacgtccaag atttttttga taccttcaat aaaagattga
    34141 ttgaattgat cagtttttcc tgtatttttg taaagaagat aggccaactg tattgggtag
    34201 cataaagaat caatttcata ttttctttcc cagatccaat cgttcatttt tgtatgatct
    34261 gtttggtggc cagcaccgtt agcttcttcg ttgaaggcat tcgcatatgg atcgatattg
    34321 atataaaaaa attgcttttt gactaacccg ctgatcatgt cagctaattc ctcatcttct
    34381 tttgcaatca ctaaataagg acgaacttgt gcagtagagt ctcttaacca catcgctgga
    34441 atgtctcccg tcaataaaaa ggtggttccg tcattttgct ttttgactgt tgtcaacaga
    34501 gtattggcaa atgctgctgt aaaattcttt gcccagtccg catggatatc cccacatttt
    34561 tgtgtgatat cttccataaa tttctgcacc gattttggta tatctttata agccactaat
    34621 aaaaccttct ttctatttga tatatcttta aagattatac ttcatacagc caattatgtc
    34681 tacgctttca aaaaatatct ttttatttac tttttcatcc attatggtat tatttgaata
    34741 tgatatatca ttaagaggtg tttatatgaa tcaaccattg taccagacaa tatttaatga
    34801 tttgcaaacg gctattttaa ctggtgaatt acctgtagac agccaactac caactgaaaa
    34861 agaactttca cgtacctata aagtaagcag aatcacttcc aaacgtgcat tgactgaatt
    34921 agagcaatta ggacttattt atcgcgttcg cgggaaaggc agtttcgtta aatccgcttc
    34981 tccggaacag attcttgctt ctcctacaaa aacacgacgg attctgtttc ttcttccata
    35041 tgtcgctgat ttgtctgttg gtgattttag caagggattg aaccctgtga tgcaagataa
    35101 acagattgat gtcatgatga cgacgcttga ctttctacaa aataaaacag caaaagagct
    35161 aatcaatgaa tttgacggat tgatttatta tgctcttgat acagaacaac atcttgactt
    35221 actttttgag ctttcattga aagaattccc tgtcgtcata ttagataaaa aaatctatga
    35281 gttacctttt cctaccgttc tttccgataa tttccaaggc ggtgcgcttg ctgcctctta
    35341 tttgatcgaa cagggacatt caaaaatcgc ttatttgttt ggtacgcaga ctcatccaca
    35401 atccgttcga cagcgttacc ttggttacct tgaagcattg gacaaagctg gtttgacttt
    35461 ccacacttca ctggatgaca aaagcgcgac attagatgaa ttgctttctt atatcactaa
    35521 gtatgaagta actgcttttg tttgtgaaaa cgatgttgtc gctatccaga ccatgaatct
    35581 cttgagacag catggttatc atatccctga agatttttca attatcggat ttgatgatat
    35641 ccaagcagca gctcttgttg acccgccact gacaaccatc gcacaagatt ttgaacagtt
    35701 aggtaagctt gcgggagatg ctttggtcac ttggttagag acaaaagatc gaccatccga
    35761 tagtaaattt ccagtttcat tcatcaaaag acaatcaaca aaggagaaat aaaatgaata
    35821 tcggtcaaat agatactaga catgcaacag cgaatcagcc aaatttctct aatgggaact
    35881 gccagcctta tactggcgtt ccttttggga tgaattattt tgcaccgcaa actacggatc
    35941 aaaacggcag ctggtggttt catcctgatg accgcgtttt tcaaggctac cgtcttaccc
    36001 atcaaccaag tccttggatg ggtgatttca gtcatatgct gtttacgccg atcaatggaa
    36061 aactacagga gaacaccctt ttccatgctc aaagttctta ccgcccagaa gaaagtatat
    36121 tttgcccgac tcatctgtcg atctgtcaat tacgttatgg catctgttct acattgatac
    36181 ctagcatgta tggcggaatc ttatctattg attacagcaa aaatgacagc ggtttacttt
    36241 tatcctttcc gggacgccat cagctctttg ttatagatcc ttacacagtg gaaggaaaaa
    36301 tcacacattt tgctggttca gaagatcctg atttttcttt ttattttata ttgagatttg
    36361 aatatccttt aacaattgat gagttagctg tttccaacaa aaatagcgac tcgatcccgt
    36421 tgtattttga acagacaaaa aaacaaacta tccgatttgg gacatctttt atcagctcgg
    36481 aacaagcagc ttataacctg gaactagaac aaaaccaaag caaagagact taccttgctc
    36541 acagtcaaga acaatggcag tcctattttg atcgtataca gctcacacac catgatcaac
    36601 aacaattgcg gacgttttat cataatttat atcgggtgtt tttatttcca caaacatttt
    36661 atgaaattga tcctagtggg caaaaaatcc actatgatac gactagtcga tctataaaac
    36721 ctggtgttct ctatacaaat aatgggtttt gggatacgta taaaacggtt tatcctcttt
    36781 attctttgat tgctgtagaa aaatacgaag aaatgctgga aggttttttg aacagctata
    36841 gagaaagtgg ctatttgcca aaatggctgt ctcctgatga acgtggactt atgcctggga
    36901 cattgatcga tgcggtcatt gcagatgctg ctgtaaaaga tatccgacga gacttgatgc
    36961 ctgaatttct aacggcaatg gaaaaaggtg ctacggttca aagcaaacat ccaaattatg
    37021 gacgccaagg aacagaagat tacttgacat atggctatgt tcctaatcac taccacgaat
    37081 cagtcaatca cacattagac tatgcttata gcgatttttg tattagccaa gttgccaagg
    37141 tactagaaga agaggaaatg acgaagaaat atcagcagca atcattgaac tatcaaaata
    37201 tctttgacta tgagtcagga tttatgcgtt caaaagataa agatggcaag ttcagagaac
    37261 cattttcagc taatcgttgg ggacaagatt atgcagaagg aagtgcttgg caaagcagct
    37321 ttgctgttta tcacgatttt tcaggactga tcggaaaata tggtggcagt gagagatata
    37381 aagaaaaact cattgagctg tgcaatcagc ccccaacgtt tgacccagga ggatatggtt
    37441 ttgagatcca cgaaatgagt gagatggccg cgatagaatt tggacaatta gctatttcaa
    37501 accagccaag ttttcactat ccttacttgt tcagttatat aggacaacct gaaatggcac
    37561 agccactgct taaacagttg atgactcaga ccttcgacga ttctccaatg ggttaccctg
    37621 gagatgagga caatggaagt atggccgctt ggtatatctt caatagtctg ggattttatc
    37681 ctgtcacgcc tggaaccggt gaatatgtta tcggtatgcc attaattgac aaggctgttt
    37741 tgcttttgtc aaatggaaag gaattgacga ttgaagcaac gcctaatcgg ccgcagcaac
    37801 aatttatcca taaaatcaaa cggatggaca aagaatatac gaaattatat tttacccatc
    37861 aagatttgct gaaaggcggc acgatctctt accaattagg gatcatccct caccctaaga
    37921 aatatcaagc aaaagatttg cctttttcct tatcaaacta taattaacaa gaggttgtga
    37981 aaaagctgtc ctgcatcaag taataagaac agccaagcaa aaatagatcc tcatattttt
    38041 cgtttggttg gtcttattac cgcagatgca agcttttgaa caccgtttat tgagaggttg
    38101 tgaaatcagc gttctgacct gagtattaga gagacaaatg gcaaaatagt tcctcatatt
    38161 ttgccatttt ttgagctaat accgagggtc agctgattga acaccgttta gtggagaggt
    38221 cgtgaaaaag ctgtttagtt tcgagtatta agaaaaattt tgtgaaaatg gctttccaca
    38281 tttttgcata aatttggctt aataccgaga aactggcttt tgaactccgt ttatcgagag
    38341 gtcatgaaaa agctgccttg cctcaagtaa taagaacagc ccaaaattag atttctagac
    38401 aattttttat aagttatttc attacattgg ctttttcttt tatgattctc cactgttttc
    38461 tcggtactct ttaggggatc tgccgacgat tttcttgaat aatttagaga aatacgtggt
    38521 gttgttgtag ccaatctcac ttgctatctc attgatgttt tgattactgt gcaaaagcag
    38581 ttgtttcgct ttttccatac gtaaatggtt caaataagct gaaaaacttt ttttcgtttc
    38641 ttttttgaac agctgtccta gatacatgac atttaaatgc aggttttctg acacttcttt
    38701 gagtgtaagt ggttcttgat aatgctggtg caggattacg attacttttt ccacattgtc
    38761 actgtacttt ttctgtctct gatgcttttt gatgaatgac agaagtagct gctgaagttc
    38821 ttgcaccgtt tttgcctgat ggattttttc gattccttgt aaatattcct catcttccag
    38881 atcaatcaat tcccgttgaa tctccataaa cagtaaaaaa ctggaatatc gtatatcttc
    38941 cggcatcatg gctgctaact gaaactgttc aaaaaaatca gtgatcattt tttgagcgac
    39001 ttccaattgt ttattttgca gcacgcgtct gaaacttgaa aaatcgatgg tttgatcttt
    39061 tgtttctgct tgtactgcgt aaaaaacatg ctcgtcttta tctccataaa attgatgcaa
    39121 ctgtagattg tctttcgctt gttggaatgt ttcagggatt ttgtccaaag aatctgtctc
    39181 ttccccgata ctgatcagcc attcctgatc aggaaagttt tcttgaacaa aacaacagaa
    39241 gccatcaact tcatctgcct ccaacaacaa cgtgatcaat acacggtctc cgtaatttcg
    39301 ttgatagtaa aagatttgtt gccgttcttg caaccatcta ttgataagag tatcatgagt
    39361 tcttgccagt tgaatcaata ctatcctatg ctgcttctca cttatttgtc ctattatttc
    39421 ttcttcactt atttcatcca attcttcatt caaccattga ctgaataaca gttcttgata
    39481 gatttcttgt tgattttttt gttcattttg ctgatctaac cgtgttttga ttttcttgag
    39541 gctttcaacc aattcgaact tattcacggg tttcatcaaa taattcacgg cgcctaattg
    39601 catacctcct ttcaaataat cgaattcttg gtatcctgat aaaatcataa attcaaaatt
    39661 atgacgttct ttttgagccg cttcaataaa ttccagtcca tttatttctg gcattgtcac
    39721 atccgttact actaaatcaa tctgtcgctg ttcaagtaca gctagtcctt ctttggcatt
    39781 ctctgctgtt gctactattt gaaagcctaa actttgccaa tcaattatct tttttaaccc
    39841 attcaaaatc atgtattcat catctaccaa taacacttga tacacgctca tccctccttg
    39901 aatgtaatag aaacggtcag tccacctgtc tcattattct caagcgacat ataataactg
    39961 ctgccgaagc tggcacgtaa acgttcattc acgttttgca gaccgataga accgtgaagt
    40021 tccactttag gatgagacag tttttcttca atctgtttta gccgtttttc agtcatccct
    40081 tttccattat ctttgatgat gatccttacc cgtttgcctt cctgcaagac tttgacactt
    40141 agcgcattat cgaatcgagt aaaatcaata ccgtgtttaa aataattttc aactaatggc
    40201 tggatcacaa atttaggtac ttcgatcttt tccaaatcag gatcgatcat aaaatgatag
    40261 gctactcgat taggataacg catttgatac aaatagacat atttttcgca aaagtccagt
    40321 tcttctttta aagtgatcgt tttctcttga ttcgtattgt ttcgaagcaa agcggaaaac
    40381 gcatacacaa catctgctaa ttcttcactt ccttcgctga ttgcatacat tcggatatat
    40441 tctaatgtat tatataaaaa gtgtggattg atctgtgctt gaagcgcccg catatgtgcg
    40501 tcttgctgtt tgatttctaa tttgtagatg tcttctacaa actgattgat gctatccaac
    40561 atttcgttga taccgattga aagttccttc agttcatatt ccgtgtttgt tatgtcgatc
    40621 cgcgtctcta aattccctgc tgctgtttga tccatggcct gcatgacgat agatacttgc
    40681 tgtgaatatc gtttgaatgt tttataaaga aagataagca gtatattgtc taaaataatg
    40741 ataccaatga tcagcggggc aagatttttt cccgtttcga tttttacact ttttttatct
    40801 agtacagcta atatacgata atcatcagac agttcttggt gttgaacaaa attgtttttt
    40861 tccagatcgt caatcggcaa ttgtgaattt ttcttcagac tctctgtgat gatcatttct
    40921 tgtctttcaa acgtttcttt agctatttta ttatcatgga atgtgtatag tcgatttgtc
    40981 gttccagaaa tcatgtataa agagagtcca ttgaatgacg ttagattttc taaaatcata
    41041 tctagttctt tcttttgaaa accaataaaa atacttcctg actcagctcc tccttgtgtg
    41101 ataggcaact tgatataaaa ggcgtctagt aattttggtg tccccttttt tatttcccca
    41161 cctttgtttt ctctagaaga ttcataatac tccggtagtt gattcaacac gatatatatc
    41221 gcacttagtt cttcataatt tgcgtagaga tctttgattt gatttgggaa agaaactaat
    41281 tcatcggtac tgttttgctg ttcgtataca tacatcagat actgatctat gggttttgtt
    41341 aagtaggtcg tcacattttc gatcttttcg gaattcccta caagctggtt tttgatgatc
    41401 ctactcaagg ttgttttatc attcagcatt ctgcttgtgc tttgaacggc atcacttgtc
    41461 atgttttctg cctgtttttg attttgttcc caagtatgga tactaaaaat aacggtagca
    41521 gtcatcgtta caccgatcat gataaccgcg tatttccgca acagacgatt tagtaaggtt
    41581 ccttttgaaa atagaatccg aaacttattc ataactttcc aactgtttat ctaattcttc
    41641 acgccaatgt gttgtcatcg taccattcca aaccgtcaaa agaccaatga caccaaacag
    41701 gatcaatcct ttgaattgcc aagtcacgcc taaaattata ccgctaccaa taattatttt
    41761 tagaaaagac gaaaagctgg aaaaaaccga aataaaactt agctttagca gattaggaac
    41821 agagatcgta tattcactat ctaaaatcat actgtattga taagtagcaa ataggcagag
    41881 caaggcaaaa agtaaaataa aatcaatagc taaaaaaatg agccctttga tttgaacaga
    41941 taaatataaa ttgtaactca ataaagcagt accagataaa aataaactaa atagcagatt
    42001 gcctcttaaa aaatttcttt tatatatatt ccagcctctt tttattgtga tttctttata
    42061 ctcgaatccg tacaaataaa atagttcatt gactgttttc catgccgcac ctaatccaaa
    42121 gatgatcccg ccggcaaatg tatatatcca gaaatacaag gttagtttta taatgaccca
    42181 gatccggata aataactctt ccaaaacctt ccctaacata tcttctcctc caaacattaa
    42241 gtttatactt tctcattata caaaaaaagg ttgggcaatc agcaccttga cctgattatt
    42301 agagaaacaa acggaaaaat agttcctcat attttgtcgt tttgtgagcg aataccgagg
    42361 gttagctgat tgaacaccgt ttaccaagag tttgtgaaat agccgcttag tttcgagtag
    42421 taagaaaaat tttgtgaata tggctttcca catttttaca tatatttagc ttaataccga
    42481 gaaactggct tttgaacacc gtttattcgg aacaagaggt tgtgacacgt cttttgtcac
    42541 aacccctttg tctttattca ttattattct tgtcggaact catcatattg tttttgcatt
    42601 tctttcaata ccttatcata gccagccttg tccaaagcgt ctttcaattt cggtaatgtt
    42661 tcatctggat caacagtccc tgtattcaat ccatctaaat attgattcat gacattagaa
    42721 atattactta attctgtttt gacactgtca gtgttgaagc tgaatcctaa aatcggtgaa
    42781 gtttctgcat cagcaatcga ttgatcacgt ttggcaatca tgtcatccgt aatagactct
    42841 tgtgtatata gaatcttgtt gtttcctgta ttccaagcag acatatgtgt atttggctga
    42901 taaccatcta gcaatttgat tttatcatct gaaccatcga ctttttccca tgcttctccc
    42961 tcgatccccc aaacaagccc attcaataat tctggatcac tgttaagcag ccctagaagt
    43021 tctactgatt tttctttatt ttttgaagta tttgatacga caaaatttgc catttgtgct
    43081 tgagctgtag attttagtgg aacagtaatt gcttttgaga caagttcttg ttttgctgca
    43141 tttgtcaaaa ttgtatcgcc ataatcatac ggtccttgtg tttctccacg cattagccaa
    43201 gtatttcctt ctagtggata atccttattg ctcgttgcag cgtcacttgg aatgagtcct
    43261 tgtttgtacc agctatgcat tgtacgtaac agtccgatat acgtttcatt atcgtattgg
    43321 ttgatgattt tcgaatcatc tccatccaga tcaatagcaa atggtaacgt attccctaaa
    43381 ggataatcaa agttcccttg gactttgaag ccttgtccaa tggcaaaagc tgccgtgttt
    43441 ggttcttttt catggaattc cttcaagact ttttctgcat cagcgtaaga ttggattcca
    43501 tcaattgaaa gatcgtattt atctagtaaa gctttgttaa aagtaagtac ttgttgagca
    43561 aagacatttc cattgactgg gaaagcatat aattttccat tgaccagatt tcctttaata
    43621 tatgcttcat ctaaatcatt ataagctttt tcagcgtact taggcgctaa ttcagtcaaa
    43681 tcagcaaagg ctcctttttg ggcattggaa acatagttgt tcgcaaaggc gatatcataa
    43741 ttttccccgg aagaaatgat gacgttcatt ttcttttcgt aatcgcccca gccaatgtaa
    43801 ttgatattga cttttacacc gatctctttt tcgattcgtt tgtttgctac ttccatcaat
    43861 tgatcgaaat tatctggttt atctccgatt tgatacatct gcaaagtgtc cgaattgcta
    43921 gcttctgcct ggtctttaga accagaacct ccgcatgctg ctaatgttcc caacaacaac
    43981 gctgaaacac ctgtaaaggc tagtttcttc cataacttca tttttcttcc tcctaatttt
    44041 atgatttata tttttgtttc tttattcttt tactccgccg attgttagac ctttgacaaa
    44101 atgcttctgg aaaaatggat agctgatcgc aataggcaac gtcgagatga ctacgatcgc
    44161 cattctggca gattctcctg gaactgaggc taatccgcct gtcaattggc ttcctgctcc
    44221 tgaattttgt gtcagatatt gaatattact ttggattttc atcaaaagat actgtagtgg
    44281 aactagctta tcatcttgga tgtatagtaa agcattgaac cagtcattcc agtaacccaa
    44341 tgcggcaaat aaactgattg tcgcaatacc tggaacagct aacggtaaaa cgatctgaac
    44401 aaatattcgc agttcgctag ctccatcgat ccgtgccgat tcaataatcg aatcagagac
    44461 cgaacgttta aagaatgtgc gcatcacaat aatattgaag ggactgacag ccattggcaa
    44521 gatcaatgcc caaatcgtat cttttaactg aagaagattt gtcataacaa gataattggc
    44581 caccatccct ggtgagaaca acatcgtaat caaacaaaaa acggtaaaaa aacgacggaa
    44641 tggaaagttt gaccgtgaaa tcgcataagc atataaagaa gtagccgtgc tattgatcag
    44701 tgtacctaaa acagtaacaa gtacagtgac gaaaagcgct tggaaaattt tatcctgcat
    44761 ctggtcgaac aagtacgtat aaccaaatat actgaacttt tctggccaaa aactgtaccc
    44821 gtgagtcgac aatatggact catcggtaaa agagatggcg ataacaaata taaatggcaa
    44881 aatacaggaa atagcaaaga gtgcaatcat tatattagag aaaaaattag ccgtcttatt
    44941 gaaagagcgg acttggactc tctccacttt cttttttttc atttttttcc tccttagaat
    45001 aatgcagaat cgctatcaaa acgtcgggcg atcgtatttg tgatcatcaa caagacaaag
    45061 ccgacaactg attggtaaag tcctgcagca gccgtcatac caaaatctcc tgtggatgtc
    45121 aggccattgt agatatacgt atccaaaact tgagtcacat catataatgc accggaattc
    45181 ctaggaatgt tataaaacag gccaaaatca gctcggaaaa tgtttcctac ggctaaaatc
    45241 gttaaaatcg tcattagtgg aataagttgt ggtatcgtca catgtttgat ctgctgccat
    45301 ttgctagctc catcaaccat agcagcttca taataagtcg gatcgattcc cataacagat
    45361 gcaaagtaaa taatgctgtt ataaccaatc cctttccatg taccaatgaa caccaagata
    45421 aacggccaat atttcggttc attataccaa ttgacagctt ctcctccatg tccagtgatc
    45481 cactgattga atattccttt gtcaggactt aaaaaagcgt aaacaaagta actgataatg
    45541 acccaagata gaaaataagg tagtaaggac atcgtttgat aaactttaac gagtcgttta
    45601 tttctcaact cgctcataat gattgcaaat acgactgaga tcagcagatt ca
//
LOCUS       DAIGOK010000006        43093 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.006, whole genome shotgun sequence.
ACCESSION   DAIGOK010000006 DAIGOK010000000
VERSION     DAIGOK010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43093
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.006"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            536..1001
                     /locus_tag="NOA01_000237"
                     /pseudo
     CDS             536..1001
                     /locus_tag="NOA01_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323211.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            1344..1600
                     /locus_tag="NOA01_000238"
                     /pseudo
     CDS             1344..1600
                     /locus_tag="NOA01_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            1987..2214
                     /locus_tag="NOA01_000239"
     CDS             1987..2214
                     /locus_tag="NOA01_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCI0631472.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            2230..3396
                     /locus_tag="NOA01_000240"
     CDS             2230..3396
                     /locus_tag="NOA01_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCI0631473.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            3631..4674
                     /gene="hrcA"
                     /locus_tag="NOA01_000241"
     CDS             3631..4674
                     /gene="hrcA"
                     /locus_tag="NOA01_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HCI0631474.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            4768..5331
                     /gene="grpE"
                     /locus_tag="NOA01_000242"
     CDS             4768..5331
                     /gene="grpE"
                     /locus_tag="NOA01_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCI0631475.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            5382..7211
                     /gene="dnaK"
                     /locus_tag="NOA01_000243"
     CDS             5382..7211
                     /gene="dnaK"
                     /locus_tag="NOA01_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCI0631476.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            7362..8528
                     /gene="dnaJ"
                     /locus_tag="NOA01_000244"
     CDS             7362..8528
                     /gene="dnaJ"
                     /locus_tag="NOA01_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCI0631477.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(8896..9633)
                     /locus_tag="NOA01_000245"
     CDS             complement(8896..9633)
                     /locus_tag="NOA01_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCI0631478.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(9635..11137)
                     /locus_tag="NOA01_000246"
     CDS             complement(9635..11137)
                     /locus_tag="NOA01_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HCI0631479.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            11396..13390
                     /gene="uvrB"
                     /locus_tag="NOA01_000247"
     CDS             11396..13390
                     /gene="uvrB"
                     /locus_tag="NOA01_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HCI0631480.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            13400..16216
                     /gene="uvrA"
                     /locus_tag="NOA01_000248"
     CDS             13400..16216
                     /gene="uvrA"
                     /locus_tag="NOA01_000248"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCI0631481.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            16479..17744
                     /locus_tag="NOA01_000249"
     CDS             16479..17744
                     /locus_tag="NOA01_000249"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HCI0631482.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            17748..18479
                     /locus_tag="NOA01_000250"
     CDS             17748..18479
                     /locus_tag="NOA01_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HCI0631483.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            18614..19498
                     /gene="rapZ"
                     /locus_tag="NOA01_000251"
     CDS             18614..19498
                     /gene="rapZ"
                     /locus_tag="NOA01_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCI0631484.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            19495..20493
                     /locus_tag="NOA01_000252"
     CDS             19495..20493
                     /locus_tag="NOA01_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HCI0631485.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            20527..21462
                     /gene="whiA"
                     /locus_tag="NOA01_000253"
     CDS             20527..21462
                     /gene="whiA"
                     /locus_tag="NOA01_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HCI0631486.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            22030..22686
                     /locus_tag="NOA01_000254"
     CDS             22030..22686
                     /locus_tag="NOA01_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631487.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            22865..23430
                     /locus_tag="NOA01_000255"
                     /pseudo
     CDS             22865..23430
                     /locus_tag="NOA01_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(24129..24650)
                     /locus_tag="NOA01_000256"
     CDS             complement(24129..24650)
                     /locus_tag="NOA01_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCI0631488.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            24848..28528
                     /gene="nifJ"
                     /locus_tag="NOA01_000257"
     CDS             24848..28528
                     /gene="nifJ"
                     /locus_tag="NOA01_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCI0631489.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMTIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            28717..29604
                     /locus_tag="NOA01_000258"
     CDS             28717..29604
                     /locus_tag="NOA01_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HCI0631490.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            29601..30770
                     /locus_tag="NOA01_000259"
     CDS             29601..30770
                     /locus_tag="NOA01_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631491.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            30794..32149
                     /locus_tag="NOA01_000260"
     CDS             30794..32149
                     /locus_tag="NOA01_000260"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCI0631492.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(32221..34026)
                     /gene="glmS"
                     /locus_tag="NOA01_000261"
     CDS             complement(32221..34026)
                     /gene="glmS"
                     /locus_tag="NOA01_000261"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCI0631493.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            34436..34726
                     /locus_tag="NOA01_000262"
     CDS             34436..34726
                     /locus_tag="NOA01_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631494.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            34739..35764
                     /gene="sppA"
                     /locus_tag="NOA01_000263"
     CDS             34739..35764
                     /gene="sppA"
                     /locus_tag="NOA01_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCI0631495.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            35767..36426
                     /locus_tag="NOA01_000264"
     CDS             35767..36426
                     /locus_tag="NOA01_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCI0631496.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            36583..37515
                     /locus_tag="NOA01_000265"
     CDS             36583..37515
                     /locus_tag="NOA01_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631497.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            37669..38958
                     /locus_tag="NOA01_000266"
     CDS             37669..38958
                     /locus_tag="NOA01_000266"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCI0631498.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            39205..40455
                     /gene="clpX"
                     /locus_tag="NOA01_000267"
     CDS             39205..40455
                     /gene="clpX"
                     /locus_tag="NOA01_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HCI0631499.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            40538..41125
                     /locus_tag="NOA01_000268"
     CDS             40538..41125
                     /locus_tag="NOA01_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCI0631500.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(41199..41354)
                     /locus_tag="NOA01_000269"
     CDS             complement(41199..41354)
                     /locus_tag="NOA01_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631501.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            41490..42818
                     /locus_tag="NOA01_000270"
     CDS             41490..42818
                     /locus_tag="NOA01_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631502.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            42829..43038
                     /locus_tag="NOA01_000271"
     CDS             42829..43038
                     /locus_tag="NOA01_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631503.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
BASE COUNT        14282 a         7361 c         9309 g        12141 t
ORIGIN      
        1 gaaagaatta ctaaaaaaat tagatatttc tactggtgta ttagctaatt taattagaaa
       61 tggcatgcca aaagagggag aaatgtttaa tcttgataag attattacat ggcgagaaaa
      121 ttggtctaaa aacatattag gtgagttaga agttggtaga gtatatacta acaaagaaat
      181 ttcagagaag tttaaatgtt ccaaacaggg tggaatgaga agatctcatc aaactaatac
      241 tcttgtactg tttagtgatc aaacaggaag taatgtttat aaagataagt ggttaaatgg
      301 tattttacaa tataccggta tggggctaaa aggggatcaa gtattggata agaatcaaaa
      361 taaagtttta gccaattcaa aaagtaattt tgtaaagatt catttatttg aaacatttaa
      421 accaaaagaa cacacatatc tgggagaagt atatttagca ggacaaatat atactgttaa
      481 tgaaaaagat agttcgggca acagcagaaa agtatataaa tttccattag ctttaataaa
      541 tcaagagcaa ttgattgaag ataaagatat ataaccagga agaaaatcaa actagacata
      601 ttcgtaattt atctaatgct aaattagagg aagaagcaag aaaagttagt aattataata
      661 aaaaaatggc tgcaaaatat gttaacgaag aggagttttg cttctcaaaa acaaaaatat
      721 ataaacgtaa tcccttcgta tctgaatatg ttaaacgact tgcaaaaggt atttgtcaac
      781 tttgccaaga aaaagggcca tttataaaag acggtgtacc ttatttacat tgtcatcata
      841 tagaatattt gtcgcaaggt ggaaaagatg tgattgaaaa ctgtatcgcg ttatgtccca
      901 attgtcatgc tagaattcat gaacttgagt tacctgacga taaagaaaaa ttgctgaaga
      961 tcgttgaaaa aagagaaaat agcttttcta aaataccgta atttattttt ataggacaaa
     1021 taagctaatg tgagctatgc cttctatata agactaagtt ctattataag taacgaagta
     1081 attctcgttc cttcctgtag taatattttt taaataacct ataggcttct tcaaaagtac
     1141 atcctccagt aaattctaag tactggagga ttctttttct agtgttgctt tactcatgaa
     1201 aaagtagttg gctacttcat caaaatttct gaactttttt aatgtataat aaagagctcg
     1261 atcatttact acaaggatgc tagctcctac attttcacag ttttttagca agtaaatttt
     1321 tctaaaatca catcaggaaa tgcatgagat tctttaagag aggcaattat agggctatga
     1381 ttacggttcg tgtaagtgac gccaaaaaag aatggctttc ttatatggct gaattttatg
     1441 ggattacttt atctgaactt ctaaaaaact attcgatgga acaactagaa gatgaataca
     1501 atcgtcaaac ggctgaaatt gcccataaac attggataag aagtgggaaa acaacagtat
     1561 tcatggaaga atccttaatg aatttggtgg gctggaatga cctgccgttt aaaattcacg
     1621 cccgaaattc agaagcagct ctgattggca aatacgtgta ttggtagaga tagaagataa
     1681 tcaattcgtt gttatcgcta ttcatgtagg gcaccgaaga aatgtctatg attaagagac
     1741 tgtttatttt tacaaaaaaa taataattgg aatctagtta aaactcttga agtgagagat
     1801 ttgactagat ttttttatga ataaaataag gacaaaattg tttaggaaga atcatgagtg
     1861 taaaaaaatc atccaagaag tcgattcttg gattttttag tggatttgtt cgccaaatac
     1921 gtttagtatg cgtatatgag aggttgtaag caaagcgttg tggtctgagc acttaaggag
     1981 gaatctgtga aaatagcttt tcatattttt gcagattttg acttaatgcc gaaggtcagc
     2041 tttgcgatta ccgtttatcg agaggctgta aaagcaccgt gtagctccga tgacaataag
     2101 caagcatttt tagagctagg tttccttgat tctaaatatg cggcttattt caatgagttg
     2161 gtgcttgaac gcgattttaa gaaggtcgaa aacaggcacg acttttcgac gtaaggagaa
     2221 agaatttcaa tgatagaaac aataaatccc gcagaaaaca aggtttctgc gtacattcat
     2281 atcccatttt gcgagcatat ctgctattac tgtgacttta ataaagtttt tttagaaggg
     2341 cagccagtag atgagtatgt ggaaatgctg ctgaatgaaa tgagattaat gctggaaagt
     2401 catcctacag cgaatttgga aacactttat gtaggaggcg gaacaccaac ttctttatct
     2461 gctaaacaac ttgatcgact attatcaggt gctagagaga tcctgccttt tcaaaatggt
     2521 aatgagttta ctgtagaagc gaatcctggt gatttgaccc gtgaaaaact tcaagtgatg
     2581 aaaaactatg gcgtcaatcg tttatccatg ggagtgcaga catttgacaa tcgcttattg
     2641 aaaaaaattg gacgtaaaca tactgcagaa gatgtgtatc aaacaatgca atttttagaa
     2701 gcagaaaatt ttaccaatgt aagcatcgat ttgatttatg cattaccggg acaaacacta
     2761 gaaggatatc gggatacttt ggaaaaagct ttggctttgg atctgcctca ctattctttg
     2821 tactcattga tcctcgaaaa caaaacaatg ttcatgaact gggtacgaca aggaagactc
     2881 gaactgcctg atcaggaaac ggaaaccaga atgtttgaag agacgattga agcaatggaa
     2941 aagcatggac gtcaccaata tgaaataagt aactttgggc tctccggaca tgaatctcaa
     3001 cataacctca tgtactggaa caatgaccac tattttgggt ttggagctgg tgctagcggt
     3061 tatcttggag atattcgtta ccgcaacaaa ggaccaatcc aacactattt acgcccatta
     3121 cgagcaaatg aacgcccggt attggaagaa gaaaaattga cacgaaaaaa tcaaatagaa
     3181 gaagaaatgt tcttaggact aagaaaaaaa acagggatta ctaaaaaaca tttctatgat
     3241 cgctatcaaa aaacaattga atcggtgtac ggtgatgtgc taagggaatt agagcaagaa
     3301 gggttacttg ttaatgaaga agaccgtgtt tatttgacac caagagggac ttttttgggg
     3361 aacaatgtgt ttgagcgctt tttatttgaa aaatagtttg aagaatattt gttttttatc
     3421 gaaacttgaa aagaaaaagt ttagaaagtt tgacaatgag gtattgaagt attgatataa
     3481 ctggatttac atcaattatt tagaattagc actcttgtgt ttagagtgct aaaaaatgga
     3541 gtctttctct tgacaggaga agggcttctt gctatattta taagtgaatt agcacttaga
     3601 atatatgagt gctaatgaga ggtgaggaag atgttaacaa agcgacaaaa cgacatttta
     3661 cgtctgatca tccaacacta tacgagtagt ggcgtgcccg ttggttccaa aacattaatg
     3721 tctgagggag tcaaggccag ctcagcaacg attcgcaatg atatgaaagc attagaagat
     3781 gaaggattgc tgttgaagac tcactcttct tctgggcgga ttccatctgc tttaggctat
     3841 cgttattacg tggatcattt gttaaaacct gctcgcgtag cagaagatga tttgctgctg
     3901 attcgtcagt ctctaggcag agaatttcat gaaatcaatg agattattaa gcaatctgct
     3961 gagatattat ctgagctgac aagctatacg actttttcat tgggacctga aataaaagac
     4021 cgccgactga ctggctttcg tatcgtaccc ttgaataatc gacagattat tgctatcgtc
     4081 gtaacagata tggggaatgt agaaagccaa gtgttcactc ttcccgaata tatgggaagt
     4141 caggaactag aaaaaatggt acagattgta aacgaccgtc tagtcggaga tcctctgatg
     4201 acggtgtacc ataaacttcg tacggagatt ccgatgattc ttcataaata tttccagacg
     4261 acagaaggga tgtctcagtt atttgacacc gttcttggtc atgtttttga agaaaaggtt
     4321 tatgtcagtg gacaaatgaa tctcttggat tatgagcccc gtcaagatct tgatcagttc
     4381 aaatcgatgt tttcttttat gaaagactca gacggactta cacaaatgat cactccgatg
     4441 gatagcaata ttcatatcaa gattggtgct gaattaggca atgatttatt gcagaatatg
     4501 agtatgattc aagcaagtta cgagatagat ggtcatggaa gaggtacaat agccttgtta
     4561 gggcctacta gtatgcctta ttctcggatg ttcggtctag tcgatgttta tcgaagagaa
     4621 ctagcagcta aacttgctga ttactaccgc tcgcttgact tgtcaaactc gtaatgtgaa
     4681 taacgaagct atttgcagct gatcgttctc tctttcttag taagagcaat caaacagctg
     4741 actgaataat gaaaggaaga ctgtatcgtg aaagaaaatc aagaagaact agataaagaa
     4801 atgacagatg ctcagccaga accggaaatc gatgtcgaag ctgcagaaga ttcgggtatt
     4861 tcagaagctg aagcggaaga attcgaaaca gcgaaattaa aagctgagtt agaagaaatg
     4921 gaagataaat acttgcgggc tcgtgctgaa atcgcaaata tggcaaatcg aggaaaaaat
     4981 gagcgtgagc aattacaaaa ataccgttca caagatttag ccaaaaaatt acttccatca
     5041 attgacaacc tggaacgtgc attagccaca gaagtgtcag atgatcaagg ggcaggtttg
     5101 aaaaaaggcg tggaaatggt cttggaaagt ttgcgtaacg cactggaaga agaaggaatt
     5161 gaaaaaatcc ctgctaaagg agaagccttt gatcctaatc ttcatcaagc agtacaaact
     5221 gttccagcga ctgaagatac accagccgat acgattgtag aagtactaca agaaggctac
     5281 aaattacatg atcgcgtgtt aagaccaact atggtcattg ttgcacaata attgtgcgga
     5341 tataaaactt acactttcaa ttaaagaagg agacgttaat tatgagtaaa attatcggta
     5401 ttgacttagg tacgactaac tctgcagtag cagtattgga aggaaacgaa gcaaaaatca
     5461 tcgctaaccc agaaggaaac cgtacaacac cttctgttgt atcattcaaa aatggagaaa
     5521 tccaagtagg ggaagttgca aaacgtcaag ctgtgacaaa cccaaatacg atttcatcaa
     5581 tcaaacgcca tatcggtgaa gcaggttaca aagtagatgt agaaggtaaa tcatatacac
     5641 cacaagaaat ctcagcaatg atccttcaat atatcaaagg attcgcagaa gactatttag
     5701 gtgaaaaagt cgaaaaagca gttatcactg tgccagctta ctttaatgat gcacaacgtc
     5761 aagcaacaaa agatgctggt aagatcgctg gtctagaagt agaacgtatc gtcaatgaac
     5821 caactgcagc tgctcttgcc tatggtttag ataaaacaga tcgcgacgaa aaaatcttag
     5881 tattcgacct tggtggcggt acttttgacg tatctatcct tgaactagga gacggtgtct
     5941 ttgatgtatt gtcaacagct ggcgacaacc atctaggtgg ggacgacttt gataacaaaa
     6001 tcatcgacta catggtagca gaattcaaaa aagaaaacag cattgacttg tctcaagaca
     6061 aaatggcttt acaacgcttg aaagacgctg ctgaaaaagc gaaaaaagat ttatctggcg
     6121 tatcaagcac acaaatcagc ttgccattta tcacagcagg agaatctgga ccattgcatt
     6181 tggaaatgac attgacacgt gcaaaatttg atgaattaac agctgactta gtagaacgta
     6241 caaaagtacc agtacgtcaa gcgttaaaag atgcaggtct gtcacaatca gaaatcgacg
     6301 aagttatttt agtcggtgga tctactcgta tccctgcagt tgtagaagca gtacgtaaag
     6361 aaacaggaaa agaaccaaac aaatcagtta accctgatga agtagttgct atgggtgctg
     6421 ctatccaagg tggcgtgatc actggtgatg tgaaagatgt tgttttactt gacgttacac
     6481 cattgtcatt aggtatcgaa acaatgggtg gagtgttcac taaattgatt gaccgcaata
     6541 caacgattcc aacaagtaaa tcacaagtat tctcaactgc ggcagataat caaccagcag
     6601 tagacatcca tgtattacaa ggtgaacgcc caatggctgc tgacaacaaa actctaggaa
     6661 gattccaatt gacagatatt ccagcagctc ctcgtggtat tccacaaatc gaagtaacat
     6721 tcgatattga taaaaacggg attgtaaatg tatctgcgaa agatctaggt acacaaaaag
     6781 aacaaaaaat cacgatcaaa tcttcttcag gtctaacaga cgaagaaatc gaacgtatgg
     6841 tgaaagatgc agaagcaaac gctgaagcag ataaagctcg taaagaggaa gttgacctac
     6901 gtaatgatgt agacgcattg ctattctctg tggacaaaac attgaaagaa ttagaaggca
     6961 aagtggacga agaagaagtg aaaaaagcag aaacagctcg tgacgaatta aaagccgctg
     7021 ttgaagcaaa caacatcgag gaaatgaaaa ctaaacgcga tgcattgaac gaaatcgttc
     7081 aaaacttgac tgttaaacta tacgaacaag ctgcacaaca acaagctcaa gaaaatccag
     7141 aagcagctca aggcggagca gatgatgtcg ttgatgctga ttttgaagaa gtcgatggcg
     7201 acgataaata atcagttcac tgaatttcga gagaaggcca aagttgctac tttggctttt
     7261 tcttttgaaa aatagtttga agtatcggtt tttgtttcaa acaaaatatg atatgataac
     7321 aagcgacgtg aaaatgatga gaaccaatag gaggaaagcc tatggcaaca aaacgtgatt
     7381 attatgaagt cttagggctc tcaaaaggag catcagaaga tgaaataaag aaagcttatc
     7441 gtaagctttc gaaaaaatac catcctgaca tcaataaaga acctgatgcg gaagaaaaat
     7501 tcaaagaagt atcagaagca tatgagattt taagtgaccc gcaaaaacgc gctgcatacg
     7561 accaatacgg gcatgcaggc acagatccaa attacggtgc tggtggagct ggtggcggat
     7621 ttggtggatt cggcggcttc tctggtggcg gcttcggtgg ttttgaagat atcttcgact
     7681 ctttctttgg aggaggcggt cggtcagtcg atccaaatgc gcctcgtcaa ggtgctgatt
     7741 tgcaatatac ggtcgacttg tcttttgaag aagcgatttt tggtgtagaa aaagaaatca
     7801 agtataatcg ggaagaaatc tgtcatactt gtggtggaaa cggtgcaaaa ccaggcacac
     7861 aaccaactac ttgtcataaa tgtcatggtg caggtacgat caatgtagaa cgccaaacgc
     7921 cacttggacg agtgatgagc cgtcaaactt gtgatgtttg tcatggcaca gggaaagaaa
     7981 tcaaagaacc ttgtccaacc tgtcatggct caggacatga gaagaaagca cattcagtta
     8041 aagtgaatgt accagctggt gtagaagaag gacaacaaat gcgtttagct ggtcaaggag
     8101 aagctggaga aaacggcgga ccatttggtg atttgtatgt ggtttttcgt gtagaagaaa
     8161 gtgatatctt tgatcgtgaa ggttctgaaa tttactatga attgccttta agttttgtac
     8221 aagcagcttt gggggatgaa gtccaagtac caactgtcca tggagatgtg aaattgaaga
     8281 tccctgctgg aacacagaca ggtacgaact tccgtttacg tggaaaaggc gctccgaagt
     8341 tacgtggtgg tgcaacaggc gaccaacatg tcaaagtaaa actgatcaca ccgaaaaatc
     8401 taaatgaaca acaaagagaa gcattacgtg cattcgctga agctggcggc atcaaagttg
     8461 aagaacagca agaagacggt ttcttcgata agtttaaaga tgcttttggc gggaaaaagc
     8521 ggaagtagtt tttctagtaa aaatataaag tagacttgcc caaattttct atttctagta
     8581 aaaacgctaa gtcattaata attaaaaacc agacgaacaa tcatacgttc gcctggtttt
     8641 tttgtagttg tcatgtttgt accaacaaaa aagccagctt tagaataaga taatcatcat
     8701 agtttctgaa ggtggtatcg actatgtact tgatatcgtc aatgaaccga tggtactttc
     8761 ttatgcgatg gaacaggtag agcgattgct tgtttgtgca ggagaacaag cgatgcggct
     8821 ggtacagtta agtgaaaaaa caagaaacta aaaatgcccg gtcgtcatga agcgaccggg
     8881 catttttggt ttttattaaa tatttaatac tttattcaag aaatcttttg tccgttcatt
     8941 ttgcggattt tcaaagattt gttgaggcgt tccgtcttct aaaaagtttc cgtcgtcaat
     9001 gaacatgaca cgattggcca cttctttagc gaagcccatt tcgtgcgtaa cgataaccat
     9061 cgtcatacct tgttctgcta actttttcat gacgttcaag acatcaccga ccatttcggg
     9121 gtcgagtgca gatgtaggct catcaaaaag catgatgtca gggttcatcg ctagagcacg
     9181 agcaatagcc acacgctgct tttgtccacc ggaaagcatt tcaggataca tatctttttt
     9241 atcttctaga ccaactgtct gcagcaattt gattgctttt ttctctgctt cttctctaga
     9301 ttctttcttc aggtccatcg gtgctaaaat gatgttttcc aaaatagaaa gatgagggaa
     9361 taggttaaag tgctggaata ccattccgat atgttgtctc accaagttga tatctgtgct
     9421 tttatccatc aagtgggcgc cgtcaataat aatttcaccg tcagaaggtt cttctaaacg
     9481 attcaagcaa cgcaaaaatg tactttttcc tgaacctgaa gggccaatga tacagacgac
     9541 atctccttcg ttgatggaca cattgatgtc gtttaatact gtgttatcgc cgtatttttt
     9601 gactaaatgc tcaactaaga ttttttctgc catcttactt caccttcttt tctaaaactt
     9661 ttgctaattt tgtcaatgct gtgatcaaaa tcaagtacat gatcgcaatg atcaagtaaa
     9721 catacgtact ttgtgttgta cgtgcaacaa tgatcttacc agtttgcagc aattcgacta
     9781 caccgattgc ggaaatgatc gttgtgtctt ttaatgagat aacgaactga ttgacgaatg
     9841 atgggatcat gatgcgtatc gcttgcggca aaatgatttt ttgcattgtc cgattataag
     9901 aaagacctaa acttctggaa gcttccattt gtccgacagg aacggcgttg atcccgcctc
     9961 gaacaatctc tgcgatataa gcacttgcgt taagagtcaa ggtgataaca ccagcaagga
    10021 agtcaggaat cgtaaatcca acgataccag gcaacccaaa gaaaataaag aacgcaagaa
    10081 ccatcattgg aattcctcgg atgatatcga cataaatact agcaattgtg cgaagagcgc
    10141 gtattggtgc tacactgaat aatccaaaaa tcacaccgat tactatagcc aaagcaaagg
    10201 agatcaaagt taggagaatt gttctccaaa gtcctttcaa caatgcttga tagttgtttt
    10261 ggataagtcc aagaaatgtt gattcatcag cagtggaaga ctcactggaa gtttctgaac
    10321 catccgctac atattcttta ataatcttat cgtattcgcc tgttcttttt aattctttca
    10381 atccttcatt gaacatatta agaagctcag gattttgtcc ttttttaacg gcaaatgcat
    10441 attttccgcc agattctttt ttgatcggtg tttccaattc ttgtccttgt ttgatcgcat
    10501 aaccgattac cggataatca tccatcattg catcgattgc gccaactttt aacccgtcgt
    10561 acaattgatc agcagcatca aaatatttga tagaatagcc atattcagat tcatggtcgc
    10621 ttaaaaattc agcactttcg gtaccaactt ttgctccgac tgtttttcct ttcaaatctt
    10681 cataagactt gatcgaagta tctccttttt tgacagctaa ttggatcccg ctatcaaagt
    10741 aagaatcaga aaaatcatag cttttctttc gttcatctgt tacagtcatt ccggcaatca
    10801 ttccgtctgc ttgtcctgat tccaaagctt gcaaagcagc agagaaacca cggtagtcca
    10861 ttcggatgtt aaatccttgc agtttggcga tgttttccat caattttaca tcgatgcctt
    10921 cgtactcgcc ctttgcgttt tgaaattcaa atggagcaaa agcagagtca ctggcaatta
    10981 cgtatacgtc ttttttgggc tctatttttt gcattgtctc gcttgtgctt gactctgccg
    11041 ttgtagtgtt gtcagcttgt gcaacatgtc caaacgcaag tgtcacaagt cctgtcatta
    11101 agaaaacaaa tgagtgaaat aaatgctttc ttttcataat caacctccta aatttccaat
    11161 attttattat tataggcgaa aagtgctgaa tcagacaaga ctgtaagctc gaaaaaatga
    11221 aaaaaatgcg agtttctaac attttatgtt ataaaacgta acatgcataa taagataaaa
    11281 aagtggcgaa agtatgttcg gtgtggtata attttttagt aaggaatgcc tttgctttcc
    11341 ttttcttttt attggtcgta aaatgaaaag gaatgaatga aaaggaggac gaagaatgat
    11401 tgagagagat acctcccgac actttgagct tgtctcgaaa taccaaccgg ctggtgatca
    11461 gcctgaagcg attaaccaat tagtggatgg cgtagtcggc gggaaaaaag cacaaatctt
    11521 attaggtgcg actggaacag ggaaaacata tacgatttcc aatcttattg agaaagtgaa
    11581 taaaccaaca ctaattattg cccataacaa aacattggct ggtcagctgt acggggaatt
    11641 caaggaattt ttccctaaca atgctgttga atattttgtt tcttattatg attattatca
    11701 gcctgaagct tatgtgcctt caagcgatac gtatatcgaa aaagattcaa gtgtaaatga
    11761 tgagatcgac aaattacgac attcggctac tagttcatta ttagaaagaa atgatgtgat
    11821 cgtcattgca tccgtttcat gtatttttgg attaggttcg ccttttgagt accaaaaaca
    11881 agtagtttcg attcgacagg gagcagaact agatcgcaat cagttgattc gtgacttagt
    11941 cagtatccaa ttcgaaagaa atgatataga ttttcagcgc ggtcgttttc gagtgcgcgg
    12001 tgatgtcgtg gagatttttc cggcttcaag agatgaacgt gccttacgtg tggagttttt
    12061 tggagatgag gtcgaacgta tccgagaagt caatgcactg acaggtgaag ttttaggaga
    12121 aacggaacac gtggcaatct tcccggcgac tcactttgtg acgaatgatg aacatatgga
    12181 gcatgccgta gcgaatatca aagcggaatt agaacagcgt ttaactgtct tgcgaaatga
    12241 aaacaaactt ctggaagctc aacgtttaga acagcgaacc aattacgata ttgaaatgat
    12301 gttagaaatg ggctacacgt cgggaattga aaattattct cggcatatgg acggacgtaa
    12361 agaaggtgaa ccgccttata cgctattgga tttctttcct gaagattttt tgatcgtggc
    12421 ggacgaatcg cacgtaacca tgcctcaagt cagaggaatg tataatggtg accgagcgag
    12481 aaaacagatg ctagtagatt acggtttccg tttgccatct gctttagaca atcggccact
    12541 tcggttagaa gagtttgaaa aacatgtcaa tcagatcatt tatgtctctg cgacgccagg
    12601 accatatgaa catgaacaaa cagataccgt tatccaacaa atcatccgtc cgacagggct
    12661 gcttgaccca gtgatcgaag ttcgtccaat catgggacaa atcgatgatc tagtaggcga
    12721 aataaatgaa cgagtcgaaa aagaccagcg ggtgtttgta acgacattga cgaaaaaaat
    12781 ggcagaagac ctcacagatt atttcaaaga attagggatc aaagtcaaat atttgcatag
    12841 tgatatcaaa acgctggaac gaacagaaat cattcgagat cttcgattag gtgaatttga
    12901 tgtgttagtt ggaatcaatt tgcttcgtga aggattagac gtacctgaag tttcccttgt
    12961 tgctatcttg gatgcggata aagaaggctt tttacgtagt gaacgttcct tagtccaaac
    13021 gattggacga gctgctcgga atgaagaagg taaagtaatc atgtacgcag acaaagtaac
    13081 agattctatg cgtctagcga tggatgaaac atcaagacgc cgtacgattc agcagaagta
    13141 caatgaagaa catgggatcg ttccaaaaac gatcattaaa gaaatccgag atttgatttc
    13201 tattacgaaa gaatcggaag atgatacaaa agaagcggta caagtctcgt atgaagaaat
    13261 gacgaaagaa gaaaaagata cgttattgat gaaattagaa aaagaaatga aaaatgcggc
    13321 gaaagcattg gattttgaaa cagccgctaa tgtgcgagat atgattttag aattaaaagc
    13381 ttcaaagtag gaggacaaca tggctaacga taaaattacg attcacggtg cacgtgccca
    13441 taacttgaaa aatatagatg taacgattcc acgagataaa ttcgtggtcg ttacaggttt
    13501 gtccggttct ggaaaaagtt ccctagcatt tgatacgctg tacgctgaag ggcagcggag
    13561 atacgtagag agcctttctg cttatgctcg tcagttttta ggacaaatgg ataagccgga
    13621 cgtagatagc atcgacggat tgagtccggc gatttctatt gatcagaaaa caacaagtaa
    13681 aaatcctcgg tcgacagtag gtactgtgac ggaaatcaat gactatttac gcttgttgtt
    13741 tgcacgtgta ggacatccga tttgtcccaa cgatcatatc gagattacca gtcaatcagt
    13801 agatcaaatg gcagatcaag taatggcttt gcctgagcgc acaaagatcc aagtgttggc
    13861 accgatcgtg gtaaagaaaa aaggacaaca taaaaaaata ttcgaacgaa tccaaaaaga
    13921 gggatacgtc cgcgttcgag tcgatggtga gatctatgat ttaagtgaag tgcctgagct
    13981 ggagaaaaac aaaaaacatg atatcgcgat cgtcatcgac cggatcattg tgaaagaagg
    14041 gattcgttct cgtctatttg attctttaga ggcggctctg cgattggcag acggctatgc
    14101 tctggttgac gtgatcggtg aagaagaaat gttgtttagc gaacactatg cctgtcctta
    14161 ttgcggattt actgttggcg aactagaacc aagactgttc tcattcaatg cgccttttgg
    14221 agcttgtcca gactgtgatg gtttaggtgt caaattagaa gtcgatcaag atttagtgat
    14281 tccagataga acgaagacac ttagtcaagg tgctatcgtt ccatggaatc caatcagctc
    14341 tcagtattat cctcaaatgt tagcgcaggc ttgtgaaagt ttcggtatcg atttagatac
    14401 accattcgaa gaattaccgg aagagcatca ggatatcatc ttgaatggga cagatgatct
    14461 tttccatttc cattatgaaa atgattttgg tggcgttcgc gatgtagaaa cgactttcga
    14521 aggggtattg aagaatatcg agcgtcgtta tcatgatacg aacagtgatt tcacgcgtga
    14581 gcaaatgcgt ctttatatga cagaattaac ttgtcagaca tgtaaaggtt atcgattgaa
    14641 tccccaagct ctatctgttc aaatcaatgg aacgaatatc gggcaaacaa atgagttgtc
    14701 tattcaaaaa gcagtagatt tcttctcaaa cttaacgtta tctgaacaag agaaagtaat
    14761 tgccaaaccg attttaaaag aagtaaatga cagactttca ttcttggaaa atgtgggact
    14821 agactatcta actttgagtc gcgcttccgg aacattatct gggggagaag cgcagcgaat
    14881 cagattggct acacaaattg gatcgaatct ttctggagtt ttgtatattt tagatgaacc
    14941 atcgatcgga ttgcatcaac gcgacaacga tcgactgatt ggctcattaa agaaaatgcg
    15001 tgatcttggc aacaccctcg ttgtagtcga acatgatgaa gatacgatgt gggcagcaga
    15061 ttatctgatt gacgtcggac ctggggccgg agaacaaggc ggacaaatcg tggctgcagg
    15121 cactcctgaa gaagtagcta atgatgaaaa ttctttgaca gggaaatatc tttcagggaa
    15181 aaaatcaatc cctgtaccaa aagaacgccg taaaggtaat gggaaagcaa tcaagatcac
    15241 tggtgcttca gaaaacaatc tgaaaaatat tagtgttgaa tttccattag gagaatttgt
    15301 agcagtcaca ggtgtgtctg gttctggaaa gagtacattg gtcaatagta tcttaaagaa
    15361 atcgttagcg caaaaattaa ataagaattc tgctaagcca ggtaaattca agacaatttc
    15421 cggctacgaa agtatcgaaa agatcatcga tatcgatcaa agcccaatcg gccggacgcc
    15481 gagaagtaat ccagcgactt atacaagtgt atttgatgat atccgtgggt tatttgctca
    15541 aacgaacgag gcaaaaatgc ggggttataa gaaagggcgt tttagtttca acgtaaaagg
    15601 cggtcgttgt gaagcttgtc gcggggatgg aattattaag atcgaaatgc actttttgcc
    15661 tgatgtctat gttccttgtg aagtatgtca tggcaaacga tataactctg aaacattaga
    15721 agtgcattac aaaggaaaaa gcattgctga tattttggaa atgacagtag aagatgctgt
    15781 agaattcttc aagcacattc caaagattca tcgcaaactg caaacgattg ttgatgttgg
    15841 cttaggttat gtgacgatgg ggcaaccagc aacgacattg tccggtggtg aggcacaacg
    15901 gatgaaactt gccagtgaat tgcacaaaat ctctaatgga aagaatttct atatactaga
    15961 tgaaccaacg acaggacttc atagcgatga catcgcccgc ttgttgcatg tattacaaag
    16021 attagtagat gctggtaaca cagttttagt gattgaacac aatctagatg taatcaaaac
    16081 agcagattat atcattgatt taggaccaga aggtggagaa ggtggaggaa cgatccttgc
    16141 gactggaaca ccagaagaaa tcattaacgt aaaagaaagt tatacaggtc actatttgaa
    16201 aaaaataatg gtataaagag gatagatagc tgagatagtt aaaaaaacat aaaaaaatga
    16261 atttttgctt gttttattaa tatcagtgtt ctagaatcat tacatggtag ttctttctac
    16321 actgattggt atagacaaaa acttcctata agttcaatcc gttcaaaaaa gaattgagca
    16381 atcggaggag tttcctttga ttgctttttt ctttatggta tgatattcct tgtgtagttg
    16441 gaaaagaaaa acaagagatt atgagtaagt gagagataat gatgaacagt attgaaaatg
    16501 aagaatttat ccctatatta ttaggaagcg acatgaacgt ttatggtatg gcacgttctt
    16561 ttaatgaagc atacggcaaa atctgccaag catatgcttc ggatcagtta gcccctaccc
    16621 gctacagtaa aattgtcaat gtagaagtta ttccaggatt tgataaagac cctgtgttta
    16681 tcgagacgat gcttcgtttg gcaaaagaaa gatattctga taagagtaaa aaatatcttt
    16741 tgattgcttg tggtgatgga tatgcagaat tgatctccca acataaacaa gagctgtcag
    16801 aatattttat ctgtccatat attgattatt ctttatttga aagactgatc aataaagtaa
    16861 gcttttatga agtttgtgaa gaatatgatc ttccgtatcc taaaacattg atcgtcagag
    16921 aagaaatgtt agtaaatgga catttggaac aggaattgcc ttttgaattc ccagttgctt
    16981 tgaaacctgc aaacagtgtg gagtatcttt ctgtccaatt tgaaggaaga aaaaaagctt
    17041 ttattttaga gactagagaa gagttcgatt taattttagg ccggatatac gaagccggtt
    17101 ataaaagtga aatgattgta caagatttta tcccgggtga tgatagtaat atgcgtgtgc
    17161 taaatgcgta tgtagatgaa gatcatcaag ttcggatgat gtgtcttggt catccactgt
    17221 tagaagatcc tacaccagca tcgattggta attacgttgt gatcatgcca gattataacg
    17281 aaaaaatcta tcaaaccatt aaggcatttt tggaaaaaat cgaatataca ggctttgcca
    17341 atttcgatat gaagtatgat cctagagatg gagaatataa attatttgaa atcaatttga
    17401 gacaaggtcg tagcagtttc tttgtgacat tgaacggctt gaatctggct cgtttcgtca
    17461 cagaagatcg agtgtttaac aaaccctttg ttgaaacgac ctatggaacg aatcaatcag
    17521 ataaagcaag attatggatg ggtgtgccca aaaaaatctt tttagaatat gctcgggaaa
    17581 atgaagacaa aaaactagct gagcaaatga tcaaagaaaa tcgttacggt actactgtgt
    17641 tttatgaaaa agaccggtca atcaagcgct ggttgttgat gaaatatatg ttccataact
    17701 atattccaag attcaaaaag tattttcatg tgaaagaagg ctagttcatg gagaattttt
    17761 tcagtatttt aggcggaatg ggcacgatgg cgacagaaag ttttgttcgc ttgatcaacc
    17821 accgaacgaa agctacaaaa gatcaagaat atttgaatta tgtcttattc aatcatgcaa
    17881 cagttcctga tcgtacagct tatattttag atcgatcaga agaaaatcca atgccgtttc
    17941 tgctggatga tattgagaaa caaaatttat tgcagccgaa cttcattgtg ttgacttgta
    18001 acaccgcaca ctactttttt gaagaattac aagcggcaac ggatattcct atccttcata
    18061 tgccaagaga agcagcaaat gaacttgtcc gtcagcatac gacagggaga gtagcgattc
    18121 taggaacaga aggaagtatg aaagctggaa tctatgaacg cgaagtcaaa aatcttggat
    18181 tcgaaacagt gattcctgat actgttcttc aagaaaaaat caattatctg atctatcatg
    18241 agatcaagga atcggattat ttgaatcaag agctatatta cgaaatctta gaagaagcgg
    18301 tagagcgcct aaactgtgaa aaagttatct taggttgcac agaattgtca ttgatgcatg
    18361 aatttgctga agataaccat tatccagtga tcgatgccca atctatttta gcagatcgga
    18421 cgattgaacg agcgctagct gaaagaagca aggcattgga tacagcatcg gaaaagtaaa
    18481 aaaattggat ttggggcaaa tatcctaaaa gatatttggt ccaaatcttt ttgtaaaaga
    18541 aaaagaaaac tggtaatgat acgcccggaa tgatataatt ttactatgca caatgtgaga
    18601 ggagttcgta attatgacag ataatttgca attagtcatc ataacaggta tgagcggtgc
    18661 agggaaaaca gtagctatcc aaagttttga agatatgggc tatttttgta tcgataatat
    18721 gccgcctcga ttgattccaa aattctggga attaatcaaa gaatcaggca aagtcacaaa
    18781 aatggcttta ggagtggatt tacgttctcg ttccttcttt gaagaaatcc aaaacatgct
    18841 gatcgaaatc gaaaatacga acttcatcga cacttctatc ttatttttag atgcttcaga
    18901 cgaagaacta gtggctcgct acaaagaaac aagacgagca catccaatgg cgatggacgg
    18961 tttgatcact gaagggatac gtaaagaacg ggcgatacta gacgacttga aagcccaagc
    19021 ttccgtgatc attgatacga cgacattgac accacgacaa ttacgtgaaa aaatcaatga
    19081 gtcattcaaa tcaaagggag accaaggatt tcggatcgaa tttgtatcat ttggttttaa
    19141 atacgggttg ccgatcgatt cggatattgt gatggatgtg cgttttttgc ccaaccctca
    19201 ttatatcccg gaactaagac cattgaccgg aaaagatcag ccagtctatg attatgtgat
    19261 gtcctttgat gaaacgcaat cattctacca taaatttttg gatcttctgg aaactgtatt
    19321 gcctggatac attaaagaag gaaaaagcag cttgacagtt gcaatcggct gtacaggcgg
    19381 tcaacaccgt tctgtggcat tgactgaacg tttggcaaat gctttaggga aaaactataa
    19441 agtcaatgtg acacatcggg acaaggataa aaggaaagag acggtgaacc ggtcgtgaaa
    19501 atgaaaacct accggataag aaaaccaaag atcgtagtgg tcggcggtgg cactggtctg
    19561 ccagttatcc tgaaaagttt gcgaaatcaa ggagcagata tcacagcagt agtgactgtt
    19621 gcagatgatg ggggaagcag cggtgcaatc agagaatcga tcgctatggc tcctcctggt
    19681 gacttgagaa atgtcttagt ggcgttatcg gatatgccac aattctatga agatattttc
    19741 caataccgtt tcaaaaagga agatcagttt ttagccaatc atacgatcgg taatctgatc
    19801 attgccgcag tttccgagat gcggggaagc acttatgagg cgattcaatt attagcaaaa
    19861 atgatgcatg tagatggaca tgtttatcct tcttcagaaa ctcctttgac cttgcatgct
    19921 gtctttaaag acggaacgac tgcagtagga gaatctaaaa tcgcgatcga ccgtaaaacc
    19981 attgaccgtg tgtatgtctg caacacaaat gataaaagtg aagccaaagc ggctcgaaaa
    20041 gtcgtttcag caattatgga cgccgacatg gtcgttttgg gacccggaag tttattcacc
    20101 agtatccttc caaatttagt gataccagaa atcggcgaag caatgctcaa gacaactgct
    20161 gaaaccgtct atatctgtaa tatcatgacc caaaaagggg aaacggaaca tttcacagat
    20221 gccgatcata tacgtgtttt acacaagcat ttgaatcagc cttttatcga tacagtattg
    20281 gtcaatacgg aaaaagtgcc tgaagattat atggacccag agatctatga tgagtatcta
    20341 gtacaagtcg cacacgactt caacggattg agaaatgaag gatgtcgagt gatttccacc
    20401 gactttttgg aattgaaaga tggtggtgtc ttccacgatg gagacaaagt agtagaagaa
    20461 ttgttccgtt tagttttcgg ttcaaaatat taagcaattt attgttggag aaaggaggag
    20521 aataggatgt catttgctgc agatgtgaag aaagaactaa ctggtttgga agttcatcga
    20581 gaacatgcga aagcggaatt agctgctttg ctgcgaatga acggctcttt aggtattgtg
    20641 aaccagcagt ttattttgaa cgtccaaaca gaaaatgctg caattgccag acgtatctat
    20701 tctcttttga aagatcatta tcaggtacga agtgagctac tcgttcgtaa aaaaatgaaa
    20761 ttaaaaaaga acaatgtcta tatcgtccgg ttgaagcaag gcacaaaaga tgtgttgatg
    20821 gatcttgata tcatggatgg tatgatgttc aatggacacg tttcggacga gattatggga
    20881 aattcgcaaa aaatgcgttc ttacttacgt ggtgctttta tggcctcagg ttcagtcaat
    20941 aatccggaaa cgagccgtta tcatttggaa atattttcta tttatgaaca gcataaccaa
    21001 gacatttgcg atatgttaaa ttattacggt ctacatgctc gaacgcttga acggcgcaat
    21061 ggctatattt cttatttaaa aggagcagaa aaaattgctg atttcttaac attgattggt
    21121 gcaaccaact caatgctccg atttgaagat gttcgaatcg tacgggatat gagaaattcg
    21181 gtcaatcgtc tagtcaattg tgagacagca aatctgaaca agacgattga tgcggcttct
    21241 aaacaaatcg aaaacatcga attgatcgaa gcaaaggtcg gtctacatgc ccttcctgaa
    21301 aaacttcagg aaatcgcaga acttcggctt caacatccag aggtcagctt gaaagaatta
    21361 ggggaaatga ttccctcagg tgcgatatca aaatcgggaa ttaatcaccg aattcgcaaa
    21421 ataaatgaat ttgcggaaaa actgcgtgaa gaagcagttt aatccaaaga aaaaactgat
    21481 acatgaagca ggagatgcga atgtatcagt tttttttaaa agttatactt gcttttcttc
    21541 cgtcaaacta taattccatc tttactggag taacataatt tggaaaggct tctattgttt
    21601 ttaatacaat cgtataaaaa gcggggagac aactgaatga agattttagt tttgtacata
    21661 aatttttgct taatatagaa gtcctgatat agataagatc aagtatttat ctataactgg
    21721 cctgattaat taaaaaactg gaaggaactg ctcgatcatc cttgagaact gctcttctta
    21781 cttctggtca agattctcgt ggttggtgga taaggatggt tgataagttc attggattag
    21841 ctagtcgaaa acaaccaggt gtaattaatg tagaagtttt aacaagggga tggagtgatt
    21901 tattcagtga taatcataag ttcataaata gtaattgaga tgtatggttg gaatacgtta
    21961 tacctgaact tgaactacca agatttggtt tagaatttga gaatcgatgt ttataaaggg
    22021 agggcaaaaa tgcgtttttt tacaactttt actatcatta tgattgcagt actttttatt
    22081 tttctagata tagcaaagcg aaatacagca tttttattat atcgggtttt acttcgagca
    22141 ggtctcataa catttatatc gatcgttgga ttttttctat tcacagttat tgtatttatt
    22201 tggagaactc cagcaccacc attgccagag attacatatg gagaatttcc ttttagactt
    22261 gagtatgaat tgaatgaaga attacatgtg atagaagaca cgttaatagt tgaatttgat
    22321 ggatttggaa tgaacgaagg catagggaga tatcgtagat ggacaagtcg tttagccagc
    22381 ggagaagatt tagtgttgct gttagaagtt agtgataata agcaaatatt ttattttcca
    22441 ggacctgcta attattacat gggagatagg ttgaatggtt ataatcatac ttttcctagt
    22501 gcttctttca ttgaaaggga aagaggcatt actagaaggg atattttaca tgacaaagaa
    22561 ttattagaac aatttggtcc gcttgatcaa aatacaataa atgaagaaga attgttaaat
    22621 caatataata taaggctagt gaattgggaa atatctgaac cgattgttaa caattttggt
    22681 gattagaaaa caattacgat aaatagaaac aagccaaatc atcagcaagt aaaagctgat
    22741 gatttggcct ttcttatacg agtagtagga attcatagcg aatattatta cgatcagtag
    22801 atattttgat attaaaggag tattacgaac tagtatgtga gaatgtagag gtgatttgcg
    22861 aataatgaga aatttatcaa atagaaacaa aatattaatc attattgttg ttatagctgt
    22921 gtttcatctg ggaacgaatg cggttttaag tagaataata ttgggtccta agccaccaag
    22981 acctgcgatt acccgagggg agtttgattt tagattagaa tatgaagtag atggggaaag
    23041 gattgtgata gaagatacaa ttgttgcatt atttgatgga ttttctgcag atgcaggtag
    23101 catggcatgg tatcgcacgt ggcgtttgca tttggcgagt gacaagaggg cacaacaatt
    23161 attgttacat gagttagatg atggtcgaaa aatatattat cattttggag gagctggatt
    23221 ttatatgggg aagttagtag cagtgtaact gtaacaagga aagatttgcc aggtcttggc
    23281 attcgaaact ccatatttta tatcgagcgt gggagagata catctattgg gaacaggaaa
    23341 gggttaactc ttgaagagtt atataatgac tttggaatcc gtcttgttag ttgggaacac
    23401 aatccaccaa tagaaaatcg ctttgaataa tcgaaatcta ttaatccatt tcatcagcca
    23461 gtgaatgttg taatggaaca atttggttta gtgttcaaaa ccattgaaat actaggctgt
    23521 gatcagcaaa aatgtatctg aataaaaaag agagcaacta ttaaatgaga ggagttagga
    23581 gttgttagct ttatacgaat caaagtggca aatacagatg cgtatgcatt tgtgtttgcc
    23641 atcttgcgtt tggtagacga gataggtata agctaaagaa acaacttgtt taaaataaac
    23701 gacctagctc aacaaacgac catctaatca actaacagca tacataaaag caactatttt
    23761 tgaagtagaa ttgaaatcgg gataacttaa aaagtgattg ggatactagt gtgaatcacc
    23821 gtacataaat attactcgtt aagggaattt gagaaatttt gtatgaaaat ttaagaatta
    23881 gttagtatgc gcaagtcaaa aatatgaata aattatttgg tggagataat ttgctcagct
    23941 tgtcataaga aatgagataa tctggcagat gcaagctttt ggacaccatt tattcggaat
    24001 aaggaggctg ggacagaagc gtttaactcc aagaaataag aaggagttgc ttctgcttcc
    24061 gccgtttata ggtgtttaga gcgtgagaca aaagtgttct ttacttttgt cctacgctcc
    24121 ttattttttt attttgtttg gatcaattct tttaattcct ctattgaaat agattcatca
    24181 aagatttctt tacccaagat gacagtcgga acaaatcgaa tatgtgcttg ctcagcttcg
    24241 ttgatgacag cttggctcag ctgttcgtct ttttgttctg tcagctttag tttatctact
    24301 gcaaaatccg ctacttcttg gagagaaaga tgtttccact cgtcttgagt atcgaatatt
    24361 tgtttgatct ctttgatcgc tttttgacca tcgctgattg tgagataccg gtgcatcacg
    24421 tttcctcgct gcaagctttc tttttcttta tcgaacaatt tgatcactcg ctgcaattgc
    24481 ccgctttgga ctgcttcttc taatagctca taagattctt caaaccattg tcggcaatac
    24541 ggacaagata ggttgatgaa ttcgaccatt tttttaggag cattcgatga accataaagg
    24601 attccttttt gtgtatttgt tttcgttgca tcaatcactg atatatccat gttaaaacac
    24661 ctctttcgct tatagtttat acatgtctga caaaaaaagg caacgattta gactgtttgt
    24721 ctaaaaatta gatgaccaat tcaaatagac atatgtgaaa tatttttata aataaaagaa
    24781 aaaatggaat gttttacgtt ataatggaac agataataag agagaatatg aacaaggatg
    24841 atgaatcatg cgaaaaatga aaacaatgga tggcaatacg gcagccgcat atatttctta
    24901 tgcatttacc gagattgctg caatatatcc aataacaccc agttcgacca tggctgaatt
    24961 agtagatcaa tggtcagcag caggaaaaga aaatgtattc ggtcaaccag taaaagtagt
    25021 agaaatgcaa tccgaagcag gagcagctgg tgtggtccat ggatcattga aaactggtgc
    25081 actgacaact acatataccg cttctcaagg gctgcttttg atgataccga atatgtacaa
    25141 gattgctggg gaactactgc caagtgtttt ccatgttgct agtcgggcag tgacgacaaa
    25201 tgcgttgaat attttcggag atcacggaga cgtgatggct gcaagacaaa caggatttgc
    25261 tatgttagct gaaagcagtg tacaagaagt gatggattta gcacctgttg cccatcttgc
    25321 ttctattgaa ggagaagtac cttttttgaa tttctttgat ggattccgta cgagtcacga
    25381 gattcaaaaa atcgaagttt tggaatatga agaattagct cctctgatca atcaggagaa
    25441 attagcttca tttcgtaaac gagggatgaa tcccaatcat ccgactgtta gtggtacgaa
    25501 ccagaatcca gacatccatt ttcaacaacg ggaaacgatc aacccctatt atgataaatt
    25561 acctgaaatc gttcaaaaat atatgaaaga gatcaaccga ttacgaggaa cttcttatga
    25621 cttggtgact tattacggag cagacgatgc ggaagaagtc attgtttcta tgggatcagt
    25681 tgcacagacg attgaacaaa cgattgatta cttgaatgca aacggacgga aaacaggttt
    25741 tttgaatatc catctttatc gtccttttcc agtggaaaca ttcgtgcaaa agttgcctca
    25801 tacagtaaaa acaattgctg ttcttgatcg aagtaaagaa ccaggggcag gtggagaacc
    25861 tctcttatta gacgttcaaa gtgcgttata tgattctgaa cttcgcccgg ctgttattgg
    25921 tggtcgctac ggattggggt ctaaagatgt gactcccgac caaatctcag cagtttttga
    25981 tgaattgaaa aaagacccaa gcatacgcaa aaaacggttt accataggga tcgtggatga
    26041 tgtgacttat caatctttgg aaccaaagga aagtctggat ttgacagaac cgcaaacatt
    26101 ccaggccaag ttttggggat ttggatctga cggaacagtt ggagcaaata aatcagctat
    26161 caagattatt ggagatcaca ccgataaata tgcgcaaggc tatttttatt atgattcgaa
    26221 aaaatctggc ggtctgactg tctctcatct tcgttttgga gatacgccga tacgttcagc
    26281 gtatcttgtc gagcatgcgg accttgttgc ctgtcacaca ccagcttacc tgcatagcta
    26341 tgacttggtc aaaggattga agccaggtgg cattttttta ttgaatactc tttggagcga
    26401 cgaacaatta gaaacgcatc tgcccttaaa attaaaacgt tacttggctg aaaataacat
    26461 tagattctat acgatcaatg ccatgagatt ggcccaagaa gtcggtttgg gacgccggat
    26521 caatacggca atggaaacgg ctttttttaa attagctgac atcattcctt ttgatgaggt
    26581 gcttccgctg ctaaaagaag aagccctgaa aagctatgga cataaatcaa tgactatcgt
    26641 agaaaaaaac atacaagcaa tcgatcggac gaccgaactg cttcatcaag tcgcgatccc
    26701 agaccattgg agaacgttgg agatgcctga aaaaatcaat aaaaggaaca ctggttatat
    26761 acaagaaata cttgaaccag tgaatgcaca ggaaggaaat caactctctg ttggtacact
    26821 tgtagcaaat ggaatgacaa ctggcgaaat gcctttagga acggcttcta tggaaaagcg
    26881 gggcgtcgct ttagaagtac ctgagtggat tagtgatcgc tgtacgatgt gtaatgaatg
    26941 tgcctttgtt tgtcctcatg ctgccatccg tccattttta gcagatgaag aggaaatgga
    27001 agaagcgcca gaaggattta tcgtgagaga aatgcgaggc gcagacggcg taaaataccg
    27061 tatccaagtt tcagtggaag actgtacagg ttgtggtctg tgtgtagagg cttgtcccgc
    27121 aaaagaaaaa gctttagtga tgaagcctta tgaagaacag aaagaacaag ctgtgaattg
    27181 ggcttttgca atgacattaa gacaaaaagc aaatccagtc aaaggaaaga atactgtctt
    27241 gtctacacaa ttcaatcaac cgcttcttga attctcgggc gcttgctcag gctgtggcga
    27301 aacaccttat gtaaaattat tgacacaaat gttcggtgat cgaatgatga tcgcgaatgc
    27361 gacaggctgt tcttctattt ggggcggtgc ttctcctgct tctccttata cgaccaatga
    27421 atgtggtcaa gggcctgcat ggtcaaattc tttattggaa gataatgctg aatttggtta
    27481 tggtatgctt ctagcggcac agactcgtcg cgaggctttg gcaagttcaa tcaaagaagt
    27541 attgccaaaa gtatcagaag aactacgaga attgatggaa gactggttgg tacacatgtt
    27601 tgagagcgaa ggaacgcagc aaagagcagc aaaactgcaa gctgcgatcg aaagcgaacg
    27661 atcaattgtc cctgaactgg aggatatata caagagaaag gatctattcg taaaaaacag
    27721 ccaatggatg attggtggag acggatgggc ttacgacatt ggttttggcg ggatcgatca
    27781 tgtacttgcc agcggagcag acgtgaatat tcttgttttg gataacgaag tctattccaa
    27841 tactggcggt caaacctcaa aagcaacacc agcttcggca atcgctaaat tttcggctag
    27901 tggaaagtat gcttcaaaga aagatctagg aatgatggcg atgacatatg gaaatgttta
    27961 tgtggcgcaa attgcttcag gtgccaatca aatgcagacg ataaaggctt ttgaagaagc
    28021 agaaaagtat cctggacctt ctttaatcat tgcctatacg ccttgtatca cacatggttt
    28081 gcttggtgga atgaaagaat cgcttaaaga agcaaaagaa gctgtttctt ctggctattg
    28141 gtcattatat cgatataacc ctgcaatggt cgaaaaaaac aagaacccaa tgactttgga
    28201 ctacaaaaaa cctgatttct ctgctatgcc tgatttcatg aggaaacaag ttcgcttttc
    28261 ctcgctagag aacgcaaatc caaatcttgc aaaagcttta tttgaaaaga ctatccaaga
    28321 cgctaaaaca cgtttttaca actatgctgc actgacggga gaattagaaa aaatcaagaa
    28381 acgacttgaa ccagaggaat ctatcgatgt acccgttaag actagaaaag aaaaaacgac
    28441 ggaccttgaa gcagaagaaa gaagagctgc cagagcagaa cgacgtaaaa aaatggaaca
    28501 agctacaaaa gaaaaaaagg aaaaataaca gaaaaaatat aatgaataat tctctgaagt
    28561 ttaaaaatat ttattgcaga gaaaaataaa acgcatactt gcaattttcc tgaaaattat
    28621 gcgtttttct caagaaaaat acgagcagat cagcctctaa aattaaaaca tatgatatac
    28681 tagagaggaa atctcaaaag agaagagagg ctttttatgt catttcaaat cggggaactt
    28741 tttaatttgg actattggca gcaaatgttt tccggtgact tgttctctct gaattttttg
    28801 gtgaacatac ttgatattct ggttgtctgg tatcttgtat acaaactgat ccaactgctg
    28861 cgaggcacaa aagcgattca gcttctgaaa ggtgtagcta tttttattgt tatccgaatc
    28921 gtagcggaaa tcattggtct gcacaccttg tcttggctga tgaaccaagt cattacgtac
    28981 ggggtgatag ctgctatcgt gatttttcag ccggaaatcc ggagaggact ggaacatctt
    29041 ggacgcagtt cgcttttcaa acaatcaaaa agcgaacagc aggaagatga gaagatgatc
    29101 ttgtcttttg acaaagccat ccaatatatg tccaagcgaa aaatcggtgc gctaattact
    29161 atcgaacggc atactggatt agatgagtat attgagacag gaattgcatt agatgcagat
    29221 atcactggag agctgctgat caatatcttt attcctaata caccgctgca tgacggtgca
    29281 gttattgtaa aagaaggaaa gatcgcagta gcaagcgcct atcttcctct atcagaaagt
    29341 atgttgattc caaaagaatt cgggactcga caccgggcag ctgtcggtat aagcgaagtc
    29401 agcgacgcga ttaccatcgt tgtctctgaa gaaactgggg atgtcagcat tacattagat
    29461 aatgaactga tggctggctt atcccagcaa gaatatttgg caattttgcg tagagaactg
    29521 attgctgaag agaaaacaaa aaacaagaaa aacgtgattc aatctttcct agacggtatg
    29581 actaagggag gggatcgcaa atgatctcaa aagagaaacg gacaagcatt ctttacagtt
    29641 ttattgcatt gctgttcagt attgcgctgt acttcaatgc aaatggccag tccgttccaa
    29701 atacgctttc tggaaatgaa gcgtataatc aaacagttac tggggtgcca atcaagattt
    29761 cttatgattc aaagcgctac tatatccatg gatatgaaaa tgcagtgact gtcaaacttt
    29821 ccagtgcaaa ccgagttcag ttgaatgcag aggcaaacga agatacacgg atgttccgcg
    29881 tcacagcaga tatcactaag ctgggcgaag gaacacatga ggttccgcta aaaattcaaa
    29941 atttgagcag tgctgtcaca gcaaagatcg aaccatcgac tattacagta acggttgaga
    30001 aaaaggtaat aaaagatttc gatgtggaaa cgttgattcc ttcaacgttc acaccatctg
    30061 gatatgaact tgatgatact tccgttagtc ctaaaaaagt ctctatcact acaggtgaca
    30121 aaacattggc agaaatcaaa cgagtagtag ctgatgtgga cgcttcgatg gtcacagatg
    30181 atgggattaa tagtgaggtc ccaatacagg cgctgaatgc agcaggggaa gtattaagta
    30241 tcgtctctga tccagtgcaa gtaacagtga gagctgattc gatcaagcca agcaagtcgg
    30301 tacgacttta tgggatacag caaggaacac cagctgccgg ggtagaaagc tatgatttca
    30361 gtttttcaaa actggaagca gaagtaagcg gctctagcga tttgcttgca tcgatcggtg
    30421 acagtattgc tgtaccgatc aatgtctcag gtatcaatca tcgaacaact cgtaaaattg
    30481 aaataccagt agaagagggg ttaagtatca agcccgaatc agttagtgtg gagattacac
    30541 ccgtgttgca aacgtcaagc agtagctctg cttcgacgag cagtcggaca caagaaaata
    30601 caagtacgag tagaagtatt tcatctgctg attcaggaac ggaaccatcg acaacaacca
    30661 tttcttccag ttcggaatct gggacaagca gctcgaccac aagtacagaa gaaacatcag
    30721 aaacaagcga atcaacacaa atgagcagaa caaattcctc agaaaattaa gttaaaggag
    30781 taattttaca actatgggta aatattttgg aacagacggc gtacgcggtg aagccaacaa
    30841 ggaacttacg ccagaacttg catttaagct gggacggtgc ggaggttatg tcctaagcca
    30901 gcatgaagaa gaaggaaaac gcccacgcgt gttagtaggg cgggatacaa gaatctcagg
    30961 tcaaatgtta gaagaagcgc tgatttcagg acttttatcg gtgggtatcg aagtgttcca
    31021 gctaggtgtg atctctacac caggtgttgc ttatctgaca cgtttgcaaa aagcaagcgc
    31081 aggggtcatg atttctgctt ctcataatcc agcacaagat aatggaatca aatttttcgg
    31141 tgcagatggt tttaaactag tagatgacca agagctagaa attgaagcat tattagatgc
    31201 tgaagtggat gaattgcctc gtccatcagc tgaaggattg ggaactgtag aagaatttcc
    31261 agaaggctta ttgaaatatt ctcaattcct tgtacaaacg atcaacggtg acctttccgg
    31321 tttgaccatt tgtgtcgatg cagcaaacgg agcaacttca acaagtgtta atcgtttgtt
    31381 tgccgatctt gaaacagatt tttataccat gggcacaaat ccagacgggt taaatatcaa
    31441 tgatggcgta ggctctaccc atccagaacg tttagctgag atggtcgttg aaaaaggagc
    31501 agatgctgga ctagctttcg atggcgatgg cgatcgtatt attgcggtgg atgaacttgg
    31561 aaacattgtc gacggtgata aaatcatgtt tatctgtgct aaatacttgg cagaaaaaaa
    31621 tcgattgaaa aaagatacga tcgttacgac tgtcatgagt aacttaggat tccacaaagc
    31681 ggtggaagga atcggtttga aagatgtcgt tacacaagtt ggtgatcgtt acgttgtaga
    31741 agaaatgcgc aaaaacgatt ataactttgg cggcgagcag tctgggcaca tgattttctt
    31801 agattttaac acaactggag atgggatgct ttcaggaatt cagttactga atatcatgaa
    31861 acagacaggt aagaaattat cagagcttgc agcagaggta acaatctatc cgcaaaaatt
    31921 agtgaatatc cgagtaagtg ataaaaacgg tgcgatggat gttccggcaa tcaaaaaagt
    31981 gattgaagag gcagaagctg aaatgggatc agaaggaaga atcctagttc gtccttccgg
    32041 tacagaacca ttacttcgag tgatggcaga agcgcctact gatgagaaag ttaattacta
    32101 tgtcgataaa atcgcggcag tagtcaaaga agaaatcgga ctggattaag agatattttc
    32161 ataaaaaata aaagaacaag gaccagtgtc agatgtctga cactggtcct tgttctttta
    32221 ttattctact gtcactgatt tcgctaaatt acgtggtttg tcaacgtcga atccccgtaa
    32281 tagcgttgcg taatatgcaa gtaattgagt cggtacgact gtggttagag tagttagata
    32341 agggtggaca gttggtagca ccagctggtc actttccttt tctaatccct ctgagacaat
    32401 aactagtgtt ttagcgccac ggctttcgac ttctttcagg tttcctcgag tatgcgcacc
    32461 agtcacttct tcactgatga ttccaatcac tggtgttcct tcttcgatca aagcaatcgt
    32521 tccatgtttc aattcgccag cggcaaaacc ttctgcctgg atataactga tttctttcaa
    32581 tttcaaagca gcttcaagag aaacgttgta gtcagcgcca cgtccaatgt aaaaggcatt
    32641 tcttgtatcg ctgagatata cttctgccaa ctcttctaaa taatcttttt gggcgatgac
    32701 cgattccatc gcattagcaa ctaaactcaa ttcatgagtg atatcgaata caagagaagt
    32761 ttcaaattct tttttatcac cgatagcttt ggctaataaa gttagtacag ctatttgtgc
    32821 agtataagct ttcgttgatg ctactgcgat ttcgggtcca gcatgcaata gcagtgtaaa
    32881 ggatgcttcc cgcgaaagag tagaaccagc aacatttgta atcgtaagtg aagggtagcc
    32941 gagacggttg atttttacca gaacttggcg gctatctgct gtttcgccac tttgactcaa
    33001 gaaaataaag aatggttttt ttgaaagaag aggtgtgtta tagccaaatt cactggctaa
    33061 atgaacctca acagggatct gtgtcaattt ttccagagtc tgtttacctg ctaaccccgc
    33121 atggtaacta gttccgcaag caacgatata gattcgatcg ctatcagaca atgtatcgac
    33181 cagcttttga tcaagagaga cacgattgtc ttctgttacg tatttttgag cgatacgacg
    33241 catgataata ggctgttcgt cgatttcttt cagcatataa aaaggatagg tacctttctc
    33301 gatatctgaa gaatctactt gtgcttcgaa tgaagaacgt tctacgatgt cacctgcaaa
    33361 attttgaata ataacttttt ctgatgtgac ggtaaccatc tcttcatctt caatctcaac
    33421 gaattccttc gtatgagcaa gcatagccat tgcatcgctg gcaatcacat tgaatccatc
    33481 gccgagtccg attaatagtg gacttttatt tttagcgacg taaataacat caggtgctgt
    33541 ccgatcaatc aaagcgaaag cgtaagaacc tttgatgacc cgcaaagctt ttaaaaacgc
    33601 ctcttgcgtt gtagtatcct tcgcgaaagt ttcaattaga tgagcgataa tctcagtatc
    33661 ggtctcacca ataaagtggt catttgctaa ataagcaatt ttaagttctt caaagttttc
    33721 gattacgcca ttgtgaacga gcacgaagcg tccactttgg gaagtatgtg gatgggcgtt
    33781 ttcttcagaa ggttgcccgt gagttgccca gcgtgtatga ccaataccta tatttcctgt
    33841 agtatcaata tttattttgt ttttcaaatt ttgaattctg ccttgtgctt tgaccagata
    33901 atcagttgtg ccatctgaga caaagatacc agctgaatca taaccacggt attctaattt
    33961 ttctagaccg tcaatcaatc ctggtgttac attttttaat ccaatcattc caacaattcc
    34021 gcacataaaa aacatcctac tttctataat gaattcgtat gaatgcttcc agctgcagga
    34081 aaatcctttt gttttatctt tgcagatatt ttttgtggat tttctgtaga gtgcagtccc
    34141 tagggccatc cgccgaatct ttcgataaac cctctcctcg tcatctgttt acacagacct
    34201 ggcgctatcc taaaaggtat aactatcctc ctcttttgtt ttttatctgg aaacatttaa
    34261 aatcataacc ttttgcgaat gttcttgtca acaaaaaaag aataaaaaat aaattggtat
    34321 atatcaaatg ctttgtcaaa tgctcttttt atgtcaaaaa ataatgggtt tttatctatc
    34381 atctttttca ggagttcggt acaatagaaa gtaatcggat agaggaggag taaaaatgga
    34441 ttcaaaaatt gagatcatga cactgggaat gttaaaaaaa caattgtcca aattcgaagc
    34501 atcagctggt gtatcagatg atacgaaaat atttttagat actggctggg atagcatcca
    34561 agaaatcgca cctgatgcct tggaagttgc tgaagctcgt gagtttacgg tagaagatga
    34621 attgacaaaa gagtcgtttt ccgggtatgc gcgggaagaa aaagcagagc ggttcgacac
    34681 ttctgagcag tcagaaactg tgatcgtcat aaaaaacttg tattagaaag gagaaagaat
    34741 gaataagaga cgttgggcag cagttctgat tgctgcagga ttatttgtgg tttcgttagt
    34801 ttcagctagt ttgactaagc ctcaggaaaa cgatcaaatt gagggattga acagttggtt
    34861 atatggggat gaagaactag cttcaaatgt gttggaagag ggagacagta cgcaaaaaat
    34921 cgtaaaatta tcagtagatg gaacgattgc tgcgggaggg tcttcaggtt tatttgcttc
    34981 tgaaggttac aatcataaaa attttatgga acaactgaag gcgattgaag aagatccaac
    35041 agtcaaaggt atctttttag aagtaaattc tcctggtggc ggtgtctacg aaagtgcgga
    35101 aatcgctaag aaattggata cgattcgtaa agaacatgat atcccaatgt atgtcagcat
    35161 gaaaaacatg gcagctagcg gtggctacta tatttctgct caagcagata aaatctttgc
    35221 aacagaagaa acagtgaccg gctcaattgg tgtgatcatg tcaggattga attattcagg
    35281 acttctagaa aaactgggtg tggaagatac aacagttaaa agcggagcgt tgaaagatat
    35341 gggttcagga acgcgaccag agacaaaaga agaagaagcg gtgcttcaag catatatcga
    35401 cagcgcatat agtcgttttg tagcaatcgt cagcgaggga cggaataaat ctgaagaaga
    35461 agtcaaaaaa attgctgatg gaaggattta tgatggtgtc caagcaaaag aagtaggttt
    35521 agtcgatgaa ttgggatttc ctgaagatgc tttaaaagct atgcgaaagg aacaacagct
    35581 tgaagatgcg gaactagtag aatattcttc cacgccatct gggtttgcta gtacatggtt
    35641 aggaagtaaa ctagcagaat tccagggatt gaagccttct gagactagtc agatcttatc
    35701 cgttcttgag aatctaggga cagcggaatc accaaaagca atgtattatt acggaggtga
    35761 gtaagcatgt cagagctttc aaatgaaaat aaggaacaag aaaaagataa gcaacagcag
    35821 cctgctgtag gacctgactc caacttttcc caaaaggtac tgagctcttt tgaaagagag
    35881 cctttgacgc ccgaagaaat caaaaagaaa caacgtaaat ggaaaaagta tacggacaca
    35941 agaaatgaac cgccaaaagc aaatgatttt cctaagtatt tctatgcagg tttttttatc
    36001 cgaatgtttg ctttcttggt cgatcttttg tgtatcggag ctattacgcg tttaacactt
    36061 ggtattgcca aaaatctagg ttggcttaat ttttcagatt cttatctgag tatctacgga
    36121 ttcacagcct tacttattta tctagcgtat tttattttat tgacgaaatt caatcatggt
    36181 caaacgattg ggaaaatgat ctttggtatt cgagtcatca gctttgatga agaagaactt
    36241 agctggtcaa ccgttttagt tagagaaggt gcttgccggt tcattctaaa atttcctttg
    36301 ctgatgttag ggtatcttcc agcaatcttt aatcagaaaa agcaacatct aggcgatttt
    36361 ttcactaata caagtgttgt tacgatcaat ctgatcaaag cattcaacca ggaagtcaac
    36421 agctgatgag caataaaaag cagtaacagt tgaaagcacc ttctaaaagt cagatctaaa
    36481 atctagcttt tagagggtgc ttcagttatt tttaattgtc atgctttttt tgttttgcta
    36541 ctattgaaca tgagctgaac atgagcaaga ggaggacact gcgtgaagcc gattgatttt
    36601 ccagaaaaat atgagaattt aatgcgagta gcacaacaag cactagcaaa ccagcaatat
    36661 cagcaggcaa aagaattatt tcaacgagct tacgagttga aagaaagctt tgaggctaat
    36721 agcctgcttg ttttttgtct ttacgagttg gacgagaaaa aagaagcatt gaagcaagca
    36781 ctcttgcatg aaaaacagta tcttcaaaat gaggagtttg ctgaatttta ttttgacttg
    36841 ttaatcagta cacaagattt tttatatgct agaaaattga tagcaagtac agatttttat
    36901 gaagctttcg agcagcgggt cattgaaaaa atccaatttg cagaagaatt atccggacaa
    36961 atggagaggc agaagatcaa ggcactccac aataaatcgg aggaacttcc ttcgttggaa
    37021 cctgcacgtc agcttgcgtc tattgatgag atcgaacagc ttccttatca tgagtttatc
    37081 caaacagcga gtaaacttat tgtatcgcca gaggtccata ttttagcaag ggcaaaatta
    37141 ctggagacac ttaggcaatt gaacgaatgc catcctgttt tctatttgac gattgaagaa
    37201 aagttagtca aagtcatacc aaaagatctg ccaaagccgc agcagcaggc aagttaccag
    37261 caattatgtg tattttcaga tcgttacggt aatgaagatg cgctgcttag ttccgtgtta
    37321 aatgaagaat tcacgttgca atctgcaatt gtttatcctg tctatgatac atacatcgaa
    37381 gacccgaagc gctggtttca actgactgta gaggcgtgta caggaaaaac gatgtatgaa
    37441 ggtacagaag atgaaaaaca agatttttta aaaaaaagag aaaaaatttt gcagcaaatg
    37501 atcttcttcc attagatttt atcagtgaaa aagataaaaa tgttatgaaa aacaaggatt
    37561 ctatccattc aatatgttta caatttgaaa gggttagtgt actatttaaa ggtatgcatg
    37621 aaacgttttt gatgtataaa atgaatttct aaactcggag ggaattaaat gactgcaaca
    37681 tttgaaaaaa aaggcacgaa tgatggtgtt ttaaaattta cgattgcgca agacgaaatc
    37741 caaaaaggat taacaactgc gtttaataaa gtgaaaaaaa gcttgaacgt acctggattc
    37801 cgtaaaggga aagtttctcg tcaagtattc aaccgtatgt acggtgaaga agctttatat
    37861 gaagatgcat tgaatgctgt attaccagaa gcttatgaag ctgctgtcaa agaagctggc
    37921 atcgatcctg tttctcaacc aaaaatcgat gttgaaagca tggaaaaagg tcaagactgg
    37981 gtaatcactg ctgaagtaac agtgaaacca gaagtaaaat taggacaata taaagatcta
    38041 gttgttgaaa aacaagaccg tgaagtaaca gatgctgacg tggatgcacg tatcaaacgt
    38101 gaacaagaag cacaagctga attagttatc aaagaagatg ctgcagctga aaatggcgat
    38161 acagtagtaa tcgacttcga aggctttgta gatggcgaag cgtttgatgg cggaaaaggc
    38221 gaaaattact cacttgaatt aggttcaggc tcattcattc caggttttga agatcaatta
    38281 gttggtcata aatcaggtga aagtctagat gtaaatgtaa ctttcccaga agattatcaa
    38341 gcagaagatc ttgctggaaa agaagcagtc ttcaaagtga caatccatga agtaaaagca
    38401 aaagaagtgc ctgaattaga tgacgaattc gcaaaagatg ttgatgacga agtatcaaca
    38461 ttagacgaat tgaaagaaaa atatcgcaaa gaattagtag aaacaaaaga aaaagcagcg
    38521 gacgatgcaa aagatgaagc agcgatccgt atggctgttg aaaacgcaga aatcgttgaa
    38581 ttgccacacg tgatggttca tgatgaagta catcgttcaa tggacgaatt cttgaacaac
    38641 atgcaacgtc aaggtatctc tcctgaaatg tactatcaat taactggttc tacagaagaa
    38701 gacttgcata aacaattcga aggtgaagca gaaatgcgca caagaacaaa tcttgtgatc
    38761 gaagcaattg cagctgctga aaatcttcaa gcaacagaag aagaaatcaa tgcagaaatc
    38821 caagaattat cagaaacata caacatgcca atcgaacaaa tcaaacgcgt gttgacagaa
    38881 gatatgctgg aacatgatgt gacaatgaaa aaagcaatcg aattgatcac aggtacagcg
    38941 acagaagcga aagaatagaa atcaagtaat aaatcatttc tgaagaagct acttctagaa
    39001 aattgtttat tcgaaataag ggactgtgac acgattttgt cacagttcct tattttttgt
    39061 taagagtaaa aaatgcaaag gtataggtca gttgatcctt tgttaaacac tttttctttt
    39121 tactaaacta tgttatgata gtaaaagact gtaaagtctt tgatagtttc ttgcatactt
    39181 tttgcaagtt aaggggtgaa gaccatgtat gacaatccaa gtagcaatga gaccgtacgc
    39241 tgttcttttt gcgggaaaac ccaagaagaa gtgaaaaaga tcgtagctgg tccaggggtc
    39301 tacatctgta atgaatgtat cgacctatgt aaagaaatca ttgatgaaga attttatgaa
    39361 gaagctgtac gcgaattcac tgaagtccct aaaccactag aaattcttga agtattgaat
    39421 aactacgtga ttggacaaga tcgcgcaaaa cgctcgttag ctgttgccgt atacaatcac
    39481 tataaacgtg tcaatcagca agcacaagaa aacacagatg atgttgaact gcaaaaaagc
    39541 aatatctgct tgatcggacc tacagggtct ggtaaaacat ttttagcaca aacattagca
    39601 aaaacattga atgtaccttt tgcaatcgca gatgcgacaa gtttgacaga agctggttat
    39661 gtgggggaag atgtagaaaa cattttgctt aaattgcttc aatctgctga ttacaatgtg
    39721 gaacgtgcag aaaaagggat catctatatt gatgagatcg ataaaattgc gcgtaaaagc
    39781 gaaaatgtat ctattactcg tgacgtttcc ggcgaaggtg tccaacaagc cttgctgaaa
    39841 attctagaag gaactgttgc aagcgtgcca cctcagggag gacgtaaaca tccacatcaa
    39901 gaatttattc agattgatac gacgaacata ttgttcatcg taggtggtgc atttgatggc
    39961 atcgaaacga ttgtgaagaa tcgtatgggc gaaaagacga ttggatttgg tacacaaaac
    40021 caaaaacttt ctgaagacga aagtgtcatg cagcatatca ttcctgaaga tttactaaaa
    40081 ttcggattga tccctgagtt tatcggacga ttacctgtta tggcagcttt agagaaattg
    40141 acaacagatg atctggtacg gatcttgact gagccgaaaa atgcgctagt caaacaatat
    40201 caaaaacttc tttctctaga taataccgaa cttgaattcg aacctgaagc gttgagagcc
    40261 attgcgaaaa aagcaatcga acgcaatacc ggtgctcgag gacttcgttc catcattgaa
    40321 gaaatcatga tggatgtgat gtttgatatc ccttcaaatg aaaacatcga aaaagtgatt
    40381 atcacaaaag aagcagctga attgtctggt gagccaacag taatctataa agattcagag
    40441 aaaaaagcgg gttgatgaaa agaggctgcc gtaaaagtca ttttggcttt tgcgacagcc
    40501 gcttgcttta ttcatcacct tgttaaggag agagaccatg aaagtccatc aagctgaaat
    40561 tatcataagt gcagtggcac caaaacaata tccagataca caattgccag aaatagctct
    40621 ggcaggacga tcaaatgtag ggaaatcttc ttttatcaac acattgattg atcgaaagaa
    40681 tttagcccgt acttccggta agccaggaaa gacccagaca ttgaattttt atttgatcga
    40741 gaacgcatta cattttgtcg atgttccggg atatgggtat gcaaaagtat ccaaaactga
    40801 gcgagcaaaa tggggaaaga tgatcgagac atatttaacg actagagacc agctacgtgc
    40861 ggtcgtttct ttagtagatt tgcgtcataa gccttctcaa gaagatatcc agatgtatca
    40921 atttttgaag tattatgata tccctgtaat tgttgttgcc actaaggcag ataagatccc
    40981 tcgtggtaaa tggaacaagc atgagagcga aatcaaaaaa gcgttggatt ttgatcgcaa
    41041 tgacacgttt attctctttt cttccgttac aaaacaggga aaagaagaag cgtgggcagc
    41101 aatcgaacaa gcaattgaat cataaaaaga ataaaaaaga cttaccagtc aaactctttc
    41161 ataaaagttt ggctggtaag tctttttttg agcttaactt acttttgttt cttgtctttt
    41221 ttcttttcat tttgtggctg tggttttaga aatcgatcga tctcttcttt tggtgcaact
    41281 gttttttccg gatcgacagc aaatgattcg aaaagtttat ctaaattatc tgtacgttca
    41341 aatttcaacc ccattgtaaa cacctctttg tttcttttta cttagtctat catgtaatcg
    41401 tccaaattgg tttctaagac ataaagtttt agtttttctt tatcgttggt tataattaaa
    41461 ttataaaaag attttaaaga ggtgagaaaa tgattggcga agataaatta ttgatgacta
    41521 tccagctcac agacttaaat gaagaagaaa aaaaacagga aataagcgat tgggctattt
    41581 tgacacgtct gttgatccag ccaacattta tccgctcttt cacacaacaa gcggatccgt
    41641 atgatcttcg aaagcttcaa gaaaaaataa tgctagcatc ggtaagagac gaaagttggt
    41701 tagttgtagg aaatgacgag aacgaatgta gttttcgctt ggaagataat caattattga
    41761 taaaaaatgt attgtctatc tctgttttca aagaaaagca atttttgatt cgagacttca
    41821 ttcagaaaaa aatgattgct catggggtgt tcgcctatat gcgagcttat agcgagttta
    41881 tctatcacaa tacaaaacag atctctcaac ggctattgtt tgagaaaaag gatgaaatcg
    41941 aacatttacc taaaatgaaa caacaaaacg gggaagtagt agtcgattgc aaccaatttc
    42001 caggatacga tcttttttac caaggtttat gtttcacttc gtgttgggaa atgtattata
    42061 gtcgctatta tcatcaaatc atccctaaac cgatttttct cgacatccaa caagtagaaa
    42121 aagtgaagga attagaaaac gaagtcatat gtatccagct ttatcgagac ccctttaact
    42181 ggcaaaagcc aaacaatcaa atgtttcaat cctattttag ggaccagctt gggtttgatc
    42241 atcttgcttg ggacaatggt gtcggtttat tgaaaccgcc attcgttgaa tacatttaca
    42301 ctgaccatac gatccaatct gtccagtatc agaatgcaca aatgcaacct gttcctaaaa
    42361 agaatgcttc attttttgta acaaaaagtt atgatattca acaaggagat tataaagaaa
    42421 ggcgtgttcg cggaacacta aatgctcagg cgtattttcc ttgggttgac gagaatcgct
    42481 cgcgcatgat gtgctataaa gttatcgatc cgactgttgc tttggataat ggaatcgaag
    42541 cttattgtta ttatattagg gaatatctag aagtagaggt aacagatgaa aaatatcaag
    42601 agtatctttt atccttaaga atctatgttc cttcagaatc cctgtcagaa ttaccgttaa
    42661 aggaaatcaa acatagactc tctgatgtta ccttcaaacg aataaagaaa aagcggcgaa
    42721 gtatccaatt cgatgtcaag aaaggtgagc agcatctgcg tgtgcaattt ttagattacc
    42781 gagaattaga acaattgatt actttacaaa aaatatgagg tgaagaaaat ggataaacaa
    42841 ctctattatc gaataatggt tccggcatca ttattatccg tctgtctctt ttttgttcct
    42901 aattttccta ttcgatcagt catcggtttt ttggtcatgt ttggcgggat gggactttat
    42961 tattatcttc ttcataagga aagaaaacct gcttctgttt atatgaaaaa aacaagaagc
    43021 gaaaataaaa aacgataaaa cggcttgatg atagacaaaa aaataaaaaa ccagtctttt
    43081 tgactggttt ttt
//
LOCUS       DAIGOK010000007        39759 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.007, whole genome shotgun sequence.
ACCESSION   DAIGOK010000007 DAIGOK010000000
VERSION     DAIGOK010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.007"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(<1..830)
                     /locus_tag="NOA01_000272"
     CDS             complement(<1..830)
                     /locus_tag="NOA01_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCI0631504.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     "
     gene            complement(1054..2994)
                     /gene="thrS"
                     /locus_tag="NOA01_000273"
     CDS             complement(1054..2994)
                     /gene="thrS"
                     /locus_tag="NOA01_000273"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCI0631505.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3331..3510)
                     /locus_tag="NOA01_000274"
     CDS             complement(3331..3510)
                     /locus_tag="NOA01_000274"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HCI0631506.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3557..5380)
                     /gene="cadA"
                     /locus_tag="NOA01_000275"
     CDS             complement(3557..5380)
                     /gene="cadA"
                     /locus_tag="NOA01_000275"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HCI0631507.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5658..7937
                     /locus_tag="NOA01_000276"
     CDS             5658..7937
                     /locus_tag="NOA01_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCI0631508.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8030..8677)
                     /locus_tag="NOA01_000277"
     CDS             complement(8030..8677)
                     /locus_tag="NOA01_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631509.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8856..9575)
                     /locus_tag="NOA01_000278"
     CDS             complement(8856..9575)
                     /locus_tag="NOA01_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCI0631510.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9706..10857)
                     /locus_tag="NOA01_000279"
     CDS             complement(9706..10857)
                     /locus_tag="NOA01_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HCI0631511.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11074..11814)
                     /locus_tag="NOA01_000280"
     CDS             complement(11074..11814)
                     /locus_tag="NOA01_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCI0631512.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11818..12141)
                     /gene="rsfS"
                     /locus_tag="NOA01_000281"
     CDS             complement(11818..12141)
                     /gene="rsfS"
                     /locus_tag="NOA01_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCI0631513.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12198..12791)
                     /gene="yqeK"
                     /locus_tag="NOA01_000282"
     CDS             complement(12198..12791)
                     /gene="yqeK"
                     /locus_tag="NOA01_000282"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HCI0631514.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12775..13425)
                     /locus_tag="NOA01_000283"
     CDS             complement(12775..13425)
                     /locus_tag="NOA01_000283"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCI0631515.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13435..13746)
                     /gene="yhbY"
                     /locus_tag="NOA01_000284"
     CDS             complement(13435..13746)
                     /gene="yhbY"
                     /locus_tag="NOA01_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCI0631516.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13762..14871)
                     /gene="yqeH"
                     /locus_tag="NOA01_000285"
     CDS             complement(13762..14871)
                     /gene="yqeH"
                     /locus_tag="NOA01_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HCI0631517.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14868..15395)
                     /locus_tag="NOA01_000286"
     CDS             complement(14868..15395)
                     /locus_tag="NOA01_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HCI0631518.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15575..16360)
                     /locus_tag="NOA01_000287"
     CDS             complement(15575..16360)
                     /locus_tag="NOA01_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCI0631519.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16353..17222)
                     /locus_tag="NOA01_000288"
     CDS             complement(16353..17222)
                     /locus_tag="NOA01_000288"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HCI0631520.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17223..18602)
                     /locus_tag="NOA01_000289"
     CDS             complement(17223..18602)
                     /locus_tag="NOA01_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCI0631521.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18610..19029)
                     /gene="fabZ"
                     /locus_tag="NOA01_000290"
     CDS             complement(18610..19029)
                     /gene="fabZ"
                     /locus_tag="NOA01_000290"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCI0631522.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVAPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19054..19530)
                     /gene="accB"
                     /locus_tag="NOA01_000291"
     CDS             complement(19054..19530)
                     /gene="accB"
                     /locus_tag="NOA01_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCI0631523.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19533..20768)
                     /gene="fabF"
                     /locus_tag="NOA01_000292"
     CDS             complement(19533..20768)
                     /gene="fabF"
                     /locus_tag="NOA01_000292"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCI0631524.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20787..21524)
                     /gene="fabG"
                     /locus_tag="NOA01_000293"
     CDS             complement(20787..21524)
                     /gene="fabG"
                     /locus_tag="NOA01_000293"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HCI0631525.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21536..22453)
                     /gene="fabD"
                     /locus_tag="NOA01_000294"
     CDS             complement(21536..22453)
                     /gene="fabD"
                     /locus_tag="NOA01_000294"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCI0631526.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22560..22784)
                     /locus_tag="NOA01_000295"
     CDS             complement(22560..22784)
                     /locus_tag="NOA01_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCI0631527.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22837..23799)
                     /locus_tag="NOA01_000296"
     CDS             complement(22837..23799)
                     /locus_tag="NOA01_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HCI0631528.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23809..24249)
                     /locus_tag="NOA01_000297"
     CDS             complement(23809..24249)
                     /locus_tag="NOA01_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCI0631529.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            24555..25520
                     /locus_tag="NOA01_000298"
     CDS             24555..25520
                     /locus_tag="NOA01_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCI0631530.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRAQLPDLQ
                     EMTFEQKKKELQGLFQGQNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKHFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(25577..25807)
                     /locus_tag="NOA01_000299"
     CDS             complement(25577..25807)
                     /locus_tag="NOA01_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HCI0631531.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(25844..26779)
                     /locus_tag="NOA01_000300"
     CDS             complement(25844..26779)
                     /locus_tag="NOA01_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCI0631532.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKNHKKRVFLEKINASRLCF"
     gene            complement(26868..28091)
                     /locus_tag="NOA01_000301"
     CDS             complement(26868..28091)
                     /locus_tag="NOA01_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCI0631533.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(28108..29154)
                     /locus_tag="NOA01_000302"
     CDS             complement(28108..29154)
                     /locus_tag="NOA01_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCI0631534.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYSIRVPIQAISNGIDLEKYRPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(29318..31045)
                     /gene="ptsP"
                     /locus_tag="NOA01_000303"
     CDS             complement(29318..31045)
                     /gene="ptsP"
                     /locus_tag="NOA01_000303"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCI0631535.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(31045..31311)
                     /locus_tag="NOA01_000304"
     CDS             complement(31045..31311)
                     /locus_tag="NOA01_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HCI0631536.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            31790..34024
                     /locus_tag="NOA01_000305"
     CDS             31790..34024
                     /locus_tag="NOA01_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCI0631537.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            34172..34492
                     /locus_tag="NOA01_000306"
     CDS             34172..34492
                     /locus_tag="NOA01_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HCI0631538.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(34697..34921)
                     /locus_tag="NOA01_000307"
     CDS             complement(34697..34921)
                     /locus_tag="NOA01_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HCI0631539.1"
                     /translation="METEWQIRERKKQKKEEMLRLCRLLDLKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(35119..36699)
                     /locus_tag="NOA01_000308"
     CDS             complement(35119..36699)
                     /locus_tag="NOA01_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCI0631540.1"
                     /translation="MNNPELKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(37100..38476)
                     /locus_tag="NOA01_000309"
     CDS             complement(37100..38476)
                     /locus_tag="NOA01_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCI0631541.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(38604..39722)
                     /locus_tag="NOA01_000310"
     CDS             complement(38604..39722)
                     /locus_tag="NOA01_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCI0631542.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
BASE COUNT        11282 a         8563 c         6875 g        13039 t
ORIGIN      
        1 tattcttgga ttgcttctcg tgtttttttc actttttcgc taagcttttc gtttttagcc
       61 atatagaaat aaaaaatgat atcgatcgta gcaaattggg ccaaaagtga attagttgcc
      121 gcacttcgat tcgtgatttc ttttggtcta gatgtttgta cgagtacatc ggctatcttg
      181 ctaagcgggt tatttccaaa ttgcgtcaac gcaatgattt cgatattcat gcttttcgcc
      241 agctcggcta atgcgactac atctgcactt ctacctgagt ttgaaacaag ccaaagcact
      301 ttctttttct cattgctgac aagttgcggg agcaagacgt ggatatcttt ttcaaaaatg
      361 attggcttac cgacacgaga ccatttttgc agaatatctt ctactactaa agaagaggcg
      421 ccaaccccat ataaaaaaac agtctctgcc gattccaaac actctacgac tttttggatc
      481 gtttcttctt ctagaatatc tactgtttct tttatcgcta cttgtgcatt gttaccaagt
      541 ttgtttttga tagagtaaaa ggactcattt gcttctatat cactaaaacc ttcatcgatt
      601 ccagtctgta gttctgttga aatcgtgatt ttgaagtccg tcagactttg aaaaccgatt
      661 tttttggaaa aacgtacaac tgtcggcgca ctcaacccgg aagcttctgc taattcatta
      721 gctgtcattg tagggacctt ctcaatatta ccgataatgt agttggccaa ctttttttct
      781 gctgaagaat aatcttccat catactttca atacgactaa ttacgctcat aagttcctcc
      841 acccttttaa tgatagtcta tgtaagtata ccatatcaga cccttattca cggtcgtata
      901 ctggataaaa aacaaaaaaa caatccgccc aacagaatat cttatcccat caatcccaca
      961 gattctattg agcatctgtg tctttgagga atacctgtta tctattgttc ggattgcttt
     1021 ctatctacta cttttttaca ggaaatttac ctattagttt tctctgctgt aatttttgac
     1081 ttctgcttcc atagcatcaa taaagatatt caaatcttct acagctgttt ctttacttcc
     1141 ataacgacgt acgttcactg ttgcatcttc caattccttg tctccaacaa ctaattggta
     1201 agggatcttt tgtgtttgag aggcacgtat cttatagccc attttttcat tacgatcatc
     1261 aacttctaca cgcaacccct tcatttgcaa acgttctttg atttcataag cataatcgcc
     1321 atgtgcatca acagaaacag ggatgattgt tgcttgtata ggtgccaacc atgttggaaa
     1381 agcgcctttg tatacttctg ttaaataagc gacaaaacgt tccattgtag aaacaattcc
     1441 gcggtggata actactggac gatgcgtatt ttccccgtct tctccaacat atgtcaaatc
     1501 aaagcgctct ggcaataaga agtctaattg tatagttgat aatgtttctt ctgtaccaag
     1561 agctgttttg acttgaacat ctagttttgg tccgtaaaag gctgcctcgc cttctgcttc
     1621 aaagtaatct agttctaatt catccatagc ggctttcagc attgcttggg ctttttccca
     1681 cattgcgtca tcatcaaaat atttatctgt attgtttgga tcgcgatagc tcaagcggaa
     1741 acgataatct gtaatattga agtcagcata tactgccacc atcaattcaa gcgtacgttt
     1801 aaattcatct ttgatttgat caggacgaac aaatgtatga ccatcattca acgtcatttc
     1861 tcgtacacgt tgtaaacctg acaaagcacc tgatttttca tagcgatgca tcattcctaa
     1921 ttcagcgata cgaattggta attcacgata agaatggatc gtgtttttgt agaccatcat
     1981 gtgatgaggg cagttcattg ggcgaagaac aagcatttcg ccatctccca tatccattgg
     2041 cgggaacatg tcttcttgat aatgatccca gtgtccagat gttttgtaca actcaacatc
     2101 tcccattatt ggtgtatata catgttggta acctagacta acttctttgt ctacgatata
     2161 acgttcgatc gtgcgtcgga ttgttgcccc ttttggaagc cagaatggca aaccagaacc
     2221 tacttctggg gaaaccatga acaagtcaag ttcttttcct agtttacgat ggtctctttc
     2281 ttttgcttct tcacggagac ggatatattc tttcaaagct ttcttgtcaa aaaaggctgt
     2341 cccgtataca cgttgcatca tttgattgtt tgagtttcca cgccaataag cacctgccac
     2401 tgataataat ttaaatactt gtatacggcc agtggatgga acatgtggtc cacgacacaa
     2461 atcgatgaat tcgccttgtt gataagcagt aatcacttct tcttctggca attcactaat
     2521 cagttctact ttgtacggat cacttgcaaa aatttctaat gcttcctgtt ttgaaagaac
     2581 acggcgttcg atcggcagat tttctttgac gattttcatc atttctgctt cgattgccgg
     2641 caaatcttct gcagttactt ggttttgtcc attatctgta tcgtagtaaa accctgaatc
     2701 gatagctggt ccaacgccaa agtggatatt tggatagagg cgacgcatgg cttgggccat
     2761 caaatgagct gctgaatgac ggactaagcc aagtgcatct tcatggtctg gtgtgatgat
     2821 ctcaatactt gcatcttcct cgattggacg agtcaaatca acgagttttc cgttcacttt
     2881 accagctaaa gctttttttg ctaagctttt tgaaatgctt tcagcaattg ccaatgttgt
     2941 tgttccagac tcaaattctt tgactgcacc atctggaaaa gtaattttta tcataatagt
     3001 ttcctccttg aaatgttttg attgatcaga tcatcaagct gaaaaacaaa aaaatcctag
     3061 catctcttga aaagagatac taggacgaaa agttcgtggt tccacctaat gttcaaacgc
     3121 aaatgcgttc ctcaagcgtt gtaacggtcc gaccgccctt gcttcaacaa gggtttcgaa
     3181 agtggtcatc ctttggtttt ctcctggaaa agtttcagcc tagcttttcc tctcttatcg
     3241 aagactctca aattcagttg gctttctcat caatcaatta tgtgtttatt taaccactaa
     3301 taaatagaaa aatcaagtgg gaaactaaac ttattttttc cattccccat ttacaccgta
     3361 tgttcctttt ttcatctcat tgatgatgac atggatgtgt tcttttggtg cctgcgtgtt
     3421 tttatgaaca gcttccgtga tatctttcat catattttct aattgttccg gactgcgacc
     3481 ttctactaat tcgacatgta cgaatggcat aatgctctct cctctttctg atcgttcttt
     3541 tttcaactcg attctatcat actatgtttg tcttgggtac tgaatcagga aaaatggctg
     3601 gatctttttt tgctctcaag gcactcaaaa tagagaccgt atccacgact tcttgcaaaa
     3661 cagctccgaa taatgcgggg attagtcccg tacttgcaat taacatcaaa aaaatacaaa
     3721 taaagatgcc aatcagtaca gactgtttcg ctattctcat cgtatctctt gaaatcacta
     3781 ctgcttttgc tacacgcaag agatcatctt tcaagatgac cgcatctgca ctttcactag
     3841 cagctgtaga accatgagcc cccattgcaa taccgatatc tgcagcagca agtgctggtg
     3901 cgtcattcac gccgtctcct accatcgcaa caggtctttg atctttaggg atactttcta
     3961 aaatggtcag tttatcttga ggcaagctac gggcatgtac agaatctaat tggacttttt
     4021 ctgcaatcgt ttgagcaatg gcagatccat ccccggtgat catcatcaaa tttttcacat
     4081 caagttttct tagctccttg attgtctttg ctgcttctgg gcgaatctga tccgaaaata
     4141 ggatcgttcc tgcaaaagat cgatcaattg ttacatagat tgctgtattg gacgtttcta
     4201 tttcttcagc tgaaacaaaa gaggcacgtc caatctttac ttcatgatta ttgactactg
     4261 cttggattcc ttgaccggtc acttctttca gttgactaac aggatacagt tggctgtttt
     4321 tcacgttgtt cactaacgaa cgagctaaga tatggctaga ttcctgttct gcactagctg
     4381 catacagaag tagctcttct ttttttattc cattgactgg ctgaatcgcc tctacttcca
     4441 atatcccttt ggtaagcgtt cctgttttat caaaagcaat cgtttttgta cgtgcgagct
     4501 tttcaatcgt tgttccagtt tttacaacga tcccgttttt acttgaacgg ctcattcctg
     4561 caactaaagc aatgggcgca gctagaatca gtggacaagg tgatgccact accagtacct
     4621 cagcaaatcg taaagggtct tttgagaacc accaagaaat accaccgatc aaataagcaa
     4681 tcaacgtgaa agggactgca taacgatcag ctaaacggac aaatcgggca ggcttttctt
     4741 ttgattcttc gactagttta ataatcattt gatactggct gtctgcggca cgtttttcag
     4801 cgatcatttg gatcgatccg tctccattta aagaccctga cataatctga tctccaggtt
     4861 tcttggtttc cgggcgggac tcacctgtca aagaagattc atcgaccgaa gattctcctt
     4921 tggtcactcg accatctgcg ggtacaagtt cgccaggctt gactagtaac tggtcatcaa
     4981 tatttatttc atcgattact atatcctgca attttccttc tttgaatcga tgggcaattc
     5041 ttggggtgtt atctaacaaa gactgcagtt ctttacccgc tcgtttattc gcgtaatcct
     5101 ctagactatc tccgcccgta agcatgatca atatcatcaa actagcccag tattctttga
     5161 cggctaatgt tgcaaggatt gcggtaatcg ctaatacatc tacgccatat cttcctgaac
     5221 gtaacgtctt gatcatttcc aaaaacatca agaaggcgag aatccctcca gtaaatgcga
     5281 caatcgcaaa tgcaagggcc ggctggtgaa aaacaaattc acttaataaa gctatcccac
     5341 caataagcaa agtaagtaaa aattttttga aatgtgtcat tgttttttct cctcttttat
     5401 gtgacttaga gaggttagta ataaatttcc agctgcctct ttcatcttaa gcatagtcct
     5461 tgactagcac tttttaaaac aataggcctt aattgaaaac aactatcact atcagcgttt
     5521 ccaaaattca tcagttctac aaacaaaaat attcagcaag gattactttt tcgattcatc
     5581 agttaaagag tacctttttt cgaaaaacta tgctatactt ttaattcgtt atttacggcg
     5641 gaaggagcaa acatgcgatg aatgagaaac aactagaaca aacgcacgtg gatgaaacga
     5701 tcgaactaat ccagatcgaa caaaatatgc tggatacaaa acaacaagaa ttgactcagg
     5761 aaatgaatca ttcgacaaaa gacacagcta atcacaagat tcgagctggt tccaatgaag
     5821 ctttttatga atctgctgtt gaatatcgcc aacatgagca ggaattgctt ttgaaatacc
     5881 atactcttga agcacagcaa aaacgcctgc aaacgttgtc tgtcatgaaa gatagtcctt
     5941 attttgcccg tatcgacttt aaagaagacc gtgaaacaga aacattatat ctaggtatcg
     6001 cttctttaag agatctggca gaagaaacaa tcgttattga ctggcgcgca ccaattgcca
     6061 atctttatta tgaaggtgag attggtcccg cattttatga aactgaaaca gatagaatag
     6121 atgtcgatct gcagttgaaa cgacaattta aaatccaaga aggacgtatt ttatctatgg
     6181 tagatacgtc ggaagtaatc aatgatgact ttttgctaga gatattggat gaatcatcaa
     6241 gcaaccatat gaaaaacatc gtctctacga tacaaaaagc acaaaatgca atcattcggg
     6301 acacaacaag taaagtaatg ctgattgaag gaatcgccgg aagtggaaag acatccgctt
     6361 tgcttcaacg tattgctttt ctgttgtatc ataatcgcaa gtggctcgag gctgaaaacg
     6421 ttcttctatt ttcacccaac catttgtttt ctgattacat ctcgactgta ttgccttcat
     6481 taggtgaaag cggcgtgcct acacagacat tcaaaaatta tttggtgcag ctgctgccag
     6541 agttttcttt actagatgaa cagcaacaag aaagtggttt tttgctaggg gaaaaagatc
     6601 cgatacaagt gatgaaaagc ggattgacct tagtcgatca gattgatcgc tatatccaag
     6661 gcatcacaaa ctacggtccc ttgttccgtg atatgaaaat aaatggccgt acgatcctat
     6721 caaaggaatc gattcgccaa tggtataaag aaacaaatga acaattacct ttacatcata
     6781 gactttctct attacaaacg aagctcctga aaaaattagg cggtctgcaa aaagacgaaa
     6841 cacgtcagca atgggtcaaa gatctggcgg aagaacagct ccaagaacta tacgcaacag
     6901 atccaaatct tgaatataca gaacaaaagg agcgttcatt acgtaaaaaa ctggccaacc
     6961 aaatcgtacg taaaagattc cgtcgaatcc atcgagggat catgaattat caatttgtca
     7021 atataacgaa acaatatctc cactttctac aaactgtgcc actttctctt ctaacacgac
     7081 ataaaatcag cgagaccagg tggcaggaac atttgacaac tgttcgtaca cacttgcgtc
     7141 aaagagaatt gcgtcaagag gatgctgtac tattcttttt actgatgcgc cgaacatatc
     7201 cagtgacggt tacacacaaa gctcgctata tctttattga tgaaatgcag gatttcgcac
     7261 catcacaagt ggcattgctg caagccttgt tcccaaaagc aaatttgact ttctgtggag
     7321 atctaaatca aaatgtcttt ggaaatgaaa cgatcgttgg atcattggaa gaccttttcg
     7381 tggaccgtga agtcactcgt ttccaattaa cgacaagcta tcgctccaca aaagaaatca
     7441 ctgattttgc taatcatttt attgtgaaag aacatcaagt agaaacaaca gcaagaaaag
     7501 gagaccttcc tttgctcatc caaggaacaa cagatgatat caaattgaat tggttagcag
     7561 ataccattcg aacgactcag ggaaaagccc ggtattggcg tacagcaatc atagctaaaa
     7621 cacaagaaga atgcgaagct ctttatgctc aactgccaga cgagttgaaa caacaagtcc
     7681 agctgatttt aaatgagagt gactttatga agcgttccat tattttactt ccagcctttt
     7741 tagcaaaagg tctggagttt gaccgagttt tcttgtggga tgtagatgca gaacattttt
     7801 cttctcagca agacaaattg atcctttaca ccatgtgtac acgtgcgatg catgaattga
     7861 ccctttttac ctccaataat gaaagtcctt tgttaaacaa tatggatatt tcatcttatg
     7921 agaaaaaagt actgtaagta aaaagacaac tgctatcatt aatgttagca gttgtctttt
     7981 tatttacaat aataattgca gaaaaagtaa ttcaatcatt taaggtcatt tattctatat
     8041 ataaatccaa atctgtttca ggatatttta atttgacaag cgaaataatc attttcagtt
     8101 cttccatgtt ttctggatat tctgggtcaa ataccttgct atgacggcca atcccgccat
     8161 tgtcgatcaa tcggtaaatt cgattccatt caatgaattg gtcaattgat tggaaattgc
     8221 tgattggata gccgccacaa tccaaaatct ctgatacacg aaatgctagg tatgcactat
     8281 cgatcacttt attttttttc gtgtctatct tctcatttct ttcgataaga attttttcag
     8341 gacgatgttt caagctccac tctaatagta gaaagaaagc ctccatccga tactcttctt
     8401 caaataaccg aaaggctgaa tcagaacgac gaaaaagcag cggaatcaga taattttgtc
     8461 tataaatcag cattggtgcg cggtccacca tacactctaa ataacggttc agataccctg
     8521 taccgacata gtttcctaat cgatagcctt caacgccgaa tcttgcgtat ttttgcgcta
     8581 attttgaaca atcagttacg ttcacagcta actcttctac tttgcggtaa ccgcttaaga
     8641 tacgttcaaa aggttctaaa gtgatttttg cttccatatt cttccccgtc catcttccac
     8701 atacttcata tcatttcaca tgatacttat gctctcatca taccacaggt tgaattagaa
     8761 aagaaaataa atcacacaaa aaagcaagac aatctgttag tgagattgtc ttgcttttcg
     8821 aaaaagactg aatacttttc gttaaaactg gttgcttagt cttctaatgc cacattatgg
     8881 aacacatgct gtacatcttc tagatcttcc agtgcatcaa tcattttttc aaatgtttct
     8941 tgatcttctg gagatagtgt tacctcgttt tgagggatca tttggatctc agcgattgaa
     9001 aaatcagtga ttcctttttc ttttaatgct tcttgtatac tatggaaatc cgctacttca
     9061 ccgtaaacga tgatttgtcc ttcttcttcc attacatcgc gaacatcgat gtctttttcc
     9121 attaggtatt caaggatctc gtcagcgtct tctcctgcaa aagcaaagat tgctgtattg
     9181 tcaaacatgt aagaaaccgc tccggaaaca cccatgttcc cgccattttt cccaaatgct
     9241 gcccgtacgt ctgctgctgt tcgattcaca ttgttcgtca atgtatcaac aatcaccatt
     9301 gagccgcttg gtccaaatcc ttcatagcgc aattcagagt atgtttcatc tcctgcacct
     9361 tttgcttttt cgattgctcg gtcaataata tgttttggta cattataagt tttagcgcga
     9421 tcaatgacga aacgtagttt ttggtttgca tgcggatcag gatcacctga ttttgctgcc
     9481 gcgtagattt caatcccaaa ttttgcataa atccggctat tattagcatc tttagcagtt
     9541 ttctttgcta cgatatttgc ccatttacgt cccatatttt cacttcgctt tctgatttga
     9601 tagtttcata acgcaaaaac gtcctgttcc attatacttg tgaatcacga ttctgtgaag
     9661 ctttttttct ttcttccttc attcttctca tttttagtca attatctata ctcggattgg
     9721 gatttgcccg aaattctgtt ccggaatatt gcctcccaat tgatagattt gatcacttct
     9781 aactaacagt tccgcttctt gtgcctcttt ttttccgatt cgggaataaa tagaaatctc
     9841 ttttttttct tttaagaatc ttcttccagc atcggtaaat ccaagtagtc gaagataatt
     9901 tttattttgt tggacaggaa tctcttgctt tttgatattc agcaagacat aacaagataa
     9961 tcgttggatc cgtgtccatg tatatctttt tgtttttacc tttgacacaa aatcctcaaa
    10021 actatcagca gttgcagctg ctgttttcat gcgcgattct attccttcgg tcatctgata
    10081 gatatctgtc agttccagca aagtagaaga taacaaccga taacgcaaat acggccaata
    10141 agattcccaa gtctgcagcg aacaagttga aagatcttct tctgtctgag gcggcacaag
    10201 tccagctatt ctttttcctt cttctaacgc atggcgaatg gctgttgcac tggctatcct
    10261 ctgatctgtg atgatttttt catgatagcc tgcactttta cgtcggatcg gatgtaattc
    10321 catagggtgg acatacttgg cattttcctt tgcataactt aatcccaaga tatgatttgg
    10381 cgaagaaaaa tcaagcgtaa tatcgggata cagactgcga aaaacctcag tcattttttg
    10441 cggataacta tacgtttgat cagacagttc ttgaaatgcc tcttctatcc tatcttgatt
    10501 ctcttggaca aagcgtccga aactgctgta atcaaacgaa gtctcttctt ctgttccaaa
    10561 acacaaactg tcacagtcca gtgtttgcaa gatttttact gcacctttag caaaatagtc
    10621 agctgactga accgcccatt cgacgggaag ctccactact aaatccacac cattttgtaa
    10681 agcttcattc gcgcgatgcc atttatcgat gatagccggt tcgcctcttt gcaaaaaatt
    10741 cccgctcatt acagcaatga cgacctctgc tcctgttatt tttcgtgcca tttccacatg
    10801 atagcgatgt ccattgtgaa aaggattgta ctctacgata atcccgcagc ttttcatgcg
    10861 catcctcctt catagttatc gataaaaaag aaatagagac aagtaaaaaa aggcaaaaat
    10921 cggaaaaaat acccgaacta tataaactat tctcgcaaac acaatacttc tgcgacgaaa
    10981 agctgcactg caagagcaat gcctatatcg ttcggatata taatttatgt tgaagattta
    11041 ttcttttctt gttaactcga gcaggcaaaa ccgctagtct ttgtatgcta caaagaacca
    11101 gcgctttgat ttctctgatg ctgcttcgtc tgtaaaatca gaaaatgctt cgactttcga
    11161 aaagccgctg ttttccagca ttcttaaata actttccaat ggataggtac gctcttgatg
    11221 tagttcatct tcacgaataa atcgaccaga cccttcttgt tcttcaacga aaaaggtaag
    11281 aaaatgttcg atgctgtgtt tctcgcttcc tggataacta tcccacaaaa aagcaaaatt
    11341 atccccttga taatgatagc tatattctgg aaaaacctca tctacttgat aggtggagtg
    11401 tacgtcaaat aaaaatcggc cattttcttc taacgcttga tagacttcat caaacacttg
    11461 ctgtacctct tgtctatttt tcatatagca cagcgaatca gaaaaacagg taattgcctg
    11521 atattgtccg atttctgata aatcgagcat attgccttcc acaaattgga tcgatgcgtc
    11581 ttcttcatat gctcgctggc tggcaatcat caacatctct tctgaaaggt ctaaccctgt
    11641 cactttatat ccatcttttg caaaggcaac cgctaatgct ccagtaccgc atgccagttc
    11701 caaaatattt tttgtgtctt cttgtaaatg acgtttcgaa aaagctaacc attgctgata
    11761 aagtgtttcg tccatcactt catcgtaaat gaaagcaaat gtttcataag ccatatgtta
    11821 atccaaccat tcactaagat cgactagcgg agcatctgac catagttttt ccagattata
    11881 gaaatttcgt tctggcgcat ggaaaacatg tacgatcaca tctccaagat cgatcaatac
    11941 ccattttccg ccttcttttc cttcaatacg ttttacttca tatttgtttt cttcttcttt
    12001 atcaatgatt tcttctgtaa ttgcgttgat ttgacgttcg ctatttgctg aacagatcat
    12061 gaaataatct gccaacaagg aaacatttcg aacgtctaaa gcgataatgt cttctgcccg
    12121 ttttgaatct gctgcttcaa tcgcaatttt taattgttct ttactatcta tgatgatttc
    12181 ctcctaatat gagcagctta tttttttgct acccagtaat tatatgtttc gattgttttt
    12241 ggataaatct gttgttcttg cgcaagcaaa tgggccaatg tatgttttgt ttcaaatgct
    12301 acagcttcgt ctagatcagc ccatgcaatg gctcgtgcat cttgtacccc aggaaaatct
    12361 cgacctggtt caatatagtc tgctacataa atgattttgt caaggagact catcttagca
    12421 gccccagtcg tatgcacccg gattgcatga agaatctctg catcggtgat tcctaattcc
    12481 cgctctacaa acgaagcgcc aacaagacca tgccagattg cattattata attcaataac
    12541 tccggatcaa aaccgtctct tacgattacc attttgaact cctcatctgg tcgttctttt
    12601 gcataatcat gtgtcaatgc agcaatacta gctttttcag gagaagcacc gtattttttg
    12661 gcaagagcca cggctgtttc ttctactcca agcacatgct tgaagcgttg ttcgctcatt
    12721 ctcatctgta ttttttgcat caggttttct ctagaataag cagtatattc attactatag
    12781 tccatattca tacaaccctt cattttgaat ataatcaatt actttatctg gaacaaggta
    12841 gcggatagaa cagccttctt tgatcttttg acgtattttc gtggaactga tgtcgatctc
    12901 aggtacatct acccaaatta caggatatgg agtatccgtt gtgtatcctg gtcgacgtat
    12961 acccacaaag ttgaccatag aagtcagctc atcgatttta taccattttg gcaaatattc
    13021 taccatatcc ccaccgataa taaagtaata atctgtatcc ggattgtttt gcgtcaattc
    13081 cttcatagta tcataggtgt aacttttccc accacgagct aattcaatcg tttcaatctg
    13141 caaaaaagga ttgtcttcca ccgctaattc caacatgttg agtcgatgtt ttgcatcaat
    13201 cgtctgtttc tcatcaacat gaggtggctg ataagaaggc atcaaaaata cgcggtcaag
    13261 acctaaattc cggccagctt gttcagccat cactaaatgt gcaaggtgaa cgggattgaa
    13321 tgttccgccc aagatcccta cttgcttttt cttctctaag aattgaggca tttcttcagg
    13381 aaataattgt gaacgaacaa atgtcttagc ttgcgtattc atcatggtca ctccttatat
    13441 tgcttttact tcttttgata gcctttgata cttttctttt gtggaaggtt tgtataatac
    13501 aataacacgt ccaatgattt ggacgatttc gcaatctatt tctttttcta atacttgagc
    13561 tgcttcttct gcaatctcat ctgtattttg aagtaacgat actttgatca gttctctttt
    13621 ttctaaagct tcttcaattt gaacgatcat tgctgaattg atcccacctt ttccgatttg
    13681 aaaaatcggc tgaagatgat gcgctttgct acgtaaaaaa cgcttttgtt ttcctcttaa
    13741 actcataatt ttccttcttc cttaaatcaa tgctttgcgt ttcaatacat ctacaccctt
    13801 tggcgcccag cccgcaatta ctactggcgc tgttacagtg atccagccta gaccggcaaa
    13861 aacgatgtct gttttttctt tcacagaaaa ctcaaaacga acaagctccg gaaatgcctc
    13921 gacttcatca ggacgaggag gctgtaataa tccacctaca tgtttttgat aaaattcgtc
    13981 tgctttttcc agtttcgttc gatgaatatt tacatcattt gacacataag caataaatga
    14041 agcacgattg ccgctgataa aatcaaatct tgcaagtccg cccaagaaaa gtgtttgccc
    14101 ttcattcagc tgataaactt taggtttgat ttcttttaac ggtgcaatga tccgcaaatc
    14161 ttttttacct aagtagtgcg ccatttgatg gcgatggata attcccggtg tatcaatcaa
    14221 aaaatgcccg tcatctaaag gaatctcgat tttatccaag gtcgttcctg gaaactgcga
    14281 agttgtgatt acatcttgta ctcccgctgt atttttgatg atttgattga taagcgtcga
    14341 ttttccgaca tttgttactc cgacgatata gacatcccgt ccatcgcggt attcttcgat
    14401 tttttctaat agttccgtca tctcatgaga tttcttagca ctagttaata atacatctac
    14461 tggacgaatt cctgcttcgt aagcacgttc cttcatccat tgtgttaatt tagttttttt
    14521 caatgattta ggtaatatat ccactttgtt cccaactaaa agtactggat tgtcaccgac
    14581 aaaacggtgt agtcctggga ttagtgaacc attgaagtca aaaatatcca ccacattgac
    14641 gatcaatgca tctttttgcc ctaattcatt cagtaagcgc aaaaagtcgt catccgtcaa
    14701 tgagacatct tgggtctcat tataatggcg taatctgaaa caacgctgac aatagacttc
    14761 cccggaagct aaaccttttt ccaaagccgt ttgcggcgtg tatcctaatt caccgggttt
    14821 gtttgtttgt ataacggcac cacagccgat acattgtaat tcttctgtca ttcgattcca
    14881 cctcgccagc ccatatcagg gtgttttgct aatagatagg ccataattct tttttcccaa
    14941 aaacgattaa attgtgtctt ccaactatct gttgcgataa tgggttttac aaggatgcta
    15001 cgtatccctg cacgattggc tccacgtata tcagtcataa tctgatcgcc taccattact
    15061 atctcatcat ctgctaaatc caattgtttc tttgctatgt tgatccctct tgctaaaggt
    15121 ttcattgctc gtgcaacata caaaagatca aatttttcaa tcgctcgcgc gacacgtttt
    15181 gaattattgt ttgaaacaac aacaacaggg attcctgcat ttttcatttc taaaatccaa
    15241 gtaagcaact cttctgttcc atctggatta ttccatgcaa tcaacgtatt gtctaaatcg
    15301 gtcaatactg ctttgatgcc tagattcttt aattgggcag gtgtaatctg atagatggct
    15361 tctaccatcc atgttggttt gtaatttgaa aacatgctgt ctcctcatct gcaaaaaatg
    15421 aaggagcaca taaagtactc ctcaaccccg tattatactg catatcagca aaaaaaacta
    15481 cctctatact cgttcgtctt tcagaaaaag catttcttta caaaatgacc aaaaataaag
    15541 attgatcaca gaaaaattta gaaacaaaaa acaactaata ttggcggaac ctcttgtatc
    15601 gtttttccag taatttatgc tgatctaatc gaacaagttc cgtcaatctt tcaatcaacg
    15661 cttttttcat aatgtgtgcg actttatatc gtggcaactg ctcgccgttg aacgtttcag
    15721 gaataactcg atcaacgatt cctaactcgc gtagttctgg cgcagtaatt ttcatcaact
    15781 cagctgcttc tgcggcgcga ctaccgtctt tccacaaaat cgacgcaaat ccttctggag
    15841 aaagcacagc ataaatcgtt tcttccagca tccagacttc atccgctaaa cctagagcaa
    15901 gggcaccgcc acttccacct tcaccaatga tgatggcgat gatcggtacc tttaaatcag
    15961 acatttcgag cagatttctg gcaatagcct ctccttcgcc gcgctcttct gctcctacac
    16021 cgcaatatgc acctgctgta ttgatcaagg taatcaccgg tctattgaac ttttccgctt
    16081 gtttcatcag tcgtaaagct tttcgatagc cttcaggatg gggagaacca aaatttctgc
    16141 ggatattttc ctgcagatcc cgtccttttt gaataccgat cactgttacc ggtttgccat
    16201 ctaatcgagc gattccacca acaatcgctt cgtcatctgc aaataaacga tcaccatggc
    16261 attcctcgaa agaatcgaag atctcttcaa aaaaatccaa gcttgtccat cgttctttag
    16321 ctcttgctaa ctgaacgaca tcatgtgctg tcttatccac gatctatcca tcctttctgt
    16381 gtatgcaatt caagaagcgt atggattttc tgtttcaatt ccatgcgggg aacaatctga
    16441 tcgacaaaac catgagataa taagaattca gccttttgga aatcttctgg aagttcctgc
    16501 ttgatcgtct gttcgatgac gcgtcttcca gcaaaaccga tcaagctttg gggttctgct
    16561 agaataatat cgccttccat tgcaaaactg gcagttactc ctccggtcgt cgggtccgtt
    16621 aaaacagtta gataaaaaag accttcattg ctatgccgtt tgacagcagc agagattttt
    16681 gccatctgca ttagagaaaa gattccttct tgcatccgtg cgccacctga agctgtgaac
    16741 aagacgactg gaagactttc ctttgttgct cgttcgaata atctggtgat cttttctccg
    16801 acaatcgttc ccatacttcc catgataaaa ttaggatcca tcactccaat aatgaaggga
    16861 atcccctgaa tggttgcttt accagtgagg actgcttcat gaagtcctgt tttatcttgc
    16921 atttttttaa ttttttccgt atattcggga aatgctagcg gatctttggt tacaagatcc
    16981 gtatcccatt cttcaaaact tttttcatca ataatcaaag ctagacgctg ccatgcaccg
    17041 attcgaaaag cgtatccgca atgagggcat actttttctt caccgatgtc tttcgtataa
    17101 aggatatgtt tgcaatttgg acactttgcc cacatgttat caggaacaga tggcgagtta
    17161 gcaggtttgc tctctctatt aggattgatc cgaatatatt tctttttctt aaacagagcc
    17221 atctatgcgc ctcctatctc cgtttctggt gtccaattcg gtaaaaattc ttcttgtaaa
    17281 aatgctgtgc tgtaatcacc tgctatgact gcaggatgac taatcaaatc catctgaaat
    17341 tctgcgttcg tgatgattcc ttctgtgata aattcactta aggctcgctg catcttcatt
    17401 aatgcttcaa aacgcgtatt tccatggacg attatttttg caatcattga atcataatat
    17461 ggcgggatcg tatatcccgg gtaaactgca ctgtctactc gcagtcccat cccacctgct
    17521 ggaagcagta agttttggat cttccctgga gaaggagcaa aatggaaagc aggattttct
    17581 gcattaatcc ggcattcgat tgcgtgtcct tggaatacta cttcttcttg acacacattc
    17641 aaaggctcac cggctgctat ctccacctgt ttttttacta aatcgattcc tgtgaccatt
    17701 tctgttactg gatgttccac ttggattcga gtattcattt ccatgaaata aaaatcgcct
    17761 tcttcatcca tcagaaactc gatcgttccg gcattttcat aattgactgc tttagctgct
    17821 cgaacagcag cgtctcctag tgcttctctt tttgtttgtg agatcactac agaaggagat
    17881 tcttccagta ctttttgatt gttgcgttgt aaagaacaat ctcgctcgcc taaatgaatg
    17941 acatgaccaa atcgatcacc taagatctga acttctatat ggcgagcagg ataaatgatt
    18001 ttttccaaat acatatcatc atttccaaat gccgctgctg cttcttgctg agctgaagaa
    18061 aaatgctgtg ggagctcttc ttttgattga actttccgaa tgcctttccc gccaccacct
    18121 gcagctgctt tcagcatcac tggataacca attctgtctg ctatttccaa tgcttcttct
    18181 actgtagata atacaccagt acttcctgga atgactggga catttgcttc ttgcatcaat
    18241 tgacgcgcat tgatcttatt tcccattgca tcgatcgttg cacttttagg accaataaat
    18301 gtgatattgc actcttcaca catggaggca aatcggctat tttcagataa aaaaccaaat
    18361 cctggatgaa ttgcttcggc ttttgtcacg attgcagcgc tcagtacttc ctgaacattc
    18421 agatatgaat caacggcttt tgccgggcca atgcagattg cttcatctgc taactgtgta
    18481 tgaagtgctt cggcatctgc ttctgaatat atcgctacgg tttggatccc tagttcgcgg
    18541 catgcacgaa tgatacgtac agcgatttca ccgcggttag ctatcaatac tttagaaaac
    18601 atatttcacc tatccaatca taaaggttaa ttccgcttcg gctacttttt taccgtctac
    18661 tttagcgatt cctttgccta ttccggctga agctttcact ttcaatatct caacttcaag
    18721 gtataaggta tctcccggtg caactttctt acggaatttt gctttgtcca atccgccgaa
    18781 ataagccgtc tttcccttga attgttcaag agaaagtaat gcaactgctc ctgcttgagc
    18841 cattgcttca acaatcaata cccctggcat aacaggttct tctggaaaat gtccttgaaa
    18901 aaagggctca ttgatcgtta catttttctt agcaacgatc cgttctcctt caaccatttc
    18961 ttccactcga tcaatcaaaa gcattggata acgatgtgga atgatttctt ttatttcttg
    19021 tatgttcatt gttttccctc tttcctgctt tgcttatttt tgctgaatcc taaagagtgg
    19081 ctgtccatat tcgacaactg actcgttttc tacaaggatt tctgtgatca ctccgtcggc
    19141 ctcactagtt atctcattca tcagtttcat cgcttcaata atgcagataa cttctccttt
    19201 ggcaacttga tcacctactt ttttgaaagc tggtttgtcg ggcgctggtt gcagataaac
    19261 gattcccacg attggcgaaa caacctcgat tccatcaaat actgccgatt ctttttcttt
    19321 cattatttct ggagcagttg ttgcttgttc aggtatgaca ttgactggct ccgaaactgg
    19381 caacgtttcg tttgtttgaa ccatattcat gcctcgactt gttttatttt tattcatata
    19441 caattcaaat tgaccttcac gcaaatcgaa ttccgttagc gaggattgat caaattgact
    19501 gactaattct ctgatttcat tgatatccaa aattattcct cccagcgttt catgcacaga
    19561 accgcattat gtccgccaaa ccctaatgaa ttactcattg cataagtaaa acttgcaggt
    19621 ttgctttcgt ttttgacaac tgtcaagtca atctcaggat cttgtttttc tacattgatc
    19681 gtaggtggga taaattgatg ttctaatgcc agcaaagttg ccaaggcttc gatcccgccg
    19741 gctgctccca ataaatgtcc agtcatcgat ttcgtgctgc tgacgtaagt atctctgaaa
    19801 ttttcaccta atgcgatttg gattgctttt gcttctgcct ggtcattggc tggtgtactt
    19861 gttccgtgag cattgatata tccgacttgt tccggctgta caccagcttc ttccatcgca
    19921 agcttcattg ctttagatgc accagaacca tctggagtag gagctgtcat atggtaagcg
    19981 tcacagtttg caccgtatcc aacgatttct ccatagattt tggcaccgcg agcttgtgca
    20041 tgttctaaag attcaagaac tagaacgcct gctccttcac ccatcacaaa accattccga
    20101 ttttcgtcaa acggaatcga accacgatta ggatcagttg attctgagag tgctgttaaa
    20161 gctgcaaatc cagcaatccc catctcacaa atcgttgctt ctgttcctcc agcaagaatg
    20221 acatcagaat agccatgttt gatgttacgg aatgcttccc caattgaatt cgttccgcta
    20281 gcacaagctg ttacgatagc tgtacaaata ccttttgcac ctacacgcaa agcaatattt
    20341 ccagctgcca tgttggcaat cgccattggt acgaacattg gagaaactcg ttttgcacct
    20401 ttttcatgta gacgaataac ttggttttcg attgttggaa gaccaccgat cccagatccg
    20461 atgatgacgc caaaccggtc ttgattcatt ttttctttat ctaaaccgct catctccatt
    20521 gcttctaaag cagcgtagat tccatagatc gaaaacatat ccattcgttt gctatctttt
    20581 ttaataaaat atttatcgta agggaaatct ttgacttcac ccgctaaggt aatccctgtt
    20641 tctgaagcat caaatttagt gatcggtgcg atgccattcg tcccctcttt taagctattc
    20701 aaaaagctgt ctgcgtcatt tccaataggt gaagtgactc catagccagt aattaccact
    20761 cgattcattt gtttgctctc cttttcttat ccgtgcatga ccaatccgcc atcgacattg
    20821 atgacttgtc cggtgatata tggattttgt gctaagaaaa ctgctgtttg tgcaatatct
    20881 tctacttgtc cgaaacgctg catcggaatt tgcttttccg cttgttcctt gactttgtct
    20941 gctaaaactt ccgtcatatc agttgtgatg aatcctggcg caatggcatt acatgtaatg
    21001 cctctagttg cagcttctcg tgccacagat tttgttaatc ctactacgcc agctttacta
    21061 gctgcataat ttgcttgacc aatatttcca atcaacccag atacactaga gagattaatg
    21121 atggcccctt ctcgctgctt catcattttt ttcaatacgt gctgcgtcat attgaaggtt
    21181 ccaatcaaat tgatatccag acattttttg aaatcttctg catccattcg catcacaagt
    21241 ttgtcatttg tgattccggc attgtttact aacacatgga tcgatccaag cttttcttct
    21301 gcttctttga tcatttgtcc cgctttctca taatcagaga tgtcaccgga aactcctacg
    21361 cattttacac cgaatgcttc gatttcttct atcttttctt ttggaatctc tccgcgacca
    21421 ttcaaaatga tatttgctcc agcttttgca aaagctaagg ccatcgcttt gccgattcct
    21481 cttgtactac ctgtgataaa tacatttttt ccttttacat ccataacgtt cctccttata
    21541 gtattgctat tgtttcatca aatgtctgct tattctcaac acgcagaact ggtattgttt
    21601 tatcgatttt tttcataaat ccgcttaata cttttccggg gccgacttcg atgacttgtt
    21661 cgatgccgat tgtttttaac ttatggatac tctcatagaa acgaacaggt tgcatgactt
    21721 gctgttcaag tagtcccgcg attgtttctt tcttcattat ttccgttgtt gtgttgctta
    21781 tgataggaaa agaaggttca gagaattgaa tctgacttag gtccttcgcc agtttttttg
    21841 ctgccggttc taaaatagct gtatgaaaag gcccgctgac atttagaggg atcattcttt
    21901 tgaacccttt ttcttttaat agtaaaacag cttcgtctac tgctttttct tcgccaccaa
    21961 tcacgatctg ctgaggtgta ttataatttg caggagatac gatgccgtac ttgctagctt
    22021 catgacagct ttcctcgatc gtttcaatcg gcgcattcat cactgcaacc attttcccgc
    22081 ttccagctgg ggcagcttct gtcatataag cgcctctttt tgctactaac gcaactgctt
    22141 cagtgaagga tagcgcatta ctagctacta atgccgaata ttctcctaaa ctcagtccta
    22201 aagctgcatc tgggatgatt ccatgctcct tcaataagcg ataatacgcg atgctgactg
    22261 ttagaatagc tggctgtgta tattgcgtct gatccaaacg ttcattttct gtaaagcaaa
    22321 gttcagccat atcgtagccg agaatctcac ttgcttcatc aaatgtctgc tttacaacgg
    22381 cttcttcata taaatctttt cccatacctt gatattgtgc accttggcca ctaaataaga
    22441 atgctgtttt catgtttttc tccaatatat ccagaatata tgtttgattc ttcctattca
    22501 aaaaggccga gacattccta tttcaggaaa tgtcccagcc cccaacctaa ttttaatgat
    22561 tatgcagttt gtttttctac gtaagtcact aaatcttgaa ccgttttgat tccgtcttct
    22621 gtttcgattt tcacgtcaaa ttcatcttca atttcattga tgatttggaa taaatccaag
    22681 ctgtcagctt ctaagtcttc ttggatgtca gtagtcaatt tgatttcttc agtttctttt
    22741 cctaattctt ctgcgatgat ttcttgaatt ttttcgaata ccataataat atattcctcc
    22801 gatattgttt gctttttatt tttataattt aacagtctac aactccatga gaatacttcc
    22861 ccatgttaaa cctccgccat agcctgtaaa tattactttc tgctgttttc ctaaggtgat
    22921 ctttccacta gaaactgctt catccaataa gatagggatg cttgcagcag atgtattccc
    22981 atatttatcc atattggtca ggaacttttc ttgcggcatc tttacttttc gggcaatttt
    23041 ctcaataatt ctgaggtttg cctgatgcaa aagcagataa tcaactggtt tgtctttcat
    23101 gacttcttgg atattgtttg caacgtctcg tacggcaaaa tcaaaaatat ctctgccagt
    23161 catttgcaaa taatacgaac cttctgaatc ttctgaatag aacggatttt tattttgctg
    23221 gtagccagcg gtcaatgagc tacttctggt accgtcagct gctaattttt ctttcagcaa
    23281 ttttttctgt ggattcgctt ctaagagaat cccacctgct ccatcgccaa acaacacagc
    23341 agtcgtacga tcagaccaat cgattagctt agatagtgtt tcaccaccaa tcaccagtcc
    23401 cttagttgag ccgcagcgga tcattttttc agccatgctt aacgcataaa caaatcctga
    23461 gcacgcagca ctgatatcaa aagcgatagc atgagttgct cccaatttcc cttgtacttg
    23521 tgctgctaca gatggcgtcg tgtaatcggg cgacattgtt gctactaaaa taaaatccaa
    23581 atctttaggg tttagatttt tctgttttat caatttttcc gccactaaaa tgcataaatc
    23641 agaagtctcc tgatttgtta cacatcttcg ttcacgaata cccgtccttg acgaaatcca
    23701 ttcatcagag gtatccatca tcttggaaag atcattatta gtaataatct tttcaggaag
    23761 gacgctggct gttgcagtaa tcattccaaa ttgttccatt aaatatcctc acttatagtc
    23821 ttgcaagaag tcgtgcaagt ttttcagtgc atccagcaag gcatgctgtt cctccgtagc
    23881 cattccgtct aagatgttct tgaccatttc acgatgaaaa tgctgatgga cccgaaatag
    23941 taactttcct tttttcgtta gtcccaactt tacaacgcgg cggtcatctt cactgcggat
    24001 acgttcgaca tagccttttt ttactaactt gttaatagca gtggtcaagg tcccaacagt
    24061 aattgatagt tctttggcta cttctgaagt tgtttttttc ttgtacatcc cgattgcctc
    24121 gatcgtgtgc atctctgtga ttgacagatc tttgaattga gattttttca gctcagactc
    24181 ttcgattgtc aaaatatcat tgaagacact aacaagataa tcatttactg tttttaaatc
    24241 tggttccatg ttccaccgtc cactaattag tttgattatc aaatcttttg atgttcaaac
    24301 tatatcttaa cttttttcaa aatgcaacct taaaagatca tttttttact atatgagctt
    24361 tttttaagat tttactcatt taaaacaact gataaatcag catttataat ttcttttatt
    24421 agaaaaatca tctatctcac atattttgat tatcaaagta ttttctgtga acagaactat
    24481 agaattcctt aaaacttgtc cattatttca aaatttgtta tagtcttttt attcaaagcg
    24541 taaaaggaga tcctatgtta aaagaaaaaa tgaaaactct ccccatgctg ccgggtgtct
    24601 atcttatgaa agaccgtgat ggtaaggtca tctatgttgg taaagcaaaa cggctcaaaa
    24661 atagagtaag cagctatttt catcagaata aacagcattc aaaaaaagta ttacgtatga
    24721 ttcatcatat aacagatttt gatttcgtcg tagtagacac ggaattagat gctttattgc
    24781 tcgagtgtca attgatccaa cattaccgtc ctttttataa ccgacaaatg aattatttca
    24841 gtaattataa ttatgtacat attaccaata agggatttgt attaacagat actcctacgg
    24901 ctcgaacata cgggcctttt cgtctctata aaaaaatgcc gtctattttg cgtataatgg
    24961 aagaaactta tcaaatgcct tggttgtctg aaattagttt gttagctttg cgagcccagc
    25021 ttcctgacct acaggaaatg acttttgaac agaaaaaaaa ggaactccaa gggctgtttc
    25081 aaggccaaaa taaaaagcta ctaacttact tgaaaaaaag acaacaacat ttcatctatc
    25141 agttgaattt cgaaaaagcc ggtatgcttc aaaaagatat cgaattagtt acttatttta
    25201 ttagacggat ccaggaacaa aagcattttt taagaacacc atcgctcacc ttcagcatgc
    25261 cattagcagc agatgagagt cagaaaaagc actacctcgt ttgttatggt caacttgcag
    25321 aaacaatgat tacctctggt gacgtttctc ctgacttcta ttatgaaaag aaagaagctc
    25381 atttatcttt gaaacgtcaa ttgtcaaaag aagaaatcga tcctgtacaa atcttgatca
    25441 gttaccgcaa aaaattagaa aaagaacagt ttgaaattga actgttaaac aaaaaagaag
    25501 ctgaaaagca actaaattag ttgcttctcg gcttctttta ggcatcttca acttcaaatg
    25561 tcaattatcc taaaatttat tggattaagt catagatttc catcgctacg atatcgatat
    25621 tatcaaattc gtattgctga tgagtatctg tttcagttaa ttcaaatgta tcggtcgctt
    25681 tatcataagt tactgtacac ttctctacgc cttctttttc aaaacggcga acttgcagtt
    25741 cattatctgc tgactcgacc atagcctcca gtcgttttat gattgcgact aattgtgaat
    25801 gtgtcatcgg aggtcctcct tttcttattt ctgttatatt gtatcaaaag catagcctac
    25861 ttgcattgat tttctctaaa aaaactcgtt ttttatgatt tttttctggt atttttcctt
    25921 caggccacca aagtttgatc aaagcttggg ttccgtcatt ctcaaaacct ttatctgcca
    25981 actcttcata tagtttcaat gcttgggtag ttgctggcaa tacaagatcc attttttctg
    26041 cttcttctaa tgcaatcttt aaatctttga taaaatgttt gacaaaaaaa ccaggtgtgt
    26101 aatcttcttt taaaatacga ggagaataat tgcttaaaga ccagttagct gcacttccac
    26161 ctgaaagcgt ctcaatcact ttctttaaat caagtccgtt tttttgtgca tagacaagca
    26221 tttccgtcat tcctgtcatc gtacctgcaa tcatgatctg attagccatt tttgtatgct
    26281 gacctttccc agcagaacca tgcaatgtaa atgtcgtacc aaattctttg aataaaggca
    26341 gtacttgatc ataaacggct tcttctccac cgaccatgat cgtcagtgta ccattctttg
    26401 ctcctaagtc accaccggat accggtgcat ctaacgcatc tgcgcctttt tcttttgctg
    26461 tttttgcaat tttttctgcc aggcttggtg tacttgtagt caaatcaact aaaatatgtc
    26521 ctgagatatc tgcagaaaaa atccccgact gtccatagta gatttcttct acatctttgg
    26581 ggtaacctac cattgtaaag agaatattac tttgttctgc taccgctttt ggtgtatctg
    26641 cccagatagc tccttctgtt actaaaggat cagcttttgc ttttgtccga ttatagacag
    26701 ctacttcgtg tcctgcttct agtagatggc gtgcgacagc acttcccatg acgcctgttc
    26761 cgataaaacc aagtttcatt ttttcgacct cctaaaaatc aaaaatcctt gcttctatgc
    26821 tagtatacgc tagaaacaag gattttttca taaaaaatgt ttgaatttta tcttttaaat
    26881 gaatttaatt ttattttgat tcgttcaatc gagtcttctt cttcttttct tcgcatctct
    26941 tcttcatgat aaacaaccat gtcttgataa aattcgatca tagcttcgcc gaaatgtgta
    27001 gatgaaatat cataaagttt agcttctaaa acttttggat ctttaggaac ttcactggaa
    27061 atataatcaa taagtgctgg tgcaaagtct tcgtcggttt caaaagtttt ccctaatgaa
    27121 ggatgatcga acaatcggtt caaataagca tttccttcca cgacacaagg tgttccggct
    27181 gccatcgctt ctgtataagt aagcccttgt gtttctgaag tagaagcact gacaaaataa
    27241 tctgccgctt tataataaag tgctacatca ttgttgtcca cttctcctgt gaactgcaca
    27301 aattcttcta actgcagttc tgagatcaat tcctctaact cttctacata aggtcctttt
    27361 cctacgataa ctaatctggc gttaggataa gcggagatga cctgaggaaa cccattaatc
    27421 aaggcttgga tatttttctc ataagaaatc ctgcttaaag aaagaatcat caaatggttt
    27481 tcctgaagac ctagtgactc ccttaatttg ctgatatcct gctgagtgat atcagaacgt
    27541 ttgaattttt caatgtcgat accagtagga atcacgcgaa gcggtgctat tacaccatag
    27601 ctgcgtaatg tatcaataac acgttcactt ggacaaacta cacctgtcgt atgatttgca
    27661 aataatctag acaagtattt tacatgggat tgcctgatca cttttccttt accgatataa
    27721 tgaaggtagt cttcatacat cgtatggtaa gtatggatca gaggtatttt caacttctta
    27781 gctaccatct tcccaagtat ccctgctccg aattctgtat gggtatgaat aagatctagt
    27841 tctagttctt tggcaaccaa ataagcatac cacatgcctc taacaacaac tcttcgatcc
    27901 ttgaaagaaa taaatggtac acttggcatt cgaatcacat ctttttccaa cttatccgca
    27961 ttaggatcgg tcgttgtaaa aatatagact tcatgcccat gtctttctaa ttcttgtttt
    28021 aatgttttga tcgatgtagc aactccgctg acttgcggaa agtaggtatc ggtaaaaaag
    28081 ccgattttca tgtttttcgc ctccaaacta gtccttgacc tgatttactt tttttaattt
    28141 gctagagact ttttcattca gtacactttc gtatacattt ttaagttctt caccgatttt
    28201 attgatactt cgctcttttg ctgtttgata accggcttgt gtcgtactcg gtagttcttg
    28261 attggtaatc tgttggatca gtcggataaa ttcttcgttt gtcttcccca tatagcagtt
    28321 cttgctatct tctaaccagc cattatacac tggaatatct cgaacaagta cgttttgttt
    28381 gcttgctaaa gcttctaaaa caacgatccc ttctgtctct tcataagaag ggaaaaagaa
    28441 tagatctgca cctgaatacg caccttctat gatctctcca cgaatgtaac caggaaacag
    28501 gacattttca ggatgatctt cttttacaat tttccggatg ttccgaggaa tcgagtacat
    28561 gggaacatga ccaaaccaaa taaatgtgta ttcgggcatc ttcttagcaa tttctacgaa
    28621 atcaatgatt cctttacgtt cgaaaaacaa accgacacaa atgattactt tttgatccgg
    28681 agacagtttg aaatattctc tgaacttttg ttctttcatt ggatcagggc gatatttctc
    28741 taagtcgatc ccattggaaa tagcttgaat cggcacacgg atactatagc tttccaacaa
    28801 ctgttttgaa taaggtgttg gcgtgatcag atagtccgct ttttggtaca atcctaccag
    28861 atattttttt acgataggag aaagctggtt cgatccgata aaggaatttc ggaaatcttc
    28921 ctctgtagaa tgcgcatgat agacaacttt ttttccttct cttctggctt tattgaccat
    28981 attatgacta ttgattccgt atgtattgat gtgcaggatg tcataatctt ctttttcatc
    29041 tagtgtataa gagataccga cttccgtcag agcacgtttt tgatgatcaa gcgcacgccc
    29101 gattccagat tttgcaagca tcttttcact ttcaaaatat aataatacct tcaccttacg
    29161 tcccccttca cttatatagt ataacatagc ctacaaactg tcactcaaac ttaagcccta
    29221 tttttatcta ttcttactga caaaaacgtt ttcttaaaaa agaacaaaaa aactggcgta
    29281 gctcttttac aagccacacc agcttttgat acgttgttta ttttacatat tcatgaacaa
    29341 gttcaacgac ttcttccatc gtatcgcatt ctttcaatgc acgatcagca agttcagcca
    29401 ttttcgctgt gtccaagcgt ttcattaaac tacgcgtttt aagaatagat gtcgcactca
    29461 ttgagaactc atctagaccc atacctacta gcaatggaac agctgtttga tctccagcca
    29521 tttctccaca catacctgcc cattttcctt cagcgtgtgc tgcatcaatt acatttttga
    29581 tcaaacgtaa aattgatggg ttatatggtt ggtacaagta agaaacgcgt tcgttcatac
    29641 ggtctgctgc cattgtgtat tgaatcaaat cgttcgtacc tacagagaag aaatctactt
    29701 cttttgcaaa cttgtctgct aagactgctg ctgctggaat ctcgatcatg atgccgactt
    29761 ggatcgtatc agatacttcc ttgccttcac tgatcaattt ttgtttttct tcttcaaaga
    29821 tcgcttttgc tgcgcggaat tctttgagtg tagctaccat agggaacatg atacgtaagt
    29881 taccatgaac agatgcacga agcaatgcac gcatttgtgt acggaacatt tcgtcgcctt
    29941 gttcagataa actgatacgc aatgcacgat atcctaagaa tggattcatt tcatgaggta
    30001 attgcagata tggtagttct ttatctccac cgatgtccat tgtacgaaca actacaggtt
    30061 ttccttccat accttctaat acagctttgt aagcttcata ttggtcatct tctgttggga
    30121 agtctggaga atccatatac aggaattcag tacggtacag accaactgct tctccaccat
    30181 tattatggac accaactaga tcttttggtg ttccgatgtt tgcagctaat tcaaaatgct
    30241 tgccgtcagc tgtgacagtt tctgcatttt ttaatttttc ccattctgct ttttgtgctg
    30301 cgtaatctgc acctgctttt tcaaattcag ctttttgttc atctgtagga tcgatgatta
    30361 catcgccttc gatcccgttg acagccaaaa tatcgccttc tttgacttta gcagtaattt
    30421 cttttgtacc aacgattgct ggaatttcta aagaacgtgc catgatagca gaatgtgaag
    30481 tacgtccacc aatgtctgtt acaaacgctt ttacaaaatt gcgatctaat tgtgctgtat
    30541 cacttggagt caagtcatgt gcaacaacga ccacttcttc gttgatcatt gatggatttg
    30601 gtaaagtaac gcctaataag tgcgctaaaa tacgtttagc aacatcacga atatctgcag
    30661 cacgctcttg catataagca ttatcttcca ttgcttcgaa cataccgata tacatatccg
    30721 ttacttcttt tagcgctgat tcagcattta cgctgttgtc tttaatattt tgcttgattt
    30781 gacctaccat ttctggatct gaaagaacca tcaaatgggc atcaaatact tgcgcttctg
    30841 cttcacctaa gctttgcgct gctttttcac gaatttgctg caattcttca gtagatttag
    30901 ctaatgcagc atccaaacga gcttcttcag ccgcagtatc ttctactgtt gccttactga
    30961 atgttaaatc cggttgaact agcaggtaag cttttgcaac ggctacaccg tcactagcgg
    31021 caatcccttt tagcatttca accattattc agataagcct tctttcttca ttgtttcaac
    31081 gattgctgcc attgcatcag cttcgtcagc accttctgca gtaatagtaa catcagaacc
    31141 ttgacctaca cctaaagaca taacgcccat gattgatttc aagtttactg atttaccttt
    31201 atactctaag tttacatcag agttaaattt gcttgctgtt tgtactaata gtgtagctgg
    31261 acgagcgtgg atcccagttt ctgctactac gtgaaattct ttcttttcca tattaatcgg
    31321 tctcctttaa gtgagtgttg tttttatgtg agggttttct tttttagtaa ttcagagaat
    31381 cctaacaaat gccacccttt catctgtaaa gattaacatt ttttttcgtt agatacaaca
    31441 gttttcattc aatttcagat aaaagtattc aaattattcc ggattctttt tcatcctttt
    31501 cttcacattt tataacaaac gtgtttattt tctttttctt gtcgctttct cttctcctga
    31561 cgtttatcat tctttccttt gaccctcttt gttttcatat gaaacgaatt acttgcttcc
    31621 ctttttattt ttagtataat aagagtcaaa ggtcaggaat ggtcaataga gatttcacct
    31681 tttctacctc ataaatctct taaaaaacta ggggttattc ttttacctca tgaaaaacga
    31741 gatgtaaaat atggatgtac ccatttttgt gaaaggacgt gaatgattca tgctttgtca
    31801 aaactgcggc aagaatgagg caacgattca cttgtatgcc actgtcaatg gtcagcggac
    31861 acaactcgat tactgtcaga gctgttatca aaaaatcaaa aatcaacaaa atggaggtct
    31921 gactaatatg gcccaaaatg atccatttgg atttggcagt ttggacgatc ttttccgttc
    31981 catgtctcgc caaatgcaac agcaaggaaa ttatgagcaa acaccaccaa cacaatttgg
    32041 cggaaacaac aacttcaatg gtggtcagcc acctcaaggc aatacagaag gtttattggg
    32101 agaatacgga atcaatatca cagaacaagc acgacaaggt gatatcgatc ctgtgatcgg
    32161 acgcgatgac gaaatcaaac gagtcattga aattttaaat cgccgaacca agaacaatcc
    32221 tgtcttgatt ggagaaccag gtgttggtaa aacagcagtt gttgaaggtt tagcacaaaa
    32281 aatcgtagat ggtgatgtac cgcaaaaatt aatggacaaa gaagtcattc gtttagatgt
    32341 cgtatcttta gtccaaggta ctggtatacg aggacaattt gaagaacgta tgcaaaaatt
    32401 gattgaagag atccgtcaag cagaaaatgt gatcctattc atagatgaag ttcacgaaat
    32461 cgtcggtgct ggttctgctg gtgatggaaa tatggatgct ggtaatattt taaaaccagc
    32521 tttagctcga ggagaattgc aaatggttgg tgctactacc ttaaatgagt accgcatcat
    32581 cgaaaaagac gctgctttag aacgtcgtat gcaacctgta cgggtagatg aaccaactgt
    32641 cgaagaaacg atcagtattt tgaaaggttt gcaaaaacgt tatgaagact atcatcatgt
    32701 aaaatatact gatgaggcca tcgaagcagc cgcaacttta tccaatcgtt acgtccaaga
    32761 tcgcttcttg ccggataaag ccatcgattt gctagatgaa acaggttcta agaaaaactt
    32821 gacgatccaa atcgttgatc ctaaaacaat cgagaaaaaa ctgcaagaag cagaagaaca
    32881 aaaagtttta gcttctcgag aagaagattt cgaaaaagca gcttattatc gcgatcagat
    32941 caataaactt caaaaaatga aagaacgtca gctcactgaa gaggaaacac ctgtgattac
    33001 cgaaaaagat atggaaaaaa tcgttgaaca acggacaggt attccagtgg gtgaattaaa
    33061 agaaaaagaa cagacacaac tgaaaaactt agcagatgac ttgaaagctc atgtcatcgg
    33121 acaagataac gctgtagatc gagtagcgaa agcgattcgc cgaaaccgtg ttggattaaa
    33181 caaacagaat cgtccaatcg gctccttctt atttgttgga ccaactggtg taggtaaaac
    33241 agagcttgcg aaacaacttg cctacgaact attcggttca caagattcaa tgattcgttt
    33301 cgatatgtct gaatatatgg aaaaacatag tgtctctaaa ttgattggtt ctcctccagg
    33361 ttatgtcgga tacgaagaag ctggtcaatt aacagaaaaa gtacggagaa atccttacag
    33421 tcttgtcttg ttagatgaag tagaaaaagc tcatccagat gttttgcata tgttcttgca
    33481 aatcttagat gatggtcgat tgacagatgc acaaggaaga acggtcagct ttaaagatac
    33541 gatcatcatt atgacaagta atgccggaac tggtaaagta gaagcaaacg ttggttttgg
    33601 tgcggcacgc gaaggtgtta ctcgttctgt attgaaccaa ttaaacaact actttacacc
    33661 agaatttctg aaccgttttg acgggatcat cgaattcagt gcattatcaa aagaaaactt
    33721 gatgacaatc gtcacactaa tgctagacga tgtcaaccaa atgctggcag cacagcaact
    33781 tcacatcgaa gttcctacga atgtcaaaga aaaattagtt gacttaggct atgatccatc
    33841 tatgggtgct cgtcctcttc gacgcacgat ccaagaacaa atcgaagatg gtatcgctga
    33901 attttatctt gatcaccctt ctatccatga gttgaaagca aaactggata aagatggaaa
    33961 aatcatcgtt acttctaaac cagaacgttt ggctaaagaa tcagccgaag aaacggctga
    34021 ataaatccag ttttatagcg tgagacggta tttctttgag aaatgccgtc tcattctttt
    34081 ttcagaaaag tcaaaatact gttcatttta taaaaaagaa attataataa gagaaacaag
    34141 caattaaaaa tacataaaag aggtgagcgc catgaaattg gtaaatgtaa caaacagcca
    34201 ttcccgtctt gtattaaacc agcttgaaaa cactgatgcc catctagtca aggtctacac
    34261 agccggaaat accactattg tctacactga agcccctgaa cataatgaaa ttctgctgat
    34321 caatgacaaa cgtaaaatcc agccgaaaga aatcgaagat gctaaaaatt atttcttaga
    34381 taaaatcaaa gacgccgttt accacgagga tgaaatcaaa gtaatcgaat tggcaggcct
    34441 tgttgaaatc tcgattccaa aacatgaatt aggcgctaca ttagccgtgt aaacaaattt
    34501 tgaacataat aattaaagag cgtgggacaa aagtaaagaa cacttttgtc tcacgctcta
    34561 aacacgtata aacggcggaa gcagaagcaa ctccttcgga aataagctaa aattcacgaa
    34621 aattgctttt caaatttttg tgaattccag cttatttctt ggagttaaac gcttctgtct
    34681 cagcctcttt ttctttctag ctttttgatt tttctctttt cttttgtact tttctctttt
    34741 cattttcagt tctacgcgtg tctttatttt taactttttc ctctttatgg atgattgatc
    34801 gtactttttg tgcaatttgc tcgtttttgt cccagtcttt taaatctaat aatcgacata
    34861 atcgtaacat ctcttctttt ttctgttttt ttctttcacg tatttgccat tctgtttcca
    34921 tgtgtcacac tctcctttct ttaatcatat gtacgcaaaa taatttttat atttttttga
    34981 ttagacattt ctatgatttt aatatagtaa aaagagccgt cacggaaaat ccgtcacggc
    35041 tctaaacaaa atatgagaga ctgtttttgt ttggctattc ttattacttg atgcagggca
    35101 gcttgtgtca agacttcttt acatcaaact ttttaatttt acattaggat atttatccgc
    35161 aaaccagcgt tctgcaaatt ggttctcgaa taagaacaat ggttggtcga aacggtcacg
    35221 agccaagata ttgcgacttg aactcatctt ctcatccaaa tcttctggat cgatccagcg
    35281 agcgattttg tgtcccattg gagacatgat tacttcggcg ttatactcat ttaacatacg
    35341 atattggaat acttcgaatt gtagctgacc aacagctcca atgatatatt cttccgttag
    35401 atatgtttta taaagctgaa tcgctccttc ttgaactaat tgatagattc ctttgtggaa
    35461 ggatttttgt ttcatgacat tttttgcagt cactttcata aacagctctg gagtaaatga
    35521 agggagttct tcataagcaa cttttagttt tccttcgaac aatgtatccc caatctgata
    35581 attgcctgta tcatatacac cgatgatatc gcctgctact gctttttcga cgttttctct
    35641 cgaatctgcc ataaattgtg tgacattgct taatttcagt tttttatttg tacgttccaa
    35701 ccagacatcc attcctcgtt cgaatgttcc agaacagatt cggacaaaag cgattcggtc
    35761 acgatgggct ggattcatat ttgcttggat cttgaatacg aaacctgaaa attcttcctc
    35821 ataaggactt accatctgat catcttgtgt tttgtgtcca taaggagctg gtgccaattc
    35881 aacgaaagtc tctaaaaaag tctgtacacc gaagttagtc aatgcagaac caaaaaatac
    35941 tggtgtctga tcgccacgag caattttttc ttgattaaat gcatctccag cctctttgat
    36001 tagttctact tcttctaaga cttgttcata taaggaattt tcagttagag gatgattttc
    36061 gggaagcagt ccatcttcat ccaattctac taatcgttca tcctgataat tttccggacg
    36121 ataaaactct acacgattat ggtagatatc ataaagacct tctagccctt tacccatacc
    36181 gattggccaa ttcatcggat aagattcgat atctaatagc tcttctaatt cttctaacaa
    36241 ttccaatggt tcacgaccat cacgatctag tttgttgata aaagtaaata tcgggatgcc
    36301 acgttttttt acaacttgga ataatttctt tgtctgtgct tcgatccctt ttgcactatc
    36361 gattaccatg accgcactat ccacggccat caaagtacga taagtatctt cagaaaaatc
    36421 ttcatgtcct ggtgtatcca agatatttac tcgtttccct tgataatcaa actgcataac
    36481 agaactagta acagaaatcc cacgttgttt ctcgatttcc atccagtctg atttggcaaa
    36541 attacctgtt tttttgcctt ttacggtccc ggcttgacgg atagccccac caaacaatag
    36601 taattgttct gtaatcgttg ttttcccagc atccggatgg gaaatgatcg caaaagttct
    36661 gcgattgtcc acttgttgct ttaattctgg attattcata acctaactcc atttcttcca
    36721 ttcaattatt tatattttct ctaggcacag attgtccgat ctcttaaaga aagacaattt
    36781 catatagggc tgtgacaaaa gccgtgtagc agtctctttt tccgagtaaa cggcgttcta
    36841 gagacagcct atcagcaata agcccaccca agccaaaaat acgaaaagct gtttttgctt
    36901 ggctgctctt attacttaat gcaggatagc tttttcacaa cctttgtcaa ctactacaac
    36961 tgaatcagta tagcatggat tcatcgaatg aaaaagccac caaaccataa gagtcaaata
    37021 tttttttgtc agctgactaa aagaaaagac gtgttgcttg tttattcaag caacacgtct
    37081 ttcataacca agtttccaat tatcttcttg gctcgtcatc ttcgaattct tttctgaagt
    37141 atcttcgttc ttcttcaggt tccgcttctg tttcttccgg atcatgaaaa atgacacgga
    37201 ttttaaggat tcgagaacct tccatttctt cagaaaccag cgtaatatta tcgacatcaa
    37261 acgataactt ttcgccttca tccggaatca tgcctaatgc tgtaatcaaa taaccagcca
    37321 tcgtatctac atcactcata tgcagatcac tgtcaaaagc ttcattgaac tcatcaatca
    37381 gcatacgtcc ttggatgata tattcgtgtt catcgatttt ttcataaaga ttctcaactt
    37441 catctgattc atcatcgatt tctccgacga tttcttctaa aaggtcttct agtgttgcta
    37501 acccaacgac gccgccatat tcatctaata aaatagccat ctgattctgc gtctttttca
    37561 tttcatataa cagatcatca ataaagaccg tttcaggaac gaataaaggc tcctgcatga
    37621 ttttcttgat atctaaatta tcaaaaccga atttatgggc agcttttagt aaattctttg
    37681 tatgaaggat tccgactact ttatctttgt cttcgttata aacagggatt cttgaatagt
    37741 tttcggataa aacttcattc acattttctt ccacagtgtc gttgatatca atcataaatg
    37801 catccgttcg tgggaccatg acttctcggg caactttcgt atcaagagaa aaaacccctt
    37861 ggagcatttc taattcttca ttattcaata cgccttcgtt ttcaagcata taacgcattt
    37921 catctctggt catttttgaa tcttcatcat caaaagtcat cggcgtgata cgcgccaata
    37981 aacttgtgga agcagaaagc agccagacga agggcttagc tactacacca atcaagcgta
    38041 tcagccctga agtgaacttt gcgacttctt ctgatttatt taaagcaata cgtttagggt
    38101 acaattctcc aaaaacaatc gatacatagg tcaaaatagc cagcacaatg atatttgcaa
    38161 tattttttgc cgctgtgccg ccacctaata ccggagctaa ccgttccgat aaagtattag
    38221 ctagtgaagc accagacaaa atgttgacca aagtgattcc tacttggatt gtcgatagga
    38281 aattatttgg attctgcaat atttttaaaa gcttttgaga tttcacatct ccttcatcag
    38341 ctttttgctc gattcgattc ttattgatcg aaacaacagc aatttcggaa gctgctaaaa
    38401 aagcattgat caatgtcaaa acgatcaata ataaaatctg cgctaacagc gactgactct
    38461 cagggtcagc attcataatc gcattctctc cttataaaat taatttttag tttacgatag
    38521 aaaaaaacgt ggcactatag ccacgttaaa atggtatcac aaagcaattt tctttgcaat
    38581 aaaaacaagt gtactaaaac tacctacgag gtcgtagcaa ttttcttctt gttttcattt
    38641 tgcttgtctt ctttgacgat tttgattata taggaaatga tcgtcttgca gattgcgtaa
    38701 aaaggaacac ctaagaagat accaagcaat ccggcaatat ttccagctac tagaagaacg
    38761 ataatgatcg ttaatggatg gattttcagt gatttcccaa ttacattagg ataaatgata
    38821 ttcccatcca actgttggac gattagcaca accacacaac aaagtaatgc ttttaccgga
    38881 tcattgaaga ctgttactag aaatgcaggt gctagtccta gataaggtcc taaatatgga
    38941 attaggtttg tcaatcccgc aatgacccca aataagaaag cataatccac accaattacc
    39001 atatatccta aaaatgtgaa ggtaccaacg aataaacatt cgattgcttg tccgctaatg
    39061 taattagcca atgttttatt cagttgcccc aataactcca caatttggtt tcttcttttt
    39121 tctgggaaaa actgttggat gtttggcact agtttttctc cgtctttcaa catatagaac
    39181 aagatgaatg gtgctgtaat gattactacg gttgccgaag cgatcgttcc aacgatcgaa
    39241 cctaaactat tggatactcc actaaggaat tgctgaatga ttgtcccgta agaaatgtct
    39301 aattgctcaa aatatttatt gatatctact tccttgaaca aaggcaattc ggctacctct
    39361 tttaaccagc tttctacctg tttgatgaaa tcaggcatat tagatgccaa tgaggaaatt
    39421 tgctgtacta gacttgggat gacactaagt aaaatccaaa taattgctgc aactaaaagg
    39481 agcagcacaa ttgcgacagc ccatattctt ttgatttttg ctttcatcaa cagcttgaca
    39541 ataggattca ataaatagta aagaaagcct gcaatcagta caggtgcaaa taacgtggaa
    39601 aaaaatgtcc cgatcggttc gaaaataaag tttatttctg atgaaatcat gatcaatgca
    39661 gctatgatca ataattcaag cgaccaaaac attagtttag actgtctaag tttttcaaac
    39721 aatagatttc ctcctttctt tcttactagt atatattat
//
LOCUS       DAIGOK010000008        38187 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.008, whole genome shotgun sequence.
ACCESSION   DAIGOK010000008 DAIGOK010000000
VERSION     DAIGOK010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.008"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(77..739)
                     /locus_tag="NOA01_000311"
     CDS             complement(77..739)
                     /locus_tag="NOA01_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCI0631543.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            923..2569
                     /locus_tag="NOA01_000312"
     CDS             923..2569
                     /locus_tag="NOA01_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCI0631544.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            2560..3642
                     /gene="uxuA"
                     /locus_tag="NOA01_000313"
     CDS             2560..3642
                     /gene="uxuA"
                     /locus_tag="NOA01_000313"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCI0631545.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDIGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(3750..5303)
                     /locus_tag="NOA01_000314"
                     /pseudo
     CDS             complement(3750..5303)
                     /locus_tag="NOA01_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            5451..6592
                     /locus_tag="NOA01_000315"
                     /pseudo
     CDS             5451..6592
                     /locus_tag="NOA01_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(6718..7797)
                     /locus_tag="NOA01_000316"
     CDS             complement(6718..7797)
                     /locus_tag="NOA01_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCI0631546.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(7900..8340)
                     /locus_tag="NOA01_000317"
     CDS             complement(7900..8340)
                     /locus_tag="NOA01_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCI0631547.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(8397..8807)
                     /locus_tag="NOA01_000318"
     CDS             complement(8397..8807)
                     /locus_tag="NOA01_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCI0631548.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(8987..9421)
                     /locus_tag="NOA01_000319"
     CDS             complement(8987..9421)
                     /locus_tag="NOA01_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCI0631549.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(9521..10369)
                     /locus_tag="NOA01_000320"
     CDS             complement(9521..10369)
                     /locus_tag="NOA01_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCI0631550.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            10758..12524
                     /locus_tag="NOA01_000321"
     CDS             10758..12524
                     /locus_tag="NOA01_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HCI0631551.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            12626..13885
                     /locus_tag="NOA01_000322"
     CDS             12626..13885
                     /locus_tag="NOA01_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCI0631552.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            14032..15333
                     /locus_tag="NOA01_000323"
     CDS             14032..15333
                     /locus_tag="NOA01_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCI0631553.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            15336..16187
                     /locus_tag="NOA01_000324"
     CDS             15336..16187
                     /locus_tag="NOA01_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCI0631554.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            16200..17015
                     /locus_tag="NOA01_000325"
     CDS             16200..17015
                     /locus_tag="NOA01_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HCI0631555.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            17166..18332
                     /locus_tag="NOA01_000326"
     CDS             17166..18332
                     /locus_tag="NOA01_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCI0631556.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            18562..19209
                     /locus_tag="NOA01_000327"
     CDS             18562..19209
                     /locus_tag="NOA01_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCI0631557.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            19293..20237
                     /locus_tag="NOA01_000328"
     CDS             19293..20237
                     /locus_tag="NOA01_000328"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HCI0631558.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            20372..21823
                     /locus_tag="NOA01_000329"
     CDS             20372..21823
                     /locus_tag="NOA01_000329"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HCI0631559.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            21844..22680
                     /locus_tag="NOA01_000330"
     CDS             21844..22680
                     /locus_tag="NOA01_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCI0631560.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            22680..23339
                     /locus_tag="NOA01_000331"
     CDS             22680..23339
                     /locus_tag="NOA01_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCI0631561.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            23465..24154
                     /locus_tag="NOA01_000332"
     CDS             23465..24154
                     /locus_tag="NOA01_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631562.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            24160..25116
                     /locus_tag="NOA01_000333"
     CDS             24160..25116
                     /locus_tag="NOA01_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HCI0631563.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            25160..25672
                     /locus_tag="NOA01_000334"
     CDS             25160..25672
                     /locus_tag="NOA01_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCI0631564.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(25928..26476)
                     /locus_tag="NOA01_000335"
     CDS             complement(25928..26476)
                     /locus_tag="NOA01_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCI0631565.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(26565..27746)
                     /locus_tag="NOA01_000336"
     CDS             complement(26565..27746)
                     /locus_tag="NOA01_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCI0631566.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(27982..29490)
                     /locus_tag="NOA01_000337"
     CDS             complement(27982..29490)
                     /locus_tag="NOA01_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HCI0631567.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            29649..29840
                     /locus_tag="NOA01_000338"
     CDS             29649..29840
                     /locus_tag="NOA01_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631568.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            30043..30960
                     /locus_tag="NOA01_000339"
     CDS             30043..30960
                     /locus_tag="NOA01_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HCI0631569.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            30953..32026
                     /locus_tag="NOA01_000340"
     CDS             30953..32026
                     /locus_tag="NOA01_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631570.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            32174..32692
                     /locus_tag="NOA01_000341"
     CDS             32174..32692
                     /locus_tag="NOA01_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631571.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            32673..33065
                     /locus_tag="NOA01_000342"
     CDS             32673..33065
                     /locus_tag="NOA01_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HCI0631572.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            33141..34076
                     /locus_tag="NOA01_000343"
     CDS             33141..34076
                     /locus_tag="NOA01_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HCI0631573.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            34209..34988
                     /locus_tag="NOA01_000344"
     CDS             34209..34988
                     /locus_tag="NOA01_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCI0631574.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            34981..35298
                     /locus_tag="NOA01_000345"
     CDS             34981..35298
                     /locus_tag="NOA01_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCI0631575.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            35542..37152
                     /locus_tag="NOA01_000346"
     CDS             35542..37152
                     /locus_tag="NOA01_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCI0631576.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            37309..38157
                     /locus_tag="NOA01_000347"
     CDS             37309..38157
                     /locus_tag="NOA01_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCI0631577.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        12579 a         6785 c         7423 g        11400 t
ORIGIN      
        1 ggatattttg ttatttaata aagattactc aaaaacgaag gttaattata aaaatagaag
       61 aggtcgcaag aaaatgctac tgctggaaat actcgggata acgttgagta acagtttcct
      121 gttctttcag catcatgtgt aagtgcaatc ccgtttgaaa tcgagcttct tctggttttt
      181 tatcaactaa agccttgagt aaatcataat gttgttccat gatcgtttcc catggaagcc
      241 cctgaatggt cactcgtaac cagcggaaac gttctaaatg ggtattatgg tcatctaacc
      301 agttccaaac ttcttttctt cctgcaattt cgaaacaagc ttcatgaaat aaattatctg
      361 cacggaaaaa agcgcgttcg tcatttattt tgactgcttt ttcttgttct tctaaaatcg
      421 tttctagcac tattcgtgtt tgtgtgtcca atttggaaca acattcaatc atgatttgct
      481 cttccaactg ttctcggaca aagcgagcat tttctgcaga aacaagatta attttggaaa
      541 catacgtacc actttgaggt atcgtataca cgagttcttg ctgacgtagt tgaatcaatg
      601 cttcgcgaat gggagtccgg ccgatagcaa gatgttcctc cagtgccttt tcagatattt
      661 tccttccagg ttctaagtca gcatagataa tttgtttacg aatcgcttga taagcttgtg
      721 attgcaagct gcttttcatc tttattcctc caaaatcatt tctatgaacg tagtataaca
      781 gaagaggtct gaaaaacaat ctggtatgtc acattaacta gtaaatttgt ataaatagta
      841 aaattttggt tgacatagcg atttttattg cataaaattc acttgaatac aaaaactaat
      901 atgttagttt aggaggggaa aaatgattga tttaaacaac tggaggaact ttatccctga
      961 attatctcaa aaagaagtag aaatacctca ttttgaccgt gcatcattaa gagaaaatgg
     1021 acaaaaaaat cctgtctgga tccatttcgg tggcggaaat ctttatcgag gatttcatgc
     1081 agaaatcgct caacgtttag cggatctagg cgaattgtcc actggtgtca ttgtttgtga
     1141 aacattcgat gaagaaataa ttgaaagggc ttaccaagat tttgatgatt tgattcttga
     1201 agtggtcatg ccaagcagtg gagagttcgt caaacgtata ttagctgcga cagctgacag
     1261 tctgtattgt catccagatt gttctgaaca ctttgataag atgattcgac actttgaaaa
     1321 tcctagttta caattagtta cttttgctat tactgaaaaa ggttatcgat tgaaagatac
     1381 aaataataaa tatataggac cgattgcagt cgatataaaa aatggtccaa agaagccatc
     1441 acatacaatg agtattcttg ctgtgctact ttataaacgt tttttatctg gagcttggcc
     1501 gattgcactt gtctctacag ataatttttc acaaaacggt agtcaattac gtacttcttt
     1561 gctaacgatc gcaaaagaat ggcaaaagga aggttatgta caaaatacat ttatccaata
     1621 cttatcgaat gaagaaattg ttgcgtttcc atggacgatg attgatcgta tcaccccgaa
     1681 tccttccttg tcagttagca aaatgttggc aaaagagggc ttttcaaata tgtcgatttt
     1741 ccaaaccgca aaaggaaata atattgctcc atttgtcaat acagaggaca tccattattt
     1801 aatcatggaa gatcattttc ccaatggacg tccgaaattc gaaaaagctg gggtattctt
     1861 gactgatcgt gcaacggtag ataaagcaga tacgatgaag gtgacgactt gccttaatcc
     1921 tttgcatacg tctttagcta ttatgggttg tttactgggg tataggagca tagctgagga
     1981 gatgaaagat ccagatttat cgctgctagt ttctgaaatc gggtacaatg aaggcctccc
     2041 agttgtggag tctccaggta tcattgatcc aaagatcttt ctggaagaag taattactgt
     2101 gcgcttttct aatccaaata ttcctaatat gcctcagcga atcgccacgg atacctctca
     2161 aaaggtggcg attcgttatg gtgagacgat taaaaaatat atggaaatag aagttaaaga
     2221 cccgcagaca cttgtattca taccattgac tctcgctgct tggcttcgat accttattgg
     2281 cattgatgat caaggaaaac cattcatccc gagtccagac ccactattga cagaccttca
     2341 gcataagctt tccgatattc aactgggtac gcaagataca tcacatattc atgagaaaat
     2401 ggctcctatt ttgtcaaata aagaaatttt tggtgtagat ctttaccaag cagagcttgg
     2461 cagaaaaata gaagaaatgt tcatcaagat ggtagcagga ccaaatgccg tacgaaaaac
     2521 attacaggat tacttacaag aacatggagg aaatttatca tggaaatgac ttttagatgg
     2581 tacggatcac aggaagaaaa gattacctta gatgaaatca gacagattcc tgcggtcaaa
     2641 gggattgtag gcacattgat ggatatacca gtaggagagg tatggcctag ggagaggatc
     2701 aaagaactaa aagaagaaat tgagtctcgt gggttaacac taaaagtaat tgaaagcgtc
     2761 aatgtgcatg atgccattaa aattggatta ccttcccgtg agcagtatat cgaaaactat
     2821 aagcagacaa tccgaaactt ggcagaatat ggaatagaag tcatctgcta taattttatg
     2881 cctgtatttg actgggtgaa atctgacctt gactaccgct tagaagacgg ctcatccacg
     2941 ttagctttta tatctgatga tattccagat gatccaagag aaatcgtcgc gcgcatcgaa
     3001 cattccagca atgaatttgg attgccggga tgggaacctg agcgcttagc tcctatcaaa
     3061 tccttgcttg aagcctatgc atcagtagac gaagaaaaac ttcgagaaaa ttttgcctat
     3121 tttctccaat caatcatccc aacatgtgaa gaggtagggg tcaaaatggc tgtacatcca
     3181 gatgatccgc catactcgat ttttggatta gctcgaatcg taaagaatcg agaagattta
     3241 gattggttgt gtcgcatagt agacagccct tataacggaa tcactctttg tacagggagt
     3301 attgcagaag atccagaaaa tgatgtatat gggattttag cagaattttg ccaaagagat
     3361 cgaatcccat ttgcacatgt tcgaaatatt aaatttttag ctaatcgaga tttctatgag
     3421 gcaccacata aatccgaata cggttcatta gacatgtacc gcatactaaa aacaatgtat
     3481 gacatcggat ttaacggata catccgtcct gatcatggaa gaatgatctg gaatgagaca
     3541 ggaagaccag gatacggctt atatgatcga gcattaggtg ctgtttatct aaatggatta
     3601 tgggaagcaa tcgaaaaagg aaacaaaagt aaagataatt agtgaaaaaa actgtaattt
     3661 tattatgtaa ataagaagga tgttcaaatt cttgatacat aaatccggag gttgttaata
     3721 agcattcgga tttatgtatc ttatttggtc taggaagtaa cttgattttc ttttttcgat
     3781 tgatccatga gtgcttcttc ttttaattca aagatatatt ctgagatcct cataatcact
     3841 tttttccagt cttctggtgt gatgattcct tttaaaatat agattttttt gatatgagaa
     3901 ggcaattgtt tcaaagggca gtcgctgata acgatgttgc agttctccat aaaatcattt
     3961 tgtattttga tcgattcatt tgaaaaaatt tgttctatct taacgatcag atctttactg
     4021 atataaaaat cacgcgtata gttcacacca atcacaattc gcggctgttt tttgtagcga
     4081 tcatacaaat gtactaggtc ttcaaccatc cattgcacat tttccttttc taaaataact
     4141 ggtaacaagt gttgattttg ttctctgaaa tccatataaa attgttcaat ggcggtttgg
     4201 acttctttca atacgctatg ttcttgataa agcatgaaag taggaagggg cgtatttggt
     4261 tgattgaagt taagaaatcc attataaagt ttatttcgtg cgaatgccaa tgagtaaaga
     4321 atcttttctt tttcaggtat aaacaaatca aatacagtac aaagggactc caccatttct
     4381 cttgaataaa gaacatgagg aacgtttgct ttttttaaaa gtttgtattg ctccaagctt
     4441 gtttcttccg attcgatttt cgtgatagaa agtcgagcta aaatattcac taagatgcgt
     4501 tgttcgcgac aaattggcag attaggtcgc aaaatatcta cagagagatc aataaaaaag
     4561 tcaatatatt catactctgt gaattctttt tgtacgtctt tcacagaagt tgcaggaaaa
     4621 ttctctttac atatttttaa tagaaaatat aatttatcta tcataccgct atttagtcgg
     4681 tcaagtactt cttttttaaa aaaagtattc atcataaaag ccactacttg cggatcttca
     4741 tcataaaatt ctgtagcagt acttgaaaag atgttccaat aaatatctaa tgctgcatat
     4801 tgaatttgta cctcttttcc agaaaaagac tttctgccat tattagaaaa ggagatagac
     4861 aaaccgtact atgctaaaaa tgcattaatc tttttcattt tcgtatacaa ataagaggca
     4921 ctatagtttg tttgctgact gaaactgata atcgaatttg tctgacagct gaacaaagaa
     4981 gtcaagatct gataactgac tgattcgttg cagtatcttc caaatagacg ataatagcag
     5041 taagccggat caattccttc aaaattcaac tttaaaaaag gtttttgctt atcaatatgc
     5101 agttctgtta gattgagacg ctcaaagtct tgttctaatt ctttgatgta tagtaaaaaa
     5161 tttgattcag acatatggaa atgatctgct aaatattgtg tagtaataaa gaaacgatgt
     5221 tcagatagga aaagaaaaat atctaatttt gtattgcttt ttttcttcaa gataaacgtt
     5281 ttaatcgctt cttgagtttg catgaaagag gctccttttt acttaaattg ttaaaacttg
     5341 aaaatattac tgtttttttc ataaatttag acaaaaaaac agtaaacata taggtagaat
     5401 ctctactatc ttatttcttg catacaaaaa aatcaaggag tgagagagtt atgagatatt
     5461 caaccatctt aatatcaaca tttgcagcct taagcttatc gcttactata aatcagacag
     5521 tccacacaga ggagacagaa acagccaaat ggacagaaac caagtgggcc aacgtcaatc
     5581 gaattacctt tcaggatgaa ttttcatcct tcaattcagg agtcgcaaaa ttaagaaaag
     5641 gacaaacagc gaccatcaaa gcaaaaattg actattcggg agacttagca cctatactac
     5701 aatcagctgt tacatttgag aatgttgatc ccactttgtc gattggctat gacgagaaca
     5761 ctttgactat ccaacacaag caagcgatat ttaccttcac cgttacatta aatcaggact
     5821 tgttaaaacc agcgcttttt actgtcaagg tatcagatgg actaccgaac gatcatcatt
     5881 taacgccgta tagtcagcgg cagacaattg agcaagattc agccatcgac tcaggaggag
     5941 atcttgttga agaaccaaca gataaaccag aaaatgaaaa taagccagaa gtaccgccta
     6001 cagaaaatcc agacggagaa cagaaaccgg aaatagaacc tggagaagaa cccgatacag
     6061 aaactcaacc agagccagac aataaatcaa aacctgaaat aacccctgga gaaaaaccag
     6121 atgtaactcc agaaccagat acggattctg gaaaccaaac agtaccggaa acaaatccag
     6181 acacagataa tgaaactgaa aatccagaaa aaccagaggt agatccagaa gaaaaaccag
     6241 atgtgactcc agaaccagat acggacgcaa gagaccaagg aataccagaa aaaataaaca
     6301 aaaaaacaat tcaagaggat ggaaaaaaag agagtaaaaa atcaaatcta gcaattctaa
     6361 aaataaatga agagcagctg aataaaaaat cacgaatatt tgatagcgcc caatcagctg
     6421 aaaccttaaa aagtagcaag gatacaacct ttgctgtcct gaaacgaaaa ataaacagct
     6481 accaaaatcc ggagagtcac aaaacaaagt tatactgtgg agcggaatca ttctattaag
     6541 tatagcaaca atgctttctg caaaaagatt taaacaaaac agatctctct aacatttgaa
     6601 taatgtctgt gaacgcattt ttcaaataaa aagaaatatc cagaaaatga ccccaaaata
     6661 ccagattgaa atctgatatt tggggtcgtt catactttat ctttcttcat atttatttta
     6721 agggatatct gtttcttttg gaagatggtt cgggatcttt ttcactttca atacatctaa
     6781 aaagaaggta aagatcggaa tgccgacaat caagccccaa acgccaaaga accgttcgct
     6841 aacaagtaag accacaaatg tataaaaaat cggcagttct gttttgctgg acatcaactt
     6901 cggattcagc acataagact caattagatg gacaatcaaa atcgtaatca ggatataaac
     6961 aacatctcgg atgcctcctt gggaataagc tatgaaagat agaggaatac aagaaatgat
     7021 aacacctgct actgggatca gacttaaaat gaaaatcatg atcgctaaac tcagcagctg
     7081 atgaaagcca aatacagcca aggcgattgt agtgagcaac gtatttaaaa gagcgacaac
     7141 aaattgagct tctaatacca aaccaaatgt attgacaaac ttatcggcaa aatagtaaat
     7201 atcttgaaag aaccaagaaa attcactttt taaaaacaat cttgagaata caattgtttt
     7261 tttcttttca atcataaaaa agaaacttaa gatgaatgac atagcaaacg ccactgccat
     7321 ttttcctata tcctgtaaat agccaagcgc aatcgaagcg ccattttgaa gctgttcaaa
     7381 aaaatcgttt cgctcaatat aagtatggat atattgtaaa acaggatctg catcttttgg
     7441 ctgatgctcg taaaaattaa taacggactg gatcatgtca aatgtttgat gaataagcat
     7501 cgggatatac tttgtgaatg ccagatagat aaggtaaacg atcagtgtat aaatcatgat
     7561 agctaaaaca cctgtaggga ttcggacaaa tcgctgaatg atttgaacaa gatgaatcat
     7621 caaataagta aagataaatg tgagcaaaat ggttgtaatc atacttctaa ctaagaaaag
     7681 aacaaaaacg acaaataata aaacagtgag acggcgtatt ttttcattcg ccaaaaaacg
     7741 ctgataaacg gtcaaatcga caacctccaa ataacaaaaa ataaacacgt tattcattat
     7801 atgatggaat atgaaaataa gaaagagcaa aaagaaaaac cgctaaatga cgttatctta
     7861 tgagttggtt ttcctcataa aatcgttcat ctagcagttt cagccctttt tctggtcttg
     7921 atgaaaatta tctagcattt ttcgggtcag atgttgcagt gtatccaatt cttcctgttc
     7981 aagtggaaga taagacttga tcttttgtgg aataccttct gcttgttttt gcaacgcagc
     8041 accttgagca gtcagcgtaa catttacgat tctttcatca cccgtttcac gctgacgatt
     8101 aatataattt ttttctgcta acttttttag taagggagtc agcgtaccag aatccagaca
     8161 caaatcattt cctagctctt tcactgaagt ctttttcttt tcccataaaa ctaacatcac
     8221 aagatactgg gtataggtaa gattcagcgg cttgaggagg ggagtgtagt gtctaatgat
     8281 ttctttagag gcggcatata aaggaaaaca tacttgatta actaaatata gatcttccat
     8341 ttttccacct ccgctgtttt actatagtgt atcatagcgg atttacacaa aaaagtctat
     8401 tcactaactg ctttcagagt aacttctata tttccagctg tagcttttga atacggacac
     8461 actttatgcg ctgcctctaa taattcttgc gtttcttcca gagaaagacc ttcgatagat
     8521 ccttcaatct ctacacctaa gacaaagctc gtttcacttt ctgaataaag ggaaacttgt
     8581 gcactgacag tagaagcccc agagatattt ttttgctgtt tgatcaattc taatgcactg
     8641 ttaaaacatg cactgaaacc agctgcaaac aattgttcag ggttggttgc attttcgacg
     8701 cgtttaccag gagctgcaat attcaattcg aatgtacgat cgggattatg cacttctcca
     8761 gagcgtccgc ctgtgttgat cattttcgta gaatacattt ttttcataaa gttaccacct
     8821 ttaattatat tttatacaat tgaattttac acaatataat tgtggaaagc aaagaaatgg
     8881 attggcataa gataaataag agaaagcaca tgaaacagac gcgctttctc gaatagcaaa
     8941 catatacaaa aaatatcaga gacaaaaaat aatctcatca taaacattac atatatttct
     9001 caaaaagttg ttttctttct tcaaaaatta cttcacgatg gacctgatga agcatccata
     9061 aataaccaaa aggatctctg aacattgagt tagtcacacc aaagtccatc atttctgtca
     9121 tttcttgaat ctctacacag ccatttcgta atgctttatt atgtgtgtcc tcgatatcag
     9181 agacgatgat attgaaccag atcgtatttg gatgttcagc agtaggagca gtcaaaccaa
     9241 gagacgtgtt ttcatccagc atatggaatc tcgtaccata caaggtaaaa atgacttcat
     9301 tattgcctct agggtaatct gttgcctcaa tcagttgtat ttcatcaaag attgtttgat
     9361 acagttctag tgctttcaaa ctatcattta atacaaaatc gatttccgtt cctttatgca
     9421 taaagatctc ctctcctttt cagtcgaaca gttactagat tcttttatta taccagctaa
     9481 agaaaacgca ttcaaaaaaa tagcggattt acttaccaac tcattcgatt ggatgataag
     9541 taaatccgcg tcccattact tctgctacgc tgctgacaat gacaaaggca cttggatcga
     9601 tttcttctat caatggtttc attcgggaaa attcatgatc agtcacaacg atcaataaaa
     9661 cctcttttga ctttcgatta tacccgcctt ttgcctctag cagggtaagg ccacgtccaa
     9721 tctcagcact taagcgaagg cggatgtctt ccaaataatg ttcactcatg accataacag
     9781 atttctttcg attgatacct gtttcgatat aagtcatgac aattgaagtg attgcaatag
     9841 ataaaatagc ataaagaaac tcttctattc caaaaataaa cagattaaga gaaacaacga
     9901 atgcgtctgt aaaaaataag ccaatcgaag tattcaagtg gaaatatttt ttaaaaatga
     9961 ggggagggat cgtcgtaccg ccgctagaag agttgttttt atataaaatc gcaactccta
    10021 aagcaaaaat cgcacttcca aaaatgacag acaacaaccg atctgaagta atcattgttt
    10081 ctggaactat tgcaatgatt aaaggaaaaa ccaagcttcc gaaaagtact ttaaaaaagg
    10141 gttttttacc taaaaataga agagctagta ccaacataac gatattcaac actaaaataa
    10201 ctatagctcg gtcaaagcct aaagcagctt ccagtaatat tcctagaccg ctgacacctc
    10261 cagcagcaat atgatgaggc cctaaaaaca tattgatagc gactcctaat atgaagaggg
    10321 caaaaataat cttaatgtat gtccatataa attgtctcgt ttctttcatt tattcacttc
    10381 cttaattcat taataatact cttatcttat tataacttac ttttcacctt aagttaactt
    10441 taattaataa aaaaattagg ctagtctaaa ttattatgtt tcattcttct agaatatact
    10501 tctacgccaa aaactgttaa aaaactttta ctttagaaaa ttggagaaat tgatcgctta
    10561 agataaattc aggacactaa atagtacagg aagtagacta aaacttttaa aagttttaag
    10621 tctatctatt tggcaagttt aaaaaataaa aattcggtat caaaaaactg ttaccgataa
    10681 catttttgaa agcgttgtct tttgcttctc tatcacttat agtgatgacg tcatattaaa
    10741 acacaaggag gaaatcaatg gatacagctg caatttatca tcgaccggaa agtgaatatg
    10801 catttctata tacgaataca agatttcgta ttcggcttcg tactaggaaa aatgatatca
    10861 aaaaagtata tgtgctatgt ggagatccat ataccataac aactgaaaaa tggtatcaaa
    10921 aacaacgacc aatgaaaaaa tgtctaagta ccaatgttca tgattattgg gaaattgaag
    10981 tgacgagcga aacaagtcgc ttacagtatg cgttccatgt tgttggggaa gataacacgg
    11041 actgttttta cggtgatcaa ggaatttttc cttatcgaga aaatgtgatt actgaaccaa
    11101 atttttattt tcgaatacct tattttcatc aaatcgatcg attcactatt ccagaatggg
    11161 taaaagaaac agtatggtat caaatttttc ccgaaagatt tgcaaacggg gacaaagcaa
    11221 atgaccccga acacacttta ccttggggaa gtaaagatcc agaccgagaa gactttttcg
    11281 ggggagattt gcaaggtgtg atggatcatt tggattactt gacggacctt ggcatcaatg
    11341 gcatctattt ttgtcccata tttaaagcca catccaatca taaatacgat acgattgatt
    11401 attatgaggt ggatcccgct tttggcgata aaaaactttt aaaaaacttg attgatgaag
    11461 cacataaaag agggatacgt atcatgttcg atgctgtatt caatcatatg ggtgtccatt
    11521 caccacagtg gcaagacgtt ctcaaaaacg gagaaaaatc gatctataaa gactggttcc
    11581 acatccgttt tttcccagta gattcttatc agatgactga cttaccagaa actgctgaaa
    11641 atatcccata tgatactttt gcttttactc cattcatgcc gaaactaaat acagcaaatc
    11701 cagaagtgca aaaatatttg ctggatatcg caacttattg gataaaagaa ttcgatattg
    11761 atggatggcg tcttgatgtt gctaatgaag tcgatcatca tttttggaaa aaattcagag
    11821 aagcagttac agaaatcaaa ccagacatct atatccttgg agagatttgg cattcctctc
    11881 aagcttggct gcaaggggat gaattccatg cagtgatgaa ttatgcattt acagattcga
    11941 ttaaagatta ttttgcaaaa aagaaaatca cagcttcaca aatggtcagt ggaatgaatc
    12001 atcagcaaat gctttacaga gatcaggtga acgaaggaac attcaactta ttagattctc
    12061 atgatactgc caggatcttg accctttgcc aaggtaacaa agaattgatg aagtctgtga
    12121 tggcctttat gtttcttcaa aaagggtctc cttgtatcta ttacgggaca gaaataggga
    12181 tgactggtga agatgacccg gattgccgca aatgtatgat atgggagaag gaggaacaag
    12241 atctagagct attcggtttt ataaaagaac ttgtttctct aaggaaacag ttgtctaaga
    12301 ttatttctga aggttctact cagtggctaa tagtaaatga tcgtgaagat aagctctatt
    12361 tcacacggaa actagaggga cagatcatct atgtttactt taatcagtca aaagaacctt
    12421 gggtagtaga acaggaaaat gaggtgattc ttagtcaaaa ttgtcagttg ttagaagacg
    12481 ggaaagcaga gatacgacaa tacggatttt tgatcatgtg ttgaaaactg ttaccggtaa
    12541 caacaagaaa gcgtttgcaa atgaagattt gtgcccatta taataagggt gtaacagcac
    12601 gttgaataca aaggaggaaa tgaagatgaa aaaaagaagt gtgaaacagt tgggtttagg
    12661 gttaatctct ttaggaatct taggcggatt agcggcttgt ggcaatggta actcttcagc
    12721 agaaggggaa gccgatgaag gaaaaacgtt aacagtttcc gttgattctg gatataaaga
    12781 ttatataaac gaaataaaaa acactttcga aaaagaaaac gatgtgaaaa tcaagctagt
    12841 agaaaaggac atgttcgatc agttggaatc attagcgtta gatgggcctg ctggaaaggg
    12901 acctgacgtt atgatggcag catatgaccg gatcggtgcg ttaggacagc aaggacatct
    12961 ggcggaagta aaactgggaa atgaagcagc ttacgatgaa acagacaaag cccaagtgac
    13021 ttatgatgga aaaatctacg gagaacctgc agtaatcgag acccttgttt tgtactataa
    13081 caaagattta gtcgatacag caccatcaac gtttaaagat ttggagaatc tatccaaaga
    13141 cagtcgtttt gcttttgagt cagaagcagg aaaaaataca ggatttttag ctaaatggac
    13201 tgacttttat tattcctatg gtttaattgc aggttacggt ggctatgtat ttggtgatga
    13261 tggcacaaat ccttcagata tcggtttgaa taatgcaggt gctgtagaag gaatctctta
    13321 tgcaacagat tggttcaaaa atgtttggcc aaagggaatg caagacataa aaagtgcagg
    13381 agacttcgcc agccagcaat tcatgtccaa taaaactgca gcaatcattg acggtccatg
    13441 gcaagcgcaa acatacaaag aaaacaatat caattatggt gtagccaaaa ttcctactct
    13501 aaataatgga cagccttatc aaccgtttgg cggaggaaaa ggatgggtag tcagcaatta
    13561 tgctaaaaac aaagacctct ctcaaaaatg gcttgattat gtaacgaatc aggaaaatca
    13621 agaaaaattc tttgaaatgg taaatgaaat ccctgcaaac caacaagcta gggaaacagc
    13681 aaaggctaaa aacgatgaat taacaacagc tgtgattgaa caatatgaaa cagctcaagc
    13741 gatgccaaac atcccagaaa tgggcgaagt ttggacaggt gcagaaaact tgatgttcga
    13801 tgcagcatca ggttcaaaaa caccaaaaga atccgcagat gaagcagtaa aaacaatcag
    13861 tgaagccata gaacaaaaat actgattaaa atgataaccg tccattttta taaaaaatgg
    13921 aacaggtgct tcagataaaa gtggcaagag cgagatggaa aattatcttg ctcttgttgc
    13981 cattatctta aaagcagttt gcggtttagc aagtttagag ggggcattca gatgcagcaa
    14041 gataaaacaa gcaatatcaa cgaaaaaaaa atcacgcttt gttcacttat tccagggatg
    14101 gggcagtttg taaatggcca agcttttaaa ggtatcgttt ttttcgccat attcgtccta
    14161 ttcattggtc agatgcttct gggcggattt catgcattgg taggattggt tactttggga
    14221 agtataccaa tggaagatca ttcattattt attttgattg aaggcacttt gcaattcatc
    14281 attacaatca ttttcttgat tttttacggt ttgaatcttt tagacgctta tcgagtagcg
    14341 aagatgcgtc aagagggcaa aaaaatcaat cggacgatga aggaagtcat acataatata
    14401 ggtgatgaag gttttcctta tctattgaca cttcccgctt atcttctcat gatttttacg
    14461 atcattttcc cagtcctagt tacattgttc actgcattta ctaattatga cttcaatcat
    14521 attccaccag ccagtttaat tgattgggta ggggctaaaa acttttttag tatcttcttt
    14581 ttaagctcat atcgtaatac tttttttgca gtcttttcgt ggaccgtgat ttggacttta
    14641 tgtgcaacaa ctttgcaaat cacccttggt attctcacag cggttgttgc tcaccagaaa
    14701 tttattaaat tcaaacgtat tttcggtatc attttcttat taccatgggc ggtcccagct
    14761 tttattacta tcatgagttt ttcgaatata ttcaacgata gcgtaggtgc catcaatact
    14821 caagtgattc ctttcataaa ccagcttcct tttgtagata tcagtgcaat ctcttggaaa
    14881 acggacccga actggacgaa agtagcaatt atcttagtgc agggctggtt aggatttccc
    14941 tatatctatg tgatggttac aggaatctta caatcgattt cagaagactt atatgaagca
    15001 gcaaaaatcg acggagcaaa tgcatggcag cgcttttcaa acatcacgct tcctgctgtc
    15061 ttcctagtgg cagcgccaac atttattaca cagtatacgg ggaatttcaa taatttctcc
    15121 atgatctatc tgtttaacaa tggcggtccc ggaagcgtag gcggaggagc tggttctact
    15181 gatattctta tttcctggat ctataaactt acgactggaa cttctccgca gtattctatg
    15241 gcttcagcaa tcacgttgat tatctccgtc atcgttatct gtgtgtcatt gctggtattt
    15301 aaaaagacaa atgcgttcaa tatgggggat taacaatgaa aaaatttcat tcgagtgctc
    15361 gattccaaaa acgactttct cactttttta cttacttttt tcttttaata ctgtcagtca
    15421 ttatcatcta tccgcttttg atcacagcaa gtactgcctt caagtcagga aatattgcag
    15481 cttttagttt agatctttca agtgactgga ctttaggaaa ctttactcgg ctattcagtg
    15541 aaacacttta cgggacttgg tataaaaata cattgatcat ttctgtccta acaatgatcg
    15601 tacaagtgac tgtagtaact ttggcgggct atacttatag tcgctatcgt tttatcggcc
    15661 gcaaatcaag tttgatgttc tttttagtga ttcagatggt accgactatg gcagcattga
    15721 ctgcctttta tgtcatggct ttcttattag aagcgctcga taagtactgg ttcttgacaa
    15781 tgatctatat aggcggtgga attccaatga atacttggct gatgaaagga tactttgaca
    15841 ctgtaccaat agatttagat gaatctgcta aacttgatgg ggcagggcat ttcaggattt
    15901 ttttccagat tgtattaccg cttgtcaaac cgatgctcgc cgttcaagca ttatgggctt
    15961 ttatgggacc ttttggggat ttcatgttag ctaagttcct gcttcgaaca cctgaaaatc
    16021 agactgttgc tgtcggctta caaacattta tttctgatgt gacaaaccaa agagtctctc
    16081 tttttgctgc tggggcaatt ttgattgcac taccgatttg tctattattc ttctttttac
    16141 aaaagaactt tgttaccggc ctgacatctg gcggaacaaa aggataggag cagagtatca
    16201 tgcaaacatt tccgttaaac tatttttccg cattacgtac gccaactcaa gtgtttgcgg
    16261 gacgcaaact attatcatgg cctaaatttt tcttgatatt tgtattttta gtttccttga
    16321 tggtcatgcc tgtgactctt ttttatgcca atcagatcca agcgattcca ttagaacaat
    16381 ttctatccgt tcattcgctg atcgatgaac aaggaaccca aaaattttct gaacttgaat
    16441 tatctgaaac tgggttacaa gcatcgcaac aaacgatagc tgtgacccct gaaattttgg
    16501 taggtgtgtc tttatctgag aaacaacaat ccgatcatgg aacttttatt gattttgaga
    16561 aggaacaatg ggtgatccaa caaaaggata aatctggtat acgccgctat acgatgaatt
    16621 atagcccatc tttccaacca gattctgtac gtacgcccga agatttccaa cgatttttag
    16681 aaagagaatt ttatgccagt aatcgtccaa caatcatttt atcttatagt ttaagtcttg
    16741 gtcttgtcct atttgtcatg acgagtctca ttttattcgg ggcttccttt ttcttgtgga
    16801 tgacaagaaa aagccaactt tcatccattc atacattcaa agagagtgca aatttgatgc
    16861 tgaatgttat gggaataggt tccatggcag cagcagttgt agggtttatc cactttgact
    16921 ttatcttgat gctaagtgtc caaacaacga taactgtctt gctgctttta tgggtttttg
    16981 tcaaaacaag attcaaagat aagcgtgtcg aatgacaagt ttaagcgatt tatgcgacaa
    17041 tgaatgtgaa aaaaatgaaa aataaagtag tttttgaaaa atttctgaaa aaattaaact
    17101 tcttagttga ttcttttctt ttttagtatt aacgtaatag ggtagttttt ttaaggagga
    17161 aaatcatgtt agagaaaact ttatacaatc aattattttc acgttctttc tcgctccctg
    17221 tcgaagtaaa ttattgggac ggtacaacga aacaatacgg agatacggac actcctcccc
    17281 aaatcaaaat caatatccat gaagaaatcc caatgaaaga gattacaagt aacgcttctt
    17341 tggcactagg agaagcctat atggatcatc gaatcgagat cgagggaagt atccaagcat
    17401 tgatcaacga tgcttatcag caagcaaaca gctttttacg aggaaaagat tatctaaaat
    17461 ggctgccaaa gaaagaaaaa catacgaaac aaaaaaacaa agaagatatc catgcgcatt
    17521 atgacttagg taacgacttt tataaacttt ggctagatcc aactttgact tattcttgtg
    17581 cttatttcaa tacacctgaa gatacgttgg aacaagcaca aatcaataaa gtccatcata
    17641 tcttagacaa actctttatc aaagaaggcg aaacgttgct tgatatcggt tgtggctggg
    17701 gaacattgat gttcactgct accaaagagt ataatgtcaa ggcaactggg atcacactaa
    17761 gtgaagaaca atacgatttg attaccaaaa gaataaaaga agaacattta gaagataaat
    17821 gccgtgtatt attaatggat taccgcgaac taaaaggcga aacgtttgat catatcacaa
    17881 gtgtaggtat gttcgaacat gtaggttctg aaaatcttga aggttatttc aaagtggtaa
    17941 aagatttatt gaaaccaaaa ggaacagctt tgatccatgg aatcagccga caacaaggcg
    18001 gtgcgacaaa tgcttggatc aataaatata ttttccctgg cggttatgtc cctggtgtta
    18061 ctgaattagt tgataatatt acaaaaaacg atttgcaact gattgatttg gaaagcttac
    18121 gtcgtgacta ccaattgaca ttagaacatt ggacaaagaa ttttcatcaa gtccaagatc
    18181 aagtcacgaa agaaatgggt gattccttct atcgaatgtg ggatctatat ttacaagctt
    18241 gtgcagcttc atttgaatca agtaatatcg atgtgattca atatttgctt gttcaacctg
    18301 gaaacaatga catcccaatg catcgtgcat aattgaggca aaagtactgt ttaaagacta
    18361 tttaaaaaga agctaagttt tatgatgctc cagaagtgaa cttttttgtc taacttctag
    18421 gattgtctct aaagcttagt ttctttttct ttatattttc ataataaaag tttggttctt
    18481 taatttttaa acatgacaga aaattttttc ttcgttatac ttgtaataga atactatttt
    18541 ataacaaagg ggatcgaatc aatgaaaaaa actgttattt atactagttt atttgcagct
    18601 ttgtttttag ctgcttgtca atcaaatacc aatcaaggca catctgggaa tacgtcttcc
    18661 agtagcgaac agcgctcttc acaggtatcg gactcctcaa caaaagcaag cacatcttcg
    18721 tctagccaga caaaaagttc tagtgaagca agtactcagt cagatatcaa agtgtctgca
    18781 gctcgtgcga tcgaattgtt ccaagataaa tacccagata ctgcaattac tagtttggaa
    18841 ttagatagtg attggggatc ttacttctac aagatagaag gtgtcgatga tcaaaaagaa
    18901 tacgaagcaa gaattgatgc tcaaaatgaa aaaatggaga cagagaatcc agaaactcta
    18961 gatcgagatg aacaaaatgg tcaaaagaaa aaagaagatg gtctggatgt tgataaattg
    19021 atcacgattg aggaagcagc aaaaatcgcc gtcgacaaag taggaagcgg tacggcaaca
    19081 gattgggatt tggataaaga tttaggtact acttattggg aagtcaaagt aaaagacggc
    19141 aatcaaagta catcagtcaa gatcaatagc caaacaggcg aagtactgtc aactgaacaa
    19201 gatgattaag aatttcttcg gaaaacaaga gttgattttt cttttagtca tgatacacta
    19261 gacaaaataa cttgtttgaa ggagcgattt acttgagttt agaaacgacc tttgccaatc
    19321 atatctttgc caatcctttg atgaatgcat ctggtgtcca ctgcatgact acccaggaat
    19381 tagatgaact ggctcattca gaagcaggag catttatcac taagagctgt acgatcaatg
    19441 aacgaaaagg aaatcccgaa ccacgctatt ttgatgttcc tttagggagt atcaattcta
    19501 tgggacttcc aaacttaggt ttttcttatt atctagagta tgctcttgcg tatgaaaaag
    19561 tgcaggaaaa ccaaaatcaa ccgttatttt tttccattgc cgggatgagt gtccaggaaa
    19621 acttggaaat gcttgagaaa atagaaaaaa gtgggtttaa tgggatcact gaattaaatt
    19681 tatcttgtcc caatgtgcct ggaaaaccac aactagctta cgattttgag gccacttatg
    19741 aaacactgaa agaagtattc agcatctttt ctaagccgct aggaatcaaa ttgcctcctt
    19801 attttgattt tgctcatttt gatcagatgg ctgatatctt gaatcagttt ccattgacct
    19861 atgtcaatgc tatcaacagt gtgggcaatg gtttgtatat cgatacagaa caagaagcag
    19921 tagtcatcaa accaaaagaa gggttcggcg gcattggcgg agagtatatc aagccgactg
    19981 ctttggcgaa tgttcgcgct ttttatacac gtttgaaacc agaaatccaa atcattggta
    20041 caggtgggat tcggacagga caagatgcat ttgaacatct gctatgtggc gcaagcatgt
    20101 tacagattgg taccgagctt cataaagaag ggccggaaat tttttctaga atcataaaag
    20161 aattaacgca gattatgtca gaaaaaggat atacgtcaat tgacgaattt aaaggcaaat
    20221 tacgcaccat ttcatagcaa agagacgaag gtcatgattg atcttcgtct ttttttataa
    20281 aaaacactcg tctgttcatt cctgtgcaag cgtactcatt gtatgttaga cgaacttgtg
    20341 ggaaaataac agaggcaaaa aggaggataa tatgaatgat gcaacagaat tagcaaaaga
    20401 gcttaaagaa aaagaattta gttttcaaga actagttacg gatatacaga aaaaaataaa
    20461 gaagcaaaat catgaattga atgcgtttgt cgcgcttgaa ccattcgaga gtaattccca
    20521 cgagctattg attccccagt cagaaaaagg gccattggct ggtatcccat ttcctttaaa
    20581 aatgctcggt caagaaaaaa agggatggct agctacttca ggatctcgtt tatttgaaac
    20641 acaccgagct tctcatacat ctaattatgt gcggcaagca gaagcgatag gtctagttcc
    20701 ttttggacaa accaatgcgc cagagtttgg tttcaaaaat attacagacc cagtcatcta
    20761 tggcccagca agaaaccctt ggaaccttga atgtacaccg ggcggatcaa gtggaggagc
    20821 tgctgctgca gtttcatcag gaatcgttcc tttagctggt gctagtgatg gcggtggttc
    20881 tattagaata cccgcttcgt tttgcggatt gattgggctg aaaccttcgc gagggacgat
    20941 gccagttggt ccatatgctt ggagaggatg gcaaggagct gctattgatt tcggcttgac
    21001 agtttctatg agagacacgg aggctttatt ttatgggatg agaagtatcc attccggtgc
    21061 gccatatcaa gtatcgccag ttgagtggca aacacatcca agaaaaaaac ggttgaagat
    21121 agctttttgt gttgattcac cgatccactc acagattagt gaagaggcag tacttgctgt
    21181 aaaaaaagct gtagatttcc ttggaaaaga agggcatgat ttgacagaga ttccttaccc
    21241 actagatggt cgtaaactga tcacgtctta ttatcagatg aatggcgcag aaacagcagc
    21301 aatgatccgc tcgattgaaa caggcatcca gcgagctgtc agaaaagaag aacttgaacc
    21361 catttcttgg actttgttcc aatatggtca gaagctctct gccgctactt acatcgattc
    21421 tctgcatgta tgggatgaag cagcaattgt catggaaaac ctacatcaag aatacgatct
    21481 attcctttca ccaacgactg catttaccgc ccctaaaatc agcgaagatt tgcaaagtga
    21541 tgcgataaga gcacagatgg aacacatcag tgattgttct gaagtagaag gtttagacgt
    21601 tgtttcttcc atgtttgagg agagcttgaa gatcacacct tatacacagt tggctaatct
    21661 gactggacaa ccagcaatca gcttgcctac tcatttgaca aaagaaaaca tgcctctagg
    21721 aatccaattt atggctgcca gaggaagaga agatttgcta tttcaaatcg gctatctatt
    21781 tgaagcaaat catcagttcc atttaccaaa tccatggaaa tagcagaaac aaggagaaga
    21841 agaatgaaga aaaaaagatt gtttgctcta ataactagtg ccttttttat tttaagcgct
    21901 tgcggtacag ctgccgctaa tcaggaagat aacagctgga gtaatgtaga aaaaaagaaa
    21961 gaattagtag tagcgacttc tggcacactg tttcccgcat cttattataa tgatcaaaat
    22021 gaattagttg gttatgatgt agatttggca aaagaggtgg ctaaacggtt aaatgtcaaa
    22081 atctccttta aagaatacaa cgtggatgga caaatcactt ctgtttcaaa aagagaatca
    22141 gattttgctg cgaacgattt tggtatcaca aaagaaagag aaaaaaaatt ctccctttca
    22201 gagactttca aatattcatt tgacagtatg atcgtacgaa aaaaagatga ttcaggaatc
    22261 cactcacttg aagatcttaa agggaaaaaa gcagctggcg aacccaatac tagctatatg
    22321 cgtttagcgg aaaaacacgg agctaaatta gtcacatatg acaatgccac gaatgatcag
    22381 tatttaagcg atgtagcaaa tggtcgaaca gatgttattc taaatgatta ttatctgcag
    22441 aaaatggccg tatctgcttt accagatatc cctgtaaaaa tactggagaa cgtttatttc
    22501 aatgaaaacg aaagtggttt tttattgaaa gaaggaaatg atgctctgaa aaagaaaatc
    22561 gatggcgtat taaaagagat gaaagaagat gggacgacaa agaaaatatc tgaaaaatat
    22621 tttcaaactg atgtttcagt caaaccgaaa gaaacgatgg ctgagtcggt gagtgagtga
    22681 tgtactttcc tctaattgat ttttccctca tgtggacttc acttcctttc gtactgcaag
    22741 gcttggtata cacattaggg attggcttcg tgtcgtttct tttaggaaat ctggtagggt
    22801 tgcttttgac agttttaggt cttttggatt ggctgcctct gaatgtattt attcgttttt
    22861 atttatcgtt ctttagaggc attcccgcac tagtcctgct gtttctgtta tattttgggt
    22921 taccttatca attaagtgcc ttaacggctt cagtcatctg ctttacgatt accagcagtg
    22981 catttattgg tgaaatctat cgaggatcac tagcaggcgt ttcttcaggt cagtgggacg
    23041 ctggtttagc ttctggtttt tctttctttg gggtattgaa atatatcgtt ttgccgcaag
    23101 cttttcgaat ctcagtaccg gcactcagta acgtagctat ggatctagtg aaaggtactt
    23161 cgttagcagc aacaatcaca gtgccagaaa tatttcaaaa agcaaagatc ataggcggtc
    23221 ggacattcga ttatttttct ctctatattt tagttgcact gatttattgg gtcatttgta
    23281 tcgggatcga aaaagtgcaa cagtatttag aaaaacgttc tatgcatcag tcaatctgaa
    23341 aaatagaaat ggtgctagcg tggctggcac catttctatt ttttgtttct tgtatattct
    23401 gcctagtatt ttaaaaccat ttcatgtaaa atgtaaatcg aaaatatgtt cggaggtaga
    23461 aaacatgaag accccgctaa ccatcgaaat agaatcctcg ttataccaat attgcatcga
    23521 gcaaggcggc ttggttgtag aagaagtcac gatgcctgat gaccaaggaa ttgtagatac
    23581 gcttagctgc ttgacaaaat ttgatggaag ccgagaatgg cgatgctatg agctgaaagt
    23641 gacaaaagca gattttcgct ctacagccaa actgtcattt gttggacatt ataattattt
    23701 tgttttacca agtaaactat atgaagaagt aaaaactgaa atccctacag gaataggtgt
    23761 tttagtgtat cgtccgtaca cggttgaaga ggagatgcct gcctctggta catttctgat
    23821 tgcgaaaaaa gcagcaaaga aagagcttgc tgtaccggaa gaagctttga caaaccgctt
    23881 catggcttct ttattcaggg aagtaagaaa agcaaagcaa atggagcgag gcgtaaaata
    23941 ttttccaaca gatcaattgt acaaagaatt gaaaagacgt tcagaaaatc aagatccatt
    24001 ttctaacgaa cgatattatg aacggttttt agaggaaata gctagtcagg caatcaatga
    24061 gttgcaagaa gaagttatcg cgcttcagca tgattatgaa tatttgaggc aacaacagca
    24121 gatccgtcgg ttgcctacag agccattgga gtgaaaaata tgtactatcc ttatttaaga
    24181 ggaaaacaat ttgacctatt agcattaaaa gaagcactgt cgcgaggatt gctctccaat
    24241 aaaatccaac cagtgatcga accagtacgt gattccgcta cgcttaaaaa tgtgatcgaa
    24301 ttatttcaaa aaaagcacca tccaatcgct gtgatccaaa atccccaagt aggacaattc
    24361 aaattattcg atcagcatgt gcacagttgg acagtcaaag aaaatagctc tgtcgtccct
    24421 gcacagatct taacgccaga aaatctcagt gaagtactgg attcacctcc agcgtttctt
    24481 gttttcgatg gacagcacta cccaaaagat acagaggtgt ggaaacagtt ggcaggagtt
    24541 gattcgaaat ttttgatacc agatactagt cgctttcgca tctggctgcc cgaaaacaaa
    24601 attgtgatcc gtgactcttt tcaaacgaga aagcatgttg aaagttatgc agataaaaca
    24661 gatgactttt ttagtgatga ctatcttttt ttccacgcag acggatatat tggattttct
    24721 gatttcacga tcgaaggtag ccgttatttc gataaagggt tccctagccg agcgcttgct
    24781 atccatctaa cgtatattga tgcttacggc aatatacgcg tgaaacattt tgtctctgac
    24841 agtaacgact ctgctaaaga ccaagcattg aagtttcttg aagcaggaga taaaatgaaa
    24901 aaatggatca tgcgtcacca tcatcaactc ttgattactt caggaatgat tgaattattg
    24961 gctctttacc agcaacaaaa attccctgga ctgggagtgt tgaaaaagtg gtcacttatg
    25021 catcatttgg aacttattag tcacctgcta gatcatccaa aagattggct taaatattat
    25081 tcaaaagttc aggaactata tgaagtgatc caatgattag ataatgtaaa tgaaaaagga
    25141 atagaaaaga ggaaactgga tgtatattcg gcaaacaagg aaagaagaag tagaagaagt
    25201 ggtagaaatc attaatgccg cagcggattt cattgcttcg caaggctctc ctcaatggca
    25261 aaacgggcag aaaccaacaa aagaaacgat tttgaacgat attgaaagaa aagaaagcta
    25321 tgtattaata gtggaagatc aaattgctgg aacagctgca ttagtctcaa gcgtggatcc
    25381 cgtttacaca aaaatagatg gtagttggaa agaatcaact ggtccttatt tatcgattca
    25441 tcgtgtcgct ttagcagaaa gtactcgagg aaaaaaacta gcaaaaccgt tcttagaaca
    25501 tttgttgacg attgcagcca gcaaaaatat ccaagatatc cgtatcgata cttatccagc
    25561 taatgaaccg atggaaaaga ccatctattc tgctggattc acttatcaag ggatgattga
    25621 atttcctttt gaacacggcg aaagaaaagc atatcaaata atcatcaatt gactataaaa
    25681 tcatctgagc aaagataaag acattaacac tactgaaaaa aacaatcata tgatagccaa
    25741 gaggttgtaa cagaagtggc cagcttcaaa aaacaagaaa gaatttccga aaatgcttct
    25801 caaatttttg tggaatttta gcgtatttta cgaaatagct gctttctgaa actcccttca
    25861 tcgagaggtc gtgacatagc ttttgtcacg acctctcggc ttatcctacg agtattattc
    25921 gatacattta tatcaataat ccttttttta ttttatctgc agcttcttgg aacgctccat
    25981 cgtttgacac gactaaaagt gaggggatag ctttcaaacg tttcaactgt gctaaatctt
    26041 ctacataaac cgccagtctg gctgaaatct gttcagatga atctccacgt tttttcaggc
    26101 gcgattgcaa tgtttcttta gagatcgtaa taaagatagg acggactacc gtcccgaaat
    26161 actcatataa atgccaaaaa ccatttgctg tcaaaacggc gtaacaattt cctactgtca
    26221 gtttcttttt aatttctgct ttactggaac catatttatg ttgagcatat tggtcccact
    26281 ctgcaaactc atttcccttg atcatctgat tgaatgtatt gtcagaaaca aaataataat
    26341 cctcttgatc tttttcccca ggtcttggtg atcgattcgt gtaagtaatg atcttttgtt
    26401 ccggctgaaa aatagcttct gcgagtgaag tttttccaga accagaagga ccgataaata
    26461 catagcaata attcattgtc atttctcctt tgatgattga acaagtgaaa aagctttgga
    26521 aaatatttcc aaagctttta aaagtaactt tgcttttttg tatattatac cagcaagcca
    26581 gtcacaatag cacttaaaaa gctgactaaa gtcgctgtta gcaaaatttt caatccgtgt
    26641 tttgcgacaa gatttccttt ttcttcattt aatcccttca tcgcaccaga aatgatcccg
    26701 atagaagaga agttggcaaa tgaaacgagg aagacagata taattgctgt tgtacgctca
    26761 gtaaaatgga aattaccatt tgctaattct gtcatagcaa caaattcatt tgtaaccagt
    26821 ttagtcgcca tgatactacc ggcatccact gcttctttcc atgggattcc gctgatgaag
    26881 gcaagagggg caaagatgta acctaggatc tcttggaaag tgatgccgaa tatcccgtgg
    26941 aagatagcat tgatcatggc aatcaaagca acaaaaccga taagcatagc agcaaccgtg
    27001 atcgccacat ggaaaccatc aagaatgtat tctcccaaca tttggaaaaa tgtctgttct
    27061 ttgttttctt caatgatcag ctcatcttct ttttcttcta atacataggg atttacaatt
    27121 gaagccagaa tataaccgcc aaataagttc aaaacaaggg cagtgatcac atatttagaa
    27181 tcgatcagtg ccatataaga tccgacgatc gacatagaaa ctgtagacat agccgataca
    27241 ctcatggtga acaaacgttt ctcagatagg aagggtaatt ccttttttat ggaaataaaa
    27301 acttccgatt gtcctaaaat agcagaagct actccattat aagattccaa tttgcccatc
    27361 ccgttgattt tacttaatcc tagaccaaca gctttcataa ataatggaag aatcttgata
    27421 tagcgtagaa taccgataat agcagaaata aatacaattg gcatcaatac ggaaagaaag
    27481 aaaggtgcac ttcctttatt tgctacaccg gcaaaaacaa agtcaacacc ttgcccagca
    27541 aaagcaagaa gatgatcaaa catttttgcc aatacagaaa caaccgctgt accaaaagtt
    27601 gtacgtaata atacgaaacc aagaacaagc tgtaaaacga acatcacaat tagtggtttc
    27661 aatttgattg cttttttgtt actgctgatc aaaaaagcaa gccctaatgt gacaaataaa
    27721 ccaataatac ctaataaata actcaaataa tcactcctat tccaaattcc gttttatttt
    27781 acctgttttg caaataaaat actaatcttt agtgaaattt tcattacttt tttagagtga
    27841 aacgcattca ttttccatta gaaaataaag cgaacaaaat aaaaggttta aaaagtgtaa
    27901 cgtcttagta aagtctataa aaaaacgaaa ctatcgatta agctaatagt ttcgttttca
    27961 acatttataa gtaaactttt tctattggta atatagattt tcggaagggt agacaggtgc
    28021 gcaggtaata tcgattccta gttttttcaa tgtttgttca tcatttttat tgaccatcac
    28081 agtagagtgg gcttgtgctc cctcaagttc agctagttta tcataagcaa cttgagcgat
    28141 tggattcgtt actgcactga ttgataaagc gatcaagact tcattgatgc ttagtgcatc
    28201 caaagaggaa tgaagtgtct ttgatttcaa tccttgtatt gtttcaagaa ctaatggtga
    28261 caataaagga atgtcatctg cgatatgggc taaatgtttt actgcattta agataactgc
    28321 cgctgacgca tccatcaatg aacttgtacg cccggtaatg atgacttcat ctggtaattc
    28381 gattgcaatc acagcagaag gatcttttgt aggactttgc gcttgaatct tatctgcata
    28441 gtcatgggca gggactacca cgtttcggtc ttcaggacgt aaaccgactt cttccataat
    28501 cagtttgatt cgattaactg cttcttcgtc gatcaatcct tttttgaagt cgcattctgt
    28561 ttggaaataa cgacggatga tttcttgttt agaggcttct tggacaacgg tatcatccac
    28621 gatcccaaat cctacacgat tcacacccat gtcagtagga gattgataaa cagattcttt
    28681 accagtaatt ttttcaataa tacgtttgat tacaggaaat gtctcaacat cccgattata
    28741 gttcacagct actttattgt aggcatcaaa gtggaaggaa tcgatcatat tgacatcttt
    28801 tagatcgact gtggctgctt cataagcaat gttcagcgga tgtttcaacg gaacattcca
    28861 tactgggaaa gtttcgaact tggagtaacc agctgcatgt cctttttggc tttcatgata
    28921 cagttggttc aaggaagtag cgagctttcc acttcctggg ccaggagctg tcactacaac
    28981 gattggttta gttgtttcaa tatactcatt ttttccaaaa ccatcttctg agacgatttt
    29041 ttctacgttg actggatagt cttcgatttc tgagtgttta tagaccttga tgtttcgtct
    29101 ttctagttta ttgataaaga ctttcgtggc aggctgtcca ctatagcgag tgataacgac
    29161 actgttgatt aataagccat aaccacgtaa ttcatcgatt aaccgcaaga tatccatatc
    29221 ataagtaatc ccataatcac cacggatctt attacgttca atatctccag catagacgca
    29281 aatcagtatt tcagcttgat ctttcaattt ttgcagcaat ttgattttcg catcttcttc
    29341 aaatcctgga agtacacgtt tcgcatgctt gtcgtcaact agcttcccac caaattctaa
    29401 atataatttg tcatagtggt tcactcgttc caaaatatac gctgactgct cttcaatata
    29461 tttttctgaa tcaaaaccaa tttttttcac taggttccct ccttaagtac taaagcattt
    29521 tttcattttt ttgaggattc gtcaactgga agagcgaaat tcctcaaaaa aacaaagaaa
    29581 atggtttaaa ccaacttgtc tagatagtta ttcattggta tgatagtttt tgataaggaa
    29641 ggtgctacat gaaaaaaaga cagaaaaaga aaaacgcata taaacactat atccgttcga
    29701 tttttaccgg ttatgaaaga atgcttgaag atcccgaatt ggaacaattg acgtttactt
    29761 atctaaacga agaaacgcag ctaactcgtg atgagcatca gcgcattcat ttcacaacaa
    29821 gagatctgcc tagtaaataa aaaataagat aaaggtcatc ggattcgatt ctgtcctatc
    29881 agatggaaag actagttgat cgaaaaaaag gctgtaccaa attttggtgc agcctgtttt
    29941 atggagcatt gcccgctctt ttttataaca attttttaca gaaaatggta ctattgaagt
    30001 attaataagt gaaaaacaga tgagtaagag gaggcaagta gtatgaaaga gccagataca
    30061 aatgcaaatt atgagccaga cgaactagag gagacgaatg cggatcagga tacaggacaa
    30121 gaatcgtctt ctttttacga tatcaaagaa aaagcaagca tgaatctttc ccaattatta
    30181 gaagacttac aggcttttga aaaagctgtc cgcgaagaag atatccctga gatctaccgt
    30241 ttatacaatg gccgattgaa tgaagaatta aaaagaagct caaatgaaaa tcacgagatc
    30301 gatcaattgc tgatgcgtaa gatccatgat cgttttttac aggaatttcc ttttatggaa
    30361 caagtagaaa acatctcgcc aacgatgagc tattacaaaa tcggcactta ttaccatgat
    30421 cggcccacgg tcggtattga tgcaagtctg ccagaaattt ttgttttgcc taagatcgat
    30481 gaagagtggg agaaatattc ccaaccggag tcaacggact atgacaaaaa aatcaatgag
    30541 ctagacgcaa aggtgatcac tgctcagaca gaaattgaac gtttagatga acaaataaaa
    30601 gaaatcaatc gacaaatgac tgatttgaac gacaataaag gattcttgaa tcgtaaaaaa
    30661 atcgaggaag aaattcaaga attagagaag aagaagcaag tactatctaa tgaaaaatta
    30721 ggctggcttc cttatattga gacgcctgaa acaatccagc agcaaaaaga agcattgaag
    30781 caagaagcaa gagcagacca gcttcgtgca gccatcgtag aaaaagaaca aaggcaaatc
    30841 agacgctatt tcggtagcaa agagggattc ggtcaagcaa tccatgaatt cttgatgaat
    30901 tatcttggca atgagaatcc taataatcaa tctaatgaag gaggttccga gtatgaatga
    30961 acacgatgaa gcagaatgga caaaaaatga agaacaaaca gaagatcatg aagaaacgaa
    31021 cacttcattg gaagaacaaa caagtaatcc tctggatgac acagaagaaa tccctatgct
    31081 agaggaaaca aaaatacacg aaaaagaaaa agtaactccg acgaatacga atactgcaga
    31141 aaacacccat gcttacgaaa taactgaaaa tcccgggtat ttagaaggaa aagagcaaga
    31201 tcatcaagat atcgtcagtt tgattgaaac agcggaagca gaattagcgg atttacgtat
    31261 gagaaaagat ttgatcgaat cagattttcc acaattatta gaatcatatg atcgttttat
    31321 attgaatgaa gaaccaatct ccacgatcgg aaagacaagc ctgatcgaat acttcacgta
    31381 tgaattactg aaaccgttga atgatattgg tctggaacgg tcaaattcag cttatgacac
    31441 atggaaaaca agacattttt cgatcaatta tacgttgaat ggacaaaacg aattagtctt
    31501 ttcctttgta ttaccgacca tcgatcaacg ataccaagtg gaatcgatga gtctcctaaa
    31561 cgtatcacca gagacaatgg aaatcaatgt ccaagatgac cgggtgcttt ctttgatccg
    31621 acactggtca gtggatcgag tattctctgc agggcagatc acgatattca atcacaaatt
    31681 gaatcaatta ttagcccatg caagaacact tggttttacc gttaatcaga cattactaga
    31741 caatacgaag ccacttcgtt tgaatcttca gagcgaattt gaattgacga atgaagtatt
    31801 ggatgatatt tttatcgtgg caatgaaaaa tccatcctac gatatggaaa agatcaacga
    31861 aactacttat caagtacgat tagataaagg acagtcttta acgataacaa caaacgaaaa
    31921 agatcaaaca gtcttgtcga tctcatctgg agactaccca cgatctgtca ttgatttctt
    31981 tatcaattac gaattcttgg tcccattaat ggtgagaaag tcctgattgg ataagttgga
    32041 ttaccagatg tcattcagat aattctgttc caaaggactt gcggcagtta tccagaataa
    32101 ttgtcacaag tcttttctgt atgtactaaa tggacgtgaa taacttgctg atgaaggagg
    32161 gatttcatta ggaatgtcca caaataaaat agcacagctt agcttattgt ctgctgcttg
    32221 tgtagcaggt aggatcgctt ttcaatttat tccgaatttc cagccaatga ctgctatttt
    32281 tttatttata gtactttact taagcttaaa agatgcgtta attgtcatga gtctttctat
    32341 tgcgatcagc agtttttatt ttggtgttgg accatgggtc atcggccaat ggatcagcta
    32401 tactgttgtt ctgagtttgt tttcaatcgt ctgcttgaga aaaacagtgc aaaggaatct
    32461 atggttaaag ggagtctgtt tttttctggc gggcatggtt tacggaatag gtatgaccgt
    32521 ttttgatacg ctgttatatc gtttgcctca accttggatc tattaccttc aaggggtgtc
    32581 gtttgatttg atgcacagta taggcaatgt tgtgttcttt cttgtttttc tgccagtcgt
    32641 caaacgtttt tacaatcatt caggaggaaa aaatgaaaaa aataatttat agtttgcttg
    32701 caatgagtgt cagtatactc attttgacaa gttgcagtac aacctataca caatcggctt
    32761 ccaaccaaaa ggaggaagta aaaacatcgc aaaaagcaac aatcacgtta caagaaaatg
    32821 gtcaaaactt ttcggaaaaa gaagtgttct tcaaaaaagg cgatgattta ctgagtattt
    32881 tgaaacgaaa ttttgaagtc aaagaagata acggcttcat cacttcgctt gaagggcact
    32941 cacaggatga gaaaaatcaa ctctattgga tgtttactgt ggacgggaaa agtgctacta
    33001 ctggtgccaa agagatcaaa ttgaatgatg gacaatcggt cattttcaat ttgtctaaac
    33061 tataagtttc ataaaaaact gttaaagggc gaatattgcc ataattcagt gagttcagtt
    33121 gatgaaagaa ggaaaataac atggaaaatt ttaaaaaaag cggaatgttt tccgtaattg
    33181 ctgctctagg ggctaatatt ctagttgcta tcagcaaatt tgtcggctat gcaatcagtg
    33241 gcagtgccgc catgctaaac gaaagcatcc acagtatcgt tgattgcagc aatcagattt
    33301 tgctgttatt cggtgataaa caagccaata aaggacagag cgagttgcat caatttggtg
    33361 aaggacgagc aaaatacttt ttcagtacaa tcgtcgcaat gatgcttttc ttcggcggtg
    33421 gggcacttgg ggtgatggaa gcttttgaaa agttgacgca tccatcccat gaagtgggta
    33481 atccgatcat tgttatagca atactgttat ttggtttggt aatcgaagga agttcgttac
    33541 gcgtggcaat gaaagaaatc aaagaattga atactgaaaa actttccacg atgcgctttt
    33601 tgcgcgaaag tcgacacagt gaaatactga ttatttttac ggaagacttg tgtgctgtaa
    33661 tcggtttggt cttggcttta gcaggaacaa tattgactta tgcaaccggg attgctgcat
    33721 ttgatgcgat cagcggattg ctgatcggtt tcttgctgat gggagccgcc attttcctag
    33781 caaaagaatt ttatagtctg atcatcggtg aaagcgtgac agcaagcgat ttatcaaaaa
    33841 tcaagttagc ttttgaacgt cctgagatca gtcggctgat cgatataaaa acagttcatt
    33901 taagtccaac ggagatttta gttgcagcaa aagcagatat cgttggtaac aaagagaatg
    33961 aagcttactc ggtcatcaat gatattgaaa aaaatatgcg tcagtctctt cctgacaaga
    34021 agatgtatat atacattgaa acagataaat atgacccaaa ttattctcgt aaataaaacg
    34081 tttattcatg aattgaagca aaaatgagaa aaatataatc ttgtttttaa ataaaacggc
    34141 tacaatttag cgaatttatg ttacaatgag tccgctgttt atttgttgta attaaaggag
    34201 atattttaat gaacaaaaag aaaagtgcgc catggcttca agctttgcat gcagtgatgc
    34261 cactttgtat aagctatata ccagtaggat tagcttgcgg agtattgttg caaaaagttg
    34321 gatttgatcc tctcttatca ggactgttat cgattctggt cttttccggt ggcgcgcagt
    34381 ttttagttgc atctatgctt acgacgcaag catcatttgc aacgacatta ttgatggttt
    34441 tctttttaga attacgctat acattattag gagcaagtct tgccggtttt atgaaaaaag
    34501 aaaagcggac gtttttggct gtcttttctc aatcactgaa tgatgaaaac tatgcggtga
    34561 actacttaaa gttttcaact gacccttcct ggaataaaag aaaggcttta tatgtcaatt
    34621 ggttctcaat gactgcatgg gccggtagca atatgatcgg taattttttc ggctcggtga
    34681 ttcgagtaga tgctgacctt gtccattttg ctttgacggc catgttcatt ttcatgttta
    34741 tcatgcagat gaaaagcgca ctactgatct ttacaggttt attctcaggc gtattagggg
    34801 tcgtattcat ggtattattc caaaatactt tcggattgat tgcagcaaca gtcattgctt
    34861 catttgccgg atttatgctg gagaaggcat tcaaaaaaga aaaagctaag aaactaagag
    34921 accgcggtaa agatgtcaat gagccgctct tcaacttccc ggaacaggag gccaatcacc
    34981 atgactaaac tatatttgct acttttagtt gcttgtctgt tcatcgtggc atatatcccg
    35041 cgcttgttcc cgatgcttta ttttactcat cgaaaagtgc catcctggtt cagtgactgg
    35101 atgaaatatg taccggtggc attatttgcg gcgttagctt ttaaagatgt tttcattact
    35161 catgaacatc tggatattgc ttggaatatc aaaatagctg caatgattct agtagcaggc
    35221 gtggcttaca aaacacgttc gatggcgttg tcagttttga caggacttgc gtctgtcttt
    35281 ttgctgtcta tgctttaaac tgttttattt tattaaaaac agagattgcg acataagttg
    35341 ttcaaactga tctatccgaa ctgcatttgc aacagtcaat tctggatgca agtttattcg
    35401 tagggataaa aaagattagt gttggcttat gatgcaagct cttttttatt ttaggaatta
    35461 gagagggatt tgtccttcta tgattttttg ctataatagg gaagatgttt attttatgaa
    35521 agaatagttg agggaattta aatgaataaa aaattgatgc aaggaacatt ttggctaacc
    35581 tttgctaatc ttctatgtaa agttttaggg gtagtctatt tgattccctg gctaagtatg
    35641 atggggaaca atcaagatgg tatgcttgct acgacattgt ataatgttgg ctatcttcct
    35701 tatggattgt tcctgatgct tggtacagtc ggatttccaa atgcgattgc aaaaaaagta
    35761 gctgtagcca caaaagaagg agataacgct gcttgtcggc tgatttttag aagtacgatc
    35821 aatatcatgt ttgtcatcgg tatcgcctct gctgcactga tgtatttatt tgctccttta
    35881 ctagccggaa tcagtccgat tgctaacgtt aataatggga tactagctat cagaagttta
    35941 tgcccatctt tgattgctat cccaattctt agtgcaatga gaggatattt ccaaggaaaa
    36001 aatgatctgc ggccttatgg aacttctttg attatcgaac aggctgtgcg agtgatcgtt
    36061 atcttggctg gtacttatta cttgcgcgta ttgactgacg gaaccatatt agaagctgta
    36121 ttgatcagta cagtcgcttc tttcttcggt gggttagctg cgattatcca tatgtatatc
    36181 gtcggacgca gaaaagactt cttcgaactt aaagattttt tgatttccag ccgttatttc
    36241 gaaagagaaa accgatctgc ctctgtctct atcattcggg aaacactacc gtttattttt
    36301 gtcggctcag tcattactat cgttcaaatg atcgaccaag taacgatgaa accactgctt
    36361 cactttttcc gtccagagat cgcagatcag caactggaat tcttattcag ccgtgcttct
    36421 gtcaatccaa ataaattgac attgatcctg atctccatgg taggaacagt agctataagc
    36481 agcttgccaa tattaagcac gatgaagaaa aaagatcgga tacaaatcga aaaaacagta
    36541 ggagatagtt tttcgattgc gttattgatt cttcttcctt cattgactgg aatgtcttta
    36601 ttagctggac cactatacac attgtttttt ggctatgatc ctgaaagtgt cggatatttc
    36661 caaatggctt tgctagcttc tttgtttttc tctttattca cgatcctatc tacgatgctt
    36721 caatctttga atcaccatcg ttttgcgatc cgattaactg tagaagcaat tatcctgaaa
    36781 gtcatcttcc aagtaatcgg attaggatta gttggcggat acgggatgag cttatcaaat
    36841 ggcttagcat tcggtattgt ctttgttcgc ggctatcact ttatgtgcaa agaataccga
    36901 atagcacctt tagcaaagat cagcaatttt ttcttaaaaa ctttccgctc caccctgatt
    36961 atgcttgcag tatgttttgc cgttttcttt ttgctgaatc agcgattgtc aatggaatca
    37021 aaaagctatg caatgattta ttgtgtggct gtaggaagta ttggcggctt agtattttta
    37081 ttttctcaat tcggaaaaaa tgggtttcga atgttgaaaa atgttaaaca tcatagcaga
    37141 agcaaagaat aggttaacta atcgtttttt gctgtcactt gaaaaacggc aactcgtatg
    37201 attacgcttg tcgtttttct attttccaat tttagtttca tatctaatct tacttatgat
    37261 atagttataa ggtctggaaa aattctttga aacgaggaaa ttaaacacat gaaacttgca
    37321 gtagttacag atagttcggc atatctgccg gaacgtatca agaaccatcc cgacttattc
    37381 gttattccaa tccctttgat tatggatggg aaaatttata acgaaggaat cgatatcgaa
    37441 gcggatgaat attatggact tttaaaaaac agtaaagatt ttcctactac ttcacaacct
    37501 gtcgtagggg aagttttggg tttatatgag gaattgaaag agaaagggta tgacacgatt
    37561 ttaagtatcc atctttcttc agggatttct ggctttgtca acacattatt tgcgatgaaa
    37621 gatgacatca gcggagtaac ggtcattcct tatgactcta agatcactag tatgccgatg
    37681 ggacacatgg tagaagctgc cttagactta aatgatgaag gtcagtccct tgatgaaata
    37741 cttgcacata tcgatcgaat ccgagataat acctatgctt atctgattgt agatgatttg
    37801 aacaatttag tacgaggagg acgtttgaca aatggtgcag cgctgattgg cagcttatta
    37861 aaaatcaaac cgatcctgac attttctgaa gggaaaattg ttttattcga aaaaatccgt
    37921 tcaagtaaaa aagcatttgc tcgagctgaa gaaattattg gtagaagaaa taacgaaata
    37981 caaccgccag tcaaacttta cgtgatacat gcaaataatt tagaagtagc gattgcggag
    38041 aaagagaaat tgcagaaaaa atatccaaat gcagaaatcg aaattggtca tttcggacca
    38101 gtcattggta cacatttagg tgaaaaagcc atcggcattg ccatttcagc gcaataattc
    38161 cttttattca aaaaacatac caaaaat
//
LOCUS       DAIGOK010000009        37828 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.009, whole genome shotgun sequence.
ACCESSION   DAIGOK010000009 DAIGOK010000000
VERSION     DAIGOK010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.009"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(234..1178)
                     /locus_tag="NOA01_000348"
     CDS             complement(234..1178)
                     /locus_tag="NOA01_000348"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCI0631578.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1474..2418)
                     /locus_tag="NOA01_000349"
     CDS             complement(1474..2418)
                     /locus_tag="NOA01_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCI0631579.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2639..3853
                     /locus_tag="NOA01_000350"
     CDS             2639..3853
                     /locus_tag="NOA01_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCI0631580.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASIYGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            3875..5890
                     /locus_tag="NOA01_000351"
     CDS             3875..5890
                     /locus_tag="NOA01_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-rhamnosidase"
                     /protein_id="HCI0631581.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEETFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(5973..7346)
                     /locus_tag="NOA01_000352"
     CDS             complement(5973..7346)
                     /locus_tag="NOA01_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCI0631582.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7372..7656)
                     /locus_tag="NOA01_000353"
     CDS             complement(7372..7656)
                     /locus_tag="NOA01_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCI0631583.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7658..9673)
                     /locus_tag="NOA01_000354"
     CDS             complement(7658..9673)
                     /locus_tag="NOA01_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCI0631584.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(9852..10157)
                     /locus_tag="NOA01_000355"
     CDS             complement(9852..10157)
                     /locus_tag="NOA01_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HCI0631585.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10246..11277)
                     /locus_tag="NOA01_000356"
     CDS             complement(10246..11277)
                     /locus_tag="NOA01_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCI0631586.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11484..12359
                     /locus_tag="NOA01_000357"
     CDS             11484..12359
                     /locus_tag="NOA01_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCI0631587.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12511..13353)
                     /locus_tag="NOA01_000358"
     CDS             complement(12511..13353)
                     /locus_tag="NOA01_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCI0631588.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13445..15322)
                     /locus_tag="NOA01_000359"
     CDS             complement(13445..15322)
                     /locus_tag="NOA01_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCI0631589.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVCNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15319..16740)
                     /locus_tag="NOA01_000360"
     CDS             complement(15319..16740)
                     /locus_tag="NOA01_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCI0631590.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16743..20132)
                     /locus_tag="NOA01_000361"
     CDS             complement(16743..20132)
                     /locus_tag="NOA01_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCI0631591.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20389..21780
                     /locus_tag="NOA01_000362"
     CDS             20389..21780
                     /locus_tag="NOA01_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCI0631592.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(21853..22500)
                     /locus_tag="NOA01_000363"
     CDS             complement(21853..22500)
                     /locus_tag="NOA01_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCI0631593.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22508..23194)
                     /locus_tag="NOA01_000364"
     CDS             complement(22508..23194)
                     /locus_tag="NOA01_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0631594.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23200..24273)
                     /locus_tag="NOA01_000365"
     CDS             complement(23200..24273)
                     /locus_tag="NOA01_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI0631595.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24495..25169
                     /locus_tag="NOA01_000366"
     CDS             24495..25169
                     /locus_tag="NOA01_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCI0631596.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25174..26514
                     /locus_tag="NOA01_000367"
     CDS             25174..26514
                     /locus_tag="NOA01_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCI0631597.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26663..27649)
                     /locus_tag="NOA01_000368"
     CDS             complement(26663..27649)
                     /locus_tag="NOA01_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HCI0631598.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            28373..29404
                     /locus_tag="NOA01_000369"
     CDS             28373..29404
                     /locus_tag="NOA01_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCI0631599.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            29513..31045
                     /locus_tag="NOA01_000370"
     CDS             29513..31045
                     /locus_tag="NOA01_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HCI0631600.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            31184..31996
                     /locus_tag="NOA01_000371"
     CDS             31184..31996
                     /locus_tag="NOA01_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HCI0631601.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            31986..32471
                     /locus_tag="NOA01_000372"
     CDS             31986..32471
                     /locus_tag="NOA01_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCI0631602.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            32494..33039
                     /locus_tag="NOA01_000373"
     CDS             32494..33039
                     /locus_tag="NOA01_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HCI0631603.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(33112..34755)
                     /locus_tag="NOA01_000374"
     CDS             complement(33112..34755)
                     /locus_tag="NOA01_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCI0631604.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(34884..37112)
                     /locus_tag="NOA01_000375"
     CDS             complement(34884..37112)
                     /locus_tag="NOA01_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCI0631605.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(37145..37624)
                     /locus_tag="NOA01_000376"
     CDS             complement(37145..37624)
                     /locus_tag="NOA01_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631606.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT        11026 a         7171 c         6889 g        12742 t
ORIGIN      
        1 ttgccgttgc ggaaggattt tttaattaac cacagataaa cattgataac gattcgctta
       61 ttgtaatcaa cagattaaat ttcatatgtt catatgtacg gatacatttt cggaccgatt
      121 aattttaacc attctgttta tttgcttacc tattagatat caataaaaaa ctgcttaaca
      181 gctatttcag ctgttaagca gttttaggat ttttagttca tattcattgt ttcttataat
      241 acagtgttca atacttctct taaagcactt gttgatttgc tgaacattgc tttttcttct
      301 tcgttgattg ataattcaac aacttcacga acaccatttt cgtcaacgat tgatggcaca
      361 cctgtaaaga tatctttttc gtcgtattca ccagttaaat aagcagatac aggcaataca
      421 gcttgttcgt tattcaagat agcttttacg atacgtgcag tactcatacc aataccatag
      481 taagttgctt ttttacgatc aatgatctca taagcagcat tacggacttt atcagcaatc
      541 acatctaatt catcttgggc aatacgatgg tctttttcta caatttcaaa cactggttta
      601 ccaccgacag ttgtatgtga ccatgcagca acttctgaat caccatgttc acctaagata
      661 tagccgtgga cgctgcgtgg gtcaactttt aatttcaatg cgatttcttt acggaaacga
      721 gttgtatcca atgttgttcc agtaccgata acacgtgatg ttggcagacc agattcgttc
      781 caagcgatat atgtcaaaat atctactggg tttgaagcaa caacgataat accgtcgaat
      841 cctgatccca tgatttcttt tacgatttgg cgcatgattg acgcatttgt tttaactaaa
      901 tctaaacgag attgaccagg tttttggttg ataccagctg tgataacaac gatatcagca
      961 tctttacatt cttcatagcc acctgaccat acagccacat tttcatcgcc ccaagccata
     1021 ccatctaaaa ggtccaatgc ttccccttct gctttttctt gattcacatc gatcaatact
     1081 aattcattag agattccttg gttgatcatt gcataagcga tacttgttcc aacaaaacct
     1141 gtaccgacga ttactacttt acgacttgtt tttttcatga ataaacagct ccttttcata
     1201 actatttgtt actttcatga aaagtatagt cttgtttatt cattttatca acatgttaat
     1261 tataatagtt gttattataa tttttgatgg aaacgaattc ctgaatacgc ataacagata
     1321 aatgacaaat gtttacacct atatcccctg ataatacaac caaaagttta gaactgaaaa
     1381 taaatccaaa aaaattcgct gcaacgatat aataagacag caatcgcacc taaaacaatt
     1441 ttccttcata tgctttagtg aaagattttc ttcttaatgt ttatttctcc attcattggg
     1501 agtacatgtt gaatacttct tgaataattt gaataaagaa gtcgtttgtt tatatccgat
     1561 acgattagcg atttcttcca acgaaaaact cgtttcttga atcagttcca acgcaaagtc
     1621 gaattttttc tttagaacca gttcttgaaa ggaagcatca aaatacattt tcaatttatt
     1681 actaagatag accggattgt agccaaaatg agctgctgtt tcttttaacg tacaagaagt
     1741 aaaatgctta tcgatataat ctaatactct aagcatctca ttttctgcgc atccatctgc
     1801 tgatgaaaag ctttgttgtt ctaataaatg agtcaattcg atcagtaata gtgatgtata
     1861 gagatttaac gaacggatct tatgactgta tgtatcaaaa taaatcaaca gcatattttc
     1921 aatcaaatgc gtcaaacgac ttgtttttcc gccgcgaaaa tgaatgaatt gtgtatgttc
     1981 ccctttttct tttgatgcat ttatcaaaaa agaagaaagt agattatttg tgcttgccaa
     2041 atgattcaat atctctgtag ataaggtttc ttcttttaat aagatgttga ttaaaatatc
     2101 ctcttgccct gtgtattcga ttctttgagt agcttcttta tcaatgatca ccacgtcact
     2161 ttcttccatc tctatccttt ctccattaat aatttgtaaa catgtaccgc tatacatata
     2221 attgatttca acaaaatcat gtgtatgctc gggaacttcc gcatatcgac ggtgtttact
     2281 gatatgaata tttccctgtt gaaatagatc ccctcggtgg atttttggcg gtgtctgaag
     2341 atggtaagct aagtttaaat cattgatatt cattccttgt tcacgctgca gagtttcgat
     2401 tgattcttcg ttacgcaaaa aaaatgtcta attcagctag ttccaattga ctccctcctt
     2461 tttgtaagcg gtttctttcg cttattagtt cacgctgtac tgctattata tgcaaagcta
     2521 tttttggttt cataaagact ttctatggtt gggacatgaa cttcctgtga ttccgctatt
     2581 gagagaataa ccacttcata ctatattttt gataagcgct tacaaaaatg aaaggaacat
     2641 gcttatgaat aaaaagatga tgaaattttc tctgctgtca gtctctctta ttttgacttc
     2701 tgctggtgca gtcacgtctg caattcccag tatgtcagct acttatgcca cgcgttcttt
     2761 atctgctgtt gaattattaa cgactgctcc ctcttttatg gtcactattt ttgttttgat
     2821 tagtagtttt attgctagga aaatcggaca aaaacaaact gtgatcatcg gattagtgat
     2881 tgcatcgatt tatggtatcc tccccctctt ctcacaaagt tacagtattg tcttagcttc
     2941 aagagctgga ctaggtattg gctttggatt gatcaattct ttggcagtca gcatgattgc
     3001 tgccttttat cacggagatg aacgagcctc tatgattggt tttcaaagtg catttcaagg
     3061 atttggtgca gctttaatga catttgttgc aggacagcta gtacagttcg gttggcaata
     3121 taccttttta gtctacgcca tcagcttacc tattcttgtc ttgtttgttt tgtttgttcc
     3181 tacacctgaa acaactgaac ttcaatcaca tggcacagcc ccaacaagac aagtaatcaa
     3241 aacgaatcct aaaatgatta gttatggtat ttttctcttg ctggtcatcg tggtatataa
     3301 tgctgtatcg attaaattag ccacagtcct gacgacttat tctctaggaa acgaaactaa
     3361 tgcagcgaat atcttaacaa tcatgcaatt ggcaagtatg gctgccggag tttgttttgg
     3421 ctggcttcag acaaaagcac atcgctatat gcttacactt tctttactac tgatgggaat
     3481 cggctttgta ttgattgcca ctgtgataaa cctgtatgtg attgggtttg gtgcgatcct
     3541 aacaggaatt tctttttcgt tattcattcc ttacttattc aaccaagtaa gtatccaagc
     3601 gcccaagtac gcggagagtt tcaatacttc aatcttattg gttggcgcta atttaggtac
     3661 tttcattgca ccatatggtt tgctctttct ctcttttgct tctcttggga gtaaaacact
     3721 tgctgttttc gtcaatggca gtatcttgct gttgattagc gcgcttatta gccttctagt
     3781 agtacacagg acgaatagaa agacagtgat aagagtagat gaagaaataa aagaacaatt
     3841 gacaacaaaa taaatttgat ggaggtcgtg taaaatgaca aatgaaaaac ataaagatag
     3901 atggctttgg tatcctggag actttgagat ccgccatgga ctgctacaaa atttccaacg
     3961 ggaagaacga ggttttgact ggccagcgta ctggtacatg gatgattgcc atcggaacgt
     4021 caagtttaag agatattact ttcttgatca accttccatg tttaaagtga cgatccaagg
     4081 agttgggtat gtagaaatta atggacaaaa acatccatgt ggaaaatggc tgacttgtcc
     4141 agctggtaaa gcaaaaattc gtatctttgt aggacataca tcagggctac cagcgatgtt
     4201 tatcgaggga gacgaagtaa aatcagatat tggctggaca gcaagtaatt ttattgaaga
     4261 atatccagct ggctggagtc cgctttatgt cgatatcgca aaagatccaa atcaaatcta
     4321 ttatcaaaaa gaatgcgtcc atccagtcac tgaaacggag attggcggag gaatcttatt
     4381 cgattttggg cgagcagtga atggctgtgt agaactaata tctctggatg accatatcga
     4441 atctatcact ctatgttatg gagagtccga tgtagaagct atggatatcg attattgcta
     4501 ctataagcaa ataggaatga caagaaaaat gaaagctcgt aaacgcgctt ttcgctattt
     4561 gtttatacca gaggtttcta ctggaatggt tcaacttcaa gtttttcatg aatttttatc
     4621 tctttcgaaa caagctgctt tttcaagcga ttcttcttta ctgaatcaga tctggtcagt
     4681 atcagaagaa accttcaagt tatgtagtga tttgtttttt atcgacggta tcaaacgaga
     4741 tcgctggatt tggtctggag atgcgtatca atcattcatg atcaatcaat atcttttttt
     4801 tgacgaggaa atcaataaac gaacgatatt agcattaaga ggacaaaatg aaatcaagca
     4861 gcatatgaat actatcgttg actattcgat cttgtggttg attggtatcg aaaaccacta
     4921 tatgatgacg aaagactatg aatttttacg catcgtctac cctaagatgc aatcattaat
     4981 gagatacttg atggagcaaa cgaatgaatt agggtttatt tatgggagag caaaagattg
     5041 gatatttatt gattggtctg aaatggataa agaaggaacg cttgctgcag aacaagtctt
     5101 attactgaaa gcctatcatt ccatgatcac ttgcggagat atcttagctg aaacatatgg
     5161 agagaacgaa aattatcata ctcatctcta tcaagaaaaa tatcaactat tgaaacaaaa
     5221 cctatttgac tacttttggg atgaagaact tggcgctttt atcgattgct atgaatctgg
     5281 gagaagaaat gtgacgcgtc atgcgaatat tttcgctgtt ttattcgatt ttgctaacaa
     5341 agaccaacag caatcgatct taaagaatgt cttattgaat gacactatca cacagattac
     5401 tactccatat ttcaaattct atgaacaaga tgctttatgc aaactcggac agacgaatat
     5461 cgtctaccaa acgattcttg attattgggg agggatgtta tctcatgatg cggtaacttt
     5521 ctgggaagaa tacgacccta ctcaaacagg aaatgaaaag tacagtatgt atggagatcc
     5581 ttatggaaaa agcttatgcc atgcatgggg agcaagccca atctatcttt taggacgcta
     5641 ttttatcggt ttgtatccaa caaagccagg ttatgaagaa tttatgatac aacctaatct
     5701 agattcattt gaaacactga attgtacgct acctatcaaa gatggaaaga tttcgcttga
     5761 aaaaacaaaa gataagatcg tgattacttc tgatagaaca ggcggaacag tgaaaatgaa
     5821 tggtaaaagt tatcctcttc tagcaaaaca gccacttgtc ttaacaagct attcaaaaat
     5881 aaatgcataa aacaaaaaac tgcttgcgac atatcagaag actttaaatc ttcttctgat
     5941 attgtccaag caatttttat tttgaagttt gcttaattct gttctttaga aacaatgttt
     6001 gttctttttc cggcaaaaag aaagctagat aatacgacaa gtaaaaagaa agcgatgatt
     6061 ccaactgtca aggctatttg accgtattct cctagtttcc caagtacaag tcctggtact
     6121 agataatcag tatcggcaaa tgtagaagaa gcgattttaa gatctcctaa tactggaagg
     6181 ataaataatg gcagccaact aatcaataag ctattgaaag cagaacctaa taccgcccct
     6241 cttctaccgc cagtcgagtt gccgaagaca ccagaagcag ctccgcagaa gaaatgtcct
     6301 actaccccag gaatgataac tgtagtttgc aagcctatca tagcaaacat ggatagtgtt
     6361 cctacaaaga agctaacaaa aaagccaatt aatacagcat taggtgcata tggaaaaacg
     6421 ataggaacat ctaatgctgg tttagaatcg ggtacgagtt ttttagcaat cccttggaat
     6481 gctggaacga tttctgccaa caccattcga accccttgaa ggacgataac aaacccagct
     6541 gtgaatgttc cagcttggat cagtgcaaag attacagcgt tgtctccatt gcttagttct
     6601 ctttcaacaa atccaggacc agctaacaaa gcaagtgcga catatacgat cgacatcaag
     6661 agcatgatcg atacagtaga atctcgcaaa aagctgaaac ttttaggaat ctttatgtcc
     6721 tctgtagaac ggtctttgtt tccaaaaaac tttccgatcc atccactaga agcataaccg
     6781 atatttccgg tatgccctaa cgcaacttga ttgtttccag tgatgttctt cataaacggt
     6841 tgacaaagag caggcgtaat agttaatagt gttccttcga agaccccgcc taataagatc
     6901 atccacaaat ttgcagtaaa ccccgcgctt actaaaatga ctgctgttag acaagaaaca
     6961 taaagcattg cttgacctgt aagaaaaatg aatttatatc ttgtgaatct agcaagtaaa
     7021 atattcacga tcatccctac tagcatgatc aaagcagtag gtgtaccgta ttgttgcaaa
     7081 gcaatcgcca ctactgcttc attgctagga actactcctt gagtatgcag agctgtttgg
     7141 aacatcgaag caaaaggggt tagtgaatta ctgattaaac cagcaccgcc gatcagtact
     7201 aggaatccga caaatgtttt gatagttcct tggatcactt ctgaaaattt cttcttctga
     7261 agaacgagac caagcagtgc aatcaaagaa acgaggatgg agggctgact gacgattgac
     7321 acaaacaaat tcaacaattt atccattgat agttcctccc ttgaatcatt tttattcttt
     7381 tgacaagatc ttcgttactt tttcttttac ttcttcgctg tcaatgatcg attgcaaagg
     7441 tacgagcttt tcatcaggta tagaagttag cgtatctttc aaagaatgct caatgaagaa
     7501 ataatcagcc atatcgctag atacacttcc aacatccgta tgacttactt caaactcgtc
     7561 cacatctgct ccaagatctg caagtgcttg tttgatgttc atctctacca taaaacttgt
     7621 tcctaatcca ctttgacata ctgcaaaaat tttcatatca ttcgtctcct tttcttatga
     7681 tcgctaatat ttcttctttt gttgatgcgt gtaataaatc ttgaagattt tgtttattag
     7741 aaagtagctt tgttaaattt gccaaagccc gaagatgtgc ctcattatcg atagcagcta
     7801 aacagatgaa caaactaatg ggatgggctg gattatcaag cagatccaca ggttgttgaa
     7861 gtttcagcaa agacatccct acttcgttta cgccatcttc tggacgagca tgaggtaatg
     7921 cgatgtgatc gccaatatgg ataaacgcac catactgctc cacgcgctcg atcatagctt
     7981 tgatataact ttcttctatt ttattttctg ataatagcgg ctgtgcggca aatgtgattg
     8041 cttcttgcca gttttttggt tgatccgata attggatcat ttcttctgtt aataattcag
     8101 atagcatagg acgttgatcc tccttctttt ctaaaagttt gttcatttta cgatttaata
     8161 cttgatagag cttttcttcg tctacgcctt cttttaattc gatataaggc agtagcgccg
     8221 acaacaattc ttgggcagaa ggcattgaaa atcctggtat cagccaatct ctttgtactt
     8281 gattgatcag ctgatttttt tccaagtcag tcatgattgg atgcagaaca tagagagact
     8341 ttttcttatc agtaagtgtg agaggtacag tagaaaaaat aatatcgtaa gaagtttcgt
     8401 caacctcgtg taagcggtca gctgaattag tctctgtaaa aaagatcatc gggaataatt
     8461 gctgaagttc actttttaag atcaatgaag agctaatgcc acttggacag acgatcacgg
     8521 ctcttatttt tctatttctt tcttctgcgt ccgcttttct gatctctccg ccaaatagta
     8581 tagtgaaaaa accaatctct tcttcaggga tgctcttccc tattcgtctt tctaacggaa
     8641 gtaatgcttt tcttgtcatt tcgtagatgg aagcatattg atgcttaatt tgatcgatca
     8701 tcacattagg taaataaaaa gaataggaaa tacgaaaata ggcagggacc aagtgataaa
     8761 agacattcat caataattca cgaaacgaag caaattctac ggccatcagg aattccatgc
     8821 gatggatgac ttctgaagaa cattccagca aaaaatcaag tgcaggctcc tggacatctc
     8881 cttcaacgat cgatgctaag caaagagaga aaaatataat ctccgaattt tgatgttcgg
     8941 aatagccaag gccagtcaat acttgtcgac taatgtcagt gactgcaaga gaagccaaca
     9001 gttcagcatc ttttaccagt tccatggatg tttttccaat tcgaatggtt gtcaaactca
     9061 aataataagc taattcatcc attcgatttg gtgcgaatcg aagatctgat agtgaaatcg
     9121 ttttatctag caaatctcta actttggcgt aataatcaat ggaaagggtt gcggttaaat
     9181 acgtgagccc aaatcttcca gcaagtgact gcatcaaacg agcagtgcga ttttttgctt
     9241 ctcttcttaa tattgcttct gaccctagca gcctaaaacc agttttccta gtatacgaca
     9301 gctgacaatc ttccacttct aattcatttc ttatcttttt gatatctgac aggactgtcc
     9361 ctttagaaac ctgaagaaaa tcttgaaaat gaaaaacaga aacagacgtt gcagtcgaat
     9421 aaaccatcaa ataaagaaga tctcttcgct cttgttcggt atacacagct tcaaatcgag
     9481 taatatccaa aaatttttct gcccagtcga ttgctcggaa tttctttata cggatacggg
     9541 tatcatccac atattcggca aaaggagtta attgtttgat cgcctcgctt gtatttactt
     9601 cgatgatctc tgctgctttt tctagactga tttctcttgg tgttttcagg agataactaa
     9661 ttttgtagtt cacagcgctg ttcacctcct acttatattc tagtatggta gcgcttttaa
     9721 atcgaggggt gtttggtaca acttacagtt ggattttttt tacgactgta ccgaacgatt
     9781 tctatcgaat ggatacagaa aaaaagcagg tgaccaaatg tctcctgctt ttatgaattg
     9841 atttcgtccg atcaatgatg atcatgttta ttttttgagg tcagttcttc aaatttttct
     9901 ttgacatctt caaccgcttt ttcgatatct ttttcaaagt gattgggctc attaggcttt
     9961 gtttcctctt ctacagaatt tgaaccagat aaagctgttt ctttattttc tgtttcacca
    10021 gagtttgtgc cagctaatga ttcttctttg tcttctgttt caccagaatt agttcctgct
    10081 aaagccactt cttcttctgt tttaggggcc attgaacctc ttaatcgttt gtgtttttca
    10141 ccgtcataat ttgtcatgtt ggttcctcct agtagttcta tactttttca tcgtacaata
    10201 agacagttta tgcgtcaaaa aacaaacccg attcttacaa atatgtcata tttttccttc
    10261 tgattcttga atgcttttga ttccttcttc caaaattttc atttgtaaga ttgtttcttc
    10321 atctttgatg actttttctg cgccattaac taatgtctcg taaatccctt cgtagacacg
    10381 gctgtaatca ccaacctcgg aaattacttt ttcttcatga taactccctt gttcatctat
    10441 ataggtcaat acaccataat gttcgggcaa atcgatacca aagtcttcat gatcaggcat
    10501 gtaaaacatt ttcagatgtt cttcttgtcg atcttttgtt tgtttcacga acatcccttt
    10561 ttttccgtaa acgacgaaac ttggacgttc tttgatgcgg aaataactgg attttatcga
    10621 aattttcata tttccataga aaaaatccag atcgaagtaa tcattcattc gtcctttccc
    10681 taaaagttga cgtacatcat agctgatatc atctggttcg ccgaaataag aaagaacttg
    10741 atctactgta tgacaagcat gtccatataa atagctgttc gcagtagaaa attcatgcgt
    10801 attttctggt acttccggtc tgaaataatc ataatgcatt tctacttcta aaatatcccc
    10861 taattttccg ctttcgatta ctttttggac tgtcaagaag tcagaatcga accgtctgtt
    10921 ttggtaacat tggataaata gacctttttc ttttgctaat gcaaataact cttctgcttc
    10981 ctttgacgtt tctgcaaaag gtttctctac tagtacattc ttaccatgtt cgagtgccat
    11041 ttttccatat tcataatgaa attgactagg tgtagtgatg acaactaatt ggatctcagg
    11101 atcatcccaa agatcagaca gctgatcagt atattcgatg tcttcgaatg ttttccacac
    11161 tggttcagat ggggaaaaaa tttttttgat attgattttg ttcgtaagtt ttcttgaaaa
    11221 aggcaaatga tagcgatttg tactctttcc attgccaaga taagcgattg taagcatgag
    11281 tggtgcctcc ttttattgat gttttcagta tatcctagaa aagcgcagaa agtatttata
    11341 atagctattc tggtaaaaaa gttcagaaaa acacttgtta tcattcggtt tggtgaaatt
    11401 aatgttcaaa gtaaggataa aaatcaaaaa gatagtagcg gaaccaagtg tttatcagta
    11461 agatagaatt tggaggtcag atcatgctta ttgaagaaaa actaacaaaa caagagttgt
    11521 ttaccacgac agagaaacgg attgtcgatt acatccgtag gaatatcgaa gctgcagtct
    11581 acatgacgat tgaagaatta gcaaaagcta cgtatacttc ccattcagct atcattcgtt
    11641 tatgcaaaaa aatgggattc agcggattta aagaattccg tttcgagctg gggcgggaag
    11701 ttcatcaatt gattgctcag ttcaatcaaa cgattgatcc taattttcct ttttcaagtg
    11761 aagatagcac tcagcaaatt gcaaagaaaa tggcggattt atcgatccag tcaataaaaa
    11821 aagcacaatt gcagattgaa catgaagatt tagatcaaat cggtaaagta ttgaacaagg
    11881 cacagcgaat ctttcttttt gcaaaaggtg attctcaaat cacggcaagg aaattccaga
    11941 ataaaatggt caagttgaat aagtttttga tcatggctga agaatacagt gattcttctt
    12001 ggaatgcagc caatttgact agcgaggatt gcgccatttt tattagctac agcggtcgca
    12061 tccatcatta tgaacgaata atgacctatt tgaagcatgt agaggcccct accttgctga
    12121 ttaccggaaa tcagcattca gaaatggcca aacaggctga tatgtgtctt gttatatcgc
    12181 aggaggaata tgattttgct aaagtagcga ctttctcctc tcaaattgcc ttcgattacg
    12241 tattaaatac gttattctca gtgatatatt ctcaaaattt tgaagagaat atccgcaact
    12301 tgaaaaacaa acaagaattg atgcaaaaag gccttcttaa agaatatcgg aaaacttaag
    12361 aaaagatcct catttagtaa taagaaagat agtgtttgat aggctctctt tgatatcatc
    12421 ataaaaacag gctgaagttt tctttttcaa acctcagcct gtttgtttac ggaacgattc
    12481 gaatagatat attatagaaa tgaggaatag ctaacttctg tttcttattt tgaacgattg
    12541 acgaaaaaga tatataagaa ggaggaaaat cagtatacat gcagctaaca atccatacag
    12601 gaaaaatcgt ttgttctcgg atactttctg tacttctttt tgaacttctt ttgcatgata
    12661 aggaacccgg tgccctctga caagtaagcg atgactgttg accatataag gtgtacaagt
    12721 taggagtgtc acgaaatcct gccctttttc gattctcaga tcttttgtat cagtcggttc
    12781 taccgtcttg atctgatcga cttgataagc gagtgtccgt ccattgattt cgatatagaa
    12841 agaatcacct aaagtcagtt tcggtaagtc tgtgaacaac tttgcttgag gcagtcctcg
    12901 atggctggaa ataacggcat gtgtattttc tccccccgta ggataagacg ttccttccaa
    12961 taatgaggca cctttttcta gcagcacagt attcgtttca tcaaaaattg gcaaatggac
    13021 attgatctca gggatcgtca aaataccgat cgtatgtttc tcaaaatagg ttctatcctt
    13081 tttcgcaggt tcttttttct tagtaaaggg atctgcacct ggatttccac cttcttttgc
    13141 taattgctgg tttttctttg tcatgttttc ttggatttta gccatgactt cttcattttc
    13201 tttgaccgct tgttgctgat aatgagagat gatctgctga tccagataat tattcagcgc
    13261 gtcgctaaca aatggataag ctagtatccc tattcctgag agaagcaaga aaagcatcag
    13321 cagatctaga agtttccttt tctttggttt catttgtgtt ctccttgtga tgactgtttg
    13381 ataataaaag aaaaaggagc atttgcgtaa tccgctgctc ctttttccaa ttcttgcaaa
    13441 aagactaaat ctgactgtgc ttgcgtctag caaagtacag tccagcaatc agtagaagga
    13501 ctactcctgc accgatatac acatagattc ccttaccgcc tgttgaagga agtgtacctt
    13561 tgtgtttatt tggtattttt tcaggagaaa tcaactgtcc tgctgttaca taagattgtt
    13621 catcgatagt aaatgctaca cggtttgtta atggaacata tttttctggc gctttcgttt
    13681 cttccagata gtacgtgcca tattttagac ctgtcacatc gattaaaccg ttacttgtgg
    13741 ttgtaaaaac cgttgctgat tctttcgccg atacccagct gacggctttt gtggatgggt
    13801 cgatcgataa atatttcgct gtgtcactat cttgatcacg aacgacgaat tctgctccag
    13861 caagtgtttg gtctgatgta acgtcaccat ctactttaac aaatcgtttg cccccagtaa
    13921 cgacatcgac tgacggtggt gtttgatcat ttgtatgccc gttatcgaca tttgcttggt
    13981 tagaaaatcc tttggttgga tctgcttttt cgttcaaatg catatagtaa acgaatttca
    14041 atgtaccgcc aggagttaat gaaggaatat aattcggatc aactgaaacc gtgaatccgt
    14101 ctgtttgctc agtgacagaa taatttactg ggtcgatttc tttatttcca tcatataagg
    14161 cataagcata cgttcctgaa gaatcattat caaatgttaa agcagcgtca tgagtatcga
    14221 taagtttgaa tgttgtgtac ttgttttgcg ttccttcttt atcagcgata cctaatggga
    14281 tattgacaga aatctcatat tgaatttttt caccgattgc gacatctttt ccattcaatt
    14341 gaggtgttgt tttatcaact ttagacgtat catttttgat ggtcttttct actggtgttt
    14401 ggctatttgc caggatctca aaaggcgtga ttgtttgctt ttcaatcatt tccgcattat
    14461 caggagcttt tacttcttct aaattatatt ttccaacttc taaatgatta acgatcaact
    14521 ggcctttttc aatagatgcc tcggcaaagt cattgttgcc gatgtcataa gaatgaccag
    14581 taatgaaatg tttggctttg gtttgatcag ttgtccaagt gtacaatcca tctgtgacac
    14641 tttggatgta ttttttgacg cttggtgttc cttcttcttt agaaataata aattctgctc
    14701 catttagtgc ttcgttttcg gcagtaccga tttttgtaac tttcaacgtt ccatcattac
    14761 agactgtatt tttagggtag agatggatag tatctagttc ttctgtcccg tatttataag
    14821 agccatctgc ttgtttgatc atctcatata cagggaaagc taaaaccatg tttgcggcag
    14881 ctgacactcc gtcttttggt tcttctttga tcgtgtagac tgcatctttc ccattttgtt
    14941 ttttaggtaa agataaagtg acatttccat cagcatctgt cgttcctgaa gcaactggtg
    15001 taccaggagt caaagactgg actgcttgtt ttgctgcatc cacggacgct cctttatctc
    15061 gttgcgcata aaattcttga gtgacgttat aaactgaaaa tgaaatatcg gctaatcctt
    15121 ggtattgatc gaattcgctc atttctttcc cgctgttttg gattaaagga tcgggtaaat
    15181 cagtcatttt ctttttatga aggatgactt gagctgctgt ctcctctgca aacacttttt
    15241 ttgcgccgaa gctgtttgtg agtaatggta aaataaggaa aaggactcct aacataacgt
    15301 ttattttttt atgatttttc attttttcac tcctgttctt tttttgataa acaaccagat
    15361 tgcaaagctt gcgaatacca taccaacaac gcctaaaagt gtatttttcg tttcgtttgt
    15421 ttttggaaga aatggacggc tattttcttt tttattgact actttcaata tactgtccgc
    15481 ctctttatcc tcagaaagaa aagttgaaat ggtaaacgga atttctgttt cacttaaaat
    15541 ataaccagaa ggtgcttcga tctcttttaa tcgataactt cctgttttca acccggaaat
    15601 tgaaaaagcg ccatttttat cggaaatcag gtgaagcgca tctgattcgt tctttgtcca
    15661 cttgtatccg ttggctgttt gctggaggta atttcctgct ttgtttttaa taaggaatac
    15721 tgcgccagct aatcgtttat ctggtttatt tgtctcgatt ttgacaaatt gcttactccc
    15781 tgtatataca gagactttct ttgtactgac accaaaacca aaatccagtg ttccgttgtt
    15841 gttgatttcc ttgttcgcct gtgctgtact gcttagctgc atctgataag aaatggtcag
    15901 tttttttcca gcatattttt ccaacgaagg gatcgaaaat aaaacatcaa atccgtgagg
    15961 tttcttgtgt atgacaaagc cttctgtcag cttttctcca ttcgatgaaa tcgttaaact
    16021 attaggtaaa aacgttaatg cttcatccgc actatctgac aattcgaact tttgatagtc
    16081 aaggatattt acaggaattg tcgtatgcaa ggaataagtg attttttcac caatcgtaaa
    16141 atcatttcta ggctcttcta ataccttctc aaaaggtgga tcagggtagt cgttttcttc
    16201 atttttagga tacaaatgga tagttgaaag tttttgattg ttttgtccat gtacaggaag
    16261 aacaactacc atattctctg ccttttcttt gacgacttct ggtactttgg attcaatgaa
    16321 aagatacgct ttatctcttt tttcttgatc tttagcggcc agagaaaaag aagcgatacc
    16381 atcttcgttg ttaagagttg tagttgttgc ttctgcggta aacataccgg aagacgcacc
    16441 gttttttgcg atctctgctt gtgcttcttc taccgttttg cccttttccc gtagatggta
    16501 aaaagaatct gtgacatcat aaacttggaa tgtgacacca tttaatcctc gatacgtttg
    16561 taataaagct ttttcttgtc cgtcatttgg atgatttttc ggcagttgcc cgtttgggaa
    16621 aagcaatttg tgtaaagtaa tctgaaccat ttctgtttct gtttctgttg ctacctgagt
    16681 aaaagctggt ttaaatagta acaagaagag acacatacta agccaaccaa gttttttcat
    16741 atttagctgc cccctctttc ttttttcaaa aaaagatacc aaatagaaaa cgcacaacca
    16801 atcatcccta ctagatagat tcctaaacgg ccaattcctc ccgtttcagg taatggtact
    16861 tttgcctgat tcgtgatgtc taaagaaatc tggttgtttt tggctcctgg tgacagaaca
    16921 gattcatgat cttgtccatc cacttgaatt gacccatctt cgtgtataac tatagtaact
    16981 ggctctttta gaccttggta tccttctggt gtaaaagttt cggtcagcgt ataagttcca
    17041 ggttttaaat tttcgaatag aaaggtatct gtttcttgtc catcttttgg cgattctaag
    17101 tccatttctg gtccctgcag tctgaatttt gctcctttta atgtctgtcc gttgtcttct
    17161 tttttgtgta ccgttagatc aaacgctttt agtcgatttt gatgagtgag cgtccataca
    17221 ttatttgtca tctcggtatt ttttcccgtg taaatgattt ttccatattt atcgatttgg
    17281 aattcataat ttcccggcga agtatcatat ccggcaggac cagctgtttc aacgactcga
    17341 tatttaccag gtgtgagtga atcaaaactg gcaagacctt tttcattcgt tgtttggctg
    17401 tcaattggct gataagtacc atttgcttct tttttctgta gggaaaatgc tgctccttta
    17461 agatttatct ctttgtccgt cccttgcatc gcatacttac gtactgctac aggaacttca
    17521 acaaaaggat tttcaatagt caactggatc gtatcatcgg aagtagtgac tgtttttcca
    17581 tcaatggtta ccgtaccatc agaagcgatc ttgatttccc aagtagtctt tttgcttaat
    17641 ccatgccctt ctggagcttt tgtttctgtc agcgtatagg tcatttcttt ttgcaatttg
    17701 acattttctg gaagagaata ggttccgtcg ccatgatctg ttagtaaagt atcaatagaa
    17761 tctcctgtta atttgaaaac agcgccaata agatcctttt cacctgtaga ggaatttttc
    17821 gttattttca agtttaacgg tgtgaattgt ttagtatttt tccatgtcat tgcatctatt
    17881 tgttgagaat cgtatcctgg tacaggtaat tctttgattg tttggtaact gaatgcttga
    17941 ccttgattat tgtattgagg tagtgataat atctcttgat aactttctcc gctatttgca
    18001 gataatttgt caatgtctgc acgttcccac gtatttgttg tatctttagc tggtttaatg
    18061 attcgaatat atccgttttt ccaagctgca gcatttgtcg tatgttcccg ttgaatctca
    18121 aaagtaactt gatctggacg atcagctaga ttgttgtcaa attcttccca taactttttg
    18181 atgtgaagac tgactccggg agctttagca gatggtatac cgaattcagc taattcattg
    18241 gtatcaatgt ttggctggaa agttgtcctg ccgttcattt gataccagaa atttggatgg
    18301 aagtcctcat tttctgtttg gattctcact tggtaatgga tttggatttc ttggtctttt
    18361 cctaaataga tctgattgct cttgatggta tttccttcta tggaaataga tggagtgacc
    18421 gttgtaggac ttgtccccac actcttgact gaaagtgtac caggctgata aatgaatggt
    18481 tctgcgattg gatcatttat ttgtccattg cttacagttg ctgagatctg tacagctttt
    18541 ttggctaggt attcagagat atctgttgca tgatcagctg attcatagta aagatcgcct
    18601 ttttcatctg atgaaaccat ttgtcgcata cgagactcta cttctgcttt tgagagacca
    18661 gctgccggat cgctttgaag ttggatgcca agaccatgta tttcgattcc tcgttctttg
    18721 agtgccatcg cttctccgat ggttgcgata aacgtactgt cgattcttct ggatagattg
    18781 ttttggtcag gtgcatcata gattcttgag attagagaag tatttcccgg ccgatcttgc
    18841 gtattagaaa actgagttcc gtaataattg ctgcttgatt gtgcgtgtac ccgctgtact
    18901 ttataggaaa atgttggtac accatccgtc agcaaaacga tcactttttt atgtccattt
    18961 ggaacggata gcatgcttcc tgcatctctt agtgcttttt gtgtaaaagt accaccattt
    19021 gtccgtgaag gtgtaatgga ttttacttga tttttcactg aatcaaatga acccatctgt
    19081 actgcaccgt tactgtagct atatccttcg cttgagtagc cgacatatcc catattgatt
    19141 ttgtctgtga taccgctatc tgctaaagta tcgacaaaac gatcgacacc aatctttact
    19201 tcaccgatcc gattattgtc gttcatactt cctgaccagt ctacgaccaa tacgagatca
    19261 agaggcgtga tatctttttg tacatttcca cgagcattca aataaacatc aaacaatccg
    19321 ggtgtacttg tttcctttgc atactttcgt atcgcatagt ctgcttgatt accggttcct
    19381 ccatattcga tatacgaatg ggtccgatca ttaggatctc cattccaaga atttatacca
    19441 tcccattgat ttgttccgtt tttattgcct tgatgatcaa gaacatttgt atttcctgta
    19501 ggttgccaac tggctttagg ataagtgccc tgatcatctg ttgtatattc tgtaggaatc
    19561 gtttgattag atggcacact cacagctggc aaggataatg taggcaatgt aaatgggctg
    19621 acagaatttt cgttttcttt tgatgattct tcttctattt cgtctggctt tggtactgac
    19681 acatctgtat ctttcttatt ttctgtctca tcaataatgt catattcttt attatcgacg
    19741 atcagcttgt ttgcctggct gtggtaaagt gctggatcga ttggaaaact cagatcgata
    19801 gtttgtttag tgtttgatga gatttccaac gtaattttcc cgctatttac ctgataagca
    19861 tagccatctc cttcttttct ttccacatca cttaagtaat tttcgactag accaatttct
    19921 ttttctatct tttcagcact ggtgttattc gcatttagct tgatgttcag tgcttttttt
    19981 ccagactgat ccgaaatgac ttcagctgaa attgtttcta aaactgttgg atcatctgta
    20041 gtgtctgcta ttgcagataa tggcacaaag tttgataaac tcaatattat cagcatgaaa
    20101 ataactttca gtttcttccc tgtggtcgtc atttttcccc tcctttttct ttttttctgt
    20161 tttgacgtga atagtgaaaa ttattaaaat aaacaggcgt tacataattt tatttatttt
    20221 aatatgataa ttattatacg aagggcgcta aagggaatca caccaatttt cataccaatt
    20281 ggtttcattt atgaagttga ttccttcgat taatgagcgt ttcattgaaa gaaaagggtt
    20341 gagggagaaa aacatatgat caggttgttg gaatgaaact atagaaagat ggaaaaaatc
    20401 attcgaattc tgtccatttc tgacaagtgg atgactaaaa aagaacttgc caaacgaatt
    20461 ggtagttcag aatctacatt tgtaaggtat atcgaagaga tcaaaatccg ctggggaacg
    20521 gatgtaacga ttcaatcttc acaaaaattg gggtatcgtc ttgaacattt gaatgtacca
    20581 gcttatttaa atgttctgat ggaaatgtcg caaagttcta caaacagtcg tttattaaat
    20641 gagattattg tcaatccggg taaaacgatt gagtattatt gtgaaaaaat ttccatcagc
    20701 cggtcttctt ttacacgtaa gttgaaacaa tgcaatcaaa ttttagaaag ctatttatta
    20761 aaagtgatcg ttgaccaagg ttttcagtta actagcctta aaagtgagct ccagttgcgt
    20821 atctttgtta cctttttctt tttaagttac tacggaagac gcacgttgcc atttccaatc
    20881 gaaaaaaaga agatcaaaga aattatggtc aagaataatt gtcagatgaa tttgtttcag
    20941 gaagaaagcc catatgagca atctttcttt attatgtatt ttgttgtgca tctcatgaga
    21001 gaatcccaag gaactccatt tgaatggaaa gctggagact cttatgtaca gttgataaat
    21061 atcgataaaa aagacttctt aatcttgaaa aatctcttag gaaaattgaa gtacagcagc
    21121 tatataaaag tagtgaacta ttttgtcaat acgatttttt cagaattttc ggagaaagaa
    21181 aaaaatgaaa tcagtgagca gatacgaaaa aatatgtcct actgttcgtt ttcatccgct
    21241 gattgcaaag aaaaaaacga agaatatgtt gttcaattat taacgcaaat ttattttttc
    21301 tcccgtattt tcccatacga tacgactcat ttgacgatcc gtgcaactta ctttgcacag
    21361 cagatgcaac accgccagat acgtctatat gctggatttc gtgcagaact tcaattttat
    21421 tccactctac tgaatcagaa tctgatttat tatttatcta gctatatctt ttggatgctt
    21481 atcggtaatc ctgaaattca ccattatact tctgataaaa aaatactgat tatcagcgac
    21541 ttaagtcttc gacatagtca atatattgaa gaatatatca gtgatattct tgctgtgcat
    21601 aaaattcact ctgagacaac tgctattact gaagatcagc tgtcgaaata caatcttctc
    21661 gagtatgatc tgatcgttac gaatcaaccc attttgaatg cccatgttcc tcatatattg
    21721 attgatgata gtgtgtcatt cgcaaatgaa gaagaactca tccgcttatt cgagttatga
    21781 ataatcaaaa aacagctatg tataaaccac cctgataaaa atttcaggac aaatacacat
    21841 agctgtttct ttttattgta ttccttcagc aatccaacga gatttatagc gaaagtcttt
    21901 taacaactct tcttttgtcc aattatttgc aatgagatcc gtcaaagtgt tcattacgag
    21961 ggtcatggaa aagtatagga aactaactgc ttcttccgac gattgaatgg agaaatgatt
    22021 gattcttaat aattccagcc atttttcgtt cattggagaa tcagccgata gttctctacg
    22081 tttggttgtc tgctgatcat ttcgctgggt taacaagtat aacctttgcc aatagatact
    22141 ttcgtgatct aagtcgcagc accactctaa atatttttcg atccccaaaa agaaatgttg
    22201 tttgttttcg ctaatgaatt ttaagatatc ttgatggact atgtgagaac tgcttttgat
    22261 cgtatacaaa taagcatctt ccaaatcttg aaaatattta taaaaagcac cgcgcgacat
    22321 ccccatcttg tcaacgattt ctgagacttt gacttggctg atgtgctgtg agcaaaatat
    22381 ctccagtaat aggtcatcga ttctctgttt ctttgcgttc gataaattga aaaaagtttc
    22441 ttttggcatc cacatctctc ctttatctct gattatacgt ggatagccct tcattgtcat
    22501 gaaggattca ttccttcttt ttctcttctt ctgtcaagta cccatcttcc attcgataaa
    22561 cgcgatcact ccattgaatc atacggacat catgagtaac cataatagtc gctttttgtt
    22621 tctcatgagc ttctttaact agtaatttga cgacttcata tgcatgatct gtatccaaac
    22681 tagctgttgg ttcgtctgct aagatcaagc ttggctcatt atataaagcg cgagcaattg
    22741 ccactctctg tctttctcca ccagaaaggt ctttaggata actattttta agtgactgga
    22801 tatctaaaga ttgcagcaag gaatcgattt ttgttgaatc ttcttttttt gaagtaactt
    22861 ggtcaactag ttcaaattgt tcctttaccg ttaaaaaagg aatcaaatta gagctttgga
    22921 gaataaatcc tatttcttta aagcgtaaat ctgctcgttt tttttctgga agctcagtta
    22981 gtgagtgacc gttgatcact atttctccgg aagtcggtgt ttgcaatcct cctgaaatgg
    23041 taaggaaagt acttttacct gaaccagaag gaccaatgat gctgacgaat tctccttcct
    23101 ttacataaaa atcaattgct ttcaaagctg agacctttgt atgaccagaa ccaaatgttt
    23161 tggtaacttt tttcatcgtt aatatttttt tcatttttat taacctccta ttgctgtaat
    23221 tgggtctact ttggttatcg tacgtatcga aaacaatccg cctagcattg cgacaatgat
    23281 caaaatccca ctatatagca accattgtga ccaaaaaaca gcaaaaggca tcgcgcttgg
    23341 taaaatcaga ctagtcaaat aagcaaacaa aagagcaagc agaacgccta ccaccccaac
    23401 aataaaggac tgtgctacta aagatttagc gatgaagaaa tttttgatcc cttgtgcttt
    23461 cattactcca aaaatagcag tcttctgaag tgtcatcacg tacatgaaaa taccgaccac
    23521 ggcagcaacc actaagaaca aaaagtatat catcgcattt aaggtcatat tttgggcaga
    23581 atagccagga atactttcaa tgaatgtccc aatagttaat aactgtagac ccttcgatgc
    23641 tttttctttc gaaattttag ctgaatcctt tattacgatc ccattgatcg gctgttcttc
    23701 ttcagaagca aacggctgtt gtccatactt caaagcagtc cacgtatcta gggacgcata
    23761 gatgacggga gaaactgtgt aataagtttc aggaaagata ccgacaatcg tcaaaggatc
    23821 gtcgtaactg ccaattttga tcgtatctcc aattttatag cctgaatcag ccagattttg
    23881 agagatgatg atttcattct ttgttttaaa ttcatgccct ttcgtgagtt ttggtaataa
    23941 aaatgcttct ttcgttgtgc cgaaaactgt aacattttct ttgttttttc ctttgattgc
    24001 tccgctgtac aatccgattg gtgctttctc tcctccttca atgtatttca gatctcctct
    24061 tgttaattgt gaagctgcaa atacttgatt tgcatcctcc gacaaaacaa tctcttgtgc
    24121 gtcccaatca tcgattgctt ttttaaactc ttctgaaagt ccattcgcta gtcctgacaa
    24181 catgaatacg acatacgcaa tcaagaacat aataccgacg atcaagccat atcgtaattt
    24241 agaatagcga atttctttga tagctagaaa catttgtttt tcctccctat gatagattga
    24301 tagtgacaaa atgtcacttg ttgaatagtg taactctaaa gtgacaattt gtcacttgtg
    24361 ttgttttttt cttatcaata aattgtctaa gacatctttc gataaaacct taacatgagt
    24421 cataaaaaat ttatttaagc cttatttatg ctttccatga taaactaaag tgaccaaatc
    24481 aatgaggtgg gaaaatgatt aaactattag tagtagaaga cgataaagta ttatccaata
    24541 atataaaaga aatactcgct gcaatcggcg agataacaca agtttatgat ggtagtgaag
    24601 gactgttcga gttggaaagt ggagtttatg atcttgcaat cttggatttg atgatgccgg
    24661 aaatgaatgg atatgaagtg ttagcaaaaa tacggaaaga aaaaatacag actccagtat
    24721 tgattttgac tgcaaaagat agtttggaag ataagattga aggatttgaa cgaggtgcag
    24781 atgattatct aacaaaacct ttctatcgag aagaactgat catgcgcgtg aaagcattat
    24841 tgaaacgctc gctaggtctc tttgatgaaa atgttctgaa gtataaggat atcagttgtg
    24901 atttaaaaag caatgaagtc ttgtataaag aacaagtttt gcccatacaa ggaaaagagt
    24961 ttgaattgct cgtttatttt ttacaaaata agggaatcat tttgacaaaa gagcagatat
    25021 ttgaccggat ctggggattt gattctgata cgacgattac agttattgaa gtttatatga
    25081 gcaacttacg aaaacatctg cgaagtactg gattggataa agaaatcaaa accttacgta
    25141 atgtcggtta tatactgcaa agggtgtgat gcaatgaatg gagatattac acgaaagcaa
    25201 cgtagtcgct tttttgcgac aaatgtagca gcatttgctt ttatcttttt tcttttggga
    25261 ttcattactt tacagatatt aaaccaatct gcatacagag aaacagatct gtcattagag
    25321 aaaataaatt ttgattctcg aacagtacaa ttagagatac aaagatatca gcaaggcgat
    25381 ccctttttaa acccgactca aacggaacaa aacagaacaa acatggaaca gccattaatg
    25441 gaaccatcaa atgaccgttt taacactcaa gtgatcttgt ggtctaaaaa tggtgagata
    25501 ttgaataaag cagctattgg tgggcgtttc aaccaaatca aagaattaaa aatggatacg
    25561 aaaaatctga ataccatcca aaatattgaa ttaaatgaag acagtggctc atctgccctc
    25621 tctttccgtt cgattacgaa aaaagcggag acgaatcaag atggcgttgc ctacatccaa
    25681 attttagaaa atacaaatca aatcaagaat tcattagcga cttttcgcac gattatgatt
    25741 ttgtgtatga ttgttttttg gattctctct attggtatca gttattactt gtctagtcta
    25801 agcatgcgcc ctattattct ttcttggaga agacaaaaag aatttgttga gaatgcttct
    25861 catgaattgc gcacaccgtt aacgattatt caaaatagtt tggaacatct ttttaccaag
    25921 cctaatcata ctattattga agaatccgaa agcatcgctc aagcattgtc agagacgagg
    25981 cgcctcactg gattgacttc ggatttgctg acaatcgctc ggaatgattc tgatcagcag
    26041 ctgctttcaa agcaaatgat caatacacaa gaatatatcg aacaacttgt taaaccattt
    26101 caagagatgg ccatcataga tggaaaagag tttattctcc ataacgatgc aacattagaa
    26161 gtaccaattg atgaaaaaaa aatccatcaa gttttggtta ttttgctgga taatgcatta
    26221 aaatatacga atccagaaga taagatcatt ttggattcat ctgttaacca taaagaatgg
    26281 ctgattcacg taaagaatac tggcccttct atcagtgaag aagatcaagc aaggattttt
    26341 gatcgatttt atcgggaaga tcaatctcgg gcgaaagaga ctggcggata cgggcttggc
    26401 ttggcaattg caaaacaggt aatcgaacag catcatggac agatttctgt atccaattat
    26461 cttccacaag gagtcgattt taaagtccaa ttgccgttga agcctaaaaa ataatgctta
    26521 tccctaaaaa agggctgtga caaaaatctt gtcacagccc ttttttgttg cgaataaacg
    26581 gtgttccaga agtagcttct tcggaaataa gccgaaattt ctttaagctg accacttctg
    26641 tcacaacctc tttagcttac gcttacgatt ttttattgcg gtcttcaatg tgtacatcac
    26701 cgcttgtgtc aatatccaat tcttctcggc taacatcttc tgtcactttt tcaacatctt
    26761 catgttcttc tttatgaatc ccaacttctt cacgaataac tgtatgtttg cttacatcta
    26821 cttgctcttc tttgattggg atagtaatcg tgtcatcttc caatttttcg tctgttccct
    26881 gtgaagattg atctgttact ggtttgcgtt cgatgacgat ttcttcacgt ttgaccggta
    26941 cttctaccgt ttcagtatcg ttgaccacat gtttgtggat gtcgacttct ccagttgtca
    27001 catcatgtgt atttacatct aatttttctt cttttagttg aattgtatca tcatctagat
    27061 ttggtttgcg atcgatgtct tcagtcttgc ttgtacccat tgtatcagtc tcgccaggta
    27121 cgtccatcgg atcagttcct ggtgtcatat tcaatggatc cgttgtattt tgaaagcctg
    27181 tcgtgccacc tacgtccatt ggatctgttc cagtgccagt ttctaatcca gtccccattg
    27241 tgcttgctgg gctaacagta tcttgtgtat ccattgtatc ttctgttgtt tctggctgac
    27301 gatagccttt tactgtgatg acaaattttc catcagaaat atctcctcta tattgatata
    27361 aaggatcatt ttctggtgta gccgtctcag aatcataggt atcaaaagaa aaagtatctt
    27421 ttattttttc ccataaagaa cggtcttctt caccatccat ccgattttca tctgtttcaa
    27481 catctatacc agacaatccg tcaaatccta gtctagatgc tgcttcatca gtagtataaa
    27541 taacgatatc atctcgttca tacccttcgt tacgcaaccg ttcaattact gataatgctt
    27601 cttccctcgt actatagctt ccgatgaccg ttaaattttt ctcttccatg taaattcctc
    27661 ctagcaaata gatttatgat tggtatattg ttacttataa atttattcta taggagacga
    27721 ttgttttcta caaatattta gattggaatt tgattaaaac aatttaattg aaatttgaaa
    27781 acatttatgt taatgaaagt catattctaa taaaatgtca tttgtttcta tatgaatttt
    27841 tatgaaaaat ttcatataat ttccactcgt tgtgatggtt taatttatcc aatccatcaa
    27901 taagcaaaca acttatataa aaaaggatta attctcaata taattgctcc taccatagaa
    27961 taaatcttca tgtaaagtaa tgaagtacca actatttcta atacttcttt aagaattatc
    28021 ccaacaatat cgttagacaa accattattg aataaatgga gagccaatcg attctgaaaa
    28081 aatgttttct aatttttcgg aaataaaaga aagagcctcc tgccattaga acgaaccaaa
    28141 tccatttcat tgctcatgaa gtaaccacat agctaaaaag aggataaatc aattaaaaga
    28201 tcaaaagaat gactgcacta ataaaataac taaaaatgta tcttcttact tcatatcttt
    28261 tagctagtac ttagttaaaa atttgcttcc taagatcaag acaaactgtc tatcttcatt
    28321 tgatgaaatg cggtaaaata tagaaaatga acactattgg agggaatttg taatggacag
    28381 caaaactaga aaacaactgc aagaaaagtt aaagcaacgt acacacttgc tcccttccgt
    28441 ctttcaacag tttttagaag aaaatcgttc ttccctttca ttaggcagtc gctatgaata
    28501 cgcaaaagat tttcatctgt ttactgatta tttgagaaaa gtattttctg aagaagtgga
    28561 tgatcagctg attatccatt tagaaaaaca agattatcag aatttcttag ctaaaatcta
    28621 tcgacatact cgttcgttcc aaactacagc gaaccaagaa acggagcaat tgtttgaaaa
    28681 tagctattac gggatttcaa ggaagcaata ttcattaaat cattttttac gatttttgta
    28741 tcaaaaagga ctgattgaag aagagatttc tcttttgggt acaagtttac cggaaacaac
    28801 caaagcagct ccgcgatata tcgatgcaga acttttcaaa gattttgagc atttgtttat
    28861 cccgattgaa ggctttcaaa caagcagaga agctagcttt gaagaaaaga atagaccgag
    28921 gaaccttgca attactgcca ttcttctgta ttgcggatta aagatccatg aagtagtaga
    28981 acttaaacgt aaagatgtat ctttgaccaa gcagattctt aatctgaata gaaaagaaaa
    29041 cagactgaat aaagtaccaa taccaaaaga agcgatgcca tttctggaga actattttaa
    29101 gttaacagca caaaatacgg acgaaaattc ttttgttttt cgttcctcac atgatcagca
    29161 aatttctcct cggactattc gccaaatttt gagcaaaatt acagtatata aaaacgtatc
    29221 gccagaacta tgcagaaaaa catatcgtta ttatgcagaa ctatatttag atgatgctga
    29281 cgcggtaaat tatttatgtg gtaatcgata cctctcatcc cattcttttc aagatatctg
    29341 ggaaaagatg gttgattttt cttatcacga attagcggca ttcgttcggg tttcttccat
    29401 ataaaacaaa tgtatgatct ttttttcaca ataaactatg ttattatata aaggattatg
    29461 ttacgattat cttgaatttg tatattactt aacaaaagat aatggagtgt gaatgatgga
    29521 gaccataacg tattcccctt tgaacttatt tacgaatttt caagaagctg cagctaactt
    29581 ttccgagacc ccgattatct ttgatgagcc actttcagct ttcccagaat taggacttga
    29641 aacaacttac aaaaaatgct gcgaaagtgt gttgaaacgg gcttatcaat tggctcatct
    29701 tggagtgaaa tccggagaca aagttattat ttataagagt tccgcttttg acacatacct
    29761 tctagctgtt tctgtgtctt accttggtgc agtgcctgtc atgacttctt atcatctacc
    29821 aacaacaaca atggaagtat ttatcgatcg tttagaagac cctttcattt tatttgatga
    29881 tgagacaaaa aagcgagtga gagaaatttc aaatggaaca aaatcaaaac agattcctat
    29941 tctttattta atggaacaac ctgctttgtc tgtttctcaa tcatttttag acaaaaatga
    30001 aatttcttat atgacccata cttctggtac aactggtatc cctaaactta tttgtcattc
    30061 tgctcattct atgggatggc ggacgaaatg gcaaaaaaca gttttcacaa aaatttcgga
    30121 aaagaaatta atcgcttttc acatctctcc agtgcattcc agattcaata ttggtatctc
    30181 ttctttgatg tccatgggat ttcctatgat gcctttagca aacgcacaaa gttcaaaagt
    30241 tgttcatatg ttggaagctc accggccaat tgcactagag acccatccga ataactttgt
    30301 tcagtggcgt tttactgcta aggagcatcc agaagctttt tcgggtatcc gatactatca
    30361 ctccactttt gatgctataa acaaccagac aatgaagaca tttttgcgaa cgtcgatggc
    30421 acaggatgcg gtatttcttc aagtatatgg ccaaagtgaa tgcggaccaa tgattcttaa
    30481 ggctcataca ctagagagtt tgaaaacaag tgatgcaaga gatatgggcg ttggactgga
    30541 agacatgaca agcgctcgga ttacagattc agttgggaat gtcctacctg aaaacacaga
    30601 tggtcatata caattactgt ctaaaggacg ggctttgact tattacaagg aagatgcacg
    30661 gttccaggaa aatgtttatg gcgattggtg ggatagcgga gattacggtt ttatggataa
    30721 gcatggccac ctatttttga aagatcggca agtggatttg atcgaaacga tcaatagtac
    30781 attagctatt gaagactttt tattagattc gttagatttt ttggcagaag tagtgattgt
    30841 aagggataaa aatcattctc cacaaccgat tattgcttta gctcctgata aagaaatgga
    30901 ttggaatcga tggtgggagc aagtccatga acttcctcgc ttgaacgtcc ctatcatccg
    30961 tgattttgat gcaatcccgc gaacagctac aatgaaagtg caacggcttc aaatcgagaa
    31021 agaattaaaa tcacaaaagt cctaactatt taaggaaaga aagatccatt ttggtctttc
    31081 ttttcttttt gtttttttaa attggatgag ataaaaatat gccaattggc tgtttttctt
    31141 ttttgaaaat ttcgttatac tcttattcat ggaggaaaat gttatgaaaa gaatcttgac
    31201 aattggcggt tctgatccat ttgctggtgg aggaatccaa acagatttaa aaacttttga
    31261 gaattttggt ctattcggac taagcgcctt gacttctata ggggcactag atattaatga
    31321 gcgatttatg ctggaatcta tatctttgga attgcttaaa cgacagttag tttcgattga
    31381 taaaatgact tcattagatg gtatcaaaat tgggttattg aattcaagtg aagcaatcat
    31441 ggttgttcga gacttcataa aaacaaaaca aaatatccca atcattttag accctgtttt
    31501 ggcattcaaa gaaactgaag ctgtgatcaa tcaattgtat atttcgcagc tcatcgaaga
    31561 actttttcca cttgttacga tgattacgcc taatttggca gaagctgctt tactttctgg
    31621 tcaagctctt ccctcttcgt taacagaaat gatccatctc gctaaagaac tcactgcttc
    31681 aggaacaaaa aacattgtga tcaaaggcgg cacaaggatg gctggagatg aagctgtgga
    31741 tcttctctat tcaattgatc attatgaggt attttctcat aaaaaaatcg acactacaac
    31801 agtaaacggg gccggttgtg ccttttcttc tgcaataaca gcgaatcttg tgttaggggc
    31861 agattttacc aaagcagtag cagaaagtaa aaaatttgtt gcccattgta tttctaatgg
    31921 cgtgctgatg aacgatcata ccggaagtgt ctggtctggc ggtattcaaa aaggaggaat
    31981 gagaaatgaa aactaagcaa ttaacgatct atgctatgct gattgcgttg acagttgccc
    32041 tttcattaac aattttgatc cctgtaccgg ctacgaatgg atttgtgaca ttatgcgagg
    32101 ccgggatcta taccgctgct tttctttatg gtccaacggg tggattagtt gttggtgctt
    32161 ccagtggtct attgatcgat ttgatttcag gatatccgca gtgggcagtt ttttcattag
    32221 tgatccacgg gttacaagga tatgttgctg gttctttcag caaatcttct actcgtatgt
    32281 ggggattagg gcttttatta ggaagcttag ttatgatcat cggctatggt atcgccggtt
    32341 ggattttata tgattggccg gctggagtgg cttctatacc aggaaacatc ttccaaaatc
    32401 tgattggaat gggacttgcc tttcctataa ctgcaagtct tcaacgtatt caagcacgga
    32461 agcaatatta attgaaaaag aaggttttag aaaatgaagt tgtcagaaaa agaattaaaa
    32521 gaccaattga ctgaaatagt taatgacata ttggctgagg cacacttgaa aaaaggtgat
    32581 ctttttgttt taggatgcag cacaagcgaa gtcgttggag gacatattgg aaaaaattcc
    32641 agtgcggaag ttggtcaatg gatcatccgt acgttaaaag agttgttgga tcctaaagaa
    32701 attgcgctag ctgtccaagg ttgtgaacat ttgaaccgag cattagtagt agaacgaaca
    32761 gtggcagaag caaaaaattt cgaaattgtc tctgttgttc ctgcactaca tgctggaggt
    32821 gcttgttctg ttgctgcttt tgaccagttc aatgatcctg tcgaagtgga acacgtagtt
    32881 ggacaagccg gtatcgatat cggtgacaca tcaatcggta tgcatatcaa gcatgtccaa
    32941 gtacctgtca gacctcgttt gaaaaccctt ggacaagctc acgtgacagc ccttcgctct
    33001 cgtcctaaat atatcggtgg tcctagagcg aattattgaa atgagtaatt gacagagcaa
    33061 tctgaaaaac ttcctcgcaa agtccaacta atgaacttta acgaggaagt tttacttgtt
    33121 tgttttatat aaccgagctt cataaggtgc tagtatgcct ttagcttcaa attctccgct
    33181 agaggaattt gacaagagta atgtccagga ctgatctgct tcttttggaa gttcatagtc
    33241 agcaagtgat tctgttaagt ttaccataat aagatattgt tcatcttcta aatatctttc
    33301 atagacataa agttgtggat gttcatgaag atattcttta taagaaccgt atatcagacc
    33361 tttattggtt tgtctaatgc ggatcatgtt cttatagaat tgcaggaccg actctggttc
    33421 atattcttgt tgttctacat tgatcgtttt ggtatttgga ttagtgatca gccaaggttc
    33481 atgtgtagaa aaaccagcat attgttccga agtccattgc atgggtgtcc tgctgttgtc
    33541 tctcgttgtt tcacgaatga tatccaatgc ttcttctctt gtcttacctt ccgcaagcag
    33601 ccttttataa agtcttttcg aatctactgc atcaacttga tcgatcgatt caaagggcat
    33661 attcgtcatt ccgatttcct gtccttgata aataaatggc gtaccttgca aaaagaaata
    33721 catcatagcg attgcttttg cagacatagc ccaaaaatcg ggacgagtat ctccaaacac
    33781 tgaaacggca cgaggaacat catggttttc catatataaa gcgttccatc cgataccatc
    33841 caatgaagtt tgccaagcgc ttaatgcatg tttcaattta aggacatcta attgcccttc
    33901 tttttcttgc tgccagatat ggatatgatc aaattcgaat atcatatcga aataaccgtc
    33961 tttccctacc cattgtggtc cttcttctgc agtgacacca cttgcttctc cgacagtcat
    34021 gatatcgtac tcttcaaaaa catgtttcaa atcggtcaaa tgttcttcga ttcctgagac
    34081 attttgaaag ggtgagaaag ggttctctgt ggcttttact gaatattcat ctttttttac
    34141 atgagaaatc gcatctaaac gaaatccatc tatcccttta tccaaccacc atcggatcat
    34201 attgaatagt tcttctttca atttctcgct ttcccaattt aagtctggtt gttcttttgc
    34261 aaaaacatgc agataatact gtccagtttc atgagaatac tcccaagcag aaccgccgaa
    34321 aatcgatgtc caattattcg gttctttgcc atctaccccg tcgacccaaa tgtaataatc
    34381 gcgatacggg ctttctttag attttttcga ttcttcaaac cattgatgct gatccgaact
    34441 atggttgatg acaagatcca taatcacttt gatccctaat tcatgtgctt cttttagcaa
    34501 taaatcaaaa tcttccatcg ttccgaatct ctcttctata tcttgatagt cacttacgtc
    34561 ataaccatta tcgacaaatg gagattgata gatcggattg atccagataa agccgatacc
    34621 aagatctttc aaataattaa gtttttcacg gatcccattg atgtctcctt gaccatcgcc
    34681 attgctatct ttgaaactag caggatagat ttgatagcct actgcttctt tccaccattt
    34741 ttgttcttgt atcatatcgt ttcactcctt taacggtccc tcttgattat aaccgattct
    34801 attttagaag tatccttgtt accggtaact agaaacgaaa agctcaacga atctgctaag
    34861 tgcgttcgct gagctttatt ttatcataga tagcgggcaa tcagttgaat catttgcttg
    34921 ggaaattcat tcaagtcagt aatatctaca aaacgacttt caccgtagat cgacttgatt
    34981 tcttctttgt cttggccaat agctgcggag acaaatagga ttccttgacg ttcatattct
    35041 ttcaaaacag actggatatc ttcttttgct tttttgcctg tataatccgg caacgctttt
    35101 ggctgaccgt cactaatatt aagcaacagt ttggttgttg cggattgtct gtttaatttt
    35161 tctgctgcta aacgaagtgc agcaccatca cgattgtttt gccttggttt catagtgact
    35221 aatttttcat ttaaccattg aaatccgtct tcaaattctt tataagaaaa caaagaagtc
    35281 ttttctcttg acgaacggtc tgctgtatct ccgtaaatca acaatgggat atcgactttc
    35341 ttagaaaatt cggcaatggc aatcgctgct ttttttgccg cttcgatccg atcatcccgt
    35401 atcattgacc cagattcatc gatccgtaca gctaccgcta aagaaggttg ctcatgtggc
    35461 gggttctttt tgtcaaaatt tctcaaatca ccatatgcta ctttgcttgc attgaaccgc
    35521 tgaccttcgt attttccttt ttggtacgtc tctgtttgtt cattatccag taactctaaa
    35581 atttgccggc taagtgattc tactattggc attacttgca tgtgtagctc acgtgcttcc
    35641 ggcgtttttc cttggaaagc tggtcggtga acgatgagac cttctttttc atgaatcgtt
    35701 tccttcatga tttgtcgcgc ttctttattc agttgcttct tgatttcctt atcatgtgct
    35761 tccatgtcag aagcagacat ttcttgagaa tcatctcctg tttgttgttc tttttgattt
    35821 tttccttcga tatcgctact gcgttctttt tgcatttgag gaggaatctc gtttttttct
    35881 attgtttctt cttgtttctc ttctccctca tttggttctt cagcagacaa cgatttcttt
    35941 tcatctaagc gcagaatttc ttcagcattt tcagtgatgt ttcgttcatt tgcttcttcg
    36001 gcgctaatgg ttctttcttt tgtatgctca gattgaggat gaccccattt ttcttttctc
    36061 aatatgtcct gaagttccag tagcaatcca ctgtcagaca aagcttgttc aagaattgcc
    36121 aattcatctg aatcagtgga taatttatac aaaacttttg gataaatcga ctggagtaaa
    36181 tcttgtcctt gcttataggt atttgcccaa taaaaataag aacgcattcc tgctacacct
    36241 ttgatggcgt tagcttttgc agtttcatcc aacaaacgga taatctctgc catctttaag
    36301 agaagttctt gtttttggat catagttttc gccatcgcac gttctgccat gacttctaag
    36361 gcaggaagat ccattttttc cgcatgttgc atccgatctc ttaaagattc atttaatggg
    36421 cgatttcctt ggtaatttcg atttgtagtg atcacaatga cgcaatctgg atggcgacga
    36481 atgattcctg tcggtaaatt caacatccca tttggttcga gcgctgagtt aagtgccaca
    36541 agaacagagg catctcggat gacagtcggc tcttgaatct ctaataaata gcctttttca
    36601 atcgctcgaa cgatttcaga agggtaatag ctgtaatgga ttgcatctgg ataatcagct
    36661 tcgatccttt gaactagctc tgctaatttt tctttagcag aaaaaggatc aatgccgtaa
    36721 tggttctgga tcagtgtcag tacagcttca agtgtatctg tttggtaaat agcttctagt
    36781 aattcttggt cttgttcctt attagaatct actacaggta ataaggctcc gaaaacatct
    36841 gatttgtcca tatctgcaaa acaggtcact tttgtatagg gaagttgcag atcagcagat
    36901 agtgctttgg ctaattgtgt cttccccgac ccggcatctc cttccaaaag tacgttactg
    36961 attttcattt caggatcttg ccaattccct ttgatttcgg aggcgatccg ccattcttct
    37021 gcacttgtcc gatggctttt cggtttcttc cagatcattt gcttttctag ccgtgaaaag
    37081 gttcggtttt ctgttagtga gaaattctcc atgcttctct tccccttttg atcatctctg
    37141 tctattaata tcctagctca tctaaaagac gttttaaaat acggaatcgt tccccaatca
    37201 gttctccgtc tttttgtagt gtatcgtaat acaaaatgat gtcttcatcg atctttggat
    37261 ttttggtatc tacttctacc gtcattcggt tcccttgtaa tccaatgatg tctacgactg
    37321 gattttctgg atcataaaat ttttcataac gataagcatc ttggaagtat aaacttcctt
    37381 gcgcagctaa gatcgctaaa tctaaaatac gtgtgattgg taattcttcc gactgtcgtg
    37441 accatttttc tccagtatgt cgccatacct tgcctgagat atcgactttt ccacgatcat
    37501 tccactgagc aaggcccagt gaaagccctt ttgcatccat atttaatgca tttcttccat
    37561 caactttatc atagtcttct acaacgatta ctggtttatg ttttaaattt gttggtattt
    37621 ccattatttt aactcctcat ccttttattt ttagtaaata agtaaattag tagtttactc
    37681 atttactaaa ctatatgcga cattatagag gattgaagtg aggattgcaa agaaaaagag
    37741 cttcattgat agaaataaaa acaccatctc gttcttttac acgctcatta gtaacatgta
    37801 gaaagtccaa gatgatgttg gaatattt
//
LOCUS       DAIGOK010000010        36513 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.010, whole genome shotgun sequence.
ACCESSION   DAIGOK010000010 DAIGOK010000000
VERSION     DAIGOK010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.010"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            679..1011
                     /locus_tag="NOA01_000377"
     CDS             679..1011
                     /locus_tag="NOA01_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HCI0631607.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1602..1931
                     /locus_tag="NOA01_000378"
     CDS             1602..1931
                     /locus_tag="NOA01_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631608.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            1918..2259
                     /locus_tag="NOA01_000379"
     CDS             1918..2259
                     /locus_tag="NOA01_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HCI0631609.1"
                     /translation="MKNYDPNSRWGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            2373..2612
                     /locus_tag="NOA01_000380"
     CDS             2373..2612
                     /locus_tag="NOA01_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631610.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            2679..2918
                     /locus_tag="NOA01_000381"
     CDS             2679..2918
                     /locus_tag="NOA01_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631611.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVMQYINDMQIT
                     GKEKACLLGSMKNFEQLRRTYVKKSSNCQLLLEVS"
     gene            3004..3675
                     /locus_tag="NOA01_000382"
     CDS             3004..3675
                     /locus_tag="NOA01_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HCI0631612.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            3728..5704
                     /locus_tag="NOA01_000383"
     CDS             3728..5704
                     /locus_tag="NOA01_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCI0631613.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAVKNAEGKYYGGLVDTDKDGVKEAVWVDNVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTEDIAFKVNKNSFKEENRITVKNNQKAAI
                     PLTGSNGFQTYVLISCLLLGAGGLSAVVYFKKKA"
     gene            5717..6472
                     /locus_tag="NOA01_000384"
     CDS             5717..6472
                     /locus_tag="NOA01_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCI0631614.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            6469..7293
                     /locus_tag="NOA01_000385"
     CDS             6469..7293
                     /locus_tag="NOA01_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631615.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQPVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            7595..9694
                     /locus_tag="NOA01_000386"
     CDS             7595..9694
                     /locus_tag="NOA01_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HCI0631616.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVLDGKNYDGALWYVEANGTWKKADTGGKLTVSSNTVTYTASDSFLANA
                     ANYGRDYILQLHMKAKNDAKGIAPNVATVTFNNDPNKGTQRTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIALDHYKQFSIQDPVEA
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(9732..11789)
                     /locus_tag="NOA01_000387"
     CDS             complement(9732..11789)
                     /locus_tag="NOA01_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HCI0631617.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNHEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKASQAQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(11903..12115)
                     /locus_tag="NOA01_000388"
     CDS             complement(11903..12115)
                     /locus_tag="NOA01_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631618.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            12302..12769
                     /locus_tag="NOA01_000389"
     CDS             12302..12769
                     /locus_tag="NOA01_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631619.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            12766..13008
                     /locus_tag="NOA01_000390"
     CDS             12766..13008
                     /locus_tag="NOA01_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HCI0631620.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            13026..13328
                     /locus_tag="NOA01_000391"
     CDS             13026..13328
                     /locus_tag="NOA01_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631621.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            13399..15453
                     /locus_tag="NOA01_000392"
     CDS             13399..15453
                     /locus_tag="NOA01_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631622.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGSLDYQISEGFYPTLIDLIR
                     LDIFKDTDETMSVEESYRNEKILQVLEEELESDQRKVNQVSEVEANEEVPWERPIFPK
                     DESKEKEKGIIDEQADILTHKSIFEEENEVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEDPTWTEGDSQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVE
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLRISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQAQIPKWKKEYEIHQQELKEQEEKQRKKEKEREQLELKKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLTHPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            15453..17285
                     /locus_tag="NOA01_000393"
     CDS             15453..17285
                     /locus_tag="NOA01_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HCI0631623.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNSVTCLA"
     gene            17998..19896
                     /gene="ltrA"
                     /locus_tag="NOA01_000394"
     CDS             17998..19896
                     /gene="ltrA"
                     /locus_tag="NOA01_000394"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCI0631624.1"
                     /translation="MTNTKGISLKNKQLRHAEYYDMTETFDELHAMSVAKSNFKNLMK
                     FIIDDNNIFLAYRNIKKNKGSATAGYDGVTIKDIEKLACGDFLTIIKRRFKDYRPRKV
                     KRVEIPKPNGKLRPLGIPSIWDRIVQQCILQILEPICEAKFNKHSYGFRPNCSTENAI
                     ADCLARINQSKMNYVVDIDIKGFFDEVNHTKLMRQIWTMGIRDKQLLVIIRKMLKAPI
                     VMRNGNVVFPTKGTPQGGILSTLLANICLNEFDWWIANQWEERTVKELKHHYRKNGAW
                     DKGNIYSKLRSHTSLKEMYLVRYADDFKIFCRTGEEAQRAFYATQKWLRERLKLPISM
                     EKSKVTNLNKAESEFLGFSIRLHKKGKKKVAFTYVCRKAEENIKNKLKQQIKKIQYAG
                     AKKNRHRAIGDYNMMVIGIHNYYRIASHCSVNFTEIYRSVRTVIEARLKARGMTKNGI
                     YGQDHEGYRPYLKSKQMRYIEGFPILPIGYIKKRTPLNKRRNLNKYTKEGRKLIHQEQ
                     QAIESWKIKYLVKGFSNNKKYTVEFSDNKISKFISQKGKCAITGNELLMEEIECHHIN
                     PYHLSKDDSYKNLVIISREIHRLIHATSEETILKLLRLLKLNHYQIRKLNTYRKKAAN
                     FPISIKMN"
     gene            20013..20813
                     /locus_tag="NOA01_000395"
                     /pseudo
     CDS             20013..20813
                     /locus_tag="NOA01_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
     gene            20810..23341
                     /locus_tag="NOA01_000396"
     CDS             20810..23341
                     /locus_tag="NOA01_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631625.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKDND
                     QSFVHQSPAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            23393..23725
                     /locus_tag="NOA01_000397"
     CDS             23393..23725
                     /locus_tag="NOA01_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631626.1"
                     /translation="MDKYSNMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            23726..24319
                     /locus_tag="NOA01_000398"
     CDS             23726..24319
                     /locus_tag="NOA01_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631627.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            24482..26176
                     /locus_tag="NOA01_000399"
     CDS             24482..26176
                     /locus_tag="NOA01_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCI0631628.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLLKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            26864..28741
                     /gene="ltrA"
                     /locus_tag="NOA01_000400"
     CDS             26864..28741
                     /gene="ltrA"
                     /locus_tag="NOA01_000400"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCI0631629.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            28872..29249
                     /locus_tag="NOA01_000401"
                     /pseudo
     CDS             28872..29249
                     /locus_tag="NOA01_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            29308..29571
                     /locus_tag="NOA01_000402"
     CDS             29308..29571
                     /locus_tag="NOA01_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system, antitoxin component,
                     AbrB family protein"
                     /protein_id="HCI0631630.1"
                     /translation="MLVKEIEMKKWGNSQGIRFGKEELSVLGETNTENLKFEMVVTEG
                     KIVLTPKNKKPETLDELFANYEGEPLGKEDKYEWGNMQGREII"
     gene            29571..29906
                     /locus_tag="NOA01_000403"
     CDS             29571..29906
                     /locus_tag="NOA01_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCI0631631.1"
                     /translation="MKKNYHDLSQGDLVYIDLNPTKGHEQRGKRPCIILSKPNKYLNY
                     MIGVAPITTKAKPFPVHVDLNGTQKIKGQILLEHHRMIDIESRGFQFVEAVPKEILEE
                     CIEKIKLFY"
     gene            29980..31182
                     /locus_tag="NOA01_000404"
     CDS             29980..31182
                     /locus_tag="NOA01_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCI0631632.1"
                     /translation="MENDTNIVMEHLKRRAKRRLFLWLFGTSAGLITILITVFVTLFL
                     ILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKL
                     LPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFP
                     NSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMSI
                     ITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGGE
                     FRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQ
                     EFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPL
                     PFLNSSKK"
     gene            31198..31842
                     /locus_tag="NOA01_000405"
     CDS             31198..31842
                     /locus_tag="NOA01_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631633.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            31853..32659
                     /locus_tag="NOA01_000406"
     CDS             31853..32659
                     /locus_tag="NOA01_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631634.1"
                     /translation="MSKKKGERQRFKKRKSYLQWLGIFMILGFLILASPIYTHQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKEKI"
     gene            32681..32911
                     /locus_tag="NOA01_000407"
     CDS             32681..32911
                     /locus_tag="NOA01_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631635.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYLAAKKYFTADNK"
     gene            33469..33747
                     /locus_tag="NOA01_000408"
     CDS             33469..33747
                     /locus_tag="NOA01_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631636.1"
                     /translation="MEVKIRDIDEKTIQRIDRRCIELSERTGTKWSRNRYLKVLIEND
                     FDHALLDYKKDQFDRLLEKFVDIQTYNTKILEEYIATNNQLIALLIEQ"
     gene            33786..34208
                     /locus_tag="NOA01_000409"
     CDS             33786..34208
                     /locus_tag="NOA01_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631637.1"
                     /translation="MAKATRKHIYLYDMHIEVLDEIISTHDDISNYSEAVRHLCMMYD
                     PKTEEKISPSQIKLNAIGKEVSILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            34228..35820
                     /locus_tag="NOA01_000410"
     CDS             34228..35820
                     /locus_tag="NOA01_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631638.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTLEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEYGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTEREKIEYQRLNQAMEKYQAEPHKKTSGTLPGVFSISSDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEVGLKAAGRK
                     MMETLFEKEKLNDTGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNSLTKVVKEPVRYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDENRTKLSPYTREKLDELTQYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QTQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            35850..36185
                     /locus_tag="NOA01_000411"
     CDS             35850..36185
                     /locus_tag="NOA01_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631639.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            36326..>36513
                     /locus_tag="NOA01_000412"
     CDS             36326..>36513
                     /locus_tag="NOA01_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631640.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT        13397 a         5587 c         7461 g        10068 t
ORIGIN      
        1 acgtagcctt ttacgtcata agtgttaaaa gaaataatta atttcaaata tagaaaaggg
       61 aacgtatgtt tgcatactcc ttttttttga gttataataa tacgcgaaga gagataattt
      121 cattatttaa acaagctaaa aatcttgata ttacttcttt tcaggcaata cgaatattat
      181 tcgcttacat tttttgtata aatttaattg tctgctatgc ttgtgatgaa ttgattttcg
      241 ttttgcactt ttggatgata aatttggaca attgaaaata caaaaaaaag aaaggcggta
      301 tgaactcgga aggctggtaa ccttcgtcag cgttcataca cggcattaag atttggccct
      361 taatgcgtta acctttcagt gttacggtat tacccgcaat ggatttagta taacagatat
      421 tcaaaagtga taccactttt tttggctgtg ttaaggttaa aaatacgtga tattacgatg
      481 aatctatgcc taagcataat gttataaaaa tgtccgtgtg gctctttgcc gtatgacgaa
      541 cgctgaaatt agagggaacg catgtttgga tgcgttccct cttttttgtt tgtcatgaac
      601 gaaaatcaat tctctatttt tgaaaaataa caagaaaaac ttgttaaaaa tatttaaaga
      661 aattgggtgg tttattttat gtcatataca ggaattttga gtctcaaaga tatttgtcac
      721 tacggtaaac ggtgtaccgc tacagagaaa atcacgaaga aactatcaac tggccaaagt
      781 aaaaccgttg tgcagtgtaa gaaatatata atccagaaag acaaagtctc agaggaaatg
      841 atttattaca ttgggaaaca aaagcaaata attttaaaag atcctatccc cttaaaagag
      901 ctatatccaa caatcaaaca tgtttatgac caaaacggtg tactgattgg tcgaagaaaa
      961 aatggagtct tgcgttgtac cgcaaaaggg atggggcgtt tgatcggctg aattgatcat
     1021 tctcataact tgtattttat cgtttttcgc ttgcttgaaa atattgtcga gacttctaaa
     1081 ggaacttaga gtcactagaa aaataaggag tagacttgat ctatcaacaa ttcccttcgg
     1141 ggagtgacgg ggtaggtttt tacgattggt aacgtaaaaa attggtcaat cgagtaaatg
     1201 cccttgtttg gatagtggtg aaatgaattt gtgtaaataa tatgcacaga gtaaacaaga
     1261 gggaatttag aagttagaca agttagaata gcgcctagat taatcatgag cagctactaa
     1321 gggatctagg ctttgacgca aactcatgga ataaggagta ggtcttctcg taatggggag
     1381 gacgcacaag aaattgtgtc gtgtgctgca gtacatgtag atacttgcga agacgttgat
     1441 ctttggatca atcgagcatt taccgttttc tactagggtt tctcgtttcg gacgccataa
     1501 acgagtcgat ttattttata tatttttgag tgaacgaaaa acgataaaaa tattcgttat
     1561 gaagtaaaac taatttagta gatagcaaag gtggtagagg aatgattcaa aaatttgcga
     1621 aaatgagtaa aactgcatgg attgaaaagc ttgaaaatga cttgagtgtc atgcgattag
     1681 ctccaaagaa attcaaacga gagattgcag aaaaagaagc aattttgtcg gtattgaatg
     1741 cggtagacga taaatgtcca gtaaaaatta gtttttcaga tatagcaaca attatcgaag
     1801 gtatttatta tggtgaagtt gatgaagaga tatcttctac aaaagattta ttagattttt
     1861 tggataaaaa caaagatacc tttatagtac taactgatta tgaggaggtt acaacaaatg
     1921 aaaaattatg atccgaatag ccgatgggga acacatacga ttaaggtttc tttccaacgt
     1981 tgggattaca aaggatttgt gacgtttaga agaggcggaa attgtaaagg attagatgtt
     2041 ttagcattag acgaggatga tttatacgat caaacattga ctgataatcc aattggattc
     2101 ggcttgctac ctgaagatga tgaaggtaat gaatggttta aaatgacgct tatgaatgat
     2161 aatggagacg aattatctgt tgaagatact tggtcatatt tgagtgatta tattgtcagc
     2221 gttgaaatca ttgaatttgt agcggataaa gaagaataaa aactggagga ataaatttta
     2281 cagataaagg cttagaagga gtcacgacga ttaataaaaa ggtagtatta ccttaggcat
     2341 tgagtaagga atttatttag gaggaaaatt agatggcgcg accagtttta gaggcgatgg
     2401 atatggatac tttgacaaaa ggaaggtatg tacagctgtt agctcgtggt tattcaccta
     2461 aagatgtatt caaatccctg tctaaaggaa cagatatgga aaaagaaaga ttacgaaaag
     2521 aattcgatta ttggagaacc cataatggaa tcaaggattt gaaaccagca cggccagtaa
     2581 ttaaacgaaa gaaaaagata acccaagact gaagtgtaga gcgtatgtga tcataggctt
     2641 ttttattttt gcaaaaaaca aggaaggtag gaaaactcat gaattatgaa gaaatcgaaa
     2701 acagaaaaaa agtgtcgaaa gaaatggaag aaaagttgct aaaaacgatg aaacaaaaac
     2761 accttaaaag attatctgtc atgcaatata tcaatgatat gcagataaca ggaaaagaga
     2821 aagcttgttt gttaggttca atgaaaaact ttgaacagtt gagacgaact tatgtgaaaa
     2881 aaagttctaa ttgtcaacta ttgttagaag tctcatgaca ttcattaata gaattcattt
     2941 ttaaaacttt ttctatggaa atgagaaaaa gattttttgt tttgtctgaa aggaggtagc
     3001 tggatgagaa aatggttatt tggtttttta ggagtcgctt taatcgtctt ttgcagtgtg
     3061 tttggctatg ttagctacca aaaacatgaa ggagaggtgt ttaagcaaaa tatagaaaaa
     3121 aagatgcccg tagatcaaat caatgcacat gcaaagagtt ataaggagga tgcaacgaac
     3181 gtcaataatg atatgtcttt ggggcaaatg ttgtccatac aaaaagaagc aatcgaaatg
     3241 ggcgtcaata aacaagtatt tgcacaaatt cagattcctg cacttggttt agcattgcca
     3301 atttttaaag gtgcaaatca atacactttg agtcttggag cggctactta tttttatgaa
     3361 gatgctgaaa tgggcaaagg aaactatgtc ctagcaggtc ataacatgga aatgccaggt
     3421 gttttatttt ctgatatcca aaagttgtca ttaggggagg tgatggactt agttagtaac
     3481 gatggcgtct atcgttacaa ggtaacgcgt aaatttatcg tacctgggta ctttaaatta
     3541 atcgatggcg ttcctgaaga gaactctttc ttgtctttgc caaagaaagg agagaaaccc
     3601 ttattaacgt tgtttacttg tgtttatacg tcacaaggaa aagaaagata cgtggtacag
     3661 ggagagctac aataacaagg ttttaatcaa cgcgagtcgt tgaaatatga aatgaggaga
     3721 gtaaaaaatg gcaataaatc ggtatgtaaa aaaagctgga atgcttgttg gaatgttagg
     3781 aatcattgca ggctgctttc aggggtatca agtacatgca gaagatgtca cgcaaaaaac
     3841 accaccagag aaggtcaata tcacggttca taaattaatg tacgatcaag gaacgcaatt
     3901 gaatgttgat attgacggga tcaaaaacga tggttatacg cacgatcaat atcctacagg
     3961 tgtaacgaaa tacaacaagg cagattatgg tgatgttgag tttacgttag gaaatattac
     4021 ggatcaagta ttaccgagag aagattctga tctaaccaac gcgaaagtgg acgagattgt
     4081 acaagatgta gaagacaagg gatctaacag tgagtacgtc aagaatgcaa ccaacaaaat
     4141 tacttcagct gtagatgaaa atggtgagat cacttttact gatcaaccag cctatgtcaa
     4201 ttccaaaggg aatgtatatg tggtttatga aagtaaatcc gctgctggat tagtcacaca
     4261 aaaagcaaaa ccaatggttg tgattgcgcc aatgacagat aaaacaggat caggattttt
     4321 aaaaaatatt catttgtatc ctaagaacat tgtttctaag ttgagttttg aattaacgaa
     4381 atttggtgac gatggcgcag accaaagcaa acaaaccctt ttaaaagggg caaaatttga
     4441 gttatacaag ggcgaacctg gaaaaggaac aaaattgggt gatcttgtat cggatgatca
     4501 aggaaagctg actgcgactg atttgacgtt aggaaaatac tattttgtgg aagttccttc
     4561 ggaagttgta gtgggatcag atcaagaacc aacagcagat caatatttat taggcgcaga
     4621 cgctcgcaac gatgcgcaca ataaattaac ctttgaaatt acgaatgatg gcgtgacaag
     4681 tgacttaaaa gcttcatacg taaactataa agcaccagtg atcgataaaa cagtaacaaa
     4741 tggaacagga caagaacatt ctttccaaat tggtgacgca gtaaattatc aaggaacgat
     4801 tcatattcca acagacattg caggtggcgc agacggtatc acagtaaacg gtgtgaaaag
     4861 cgaaacttct ccttacagtg tatttaaatg gggagataca gcaggacaag gactttctta
     4921 tgtcgctgct aaagcaaata tcaaagtaac aaataaagat ggatctgtgg tattaaaaga
     4981 aaatacagat tataaaatcc aaaattcaga aaatggcttt gtgatcgatt ttatcgtgaa
     5041 taatggccaa gtaagtgata ctgtagcaag tttacacgga caagatcttc aaatgactta
     5101 taacatgtat gtaaatgaca gcgcagctgt tgccaatcca ttaacaaata gcgtggattt
     5161 cgtttataac aacaatccat ttaatcagga agaacaccac gaaaaaacga aagctgacgt
     5221 tgtgacttat ggtgcaaaat tcttaaaagt cgatagcgga ctatttggaa caggaatcaa
     5281 agcaacaccg cttgaaggcg cagaatttgc tgttaaaaac gcagaaggaa aatattatgg
     5341 tggattagtt gatacggata aagacggtgt caaagaagca gtatgggtag acaatgtagc
     5401 aaatgcagcg attctaaaat cagataaaga agggcacttt gaaatcactg gtttaactga
     5461 aggtgaatat tcattggaag aaacaaaagc gcctgaaaac tatcaaaaat taacagaaga
     5521 tattgcattt aaagtaaaca aaaactcatt taaagaggaa aatcgaatca ctgttaaaaa
     5581 taatcaaaaa gcagctattc cattaacagg tagtaatggt ttccaaacgt atgtattgat
     5641 ctcttgcttg ttgctaggag cagggggact ttctgcagtt gtttacttta agaagaaagc
     5701 ctagaaggat cagctaatga ttattcgtaa gcccccaaaa agaagagtca tgcacaaact
     5761 gatgatattt ttttggttgc tgtcggcagt agcagtctat ttaattccag ttgtggaaac
     5821 aaatgtagcg aaatttcaac aacagcaaag aattttagcc attgaaaatc ttgaaaaaac
     5881 agattcttct ttggatgttg cgaaaataga gttaggcgat cctgttggtg ttcttacaat
     5941 tccaagtatt tctttaaaac taccaattta tgacggaact tccgacaaaa ttttagagaa
     6001 tggtgtaggg attacggaag gaactggcga tattacagga ggaaacggta aaaatccttt
     6061 aatcgcgggg cacagtggac tttataaaga taatcttttc gatgatttgc cttcagtaaa
     6121 aaaagggcaa aaattcttta ttaaggtaaa tgaagaacaa catgcctatc aaatttatcg
     6181 aattgaagaa gttaaagcag aagaattaca aaaaaattat ttagattatc ttatgcctga
     6241 aaaagaccaa gataggatca cgttaatgac atgcacacca aaaggtgtca actcacaccg
     6301 ctttttagtg tatggtgaac gagtttcctt cagtaaagaa gatttaaaac tacagaaaaa
     6361 agcaaaaaat aggaaatcaa tttcattgga ttggcgatta gcactgctat tcgctttttt
     6421 ttgcgttcct attattttta tttataaaaa aataaggagg agatcggaat gaataagaga
     6481 aaatggctgt ttgtttgtgt agcagctctc ttttctactg tagcttattc gcaactgtct
     6541 ttcgctgaca gcagtgatag tagtaccact caaacaatag ataattcaat tactgcacca
     6601 agcgaaacgg acagttcgga gaatactaca gaagaaacgg cagattcaaa tataagcttt
     6661 actgaatcaa cgaatccaac gtcagaaagt tcttcagaga cttcagaaaa gccgcaagac
     6721 gtagaagata cgcaagtgat caagcaagcc attcatattc aaaaaattat ttcttcgaat
     6781 caaatcaaca atcaaggcag tcaagtagcc cagcaagaag gtatcaatgg cgcaaagttt
     6841 attgtctacg atatcacgaa tatcttagaa gaaatgtcac aagatgataa tgagaaaaat
     6901 caacctgtag aaataattga gtcaaaattg aaagatcgag caaaaaaact ttcatacgac
     6961 cagttgaaaa aagtggctga aggagaaacc aaaacgattg atgatcagga aggcgtgatt
     7021 gaattttcca ttgaagtacc tgcaaacaaa aaacaagcat actatatcgt taatgaatca
     7081 agccctgaaa acatttcaaa cagtgaagat attgttttat tgacacctgt ttcaaatgaa
     7141 aaaggtgaat ttttgacgga tgtttggtta taccctaaat cagagaaatc agagccgaaa
     7201 gaagaaatta aaaaagttgt gtcaactggt gtaaagaaga acttttttga aaattgttgg
     7261 gaattttttg ctcaattatt ttttgataat taggatgaaa tgatgaaaat aataagaata
     7321 ttagttttta atctatcctt tttagccgca acattattgt gttgcggttt aattttttct
     7381 caattatcaa tttatccaag atatcaacga ttgttcattc attttctaaa cattcaatgt
     7441 gcttccatgc cattagaggt gattaaaaag ctcttgatca gtccaatttt tatggttagt
     7501 ttggttcttc ttacaggatt ggtaacgtgg gtattaggaa aaaaagaatg aaaagcttac
     7561 gatcgtttaa aaaaaatatt agatggaggg cattatggaa aataaaattc attgcactac
     7621 gggaagagta attactttag tgttgttttt gttccttctt ctttttgcga ggggaactgc
     7681 agtccaggcc ttagaaaaag ttgatccgag tcaaatcaaa gaaaataata atctggtgct
     7741 ttatcatgat agtgattcat tgagaaatat cgaagtaact gggatcaatg gatttaagat
     7801 actagatgag tattcaacta aaaaacaaaa agtacttgct tggccaaaag tcaaagattt
     7861 gaaaagtacg tcaaaaatcg ttgcccatta tcaaaaagta ggtacttatc taggaaaaga
     7921 aattgattgt gacgtgactt tttctaattt tgtagcgcac gctggggtta accaagacgg
     7981 attagctaaa acaattaatg gcaaaaagta ttataattat ttgctctttg ataaaaatat
     8041 ttatcgagga tttttcatgc agtcaatcaa tgtcatgaat gcaaaactag aatttaagta
     8101 tgcaggaaca aaaacaaaag tagctttgag tgaaccacag ccagaaattc aatcaaaaat
     8161 gagatcgatg agagcagctg gtattgattt aaacgataca tttctatcca tttatagttt
     8221 aaatggacag tatcagcaac gtgtgtcttg gtataatgac aataatgaat ctgtcggtga
     8281 acgtaatatt gatatgagag aatttgttgg gtatgaaaaa atgggtagct taccttattt
     8341 tgtcacaaca gatacagcat gtgcagaata caaagctcct gcgttatcaa caaacaattt
     8401 aacttcaaaa gtagtgggag gacgtgcaga aaaggcttat agctcgaatg atcatttcac
     8461 cgatgttgta ggagctgata cttatcacag aagtggtgta acgtatacgc ttcaaggcgc
     8521 ttccccaaca ttcatgattg gcgcaaatac gaatagtatg atgtttagct ttgatactgc
     8581 attgctatgg acaccacaac catcgaagcc tacaaaagaa gtgtttaaca aagctaatac
     8641 tgaagaggca gcacacaata ttgacaaaaa agtgattcca caaggatcag atgtttacta
     8701 tcatattcac caaaaatttg atgcattaac agtcaacaca atgaacaaat acaaatcatt
     8761 taaaatcact gatacctttg acagcaaaaa ttttgatatg gtattggatg ggaaaaacta
     8821 tgatggcgca ttgtggtatg ttgaagcgaa tggtacttgg aaaaaagctg atacaggagg
     8881 aaaattaact gtatcaagca atacagtcac ttatactgct tctgacagct ttctagctaa
     8941 cgctgcaaat tacggtagag attatatttt gcagctccat atgaaagcaa aaaatgatgc
     9001 aaaaggaatc gcacccaatg tggctactgt aacgtttaac aatgatccga ataaagggac
     9061 acaaagaaca aatacagtag aaaattattt ctctcttaaa ccaaataaaa aagtggaaca
     9121 agatgggaaa gatgttgatg gtcgaaatga tggtaaaaaa ggaacgccta cagctccttt
     9181 aaatgcagga tctgaagtac aatatctagt cacacaaaaa tggcatacaa aaggtgtaga
     9241 cattgcgctc gatcattaca agcaatttag tattcaagat ccagtggaag ctcgtttgac
     9301 gtataaagaa ggttctgcgc gagtgataga tatgagtaca ggaaaagata ttacttcaga
     9361 aggtacacta acctatgata gcaattcaag aacgctgaag tgggaagctt ccgctgattt
     9421 cttaagtaaa aatcttttag atggacgaga aattcaactg atatttacag ctaaaactcc
     9481 tttgaatgaa gaattggata tcgacaacca agcaaaagtt tcagtagaaa gtattgagaa
     9541 agcaactaat gtagtaacga ttggggtaaa acctaactta cctcagatca ttgtgccaaa
     9601 aactggatcg aatcatttag tgctaactgt tattggaagt atattatttc ttactttagc
     9661 atcattgggc tatgtagttt taaaactcaa ataaatataa aaacaaggct gagaatcagc
     9721 cttgttttct tttatttgaa gattggattc gcactaataa atacattatc tacgccaatt
     9781 ctaccagcag agtcaactac aaaattatga cctaatccga gaatactttg gttattataa
     9841 agtaaatttg cattatgggc agggctattt ttccatccta ccatggcatc gatagcacca
     9901 tcgtctgata aaaggtcatc aatatacagc ccagtttgac ttatgttttc tcccttagca
     9961 ccgtagcagc ctaagctttc caataaatct tggaaagtac caccaccagg gcgatagtga
    10021 ctgaagctga cttgtaattc tttagctcga atatctgcag cctgttgaac aatatcattg
    10081 aattgcattg gggcaacacc attttgttgg cgataatcat ttaaccaagc aagttctttt
    10141 tgacgcataa agttacgtaa ttcgtctaat gaaatctttt tatatttgaa tgtgattgtt
    10201 ttattttgat cgacgttatt aatggatcgt tggccgtttt tatccacggt aacggtagga
    10261 tcgtcatttc tgcacaagaa ataattttga tcagtcatgt ttttccaagg agtagcaaag
    10321 ctatcccctt cgtatactgt ttttgattca ctagagagaa cagcgttggt atcagcattt
    10381 acatgattaa cagtcacatt aaatttacga gtgtagttga aaatataatt ttgactttca
    10441 gtcacattgt ttaaggttac tgaatttgtt ggttctaccc gatcgtctaa aacatatcct
    10501 tgaatagaag gagcagttaa agtgacactg tcaccgtgag tgacattgac tatctgttct
    10561 ttcaaaatat taactcgtcc ttcgtttttg tagttgttat tgcacaacaa attgatttga
    10621 tgcacttgat tataattgaa tgtgattgtt gtattacctg tgattgtttt gctttgagta
    10681 tcattaccaa tcactttgta gctaaaatta tctttagcgc cttcgctata tttaaatgct
    10741 tttgccttag cagtgtaagt agaaccagaa gttacttttg cttgatctgt agcaatcact
    10801 tgacctgttt gattgtcaat atggttaata gtaaccgtat attcttttgg tgtttcaggt
    10861 ttgtgttgca accctttaaa tgcagcatca agactatttt tagcattatt tacttgggct
    10921 tgagaagcct tagaatcgtc tagtactttt ttagcattgg ataaagcagt ctgaaaatta
    10981 ttccaagatt cttctgtaaa atctccttta gcagttgatt ttactcggtt gtataaagct
    11041 tgtaattcag ctttattcac agtaggaact ggttttttct ttaatccttt gtaagcagaa
    11101 tctaatttat ttttggcact atccacttgt ttttgagtcg cttttgagtc atttaacact
    11161 ttcttagcat tgtttaaagc ggtttggaat gcgttccagg tattgtcggt atagtcacct
    11221 tttttagttc ccttaacgct cgtataaaga gcttctaaag ctgatttatt cactttgggt
    11281 ttagaggtgt cagcgccata ccaaccgata ccttcgtatt tccagccata gtctttggtt
    11341 aaccattgta cttctttcca acttgcagtg tagtgatgtg agccagattt tacaccagga
    11401 aggaaaaggc ggtagatcgg tttattattt ttttcggcag aacgccaggc tggcccttca
    11461 taagtccagc cgtaattttt agtcaaccat ttcacttcat cccaactagc tgtgtaatgg
    11521 tgatcacgaa gacctttatt gtacaaacga tagacaagat ggccaccgct agtcggtgct
    11581 tcccaggcgg ggccttcata atttccccat tttatcttta ctagatggtc acgttctttt
    11641 ttgctagagg tgtaaaaatg ttcatgatta tttggattgt aaaggcgata catgacgtta
    11701 gttgaagcag cgtcaacaga tccagcaaga ctaagtgagg cagcaaatgt tccaatagct
    11761 aaaacagttt ttggattaaa taatttcatt ttttcaactc ctaaatttaa ttaaaaatgt
    11821 ttacatgatt attatatatc ggctaaccga tatagtcaat aaaaaataag aaaagcctta
    11881 tataattttg gcttttctta ttttatttgt tagttgaagt attaatatca acgatttctt
    11941 cagccaattt atttaatttc atagcattta tgaatctcat gttgtctata ctagagcgtt
    12001 tgttggcaag gtcacttact gtagttaagg gtacgccaga ttctatggat atagaacgaa
    12061 ctgatttacc actatcaaac agcaaccatt cgattttctt tttatcagcg tacatttcaa
    12121 tgctccttct tgttttttct ttattatagc tcttttttgt aataaatcac aatatcaaga
    12181 gaaaaatttt ctatagaaag gtggattttt atgagtattc gttctccgtg tatccaaatg
    12241 aggtttgttg ctactttcgt tatttgatat actaaaaata aacaacgaaa gaaggaataa
    12301 aatgaagaaa aagcaactat ggtttgtctc tatgtttgca atatttttta ttattttaag
    12361 tggatgttca agtaataaga aatcggaagc taaagaagat aatagtttat tgcaaccagg
    12421 aactgtttgg aaagaagaag taggtggtct tattcataat ttaaaaatta tagatgatac
    12481 tacctgggaa tattctgaaa gtgtatggca tccagatcct gtacaaataa cagttaagag
    12541 gcaaaaagac tatgaaggtt tagaaagata caaaatagta gattctgcag gtattaggga
    12601 atttataaac aaacctgatt cattatttat tgttgttccc tatgagaagg atggcgtaaa
    12661 aaaaataatt tttcttggat ctagcaagga tgagaagcga acgaaagaga aattaattca
    12721 tgatggatca gagttaaata aatacaaatt acaaaagact agtgagtgat tgctagtctt
    12781 tttatttatc ctgaaggagg aatcacagtg gcaaaagtta aaacaccatt cgaaaaagcc
    12841 gcgtctaaaa tcttagaacg ccgtggtgtt tctgaaatac agtggaaaaa agaagttctt
    12901 gaaaaagcac aatttgacct attacgagga aaagataagg acttagaaga atatatctat
    12961 caacgtgaac gtgaaaaact tgtattcgat gaaattatta aacattaaaa ggagcgaata
    13021 atcttatgtt tgaatcaatt acaatgaatt ttttaactca accgaaagta gtagctgcag
    13081 ttgatatttt tagagcatta caaactgccg caactagttt gaaaaataat ctgatgattt
    13141 ttgctgctgt tgcggcagtg gcttccattg ctcttggagg tattttcttc ttctttgggc
    13201 gaagaggagc tgaaaacggg aagacacacg taggttatgt attgttgggg gtaggagtta
    13261 ttttagctgc cgcttctatt gtagtttcaa tgttctcaat gtttggacaa accccacaag
    13321 gactataaaa aaacaagtag aaggagtaaa aagtacgtgg ctacttttta ctcctttttt
    13381 tatggaagga gaatattcat gttttttacg atttcaggca atgcaaaatt ggataaagag
    13441 ttaataagaa aagcaacgaa gaaagtggga aagcgtactt cctttttacc gaaaacaatt
    13501 aaatattctg tcattgaaac ggaagaacag ctacatgtgt taggacaagt gttagaacaa
    13561 gagaatcaat cttctgttaa attgactctc acgttacatg ataaagaagg aaaaatagaa
    13621 aaggctggca cagaatacgt ttttggctca ctggattatc aaatttcaga aggtttttat
    13681 cctacattga ttgatttaat ccgcctagat atatttaaag atacggatga aaccatgagt
    13741 gtggaagagt cttatcgaaa cgaaaaaatt ttacaagttt tagaagaaga gttagaaagc
    13801 gaccaaagaa aagtgaatca agtgagtgaa gtcgaagcaa acgaagaagt accatgggaa
    13861 cgcccaatct ttccaaagga tgaatcaaaa gaaaaagaga aaggaattat tgacgaacaa
    13921 gctgacattt tgactcataa atcaatattt gaggaagaga acgaggtaat gggtcaattg
    13981 gaggtagatc cctttgaaga aacaatggag gatgaagaga cggccgaagt agaagttgat
    14041 ccagttgaag ctaatgaaga cccaacatgg actgaagggg attctcagga atatattgaa
    14101 ccacttatca gtgaagaagt agagaaaaat gaaacagaga tacaaaaggc aacggtattt
    14161 gattatattt tggatcaata tccgccagag catgaatgga tgaaagaaaa aaataaatta
    14221 tttttaaatg aactttattc gaaatatcaa ttacctcaaa cctatcaagc atttttagag
    14281 agtaaagaag aattaatctc acagggtaaa ggcacacttt tagaacagtt agagtctgtc
    14341 caatcaacaa attggcatga gcttgcacaa gaagatttat tagctacttt tgaagaacgt
    14401 caacaagaaa cagatcaaga aattaatgag tatgcgacgg aacagtcgtc gaattgggag
    14461 aaagcaaaag aggaactaaa taaagaagaa cagcgagtga ttgaagaaga agtacaaaaa
    14521 attaaagagc ggtacgaaaa gaaacgtgac aatgcgtatt ccttgtgtgt agaaaagatc
    14581 aacaatttta ctcagacgaa tcaagaagca ttatcgaaag aaaaagaaaa cttattaaaa
    14641 gaacgtataa aagaacgaaa agaagaattt taccgagggt tgcgtactga taagctaagg
    14701 attagcaaag aattaaatga acaactgaat aaactctatc aaactacgac tgaagttctg
    14761 atggaaaaac acgagcagat ccaacaagaa ttacaagcac aaattccaaa atggaaaaaa
    14821 gagtatgaaa ttcaccaaca agaattgaaa gaacaagaag aaaaacaacg gaaaaaagaa
    14881 aaagagcgcg aacaattaga actaaaaaaa cgtgaacagg atcttgcgga acaaaaaatg
    14941 gcgatagaaa aacaacggct agagcaagaa aaggaaaaag aagcagctgc tcgtaaggaa
    15001 cgtcaagaac aaatggatac attgcgacaa gcacagttaa attatctgac acatcctcaa
    15061 ccagtagctg ctgttcaaac gccagtgaac aaccaagcaa atttaactcc ttcaccccag
    15121 cgttccttta acggatggat ggtgggatgt attgcaagtt taagcttgct tctaggtgga
    15181 ggaacagtat ttgcttttaa tcatatcaat caatcttcac aggctgattc tgtagccaca
    15241 acggcgtcaa tcaaggaaga agcccaggaa caagcaagaa aagagtatga acaacaatta
    15301 gaagaattat tgaatcgtac caatcagtca acaacagagt caagcatgga aaatagcaaa
    15361 gagagtgatt ctagtaaggc taaagaagga tcaagcgatt caactgtaca atcaaacact
    15421 tccgaaagtt cagaatcaaa tgaggtgaaa taatgcgtgt tagagaaaca acgaagcgaa
    15481 aagacaaacg attcaattta gaaaagaata ggtatctttc tcatttctat cgctatactc
    15541 cctcaggaga agagctacca gaaaaacatg acaaacgcat ttcgaaaaag attttttata
    15601 gtggacttgt atacctccct ttagagtatt tagtaacaag agtaccagaa gttttcaacc
    15661 gattggaaat tgttaaggga agcttacaag gaataaatct gtttcaatta caggaaatgc
    15721 cacaggtctt tcattttgat tcagctggtt tttggaatcc atttactcct acaactagtt
    15781 tgggtacacg tttagcaatt tcgacagttt gtgtggttcc agctgtactt atgggattga
    15841 aaatggacat gtggtttcgt cctctagcag atggacaaaa aggggataat cgattaatga
    15901 ccttaaaaga aatccaacag atttatccgc aaatacctga aacaaaaact acttttccag
    15961 gttatggagg tattccaatc acacattaca aaaaatattg gtacattcaa acagatactg
    16021 ttaatacgtg tattgttggt acctctcgat cagggaaagg tcagattgaa gtgttagcca
    16081 ccattgacaa tttatctcgt gcagaaaaac aaagttcaat ggtcgttaat gatccaaaaa
    16141 cagaattata tgttggtagt aaagatgaac tagaagctag agggtatgat gtatatgcct
    16201 ttaatatcac cgatccactg caatcaatga gtgataatcc attggcactc atcgtgaaat
    16261 actggaaacg aggagacata gatactgcaa cacagttaac caatactttc acaaatacga
    16321 tttattatga tgccaacgct tcagataaca aatactttaa cgaaaacgcg cagaaagctg
    16381 tcaatgcgct tatttttgcg ttgttagaac aagctgatca aacaggagat tactccaaat
    16441 tgacaccgaa taatctcgtt gagcttcttt ctgagattgg cggttttaat tattatccag
    16501 atccagacaa tgaattcaaa cagttgaatg ccttagatga atttatgaat cagttgccac
    16561 ctggaaacat tgcaaaaaaa caatatgggg caacgaaaat tggatctgat aaagcgaagg
    16621 gaaatatttt atcaacggca atcacgggat tgaatccatt tactctaacg aaaattgcaa
    16681 agatgacttc tcaatcaacg attgattata aatcggttgg gtttccaaaa tacttagatt
    16741 tgaaattaca tgaaagtctt ttaaatcaac gagtcgtagt agaattttat cataagggaa
    16801 agaaatttca tgaagaacgt gtcaaagtag gctataaagg attttgtgaa atcaattttg
    16861 attgtcattt agttgcaggt gatcaagttt gtttaaaata cgatcatcaa ggaaaagaat
    16921 atgtggcttg gtatgaattt tctcaaagag tattgaaaga cgaagagggt aatatcgttt
    16981 atcggaaaaa acgaggagaa acccatttgc cagaattaga aaaagaagcc gttctaacat
    17041 tagatgagga gaaaagtaac ttattagtag agtctattcg gatgcactat accgataaac
    17101 caatggctat ttttatgctt acacctgatt atgatccatc taaccatatc attgttagta
    17161 tttttcttag tcagttatat aaagaattat ctacgcaatg tgttaagact aaaggagata
    17221 aatgtcaccg caggattcat tttattttag atgaatttgg gaatagtgtg acctgtttag
    17281 cctaaggata aaagaggcta gttccaacga acattggaat aactttacta tcgagaaatc
    17341 aaatgacggg gtaacgccct gaagggtttc cactgatact gcgacatgcg ttagtaggat
    17401 gtgcatccaa acgcgtatga agctcgctga agtcggcaga agggtaccgt aaggaaagac
    17461 acgatacgta tctaccacgc aagtgctcta gaggtagggt atgtacctga tatgtcgggt
    17521 gtcctaaaaa gttcaataag gtgagaatga tttgattatc tgacaaactt gcgaatggaa
    17581 tgtctgaaac gaataactca tagaaatgtg agggggaagg gggatacgtc ctctttccat
    17641 tgactcgagg ttgagtaaaa atgtgtttct atgaaaaacc ttgcatggtt aaaggctatg
    17701 gagtagtcaa caggcaaaag caagcaccta agttgaaatc tatggataga tagtaaggaa
    17761 caggggaagc atctagcacg gagggagcat accgaagatg tgtttctaag gaaaaaccga
    17821 aatcggttat aacttaggtg aatagatgtg aaagtagagc cacgaccagt gaaaatttcg
    17881 taatgaaatt ggaggaacag gctcaagtcg ttatttataa tataagaaac ctatttaaaa
    17941 tcatggattc tcgtaagact aaaaaggtca cgaaccagaa tggagcgtga taaaaccatg
    18001 actaacacaa aaggaattag cttaaagaac aaacaactca gacacgccga atactacgac
    18061 atgactgaga ctttcgatga attgcacgca atgagtgttg caaaatcaaa ttttaaaaat
    18121 ctcatgaaat tcattattga tgacaataat atctttctag cgtatagaaa tatcaaaaag
    18181 aataaaggaa gtgcaacggc cggatacgat ggtgtcacta taaaagacat tgaaaaattg
    18241 gcatgtggtg actttttaac tattattaaa aggcgattta aggattacag accaagaaaa
    18301 gttaaaagag tagagatccc gaaaccaaac ggaaaacttc gacctttagg aattccttca
    18361 atatgggaca ggatagtcca gcaatgtatt ctacaaattt tagaaccaat atgcgaagca
    18421 aaatttaata agcatagcta tggattcaga ccaaattgtt ctacagaaaa cgccatagca
    18481 gattgtcttg ctagaatcaa tcaaagtaag atgaattatg ttgtagatat tgatataaaa
    18541 ggattctttg acgaagtaaa ccataccaaa cttatgcgtc aaatatggac aatggggatt
    18601 cgagataaac aactccttgt aattattcgt aagatgttaa aggctccgat tgttatgcga
    18661 aatggcaatg ttgtttttcc aaccaagggt actccacaag gagggatttt aagtacactg
    18721 cttgcgaata tatgcctgaa tgaatttgat tggtggatag ctaatcaatg ggaggaacga
    18781 actgtcaaag agctgaaaca ccactatcga aaaaacggag catgggataa aggaaatatc
    18841 tattcgaaat taagaagcca cacaagttta aaagagatgt atctggtacg ctatgcagat
    18901 gattttaaaa ttttctgccg aacaggagaa gaagcccaaa gggcttttta tgccacacaa
    18961 aaatggctca gagaaagatt aaagctccca atttcaatgg agaaatctaa agttacgaac
    19021 ctaaataaag ctgaaagtga attcttgggt ttttcaataa gactccacaa aaagggaaaa
    19081 aagaaagtgg ctttcacgta cgtttgtcgc aaagcagaag aaaacattaa aaataaacta
    19141 aaacaacaga ttaaaaaaat tcaatacgct ggagcaaaaa aaaataggca ccgagcaata
    19201 ggtgactaca acatgatggt catcggtatc cacaattact atcggatagc ttcacattgt
    19261 agtgtcaatt tcactgagat ttatagaagt gttaggacag taatagaagc aagactgaaa
    19321 gctagaggaa tgacgaaaaa tggaatctat ggacaagatc atgaaggata ccgtccgtat
    19381 ctaaagtcta agcaaatgag atatattgaa ggttttccga tacttccaat tggctatatt
    19441 aaaaaaagaa ctccactcaa caagcggaga aatctaaata aatataccaa ggaagggaga
    19501 aagctcattc atcaagagca acaagctata gaaagctgga aaatcaagta tcttgtaaaa
    19561 ggttttagta acaataagaa atacacggtg gaattttctg ataacaaaat atctaagttt
    19621 atttcccaaa aagggaaatg cgctatcaca ggaaacgaac ttttgatgga agaaattgaa
    19681 tgccatcaca ttaacccata tcatttgtcc aaagacgata gttacaagaa ccttgtcatt
    19741 atctctagag aaatccatag attaatccat gcaactagtg aagaaactat tctgaaactg
    19801 cttcggttac tgaaactaaa tcactatcaa attagaaaac taaacacgta tcgaaaaaaa
    19861 gcagctaatt ttccaatatc aatcaaaatg aattagatag gaaacaaatt atattgtaat
    19921 taacgatgga acgccgtgtg cggtgaaagt cgcacgcacg gtgtggaccg ggggaaaaga
    19981 cggagataat atcaaactct tacctatcgg aattgccacc aatggataat atggaaggaa
    20041 tcatgacggt cacagctggt agaaacatgt tgtgggatct gtttattcaa tcttatcagc
    20101 aactttatgc gaagtatgga gaacaaaacg cagcgattat caaagaaaat tgtcagacac
    20161 atatttatat tatgtcgaca gatgataata ccatcgaaga attttctaaa aaagtgggga
    20221 ataagacggt tgaacaagag aacagtacca tcaattcatt aggtatgaat actcatatca
    20281 atcgcaatgt tgattcagat cgtatcttaa ctcctgaaag aatttctact tttttagaag
    20341 gtgaaacgat tgtactcgct cctttaaaac gacaagattt gaaaggtcat aaaatacggc
    20401 cgtatcctat ttttaatcat ggggaaacga atcttcctta tgcttatcaa tatattgaag
    20461 atttcaatcc atcgtgggat atcaatgata tagacataca ttgtacacat gcggacttgg
    20521 atcttaaggc tttacaaatt gattatgatt atttcctacc agatgaatat gctagaaatc
    20581 tctatcatgg actgcatgat caattagaag aaaaaaacga agaaaatatg ttttataaaa
    20641 tcgttcaatc tcttgagaat gagacggcta aaaaattgtt aattcaattt tacgaagaac
    20701 aagcagcaga tcgacttcaa gaatatgttg aaaagttgat caaaaataaa gaaatttctg
    20761 aaatgattgg gaaacggttg ttattggaat ctgaaaaggt ggtgaaagaa tgatcattgg
    20821 aggaattttt tctggcatag ccgactggtg gtcaggctta ttcggagaag gggatgcaaa
    20881 ccccaaaacc gtaacgaaag tcttacaaga ttatggtaat tatctgcagt atcaagatgt
    20941 atttagttgg atttggaaca cttttctgtg gggattgatt aaaggattat ataatctcaa
    21001 tactttacta gaaaaaacca tttatcaaag ctttgattta aaagacttat tgaacgccgc
    21061 tggggtaaat gcactatttc aaagtttgat tagtaaggta ttggctcttt tttgtgtcgt
    21121 cacattaatt tatttaggct tgaaattttt tatgagtaaa cacccaccaa aagtcaaaaa
    21181 tatcttggtt aatttattta tggccatgat cttgattttt ggtgggagtt ctttaatcga
    21241 tgaagggtta aatatcagta aaagctttta cggagacgtc actgcagcaa ataaagcaga
    21301 taaaacgggt tcacctgctt ttcaaattat tcaaaataat gtgatcgatg tcagtacatt
    21361 actagcaact gacccgaata aagtagaatc cctgccaaca gacaaaagaa atgctttaac
    21421 agcgaaaaat tttaaaacag cgaatatcaa tgggattatt actcctaaac aagctaaaga
    21481 aatggcggat aaagataaga gtggtttatc agatttacaa aaggatcggt taagagcgtt
    21541 acaataccga ttggaactag atgataatgg gaaagaaatt cctgttcaaa ttagtgacga
    21601 aggcttagca aaatatgtct atacttcagg gtatcgtcga tacagtagta aaccaggtat
    21661 tatcttagct ggtgaatctt ctttggcggt ggcctattta tttatcctat ttacgatcgt
    21721 tacttgcatt attgagctgg tatttaaaaa attctattta gtgattgccg cgtcaacgga
    21781 ctttgagaca ggccaacgga tgaaaagtgc gatagaagat atttctcaat ccttcttgtt
    21841 attggcgttt acgattttag aattacgaat ctatacatta atgctttcag gtatcggtga
    21901 tttgcatgcg gcaggaaaga tcaatggatt cttgtatgtt gttgctttaa ttgtgctaac
    21961 tattgcgcta ttcaaaggct ctcaatcggt tacgaagatt ttcggtgttg atacgagtct
    22021 gaaaaacggc agtaattctc ttcttagcat gtttgcctta ggaaatattg cgaaaaatgt
    22081 gggtagcgga gctaaaaacc ttgcaagtgc tggtaagagt ggtttaagcc atttaaatga
    22141 tattcgtaaa aatggatttg gccatcgaaa agatgttacc cctgataacg gggaagaaca
    22201 atcagacgat aaagctacaa ctgtacctaa tagtaagaaa acagtttccg aaatgttcgg
    22261 gaaagcgaaa aatggtgttt ctaaagccgc agaaggtctt ggttacgtca atgaacgtgg
    22321 gttaaaagga acagctgaag atcttacaga aaaagccaaa gacaccttag aagagaaatt
    22381 agacggcacg agagctaaag aattggctga agctgtgaag aatccatccg aagcactaga
    22441 aaaacaaaaa gaaaaagctt cagaagtcaa agaaggacta aaagatgcgt atggtgaagg
    22501 acaagttaat gcagcagtaa aagctaatac aacaaccgaa aaagaaaatc aagaaggtat
    22561 agtgattcct tcagtcaaag atcaccaaga agcgttgcac tctaatgaaa attcgattga
    22621 agagaaaaat tccatagaag aaagaaattc agaagaagga aaaaatggac agttaccaaa
    22681 aactgatcca gaacaatatc gctataaacc taacagtgac cttgaaaatg agaagcgaat
    22741 ttcagattta tcaaaagtaa atcaagaaga caaagcagaa aatgagggac agttaccaaa
    22801 aactgatcca gaacaatatc ggtatagatc tgttaactcc gaaaatgaaa aacgcatttc
    22861 agatttacca aaacgaaatc aagaagtaca aacggatgag gattccatcg ttctgccaaa
    22921 tgataatgga atcgaagttg gaactgaaaa atcaacgaaa attggtttac ctaaggcaca
    22981 aaatttacca atgaaagata gtcgtttgaa tcaattcaag gataatgatc aaagttttgt
    23041 tcatcaatca cctgcaggta ctgttcaaat gaaaccaagt cgtttgcctg aaaatggaaa
    23101 tctgacaact gtttcttaca atttatctga aagtggtgag acagggaatg cgtccgttca
    23161 aaatgttgtg acaagagaag ctgcaccaaa acctattcaa aacgttcata caggttacaa
    23221 gccagcaaat gtggcattta ctgcaccaac aaaaataaat gtgacaagag aaacaaaaaa
    23281 caggaacaat gcattgaaag aacaactatt gagggatatc aaacaggata tcaaaccata
    23341 aaaggcctac aagttaggcc ttttttctat gtatgaaaag gagaaatcat ttatggataa
    23401 atatagtaac atgaaaaacc ctccaagatt tagtggagaa tggaagtttt tcgctttttt
    23461 ctttttagat ctattaacgc ttggtggctg ctttttttgg gcgaaaatgg tcaataatct
    23521 tgtccactta ccactctttt ttgcactctt gaattatggc ttatggattg cgatagcgtt
    23581 agtgattgtt tggcgaccct ataataaccc aggtcaacga caaatttttg cgattatcga
    23641 gtctattttt tttgcggaca agaaccatta tcatgcgtta gatcctaacc gaaattatca
    23701 tggtacgtat aaggaggatg aataaatgcg agtcaaagga caaaattcag ttgaaaggaa
    23761 atccattttg cagtttggaa aaccagaaga gcgctctatc ttggacttat ttccgtataa
    23821 atcctgtaca gaagagggag tcttaattac tagagaagat cattttcaac gttattatcg
    23881 tgtagtttct acagacgtgg aaggattaaa tgaacaagag aaactagaac gaatgaatca
    23941 gttgaccacc gtcatgcgta cgtatgtgcc aacaattaag ttagttagtt tgaccacaga
    24001 gacggatcta acggaacaaa tcgtgcaaaa gcggcaatta ttagataaaa atcgtatgga
    24061 acagtcctta ggaagaaatc tacaacgatt aaaaaaatat gaaaagaaat tagtcgaaga
    24121 aatccaggaa ttgaaacgag cagaacaaga acgtcctgac ttaagcttct ttttcgtcat
    24181 tgaaggaaag acaatcaagg atttacaaac cagaaaccgt cagttaatgc gatctagtgg
    24241 cattttaggg ttaaaaccac tctacaaaaa agaattgatc aagattctgt atcgaatgaa
    24301 caatatgaat gatgagtagg aatggagaag agagaatgaa atatccgttg atatggttag
    24361 aaaaagatgt tccgaaggaa gagaccacta gaaaatttcc tcacacttta agcctgggta
    24421 agttgggaaa agggcaatct tttaaccaag taaagaatca aacaagaaga ggtgactgta
    24481 catgttagaa gcaattaaaa cagctttttc accacaaaat gatcaaaaag tacttgatcc
    24541 attaaagcaa ctaggaaatc gggaaatcga aattttaaaa tccaaagggt atgatttaga
    24601 ttttttaaaa caagtgatgc cacaaggtgg gttaaatttt gatgaagaat atgtgacgta
    24661 tggtaatggt tattcacgat gtgtgacagt ttatcactac acaaaaaatc ctacgctttt
    24721 ttggttggcc aatctcatga acaatcctca tacagtggcc acactcgatg ttagcacaga
    24781 tgaaaagcaa aaagtgattg atcaaatcaa tcgtgcctta gatgaattaa atgatcgatc
    24841 agataatgaa cgaaaagcga ctgaccgaaa taaaagcgcg gaagaatacc tggaactagc
    24901 ggaatatgct agaaaattga cttctaatgg ggaaatttct aagcaagtca aagtgagagt
    24961 acatgtttac ggaaacacga ttcaagaagt agatgaacga gcgcaggaac tgatcgaaaa
    25021 tctcaaaggg atggattatc gtgcggtgac ttatctgcat aaaaccaaac gagattggca
    25081 atcgatgttt accatgttcg atgaacaagc tgaatggttc ggaaatatca aagggcaatc
    25141 actaccatcg ttaaacattg gcggaggttt cccgttccac caccaaacgc taaaagatcc
    25201 aggagggaca caatatgggg aaaccatgac aggcggccca tttgtctgga atgcgacgta
    25261 taaaaatagt gtacggttgt ctactaatac cttgcttcta ggaatgatgg gagctggtaa
    25321 atcgaccgtt cttaaaatga tcgcggaagc ccatttagct gcaggagact ttatttgggg
    25381 ctttgaaaag ggaaaagact ttattccttt cttaaaggaa tataacggta ttatggtgcg
    25441 tttagacgga tctgacggaa tgatcaatcc tttagagata tttgccacac ggacgtatga
    25501 tgaagcatcc agtttatatg acgatggatc agtcaaagat ttgaagatca atgaagcagc
    25561 gtcctatcaa acacacttag acaaagtggt ctatcaggta cagttagtga gtcctcagct
    25621 aaaaggaacg atgaaagcag aatttaagac ctacttaaat cgtttttatg aagagtatgg
    25681 aacagtacca cgcggcttta cttcctcaaa ttcgcgttca aatactgata cgcaagtcac
    25741 agggaaagat ccagaagctt atccgacgtt caaagaattt ttagactttt taggacaact
    25801 ggaattacca ggtgcttccc aagagaaaaa gaatcgaaaa gaagaaatgg aatccatcgt
    25861 agaatcctta tgtgagacgt atggcatgat ttttgatggt cacagtacga ttcgacacct
    25921 tgaccaacaa cagttggtct tttttgacat tgactctatt acaggcttag atcgaggggt
    25981 acaacaatgt caaatgtaca tggctatgac gttgatttgg aatcaggcat tgattcaagg
    26041 aagaagacaa cgacgtttac ttaaagaaaa acaaatggaa gaacaagact tcaaacgctt
    26101 cttggcgatg attgatgagt gccataatgg tgcgacctgt tctgcagtga aaagctgtgg
    26161 gttgcaagta gtctaaaaac tattgtaata actcttataa cgagaagtca aatgagtggg
    26221 taacgccaca aagggcttcc actgatactc cgacatgcaa caagtagttt atgcagacca
    26281 acgttgtgtg aagctcggtg aagtcggctg aaggataccg taaggtaaaa gaaatagtga
    26341 ataccacgaa agttctttag aggtaaaggg tgtatccgat aagtcgggtg tccaaaaaaa
    26401 cttaatatgg tgagtatgta caaacgtact gacgaactag cgaatgtact gtccgaaagg
    26461 catagctcct agaaatggga gagacagtga gcaaaaaatc actcattgtg ctaacttgtg
    26521 gttgagtaaa agtcgttgtt atgaaagacc atgctgcgtt acaggcgtag gagaagttag
    26581 ggggcaaaag ctagcatcta agttaaggca tgtggataga tataagggaa caggggaagc
    26641 catcaacatg gagagtgcat actcgatgaa gtggtagtaa gaaatatgtg aatgacaaat
    26701 aacttacttt agtgatggtg aaagtagagt cacgactgat gaaggttctg taatggaact
    26761 ggaggaacag gctcgattcg tctgtaaaag tcatgaaacc cgttcaacca tgggattctc
    26821 gtatgactaa aaaggtcacg agccaattgg agcgtgataa accatgacaa aagaaacaag
    26881 tccaaaagat aggaagttgc ggcatgcaga atattatggc atgacggaaa tcttcgataa
    26941 gatatactcc aaaagtttga agggagataa tttcaagaat ctaatgcaga ttatcatttc
    27001 tgataacaac attgtcctag catatcgtaa tatcaagaga aatcacggta gtgtgacaag
    27061 aggagaagat ggagtaacga ttaaagatat tgaaaatctc aaccgtaatg aattcgtaga
    27121 aattgttagg aagcgattcg cacattataa ccctcggaaa gtaaggcgtg tagaaattcc
    27181 taaatataat ggtaaattaa gaccgttggg tattccatca atatgggata gaatcgcgca
    27241 gcaatgcatc ttacaagtat tagaaccaat atgtgaagca aaattcaata atcacagcta
    27301 tggtttccgc ccaaatagat cagcagaaca cgcaatagca gattgtatgg cccgaatgaa
    27361 tcaaagtcac atggaatatg tggtagatgt agatatcaaa ggtttcttcg acgaagtcaa
    27421 tcatactaaa ctcatgagac aaatctggtc tatgggtata cgagacaaac agcttctctt
    27481 aatcattagg aaaatgttaa gagcacccgt agtacttcca aatgggaagt tgatataccc
    27541 aacaaaagga acaccacaag gcggaatact tagcccatta cttgcgaaca tcaatctcaa
    27601 cgaatttgac tggtggatag caaatcagtg ggaggaaaaa gatgccaaag agctaaaaca
    27661 tcattttagg aaaaatggag cttgggataa aggtaatatc tatcacaagc tcagaaagag
    27721 tacaactcta aaagaaatat acctagtgag atacgcagat gattttaaaa ttttctgtag
    27781 aaattataat gaagcgaaaa aagttttcta tgcaactaaa atgtggttac aggagcgtct
    27841 aaagttacca atatctgaag aaaagtcaaa aatcacaaat ctttcaaaag aagaaagtga
    27901 atttttaggc tttacgatga aattagtgac caaaggtaac aaaaaggttg ttaatacgca
    27961 tataggtaca aaagcacaaa gcatgattaa agtgaaacta aaaaaacaaa tcaaacgaat
    28021 tcaaaaaagt cctaatagca aagatacctt tatctatatt ggagtctata acagtatggt
    28081 tattgggatt cataattact tcaaaattgc gactcattgt agtgtagcaa tgcgaaaaat
    28141 tgcaagagaa ctcagaccaa gttttgaaat acgactacga gaacaaggaa tctcaaaaga
    28201 aggacaatac ggaaagtacg aaggatacaa gccttaccta cggtcaggac aaatacgata
    28261 tataagaggg tttccaatat taccagtagg gtatattcaa cacagacatc cgatgtgtaa
    28321 aaaaagaaat attaataaat acaccgaaga aggtagggaa gccattcata agaagcaaca
    28381 aaccgtagaa gaatggaaga tatcgtggtt gtgtagcaat ccagtgctta gtaagagagc
    28441 aacgattgaa tttgcggaca atcgaatttc aaaattcatc gctcaaaagg gaagatgtgc
    28501 agtaacaggt gaggagctta tcttatctga aatgcattgt catcatatta tcccttatca
    28561 tgaaagcaaa agtgatagct atgaaaacct agtgattgtt actgaggagg ttcatcgggt
    28621 aatacacgct acacaaagtg aaacgattga ggaacttcta aaatatctaa aattaaatcc
    28681 aaaacaaaaa gaaaaactca atgagttgag attaaaagtt ggtaacgaag aaatatctta
    28741 ataaatattt gtaggacggg aaaacagaat gtcttttata gacgagggaa cgccgtatgc
    28801 ggtgaaagtc gcacgtacgg tgtggactgg gggaaaagac ggagataata tcaaactctt
    28861 acctatcagt atcgattaat ccagatttta ttgaaacggt aaattatgtc acaaactttc
    28921 aacgagaaat gcgtaaagta gaagcaatga ctgttttagc tacacaatcc ccacaagaaa
    28981 tggtgccaga aaatatgtct tctgataact tctcgaaact gaagaaagtc tttgaatttt
    29041 cttctacgaa gatctttatg cgtatggatg aatctatttt acgtcatata gaaggattag
    29101 ttggtaaatc aatgacgaac tcagaactag aatccattcc tcaacaaaat caaggagacg
    29161 ctgtgatcaa ttttggctca aaagaaacca ttcgtgttca ttttagtccg aacaaacgtc
    29221 aattgaaatt gtttgatggt ggaaagtaac ttcttgtatt ttggtgtata caagtatata
    29281 ctaatgtgtg agagaaggag ggattccatg ttagtaaaag aaatcgaaat gaaaaaatgg
    29341 ggaaattcac aaggtattcg ttttggaaaa gaagaattgt cagttctagg agaaactaat
    29401 actgaaaatt taaaattcga aatggtggta acagaaggaa aaattgtttt aactcctaaa
    29461 aataaaaagc cagaaacttt agatgaattg ttcgccaatt atgaaggaga acccttagga
    29521 aaagaagaca aatatgaatg gggaaatatg caaggtaggg agatcattta atgaaaaaga
    29581 actatcatga tttatcgcaa ggagatcttg tttatattga tttaaatcct accaaaggac
    29641 atgaacaacg gggaaaacga ccatgtatta ttttatctaa acctaataaa tatttgaact
    29701 atatgattgg tgtcgcaccg ataacaacaa aagcgaagcc ttttcctgta catgttgatt
    29761 tgaatggtac gcaaaaaata aaaggacaaa ttcttttgga acaccatcga atgattgaca
    29821 ttgagagtag aggttttcag tttgtggaag ctgtgccaaa ggaaatttta gaagaatgta
    29881 ttgaaaaaat taaattattt tattaaaagc agcgaagaat aattattatt tcttcgcttt
    29941 tttgttgttt aaaattaaaa agttaggagt actgactata tggaaaatga tacaaatata
    30001 gttatggaac atttgaagcg tcgtgcaaag cgacgcttat ttttgtggtt gtttggtact
    30061 tctgcaggtt tgattactat tttgatcact gtgttcgtaa ccctttttct aatacttgcg
    30121 gcgggttcga ttgataattc ggattccgat tcaagtagtg gtggcgaagc ttttacagga
    30181 gagtattcgg aaggcttacc gatttataaa gaaatcaaag gaagaggccc tttttctgat
    30241 gagattgcgc aatatgccgt aggtgcagca gtgaaatata aattattacc aagtgtgatt
    30301 ctttcgcaat acggttatga atcagcgttt ggtacgtcgg cgtccgcaag aaatgatctc
    30361 aactattttg ggattacttg gtttgatggt tgtctgtttc ctaaaggaac tgcacgagga
    30421 attggtggca ttgaaggggg ctggtacatg aagtttccca actcaaaagc tgcattctcc
    30481 tattacggct ttatggtagc cacacaatca aattttaatg catgtgtagg aaataaaagc
    30541 cctggcgcaa gcttattgat cttaggacga ggtggttatg ccgctgcagg aattactgaa
    30601 gatagtccgt attatacagg ttgtatgtca attatcactt ctaataaatt aaccgaatat
    30661 gatgaattcg ccatcaaaca ttggggtgaa ggtggcaata acaatggcac aattactggt
    30721 gaatggacga atccattccc tggcagcagt ttagataaaa gttccttcag cggtggtcaa
    30781 ttatttggaa cgaatccagg cggtgaattc cgtccgaacg gcttccatga tggattagat
    30841 tttggttcag ttgatcatcc aggaagtgag attcatgcgg ttcatggcgg aaaagtcgtg
    30901 tacgttggca atcctggtat ttcaggtttg ggcgcgtgtg tgatcgtgat taattacgat
    30961 ggcttgaata tggtgtatca agaatttgca aatagtacag gaaattctcg agtgaaagta
    31021 ggcgatcaag tgaaagttgg tcaagtaatt gggatacgag atacagcaca cttgcattta
    31081 ggatttactc gaatggattg gcgtcaagca caaggacatg cattcattga tgatggtaca
    31141 tggatcgatc cgttaccatt tttgaatagc agtaaaaaat agatcaggag aaaaaatatg
    31201 gaaaataaaa cgttaaaaat cgtcttaaca gctgcaatta gcgttagttt aatcggcaat
    31261 attgcgttgt tttggcagct gaaagaggaa caagcagcaa atacctataa aattgaagaa
    31321 gtggctaaaa acgcgtctga gaagctcaaa aaaaattcct ctgaggcaca ggcgaagatc
    31381 gacaagttaa atcaaaccat caatgagaaa aaccaagaaa tcgatcagct gaagcaacaa
    31441 gatggcacaa aagcaaaaga ggaagtaact gaagcacaaa gaaagacggc agaagagttc
    31501 ggtcaattgg ccattgataa atcattaagc agaaatgaat taacggaaaa actaaaagat
    31561 gtcgcaaccc aagaagtgat cgacaaatta agtcctacag acgatgatca ccaacatgat
    31621 gattacggaa gttactcctt tacattaggt gacgtgcaaa cttacgctca aacaagcagt
    31681 gtaaaagagg aacaaaattt tgtcgtattt gttgattata caatcggcaa tccgcaattc
    31741 aaagatgtca aaccacaaaa aataaaaggt ggattaactg tcactgaaaa acaagtagat
    31801 ggtcaatgga aagtcataga ctttagttat ttcactcgtt aggaggaaga aaatgagtaa
    31861 gaaaaaagga gaaagacaac gtttcaaaaa acgaaaaagt tatcttcaat ggctgggaat
    31921 ctttatgatt ctcggctttt tgattcttgc tagtccgatt tacacgcatc agaaggtaga
    31981 cataccagat gaagtaagca cgaacgcctt tttaaacacg aaggaactaa cgatggtttc
    32041 aaaagaaaaa ggagcgaacc agcaattagt ttttgaattt gcaatcgacg atgaagatca
    32101 aagcttatta aaagaactgg ctaatcttaa ttatcgcgta gaagtaaaga cagctaaagg
    32161 ggattatcaa gcaatacgag ccaaagtgat caaggtgtct gatgattatt ttgcagtgaa
    32221 gctaacgcat gtaccagaag aatatatcgc catgcgttta accattattc ctgaaaaaat
    32281 tgatccaaaa gtggatatgc aagaaccaca agatctgatt tattacgtgc acgaagataa
    32341 ggttaaagat caagtgaaag atgaagatta tgaacaacac gcgatcaatt acaaagtgaa
    32401 aggatacaag aaagaacaaa aagcagctca aaaacaaatg gaatctttcc aagcaaccat
    32461 tgatttgaat gaagaattag tgaagaagct aaaagatcaa ttgccttatc aagtcgcaga
    32521 tgatcaggaa aatactgaaa acaagattaa tagctaccag caagaaattg aaacatcgaa
    32581 aacgcaaatg aaagatcaag aagagatcat tcaaaaactt caagaaaaaa ttgatcgtat
    32641 gacaaaagaa aagatttaga aggacaggtg gagtgaaaaa atgaagcgaa caaacttggc
    32701 tgacaaagat actttcgctg agcagaaagt attgattaaa gcctatctca aagttgtaaa
    32761 agctaaagaa atatgtcgag aagtgaattt atctaataca acaatatctg agttgaagaa
    32821 tggtcgaaag aatttagata ctttaaaatt cagatcattt gaagctttgt atttagctgc
    32881 taaaaaatat tttacagctg ataacaaata aaaaacagga gtattttatg tgaaaaaagt
    32941 atataaaaaa tgcttatgaa tcagctatat caccttaacc atttgttaat gagatgggag
    33001 aaaacattaa ccacgattct aacgcaaaca tcaagcacat aaaacattta acactcctcc
    33061 tttctggctg aagatttcaa ccaaacagga agacagcgta tcaattatag catacacttt
    33121 ttcttaaatt catttttcat atgacaaaat atttccttct aatcgacaaa gttagaagga
    33181 ttttttattg tctaaataca caaataacct taatttataa agagcataag gaaacgtgta
    33241 cccactttca aaataaagtg ggtagatagg ctgtgcctat cacgtttctg aaaaaaataa
    33301 atgaccccaa aattttcaaa ttatgtgtag gtttatgggt agcaaaaata tgaatgtggg
    33361 tagcacaaaa gttcatttgt gggtagtaaa atttaaatat gtgtagcaat gtgtgtagct
    33421 acacacattg ctacacacgt tataacatat agtcaaaagg agatatttat ggaagtaaaa
    33481 ataagagata ttgatgaaaa aacgattcaa agaattgacc gaagatgtat tgaattaagt
    33541 gaacgtactg ggactaaatg gagccgaaat cgatacctaa aagttttgat cgaaaatgat
    33601 tttgatcacg ctcttctgga ttataaaaaa gatcaatttg atcggttgtt agaaaaattt
    33661 gtggatattc aaacttataa tacgaaaatt ttagaagaat atattgcaac aaataatcaa
    33721 ttaatcgcct tgttaattga acagtaaaaa taatattgtg aatttataaa aggaggaaat
    33781 caactttggc gaaagcaaca agaaaacata tttatcttta tgatatgcat atcgaagtac
    33841 ttgatgagat aatttctact catgacgata tatccaatta tagtgaagca gttagacatt
    33901 tgtgtatgat gtatgatccc aaaactgaag aaaagataag tcccagtcaa atcaaactaa
    33961 atgcaattgg aaaagaagtt tctattttaa ctgaaatggt ttcggagttt gcagatcttc
    34021 atttaaaaga tacaggaata ttaattggta caaattcaac tgtttatcaa gaagcgaaag
    34081 agcgtgtgga aggcaagata aaagcaaatc aaacgaagaa atattctcct aagataaaga
    34141 aaacaataag cgaatcagtg actacaaaaa gtgaagtatt tgatccaaca aggtttttaa
    34201 aacgataaaa attagaagga gaacgtgatg agtaaatcac ctgcagtcat tctcacttgt
    34261 caatttacct tgcccaataa aaaatctttt tcaacataca ttgattatat gacaagagaa
    34321 aaagcgctgg aagaaatgga acggcgcacg ctcgaagaag aacaggaact gcaacgaatc
    34381 aagaatgctt tagaggattt ttatattcct gaaggagaaa ctttttcgga gagtaagacg
    34441 gataaagaat tcagtgaaac gtcgaatgaa gcgcaatcgt tgatggaata tggaatcaat
    34501 tttgacaagt tagaggaaca agattttacc aaatatcttt cttatatgac gcgtcactat
    34561 gcgttagaac aaaaaaagga actaacggaa agggaaaaaa tagaatatca acgtttgaat
    34621 caagcaatgg aaaaatatca agcagaacca cacaaaaaaa cttcaggaac tcttcctgga
    34681 gttttttcta tatcttcaga cgaagtaaaa ggaagtgatc tgaaggagat taagcagatc
    34741 ttccaaagag ggcaacaaaa aggctcaatc atttatcaag atgttgtttc tcatgataat
    34801 tcgtatcttg aaaaaatagg attgtacgat cccaaaacag atactttaga tgaagttgga
    34861 ctaaaagctg ctggaaggaa aatgatggag acgttgtttg agaaagaaaa actgaatgat
    34921 acgggatatt ggatggctac cattcaccgt aacactaagc atattcatat tcattttgca
    34981 gttgttgaaa aagaaaatac gcgtaaacca tacactgagg tcgaaaatgg tgtgagttat
    35041 atcgtaccga aaggaaagag aagacaagca acattagacg caatgaagac caattatgtg
    35101 catgaattag aacgtttttc ttatgaaaag gatcgaatga taggcatgga gaaacgaaat
    35161 ttattaacgc ggaaaagtga tttgcgtaac agcttaacaa aagtggtaaa agagccagta
    35221 cgttatgatc gacacgcaat gaacttatta aatgaagtat ataaaacctt acctgacaaa
    35281 cgttcggaat ggaattatgg ggatgaaaat cgaactaaac tttcaccata cacaagagag
    35341 aaactcgacg agttaaccca atatgtgtta acaaaagaac ctgaatacca agaatatata
    35401 cagatttcaa aagcattgaa agaagaagca aaaagtcttt atggggaaag caagagagaa
    35461 tcaaaggatg cagagaaaaa tgctctcttt gatttaaaaa agcgcacagg aaatgccatc
    35521 ctaacagagc tgaagagaca ggatagtcaa ataaaaccat taatagaaag tgttgatcag
    35581 acgcaatcta tttacaatga acgatccttt cgaacgctta aacaattgcc aacgaatgaa
    35641 tacaaatata gttacgaaga attcatgaag cgcaggaaac aattccaaca aagagttgtc
    35701 acgcagcgga gtttgcgtca attaaatcga acgattaatg cccatgaaga aaaataccac
    35761 gcaagtaaag catacgaaga gcaacaacga agaatccaaa tggaacaaga aagacagtaa
    35821 agacagtaaa gacagtagga ggaatagcaa tgaaacaata tttagatcaa tggaaagtta
    35881 tagaaggatc attaagagaa gaacgaatcg aacaacttcc cgattgttta gaaaaagagc
    35941 atttgtttca gataagagaa atgttgataa acgaacagtt tgatccaaat caatttttag
    36001 tcgttgagta cccagctact ggtgtgtact gttgtaatca tgtgaagggt gaaaaatatt
    36061 ttatcattca agaatatgag ggcaagttag cgccttatta tactacttgg gaaatgaatg
    36121 aagaggggat caataacttc ccgtgtaaaa gcattgaaga gtctatttca ctcacagaat
    36181 gttagttgtt aagtgaataa aagaagtaaa agagtcctga acgtgattgt ccaggactct
    36241 tttttttcac tgaattgttt tccgccaatt cattgagtgg tgcgatttcc gtctacatta
    36301 tatcaaaaga aaggaggaag aagaaatggc ttattcaacg attggagttg ttgttgaaaa
    36361 aagcagagac aatcttgttt ttgtgacaga aattcaaaca ggcagagctt ttgtagtcac
    36421 agacaaagcg gcaaaagcat atcaaagtgg cgatatttta acgttaaatc tgactacgaa
    36481 gacgttcgtc gatgcggcag aagattatcc att
//
LOCUS       DAIGOK010000011        36024 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.011, whole genome shotgun sequence.
ACCESSION   DAIGOK010000011 DAIGOK010000000
VERSION     DAIGOK010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36024
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.011"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            101..1132
                     /gene="queA"
                     /locus_tag="NOA01_000413"
     CDS             101..1132
                     /gene="queA"
                     /locus_tag="NOA01_000413"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCI0631641.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            1751..4390
                     /locus_tag="NOA01_000414"
     CDS             1751..4390
                     /locus_tag="NOA01_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCI0631642.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(4558..5256)
                     /locus_tag="NOA01_000415"
     CDS             complement(4558..5256)
                     /locus_tag="NOA01_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HCI0631643.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            5474..6619
                     /gene="tgt"
                     /locus_tag="NOA01_000416"
     CDS             5474..6619
                     /gene="tgt"
                     /locus_tag="NOA01_000416"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCI0631644.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            6716..7096
                     /gene="yajC"
                     /locus_tag="NOA01_000417"
     CDS             6716..7096
                     /gene="yajC"
                     /locus_tag="NOA01_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCI0631645.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            7401..9998
                     /gene="adhE"
                     /locus_tag="NOA01_000418"
                     /gene_synonym="adhC"
     CDS             7401..9998
                     /gene="adhE"
                     /locus_tag="NOA01_000418"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCI0631646.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            10227..11333
                     /locus_tag="NOA01_000419"
     CDS             10227..11333
                     /locus_tag="NOA01_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HCI0631647.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            11350..12374
                     /locus_tag="NOA01_000420"
                     /pseudo
     CDS             11350..12374
                     /locus_tag="NOA01_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            12380..12859
                     /locus_tag="NOA01_000421"
     CDS             12380..12859
                     /locus_tag="NOA01_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCI0631648.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            12875..13654
                     /locus_tag="NOA01_000422"
     CDS             12875..13654
                     /locus_tag="NOA01_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCI0631649.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYKWVIRF"
     gene            13618..13941
                     /gene="kduI"
                     /locus_tag="NOA01_000423"
                     /pseudo
     CDS             13618..13941
                     /gene="kduI"
                     /locus_tag="NOA01_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            13961..14764
                     /locus_tag="NOA01_000424"
     CDS             13961..14764
                     /locus_tag="NOA01_000424"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HCI0631650.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(15013..16077)
                     /locus_tag="NOA01_000425"
     CDS             complement(15013..16077)
                     /locus_tag="NOA01_000425"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCI0631651.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(16074..17159)
                     /locus_tag="NOA01_000426"
     CDS             complement(16074..17159)
                     /locus_tag="NOA01_000426"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HCI0631652.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            complement(17172..18149)
                     /gene="mvaD"
                     /locus_tag="NOA01_000427"
     CDS             complement(17172..18149)
                     /gene="mvaD"
                     /locus_tag="NOA01_000427"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HCI0631653.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            complement(18142..19086)
                     /gene="mvk"
                     /locus_tag="NOA01_000428"
     CDS             complement(18142..19086)
                     /gene="mvk"
                     /locus_tag="NOA01_000428"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HCI0631654.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            19401..20060
                     /locus_tag="NOA01_000429"
     CDS             19401..20060
                     /locus_tag="NOA01_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCI0631655.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVS
                     I"
     gene            20145..21050
                     /locus_tag="NOA01_000430"
     CDS             20145..21050
                     /locus_tag="NOA01_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCI0631656.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(21052..21684)
                     /locus_tag="NOA01_000431"
     CDS             complement(21052..21684)
                     /locus_tag="NOA01_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCI0631657.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     misc_feature    21835..21960
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            22004..22528
                     /gene="infC"
                     /locus_tag="NOA01_000432"
     CDS             22004..22528
                     /gene="infC"
                     /locus_tag="NOA01_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCI0631658.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            22600..22800
                     /gene="rpmI"
                     /locus_tag="NOA01_000433"
     CDS             22600..22800
                     /gene="rpmI"
                     /locus_tag="NOA01_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCI0631659.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            22853..23212
                     /gene="rplT"
                     /locus_tag="NOA01_000434"
     CDS             22853..23212
                     /gene="rplT"
                     /locus_tag="NOA01_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCI0631660.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            23564..24802
                     /locus_tag="NOA01_000435"
     CDS             23564..24802
                     /locus_tag="NOA01_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HCI0631661.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLGISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAES
                     YTYNDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLL
                     PVLGVVLLDLPIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGF
                     LMTLAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFHGPMNLVGSGSA
                     ILAVVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIP
                     TGWIIGAILCLITFLLYGNA"
     gene            24850..26979
                     /locus_tag="NOA01_000436"
     CDS             24850..26979
                     /locus_tag="NOA01_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HCI0631662.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            26954..27643
                     /locus_tag="NOA01_000437"
     CDS             26954..27643
                     /locus_tag="NOA01_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631663.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFVTYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(28051..28737)
                     /locus_tag="NOA01_000438"
     CDS             complement(28051..28737)
                     /locus_tag="NOA01_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCI0631664.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(28873..29067)
                     /locus_tag="NOA01_000439"
     CDS             complement(28873..29067)
                     /locus_tag="NOA01_000439"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631665.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(29117..29557)
                     /locus_tag="NOA01_000440"
     CDS             complement(29117..29557)
                     /locus_tag="NOA01_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCI0631666.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(29561..30406)
                     /locus_tag="NOA01_000441"
     CDS             complement(29561..30406)
                     /locus_tag="NOA01_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HCI0631667.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLEKQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            30555..31973
                     /gene="pepV"
                     /locus_tag="NOA01_000442"
     CDS             30555..31973
                     /gene="pepV"
                     /locus_tag="NOA01_000442"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HCI0631668.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(32049..33251)
                     /locus_tag="NOA01_000443"
     CDS             complement(32049..33251)
                     /locus_tag="NOA01_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631669.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
     gene            complement(33238..35025)
                     /locus_tag="NOA01_000444"
     CDS             complement(33238..35025)
                     /locus_tag="NOA01_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631670.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            complement(35038..35991)
                     /locus_tag="NOA01_000445"
     CDS             complement(35038..35991)
                     /locus_tag="NOA01_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCI0631671.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
BASE COUNT        11412 a         6637 c         7602 g        10373 t
ORIGIN      
        1 ttaggaaaac aaaaaataga agtgaaaaag tgctgatttt gccaacagaa cgaaaaacat
       61 gatagaatga tgaagcacat gatagaaagt gagtgacgat atgttaacaa cagaagattt
      121 tgattttgac ttgccggaag aattaatcgc acaaacacca ttaaaagacc gcgatcattc
      181 tcgtttactt gttttggatc ggaaaactgg agagatggaa gataaacatt tccatgaaat
      241 cattgaagag ttacagcctg gtgatgcgct agttatgaat aatacacgag ttctgcctgc
      301 tcgtttatat ggagaaaaac cagatactgg tgcgcattta gaagtccttc ttctaacgaa
      361 tacacaagga gatacgtggg agacattgat caaacctgcg aaacgtgcca aagttgggac
      421 aagaatctct tttggtgatg gcagattgca agcagtagtg aaagaagaat tggaacacgg
      481 cggacggatc attgactttg agtatgaagg aatttttcta gagattcttg aatcattggg
      541 agaaatgcca ttgcctccat atatcaaaga acgtctggaa gacccagatc gctatcaaac
      601 tgtctatgcg aaagaaaatg gttctgctgc agcgccaaca gctggattgc attttacgaa
      661 agaattactt gatgagatcg aagcaaaggg tgttgaatta gtctatctga cactacatgt
      721 agggttaggt acattccgac ctgtcagcgt agataaaatt gaagaacacc atatgcatag
      781 tgaattctac cgtttgacgg aagaagcggc tgagcgattg aaccaagtac gtaaaaatgg
      841 cgggaaaatc gtggctgtcg gaacaacttc aattcgaaca ttagaaacga ttggaacgaa
      901 atttaatgga gaaatcaaag cagacagcgg atggactgat attttcatta cgcctggcta
      961 tgaattcaaa gtggtagaag ctttctcaac taatttccat ttgccaaaat cgactttagt
     1021 catgttagtt agtgcctttg caggaagaga attgacttta tctgcttacc agcatgcgat
     1081 tgaagagcgc tatcgtttct ttagttttgg agatgcgatg ttcgtgaaat aaggttatga
     1141 aaagccgcta gttttgaata acaaaaaagc tttacaaaga ttttatgaca aagaaaaagc
     1201 cgagctctcg ataagctcgg ctttcttgta cctcggaagg attcaaataa acagaaatga
     1261 ttgtaacttt ccatcataca tgcaaagcac ggtgttgaat ttcttcatgt taaacaaaaa
     1321 ttttgttttc gttacatctt caacatttga aacatctctt gttcctattc aattttcaga
     1381 cgtttacgtt attcaattgt tacgtatcgc attacttctt gtttcggata cttcgtgatc
     1441 caaacatcca tacgcattca ctatttatag gtttgtactc tacacgcatg tctgggtgaa
     1501 acctgtactt ctaacaactt tgtttgctta cactgttcta tcgagaaatt cttctgtcta
     1561 tttgatggta tcctccttcc ttacatctat ataatagcat cctttggtag cgtttgcaaa
     1621 tataagcact ttaaatttta cgctttctta acaaataatc gataagccct ttatttgaag
     1681 taaataaaaa aaggatgtta tagtaaaata agtacctttt tttgtacgaa gattttaaag
     1741 gaggaatagg atggaagaat acaagcagac aatagaagaa gtaaagaagg taacagaagc
     1801 agatccagat actgggttgt cttcacaaga agtggatgaa cggcgaaaaa agcagggatt
     1861 gaataaattc gatgaggcgc cgaaagaatc gatgatcaaa aaattctttc gaagcttgtc
     1921 cgattttaca acgatcatcc ttcttgtagc agcggtgatt tccttttata ccgcatttgc
     1981 tacagaacat ggggatttat ttgaaggact gctgatcatc gctatcgttg tgatcaactc
     2041 ggtattggcg attgtccaag aaggaaacgc agaaaaagca ctggaatcct tgcaagatat
     2101 gaataaacaa acggcaactg tcatacgaga cggcaaagtg accactgttg agtcagagca
     2161 actggtagtg ggtgatattc ttgttttaga gtccggtgat gctatttctg ccgatgcccg
     2221 tttgatcgaa gcatcacaat tacgtgtaga agaatcggcg ttgactggag aaagtgaagc
     2281 agttgaaaaa gatgcagcat ttattgctga agaagatgag tcattaggtg accaacttaa
     2341 tatggtattt aaaggatgta cggttgctgc aggtcgtggg aaagcgatcg tcactgccat
     2401 tggcatggct acagaaatgg gtaagatcgc ggatcttttg aacgaaaata ctatgcaaaa
     2461 aacaccttta caggtccgat tgaatcagct aggcaaacga atcagtatga tcgctttagc
     2521 ggctgcggct ttagtatttg tgattggtga actgcaagga gaaccactcc ttgagatgtt
     2581 catgacttcg atttccttgg ctgttgcagc tgtaccggaa accttgactg ttattgttac
     2641 gttgacactt gcatacggtg tgcagaagat ggcgaggaaa catgcgatca tccgacagtt
     2701 gccagcagtt gaaacgctag gaaccgctaa tgttatctgc tcagacaaaa caggaacgct
     2761 gacacaaaat gaaatgcgcg tccgccgggt atggagcaaa gaagatgaag taacgaatat
     2821 cgaagattca atgacgaaca gcgcgatgga aatattgaaa atggctgcac tatgtacaga
     2881 tgtgacggtc gagcaagaag atgacgacct tgttatcaaa ggtaatccaa cagaagtagc
     2941 catcgtaagg gctgttgaag agaattatca tacaaaggca gaattagaag aaaaatatcc
     3001 acgagtaaac gagcttcctt ttgattctga acgaaaaatg atgacaacag ttcaccaaat
     3061 gggcgaaaaa tacatctcaa tcaccaaagg agcattcgat gttcttgctc cgcgctttag
     3121 ttcaggcgat gtagaacaag cggcgatcgt taatgatcgc tttggtaaac gggcactgag
     3181 agtgattgct gttggttatg ctacttatga cgaagaaccg caagatattt cttctgaagc
     3241 actggaaaaa gacttacgtt tgattggtct gatcggaatg atcgatccac cacgtccaga
     3301 aagtaaagga gcaatcaagc gagcgaaaaa ggctggcatc aaaacagtca tgatcactgg
     3361 agatcatgtg gtgactgcca gtgccatagc gaaagaatta ggtatcttga atgatccgtc
     3421 agaagcactt tcaggatcag aactgcatca attatcagac gaagaattag atgcgcgtgt
     3481 caaagctctc tctgtctatg ctcgggtaac gccagaagac aagatccgga tcgtcaaatc
     3541 ttggcaacgt acgggtgcag tcgtagcgat gactggggat ggtgtgaatg atgcaccagc
     3601 gttgaaagca agtaacgttg ggtgtgccat ggggatcaca ggaacagatg tggcgcaaag
     3661 tgccgcagat atgattttga cggatgacaa ctttgcgacg atcgtagacg ctgtcagtca
     3721 aggacgttcg gtttatcaga atattcgtaa agctatcaac tttttgctaa gctgtaatat
     3781 ttcggagatc tttattgttt tgattgcgat gctgttagga tggggagcac catttacagc
     3841 tgtgcaacta ctgtttgtca atgtagttgc tgatggactt ccaggatttg ctttaggaag
     3901 agagccagcg gaacacggca tcatggatca gccacccatt ccgaaaaatg aagggatctt
     3961 tgctcgaggt ttattgcaaa aaattgccat aaatgcaggg gtcttcacca tcgtaacctt
     4021 gtttggttat tatttaggta gttatgtcga tactatcagc ccatgggtag acgcaagtca
     4081 gcatgtcggt caaactgttg cattcttgat cttagcttat tcatcgattc tgcatgtgtt
     4141 caatgtacgc agcagccaat cgatcttcaa agtcaattta gcgaccaata aagctttggt
     4201 agaaatggcg ttactggcat tagctattac aacggctgtt gctttgctgc catttacgca
     4261 agaactattc ggcctagtac acatcagttt gaatcactgg ttcttggtag ggattttatc
     4321 tatcgtacca atcgcggtga atgaactgat caagttccat cgattaccgg aagcagaaga
     4381 agaggaatga ttaactgttt ctaagttgat tttcttttta ctggaactat acaaaaagga
     4441 ggtgtgacat gaatgttgtc acacctcctt tttgctaatg ggctgaatgt ttcttttact
     4501 gcctctcgat aaacggtgtt ctcctgccac cccctcggtc acaagtcaca ctctctttta
     4561 aatatctttt ggcaattgtt cataaaatgc tgcttttgtt gcagaaatgt atgggctgag
     4621 ccatagataa ccaataccag cagttgctaa tgctaagata tgccagccga taaatgaaag
     4681 atctaaccag aaaagttgtc ctttataacc atccatcaat tttcgacttg cagtgatcgt
     4741 atccagtact ttaggtgttt gtcctgtttc ttggacgata tcgtagtaaa taaaatagct
     4801 ttgagaataa gaatagcttt taatgattcc aggaataact aatagcaaac cccataatgt
     4861 tgtaaagatc gagatcagca cagctaagct gatcactccg ccgataaaca aacctttgaa
     4921 tccacgaaat gcatctttga atggttcgat ctgatctttt tgtccacgta aaatatcaag
     4981 ataagtccaa gaaataccgc tcatgaacaa agcgctaatg atgctggaaa taattccgcc
     5041 accgcctcct gaactaccgc ctcctgaagt tgtcgcttct gagacgctcg ctgatccatc
     5101 atatatggtg gtggcaaaca agataatagg aatcataata agaagaatag tagcaatacc
     5161 aatcaatgta gggattagat ttagtaaaac agcttgtccc caacgtcctt ttagactctc
     5221 ttttgcttgg cgttttaatt ctgcagaaga ttgcatgatt tttccctcct tttctttaat
     5281 aacaccaata taccatgtat caaaaaatat ttctgtcata atgtttttct tttttttgtt
     5341 attcataaaa aaaggataaa aaaaagaata ttaaataaag tagaaggaag ccgagggaac
     5401 ttgcaaaaag cacttgaaac gattatgatg gtgggagttg tcaagatatt ttatatagaa
     5461 agtagggaaa tgaatgacgc aacctgccat tcgctatcgt ttaatcaaga aagaaaaaca
     5521 tactggcgcg cgtttagggg aattgatcac gccacacgga actttcccca caccaatgtt
     5581 catgccagta ggaacattag ctacagtaaa aacaatgtcc ccagaagagt tgaaagaaat
     5641 gggtgctggc gttattttaa gtaatacata tcatttatgg ctacgccctg gtgaagactt
     5701 agtagaagaa gctggcggac tgcataaatt tatgaactgg gatcaaccga ttttaacaga
     5761 ttcaggcggc tttcaagttt tttcattaag tgatatgcgg aatatcgaag aagaaggcgt
     5821 gcatttcaaa aatcacttga acggttcgcg tatgttttta tcaccagaaa aagcaatcaa
     5881 catccaaaat aaattgggct ctgatatcat gatgagtttt gacgaatgtc caccattcga
     5941 tgaaagttac gactacgtga aaaaatcagt agaacgtacg tctcgatggg cagaacgagg
     6001 attgaaagct catgctaatc cagaccgtca aggattattt ggaatcatcc aaggagcagg
     6061 gtttgaagat cttcgtcgtc aaagcgcgaa agatctagtc gggatggatt tccctggtta
     6121 ctcaatcggc gggttatctg tcggggaacc taaatcagaa atgaatcgtg tattagaatt
     6181 cactacaccg ttgatccccg aaaacaaacc acgttacttg atgggtgtcg gtgctgctga
     6241 ttcattgatc gatggtgtta tccgtggagt agacatgttt gactgtgtct tgcctactcg
     6301 aatcgctcga aatggcacat gtatgacttc tcaaggccgt ctagtagtga aaaatgcgaa
     6361 atatgcccat gatttccgtc caattgatga gaaatgtgat tgttatacat gtaaaaacta
     6421 tacacgtgcc tatatccgcc atttgatcaa atgtgatgaa acatttggga ttcgtttgac
     6481 ttcttaccac aatttatact tcttgttgaa tctaatgaaa caagtacgtc aagcaatcat
     6541 ggatgataat ttgcttgaat ttagagaagc cttttttgaa gaatacgggt tcaataaaga
     6601 aaacgcaaaa agcttctaaa ggaaaatctt ttaaaaatta agtaaaaaac tagtgttatc
     6661 agcatttttc tgctaaagtg ttttttgtag atacagaatt agtgaggtga aaaaaatggg
     6721 aacattacca atgcttgtga tgtttatcgc gctattagca atgtggtttt ttatgtcacg
     6781 ttcgcaaaag aaacaacagc aagaacgtca aaacttgtta gatagcatga aaccaggtga
     6841 ttcagtcgta acgatcggcg gattacatgg agttgtttca gaaatcgaca atgacaaacg
     6901 gacagttgtt ttagactgcg aagggatctt ccttgagtac gatcgtgcag caatcaaaac
     6961 ggtcaaacca gggactgtcg taacaaatga tgcaacgact actgttgaaa caacagaaga
     7021 accagctgag acagttgaag taaaaactga aacaacagaa acgctggaag agacagaaga
     7081 gaaggaagaa aaataagtat caaagcttat ttaaagacga ggtagggcag aaagatgtcc
     7141 tacctcattt tttattcaaa aaagaaagag ataagagcat gggatatatt gctcaatatt
     7201 gaataatcgg gtgtgtcata taaataataa taaataactt tatatatttt tctatacaaa
     7261 cacctgtaaa taaagcaatt ttatatttaa aaataactgt gaaaaaaatc acaaaaaagt
     7321 gtttacatag aaaaataaat attgtatgat atacatgtga attatttaac aaataaccat
     7381 gacccattag gaggataatg atggctaaag taatagaaaa agaaaaaacg aagacaattg
     7441 acgtacaagc aatgattgat gatttggcag aaaaagcaaa tgcagcattg aaagaaatgg
     7501 aaagctttga tcaagaaaaa gtagaccata tcgtgcatga aatggcaatg gcagctttgg
     7561 atcaacatat gccattagcg aagatggctg tagaggaaac aggccgcggt gtttacgaag
     7621 ataaagcaat caaaaatatg tatgcgtccg aatatatttg gcataatatc aaacatgata
     7681 aaacagttgg tgtgatcaat gaagatgtcc aaaaagggtt gattgaaatt gctgaaccag
     7741 tcggtgtggt ttgcggagta actccaacaa cgaatccgac ttccacaacg attttcaaat
     7801 caatgatcgc gttgaaaaca agaaatccaa tcgtttttgc tttccatcca agtgcccaaa
     7861 aatcttctgc agaagcagca cgagtggtac gtgatgctgc tatcgctgcc ggagctcctg
     7921 aaaattgtat ccaatggatc gaacatcctt cgatcgaagc gacttctatg ctaatgaacc
     7981 atccaggtat cgcgatcgtc ttagcgacag gtggagctgg aatggtcaag tctgcttact
     8041 ctacaggaaa accagcttta ggtgtaggtc caggtaacgt accagcttat attgaaaaaa
     8101 cagcaaaaat taaacgtgca gtgaacgatt tgatcgtttc gaaaacattt gataatggaa
     8161 tgatttgtgc ttctgaacaa gctgtgatcg tggataaaga aatctatgca gcagtaaaag
     8221 cagaattcca agcacaccaa gtttatatcg taaaaccaga tgaattgcaa aaattagaag
     8281 atgcagtgat gaacgaagga aaatatgcgg tcaatccatc aatcgttgga cattctgcga
     8341 tggaaatcgc aaaattggca ggaatcagtg taccaaaagg aacgaaaatg ctgattgctg
     8401 aattggaagg cgtaggccca gattatccgc tatcaagaga aaaactttct ccagttcttg
     8461 ccatgatcaa agctaacaac acggaccatg cgtttgattt gtgtgaaggt atgttgaatc
     8521 ttggaggatt aggacataca gcagttatcc attcagaaaa cgaagagttg catgtcaaat
     8581 tcggtcttcg aatgaaagct tgtcgtattt tagtgaatac accatctgca gaaggcggca
     8641 tcggtgatat ctataacgaa atgattccgt cattgacttt gggatgcggt tcttatggta
     8701 agaactctgt ttctcgtaac gtctctgctg taaacttaat caatgtcaaa actgtagcga
     8761 aacggagaaa taacatgcaa tggttcaaat taccgccaaa aatcttcttt gaaaagaact
     8821 ctctattgta cttagagaaa atggaaaacg ttgaacgtgt catgatcgtt tgtgaccctg
     8881 gtatggtcca attcggttac tgcgatacag ttcgtgaagt attggcacga cgtaaaaatg
     8941 atgtgaagat cgaagtcttc tcagatgttg aaccgaaccc atcgacaaat accgtatacg
     9001 caggaacgaa actgatggca gacttcaagc cagatacagt aatcgctctt ggaggcggtt
     9061 ctgcaatgga cgcagctaag ggaatgtgga tgttctatga acacccagac acatctttct
     9121 ttggtgcaaa acaaaaattc ttggatatcc gtaaacggac atataagatc gacaaaccag
     9181 aaaaaacaca attcgtatgt atcccgacaa catcagggac tggttctgaa gtaacgccat
     9241 ttgcggtcat cactgacagt gaaacacatg tgaaatatcc gttagcagat tatgcattga
     9301 cgccagatgt cgcaatcgtc gatcctcaat tcgtcatgtc agttccagca tctgtcacag
     9361 cagataccgg gatggatgtg ttaacacatg caatcgaatc ctatgtttca gtcatggctt
     9421 cagattatac acgtggattg agcctacaag caatcaagtt agtctttgac tatttagaaa
     9481 aatcagtaaa aacacctgat atggaatcac gtgaaaaaat gcacaacgct tctacaatgg
     9541 ctggtatggc gtttgctaac gcattcctag gtatttgtca ctcagtcgca cataaaatcg
     9601 gcggggaata cgggatccct catggacgga caaatgcgat tcttttaccg catattatcc
     9661 gctataatgc aaaagatcca caaaaacatg cgatgttccc taaatatgac tatttccgtg
     9721 cagatacaga ttacgcagat atcgcaaaat tcttaggtct gaaaggcgaa acaacagagg
     9781 aattagtcga agctttagca acggctgttt atgaattagg aaaatctgtc ggcatcaaca
     9841 tgagtctaaa agctcaagga gtcacacaag aaacactaga tacaaccgta gaccgtatgg
     9901 ctgaattagc ttacgaagac caatgtacga cagctaatcc gaaagaacca ttgatctctg
     9961 aattgaaaca gatcatcatt gatgcttaca acggataagc aaaagaaagc gtagaaaaaa
    10021 gataagaaaa tagatgaaaa agaacttgga aaacgttgtt tctccaagtt ctttttttaa
    10081 tttccaagta actgaaagtt acttttgtta tttaacattt aggtcaattt tagacagttt
    10141 ttgttcaaaa agcgatattt atagtggacg gtattactgc tatattaagg atataaaatg
    10201 aaagcgttta aaaaggtggc gtaaagatga ttattaaaga agagattttt gtacctaaag
    10261 aattagtgaa agaaaaaatt gacctgctcg ttgaaaacct gacaacgatc aaagacgata
    10321 atggtgaatt tttgttggac tttgatggac tgaaagtaga tgacaaaagc tggacagtgt
    10381 ggaattggcc acaaggtgtc ggtctatatg gtatttataa aaattaccag atgacaaaaa
    10441 atcctcgcgc ttatcaagtg gtcaatgaat ggtttgagga tcgtatggaa gaaggcgctc
    10501 cgcccaaaaa cgtcaataca atggcaccgc tattgactat ggcttacttg tatgaagaca
    10561 cgaaagattc acgctatttg ccttatttag aacaatgggc agaatgggtc atgaatgaca
    10621 tgcctcggac aaatgaagac gggttacagc atgccacata tggaccggaa aataagaatc
    10681 agttgtggga cgatacattg atgatgacag tgcttccttt ggcaaaaatc gggaaactgt
    10741 tgaatcgccc agaatattta gaagaagctc gttaccagtt tatgatccat atcaaatact
    10801 tgatggataa acgaacagga ttgtggtatc acggctggac gtttgaagga aatcataact
    10861 atgccgaagc attttgggct cgtggaaact gctggttgac gattgctatt ccagagatca
    10921 ttgaaatctt agaattatcg aaagaagatg cattgcgcaa attattgatc gaaacacttg
    10981 aagcacaagt gcgtgcatta aaaacttatc agtctgaaag cggtttatgg catacgttgt
    11041 tggatgatcc ttcttcttac gtagaatctt ctgcaactgc aggatttgct tacggtattt
    11101 tgaaagcggt ccataaacgt tatttaccac aagaatataa agaagtcgct tatcgtgcta
    11161 tccaaggatt gcttgaacaa atcgatgaaa aaggagaagt tcaaaacgtt tcaatcggga
    11221 caggaatggg ggacagttta gatttttatc ggaatatcgg aatcacagca atgccttatg
    11281 gacagtcgtt aacggtgctt tgtttgacag aattgctcgt ctcttattgt taaaagaaag
    11341 gaaacccata tgaaaaaaac atcactgctg tttgcagcaa gttcgcttag cctaggtctg
    11401 ctaggtctaa gcggctgcgg aaataattct agtgtggcaa ctgtcgcaga tgcagacgta
    11461 aatgagatag ttaccttgcg ctttgcttat gcaagcaaca gccagtaatt gatgcaatga
    11521 atgaatttgg gcgtttagta aaagaaaaaa cagatggttc cgtacagatc cagtatttcc
    11581 cagatggaca actgggtgga gaccgtgaac tgatcgaatt gacacagtca ggagcagtag
    11641 atttcacaaa agtaagtgcc tcagcgttgg aaagtttctc taaagattat tcgatttttt
    11701 ccgttcctta tctattcaat gatgaagaac atttctttaa ggtgatggat aatgaagaaa
    11761 taatggagcc aatctaccaa tcgaccaaaa atttaggatt taccggattg acctattatg
    11821 attctggtca gcgcagcttt tatatggtag acggtccgat ccatacgccg gaagatttga
    11881 agggaaagaa gatccgtgtg atgcaaagcg aaaccgctat caaaatggta gagcttttag
    11941 gcggttcgcc cgtacctatg ggaagtgatg aagtgtacac gtctcttcaa tccaatttga
    12001 tcaacggatc agaaaacaat gaatttgtct tgtatacagc tggacatggc ggggtagcta
    12061 aatactattc ctatgatgag catacacgtg tgccagatat catcatcatg aatgatgcca
    12121 tcaaagaaca gctaactgat gaacaggaaa aagccattga agaagcagcg aaagaatcaa
    12181 cggtttttga aattgaagct tttgcaaaag caattgaaga agaaaaagct ttagcacaag
    12241 aagaacatgg agtccaattc aatgatgtag ataatacacc gtttcgagag gcagttgacc
    12301 cacttcatga tatattcaaa aacgatccta cctacaaaga cctttatcaa aagatccaag
    12361 agcaaggagc gtagctgtaa tgaaaatgat tttaaacaag gtattagaga tcctgggagc
    12421 tgttttgctt gttgggatgg tcgctgttgt tttatggcaa gtattttcca gaacggtttt
    12481 aggaaatcca aatacggtca cggaagagct tgttcgattc ggtcttgtct ggtttgccat
    12541 gttgtcttcc gcgtatgttg tgggacaaaa agcacattta gccgtaacgg ttttaagtga
    12601 aaaattaact ggcaaaaaaa gtaaaatttt agaagttgtt gttcaaagtt tatttctggt
    12661 atttgctgcg atgatcatgg tgtatggcgg atggaatgct gtcacattga caatggggca
    12721 gatctcgcct tctttaagta ttcctatggg atatgtttat ctgtcagtac ctgtatcggg
    12781 ggtactcatc attatctata gcctgatcaa tctaatcgat acattaagca acaagccagc
    12841 aattcaagaa aaaatataaa aatggaggaa taaaatggca atagaagccg gattagtgtt
    12901 attttttgta tttgttgtgt tgcttgtatt agggatgccg attgctataa gtattgcatt
    12961 ctcatcgatg atgactttat tgttagttat accgtttgat gtttctgctt ttagttcagc
    13021 tcaaaaaatg gtgggaagta tcaatagttt ttcgctgatc gctattccgt ttttcgttct
    13081 ctcgggcatt atcatgaata atggagggat agctaagaaa ctagtggatt ttgcgatgtt
    13141 gttcgtcggg cgcgtgccgg gggctttggc tcatacgaat gtgttaggga atgctttatt
    13201 tggttccatc tctagctcgg ccattgccgc ttctaccgcg attggtggag tgttgatccc
    13261 tcagcaagtg gaagaaggat atgaccggaa gtttgcgaca gcggtcaata tcgcgtcagc
    13321 tccgacaggt atggtcattc ctccaagtac tgcttttatc atgtattcac ttgttgctgg
    13381 tggtgcttcg atctctgctt tgtttatggg aggttatctc gtaggcgctt tgtgggcgct
    13441 aggaatcatg ctagtagcat tcatcgttgc taaaaagaat aaataccctg ttgtcgaaaa
    13501 aggacagatg gctcatgctg gaaaaataat aaaagaggcg attcctagcc ttctattgat
    13561 tgtcatcatt atcggtggta tcttgacggg gatatttaca gcagtagaag cttcagcgat
    13621 agctgtcatt tacaaatggg ttatacgatt ttagaaccag gaagtgcttg gaatacgatg
    13681 ccttgtcata cacatgaacg tcgtatggaa gcttatgtct attttgatta tgccaatgaa
    13741 gatacacgtg tgttccatat gatgggcaaa ccagatgaaa caaaacattt agtcgtagac
    13801 aatgaacaag ccattatttc gccaagctgg tcgatccatt caggtgtagg tacaagcaat
    13861 tactcattca tctgggcaat gtgcggagaa aatatcacct atacagacat ggatatggtg
    13921 caaatggatc aattaaaata agcataaggg agagaaaatg atggaattta gtatggatat
    13981 gtttcgttta gatggaaaag tagcattagt caccggtgct gtctatggta ttggatttga
    14041 gattgcacgc tctttagcta cagcaggagc aaaaatcgta tttaataact tgaatcaaga
    14101 atcagttgat gaaggtatag cgcattataa agaagcaggc atcgatgcaa aaggttatgt
    14161 ctgcgatgtg accgatgaag aagcggttca agcgatggtt caacagatca aagaagatgt
    14221 aggttcagtt gatattctag tcaacaatgc agggatcatc aaacggacac cgatgatcga
    14281 aatggatgcg gcagacttcc gccaagtaat cgacgtggat ttaaatgccc cattcatcgt
    14341 ttcaaaagca gttattccgg atatgatcga aaaaggcggc ggtaaaatca tcaatatctg
    14401 ctccatgatg agtgaattag gacgcgaaac ggtcagtgct tatgctgcgg caaaaggcgg
    14461 attaaaaatg ctgacgaaaa acatcgcttc tgaatacggt caatacaata tccaatgtaa
    14521 tggaattgga ccaggttata ttgctacacc gcaaactgct ccattacgcg aaacacaaga
    14581 aaacggtgaa cgtcatccat tcgatcaatt tatcgttgga cgtacgccag cagcacgctg
    14641 gggcgagcca atcgatttgg ctggaccatc tgtcttcttg gcttctagcg catctgattt
    14701 cgttaacggc catgtcttgt atgtggacgg aggcatactt gcctatatcg gaaaacaacc
    14761 ttaaaagagc tttagtacac cgtttagtgg agaggttgtg aaatcagcgg cttgaacgaa
    14821 taccgaagga cagctgatta aacatcattt attcgaaaaa agaggctggg acagaagcgt
    14881 ttaactccaa gaaataagaa ggaattcacg aaaattgctt ttcaaatttt tgtgaatttc
    14941 agcttatttc cgaaggagtt gcttttgctt ccgccgttta tacgtgttta gagcgtggga
    15001 taaaagtgtt ctttactttt gtcccacgct cttttctgtg cgtcttccat acttcactaa
    15061 gtcgatccct cttgcttcgc accaatcctt cactggtccg gaaaagatca gtgattgttg
    15121 gtgtagggcc gcggtattcg ttgcgccgac catagtataa agcagacgaa gctcttcttg
    15181 ccattgcttc atcagtataa tagtttcttc tacgccgtga ttcatcagat gcgtcagtac
    15241 ggttcctgaa gtacccactg catttgcgcc gagacaaagg gctttaaata tgtcataggc
    15301 attgcggata cctccggagg ctaggatttc catagaagtg tctgcttcat ttgcttcaag
    15361 caatgaagag actgtcgatt gcccccagtc agctaaataa ctcagttccc gcttggagcg
    15421 acgggcgttt tcgatctggg tgaagcttgt tccgcttcga ccgctgatat caactgtatg
    15481 aactcctaaa gcagctagat cattgagcgt ttctcttgtc atgccaaaac cgacttcttt
    15541 tacgatgagg gggacgtcaa tggctgtttg agtctcttga ataagtactt tccaattggt
    15601 aaaatcacgg tctccctcag gcataaccaa ctcttgaggt gcattcaaat gaatctgtaa
    15661 ggcatctgcg tgaaacaatc ggatagcttc ttgggctctt tcgacagaag tccctgcacc
    15721 gatgttggca atgattttcc cgtgaggata ttcttgtcgc ataatcgtat aagtatcggc
    15781 taaagaaggg tctttcaagg cagcgctgac tgaaccagtg gcaatcatca aatctgcttc
    15841 tcttgcgacg attgcaagat cttggttgat tttttttgtt ttttcgcttc cgccagtcat
    15901 cgcattgata taaaatgggc tgcttagtgt aaacccaact gcctgtgtac ttagatcgac
    15961 atcagaaact gctatttgcg gcaaagggtt atggatgatc cgtacgaagt caaattcgtt
    16021 tttttgttta tcatgaaagg ctttagctag tgaaacatgt tcgtcttttc gattcatcgg
    16081 ttttcctttc tttgatcgct atagacatgt aacggcagtg gagtgatttc tgctttttcc
    16141 catgcactca ttaaaggaag gatgccagat ttctggtcaa caattacgat tccgcaatcg
    16201 ccaccacctg caccagaaga ttttgcggct ccttcatact gttcagctaa attacacaat
    16261 ttgttcaaag caggcgtttc gatgacgacc ccagtgattg cagataaatc atgcagtaat
    16321 tgtcggtttt ttcgaatcat ctgttggatc aacgttacat tattttcttt aaaccctttg
    16381 atcatttcgt tgacacattc tgtactgttt tttaagaaaa gctgataagc caccattttg
    16441 tcttctctcg aacggtgaac ttgatcgacc aaatcagaag tagaggcagg gctacccgtc
    16501 caaccaatca ataaacgtaa atcttcagga gcaatcaatg gctcaatgga tagacctggc
    16561 caatccaggg caagtaactc actgatagaa tgctgagttt cttgttcttg gagccaagga
    16621 tggtcgaagg ttgagaaagc gatccagcca ccataacagc tagcagcgat gtcgccgcag
    16681 gaaccattat cttgaactgc taaattggct agtgctgcaa tcttgaaaat ctccaactga
    16741 gataaattca acgcgtaaaa aacattcaag gctttgaccg ttgcaacggt cacggcaccg
    16801 ctagaaccca atccatattt ccggccattt gaactgtcta actcacttgt tactttcaga
    16861 tcataaaagg ataaaagaat gtttttttct tgtgcatact tttcagtcaa acgaatagca
    16921 gcaaggatat aatgaaaagg attttcccga atatctaaaa ctaattctcc gttgcgtctt
    16981 gtccaacgta caggcatccc actatattgt gcagattgga tacttccgac ttttcgtgcg
    17041 gattctacag tgactgttac gaattgatcg actgcagcga taactgctgg atgtcctgtt
    17101 tcaacaactg catattctcc ggcaatatag agtttgcctg gtgcagatac ttcaatcata
    17161 aaaatctccc tttattcaat aatcgcaatt cctggaccag catgggcagt aattacttgt
    17221 tctttgctaa ataatttggt aaatgtttct tgtacctcag ataaatggct gttttccact
    17281 aaaactttga cattagggcc agcatccatc gtaaagtagc atggaatgcc tttttttctc
    17341 aattgccgca ccgcatccat cgcttttaag ctgtcaggag accagtaagt gaatggaggc
    17401 tgggcagcta aagtcgttcc atgcattttt agtccgtttc tttccatcgt ttctccgaga
    17461 agttggaaat cttttgtttt gattgcttgt ttcaattgat atagatcccc ttcaacggaa
    17521 tctaaccagc cttgataaaa actggatgtt tcgactgtcc gacgcatccc atttctgctg
    17581 gacacttctt ttttctggtc gttgatcaaa acgaaaacca ttgctaaatc gtcttcgaaa
    17641 gaatcggaag ggactggctt agcgtaagaa cttaagtcgt catggccttt ttcccattcg
    17701 acgaaaccac cgaaaatact tcggcaagct gaaccagacc cgcgtcgagc aaatcttgaa
    17761 agagagagat cgtctaatcc aagcttaaga gcagtattgc aagctcctgc taaagcagct
    17821 agcccgctgg cagaggaggc taatccagct gcggtaggaa cgaaattctg actgattact
    17881 gaagctttta gtgagaggcc ggcttcttta cgaaaaaggt caagaaactg actgactttt
    17941 tttgttgctt tttcgtcttg taaggtgcca tctagataaa attcatctac catatagcta
    18001 tcagaaaaga tgacttctgt ctctgtataa aatgcatcca atgttaacga aaggctgttg
    18061 ttcattggaa ggatgagttc ttcatttttc ttaccccaat atttgattag agcaatgttg
    18121 gtatatgcgc gtgctttgcc tttaaacata ggtatgtact cctaatcctt gtatccaagt
    18181 ttcagcagca cccgcatctt caagtgcttg actgatttct tgggcttttg tttttgtttg
    18241 tgctaatgca atcatacagc caccgcgtcc accgccagtt aatttggcac ccaatgcccc
    18301 ggtatctttt gctgtttgga tcaatagatt aagaaaatca ttgctgattg tcagcttttc
    18361 cagaagtgac tgtgattcat ccattgtctg tgccaacgtt tctggtgaat tctcaatgat
    18421 tgcttgtttt gcttgcttcg tcaagtatcc taatttttgg atcatttgtc cagtttcatg
    18481 aggctgggtt tcaaagaggt gagcgacatc tttgacggct tctcttgttt gtcctttgat
    18541 tcctgtatca gcgacaatca aaaaagcatc gatgttcaaa gaaaagtagt caaaaggatg
    18601 cccttttgta aaataaatcg gctgcaagct gctagtagcg gctgcatcga ttccactagg
    18661 attaccgtgg gcgatttttt ccgaaagctg gacattttct aatagtattt cacgagacaa
    18721 aggaaatgct aagtaatcat aaaaagcacg agtgactgct gtggcgactg cagcgcttga
    18781 tcccattcca cgttcagcag gaatcgtgct ttcaatggtc agttgcaagt ttttttggat
    18841 gtcatgctgt ttttttaagt gaatgaataa ttttttgata ttttttaatg catgaggagc
    18901 ttcggctaaa tttcctgaat agtaggaaga aaccagttga tcgtccattg catccagctc
    18961 ttcaaggaat gcggtgactt ttgttgcata gaaaggaaag gcaatcgctg gttctccata
    19021 aacaaccgcg tgctcgccca tcaatatgat ctttccgctt gattctcctt ggccatagtt
    19081 tgccataatc taactccttc tcacgtaaaa gtgattttta agcaaaaaat aaagcgccca
    19141 aaacgagttg cgctgcttca cgaatttttt tgtccactaa gggtctcaaa aaacattcct
    19201 cctttatttt actaaaacca tctcagaagt aaaactaaga atacgttttc taaagaaaaa
    19261 acactttttt cttaagaatt ttttgacttt tgttcttctt tgactagaaa aaatacaatt
    19321 gacactacta gatattgggg gtatatttaa aaaggttgga gttaaacaca atatttagtg
    19381 atgaagtgag ctggaggtaa atgagcgtga tcgttttagc aggtacaatt ggtgccggaa
    19441 aatcaagttt aacagaaatg atggctgaac actttgatag tcaggcattt tatgaatcga
    19501 ttgatgataa tgaagtcttg cccttatttt atgcaaaccc tgaacagtat gcttttttgc
    19561 tgcaaattta ttttttgaat aaacgttttg caagcatcaa acaagcaatg aaagatgaca
    19621 ataatgtatt ggaccgttcg atttatgaag attcactgct tttccatcta aatgcagatc
    19681 taggaagagc aacagaaaca gaagttcgtg tgtatgatga actgctggaa aacatgatgg
    19741 aagaacttcc ctatgctgcc cataaaaaac atccggattt attagttcat attcgtgtat
    19801 cttttgatac gatgttagag cgtatcgaaa aacgtggtcg ttcgtatgaa caattgtcct
    19861 ttgacccaag tctatacgat tattacaaag aattgaacag acgttatgat caatggtatg
    19921 aagaatacaa agaaagtcca aaaatccaaa tcgatggaga ccgctataat tttgtggaag
    19981 atcctcaagc aaaagaagaa gtcttgaaaa tgatcgaaga aaaattagca gagattcgcc
    20041 aaaccaaagt atccatttga tttacagttg ctcaaataaa gggtaaacct ttatttgagc
    20101 gacttttttc tttatactgg aaacaaatgt caggaggata gagtatgcgg gaaaaacact
    20161 attttaatca agaccgtaat ttttggatca atgtacagca aaacgatgtg ctagctgtta
    20221 aaaacttaaa agaagaatat ggcattagtg acgagatgct gacttattct ctagataaaa
    20281 atgaacgggc gcgcgtagag tacgattctt ttgatgaagc gttactgctt gtatcaaatg
    20341 ttccgcacca gcaaaaagtc gataatcatt atgaaactag tcctatcgct tttattttaa
    20401 aagaagacgg tttgtttaca tttaccacgc cgaataccga gtacgtgatt cgactaattc
    20461 gatcgctttt ggaacggatg ccagacatga gtgtatacag tttattgttt cgtacattgt
    20521 ttttgatttc tgattccttt tttcctttaa tcgaggaagt gaatagtgaa cgtcagcggc
    20581 ttaatctgaa attaagagaa aaaacaacga acaagaatct gctgcaactt tcagatttag
    20641 agatcggttt ggtttactta gttacaggaa caaaacaaaa tgtagtactt ttagaacaaa
    20701 tcaaggcact ggctatttat cgcaagttgt cagaaaagga aaaagaagag ttggacgatg
    20761 cactgatcga agcacgacaa gcagttgaaa tgacgaactt agcttcgcag attttagacc
    20821 agctctctgg cacttataat aatttgctga ataacaactt gaatgacacg atgaaatttt
    20881 tgacggtctg gtcgctgatc ttgaccgttc caactattgt gacaggattc ttcggaatga
    20941 atctccagtt accctttacc cattccgttt tcggatgggg aatcgccttg atcatcagtt
    21001 tggtcttatc tatttggatg cttattgcac tgtggcggcg gattcgttga tctagtcttt
    21061 gatctgcaaa ccaagggctt gggcgatcac aagtgcttgc tcttgattga cgattagtcc
    21121 cgccaaagaa tcttgagata aagcgattgt tgtgaagaaa ttagtcgtaa agtctaatcc
    21181 cttcagagaa gttcgaaacc agttcgaacc ggttaactga ttgtcagaca aaaaaaggtt
    21241 tttccaagtg acctcataaa attcgctgtc ttctaattga caatgatcga aggagacagc
    21301 tttcaagttt gtactactaa aggaagccat cgaagcaata caatcagtga aagtacagtc
    21361 tcgaagataa ctttcggcaa aattcgtacc tgttaattta cattgatgaa acacgacttg
    21421 gtggaaactg ccaccaatcc actccagatt ggaaaaatca catttttcaa aaatgacatt
    21481 actgcattca aaatgacgta aacctgtttt ttgcatcgta agtttttgga tatgactctc
    21541 tcttaatacc atatttttgg catcgagata gctttggtta cctccgttga tcagaagacc
    21601 agagaagatc tcctcatctt ctaaataaaa ctctcccttt gtaagaagag gaagagaagg
    21661 tggaataggt tcgatttttt tcatgtgggt tcacctcgtt tttctttagt atagcaaaga
    21721 agcgggaatc actgctagaa agcctgctaa tctaatcatt ttcagaaaag gaagacaaac
    21781 tgtatgaaaa acattgacaa aaagattagt ttttgctata ctataatggt tgagaaataa
    21841 aagcagaagc acccgcttct cgccttagtt gataatgtca ctgggctaga tagttttcac
    21901 tggttctatt aggtgaagta cgcgcgggtt ctgtaagaga actcgttttt ttattgccgt
    21961 ttattcggta agcttttctt tctctcataa tatttggagg tgaatgacca tagcaaagga
    22021 tatgatggtt aacgacggca ttcgtgcacg agagttacga ttgatcggtt cagatggtga
    22081 gcaattagga gttaagacaa aagcagaagc attgaatatt gcagaacaag caaacttgga
    22141 tcttgtgtta gttgcccctg gtgcgaaacc accagttgcg cgaatcatgg actacggtaa
    22201 ataccgtttc gaacaacaaa agaaagaccg cgaagcgcgt aaaaaacaaa aagtgatcaa
    22261 tgtaaaagaa gttcgcttaa gtcctacgat cgacttgaat gacttcaata caaaacttcg
    22321 taatgcacgt aagttcttgg aaaagggcga taaagtgaaa gcttctatcc gtttcaaagg
    22381 ccgtgccatt acccataaag aaattggtca aaaagtcttg aatcgcttag ctgaagaaac
    22441 tgcggatatt gcaacagtgg aacaaaaagc gaaaatggac ggacgcagca tgttcttgac
    22501 gctggcaccg aaaaacgaca gtaagtaatt tgaatgacta actggctgtt ggacagccga
    22561 tagtcggata acgaaagatt ttgaggagga aatatagtca tgccaaaaca aaaaacacac
    22621 cgcggatcag caaaacgttt caaacgtact ggtaaaggcg gattgaaacg cttccgcgcg
    22681 tttacaagtc accgtttcca cggcaaaaca aaaaaacaac gccgtcaatt gcgtaaagca
    22741 agcatggttt ctagtggcga cttcaaacgt attcgccaac aactagcaaa aatgaaataa
    22801 gaagtacaaa taacagaatc tgaagattta tttttattag gaggaattat acatggcacg
    22861 tgttaaaggt ggaacagtaa ctcgtaaacg tcgtaaaaga gtgctaaaat tagcaaaagg
    22921 ttactacggt tcaaaacaca ctttatttaa aacagcaaaa gaacaagtaa tgaattcata
    22981 caactacgca tatcgcgatc gtcgtcaaaa gaaacgcgac ttccgtaaat tgtggattgc
    23041 tcgtatcaac gcagcagctc gtatgaatgg cttgagctac tcaaaattaa tgcacggttt
    23101 gaaagttgct gaaatcgaca tcaaccgtaa aatgttagct gacttggctg ttaacgatgc
    23161 agcagcattt actgcattgg ctgaccaagc aaaagaagct ttggctaaat aagctaaaca
    23221 attataaaga ggaaagaaga taacgaaaag ttatgttctt tcctcttttt ttatttacaa
    23281 tcctccatca aatgaaacag gacaataacg tataagtcta aaataacata attaaggttt
    23341 tttaattgaa aactgaacat ttatggtcat cctttaatga aagcgctaac tacataagtt
    23401 agaatgagtt tgactgatat attagtttgc ttatcagaaa aacaaaatgg acggaggaat
    23461 aacatgagtg atattattat tggtattttg ttagttatct cattcttttt catggtctgg
    23521 tactgtgtaa aaggctacaa cttgatggtc ggcttttgtc atcatggcta ctgtatggat
    23581 gggattagcg ctagtcggaa acacgttttc accaaactcg gcgatggaag ggcaaggagt
    23641 aattgatgtg ttgacgcata tctatacgac ggggccagct gagtacgcca aatcgatctt
    23701 ggtaaatgtc ttctttggtg cctttttcgg gcgtgttcta gtagatagcg gaatcgcagc
    23761 taccctgatt cgaaaggtag ttgaacttgg aggggacaag ccaagaatca cgatgagctt
    23821 actatgtatt gtaaccgctg ttatattcat gtcgatgaca ggaatcggac cagtgatttc
    23881 tattgcagtt attgtgttgc cgatcttgat gtcattaggt atttcagtac cggttgcact
    23941 gttctcattt atgggctcga ttatggctgg aatctttgct aatatcgtca atttcaaaca
    24001 atatcaaacg atttatgctg gtttcaatcc agcggctgaa agctatactt ataatgacta
    24061 tttccaaatt ggtatgattg ggatgattgt aagcttagtt gtggtactaa cggtcgctaa
    24121 tatatcaatg aacaaaaaga aacgctatgc aatggcagca aacgtaccag ctgaaggtgg
    24181 cgacgcacca atgatctcat ggttagccgt attattacca gttttagggg tcgttctttt
    24241 agatcttcca atcatcttag gttttattct agcaggtatc tgggcattgc tgtttactgg
    24301 aaaattgcgc ggaggttata aagaaatctg tcgtcagttt gcgaaattat ttacagatgg
    24361 cgcagtcgat gttgcaccaa tggttggttt cttgatgacc ttagcgatgt tcaataattc
    24421 agctgcgtat gcttctcctt atttttcagc tatttttgga gattggatac cgcaatcacc
    24481 attagtcttg gcaatcgtat ttgctatatt gacaccacta ggatttttcc acggtccaat
    24541 gaatctagtc ggttcaggct cagctatttt agctgtcgtg ttagctgtta atccaacgat
    24601 gtcgccagcc tttttgttcc cgttatttgc aattacaacg attgctcctc agcatttaga
    24661 tattacacaa tcttgggttg cttggggatt aggttataca aaagtaactt ctcgagaata
    24721 tatgaagaaa tccatcccaa ctggttggat catcggtgcg atcttatgcc tgatcacgtt
    24781 cttattgtac gggaacgcct agtaaacaaa acgtcgataa aaacgattag ataaatggag
    24841 gaaaagaaaa tgaaacgttt tgtgcgaatg ggaattgatg ttggcggtac acatacgaaa
    24901 gcagtcgcaa tcgataatgc gacgcacgaa atcatcggaa aatcttcagt gaaaacaacc
    24961 cacgatgatg tccgtggagt agccgcaggt gtcgttcagt cttttcaaaa ttgtttgcgg
    25021 gaaaataata tcagtccaga agatgttgtg tttgttgcac attctaccac acaagcgact
    25081 aatgcactga ttgaaggaga cgtagcaaaa gtcggcgtaa tcgggatggc aaaaggtgga
    25141 ttagaaggtt ttttagcaaa acgacaaact agattgaacg atatcgattt agggaataag
    25201 aaaaaaatag agattgtcaa tgcattctta cctgtcaaaa acttaaatgt agaccgtgtt
    25261 tcagaaacga tttcttcttt agaaagagaa agagcagagg tcttggtttc ttctatggca
    25321 tttggagtag ataatgggga accagaacga gttgtttatg aagcagcatc tgtgaaagcg
    25381 atccctacaa cgatggcttc tgacatcaca aaattatatg gattgacaag aagaactcgg
    25441 acagcagcaa tcaacgcatc tattttacca aaaatgctag atacggcaac ctcgacagaa
    25501 gactccgtac gagaagcagg ggtcaatgtt tctttgatga ttatgcgcgg agacggcgga
    25561 gtaatggaaa tcaatgaaat gaaaaaacga cctgttttaa ccatgctttc cggtcctgct
    25621 gcatcagtca tgggctcttt gatgtacttg agagcgtcta atggtgttta ttttgaagta
    25681 ggcggaacaa caaccaatat cggggtcatt aaaaatggac gtccagcaat cgattattca
    25741 attgtaggtg gccacccaac atatatctct tcattagatg ttcgggttct aggtgtggca
    25801 ggcggttcga tggtacgggc taatcagtca ggaatcatcg atgtcggtcc acgttctgca
    25861 catattgccg gtctagatta tgcagtattt acggagacag aaaaaatcaa aggtcctaag
    25921 gttgaatttt tctctcctaa agaaggagac cctgctgatt acgtcaaggt agtgatggaa
    25981 gacggagaag aagtgactat tacaaataca tgtgcagcca atgttcttgg attagtgcaa
    26041 gaagaacatt tttcttatgg aaatgtccca tctgcacgaa aagccataca ggcattagca
    26101 gactattgtc atacgacggt tgaagacatc gcagagcaga tcatggaaaa atcctatgct
    26161 aagattgaac cggtgatttt agaattggca gacaagtatc acttggagaa agatcagatt
    26221 tctcttgtag gtgtaggggg cggcgctgcc tcattgatta cttactttag taataaaatg
    26281 ggtgtgaaat attcgattcc tgaaaacgcc gaagtgattt cttctattgg tgtagcactt
    26341 gcaatggtcc gagatgttgt agaacgtatc atcccttcgc caagtaaaga agatatacga
    26401 tcgttgaaaa atgaagcgat gaataaagcg attgaatcag gcgctacacc agaatcgatc
    26461 gaagtacacg tagaaatcga tccacagact tcaaaagtaa cggctatcgc tactggttct
    26521 acggaagtga aagcaaccga tctaaccaag gagatcacaa cagaagaagc actagagctt
    26581 gctgcagagg atatgcgatt aaataaaaat gaagtttgct tattggaaaa tacacctttc
    26641 ttttatgtat gcggcgaaca gaatcgttca aaaaatgctg gaagtcttcg gatcatcgat
    26701 caaaaaggat ttatcaaggt ccaacgtgga catgcttcat gtatgaaaac aactgcagcc
    26761 aattatatga cagctgtcga acaactttgg gaagatatgg cagtttatca aactgaattg
    26821 attgcccgac cagaatttta tctctgctta ggagcaagag tctctgattt tacagcgacg
    26881 gatctggaac aactacagct tttgatggat ctggaagtat ctactatgga accggaggaa
    26941 gaagtgatcg tcgttgccgg aaatatcaaa caaacataag ttagaagagt tactaaacga
    27001 attacaacag atccccgaag aaatttgggg attctaccag tttcggcgag atttattttg
    27061 gaaaaagatt cctttgtcca agcagaaaat tcttatacaa caatcaatcg attgtggtat
    27121 cgaaacggca tgtagcatca aaaaaaagta cccttttgcc gatgtaggag aaatctgtga
    27181 acaaatggct gttccaatcg tttcctgtga atcagagcaa ataaacgaac gaatcacttt
    27241 tgtaacttat gcagaagatg aagggatccg attgatgacg gagccgttag aaaaattaaa
    27301 atgttcagga ctagcttcga tctctaaaga aacagcgcaa gctttgatta ttggacatga
    27361 actatttcac cacatcgaag cttctgttaa gggaatttat acgcaaagcg aaaaaattgt
    27421 gctgtggcgg ttgccttttt atacacatca atcaaacatt cgtgcattga gcgagatagc
    27481 agcgatgagt ttttcaaaag agatgaatca aagcgatttt tcgccttatg tattagaggc
    27541 agtcttactt tggccatata atgaaattcg tagccaaggg atcttagaag aaataaaaga
    27601 aatagaaaaa agatgtacgg agtatgattt agattacaaa taaagagact gtgacaaaag
    27661 tcgtgtcaca gtcctttttt tccgaataaa cggtgttctc ctgtcaactc ctcggtcaca
    27721 agtcaaaata ttcaacaaac atgagaagct gttttttgaa attgttcctt gtgactcgga
    27781 gatgaacggc aggctcacga cctcttggaa aaaggtgttc aaaagcttgc atctgcggta
    27841 ataagaacag ccaagttaaa aacataagaa gctattttta cttggctgtt cttattactt
    27901 gatgcagggc agctttttca caacctctca ataaacggtg tgcaatcagc tgaccctcgg
    27961 tattagctca aaaaaaacgg caaaatatga ggaactattt tgccgtttat ttctctaata
    28021 ctcaggtcaa aacgctgatt ccaaaacctc ttatttgatg ggtctgtcct gaatatcttc
    28081 aaaataatcg gggtaattat gaatcacgta ttcttgctcc tcgataatca tatgtaaatg
    28141 catcgtgcaa agaaatccga cttcatcaaa gttatgatca atcattgatt gaagcattgt
    28201 ctgatgttca tctaatactc gaccccagtc taattctgaa atcgtcaaac gtaaccatct
    28261 gaaacggttc aagtgcgtac tataactttc tagccaatcc caaatttctc gttttccagc
    28321 aatttcataa caagttcggt gaaaatcagt atccaaatca aaaaaagctt ttttattttg
    28381 agcgatcact gcctgatcgg tttgttctag tattttttct agtttagctt gatcttgctt
    28441 ggtcattttc gcactgcatt cttgcaggat cggtttttcc aacttttccc gcgtataacg
    28501 ggctaatgaa gcagatcgga tatcgatttt tgaaacataa gtgccggact gtggaatggt
    28561 ttttacaagc tcttgattgc gcagttggat cagtgcttct cgaatcggtg tacgcccgat
    28621 agctagttgt tcttctaaca ctttatcgga gattttactt ccaggtgcta actcggaata
    28681 aatgatttgt ttacgtatag attcataagc ttgctgttgc aaattattga taaccattta
    28741 accttccttt ctatgcagat ataatctatt ataacataaa tcgcttacaa atcgtgtggg
    28801 cataaaaaca cagtctgcaa aaaaagccct gataggcatg cctattagag cctcgaaaag
    28861 aaaaatattg aatcaatctg gtcgtacctc ttggatcggt ttgacacgac cgccgattct
    28921 tttagcaaaa gcttcggatt cttttttgtc tttgaaaatg agcagattgt ctaagttttt
    28981 ttcccgagtg cgacagccag ttttatcgat gtaaccgtgc attactttaa cgacatacat
    29041 ggggatcacc tgcttttttc ttttcattat acgaactact cgacacaata caagaaaaat
    29101 gcttagacgg tctttattaa gaatttacat catccaaaga agcgtacgct tggaataact
    29161 gttctaactc cggattgact ttgctcagct tcactaatcg ttgcgaatca gaaagtaaaa
    29221 aacagtgttt actactgcca gtagttttta attttttgtc ttcagttccc acaatttcgt
    29281 agctaaaatc caaccgtgtt cccgtatact tttggatagt tgggtggatg ctcacttttt
    29341 ctccgtaatg gatcatttct ttatagctgc aatttacttc cagaacgggg atgatgatcc
    29401 cagattcttc gattttttta tagggaaacc ctagatgtac caataaggct acccgagcct
    29461 cttcaaacca gcggatataa ttgctgtgat gaataatccc catttgatct gtttcataat
    29521 aaaaaggttc tcgcacataa cctgtaaatt tctccatgac tcatgcctcg ttttccattg
    29581 attttttaca aaaataattc atccacttcg gaagtgcttc gctgatttgt gtcggcaaaa
    29641 caatatattg ttcttttgcc agctggtctc caatgaaact atgaagataa acagctgctg
    29701 caatcgtttg gtcattttga ggaaactggg ctaaaaatcc tgtgatcatc ccagctaaag
    29761 tgtcacccat gccgcctgtt gccatagcag cagaacctaa aggattggtg tagaagtgct
    29821 ggtgaccata aatttttgtc tcatgacttt tcaaaacgag cacagcaccg atttcctgtt
    29881 gcttcttttt gttttcttta tcccgttgtt cttgtatcgg tagagaactc aaccgttgcc
    29941 actccatttg atgaggtgta agtacagttt gttcgggata gggtagcaaa agatgattag
    30001 caacaagtaa acttaaagca gagccatcaa tcaccagcca ttgttctttt gattgttttt
    30061 ctagtacttg tttgaaacgt tccaaactct tagaagaagt acccaatccg ggaccaatca
    30121 agagtacatc tgcttgcttt attacggaaa gaaactggtc agtatccgtc caatctacaa
    30181 tcattgcttc tggcagatga ctgtgaagag cactccgatt tttttcatca gttactacag
    30241 tcgtgagacc cgctccactg tgtacggcag ctaaagcact cataatgatt gctccgccat
    30301 attgttcatt gccgccaatc aaaacgatac gtccaaatgt tcctttatgt gaaatttttg
    30361 gacgaggtgt gatgactctt ttcactagtt gttcgtttat cagcatcgaa aaacctcctt
    30421 ttactcatta tagcgtatat tttttttaga gaaaaacaga taagaaaagg aattgtcaga
    30481 ttttttgaac tatttttttc tgtaatttgt ggtatcatga acacgaagac ttaaataagt
    30541 ggaggattaa ctttatgaca attgattggc aaaaagaagt agaagcacgt aaagaagact
    30601 tgcttgaaga cttgaaaaat cttttacgtg tcaacagcga gcgcgatgat tcaaaagtaa
    30661 caccagatgc accatttgga cctggacctc gcgacgcatt gaaacatatg ttagcttatg
    30721 gcgaacgtga tggatttaaa gtaaaaaatg tcgataacta tgcagggcat attgatcttg
    30781 gcgaaggaga cgaaacgcta gggatatttg gacacatgga tgtcgttcct gcaggagatg
    30841 gctgggacac agatccttat gaaccagtaa tcaaggatgg aaagatcttt gcacgcggat
    30901 caagcgatga caaaggtcca agtatggcag cttactatgc gatgaagatc attaaagaac
    30961 ttgacttgaa actatcaaaa aaagttcgct tcgtagtcgg cagcgatgaa gaaagcggct
    31021 ggggcgatat ggcttattat ttcgaacatg aagaagaacc agattttggt ttctcaccag
    31081 atgctgaatt cccaatcatc aatggtgaaa aaggaaatgt ttctttagct ttacgcttca
    31141 aaggcgacaa tgcaggagat tatgtcttga aatcttttgt ttccggactt cgcgaaaata
    31201 tggtgccagg aacagcaact gcagcacttc aagtaccaag tgcagatgca gctatcgcga
    31261 tggaagaagc attttaccaa ttcatcgaag caaatccagt aagcggaacg attgaagcgg
    31321 acaatacgta tgtaaaaatc gaattagttg gtaaaggcgc acatggtgca agcccacaat
    31381 ctggtatcaa tgcaggttct ttcttggcat tgttcttaga taactatgaa ttcctaggat
    31441 ctgctaaaca atttatccat gtagcagctg cttatgtcca tgaagatttt tatggagaaa
    31501 aactaggagt cgcttatgaa gatgaaaaaa tgggcaaatt gacaatgaat gctggactat
    31561 ttgcttttga agaaaatggt acagaggaag cgaacttcat caatatgaat ttccgttttc
    31621 caaaaggtgt aacagtagac ggattgcaaa gtgacatcga acaaacagtg gggcaagaag
    31681 gcgcaacagt tactcgaggt gctcgcgtaa tggaaccaca ttacgtacca atggatgatc
    31741 cattggtggc tacattattg caagtctatg aagaccacac tggagaaaaa ggctacgaac
    31801 aaatcatcgg tggcggaact tacggacgtt tgttgaaacg tggcgttgct tatggagcga
    31861 tgttcccagg atatactgat acaatgcatc aagcaaacga atttatgagt cttgatgact
    31921 tgttcagagc aacagcaatc tatgcagatg ccatctatcg tttggcgaaa taaaaatact
    31981 aaaaaactcc ggctatctct gacagctgga gtttttatgt ttaaaaatga attagtacat
    32041 cggtattctt aaaagtaatt tagttcgtct tttgatttct caggagggat caaaaaggcg
    32101 aaagcactaa gtatagcaaa acttgtgatg atttgcgtac gatacgtaaa ccaataatga
    32161 atctgtgagt gattagctaa aatattgtac caaatatacg gcgttacagc agctaaaaca
    32221 aacaaaacta gaagttggaa cggacgccag ctatttcgtt tacgataagc aaaatagaga
    32281 aaaacaagac aagtaatcaa tagcaataat attgctactt ttggatacat caatctaata
    32341 ttgcttttca gcatctctaa tcgatgcaac gggtatttgt cgtttccttc cgttcgaaag
    32401 aggatttggt tgattgcatc ttttacaatg tttttgttca atatgatcga tgataggatc
    32461 catttagcca cccatgtcaa accatagcct aatccccaaa agataccggt aaaaacaaaa
    32521 ctgcctactt gtttgctcaa tgaactgcgt ttatccatta aatacttatt tgttacataa
    32581 taaataatga tcacaggtat cccccaagag atgaccggtg ttgtcaacaa gtcaaaaaag
    32641 ttagctaaag aaccaatgac aaagaaatac aagaataatt gttggttggt gttggagagt
    32701 tgtggttttg ttaaaaatag taatacagac aataaagtca aaatccaaat atttgaaaat
    32761 tgcatactga agaaaaaggt tgcagcattt cctatagcaa gcgaaatcaa caaggctgtc
    32821 gttaaataga tgtcgctaca cttggttaaa aagtagctcg tcaatccaat cagcagaaaa
    32881 agaatggctg cgtaaatcat tcgaatactc cccaaactca tccctagcag taaaggtcgt
    32941 aaaaaaagct gataaccatg ccaataacgg ggataatccg ccatagacat cgcgttctcc
    33001 aaggagttca attcaggatc tgcttttgtt tttcgtatca tcaaatgatc agtaaaatta
    33061 tctaaccgag tgcctagcat atttccttga ttgatagaag gatacattcc ttctttttca
    33121 accatctgta gagatttttc tgcattcgat tcaatagagt gctgcggaat gaaaaaagta
    33181 attgtcagta gtacgcaata gctagcgatc aagagaacaa aagcaaaaaa tatcttttta
    33241 aatagtttca tgagtatctc ctatatctgt tttcattttt tctcttttat ctagtaaaaa
    33301 aggagagagg atataaacag gtagacctaa tgctaaaaca aacacatacc gtaaactaaa
    33361 agccacagga gtagcaatca taatagtagc aaaaacaagc ataccgggaa gaaaaaggag
    33421 taaataccga tattttttct taaagattgc taaaagtgca ctgccgatag ctaaccacag
    33481 taatgtgccg gaaccaaaga aatcacgagc atcgatgatt tttttcattc ctgacaatcc
    33541 agtaagatgt tcaatcaaat ctacttgctt catcccataa ttattcgtgt ttacacgagt
    33601 atctaaaaag ccgtaactat tttttgtacc aggtacccag aacccgaacg tttctaaaat
    33661 ataagcatct gtatagattt ttatattact tggtaaattt cttgcccata gtgatagaaa
    33721 ttctttttta tgtgtattca aaaactcatt gttaaaattt ttatgccact tgattgagtc
    33781 tgcaagacta ggagaatacg cttcataatc ttttatcggg agtagctgat tcatgaatgc
    33841 tttatctttc ttagacaagt caccatccat aacaatagtt ctagaaattt gctgcaaagg
    33901 aacacctaat tgttcagcag tttctgtagg aatagcataa cgagccatca acgggtggat
    33961 gagtagtcca gcaatcactg tataacccag caccacttga aagaatgctt gatacctttt
    34021 ttgtttcaaa ataatcaagc cgcaaatcaa acctgtgccg gttactacat aaatcccatt
    34081 atttcttaga taggtggtca gtaaagcaag gatgattacg taaatctgat tgagaatcgt
    34141 tggcttttct cgttgaaaaa taaagtcaaa aaccgctaag gaaaataaaa agcaagcgat
    34201 agagaacaaa gtatcttttt gcatgttcaa ggcgtagatt ggaaaaaccg gtgcaaatgc
    34261 aaaatagcat aaactaatga gtaccggaaa aaaatggatg tttcttttta acagccagca
    34321 aagagaataa cttatcgctg aagctgttat caatgtttga aaaatcgaaa ataaaaatac
    34381 accgtgattg atcgtatcag ttggtaaatc atgaacaaat agcttgacca gtaaggtgaa
    34441 taagactgga tgatgattgc ttataggaac atcacccata gattgggcga ccgaacttaa
    34501 cgaatcaggc aaaaccactc caggatacaa tgccaacaga taaggaagcc aacacagaac
    34561 taaaaagaca gtaaagaata aaaaatattt tcttgaagag aaagcctgtg tgttttgagc
    34621 agtaaaaaat gtttccagtt ttggcattga taaataaatc gattgtaaaa ataaaaaaga
    34681 atacacaaaa atggagaaat aaacaagaaa gtaaaaaata cttccatcag caatcgttgt
    34741 ctttgtatag tttgctgaga catactgaaa ttgtatctgt ctgccaatag aaagtgtaag
    34801 agcaaaagct gcagaaaaaa acaatcgggc aatagctgca actttgtttt ttattgaaaa
    34861 caactctatt ccatattgct taaaagcaag tagcaaaacc atggctaaac caatgattac
    34921 tgttggattc aaagttccta gaccataaga agtaaaaaaa gcaaaacctg acgcaaataa
    34981 agttgcaaaa aaagtaggaa atagattctc ctttttctta tgcataaaaa aggcctccta
    35041 cttcatttct ttattttgat attcgtggta gacatggtat aaattcaatt catattcttt
    35101 cttggcattt gtgacgaccg tatccaaaat caaccctgtt actaatgaaa gaagggctaa
    35161 agtcatcaat ccagtactta aaatcgcaga agggatgcgt gtaataaagc ttgtcagcat
    35221 atattcacga atcacaggaa tccccatgac caatccaaat aaaaagaaga agatggtcca
    35281 gatcccaaag aaaagcaaag gtttataatc tttgcacatt ttgatgatca tcatgatcac
    35341 tttgaatccg tcagagaatg tattcaattt tgattcgcta ccttcaggac gatcgcgata
    35401 atcgattgga atctcaacga acttgaattt tttatctaaa gcatgaatcg tcaattctgt
    35461 ttcgatttga aaaccagaac tcataatagg gaagcttttg acaaaaattc gattgaatcc
    35521 tcgatagccg gtcatgacat cgcgaatttt tgttttatat aagtaagtaa ttgtattttt
    35581 aaccagatta ttcccaaaat catggaaagg acgcttattt tcatcaaaat aggttccatt
    35641 tgatagacga tcgccaatga ccatatctgc ttcgccagaa cgtaatttct caagcaaacc
    35701 atgtgcagct tctgcaggat aagtatcatc accatctacc atcaaataat aatcggcatc
    35761 aatatcaaaa aacatttgac ggattacatt tccttttcct tgacgtggtt ctttctttac
    35821 gattgctcca ccttgaacgg ccaattcgta tgttcgatcg gtcgaattat tatcatagac
    35881 ataaatatct gcttctggta attccctttt aaaatcttca ataacagttg aaattgtcgc
    35941 ctcttcgtta taacaaggaa tgagcacggc aattttttca cgtgattcca tttgtcactc
    36001 tccttatata ataaaactaa tctt
//
LOCUS       DAIGOK010000012        34636 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.012, whole genome shotgun sequence.
ACCESSION   DAIGOK010000012 DAIGOK010000000
VERSION     DAIGOK010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.012"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            591..1280
                     /locus_tag="NOA01_000446"
     CDS             591..1280
                     /locus_tag="NOA01_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCI0631672.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="NOA01_000447"
     CDS             1267..2475
                     /locus_tag="NOA01_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCI0631673.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="NOA01_000448"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="NOA01_000448"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCI0631674.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="NOA01_000449"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="NOA01_000449"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCI0631675.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="NOA01_000450"
     CDS             complement(6578..7273)
                     /locus_tag="NOA01_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HCI0631676.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="NOA01_000451"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="NOA01_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCI0631677.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="NOA01_000452"
     CDS             complement(8472..8663)
                     /locus_tag="NOA01_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HCI0631678.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="NOA01_000453"
     CDS             complement(8684..9574)
                     /locus_tag="NOA01_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCI0631679.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="NOA01_000454"
     CDS             complement(9561..10328)
                     /locus_tag="NOA01_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCI0631680.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="NOA01_000455"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="NOA01_000455"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCI0631681.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="NOA01_000456"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="NOA01_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCI0631682.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="NOA01_000457"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="NOA01_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCI0631683.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="NOA01_000458"
     CDS             14899..15282
                     /locus_tag="NOA01_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631684.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="NOA01_000459"
     CDS             complement(15450..16208)
                     /locus_tag="NOA01_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HCI0631685.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="NOA01_000460"
     CDS             complement(16226..17044)
                     /locus_tag="NOA01_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCI0631686.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="NOA01_000461"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="NOA01_000461"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCI0631687.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="NOA01_000462"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="NOA01_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCI0631688.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="NOA01_000463"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="NOA01_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCI0631689.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="NOA01_000464"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="NOA01_000464"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCI0631690.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="NOA01_000465"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="NOA01_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HCI0631691.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="NOA01_000466"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="NOA01_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCI0631692.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="NOA01_000467"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="NOA01_000467"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCI0631693.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="NOA01_000468"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="NOA01_000468"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HCI0631694.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="NOA01_000469"
     CDS             27086..27385
                     /locus_tag="NOA01_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCI0631695.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="NOA01_000470"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="NOA01_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCI0631696.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /locus_tag="NOA01_000471"
     CDS             27973..28209
                     /locus_tag="NOA01_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCI0631697.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="NOA01_000472"
     CDS             28352..30325
                     /locus_tag="NOA01_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HCI0631698.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="NOA01_000473"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="NOA01_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCI0631699.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="NOA01_000474"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="NOA01_000474"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCI0631700.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="NOA01_000475"
     CDS             33204..34496
                     /locus_tag="NOA01_000475"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCI0631701.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10554 a         6733 c         6844 g        10505 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagac caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggcggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatct
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct tgaaaactat
    25441 atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga aaaagcagaa
    25501 gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg aatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta agctagcaag
    26881 aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataatcag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattggatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaac gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atatcgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaacact gtttattcag aataagggac tgtgacaaaa accgtgtcac
    34621 agtccctttt tccgaa
//
LOCUS       DAIGOK010000013        33510 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.013, whole genome shotgun sequence.
ACCESSION   DAIGOK010000013 DAIGOK010000000
VERSION     DAIGOK010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.013"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..576
                     /locus_tag="NOA01_000476"
     CDS             <1..576
                     /locus_tag="NOA01_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631702.1"
                     /translation="LTILFFLCYSYLKTSHAPSSALGWLLLDHSDITEAEENPFFLIL
                     SSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSFLAILFYSFNHNTLFFFIMN
                     GLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSLFSFFVLALMPLTNPSTIQV
                     AGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(710..835)
                     /locus_tag="NOA01_000477"
     CDS             complement(710..835)
                     /locus_tag="NOA01_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCI0631703.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(969..1862)
                     /locus_tag="NOA01_000478"
     CDS             complement(969..1862)
                     /locus_tag="NOA01_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HCI0631704.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(2044..2970)
                     /locus_tag="NOA01_000479"
     CDS             complement(2044..2970)
                     /locus_tag="NOA01_000479"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HCI0631705.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(3055..3816)
                     /gene="pflA"
                     /locus_tag="NOA01_000480"
     CDS             complement(3055..3816)
                     /gene="pflA"
                     /locus_tag="NOA01_000480"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HCI0631706.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(4060..6291)
                     /gene="pflB"
                     /locus_tag="NOA01_000481"
     CDS             complement(4060..6291)
                     /gene="pflB"
                     /locus_tag="NOA01_000481"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCI0631707.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(6568..9018)
                     /gene="parC"
                     /locus_tag="NOA01_000482"
     CDS             complement(6568..9018)
                     /gene="parC"
                     /locus_tag="NOA01_000482"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCI0631708.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(9030..11075)
                     /gene="parE"
                     /locus_tag="NOA01_000483"
     CDS             complement(9030..11075)
                     /gene="parE"
                     /locus_tag="NOA01_000483"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCI0631709.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(11241..11675)
                     /locus_tag="NOA01_000484"
     CDS             complement(11241..11675)
                     /locus_tag="NOA01_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCI0631710.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            11802..12440
                     /gene="plsY"
                     /locus_tag="NOA01_000485"
     CDS             11802..12440
                     /gene="plsY"
                     /locus_tag="NOA01_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCI0631711.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGLNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(12572..13447)
                     /locus_tag="NOA01_000486"
     CDS             complement(12572..13447)
                     /locus_tag="NOA01_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HCI0631712.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(13529..14320)
                     /gene="codY"
                     /locus_tag="NOA01_000487"
     CDS             complement(13529..14320)
                     /gene="codY"
                     /locus_tag="NOA01_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HCI0631713.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(14338..15738)
                     /gene="hslU"
                     /locus_tag="NOA01_000488"
     CDS             complement(14338..15738)
                     /gene="hslU"
                     /locus_tag="NOA01_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HCI0631714.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(15752..16300)
                     /gene="hslV"
                     /locus_tag="NOA01_000489"
     CDS             complement(15752..16300)
                     /gene="hslV"
                     /locus_tag="NOA01_000489"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HCI0631715.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(16337..17242)
                     /gene="xerC"
                     /locus_tag="NOA01_000490"
     CDS             complement(16337..17242)
                     /gene="xerC"
                     /locus_tag="NOA01_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCI0631716.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(17440..18816)
                     /gene="trmFO"
                     /locus_tag="NOA01_000491"
     CDS             complement(17440..18816)
                     /gene="trmFO"
                     /locus_tag="NOA01_000491"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HCI0631717.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(18785..20863)
                     /gene="topA"
                     /locus_tag="NOA01_000492"
     CDS             complement(18785..20863)
                     /gene="topA"
                     /locus_tag="NOA01_000492"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCI0631718.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(20985..21842)
                     /gene="dprA"
                     /locus_tag="NOA01_000493"
     CDS             complement(20985..21842)
                     /gene="dprA"
                     /locus_tag="NOA01_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCI0631719.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(21898..22665)
                     /locus_tag="NOA01_000494"
     CDS             complement(21898..22665)
                     /locus_tag="NOA01_000494"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCI0631720.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(22658..23518)
                     /gene="ylqF"
                     /locus_tag="NOA01_000495"
     CDS             complement(22658..23518)
                     /gene="ylqF"
                     /locus_tag="NOA01_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HCI0631721.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            23778..24176
                     /locus_tag="NOA01_000496"
     CDS             23778..24176
                     /locus_tag="NOA01_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCI0631722.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            24455..24955
                     /locus_tag="NOA01_000497"
     CDS             24455..24955
                     /locus_tag="NOA01_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HCI0631723.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(25038..25589)
                     /gene="lepB"
                     /locus_tag="NOA01_000498"
     CDS             complement(25038..25589)
                     /gene="lepB"
                     /locus_tag="NOA01_000498"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCI0631724.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(25741..25968)
                     /locus_tag="NOA01_000499"
     CDS             complement(25741..25968)
                     /locus_tag="NOA01_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HCI0631725.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(25965..26483)
                     /gene="msrA"
                     /locus_tag="NOA01_000500"
     CDS             complement(25965..26483)
                     /gene="msrA"
                     /locus_tag="NOA01_000500"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCI0631726.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(26503..27216)
                     /locus_tag="NOA01_000501"
     CDS             complement(26503..27216)
                     /locus_tag="NOA01_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HCI0631727.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(27219..28115)
                     /locus_tag="NOA01_000502"
     CDS             complement(27219..28115)
                     /locus_tag="NOA01_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCI0631728.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(28273..29115)
                     /locus_tag="NOA01_000503"
     CDS             complement(28273..29115)
                     /locus_tag="NOA01_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCI0631729.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            29247..29900
                     /locus_tag="NOA01_000504"
     CDS             29247..29900
                     /locus_tag="NOA01_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCI0631730.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(29963..30490)
                     /locus_tag="NOA01_000505"
     CDS             complement(29963..30490)
                     /locus_tag="NOA01_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCI0631731.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(30510..31457)
                     /locus_tag="NOA01_000506"
     CDS             complement(30510..31457)
                     /locus_tag="NOA01_000506"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCI0631732.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(31469..33355)
                     /locus_tag="NOA01_000507"
     CDS             complement(31469..33355)
                     /locus_tag="NOA01_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCI0631733.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
BASE COUNT         8819 a         7040 c         5785 g        11866 t
ORIGIN      
        1 ttgacgatct tattttttct atgttattca taccttaaga cctcacatgc gccttctagt
       61 gcattaggct ggttgctttt ggatcattct gatatcacag aagccgaaga aaatccgttt
      121 tttttgattt tgtcttctct ttgcggctta ctgttttctg tttatttcgg tgcctatttt
      181 tttcatcgac atcctggtac gttaaacgaa gcttcaagcg tattgattgc atttaaaacg
      241 ttttcttttt tagcaattct tttttattcg tttaatcaca atacgctttt cttctttatc
      301 atgaatggtt tagtcgtgat tttatccatt tttacttatt ttattttttc actcattttt
      361 agccaattga aatgtccgct cttctttcga ttagttcctg tcacctgttt tatttacagc
      421 ttgttttcct tttttgtcct tgctttaatg ccactgacca acccttcgac gatccaagtc
      481 gcaggaaatc tgttggatat gctcttcatc gtcagtctag gtgtatttat gtggcaaaga
      541 aaaaacaaac aaaataaaaa gcattttgaa gcctgactat tacgacttca taaatgcttt
      601 tttagtttta aacacttcaa tctaaaagaa taacccaaga ttggataaat tattaatgac
      661 tcgccattcg tttcttagtg attctggatt atcaaatgga cttcctaatc taatcagcta
      721 gttcttccca taaaatgcca tacttgactg aatcataata ttgattattt aaaaatctaa
      781 cttttcttat cactacttcc tttgtcattc cacatttctc accatttttt tgcattcctt
      841 tgtttccaga ccatgtagtg aatctaatag gtggtagata ggcaaaatac tcaaataatt
      901 cttttaacca taaaggaaga gaaaggctcc tcctgcaaga gtcagtataa tttttgctta
      961 ctgctctctt aggttttact cactaaaact gttccatatt ctttgatata agaaaatgca
     1021 tgcggtataa atttcttgct cagctcagaa aagatttgtc gtttaagaaa ggccatttcc
     1081 tccattgtca atgaaggatc caatgttggt aattcaatct caaaattgtt tttttcgaca
     1141 ataggtaatt ctaatgtgaa tgattcttct tcctttatag tattcgtcac aagtagagac
     1201 tgatggaaaa tactaggccg gcgattttta tatttattct tacgtatcct gtcaacgata
     1261 acttctattt gatcaaaaaa gtacgattca tttacgtctc caataagttc ggctaattct
     1321 ttgaatgcct tcgttacagg aagctgattc ttctgtacaa aaaagtaatt agccaaagta
     1381 agcgggaagt gttctataga ggaaagtgtg ataaaacggt acttctcatt tcctgcagtt
     1441 gtcctcagca attctgtctc tcggctgttt cttactccat aaaaatacgc ttctttccgt
     1501 tcatcttcga aacaatacgc ccaaataact tctcccatcg ctaatttttg ttcatcaaca
     1561 gagagtctct tcaattgttc agctatggtt tcagcagccg aagtggcttg ttgcaagtaa
     1621 tctttaggat caaatattgg aaagttcaaa tgatatcttc gttcttccct cttgattaat
     1681 cctgcatcga tcaataaatc aagaaaatgg gaaaatttat tttgcgaaaa ttcttttttc
     1741 aattctctta gaatcgtttc ttgttctcga tgttcatata gataatcggt caatgaagaa
     1801 aaaagcggat catcggtcag tttttgataa aagttttcct tattttttga ataatagttc
     1861 atatatgttc tcttttctat ccgttttatt ttacttaaaa ggcttttacg taaaaaggag
     1921 gaagaacaag agtcataatt gactgttatt ctcctccatt tcttcggcct ttagtatgtt
     1981 ctatgtttct ttttatatca atcgcttatc cattcatttt gaatgcttca tactaccatt
     2041 tgattagcta aatacagccg tcaattgagg tacgacttgt tttttacgtg aaacgacacc
     2101 tttaagcaaa gcacggttgt tggtcaaagt tgtttggaaa gcttcttcta ctttttcttt
     2161 tcctttacca gcaacaagta attctgaatc actgtctaag atgtttgtga caactaatac
     2221 gaacagatcg tagccatttg ctttcatttc atcttccatt gctgcttcta gctctgcttg
     2281 tcgatccatc acttcattaa ggtccactgt attgacttga gcgatacgaa cgttggaact
     2341 gcccattggg aaactttttg catccaaatc aagaagaacc gcagctgatt tatcacttag
     2401 atttgttcct gctttcaaca tttcaagtcc ataagtgttt agatcgactt cagcgattgc
     2461 tgcaagttct tttgcagctg cttcatcttc aggcgtacat gttggtgatt tgaacaacaa
     2521 tgtatctgaa atgatagctg agagcatgat tcctgcgatt tctttaggaa ttgttacttg
     2581 gttttcttta tatagtttta aaataatcgt acttgtacat ccaactggtt ctgcacgata
     2641 gtacaatgga ttagctgttt caaagtttgc aatacgatgg tggtctacta cagctaaaat
     2701 atttacttcg ctgatatctg aaatactttg ttggaattca ttatgatcca caagcatgac
     2761 atctgcattt tctgctttag ctgactctac tactcgtggt gcagatagct gaaagtggtc
     2821 taatgcgtat tgtgtttctt cattaggtgt acctagagca actgcttctg tttctttacc
     2881 taattcattt tgcaagtgtg aaaacgcaat ggctgccccg attgcatctg tatctggatt
     2941 ttggtgacca aaaactaaaa tttttgacat gtcattcgct cctttgtgat ttctcctatt
     3001 taataatagc aaaaaaacgg aaagaagcaa tacttatctt ctttccgatg cgacctatcg
     3061 cgttaaatat tttgtatact cttctgtatg aagcaatcgt ttagcatttt cgacacgttc
     3121 ttttgtaggt ggttcgatcc cttctaatgg atatttcaaa ccaagtgttt cccacttgta
     3181 ttttcccatc gtatgataag gaagaatctc tactctgtct acatttgaca aggtttgaat
     3241 aaagttgtct agtcggatca aatattcatc ataatcactt cgctcaggaa ctagtacatg
     3301 gcgaatccaa accggtttgt tgatttttga caaatattga gccatttcta aaatattttc
     3361 attgtcccaa tgcgttagtt ttttatgctc ttcattatcg atgtgtttga tgtcaaataa
     3421 cagtaaatct gtatacttca tcaattcttg aaaacgtgaa aagaaaggtt cttcatacgt
     3481 aaaaggtttt ccgcatgtat ccaatgtagt atgcatcttc cgctcttttg cttttttaaa
     3541 tagttcgatc aaaaaatcga tctgcaatag aggttctcca ccactaacag taataccacc
     3601 tttatctccc cagtaaggtc tgaagcgttc tgctttatct aaaagttcat ctgctgtgta
     3661 ttctttccct ccgccaatat tccaagtatc tggattgtga caaaattggc aacgcatgcg
     3721 gcatccttgc ataaacacga caaagcgcag tccaggtccg tctactgaac cgaaagtttc
     3781 gatagaatga acatagccga ttgttttttc ttccatttaa gttcacatct tttctttgat
     3841 ttcattttaa gtacaagtta gctgtcgaag cgaagctttc tgaacaaaat ttttcgcaat
     3901 tgccgcttcg ttatgtgtaa aatttacagg gacggaacat acaagtcctg cccctgtaaa
     3961 tcgtttcttt caaataagca tactgattat tcgattttga tgcgatggct attcaacgtc
     4021 ctttcgacgc taagtcgaaa aacttttttc acgaactttt tacattctgt cgtgtgaagt
     4081 tctcatcaag acatctaatt gttgttctct tgtcaagtca cggaatttaa ctgcataacc
     4141 agatacccgg atcgtcaagt tcggatattt cattggattt tccattgcat cacgcaataa
     4201 atcattactg aatacattga cattcaaatg ataagctcct tttgagaagt atccatctaa
     4261 gacattacgc aagttttcga ttcttgtatc ttgttctttt ccaagacctg aaggattgat
     4321 tgtttgggta tttgaaattc catccaatgc acagccgtaa ttcattttag ctgttgagtt
     4381 caggctagct aataacccac tttgttccgc attgtaactt ggatttgctc ctggcgcaag
     4441 tggttttcca gcttcacggc catcaggagt atttcctgta tttttaccgt aaacaacatt
     4501 tgaagtaatc gtcaataatg aagttgttgg ttttgagttg cggtaagttt tatgacgttt
     4561 gatttgtccc atgaagtatt ccaagatcca tgaaactaat tcatctgcac ggtcatcatc
     4621 atttccgtat ttagggaagt cgccatccac ttcaaaatca actgctattc ctttttcatt
     4681 acggattgct tttacttttg catatttgat cgctgacaag ctgtctgccg catgagagat
     4741 cccagcaata cctgtagcaa atgtgcgttg caagtcagaa tccatcaaag ccagctgagc
     4801 tgcttcataa gaatatttat catgcatata atgaatgatg ttcaacgtat tgacatatag
     4861 ttctgctaac caatccatca tattcatgaa gttatgtttc acttcttcaa agtctagtat
     4921 ttctgatgtg attggacgat attctggtcc aacttgggcg ccagtcactt catcgactcc
     4981 gccattgatg gcatataata gtgttttagc caagttagca cgagcgccaa agaattgcat
     5041 gtcttttccg acaactgttc ctgatacaca gcaagcaatc gcacaatcat cagaacccca
     5101 ttgttcacga agctgattat cattttcgaa ttgaatcgaa gaactttcga tagcgattct
     5161 gctagcatat gttctgaatc cttctggaag gtgttcagaa tacaaaacag tcaagtttgg
     5221 ttctggtgaa ggtcccatat ttgttaatgt atgaagcaga cggaaatcat tctttgtaac
     5281 taatgaacga ccatctaatc ccatacctgc aatggataaa gtagcccaga tagggaatcc
     5341 tgagaataat tggttatatt caggtgtacg cgcgaatttg accatacgta atttcattac
     5401 taagtggtcg atcaattctt gtgcttcttt ttctgtgatt actccattat ccaaatcacg
     5461 ttggatatag atatcaagga atgctgagat acgtccgatt gacatcgcag caccattttg
     5521 tgatttgatt gctgctaaat atccgaagta tagccattgg acagcttctt ttgcatttgc
     5581 agctggttta gaaatatcat agccatattt cgcagccatt tctttcattt gcgctaatgc
     5641 acgatattgt tcagtgattt cttcacgtaa acgaatcact tcttcactca ttgtatgata
     5701 accagtcata tcatgatctt ttttcttttg ttccatcaaa tagtcgattc catacaatgc
     5761 cacacgacga tagtcaccga tgatacgtcc gcgaccgtaa gcgtctggaa gccctgtaat
     5821 gattttgttt ttacgagcag cacgcatttc agcagtataa gcgtcaaata caccttgatt
     5881 atgtgttttg cgccagtcag tgaaaatatg tgtcaattca tcgtttggtg tatagccatt
     5941 gctttttagc gcattgttag ccatattgat tccgccgaac ggcatgaatg cttgtttcaa
     6001 aggtttgtct gtctgtagac cgacgatttt ttccaaatct ttatccaaat agcctgcatc
     6061 gtgtgaagta atagttgata cgatttctgt gtccatatca tagacaccgt tacgctcgaa
     6121 ttggatatca tttaactttt gcaattcatc ccataatttt tctgttgctt ctgttgggcc
     6181 ttccaaaaag ctgtcatcac cgcgatattc agtgtagtta ttttgaataa agtctctagt
     6241 gtctacgcct tctttccatt tggtaccttt aaatccattc cattgctcca ttgtatggac
     6301 ctcctaaatt tctgttttat gtaacagttt ttacgtgatt agtataacac agcaccctat
     6361 ctttgcaagg gttttcttgt tttttattta acaattttta tgttttattt acatatattt
     6421 aaatcaacgt tgacatacca acatttaagc attgtgaaca ctttcatctt tttttgcaaa
     6481 cgtataagga tttattaaaa atgaatgaaa aagagacgcc acctgtatta atacagatgg
     6541 cgtctcttca ttaaaatact acaacagtta cgactcgatg aaaatctcag gagcatcgat
     6601 aacttcgatg acttctccac cttttttctc atccatcgca aatgatccat tcgatgttcg
     6661 atcacctata gggaaatcag ttgggtcaat cgttaactgc tgcccttttt gattcatcaa
     6721 ggttatttct tttgatcttc cttccgacat atagataata cggtgcggat tttttttcag
     6781 ctcacgcatg accgtcactc cccgcttcgc acgtgaagtt tggctgattt cttgagcaag
     6841 catgcgttta acagctcctc gctgagtaac gattacgatc ggcgtatcac cttctgctaa
     6901 cacaagcagc ccgttgataa tcctatctcc atctttcaaa ttcatagact tcactccagc
     6961 tgcctttgct ccaacaacgg gaacatctgt taaaggatag cgcaaaccca ttccttgatg
     7021 cgttactaag aaaacatcta agcctggctg atcttctttc agatacacag caatcagttc
     7081 atcttcttcc gcctttagct tcatacagct gagcggacgg ctcttatagg tgcgccatgg
     7141 ttcgaactct gtcattcgtg tttgcttgat caatccattc ttgctgatga atacaaatgt
     7201 tttctctgct tgaatttttt gatatgggaa aacagctaaa atcgattcgt ctacagacag
     7261 attggagatt gtttgagaaa tgtgctcacc agcatctttc catttcaaat cgggtagttc
     7321 atggacaggg cggtagatca catttgcttt atttgtgatc agaagaacat ggtccaatgt
     7381 actcaattca cctgagtata gcaagtagtc gccttctcgc atcccgattt cttctggttt
     7441 ggaagcacta taagaacgaa ggcttgttcg cttcacatag ccttctcgcg tcacactgac
     7501 aattacgtct tcttgtgcca ctaagacttg cgtatcaatt ttgatttctt cgatttcgtc
     7561 ttcgattgtc gttagacgag cggaagaata attttttttc acttctcgca attctttttt
     7621 cataacagag aaaagctctt tgtcatttga aaggatttta ttcaactctg tgatttgcgc
     7681 aatcaagctt tcggattcct tttgaagctc tgtgatatcc gtgttcgtca aacggtataa
     7741 ttgcaatgtg acaattgctt ctgcttgttc ttctgtaaat tgaaagacgt cgactaaatt
     7801 ctttttggcg tcttttttgt ctttactttc acgaatcgtt gcgattactt catccagaat
     7861 cgataaagct ttcatcaatc cttctacgat atgctgtctt ttttgagctt tggctaaatc
     7921 gaattgacta cgttttgtta tgacttgctt tcgatgctcg atataacttc ttaaaatgtc
     7981 tttcagtcca acttgatgcg gagtcatatg gtcgatagcg accatattga aattgtaatt
     8041 gatctgtagc tcagtgtttt tgaacaaata attcaaaatt ccttgagcat tggcgtcttt
     8101 tttcaattcg acgacaattt gcagacctgt tctatcactt tcatctcgta cttcagctat
     8161 tccatcgatt ttcttattca aacggatctc atccattttt ttcaccaagg tcgctttatt
     8221 tacttcatat gggatttctg tgatcacgat ttgctgttta ttacccttca ttgcctctat
     8281 ctttgttttc gaacgaagaa taacttttcc tcgtcctgtt tcataagctt ttttgatttc
     8341 atcttttcct tgcaagatcc ctcctgttgg aaagtcaggt ccaggaatat attccatcaa
     8401 tttttcaagg ctcgcttgtg gatgatcgat catatagatt gtgccatcga tcacttcagc
     8461 taagttatga gtcgggattt ctgttgcata tccagcagat atacctgtcg atccattgac
     8521 gagaaggttt gggtatcgag ctggaaggac tgttggttct ttttctgtat catcaaaatt
     8581 ccacacgaga tcgactgttt ctttctcgat atctgctagc atctctccac ttaacttgga
     8641 taatctggct tcagtataac gcatcgcagc aggtggatca ccgtccatac tcccgttatt
     8701 cccgtgcatt tcgatcagta cttcccgcaa cttccagtct tggctcatgc ggaccatcgc
     8761 ttcataaata ctgatgtcac catgaggatg ataattcccc atgatatttc cgacagattt
     8821 tgctgattta cggaatcctt tgtcatacgt attgccatct ttgttcatgg caaataatat
     8881 ccggcgttgc actggcttca atccatcacg gatatcaggc agtgcacgtt cttggataat
     8941 atatttggaa taacgtccga agcggtcacc catcacttct tctaaagtta attcttgtac
     9001 ttcttgtcga ttttccatca ggtgacctcc tattctactt caaataactg attgttttca
     9061 tttttatctg ctcgttcttc atctaataaa tcattagaaa ctgaaggaga gatttcagta
     9121 tcctcttttt tatctaagat actgccatct tcttcgagcg taaattgcac gtgattttcg
     9181 atccattttc ttcgaggttc tactttgtca cccatcagtg tggttactcg acgttcagct
     9241 tgcgcagcat catcgatacg gacgcggatc aacgtacggg aagtcggatc catcgttgtt
     9301 tcccacaatt gttccgcatt catctctcca agtcctttat atcgctgcag catatatcct
     9361 ttgccgactt ttttaatcat tgctgccaat tcgtcatctg tccacgcata ttcaatgact
     9421 tgcttctttc cttgtccttt ggatacttta tataaaggcg gtaacgcgat atatactttc
     9481 ccagcttcaa tcagcggttt catataacga taaaagaaag taagcagtaa tacttgaata
     9541 tgggcgccgt ctgtatccgc atcggtcatg atgattactt tgtcgtaatt acagtcttcg
     9601 atagaaaact caggaccgac tcctgcacca atcgtataga tcatcgtatt gatttcttca
     9661 tttttcaaga tatcttgcat tttagctttt tcggtattga tgacttttcc tcgtaaaggt
     9721 agaattgctt ggaactttct gtctcttcct tgtttagcag atccgccagc agaatcccct
     9781 tcgactaaat acaattcatt tttcttaggg tttcgggatt gtgcgggtgt caattttcct
     9841 gaaagaagag attcgccttt tttacgcttc ttcccatttc ggctttcttc tctggcttta
     9901 cgtgctgctt ctctcgcttc tctggctttg attgctttac ggaccaacat ctggctcatt
     9961 tcgctatttt cctgcaaata aaagcccatt tgttcactga tgacgttatc tactacggta
    10021 cgagcaactg gcgtaccaag tttttctttt gtttgccctt caaattgcag aagattttca
    10081 ggtacacgga tagaaagtac agcagctaac ccttctctga aatcactacc ttccagattc
    10141 ttgtcccgtt ctttcagcaa accaactttt ctcgcatatt cattataaga tttcgtcatc
    10201 gccgctttca taccagcttc atgggttccc ccatcttttg tccgaacgtt attgacaaaa
    10261 gataaaacat tttctgaata accgtcattg tattgataag cgacctctac ttcgatacct
    10321 tctttttctc ctgaaaaata aacaactggt gtcaatgtat ctttttcttc attcagataa
    10381 tcaacaaatt ctttgattcc ttcttcgtaa tgaaaaattt ccttgactgg ttcttctcca
    10441 cgaagatcag aaagagagat tttaacgcct tttagaagaa aggcagattc tcttaaccgt
    10501 tcggctaaaa tttcatatga aaatttcgtt gtactgaata ttgtatcatc tggtaaaaaa
    10561 tggacagtcg tcccgtttgc tttttttgtt tttcctatct ttttcaaagt accgtcaggt
    10621 ttaccgccgt ttttaaattt ctgctgatac tctacgccat ctctaacgat cgttacaatt
    10681 agccatttcg ataaggcatt taccacactg gctcctacac cgtgcagtcc accagaagtt
    10741 ttgtaccctc cttgaccaaa ctttccgcca gcatgcagca cagtaaaaat cacttcaact
    10801 gttggtattc cggaagcatg cattcctaca ggcatcccac gtccgctatc tgcaacagtt
    10861 atactattgt cttcatggat ggtcacatca atctcactgc cataacctga taatgcttcg
    10921 tcaactgcat tatcgacgat ttcatagact aaatgatgca agcctcggct gtcagtggat
    10981 ccaatgtaca tccctggtct ttttctaact gcctctaatc cttccaatac ttggatggac
    11041 gagtcattgt attcgttgtt tacttttttt gccaaggcaa acgctccttc aataatactt
    11101 tgcggcaaaa ccgcaccacg attatcatac tagaaaaggt cgtgaaaaaa aagagggtag
    11161 acgagaaatc aattttaatt atctcctaac cgctttttcg acattcccct ctatcgatca
    11221 tctcttcttt gtggtcattt ttatttttgg ttttctttca acttaccaag ttcaatctta
    11281 atacaacgat ccattacgat atctgtacgt cctgcttttc ttagcatctc agctgcttct
    11341 ttgttttcaa tccctaattg tgcccagaac actttagcat cagttttcaa aaaatcacct
    11401 gctatttcag gtaaaaattc actccgtcta aatacatcta cgatatcgat tggatcatcg
    11461 atttctgtca aattcgcata gactttctcg cctagaattt cttcaccagc caataatgga
    11521 tttactggta tgattttata tccattgtct tgcaatacct gtgcaaccat gtaacttgtt
    11581 cgttctggtt tattgctcaa gcctacgacg gcaatatttt ttgcttcttc caaatagttg
    11641 aaaatcgttt cttgtgttgg attttttaca gacatttctg ccaccccttg cttttctttt
    11701 tattccatct attatacttg catctttcat tttttcaatt gttcctgtta cctattcatg
    11761 ctaaaataaa ggatagtttt caacttgaaa gagggtaaaa aatgaaattg atcttattac
    11821 ttattttagc atatctttta ggttctattc cttccggtgt ctggattggc aaattgtttt
    11881 tcaaaaaaga tatccgacaa cacgggagcg gtaatactgg aacgaccaat acatttcgtg
    11941 tattaggcaa aacagccggg attgttgtcc tgcttatgga tattttaaaa ggtacattgg
    12001 ctacttgttt gccgatgatc ttccaccttt ctgggattaa ccctctttgg tttggggtat
    12061 gcgccattct tggccataca tttccgattt ttgctgggtt caaaggagga aaagctgttg
    12121 cgacaagtgc aggaatgctt ttaggactta atcctgtatt ttttgtttat tcttgtatca
    12181 tctttatcgt tagtttattt tgtacaagta tggtgagttt gagcagtatg atcagtgcag
    12241 tattgatcac tttatctacg atcgtccttc cttatgtagc tcctgtgatt ttggctaagc
    12301 ccaattggtt actgacgatc attgcctttt tagtttcttc ctttatcttt tatcggcata
    12361 aagacaatat caagcgtatc agaaaccata ctgaaagtcg aatcccgttt ggtttgaata
    12421 gcagcaagaa aaaatcctga tactttttca ttccaaagag ataaaacggg agtgtgacaa
    12481 atcgatgtca cactccctta ttacttacag gatagctttt ttacacacct tggtaaacgg
    12541 tgttccctgc taacttctcg gtcacaacct cttacttcac tgtgatggaa tattttgtct
    12601 tgaagatttc atgggcacct agttgattga tccctacttt ttccatcaag tttccactac
    12661 tgttcaccgt gtcagcgatt ccgcaccacg gttcgataca aacgaaaggc gcttcttttg
    12721 gataaggaga ccagatccct acataaggca tatctttata gcttaaagtt acgctatggg
    12781 aagatttttc acttcgaatg gcgtacgcat ttagacccct tgtttcaaaa accaaggcat
    12841 cttgctcaaa aagttcacga ttcaaagcca gattcgtatt tgtctgacct aacgtctttt
    12901 gatccatatc gatgaatggg cctttcaatg gtaggcgtaa tcttgatttc aatggagaaa
    12961 atgccagata gaaatcttca aatgacaatc cttcttcaag aggtacattg aaagcaggat
    13021 gtccaccgat agaaaagaac atttcttttt caccggtatt tgccacttga taatgaaccg
    13081 tgatgccgtc tcctcctaat tcatagctga tcactaattc aaaatcaaat ggatactttt
    13141 ctaatgtctc cggcgtactc cttaagatga aactagccat ttcttgtcca tgttcaatga
    13201 cttcaaaatc catatcccga gcaaatccgt gttgccccat ttgatatgtt ttcccttgat
    13261 aaacatactg atcatctttt aaacgtccaa caaaagggaa taaaaccggt gcatgtctgc
    13321 cccaatacgc tggatcacct tgccagatat actctaaacc atgttctttt gatttcagac
    13381 tgatcaattc cgcaccgtgt tcagcgattt cggcgatcag ctggtcattt tgaataataa
    13441 cagtcattgc cattcctcca acttcgactt actttttaaa atgggaaggc tggaacaaac
    13501 atttctatct gccccagtcc ccttacttct aataactgtg ttttgctaac tcgtctttaa
    13561 aacgggaatt caatactttt aaataagttc ccttcatgcc taacgaacga gattcaatga
    13621 ttccagctga ttccaatttt cgtaaagcat tgacgatcac agaacgagtg atgccgatac
    13681 tatcggcaat attagaagca gtcagacgac cttcatctcc atccaatgct tcaaagatag
    13741 cttgtacagc cttcaattcg ctataagata atgtgttgat tgccatttgg acagccgttg
    13801 cactccgtac atctgcttcg atgcttcgag acttctgata aaggatctgc ataccgacaa
    13861 ccgtagctcc atattctgct aagactagat cgtcatctgc aaacgattgt tccatacgag
    13921 ataagataat cgtacccaaa cgttcgccag ctccaaagat cggaacgaca gtcgtcagac
    13981 caaatggata tttctctctt gactctaccg gaaatgcagt aatatcacta gtaattggga
    14041 tattagcttc agttttcatc aactgtgcca ctgtatcagt ataagattca ggaaattgtt
    14101 tctcaacaaa cattgttttg attcgttcat tgttcacatc gtgacgttca ttataaccta
    14161 gtaatactcc gtcacttcct atgatatagg tgttgctgtc aagaatacca cctagtgtga
    14221 cagccatttt atcgtaaggc aaatctgctt gcatgtcaaa agtattcttt tgctgcaaaa
    14281 gtgtgtttat cttgcgggtt ttcgttagca agtctgtcat tgtcgtcctc cgatttttta
    14341 taaaatatag cgacttaaat cttcatttgc cgcaattgag ccaagtttgt catccacata
    14401 tgcttctgtg atcgttattt ctcccatttg catatctggt gcttcaaata atagatcttc
    14461 tagtagtttt tccaaaatgg tatgcagtct gcgtgcgccg atattatctg tgtcacggtt
    14521 gacatcgaaa gcgatttgag cgatccgttc aattgcttct tttgtaaacg taactgacac
    14581 attttctgta ccgatcaacg cgacgtattg ttttactaaa gcattatttg gttcggtcag
    14641 aatcttcaca aaatcttcag ctgtcaaatc atctaattct acccgtatcg ggaagcgtcc
    14701 ttgaagttct ggaattagat cacttggttt agacaaatgg aaagctcctg aagcaatgaa
    14761 taggacgtga tctgtttgga tgatcccgta ttttgtgttt acttgtgaac cttcgacaat
    14821 tggcaatatg tctctttgga cgccttctct ggatacttct ccagattgct gtgatttaga
    14881 agtgatctta tcgatttcat cgataaaaat aattccactt tcttccgcta atttgattgc
    14941 ctcactatgg atatcagcat ccttgactaa ttttgctgat tcttccttga tcaataactc
    15001 taaagcttct ttgactgtta ccgtccgttc cacttttttc ttcggtgtca aagcacctag
    15061 agtttcattt aggtcgatcc ccatttgctc caatccattg ttcatcattg gtgcaggttt
    15121 cttttggtct tcaacttcaa tggttacttc acgactatcc agtaaaccct tttgtagttg
    15181 ttccaagact gtacggcgat tgattcgaat atcttctgtc acttcttctt gcggttcagc
    15241 ggtattttgc tgactagcat taaaaagatt catcatttgt tcaaattgat tagtggattg
    15301 tttctgttct tttttgatac caggtaccaa gatttttacc aaacgttttt ctgcattttt
    15361 tttagcactt gtgtatacac ggctgtactg ctctttttct acgatttgga tcgcattttc
    15421 tactaaatcc cgaaccattg attctacatc tcttccgaca taaccgactt cagtaaattt
    15481 tgttgcttcg actttgacaa atggtgcatt gacgattttt gccaaacgtc gtgcgatttc
    15541 tgttttaccg acccctgtag gtccgatcat cagcatattt tttggtgtaa tatcttgttg
    15601 cattttttca tctaactgca aacggcgata gcggttacgc aaagcaacgg caacagattt
    15661 ttttgctgcg tgctgtccta cgatatattc atctaattca cttacgattt gtcttggtgt
    15721 ttgttttaat tcatacatat ctactcacct cttacagttc ttctacgata atattatgat
    15781 tagtaaagat acagatatct gcggcaatat ttaacgcatt ttctgcaatt tctttcgcac
    15841 tcatgtcgtt ttgagcatag tttttcattg ctcgggctgc agccaacgca aaattccctc
    15901 ctgatccgat agctaagata ccatcatctg gtgtgataac ttctcctgta cctgaaacaa
    15961 gaagcatttc tttatcattc attacgatca gcatcgcttc aagtttttgc atagattgct
    16021 gtgtccgcca ttcttgagct aattcgacag ctgcgcgttg tagattcccg ttatattcgt
    16081 ttagtttgcc ttcaaatttt tcttctaaag taaacgcatc tgcaacactt ccggcgaaac
    16141 ctacaacaac ttctccattg tagatacgtc gtactttctt tgcagtgcct ttcatgacaa
    16201 cagattcacc cattgttact tggccatctc cagccatagc aaatttcccg tctttttcga
    16261 cggcacagat agtggttgaa tgaaatggtg attcgaccat gtttgtttcc tcctaaattt
    16321 gttcttttcc ttatttttac gctcttggat ggaaagaacg atagtttttt tgtaaacttt
    16381 cttttgttac atgggcatag atctgggtag tcgataaatt ggcatgtcct agcaattctt
    16441 ggactgtccg caaatctgcc ccattattta ataaatgagt agcaaatgta tggcgcagca
    16501 tgtgaggatg gatatcacta tccaaactgc tttttttgat catttgattc aagacatatt
    16561 caattccagt aggcgtgatt ggttttccat gatgattcac aaaaacgata tcatgctctt
    16621 cgtgatattt tgtcatcaat acctggcggc cttccctgaa atattcttgc agagcatctt
    16681 gagcaaaaga acctagagga gcatatctct ctttgttccc tttaccatgg atcaacatga
    16741 cctctgaatc aaaatcaatg tctgataccg acaaactgct acactcactt aaacggatgc
    16801 ctgacccgta aaggacttcc agtaaagcgc ggttcctcaa atccaaagga ctatctcctg
    16861 ctacgctgtc aaaaagtgct tgcatttcat tttcataaaa aaaacgtggc agtctcaaat
    16921 ttttcttttt gagatgaacg taagaaaatg gattttcttt tatcacttct tctttgagaa
    16981 gatactgata aaacgaacgt aggctggcta tcttgcgact gatagaattt ctgctatagc
    17041 taggacgatg atcagctaat gcactcaaat agacacgaac cgtaaaatat tccgccttca
    17101 gcagatcagc gtctccagat tcctccaaaa aatgtttgaa atggagaatg tcttcttcat
    17161 atgcttcttt cgttttttct gaatatcctc tttcgacaat caaataacgc aaaaattttt
    17221 gttcccacgt ggcttctgtc attttctcac cttccttttg tcccagagaa taatttagca
    17281 cagcgtttat aaaaagacaa ataaattgtc agaatttaac caatatttct aagtttgttc
    17341 acaattggcg caattcctca cattttgttt gagaaattct taagaaaaac gcaagaaaaa
    17401 gagcgagact atccgtctca ccctcttcat ggagtttcct tactcatttg ggattgcagt
    17461 tttttctaat tctgttttta ttgcctcatt agcttttaaa gctcgttgtg caattgcttc
    17521 atatcttgct tttttgtctc gaatgcgctc tggcagttca ggaaacagac catagttggc
    17581 attcatcggt tggaaatgtt ttccttccgc atgagtaatg tagtaagcca tacttccaat
    17641 agctgtttct tgtggaaact cgactggttc catttctttt gctaagcgtg cggcgttgat
    17701 tccagcaagt agtccgctcg ctgcactttc aacatagcct tctacaccag tcatctgacc
    17761 tgcaaagaac agatcttcgc gtttcgttga ttggtaagtc ggtttcagca attctggcga
    17821 attcataaag ctgtttcggt gcatcacacc atagcgaacg aattcagcat tttctaaccc
    17881 aggaatcatc cggaaaacac gtttttgctc gccccatttc aagtgagtct gaaagcccac
    17941 gatgttgtat aaagaagctg cagcattgtc ttgacgcagc tggataacag cataaggacg
    18001 tttgccagtt ttaggatctt ccaatccaac tggtttcata ggtccgaaaa gcatcgtttt
    18061 gaatcctcgc tgtgccatta cttcaattgg catacatcct tcaaaatatt tttctttctc
    18121 aaaagattta agttctgcca cttcggcatt gattagtgcc tcatggaaag ctttgaattc
    18181 ctcttctgtc atcggacagt tcaagtaagc tgcttctcct ttgtcatagc gggacttcaa
    18241 atatacttta tccatatcga tcgtagcttt gtctacgatt ggtgcagctg catcatagaa
    18301 ataaaaacca tctgaaccat tgaatgcttt gatgctttct gctaatggat gagaagtcaa
    18361 aggtcctgtt gctactacgg tgattccttc cggtatttca gtaatttctt cgtttatcac
    18421 tgttactaat ggatgctctt tgacttttcg ggtaattgtt tcagaaaaag aatcgcggtc
    18481 aacagctaag gcaccaccag ctggcactgc tgtttcatct gccgaagtga ttactaccga
    18541 attcaatcgg cgcatttctt ctttcaataa ccctactgca ttagccaaac tgtttcctct
    18601 taatgaattg gaacatacta gttcagcaaa attacttgtg tgatgcgcct cagtcgattt
    18661 gactggacgc atttcatata aacggacagg gactcctgct tgtgcgatct gccaagcagc
    18721 ttcacttccg gcaagccctg ctccaacaac atttaccatt ttcaatgaaa gaacctcttt
    18781 tctattattt ttggatgtct tcttcgtaat ctccatttac acagactact tgtttaccgc
    18841 ctttgacttt cttctcgacc agatatccgt cacattttgg acaatttctg cccactggtt
    18901 tatcccaaga agtgaattca cattctggga aacgggaaca accatagaat agtctgtttt
    18961 tcttagattt tcgttcaatg acttgtcctt catgacatac tgggcaagtc acaccgattt
    19021 cttttacgat tggtttcgta ttccgacaat caggaaaatt actgcaagca taaaacttcc
    19081 catatttccc taattttatc accatcggat gtccacagac atcacaatcg aatcctgctg
    19141 gttcatcttt gatttgaatt ttttcgattt tttcttccgc attcgtcagt tctttttcaa
    19201 acggtttata gaaacgatca accactttga cccacgcttc cgtgccttct tcaattttat
    19261 ccaaatctcc ttccatttct gctgtgaaat ggatatcgac gatttgaggg aagaattcac
    19321 agattaatga attgacaatt tctcctagtt cagtcggctc aaatcttttt tgtgtcaatt
    19381 ttacatagta gcgtctttgg atcgtatcaa gtgttggtgc ataagtggaa ggtcgcccta
    19441 ccccgttttc ttctagtgca cgaatcaacg tagcctcact aaaacgtgct ggtggttgag
    19501 taaaatgttg ttttggttca atatcaacag attggactac atcgccttct tcaagttctg
    19561 gaagaatatt ttctttctct tctttgccgt catctctacc ttccacatac acttgcatga
    19621 atcctttgaa tttgattttt gaaccgttcg caatgaagat gacaccattt tgttctaatg
    19681 tcactttcat tgtatctaaa atagctggtg tcatctgact agccacaaaa cgtgaccaga
    19741 tcaatgtata aagtttcaat tgatctttat ctaaatattg tttgagttca tctggtgtac
    19801 gaagcacact cgttggacgc acagcttcat gggcatcttg tgcgccttga gcatttttga
    19861 tctttcggtg atcatgtcta gaaaattctt ttccatacgt atcttcaata aatgctgcgg
    19921 cctctgcttt tgctgtatcg gcaatccggg tagaatcagt acgcatgtat gtaatcaagc
    19981 cgactgttcc ttgtttaccc aaagtgattc cttcatataa ttgctgtgct accatcatgg
    20041 tttttcttgt tcgaaagttt aacttacggg cagcttcttg ttgcaggcta cttgtagtga
    20101 aaggattcgc agggtttctt ttgcgctctt tcttttctac ttttttaact tcgaagtctg
    20161 ttccatcaat tctagcagtg acttctttta cactttgtgc atctggtaat ttctttttct
    20221 taccgtcgat tccccagaaa tttgctttga atttcttgcg agctttttta aaattaccgt
    20281 cgatgctcca atattcttct ggaacaaatt ctcggatctc tttttcacgg tcaataataa
    20341 tttttaaagc gacagattgg acacgtcctg cacttaaccc tttcttgacc ttacgccaga
    20401 gaataggact gatcgaataa ccaacgattc tgtctaaggt acgacgagct tgctgtgcat
    20461 ctactaaatc tacatcgatc gttcttggtt ccttaaaagc tgctttgact gcgtcttttg
    20521 tgatttcatt gaagaccacc cgattttttt ctttcagatc caacccaagt aggtacgcta
    20581 aatgccaagc aatcgcttct ccttctctat ccgggtcact tgccaagtaa actttttgag
    20641 ctttttttgc tgcggctttc aggcttttga tgacatcgcc ttttccgcgt atagaaatat
    20701 aatggggttc ataattgttt tcaaaatcga tacccatttt acttttcggt aaatctctta
    20761 tatggccaac actggccatt actttatagt ttcgtccaag atatttctca attgtcttgg
    20821 cttttgcagg tgattctaca atcactaaat atttatacgc cattactgaa tggactcctt
    20881 tccattgtcg tgttcctact attaaaagag gattcataca aatcgtagct aatcatatcc
    20941 attcagaaaa cgtttgtcaa gaaaagcaaa tgaaaagtat ggtgttattc tttaaattct
    21001 gccaagatat cttcacccga aaccgtgcaa actgcaccgt cttggatcaa atgatgacat
    21061 ccggtagaat gttctgttaa aatatttcct ggaacagaaa aaactgttct tccaagatcc
    21121 aatgcttgct gtgctgttat cagagagccg ctttttttct tggcttcaaa gacacatgtt
    21181 cctttagaca aagctgcgat aatccgattt ctcattggaa aacaatgctt tttcgggccg
    21241 gattgaggag gatattcact caaaagaaga tgatcatttg caatcacctc ttgcaagtca
    21301 cgattttcag caggatagac ttgctctatc cctgttccaa tgactgcgat tgtccgtcca
    21361 ccatgagaga ttgctgcttg gtgaccgtat gtatctatcc ctttcgcgca gccactgacg
    21421 atcacatatc cagcttctat cactggtttt agtaaataat aaactgtttt tttggctaaa
    21481 gcagaagtgt gtcttccacc aacaacgctt aacatgttac aagaaagtaa agatagatca
    21541 ccattataaa acagtacaat tggcggatag gtcaagtgaa gcagctgagg gggatatttg
    21601 ggatcaaagc aagtgataaa cgactgtttc gattggattt tgccccagtt agcgtgaatt
    21661 tcctgccaac ttttgcgaaa cagctcccgg tgtttggtta ttttacctat cttaatgatg
    21721 tcttcaaatc cgatttcttt ataaggatac tgcatcatcc agttagcaag tgcccatttt
    21781 ccttgactac tgattccttt tgtataagca agtttcaata aattttcttc tatttgatac
    21841 atcataaaac cgcctttcgt ctctctttct aataagatac gtaaaagcgg ctgtttttta
    21901 ttgtaaatat ttttttacag gtgcaaaact tttacgatgg attggcgtaa ttccttgtcg
    21961 ttcaagtcct tctaagtgtt cctttgttcc ataacctgca ttatgcgaga aaccatatcc
    22021 aggataaagc acgtcatatt ctttcatcaa ttggtctcga tatactttag caatgatact
    22081 tgcagcagca atcgacacag atcgtgcatc ccctttgatt attttttcct gctgaatagg
    22141 caaatccaag gtcatcgcat cgatcaacaa atagcttggt acaatggaca atttttctac
    22201 tgcttggatc atcgctaatt tacttgcttc ataaatattc acatcatcga ttgtctgctg
    22261 atcaacgatc ccgatcccga ttgccagggc tttttcttga acttgttgat aaagaagttc
    22321 tcgctttttc tctgataatt gtttggagtc attcagccct aatatgatct catttttcgg
    22381 caagataact gcagctgcca cgacaggtcc agctaatggt cctcttccta cttcatcaat
    22441 gccggcaatc cattggtgac cttgtgcgta cccttgcttt tcaaagcaaa acatttcttc
    22501 atatttttcc ttaattaatg cttccttttt caatcgtcgt tcaaaagaag ccagtgcttg
    22561 ctgcacgcct ttgcgtggat catttcttaa tgattctagt gtaggatcat ctattctctg
    22621 tatgcttttc aatcgttctt tgatactttg gatggattca gtcatcactt ttttgctcca
    22681 tttcttcgca acgatccagt gtgtatcgtc caagttttcc ttgacgaata tcatgcacga
    22741 ctaattcgct gccacgatca tagtcttctt tatacccacg ttttgctgta atatccatca
    22801 gtaattccgg tgccaatttt tcttcttgct cgattgataa accataacga tcgctgattc
    22861 ttcctgaata atatcgggca aaaaagtcta atccgtaaat cgctaaatca tctaaatgga
    22921 caagctggtc tttgatcgcg cctgtcagcg ctagtttttt gccgatttct tcatcttcaa
    22981 atttgggcca taaaatccct ggtgtgtcta acaattccaa ctcattacct aaacgaagcc
    23041 actgctgacc ttttgttacg ccaggcttat ttcctgtctg tgcaattttc ttgcctacta
    23101 gtcgattcat caacgtagat ttcccaacat ttggaatacc taagaccatt gcccgaatag
    23161 cgcgaggctt gatcccccga gcagcatctc ttgcccgttt ttctttcaat gcttctttag
    23221 ccgcaggtac tagaccttta attcctttac tttcttgtgc cgtgatagct aatgcaagat
    23281 ggccttgttt acgaaaatat tcttgccaca gctttgtctg ttgtggatct gccagatccg
    23341 ctttattcaa taaaacaagg cgaggttttt gctgaatgat ctgatctagc attggattac
    23401 gagaagataa aggtaatcgt gcgtctacca attcaaatac gatgtccaca tatttcattt
    23461 tttcagaaac ttctcgtcgg gcttttgcca tatgtcccgg aaaccattgg atggtcattg
    23521 ttctctccat tcctttcata ccggaatatt ccccggcttt acttgttttt ttctttttca
    23581 atataaattc acttttttat tataaagaaa ttccatctat tagttaagtg aaagtgaacc
    23641 agtcctcatt ctatcacatc ttcacttttt tgatgatgca aatttttatt ttggtttcta
    23701 tatatgaatc taaatttttg acagcgtttc ctaaataaga gatgatagac gaacaaaaca
    23761 gaaaggagac gaaacaaatg aatagtatgg tttttgttaa ttttcctgtt gctgatgtca
    23821 atcgttccgt tgctttttat gaaaagctgg gttttaaaaa aaatgaagaa ttttctactg
    23881 aagaagctgg ttcaatggta tgggatgaca ctttttggat aatgcttctc tctcatgatt
    23941 tttataaaaa atttataaaa gataaagaaa tagctgataa taaaaaaaca agtggtgctt
    24001 tgatttcgtt tagtgttgaa aatgcggatg aagtaaaaaa atttgctcaa attgcaaaag
    24061 aaaatggtgg tactttccac gaagtagata tgggaatgcc tgaagaagaa atgttttcat
    24121 tagaagtcca agatctagac ggaaatacat tagaacccgt atggatgaag atgtaagcag
    24181 ataactaatc tgacgttttt tataacaaaa aaacaaaggc ggatgatacg ataagtattc
    24241 tccgcctcta ttttttttat tcgtacaatc tcttttttac agtttataaa agcagtcttc
    24301 tcaccctttt tttaattgtt agatgccagt gtatgtaaat caccaatgtg tcatgacaca
    24361 ttggtgattt tatggtatcc ttttattatt aactgacaaa ggagagaata ttttgaacac
    24421 tttgattgca aaagacgttt ttagattgcc cgaaatgaat gcaagattgt tgtcgacctc
    24481 tgctctttta ttagcccttg atgtcatctt atacaagata gcgattggtc ctgcttattt
    24541 tcaaatcaca ttcggatttt taagtatggc gcttatgggt tatctatatg gaccgatctg
    24601 ggctggtttg cttgaaatgt tgtcgaacac tttaaatttc acgatatttg gttcaggaac
    24661 gtttcaagct gcttttctta tcacggcttt ccttggtgga tttattaatg gcttattttt
    24721 atatcataaa aagataaacc tgccttccgt catcgctgcc caattattga ttatgatccc
    24781 agtcagcctt tttatcaatt cttggctgat gtctgttttg tttggtgcag atttcaaagt
    24841 aatttttcta ggtagattca tcaggaatat gatccaaatc cctattcaaa taatggttgt
    24901 ttattcattt atcaaaactt tagaggctag aaaagtgttt aaacgagctg tttgatttta
    24961 cgtactaagg tagtaaactg attttggatt aaacaataaa gcgtgcagtc tcaacagact
    25021 gcacgcttta ttgtttctca ctggatccat tttatttcat caagtggata atagacaaat
    25081 cttgctttac ctagaatatc agttttattg atcgtgccaa acgaacgact atcttttgaa
    25141 actcttcgat tatctccaag tacaaaataa ttatcttccg gaagcttgga ttgtcccgtt
    25201 agctcttgta gtgtaaagtc cgtcgtatag gatgcatttg catgatctga tttcaggttt
    25261 ttagttagat aaggttcctt aactactttc ccattgatct tcaattgatc attttggtag
    25321 ctgacatttt ctcctggaag accgattact cgtttgatat aaatcgtgcc atctgctaat
    25381 tgaaaaacaa caatatcaaa tcgtctgatc tccgaaaatt tttccatcac aaccatatca
    25441 ccttgcttca acacgttttc cattgaattg ccttccactg gtaccgggat gaataaaaat
    25501 ccccgcaaca tgaacgcaag gaatacagaa acaagtaagt atttgaatac aagccagaag
    25561 cgattaatat agcgttgttt ttttgtcaat ctctatcaat ccatccctct ttttttcatt
    25621 ctttctttat tataagaaaa atatcaaaaa aatgcgacta gatagctgaa ataaaaataa
    25681 ttctttcaat taggctattg gacaaaaaac ttttttgatc ataaagtaca aaactatctc
    25741 ttatatgtca tgattatttt ctaaatattt ttcccatgct tcgtcaaaga tatccatgga
    25801 cggcaaatag tcgacattca gttccagata attagaaatt tcttcgtagc tagagctgtg
    25861 cttaggaaat tggatgtctt taccggcttc tgtagcgaaa acagataaag catcatgtgt
    25921 gccaccgcct cgcagtgtca ttaaataatg aaaaaagctt cgattcaccc ttcgttcctc
    25981 caattttctt cgataaaggc agcccggcgt gcatggtttc ttttaaaatt ttcaggatgc
    26041 tttttataat actcttgatg gtcttcttct gctagataaa atggttgggc tggttcgatt
    26101 tttgtaacga ttggctgttc gaaacgtcca ctttcagcta actgtttctt acttttttca
    26161 gcaatgatcc gctgttcttc tgtcaaataa aagatcaccg ggcgataatt gtcaccgcga
    26221 tcctcgaatt gtccgaaagc gtcagttgga tcagtttgct gccagtaaag ttctactaat
    26281 tgttcatatg aaatcaatgt tggatcatag ataatctcca ctgcttctgt atgtccagtc
    26341 gtttggcttt ttacttgttc ataagtcgga tctggtacat gccctccagt atatcctgac
    26401 atcactgtat ggataccagg aagcgtgtcg aaaggctgga tcatacacca gaagcagcct
    26461 cctgcaaata ttgctcgttc cattatgatt ccccctctaa tactactcat ccgatgattt
    26521 tttattttct tttgtcttgc tttcagtact gctggtgcta gttttgttgg tatttgagtt
    26581 gtttgaagca tcagaggtat cttttggcaa atatagacta aaccgaatgt catcatttac
    26641 taagtcgatt ttatctgctt taatatacat cccgttttgc attcggaatt gatccaacct
    26701 aagcatgata gtcagatcat ctgtattgac ttcgatccaa tctggtaatt tatagtttct
    26761 cttgatcata ttcatcacat ctttgattgg caaactcaat gttccgatag aaagacttct
    26821 agcttttagt tggacatttc cgttttccat cacatacgga tcaaaatata aatagaaatc
    26881 gataggaaaa cctagtactt cgaaagtacc attgagtagt gcttcatttt ccaaataaaa
    26941 tttatattca atatcactat ctttttgata ctcactaagg aaaaagctga tgatttgatt
    27001 gactttttct ttatttgaat tgatcgttaa gacaggatct ccttgtcttt ccgtaagagc
    27061 tggtatttct gggagttctg gttcccgatt agcaaaaata cggctgccta aaaatacggt
    27121 cgaaccaagt actaccccaa gaaggataag gaaggcgaat ttccagtaat ttgttttctt
    27181 tgttttgttt gaagatacgt cagatcttgt tttcatgttt attgatcatc ctccttctct
    27241 aaccattggt tttgtgtctc gatcagctga tctctcactg catttgccat caattgataa
    27301 cctaaattat ttggatggaa atgatcttct tcatagagca cattattttt gatgccattt
    27361 agattttctg tcgtgctagt tgtttgatct gttccttctt ctgtgacacc tacttcctga
    27421 ttcaatcctt gataaagcaa atcattgatt gggataaagt agcagttcgg catttttttt
    27481 gtcaccgttt ctgttccttt gttccaattg ttaacaattg tctgcatgtc agtgatctca
    27541 ggaaagttca aataaaatgg attgtagatg cctaaaatgt aaataggtgc ggttttattg
    27601 agggatcgaa attcttccaa tagttctgtg acattttcct gatagttttt cagtggcttt
    27661 ttaaaggaat tgaccgttaa cccgaataaa ttattttgga taactttcat caaatcatta
    27721 ccgccaacag tcagtgtgat aaaatcagct gttttgatat tgtttcgaat ttcttctttt
    27781 gtttttactc gctttaagat ctggtcactt cgttcaccat ttacaccata gttttctgtt
    27841 tcaaccgcgg tcaaattata acggtctttt agatcatttg cgacaagggg aacgaatcct
    27901 ccttgcttgg tttgatctcc tactccttca gtcagcgaat ctccgatagc tatgtagtga
    27961 agtttttctt ttttatttgg atcttctctc acagtagtta ctggtttcat tagtgggcca
    28021 gctttcggta tagaagcagc taaacctgca tagatgatga tcgcaattaa taatgggaac
    28081 aaaacgagga tcaaatgtcg tttcgttaat ttcatttttt cacctcttgt attcgattgg
    28141 ttctaatgtt atcaaaaatt ttatcaaaaa ggtagtcttt atgaaaaaag aaaacaatta
    28201 ttttcctaat tttataaaga aaaagagaat aagctgcaag cctttcaggc tgagggctta
    28261 tcctcttttc gactaatcta cataatacat gattgcaaac gcgtctttac cagtatgcgt
    28321 agcgataacc ggattcgtat gcagtacagg gatatgcatg tccgggaata attcttgcaa
    28381 accctctttg aattcttgcg ccaattcttt tgcatccgca tgtgaaatac cgatttgtcg
    28441 cacatttggc aattctttca gttcattttt caaattatta aaccatttat cgaacgtttt
    28501 ctttcctcgc cccttagtta cagggattaa ttcagaatgg gcgaaattca tcaccacgcg
    28561 tatgttcaaa acactagaca gcaaaccagt tgttcggctg attcttccac cttttaccaa
    28621 gttatctagt gtggatattc cgatgtataa ttttgtattg tcttgtactt ttttgacttc
    28681 ttcaaggatt ttctctactg ttttaccttc ttttgctaat tcagctgctc gaattacctg
    28741 aaaagataat ccttggtcag tggtatcact gtcgataacc gttactttcg tttttgttag
    28801 attactggct tgtcttgctg cttctactgt tccactcaag ccttttgtca tatggatcga
    28861 aatcacttca ctgccgtctt ctcctagctc atcatacatc gagatgaatt ctccaattgg
    28921 tggttggctt gttttgggca gattttttgc tttcgccatc atttccataa atctttcgcc
    28981 gtctagttgg tcatcatcgg gatatactgt tccgtcgatc atgatggata gcggcatgac
    29041 acgaatgcct aaagcatcgc gtacattttt ttccatcgta catgaagaat ctgttacgat
    29101 ttttacattt gtcattattt tctctctctc tttctttaac aagatgcctt attcatgtta
    29161 aactacatgt aagacaattt ctatttttag tataacagac aaataatgat ttttcattaa
    29221 ttacacctag taaaggagcc gttttcatgg aaaaaacgaa atttaatcga acgtatgaaa
    29281 tattaaatga agtgtttaat gcagtcaccc atggaattgg gactggattg agtatcgcgg
    29341 gactagtggt tcttttagta aaaggtgccc acttgcattc ctccctccat attgtctctt
    29401 atgctatcta tggatcgatg atgattcttt tatttttaac gtctactttg tttcattcat
    29461 tgatttttac aaaagcaaaa aaggtgttcc aagtattcga tcatagttcg atttttcttt
    29521 tgattgcagg aagttatact cctttttgtt tgctgagtat ccgaggatgg ctaggttgga
    29581 ctttatttgt attaatctgg ttgtttgcga ttcttggcat tatatataaa gcgttgacgt
    29641 tgcataaacg ggagagagta tcaaaagtat ctacgatcat ctatattttt atggggtggc
    29701 tctgtgtcgt ggctgctgtt cctctttatc actctcttgg aatgactggt gttattttga
    29761 tggtagcagg cgggctttct tacacgatag gcgcattctt ttatagcttg aattctatcc
    29821 gctatatgca tgttgtttgg catttgtttg ttatgttagg tgcaggtttt atgtacttct
    29881 ctgttttatt ctttacttaa taaaaaaaat aaagctatgg atgggacagc tctaaataac
    29941 agctagtcta tccatagctt ttttatatcg tcaattcgcc tgtcccgctt tttacaggat
    30001 tctcattacg ctgatacgtt tcaaattgat gcggataacg atttttttca tccatttctc
    30061 cttgactagt attgatcagt gaccaatcag accagtcaac aggtggaaaa taagcatctc
    30121 catcgaatac atgatggatg actgtacggt ataatatcgt acaaaatggc agaaattctt
    30181 gataaacagc tgaccctcca gcgatgaaaa agataccgtc tgtttctttt tccagagcaa
    30241 gcacgtcttc cacactatgc atgacatgca cgccttctgc tttataggaa gaatctctcg
    30301 tcaaaacaat cgtctgacga ttcggcaacg gacggctacc catcccttcg aatgtttttc
    30361 gtcccatcac tagtgtattt gcttctgtca tttgtttgaa gaatttcaga tcatttggca
    30421 aacgccaagg aagacggtct tcttttccaa tcaatccgtt ttcatcctgt gcccaaattg
    30481 cagctaacat tttctcatcc tttctgattc taaacagcga taggagcttt gatcgccgga
    30541 tgtgggtcat atccttctaa ttggatatct tccatctcaa aatcaaatac tgactgcttt
    30601 tcctgattca aaactaaagt cggaaatgcg cgtatttctc ggctcaattg ttctttcatt
    30661 tgttcgatat gattgatata aagatgagca tctcctaatg tgtgaacaaa ttcgccaacc
    30721 gctaaacctg tttcatgtgc aattaggtga gtcagcaacg cataacttgc aatgttgaac
    30781 ggaacgccta gaaacacatc cccactgcgt tgatacaatt ggcagcttag ccgtccctct
    30841 tgaacataaa attgaaacat cgtatgacaa ggaggcaaag ccatgctagg aacatcttcc
    30901 ggattccatg cagaaacgat caatcttcta gaatcaggag tctttttgat tgcttcgatc
    30961 acttgtttga tttgatcgat aaagccaccg tcttttgttt cccagtgacg ccattgatag
    31021 ccgtagatgt gtccaagatc gccgtatgtt tcagcaaagg aatcatcggt taggatcttc
    31081 tcgcaaaaaa gcttgctctg ctttttatat tcttcattga attcaggatc tgtcaaacat
    31141 ctatggccaa aatcagtcat atctggtcca ttatagtctg gacttttgat gtagcgttca
    31201 aatgcccatt catcccaaat atggttttta ttttgtaata gatagcggat attcgtatct
    31261 ccttttaaaa accaaagcaa ttcgcttttg atcaatccaa aaggtactcg tttcgttgtt
    31321 aacaaaggaa accctttttg cagatcaaac cgcatttggt atccgaataa gcttaatgtg
    31381 cctgtgcctg ttctgtcttc tttacgatgc ccttcttcca acaattttct tcctaagtca
    31441 agatacgctt gttccatcct ttttcctcct aatcttctac ccattcactc aagtattccc
    31501 accgtgccat tttttcttca agttgtgctt ctgtttcaga tacgtcgttt tgcaattctt
    31561 gtaatcgagt gaaattatct ccttgatgat tcatctcttc ttggagcaat gagatttttt
    31621 cttcaagttc tgcaatctca tcttcaatcg tttcccattc tttttgttcc atgtaagata
    31681 atttcttctt ttcagcaggt tccttgctct gtatttttgg tttcgctttt tcagcaggct
    31741 gctgggtctc tttttgcttc agtaaataac tggacatgtt tccgtaataa agttcttgtt
    31801 ttccgtttcc ttggaatact aataacttat ctgctacctt gtccaagaaa taacggtcat
    31861 gggaaacagt gatcacagca cctggaaaac ttctaaaata atcttccaaa atcgtcaatg
    31921 ttgctatgtc caaatcattg gtcggttcat ccaataataa aacattaggt tgctgaatca
    31981 gcagtttcaa caaaaataac cggcgttttt ctccgcctga aagtttgcct atcagtgtcc
    32041 catgcataaa tcttggaaat aaaaatcgtt caagtaattc tgctactccg atttgactgc
    32101 catctgctgt ttttgcttct tcagcagctt cttgtaagta agcaatcatc cgtttatctg
    32161 gtgccatttc ttcattttct tgtgtataat acgcaagatg aaccgtttca ccgatagaca
    32221 ttgtaccgct atccagggga attctgccag ctagaatatt aagcaatgtc gattttcccg
    32281 caccattttt accagtaata cctagtcttt cccgagattg aatcaataaa tctaaatgtt
    32341 ctagcagtgt ttggtcattt atacggtaac tgccatcttt gatttccagt acttttttcc
    32401 ctaaacgttg tgtcgctagg ttcagctcta aatcatcttc ttggtttact tggtacaagt
    32461 tctcctttag atcttcgaaa cggttgatcc gtgcttgctg cttggtgcca cgtgcctgta
    32521 cgccagccct catccaagct aattcctgtt tataaagccg cttgcgcttt tcttcttgtt
    32581 ctacttctac acgatcacgt tctgcttttt ctaagacgta agcttcataa tttcctttat
    32641 actcgtataa ttttccaaat gaaagttcga agattcggtt tgctactcta tctagaaaat
    32701 aacgatcgtg ggtaaccatc aatagtgcgc cacggtattg tttcaaatag ttttccaacc
    32761 actcgatcgc ttcataatct aaatggtttg ttggttcgtc tagtaatagt aaatcgggtt
    32821 catcaatcaa cacttgcgcc agactaactc gttttttctg accacctgat aaagtactga
    32881 tttttttatc taattcactg attcctaatt tttgcaaaat aatctttgcg ttggtatctg
    32941 tagtccaagc atcttcttta ttcatttgtt cttctgcttt agcataacgt ttttgcacat
    33001 cagggtcttc tccgttttcc cctagttcga tcaatgcttc ctcatatgct cgaacagttt
    33061 ggatcaaggg gctgtttcct tgaaagactg cttggagaac agttaattca tcggaaaatt
    33121 cagctgcttg agaaagataa ccaatacggt aatcagttgg ataaaacact gtttgccgat
    33181 caccgtcgct gctatcgata ccggcaataa tatttaagag acttgtttta cccgtcccgt
    33241 tcgtcccaat caacccgata cggtcttttt cgtggaccaa aaaagaaatc tggtcaaaca
    33301 aatctttttc accataagtt tttttgaggt cagttacttt taattctttc aacatgttcc
    33361 ctccttatct tggatattgt atcataaaaa tagtgaactt caggaaaatt tttttggtat
    33421 ttgttctttt tgtgttctca aaaaaagaac aggaccgatt cgatcctgtt ctgatatgtt
    33481 ttgtttttta taaaacttga aaatttttac
//
LOCUS       DAIGOK010000014        33288 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.014, whole genome shotgun sequence.
ACCESSION   DAIGOK010000014 DAIGOK010000000
VERSION     DAIGOK010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33288
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.014"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            134..409
                     /locus_tag="NOA01_000508"
     CDS             134..409
                     /locus_tag="NOA01_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HCI0631734.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            415..1182
                     /locus_tag="NOA01_000509"
     CDS             415..1182
                     /locus_tag="NOA01_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HCI0631735.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            1350..3179
                     /gene="typA"
                     /locus_tag="NOA01_000510"
     CDS             1350..3179
                     /gene="typA"
                     /locus_tag="NOA01_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HCI0631736.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            3440..5212
                     /gene="recQ"
                     /locus_tag="NOA01_000511"
     CDS             3440..5212
                     /gene="recQ"
                     /locus_tag="NOA01_000511"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HCI0631737.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            5326..5628
                     /locus_tag="NOA01_000512"
     CDS             5326..5628
                     /locus_tag="NOA01_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HCI0631738.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            5631..6794
                     /locus_tag="NOA01_000513"
     CDS             5631..6794
                     /locus_tag="NOA01_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCI0631739.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            6820..10248
                     /locus_tag="NOA01_000514"
     CDS             6820..10248
                     /locus_tag="NOA01_000514"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HCI0631740.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            10269..11393
                     /locus_tag="NOA01_000515"
     CDS             10269..11393
                     /locus_tag="NOA01_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631741.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            11383..11820
                     /locus_tag="NOA01_000516"
     CDS             11383..11820
                     /locus_tag="NOA01_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCI0631742.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            11804..12142
                     /locus_tag="NOA01_000517"
     CDS             11804..12142
                     /locus_tag="NOA01_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HCI0631743.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            12261..12821
                     /gene="rsmD"
                     /locus_tag="NOA01_000518"
     CDS             12261..12821
                     /gene="rsmD"
                     /locus_tag="NOA01_000518"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HCI0631744.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            12818..13309
                     /gene="coaD"
                     /locus_tag="NOA01_000519"
     CDS             12818..13309
                     /gene="coaD"
                     /locus_tag="NOA01_000519"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCI0631745.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            13299..14342
                     /locus_tag="NOA01_000520"
     CDS             13299..14342
                     /locus_tag="NOA01_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HCI0631746.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            14501..15229
                     /locus_tag="NOA01_000521"
     CDS             14501..15229
                     /locus_tag="NOA01_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCI0631747.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            15345..15590
                     /locus_tag="NOA01_000522"
     CDS             15345..15590
                     /locus_tag="NOA01_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCI0631748.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            15587..15805
                     /locus_tag="NOA01_000523"
     CDS             15587..15805
                     /locus_tag="NOA01_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCI0631749.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            15941..16480
                     /locus_tag="NOA01_000524"
     CDS             15941..16480
                     /locus_tag="NOA01_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HCI0631750.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            16473..18746
                     /locus_tag="NOA01_000525"
     CDS             16473..18746
                     /locus_tag="NOA01_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HCI0631751.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            18784..19830
                     /gene="holA"
                     /locus_tag="NOA01_000526"
     CDS             18784..19830
                     /gene="holA"
                     /locus_tag="NOA01_000526"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCI0631752.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(19919..20173)
                     /locus_tag="NOA01_000527"
     CDS             complement(19919..20173)
                     /locus_tag="NOA01_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCI0631753.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(20573..20842)
                     /locus_tag="NOA01_000528"
     CDS             complement(20573..20842)
                     /locus_tag="NOA01_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631754.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(21312..21767)
                     /locus_tag="NOA01_000529"
     CDS             complement(21312..21767)
                     /locus_tag="NOA01_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCI0631755.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(21905..23833)
                     /locus_tag="NOA01_000530"
     CDS             complement(21905..23833)
                     /locus_tag="NOA01_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HCI0631756.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(24030..24863)
                     /locus_tag="NOA01_000531"
     CDS             complement(24030..24863)
                     /locus_tag="NOA01_000531"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCI0631757.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(25055..25759)
                     /locus_tag="NOA01_000532"
     CDS             complement(25055..25759)
                     /locus_tag="NOA01_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCI0631758.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(25782..26408)
                     /locus_tag="NOA01_000533"
     CDS             complement(25782..26408)
                     /locus_tag="NOA01_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCI0631759.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            26734..29109
                     /locus_tag="NOA01_000534"
     CDS             26734..29109
                     /locus_tag="NOA01_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCI0631760.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(29133..29624)
                     /locus_tag="NOA01_000535"
     CDS             complement(29133..29624)
                     /locus_tag="NOA01_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HCI0631761.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            29811..30731
                     /locus_tag="NOA01_000536"
     CDS             29811..30731
                     /locus_tag="NOA01_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCI0631762.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            30885..31229
                     /locus_tag="NOA01_000537"
     CDS             30885..31229
                     /locus_tag="NOA01_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HCI0631763.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(31419..31919)
                     /locus_tag="NOA01_000538"
     CDS             complement(31419..31919)
                     /locus_tag="NOA01_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCI0631764.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            32447..32530
                     /locus_tag="NOA01_000539"
     tRNA            32447..32530
                     /locus_tag="NOA01_000539"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32481..32483,aa:Leu,seq:cag)
     gene            complement(32854..>33288)
                     /locus_tag="NOA01_000540"
     CDS             complement(32854..>33288)
                     /locus_tag="NOA01_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HCI0631765.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT        11010 a         5771 c         6783 g         9724 t
ORIGIN      
        1 aaaaatcagt tgcgttttgt tttttagaat tatcaataat attattatgt aaacttaaaa
       61 atatgacaac ttcttggttt attaccactt cctatgctac actgaagagg aatatgaaat
      121 aggagagttg aagatgcaag aatatcagta tccacttgat ttagattgga caacagacga
      181 aatggtcatt gtaatgaaga tgtgggaaga tctggaacgt gcgaatgaaa aagggataaa
      241 caatgaagct tttttgaaga cttatcagca gtttaaaact gttgtcaaat ccattggtga
      301 ggaaaaacga ttgggacggg aatttgaaaa agcttccggt tattctttat atcgtacagt
      361 gcagcaagca aaaaaaaatc caaacaaaac tttgagaatg aacgggtgag ataaatggaa
      421 cagatgattg atcatatcaa gtcctggctt gaagaagctg gtgagaaaat caaatcttct
      481 caacaaacat taacaatctc gcaaaaatca aatcgaacgg atctagtaac aaatgtcgat
      541 cgaaacatcc agaattttct aatcgaaaaa atccattcct ttgatccaga ggcgaaaata
      601 ttggcagaag aagaagggtg taatcaatta tcggatctcg atggaagagt ctttatcatc
      661 gatcctattg atggcacctt gaattttgtg attgaagggg aaaatttttg tattatgctc
      721 gctgtttatg aatccggcgt tggaaagctt ggtttcattt atgacgtgat gagaaatgaa
      781 cttttttggg gtggaaaagg aataggcgtg tataaaaatg atgagccatt gcctagacca
      841 gcaaataaat ccttatcgga aggcttactt ggtgtgaata gttatctttt cggtcataat
      901 aaatatggaa tccgagaaat tgcggaaagc agcatgggga cacgtatgta cggatgcgct
      961 ggtcttgaac tagtggcaat cttgaaagga aaccacattg cttatatttc aaaccttagc
     1021 ccttgggatt atgcagcggg aaatgtctta ttggaagagt ttggtatggt aacaagcggt
     1081 ctttttggta ctcctttgac atttgaaaaa agggaatact atttagcagg gacttcttgt
     1141 gcatatgcag aaatcgcaga ccttttgaaa aaagaaggct gaaaaaaagg tagaaatcat
     1201 tcacaaaaat gataaaaacg agcaaaagat taattttctc gaaaaaatga aaacatttta
     1261 ctatatttca tgaaaaaagt ctgctataat tagcagtcga gtaaaatttg ccctgtcata
     1321 aagaaccaat aaaaaaggag ttagttacat tgaattatag aaacgatatc cgtaacgtcg
     1381 caattattgc ccacgttgac cacggaaaaa caacattagt tgatgaacta ttaaaacaat
     1441 cagatacatt agacgcacat acgcaattgc aagaacgtgc gatggactca aatgcgctag
     1501 aaaaagaacg tggtatcacg attttagcca aaaacacagc tgtagactat aaaggtattc
     1561 gcgtaaacat catggatact ccaggacacg cggacttcgg tggcgaagta gaacggatca
     1621 tgaaaatggt tgacggcgtt gtgttggtcg tagatgcgta tgaaggaaca atgcctcaaa
     1681 cacgttttgt attgaaaaaa gcactggaac aacatatcac accaatcgtt gtcgtaaaca
     1741 agattgacaa accttctgct cgtcctgagc atgtcgtaga tgaagttctt gaactattca
     1801 tcgaattagg agcagatgat gatcagttag actttcctgt gatttatgca tctgcactaa
     1861 atggtacgtc aagcttgtca gatgatccag cagatcaaga accaaccatg gcacctattt
     1921 ttgatacaat tattgaaaaa atccctgctc ctgtcgataa cagcgacgaa ccattacaat
     1981 tccaagtttc attacttgac tacaacgatt atgtaggtcg tatcggaatc ggacgtgtat
     2041 ttagaggaac aatcaaagta ggagaccaag tagcattaat taaattagac ggaacagtta
     2101 agaaattccg tgtaacgaaa ttatttggtt tctttggact aaaacgtcta gagattcaag
     2161 aagcaaaagc tggagacttg attgctgtct caggtatgga agatatcttt gtcggtgaaa
     2221 cagttactcc tgtggatcat caagatgcgt tacctatcct tcacattgac gaaccaacat
     2281 tgcaaatgac tttcttagtt aataattcac catttgccgg tcgagaaggt aaattcgtaa
     2341 ccgctcgtaa gattgaagaa cgtttgatgg cagaacttca aacagatgta tccttacgag
     2401 ttgaaccaac aaactctcca gatgcatgga cagtttctgg tcgtggagaa ttgcatttgt
     2461 ctatcttgat tgaaaacatg cgccgtgagg gatacgaatt gcaagtatct cgtccagaag
     2521 ttattgaaaa agaaatcgac ggtgtaaaat gcgaaccgtt tgaacgcgtg caaatcgata
     2581 caccagaaga atatatgggt agtgttatcg agtccttgag cttacgtaaa ggtgaaatgc
     2641 aagatatggt ccatacagga aacggacaaa tccgtttgac attcttaaca cctgcccgcg
     2701 gtttgattgg ttattcaact gaattccttt caatgacacg tggttacgga atcatgaacc
     2761 atacttttga ccaatatctg ccaatgcttc ctggtcaaat tggcggacgt caccaagggg
     2821 cgcttgtttc gatcgataca ggaaaagcaa cgacttattc tatcatgagt atcgaagaac
     2881 gcggtacagt cttcgtagaa ccaggtacag aagtatatga aggaatgatt atcggggaaa
     2941 acagccgtga taatgatttg actgtcaaca tcacgaaagc aaaacaaatg acaaacgtac
     3001 ggtctgctac taaagaccaa acatctgtca tcaaaaaacc aaaacaattg acgttggaag
     3061 aatctttgga attcttgaat gatgatgaat attgcgaagt aacaccagaa agcattcgct
     3121 tgagaaaaca aattttagag aaaaatgctc gtgaaaaagc atctaaaaag aaaaaataat
     3181 ctgtaaaagg atgaagagag aaaccagctt gttataaaaa atagctggtt tctttttttg
     3241 tccaactctt ttctgtggag aagtcatctt tgtcaatgga cataaattca gaaaatacgc
     3301 cgaaagacgt ttaaaagact tttaaaaaac ttatttctag agagtttttg tttcataata
     3361 agataatgtt tcacgaaagg agatcaactt gtccttcgaa gaaaatcgaa gtaaaatggc
     3421 aagagaaagg agcgggaaga tgaacgactt attaaaacaa tactttggct atgatgagtt
     3481 tagacctggt caaaaagaaa ttatccagaa agtcattgat caggaaaatg tattaggaat
     3541 catgcctacc ggaagtggga aatcaatctg ttaccagctt ccagctttac tattagatgg
     3601 gcttactgtg gttgtgtccc ctttaatctc tttgatgaag gatcaggtgg acgcggcgaa
     3661 tcaattaggt atacccgcca cttttatcaa tagctcgtta gatggttacg aaacagctag
     3721 acgcttccaa gaaatcgata gacagcagta ccgtttactt tatatcgctc ctgaacgttt
     3781 tatcatgccg gattttatcc aagcaatgaa gcgatggaac gtccgtatga tcgctattga
     3841 cgaggcacat tgtatctctc agtgggggca tgatttccgc ccaagttatt tacagatggc
     3901 gaaccagcta gatcaactgc caaatcgtcc tgtaattgtt gctttgacag ctacagcgac
     3961 tgtgcaagta gctgctgaca taaaaagact tttaaaaatt ccagaaaaca accatattca
     4021 gactggtttt gaacgagaaa atctacgatt tcaagtaatt aaagatcaaa aaaaagaaca
     4081 atatctaatc gaatatttaa agatcaacaa aaatcaatct ggtatcattt atgcagcaac
     4141 gagaaaagaa gtagaccgtc tctatcattt gcttaaaaaa ttcgattttt ccgtgggtag
     4201 atatcatggt ggtctaaatg agaatgagcg aacagaaatg caagaagcct ttttatacga
     4261 tcgcttgcag ctgattgtag cgacaaatgc atttggtatg ggtataaata aaagcaatgt
     4321 ccgttttgtt atccattatc aaattcctgg ctctctagaa gcatactatc aggaagctgg
     4381 tcgggcggga agagatggtc tttccagtga agccatcctg cttttctctc ctcaagatat
     4441 ccaagtccaa aagtttttta tccagcagtc ccaaagagaa gaagggcaaa agcaaaaaga
     4501 atatgaaaaa ttaaaagcga tgacagaata cgtgtatatt gagtcctgtc ttcagcaata
     4561 catcctaaac tattttggcg agacaagcag tccgtgcaac cgttgtggta actgtttaga
     4621 tgacagagaa atagtagaag taacgacaga agcacagatg gttctttctt gcttaaaacg
     4681 tatgggtgag aactatggta aacaaatgct gatgaaagtc ttagcaggat cgaaagaaca
     4741 aaagctgcaa gctttaggtt ttggacattt atctacctat ggactgatga aaaaccaatc
     4801 gcaaaaagaa acgatgcagt taattgaata tttgatatcg aatggctatc ttctgaccat
     4861 caatggtgag tatcctgttt tgaaagtaac agaaagaggg atacaagtat tgaaaggaca
     4921 agaatctgtt tatcgaaagg aacccaagaa agtccagcag ctatcggatg aagaaaccga
     4981 tacactattt gaagtgctgc gggaactgcg aacggatcta gcatcagaag caggcgttcc
     5041 cccgtatgtc gtattttcag attccacatt gaaagaaatg agccgaatac gtccatcaag
     5101 cagattggag atgcttcaga taaaaggagt tggccaaagc aagcttgata aatatggcga
     5161 agccttttta tctcggataa aaaatgcgga ctctaacgtt ctggataagt agaaagaacg
     5221 aatacgtaaa aatgaataaa aacaactggt taaatctgtt ttttttgctc cttatgaaaa
     5281 tcaaatatgt tataattaag gaattagaat gggaggtaag cacagatgga ttctttgtcg
     5341 aaaaaacttg cgatccaagt attaaatgat gaagcagaac ggatcaaacg attaatccga
     5401 aatcaaaaca acagtctctg tatctcacaa tgcaaagcct tcgaagaagt ggtagataca
     5461 cagatgtacg gcttttcccg gcaagtcggt tttgctgtcc gtataggagt aatcggaaag
     5521 aacgagggcc agctgatgtt aagtgagcta gaacgagaat tgaatcagtt atatagtgag
     5581 gtttaccaag aaatttatga taaaaaagaa gttggcaagg aggaatagac ttgcctaaga
     5641 aagtgaaaaa aaggcatcta ttagactata gcatcttgat tccctattta attttatgtg
     5701 tattgggatt gatcatggta tatagttcta cgtcgtatct attattagaa aacggacaaa
     5761 atccgtccgc atcagtcatt aaccagagta ttttttgggt gcttagcttg attgctatcg
     5821 cactacttta taaaatgaaa acagatgttt tgaaaaacca gcgtttgatc atggcagcca
     5881 tcgcagtttt gacgattttg ctgctgattg ttgtgttttt tggtaaggag atcaatgggg
     5941 cgaaaggttg gctgcaaatt gcaggattct cgatccaacc tgccgaatac ctaaaaatca
     6001 tttctatctg gtacctgtct ctgactttat ctaaaagaca aaacagtgta caaaaagatt
     6061 ttcttgggac tgtgaaacgt ccacttgcaa tggttatcgg acttactgca ttagtcgcta
     6121 ttcttcccga ttttggtaat gcggcagtta tctttttgat tattttggtt ctgcttttag
     6181 ccagcggagt caattatgtg tatactttga ttgtaggagt aggaggcttt tgtctaagta
     6241 cttttacgat ctggctgatc aatatcacaa acggaaagat cttacccgga cgtttacagt
     6301 atatctataa tcgatttgct atttaccaaa atcctttctc agacgaatta aataaagggc
     6361 atcaactagt caacggctac tatgccatgt tcaacggagg actatttggc aggggattag
     6421 gtaacagtat ccagaaaaaa ggatttttac aagaagcaca aaccgatttt atttatgcaa
     6481 tcgtagtgga agaactggga gtgattatgg ggattttgat attagcgttg ctattcttta
     6541 tgattgtgcg cattgtcctt gtaggtatcc gttcaaaaga tccgttcaat tcgcttttgt
     6601 gcattgggat cggcgcaatg tttttgatcc aagtatttgt caatcttgga ggaattactg
     6661 gcgttattcc tttgacaggg atcacgttcc catttttaag tcaaggaggc tccagtttgc
     6721 tgatgctttc gatttgtgtc ggctttgtcc tcaacatcag tgcggatgaa aaacgcaaga
     6781 gcttaggttt ataataaaaa aagatgtagg agtggttcga tgaaaaaagt tttagttgct
     6841 aacaggggtg aaatcgctgt acgtgtattt cgtgcttgta cagaattagg aattaaaact
     6901 gttggaatct atgcagaaga agatgaatat tccgtacacc gtttcaaagc ggatgaagca
     6961 tatttagtag gacaaggaaa aaaaccgatt gatgcgtatc tagatattga agggatcatt
     7021 tctatcgcga aagaatgcgg agctgacgct attcatccgg gttacggtct tttgtcggaa
     7081 aacttgaatt ttgcgcaacg gtgcgaagaa gaaggcatca tttttgttgg acccaaactg
     7141 catcatttag atatttttgg agataaaatc aaagcaaaag ctgcagctat cgaagcgggg
     7201 attgcttcga ttccaggcac agatggacca atcgcttcta tagacgatgc cttggagttt
     7261 gcgaaacaat acggttatcc gattatgatc aaggcagctc taggcggcgg cggacgcggc
     7321 atgcgtgtag ctcacgatga aaagagtgca agagagggct acgaacgtgc gaaaagcgaa
     7381 gcaaaagcag cctttggtag tgatgaagtg tatgttgaaa aatacatcgc taatcctaag
     7441 catatcgaag tacagattct cggggatacg catggaaacg tcatccatct ttttgaaaga
     7501 gattgttctg ttcagcgccg tcaccaaaaa gtagtagaag tagcgccttg tgtctctatg
     7561 aatgaacagc aacgtcaaaa aatctgccag gcagctgtcc aattgatgaa atatgtcggg
     7621 tatgtcaacg caggtacagt agaatttctt gttgaaggag atgattttta ctttatcgaa
     7681 gtcaaccctc gcgtccaagt ggaacatacg atcacagaaa tgattacaga tattgatatc
     7741 gtaacaacgc aactgttgat tgctcaaggt ttggatctgc ataaagaaat cggattgcct
     7801 cagcaagaag gaattaaatt aaatggttca gccattcaat gccgaatcac tacagaagac
     7861 ccgttgaata actttttacc agacacaggg aaaatcgata cgtatcgttc acctggtggt
     7921 tttggcgtac gtctggatgt cggaaatgct tacgcgggct atgtagtgac accgtatttt
     7981 gattcattgc tagtaaaagt atgtacgcat ggtgctacct ttgaaacagc cattcagaaa
     8041 atggaacgtt gcttgaaaga atttagaatc cgtggagtga aaacaaacat tccatttatg
     8101 ctcaatgtca tcacacatcc tgaattccaa tcaggaaatg caaaaacaac cttcattgat
     8161 agtacagcaa cattatttga atttcctcga ctacgagacc gcggaaacaa aacgatgaaa
     8221 tatatcggtg aaattactgt gaatggtttt ccaggtatcg aaagtggcga aaaaccattt
     8281 tatgaagaac cacgtatgcc taaagatcta atcacaagaa ctgattacgt aacggctaag
     8341 aatgtattag atgcgaaggg tgccgatgct ttggtcgaat ggatcaaagg acaggaaaat
     8401 ttgttgttga cggatacgac atttagagac gcccatcaaa gtctgctagc aactcgtgtt
     8461 cggacaaagg atttcaagca gattgcacgt ttgactggag aaggtcttcc tgaactcttt
     8521 tcaagtgaga tgtggggggg agcaacattt gatgtggctt accgcttttt gaatgaagat
     8581 ccttggcaac ggttgagaaa aattcgttct ttaatgccta atacgttgct gcaaatgctt
     8641 ttcagaggtt caaatgccgt tgggtattct aattatcctg acaatgtatt ggtggaattt
     8701 gtcaaagaag cagcagcgca aggaatcgat gtatttcgaa tctttgatag tttgaactgg
     8761 actccacaaa tggaaaaaag tatccaagct gtacgggata ctggtaaaat cgcagaggca
     8821 gctatttgct atactggcga tatcaacgat ccaagtcggg caaaatacaa tgttcagtat
     8881 tataaagaca tggcaaagga actagaacaa ctgggtgcgc atatcattgc aatcaaagat
     8941 atggctgggc tgcttaaacc gcaggcagct tatcgtttga ttagtgaatt gaaagaaacg
     9001 acagatctgc caatccatct gcatactcat gataccagcg ggaatggaat catcacttat
     9061 tctgctgctt caaaagcagg agtagacatc gttgacgtgg caatgagtgc aatgagtggg
     9121 aatactagtc agcctagcat gagcagcttg tattatgcac tggtcaatgg tccacggctg
     9181 ccagaaatca cgattgaaaa tgcacagaaa ttaaaccatt attgggaaga tgtgcgcatg
     9241 tattacaaac cgtttgaaaa cggcttgaat gctcctgaaa cagaagtata tatgcatgaa
     9301 atgccaggag gacagtattc gaatctacag caacaggcaa aagctgtcgg attaggtcat
     9361 cgctgggatg aaatcaaaca aatgtatcat actgtcaact tgatgtttgg cgatatcgtt
     9421 aaagtaactc catcatctaa agtagttgga gatatggcac tatttatggt ccaaaatgat
     9481 cttacagaag aagatattta tgaaaaaggc gaaacactaa gtttccctga atctgttgtg
     9541 actttcttcc aaggagaact agggcaaccc gttggcggat ttcctaaaaa attgcaaaag
     9601 attattctta aaggcagacc tgcattgaat gaacgtccag gtcttttggc tgaatcagtc
     9661 gacttcaacg aagtaaaaaa agaattggca gaaaaaattg gctatgaacc aaaacaagac
     9721 gaagtattaa gctatttgat gtatccgcaa gtctttttgg actatcaaaa agcatataat
     9781 caatttggcg atgttacatt actggacaca ccaacatttt tccaaggtat acgtttgggt
     9841 gaaacaatca atgtacaaat cgaacgcgga aaaacgctta tcattcgttt agatgaaatc
     9901 ggagaacccg atatcgaagg gaatcgcgta ctgttcttca atttgaatgg acaaagacgt
     9961 gaaatcacta tcaaagataa ttcgatcatc agtacggtcc aaacccgatt gaaagctgaa
    10021 ccgacaaacc gagaacaaat tggagcaacg atgtctggtt ctgttttgga tgtattagtg
    10081 aaaaaaggcg ataacgttaa aaaaggtgat acgttgatga ttactgaagc gatgaaaatg
    10141 gaaacggcca tcgaagcacg ttttgacgga gaagtcgcac atgtatatgt gtcttcagga
    10201 gacactattt cgtctggtga ccttttgatt gaagtgactg agaaataata agcgaaaggt
    10261 ggggaaacat gaaaagactg ggtttgtttt ggctgattct gctattagtt attgtggcat
    10321 tctacatgga accagtcttg tttccgccaa aacttgaagt gaaggaaaac tctgatcaac
    10381 aaatatcaaa ccaaatgaca acgtggaaat accaggagct taaagcaact ggtttttcta
    10441 cttatatcgg aaaaccgatc gatcagctgg aaaaggattt tggccaagcc tatgatcgat
    10501 taactagcgg atttggtttt gaaacaagat attatgaaaa taaagctagt catctcacat
    10561 ttgaagcgaa catccaagac agcagagtca caactgttaa agtactgcaa acagggcctg
    10621 ctgatatcgc gccgttttat ttagggatga cgatgcaaga tcttacgaaa atcactacta
    10681 tctatactaa tttcactttt gagtaccatg atacagaagt tggaatcgaa ctaatggaag
    10741 aggatatgaa ttatcgtccg ctaattgctt ttgataacga cacttttgct attttatttt
    10801 ttgatcaaac aacaaacggc ctattttcag ttgtgtattt agataaagat agtcttttga
    10861 aactattgcc atatcaagtg tttggtgaag gccttccaca ttatcaagca gataaatcgg
    10921 ctgattggga atcaatcaat cgcgttaaag aacgaaaaag tacgacttta ttaaatatgc
    10981 ttagaagaga agacgaactt cccatataca atcaaacact tgcctttcaa aaccacagtc
    11041 agctgctact acatgatttt ttgacaaggc ctgaggaaat attgtcagag gaacgtatgg
    11101 ctgattggca aaaatcgcta ggcagtgctc aaacgacagt gaaattctca ctgacgaatg
    11161 atgaattgga gaagttaatc agtaaacaaa aattagaaaa tactagtggg ctatttattc
    11221 atccggcgat tgacgctact ttttcgtttt tatattggta cagcgatcct tacaaccatg
    11281 accgtttcat ggacccggga acggattctg taggcattgc tttttctaaa gaaaatatgt
    11341 tagtcttatt gcaagaagaa aacggagaaa gtagtgatag cagtgatcgt taacgaagag
    11401 ctgtttcaac tggaggatca atgcctccaa gtcgctgaaa tgataaaaaa gagtaaaacg
    11461 atggcttctt atcttgaaca taaacagtca atggaagaat ctgcaaaagt ttgccagttg
    11521 aaagaagcgt tcttgtctaa gaaagaagca tttgaaaatg tagcggcata tggtgagtat
    11581 gcacctgatt atcgtgaaaa aaaacgagct gttcgaaaag caaaaagaca gcttgatatg
    11641 gatgagcatg ttgcagcttt ccgatatagt gaaacacagc tgcaaagtct gttggatcaa
    11701 attgtccaag tacttgccga agaagtttct aatgaaatca aagtagatgc aggtagcccg
    11761 ttttttgaaa cgaaaaaaca ctttggatgc ggaggtaatt gtcatgcaat tagacaatag
    11821 tgaatttgaa ataacaaaac gacgcgggct gatcgtatgg gtctacagct taaaacaatt
    11881 aaagaatctg aaaaagttcg gcttgttgca ttatgtctca agaagaatga aatatgtcgt
    11941 gctttactta aatgaagaga cattggaaga aacagaagaa cgaatccgta aattacattt
    12001 tgttcgaaac gtagaacgtt cttaccgacc ggatatcgaa atgaattttg ccgaaaaaat
    12061 aggaaacaaa acagtattaa aagacgaagg tttcgaaatt gaagagctaa atacagaaat
    12121 ccgattagct gaacatcttt aaaaatctct tatgagcagt ttttaaataa aaaataactt
    12181 ggataacgat cgatttttga tcattgtcca agtatttttt tggtatacta gtaccagtac
    12241 catttctaag gagtgagtag atgcggataa tatcaggaaa ttatggcggg cgccgtttaa
    12301 aaagtttagc aggggcgaac accagaccaa ctacagataa agtaaaagaa tccatattca
    12361 atatgattgg accatatttt gatggagaga cagtattgga tctgtttgca ggaagcggtg
    12421 gtttagctat cgaagcagta tctagaggct gcagtcatgc ggtatgcgta gacaaaaatt
    12481 accaagcgct aaaaatcatc aaagaaaaca tcgaaattac gaaagaacca gagaaattta
    12541 ccacaataaa acttgatgcg gacaaagcaa tcgaacggct agcagcggag aaacaacaat
    12601 tcgactactt gtttcttgat ccgccatatg caaaacaaaa aataatcgat caaatccata
    12661 aaatggaaga actgggactt ttttctaaaa ccgcagttat cgtatgcgaa acggataaaa
    12721 cagtcgaatt gccagaaact gtggctggat tccatcaaat aagaaaacag atttatggca
    12781 taacggccgt aactatctat agaaaagagg gaaatcaatg aaacgtgcat tattcccagg
    12841 aagttttgat ccttttacaa aaggtcactt agatacggtg gaacgtgcgg ctaaattatt
    12901 cgatgaggta gtaattggtg tttttatcaa tacttcaaag aaaagtttgt ttcccccaga
    12961 agaaagaatg acattgatca caaaagctgt ttctcatctt cctaatgtaa aagtcatgca
    13021 tcaagaaaat caattgacgg tagaaacagc aaaagagatt ggtgcagatg cgttgatcag
    13081 aggcatacga agtatcaaag actttgaata tgaacgagag attgctcaga tgaatcatca
    13141 tctatatgat gaattggaaa ctgtcttctt attagcaaaa ccagaatata gtcacgtgag
    13201 ttccagtatt ttaaaagaag tactgcactt tggcggagat gtgagcagct acttaccgcc
    13261 agtgatcaac gaagctttag ctggaaagcg tgaatcaaat gaaagctaaa caatggttga
    13321 aaacagtact gattatcata tttggaatgt tgatcattgg atttgtagta gttcccttgc
    13381 cgtattatgt agaatcaccg ggagccacga tcgatctgaa agaactaatt acggtcaatg
    13441 ataaggaaga taaaggttct gggtctttct ctcttacctc tgttggtatc aggcaagcta
    13501 caggtttctt agctttaaaa gctaaaatga ccccttttga agaattgatc agtgaagaag
    13561 aattgactgg tggggcgaca aatgaagaat ataatcaaat gcaacagtat tatatggatt
    13621 cttcccaaaa tgcagcaatc gaacaagcgt taaaacttgc agacaagcct tataaaatgg
    13681 aattcaaagg cgtatatgtc atgagtgtag agaaaacatc gaatttcttt ggcaagcttt
    13741 cagttggaga tacagtaaca aaagtagacg ggaaaagctt tcaaagtaca gaagcattta
    13801 tggattatgt aaaaagccaa aaagttggac aaacaatcga aatcagttat ttgcataatg
    13861 gtgaagaaaa aacagcttct ggtgatctga tcgagttacc aacagataaa aaggcaggaa
    13921 taggtatcac gcttactgac cataccgaga tcgaatcaga tacagatgtt cgtattaatt
    13981 ctggctctat cggtgggcca tcagcgggat tgatgttcac tttagaaatc tatgaacaag
    14041 tcactggtaa aaacttgcga catggtaagc aaattgcagg aactggaacg atcaacagtg
    14101 aaggagaagt tggccgaatc ggaggaatcg ataaaaaagt ggcaagtgcg gataaagctg
    14161 gcgtagagat tttctttgca ccagatgata cgatttcaga cgatgttaag aaagaatatc
    14221 ctgaaataaa aaccaactac gaagaagcaa aagctgccgc taaaaaattg aattcctcaa
    14281 tgaaaatcgt accagtaaaa acagtacaag aagcgcttga ctatctagag aaaatgaaat
    14341 gaaactaaat aattaaagag gggttgtgac acgagttctg tcacaacccc tctttttcgt
    14401 caaaaatctt cacgataaaa ataaaaacaa aagaaaaaat tgacgtaatt agatgttgaa
    14461 gggacgattt tgccagagct cactgtttct tcaattttaa atgaaaggag taatgaatga
    14521 tttttactgg tcattctttg aaaaaagaac aatgatagaa aaaatcagca agaaaatact
    14581 agcttcaccc aaattgatca tttggatgtc tatgatagtt cttatctttt gtatcgcaag
    14641 tggtttttta ttatcaaatt tgctatttta tagagaaaca gaagagactg ctgcaaaaga
    14701 aatactttct tcgtttagtt ccagtggaga agatccatca gctgaaggaa acgatgtaga
    14761 agaggatgct cgtccaaagg tcatgtacac cgatatcaaa ggaagtgtga aggagcctgg
    14821 tatctattcc ttttcttcag aagaacgggt ctatgatgta ctaaagcgtg caggtggatt
    14881 attagaagaa gcggatagcg atcgaatcaa cttttctgcg aagatagaag atcagcaagt
    14941 cctatatatc cctgctgtag gtgaagagct gccagaacat cttgatcagt tggcatcctc
    15001 ggaaggaaaa cagcctacag cagattccga gcctcccaaa ataaatatca atacagccac
    15061 tccatcagaa cttcagcaga ttcctgggat cggatcagtc aaagcgcaag aaatcattcg
    15121 atttagagaa gaaaacggat ccttccagaa agtggaagac ctgcaggaga tttctggtat
    15181 cggtgaaaag acagttgaaa aactaaaaaa cttcgttaca attaaataga atgattcaaa
    15241 atcgactggc acgagcaata aatggacgct aatggatact aagaataatc caattctaag
    15301 agcttggcag gacgaggagg tagtattttt ggataggaaa tagcttgatt gttcgcaaag
    15361 aaacattaaa aaaaccaatg ctgaatgtct atctccaaaa taaaatatct gggattcata
    15421 ttatgaatac ggctgtcagc ggcaataaca gtcaagctct acgagaacgc tttgccaaag
    15481 atgtacttag ctatacagca gataaagtat ttattctcat tgggacgaat gatctagcag
    15541 agcataaaca actttctaaa gaaacgtacc aaaaaatctg cagtggatga tagttcagct
    15601 gaaaaaagac tatagttctg atcatgcttt ctttatcacg ccaccagcag tcgatgagac
    15661 aaaacaaaaa tatcggaata acaagttgat caaagaatat atctctgttc tcacagaagt
    15721 agcaaaacgc catgattgtg aagtatcgat ttttatacgg aatttcttga acaaatggat
    15781 tcgaagaatt tttataatgg gctaatggat gacggacttc atttcggcag tgaagggtag
    15841 gatcttttgt cagacaggat gattcgttat atttaaagaa tattttgttt aagcaatgag
    15901 tactttttaa tcaacttgaa aaaacggagg aatataaaac atgttagaaa aatcttcaaa
    15961 agcatgcgat acgaataaag aaaaagttac tgataagtca cataatgaac gcattccttg
    16021 ggaccagtac tttatggctc aggcagtact gttatcttta cgtagtacgt gtacacgttt
    16081 agaagtagga gcgacactcg ttcgagaaaa aaggatcatt gctggcggct ataatggagc
    16141 agtcagtgga gatgtccact gcatagatga agggtgttac gtcgtggatg gacactgctt
    16201 gcgaacgatc catgcagaga tgaatgcact cctacagtgt gcaaaacttg gtatcccgac
    16261 ggaaggttca gaaatctatg tcacacattt tccatgtttg gcctgtacaa aggctttgtt
    16321 gcaagctggt gtgaaaggga tcaattatct ccatgactat cgaaatgatc cttatgccca
    16381 agcgttgatc gaacaattag gtgtatctgt acaccaagtt gctttagact cacagcattt
    16441 ttcaaaactt caaaatgaat taacaggaaa acatgtctag cgttttagaa aggcatgttc
    16501 gaaatcggtt gatttttccg gcaattacgt cgatgattat ctgtttgttg tattacactg
    16561 acaattgggg ttatcgtttg cttcttgccg gaattttttt atggttttcg ctaaaagaag
    16621 gacgttttat cgcgtacttg tgtattttgt gtagcataac ggtatccatt tcttataggc
    16681 tacaagagta ttcattttta gaggaaaaaa caacatctgc gcaaattact gtatttacag
    16741 atacgattcg tgcaaatggt aattttttga caatggaagg aaggttatca gagaataaac
    16801 aaaagctcca gctttcttat caagtaaaca cagaagcaga attgaaaaca tggcttaact
    16861 tccgaggaaa gggaatagaa ttgactgctg aaggcgagtt catctctcca gcaaaaaagc
    16921 ggaatcttta taattttgac caagaagctt acttcaggaa ccatagaatt tctggacggt
    16981 tccaaattca aaagattgaa cagatcacca tgcaaaatca ctggcgaaac tggcttcaga
    17041 gaaaaagagg atcttttatc tcctttgtcc aatctgaatt tccgcaaaaa acaagtgtat
    17101 atattaattc tttattatgg ggctataaag atgcaggatt cagagaacca gaagaaattt
    17161 ttagggagag tggtttgctg catctgttca gtctatcggg tctacacatc caattttatt
    17221 tagggtggct gtattattta tttcgacgta tcggatggcc actacacgtc agtattattc
    17281 cgttgagtct actaactatc tgttatgttt ctattgcagg aagttctatt tctgttttgc
    17341 gtgctagttt gctgttttta ttgcgattgc tagtcaaatt gctgaataag cattattcca
    17401 ccatggactt atttgcagtt gtcttttggc ttcttttact atttgatcct cgatttcttt
    17461 ttcatgcggg tggacagctt agttttttca tgagtttttt gtttatcctg atttcttttg
    17521 atggagattg gttgaaaaaa acggcctctc acgtcctgtt tactatgatc acgatgccgt
    17581 tgattgtatg gcatttttat gagtggccta ttttaggtag tctttttaca ttactgattg
    17641 caccggtatt caagtttctt tttcttcctg gtgtgttatt tctcacactc ttcaatcgct
    17701 ggctgccgga aactttactt ttgctgatag aagagggatt aatcttattt gagcgaataa
    17761 tcgatttttc ttcagggtcg tctctcatta taggacgttt gccccttgtg ctatctggga
    17821 tgtatatttt cggattactg atttgtatgg ataggttgaa agaacaacct gtgaaacact
    17881 tgttttatgt tggtcttttt tcttttattt ttttgacttt gccattttta cgcttttctt
    17941 caacgatcgc ttttatcgat gtcggtcaag gagacagtat ctttttgcaa agtccttttc
    18001 gaaaagaaac gattttgatc gatactggag gtcagttaca ctttgaaaga gaaaagtggg
    18061 cgcaaggtat gatacgtcct cctgctgaaa gaaatatcgt tccatatcta aaaggtcagg
    18121 ggataagtgt aatcgataaa ctcatcttga cacacgacga tacggatcat gtgggagaat
    18181 tacccacgtt atctcagcat tttactatca aaaaaattta tatcggttgg ggcgctggac
    18241 gaaatgagcc attacaaagg catttatctg aagctcaaaa aaatggtact gagatcattg
    18301 aaatcaaaca tggggacagg atcagcggtt attatgatct ttatgtattg actccttttg
    18361 aaaagggaga gggaagaaat gaagattcga ttggtctatg gatggaatat aacaataggc
    18421 gctttttatt tttaggagat ttgaatcaag caatggaaaa acaattgctt ttgatttatc
    18481 ctaatctgaa agccgatgta gtcaaattag gtcatcacgg tagccgtacg tcaagtaaca
    18541 cagattttct ttcccatatt gaagcaaaac atgggattat atcctgcggc gtaaataatc
    18601 gttatggtca cccgcacagc gaagtattgg agacactaga agaaaatcag ataaaaacat
    18661 ggcgaacgga tcaacaagga atgatcacct atcgctggca tccagttttt catcctcatg
    18721 ggctagtgga aacaatgata gactagtaat tctcatctgc tcatggtagg ataatttttg
    18781 aaagtggtga gtaatttgag tatacaagca gagttacaaa aaatacgtaa agaaaaactt
    18841 gcatcttgtt acttgatttt aggaacagaa aagtttctcc aagatcaagt tcggacagaa
    18901 attgtcaaaa aaatccaatt agagggcgaa gatgacttaa attttttaag ttttgacatg
    18961 gaaaatgcaa gtttagatga ggtagtagct gaagcagaga cgttgccgtt tttcggagat
    19021 caacggttag tattcgtaga gaatccttat tttttaacag gtgaaaaagt aaacaatgga
    19081 atcgaacaaa atactgattt attgacggct tatttgaaag aaccattaga atcgactgta
    19141 ttagtattct ttgcgccata tgaaaagttg gatgaacgga aaaaagtaac gaaacaattg
    19201 aaaaaaacag caattatcat caatgtccaa cagttgaatg aaaaagaagt acgtcaatat
    19261 ctgatgaata ccttagagaa ttcctcaatc aaaatggaac gaagtgcaat tgatttattc
    19321 ttgaggttga ccgacttaga tttatccaaa atgatgagag agttacagaa attggtcctt
    19381 tatggacaaa atcaacagca aatcacggta agagaagtag aagaattagt tcctaaaacg
    19441 ttagaacata atatttttga catgacccaa tatattttaa aagggaagac ggaacaagct
    19501 ttgcgtttat acgaagattt agttatgcaa ggggaagaaa caatcaaaat taatgcgatt
    19561 ttgctttctc aattacgttt atttttacag acaaaatttc ttgtaaagat tggttatcag
    19621 caggcaaata ttgctgaaac attgaaaatc catccatatc gtgtaaaact tgccatgcag
    19681 gaagtaaaaa aattcgatga acatttgctg gtacagttgt ttgatcaatt agttgagatg
    19741 gattatcaaa tcaaaacggg acaaatcgaa aaagaattaa gttttcaact attcgttttg
    19801 cgcacagggc agttgttaag aaatcgataa gaagaaagac gagggcaaag catgcgggaa
    19861 ggtcgtttgt ttttgtcctt tttttgacaa ataaaaaaat cggccgaagc cgattctttt
    19921 atttagcgat ttttttgctt aaacgagatt tgtcgcggtt agctttgttt ttgtggatta
    19981 gaccttttga ttcagccata tcaatagctt taacagcttc tttatataac gcatctacgt
    20041 tgtcagcacc tgaagctaca gcatcttcaa attttttgat tgcagtacgc atagcgcttg
    20101 tttgagatga atttttcaca ttagcgtttt cgcttgtacg aacacgtttg attgcagatt
    20161 caatgtttgg cattagtttc acctccgata acttaagttc ttacgcgtgt taccgcataa
    20221 ttcaacattc attattatac ctaaaggcag cgtgctttgc aatatgaaca tgacaagttt
    20281 ttttccttaa cagtctttta ttaggatttt ttttcagtaa aaacggatag tacaagctga
    20341 aaaaaatcaa aaagacgcta ttgtgggagg caatagcgtc aaaccaaact agatttttat
    20401 ttatctattt aaacaaagtt tgaatgggaa tgtttggtac taagttatta tataacaaag
    20461 agaaccgttt gcaaagtttt ttttgaaaag ggatacgatt ccctgttgtt ctttgttttt
    20521 ctattaagaa actgcagtta ttttataacc agtatccttt gatcagtaaa ggctattctg
    20581 gtgtaccgat tattttaatg tattcatcga tattttttat caacattttt tttatgacca
    20641 actggttccg acggttccat ttgccactta cttccagcac atatttattt aggggcaaac
    20701 gtaagatata ttttgccaaa tagcgatctt taagcaggcc attgatcaca cggtcctcaa
    20761 gctgcaagtt gatcccagtc agcatcattg gctgttcttg taaaacgtac gtatcaatta
    20821 aaagaccttc aaaagtttcc acaagcgtct tcctttcttt tcaaagtaac ctgaaaacag
    20881 gcactaattc ttccattttg atattcgttg aagtggatgc gatcattctt tcttttgtcc
    20941 actgtaatct agtataacaa aaaagagcag aagagccaaa aagaaaaaag agcatcgaat
    21001 tctataggaa gtctcattgg cacggcctaa attattacaa aacattgatt aaaaaaagcc
    21061 cctgcatcgc aggagcatcc cctatatcgt tttaataggt caatcaaaaa gcagtatcaa
    21121 acacattttg tatcaaatca ggaaataata gataagctaa gcccagaatc aaaagtaccg
    21181 ctagaagaaa ccagccgatt gttcctatga aatactttaa tttttcgaac attgtatcac
    21241 ctcgttgtca gtatatcacg gatcagtaaa ttgggggtgt taagaatctc ctaaaataga
    21301 caaaatttgt tctattttcc taaagcaaat acttgtaaga tacagtaagt gataccagaa
    21361 gcaatgaccg gtccggcggc gattcctctt aagaagacaa cacctaaaat cgtgccgaaa
    21421 acaagtgcaa cagtgatttg tggatcagaa gcaatcaatc ctacaccctt ggaagataag
    21481 acagccacta gcccaccgca aaaaacagct acccagccaa taggggattt aaaggcttct
    21541 aatagatcac gtagaccgat ttctcctgtt gcgataggga ttagaatagc cgctgtgatg
    21601 atcgtcaccc caatattgat tcctttgcct tgaagccaag ggaaaaagga acgggaggct
    21661 ggtatgagct tcataatcaa aacaaaagct gccgctatca tcagactttg atttttagct
    21721 aaaattgcta caagtaaaat aattcctaaa aaaagccaac tttccatgaa atatattcct
    21781 cactttcttc gaaacaatca taacatgttt ttagaaagtt acaaaagtag gaattcatcc
    21841 ttcaagacct attcataagc tgtttttttc ataaaataaa caaaatcata aaaaaacact
    21901 ttgcttattt gacagaagac agccgcgctt tgtatagtgg acaagactta catttttttg
    21961 aagaatcaga aagatcaggt aaaggtctta gtaaacgcaa gccattcaga ataaccaata
    22021 ttgtgcttcc ttcgtgtcct acaactccaa gaggtagatt taaaaattgc aggaagttag
    22081 aagcaatcaa caagagaata acaagaatcg aaaaaataat attttgtttg atgatccgcc
    22141 gcatttttaa agatagccga tgactgtaga caaatttatt taagtcgctt ttcattaaga
    22201 cgatgtctgc gatatccata gcggcatctg tcccttgccc catagcgaca cctaaagaag
    22261 cggctgctaa agcaggcgca tcattgactc catcaccaac catcgcattt acttggtaaa
    22321 gttcattctc ttttttgact agttgtgttt tttcttccgg agtacagctt gcatagtaat
    22381 gttcaatccc tacttgttca gcaatggcct ttgctgtgcc agaatggtcg ccggttatca
    22441 tacttgtatg gatgttttgg gatttaaagt aggaaattac ttgttgggta tttgcttttg
    22501 gaatatctag cagacctaaa acagcaatta actgctgatc tttagaaaga taaatcacgg
    22561 ttttgccttg attttccaaa cgttcgatca tttccgcaat ctctttgctg attatcatcg
    22621 tctcaggatc atatgcgtgc ttgccaactt tccattgcgc acctaaatag gtagtttgta
    22681 atccaaaacc agtaatttct tctattggta agtattggat ttcttcaggt attgtgcaat
    22741 cgaaatattg caaaatcgca tgagcaagtg gatgcgtcgt tttttgttcc atagcaacaa
    22801 gaagttgttg tgccaaatcc ttttcatcaa agaaaaagct gtcagttaca actggttttc
    22861 ctttagtgag cgttcctgtt ttatcaaagg caatcgcttt taattcagaa agcttttcta
    22921 aatggatacc gcctttgatt aaaatcccat tttttgcaga atttgaaaga gcagctagag
    22981 aagctggtgt agcagatgcg acaagcgcac aaggagaagc cacaactaat aataccattc
    23041 ctcgataaaa gctttcatcc caactccaac ctaggaaaaa gtgggggaga aggatcatta
    23101 gaggaacagc aagcaaaatc aattttacat aattattttc gattcgttcg ataaatgaag
    23161 ctgtttgagt tggcgtattc tgcgcttctt ctactaattg gataatctta gcaaagacag
    23221 tttcggaact cgtttttgtt actcgaatcg tgatgggatt tccttgattc agcgttcctg
    23281 catacatctc atcgcctaaa tatttttcta caggcacaga ttcaccgctt aatgcagact
    23341 catcgatcgt tgcctgtgtg ctttctaaaa agccatctat cggaacagct gctcctttag
    23401 cgacaaatac caagttatcc acctttagtt gagaaacatc tacttcttgc atttgtccat
    23461 tttcttttaa taattgggct ttttgtggtt gaagattcat caaagcagta atttcttttt
    23521 gacttttatt agttgtgtat tcctctaatg caccgcttaa acaaaagatg aatgtcaaca
    23581 ttgctccttc aaagtaatta ccgatcaaac aggcaccaat agcagctaaa gccatcaata
    23641 aatccacatt cagtgtccga tcattccaca attctttcaa gccttccgat gtttgtttat
    23701 atccaccgat gataatggca ctgatataaa aaagtggggt gatggtaaga gagaatcggt
    23761 cgaagataaa accgaagatc ataaataaaa ggcagccgat tgttgctgca aaagcttttt
    23821 tctgattagt catcgttatt agacctcctt gtcaaaacaa ggatagcata ataaaaatgt
    23881 aattgataat aataacagct aaatgagaac gataatggtt atcatttggt gattaagctt
    23941 gatgttgttt ttattattta taatgattat tattaaagaa aataaaaaac cagaacattt
    24001 ctcaaataag agaagtgttc tggttttttc tacattgcaa taaaccaata accaatcaaa
    24061 atgataccaa ggataatacg ataccagcca aaagcggtaa agtcgttttt cttcaaatag
    24121 cttagtaaga atttgatagc taagacggaa accacaaaag atacgaatgt tcctacaaga
    24181 agaacaacag tttcttccat tttgaatgaa ttcccttcag ctaaatattt gacgatcttc
    24241 aagccgcttg cgccaaacat gacaggaatc cctaagaaaa atgaaaactc tgtagcgaca
    24301 aaacgcgaag caccaatcaa gatagatcct aggatcgttg cgccagaacg ggatgttccg
    24361 ggaacaagtg ccaagacttg gaataaccca acaagaagag ctgcttggta cgtaaagtca
    24421 ttcagagaag tccaacgagg tgttttgttt ttgttgcgtt tttcgacaat aataaaggca
    24481 ataccgtaaa cgatcaaaac aatggcaact ggtaagaact tgtggaaatt tgcttctaac
    24541 caatcatcca aaggcaaacc aatgatgatg gctggtatgc aagcaacgat cactttaaac
    24601 caaagtgtcc acgtatcttt tttttctacc gttgatttac ttggagaaaa tgggtttaac
    24661 ttgtgaaaat aaagaacgac gacagccaag atcgccccta attggataac gacattgaac
    24721 atcgacataa attgtgaact ggcatccagt ttgatgaatt catctgctag gatcaaatga
    24781 ccagtactac tgatgggcag ccattccgtt attccttcaa taataccgag aatcactgct
    24841 ttgataatat ttgcaaaaaa cataaattcc ttccttccca attaagatac atcttatagt
    24901 ataccgttat tttttgagaa acccaatctg ttttttaata ttaagaaaaa cttgagaaat
    24961 aaagcagata agtacacgaa gagaaaaaag aagacaaagc cctcgttcag actttgcctt
    25021 ttatgattct ctggtaaaaa ttaaaatatc cccattatag aaacgagtcg cgactatgtg
    25081 ttttcccgtt atcgatataa gacgtatcat tgatgccgtg gatagaaaat cgaccgtttg
    25141 cataagtgac ctttgttacg ctgccattta aaagcccagc acgaacaggc tgtttaggat
    25201 cgatcagatt aagcaaaaag gcgatcgtca aaccatgaga tacgactaaa acatttccac
    25261 caccattttt ttcgcagtga tgggcgatat cttcaaatcc tgtccataca cggtctcgca
    25321 atgtttcata agtttccgcc cacccagcgg tatctaagtt gtatacagca ttagcaattt
    25381 gttcaaaaga aacttctgtg gtaaacattt catcatatgt tttaaaattc aatacacgag
    25441 ggatcacacc ccacatttcc atgtcatatc caccttcaag agacccgaaa caccattctc
    25501 taatcctttt ttcaataaaa taaggaattt cttttccctt agaatgttct cctagaataa
    25561 tacgggcagt ttcgattgca cgaccactgt cactagaata agcaaatgaa aagtcgacat
    25621 tttttaaccc gcgacctaaa taatgaatgc cttcttgtcc ttgctttgtg aggggtgtat
    25681 cacaccagcc ttgtactcgt tcaattgtat tgaacatcgt ttttccgtgt cgtacgatat
    25741 aaagtgtagt ttctgtcatt ttattctcct cctaagtcgt tttaacgatt gaaaaaagga
    25801 tttgtccttt tttcctgttc aatcgtcgtt gcatcgccat gtcctggata gactgcaaat
    25861 tcatcaggta atacaaacag ctgggtccga atactatgaa gtaattgttc taaattccct
    25921 gtatacagat cggttctacc gatacttcct ctaaacaggg cgtcgccaga aacgacaaaa
    25981 tcatcaaaaa taaagctgac gcttcctata gaatgccctg gggtagggac gacgcgaaaa
    26041 gacatgtttc ctaaagtata agttttcatt tcaaattcat attctgcagg cgagacaata
    26101 acatcagcaa tatcatcatg tctaccgagt ccagaaagat tcaaaatcgg atcacctagc
    26161 catttttgct ctagtgggct gacataaact gggatttgat aatacgcgcg taaaggttcc
    26221 actgcaccga tatgatcgta gtgagtatgg gtaagcaaaa tcgctaccgg ctgttggttt
    26281 gtttttttga tttcttgttt gattcgttcc gcatcttctc cagggtcaat gatcaaaagt
    26341 gcttcttcgt tgtagaccaa ataacagttt tcttcaatca ttcctgtttt taatctttcg
    26401 atatgcatgt gcattcctcc ttgatagcca aaacttcttt catccattag tataacgcat
    26461 tcattgctag gaaacaaaaa caggtactta tttctcttta cttatctgaa ttatcagact
    26521 atatgaaaag tatttggata ttctaaagag aaggaaagta agttttcata gcattttaat
    26581 atgtccttgt tacattagat tcatccaaaa atccaaattc tatatcctat atccctgcct
    26641 aatcagcagg gtgttttttt atgtttggaa agagagctct ttttgctaaa ctaggttttt
    26701 tcaggtaatc ttagagagaa agaggtggtg gaaatgatca aacagtcaaa aatatctgtg
    26761 cggcacttag tagaatttat tttgcgacgt ggaagtatcg acaatcgtaa aaaaagtaac
    26821 catactgcat tagaaggagc gagaatccac cgcaagctcc aaaaagatgc gggagaaaac
    26881 tatgaaaaag aagtcttttt aaaaacaaca gttcaattag atgaatatta cctgatcgtt
    26941 gaaggccgtg cagacggtat tttcaaaaga gacggtattt attatataga tgaaatcaaa
    27001 acctccgaac cacgatttga agatttagaa ccagaacaag tggaactttt ctttcatcaa
    27061 gcaagggtgt atgcctatat ctatagtcaa gaacatgatt tacaagaaat aaatctgcaa
    27121 ttgacttact tccagacggc agaagaagtg atcacgagaa aggttgaaca tcagacaaga
    27181 gagcagttag aaactttttt taaaaagctg actgaagatt ataaagaatg gcttgttttt
    27241 caagaaaact ggcgcactgt acgcaatgcc tctttgatgg ctttgaaatt cccccatgat
    27301 gagtatagga aaggtcagcg tgaactagcg gtagctgctt ataaaacgat ccgtacgaaa
    27361 cagaaattgt ttgtagaagc acctaccgga acaggaaaaa caatctctac gttattcccc
    27421 gctttgaaag ctgtagggga agaagaggga gaaaagatct tttatctaac ggctaaaaca
    27481 ataactagac aagtagctga agatgctatg acagcattaa aagatacagg agctgaagta
    27541 aaaagtgtca ctttgacggc gaaagacaag atctgctttt tagatgagac gacctgtaat
    27601 ccagatcagt gcccatatgc caatggttat tacaatcgga ttaatgaagg tttatgggat
    27661 cttttgaatc atgagaatca gatcactcgt gaagtcatcg aaacttatgc aagaaagcat
    27721 acactttgtc ctttcgaact atcattagat gtcagtatct ggtgtgacgt aatcattggg
    27781 gattacaatt atttatttga tccaactgtt tatttaagac gtttttttga agatgaaaaa
    27841 aacgaggact atctgttttt gattgacgaa gcacataatt tggtgaatcg ttcccgagag
    27901 atgtattcgg cagaactatc ttatgaaaaa acgaaacgaa gtaaggaagc tataccaaag
    27961 gaattcaaaa aacttcatcg ccgtttcaac aaactattaa aagaatttga ttcgatccga
    28021 gaaatagcaa aagaagatca ttgggattat catcatcaaa aagcaccggc tgaatcttta
    28081 gtaaaggcag gttatcaatt aagcgaaaaa attaaagaat ggctggcaga atttcctgag
    28141 cacccatcac aagaacaatt gcttccttat tattttgatt tgcttcattt tttgaaggtc
    28201 agtgaatttt atgatgatca ctacgaaaca acagtcgaaa aaacgtatcg cgatctgata
    28261 gtaaaagagt tttgtattga tccaagcttg tttcttgagc agtcgttgga taaaggaaaa
    28321 agcagtattc ttttttctgc tagtttttca ccactctctt attatcagga aacactgggg
    28381 ggacaaaaaa gtctggctta caaactgccg agtcctttcc cagaaggaaa ccagcaagtt
    28441 ttcatcgcaa attacctaga gacgacttat cgaaaaaggg aacacagtct ggcaaaatta
    28501 gtagagacga ttcatgcttt tgctgatgga aaggtcggga attatttcgt tttctttcct
    28561 tcttatcaat atttagatct tgcggtggaa gcattcagac agcaatatcc cgacagcaat
    28621 gtgctaatcc aagaaacgga catgaatgag gaagaacgcg aacgattttt aacaaaattt
    28681 caaacaaatc ccgctgaaac attgattgga ttttgcgtac ttggcgggat tttttcagaa
    28741 ggaatcgatt tgagagggga ccgattgatc ggcagtatga tcgtaggtgt aggtttgccg
    28801 cagatgaatc atgagcagga actgatcaaa tcatattatg atgagaagga acaccttgga
    28861 tttgcttacg cttatcagct tcctggaatg aacaaagtct tgcaagctgg tggtcgagtg
    28921 attcgtggca tggcggatca aggaatcgtt gtattggcag atcaacgatt ttctagtttt
    28981 tcttatcgac gtttattccc acaacattgg caaacagcaa gagaagtacg aagtgttggg
    29041 gaattggaag aaggaataca gcgtttttgg ttatcaaaag aagcagtaaa tcaaaaaaac
    29101 gaggaatgac agacgtcagc ctcgtttttt gattaacgat agttagtgaa ttgcaactcg
    29161 attggtaagt caagtttacg taataaagca atcacagctt gtagatcatc acggttctta
    29221 cctgtcactc taatctgatc ttcttggatt tgtgctttta ctttcagttt ttcatttttg
    29281 atagctgtag tgattttctt cgcgttttca cgatcgattc cgttgaccaa atcggcagtt
    29341 tgtcgagctt tgccacctag agcgtgttct gattcggaaa aatggatatt tttgattggc
    29401 acatttcttt tgatcagctt accaaataat acatctttga tctgttcgat tttgaaatca
    29461 tcatcgctca atacaactag attgctgttc tctaatttga tttctactgt agaatccttg
    29521 aagtcaaagc gattggacaa ttcttttgtt gtttgctgaa tcgcattttt tacttcttct
    29581 gtattgactt ctgatacaat gtcgaagcta gcatcttttg ccataggaac cctcctgata
    29641 tacatataaa tatttaccat acatctatct tatcatagga ttagggcaga ttcttacctg
    29701 tttgctaaaa ttcttctcat ttgagttcga agtattttat tttaatagat aaactgttat
    29761 tcttacattt ggaataccaa agtaggggag aggtgcttgt gagtaaaatt atgactgcgt
    29821 atcataaaaa tgaaagatta aagaaaatag tggttatttt gataaccgga ttgaccgctg
    29881 cggtcgcttt aaattatttt ttgattccag ccaatgtctt ttctgcaggg atgaatggga
    29941 tcgctcaaat catttcctcg cttttatatg aatggtttca tatcaagata gacacaggaa
    30001 catttatttt tcttttgaat atccctgtgt ttgtattagg gtttctcaaa gtaggaaaac
    30061 gagcaacgat ccttagtttt gtgaatgttg tatgtgtatc ggtgatgacg atgctgcttc
    30121 cagtcggaca gatcactgat aatattttga tgaatgccct agtaggcggt gttttattag
    30181 gtcttggcgt aggattatct ttaaaaatgg gctttacaac tggtgggatg gatatcatct
    30241 ctcttgtact ttcacaaaca actggaaaaa cagtcggaaa ctacatgctg attctaaatg
    30301 gtatcattgt agtcatcgca ggcttcttct ttaactggga aagtgccttg tatacgatta
    30361 tttccattta tgcaatgact caagtaattg atgccatcca cacgagtcat caaaaagtaa
    30421 cagcaatgat cgttaccgtc aagccagaag aggtaacaga agaactagca aaacggatgg
    30481 tacgtggaat gacattactg ccgtctgtcg gtggctattc aaaaaaacaa gggaacatga
    30541 tcatgatggt catcacccgc tacgaaatgt atgatcttga tcagatcgtt catgaaatcg
    30601 acgacgatgc atttatcaat attttaccaa ctcattcagt atttggccgg tttgcaaatg
    30661 aaaacgaaca aaaaatgtat cgaacaactg gcgtattgcc agaaataaaa aatcgttcac
    30721 aaaaaaaatg aggaatcagg tttattttag aataaattta atctattaca tctaataaga
    30781 acatttttat aaatttaaac gagaatctta cttctaaatt ctcatttaca agcagctaaa
    30841 aaaagagtat gataaataaa aatcgtgtta aggagtgtcg tacgatgagc gaagcaaacc
    30901 aagaacggtc tggacaagaa atagaactaa tcaaagaacg tattcttgct gccttggaaa
    30961 tggtgattga tcctgaatta ggaatcgaca tcgtcaattt aggcttgatt tatgatatag
    31021 aattcaatcc tgaaaatgga gaaacagtca ttaagatgac tctaacaact atgggctgtc
    31081 cattagcaga tattttaaca gaatctatcc atgacgcatt aaaagaagtg ccagaagtaa
    31141 cgaaagcaga agtcaaacta gtctggtatc cagcatggac gacagataag atgagccgtt
    31201 atgcacggat tgcgttgggg atacgataga aatcttggct gactagctat gagaagcaaa
    31261 taggaaaaac aaaaaagcct tttatccttt ctagaagaaa agagataaaa ggtttctttg
    31321 tttataaaat tttgtctgct atattataag ttaattaaat aaatgtgttc tattgataaa
    31381 ggacattcaa atccctttcc ttttggaatc cttctttttt acatcagttt gattttcgta
    31441 tagtattctt tctcatcttc acgataaatc gcagccccat ttttttgttg tgctttaaga
    31501 ctgccaagat tttttttatt tacagacaaa agtgcctcat caatgccgat tttttttgcc
    31561 tcttgtagta ctaatgctaa tccttgtgtg gcatatcctt ttttacgata attaggggag
    31621 atgccgtaac caatatggcc agcgccgttt cttaatgttt cgttcagttt atggcgcaaa
    31681 ttaaaaattc ctacatactc atgttcatca ctttctaaaa taaagacggt tgcgggaacg
    31741 taactctcag gaagatcgat tcctaaagac gttttttcgc atttttttac ataatctaaa
    31801 aattcagcaa atgtatagcc ataagcattg ttgataaatc cactctcatt tggtccaaag
    31861 ctagtttgta gctgataagc tttctttaaa tctgcttccc atagtttgat cagtttcatc
    31921 gtattcacct ctttaagtaa ccttattcat ttgttttcta ctatagcact attgttgtaa
    31981 atagccatgc ctatttttat ttattgtgtc atatcgtatt aagaatcatc gaacaataca
    32041 aatatgaatt acaataaaaa aattatttga agttcgttca ttcgttactt atgatctcta
    32101 gaagaaagaa aacgtgaatt acttcagcgt ctagaattcc agaaggatag taatcaacag
    32161 aagaaataat caatatccaa atgaaagaag atagctgaat cgagaaaaag aggaaaaaag
    32221 aaatgaagaa aaaaataaaa tagttactaa tcccagagtc gttcgtttaa cattgaaatc
    32281 gatcatatgg ataaaacaga ttttttcacc aaccctcaat agatgatgga gttgctcgga
    32341 tgaattattt gtgtagatgg cataagggag ggcgtacaga aatgaaaaag aacaaaaata
    32401 tgttcttgac aaaaaacaca tgtaatccta aactaaaaga tgtgatgcgg ctatggcgga
    32461 attggcagac gcgctagctt caggcgctag tgagggtgac ctcgtggaag ttcgagtctt
    32521 cttagccgca taatttgttt ttttataaag agaaggtaca cctggagcga tgagaaagaa
    32581 aataacatat tatctaaaag aatataggaa aaattttaaa caacggaacc agattcaaat
    32641 aaatcaaaaa aaatttgtgt gaaggtggga cagtttaaaa cctaatagat tgattcaaat
    32701 agtagattta tagattctga tacaagaaat actggcaaac gatagttaag gaataaagac
    32761 taaacttaaa ctcaaaaaga ggtcatgcga aagaataaat cgcatgacct cttactatat
    32821 tttgaatgtg tttaaacgtt caaataatga atcttagtac caaccgtttg cttcccagaa
    32881 tgctttcgcc gcttcccaag aaccataacg tgaagtaaca tattgttctg ctactctttc
    32941 ttggtttgca gcagagtagt caccatttaa gtaagaagaa tctaattggt aacggccgat
    33001 gtaacgtccg ttcgttgctg tgtaagaacc gcttgattct ttttgtgcga tccattcttt
    33061 tgcagagctt gtacttgctg gagtagcagc agtttctgtt acagcaggag caggttgtgt
    33121 ttcagcaaca ggcgctgctt gtgtttccgt ttgaaccgtt tcttgtacaa caggttgttc
    33181 agcttgaaca ggttcagctt ggactggtgc ttcttgaacg gctgcttgag gttcagcagc
    33241 tgcttgtgca gagccttctg ttgggattgt taattgttgt cctgagta
//
LOCUS       DAIGOK010000015        32410 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.015, whole genome shotgun sequence.
ACCESSION   DAIGOK010000015 DAIGOK010000000
VERSION     DAIGOK010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.015"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(<1..649)
                     /locus_tag="NOA01_000541"
     CDS             complement(<1..649)
                     /locus_tag="NOA01_000541"
                     /inference="COORDINATES: protein motif:HMM:NF014051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HCI0631766.1"
                     /translation="MNRLIKNYAYNLCYQILVMILPIITTPYVSRVLKAEGVGEFNYS
                     YAIVSVAVIIAQLGTNMYGQREIAYVQNDKIRRSGVFWSIFVIRIISSFIVFPVYILI
                     SATLHSYTVLLISMSVYLVANLFDVSWFFQGMEDFKKTAIRSMVVKLIGLVLIFLLVK
                     NENDVVLYSVILAGTELLGNALLCCHIPKYVRRISIHDISITPHIRPILGLFLPTA"
     gene            complement(651..1964)
                     /locus_tag="NOA01_000542"
     CDS             complement(651..1964)
                     /locus_tag="NOA01_000542"
                     /inference="COORDINATES: protein motif:HMM:TIGR04370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide repeat unit polymerase"
                     /protein_id="HCI0631767.1"
                     /translation="MIYQLLIVEFILILIMYILSNRCFISPAFIAIALFFLATCCVVY
                     NIEFWNVQYSKKTLWFITTGFLAMCIPEMYILFLDYLSKKRVDNYHQNQYKIEYVQIY
                     PSRQLNLIFALLSVVFFGVLLVEILRKGDYSTLGLYAIGAAKSDETQNSVIARFGISL
                     ITGLNLAYLYVFINNVIRCKERVLSNIKYLFPTLMYILCMFISGNRVFLVKLFAIVFL
                     IYIVMKSDVSPRGRVPVMQVVRKTLFVAVAILAVFYALRGITKVNSTAATREFSDYIT
                     YYIGSPVYMLDKYLQRYSPELNWGAKSLTSLVEMFGIEPEFKNAYIHVGGDSNFAGNE
                     FSWFQRPYSDFGWLGMLVFTFIVFWIFSWNIHIRINKEKNRRRRKYYILITAYFFYIP
                     VMSFYYCQMCFAFSITNIFSVVVACLLVREFTKRQDEIIFKIKEE"
     gene            complement(1965..3110)
                     /locus_tag="NOA01_000543"
     CDS             complement(1965..3110)
                     /locus_tag="NOA01_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009854067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HCI0631768.1"
                     /translation="MKKARVLAFYLPQFHPVRENDIMWGKGFTEWTNVAKAKPLFKNH
                     YQPQIPADLGFYDLRISEVRKQQAEMAKKYGVEGFCYWHYWFGNGKRILELPFNEVLQ
                     SGEPDFPFCLGWANHSWSNKTWQKSKHFTKDVVFLEQTYPGDEDYILHFKSILPALKD
                     DRYIKVDGKPLFLVFAPDSIPDSAHFISLWNKLAKENGFEGIYFVARVTSVDNKFDLS
                     SMTDVAVHNRFQRFLDMGYDAINSDMQRYAELKISGYWKRGIKSIMRKTVGNFSANLY
                     PYDKIMENFYCEEEKRECVHPQLIPRLDRTPRSGKDARVYVDSTPEKFGNAIDRALSY
                     VQDKEPEHRILFLQAWNEWGEGNYMEPDLVFGCEYLEILRKKILGEE"
     gene            complement(3107..3913)
                     /locus_tag="NOA01_000544"
     CDS             complement(3107..3913)
                     /locus_tag="NOA01_000544"
                     /inference="COORDINATES: protein motif:HMM:NF014075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HCI0631769.1"
                     /translation="MVNIKIRKVIHRIPPILKLMRFVVDVQNKYFYDKFCKNTDNIQR
                     IEKLRNKFLGETCFIVGNGPSLTMEDLELIKDKDCFAANLIFKIFDKTQWRPKYYFIQ
                     DRYADTGNELDDIDLEYMFIGDYYWRVRGMHNPNAICIHSEQSVGGKNIKFSEDISKS
                     IVSHETVTYSMIQAAVYMGYKKIYLLGMDHSYALTYNADGTVSEDKTIRSHFFEDKNA
                     NEVIASIVGMNQAYIRAREFCESNNIKIYNATRGGKLNWFDRITLEEAVK"
     gene            complement(3972..4160)
                     /locus_tag="NOA01_000545"
     CDS             complement(3972..4160)
                     /locus_tag="NOA01_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016396235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase family
                     protein"
                     /protein_id="HCI0631770.1"
                     /translation="MVVKGTSDDFYHANDEGGLAWNDPEIGIEWPQVLGEYKGNASAE
                     GYIFEDDTPLNISEKGQR"
     gene            complement(4508..4642)
                     /locus_tag="NOA01_000546"
     CDS             complement(4508..4642)
                     /locus_tag="NOA01_000546"
                     /inference="COORDINATES: protein motif:HMM:NF013621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HCI0631771.1"
                     /translation="MKEKVIEYTTGVYDMFHIGHLNVMRNAKAQCDFLIVGVSTDEPV
                     "
     gene            complement(4983..6116)
                     /locus_tag="NOA01_000547"
     CDS             complement(4983..6116)
                     /locus_tag="NOA01_000547"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012744.1,HMM:NF024831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCI0631772.1"
                     /translation="MRQKIRVLHIAQAAGGVDCYIRMLLKYLDKEKFENILICSQDFC
                     ETDYANLVLSFKQIKLTREIDLNDLKAIVIIRKLIKKYNPDIVYAHSSKAGAITRVAK
                     IGLKNYCVYNPHGWAFNMRCSVKKKMLYTAIEKIAALFCDKIICISDAEKQSALKKRI
                     CCEDKLQVIFNGVDIEAYRNKAHGEVKRESLNIPQDAFVVGMVGRISPQKAPEIFIKM
                     ARLVKNKIPEAHFIIVGNGEQKALIEKYAAEHGFLESLHITGWVNNSISYIELFDVAC
                     LLSRWEGFGLALPEYMMVGKPIIATNIDAIPNLISNGKNGLLVPVDDETAACEAVFKI
                     FSDKAMAARLTSEATSVVLEKYNSKRVASEHAVLFEMLVKKRG"
     gene            complement(6028..6580)
                     /locus_tag="NOA01_000548"
                     /pseudo
     CDS             complement(6028..6580)
                     /locus_tag="NOA01_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350621.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
     gene            complement(7049..7738)
                     /locus_tag="NOA01_000549"
     CDS             complement(7049..7738)
                     /locus_tag="NOA01_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCI0631773.1"
                     /translation="MESEDLLSRISKVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYAAYRIRKEEPGSPIFFSHTRVGKNGKTFKMYKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTQIGKIIRAKSIDELPQLWNVLKGDMSLVGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSGRNDVSFHAMVDLDLEYITNQSLLNDFKLIIKT
                     IWIVIKPNGAY"
     gene            complement(7792..8556)
                     /locus_tag="NOA01_000550"
     CDS             complement(7792..8556)
                     /locus_tag="NOA01_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HCI0631774.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIVMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIQRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLELGKTPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPIYEEVKKKKFGLF"
     gene            complement(8584..9282)
                     /locus_tag="NOA01_000551"
     CDS             complement(8584..9282)
                     /locus_tag="NOA01_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HCI0631775.1"
                     /translation="MARTQKQKATPTHAVSLITLTNPSSPIAEQYRTIRTNIQFASTT
                     GQQIKTIVVTSSGPGEGKSTTASNIAVVFAKSGQRVLLVDADLRKPVIYKTFQLNNAS
                     GLSTALSSSGSVADVIQRAPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPIVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLEMVQARVLGVVYN
                     GAEHSKDDGYYYGN"
     gene            complement(9295..10074)
                     /locus_tag="NOA01_000552"
     CDS             complement(9295..10074)
                     /locus_tag="NOA01_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HCI0631776.1"
                     /translation="MEETISLEEIFETLRKHLITIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVQERLKTEDNVKMTP
                     EQIKDAISINQSENSQMFSIQAISTSAMTAQQIANTTSQVFQENAKDVMNVDKISIVS
                     GAVANTTPISPNNKLNLVIGLMLGMMVGVVLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSEKELKAGIRKKGLAPTRPKVTNDKTASSRRSRSRV"
     gene            complement(10090..11034)
                     /locus_tag="NOA01_000553"
     CDS             complement(10090..11034)
                     /locus_tag="NOA01_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCI0631777.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLNKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGVQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSILNALESNMKTDMSFDEMKKIALDYREAFDTIKQD
                     QMQGEGFMQDDISYQKVNDNELDRVKKELKEQMNLENK"
     gene            complement(11071..11847)
                     /locus_tag="NOA01_000554"
     CDS             complement(11071..11847)
                     /locus_tag="NOA01_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631778.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHFNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKIRAAYATKAQTVAQQMGYSNIS"
     gene            complement(12232..14307)
                     /locus_tag="NOA01_000555"
     CDS             complement(12232..14307)
                     /locus_tag="NOA01_000555"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCI0631779.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKESKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(14309..15226)
                     /gene="glyQ"
                     /locus_tag="NOA01_000556"
     CDS             complement(14309..15226)
                     /gene="glyQ"
                     /locus_tag="NOA01_000556"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCI0631780.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(15612..16424)
                     /gene="recO"
                     /locus_tag="NOA01_000557"
     CDS             complement(15612..16424)
                     /gene="recO"
                     /locus_tag="NOA01_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCI0631781.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTSEKDMSEKDLPKKEE"
     gene            complement(16567..17466)
                     /gene="era"
                     /locus_tag="NOA01_000558"
     CDS             complement(16567..17466)
                     /gene="era"
                     /locus_tag="NOA01_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCI0631782.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(17481..17876)
                     /locus_tag="NOA01_000559"
     CDS             complement(17481..17876)
                     /locus_tag="NOA01_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HCI0631783.1"
                     /translation="MDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFSVIVGLILFVPKIYHLLFN"
     gene            complement(17860..18336)
                     /gene="ybeY"
                     /locus_tag="NOA01_000560"
     CDS             complement(17860..18336)
                     /gene="ybeY"
                     /locus_tag="NOA01_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCI0631784.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(18353..20545)
                     /locus_tag="NOA01_000561"
     CDS             complement(18353..20545)
                     /locus_tag="NOA01_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HCI0631785.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(20569..21540)
                     /locus_tag="NOA01_000562"
     CDS             complement(20569..21540)
                     /locus_tag="NOA01_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCI0631786.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            22246..23625
                     /gene="brnQ"
                     /locus_tag="NOA01_000563"
     CDS             22246..23625
                     /gene="brnQ"
                     /locus_tag="NOA01_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCI0631787.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(23781..24227)
                     /locus_tag="NOA01_000564"
     CDS             complement(23781..24227)
                     /locus_tag="NOA01_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HCI0631788.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(24254..24430)
                     /locus_tag="NOA01_000565"
     CDS             complement(24254..24430)
                     /locus_tag="NOA01_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCI0631789.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(24592..25020)
                     /locus_tag="NOA01_000566"
     CDS             complement(24592..25020)
                     /locus_tag="NOA01_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCI0631790.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            25112..25972
                     /locus_tag="NOA01_000567"
     CDS             25112..25972
                     /locus_tag="NOA01_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HCI0631791.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(26066..26416)
                     /locus_tag="NOA01_000568"
     CDS             complement(26066..26416)
                     /locus_tag="NOA01_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCI0631792.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(26432..28159)
                     /locus_tag="NOA01_000569"
     CDS             complement(26432..28159)
                     /locus_tag="NOA01_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HCI0631793.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLIDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            complement(28373..29299)
                     /gene="trxB"
                     /locus_tag="NOA01_000570"
     CDS             complement(28373..29299)
                     /gene="trxB"
                     /locus_tag="NOA01_000570"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCI0631794.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(29444..30880)
                     /locus_tag="NOA01_000571"
     CDS             complement(29444..30880)
                     /locus_tag="NOA01_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCI0631795.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDKDHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     ENEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDTVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(30877..32280)
                     /locus_tag="NOA01_000572"
     CDS             complement(30877..32280)
                     /locus_tag="NOA01_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCI0631796.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMATMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     DLIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
BASE COUNT         9151 a         6995 c         5413 g        10851 t
ORIGIN      
        1 ccgctgttgg aagaaataat cctaatattg gtcgaatatg gggtgttatc gatatatcat
       61 gaatacttat tcttctaaca tattttggaa tatgacaaca taataatgca ttccccaaca
      121 gctcagttcc agccaatata actgagtaca atactacatc attttcattt ttcaccaata
      181 agaatatcaa aacaagacca attaacttaa ccaccatact tctgattgca gtttttttga
      241 aatcttccat cccttggaaa aaccaactga catcaaaaag atttgcaact aaataaacag
      301 acatacttat tagtaaaaca gtgtatgaat gaagagtagc cgaaataaga atatatacag
      361 gaaaaacgat aaacgaagaa ataatacgga taacaaagat gctccaaaac actccacttc
      421 ttctgatctt atcattctga acataagcaa tctcacgttg tccatacatg ttcgtaccta
      481 attgagcaat tattaccgct accgatacaa tggcgtacga ataattaaac tccccaactc
      541 cctccgcttt caatactcta gatacgtatg gagtcgtaat aataggcagt atcataacca
      601 aaatttggta acaaagatta taggcataat ttttgatcaa cctattcata ttactcctcc
      661 tttatcttaa atataatttc atcctgcctt ttcgtaaatt ctcgtactaa aaggcaagca
      721 accacaacag aaaatatatt tgttatagaa aacgcgaaac acatctggca atagtagaaa
      781 ctcatcactg gtatgtaaaa gaagtatgcc gtaattagta tatagtattt ccttcttctt
      841 ctgttttttt ccttattaat gcgaatatga atattccaag aaaaaatcca aaaaacaata
      901 aaagtaaaca ccagcatacc taaccaacca aaatcagagt aaggtcgttg aaaccacgaa
      961 aattcattcc ccgcaaagtt actatctcca ccaacatgga tatatgcgtt tttaaattct
     1021 ggttctatac caaacatctc taccaatgag gtaagacttt ttgcccccca attcaattct
     1081 ggagaatatc tctggagata tttatccagc atatatactg ggcttccgat ataataagta
     1141 atatagtctg aaaactctct tgttgccgca gtcgagttaa cttttgtaat acctcttaat
     1201 gcataaaata cagccaaaat cgctactgca acgaataaag tttttcgcac aacttgcata
     1261 acaggaactc gtccacgagg cgatacatct gacttcatta ctatgtatat caaaaatact
     1321 atagcaaaaa gcttaactaa aaacacacgg ttaccggata taaacataca taaaatatac
     1381 attaatgttg gaaacaagta ttttatattt gataaaacac gttctttgca tctaattaca
     1441 ttattgataa atacatacaa atatgcaagg tttaatcctg taattaaact gatgccaaat
     1501 cttgcaataa ctgagttctg cgtttcatct gacttagctg ctccaattgc atataatcct
     1561 aaagtgctat aatcgccttt tcttaatatt tctaccaaaa gaacaccaaa gaacacaaca
     1621 gataatagtg caaaaattaa atttaattgt ctgctagggt aaatttgtac atattcaatc
     1681 ttatattgat tttgatggta attatccact cttttttttg ataaatagtc taagaataat
     1741 atatacattt ctggtataca catagctaag aaccccgtcg taataaacca gagcgtcttc
     1801 ttgctatatt gcacattcca aaattcgata ttataaacca cacagcatgt cgccaaaaaa
     1861 aacaatgcaa tcgcgataaa tgctggtgat ataaaacatc gattagacaa aatatacatt
     1921 attaaaatta gtataaattc aacaatcaat aattgataaa tcatttattc ttctcctaat
     1981 attttctttc ggagaatctc caaatattcg catccgaaaa ccaaatctgg ctccatataa
     2041 tttccttctc cccactcatt ccaagcctga agaaatagta tccgatgctc tggttccttg
     2101 tcttgaacat acgaaagtgc tcggtctatt gcatttccaa atttttctgg agtactatca
     2161 acatacactc ttgcatcttt accagatcgt ggggttcgat ctaatcttgg aatcaactga
     2221 ggatgtacgc actctctctt ttcttcttca caataaaaat tctccattat cttatcatat
     2281 ggatagagat ttgctgaaaa attaccaact gtctttcgca ttatgctctt aatacctctt
     2341 ttccagtatc ctgaaatttt taattcggca tatctctgca tatcagaatt tattgcgtca
     2401 tatcccatat caaggaaacg ctgaaatcta ttatgcactg ctacatctgt catagatgac
     2461 aaatcgaact tattatcaac agatgttact ctagccacaa aatagatacc ttcaaatcca
     2521 ttctccttag caagtttatt ccatagtgaa ataaaatgag cagaatctgg aatagaatct
     2581 ggagcaaaaa ccaagaataa aggtttacca tcaactttta tatatctatc atccttcaat
     2641 gccggaagaa tagatttaaa gtgaagtata tagtcttcat caccaggata tgtttgttcc
     2701 aaaaacacaa catcctttgt gaaatgcttt gacttttgcc aagttttatt tgaccatgaa
     2761 tgattagccc aaccgagaca aaaaggaaag tcaggttctc cgctctgtaa cacctcatta
     2821 aatggaagtt ctagaattct cttaccattg ccaaaccaat aatgccaata gcaaaaaccc
     2881 tcaaccccat atttcttagc catctcagcc tgttgttttc ttacttccga gattcttaaa
     2941 tcatagaaac ctaaatctgc aggaatctgg ggttgataat gattcttgaa tagaggttta
     3001 gctttagcaa cattagtcca ttcagtaaag ccttttcccc acattatatc gttttcacga
     3061 acaggatgaa attgtggcaa ataaaatgcc agtacccttg cttttttcat ttaactgcct
     3121 cctctaaagt aatacgatca aaccaattca gttttccgcc acgagttgcg ttatatattt
     3181 ttatgttatt actttcgcaa aactctcttg ctcttatata tgcctgattc attccaacaa
     3241 tacttgctat tacttcattt gcatttttgt cttcaaaaaa atgacttcta atggtcttat
     3301 cttcagacac tgttccatca gcattataag tcagtgcata actatgatcc ataccaagaa
     3361 gatatatttt tttatatccc atataaacag cagcttgaat catcgaataa gtaactgttt
     3421 catgcgatac aatggattta ctgatatcct cactaaactt aatatttttg ccaccaacag
     3481 attgctctga gtgaatacat atagcatttg gattatgcat tcctctaact ctccaataat
     3541 aatccccaat gaacatgtat tctaagtcaa tatcatctaa ttcgttacca gtatctgcat
     3601 atctatcctg gataaaataa tatttaggtc tccactgggt tttatcaaaa atcttaaaaa
     3661 taagattagc agcaaaacaa tccttatcct ttattaattc caaatcctcc attgtgagac
     3721 ttggaccatt acctacaata aaacaggtct cacctaaaaa tttatttctc agtttctcaa
     3781 ttctttgaat attatctgta ttcttgcaga acttatcgta gaagtatttg ttttgaacat
     3841 caaccacaaa tctcattagc tttagaatag gcggaattct atgaatcacc tttctaattt
     3901 ttatatttac catatttact ccttccgaat tacaatatca accaaaaatt cgttatctaa
     3961 ttaaatgttt actatctctg gcctttctca ctaatattca atggagtgtc atcttcgaag
     4021 atataccctt cagctgatgc attaccttta tattcaccaa gtacctgtgg ccattcaata
     4081 ccaatctcag gatcattcca agccaagccg ccctcgtcat ttgcgtgata gaaatcatca
     4141 ctagtacctt tcacaacgat attcctttct ttattaaact tttatcaact acgataattc
     4201 aacttttgat cgagtcctca tcaatgatac tcttaattac atgagtcaac tgagtgctgg
     4261 aaataccatc tgtatgggga agataaacaa attcaacacc ggttcccata aactgttctt
     4321 catatttttc ctactgagga ttgcctttct aatcagaacc cacgaacatt ttgttaaatt
     4381 tatatttctc ccatgccgca tacttatcct tatcattttg agggacaacc atatcgacat
     4441 actttattga atttacaatt tcggctcgtt ctacatacgg aataacaggt gtcttatttt
     4501 tatcttacta aaccggttca tctgtactaa caccgacaat caaaaaatca cattgtgctt
     4561 ttgcatttcg cataacattc aaatgcccaa tatgaaacat gtcatataca cccgtggtgt
     4621 attctataac tttttctttc ataaatatct cctttaactg tgtatttcag agtaaaatag
     4681 gaatctttct catcaatctg catcacactc tacatgtcca aataacatag accgaaaagt
     4741 caagaaagat aattttccag tctgtgatga accttctctc ttctacatac ttcacataga
     4801 gttccattca ttcttcgaaa gataaatcat ttcgacgcgt aacaatcatt accatcgaac
     4861 aaaaacgttg aaccacatac aacaggcaca ttcctgcggt aatttcttta atgttttttc
     4921 tcctgagtct tcacttaccc cgatatagtt aatgcgactc aaaaaatttc ttgctttcat
     4981 cgttaacctc tctttttaac taacatttca aacaataccg catgttctga agctaccctt
     5041 ttggaattat acttttcaag cactaccgat gtagcttcgg aagttagcct tgcagccatt
     5101 gctttatccg aaaaaatctt aaaaactgct tcgcaagcag ccgtctcatc atcaacgggc
     5161 accaacaatc cattttttcc attgctaatc aaattgggaa ttgcatcaat attggtggca
     5221 atgataggtt ttcccaccat catgtactca ggcagagcca aaccaaatcc ttcccaacgg
     5281 cttaataaac atgcaacatc aaatagttca atatagctta tcgaattatt tacccagcct
     5341 gtaatatgca gactttccaa aaaaccatgt tctgctgcat atttctcgat aagtgctttt
     5401 tgctctccat taccaacaat aatgaaatgg gcctctggta ttttattttt taccaatcta
     5461 gccattttaa tgaatatttc tggagccttc tgcggactaa ttcttccaac catacctaca
     5521 acaaacgcat cttgtggtat attcaaactt tctcttttta cctctccatg agctttattt
     5581 ctatatgcct caatgtctac gccattaaaa atgacctgca acttatcttc gcaacaaatt
     5641 ctctttttca gtgctgattg tttctcggca tctgatatac agataatttt atcgcaaaac
     5701 agagcagcta ttttttctat agcagtgtat agcattttct tcttaactga gcacctcatg
     5761 ttgaatgccc atccatgtgg attgtatacg caatagttct tcaaaccaat tttagcaaca
     5821 cgtgtaatgg ctcctgcttt actcgaatgt gcatacacaa tatccggatt atatttcttt
     5881 atcagctttc taataattac gattgctttt aggtcattga gatcaatttc tctagtcaat
     5941 ttaatttgct taaatgagag cacaagattt gcataatccg tctcgcaaaa atcttgcgag
     6001 caaatcaata tgttttcaaa cttttcctta tccaaatact ttaaaagcat gcgaatataa
     6061 caatctacgc cacctgcagc ttgggcaatg tgtaaaactc ttattttttg acgcatttct
     6121 gtcctccatt ttacttatca taacaaacag tatatctcga attaccagaa aaataatctt
     6181 ccaatcagca aagaaaactc tcttatacta cttcacattg atctccatcc attctttaaa
     6241 ggaaagttca tttcaattca gagcaatctg ccaataatag ctaagatcag gctgtacata
     6301 caattgctgt ctttcatagc ctccatactg cttcatcttt ctcagcaatc cagatttggg
     6361 acccacgata ttcatatctt ccttgaaaat attccaaatc tgaggagatt catcatccac
     6421 gaacacgaaa cgaaacttat agaacttgaa caatttacca tttcttccaa ctctatcttg
     6481 tgcgaaaaca ggagtccgcc ggatcatcaa acacaataac taacgctgta atcagtatca
     6541 caggacacaa atagcaagtg ttaaaatgga aaatatgaat acttctccaa tatacccgga
     6601 ctagataact cccttttgtt cataatccct catactcatc agattcacca aactttaccg
     6661 tttatttttt tcaatagttc accaaatgac tccatctaac aaggtcaaat ataaaaaatt
     6721 aacgcatttt cattatcttt taggcattta ttaatggaga tatttagtgt ttttataaca
     6781 taataaatgc tcaatgagag cctatgttgg gtacatcaat attaaagtat tcagcactaa
     6841 tatgtttaag atcgatatta tatatttcat aattttgaaa attttctttc atataggaaa
     6901 catgatactg aattgcttta gtcacctttc tttttttaga ttttctacaa gtgattcaaa
     6961 acttccataa ttacagttat tacttttgaa acgataatat gaacatgtgt aatctttttt
     7021 atcaacaatc tatcaattct atcccttact aatatgcccc atttggtttt ataacgatcc
     7081 aaattgtctt gataattagt ttaaaatcat tcaatagact ttgattagtt atatattcta
     7141 aatctaaatc aaccattgca tgaaaactaa catcatttct accactaact tgccacaaac
     7201 ctgaacatcc aggttttaca tacaagcgtt gaagatcata tctcgtgtac tcagctactt
     7261 ccctttccaa aggtggacgt gggccaacta gactcatatc tccttttaaa acattccata
     7321 gctgtggcaa ttcatcaatg cttttagcgc ggataatttt acctatttgg gtgacacggg
     7381 gatcttcttt catcttgaac attgcgcctt ctatttcatt ttgatcaagt aactcttcca
     7441 atttttcttc agcatctaaa cacatcgaac ggaatttata cattttgaat gtttttccat
     7501 ttttaccaac acgtgtatgg ctaaaaaata tcggactccc tggctcttct tttctgattc
     7561 gataagctgc atagaacatc aaaggagaag ctaaaatcaa accaatgatg cttcctacaa
     7621 tatcaatcat tcttttaaaa aataaataga tatatcgttt ttcaactttt tctttatcca
     7681 accaaacttg attaggatgt actttactaa tacgagataa taaatcttca ctttccatac
     7741 tttcagtccc tttcgctctc tattcatttc ctttttatct acgaataaaa actaaaataa
     7801 accaaacttc ttctttttta cctcttcata tattggataa ctgatctcat ccccgttgat
     7861 cagatccttt gcgacttgct tcatctgtac tactttttcc ttaccaaagt ccttggcaat
     7921 ctgctcatag cactctttta agtaaaaggt gcgcttagag accccatggg catcagaagc
     7981 agccatctgc acgagattat gtttgaccat cgttttggct gttttctgga tatctttgcc
     8041 aaatacccca acataacttg gcgcagtgag ttgcgccaaa catcccatat ctaggaacgg
     8101 aagcaaatga ttcgggtctt cgcgaaaatg cgcgttacgt tctggatgga cgatcactgg
     8161 tgtctttcct agttctaaca actcaaaaaa aagctgctct gcatataacg gcacttccag
     8221 tgtcggaaat tcgatcagaa gatatgtatc atctaaatct gtaaataaaa tttcatctcg
     8281 ctggatgtct tcaatgagcg ttccggtaat acgtacttct tgtccttcca ataacgtcaa
     8341 aggcaattgt cttttctcta attctgcttg taacgaagcg actagagaaa tgacttgtga
     8401 tttttcattg ctgtattttc cattattgtg gtgtggcgtg cataaaatat gcgtgatgcc
     8461 ttgctgaatc gctttttcag ccattacgat acttgcctct aggttttccg ccccatcatc
     8521 gattcctggt aggatatggc aatgtagatc gatcatttct cttgcctcct ctcttcttat
     8581 ttcttaattt ccgtagtaat aaccatcgtc tttgctgtgt tctgctccgt tgtatacaac
     8641 acctaggact cgtgcttgta ccatctccaa cagctctttt gctttagtca aggattcttt
     8701 acgtgacgta ttttcacgta ccaccaataa ggtgccatcc gtttttgaag acatgatctg
     8761 tgcatctgtt acggctacta ttggtggcat atcaaaaatt accacgtcaa ataactgtct
     8821 tgcttctgct aggatctgat ccattcttgg cgaacttaac aattctgatg gattgggtgg
     8881 cttaggtcca ctaggcaaaa tcgacaagtt ctcgactggc gctcgttgga tcacatctgc
     8941 tacacttcct gatgaactaa gtgctgtact taatccactg gcattgttca gctgaaatgt
     9001 cttatagatg acgggtttac gtaaatctgc atcgactagt agaactcgtt gaccagattt
     9061 agcaaaaacg actgcaatgt ttgatgcagt ggtagatttt ccttctccag gacctgatga
     9121 tgtcacgaca atcgttttga tttgctgacc agttgtggag gcaaattgga tattcgtccg
     9181 gatcgtacga tattgttcgg cgattggtga agatgggttt gttaacgtga tcaaactgac
     9241 tgcatgagtt ggtgttgctt tttgtttctg tgttcgtgcc atttttcttc cctcctaaac
     9301 tcttgagcga cttctgcgtg atgaggctgt tttatcgttt gtaacttttg gtcgtgtagg
     9361 agccagtcct tttttacgga taccagcttt caattctttc tctgacattt ctggaactgt
     9421 tcctaagatt gggaacccta atgtatccgt cacatatttt tcatctttca ctgttcgatc
     9481 gagtaattca aataagaatg ccagaactac acctaccatc attcctaaca ttaagccaat
     9541 cactagattc aatttattat ttggcgaaat aggtgtcgta ttggcaactg cacctgaaac
     9601 gatcgagatt ttatcaacat tcatcacgtc ttttgcattc tcttggaaaa cttgtgaagt
     9661 tgtgttagcg atctgttgag cagtcattgc actcgtgctt attgcttgaa tcgagaacat
     9721 ttgtgaattt tctgattgat tgattgagat ggcatccttg atttgttcag gagtcatttt
     9781 cacgttgtct tctgttttca aacgttcttg gacttcattt agcactagat cactgacgat
     9841 catatcttta tacgtattga tcatttgcaa gttcgtattg acatcattga catttgcagt
     9901 atctgattgc ggcagtgtca caatcaactg tgcttgcgaa ctgtattttg gcgtcaaaat
     9961 gaaaaacgtt acaattcctg agatagccaa gccagcaaac atcgagatga tgattgttat
    10021 caagtgtttg cgtaatgttt caaaaatttc ttccaagctg attgtctctt ccattttttc
    10081 tccactttcc tatttattct ctaaattcat ttgttctttt aattcttttt tcacacgatc
    10141 aagttcattg tcattaactt tttgatatga gatgtcatct tgcatgaagc cttctccttg
    10201 catctgatct tgcttgatcg tatcaaatgc ttcccgataa tctaaagcaa tctttttcat
    10261 ttcatcaaag ctcatgtctg ttttcatatt cgactctaga gcattcaaaa tggattgata
    10321 gttgctgaca ccatttagac tcaatacttt ttttgctacg ccttctacga tctttctttg
    10381 gcgcccttgt ctaccgtaat caccatttgg gtcttcataa cgcatacgag aataagataa
    10441 tgcctgaacc ccgttcaatg taattttacc gattggaaag tcatagccat cttgtgagaa
    10501 agtgaggtca ttattgactt cgataccacc aactgcatca acgagttcct ttagcccttt
    10561 catattgata gagacatagt gatcgattgg gatgtctaca tatttttgta cggtatccat
    10621 cgccatggca gctccgccga atgcgtaagc gtggttgatc ttgtcttttg tgtcacgacc
    10681 tacgatgtcg acatacgtat ctcgaggaat gctgacgatc gttgtctgtt tgtcttcagg
    10741 actgactgtg gcaaccatca tcgtatctga tcttccttga tcgacccgac ctaaatctcc
    10801 agtatcaatc cccatcaata gaacagagaa agattcttgt ttgttcagat caactggttt
    10861 cgttcgttct ccttgggttc gttccactga ctcgtaagtt ttctcaatag aatgtgagag
    10921 atccagatat aattttgctc ctactcctac aattgctaat actaatacga taagaactgc
    10981 taaaataatc agaaatattt ttttccctct tgattttctt cttcgtcttg ccatagtttc
    11041 ctccgtttgc tagctttgct ttaattgtta ctaacttata ttgctatagc ccatttgctg
    11101 agcaacagtt tgtgcttttg ttgcgtatgc tgcgcgtatt ttttcattcc ggatctgctc
    11161 tatttgactg agcaccgtca aatattgatc gtaagtagcg gtggcagtca cttgaccatc
    11221 cttcaacatc gtatcgaata actgatctag ttgcgtgact tgcgcgatct gagtatcagc
    11281 gaatcctaga tattgcataa caaccgtttt ccaaggacta tctttaaaaa agctcatatt
    11341 ttcacgtaca cgagcgacat ctgcacttgt caccttttct ttgatgacga catcgtctac
    11401 agcttgctgc cagtttgata catttttagt aaataaatcg ttgacagctg tctggatctt
    11461 cagttttgat tctgcatcct ctaactgcgt taagactgct ttcttttctt cttggatcgc
    11521 agcagcttct ttaggtaatt ctgattcctt gatgtcataa tcatctgccg aaagtttgat
    11581 agaagatagt ttatgtgaca aatttgaaac tgtttcttct gttatttcag gatttaggaa
    11641 aattttttct tttgtactgt acagcttatc tacttctttt gataaagagg tcacggtgtc
    11701 tttttgattg gtgatggcag attctgcgta attgatccgt tgatgatgtt gccgctgatt
    11761 attgaaatgg tatccaaaca agcaaatcac aatagcgcct gtgcctaccg ccacagtagc
    11821 caataacttg acgaacggct tttccataga ttcactccta aatattgatg atagataaat
    11881 aaagatgtga ttcattccca tcagaatgag cgctttttat tttaaaagca taattaaact
    11941 aatatattca tagaaatatt tataaacagg ttttccctat ataatcatac cacaaaaagt
    12001 tttcttgact agggaaattc ctaatttaaa agtaaataaa acagtaacaa gatcatgatt
    12061 aatcgttttc atttttattt cctctctttt catgagcatt ttgaaaattc gaacaaagaa
    12121 taaaataaca aaaattatta aaatagaatt aataacagct atttatattt ataacaaaaa
    12181 aacagagaca cagcacgttt cgtgcatgct ctctgttttt caaacaaaag actattttgt
    12241 gatgatttga tccaagcttg ctaatgacaa agccatggca gctatttgac taagttgtgt
    12301 tagtcggttc ttacgtactg ctggttcatc agccatgacc atcgtttgat caaaataatt
    12361 ttcgatcagt ggacgaagag cgacaagctg gtcgtagttt tcgtggatcg tttggtcagc
    12421 aaaacttgct ttcattgctt ctactgcttt gtgtaattct ttctctgcgt cgttttcgaa
    12481 cagctcagga tctacttctg attgtttttc aagctgcgct tttttcgcta agttcacgac
    12541 acgtgtcaaa gcttccattg aaggtttgaa gtcttcatct gcaagatgtt gtttgaacaa
    12601 ttgtgctgct gcaaatactt ttgtcagatc tttttgttcc gcattcaaaa cagcatcgat
    12661 gacatcatga cgaatattct tcgttgtcaa taattggcgc agacggcctt tgataaagtc
    12721 gatcacttgt tgttgccctt ctgataaacc aatgccgaaa cgatccgtat ctttactgat
    12781 aacatcgcta atcgtttctt gcagaacaga caaaggaaag gcccattttt tcgattcgat
    12841 gatccgaacg acaccgtatg cttggcgacg taaggcataa ggatcgtttg aaccgcttgg
    12901 gatcatccca acagcaaaga aagaaaagat gctatctaat ttatctgcaa tcgctaaaac
    12961 agaacctact gtagactgag gtagctctcc ttcactggat ataggcatgt aatgctcacg
    13021 aatcgctgtc gctacagcag gtttctcgcc tttcagcaga gcatattttt cgcccatgat
    13081 cccttgcagt tcaggaaatt cccctaccat gttggttact agatcaaatt tatagatttc
    13141 agcagctcgt tgcagatcca tcaattcttc ctcagatagt cctacttgtt cgccaatgat
    13201 acgggcaatg acttgtaccc gttgcatttt ctcatagata ctaccgattt tttcatggaa
    13261 agtaacagtt tttagttttt caacgtattg gtcgattgaa gctttcttgt cttcattata
    13321 aaagaattcc gcatcttcta aacgagccgt caatactttt tgattgcctc gaaccacatt
    13381 ttccaatttt acccgatcac cattacgagc agaaataaaa tgaggaagca gcataccttg
    13441 atcattccgt acctcgaaat aacgttgatg ttctttcata gaagtgacta gtacttcttc
    13501 aggaacagaa agatattttt cttcgaagtc gccagaaaag acagttggat attcgactaa
    13561 attgttgact tcttctagta aatcttcgtc tagatcaata acccaatgat ttttttcggc
    13621 taattcattg gcttgtgtaa cgatcatttc ttgtcttttt tgcggagacg caatgacgaa
    13681 ttgttcattc aatttttctt catattcttt tgcatgcgca aatgtcacat cttcgcctaa
    13741 gaaacgatga cctcgagagg ctctgcctgt tttgacatcc aagatttcga atgggattac
    13801 ttcgtcatct aacaatgcca cgatccagtg aatagggcgg atatattcaa aatcgtattc
    13861 tgcccaatgc atcgttacag ggaaagtcag gcttttaatg acatctgcta aacctgtcaa
    13921 tacttctttg gattccttac catgagtgta tttcgttaca tagacatatt ccacaccttt
    13981 taattctcta aaagtgatag cgtctgtcgt cagaccttgt ccgcggacaa atccttccgc
    14041 tgctttcgtc cagtttccat ctgcgtccaa agcaattttt ttggctggtc ctttgacttc
    14101 ttcttcactg tccgcttgtc gttctggaat gtttgttact ttgattgcta aacggcgcgg
    14161 tgtggaaaat gtttcgatcg tatcataagt caattggttt tcatctaaaa attttgctgt
    14221 tttttcttct aattgtttca tgcttggtgt gacaacatga gcaggcattt cttccagccc
    14281 gatttcaagt aataagtctt ttgccatctt attcagcctc cttcacgttt tctggatttg
    14341 ctgtgttttt attcaacaaa gggaatccta atttttcgcg ttctgccaca aagattttgg
    14401 ctacagcacg agccatgttg cggatgcgag aaagataacc ggcacgctct gttacggata
    14461 ctgcaccgcg ggcgtctaat aagttgaatg tatggctaca tttcaagata taatcatacg
    14521 ccggatgaac aagattttca tcgatacagc gtttagcttc tttttcaaaa cgatcaaagt
    14581 tgcttagtag caagtcttgg tcgcttactt caaaagaata tttagaatgt tcatattctg
    14641 gttgtttgaa gatctcgcca tatttcacgc catcagccca ttctaaatca tagacacttt
    14701 caacttcttg gatataagaa gcaagacgtt ctaatccata agtgatctca gaagttactg
    14761 gtttacaagg cagtccacca acttgttgga agtacgtgaa ttgtgtgatt tccattccgt
    14821 caagccatac ttcccaaccc acaccggcac agcccatgga tgggttctcc cagttgtctt
    14881 caacaaaacg gatgtcgtgt tctagtggat cgattcctaa aaggcgcaag ctttctaaat
    14941 aaagctcttg gatattctca ggtgaaggtt tcatcacgac ttggaattga tgatgctgat
    15001 acaaacggtt tgggttttcg ccataacgtc catcagctgg tcggcgagat ggctctacgt
    15061 aagcagcatt ccatggttct ggtccgattg cccgtaaaaa ggtatatggg ctcatcgtac
    15121 ctgctccttt ttctgtgtca tacgcttgca tcagcatgca gccgtttgat gaccaaaatt
    15181 tttgtaatgt taaaatcata tcttggacgg tcaatttttt actcatctgt tttgcccctt
    15241 tcaattttaa agcgttgctg tcaaataaga atacaggaaa gaaaacgaca aaaaatccct
    15301 atgtctgatt acagacatag ggacgaataa ttcgcggttc caccctactt ccgaataaag
    15361 attcggcagc ttcatttatc gtactcgcaa gtgcctcttc aaaaatcgtt tgggtccggc
    15421 tcccactctc ccgaactcgc taaaccacgt gttttttact gatcttggtc aatgtacgta
    15481 ttcattttgc agttttcttg tttctataat agatcctctc agtgaacgat ctatttgatc
    15541 tacggttaat aatagacgga actccctgtg tttgtcaaat ggttttgatt ttttgttgta
    15601 tgtttcattt actattcttc ttttttaggt agatcttttt cagacatgtc tttctctgac
    15661 gtctttaata cgtgtgccca agacttcatt tgatcaatga actttttgct tttcaaatgg
    15721 ataccgacat attcatcata cagctggtcg atgagttcac ggattgcttg tttggtttct
    15781 tctttcagat ggattccact gattttatca taagaaatag ctgaaaacat ccgaacgaaa
    15841 taaacagctc gaggatctgc atgataacgg tgatcatcca tcgaccagtg tctttcgcat
    15901 atcacaccac catatttgga agaatagtca aattttcctt ttgtttcccc acatacgacg
    15961 cagtgtgtcc agattggtgc aattccgaag cgttgcagta tttggacttc aaaaatattc
    16021 gtgatgattt ctgcatcatc tccttggtcc atccgttgca gtgcttgttg cgtaaactgg
    16081 aacaaatgcg ggtcgtactg atgatcttcg attgccgcat ctactagatt caataaatat
    16141 gtgccatagc cgctcaaaaa gatgtcttct tggatgtgtc gaaacgcgtg gacatctttg
    16201 acactgctta aaaatgacaa accttcactt cgaaaattcc cgatataaac tgcttcagta
    16261 aaagggagca aagccggagc taagggctga ttttgccgat ggatcccttt gatataaaac
    16321 atcattttgc cagaagattc cgtaaagatt ttgactaatt tatctttttc tttgtaatta
    16381 cggctagata aaatcaagcc ttttgattct gcaagctggc tcatattctc cctcctaaaa
    16441 aataaatctg gaaaagcaga ttctgccgat ccagatcatt tatgttgcga tcttccgcag
    16501 cttttctcaa cacgcggatt tttatccagt ttatttctat acctaactta caagaagctc
    16561 agaatatcaa tattcgtctt ttcggtagcc gaagtcttgc agatagactt ttttgtctcg
    16621 ccagtctttt tgaactttga cccaaagttc tagaaagact ttgtctccta aaagattttc
    16681 gatatctttt ctcgctttcg ttcctacttg cttcagcatt tttccgcctt tgccgataat
    16741 gatccctttt tggctatctc tttcgacaat aatcgtagcc tggatatgga ttttatcatt
    16801 ctcgtttcgc ttcatcgaat caacaacgac tgcgacagaa tgaggcactt cgtctcgtgt
    16861 taaaaacaag actttctcac ggatcaattc agaaacgatg aagtattcag gatgatcagt
    16921 cacttgatca tctggaaaat attgtgggcc ctctggcatt tcactgacta atacatccat
    16981 caaacgttcg acgttgtttc cttctgtagc agaaattgga atcacttgtg caaacggcat
    17041 ctgcgttgaa taatcttcga tgattcccaa caactcgtct ggatggattt tatcgatttt
    17101 attgataatc agataaacag gagaatgcac gttttttaga cgttcaataa taaaatcgtc
    17161 tccccgaccg cgtttttgat cagcgctgat cataaataag accgcgtcca cttctttcaa
    17221 cgcactgtaa gctgcttcta ccataaaatc tcctaaacga tgctttggtt tatggattcc
    17281 tggtgtgtca ataaaaatca actgagcttc tggtgtagta tatactcctt ggattttatt
    17341 tctagttgtc tgcgccttat cactcatgat ggcaattttt tgacccacga ttcgattcaa
    17401 taatgtggat tttcctacat tcgggcggcc gacgattgcg acaaaccctg atttatgttc
    17461 tggcatatac gttctccttt ttaattaaac aacaaatgat atattttagg gacaaataaa
    17521 atcaacccaa cgatgacgga aaaacaagcg gtcactaaaa ctgctccagc tgccatatct
    17581 ttgatttttt tcccaatcgg atgaaaatga aaatccgtaa acatgtccac gacattttca
    17641 aaaacagtat tgatgatctc tactatccat acgagaaaaa cagctaaaag cagccataac
    17701 cattctatcc gatcaagctg gaaaatcacg cccataaaca aagcgaggat tccgaacgca
    17761 acatgtttcc tcatgttgcg ttcttcctca aaaacggttt tgactccttg gacggcaaat
    17821 tccacggaca tcaggaaatg tttgtttttc tctacgtttt tatcttttaa gtccataggc
    17881 atctaagatc tctttctgta aaccaaacat ttctttctca tcctctggtg tcatatggtc
    17941 atacccgttg atatgcaaga atccatgaac cgctaaaaag ccaagttccc gatcatacga
    18001 atggccgtaa gcttctgctt gctctgcagc acgttcagta gagatcatga tgtctcccag
    18061 tacacgaggc aatggattct ctccatcttc aaaaatcact gggatttcgt cttcgccctc
    18121 ttcttccaag gcaaaactga tgacgtctgt cggtgcatct tttccacggt aatcacgatt
    18181 gattacttgg atcgctgcat tgtccatgaa agtaacagac atttctgttt cttctggcaa
    18241 ctctaaatat gatgctgcaa attgcagcaa gtcgtcgatt tctttcattt tctcttctga
    18301 gacttggttt gtttcatcca tgaaagtaat atccattata catcgcttct ttctattctt
    18361 cttgttcttc ttttatcttc tcaacttccg atttcatttt aggatatttg atccgagaat
    18421 ggaaggtact gcttaaaccg ctgatcaatg atttttctac tttacggatt tcttttaacg
    18481 tcaatccact gtcgtccaat tgtccatcgg aaatacgctc ttcaataaga ttatggacaa
    18541 aactttcgat tttttcgctc gttggatggt ccattgctcg tactgccgct tcacaactgt
    18601 cagctatatt gacgattcct gcttcacggc tttgaggttt tggtccagga taacggaatt
    18661 gctcttccgt gacttccggg ttgcgttcct ttgccttaac gtagaaataa cgcatcagcg
    18721 tggttccatg atgctgtctg caaatatcga tcaccatttg cggcatatta taatcttcca
    18781 atattttggc accatctgtc acatgtccaa agataatatg tttactgtct tctggcaata
    18841 agaaattgtg cggattttcc gctcctgaag gcaagttctc aacgaaaaag ctggcgtgtt
    18901 tgatcttgcc gatatcatga taatagcaag ctactcgagt tagtaaagaa cgtccaccga
    18961 tttctgctac agcattagca cttagattgg ctaccatcat gctatggtga tacgtgcctg
    19021 gtgcttcttc cagcaactgt ttcaataaag gatgattagg attgctgagt tcatttaata
    19081 cgatcacact atcatcattg accatcaatt caatataggg gtgcagacct atcgtcagca
    19141 agaatgagaa cagtgcacct gtgaatccgc atactagcgt taaccaagtc gttgcatccc
    19201 caaagctcat tccttggtag ataatcaaaa tgacatccat catcacagga aaaataatga
    19261 cccacatagc agcggataac ccttgttctg agatccgttt gcgtctgacg atcgttgcca
    19321 tgagaccaga aaatacatag gacatcaaaa taactgtcag aaagttcgtg ccaatcgaat
    19381 cataaaaaat gaaaagtgcg gatactgctt gaaaaatacc agccatgatc cctgctcttc
    19441 gattcaagaa aaagcttaaa acaagtggag caaaagccgc ggggtaaaac agcggaatat
    19501 aagcagcttg ttctgtttgg aagagttgga aaaatttcat tagtacgacg cttactgaca
    19561 ttgccgtcac gtagaaaagt acgtattttg ttctttctcc tgcattttgg aattgaagtg
    19621 aattataaat cagtacaccg atttgaagca aaacagctaa aaccatggca atgagtggga
    19681 aaatagaagt cgtttgattc gtcaatccta gtaattccaa ttttttcatc gctgaagcat
    19741 caatttgatt tccttcgcga acaataattt ctccttggaa gatcatcact ggttgtacag
    19801 cttctcgtgc agattgtttt aattcttctg tctttttctc attcaataca tcattaacta
    19861 caatgccttg atccactaag tagcgaatca tttgttgctg ctctgttgtg acattcaaat
    19921 attgaatctg atcttctgct tcttgtttaa aagcttccaa ttcgctttca cgaacttgtt
    19981 tactcatctg ttcgtcaatg agcttcaaac tttcatccct aaccgtgtga atttccgttt
    20041 cagtcatttc aaatatcgtc gaataaaaat tgttcggcag tttttggtaa aacgcaacgt
    20101 tttctgcatt cacgttttca aaatttttct tcagtgtagc aatccgctct tctgtagtag
    20161 gtttcgggat attttcatct tccttcgcct tatctacctt gtcattataa gatttgttga
    20221 tggtatcatt cgtcttatcg atcaattcga ataattgctt gatccgattg ttctggtcat
    20281 ctgccagatc tttttgataa gtatattctg gagtcactgc ttctgctgcc agttttcttc
    20341 tttgttcggt ttcttccgtg ttctcaatcg ttttattcgc acggatactc tcttctgcta
    20401 cttggccttc tctgaaatca ttggcctttt gtttgacact actaaatgta acgccgcaga
    20461 caatgataaa aaacaagaac agaaggaaag gcaatgctgc ctttccgagt ttttcttgta
    20521 gtttctgcag tgttttattc aacatgtgct tatcctccta agcaaagctt attcttgatg
    20581 aagatccgcc ttttcataag ctttgatgat ttcggcaact acagggtgcc gaacaacgtc
    20641 acttgcttca aaattaacaa aatcgatttt tttgatttgc tttaatgttc gttctgcatg
    20701 aacaagacca cttgtcgtcc cacgaggcaa gtctatctga cttgtatccc cattgacaat
    20761 catttttgat tgataaccga gacgggtcaa gaacattttc atctgagcga ctgtcgtgtt
    20821 ttgtgcttcg tccaagataa cgaatgcatc atccaatgtc cgcccccgca tgtaagctaa
    20881 aggtgcgatt tcgatcacgc ctcgttccat caatcggttc gtatgatcca atccaaaaat
    20941 ttggtataaa gcatcataaa caggccgcag ataaggatcc actttttctt tcagatcccc
    21001 gggtagaaag cctaggtttt cccctgcctc cacagcgggc cgcgtcaaaa tgattttttg
    21061 cacttcgcct ttttttaggg cagcaattgc taatactacg gcaagaaacg ttttccctgt
    21121 tccggctggc cctacaccaa atacgatgtc atgtttggct accgattcaa cgtatttctt
    21181 ttgaccgctg ttttttaccc gtataggttt tccattacgg tctttgacga tctcttcctc
    21241 atacatctca acaaaataat ctaatgtacc tttttgcccc atcttcaatg ccgtcacaac
    21301 atctggcgta cttacgtgga ttcctcgttt gatcaattcc tgtaatgtcc gaatgactga
    21361 ggcagccaag gaaatctggg attgctcacc aatgatctga atgatttctc cacgtgtatg
    21421 gatggttgtt tccgttgttt cctcgatcag ttttatatgc ttgtcgtgtg cacctaaaag
    21481 catgctgaca tcatcagcag ctgtcaatct aatctctaaa gaactagact cttccgtcaa
    21541 aaatagacat cccctttttt tcgattcatt ctttcctatt ttaccatact cagaagaaac
    21601 tatatagcaa ataagaaaag gttgtaaaag agtcgtttaa ccatacgtac caagaaacgt
    21661 taaaaaatag ccgtttccat tcgctaaagg tctcgttttg ataaaaagaa gctgagaatg
    21721 taagttatat gaaagtggag aacaaaatat tccgtttcat tcagcaggta ttctctaggt
    21781 atttttcttg tttcaaaaaa agaaaacaaa atagttaaaa tattcttgca tttcaaaaaa
    21841 aacaggtgta ttattacgtc aacaaataaa catacagcaa agcgtcgagg aaaacagtag
    21901 gctattcaac gatttattcc cagtgagtca tgtaagtgag aaatgacaat aaatcgaata
    21961 gacgaacatc atttccgagt caatgtcgaa aacgtagatc attgtggctg cctccattat
    22021 caggctagcg gataacttta tcaaaaagcg tccgtgaatg aagaagcttt tttaagcgaa
    22081 aaaaggtggt accgttctag aatctagagc ccttttccca agtggaaaag ggcttttttt
    22141 atattttaag gtggtggttt tcatggaatc tgatattagt ggcattacta attgctgcaa
    22201 cgtttatatc gtcaatcaaa atgataatta ttggaggaaa aatttatgga agaaacaaaa
    22261 acttattcat ggaaacaact attgatggtc agttcactta tcttcgggat gttcttcggg
    22321 tcaggtaatc taatctttcc tgtccactta ggtcaaatgg ctggcgcaaa ctgggtcagt
    22381 gctggcgtag gatttgccat ctcgggcgct cttttcccat tactagctat cttagctgta
    22441 gtagtgacaa aaagtgatgg tctgtatgac ttagctaaac ctgtcggaaa atggtatgct
    22501 gccttgttct taatccttgt tcatttgacg atcggaccat ttttcggtac accacggaca
    22561 gctgctactg cttttgaaat ggctgctaaa ccatttttgc ctgaaaaata taattcatta
    22621 ggtatgtttt tattctctgc tcttttcttt ttacttgctt atttccttac tgtccatccc
    22681 acacggttgg tcaaatacgt cggaaagtat ctaaactcct tgtttcttgc attgttagca
    22741 atcatttttg tcgtttcttt attccaccca atgggaaatc taaatcaatc tgctgcagat
    22801 acttaccaaa caaacgcttt aacaaatggt gtcctagaag gttataacac ggttgatgca
    22861 gtagccttgc ttgccttaag tgtcactttt gtccatgcag tccgtgacct tggtttcaaa
    22921 gataaaaaag tcacagaatt aacagcaaaa gctggtagcc tgagcgttgc gctagaagtc
    22981 atgatctatt ttggtcttgt tcttctcggt gcgatgagtt tgaataagat gtctttatca
    23041 gaaaatggcg gaactgcact ttctcagatc actaattttt atttaggtaa cttcggcgct
    23101 agcttcttag gagtgatggt cacattaggt gtcttcacta ccgctatggg actcgttgtt
    23161 tcttttgcgc aagatttcca taaactgttc cctaaagtca gttatatgac atggctgcgg
    23221 ttaacgactt tcgtctcttt tgtcgttgca aatgctggtt tagacaatat catccaatgg
    23281 tctttaccag tattgatgct tttgtatcca ttgtcgcttg cgttgatctt gctttcctta
    23341 acagcaaaat ttttccaaaa gacaccattt gtctatcaag taacgatgtt gtttgctgct
    23401 gttcctgccg tattggatat gctggcaaat tcgcctgctt tagtgagtca acaaagagtt
    23461 gtagcaagca tgctcgaatt ttatcatcat cacgttcctt ttgctgcatt aggacttggc
    23521 tggatggtcc caacgttact tggttacgct ggcagcctac tgttttatta cgcctatcgc
    23581 ctatcagggt ataaacaaga agcaaatgaa ctaccagaag aataaccaaa tcaaacacaa
    23641 tttaacataa gaaatattaa acaggaaaaa accagctaga tgaaagaatc ctattctttc
    23701 gtctagctat ttttttgttc tcttttttac gcgcacaaaa aaactgcgga caatgattgt
    23761 ccgcagttta aatgataaaa ttaagattgt aatagttctt tcacaatggc atttacttgg
    23821 tttccatctg cttttccttt gactttaggc atgatcgcac ccatgacttt gccaaattct
    23881 ttcattgatg aagcaccagt ttgtgtgatt gcttcttgaa caagttgtcg aatttcttcg
    23941 tcggaaagtt gttccggcat gtatttttcg actatcacaa tttcactttt tactttttca
    24001 gctaaatcgt cacgtcctgc ttcttcaaac tcatgcaaag aatcacgtcg ctgtttcatt
    24061 tcgcgagaca acaccgttaa ttcttcttct tctgtcaagt cgtgaccttc tttaatctgc
    24121 tcgttttgta tagatgactt gatcattcgg atgacttgta aagattcctt atctttcgct
    24181 ttcatcgctg ttttcatgtc atcgtttaat ctacttagta gtgacacact accaactcca
    24241 attcatcaca gaactagaat tttttgcgtt ttctagctgc ttcagatttt ttcttgcgtc
    24301 ttacacttgg tttttcgtag aattcacgtt tacgagattc ttgtaaagta cccgcttttg
    24361 acacggaacg tttgaagcga cgaagagcat cgtcaagtga ttcgtttttg cgaacgactg
    24421 tttttgacat gttaattccc tccctccgag cttaaaaagt aaccgtaatt gttattgaaa
    24481 tatatctcaa aaaacaagac tgtcctgtaa atattatatc taatgctgtt ttgaccgtca
    24541 aggaaatctt gctttttttt aaaattatca aatgttattt tatttaaatc cttattgaca
    24601 attttcacat cttccgaaaa tctcgaatct atgcccttcg atcgtacatc caccgagctg
    24661 ttcttcgaaa taattcattg gacacatatg gatttctttc gttgttccgc acaccgtaca
    24721 gataaagtga tggtgatggc cgatttcccc ttgacagcaa tgaaaacgaa acttcatctc
    24781 tccatttaaa tcggtttctt ccagtaaatc gatttctgca aaatcatgga gatttcgata
    24841 aatcgtatcg taacttactc ctttgaattc ctgattcata tagtcaaaaa cttctttagc
    24901 ggatacgtag cgattgcgct tcactaaata agttaacaat gcttctcttt ttttagtata
    24961 tttaaaaccg ttatttttca atgtttctag tgattgttca atcaatgtct gttttttcat
    25021 gacttattcc tccaaatcaa aatgattacg aagtacacat tctagagtat ggtataataa
    25081 acataaaaaa aatgcaagtg aggttttatc catgtcttcc gattctacag aaatcgttac
    25141 tttttttgtc atctctgact ctgctggaga aactgcgaca aaattagcgc aagccactat
    25201 ggcccaatat cctaccgttg agttcaattt attccgcaga acatttgtga cagatgagaa
    25261 aacattgatg caggcactgc aagatgcact aaaagaaaaa gcccttgtcc tgcacacatt
    25321 gatcaatcaa gaattgatcg agatgacgcg ttctttttgt gaagaacatc aattatttag
    25381 ttttgatgtg atgactccgc cgatatcaga aatcgagcgc ttaactggta tcaagcccat
    25441 tcggcaacct ggcgcccttc atttattgaa tgagaactat ttcaaacgga tcaaagcaat
    25501 ggaatttgct gtaaaatacg atgacgggaa agatcctcgt gggtttctag aagcagatgt
    25561 cgttttgcta ggcgtatcca gaacttccaa aacgcctttg agcttgtttt tagctaacaa
    25621 aaatttaaag gttgccaatc tacctttgat cccacaagca catattccca aacagctttg
    25681 ggagatcgat cctaaaaaaa tcgtgggact gacgaataac ccagatattt taaacaatat
    25741 ccgtaaagaa cggatgcgct cttacggatt aaatcctgat actgcttatt ccgatatcga
    25801 aaaaatacga gctgaactcg attttgcgaa tgatctttat gaaaaattgg gctgcgtagt
    25861 aatcaatgtc gcttctttat ctatcgaaga aacagcttca cttattttga atgctttaga
    25921 cctagaagat catagttatt atggaactga aaccaccgaa gaaacagact aacagttttt
    25981 cttgtctttt tcttaaaaaa actggactgt gtcagcgaag aagattcgtt gatacagtcc
    26041 agtttgtctc tatttgcacc tatcgttatt gatgttccac atgtttgagc gtctggttca
    26101 aaatatccag tacgtgagaa tcatcaagac tatacaaaat cgcttttccc tcacgatggg
    26161 acttgacgac atggttttct cttaataaac gcagttgatg cgatacagca gattgttcca
    26221 tttctactgc ttgggtgatt gctgtcacat tttgttcccc gtctttcaga tacagcagaa
    26281 ttttgagacg agtggggtca ctcaacattt tgaatagttg gctgactttc tctacttttg
    26341 ttttttgcgt gtctggcaat ccattcacct ccaacttgaa aaaagactga cttctaaagt
    26401 ttaccacttt ttccatcagt ctcaagtttt tttattcttc tgtcattttt ttgataatcg
    26461 cttcataagc ggctactttt tcagctgcgt cttctgaaga gtttcctttt acaccgatat
    26521 aaaatttgat tttcggctca gttcctgaag gacggatagc gatccagcca ttttcttcca
    26581 gcgtatactt caatacgtca gatggcggtg tcgttagttt ttctacgttt ccttcacggt
    26641 ctgttctcgt caactctttg aagtcttctg tttggatgac ttttgctccg gcaaattctg
    26701 tcggtgcttc ttcacggaat ttttccatca aagcagagat ttttgcagaa ccttcttgtc
    26761 cgctcattgt cacagaaatc gttttttctg cgtagtagcc atatgtttca aaaatcgttt
    26821 gcaagccgtc atacaacgtt ttgccttgtt ttttatagta agctgctact tctgctaata
    26881 agagcaatgc ttgaatcgcg tctttgtcgc gaacgaaagg tttaactaaa taaccgtaac
    26941 tttcttcgaa tccgaacata aaagtatggg aatgatcttc ttcaaattgt tgaatttttt
    27001 cagcgatgaa tttgaatcct gtcaatacat tgaacatggt agccccatag tgttccgcaa
    27061 ttgctgtagg caattcgcta gaaacgattg atttcaatac cgctgcattt tcaggtaaag
    27121 tgcctcgcga ttggtgtgct tccaaaatat attgagcaaa cagggctccg atttggttcc
    27181 ctgtcaatac ttcataggag ccatttggca aacgaacggc tgcgcctaaa cggtctgcat
    27241 caggatctgt tgcaatcagt aagtcagcgc cttctttctc gccaagtcgc atcgcatact
    27301 cgaaagcaga gtgttcttct ggattcggtg atttgactgt agtgaaatcc gggtctgcga
    27361 cagcttgttc tggaactagt acgaattgtt cgaatccagc ttgtttcaac gcacgttcgc
    27421 ccaacatttt tcctgttccg tgaagtggtg tgtacacaag tttcaaatct ttacccattg
    27481 tttccaccaa ttcatgatca atcgttactg ctttgatttc ttctagataa gcggcatcca
    27541 cttcttctcc gatgatcgtg atcaatccgc tatgttcagc ttcgtcgtca gataatactt
    27601 caacttttaa cggattgctg acttcacgga catatttcgt caatgcatct gcgtctgcag
    27661 gaggcatttg tcccccatct tctccgtaga ctttatatcc gttgtaagct gctggattgt
    27721 gggatgcagt taccatgatt cccgcaaacg tcttcaaatg acggactgca aaagaaagct
    27781 ctggtgttgg acgtaaactt tcgaatacat aagaagggat attgtgatgt gctaatgttt
    27841 tggctgcttc catggcaaat tctggtgatt gatggcgtga gtcataagcg atagcgacac
    27901 ctcggcgttt tgtttcttca ccttgtgtat ccatgaaacg tgcaagtcct tctgttgctt
    27961 gtcttaccgt atagatgttc attcgattga tcccaggtcc taagattccc cgcatgcctg
    28021 cagtaccgaa ttccaacggt gcataaaaag catcttcaag tttttcagga tcttcaccca
    28081 gatcaattaa ttgttttttt aagttttctt ctaaattttc ttcatgtacc cattgttcat
    28141 acacttgttc ccaagacatg aaaataacac ctctttctga aatatggttc ttaataagta
    28201 tagcattcac ttattagata tgaaagtacg aggtctaaaa aacgcttaaa tcaagaaagg
    28261 attcttttat caagcacggt tgcaagtatt cgcataaatc ggattcagta gatttcacgc
    28321 gaaaaaaaag agtaaaagaa caattgaaac cgttcttcca ctctttttaa acttatgcag
    28381 ttgtttcttc taaagattcc aagtatttat acacttgttg tccagcgatc ccaccatcac
    28441 caacagcagt agtgatttgt cgcaaatctt tttcacgaac atctccgatg gcaaaaacgc
    28501 ctggtatatt cgtcttcatt tcgttgtctg ttggaatcca gccagcttcg ttcgtcaacc
    28561 ctgcttctct aaatggtgca gttaatggat ccagtccaac atagatgaat actccgtctg
    28621 ctgccaaatc aaactcattg ccggttttga cattacgtgc tttcacacct gtcactacca
    28681 tttcattccc aacgatttcg tcaacgactg tatcccacat aaaggaaatt ttttcattcg
    28741 caaatgcacg gtcttggatg atcttttgtg cacgtaattc atctctacgg tggacgatga
    28801 ctacttcaga agcaaattgt gtcaaataga tggcttcttc tactgctgag tctcctcctc
    28861 cgatcactac tagacgtttg ttacggaaaa acgcaccatc acaaactgca cagtaagaaa
    28921 cgccacgtcc agcaaattct tcctcgccag gtacgcctaa tttacggtgg atacagcctg
    28981 ttgcgataat gactgttttt ccttcgtaag ttttatcttc acaaataact tctttgtggc
    29041 tgccgtgatc cttgatatct tgcacgattc cataagcatt ttctgtccca aatcttgaaa
    29101 caccttcata catcttataa gctagatcag gacccatgat tgaatcgaaa ccaggatagt
    29161 tttcgatttc tgccgtattg ttcatttggc cgccaggggc gccttgttcg atcatcaata
    29221 cggataagtt tgaacgtgaa gcatataaag cagcagtcat cccagcagga cctgcgccaa
    29281 taacgattac atcatacata tgtagattct cctttactta atcaagtaag catactttac
    29341 agccaatgcg aaaaaaagtc tattgttctg cttgcgtttc tcgaatagct ttattatact
    29401 cgaaaaatcc gctgaaaaac aatagacttt tacaaatcat tgatcaatca ttcgaaagat
    29461 tttctccatt tgaatcaatt acttttttat accagttgaa tgatttcttc ttcgaacggg
    29521 ctaatgtgcc atttccttcg ttgtcacgat cgacataaat gaatccgtac cttttcttca
    29581 tctcacctgt accagctgat actaaatcaa tacatcccca agtcgtatag cctaatagat
    29641 cgactccatc taattctact gtatctttca ttgcctggat atgtgctgcc agatagtcga
    29701 ttcgatagtc atcttccaca taaccattct catcgggcgt atcgactgca cctaagccgt
    29761 tttctacaat aaatagtggt ttttgataac gatcataaat atcattcatc gttattctca
    29821 agcctaatgg atcaatttgc caaccccatt cacttgcttc cagatatgga tttttgacag
    29881 aagcaaagat atttcctgca gtctgttcca agagttcgtt gtcagtagtc gaaactcgag
    29941 aagaataata agaaaaggag ataaaatcaa cagtatgttc tttcagcaat gcggcatccc
    30001 caggttccat tttgatctga atgccatttc gttccatttc tttcaatgca taagcgggat
    30061 attctcctcg tgactggaca tcaatgaaga aataattttc tcgatctgat tttctagaag
    30121 cccaaacatc ttcaggattg caagtgtatg gataatatgc tcctgctgcc agcatacagc
    30181 ctactttgtt ctccggatct acttcatgag caatcttcgt tgcgatggca cttgcaacca
    30241 actcatgatg tgccgcttga tatttgattt gttcttcatt ttctccttct tcaaaataca
    30301 gccctgcgcc catgaatggt gcatgaagga tcatattgat ctcattgaat gtcaaccaat
    30361 atttgacaag tcctttatag cgattgaaga tgactcggca cagattttca taaaaaccaa
    30421 cgagttctct agaacgccac gcaccatatt ctttgatcaa atgcatcgga caatcaaaat
    30481 gtgtaatcgt cacaagcggc tcgatcccgt acttttgaca ttctttaaag acatcctcat
    30541 aaaatttcaa accagattca tttggctcat tttcatcgcc cattggaaaa atacgactcc
    30601 aagcaatgga catccgataa gtcttgaacc ccatttctgc aaacaaagca atatcttcct
    30661 tataatgatg atacatatcg atcgcaattt ttgctggata aaaatgatct ttatcaaatt
    30721 caaacatttt cttttttcca gtaatgacag gaaaacggtc ttctccgatt ggcacaacat
    30781 ctacgttcgc taacccacgt ccgccttcat cataaccacc ctcgcattga ttggctgctg
    30841 tagcaccgcc ccataaaaaa tcttttctga atcccatcat acttcctcca ttacatgtgt
    30901 attgcttgac aattctcttg ccagttcatc ttggacttcg attccttctg cttttaaccg
    30961 gctgattgtt tccttgaatt gcggtaaata ctgctgatgt gcaataaaca actcatccat
    31021 gatccgtttt gctgtctttc ctgatcgtat aagcggattg atcgtaaacg cttgtaaagc
    31081 tgttccataa tcaccagtca cggctgcttc gatcgtcagc aattccatat tcttcattac
    31141 ttgcaaccag cctctttcag cagcaggaag ggaaccaaac gctacatttc gtgcgccttg
    31201 tgcgccaaca taggcagtga cttctactac acaatctgcc ggcagatcag gaacagctcc
    31261 atcattcttt gtagacacca cgatctgtgt ttctttattt ccatagatcg aagcaatcgt
    31321 ttcacaggca gcatctgaat aataagcacc acctcgtttt tgcaattgtt caggtttatg
    31381 atccaattct ggatctttgt ataattcaaa cagctctgct tctgtttctt tcacttgttg
    31441 cgctcttgta ccaatcgttt gatattcttc taaagcatgt tccaacattt cttcttcacg
    31501 gtagtaatag cggtggtagc cacaagggat catgttcatt tgttttaatt gttctttgaa
    31561 aaacgggatg tcaaagatat ttttcggcaa tccattgtct tcatcgtata aacgatcaat
    31621 caaatctgct gtgcggtcat ttcctttatt atctgctact ttgtgccaat ggaagtgatt
    31681 tagaccggca aacttgtaga tcagctcttc ttttttcttg ccaagcattg ctggttctgt
    31741 catcgtcgcc attactggca cattgcataa tccgatcact ttatcccatt tgccatatct
    31801 gataactgct tctgtcacca tcccgcttgg gttcgcgaaa ttgatcagcc aagcatttgg
    31861 acataaacgc ttcatgtctt caacgattgc caaaaccact ggaacggtac gaaaagcttt
    31921 gaatatcccg ccagcaccat ttgtttcttg cccaatcatc ccataataac tagggatccg
    31981 ctcgtctttg attcgtgcat ctaattgacc gacacgaaac tgagtcgtaa caaaatccgc
    32041 atctttcaat gcttcttccc gatccaaagt tgcatagatc ttgacttcat acggagaagc
    32101 atcccacata cgctgagaca tctgactgac gatctcaagc ttttcttttc cttcttctac
    32161 atctacgagc cagatttctc gtatcggtaa ttcctcatat cttttgataa atccttccat
    32221 caactcgggg gtgtaactgc tgcctccacc aatcgtagcg attttgattc cttttgccat
    32281 gttctcactc ctttgctttt tacactttta ttatcttttt tgtaaacaaa acaaacaata
    32341 gaaattcgtt tctttccagt aacatttaca aaagttagtg ttttctactg gatttttgta
    32401 aatatcttgt
//
LOCUS       DAIGOK010000016        32183 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.016, whole genome shotgun sequence.
ACCESSION   DAIGOK010000016 DAIGOK010000000
VERSION     DAIGOK010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.016"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            72..440
                     /locus_tag="NOA01_000573"
     CDS             72..440
                     /locus_tag="NOA01_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HCI0631797.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            610..1041
                     /gene="mraZ"
                     /locus_tag="NOA01_000574"
     CDS             610..1041
                     /gene="mraZ"
                     /locus_tag="NOA01_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCI0631798.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            1062..2021
                     /gene="rsmH"
                     /locus_tag="NOA01_000575"
     CDS             1062..2021
                     /gene="rsmH"
                     /locus_tag="NOA01_000575"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCI0631799.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            2026..2427
                     /gene="ftsL"
                     /locus_tag="NOA01_000576"
     CDS             2026..2427
                     /gene="ftsL"
                     /locus_tag="NOA01_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCI0631800.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            2428..4620
                     /locus_tag="NOA01_000577"
     CDS             2428..4620
                     /locus_tag="NOA01_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCI0631801.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGIAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            4646..5608
                     /locus_tag="NOA01_000578"
     CDS             4646..5608
                     /locus_tag="NOA01_000578"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCI0631802.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            5622..6983
                     /locus_tag="NOA01_000579"
     CDS             5622..6983
                     /locus_tag="NOA01_000579"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCI0631803.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            7009..8097
                     /gene="murG"
                     /locus_tag="NOA01_000580"
     CDS             7009..8097
                     /gene="murG"
                     /locus_tag="NOA01_000580"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCI0631804.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            8112..9332
                     /locus_tag="NOA01_000581"
     CDS             8112..9332
                     /locus_tag="NOA01_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HCI0631805.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            9488..10813
                     /gene="ftsA"
                     /locus_tag="NOA01_000582"
     CDS             9488..10813
                     /gene="ftsA"
                     /locus_tag="NOA01_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCI0631806.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            10836..12077
                     /gene="ftsZ"
                     /locus_tag="NOA01_000583"
     CDS             10836..12077
                     /gene="ftsZ"
                     /locus_tag="NOA01_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCI0631807.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            12094..12771
                     /locus_tag="NOA01_000584"
     CDS             12094..12771
                     /locus_tag="NOA01_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HCI0631808.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            12785..13384
                     /locus_tag="NOA01_000585"
     CDS             12785..13384
                     /locus_tag="NOA01_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HCI0631809.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            13399..13686
                     /locus_tag="NOA01_000586"
     CDS             13399..13686
                     /locus_tag="NOA01_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HCI0631810.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            13735..14517
                     /locus_tag="NOA01_000587"
     CDS             13735..14517
                     /locus_tag="NOA01_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCI0631811.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            14538..15245
                     /locus_tag="NOA01_000588"
     CDS             14538..15245
                     /locus_tag="NOA01_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HCI0631812.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            15507..18305
                     /gene="ileS"
                     /locus_tag="NOA01_000589"
     CDS             15507..18305
                     /gene="ileS"
                     /locus_tag="NOA01_000589"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCI0631813.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(18354..19880)
                     /locus_tag="NOA01_000590"
     CDS             complement(18354..19880)
                     /locus_tag="NOA01_000590"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCI0631814.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(19895..20542)
                     /locus_tag="NOA01_000591"
     CDS             complement(19895..20542)
                     /locus_tag="NOA01_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HCI0631815.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(20726..21055)
                     /locus_tag="NOA01_000592"
     CDS             complement(20726..21055)
                     /locus_tag="NOA01_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HCI0631816.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            21232..21960
                     /gene="sfsA"
                     /locus_tag="NOA01_000593"
     CDS             21232..21960
                     /gene="sfsA"
                     /locus_tag="NOA01_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCI0631817.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALAEMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            21976..22989
                     /locus_tag="NOA01_000594"
     CDS             21976..22989
                     /locus_tag="NOA01_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCI0631818.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            22989..24266
                     /locus_tag="NOA01_000595"
     CDS             22989..24266
                     /locus_tag="NOA01_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCI0631819.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            24329..27031
                     /locus_tag="NOA01_000596"
     CDS             24329..27031
                     /locus_tag="NOA01_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCI0631820.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            27183..27500
                     /locus_tag="NOA01_000597"
     CDS             27183..27500
                     /locus_tag="NOA01_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCI0631821.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            27530..27850
                     /locus_tag="NOA01_000598"
     CDS             27530..27850
                     /locus_tag="NOA01_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCI0631822.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            27958..29418
                     /locus_tag="NOA01_000599"
     CDS             27958..29418
                     /locus_tag="NOA01_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCI0631823.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            29486..29707
                     /locus_tag="NOA01_000600"
     CDS             29486..29707
                     /locus_tag="NOA01_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631824.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            29738..29920
                     /locus_tag="NOA01_000601"
     CDS             29738..29920
                     /locus_tag="NOA01_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HCI0631825.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            29920..30333
                     /locus_tag="NOA01_000602"
     CDS             29920..30333
                     /locus_tag="NOA01_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HCI0631826.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            30456..31637
                     /locus_tag="NOA01_000603"
     CDS             30456..31637
                     /locus_tag="NOA01_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCI0631827.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(31708..31872)
                     /locus_tag="NOA01_000604"
     CDS             complement(31708..31872)
                     /locus_tag="NOA01_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631828.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            32026..32099
                     /locus_tag="NOA01_000605"
     tRNA            32026..32099
                     /locus_tag="NOA01_000605"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32060..32062,aa:Arg,seq:cct)
BASE COUNT        10776 a         5517 c         6854 g         9036 t
ORIGIN      
        1 aataaaatga aacagttgtg taaagtatat acagtatact tgaagcaacg ataaaaaagg
       61 agtttacgtt catgggcaca ttcacaccac ttatcttgtt ttggtatatt tacccggtaa
      121 tcgttttgtt tggttgccaa tttcttgtaa aattattttc gttaaatcgt cgttttaaaa
      181 taaaagcccc tgatttggcg attccttttt tgtggggcgg tatgcatgca ttgtctagaa
      241 atacagggac aatttctatc ttaccgtttt tctttatttc tgttttgcta atgggaattt
      301 gtattgctgt tttccaagca tattattatg aagaaattat ttatccgcgt tatttcaaga
      361 tgacttggcg tttggttttt ctgttgacaa tcgtcttgta cgctgtcttg atcattgtga
      421 acatactttc ttacctatga aaaaaataga aaagctgtaa aaagtctttt aaaaggcttt
      481 ttgcagcttg atttttaaaa aaatgtttat gaataaaaaa agttgttccc ggatttccca
      541 gaaaaggtgg tagaaagtgg gggattgtgg tagactaatt ttagttagtg gaggaatggg
      601 gggctttcaa tgttgatggg tgaattccaa cataatattg atgcaaaagg acgtttaatc
      661 gtgccctcaa aacttcgtga agaacttggc gaaaagtttg ttctgacacg cggattggat
      721 ggctgtcttt tcggctatcc gatgagcgaa tgggaaaatc tggaagctaa actgaacgaa
      781 atgccgttgg ctaaaaaaga cgcacgtact tttgtgcgat tcttctattc ggctgccact
      841 gaatgcgaac ttgataaaca aggacgaatc aatataccca gcacgttaag aaattatgca
      901 gctttgacaa aaggttgcgt agtcatcggt gtatctaacc gaattgaaat ttgggatgag
      961 acacgatggc aagatttttc tgcagcagca gaagagaact ttgacgaaat tgctgaaagt
     1021 atgattgatt tcggactata agaataggaa gaggctgact aatgactgaa acatttcaac
     1081 actatacagt catgttgaaa gaaacggtag atggattgaa catcaaaccg gatggcgttt
     1141 atgtagactg tacactcggc ggtgccggac atagtgaata tttattaagc caactaagtt
     1201 ctaaagggca tctgtatgcg tttgaccaag atcaaaaagc aatcgatcac gccaagctgc
     1261 gtttagcttc ttatgtcgaa caaggtatgg tcacatttat caaagcgaac ttcagagaat
     1321 tagaagaacg cttgagtgag catgtagata aggtagacgg cattttgtat gatctagggg
     1381 tttcctctcc tcagctagat gaagcagaac gtggatttag ttatcatcaa gatgcaccgt
     1441 tggatatgcg catggatcag tcagctcctt tatccgctta tcacgtaatt aatgaatatt
     1501 cctaccatga attagtaaag attttcttcc gctatggcga ggagaaattt tccaaacaaa
     1561 ttgctcgtga aatcgaacga gtacgaaaaa caaaaccaat ccaaacaact ggtgagcttg
     1621 tcgaaatcat taaacaagtc atcccagctc ccgcaagaag aaaaggcggg catcctgcaa
     1681 aaagaatttt tcaagctgtg cggattgcag taaatgacga actcggtgcc gaagaggcct
     1741 ctttagaaca ggcaatccgg ttattgaaag tcaatggaag aatcagcgta atcacattcc
     1801 attcattgga agacagaatt gtcaaaagta tgtttaaaga atatagtacg gtacaagatc
     1861 taccaccggg acttcctgtc atacctgacg aattccaacc agaactgaaa gtcattactc
     1921 gtaaaccaat ccttccttca gaggaagagc taacagaaaa caatcgatca cgcagtgcaa
     1981 agttacgtat tgcagaaaaa ataagaatga aagaggagta gaatgatggc agaattaaag
     2041 aaaatgcaag attacgaata tgatttacct gaattggtgg atcagccgga acaacaaatg
     2101 cctgattctt cttcgaatca agaaatcatt cttccacaat caccggcaaa acgattaaag
     2161 cagatttctg ctttggaaaa aattgctgtg gcaagcgtgg cgattgctat tgttgcttta
     2221 tgtgttttaa cagttatgct gcgcacaaat atcagcggag ctgaaaaaga aatcacgtca
     2281 atacaaacag aaatcaatga taaaaagcaa gaaaaaacga gtttagaaca agaaaaaaac
     2341 gaactttctc gtactgaacg gattaaacaa atcgcagaag aaaaaggatt gtctattaat
     2401 gacgacaatt taaggaaagt gaagtaaatg agtcttaaaa ataaaattcg gcggtttatg
     2461 gaaaagaaaa atctaaatcc gatgaacaat cgcaaaaaag taggtatcat tttatttgct
     2521 acgagtattg gattgttctt tttatttgcc gtccgtttct cgtatatcgt tattggaggt
     2581 catgttgctg gtacctcatt agctgaaaaa acaaaacagt tgtaccaagg aagtgaagta
     2641 gtaaaagcaa aaagaggaac gatttatgat cgtaacggtg tggcgctcgc agaagatgcc
     2701 tcctcttatt caattaaagc gatactctca aaaacatata cctctgggga taaaaaactg
     2761 tatgttgaag aaaagaattt tgataaaata gcagaaatcc tgcataagaa tctttctata
     2821 gacaaaaaag atgcactgaa tattttagag gacggcgcaa aaaaagagct gtatcaagtt
     2881 gaatttggta gctatgggaa aaatatctcc caagaaacca agcaaaacat cgaagcggac
     2941 atgaaaaaag aaggtgtcgc tggtctttat tttgtcgacc atcaggctcg gatgtatcca
     3001 aatggcgtct tttcttctca ttttattgga tacgcagttc cagacaaaga tgagaatggc
     3061 ttagttggta aactaggttt ggagtctgct tacaatgata tcttgagcgg taaagatggt
     3121 aagattattt atcaaaaaga taattttcaa aatcctctgc caggtacagt ggcagaagaa
     3181 gaaaaagctg tcgacggaca agatatctac acgactttag acagcagact acaaagttat
     3241 ttagaaacac tgatggatca agtaaatgaa gaatatcagc cagaagaatt gacagcagtc
     3301 ctgatgaaag caaaaaccgg agaaatcttg gcaatgggac aacgacctac tttcaatcct
     3361 gaaacaatgg aaggattgac tggaaaagat gctatctggc gtaatttctt ggtacaagat
     3421 agttatgaac caggatcaac catgaaagtt ttcactactg cagctgctat cgaagaagga
     3481 gaattcaacg agaatgaaac cttccaatct ggaaaaatcc aagtagaaga tgctacgatc
     3541 aatgaccatg actttggtga aaaaggtgtg ctgaccatgc gtcaagctct ttcatggtca
     3601 agtaacgtcg ggatggtcat actggaacag cgattaggcg gacgatggta taactattta
     3661 caaaaactgg gatttggaca aagtactcat tctggattag atgatgaagt aaacggtgct
     3721 ttacctacat taaatattgt cgaccgagca atgagtgctt atggacaagc ggtaggtgtg
     3781 acgaatttcc aaatgatgaa agctttcact tctattgcga ataacggtac aatgattcaa
     3841 cctcgatata tcagcaaagt ggtggatccc caaactggag aagagcgaac aactcaaaca
     3901 gaagttctag ggcaaccttt ttcaaaagaa acaacagaaa aagtccgtga atatatgaga
     3961 gatgttgtcg aaagcgaaaa ctacggtatt gcttatggtg tatatagtgt acctggatac
     4021 aatgtatcgg caaaaaccgg aacagcccaa atcgcttctg atacaggggg atatcaaaca
     4081 ggagatacag cctacctgta ttcgattgtg gagatggttc cttctgaaga tccagactat
     4141 gtcttgtatc taacaatgaa acaccctaag acatatgatc gaatggcatt ggctaaaatc
     4201 gcaaatccat tgatgaaacg ggcaatggat tttaaagaaa ctgaagaaga ttcagataca
     4261 gaaacaaaaa cagaaaaagt atccgtagcc gattatcgta atcttgaagc ggacgttgca
     4321 gcagctgatg ctcaaaaaag tggactgcaa cctgtagtga tcggtaatgg taaaaaagta
     4381 caaaaacaat ctactgccaa cggtgatcag ttgatctctg gagaaaaact gatcctatat
     4441 actggtggtg ataagctgat gcctgacgta acaggatggt caaaagcaga tatcatgaag
     4501 ctggggaaaa tcctcggtat tgaagttagt tttgatggag atggttattg tgtcaaacaa
     4561 gaacttgctc catatgaaaa aatcactgaa gacaaactta gttttacttt agaagaataa
     4621 gaagagaaat aggagagaaa agagcatgga atggacacaa gcattgattc ccattgtcag
     4681 cagttgtgca atgacaattg ccgcaatgcc gctatttatt ggttattttc aaatgaaaaa
     4741 gcaaggtcag gcgattcgtg aggagggacc aaagtggcac aatagcaaag ccggaactcc
     4801 tacaatgggc ggcttagtgt ttctgatcgg ctctattttg acaggaattt gggttggagc
     4861 ttggcaaaaa cagctaactc ctactctttt catcttgctt tttgtattag cgttatacgg
     4921 agtaatcggc tttcttgatg attttataaa aatattcaag aaaagaaaca tgggactgaa
     4981 ctcgaagcag aaattgattg gacagattct agggggaatc atcttttatc ttgtttaccg
     5041 ttctgaaggt tatccaggtg tattgaattt cttcggacta gatctacctt taggaatcat
     5101 ttatggtatt ttcgctctct tctggcttgt tggattttca aatgcagtaa atctgacaga
     5161 tggcatcgat ggattagttg ctggcttagg aagtatctct tttgcagctt acggtctaat
     5221 tgcttggcat cagcatcagt atgatgtatt aatcatttgc ttaagtgttt taggaggttt
     5281 actgggtttc ttcgtctaca acagaaaacc tgccaagatt tttatgggag atgtcggttc
     5341 attagcgttg ggcggacttt tagcggcaat ttctataatg ctaaaccaag aatggacatt
     5401 gcttttgatt ggtttgatct atgttatgga aacagcaagt gtgatgcttc aagttacttc
     5461 ctttaagctt acaggaaaac gaatttttaa aatgtctcca attcatcatc actttgaaat
     5521 gtgcggatgg tcggagtgga agattgatac agttttttgg ttgatcagca ttgtgacttc
     5581 gttgatcaca ttatggatcg tttggtagga gatgacggga aatgaaaaaa atatcaacat
     5641 atgaaaataa gaaagtgctt gtcttaggac tcgcaaaaag cggagtaagt gcggccaaat
     5701 tattacatga attgggcgca cttgtcaccg ttaatgatgg aaaacctttt gacgaaaatc
     5761 cggaagcaca agagctgctt tctttaggga taaaagtaat aacaggtagt catccaatcg
     5821 aattgttgga tgaagaattt tcattgatgg tcaaaaatcc agggattccc tacagtcatc
     5881 ctttagtagc aaaagcccaa gaaatgggta ttccggtaat tacggaagtt gagctagctt
     5941 acgaagtagc ggaatgccca attattggga ttacaggaac gaacggaaaa acaacgacaa
     6001 caacgatgac aggactacta ctgaatgcag gagcagatca aggaattgca cgtttagcgg
     6061 gtaatattgg gtatccagca agtggtgtag cgcaagaagc aaaatcagaa gataaaattg
     6121 tgatggaatt gtctagcttc caactcatgg gaatcacaga tttccgccct catatagcgg
     6181 tgattaccaa tatctatgaa gcacatattg attatcatgg tacaagaaaa gaatatgtca
     6241 aagctaaatg gaaccttcaa aaaaacatga cagaaaaaga ttatctgatc cttaactgga
     6301 atcagtcaga actacaagaa ttagcgcaaa gaacaaaagc acgcgtactg cctttctcga
     6361 caaaagaagt gttggaagac ggtgtgtatg ctgacgatta ctcaatatac tataaaaaag
     6421 aaaaaataat ggaaatatct gaacttggcg taccaggtaa gcataatgta gaaaatgcac
     6481 tagctgctat ttctgtagca aaattgtatg gtatatcaaa tgaagctata cgagaaacgt
     6541 tgcatttttt ccatggcgta ccacatcgta cgcaatatgt aggagaaatc caaggcagaa
     6601 aattttataa tgattcaaaa gcaaccaata ttttagcgac taaaatggct ctaagtggat
     6661 ttgaaacgtc taaggtcgtg ttattggctg gcggattaga cagaggaaat acatttgatg
     6721 aattaattcc ttcattaaaa ggaatcaaag caatggtcgt atttggccaa acaaaagaaa
     6781 agttgatgga tgctggaaaa aaagcaggca tcgagacaat agtaacagcc gattctgtag
     6841 aacaagcagt tcctttagca ttagaaaaca gtacagatgg tgatgtagtt ttattatcac
     6901 cagcgaatgc cagctgggat caatacccaa attttgaaac tagaggaaac agattcatgg
     6961 aagctgtgaa ccgattaaaa tagaaaaagt agaagcgagc gataaaaaat gaaaattttg
     7021 gttacaggcg gaggaacagg gggacatatt tatccagctc ttgcctttgt caattatgta
     7081 aagactcaag aaccgaatgc tgaattcatg tatgtgggag caaaacgagg tctagaaaac
     7141 aaaatcgtcc ctgatacagg aatgcctttc cataccttgg agattcaagg atttaaacga
     7201 aaaatttcca tgcataacat caagacggtc cgattgtttc ttaagagtat ccgtcaagca
     7261 aaaaaaatcc tcagtgattt tcaaccagat atcgttatcg gtactggagg atatgtttcc
     7321 ggagcggtag tgtacgctgc agccaaaatg ggtattccaa cagttatcca tgaacaaaac
     7381 agtgtaccag gtgttaccaa taaattttta agccgatatg tggatcggat cgctctttca
     7441 tttaaagatg cggcttcctt ttttcctgaa aacaaagcag ttcttgtagg taatcctcga
     7501 gcacaagaag tagcagatac taaaaaatca gatattttga aaacatttga tttggatcca
     7561 gaaaagaaaa cagtcttgat ttttggcggt agtcaaggag cacttaaaat caatcaagca
     7621 gtatcagttt ttttaagtga atatattcct tcttcttacc aagttttgta tgcttcagga
     7681 gatagatatt atgaagagat cctaaataaa ataaatcctg aaatacaaaa tattagtgtc
     7741 cagccttata tcaaaaatat ggcagaggtc atggcttcca gtgatctgct ggtggggcgt
     7801 gctggtgcaa cttcgatcgc agaattcaca gctctggggt taccggcagt gttggttccc
     7861 agcccatatg ttacgaatga tcatcagact aaaaatgcaa tgagtcttgt tcacgcagga
     7921 gcagcaaaaa tgattgcaga taatgaacta acaggtgaat cactaagtca gacagtcaac
     7981 gaaatcatgg gtgatgaaga attgcaaaag caaatgtgcc gagcttctaa agaacaagga
     8041 atccctgatg cttcaaaacg tctttatgac ttagtaaaac aaataatcaa aaaataaagg
     8101 cggtggtctt agtgagtaag aaaaaagaac caaaaaaatt gtctcatccg tcttctgatg
     8161 aaaacaattt gactccttgg caaaaggcta accgaaaata tctggcagaa caagagatga
     8221 aaaaccaaga gccaaaaaaa gaagacataa cattagatac acccctttca gattctgaag
     8281 aagaaaaaac ggttcaagag gaacagcaag aagaaagcca gtccgagaaa aaaatcgaat
     8341 tttttgaaga gatcaagcaa aagaaaaacg gtggaccata taacggttct tttttgaacc
     8401 ggctgcccaa tttaaaaagt caacggcaca aagtactatt tcgtcggtta gctttgatca
     8461 tcactgtatt gggtataccg ctgatttttt tgatctatta tgtttctcct tatagcaaat
     8521 tacaagcagt tgcagtatca ggcaataaga cagtcaacag ccaagaaatc atctctgata
     8581 caaaattgtc attaggagaa aatgtttggg gacagtattt tcatcgatct acttatatcg
     8641 atcgccttaa aaaagcacag ccaagaatcg aaacagcaaa tatacatttc aaagggatga
     8701 atgaattcga tttggatgta acagaatata aagagattgc tttgatcgca aaaaacaatc
     8761 aatactaccc agtgattgaa aatggaacag tattagatga aaaagtagca aatcctacaa
     8821 aaaatctccc tatcttggaa gagttcaaag actctgcaaa aatcaaagaa ttaacaaagc
     8881 aatacaatca gttatcatca gaattacaaa aagcaatttc tgaaatcaag tacacgccaa
     8941 gagcaagcaa taaaaatctg atccaattga atatgaatga cggcaatcaa gttattgtaa
     9001 atatcaataa tcttgcgaac caaatgaaat attattcgca agtagccaaa gatatggatg
     9061 aaaaaggtgt gatcgacatg gaagttggta tcttctcgca tccttacggt agcactcaag
     9121 aaacggaaga atcgggccaa tcggaagaaa caactgaaag tgaagaaaat gcggatcaaa
     9181 caaacgaatc aagtgaagag gcagaaacgc aaaaagcaca aacacctgga tcgtctagca
     9241 caaacggatc atctgattca aatgattcaa gtgaaacaag tcaaatagaa gataatccaa
     9301 gtacaactcc gtcaagttta aatgaaaatt gataaaacaa ctttaaaaag taagaatgta
     9361 ctttctaaaa accagtaatt tatggtagaa ttggaagcag tgaatatatc tattaataag
     9421 tatattttaa aacggacgct agttgttcgc ttgtatatgg aatagtaagt aggagggaat
     9481 cccattcatg gcaaaaacag gaatgtatgt aggccttgat attggaacga catctgttaa
     9541 agttgtcgtg gctgaatata tcgacagcca aatgaatatt attggagtag gaaacgcaaa
     9601 atcagaaggg attaaccgag gcattatcgt tgatatcgat aaaacggtcc aagcgataca
     9661 acgagcagta cgacaagcag aagaaaaagc aggtatccag atcaaaggcg taagtgtcgg
     9721 tttgccagca aacatgcttg aagtagaaaa ttgccaggga atgattgcag ttaacggaga
     9781 ttcaaaggaa atcaccgatg aagatgtcag aaatgtggct tcagctgccc ttgttcgttc
     9841 aatacctcct gaacgtcaaa tcgtgtcaat attgccacaa gattttactg tcgatgggtt
     9901 cgagggaatc aaagatcctc gtggaatgat cggcgtaaga ttagaaatgt acggtctgtt
     9961 gtttacagga cctaaaacga tcatccataa catacgtaaa tgcgtggaaa atgccggatt
    10021 aatcgtcaac gaaatggtga ttactccttt agcgctgact gaatcaattc tttctgacgg
    10081 agaaaaagat tttggtacga tcgtcattga tatgggtggc ggccaaacaa caacggctgt
    10141 tatgcatgat aagcaattga agtttaccaa tcttgaccaa gaaggtggcg aatttgtcac
    10201 taaagatatc tctattgtat taaatacgtc atttaacaat gcagaagcgt tgaaaataaa
    10261 ctacggggat gcatacccag aacggacatc accagatgaa gaatttccag tagatgtgat
    10321 tggtcagtca gaaccagtga aagtcgatga acgctacttg tcagaaatta tttcagctcg
    10381 aatggaacag attttcaaca aagcaaaaga tgcattggac caaatcgaag cattagagtt
    10441 gccaggtggc gtcgtattga caggcggagc tgctagtctt cctggtgttg tggacttggc
    10501 acaagaaatc ttcggcgtaa atgtaaaatt gtatgttcca aatcacatgg ggctgagaaa
    10561 cccggtattt acaaatgtaa taagtatcgt ggattattca gcaaacttga gtgaagtgta
    10621 tcaattggca aaaattgctg ttacgggtga aacctcagca gctcgtcaaa tggtagttga
    10681 acaagaaaca acaaacacat atgagagcta tgaagcacct gaagaaacaa tctacgatga
    10741 gccggaacca aaagagtccg gagaaaatgt aaaaaataaa atcaagggtt tctttacaaa
    10801 tatttttgat taattcatcc tgggaggaat aagatatgga attctcaatt gataataata
    10861 tcaatgacgg cgcagtaatc aaagtcatcg gagtcggtgg tggcggtgga aatgctgtaa
    10921 accgaatgat tgaagaaaac gttaaaggtg ttgaattcat tacagccaac acagacgtgc
    10981 aagcattaaa aaattcaaaa gctgaaacag ttatccaatt aggccccaaa tatactcgtg
    11041 gtttaggtgc tggttctcag ccagaagttg gacaaaaagc ggcagaagaa agtgaacaat
    11101 ccttacgcga agctttagac ggtgcagata tgattttcat cactgcagga atgggtggag
    11161 gaaccggtac aggtgctgca ccaatcgttg caggtatcgc gaaagaattg ggcgcattga
    11221 ctgttggtgt cgtgacacgt ccattcactt ttgaaggacc gaaacgtggc cgttttgctg
    11281 cagaaggtat cgctcgtttg aaagaaaatg tggatacatt attaattatc tcaaataacc
    11341 gtttattaga agttgtagat aaaaagacac cgatgcttga agctttccgt gaagcagata
    11401 atgttttacg acaaggtgta caaggtatct ctgatttgat cacagcgcca ggttacgtta
    11461 accttgactt tgctgacgtg aaaacagtga tggaaaacca aggtacagca ttgatgggga
    11521 tcggtgtggc tagtggagaa gatcgcgtag tagaagcaac gaaaaaagca atctcctctc
    11581 cattgcttga aacatctatt gatggagctg aacaagtact tcttaacatc acaggcggtt
    11641 tggatatgac attgttcgaa gcacaagatg cttcagatat cgtagcaaat gcagcaactg
    11701 gcgatgtaaa tatcattcta ggtacatcta tcaatgaaga aatgggcgat gaaatccgtg
    11761 tgacggtcat cgcaacaggt atcgatgaat caaaaaagga acgcaagtct agccgaccag
    11821 ctagacaagc acaaatgcaa tcacctgctc aaaagactgt attagatatg gatcaagcta
    11881 aaccaacttc ttcagaagaa gaaaacagtt ttggcgattg ggatatcaga cgtgaacaaa
    11941 atgttcgtcc tagagttgat gattccaatt ttgacaatat cgaaaaaaaa gaatttgata
    12001 cattcaaccg cgaagaaaca aaatctcatg gcgatgatga attaaatacg ccaccgtttt
    12061 tccgtcgtaa aaaataaagg agaggaagag cacatgattg ctgacaactt gcagaaaatc
    12121 aagcaagaaa ttcaggattc gtgtgctctt gttcatcgtg atccgaaaga agttacatta
    12181 gtagctgtaa caaaatctgt tgaatccgct caagctgaag aattggttag agctggcgtt
    12241 atgaattgtg cagaaaaccg agtagataaa ttattagaaa agaaacaaga tttatttgat
    12301 tttcctgaaa taaagtggca tttaattggt aatttacagc gcagaaaggt aaaattgatt
    12361 gtaaatgaaa ttgattactt tcatgcgttg gacagtttaa gattagcaga agaaatccag
    12421 aagcggttag agaaagaatt atcctgtttt attgaagtaa atgtttctgg tgaggaatca
    12481 aaacatggaa tttcaccaga tgagctttta ccgtttgtgg aatctttagc tgcctgcaca
    12541 aaaatcaagg ttgttgggtt gatgactatg gcgcctaaag aagcttctga tgagcaaatc
    12601 cataaagtgt ttggtacgct gaaagaattg cgtgatcttg ttcaagcaaa aggttatgcc
    12661 catgcaccat gtagtgaatt aagtatgggg atgagtcaag actttcaaat tgcgattgaa
    12721 gaaggtgcaa catttatccg tgtaggaaca gcgctattca aagacgaata aaggaggaac
    12781 aaaaatgccg cttttaaaca gaggagcaat tgctaatttt ttcggattag ctgatgagga
    12841 agagtacgaa taccaagatt atcctgaaac aacaaaaaag gctgtacagc caactaaaaa
    12901 aaatacaaca cagaatcaat caactcattc tgtatctgct gcgaatactc atgaaaaaca
    12961 aaaaacggcg acttcacgtc ctgtaacgca tgaaagtatt cagccggctg aaaagaaaaa
    13021 agtaattgaa ctacatcaat cacctacaaa aactgtagcg aaagaaagaa cagcttcaag
    13081 aacctctaca caacctaaag caaatcttgc tggaaaaatc actgtaatgg aaccaagagc
    13141 ttattctgaa gctatggcga ttgctaaacg aatcattgct ggagaagcaa ccttagtgaa
    13201 ttttcattta gtagaagaaa aacaagcgag aagaatcgtt gattttctaa caggaacagt
    13261 ttatgcacta gatggagata tcaaacgtgt tggagatgag atttttcttt gtacaccgac
    13321 tgggatggag attgacagtt caaccgctca atcttttgca gatgcgaatt tgtttgatct
    13381 gtaagaggag gaactacgat ttatactatt gcactattac ttcatttact tcaacgggca
    13441 gcctatttct atagcattat cttgatggta tatgcattgc tctcttggtt tccaggcggg
    13501 tatcaatcag ggctaggacg attattagct aagatctgcg aaccgtatat cagtttgttc
    13561 gatcgtctgc ctttgaggat tggacccttg gatttcacaa ttttaggggc aatcctgatc
    13621 ttgaacttag ccgtacaagg tttagcacag atcgttatat ggataactgt ccatttagtt
    13681 tattgattga agcaacaaaa tctttattct cagaatagat aggaggggaa aattttggat
    13741 gcaaatgtat accagcactt tcgggcagat gaacgtacat ttatcgattc ggtaggcgat
    13801 tggattgaac aggttcaagg acaatatgca ccctatctga ccgaatttct tgacccaaga
    13861 caatcctata ttttagaaac actgattcga caaagcagtg atttgcgttt catgttttat
    13921 ggcggatatg aacaggcaga aagaaaaaga tgtttgattt tccccgatta ttacgaacct
    13981 gtagaggaag attttgaaac agcattgtat gaaatccatt atcctagcaa atttgccagc
    14041 ctaagtcatg ggaaaatcct tggaacttta gtgggaacgg ggatcaaacg ctcttacttt
    14101 ggcgatatca tctcggacgg tgatcgctgg caagtattta ttgcaaatga ggtggatcat
    14161 tttgttgctg cacaagtaac aaagattgga aaggttgctg tacgcttgga accaatcaaa
    14221 tacacagaaa tgattatgcc gaaagatagc tggacacaag aaagaggaac agttagttcc
    14281 atgcgactag acagtgtgat ctctgagatt tataatattt ccagacagcg gtccaagcag
    14341 ctgatcgaag ctggtaaagt aaaagtgaac tggacagaaa atacaagacc tgattttgta
    14401 cttgaactat tagatattat ttcagtcaga ggttatggca gagtccagat ccaagaactt
    14461 gaggggaaga cgaaaaaaga aaagtacagg gtactgttcg gtgtattacg taaataaaat
    14521 aaaaaggtgg tttactcatg gctttaactc cattagatat ccaaaataag acattcccga
    14581 caaaaatgcg gggatataat caagacgaag tagatgattt cttagattta gttgttcgtg
    14641 actatgagga attgactcaa agaaatcgag aattagaaaa agcagtcaaa cattctgaag
    14701 aaaaacttga gtacttcaat gaattaaaag atgctttgaa ccaatcaatc atcgtagctc
    14761 aagatacggc tgataaagta aaaacaagtg caagcaagga atccgaagtg atcgttactt
    14821 ctgcacaaaa caaagcagat gagttagttg cgaacgctga aaaacgtgca catcaattaa
    14881 caactgatgc agaagaaaaa gcaagaaaaa tcttgacaga tgcaacagaa aaagcaagac
    14941 agcttgcgac agaaactgaa gatcttaaga aaaaaacacg agtattccat caacgtatca
    15001 gcttgatgct tgagtctcaa ttagaacaag tgaaaagtcc ggaatgggat gaaattttac
    15061 aaccattctc cagctacgtg actgacagcc atgaagtgat caaagaagtt ttagcaaaac
    15121 agcttgacaa tgaaaatgat actgatgtaa actcagacac aggagtcata gaagcaccta
    15181 tgacggcatt tgatacacag gctattgata tctcgatcgt gcctggtgaa aatgaaaacg
    15241 agtagaacag aaaagcactt tgttattctt aaagcgagcc gatgagagtg gaagttcggt
    15301 aagacactgc atggtgaaag atcctctact tgagacctgc ttttccgaaa ttagagtaag
    15361 aagcagcggc tgacctcgtt attggtcaca gagagtttgc actagataga tgcaactaaa
    15421 tttgggtggt accacggcat gttcgtccct tgcggctgaa catgcttttt tgtgtccgga
    15481 aaacaaataa aggagtgtat ggttgaatga aaatgaaaga aaccttgcaa ttaggaaaaa
    15541 caaaattccc tatgcgtggt aatttgccaa accgagaagg cgagtggcaa aaagaatggg
    15601 aagaaaatcg aatctaccaa aaaagacaag agttgaacga aggaaaacct agctttgttc
    15661 ttcatgatgg acctccatat gccaatggta atatccattt aggacattca ttgaataaaa
    15721 tcagtaaaga cattatcatt cgttcaaaat caatgtctgg ctatcgtgca ccatatgttc
    15781 caggatggga tacacatgga ttgccgattg aacaagtatt ggctaataaa ggcgtgaaac
    15841 gtaaagaaat gtccatggca gaatacttgg aaaaatgtcg tgaatatgca ctttcccaag
    15901 tagataagca acgagcagat ttcaaacgcc taggagttgc aggagattgg gaacatcctt
    15961 atgttacatt agatccttct tatgaagctg cacaagtacg cgtatttggt aaaatggctg
    16021 aaaaaggcta tatttacaaa ggattgaaac caatttactg gtctccgtca agtgagtctt
    16081 ctttagcgga agctgaaatt gaatataaag atgtaaaatc tccatctatt tttgtagctt
    16141 tcaatgtcaa agacggcaaa gatattcttg atgaagatac agcttttgtc atttggacga
    16201 caacaccatg gacattgcca gcaaaccaag gtattgctgt aaatccaaaa tttacgtatg
    16261 tattggtgga agccgatggc cgtaagtttg tcgtagcgaa agatttgcta gaaacagtcc
    16321 aatcagaaat cggttgggaa aatgtaacag ttctaaaaga atttgctgga caagaaatgg
    16381 aatatatgac tgcaagtcat ccattctatg aacgtgaatc acttgtgatt ttaggcgatc
    16441 atgttacttt agacgctggt actggattag tccatactgc tccaggacat ggggaagatg
    16501 actatatcgc tggtagcaaa tataaactat ctgtagtgtc acctgtagac tcaaaaggat
    16561 tgtttacaga agaagcacct ggatttgaag gtatctttta tgacaaagca aatcctatga
    16621 tcactgatct attaaaagaa aaaggagctt tgctgaagct agatttcttt actcatagtt
    16681 atcctcacga ttggcggacg aaaaagcctg tgatctatcg ggcaactcca caatggtttg
    16741 catctatcga taaattccgt cagaatattt tggatgaagt agaaaaagta gagtgggtta
    16801 tcccatgggg taaaacacgt ttgtataata tgatccgtga ccgcggcgat tgggtaattt
    16861 cccgtcagcg tgcatgggga gtaccacttc cgattttcta tgctgaaaat ggagaagcaa
    16921 tcatcacacc agaaacaatc gaacatgtgg caaaattatt tgctgaacat ggatcaatta
    16981 tctggtttga aaaagaagcg aaagaacttc ttcctgaagg atttacccac ccaggttctc
    17041 ctaatggcga atttacaaaa gaaaaagata tcatggatgt ctggtttgat tctggttctt
    17101 ctcatgaagc tgttttacgt cagcgaccag aattatcatt cccagcagat atgtatttag
    17161 aaggatcaga ccaatatcgt ggctggttca actcaagtat cacgactagt gtggctatca
    17221 atggtgtagc accatacaaa tcagttattt ctcaaggatt tgctttagat ggtgaaggcc
    17281 gtaagatgag taaatcttta ggaaacatca ttgcaccaga aaaagtgatc aaacaatttg
    17341 gtgcagatat ccttcgctta tgggtatcta gtgtagatta tgaagcagat gttcgtgttt
    17401 ctatggatat cttaagtcaa gtggcagaag tatatcgtaa aatcagaaat acgatgcgtt
    17461 tcttattagc gaatacagaa gatttcgatc ctaaaaaaga tactgtttcg tacaatgatt
    17521 tacgttctgt agacaaatat atgaccgttc gattgaacca agtaatcaaa gaaatccgag
    17581 aagaagggta cgacaaatat aacttcatgc atatttaccg tacagttatg aacttcttga
    17641 cggttgatct atcgtcattc tacctggatt ttgctaaaga tgtagtttat attgaagcag
    17701 aagatgatta tcaacgtcgt tgcatgcaaa ctgttttcta tcaaacagct gtagctttga
    17761 caaaattatt gactcctatc attccgcata cagcagaaga aatctggtca ttcctgaaag
    17821 aagaagagga gtacgttcag ttaagcgagt ttcctggtta tgaagagttt gcaaaccaag
    17881 aagaattgat ggatacctgg actgctttca tggacttccg tgataatgtc ttaaaagcat
    17941 tggaagaagc aagaaacagc aaattgattg gtaaatcttt ggaagcaaaa ctaactgttt
    18001 atccaaaaca acaagttcgt gaaatgttaa aagctttgga tgcagatatt gcacaattat
    18061 tgatcgtttc accagactac tttgaagtta tggcagcaga tgaacaagtg ccagataacg
    18121 ctatggtatt tgatgatgtt gctattacag tggaaaaagc agacggggaa acttgcgatc
    18181 gctgtcgcca agttcgtaaa gatgtaggag tagatgaaaa acttcctcat ctatgcggtc
    18241 gttgtgcaaa aatcgttgaa gacttctatc ctgaagctgt agctgaagga ttcgaagaaa
    18301 agtaaaaggg aaaagggggt gtgacatgtt ctttgtcaca cccccttttg ttatcaatcg
    18361 tttaattttc cacgttcacg ataccagtta tctggttgcc agatccattc gaatccgtct
    18421 ttttcaagta gttcgaaagc tgctttaggt cccattgatc cagctttgta gttgggaaaa
    18481 tccggttgtg tttggtccca agcttttcga atgacatcca cgatatgcca agattgagcg
    18541 acttcttccc aatgagagaa gttcgttcca tcaccattca acgcatctaa tagtaatttt
    18601 tcgtaagctt ctgggctatt ttcaaccatt tctgcactat ttctaaattc caatttgatt
    18661 ggttccgttt caaatccttg ccctacttct ttcccattca aggataaaga aaatccttca
    18721 gttggctgaa tatagatcgt tagaacatta ggtggaagtg tcgtatcgtc acatggttca
    18781 tcgacagatg ttttgaatac attaacagga acttgtttga atacgatgtt gatccgagtc
    18841 cctttttcag tcaaacgttt gcctgtacgt acatagaaag gaacacctga ccaacggaag
    18901 ttatcgatag tgaatttgcc tgcgacgaat gtttcagtct ctgattgagc atccactttg
    18961 tcttcttcgc gatagccctt gaattgtaca ccatccaatt caccttgagc atattgcccg
    19021 cgaacgaagt tttctaacac ttcttcttcg ttataaatgc ggatagcttt caatgctttg
    19081 atcttttctg tacggatttc tttttcggaa aatgctactg gcggttccat tgcaagtaat
    19141 gctacaacct gtaaaatatg attttgcacc atgtctttaa gtgcgccact atgatcgtag
    19201 tagcccccgc gatcttctac accgaggttt tcgccaaaag taatttggat attgtcgata
    19261 tagcgattgt tccattgagc ctcaaaaata ttattggcaa aacggatagc tgagatgttt
    19321 tggatcattt cttttcctaa ataatgatca atcctgaaaa tatcttgttc tgggaatact
    19381 tctctgattt gctggttcaa ttcaaaagca gactggtaat cagaaccgaa cggtttttct
    19441 atgatcaaac gattgaagcc attttttgtc ataatatttt gtgatttcaa atgagaaacg
    19501 atcgttccaa aaaattgagg tgccatagcc aaataataga cacggttacc gtttaattga
    19561 tagtgtccat ctaatcggtc agacaattct tttaatgtct gataatgcgt tgaatcattt
    19621 acatcatggg attgatagta aaaatggctt gcaaaggtag ttgcttcttc atctgaagga
    19681 ttcaatgcac ggattgtttc tttgacaact tcacggtagt gctcatcgct ccagggacgg
    19741 cgagcggttc cgattacagc aaaatgctcg cctaaatttc cttttctgta aaggcggaac
    19801 aaagacgggt acaatttacg ctgggctagg tcacctgtac cgccgaatat cgtaaacaat
    19861 acattttttt cattagacat gtggatacac tcctttatat tttatcgaca aaaatcgtgc
    19921 tggccgcctt aaagctgacg gggattttct ttctatcatt ttggatagtg atcggtcctt
    19981 cataagctgc gatgtcaacg atctcatatt cttcgttgat attcaggtca atagtgacta
    20041 agtaatccaa caattctttt tcatcaagca ctcgtgcgat acgcactttt gtaccgacag
    20101 gataggactc caatgtttgt cggcgttctt catggacaac ttcctctttt ttaggaatcg
    20161 atcctccatg aggacaatgt tcaggatgat tcaaatagtt ttctaaatga tctgcaagat
    20221 gttccgaagt cacatgttcc aatacttcag catcatcatg gacttcattc cagctatagt
    20281 ctaaatgttc aactaaaaaa acttcccata agcgatgttt gcgaatcaga ctgctagctt
    20341 tttgcagtcc gcttgcagtc aattgtacac cttgataagg ggaatgttct acaagctctt
    20401 ctttcacaag tttagaaatc atttcactga cagatgcagc tgagacatct agactagata
    20461 cgatttgttt attgttgact ttcgttgtat ctccacctag ttctaaaatg attttcaaat
    20521 aatcttctcg atttggtgtc atattcgatc atcctttcat ggtaaaaaac catcattagt
    20581 aagtgtaaca aaaaaaacgt taattgacta tttttctaaa taaaaaacaa aggattttta
    20641 gacgttttta caacaaaaaa cgaaagatga tagagaagac tcattctttc gtttataaag
    20701 gatatatata tacattggga gggatctatt tttcagataa caacttatat gaagtgtctg
    20761 cagattcgtc ataaagagtt tgttcgggat aagaaaagat aagataaaaa ccatactgat
    20821 cacgtacgac taaacgatat tcatctattt ctaccacaga tgtgccaaga gactgaccgt
    20881 tgatcaaaaa cgtgtaatca gatgtaatct gtaattgttg tttttgctgg ctatcactaa
    20941 agaaccatgt accttgatac tttgagagtc cttctgactt ttccgcttgt tttttctttt
    21001 tcaagtaaat aaaacttgag agaacggtaa tgagtgaacc aagaaaatag atcatttttt
    21061 ttatcaccaa atcactcctt tatgagaaaa taatcgcata gacatattct ttcttcttta
    21121 tttcattgta ccgaataaaa gctaaaattt ctctaagact ttcgactggt ttttttactg
    21181 tttttctaca aattatgata ggatacatag taggaagaaa ggtgacataa tttgaagtat
    21241 cagcaagtgg aaatagcttt ttttattgac cgcccgaatc ggtttatagc acattgtatg
    21301 aaaaaagacg gagaaataat tattgcgcat gtcaaaaata caggtagagg aaaagaggtc
    21361 ctgataccag gcgctgaagt ggctattgta tttgctcctg gacctaaacg gaaaaccgcc
    21421 tatgatctga ttgctgtaaa aaagaagggt gactggttta atatcgatag tcaattgcct
    21481 aatcggctgg caattgatgg tattttagac ggcacgatcc ttttaccagg attaactggt
    21541 ccatttacgg tatacaagcg tgaagtaact tatcttcact ccaaatttga tatttattta
    21601 gagacggtgg tcggacaaaa agcatttgtt gaagtcaaag gtatgacact tgaaaataaa
    21661 gcgattggtg cttttcctga tgcgcccaca ctcagaggat tgaaacatat aggagaatta
    21721 acgtatgccg cacaagatgg ttatgccgct tatgtcttgt ttattgctca gttcgaacat
    21781 cttcatctgg caacgatcca tgaagaaatg caaccagcac tagctgagat ggttcgacat
    21841 gcgcagcaaa gcggagttca aatcctcgcc tataattgtc aagtatcggc agatgaggta
    21901 actgtcaagc aggccattcc atttgatgta gatatccctt ttgaagatcc tatacaatag
    21961 aaagaaggta aaataatgat caacttaggt attatcggaa caaattggat tactcatcaa
    22021 tttgtccaag cagctttaag cacaaaaaaa tatgaattga cagcggtata ttctagacat
    22081 ttggaaaaag cacaggaatt tggttctcat tatgaagggg atatcgaatt cgcaacagat
    22141 ttgaagactt ttttcaatat tactcatatg gacaccattt atattgcttc tccgaatagt
    22201 cttcacttta gtcaagctaa gcaagcaatt ttggcaggaa aaaatgtgat agttgaaaag
    22261 ccagcttttt cgacgccaaa agaaatggat gaaatcatcc atttggcaaa tgaacacaat
    22321 gtgtattttt ttgaagcagc aagaaatatc catgaatcag gttttaaaaa gattgccgat
    22381 tacttaccat taaaagacga gataatcggt gcaaatttca gctatatgaa atattcttcg
    22441 cgctatgacc aagtattaga tggagaagaa ccgaatatct tctcgactca tttctcaggt
    22501 ggtgcattga tggatttagg tgtttacctt gtatatgcag ctgttgcttg gtttggaatg
    22561 ccaaaagaag tccattattt cccaagaaag atttctactg gtgtagatgg tcttggatgg
    22621 gggatcttac gttatgagaa ttttgatgta tcgatccagc caggtaaaat cggtgattct
    22681 tatttaccgt cagagattta ttttgactca ggaacgctag tgatgaatgg agtcaatgca
    22741 attgaacaag cacaatttca tgatcgcgcg cataaagaaa tcattgattt ggcggtaaca
    22801 gctgcagaaa atccaatggc agatgaagca gaggattttg ccaatgtcat ggctcatccg
    22861 aaagatccag cttggggatt gttgtatgaa gagtggatcg agctagctag aaatgtgaac
    22921 caagtgattt atgatttaag aaaaaatggc ggcatcaaat tcgatgcaga taatgaggaa
    22981 gatttttaat gaacgaattt gtacaagtat ttcccgaaaa ttggcagaaa aaatatgagg
    23041 ataaagggtt tatctcacct tctcttattc aacgagcagt ttttcagcca ctaagtaata
    23101 aacaaagtat agtagcggtt tctcctacag gttcaggtaa aacactggct tatctttggc
    23161 ctctactact aaacgtggaa ccaggtgaag caagtgcgtt ggtcattttt gcttcttctc
    23221 aagaactagc tattcaagta gcagatgttg cacgtgaatg gggaaaagac aaagagttga
    23281 aagtccaatc tttggtgggc ggagccaacg tcaaacgtca gattgagggt ttgaaggaaa
    23341 aaccagaaat cttgattggt acaccaggaa gaatattgga attgatgaaa gcaaaaaaaa
    23401 taaaagcaca tcaagtgaaa acaatggtat ttgatgaagc agaccagcta tttgacgctg
    23461 ggaacagcca aatcattgat caaattcttc atcaagctcc aacagagtat cagctagctt
    23521 ttttttcagc gacagcagat cgttcccttg aaagcattga aaaaatcact gggaaaacgt
    23581 ttgagacaat tgacgtgaca gcagaggacg attctcgaaa aggactgaga cattactttt
    23641 tacgtgtacc gatacgaaag aaagatgact acttgagacg tctcacgcat atagaagatt
    23701 ttcatggtct gattttcttt aatcaactga ctgaattagg cgtaatggaa gaaaaattgc
    23761 tgtatagagg tgtaccggta ggaagtctgg cctctgatca gaacaagcta ctccgaaaag
    23821 cagcgctaga acaattcaaa aagaaaatgg tcagagcact tttgaccaca gatgtggcgg
    23881 ctagaggttt agatattaca ggtcttcctt atgtagtgaa tgcagaagtt ccgttaagtg
    23941 aagaagctta tttgcatcgt tcgggaagaa ctggtcgaat gggtaatgaa ggaatagtaa
    24001 ttacactcgt acaagataat aatctaagag atttaaaacg gcttttacgt ccatcaaata
    24061 taacacttga agagattttt ttatacggag gtaagctgca aactgagcag ccaaccaaag
    24121 aagatgcagc tcctgcagta aataaaaagt atgcgaaaac tttttctgga aaggagcaga
    24181 acccagaata taacaaagaa aaaataagaa aaaaagttaa aaataaatca aaaaaagaga
    24241 gaaataaagg agcaaggcgc aaataagcgc ctttttatgc tatgattatg aaaacgggaa
    24301 caaaattggt tgccagagga ggggttatgt gaaagaacgt caagaagaga tcttgcagct
    24361 attgacagaa aaccaacaag gattgacagc aagtgatgta gctgagcgct taactatcga
    24421 tcgaagcaat gcaagccgat atttaagtga actatacaag gcccatcata ttgtaaaaac
    24481 cgctggccga ccagtcgttt actctttgcc aacggaaaag tcaaaatctg atgaagtgca
    24541 tgtcgactct tctacgcaag tgacttttga aacactcgtt ggagaaaatg attctttaaa
    24601 agtcagcata cagcaggcaa aagctgcgat tctttatcca ccgcgggggt tgcatacgat
    24661 tatttttgga gaaactggta cagggaagtc gatgtttgct gaatgtatgt atcattttgc
    24721 aatcgattca gagatgcttt ctgcagatgc gccatttgtt tctttcaatt gtgcggatta
    24781 tgctcaaaat ccgcaactgc tctttggaca catctttggg atcaaaaaag gggcttacac
    24841 aggagcagct caagatagtc ccggtctaat agccaaagca gacggcggta tattgttttt
    24901 agatgagatc caccgtttac cgccggaagg gcaggaaatg ctgtttactt tcattgataa
    24961 aggtatttac cgtccgcttg gagaaagtgc acaagtacat gaagcatctg ttcagatcat
    25021 tggggcgacg actgaatctt cagaaagttt cttatctacg ttcaatcgac ggattcctat
    25081 ggcaatcacc ttgccgaatc ttgcttcacg ttcactggat gagcgttatg aaatcatctc
    25141 tctttttatc aaacaagaag cgaatcgttt gaatcaacgt atcgatatcg aaaaagaagc
    25201 gattcttgcc ttcatgcttt atgatgcgga aggaaatatc ggtcaagtga aacgagactt
    25261 gaaactagtc tgtgcaaaat cttttttaca ttaccgtact catcatgaga aaaagctaat
    25321 cattcgaaaa gaagaacttt cccttcaagt acaaaaagga ttactgaaaa tcaaggaaat
    25381 accggaacgt ctcgaccgat tcatggacag caaaagtcag tatttgacat ttgaaccagg
    25441 atttgctgat gttgtctggt cacaagatcc agaacgaaac atgcaggtct ataacgatat
    25501 tgaggaaaaa gtcacttctc tttccgaaac cggtgtggag aacattgatc tagagaccct
    25561 catttcaaaa gatgtggatg cttatttcca gacatatgtc aaagaattga ctaaaagttc
    25621 gattcccaaa gatcttttgc cagatgaaat ctggcggtta accaatcaat tgtatgatgt
    25681 ggctgaaaaa cggttagacc gtatttataa tgaaaaagct cgttttgctt ttgctttgca
    25741 tctgcaaagt acattggatc gtgtgagaga aggtcatatg atcgtccacc cagatttgaa
    25801 taacgtacgg aaaaatttta agagggaatt ccaggtagca atcgatcttt caacgatcat
    25861 tgaagaagaa gaacaagttg agattccttt tgatgaaatc ggctttatta gtatgtttct
    25921 ttctatcaat ctaggagaaa gtgagcctgt taaagaaaac aaagtagatg ttctcatatt
    25981 gatgcacggc agacagactg cttcaagcat gttggagatg gcacaagaac tattaggaac
    26041 aaaagtaggg aaagctttta atatgccact ggttatggaa gttcaaacaa tgtatgaaga
    26101 agtgttacaa tatgtcaaaa actatcaaga gcatttgacg aatggcctac tcttgttaac
    26161 agatatggga tcattaaaca ccttcagtga tttgatttat gaagagactg gcattcggac
    26221 gaaagctatc acgatgacaa gtaccatgat tgtgatagaa gcgatacgga tggcaagtgt
    26281 tggaagaagc ttagaagata tttatcaaaa tatccagctt tcctttgaat cgatcgtacg
    26341 tgatcagttc aaagcggaaa agtctgaaac tttacagcgt aaaaaagccg taatcgtgac
    26401 atgctttact ggtgaaggag tagctgccaa attatatcag cgtattatgc cagtgattaa
    26461 tcaaaataag gtagaactga tccagatgca gtttttagaa cgtgaatcat tcaaaaaaca
    26521 catcgatgcc ttattggaag aatatgagat caaagcaatt gcgggaacag ttgatgttga
    26581 ttatcaaaat atcccatttt tttcagccta tgacgttttt gatgatgaaa aactaaatat
    26641 tttaaagcgt atagctgatg atgagatccc gacagctaaa attgtcgatt cacttgctgg
    26701 atcattaagc gatgtctttt ctattgatga actgatccgt tcattgcaaa agacagttca
    26761 gcagatccag accgatttgt cgattatcgt ggaacctgga gtagatactg gaatcgttat
    26821 ccatttagcc ttcatgttag acggattgat caaacaagaa agccatcggc aatttgagaa
    26881 actgtctatt ttctcaaaaa tttatcgttt ggaaatggat atcgttcggg caaacttgat
    26941 ggcactagaa cgaaaatatc aggtaaaaat tccagaagat gatatcgcat ttcttacaca
    27001 aatgtttctt gaaaataaaa taaaaaaatg aacacactaa tcatactgtt tacacactaa
    27061 acatctgtgt gcgcactata aaaaagagct aaaaagcatg aaaacgctat ttcagctctt
    27121 ttttttattg gcatgcttct tgcgttatat aaaagtgtaa aaaaataaaa ggagtgttaa
    27181 aaatggcaaa aaaaacaatc atgttagttt gttcagcagg gatgagtacg agtttattgg
    27241 tcacaaaaat gcaaaaagca gcagaagaaa aagggatgga agccgatatt tttgcggtct
    27301 ctgcctcaga tgcagataat aatttagaat ctaagaatgt cgatgtatta ttattaggtc
    27361 ctcaagtacg tttcatgaaa gctcaatttg aacaaaaact tgcgcctaaa ggtattccat
    27421 tagatgtcat caatatgcaa gactatggca tgatgaacgg agaaaaagtt cttgcacaag
    27481 cagaaaatct aatgaaataa atcaagtatg cagttggggg tataacaacg tggaagatca
    27541 acaaaatcta gaagccatca tggggttaat catgtttggc ggtaatgcaa agagcgatgc
    27601 aatggaagcg attgcggctg ctaaaatcgg tgattttgag ttagcggata ggaaaatcgc
    27661 tgatgcagaa gaatcactta tacaagcgca tcatgcacaa acagacatgt tgacacaaga
    27721 agcacaagga aatcatatgc aagtgacatt attgactgtt cacagtcaag atcacttaat
    27781 gacatctatt gcgtttactg atttagcaaa agaaatcatc gaactttatc gtcgcgtata
    27841 cagcgaataa atacaacttc ttttttgaac ggaagaagaa aaatttttga acggattttt
    27901 tattttgtta taaggagaac tctctttata taaaaaactt ataggaggaa gtaaaaaatg
    27961 aacggactat caagttggat ggagaaatac atcctgccgg ttgccgcaaa aatcggagcg
    28021 caaaaacatt tagttgcttt gcgtgatgct tttatcggca cactgcccgc aacaatggcg
    28081 ggatcagtcg ctgtaatgat caatgcaatt attcgtgatt taccgcaaca gtttaacagt
    28141 acctatgctg acagtttagc agcagacaaa ggaatctttg ccgtattgaa tgtcatcatc
    28201 ggaatcaatg gttttgtctg gaacggtaca ctagcaatcg ccggattgat ctttgccttt
    28261 tcttgggggt ataatcttgc ccgagcttat ggagtaaatg atctagctgg ggggatcgtt
    28321 ggtttagcta ccttgattca aggtatttcc tttggtccag cattatcagc agcattagat
    28381 gtgaatgttc cttcaaatgt cgcagatgca atcaatagtt ctgagattgg tgcaacgatt
    28441 gcagatggca cactgtcagt tggattatgg ggttggctaa aactagatca tttgaatgga
    28501 aacgcttatt ttacagttat gatcatggga gctatttcag tcattatttt ttgtaaattg
    28561 atgcttgcca acattacgat caaaatgcct gattcagtac caccagcagt atctaaagca
    28621 tttgcagcca ttttaccagc gacgatcgct ttatacgtca ttgctattat caattatatc
    28681 gtaggtcgtg tagctgatgg gcaactgatc attgatttgg tgcaaaaata tattgcagaa
    28741 ccattcctag gattatctca agggttggga gcagtattga tcgtcacagt atttgttcaa
    28801 gtattctggt tctttggtat tcatggacca aatgtattgg caccagtgct agagggaatc
    28861 tggggacaag cacaactaat caatattgat attttccaaa aaggttataa cggaaaaact
    28921 ggtacaccag ccgtgttaga tgcaatcgaa gatggaaaag cttatatgtg ggtccgcgga
    28981 tcgttcgatg cttttgcatg gtttggcgga tcaggcggaa cgatcgtctt actgatagct
    29041 atcttgattt tctctaaacg tgcagattat ctaacagtag ctaaattatc cattgggcct
    29101 ggtatcttca atatcaacga accagtgatg tttggtctgc caatcgtatt gaatgcaatc
    29161 atgtttatcc cattcttgat tgctccagta gtagcaacag caattggatg gacagctact
    29221 tatcttggcc tagtcgctcc ggtatcgcag caagtcacat gggtagtacc gccgttctta
    29281 ttgtcattct tagcaacagg agcggattgg cgggcaccga tcgtaacatt agtatgtatg
    29341 gtcgtgactt tcttgatttg ggcaccattt gtcatctctg caaacaaaat ggaccctgcc
    29401 gcaggcgaac aacaataaga tgaacgtgag gagcgggacg ctttaagttt cgttcctcat
    29461 aatgaatttt cgaggaggaa ctgaaatgat tacaattgcc agtgcaatgc aacaagatga
    29521 aatcaaagaa ttgttagaag gatacaatga agaaggaatg acttttacat ttaaagaaaa
    29581 acaaggaatc aagctgttct ttgaaacaag tgcagaagat ccggaagttg cggctaaaaa
    29641 agcaaaggaa ctaatcaaag cacagccttg gggaacagtc ctttattttc aatcaacagc
    29701 tgtttagaaa atggtttaaa aagataggat ggttgagatg ataaaaaacg gtttattttt
    29761 atgtagtatt ggttctttga tcgtgttgta tagtttgaat ccacattcgc aaaggtatga
    29821 tttgtatagt atggtcacag gtctttttct aacaggatta ggaatctatt tcttcatgaa
    29881 ggggaaaaaa agagaagaag caaaggagaa aaataactaa tggaaatcac gaaaaaatta
    29941 aatattccag catcgttttt ttacgataaa gttatggact ctgtcctttt cgatgtacgt
    30001 aaagcgaccg gcaaatcagt aactagaaaa cagctgaaaa atttggagta cgtcaaacaa
    30061 ttctcgcaaa ataaccgggc tcgcataaaa atcgaagaag tcattgaaaa ccagtcttat
    30121 aagtttcgta catcgacaac gaagaatgaa ttcgtcgttc attatcaaat caaaccattg
    30181 gacgattcga cctgtgaggt tcattacaca gaaaaaatgg agtcttatgg catgcttcaa
    30241 aagttgaatg atatgctttt aggaacaatc ttgatgtatt ttaaacggag acgtttcaaa
    30301 aaaatgctag atatgatcga agcaacgtat taaatcaatg aaacatggta ctatttagtt
    30361 attgaaaaat gaagtaagct agaaggaatt tttcatagga agatagatct ttcctgcgaa
    30421 tcctcactag cttttttgta ggaaggggca gcaaaatgac aaacaaagta agagagctag
    30481 gtgtatcttc gattgaggaa atgtttgatt tagccactta tgcatttaat ggggcagata
    30541 cacctgaaag gcgagaacgt tttcgtacac ttgcggagaa ttcttggaat tacggatttt
    30601 ttgatgagga ggatcggcta acaagtcaag tcatggcgac cccttttcca gtgaattttt
    30661 acggacaaga gtacctaatg gccggcattg gctatgttgc ttcttatcca gaagcaaggg
    30721 gacaaggcgg gatcaatcag atcatggaaa agatccttga agattgccgt gatcgtaaag
    30781 taagcttatc ttatctggcg ccattctcct atcccttcta tcggcgctat ggctatgaac
    30841 aatcatttga taaaatcagt tatcaactaa acagtcgtga tgtgccgttt atcaaaaaaa
    30901 aatctggaaa aataaagcgt atggattttg aagatgcaaa agctgcaatc agaagaattt
    30961 atgaacagat gccggaaaac caacgagctg gtctgaagcg cgaagactgg tggtatacgt
    31021 ataaattcaa gcaaaaaggc aattaccagt ttgctttgca ttttgatgat aatgaggaag
    31081 caacgggata tatcgtttat cagcttgcaa cgtcttcctt tattattgcg gagtggggat
    31141 ttttggatca tgatgctttt tgcagccttg ttcgcttcgt aagttctcac aatggtgctt
    31201 ttgaaacatt tgaatattca tgcggctacg gcggtaatga tcaaaattat ctattagaaa
    31261 atccatttgc ttctgtaatg atcacacctt atatgatggc gaggatcgta gatatttctt
    31321 tgtttttgga aaattatcca tttaaaaaga gacaagctgc atttgcccta caaatagaag
    31381 aagatcatta tgcgaaatgg aatgaaggaa tcgtggaagt aacaatcgaa aatggaaaaa
    31441 atcatctctc aaaagtagaa caaacaaaac tcccaatcgt tcgaggaagt atccaacata
    31501 tcacgcagct attattaggg tatcgtaaag tggaagagtt ggtattccaa gaaagaatac
    31561 aggtgacaaa agaatatgaa gaaacgttaa gctcaatctt gccacagcaa cagccaattc
    31621 ttaatgacta tttttgataa aaaacgggtg atgacaatat ttttgtcacc acccttttgt
    31681 ttttcatttg gcatttgaag gaaaatgtta aagtaaatcc tcttttttcg taaatttgaa
    31741 ttttttgttt tcttttgttt tagggttttc ttttttgtgg tcttggattg cttctttcgt
    31801 aatcttttcc cgattttttt tagatgaatc taagcgttct gcgtatactt tttcttttgg
    31861 cttatcattc attggaaagg acctccctac attatttcat tctacctcta gtttatcatc
    31921 tgttaaggac tagtggaaac gttttgccta acttataagc tgattttttt agcagataag
    31981 cttgcaagta aaaaacaaac aagctacaat tgatttattg aaattgttcc tgtagctcag
    32041 taggatagag caatcgcctc ctaagcgata ggtcgctggt tcaattccag tcaggaacgt
    32101 acaaataacc cctgtatcct ttgtgataca ggggtttgtt ttgtgtttgt gccaaaaatg
    32161 tgccaaacta gttatcattt tta
//
LOCUS       DAIGOK010000017        31727 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.017, whole genome shotgun sequence.
ACCESSION   DAIGOK010000017 DAIGOK010000000
VERSION     DAIGOK010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.017"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..45
                     /locus_tag="NOA01_000606"
     CDS             <1..45
                     /locus_tag="NOA01_000606"
                     /inference="COORDINATES: protein motif:HMM:NF019687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631829.1"
                     /translation="KSIYHWDFYALLGF"
     gene            61..864
                     /locus_tag="NOA01_000607"
     CDS             61..864
                     /locus_tag="NOA01_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCI0631830.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            1099..1335
                     /locus_tag="NOA01_000608"
     CDS             1099..1335
                     /locus_tag="NOA01_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631831.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1342..3294
                     /locus_tag="NOA01_000609"
     CDS             1342..3294
                     /locus_tag="NOA01_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HCI0631832.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3366..3863)
                     /gene="dfrG"
                     /locus_tag="NOA01_000610"
     CDS             complement(3366..3863)
                     /gene="dfrG"
                     /locus_tag="NOA01_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HCI0631833.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            4225..5265
                     /locus_tag="NOA01_000611"
     CDS             4225..5265
                     /locus_tag="NOA01_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCI0631834.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            5363..5551
                     /locus_tag="NOA01_000612"
     CDS             5363..5551
                     /locus_tag="NOA01_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCI0631835.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(5609..5962)
                     /locus_tag="NOA01_000613"
     CDS             complement(5609..5962)
                     /locus_tag="NOA01_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCI0631836.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            6493..6915
                     /locus_tag="NOA01_000614"
     CDS             6493..6915
                     /locus_tag="NOA01_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCI0631837.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            6912..7142
                     /locus_tag="NOA01_000615"
     CDS             6912..7142
                     /locus_tag="NOA01_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCI0631838.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            7640..7840
                     /locus_tag="NOA01_000616"
     CDS             7640..7840
                     /locus_tag="NOA01_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HCI0631839.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            7867..9060
                     /locus_tag="NOA01_000617"
     CDS             7867..9060
                     /locus_tag="NOA01_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCI0631840.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(9128..10693)
                     /gene="guaA"
                     /locus_tag="NOA01_000618"
     CDS             complement(9128..10693)
                     /gene="guaA"
                     /locus_tag="NOA01_000618"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCI0631841.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(10752..11675)
                     /locus_tag="NOA01_000619"
     CDS             complement(10752..11675)
                     /locus_tag="NOA01_000619"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCI0631842.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            11871..12476
                     /locus_tag="NOA01_000620"
     CDS             11871..12476
                     /locus_tag="NOA01_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCI0631843.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            12784..13800
                     /locus_tag="NOA01_000621"
     CDS             12784..13800
                     /locus_tag="NOA01_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCI0631844.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            13941..15242
                     /locus_tag="NOA01_000622"
     CDS             13941..15242
                     /locus_tag="NOA01_000622"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HCI0631845.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            15288..15950
                     /gene="deoC"
                     /locus_tag="NOA01_000623"
     CDS             15288..15950
                     /gene="deoC"
                     /locus_tag="NOA01_000623"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCI0631846.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            15971..16369
                     /locus_tag="NOA01_000624"
     CDS             15971..16369
                     /locus_tag="NOA01_000624"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCI0631847.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            16435..17517
                     /locus_tag="NOA01_000625"
     CDS             16435..17517
                     /locus_tag="NOA01_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HCI0631848.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            17729..19291
                     /locus_tag="NOA01_000626"
     CDS             17729..19291
                     /locus_tag="NOA01_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0631849.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            19284..20417
                     /locus_tag="NOA01_000627"
     CDS             19284..20417
                     /locus_tag="NOA01_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI0631850.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            20417..21373
                     /locus_tag="NOA01_000628"
     CDS             20417..21373
                     /locus_tag="NOA01_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI0631851.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            21472..22638
                     /gene="deoB"
                     /locus_tag="NOA01_000629"
     CDS             21472..22638
                     /gene="deoB"
                     /locus_tag="NOA01_000629"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCI0631852.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            22662..23480
                     /locus_tag="NOA01_000630"
     CDS             22662..23480
                     /locus_tag="NOA01_000630"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCI0631853.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            23497..24204
                     /gene="deoD"
                     /locus_tag="NOA01_000631"
     CDS             23497..24204
                     /gene="deoD"
                     /locus_tag="NOA01_000631"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCI0631854.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(24502..25188)
                     /gene="gpmA"
                     /locus_tag="NOA01_000632"
     CDS             complement(24502..25188)
                     /gene="gpmA"
                     /locus_tag="NOA01_000632"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCI0631855.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(25360..25941)
                     /locus_tag="NOA01_000633"
     CDS             complement(25360..25941)
                     /locus_tag="NOA01_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCI0631856.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(26074..26757)
                     /gene="rpiA"
                     /locus_tag="NOA01_000634"
     CDS             complement(26074..26757)
                     /gene="rpiA"
                     /locus_tag="NOA01_000634"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCI0631857.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            27076..27489
                     /gene="rpsL"
                     /locus_tag="NOA01_000635"
     CDS             27076..27489
                     /gene="rpsL"
                     /locus_tag="NOA01_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCI0631858.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            27573..28043
                     /gene="rpsG"
                     /locus_tag="NOA01_000636"
     CDS             27573..28043
                     /gene="rpsG"
                     /locus_tag="NOA01_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCI0631859.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            28137..30224
                     /gene="fusA"
                     /locus_tag="NOA01_000637"
     CDS             28137..30224
                     /gene="fusA"
                     /locus_tag="NOA01_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCI0631860.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            30396..31583
                     /gene="tuf"
                     /locus_tag="NOA01_000638"
     CDS             30396..31583
                     /gene="tuf"
                     /locus_tag="NOA01_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCI0631861.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
BASE COUNT        10174 a         5729 c         6583 g         9241 t
ORIGIN      
        1 aaaagtattt atcactggga tttttatgcc cttttgggtt tttgaatgga ggaaaatcac
       61 atgaaaatta ttaatattgg agttttagct catgttgatg caggaaaaac taccttaaca
      121 gaaagcttat tatataacag tggagcgatt acagaattag gaagcgtgga caaaggtaca
      181 acgaggacgg ataatacgct tttagaacgt cagagaggaa ttacaattca gacaggaata
      241 acctcttttc agtgggaaaa tacgaaggtg aacatcatag acacgccagg acatatggat
      301 ttcttagcag aagtatatcg ttcattatca gttttagatg gggcaattct actgatttct
      361 gcaaaagatg gcgtacaagc acaaactcgt atattatttc atgcacttag gaaaatgggg
      421 attcccacaa tcttttttat caataagatt gaccaaaatg gaattgattt atcaacggtt
      481 tatcaggata ttaaagagaa actttctgcc gaaattgtaa tcaaacagaa ggtagaactg
      541 tatcctaata tgtgtgtgac gaactttacc gaatctgaac aatgggatac ggtaatagag
      601 ggaaacgatg accttttaga gaaatatatg tccggtaaat cattagaagc attggaactc
      661 gaacaagagg aaagcataag atttcagaat tgttctctgt tccctcttta tcatggaagt
      721 gcaaaaagta atatagggat tgataacctt atagaagtta ttactaataa attttattca
      781 tcaacacatc gaggtccgtc tgaactttgc ggaaatgttt tcaaaattga atatacaaaa
      841 aaaagacaac gtcttgcata ttaagtagtt cagttttgga gtacaaaaag tgaaaatgta
      901 aacctaaatg agtatcaaat tgcaactgaa tttgatactc gttttttatt gttaaatcaa
      961 cgtttgttgt taatttacac cacaaaatgt aagttaaaat gtatataaaa atgcgaacgt
     1021 gaaataatta ttgtaggaat gcaaattggt gtgttaaaat ggttacaatt tactaagcaa
     1081 aaaggggtgc attttcctat gcaatttctt gagccaaaaa ataagaacgc taagtctgtg
     1141 gattgggaaa tatcagagca agtaagggtg atagttaagc aatacgctga atatgcagag
     1201 cgtactgaaa gcgaagcagt ggatgaattc ttattgaaca ttttagacga taagaagttt
     1261 attgaatgga ttgccaacaa gcgaagtaat aagcggattg ttgaaaaaat gggtatcaaa
     1321 gatagagtgg gttgatagac aatgaccaag cttaaaagat tagccacgaa ggaagatgaa
     1381 attgtagatg ttaaaattcc gatttcaaac gaagaattaa aagatagggc taaacaatat
     1441 gatttattaa ctccaaaacg atttgcaaca aggtataaca aaatgttgtt cttgccaacg
     1501 tcttttaaat ggaacggttc tgaatatccc attcaatata attactgcat caatccgttt
     1561 tgttgtaatt ttgggaaaga acagcacaaa ttcaaagatg taaaagggaa accaagtcga
     1621 tataaaatga ctggtagttc caaagataaa ggtcataaag gtatgtactg caatgacaat
     1681 cctattggca gaggtgtatc gcaaaactgt acagtaaccc ctttatctaa ttggtctgtt
     1741 gttgaagaaa ttaaacgcct tatagaaatc aatagcatcc aagatgttga gccagattat
     1801 caatttcata aagaaggctg ctcggaagaa gaatcaaccc cattcaatga gccgaagcaa
     1861 ttttacaaaa gggggaaaag taggggcaaa tctcaacgct atcaatgtaa ggcgtgtaag
     1921 aagttcacta atgttctacc aaaaagagaa gaaaccacta cttatcacca acaaaagaac
     1981 accattcttc caatgttcgc taagatggtt gttggaaggg tttcagtcag tcgtacttgt
     2041 gatattttag gaattggagt tggaacctac tatcataagc tggagtggct gtataggcgt
     2101 tgtttggagt ttttagaacg atatgagaca caaccccttc aaacaaagaa atttaacgag
     2161 atgtggctta atacggacaa gatgcactac tacctaaata acgtccgtaa gaaaggacaa
     2221 ggttcaaaga aatatacggg gtttgaagac cttaatatgc aaacgtatat agttgtatct
     2281 gctgaggtct tatcaagata tgtgttccgt tctgatgttg cctatgattg gaatatttca
     2341 atggatgaat taaatgaaga tacaagaaaa ttcaaagaag accatctcaa tacttttagt
     2401 cgtaaaaatg ataggttaga ttggtcttat tatccacaag aaccatccgc taacgatagc
     2461 gaaaatagga atgcttatct tcacgaatta gggaaaatta ctaataggag tagatttgtc
     2521 gatggtttaa atgttgatgc tccttatact acaactgctc actattggtt aattaaacaa
     2581 atggttaatg ccgacgaatg gcgaatgatt agtgatgatg acttctctat tagaaacgcc
     2641 ttttatagag tattcacgaa agaattaagg ttgagcgatg cacatcattt tatttgccaa
     2701 gttaacaaga cgaaaagcag aaaacaatgc ttaaaagaat tcggacaagc aaaagcagaa
     2761 ctgctcgatt ggggagatat cagaggattc aaaacaaagt ttctacggac tttggcatct
     2821 cattatctaa ctgaattact aacaagtcat caattccacg aagaggctat tagtaaagat
     2881 ggagaaagat atcgcaaata tgccgataac cctatcaaac atccattagc cactaaggat
     2941 aaaggatttt attcagttga ttgtagaacg gatttatctg ctctggaacc aaacgaaatc
     3001 gcaaagatgc tattaaatgt taatgaccat tcgaccaata gctttattca acagataaga
     3061 cgttatatat catctcttga aagaccatta acaacagctc gtggtgataa gaagagctat
     3121 atttacgcta acttcaatcc gaagtatgct caatttgcga ttacaattct aagaacttac
     3181 tacaactttt gtcgcccttt taaatcagcg gataaaaagg ttttgacacc tgctcaaaga
     3241 ttagggatta cagataaaca atttgattgg aaagatataa tttatttcaa atgacaaaaa
     3301 gagcttggag taatcccaag ctcttgtgct ttatctttac tgttcttctt caattaacgc
     3361 acccgttaac tcaataagtt ttttctttca tatacatgaa aatagtagtt atacggattt
     3421 ttatcattct ttatcccttt ttgggcaaat acctcattcc attcctcata attcacttct
     3481 ggaaaaaaag tatctccttc gaattcatga tgtatttttg tgatgtacat tttctcaaca
     3541 taagggaaaa acaaattata aatctgttct cctccgaaaa taaaaatttc ttcttcgttt
     3601 ttacataact caaaaacatc ttctattgaa tgaacaattt cacaaccatt aaaggtaaac
     3661 cccttatctc tcgtcagaat aatatttctt ctgtcaggta aggctcttcc gattgattca
     3721 aggttcttcc tacctaatat tatcggatgt ccctttgtag tatttttaac atattcccag
     3781 tccttgggaa tcctccaagg aatgtcattc tctttgccaa tcactctatt cttatccatc
     3841 gcagcaatca aagaaacttt catttataac acagcctttt taactaattt accatctaat
     3901 ttcttatttc tatcaaaaaa ccttttctca ttatcctgcc cgttagttga ataagcaatt
     3961 ttttctataa gttatattat cataatacca caaaatcaca aattttcatt caattatcat
     4021 tttcaattgc attttataaa tcattttgta tgtaatttgg tatgtaagtt aaaatgaaaa
     4081 aagtagtcca ttttgaagta cattttgtca ttttgtactc caaaactgaa ctacttactt
     4141 gcatatatac gcctttatag tggagtacta catttacgag attcggttag agtatcagaa
     4201 aaagaaaaaa taaaagttac agaaatgtat acttcaataa atggtgaatt atgtaagatt
     4261 gatagagctt attctggaga aattgttatt ttgcaaaatg agtttttgaa gttaaatagt
     4321 gttcttggag atacaaaact attgccacag agaaaaaaga ttgaaaatcc gcaccctcta
     4381 ctacaaacaa ctgttgaacc gagtaaacct gaacagagag aaatgttgct tgatgccctt
     4441 ttggaaatct cagatagtga tccgcttcta cgatattacg tggattctac gacacatgaa
     4501 attatacttt ctttcttagg gaaagtacaa atggaagtga ttagtgcact gttgcaagaa
     4561 aagtatcatg tggagataga actaaaagag cctacagtca tttatatgga gagaccgtta
     4621 aaaaatgcag aatataccat tcacatcgaa gtgccgccaa atcctttctg ggcttccatt
     4681 ggtttatctg tatcaccgct tccgttggga agtggaatgc agtatgagag ctcggtttct
     4741 cttggatact taaatcaatc atttcaaaat gcagttatgg aagggatacg ctatggttgc
     4801 gaacaaggat tatatggttg gaatgtgacg gattgtaaaa tctgttttaa gtacggttta
     4861 tactatagcc ctgttagtac tccagcagat tttcggatgc ttactcctat tgtactggag
     4921 caagccttta gaaaagctgg aacagaattg ttagagccat atcttagttt taaagtttat
     4981 gcaccacagg aatatctttc acgggcatat aacgatgctc ccaaatattg tgcaaatatc
     5041 gtaaatactc aactgaaaaa taatgaggtc attattattg gagaaattcc tgctcgatgt
     5101 attcaagatt atcgcaatga tttaactttt tttacaaatg ggcttagtgt ttgtttagca
     5161 gagctaaaag gatatcaggt taccactggc gaacctgttt gccagacccg tcgtctaaat
     5221 agtcggatag ataaagtaag atatatgttc aataaaataa cttagtgcgt tttatgttgt
     5281 tatataaata tggtttctta ttaaataaga tgaaatattc tttaatatag atttgaatta
     5341 aagtggaaag gaggagattg ttattataaa ctacaagtgg atattgtgtc ctatttgtgg
     5401 aaataaaaca agactacgaa tacgagtgga tactatactt aaaaatttcc ctttatactg
     5461 ccccaaatgt aagaacgaaa ctttaattaa tgttcaaaaa atgaatataa taacaatcaa
     5521 agagccagac gccaagacgc agagccgata atttgagaaa tgaaactctc atcttatcgg
     5581 ctctttttgt ttatctgaat tttactgact agccttcaat atttcttgct tcattgatac
     5641 cttttgctag tgcttccatt aaggatagtt ctttgtctgt aaagctatcc atgtattttt
     5701 ctacctgtaa ccttcgagtg cttttaatca aattatctgt aggtaagaaa aattcatcaa
     5761 tagatacatg gagtaatgat acaagatcat acagcacttg tgtacttggg tgttgtcctt
     5821 tgttttctat atttgttaaa taacgtgggt caatttcaat cattgttccc acttgttcac
     5881 gagtgagacc ccgtttcatt cgagcctctt taatcgctaa accaacggct ctaaaatcgt
     5941 atttatcttc ttttttacgc atactaaatc acctctatac attttactgt tcctatataa
     6001 ccataaacag gtatagaaaa acatgttata tagtttatga gttcctatag aacactgtgg
     6061 aatttattaa tgcgatgaat ccaatttcaa atagaaaggt cgctgatttt atttacctat
     6121 acaactttat aaaatttagt catactccta gaaagtagat aaaattggcg acttaatgga
     6181 gggaaaagtc gccatgagaa agataatctg tcatctagtt ctcttaatta agttcttatc
     6241 aaaaaaactt atccacctgt actggataga tatgaccatc aatgtggttt tgcagattac
     6301 atagatagaa aaatcaaagc atatgaacag agaaaacaat ctgtttatgt gcttttttgg
     6361 tcacttaata aatatctttt aggatttctc ttcatttatg caacaaatcc cccgttcgtg
     6421 tcggggttta gtatgaaggg agataaagaa gcacatgcca ctccctttca cgacagaaag
     6481 ggggtgaaaa gcatgaaacc atcttctttt caagaaacca tagaaaatca gtttgactat
     6541 atctgcaaac aagttattga agatgaacga aaagattact tcaaacatct agacagacta
     6601 tcagcaaaag aagtctcgtt ttctgatata gataactacc ttgtcaactc tttttctact
     6661 gttgatacgt acgtcactga tttccaacta tttaaattat ttggattaag aatcaatgtt
     6721 gaaagtgatt tattaagtga agccctgcgt catctttcag agaaaaaacg gaacattatt
     6781 ttacttcatt actttatgga tatgagtgat gtggagattg cggagttact aaatttgaat
     6841 cgttcgactg tttatcgtca tcgaataagt ggactagcca tgattaaaga attcatgaag
     6901 gagtgtaaag aatgaaaaca gcttatccaa gggttccctt ccccctcatt gtaaaggcga
     6961 cagatggcga cgtggaagca attaatcaga ttgttaaaca ttatcgaggg tatacctcaa
     7021 aacgttcatt acgtcgcatg acagacgaat atggaaattc tcatatggtt attgatgaaa
     7081 ccctacgtgg cagaatggaa acgaaactca tctctaagat tttatcgttc gaaattaaat
     7141 agtcccattt tttcgtggaa gcgtgtcgaa tcgtatccac gcttcccgat ttgggaggtt
     7201 tgttattcca tcaaagcata ttgtgtgttc agtaggtttt gataggctaa caagccaaac
     7261 gttctttgac aaccaaataa aggcaatcga atacgtttcg gtaagaaaag agctgatgaa
     7321 ctaacacgcc atgacctaca aaattagtga gcgattcatg ttagtaattc gaaagtaatc
     7381 tttagcagga ttgctgccat gactttctta tcgtgataat gatactcccg tatagccata
     7441 gtccgagcgt tagaagcgtc acaggcaatg ggtacggcta catgagaacc atgcagaagt
     7501 gaaattcctg tgagctttgc tagagctgtt cgattgctaa gatattcttc tcttgtcagt
     7561 gtattattat aaaactattt gatataatta taggtgaata ttggagtttt cctagcctat
     7621 aattgggaaa ggagtaataa tgataacggt cacaaaaaag gatttagttg agctaggata
     7681 tggaacttct ttttcagcag acatcataaa aaaagcaaaa gaattgatga ttacaaaagg
     7741 gcatatctac tatcaatctc gaaagctaga tagagtccct cgtgaagctg ttgaagaaat
     7801 tctaggaatt cggttttcaa atgaaaacag gtctgagtag tttttgcaca gtaaggagta
     7861 aaaactatgg ttaaagaccc aataaaaaaa gcaaagaatg gaacctatta ttttcgtgca
     7921 aatctaggtt atgattctaa agggaaaaaa attcaaaaat atcgtagtgg attcactact
     7981 aaaaaagaag caagagaaga atattcgaaa ctgctattga tgaaaccaga agaattaagt
     8041 gagaataagg ataaaatgac gtttaaacat tatatctttg aaattttcct tccatggtat
     8101 aagacacagg taaagctcag aacatatgaa aatcgcttac caactattaa gaagcatttc
     8161 tcttattttg ataaaatggc tgtttccgat attgagccta ttgatgtgca aaattggcaa
     8221 ctaaaattat caaagaagtg taagtcttct tatgtaagag ctgttcaagg actattttca
     8281 gttgctatgg atcgagcaat tgtccttggg ataaccacca caaatccatc taagattatt
     8341 ggcaatgtga aaaagcaaaa gtctaaaatt gaattttgga caaaagaaga atttgaaaaa
     8401 gtaatctctc taatttacaa agaagattac tatcaacact ttttatttat ctctctgtgg
     8461 tttttattta tgactggaat gcgaattggt gaagctactg ccattcaatg ggaagatatt
     8521 gattttgact caggagtact aaccatcaac aaaacactct attacaagaa tcaatcgaat
     8581 tatagatttg ttgaaccaaa aacaaaggcg agcgttcgtc atattgtatt agataaatgc
     8641 acattaactt atttatccga atggaaggaa gtacaacaaa atctaattca gacagacttt
     8701 gtaatgagct ataacggtat tcctacccaa aaacatacac tatcatatgc tattgaacgc
     8761 tatgctaagc tggctggtat ccatcgaatc agaatccatg ggttacggca ttctcatgct
     8821 tccttgttaa tcagtatggg agaaaatcca cttatcatta aagatcgtct aggacacgaa
     8881 gatattgaga cgactttagg aacgtatgga catctttatc ctaacagtaa tttcgaggta
     8941 gctaacaaat taaatggagc catttccttt aaagaagcaa aacaaaatta tgatacttca
     9001 ccaaagaatc aatttactgt agactacttg cgaaacaaag aaatgaaaaa agtgcaataa
     9061 aagtgcagta aaaagataag aagagccaga aacccttggt ataagaggat ttctagctct
     9121 cctacaacta ttcccactcc actgttgctg gtggtttaga agtgatgtca tacacgatac
     9181 ggttgacgtg ggctacttcg tttacgatac gtacagagat tttttgcaag acttcccatg
     9241 ggatacgggc aaagtcagcg gtcattccgt cgattgaagt tacagcacgg ataccgactg
     9301 tgtagtcgta agtacgtccg tcacccataa caccgacaga gcggatccct ggaagaacag
     9361 tgaaatattg ccagatatca cggtcaagtc cagcagctgc gatttcttca cgcaagatag
     9421 catcagattc gcgtacgatt tctagcttgt cttcagtgat ttcgccaaga acacgaatac
     9481 caagaccagg accagggaat ggctgacgcc aaacgattga atcaggcatg cctaactgag
     9541 tccctaattc acggacttcg tctttgaata atgtgtttaa tggttcgatc aattcaaatt
     9601 gcatatcttc tggcagtcca cctacattat ggtgagattt gatcgtctgt gctgtttctg
     9661 taccagattc gataacgtca gtatacaacg ttccttgtgc taggaaagag acgccttctt
     9721 caccagcaag ttttgttgct tcgtcatcaa aaacatatac gaattcattc ccaatgattt
     9781 tacgtttttg ttctgggtca gatacacctt tcaacttgtc taagaagcga tctcttgcat
     9841 cgactttgat gatgttcaag ccgaattttc cgcctagact ttccattact tgttccgctt
     9901 cgcctttgcg tagcagaccg tggtcaacaa agatagatgt caattggtca ccgatcgctt
     9961 tttgtaaaag aaccccaacg acactagaat caacgccacc tgaaagacca agcaagactt
    10021 ttttgtcacc aacttgttca cggatttttt ctacttgcat atcgataaag ttatccattg
    10081 tccagttacc ttcacagtga caaacatcaa aggcaaagtg gcgcagcaat tcgattccgt
    10141 attcggaatg acgtacttct gcatggaatt ggataccata aaaattacgt gttgtgtctt
    10201 ggatagaagc aatcggacaa tctttgcttg ttccgactgt ttcaaagcct tcaggaactt
    10261 tagttaccaa gtcaccatgg ctcatccata ctgtttgttg ttttggtgtt tctttaaaca
    10321 agacagcttg atcgcttgtt acttctaaca aggctttccc atattcgcgg tttttagcag
    10381 gctctacgga accgccaagt ttgtacgtga tcaattgcat accatagcaa atccctaata
    10441 caggaatacc taaattgaag atctcaggat cgatcccaaa tgcattctca tcatatacac
    10501 tgtttggtcc gccagaaaga atgattccct ttggattcat cgcttttact tcttctgcag
    10561 taatgcggtg gctcaataat tcggaaaaga ctccaagatc acgaatacgt cttgtgatca
    10621 attggttata ctgactaccg tagtctaata cgataatctt ttcaacgttc gtcatatcgg
    10681 caacgtttgt caaatttatt acccctatcc ctttaaattt tatctcaaaa tagtcaaact
    10741 attttaaaat gctaatattt tcgcatgaaa atcgaatcga tgatatgatt ttcagtttta
    10801 tggagaatta tatcggctct tcctcgtgta ggcaagatat attcctgtaa atttttcaaa
    10861 ttcacatttt tccatacatc ttttgccatt ttaaaggctt cttcacgatc tccgatagca
    10921 aaacgataat aataattctc tggattttgg aaagccgtat ccagtaacgc accaaaacga
    10981 tccaaatacc atttttcgat cagcacggga tcggcatcga catagacaga aaaatcaaaa
    11041 aagtcgctga cgtagatttg ctgattcgca ggtaattgaa gcgtattgat cccctcaacg
    11101 atcaagatat caggctgctg gatcaattcg tacttgtctg gaatgatatc atacacatca
    11161 tgtgagtaaa caggtacttt tatctcttcc tgaccagatt ttacacggtt caagaaatcg
    11221 atcagcatct ccatatcata actttcagga aatcctttac ggtccatgat tccacgctct
    11281 tttaatatac gatttggata aagaaatccg tcagtcgtga tcagctgaac gtttcttcgt
    11341 ttgaaggtac gagacaaaat cgtctgcagc agtctggctg ttgtactttt tccaacggct
    11401 acacttccgg cgatcccgat aataaaaggc ggtacttcta cgtatttatg aagaaataaa
    11461 cctttactca agaacaaaga ttcaaattct ttcatataaa gataaatcag atgagcaaga
    11521 ggtacataga tatctcgaac atcctgcatg gaaatgcggt cattcaaact tttgatatta
    11581 tctagttcat cttgtgtcaa aggtgcttgc ccattacgat aaaattctcg ccattcttct
    11641 cttgccacac ggtagtagtt cattggatca ttcatattag cctcctgcgt tctcccatca
    11701 atggaactat tttaacacaa aataaacgca aaccccacca cttaagagag attgccgatc
    11761 tttttgtata atctttataa tagaaagaaa cggagtctta ttcttgactt gcgcacggat
    11821 acataaaagg atagactgtt gtcatgaaag aaatgagaag gatggaagac atgacaaatc
    11881 attactattc tgaacgccca gatacggcgc acgattttga acaatgggct tttgagttaa
    11941 aaggcagaac ctttcaattt gtaacagatt ctggtgtatt ttcacgtgaa acagtggatt
    12001 atggttcacg tgtcctgatt gatgctttcg attgggaaga gttacctgat gaaggaaaga
    12061 tcttagatgt gggatgcggg tatggaccga ttgggttagc tttggcattt gcgacacagc
    12121 ggtttgttga aatggtcgac attaatgaac gagcggtaag tctagcgcaa ggaaacgcaa
    12181 agcgtaacca aatcgaacat gttgatatcc atcaatcgaa tatctatgaa gcagttcatg
    12241 aagaaaccta tgcagctatc gtaagcaatc cgccgatccg tgctggaaaa aaagttgtac
    12301 atgaaatttt gacaggcgca tatcctcggt tgaagaaggg cggtacactg acaattgtga
    12361 tccagaagaa acaaggagcg ccgagtgcgc aaaagaaaat ggaagaaaca tttggtaatg
    12421 cagaaatcgt cacaaaagat aaaggatatt atatcatcaa aagtgtgaaa gaataactag
    12481 ctgaaaaata gtggagcgac gaaagagtgg aactgggcgt gcatgtacgg gtacgtatca
    12541 gttctttttt tgttgagaag tttttaaaaa caaagaacta ttttttcttc aaaaagaaat
    12601 agtaggaaca gagtcgttgt tttttacgat aaaataaata agatacagaa cttgtataga
    12661 aggctgccaa gtcggtagat aaaggaggtt tttatcaaaa aacatacttt ccttttttct
    12721 ctattctttt cttgggggtt ttggtacact gatagggaat ttagatagaa gggagccaat
    12781 aggatgaagc tgaccatcag agatatcgca gaaatggcag gcgtatcagt aacaactgtc
    12841 tcacagattt tgaataacaa aggaagcaga tttagtgata agacaagaaa aaaagtgttg
    12901 tcgatcgtga atgaacatca ttataagccc gattatttcg cttcaaatgt gatcaatcga
    12961 cattcaaaga ccatcggaat gatcgtccct gatgtcactg atttcttctt ctccaaactg
    13021 atcgaaggtg tagagagcta tttgaatcct ttgggctatg tgatcatgtt atgcaattct
    13081 cgtcatagcc aagaaaatga gatcaagtat ttacaagagc tttcccaccg ctcagtggac
    13141 ggaatcttgc tggccacacc aaatatttta cctgagaaat atgcattaga cagcggtttt
    13201 tatcaaaaaa tgccaatcat tttaatcgat cgcggattga atcgtcggga taacggacgg
    13261 cttatcgtaa aggaatatga gggcgcctat caagctgttt cgctgtttat tgaaaatggc
    13321 catacgaaga tcggcatgct gaaggaaacc actggatact atcaattaga agaacgtttc
    13381 aacggatatc gtcatgcgct gaaagatcat ggcctgcctt ttaatgggaa atttgttgaa
    13441 catggagagt tgactgtcca aggtggatat actgcatcaa aaaaattact caaacataaa
    13501 gatatcacgg ctattttttg cgggaatgat gccatggcga tcggatgcta tcaggcgatc
    13561 gatgaattgg gcaaaaaaat cccagaagat atctcagtca taggattcga tggattaaag
    13621 ctgtcagaat acatgattcc gcaattgacg acagtgcagc agccaagttt tgatattgga
    13681 ttttatgctg cacgtttcct gatcgatacg atcgaatttc ctcaaagaaa agttccgaac
    13741 aaagtgttcg aaacaaaatt aattattcgt gagagcgtaa aagtattgac aaagggctaa
    13801 ctcggcggta tattgcttat gtaagcattt acaaaggcta gttagtgctt tttttatttt
    13861 tagtaaacca gtttactaaa gtggtttacc tttcctaaaa ataatgtata ataccgaatg
    13921 aagacaagga ggatcaggca atgcgaatgg tggatctgat tgaaaaaaag agggatggaa
    13981 acgagctgtc gaaagaggaa atcgagtaca tcgtcacgaa ctatacgaat ggaaagattc
    14041 cagattatca agtcagtgca ttattgatgg caatttttta tcaagacatg acaaatgaag
    14101 aaatcacgaa cttaacattg gcgattgcta attctggtga tgtcatcgat ttgagttctc
    14161 ttgaaggaat aaaggtagat aagcattcga caggtggcgt tggtgacacg accacattga
    14221 ttttagcccc cctagtagct agtgtcggag tgacagtggc taaaatgtct ggacgcggtc
    14281 taggttacac aggtggtaca ctagacaagc tagaagcaat tcccggattt cagatcgagc
    14341 tttcggatga agaatttgta cgcattgtta atgaaagtaa agtagcggta attggtcaat
    14401 caggaaactt ggcaccggcg gacaaaaaat tgtatgcctt gcgtgacgtc acagcgaccg
    14461 ttgattctct tccgctgatt gccagttcga ttatgagtaa aaaaatcgcg gcaggagcag
    14521 atgcaatcgt tttggatgtg accactggag acggtgcttt catgaagaac atcgaagatg
    14581 caagacgtct ggcaaagacc atgacgagca ttgggaaact ggctaaccga gaaacagtgg
    14641 cggttatctc agatatgtca gaacctcttg gcgaagctat cggaaatagt ctagaagttg
    14701 tcgaggcaat cgaaacatta caaggaaatg gtccagaaga tctagtagaa atgtgctacg
    14761 ccttaggaag ccaaatggtt gttcttgctg gcaaagctaa gacaaccgat gaagcaagaa
    14821 cgttgcttca agaagctctg gaatcaggta aagcgttagc aaaattcaaa gaaatgatcc
    14881 aaaatcaagg gggagaccca gcgatcgtgg aacatccaga acgaattttg actgcacgct
    14941 acacaatgga gcttcctgct aaacaaagcg gagttgtgtc aaaaatcgtg gcaaatgaat
    15001 taggaattgc tgcaatgatg ctaggtgctg ggcgtaaaac caaagaagaa gatattgatc
    15061 acgctgttgg attgaaactg cataaaaaaa ttggtgacac tgtgacaaaa ggtgagtcac
    15121 tattgacgat ctatagtaac gacgaagaaa tcacttctgt cattgaatta ttatataaaa
    15181 atattgaaat tggtgaatcg gcaatgaagc caacgttgat tcatgacatc atcacagaat
    15241 aaaggaaaaa agcggacttt accgttttaa agaaaagggg aaaagaaatg gaactaaacc
    15301 gaatgatcga ccatactatt ttgaaagcag acgcatctaa agaagctgtg atgcaaatta
    15361 tcgaagaagc aaaaaaatac catttttatt ctgtctgcat caatccagca tgggttgcgc
    15421 tagcgaaaga acaattacaa ggtgagccag tagccgtatg tacagtaatc gggtttcctc
    15481 taggagcaaa cacacccgag acaaaagcat ttgaaacaac agatgccatc aataacggtg
    15541 cagatgaagt cgatatggtc atcaatatcg gtgctttgaa atcaaaggat gatcaacaag
    15601 tccaaaaaga tatcgaagca gttgttgaag cagcaaaaga tcgtgcgctt gtcaaagtta
    15661 ttattgaaac atcgttatta gatagagaag aaatcatccg agcttgcgaa atagcaaaag
    15721 ctgcaggcgc tgattttgtc aaaacatcaa ctggtttctc aacaggcgga gcaaaggtag
    15781 aggatgtccg tttgatgcgt gaaactgtag gaccagaaat gggtgtcaaa gcttccggcg
    15841 gtatccataa cgaagaagaa gcaatggcaa tgatcgaagc tggtgctaca agaatcggaa
    15901 caagcgcagg agtagcaatc gtatctggtt caactggtga aggctattaa ttttttcaaa
    15961 ggagaagctc atggcagaac tcaaacaaga atggatcaac acagctattg aagcgttaga
    16021 taaagcctat gttccttatt cccattttcc ggtaggcgct tgtctcgtaa cggaatcagg
    16081 caagatttat caaggcatca acattgagaa tgcatcattt ggattaacga attgtgccga
    16141 gcgcacagct tttttcaaag cagtatctga aggagaaaaa acatttacgc atctagtcgt
    16201 agcggggcac acccccgatc ctatctctcc ttgtggtgca tgtcgacaag taatggcaga
    16261 attctgttcc cctgatatgc cggttacgct agtaggagac aatggtgtga cgaaggcaac
    16321 gaccgttcgt gcactgctgc cttatgcatt tactgaaaaa gatctataac tcatgttcat
    16381 tcagtcagct gactgttgaa aaataaaaca attattttag gaggctttac agaaatgaaa
    16441 aaagcaaaat tatttggttt aggtgctgtc gcactagcag cagggttatt tttaggtgca
    16501 tgcggcaaca acggctctac tgattcatcc ggtggcaaaa gctcatcaga cacaacaact
    16561 gcagcattga tcacagacac aggtggtgta gacgaccgtt cattcaacca gtcagcttgg
    16621 gaaggactag aaaaatgggg gaaagatcaa ggactttcac gaggaaatga tggcttccaa
    16681 tatttccaat cttcaaatga atcagattac atcccaaata ttgaccaagc attgaatgct
    16741 ggattcaaaa cgatttttgg tattggttat aaactaaaac cagcgatcca agaacaagca
    16801 acaaacaata caggtacgaa ctttgttatt atcgatgatg tcatcgatgg attagataac
    16861 gttgtatctg caactttcaa agacaacgaa gcttcttatc ttgcaggtgt tgcagcagct
    16921 tatacgactg aaacaaacgt agtcggcttt atcggcggtg taaaaggtga agtaatcgat
    16981 cgttttgatg ctggattcaa agccggtgtt gatgcaggtg cgaaagaatt aggaaaagaa
    17041 atcaaagtat tgaatcaata tgctggtgac ttctctgctc cagataaagg acgttcaatt
    17101 gctcaaggta tgtatgcaca aaacgcagat atcatcttcc atgcttcagg cggtacaggt
    17161 aacggtgtat tccaagaagc aaaatcattg aacgaatcag gcgacaaaaa agtttgggtt
    17221 atcggtgttg accgcgacca atcagacgaa ggcgaataca cattaaacgg cgaaaagaaa
    17281 aacttcacat tgacatctac actgaaagca gttggaacag tagtagaaga tttggcacaa
    17341 aaatcagcgg acggtaaatt ccctggcggc gaacacactg tttatggatt gaaagaagac
    17401 ggtgttggtc taacagaagg acaactttct gacgaagcga aaaaagcagt tgatgaagca
    17461 aaagaaaaaa tcatttcagg tgacgtaaaa gttccagaaa ctcctgaaga aaactaaaac
    17521 catttctata gccttgttag acacatgttt gacaaggcta tggtttgatt agaacaagaa
    17581 ttgttcattt atccaagctg ccttgtgaca gtttggataa gggaacaact ccctgaagta
    17641 atgtttgttt caaaagaaga acgctcacta agcgtgaatc ttagtcaatc gttgccctag
    17701 atagaaatcg ttaggatgtg aaaaaaaggt ggttcaagaa aatattgtca tagaaatgca
    17761 gaatattacc aaagaatttg gaacattcaa ggcaaatgac aacatcaatt tacaagtaaa
    17821 ggcaggagaa attcatgcgt tgcttggaga aaacggtgct ggcaaatcta cattgatgaa
    17881 cgtgctttcc ggattattag agccgacatc agggaaaatt ttgatgcgtg ggaaagaagt
    17941 aaagatcaca agtccgacca aagccaacca attagggatt gggatggtcc atcagcactt
    18001 tatgcttgtt gatgccttta ctgtaacaga aaacatcgtg ttgggaagcg agcctagtcg
    18061 cgcagggatg cttgaccata aaaaagcacg aaaagagatc caaaaagttt ctgaacagta
    18121 tggattgtca gtcaacccag atgcttacgt tcgtgatatt tcagttggaa tggaacaacg
    18181 ggtagaaatt ttaaaaacac tttaccgagg agcagatgta ctgatttttg atgagccgac
    18241 agctgtattg acccctcaag aaattgatga attaatcgtg atcatgaagg aattagtcaa
    18301 agaaggcaag tcgatcattt tgattacgca taagttagat gaaatcaaag cagtagctga
    18361 ccgctgtaca gttatccgcc gtggaaaagg aatcggtaca gtcaacgtta aagacgtaac
    18421 ctcacaacaa ttagccgata tgatggttgg aagaacggtt tcattcaaaa cgatgaaaaa
    18481 agaagcgaag cctcaagaag tcgttttgtc tattgaaaat ctagtggtga aagaaaatcg
    18541 tggattagaa gccgtgaaaa acttgaactt agaggttcgt gctggcgaag tacttggtat
    18601 cgctggaatc gatggaaacg ggcagtcgga gttgatccaa gctttgactg gtttgcgaaa
    18661 ggcagaaagc ggacatatca agctaaaagg ggaagacatc actaataaaa aaccccgaaa
    18721 aatcactgaa catggtgtag gacacgtgcc agaagaccgt cataaatacg ggttagtcct
    18781 agatatgaca ttatctgaaa atatcgcact acaaacctat catcaaaaac cttacagtaa
    18841 aaacggcatg ctgaattatt cagtaatgaa tgaacatgcc agagaattga tcgaagaata
    18901 tgatgttcga acaacgaatg aacttgttcc cgcaaaagct ttatcaggcg gaaatcagca
    18961 aaaagcaatt atcgctcgtg aagtcgaccg agatcctgat ctgttgattg ttgcaaatcc
    19021 aactcgtgga ctggatgtag gtgcgatcga atttattcat aaacgtctga tcgaacaaag
    19081 ggacaaatac aaagcagtgt tactgatcag tttcgaatta gaagaaattt taaatgtttc
    19141 ggatcgtatt gctgttatcc atgaaggaga aattgtcggt atcgttgatc cgaaagaaac
    19201 atctgaaaat gaattaggtc tattgatggc gggctatacc ttagaagaag cacgaaaaga
    19261 gttagaaaag ggggctggcg cacatgaatg atcgcagcga aaaattccgt aatttattag
    19321 tgcctgtact ttcagttgtt ttaggattga tcttaggcgc gatattgatg gctgcttttg
    19381 gttttcagcc catccaaggt tattcttcta tgctagatgc agcccttggt tcgcaaagag
    19441 ggataggtga aacgttaagg caagcaacgc cgttgatttt tacagcatta ggtttttctg
    19501 tagctaactc agctgggttt ttcaatattg ggctttctgg acaagcactt tgcgggtgga
    19561 tcgtcagtgt ctgggtggca cttgcgtttc cagatctgcc aaaagttgtt ttgcttccgt
    19621 tatgcgtgat cttaggtgcc ttagctggtg cgatggcagc ggctgttcca ggtttacttc
    19681 gagcattttt tggaacaagt gaagtgatcg taacaatcat gatgaactat atcttattat
    19741 acgtaagcac atttgcttta catgaagtga tgcctgaaag ctatcgctca agtttggact
    19801 cttccaatat gatcagtcaa aatgcctcac tgcggttacc atggttaaca caagcttttg
    19861 gcggctctcg cgtaaactca ggattatttt tagcaatcat cgcgcttatt ttcgtttggc
    19921 tgctgatgaa gaaaacgact ttaggatttg aaatccgttc tgttggattg aatccttttg
    19981 cttctgagta tgcaggaatg agtagtaaac gtacgatcgt tttgtcgatg gtcatttctg
    20041 gcgctttggc agggcttggc ggtgtggttg aaggattagg aacgtatcaa aacttctttg
    20101 ttcaaacgac ttcgctatct attggatttg acggaatggc tgtttccttg cttggttctg
    20161 gatcagcaat cggtattctt ttatcagcga tcttgttcag tattttgaag atcggtggac
    20221 ttggcatgca gacaggagca ggtgttccgt ttgagatcgt caatgtatcg attgctttga
    20281 ttatcttctt tgtcgggatc aacttcctga ttcgtttcgt catggctaaa gtcttccaag
    20341 ggaaaaaaca agaagagatc gttgcgacaa tagaatcgaa accaacgaat caaaaccatg
    20401 aagggggcga actataatgt cgactatcga attaattgcc ttgatcctta cttctacact
    20461 ggtttattca acaccgctaa ttctaacgtc actaggcgga actttttctg aacgaagcgg
    20521 gatcgtaaac gtagggctag agggaatcat ggtcatgggt gcttttagtt ccatcgtttt
    20581 caacttgacg atgagtccat cacttggttc tatgacacca tggatcgcta ttttagtggg
    20641 tggcgtcgta ggtatcctat tttctctgat tcacgcagta gcaacagtca gtttgcgtgc
    20701 tgaccatatt atcagcggga cagtaattaa tctgatggca cctgctttag gtgttttctt
    20761 gatcaaagca tggtatggta aaggtcaaac tgataacatc acgcagaatc tggggtattt
    20821 ttctttccca ggattagcag atattccggt cattggacca attttcttta aaaatacatt
    20881 cttacctgct tattgtgcta ttttgattgc tgtcctagct tgggtgatca tcttcaagac
    20941 gaaattcggc ttgcgcctcc gttcagttgg tgaaaatcct caagctgccg acacattagg
    21001 tatcaatgta tatgctatgc gttattcagg cgtactgatt tctggcttgt taggcggtat
    21061 gggcggtgct gtttttgctc agtcgatttc cggtaacttt tcagctggta cgatcgttgg
    21121 acaagggttc atctccatgg ctgctatgat ctttggtaag tggaatcctt taggggcaat
    21181 gggtgcctca ttgttctttg gttttgctca aagcttgagt atcattggcg cacaattacc
    21241 agttatttct tcgattccgc cagtattgct gcaaattgct ccatatttgc tgacaatcat
    21301 tgtgctggtt gttttcttag ggaaagcttc tggtccaaaa gcaaacggga agaactatat
    21361 caaatcaaaa taatctttta gcgaataaaa agggtgggtt acgggaagtg tttttcgtct
    21421 cgcccttttt gttttaaagg caataaaaat aaaaagaaag aaggaataat catgtttaaa
    21481 cgcgtacatc tgatcgtcat ggattcagta ggaattggag aagcaccaga tgccgataag
    21541 ttcggtgatg ttggttctga tacattagga cacattgcga aagaagcagg attgaacgtt
    21601 ccacatttag agcagcttgg tttaggaacg atccgtcctc ttgaaggtgt ggaatcagtg
    21661 aaagatcatc aaggctatgc gacgaaatta gaagaagttt ctgtagggaa agatacgatg
    21721 acaggccact gggaaatcat gggattgaat atcaaaactc catttcgtgt gtttcctaat
    21781 ggattccctg atgatctgat caaacaaatc gaagaatttt ctggccgtaa agtcgtatgt
    21841 aacaaacctt atagcggaac agctgttatt gacgattacg gtgaacacca aatgaaaaca
    21901 ggcgatttaa ttgtatatac ttctgccgat cctgtccttc agatcgcggc acatgaagaa
    21961 atcattcctt tagaagaatt atatcggatt tgccaattcg ttcgtgatat aacaaaagat
    22021 gatccgtata tgattggccg aatcattgca cgtccgtacg tgggtgaacc aggagacttc
    22081 acacgaacaa gcaatcgcca tgattatgcg ttgaatccat tcggacacac agtccttgat
    22141 tctttgaaag aagcaggaaa ggacgtgatt gctgttggaa agatcaacga tattttcaac
    22201 ggacaaggaa tcactgaatc tgtccgcacg aaaagcaata tggatggcgt tgaccagctg
    22261 ctgaaagtta tgaaaaacga tttttcagga atcagcttta ccaatttagt agattttgat
    22321 gcattatacg gtcatcgtcg ggatgtagtc ggctatgcgc atgcgattga agaatttgac
    22381 ttacgaattc cagaattgct tgaagcgatg gaagaagacg acttgctaat gattacagcc
    22441 gatcacggaa acgatccgac gtttactggt accgatcata cgcgagaata tgtaccgtta
    22501 ttggtttaca gtaaaaaaat gaaagaacaa ggggcattgc ctcaaggata ttattcagat
    22561 atttcagcaa cgattgctga gaactttagt gtttcttcga ctgaaaatgg cgaaagcttc
    22621 ttgaaattat taaaataatc tttttaaata ggagaaagca aatgaccaag ttaagcgaga
    22681 tgttacaaga aacgacccag tttatccttg ataaaggagt aaaagaaatt gattttggtt
    22741 tgattctagg ttcaggatta ggtgaattag cagatgaaat cgaagatgct gtaatgattt
    22801 cttatgatga gatcccgttc tttcctgttt cgacagtagt tgggcatgca ggaaagctag
    22861 tttatgggtc tctggcaggc aagaaagtgt tagccatgca agggagattc cactactatg
    22921 aaggtcattc catgcaaaca gtgacctatc cagtacgtgt gatggccgga ctaggggccc
    22981 attcagtcat tgtgactaat gcatgtggtg gtgtcaacga atcatttgta ccaggagatt
    23041 tgatgctgat taccgatcat atcaatttta ctggtgaaaa tcctttgatc ggtccgaatg
    23101 aagatgcaat gggccctaga tttccagata tgagtcaagc ttatacgcct gactatcaag
    23161 caacagcaaa agcagcagca gaaaaactag gtttggatct taaagaaggc gtatatatgg
    23221 gctattctgg accaacttat gaaacgcctg cggagatccg aatgtctcgg acaataggag
    23281 ccgatgcagt agggatgtcg actgttccag aagtcatcgt agcagctcac agcggattaa
    23341 aagtgttagg aatctcttgt attaccaact tagcagcagg tatgcaagcg aatctgagcc
    23401 atgaagaagt tgtagaaaca actcagagag tcaaacaatc ttttaaagca ttggttaaag
    23461 aaacattgac attattgtaa taggaggaac aataaaatga gtgtgcatat tgaagcgaaa
    23521 gaaggcgaga tcgccgataa gattttactt ccgggagatc ctttgcgagc taaatatatt
    23581 gcggagactt ttttggaaga tccagtttgt tacaatcagg tacgcggtat gttaggcttt
    23641 accggcaaat ataaaggaga acgtatttct gttcaaggaa caggaatggg aatgccatca
    23701 gccaccatct atgcccatga attgatccag tcgtatggtg taaagaaatt gattcgagta
    23761 ggtacatgcg gtgcattgtc aaaagacgtc catgttcgtg atttagtact agcacaaggt
    23821 gcagcgacca gttcttcgat gatcgaaaaa aatttccaag cctttcattt tcctccaatc
    23881 agtgatttca atctattact gaaagcatat gagatagcta aagaaaaggg ctatacggtt
    23941 catgtaggaa atgttctttc ggaagattct ttctacaaag atgatttgac agagacgttc
    24001 caattagctg aacttggcgt tttaggagtg gaaatggaag cagcggctct ttattactta
    24061 ggcgcgaaat accatgtgca gacattaagt ttgatgaccg ttagcgacca tttgatcacc
    24121 ggtgaagaaa caacagctgc agaacgtcaa tctacgttca atgaaatgat cgaagtagga
    24181 ttggaaacag cgatagcaga ataagaagga aatgaaaaag ctgccctgca ttaagtaata
    24241 agaacatcca agcaaaaata gctcctcatg tttttgactt ggctggactt attattgcaa
    24301 atgtaagctt ttgaacaccg tttaccaaga ggttgtgaat ctgccgttta actctgagtc
    24361 acaaggaaca atttcaaaaa acagcttttc atgtttgatg aatattgtga cttgtgaccg
    24421 agaagttggc aggagaaaac cgtttatacg aaaaaaggga ctgtgacaaa tcgtgtcaca
    24481 gtcccttttt tatagtgttt tttataggta gtatttttta gcaactgtta agtcatcatt
    24541 taattcataa acaagtggtt gacctgttgg aatttcaaga tccatgatgt cttcgtcaga
    24601 gataccttcg atatgtttag ctagggcgcg taatgagtta ccatgtgcag ctactaaaac
    24661 agttttgttg tctagtaatg ctggagcaat ttcatcttgc cagaatggca aagcgcgttc
    24721 taatgtcact ttcaagtttt ctccaccagg aatatcgcgt ttgtctaaca ttgcgtaacg
    24781 acggtcattt gctgcagaac cttcgtcagt ttcttccatt aatggaggaa gagtatcgta
    24841 agaacgacgc cagatgtgca cttgttcgtc gccgtatttt tcagctgttt cttttttgtt
    24901 caaaccttgt aatttaccat agtgacgttc gtttaagcgc catgatttga tttgtggtac
    24961 ccaaagttga tctgaatatt ctaagatcaa gttacatgtt ttgattgcac gagttaaaac
    25021 agaagtgtaa gcaacatcaa attcgattcc agcttctttg attttgcgtc cgccttcttt
    25081 tgcttcttcg attccttctg gtgctagatt tacatcagcc caaccagtaa attggttcaa
    25141 tgcattccat tcactaagac cgtgacgaga aaaaactaat tttggcatgt ttagctctcc
    25201 ttttaagata ggtttatatt tctgaaattt tcagttctgt tttattgtac aattttttgg
    25261 aagagatttc catcgaaaaa ttagataaga ttctatgaga aaacaagtag agaatataaa
    25321 aaaacgaaca gtgcccacgc aactgttcgt ttttaggtat tagatgcata attgctcgat
    25381 ttctttttca ggtaaattga tatatttata acatgttcca actgaaacac cgcattttga
    25441 agaaataaat tggatcgttt cttttttttc ataatagaga cgtcggattt ttttgatcgt
    25501 ttttgcatcg atttttggtc ttccgccgat ttttcctttt ttacgagctt catctaatcc
    25561 aactaatgta cgttctttga tcaatgcgca ctccatgttt gctaggtttt ccattaattg
    25621 gaaatagatt tttcccattt cgcgcgtatc gattccttca tcaagactaa tcaagtgcag
    25681 cccgcgatct ttgaataatt gcgtgagttc tgttaattgc cgcgtagatt tgcctaatcg
    25741 atgaagttgg caaatgacta gtgcatcgcc ttgttgcatg tcagcgatca ctgactccaa
    25801 atgaacagca gtagaatcat tttgttccac ttcatatgtc tcatggaaaa tttcttgaca
    25861 accaaagtca gataattgat caacctgagt tgttaaatca acgtcattta tggttgtgcg
    25921 tgcataacca ataattttca tggtgattcc tttctagcag tgattttcta actggatata
    25981 acagtaaaaa tgtagcataa agtacgtcaa aaagatagtt ttttcgcaaa ttttccgctg
    26041 tcttttttta ttggatgatc aagcagtttt tctttattta cgacggattg cgtcaagaat
    26101 ctcaacgcct ttttctgagc cgacaactac tttagaaacc atgtttagga aaagcccatg
    26161 ttctactacc ccaacaagtt catctaaata ggtagctaag ctttctggct gttcgatgac
    26221 ttctagatgc agatcaatga tgtaatgacc accatctgtg gttaagactt ctccgttttc
    26281 gtctgtacgc aagacgggtt ggaatctttt ttcttcaaaa atatgaagta gctgttggga
    26341 gccataaggg atcacttcaa ctgggagtgg gaacttccca agtttatcga ctaatttaga
    26401 agaatcaacg atccaaatcg tttcttttga gtacgtagcg actattttct cgaaaagcaa
    26461 agcagcgccg ccgcctttga tcccttgaaa atcagcactg atttcatcag caccgtctat
    26521 tgtcaaatcg actacgggga cttcatcaat actctttaac ggtattccta atttttccgc
    26581 ttgttctttt gtcgcatttg aagtagtaac tcctgtgata cgtaaacctt cttcacgcat
    26641 acggcgacca atctcttcta ccatataata ggcggttgaa ccggtcccga gacctacgat
    26701 catcccgtcc tttacgaatt tcgcggcttc aatgccagct aattgtttta aattcatctt
    26761 tgcgtccacc tttttttagt ttgagcatcg tgctttcctc actattttag cgtgatttac
    26821 ggtaaaaaga taccattctt ttttattcca ataaagcttt ttcaaaaaaa attgcatctc
    26881 ttgttgacat gttttttcat tcgtgatatt ctaacaaagg tgctttatct acaggtttct
    26941 gtgaagaaac gtgctgactg tttatcaaag catatttgat aaggatgcag acattgcatt
    27001 tattttttat cacaaaaaaa gaaccacctg gatgtgtgga actagacgca aatcgtggga
    27061 aggaggaaac aaggaatgcc tacaattaac caattagtac gtaaacctcg taaatcaaag
    27121 gtggaaaaat ctaattcacc tgctttgaac aaaggatata acagctttaa gaaaactcaa
    27181 actaacgtaa actcacctca aaaacgtggg gtagcaactc gtgtgggtac aatgacacct
    27241 aaaaaaccga actcagcttt acgtaaatat gcacgtgttc gtttgtcaaa cttaattgaa
    27301 gttactgctt atatcccagg gattggtcat aacttacaag aacacagtgt tgtattatta
    27361 cgcggtggtc gtgtaaaaga cttaccaggg gtacgttacc atatcgttcg cggtgcttta
    27421 gatactgctg gtgttaacga ccgtaaacaa agccgttcta aatacggaac taaaaaacct
    27481 aaagcttaat tttaattatt aaacaaatga aacttgcttc tactatatag aagtaattac
    27541 tatgaacaat atttcggaag gaggagttac ggatgccacg taaaggtcct gttgcaaaac
    27601 gtgatgtttt accagatcct atttataact caaaattagt aactcgcttg atcaaccgtg
    27661 taatggttga cggaaaacgc gggattgcag ctaacattat ctataatgcc tttgacttga
    27721 tcaaagaatc tacaggtaac gatccattag aagtgtttga acaagcaatg aaaaacgttc
    27781 aacctgttct tgaagttaaa gcacgtcgtg ttgggggttc aaactaccaa gtaccagttg
    27841 aagttcgccc tgaacgccgc gttactctag gtcttcgttg ggtagtaaac tacgctcgct
    27901 tgcgcggtga acacacaatg gaagaacgct tagcaaaaga aatcatggat gctgcaaaca
    27961 acacaggtgc ttctgttaaa aaacgtgaag acactcataa aatggctgat gccaaccgtg
    28021 cgtttgctca ctatcgctgg taagattctc tctgtttggt cggcttttat aagtcgacct
    28081 cagatagtgt ccttattttt agcaactaga tagtaaagag aggagaaaca aggagaatga
    28141 ccagagaatt ttctttacaa aacactcgta atatcggtat catggcccac atcgatgctg
    28201 gtaaaacgac tgctactgaa cgtatcttat actatactgg taaaatccat aaaattggtg
    28261 aaactcatga aggagcttca caaatggact ggatggaaca agaacaagaa cgtggtatta
    28321 cgatcacttc tgctgctact acagcacaat ggaagggtca ccgaatcaac atcatcgaca
    28381 caccaggaca cgtagacttc acagttgaag ttgaacgttc attgcgtgta cttgatggtg
    28441 ctgtaactgt tcttgatgca caatcaggtg ttgaacctca aactgaaaca gtttggcgtc
    28501 aagcaacaac ttatggtgta cctcgtatcg tatttgcaaa caaaatggac aagatcggtg
    28561 ctgacttctt atattcagta tcaactttac atgaccgttt gcaagcaaac gctcacccaa
    28621 tccaattacc aattggtgcg gaagacgact tcacaggaat tatcgacctt gtaacaatga
    28681 aggctgaaat gtatacaaac gatcttggaa cagagatcga agaaacagaa attcctgaag
    28741 aataccgcga attagctgaa gaatggcgcg aaaaattggt tgaagctgta gctgaaactg
    28801 atgaagaact tactttgaaa tacttagaag gtgaagaaat cactgaagct gagttaaaag
    28861 aaggtatccg ccgcgcaaca gttaacgtag aattctaccc agtactatgt ggttcagcat
    28921 ttaagaacaa aggggttcaa ttactattgg acgctgttct tgattacttg ccatcaccgt
    28981 tagatatccc agctatcaaa gggattgacc ctaaaactga tgaagaagta gaacgtcctg
    29041 cagacgacag cgctcctttc tcagctttag catttaaagt tatgactgac ccattcgtag
    29101 gtcgtttaac tttcttccgt gtgtactctg gtgttttaca atcaggttca tacgtacaaa
    29161 acgcaacaaa aggcaaacgt gaacgtgttg gtcgtatcct acagatgcat gccaactctc
    29221 gttcagaaat ctctgaagtt tacgctggag atatcgctgc tgctgttggt ttgaaagaca
    29281 caactactgg ggacacacta tgtgatgaaa agagtctagt tattcttgaa tccatggaat
    29341 tcccagaacc agttatccaa gtcgctattg aacctaaatc aaaagctgac caagataaaa
    29401 tgggtgtggc tttacaaaaa ctatctgaag aagatcctac tttccgtgct gaaacaaatg
    29461 tggaaacagg cgaaacaatc atcgctggta tgggtgaact tcacttagat atcatcgttg
    29521 accgtatgcg tcgtgaattc aaagttgaag ctaacgtagg tgcgcctcaa gtatcttacc
    29581 gtgaaacttt ccgtgctggc actcaagccg aaggtaagtt tgtacgtcag tctggtggta
    29641 aaggtcaata cggtcacgta tggattgaat ttacaccaaa tgaagaaggt gctggtttcg
    29701 aatttgaaaa tgcgatcgtc ggtggggttg ttcctcgtga atacattcca gctgttgaaa
    29761 ctggtttgaa agatgctatg gaaaatggtg tcttagctgg ctatccatta gtagatatca
    29821 aagctaaact ttacgatggt tcataccatg atgtcgattc aaatgaaact gccttccgtg
    29881 ttgctgcttc tatggcatta cgtgcagcag ctaagaaagc taatccagta atccttgaac
    29941 caatcatggc tgtagaagtt gttatccctg aagattattt aggtgatgtt atgggtcacg
    30001 taactgctcg tcgtggacgt gttgaaggta tggaagctcg cgctggcggc caacaagttg
    30061 ttcgtgcgat ggttccactt gctgaaatgt ttggttatgc tacaactctt cgttctgcta
    30121 ctcaaggtcg tggtacattt acaatgacat ttgaccacta cgaagatgta ccaaaatcag
    30181 tacaagaaga aatcattaag aaaaacggcg gaaaagctga ataatttctt aaaaccggat
    30241 agtcctgcaa agagattatc caaagagact atatttttaa tatagtttcg tgtaaaataa
    30301 ttatcgatga tatgggcatg tggtgtacct tgtgtacatc atatacctat cagacaaata
    30361 aaaaatatat tgtattttta ggaggacatt ttaaaatggc aaaagaaaaa tttgaccgtt
    30421 ctaaaccaca cgttaacatt ggtactatcg gacacgttga ccatggtaaa actacattaa
    30481 cagctgcaat cacaactgta ctatctaaga aaaacggcgg ccaagctatg gcttacgatc
    30541 aaatcgatgg tgctccagaa gaacgcgaac gcggaatcac aatctcaaca gctcacgttg
    30601 agtatgaaac agatacacgc cactacgctc acgttgactg cccaggacac gcggattacg
    30661 ttaaaaacat gatcactggt gctgctcaaa tggacggagc tatcttggta gtttctgctg
    30721 ctgacggccc aatgcctcaa actcgtgaac acatcctatt gtctcgtcaa gttggtgttc
    30781 cttacatcgt tgtattcttg aacaaagtag acatggttga tgacgaagaa ttactagaat
    30841 tagttgaaat ggaagttcgt gacctattaa cagaatacga attccctggt gacgatgttc
    30901 ctgtagttgc tggatcagct ttgaaagctc tagaaggcga cgcttcatac gaagaaaaaa
    30961 ttcttgaatt aatggctgca gttgacgaat acatcccaac tccagaacgt gacaacgaca
    31021 aaccattcat gatgccagtt gaagacgtgt tctcaattac tggacgtggt actgttgcta
    31081 caggtcgtgt tgaacgtgga caagttcgcg ttggtgacga agttgaagtt gttggtattg
    31141 ctgaagaaac ttcaaaaaca acagttactg gtgttgaaat gttccgtaaa ttgttagact
    31201 acgctgaagc tggagacaac attggtgctt tactacgtgg tgttgcacgt gaagacatcc
    31261 aacgtggaca agttttagct aaaccaggta caatcacacc tcatacaaaa ttctctgcag
    31321 aagtatacgt gttgacaaaa gaagaaggtg gacgtcatac tccattcttc actaactacc
    31381 gtccacaatt ctacttccgt acaactgacg taacaggtgt tgttgaatta ccagaaggaa
    31441 ctgaaatggt tatgcctggc gacaacgtaa caatggaagt tgaattaatc cacccaatcg
    31501 ctatcgaaaa cggtactaaa ttctcaatcc gtgaaggcgg acgtacagtt ggtgccggtg
    31561 tcgttacaga aatcaaagct taattcactt aagttttaaa gtaaagcaac atttattcgg
    31621 acgtaatcgc tcggaacgaa ggtcttagga tctttgttcc gagctttttt atttattgca
    31681 ggatcaggaa aaaagacagg agtattataa atagaagaaa cacgaaa
//
LOCUS       DAIGOK010000018        31372 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.018, whole genome shotgun sequence.
ACCESSION   DAIGOK010000018 DAIGOK010000000
VERSION     DAIGOK010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.018"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(188..2269)
                     /gene="cadA"
                     /locus_tag="NOA01_000639"
     CDS             complement(188..2269)
                     /gene="cadA"
                     /locus_tag="NOA01_000639"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HCI0631862.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(2793..3152)
                     /locus_tag="NOA01_000640"
     CDS             complement(2793..3152)
                     /locus_tag="NOA01_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCI0631863.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(3182..3523)
                     /locus_tag="NOA01_000641"
     CDS             complement(3182..3523)
                     /locus_tag="NOA01_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631864.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            complement(3520..4194)
                     /locus_tag="NOA01_000642"
     CDS             complement(3520..4194)
                     /locus_tag="NOA01_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HCI0631865.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(5247..6545)
                     /gene="asnS"
                     /locus_tag="NOA01_000643"
     CDS             complement(5247..6545)
                     /gene="asnS"
                     /locus_tag="NOA01_000643"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCI0631866.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(6585..7775)
                     /locus_tag="NOA01_000644"
     CDS             complement(6585..7775)
                     /locus_tag="NOA01_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCI0631867.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(7796..8323)
                     /locus_tag="NOA01_000645"
     CDS             complement(7796..8323)
                     /locus_tag="NOA01_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HCI0631868.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(8370..11159)
                     /locus_tag="NOA01_000646"
     CDS             complement(8370..11159)
                     /locus_tag="NOA01_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCI0631869.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(11306..11506)
                     /locus_tag="NOA01_000647"
     CDS             complement(11306..11506)
                     /locus_tag="NOA01_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCI0631870.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(11959..12279)
                     /locus_tag="NOA01_000648"
     CDS             complement(11959..12279)
                     /locus_tag="NOA01_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631871.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(12782..13900)
                     /locus_tag="NOA01_000649"
     CDS             complement(12782..13900)
                     /locus_tag="NOA01_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631872.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            14371..15678
                     /locus_tag="NOA01_000650"
     CDS             14371..15678
                     /locus_tag="NOA01_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HCI0631873.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            15799..16998
                     /locus_tag="NOA01_000651"
     CDS             15799..16998
                     /locus_tag="NOA01_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCI0631874.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNVFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(17025..18293)
                     /locus_tag="NOA01_000652"
     CDS             complement(17025..18293)
                     /locus_tag="NOA01_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HCI0631875.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLFYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPILQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(18457..19104)
                     /locus_tag="NOA01_000653"
     CDS             complement(18457..19104)
                     /locus_tag="NOA01_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HCI0631876.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKKER"
     gene            complement(19513..20016)
                     /locus_tag="NOA01_000654"
     CDS             complement(19513..20016)
                     /locus_tag="NOA01_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCI0631877.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(20139..20903)
                     /locus_tag="NOA01_000655"
     CDS             complement(20139..20903)
                     /locus_tag="NOA01_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HCI0631878.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            21011..21286
                     /locus_tag="NOA01_000656"
     CDS             21011..21286
                     /locus_tag="NOA01_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCI0631879.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            21951..22901
                     /gene="yidC"
                     /locus_tag="NOA01_000657"
     CDS             21951..22901
                     /gene="yidC"
                     /locus_tag="NOA01_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCI0631880.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPADSKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            23005..24321
                     /locus_tag="NOA01_000658"
                     /pseudo
     CDS             23005..24321
                     /locus_tag="NOA01_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012008915.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(25022..25222)
                     /locus_tag="NOA01_000659"
     CDS             complement(25022..25222)
                     /locus_tag="NOA01_000659"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631881.1"
                     /translation="MAEMHDHLLHSICERTGLSEEEVNQKLMEISEAMNKGVDFSMNQ
                     LDDFLAQHMKDALASDAADSVE"
     gene            complement(25572..25919)
                     /locus_tag="NOA01_000660"
     CDS             complement(25572..25919)
                     /locus_tag="NOA01_000660"
                     /inference="COORDINATES: protein motif:HMM:NF019886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCI0631882.1"
                     /translation="MDHLHDHDAENRLESNYARYLGGIEQTIEDSYTQDCMMSYTPDI
                     VEKIAPTDFKKMERKRLQEEIQKLTFNQRKVIEHYYFLNKNQQQIARDLRMSHAEITK
                     TLKRAFAVMKEVY"
     gene            complement(26314..26691)
                     /locus_tag="NOA01_000661"
     CDS             complement(26314..26691)
                     /locus_tag="NOA01_000661"
                     /inference="COORDINATES: protein motif:HMM:NF025104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631883.1"
                     /translation="MFFLAFLYLYIANVKYFSKWPLLLFWIISCVLLLVKNAVFQFLL
                     KKIGSKSHTRVLVVENGNLAKEYGLAMLSDPDADANIIGYVKDRKKEDILLEEPGFAN
                     YGDGLAKLKYFGRISDVQDVIVK"
     gene            complement(26715..27038)
                     /locus_tag="NOA01_000662"
     CDS             complement(26715..27038)
                     /locus_tag="NOA01_000662"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631884.1"
                     /translation="MIDRRDDSDRCWSIRYYYKGRINEIIKERFWNTVFDCIGDAASI
                     FVAYLLAMFICYHVLEAAPGINMLSVPYLMMAALYSILIADFLRIFRATNKTYFRKYQ
                     NGFLK"
     gene            complement(27100..27576)
                     /locus_tag="NOA01_000663"
     CDS             complement(27100..27576)
                     /locus_tag="NOA01_000663"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631885.1"
                     /translation="MSQIKFLVMDVDGTLTDGKIYMGECGEIMKAFNIKDGCAIHDIV
                     RAHNIEPIIITGRRSKILENRCKELGINILYQGIDNKIEKLLEITSDLSTVAYIGDDI
                     NDLSCMHPVKSAGGIVACPHDAVKKVLEISDFIAEHDGGNGAVREFVEWLVDTQNN"
     gene            complement(27593..27958)
                     /locus_tag="NOA01_000664"
     CDS             complement(27593..27958)
                     /locus_tag="NOA01_000664"
                     /inference="COORDINATES: protein motif:HMM:TIGR00022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCI0631886.1"
                     /translation="MELEEGRYDLSNGIYLNVESYDIYRFEERMYESHLKYLDIQCIL
                     LGCEYIVVESISNLQIEVDYSADKDIVFYSNSIVGNCVLLEGGKLLLLTPEDAHMPCI
                     RVNESAKVKKAVFKISINY"
     gene            complement(28041..29006)
                     /locus_tag="NOA01_000665"
     CDS             complement(28041..29006)
                     /locus_tag="NOA01_000665"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HCI0631887.1"
                     /translation="MDKIIEAKRVFDIEIEALKRTRDCLGKTFVRILDAIVNCEGKVI
                     ITGMGKPGHIAAKLAATFASLGTPAFRLHPAEAMHGDLGMVSEKDIVIAISYSGESDE
                     IVKILPNIKMIGATLIGITGNSKSTLATASDIVQILPEFEEACYLGLAPTSSATSVLC
                     YGDALAVVASGIYGFKDSDFGKFHPAGSLGKKLILKVSDLMSHGNEIPMVNSGTLLMD
                     AISVMSNKGLGIVSVIDQDGTLKGIITDGDLRRIIERKVDIYSVEIDDVMIANPKVFT
                     KDKLAVDALRFLKANSINILPIIDDEGMLVGTITWQQIVKAGIVA"
     gene            complement(29115..29891)
                     /gene="kdsA"
                     /locus_tag="NOA01_000666"
     CDS             complement(29115..29891)
                     /gene="kdsA"
                     /locus_tag="NOA01_000666"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010388312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCI0631888.1"
                     /translation="MNRLMLIAGPCVIESEENVMLIAKTIKEITEKLDIDYYFKASFD
                     KANRTSITSYRGPGIEEGLKILKKVKETYGLKICTDIHEPWQAEKAAEVCDILQIPAF
                     LCRQTDLLVAAAKTGKIINVKKAQFLAPWDMKNVINKLVEAGNSNIMLCERGTSFGYN
                     TLVVDMTSIVEMKKFGYPVVMDATHSVQKPGGKGDVTGGNRNNVEPLAKAAVATGADA
                     LFFEVHPDPDNALSDGPNMVLLDEFEGLLERVLKVYNAVR"
     gene            complement(29893..30630)
                     /gene="kdsB"
                     /locus_tag="NOA01_000667"
     CDS             complement(29893..30630)
                     /gene="kdsB"
                     /locus_tag="NOA01_000667"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCI0631889.1"
                     /translation="MKTIAIIPSRYGSSRFPGKPLAMISSKPMIQWVYENVSKAAMLD
                     AVYVATDDKRIFDCVESFGGMALMTSDKHTCGTDRLAECAEILQLRDEDLVLNIQGDE
                     PLIKAEMIADLYSCFDNDEVYMGTLKKHIEVQEELDNPNVVKVINDVNDYAIYFSRFC
                     VPYERDGKNSLHFKHIGVYGYKTWFLKKYSTMPKTPLEVSESLEQLRVLESGFKIRVK
                     ETKYQTVGVDTPEQIQQVEDELRKEGL"
     gene            complement(30627..>31372)
                     /locus_tag="NOA01_000668"
     CDS             complement(30627..>31372)
                     /locus_tag="NOA01_000668"
                     /inference="COORDINATES: protein
                     motif:HMM:NF024832.1,HMM:NF026018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HCI0631890.1"
                     /translation="LGIFSTDVQVGLYSQPEKIVKLLMTVITSLGAVMLPHIAVAVKE
                     NNIDRIRKEVLNAVSFVFCIGAPMVVGAIIIANTFIPWFLGDGYAKSVSLFQLLSPLI
                     LIIGFASVVGQAVLIPMKRQRVYTFSIMTGAVINLVVNLALIPHLEAIGAAIGTIFAE
                     LTVTAIQFYEVRNCIELKMASIITNVKNYLIGALIMGGVGGYVSSKLPNSVISTFITV
                     ILCILLYSILLICMKDKLLMDYLKRRKNR"
BASE COUNT         9223 a         6755 c         5395 g         9999 t
ORIGIN      
        1 tattttttcc atcaaaaaaa taagttattt tcttttattt catttacctt attgaatttc
       61 aagctatttt gaattttctg caaaattaaa aacattagtt attttataat cctatctatt
      121 ttctttacat ttttcattaa gaaaaggata taaaaaaaag aggacgttca ttcgtcctct
      181 tcattgatca acgtttattt tttacatgca agcttcgcat ggcattcaaa acagctaata
      241 ctgtcacccc aacatctgca aatactgcat ctcccattga tgcaaaacca aaagcaccta
      301 aggcaagtac caaaattttt atcccaatag caaagacaat gttctgtttg acgattttca
      361 atgttttctt tgctaaatgg attgcttctg caatcttcga tggttgatca ttcatgatga
      421 ctacatctgc tgcttcaatt gcagcatcgc ttcccaatcc acccattgcg attcctacat
      481 ctgctcttgc tagcaccggt gcatcgttga ttccatctcc gacaaatgcg gtctttttat
      541 tgtttctttg taagatttcc tctaaccgct gcactttatc ttctggaagc aattcgctat
      601 atatcttgtc cacgcctact tgcttcccga tatgatcagc aatttttttc gaatcgcccg
      661 tcaacatgac tgtgttcttc acaccagcct tttttaattg aaccaatgcg tctacggcat
      721 cttctttcaa ggtatctgca ataacaagat aaccactgta actttgatcg atcgctagat
      781 aaagcaatgt accgatttct tgacttgaag gaaaagagat ttcaaatctt tccatcaatt
      841 tgtgattacc tgctaatact tgtttgccat caacttcagc taagatccct tcacctgcaa
      901 attcttggat attagctgtt ggtaaaagcg gacctgcgta gccttctaaa acagattgag
      961 caatcggatg tgtagaaaac tgttctgcac tcgcaacata ttgcatgaat atctttggat
     1021 cgatttttgt atcgatcgtc tggatcgaaa agtcaccttt cgttaatgtg ccggttttat
     1081 cgaataccac tgtctcagtt tgtgcaagca actccaagta atttgaacct tttactaata
     1141 caccgatttt acttgctcct ccgataccgc caaagaaact aaggggtacg gagatcacta
     1201 atgcacatgg acatgagatc actaaaaatg tcaatgctcg atagatccat tcagaaaacg
     1261 ccgctccaga aacaacaagt ggaggtaaaa cagctaataa aacggccaat cctacaacga
     1321 ctggtgtgta atagcgcgcg aatttagtaa tgaaattttc agctggtgct tttttactgc
     1381 tggcattttc tactaattct aataatttgc tgatagtaga attttcataa gatttttcta
     1441 cttgaactaa aaatggttta tttttattga taaatccact taatactgtt tcaccaacat
     1501 atacagattt agggactgat tctcctgtaa gtgctgacgt atcgactaat gattctcctt
     1561 cgatgatcgt tccatctaac ggtacacgtt caccaggctt cacaagtaca aactgaccga
     1621 tctgcacgtc ttcagggttc acggtcatca atccttccgt cgtctgcacg tgagctgttt
     1681 ctggacggat cgccatcaat tcgcgaatcg attttcttga atgactgact gccaatcctt
     1741 ggaaatactc gccgatttga taaaatagca tcactgctac ggcttctgga tattcaccga
     1801 tcaacatagc gccaatcgta gcaacagaca taagaaaatt ctcatcaaag atttttcctt
     1861 tgacgatatt catcactgcc ttcttcacta catcatatcc tatcagcaga taaatcaata
     1921 aataccctat caagcgaatt ggttcattaa aatcaaataa tagcaataaa gcgatacctg
     1981 caagggaaaa taaaatccgg aacagcgtat ttctctcttt ttcatttcca tgttcagata
     2041 ctttttcctt atctatttct gttactttga catctggttc taattccttc actacttttt
     2101 ttatttcttg ttcaatcgta gccaaatgat cttcctctgc gtcaatcgtt agttttccag
     2161 aagtgaagtt taccgttgct tccttgactc cattgatctt ctgaactcct ttttcgattt
     2221 tcattgcaca gttcgcacaa tctaaacctt ctaatctgta acttttcatt ttaatgacct
     2281 cctcgcctca acatatgaat atctcttcat aactaattat aaatgaacgg ttattcatat
     2341 gtcaaggtat ccgaaagaaa aaatgaaaaa catctgcatc ggaaaatttc ttccatacag
     2401 atgttttggc ttattctttc tttttctttt cgatcattgc cactgctctt atccaacccg
     2461 cacttgcctt cttgatcttg acaggatagc aactaagttt gaagcctgtt gctggcaatt
     2521 tgtctaaatt cattaatttt tcgataggac aatatctttt ttcaatccct gcaaaatgtc
     2581 cttcccagat attcgaagct ccactacgct ttcaaaaata tagaaagagt tctttaatga
     2641 aaattatttc aactttccat aaacagtaac cccatataaa taaaagacgt aaaagtcaca
     2701 tggaagaact tatacaggag aacgcataga gtcaggtgaa atacatgtat cccacctgac
     2761 tctatgcttt atctaacctt tattattctc ttttatacaa tttctgcacc gaatggcatc
     2821 aacgccaatc gagcaagttt gaaagattgc ttggcaaaag gtatccctat gatcgtgata
     2881 catagaagga tgccgctgac gacatgtgcg agagcaattg gtaggcctga aacgatcagc
     2941 cagatgatat taacaatcaa tgacatgcca gactggtgat aaattacttc tttgccaaaa
     3001 ggcgcaaaag aaagggaggc gatcttaaaa cattgcatac cgattgggat acctatgatc
     3061 gtgatacacc aaagtatccc cgctgctaac cagctaagac caccgattag tccgccgaat
     3121 ataaaccaga tgacatttcc taaacaactc attgtatttc tccttctatt actgtttttg
     3181 attaaaactg ggcaaaaaat gattttattc ttcttttttt agcttccagt atcaaatatt
     3241 ctgcaaattt tttttggatt tcttccgtat tttcttttgt tactaatgtg tgttggatta
     3301 gttttcctga tggtcgctga tccaatcgtt cttctgactg ttgcacgtcc gcttgcgcaa
     3361 atccgataat ccgtttttct tttgttacag caacgagcaa tagtttattc ttatcattta
     3421 ggtcagctgc cgtttttgct tttacgaatg attctggatg atcagtaatc agctgattga
     3481 tttgaggtag atcttctttt actgcccacc gtatttccat catttctcac ttttctttcg
     3541 ctcattttta tctccactaa aaaaatcgac tgcggcaatt gcatctatcc agctttgtgc
     3601 aatgatctca tgtccgccaa tcgttgggtg gaccccatct tctcttgtat atttcgaaaa
     3661 accataaaca atccctaaag cattgaatat accatctaaa ggaaccagca atgcgccata
     3721 ttccttagca agtttacgaa caatttggat tttagggtct aagtctttac gccagccatg
     3781 acggtcgtcg gttataggaa gtacaaacgg ctccatcaag atgatgttgc agattcccac
     3841 tgtttgaagt tcatttagta attttcgata aacggattca aattgctgag cagatttctc
     3901 agttccgaat atagcctgat cgctagtatt atgccaggta tcattgattc cgatcaaaat
     3961 cgatacatag tcaggttgta aagctaaaca atcttcttgc cagcgtactt gcagatctgc
     4021 aattttgtct cctcctatcc cacggttcat gcattcaaat tccatcgttg gatattggga
     4081 gattagtttt ccggcaatca aaagtggata acctttacct agatcagttg cttctgctcg
     4141 gtctcttcct gcgtctgtaa tgctatcacc gataaataat atacgttttt tcatttgtca
     4201 aaaactcctt tttgaataaa acgatgattt tcttccttat attatcccat aagccctatc
     4261 aagacgaaag agaaaatata tgtataaaaa atgacaccag cctagtccct tcaaacaatt
     4321 gaatgaacgc tcatcaagtg tctagttttt tatgctattt ttttcttaac gtgccggatt
     4381 ccccttgatt ccttcgttgt ttggtccaga gagatcgatc acacctaagc ttccgtcaat
     4441 cagaccactg atgacgccat cagaacatat atttataggc tcatctaaga cttctagtaa
     4501 atgtcccttt actaatatct cttctagatt gcttgggata gattggtcat aactttatct
     4561 ggtccgatga tttcaagggt gaattgaact tacatatgga ttattgcatg ctgaaaatac
     4621 ggacctactc catcttcgaa tgttactatt agtttgtttt caggtgctag atgctgcttt
     4681 atttcaacag cagaagcatc atcaaatcag atttccattt gaattacctt ctttgtaaat
     4741 gtttgattga ttgcgatttc gttatagaac cttgtgaatc aaatagcaat ttcttttgct
     4801 ttcttctctt gtctgctgga aacttccttt atcccgtatt ttaaaaaaag aactgcgaga
     4861 gttcgcagtt cttttttaga ataaacggtg ctctccggcc aactcctcgg tcacaagtca
     4921 caatagtcat caaacatgaa aaactgtttt ttgaaattac tccttgtgac tcggagctga
     4981 acggcaggcg cacaacctct tccttaaacg gtgttcaaaa gccagtttct cggtattaag
     5041 ccaaatttat gcaaaaatgt ggaaagccat ttccacaaaa tttttcttaa tactcgaaac
     5101 taaacagctt tttcacaacc tctccactaa acggtattca aaagcttaca tctgcggtaa
     5161 taagtcccgc caattcaaaa acatgaacag ctgtttttgc ttggctgttc taattacttg
     5221 atgcagaaca gctttttcat cacctcttat ggataaattc ggttcaacaa acgtgggaat
     5281 ggaatagctt cacgtacatg gtcgatacca gcaatccaag tgactgttcg ttcaagacct
     5341 agaccaaaac cagagtgcgg aacagaaccg tatttacgta gatcaagata ccatgaatat
     5401 tctttttcat ccaaaccgtg attacggatt tgttctagca agaaatcata gtctgttgcc
     5461 cgttcgcttc cgccaataat ttcaccgtaa ccttctggtg cgatcatatc tgcacagatc
     5521 accacgtctt ctcttgttgg atgtggtttc atgtagaatg gcttgatggc ttttggatag
     5581 tttaggataa agacaggctg ttcgaatgaa ttcgcaatga acgtttcatg aggtgaacca
     5641 aagtcttcgc cccattcgat gtcatcaaat ccatttttct tcagtaattc gatcgcatca
     5701 tcataagtaa tacgagggaa tggcaatttt gtataagttt ttaagacttc tttgtctcgt
     5761 cctagtacat ctaatgcata ttcgcaatga tctaaaacat tttgtactaa gaatgctaca
     5821 tattgttcct gtacttctaa actttcttct tgatgagtga aagccatttc tggttcgatc
     5881 atccagaatt cgatcaaatg acgtcgtgtt tttgattttt cagcacggaa agtcggaccg
     5941 aatgaaaaga cttttccgaa agccatagca gcagcttcca tgtataattg gccactttga
     6001 gataaataag ctttttgatc aaagtaattt gtttcgaata gatccgtagt gccttctggt
     6061 gcagaaccag tcaagatcgg cggatcaatt ttgacaaaat gatgttgatt aaagaattca
     6121 tacgttgcac gaatgatttc atttctgatc tgcataatcg catgttgtct tgaagaacgc
     6181 agccataaat gacgatgatc catcaagaag tctgttccgt gttcttttgg tgtgatcgga
     6241 taatcgtggc tctcgcttac cacttccaca tttgtaattc cgatttcata accaaatttt
     6301 gaacgactgt cttcacggat ttctccagtt actaacacgg atgtttcttg gtgtagattt
     6361 ttagctgttt ggaaaacttc ttccgacact tcgcttttta caactacacc ttggaaaaag
     6421 gctgttccgt ctcttaattg taaaaatgcg atttttccgc ttgatcgctt atttgcgacc
     6481 caagcgccga ttgttactgt ttctcccaca tggtttttcg aatcgatgat ttgaatttgt
     6541 tccacgtatg actctccttt tatattcctt gaatttcttt aggattaatt ttgactttta
     6601 ctctcaataa attgcttgat tctgcgtacc gcttcttcta acgtagcgcg atcagtagca
     6661 taactcatgc ggacattttc tggtgcccca aagccttcgc cagtcacaag cgctacccct
     6721 gtttcttcta aaagatcttc tacccattgt gtgacatttt catatttaca catgcgtagc
     6781 gtttctttta cattaggaaa aagataaaaa gctccttgtg gcttttcaag agaaacaccc
     6841 ggcagttctg ccacaagcga atataacgta ttcaggcgtt cttcaaaggc gttccgcatt
     6901 tcttccactg tatcttgctc gcctttcaat gcttcaacag ctgcatattg actgactgct
     6961 gtcggattac tggttgactg ggaagcaaca gcgatcattc cattgatgat cacaggattt
     7021 cccaccgcat aaccaatgcg ccagcctgtc atcgcatatg ttttagacac gccgttgatg
     7081 atgatagtct gatttttgat tgcttctgaa atcgttgcaa ttggagtaaa ctcattgcca
     7141 ttataaacta aacgaccata aatatcatca gcaactatca aaatatcatt ttttactgcc
     7201 cattcaccga ttgcctgcag ttcattcttg ctataaatca ttccagtcgg attcgatgga
     7261 gaattgatga ttacggcttt tgttttttct gaacgtgctt gttctagctg ttcaacagta
     7321 actttccagc tattggattc ttcccctttc acaaagacag gaagtccttc tgccagtttt
     7381 acttgctcac cgtaactgac ccagtaaggc acaggaatga tgacttcatc ttgcgggtca
     7441 aggatcgttt ggaaaagggt atacaaagca aatttcgctc cgtctgttac gactgtttgt
     7501 gaaggttcat agacaagtcc atagtttttc ttcagataat caacgattgc ctgtcgaagc
     7561 tctgggattc ctgctgttgg tgtataatag ctagctttcc cattgcggat tgcttcgatt
     7621 gctgcttctt ggatgttttc tggtgtcgca aaatccggtt ctcccacagt caaactcagc
     7681 acatctctgc cttttgcttt taatgccttg gcctttgccg cagctgccaa agtgacagac
     7741 ggttctaatc gttttgttct ttttgataac tccatcccat tcattccttt accttttaaa
     7801 ttcccttgat tgtcttgact tcatctccag attcaaagcc aatcaggtaa taattcagtt
     7861 tcccatttgt ttcttttcct actacttccc acacaggctg atcatcatac atccctaaag
     7921 cagctttttc gatttggatt tcaggatggg ccgcagcaat tgtctgtttt gcttcgtttt
     7981 cagtcagtcc ctttgtttga tccaatactt ttactttttc accagattta ggaatgataa
     8041 cggcgatttc ttttccttta tcatttgtac ctgtcagtga aaagtatgtc ttctttcgag
     8101 taaaccaata aaaatgatcg gtttcctcaa gatttgcata tttctttgcg atttctactg
     8161 cttctttctt cgcctgcacc gccggacgac tggaacgcaa gtaaaaaatc gagctgatca
     8221 cgatgaccgc taataaaaat acgatcaagc caagtaacac gcgattaatc gttttttcct
     8281 ttgtctgttt tttcttcaag ggtcgtcgct cctttatctt cattcctttt cattatagca
     8341 gattttaatt atgaaaaaag acagctcctc taacttcctt cttcattttt ctttaagaat
     8401 tcatggatat cctgatagat ttcctccata gctagctctt tgatcggtac ttttttcggt
     8461 aatgcatgga tgatccgtgc gccatatttt gcagtgacta gtcttcgatc caaaataagc
     8521 aatacgccac gatctgtctc cgtccttatc aagcgaccta atgcttggcg taacttcaaa
     8581 gctgctttag gtacggattc ctggtaaaaa ggattgatcc cctctgattc taaataagcg
     8641 ttcctagctt tgaccatcgg gcgaagcgga ttatcgaagg gtaaacgtgt gactacaacg
     8701 atctgcaaag catctccagg caaatctaca ccttcccaaa agctgtcagc gccaaacaat
     8761 acgctttttt ctgacaaaat gaagcgtttc aaaagcttct cccggcttcc accaaaccct
     8821 tgagctagta tttctcgtcc tttttctagc atagacagat gcattttctg atagaccttc
     8881 tgcaaaacat catgagaagt gaataacaca agtattggcc gatcttcttg ttcgatcaga
     8941 cctgtaagtg ctgtctctaa ataatcgata tatgcttgat tgctgacttg atggacggca
     9001 ggtgcctctt ttggcacata gatccttgcc tgtttctcat aatcataagg agaggcaatc
     9061 actttaagag gtgctgcttc aatgccaagc ctcttagaga gatagtgtcg attagggccg
     9121 atcttcagcg ttcctccaat ataaagaata cgatcatatc gaggatacca aacagtcgac
     9181 ggcaacatag ccgcttgaaa atcatggaga tgaaaaatca tctgctgcgc tgtgttccca
     9241 ggaacgaacc aatggatcaa atgatctcgc cagtcttcca gccattgatc catcagcaag
     9301 tattgcttgt ctaattgatc aaataggcta tataaacttg ctaaatcgat ccgcatctgg
     9361 tttgtccagc tttcttggat aatctcaaaa gtcagacgca tttgtgactg tagctgctgg
     9421 atctcctgat aaaaaagctg caggtgatca atggctgtcc cagattccag tggcagctga
     9481 tccaacatct cttttgtcat catgacttct tctggatact gtccgttagg ttgcttgccg
     9541 ctcactaaac tcattaatcc ttctgccata caaatctgtg cttcgcatag cgcagaaagt
     9601 tcttctcgat aaatcgataa ccagcgtaag gcttcatgtt ctttcggtaa atagctttgt
     9661 attcggtcaa acagctgatt tggttcttct aattgctgga tttgtttatg gaaatagtta
     9721 atacttaatc gtttgtcatt tactttttca gcgatttccg gcaaatgatg tacttcatcg
     9781 atcaacaaaa atggactagc tggaagcaat ggttcttttc gctgtgtttc ttgtactaaa
     9841 aacgcatgat tgacaatcaa aaaattactt tgcctgattt tcttttgcaa gaaacgaaca
     9901 aagtcttgtt cgtaaaatga ctgatttctt gctaaaaatc caatacctct gtgcgcgata
     9961 tctgcaaaga atgaatggtt caaatttgtt aaatgtaatt catccaaatc accagtcttc
    10021 gtttgcgtca gccaaactaa gaccgccatt tgatacaaag catattgttt ttgttctggc
    10081 ggttccacaa gtgtgccttt aaaacgctgc aaatcaatat agtggcgata gcttttgata
    10141 attacaggtt gtatgggctg atctagcaat tgattcagca atggaatgtc tttttctatg
    10201 atctgctgct gcaaaactag cgaaacggta ctgatgacag cgggtttttc aggtgtagcc
    10261 aaataactca atggcagcag ataaccgatt gttttaccca ttcctgtagc tgcttcgatc
    10321 atcaaatctt ttgattcatc ttttgtgaaa tggtcgtaga ccgcattcat cagtctgttc
    10381 tgttcttttc gatacatcag tgttttacca aacatttttt cttttgcttg tttttttctt
    10441 ggatatgtcc gctcgccata atggacggat gtgaatagtt ccacttcttt tttctgcaaa
    10501 gctagcccat cgacgatttc taatgaggga tcaagtggct ctggtttttc tttcatttct
    10561 tcaatcacat gataaatgaa acgactcgta tccatacctg tgtgtctact taatcgcgca
    10621 atctgctcta acgtgatgat tggcaatttt ctcatccgtt cttcgatcag caaaagaagt
    10681 tgacctgtca cttcggcatc actatctgcc tgatgaggat tctcatgaat aaatccaagg
    10741 ctttctgaca aatcagctaa gcgaaagctt ggttccgtcg gcaagaaaat ttgtgcaagt
    10801 tctactgtat cgatgcctgg tatcttcaaa ctaggaagtc cgcatcgcat gagttcatgg
    10861 ttcaaaaaat tataatcaaa ataaatatta tgcgcgacaa atatggtgtc tgccaaaaga
    10921 ttgtaaatcg tttgggcgac atcttcgaaa taaggcgctt tttggacgcg ttgattcgtg
    10981 atccctgtta aatgctggat ttgttttgaa atcttccgcc ctggatgaat atcgatcgaa
    11041 aaacgtgaag tgatccgacc tgattcaatc atgacgcagc caaattggat gatccgatct
    11101 tcttttggat tcgtcccagt cgtttcgata tcaacgactg cataagttct tggtttcaaa
    11161 atatcttcct cttctcctgc ctctaatgtt cttttgaaat tatactacaa agtgcagaaa
    11221 aatactctca tcgcttttta aagaaaatag acaaaaacgc ccagacacgc aaatgtctgg
    11281 gcgtttattt tttagtatag gttaattaag ctttagtaac gtttgttgct tgaggtccgc
    11341 gttggccttc ttcaacatcg aaagttactg cttggccttc ttctaaagtt ttgaatccgt
    11401 cgccttggat agctgagaaa tgaacgaata catcgtttcc gttttctgca gtgatgaatc
    11461 caaatccttt ttctgagtta aaccatttta ctgtacctgt ttccataata aaaatcctcc
    11521 ttgtgtatca acacatattt tgcaattact tgaatggtgt atatctggaa gatgtttatt
    11581 tgaaacaagc taccacagat taccgaacaa aaactactcc tatagtatat cataccgttt
    11641 acaaaaaagc tagagttttt cgactttttt tattaaaaaa agttttctca gcgattacat
    11701 aaagtgaaaa accgaacttt tttttaaaat aaacgacata ttgttcagtt ggtgtttttt
    11761 tgctctgatg ttctttttta aactaaaaaa attattcttt ctgactactt atcggttctg
    11821 tttcaacggt cgaggctggg gaagattctt tagtaggtct gatgttctca tggtatttct
    11881 gatagcctat gacagttacc agaagaacaa ggcaaaccaa tacgaccaac gagattagtt
    11941 tttctttttt gctcatatct atacctcata aataacagga tgatgtccat catatttctg
    12001 agtatgagta aaagcaaaag ctgcagctgt agaatgtaag aaatgccagc aactttcata
    12061 atcacaggtc ggacagtacc aaaagtaacc aaatcttggc tgcaaagacc gttcttctgt
    12121 ttcaggaatc gcagaatcaa gatctggtgt ctgtacataa acatttttgt acagacgtgt
    12181 gcctgtaata ggaaaccgct gtgtttcttc agcaaaaact ggctgacttg ttaaacttag
    12241 ggatagagac agcaaacacc ataacagtct ttttttcata aagacctcct tatcgataag
    12301 tttttcccaa ttaaaatcat atctcaagaa cgcccgttat tcacattttg ttcataaatg
    12361 atgttttttg tatcataatc gtcaaaaaga ggtcgtgacc acgtcacaac ctctttttta
    12421 aacggtgttc cagaagtagc ttcttcggaa ataagccgaa atttcacaaa aatttgagaa
    12481 ataatttttg tcaatctctt cttatttctt gaagctgacc acttctgtca caacctcttc
    12541 cttaaacggt gttcaaaagc ttgcatctgc ggtaataagc ccaccaaaca aaaaatatga
    12601 agagctattt ttgcttgctg ttcttattac ttgatgcaga acagcttttt cacaacctct
    12661 catacacggt gtttaatcag ctgaccttcg gtattagctc aaaaaacggc aaaatatgtg
    12721 aaactatttt tccgtttgtt tctctaatac tcaggtcaaa acactgattt cacaacctct
    12781 tttaataacg aagaatcgct gtcagactct tttcatctgg cgcatctttt tgatccaaaa
    12841 taaacacttt tccgccatta atgatcacat catctgctaa tgcattcaac acttgacgcc
    12901 gatcatattc tgtttcagga tcttcaccga aaccatcagc taagtttgac gtagcgataa
    12961 ataaatgtga cacttttcct tctttcgcag ctggaacgat atcaactaac tgatcgatga
    13021 atttcttatc taagagttta ttgtattctt ttgattcttg agctgcgagt tcacttgaca
    13081 gcttcttcgc cccttcttcg atatctctaa aggatagctg cgcaggagaa gcagaaatcg
    13141 caacgtctgc acaataatag ggatttttag caattttttt gaacatcgtc tgattttctg
    13201 gtagagcaaa taaataaatc ggccattgtt ctggattttc gaaagaatcc ttcaagtacg
    13261 tatcaactgc ttggtaatag ttgacccaat cgatctctac ttcttcatct tttggattta
    13321 cgccatggaa agttactcca tctcgggctc cgcctcctcc attagaagag aagttcaaac
    13381 cgccacctgt cagttcagat cccaatgcag taacgacatc aaccggtgcc ccttctggta
    13441 gagatacttc ttttacttgt ttgttgctga cttcatacaa tttcatggag tcgcggttca
    13501 atcccaataa ataataagaa tagttgaact gcccattttt gataatagcc agtaaataag
    13561 gaagatcact tacatagtat tgatcgtcta ccgggatact caaacgatgg atataggttt
    13621 ggtcagcagt tataatgacc gctacactcg tagttgcact acgccaaaac gaaggatctc
    13681 ctaacaaatt ttcaattttt tcttggaacg gggaccagtc atgttcggaa tattttttct
    13741 caaaacgttt ctttgcttct ttcgcaaaat ttttgaattt gatctgatct ttttccacat
    13801 cttgatgggc gacatgcgta ttcagcatga tcgtgacgaa tggtccttta gcgccttctg
    13861 aagtcaattg tgacaataat tctttatcct tttttgccat aaacgtaccc ctcctaaaaa
    13921 aatcattcct ttgtttctat ctaaagtcta ccacaacact atttttccat acaataaaac
    13981 gtatctaaaa aaataaaacg gtttctacgg ttttaaattt agctattttt ttaagttatc
    14041 ttcataattt cctcaattat ttagccccag tcttcaacta ttcttcatga aatgctgata
    14101 aactaagaaa aagaataaaa gaggtggatc ttatgcattt gattatgatt ttcagtttat
    14161 taagtattat cagtttattt gctttcagcg cttcccgctt attagatgcg gaaacacagc
    14221 ctattcctgt aaaagtacgt aaaaacgacc gctaaccttc cataacgtgc gttcttatcc
    14281 taaagttttt tcagaaacat tgaaactctc tcccctctct gataaaattt taatttgtag
    14341 cgaaacaaat taattacaag gagatcggat atgtccataa aaaaaacacg tctagtccta
    14401 ttattggcta gtctcattct ttgccttgct tcttttctac cagctgttgc tgtgcaagct
    14461 gaagatactt tcaaagtaaa tgcaaaagca gcctttgctg tagatgcgca aagcggtaag
    14521 attctttatg atcaagatgg agaaaaaacc atggggatcg cttcaatcac aaaaattatc
    14581 ggtctttaca tcgtccttga tcaagtaaaa gaagggaaac tatcatggga tgataaagtt
    14641 tcaatttcag attatgcaga aaatttgagt gtcaccccag atttatcaaa tgttcctttg
    14701 cataaagaaa acacctacac cgttaaagaa ttattcgact ctgcaatcat ccagtcagcc
    14761 aacgcttcaa tggttgcctt ggcagaaaaa atctctggca gcgaagcaaa attcactgaa
    14821 cgaatgaaag aacaattgaa agattggggt atcaaagatg caacgatcgt taatgcatca
    14881 ggactgaata actcttatct tggcgaaaac cgaccagaag gtactggaga aaacgacgaa
    14941 aatcaaatgt ctgctcaaga tgtggctatc gttgcccgtc atttgatctt ggattttcca
    15001 gaaatactgg atgtatctag cactacgaca caaatgtttg gcgaaaacac gcaatcacca
    15061 gtagaaatgg tcaactggaa ctggatgctc ccaggattcg tgaattataa agaaggcgtg
    15121 gacggattaa aaactggtac gactgaactt gcaggagcct gtttcgtcgg tacgatcact
    15181 aaaaacgatc aacgaatcat cactgtcgta ttgaatgcag acggccatgc ggaaaaccca
    15241 agtgcccgtt tcaatgaaac agctcgctta atggattatt gctacgacaa ttggtcagaa
    15301 aaagagcttg gaaaagccaa tgcaagtatc cctagtctaa aaaccattga tgtaaaagac
    15361 ggaaaagaat caacagtgcc cgttgtgctt aaaagtcctg taaaagtatg ggtacggaac
    15421 gatatggata ctggaaaatt aacaatcaca ccaactcttg acaaaaaaca agtcactgac
    15481 aatgagatcc aagcacctat ggataaagga acaaaaatcg gtaccgcaac gatcaccttg
    15541 gcagaagata aattaggtta tctagaagac aacaacgcgc catccacaga aatcatcacg
    15601 gataaaaccg tagaaaaagc aaatattttc gttattggct ggcgacgcgt cgcagaattt
    15661 ttcggtaact tgttttaatt gaacaaaaag aaaaaagcat ctttccatgt ggaagatgtt
    15721 tttttgcttt ttctagttta aataacagct tttttcatct attttcgtta tactgtataa
    15781 gaaaaaggag ttgaatttat gaatctaacc agagatctga tttttttggt tctctatctt
    15841 attggggtat tctccatcgt ttattggcga aaacagaaaa aggatccttc cttgttcttt
    15901 tacagcagta cgtggacatt tgcggtaatc ttgacttatt ttgtcgtgtt ggaaattttt
    15961 tattcgacct tttatttttt gattccctta gtgtttttca gtatctttgc cttttcctat
    16021 ttttcagaaa agcgaaggct gctgaatggt cttttattca atgttttcct cattagtttt
    16081 ggtatctact tgtttgtctt gctatacgaa acacaaaata tttttcttgg cggactgatt
    16141 gccttgatca cgatcccgtt actgcttgtc tttctattcg gcatttatgg attgatcgtt
    16201 tttctttttt ggaacggtgt cacagtttta cgtagagagt cgcattcatt agctaatctt
    16261 ttaacgttga tactagccat ttttcttaca ttgtttcttg tttttgactt tttcttgtta
    16321 aaatatttac cacaatggat aaatgctttg tttttctgca tcccattgat cttgatctat
    16381 ctatttatcg tattctacaa ctttttgact gtctcattct tgtatcagtt caaccgccct
    16441 cgatacaacc aagacttcat cgttgtgtta ggcgccgggt tgatcaatgg agaaacagtt
    16501 tctcctcttc ttgccaagcg aatcaataag gcgattgctt tttattgggc acaaagccgt
    16561 gcgacattaa atccgcctat cctattaatg tcgggtggac agggagcaga cgaaaaagta
    16621 ccagaagcaa tggcgatgaa acaatatgcc atggaacaag gaattcctga aagagatatt
    16681 cttgtcgaaa ccaattcgac aaccactttg gagaatatgc gctattcaaa ggaaatcatg
    16741 gatcagcaaa tgaaagggcc ctatcgcgcg atcttcagtt ccaacaatta ccacattttc
    16801 agagctggtc tctatgcacg acaagcgaaa ctgaaggcaa atggcattgg ggcaaaaaca
    16861 gctttctatt acttgcctaa cgcgttttta agagaataca tcgccatctt gatgatgaac
    16921 aaaaaaagac atatgatcat ttgcggattg cttttcgcag gttccgcatt cttatcactg
    16981 atcactttac ttttctaaaa aaccaaggtg tgacaaaaca tatgtcacac cttgtttttt
    17041 tcttttattc tcttgacgat tggcaagaat agtattcctt ttttgacggc tacttgctgt
    17101 gcagaataaa taccgctgtt tcctgaacac ataaagctga tcaagcagat cataaagaag
    17161 taaacagcag cttgtgaacc aaataattcg attcccatca caaaacaggc aatcggtgta
    17221 tttgtcgcac cagaaaatac cccgataaaa cctaatccag caagaaaagg aacagaaaca
    17281 tggagcaaag gcgctagtac tgcgccaagc gttgcaccaa tctcaaatag tggtgtcact
    17341 tctccccctt ggtaccccgc acctaaagac aagaccgtga agaataattt cccaataaaa
    17401 tcataagcat gtgcctgtcc attaaatgat tcctgcagta tcggaagact caacccaagg
    17461 taacgttgtg tttgcaaaat caacgctgcg gctacaacta ctgctccacc tatcgtattt
    17521 cgccaaacag gatgaggaat ccattgcgca taccgcgctt ttaaaaagac gatcgaacgg
    17581 ctgaataccc aaccaatcaa accaaaagca atcccagcaa caaaaacttt gaagaacagc
    17641 aacatactcc attcaggaat cacacccatt tggtaatgta gatgagaaac accatagctt
    17701 tctgtcatga aatttgcaaa aagcgcagca aagaagctag gaaagattgc ttctgttcgg
    17761 actttaccga ttgccaacac ttctaatcca aataatgttc cagctaaagg tgtgccaaat
    17821 acagaactaa aacccgcact gattccactg ataaccaaaa tctcccgttc tgctttatcc
    17881 aatcggaata aatgcgctat attatcagct aatgctcccc ccatttgaac tgctgttcct
    17941 tcacgaccaa cagatccccc aaataaatgg gtagtgatgg tcccaaacaa ggtcaatgga
    18001 atgagccgca aagggatctt ctcttctcct ccattccctt gatcgatcac taagttattc
    18061 ccacgactag aaagaccgcc atgatacgca taaagataag caaaaagcgc gccgctgact
    18121 ggcaatagat aaaatagcca aggatgtgct agtcggatat ctgtcacgaa cgtcaggcta
    18181 tttaagaaaa aagcagacaa gctccccatg aatactccga gaatcgctgt gaccaccagc
    18241 cacttcaaca tgtacatcaa aatacgcact ggactcggta attgtttttc cataaaaaat
    18301 tcctcctcaa ataaaaaaac gcctactaaa gaaaggggct ggccccagac tttagtaggc
    18361 gtcattagct atcttacgtt agcatttaaa ggcgaacacc atcacctatt cacttccatt
    18421 aaaagatagc atatttttct gtaaaaaaca ataaaattat cgttctttct ttacacgttc
    18481 agcaaattca tagaaaggag ccaatttcgt taactgttga ttacattgat cactatcatg
    18541 acaaatataa ttcccctttt tcgtatacgt gccatcagaa ccggctttcg ttgtagaaag
    18601 aaagagcgag acattcgatg ttttctgaca gatcgcacag attcccttat taatcgttgg
    18661 tgataaagta ccgctgatcc cttttaattt tccatcttcg taataaacca tgaatttttt
    18721 ttgcgtccct ggatcattcc aaccgatgta cgtatgtgct tgcaagtcga tttcattcca
    18781 agcgggttgt ttgagttttt tcacttttcg gaataatttc tcgatttgtg gtttgctcgg
    18841 ctgcataaac ggctggacga tttgcttcag ctcctccaat gctcgttctg cttgcgtttt
    18901 tgtcagtttt acatctaaaa ctgtcgttaa aaacgtatcg atttctgatg gttcgtaacc
    18961 taatacatcg cgaatctttt cagctgctag agcctgaacc gtttgtaccg tttttgagtc
    19021 attgacagat gagtacgcat ttaataactg ctctacttct ctttttataa aaaaatattg
    19081 atatggtttc attgtttgtt ccatgatttt aaagtccttt cttctaacca aagtgcatac
    19141 tttgtagaaa aaagtgatct tctgcaaagt atgtgttacc tgaagatccc ggactctttt
    19201 aaataagata cgttttgcat cttatcatca ccccctttaa tgttcattct tctatgtaca
    19261 tgattcattc gataaataaa agtaaaccgt atcttgagca gatagtcaac tgcaaacaga
    19321 aaaaggactg tgacaaaagt cgtgtcaaag tccctttcta caaacaaacg gtgttccaga
    19381 agtagcttct tcaaaaaata agccggaatt tcacaaaaat ttgagaagca attttcgtaa
    19441 attccttctt atttcttgaa gctgacccac ttctgtcaca acctcaatat ggtctattat
    19501 tggttttacc tgttaaatcg aggattcttt tccgaagtaa cgttgccatc tcttacgcat
    19561 acttgctgac ataggctgag cggcttcttt gactgcgcag tatttgtcta cgaatgttac
    19621 gatatatcct tctttgtact taggcggtgc aatcgttgcg ccccacatgt gcttcaaaat
    19681 gatatctcgc tcaagatccg acaaatcggt gatcttttct gcattcttta cagcgattct
    19741 tggatggata tacgcatgtg tgccttcatc aaatttcgtc gtacgccaat catagtagaa
    19801 cagatcatgc aaaagtccag cacgcgctgt tgctttagca ttgccacccc atttttttgc
    19861 gagcagataa ctatagtaag aaacactgat ggaatgctcc aaacgagtgg aatgaacatg
    19921 ctgtgtgtaa ttcgctaatt ttttcactgc ttctgtttct aaaagatctt ctacataaga
    19981 catatattct tcgtcttcac gccatttttc tgtcataatt atcgatctgc cacctttttg
    20041 aatttcattt tcttctgaaa ttgatctaag tataccacca gtgttcggat tattctagtc
    20101 gccttggata aaatagccaa agcttaatag ttccttaatc ataaagaaaa atgataaaga
    20161 acaatccctg ccgcaacccc aacattcaac gattccgcat ttcctttcat cgggatatat
    20221 acgttctgat cagtcaaaga caagatttcc tgacttaccc cctgaccttc gttccccatg
    20281 attattgcga agttatctga tttttctact ttgtctaatg cgtgtgcttc ttcatttaat
    20341 tctgtgccat acactgtaaa ccctttgtct ttcattaaag gaatcacttc tgtcaaatct
    20401 ttttgcaaca caggcaaatg ataattgctt ccctgcatgc tgcgcagcac ctttgtacta
    20461 taaatatctg ccgttccttt gcctaataaa acaccagcca tatcaaatgc atctgccgta
    20521 cggatcatcg tccctacatt tccaggatct tgtacattat ctaataacag ccatcctcct
    20581 gaaaaatctg gcgtgccttc agccgttaac gccatcactg ctaagattcc ttgtggtgta
    20641 ggtagatccg atacagcatc catgacctca tccgaaacta gaaaagtaga aatctctttg
    20701 tttgcggcca gccagtctcc ccattctttc atgcctcgct gtgtcacaaa aagttctttt
    20761 atttctgcat cagacgacgc agcttcttcg atcagatgga acccttccaa aagatattct
    20821 tccttttctt cccgatattt ctttttatgt agttttttta attctttgat tcttggattt
    20881 tttgctgatt ggatctcttt cacattcctc acctccccat gattatatca tggaataggt
    20941 gcttgagctt gtttcatcct aaaaaaatcg gtatcattaa tttataaaat aggaaagaaa
    21001 ggtgacgaac atgcgcaaag ttaaaatgaa cgtccaagga cgcgtccaag gcgtaggatt
    21061 tcgctacatg accaagatgg tcgctgatca actaggcgtg actggaacgg taaaaaacga
    21121 agacgacggc tcagtcacaa tcgaagcaat cggcaatgac gacatcatcc agaaatttat
    21181 tgaagaagtc aagaaatcac cgagcccaag tggacgagtg cagtatgtgg atgtccaaga
    21241 agacccgatg attgaggaac gaaagaaatt tgatgtggtg ggataaggtt gtgaaaaagc
    21301 tggtctgcat caagcattaa ggacaggttg tgaaaaatag ctctccatat ttttcaacag
    21361 gctggactta atgccgtaga tgcaagcttt tgaacaccgt caataaggtt gtgagaaagt
    21421 cgcttagttt tgagtagtaa ggagaatttt gtgaaaatgg ctttctacat tttttcataa
    21481 aatttgactt aataccgaga aactgacttt tgaacaccgt cgataaggtt gtttttgagg
    21541 cgctttgcct cgagtattaa ggagaaacat tttcaaaata gcttctcata ttttgttaat
    21601 accgagaaac tgacttttga acaccgtcga taaggttatt cttgaggcgc tttgcctcaa
    21661 aaattaagga cagcagtgaa aaagctgatc tatcacttgc agtgagttca tttgactccg
    21721 ctttttcggg cagaaaaaaa gtttttagtt tattaaagta agaaagaaac acttggaatc
    21781 attctacttt ttcctagtat ttctttcttg attttttgtc tttttttcaa aatttttcgc
    21841 tacgtaaaag aacgaatatt tatatgaaat ttctatgaag ttgattgaat tccatgcgct
    21901 tttttagtat agttaagttt gtgtaaaaat tatagaagag gaagtattct atgaataaat
    21961 ggaaaaactg gttattggga tccggacttg tcagtatcct gctcttttta tctggctgtg
    22021 tgcggatgga ttcaaacgga aaccctgaca cttcgggaat cgtttatcga gtactcgtcc
    22081 atcctatggg ccaagcgatt acatatctag ttgaaaattt caattggtct tatggctggg
    22141 cagttattgc aatgactgtg atcgtccgta tcatcatctt gccactaggt attagtcaat
    22201 cgaaaaaaac aatgatccaa tctgaaaaaa tgcaagcgtt aaaaccacaa gttgaagcag
    22261 cccaacaaaa attgaaagct gcaacaacac gtgaagaaca aatggcagca caagcagaaa
    22321 tgcagcaagt ataccgcgaa aacggattga gtatgaccgg cggaatcggc tgtctgcctc
    22381 tattgatcca aatgccgatc ttctctgcct tgtactttac ggcacgttat acagaaggta
    22441 ttcgggaatc atctttctac ggcatcgatt taggctcacc aagtatggta cttgttgtca
    22501 ttgcaggggt cgcttatctg cttcaaggat acatctcaac gatcggtatt cctgaagaac
    22561 agaaaaagac gatgcgtacg atgctgatcg tctctcctgc tatgatcgtc ttcatgtcga
    22621 tcagtgcgcc agccggtgtt acactttact gggtagtcgg tggtgtgttc agctgtctgc
    22681 aaacattcat cacaaacgtt atgatgaaac cacgtttgaa agcacaagtg gcagaagagt
    22741 tgaaacaaaa cccaccaaaa caagtcgtca ctccaagaaa agatgtgaca gaatcagcta
    22801 aaccagctga cagcaaacaa gcaaccaaac aaatcaaaca accaaaaaac agtacaaaca
    22861 acaaaggccg caacgcagga aagcagcaga agcggaaata aaaatatatg acaaaaaact
    22921 gtagacaaat aaaaaaattt ctttgttcta cagttttttt gttacaatca aagaaaacgt
    22981 tcgcttttcg aggagggatg tataatgatg ttcaaacaat caatggatag tcgaatggaa
    23041 gaagacaaaa tttcgctaga tgcacttgtt cctaaaaatc atttagtccg taaaattgac
    23101 aagttattgt catttgattt tatttatctc attgttgaat caacttattc gaccatcggt
    23161 cgacctagcg ttgatcctgt cgtcttagta aaattagtat tcattcaata tttatttggg
    23221 attcgttcga tgcgtcagac cattaaataa gtttaaacaa acctagctta tcgttggttt
    23281 cttgggctta gtttaattga taaagttcct catttttcaa cttttggtaa aaactatgca
    23341 cggcgtttca gagaaaccga tttgtttgaa cagatattta tcattattct ggaacaagca
    23401 gttgttgcta gatttattca tgaagaaatt ttgtatatgg attcaactca tattaaagag
    23461 aatgcgaaaa aacgtatttt tacaaaagat atggcacata aagaagccat tttataccaa
    23521 aaacgattgg aagaagaaat caatgaggca agaattgccg aaggcaaacg ccattttggt
    23581 tcaatcattc gtcaggaggt ggtcgaaagg aaatttagcg tcgcagatcc agaaattggt
    23641 tattatgtaa aaacagaacg agaaaaacag tttgcttatt cttctcatac tgtttgtgat
    23701 gaaaatggtt ttgtattaga tgtcatgatt acgcctagaa atctacatta cagccgaatg
    23761 ctcgtgccac aaatcgaacg tgttaaatca cgttatggtg tagctgctga tgctgcgtac
    23821 aaaacacctt ggaacgccaa gtatttaatc gaccgaaaac tacgacccac ttttccttat
    23881 actcgaccca aaggaaacaa agaacgtttt aaaaagaaag attttgatta tgatccctat
    23941 tataactggt acctatgccc gaatgatcaa acacttcagt ttcgcacaac tactcgagat
    24001 ggctataaaa aatatgtatc taaatctttt atttgtgaaa gttgtccatt attgaaaaat
    24061 tatacagaaa gccagaaaca ccaaaaagaa atacatcgac atatttgtga agattactta
    24121 gatgaagcag aacatttacg ccatacggtg tacaatcgcg aaaaatataa aaaaagaaac
    24181 gattgaacgc atttttgcag acgcaaagga aaagcatgga gtgcgctaca ccaagtatcg
    24241 aggcttggaa aaaatcaggt gcatacgatg cttgtttttg tcgcaatgaa tctgaaaaaa
    24301 ttagccactt ggcagtggga gcatatgtct ttatatcttt tattatacaa aaatagacaa
    24361 aagtatcagt ctacactctt agtcaagagg aaactgatgc ttttatctac agtctgagag
    24421 agtgggacaa tagtcctttc tccaaaagaa aacaagtccg actttgatag ttaattttaa
    24481 aatcggactt gttttttctt ctttacgtct tttcctctcc atgtatttcc ctaattttct
    24541 taaatttaac gccatgagtg ccaagccaat gttaatcttg gctccttgtt tccctcgtaa
    24601 gtggaaatcc gtttccgata gatcgaacct tctttttcct ctaaaaactt tttttacatt
    24661 ctgctttgaa ataacgccat gtattattca ctaaaatttg tcggtttcgc tcactttttg
    24721 ctttcgtaca ttgactacga acagggcagc accaccataa gccgcctgcc agttacctct
    24781 cactcttacc gggtctttca gaacacccgg atgctctaac acatcactcg gctaggctcc
    24841 gttattgaag aagaaagccc catatctttc catggcatcc tcggccagct taagaagctc
    24901 ctccatctgt tcggagacct tcctcacata tatgggcaaa ggccatgaag acctccacca
    24961 gactgttcat gaccttcttc agtgctgcga tcatctgctc tctcttctcc atggcttaca
    25021 cctactcgac agaatcagcg gcatcagagg caagagcatc cttcatgtgc tgtgccagga
    25081 agtcatccag ctggttcata gaaaaatcga cacccttgtt catggcctcg ctgatttcca
    25141 tcagcttctg gttcacctct tcctcagaca ggccggtgcg ctcacagatg gaatggagca
    25201 gatgatcgtg catctctgcc atcttctggt ccatgtcgaa tttttcgtgg cagcaatctc
    25261 tgcgccatgc cttcggaaat acggcttccc cccctcatca gactgtgtgt ttggttccag
    25321 ctctttcttc cgggattctt cgctctcgga aatcggtctg tacagtgaac acacataaat
    25381 attcttccta tgactggtat cactcatggc ttgtctcctt tatttgtttt gtcgagtcct
    25441 acctcaaagg aggtctggcc agcatatagg tgttcatctg actgtccctt tcaaaattag
    25501 tgtcctcatc tatgatttcg ggcgttttat aactcctccg gacaaaattt ttgaaaaaat
    25561 ttgtcgaggg cttaatacac ctctttcata actgcaaacg cacgcttcaa ggtcttggtg
    25621 atttctgcat gcgacatgcg cagatccctc gcaatctgct gctggttctt gttcaggaag
    25681 taataatgct cgatgacctt tctctgatta aaggttagct tctggatctc ttcttgcaga
    25741 cgcttacgct ccatcttctt aaaatcagtc ggagcaatct tctccacaat gtcaggagta
    25801 taggacatca tgcagtcctg tgtgtagctg tcctcgatgg tctgctctat gcctccgagg
    25861 tatcttgcat agttggattc cagacggttc tctgcatcat ggtcatgtag atggtccact
    25921 gatagtccat atactcctca accaggatga agctattctt atcggcgaac cgatacaaca
    25981 ttccattcat gcggtcagct gtgaagatgg agtacatatc attgcgaata tagaggataa
    26041 agtcgttgcg atacacggtc aacagagaat cgtccatcta gaactgagcc acactcactt
    26101 ggcaccatat tatcactcag ctaacggaag ccgggctgct ttacgccttt ggtgctgtcc
    26161 ttgatttcct tccatatact gcttgccgag attgatgatt cttctgacat cgatcaaagt
    26221 aacaggtaat ttagaatctt ccacaaacct gattatctcc tctactactt tcggctcaat
    26281 atcttctaat gcgatcacga ttttatatac ctgctacttc acaataacat cctgaacatc
    26341 agatattcgt ccaaaatact ttaatttcgc caatccatcc ccataattag caaatcctgg
    26401 ttcttcaagt agaatatcct ctttctttcg atctttcaca tatccaataa tatttgcatc
    26461 tgcgtctgga tctgataaca tggccaaacc atattcttta gccaggtttc cattttccac
    26521 aaccaaaact cgagtatggg acttgctgcc tatctttttc agtaaaaact gaaatacagc
    26581 attcttgacg agcaacaaaa cgcacgatat gatccaaaat aaaagcaatg gccatttgga
    26641 aaagtatttc acattagcga tatataagta taagaaagca aggaagaaca aacttccaat
    26701 accggttaac gatactattt taaaaatccg ttttgatact ttctgaaata ggtcttattc
    26761 gttgcacgaa aaatcctcaa aaaatctgca atcaaaatac tatatagcgc agccatcatc
    26821 aaatacggaa ctgacaacat attgatcccg ggagcagctt ctaaaacgtg gtaacaaata
    26881 aacatggcca atagataggc tacaaaaata gatgctgcat ctccaataca gtcaaaaact
    26941 gtgttccaaa atcgttcctt gataatttca ttgattctcc ccttataata atatcgaatg
    27001 ctccaacatc ggtcagaatc atctcttctg tctatcattc gccacgtaga agttgttgga
    27061 aaagagtgac aaccgttcaa gacgaacggc tatattgtat taattattct gtgtatcaac
    27121 taaccactca acaaattcac gcactgcacc attaccacca tcatgttcag cgataaaatc
    27181 tgaaatttct agaacctttt taactgcatc gtgcggacag gccacaatac cacctgcact
    27241 tttaacagga tgcatacaag ataaatcgtt tatatcgtct ccaatatatg caacagtact
    27301 tagatcacta gtaatctcaa gcagtttctc tattttattg tctattccct gataaagaat
    27361 gttgatacca agttccttac atctattttc taatattttg gaacgacgac cagtgataat
    27421 aataggctca atattatggg ctcttacaat atcgtgaata gcgcaaccat ctttaatatt
    27481 gaacgccttc ataatttccc cacactcacc catatatatc tttccgtctg ttagagtacc
    27541 atcaacatcc ataaccagaa atttaatttg gctcatacac tttccccccc tttcaataat
    27601 tgatagaaat cttaaataca gctttcttta ctttagctga ttcattcact cttatgcaag
    27661 gcatatgagc atcctcagga gttagtagca gcagcttccc tccttctaag agtacacaat
    27721 tgccaactat tgaattactg taaaacacaa tatccttgtc agctgaataa tctacttcaa
    27781 tctgtaaatt agatatagat tctactacaa tatactcgca tcctaagaga atacattgaa
    27841 tgtctaaata tttcaagtga gattcgtaca ttctttcttc aaatctatat atatcatagg
    27901 actcaacatt cagataaatt ccattggaaa gatcatatcg accttcttct aattccaaca
    27961 aggaattttt tataagaaaa tatagacttc tggaaattca atttccattt ttttagactt
    28021 aactttttca tttatctcct ttatgcaaca ataccagctt ttacaatttg ctgccatgta
    28081 atggtaccaa caagcatacc ttcatcatcg attataggaa gaatgttaat actattagcc
    28141 tttaaaaatc tcaatgcatc gacagctaat ttatctttag taaatacttt gggattagca
    28201 atcatcacat catcaatttc tacagaatag atatctactt ttctctcaat aattcgtctt
    28261 aaatctccat cagtaataat gcctttgaga gttccatcct gatcaatgac tgaaactata
    28321 cccaaccctt tattcgacat aacagaaata gcatccatta acaatgttcc cgaattgacc
    28381 attggaatct cattgccgtg ggacattaga tcagacactt taagtattaa ttttttgccc
    28441 aatgaacctg cgggatgaaa ctttccaaaa tcagaatctt taaatccata aataccgcta
    28501 gcaacaacag ctaatgcatc gccgtaacac aacacacttg tagcactaga agttggagcc
    28561 aatcccaaat agcaagcttc ctcaaactcc ggtaatatct ggacaatatc agatgcggta
    28621 gcaagcgtag attttgaatt gcctgttatt ccaattaaag tagccccaat cattttaata
    28681 ttaggaagaa ttttaacaat ttcatcggat tcgccgctat agctgatagc aattacaata
    28741 tccttttctg aaaccatacc taaatctcca tgcatcgctt cagcaggatg aagtctgaat
    28801 gctggtgttc ctaagcttgc aaatgtcgct gccagcttag cagcgatatg acctggcttt
    28861 cccataccag tgataatgac ttttccttcg caattaacaa ttgcatcaag aatacgaacg
    28921 aaagtcttcc caagacaatc tctcgttctt tttaaggcct caatttcaat atcaaaaact
    28981 cttttggcct ctattatctt atccatttta tttacctctt cagtttttaa tagcagctgg
    29041 aggtctttca catgttgtaa cacagtacaa aatgtgaaag acttaccata catttctatg
    29101 caatgcaaga aaaattatct aacggcattg tatactttta gtacacgctc taataacccc
    29161 tcaaattcat ccaagagaac catattcgga ccatcagaca aggcattgtc aggatcagga
    29221 tgtacttcaa agaacaaagc atctgcgcct gttgcaacag ccgccttcgc aagaggctca
    29281 acgttatttc tattacctcc tgtaacatct ccttttccac caggcttctg aacactatgc
    29341 gtagcatcca taacaacagg gtaaccaaac ttcttcatct caacaataga tgtcatgtca
    29401 acaaccaatg tattatatcc aaaagaagtg ccacgttcgc aaagcataat gttactatta
    29461 cctgcctcca caagtttatt aataacattt ttcatatccc acggtgcgag aaactgtgct
    29521 ttttttacgt taattatttt gcctgttttt gctgctgcca ctagtaaatc agtctgtcta
    29581 cacaggaatg caggaatctg aagtatatcg catacctctg cagccttttc tgcttgccaa
    29641 ggttcatgga tatctgtaca aattttcaat ccataagttt ccttaacttt cttaaggatt
    29701 ttcaaaccct cttcaatacc aggacctctg taagaggtaa tactagttct gttagctttg
    29761 tcgaaagaag ccttaaaata ataatcaata tcgagcttct ccgtaatctc cttaatagtc
    29821 ttagctatta acattacatt ttcttcggac tcaataacgc atggtcctgc aattaacata
    29881 agtctattca tcttatagac cttcctttct taattcatct tctacctgct gtatctgctc
    29941 cggcgtatcc acccccacgg tttgatattt agtttccttt actcgaatct taaatccact
    30001 ctccaaaact cgaagttgtt cgagagattc agatacttca agaggcgttt tgggcattgt
    30061 actgtatttc ttcaaaaacc atgttttata cccataaaca ccaatatgtt taaaatgcag
    30121 agaatttttt ccatctcttt cgtatggaac acaaaaacgt gaaaaatata tcgcataatc
    30181 attgacgtca ttgattactt taacaacatt tggattgtca agttcctcct gaacttcaat
    30241 atgttttttc aaggttccca tataaacttc atcgttatca aagcaagaat acaaatctgc
    30301 tatcatctct gccttgataa ggggctcgtc tccttggata ttaagaacta agtcttcatc
    30361 ccggagttga aggatctctg cgcactcagc aagacgatct gtgccgcaag tatgtttatc
    30421 agaagtcatc aaagccattc caccaaagct ttcaacacag tcaaaaattc tcttatcgtc
    30481 tgttgcaaca tatactgcat ctagcatagc tgccttagaa acattttcat aaacccactg
    30541 aatcatgggt ttactagaaa tcatagcaag cggcttccct ggaaacctac ttgatccata
    30601 tcgagatgga ataatagcta ttgtcttcat cggttctttc tccttttaag ataatccatt
    30661 aacaacttat ctttcataca tattagtaaa atactatata gtaaaataca caaaattact
    30721 gtaatgaacg ttgatattac tgaatttggg agcttactac tgacatatcc tccgacgcct
    30781 cccataatca gtgcacctat caaatagttt tttacattcg taataattga tgccattttt
    30841 aattctatac agtttcttac ttcataaaac tgaattgcag taactgtaag ttcagcaaat
    30901 attgtaccta ttgcagcgcc tatagcttcc aaatgtggta ttaaagccaa attgacaacg
    30961 agattaatca cagcgcccgt cataatagag aaggtataca ccctttgtct tttcatggga
    31021 atcaaaacag cttgaccaac cacactagca aatccaatta tcaaaatcaa tggtgatagt
    31081 aactgaaata aggaaactga cttagcgtat ccgtctccaa gaaaccacgg tataaaagta
    31141 tttgcgatta taatcgcacc aactaccatt ggggcgccta tgcaaaacac aaatgatact
    31201 gcattcaata cttcttttct aattctatca atattgtttt ctttaaccgc tactgcgata
    31261 tgtggcaaca taactgcacc aagagatgta atgactgtca tcaacagctt cactattttt
    31321 tcaggctgcg agtatagccc aacttggaca tccgtagaaa atattcctaa ta
//
LOCUS       DAIGOK010000019        31351 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.019, whole genome shotgun sequence.
ACCESSION   DAIGOK010000019 DAIGOK010000000
VERSION     DAIGOK010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.019"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            211..1080
                     /locus_tag="NOA01_000669"
     CDS             211..1080
                     /locus_tag="NOA01_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HCI0631891.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1289..2314
                     /locus_tag="NOA01_000670"
     CDS             1289..2314
                     /locus_tag="NOA01_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCI0631892.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2353..3303
                     /gene="manA"
                     /locus_tag="NOA01_000671"
     CDS             2353..3303
                     /gene="manA"
                     /locus_tag="NOA01_000671"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HCI0631893.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3397..5100
                     /locus_tag="NOA01_000672"
     CDS             3397..5100
                     /locus_tag="NOA01_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCI0631894.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5102..7135
                     /locus_tag="NOA01_000673"
     CDS             5102..7135
                     /locus_tag="NOA01_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCI0631895.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7146..7586
                     /locus_tag="NOA01_000674"
     CDS             7146..7586
                     /locus_tag="NOA01_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCI0631896.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7786..8913)
                     /locus_tag="NOA01_000675"
     CDS             complement(7786..8913)
                     /locus_tag="NOA01_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI0631897.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8916..9593)
                     /locus_tag="NOA01_000676"
     CDS             complement(8916..9593)
                     /locus_tag="NOA01_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0631898.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9857..11623
                     /locus_tag="NOA01_000677"
     CDS             9857..11623
                     /locus_tag="NOA01_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCI0631899.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11638..12075
                     /locus_tag="NOA01_000678"
     CDS             11638..12075
                     /locus_tag="NOA01_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCI0631900.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12088..13239
                     /locus_tag="NOA01_000679"
     CDS             12088..13239
                     /locus_tag="NOA01_000679"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCI0631901.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13301..13999)
                     /locus_tag="NOA01_000680"
     CDS             complement(13301..13999)
                     /locus_tag="NOA01_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCI0631902.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(13992..15344)
                     /locus_tag="NOA01_000681"
     CDS             complement(13992..15344)
                     /locus_tag="NOA01_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HCI0631903.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15567..16967)
                     /locus_tag="NOA01_000682"
     CDS             complement(15567..16967)
                     /locus_tag="NOA01_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCI0631904.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17158..17865
                     /locus_tag="NOA01_000683"
     CDS             17158..17865
                     /locus_tag="NOA01_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCI0631905.1"
                     /translation="MKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCCAPCSTHTLEF
                     MSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIVDEYRPNEFTK
                     MVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISPKKNAALINQI
                     GMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAKQQGCDLKEIN
                     REAKAYLKTSTVVFEEN"
     gene            17990..18469
                     /locus_tag="NOA01_000684"
     CDS             17990..18469
                     /locus_tag="NOA01_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HCI0631906.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18466..19233
                     /locus_tag="NOA01_000685"
     CDS             18466..19233
                     /locus_tag="NOA01_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631907.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19463..20032
                     /gene="lepB"
                     /locus_tag="NOA01_000686"
     CDS             19463..20032
                     /gene="lepB"
                     /locus_tag="NOA01_000686"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCI0631908.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(20136..20738)
                     /locus_tag="NOA01_000687"
     CDS             complement(20136..20738)
                     /locus_tag="NOA01_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCI0631909.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20844..21313
                     /locus_tag="NOA01_000688"
                     /pseudo
     CDS             20844..21313
                     /locus_tag="NOA01_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            21419..22003
                     /locus_tag="NOA01_000689"
     CDS             21419..22003
                     /locus_tag="NOA01_000689"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCI0631910.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            22006..23079
                     /gene="prfA"
                     /locus_tag="NOA01_000690"
     CDS             22006..23079
                     /gene="prfA"
                     /locus_tag="NOA01_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCI0631911.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            23072..23911
                     /gene="prmC"
                     /locus_tag="NOA01_000691"
     CDS             23072..23911
                     /gene="prmC"
                     /locus_tag="NOA01_000691"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCI0631912.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23904..24083
                     /locus_tag="NOA01_000692"
     CDS             23904..24083
                     /locus_tag="NOA01_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631913.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            24105..25130
                     /locus_tag="NOA01_000693"
     CDS             24105..25130
                     /locus_tag="NOA01_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCI0631914.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            25305..26549
                     /locus_tag="NOA01_000694"
     CDS             25305..26549
                     /locus_tag="NOA01_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCI0631915.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26702..27649
                     /locus_tag="NOA01_000695"
     CDS             26702..27649
                     /locus_tag="NOA01_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCI0631916.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27783..28412
                     /gene="upp"
                     /locus_tag="NOA01_000696"
     CDS             27783..28412
                     /gene="upp"
                     /locus_tag="NOA01_000696"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCI0631917.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(28551..28793)
                     /locus_tag="NOA01_000697"
     CDS             complement(28551..28793)
                     /locus_tag="NOA01_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631918.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28914..28986
                     /locus_tag="NOA01_000698"
     tRNA            28914..28986
                     /locus_tag="NOA01_000698"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28947..28949,aa:Thr,seq:cgt)
     gene            complement(29105..29743)
                     /locus_tag="NOA01_000699"
     CDS             complement(29105..29743)
                     /locus_tag="NOA01_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCI0631919.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29866..30498)
                     /locus_tag="NOA01_000700"
     CDS             complement(29866..30498)
                     /locus_tag="NOA01_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCI0631920.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            30849..31148
                     /locus_tag="NOA01_000701"
     CDS             30849..31148
                     /locus_tag="NOA01_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCI0631921.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT        10695 a         5467 c         6305 g         8884 t
ORIGIN      
        1 gaggagctat ttttgtttgg ctgttcttat tacttgatgc agaacagctt tttcacaacc
       61 tctttttata agagttttta ctcattaatt tacaaaaaac aagcaaaatg cgaattttct
      121 cacatttttt ttaacaaaat caaagttatt gcttgaaaga agaagaatcc atcgttaaaa
      181 taaagcttga cgtagagggg agcaagaaat atgaaagatt ttcaattggg ggctgccacc
      241 tatttaaaaa gtgtagccat tcgggtgaaa gaccgagata aaatgattga tttttacaag
      301 aacgttgttg gctttgattt gaaaagagaa gaaaacgaat tagccatttt aggaacagaa
      361 gaagctggaa gtgaaatgtt gctgctggaa gaaagtccta gagcgaatga tcacatgggg
      421 gtgatcaaaa agctgaaccg tttttcattg attatcccta gcacagaaga aatggcagat
      481 atcctttgtc gtatccgtaa aatgaattat ccaatcaaag aagctttaga agatcatgga
      541 cgtatgggga ttttgctcgt agacccagaa gagaatgaat tggaaattta ccatgaagag
      601 aataagaacg accaaagaga aattccagaa acaatggatc aagaagcatt attaagaaaa
      661 tctgaagaaa aattcagtaa actatcaaca ggttcttatt ttgaaaaaat ccatttaaat
      721 gttactaact taacaaaaga acgccaattt cttaatcatg tacttggttt gaaggtacat
      781 aatgaagaag agggcatcca tgtattgaat gaaggagatt tccatgttgg tttgacagaa
      841 gctagcggag gcgcaattga tttgcctaca gatgaagtga ttggacttga ttttctgaaa
      901 ttcatgatcg atactgaaag catggaagga ctgattgctc acttgcagca actatcagtt
      961 gatttcttct tagacaaaaa gcacacagta ctcacaatct atgatgctat aggaatcgaa
     1021 tggtggttcg tcagtgagcc aaaagaaaag aataaaactg acattcgatg cgacgcttaa
     1081 ctttttcttt cttaacgatt gtcgcatgtc tcgtcatttt atgaacggtc atactatcgc
     1141 ttattctaga gatagaaaga gaaaatagta taccggggga aactgcgcgg tttttcgcgc
     1201 agtttttttg tgtaaaaaag gttgattcct catagattaa gctttaagct tggactgtaa
     1261 gaatctgata gaaaagcagg agaaaaagat gtatccacac acgaaacagc ttattttgaa
     1321 aaagatggaa gaaggatttt atccaggtgt agcagtcgcc ttcataaaag aaaatcaagt
     1381 agaaacattt acagccggct ttgcccaagt cgttcctgaa aagcagctga tgacagaaga
     1441 tctgttgttt gatgcagcct cactgacgaa agtcgttgca accacttcat tagttttacg
     1501 tctattagaa gatggaaaga tagaactcga tcaaccgctt cataaatatc ttcctgaatt
     1561 tgaaaatcag acaatcactt tgcgtcattt actgacacat acttctgata tcaaaacatg
     1621 gataccaaac agagataagc tatctcagga agaattgatc gccgcttatt tgaagctaga
     1681 accaggtgag caactaggaa aagaagtaaa atatacagat gccggtacga ttcttcttgg
     1741 gttgatgcta gaggaaattt accaagaaac tgcagtggaa atctttcaag aacaagtatt
     1801 ggaaccatta ggaatgatga acagcttatt tcttcctttt ccttccaaaa aaatcgttcc
     1861 cacagaacaa ctgccttcgg gtgaggtttt gcgtggacaa actcatgatc cgaaagcaag
     1921 agttttggct gaacatgcag gtaacgcggg tctctttatt gatttgaaag acagcattac
     1981 ttttgtccaa atgtatttga acaagggaaa aatccgtaat ggtcgttttt tagaagaaga
     2041 gacgattgat tctttactgc aagatcaaac accgaaaaaa aatggctatc gttctttagg
     2101 atgggactta aaaaaggatg tagaagacgg tcatgcgatt ctttttcata ctggttatac
     2161 aggtacgttt ttcttgattg atataaaaac acaagaagcg ttgatatttt tgtcaaatcg
     2221 cgtacatcct gtcgatcatc gtgcggagta tctcatatgt cgagatgaaa ttgttgaaaa
     2281 ctttttgaaa gagaaagcgg tattaccgaa ataatgctat aatagctatg aaaaatgaga
     2341 ggagtttgaa ttatgcagga acctatgttt ttaaaaccag tttttcaaga aaaaatttgg
     2401 ggaggaagtc gtttacgttc agtctttggt tttgatatcc ctaacgataa aatcggagaa
     2461 gattgggcga tcagtgcgca tcctcatggt gtcagtgtag tagaaaatgg cgaattcaaa
     2521 gggaaaagat tagatgaact atggtcagaa cacaaggaat tgtttggcca tccttcagaa
     2581 ccagtgtttc cattattgat caagatctta gacgcagaag atgatctatc tgtacaagta
     2641 catccagatg atgcctatgg gatgaaacat gaaggtgaat taggaaaaac tgaatgctgg
     2701 tatatcatcg atgctgaacc tggtgcagaa atcatctatg gtcatcatgc aaagacaaga
     2761 gaagaactag ctgagatgat caaagatggg cgctgggatg accttttgaa gaaagtacca
     2821 gttaaaaaag gtgatttctt ttatgtacca agcggaacga tccatgctat tggtaaagga
     2881 atcatgattt tagaaacaca gcaaagttct gacacgactt accgcgtgta tgactacgac
     2941 agaaaagatg cgcaaggaca aacaagagag ctgcatatcc aacaatcgat cgatgtaaca
     3001 actgtccctg cgaaaacacc agaattacaa atcaaagaag tccgaaaagg gaattcatcg
     3061 atcgtcactt atttagaaac tgagtttttc aatgtatatg agtgggatat taaaggaatc
     3121 acatctttca aaaaacaagc cccttataca ttgatgacgg taatcgaagg agcaggagac
     3181 cttgtcgttg acggaaaaac atatccatta gaaaaaggaa caagctgtat tattccaaat
     3241 ggcgtttctg agtgggcggt ccaaggtgaa ctagcaatca ttgcgtctga accaggcaaa
     3301 tagttcccag atagctgaaa gcagcagacg gtttttcgtt tgctgctttt tctacataaa
     3361 atccgtccat caataaatag aagtctggtg aaaaagatgg aatacaaatg gaaagcaaac
     3421 aacagcagtc gcgtgtctat gaaacaactg agaaaagctg gacatcactt aaatgaaatg
     3481 atcatgccaa atctaagtct gtttatcgct tggggaatct tgtcattagc aacaaaaggt
     3541 gtgtctggcg atttgcatac cacattagaa gaggtatgcc attggatgat ccagctgctt
     3601 ttacccatct tgatcagtta ttcaggcggg aaatcggtag ggggacaaaa aggggcagtc
     3661 gccggtgcaa ttgcttcttt agggttgatc gctcgttcca atgctccaca aatcgtagga
     3721 gcgatgatta tcgggccgct tgccggcatc gtatatgaac aatttgtttc tagattccat
     3781 gtgaaattca aggctggata tgaaatgctt gtcagcaacc tactagtagg actgactggt
     3841 agcgttattt gtataattgg aatcgttttt attgaaccag ttttaaatag tgttcacttg
     3901 ttgctcgcct ccgttgtgtc ttggctagtc acaatgaatc tgttaccact tgtcagtctg
     3961 atcttagaac ctctaaaagt actatttttc aataatgcaa tcaatcatgg tgtattgaca
     4021 cctttaggaa tcgaattctc acaaacagtc gggtactctt ccttattttt gatggaggca
     4081 aatcctggac ctggtttagg gatcttactt tctatccttt gttttgcaga aaaacaggaa
     4141 aaagtaaatg cgagtggagc attgatgatc catgcgattg gcgggattca cgaaatctat
     4201 tttccatttg tcttgctgcg accgtatcta tttttagctg taatggctgg tggggcaagc
     4261 ggcacgttga tatttcaatg gttagatacg ggattagctg cacctgtatc gcctgggtca
     4321 ctactcgtga tttttgccaa tgcgccttat ccacaactag ctggggtaag tatagggata
     4381 gcaatcagta catttgtgac cttcatttgt gcagcgatac tattgaaatt accaaataaa
     4441 gaaaaagaga acaaaacggt caaagaaagg ggagaaatct tggaaccatc tatcattcat
     4501 tccgtcattt ttgcttgcga ctcaggtatg ggatcaagtg ctatgggagc cgccctgctg
     4561 aaaaagaaat tagcagcaga aaaaatcgat ctttctgtcg gatacagctc tatctatgaa
     4621 ttgaataacc aagtaaatca gctggttatc gtgcaacatg aactagcggg tcttgcaaga
     4681 gaaaaagtac ctcaaagtca aatctatgta ctggatcact ttttggaaac agatatcttg
     4741 ctgtcacgaa taaaagaagc gatccaattt ccaaagcggg aagaaaaaaa agagatagca
     4801 gatatcacag aacaaaaaac gtcgcctgct aatattgtat ttttgtataa aaacaacaga
     4861 cgcggctcac aaactatggg catgacgatt ttaagtcaat tagcaaaaaa agcgaaaaaa
     4921 cagtttgtta tctcaaaaga atcaatagaa aaaatcaaaa tccaagaaga aacgatttat
     4981 atcgcacaaa gagaactagc tgtaactgaa cagctagaag aaaagatccc tcggctagtt
     5041 ctagttgaac attgggtagc tacgaatgaa tacgaaaaac tcgtgaaggg agaaatataa
     5101 gatgtattta tcgcccagag aaaaacaatt attagcagaa tttctcagta gcccgtcacc
     5161 tgtatctatc caaaaaatga tgaatttatt gaaggtcagt aaacggacta tttaccgcga
     5221 attagataat cttgaagtct cattgaaatc ggtaaatgct tctttgaaaa aagcagctag
     5281 aggctcattt gtcattgagg catcagaaga agtattagag caattaaaag aaaatacaga
     5341 tgggaaaagt aatgacttat ccactgctaa acgacaacac gcgattttag ccgagttgtt
     5401 actgacgaca gagccgttga gtcttgctca tttcatggaa tcatatcgca tcagtaatac
     5461 cactttttat gcagatatca aacagttgga agaaagtatt cgccaactgc ctttggaaat
     5521 cattcggaac cagggatacg agatcgaagg accggaaaaa taccgccggc ttctgacagc
     5581 gaatgtatta gaattagaaa tcaatgaata tgaacttttt catagtattt ctttcgatgc
     5641 atcactgaat tatttttttc gattcgttga tccgcaacat ctatctcttg cacgcaaagt
     5701 aatcggggaa gaattgatcc aaaaaaagac caatctaagt gatagaaagc ttgaacattt
     5761 ggttttgatg ctgacattga cgatggagcg tgtgacgaaa aatcatttac tgacaaatga
     5821 gacatataat agtctggcaa acaaagaatt actacagaca gctaaacgat tgttttcacg
     5881 gatcgcttct gagacaaaac aactttatcc agtaaatgag atcgtctttt ttgcaggatt
     5941 attaaatgac ttcacgaact catttgatca gaatttcttt gaagagacgt tcgatacaca
     6001 gcttgcttat ttagtaaaac aactgattca agaagtaagt gaagagacga atgtccattt
     6061 ttatgaagat gaaactttgt acaaaatgct gttgactcat ttatctggtg tgttttcaag
     6121 agccgtttta caagaagaga cactgaccaa tccgatttta gaaaaaatca tggttcaata
     6181 cgaggaagtt gcgaatgcaa tccgtaaagc agtagagcgg atttttcaag aaaaaaaatt
     6241 atccgaagaa gagatcgctt atatggtcct tcattttgcg aattcattgg aacgaagtcc
     6301 gaaagaaagc aatatcagta ttgcaggatt ttcgccaagt ggattagctt ctactagcat
     6361 gttggaacta cgattgaaaa ggtattttcc cttcatccaa caaattcatt tctatcggat
     6421 tgctgattta aaaaaaatcg atttacaaga agaatatgat gttgttattt ctacatctat
     6481 tttaccagga tacggtggaa aatatatttt agtctccccg ctactgctag aagatgaagt
     6541 caagcagcta aaagaagctt tttcgcatat tgagaagaaa aaaaggcaaa aaattacgta
     6601 taacagcaat aacgagtgga tgatagaaga tacttatgag gaaacaatgc atttcatgga
     6661 tcaaatcaat caattactcg gtcgtttttt tatccaagaa ctggataatc ctgagacgat
     6721 ttccgaaacg attcgcagct tatttcctta ttttcctgaa ggactagtaa cggatgaaga
     6781 ggtggttcat cgccggctgc ttaagcgata tgaacaagca cctatcggta ttcctcatac
     6841 aaatatgggg ttattccata catccagctc attgatttct aaaccgatct tttgtatttt
     6901 caatttgaaa catccgttga ccattgaagg gatggataga cagccagtcg atttgtcccg
     6961 tatgttagtt atgctggcgc cttcgccaat ccaagaaaaa gacgggattc ttttaggaaa
     7021 gatcagtgga gcaatcatca tgaatgactt gaacaccgag atcttccgct caggaaataa
     7081 agaaatcgtt tatcagttgt tagcgaaaat cctgattgaa gaaataaaga aatgaaggtg
     7141 aaaagatgat acagctacca gcagaaaaaa tattgctcga tcaatcctat caaaattgga
     7201 aagaagcgtt gaccgagatc ggccagtgga tgacccaaca agggggaatc aaaccggcat
     7261 atatccaatc aatgatcgaa agacaagaaa aagcgagtgt ctatatcggg aatttcgtag
     7321 ccttgccaca tgggactgac aaagatgaag aagtagtaga agaaggaatc tatttagtcc
     7381 aagtgcctga tggtgtggac tttggaacaa aagaggaacc aaaaatcgcg acactcttat
     7441 ttgtagtggc tttaaaaaaa gaacatcagt tagcggtgct ccaagattta gcctttttct
     7501 gttcagacat cgaccaagta atggcgctca gtgatgcgga aaccattgaa gaagtccaaa
     7561 tgatttttga acatggacat atataaccaa tgttcacagt aaaaaaagct gccttgcatc
     7621 aagtaataac ttcaaccaag ataaaaataa gaggagctat ttttgttggg aggttcttat
     7681 tactgcggat gcaagctttt gaaaactatt tattctagaa aaccgtttat ttgaaatagg
     7741 ggacggtgac aaggtttttg tcaccgcccc tattattttt gtgttttaat agctcttttt
     7801 tatatagata gtgcccatac ctatgaataa taaaacgata ctccatatac caagtgtcaa
     7861 gataccattc atgaaagtaa cctgttctga agctagtggc gagaaaatat ctcctcctat
     7921 gatcacattt tgaaaatcaa cataagaagt tggcaagtaa tgactgattg attctatcca
     7981 tccaatcgaa gaaaacaagc gatactctgc taagaaaaga caaagaagtg tacctaaaag
     8041 attgactaga gaatgttcaa ataaagcaga tagaagaaaa ctgattgtac ttaaaaagag
     8101 cacccacaac aacaggaaca gaaagctctg gaaaagatac tcatacatcg gcaggattcg
     8161 tatctcctga ccaatgatcg ttcctacagg ataacggaag tctcccaaac catttttgat
     8221 tattactagt atagagacga tcaaaaatgg catcacgaga cttagcatag ctgcaccata
     8281 ataaatggta aaacggatgc tatgtttctt taccatacta tccggtaata gattgactag
     8341 agagattacc tgctttcttt tttcataaga gatgatggaa gaaagaaaca aggcggttat
     8401 acataaaatc aaaaaactag aaatcattcc tgaaaaaata ttttcgtagt aatttgctaa
     8461 agagagatgc gctggcaagt caaaaataga atatctttgg atctgatgtt ctttcatata
     8521 acggagcaac tcgacacgtt tttgttgttc aatgattgga attccttgtc tttgaccaga
     8581 aatcagtcga tccagtatat ctttttcata atgataagtt gcttcgacca tttgctggac
     8641 attccctgtg ttttttgcat gtaagattgc ttcgatcagt ttgttgcttt tctctaaact
     8701 ttccacaaat tctttctcat tctcatcttc tttcaacgtt tcaatcaaac gttgcatctc
     8761 ttcatagtta tgattcagtt catcgaattc tgtataagca agtttatgat caaaaaaaag
     8821 tgggaatgca aagagcacga ccaacaatag aggaaataaa aagtttactt tgttttttag
     8881 taagagattg cgttcaaatg taagtaaaga catagttaca cctcatataa tttacgataa
     8941 atagctaaaa tctcttccat atttgaggat tctgtgacga tttctccatt ttttagaaaa
     9001 catgctttag aacaaatcga ttggatattc gcgatagcgt gggaagaaag aagaatcgtc
     9061 ttcccctctt tctgccattg tctgatgagg ctattcaaga tatcaatact tcctgggtct
     9121 aatccgttca acggttcatc gaataacatg acagatgaat cactgatcaa gtaagcagca
     9181 agcaagacat gttgtttcat tcctagagac atttttccaa tcggcaaagt aaggtactca
     9241 gtcatcttca atcgttggaa aacagtttgg cattctactt tgctctgcca atagtatttg
     9301 atcaaagtca aataatcaac agccgataga tagtcggata actgattcgt tgattctaga
     9361 aaaaagaaag accggcgata atctgtataa gcagtaggag agatgttgtg aagggacaat
     9421 tgatcataag ttgttggcag cagttgggca atgccattca gcaaagttgt tttgccaaat
     9481 ccgttcgggg caatcaaagc agctacttct cctgattcta ctaaaagatt caattgtcga
     9541 atcacacgtt ttttaccacg tttcatgttc atgtttttga tcagtaatgt catagatagg
     9601 cctccaatat aaaatttaat aacataatag aatggaaaac atctaaatta aatgctaaca
     9661 aaagttattg tttgcatatt ataaaaaaat taaaataaca gttttattta aaattgaagt
     9721 tttctgatac cgaaatcttt ttattttgct gacatactca aacctttggc acaaagtttc
     9781 gtgccaaagt acgactattt ctctttttag taagcggata caattagagt atcaaaagga
     9841 gagagggaga gacaaaatgg aacggacgat tcccaaaaac aacatttcgc taaaagcgag
     9901 ggtacaaaaa ttaggcagta ctttatcaag catggtcatg ccaaatattg gagcactgat
     9961 tgcctggggt gtattaacgg ctctatttat cccagatggt tatttaccaa atgaggcttt
    10021 cgctaccatg gtaagcccaa tgctaactta tctgattcct ttattgatcg gttatactgg
    10081 cggaaaagta attgctggtg accgcggatc agttgttggg gcaattgcaa cgatgggtgt
    10141 gattgtcgga acagatattc caatgatgct aggcgcgatg atcatgggac ctcttggtgg
    10201 atatgccatc aagaaatttg atcagctatt ccaaaaacgg atcaaatccg gttttgaaat
    10261 gctagtcaat aatttttcag caggtcttat cggttttgga ttagcgttat taggtttttc
    10321 tgcgattggt ccagttgtcg atgcattgac tcaagcaatg gcaaaaggtg tagagatcat
    10381 tttatctgcc catttgattc cattgacaag tatttttatt gaaccagcaa aaatcttatt
    10441 tttgaataat gcaatcaatc atgggatttt gacaccattg ggaactgaac aagtagcaga
    10501 ggctggaaaa tctattctat tcttgcttga agcaaatcct ggtccaggac ttggcgtatt
    10561 acttgctttt actttgtttg gcaaaggagc agcaaaatct tcagcaccag gagccatgat
    10621 cattcacttt ttaggcggga tccatgaaat ctattttcct tacgttatga tgaaaccgtt
    10681 attattctta tcagttatag gcggaggaat gtcaggaagt ttcattttcc aattgatgga
    10741 tgctggacta agagcgccag cttctccagg gtcgatcatt gcgattctag cgatgacacc
    10801 gttcaattct taccttcctg ttattttggg cgtgattgtt gccgcagctg tttcgtttgg
    10861 tatttctgca gtaattttga aagcggatgc gaaagataca aatgaagact tcgaaaaatc
    10921 tgttcaagag acaaaggcag ctaagcaagc ttccaaaggt gcccaaggta ttcctcaagg
    10981 agctacattc tctggcgtac aaaaaatcat ttttgcttgt gatgcaggaa tgggatcaag
    11041 tgcgatgggc gcttcgatct tgagaaaaaa agtacaggaa gcagacttgc ctcaaacggt
    11101 gaccaatcaa gcaatcagcc aactgacaga tgactcgaat acattgatcg ttactcaggt
    11161 cgaactacaa gaaagagcaa aacaaaaagc accgagcgcg cgttttgtct cagtagaaaa
    11221 ctttttgaat tcacctcgat atgatgaaat cattcaagaa ttaagcagca agcagaaaac
    11281 atcagtttct gaaattgaga atgagccagc agaagagttt ctttcagcaa acatcaaaca
    11341 aatcaaagag attctacttg tccacgatga tcgttccgga tcagcaacaa tggggatgac
    11401 cgtgctcaaa gatatattag acaagaacca tttaactatc ccgttaagaa aagtcaatat
    11461 ccaagattta aaagatgaac ctgattcact gatcgtagcc aaagaaagtt tagcggaaaa
    11521 agtacagatg gctgcacctc aatccattca tttatcagtc aacagcatgg tcaatccgca
    11581 aaaatacgaa tcgattgcac aatatttgaa acagacagct taagggagga aaacagcatg
    11641 caaagtttgc cattcgacat gatcgagctc aatcgatctt ttaaaaccaa agaagaagcg
    11701 atccgctatt gcggtcagat gcttgtagaa gcagggtgcg ttgatgagga atatatcgat
    11761 gccatggtag atagagacaa aatgttatct gtttatatgg ggaattttat cgcgattcct
    11821 catggaacag atgaagcaaa aaaatatgtg aaaaaatccg gcatctgtgt cgttcaagta
    11881 ccagatggtg tagattttgg taccgaagaa gaagaaaaga ttgcgactgt tttgttcggt
    11941 attgcaggag tcggagatga gcatttgcaa cttgtacagc aaatcgcact ttattgcagt
    12001 gatatggaca atgttgtcca gctagcagac gcgctcagca aagaagaagt aacggaaaat
    12061 ctagcaattg cttaaggaga gagaaaaatg agagcagtac attttggtgc aggaaatatc
    12121 ggtcgaggat ttattggaga ggttttggca gctaatggat ttgagattac tttcgtagat
    12181 gtgaatcaca cgatcattga tgctttaaat gaaagaaaag aatacgaaat cgaattagca
    12241 gatgaatcaa aagaaaaaat cactgtaaaa aatgtcaaag gaatcaacaa tcaaacaaat
    12301 ccagaaaaag taatccaaga aatcgctgaa gcagatttag ttactaccgc cattggcccg
    12361 aatatcttac cttttattgc tgagttgatt gcaaaaggta tccaagaaag agaaaaggaa
    12421 ggaagcacaa catctttaga tatcattgcc tgtgaaaata tgattggcgg ttcagcattt
    12481 ctagaaaaag aagtgtataa atatcttccg gaaacatcat ttactgacaa atatatcggc
    12541 tttccaaatg cagcagtcga tcgaatcgtc ccattacaac accatgaaga tcctttattc
    12601 gtccaagtgg aacctttcaa agaatgggtc attgacgata gccaaagaaa aaataaaaaa
    12661 atccaattaa aaggggtttt atatgtagat gatctagaac catatatcga acgcaaacta
    12721 tttagtgtca atactggtca tgcgactgtc gcttatacag gtgccttgtt aggttaccag
    12781 accattgatg aagcaatgca agatgcgtta gttgtggcac aattaaaatc agttttgcag
    12841 gaaacaggaa gtttgctgat tgccaaatgg gggtttgatg cagagcagca tcatgcgtat
    12901 attgaaaaaa tcattcatcg cttccaaaac aaaaatatct ctgatgcgat cacacgtgtc
    12961 gctcggacac cattacgtaa gttaggttat caagaaagat ttacacgtcc agtgagagaa
    13021 ctacaagaac ataacttgac ttgcccacat ttgacggcaa caatggggat tatttttaat
    13081 tactatgatc ctgaagatga acaaagccgt caattgcatg aaatgaagat ccatgaaaac
    13141 ttggaacaat tgattcaaga agtcacagga atcaatgatc cgaaaacaat cgggaatatc
    13201 aagcaaaacg tgaaccgtta tgccaagcaa gtcgcttaaa ataggatacg aaaaagggga
    13261 atgtgacgaa atttttgtca cattcccctt tttcgaataa ctaggctgtt tgtccttcaa
    13321 gatagggcag atccaattct ttaccgtaac gttgttctaa agctaggcgg atcaaacgag
    13381 ctgccaggtt ctcatttctt gcaaggatag gtccatggaa ataagaaccg aagacatttc
    13441 ggtaaatgac accttcagat tggtcttcac cattatttcc ttgaccttgt accactttcc
    13501 ccaaaggtcg ttcaccttca cctaagaaag tccgaccatt gtggttttca aatccataat
    13561 aagtttcatt gaattcttcg ttatggataa cgatatcacc gataaaccgg ttattttcct
    13621 gacttaacgt gtagtgatcc agtgcactga ttccttggat cttttctcca ttagctccca
    13681 tgtagtagtg acctagcaat tggtagccac cacaaatggc taacatgacg ccatcatttt
    13741 cgatatagtt gatcaaagaa tctttctttt tctggatgtc ttttgaaatg attaattgct
    13801 caaagtcttg accaccgcca aagaaaacca agtcatattt atcagcatca aaaggttcat
    13861 gtatactaac gatctccgta tggcaagaaa cccccatttt ttctgctata tatttcaaca
    13921 tcaaaaggtt tccgttatca ccatatgtgt tcatcaaatt tccataaagg tgggcgatat
    13981 tgagttcata attagccacg gttcattcct cctttgataa atccttgagc agctagttct
    14041 ttacgaagct gcaatacagc agtgtaagta gctaaaatat agacatgctc agttggcaat
    14101 tctttgattt tttcgattac ttctggtaat tcttttgttt ctgtcaattt attttctgga
    14161 atcccggcaa cttttagacg taaagtcata tctgtatgac ggtctcctcc agaaataaca
    14221 gctgggatat ccatatcggc aaagttttca aaattaccat cccaaatcca gctgacatcg
    14281 attccgtctg cgtaattggc atttaataaa gccactaaag aaaatggata aggagctaga
    14341 ccaatcatat cgattacttg attgatacct acaggatttt tgacaagtac taatgtacat
    14401 tttttcccat tgatattgat gacttcttgt cggccgaata ctttctcgtc atatcctaaa
    14461 ccttgtttga ttttatcagg atcgacattg aagtactctg ccactgcagt agctgctaaa
    14521 gcattgtaga cattatacat accgccaact tcgattccgt attcatgacc atcgatcacg
    14581 aaatctgctg acacattgtc catccgtacc atatcagtta atcgataatc caattcagga
    14641 cgtttgaagt cacaatttgg acagtaatat ttccctaagt ttgcataagt gatcatttta
    14701 taatgcaaga tgtgatgaca atttggacat aagacaccgt ctgtattgta atgagccatt
    14761 tgttcacgat cttcttcatg gtcaaagcca taatattttc taggattgac tgtttcgata
    14821 gaattgaaga ttggtgaatc accgttactg ataatcgttg catttggtga ctttgcggca
    14881 ccgtctacaa tcattttata ggtcgtatag atttctccat accgatccat ttgatcacgg
    14941 aaaatatttg taaataagaa cagttctggt ttcatatatt ctgtgacttt gctcaaactt
    15001 gcttcatcta tttctaggac agcaaacttt ttctgtcctt tttttggttt tgcctgcaag
    15061 aaagtcgata caattccctg taccatattc gcaccagtag ggttggttag aacttcatcg
    15121 aattgctggc gcaaaatatt gactgttaaa gcggtagtca atgttttacc gtttgttccg
    15181 gtgacaacga cgacttggta gtcttttgcc aaactatcta gtatatgtgg atcgactttc
    15241 agcgctagtt tacctggtaa acttgatccg cctttaaaaa aagtttttaa tgcccattgc
    15301 gacgtttttc caactgcaat cgcaaaatga ctgcgtattc ccattatgta acctccaaaa
    15361 acgatttgct gacgaataaa ttcaactaat cataccatat gttgaaaaaa aggttaaatt
    15421 gcttttctta ttcttaagaa aaggttacgg ctgaaagtgc atgaaatcgg acaaaatcgc
    15481 atgatttaac aaaaaaacag cttgaaacaa aatgattgtt tcaagctgct gtgattttat
    15541 tattccctct tacctatatt cccattttaa ccgataataa ttttctcgct tggatattca
    15601 tagcctagat ctcgtgtttc ttttggcacg aataacatca atgtatatag cattccgatc
    15661 cgcccaataa acatcagtgt agcaatcgtg attttgccca caacagacaa gtctccggta
    15721 atccctaaag ataaaccagt tgttccaaac gcagaagtta cttcgatgat gatcgagatc
    15781 agcgtttgtt cttctgtagc agataaaaaa acgatacaaa agaaacacat accaagagaa
    15841 agcataaaga caacgactga ttttcgcaca tcatcttgat cgattctccg tccaaaaata
    15901 ttgatgttat cttcactttt gaggaaggaa tatagataca aaccgatgat tgctacagta
    15961 gttgttcgaa tcccgccgcc tacagaacta ggactgcatc cgataaacat cagtaaagaa
    16021 aaaatgatca gtgttgtgat ttgaaaatca cccaaatcgt gaatctgcag accagcatta
    16081 cgagtcgtaa tcgaataaaa catcgaattg atccatttga cactcatatt ggaatcttgg
    16141 aataaatggt ccttttccaa tagataaatc aagacagttc ccgaaacaaa caagataaca
    16201 aaagctaaca cagctaattt ggtaaacaag gaaaaacgaa acggaagctt cgcattggaa
    16261 cgtttgtata atagccattc ccggcactcc atcaatacag gaaaaccaat tcctccgaca
    16321 aaaatcaaaa acatgatcag gatcaagaaa aaatagtcat gtgcaaaggg cttgatggaa
    16381 tccccagtga cgtcaaaccc agagttcgtg acagccgata tcgcctgata aaaaccgtaa
    16441 aaaacggcat cacgccaacg atcaaaatag cctctgtaat aaaaataaat agaaaagaat
    16501 gtcccaaaaa gtaattgaaa ccagattaaa atagcaaaag tgatccgaat cagtcggacg
    16561 ataccagaca aacgcggttg attcatatcc gtcatgatca gctgtcgctg tttcagtgtg
    16621 atccgtcgtt tagaaaaaat gacaaaagca gtagaaatca tcatgatccc caaaccacca
    16681 acttgaaata atatttccag cagtataata ccgttatcat tgaacacaga attgatatca
    16741 aaagtcgata gtccagtcac actcactgta ctgattgcca tgaacagcat gtccacaaaa
    16801 gaaacatggc tatctggttc gcgaaaaatc ggcatataaa atagaacgag tgataagacc
    16861 gtcatcaata tataataaaa aacgatgatt tggatcgatg agaaatgatt ggcagcaaac
    16921 ttctgtcctc gccgctgcag ccttcgaaag aataaatcaa catgcacagt actatcctcc
    16981 tgacaatagt cgtttctacc agtatacaag gggaattagg aatactcaaa gaaattatca
    17041 aacagataac aatacagctt tcgattaaaa tagaaatggc ttataataag gaaaatccta
    17101 gaaaaaagaa ggaggaggaa taacaaatta tttaatgctc aacaaatcgt agaaaaaatg
    17161 aaaaatcaaa aaataaatta tgatacagtt ctgaaaaaaa tgatcatgca atgggagaaa
    17221 gaagaagtaa agcctaagat attaatacat acatgctgtg ctccttgcag tacacacaca
    17281 ttggaattca tgtctcaata tgcagagatc acgttatttt tttcaaattc gaatattcat
    17341 cctaaaagtg agtatttgcg aagattacat gaacaaaaac gatttgtaga ccaatttaat
    17401 gagaaaactg accatcatgt tgcgctaatc gttgatgaat accgtccaaa tgaattcacc
    17461 aaaatggttt taacaaacca tcttgaaaac gaaaaagaag gtggtgctcg ctgcagtgca
    17521 tgcttcaata tgcgattaga tcttgttgcg caaaaagcgc aagaactagg atacgattac
    17581 tttggaagtg ccctgaccat ctcgccaaag aaaaatgcag ctttgatcaa tcagatcgga
    17641 atggatatcc aaaagattta cgggacacaa tacttgccaa gtgatttcaa gaaaaacaaa
    17701 ggctatgaac gctctttgca aatgtgcaaa gagtatgata tttatcgcca atgttattgt
    17761 ggttgcgtgt tcgctgccaa gcagcaagga tgcgatctga aagaaatcaa tcgggaagca
    17821 aaagcttact taaaaacatc aaccgtagtt tttgaggaaa attaaagaaa tctttcggaa
    17881 aaacgtaaga aaaaaataaa gaatttatgg tagaaataag gtagactgta taatttgtcc
    17941 gtagtaaagt aaggaaacaa gaataaattt caatgaagga agagataaga tgcgaaaagt
    18001 agcacctatt ttgaagccaa tcgctgtatt aacaatctcg acgattgtcg cgactactac
    18061 aggtattgtg aatagttcca gctaccgtgc accaagttca agttctagta ctgtagaaga
    18121 aacaacacca actacttcag tctcgacatc tgaaccaaca acaacggaaa gtttgaatga
    18181 aaccactacg acaagtacat gggacgatac aacaactagt tcgtcgtatg atgagacata
    18241 tacaactgac agctcaagcg aaacaagtaa tacggtacca gatggcactc catcggattc
    18301 aaccaatcca agtacaagtc cggatacaag tgctagctcc gaaacgatac caagcagtac
    18361 gactgtagat gataacacgc aaaatgatga tgcagcacgt acagcacagc aaaaccaaga
    18421 ttcaggaaat acccaaacgt ataaccagca agcaggtgat tctcaatgac atttttacta
    18481 gaccttatca acggggtgca taaattcctt ggctatttag acatcagtcc taaatatctt
    18541 aaccgtggat atacgatttt aagtgtgatt cccacgcttt atttattgag aattgtttat
    18601 ggactttggc aaaaccaaaa ctatcttcaa tttttcttat atggacttgc atttctagtg
    18661 cttgtttatt ttactgtttt aaatgtattt tattactttt tagataaaaa cacgaaagcc
    18721 gatgtcactc agctttttgt gaagtattta cctgatgaag cgttcaacat ccagacagaa
    18781 gaatcgatag ataatggaaa ttcgatcgat aaagtgaata cacaagaggt catggtttct
    18841 tatgacgaag attatcaatt gaaattagcc gagaatatgc ggtatttgat tggaaatggt
    18901 gagatcaaaa caaatgaact cggtcgcatg gatggatttc tggtcgatag aaatacattg
    18961 tatccttatt actatgtcaa gaaaacagga gagaaagagt atgttctaaa tattggacga
    19021 agctactcgg acatggaaaa agtagggacc attcgacttg actcaacaga agacacattg
    19081 gaacctgtcg gattgttcat cgtcggcggt gattttgtca aagacgggtt acgctatcat
    19141 gagccttatc gtctgaaatt gattgcaaaa aaggcacagt cgcaggaaga accagtggtt
    19201 tatagtcgta gccaaagaag aagacattca taatgtgatg aaacgccgtg tatcagtggt
    19261 tgcgataaaa acgtttattc ggaataaggg ggatgtgaca cgtcttttgt cacgttcctt
    19321 ttttatataa cgagaaagat aagacgaaca catcacttat tctttccttt catttataaa
    19381 cattgtagaa taaagaaagt acaacgaagt attgtgaaaa aacgtaataa ataacgagga
    19441 aaatacaagt aggaggtaag ctgtgaaaga atttttgaaa aattggggga ttttgatcct
    19501 tgtattagcg gctattttat tagctagagt gtacgtgttt acaccagtta ctgtaaacgg
    19561 gcattcgatg gaccctacat taagtgatgg acaacgattg atttcatcta agatctcaaa
    19621 ctatgaacga atggatatta ttactacaaa ggaaccgggg gatgaagaac ggatgattgt
    19681 taaacggatc atcggtatgc ctggagatac agtcaagatg gaaaatgacc agttgaccat
    19741 taatggcaaa aagtatgatg aaccttatct ggatgagttc aaaaaagaat tttcagaaga
    19801 taagttacaa ggagaatatg cttatagctc aggattccaa gcacaagcag aaagttcatc
    19861 cacctttacg agtgactttg aatatactgt gccaaaagga aagtatcttg ttctaggcga
    19921 taaccgcttg atttcaaaag acagccgaat gtttgggtta gtcgacaaag atatgatcca
    19981 aggaaaagtt gttttccgtt attggcctct ttcagaaatt aaaatcatgt gatgaaaaaa
    20041 taaaaagaag ctgtgacatg ttttttgtca cagcttcttt ttccgaaaat tccttcttat
    20101 ttcttggagt tgctcacttt ttttgcaatc tcattttaag ctttttggct gatggataag
    20161 aaaagcgaag cgtttttgtt cataaatagc ttttccccac gatggaaggt atgactagta
    20221 gcagagacaa agcccgtttt ttccaatcga ttattcaatt cttcttggct aaagccatta
    20281 tggactttcg gatgactaat ttgttcattc ttgtcaaagt ctacgatgat caattgaccg
    20341 ccaggagcta aaatttcata aagttttgta agaatattct ccgtatcagg aatgtgtaaa
    20401 agtacaagtg atagaaggat gagattagct tggacagcag gaaattcgac agaagcatca
    20461 gcgtggatcg ttcggacatt tttcagatct gctgcttgga tcttttcttc tgccattttc
    20521 agcatagttt ctgaagcatc ggcaatgatc agttctttga atctttccgc aagcggtaaa
    20581 ctaacaagcc cagttccgcc tccgtaatcc agcaggactt tttggtggct gtcgcgcggt
    20641 agataagtag ttagttcttc gcgaataatc ttggctaatg cttgacggtc aggggaatca
    20701 taatggttag cgatggaatc aaaaatattt tttgtcatca aatagctcct tacgtaaaac
    20761 tttgaaattt taacttatta tagcacgtga aaagaaagaa agcatgctat actcaattca
    20821 aaaagggaga atctagaaag aatatgccga tatcgtttgt aaaagataga gaggaaaaag
    20881 gaaagtgtgt ccgagaaata ttactagact tgcctgagtg gtttgggctt cctgaatcaa
    20941 cggaaaaata tatcgaggaa tcaagtaaac tacctttatg gtgtgaaaaa agaaaagaag
    21001 aatatttagg ctttatcact ctttcacaga caagtgaaga tactgccgaa atctattcta
    21061 ttgtatggga ataaaaaaag actatcatca ccaaggatta ggaacaaaac tttttaaaga
    21121 agcagaaggt tacgcagcaa aacattacaa atatttgcaa gtgaagactg tagacgaggg
    21181 gcactactct atatatgatc aaactatttg tttctacgaa tcattaggat tttcaaggtt
    21241 ggaagttttt ccaaatttat gggatgaatg gaacccatgt ttagttcttg tcaaaaaatt
    21301 ggaacaaaag tgagcaatag atttaatact aatctgtgct tgatgaaaat tctcactgta
    21361 aaaaatgctg aaaatcaagt ataataccat aggaacaaac tggaaggtga aaaagagcat
    21421 ggcacaacta ttttttaagt atggcgcaat gaatagcggc aagacgatcg agatcctaaa
    21481 agtcgcacat aattatgaag aacaaaataa gccagttgtc ttaatgacaa gcggtattga
    21541 tacaagagat ggtttaggcg ttgtttcaag ccgaatcgga ctgaaacgta atgcgatgcc
    21601 gatatttgaa gaaacagacg tatttgacgt aattcagaaa atggaaagca aaccctattg
    21661 tgtgctgatc gatgaagcgc aattcttaaa aaagaaacat gtcattgctt ttacgagaat
    21721 tgtggacgaa ttggatattc cggttatggc atttggtttg aaaaatgatt ttcgaaacga
    21781 gttatttgaa ggatcaaaat atttattgct ttatgcagac aaaatcgaag aaatgaaaac
    21841 aatctgttgg ttctgccaca aaaaagcaat catgaattta cattatattg atggaaaccc
    21901 tgtttatgaa ggcgatcaag tgcaaattgg cggaaatgaa gcttattacc ccgtttgtcg
    21961 aaaacattac tttcacccat caatgattac agaaggagaa taagcatgta cgatcaatta
    22021 caagcaattg aagaccgtta tgaagagctt ggagaacttt taagtgaccc agaagtgatt
    22081 tctgatacaa agcgttttat gcagctttca aaagaagaag caaacacgcg cgaaacagtc
    22141 gaagtttatc gtcgttataa agaagtagta caaggaatca ccgatacaga agaactgctt
    22201 ggtgaaaaac tggatgatga aatggctgaa ctagcaaaag aagaattatc tgaattaaaa
    22261 aaagaaaaag aagtcttaga agaaaaaatc aagatcttgc ttttaccaaa agatccaaat
    22321 gacgataaaa atatcatcat ggaaatccgt ggagcagcag gcggagacga agcagcatta
    22381 ttcgctggcg atcttttcaa tatgtaccaa aagtatgctg aagcacaagg ctggaaaaca
    22441 gaagtgatgg aagctaacgt taccggaatc ggtggatata aagaagtgat catgatgatc
    22501 accggagaca acgtatattc gaaattaaaa tatgaaagtg gtgcgcaccg cgtccagcgt
    22561 gttccttcaa ctgaatcgca agggcgtatc catacttcca ccgcaacagt tgtcgtgatg
    22621 ccagaagctg aagaagtaga gatcgatatt gaagacaaag atatccgtac agatatctat
    22681 catgcttcag gtgctggcgg acagcacgtc aataaaacag catctgctgt acgtttgact
    22741 cacttaccaa cagggatcgt tgttgctatg caagatgaac gttctcaaat caagaaccgt
    22801 gaaaaagcaa tgaaaatctt gcgtgctcgt gtgtacgata agatccagca agaagcacaa
    22861 agcgagtatg atgccaatcg aaaatcagca gtcggaactg gggatcgttc agaacggatc
    22921 cggacgtata atttcccgca aaatcgtgtg actgatcatc gtatcggatt aactatccaa
    22981 aaattagatc agattttagc agggaaatta gatgagatcg ttgatgcatt gatcatgtat
    23041 gatcaaacat caaaactgga agctatgcaa aatgggtaaa atgacctatc gagaagtcct
    23101 gtcacgggct tcttcttttt tagaggatca tgggaaagaa gcatatagta tccaattttt
    23161 atttttggaa cgaaaacaat ggaaaaaact tgattggctc cttcatatga acgaagagat
    23221 ttcagaagaa gaacagcggt tgatcgaaac cgatctgcga cttctgcttg cagatcatcc
    23281 acctcagtat ttgctaggat atgcagattt ttatgatcat cggttaaagg taactgaagc
    23341 aacactgatc cctcgaccag aaacagagga gttagtcgaa tggtgtttag acgagacgcc
    23401 tggtgtaccg ttagaagtga tagatattgg gacagggact ggagcaattg ctattagttt
    23461 gaaagcagca agaaaaaatt ggcatatatc tgctgtggat ctttcagaag aagcattaga
    23521 agtagcgaaa gaaaatgctc agaaagaagg aacgaaaatc agtttttatc atggagatac
    23581 attaggacca gtaatggatc agcaatttga tgtgattatt tctaatccgc cgtacatcag
    23641 tcggaatgaa tgggaactga tggatgaaag tgtccggtca ttcgaaccta aaatggccct
    23701 ttttgctgag aacgatggat tagctattta tgaaaaaata gcagttgaag caccaagcgt
    23761 actaaaatca aatggaaaga tttttttaga aatcggattt cgccaaggtg aagcagtaaa
    23821 aaaaatattt cagcaagcat ttccagataa aaaagtggcg ataaaaaagg atctttttgg
    23881 caatgaacga atgatccaag tttatggttg aagttgtgga tgaaaaattt tttatttgtt
    23941 tttttattat aaaaaaacac ttatttgaca aggatgtaaa cgactttttg gtttatcaac
    24001 aagtttatcc actattgaca aatagattat ccacaaacag tggaaaactt gaggataaac
    24061 tcagacaaaa taagaatgta tgaaaaaggg gttgtggata aactgtggaa acgaaacgat
    24121 atgatgaaac agaattgcaa gaggcagccg aagcactgaa agaaggagaa cttgttgctt
    24181 ttcctaccga aacagtatac ggattgggcg ccaacgctct tttaccaaac gctgtcaaga
    24241 aagtgttttc tgtaaaaggg agaccgcaag ataatccatt aatcgtccat gtcgcctctt
    24301 ttgaacaagt aaaagagtat gtggacaact ttcatccggc agcagagaag attgtgaaga
    24361 acttctggcc tggaccatta actttgatat ttaagatcaa aaaagacact ttgccctcag
    24421 ttgtgacggg aggattatcc acagctgcat ttcgtatacc ggataacaaa aaaacactag
    24481 aagtaatcga actttccggt gttccgcttg tcggtccaag tgcaaatacg tcgggaaaac
    24541 caagtccaac gactgcagat catgtatatc atgatctaca aggaaaaatt accgggatta
    24601 ttgatgatgg agctacgcga attggtgtag aatccactgt tcttgattta agtgatccaa
    24661 cagccatgcc tatgatttta cgaccaggtg cagtaacgaa agaacaaatc gaagcagtca
    24721 tcgaatctcc tgttgctatt gatcagcatc ttgtaaaaga aaatgaaaca ccaaaagccc
    24781 ctgggatgaa atacaaacac tactcacccg atacacgtgt tctgatggtc agagatggcg
    24841 attggtcaac tgcagttcaa tgggcaaaaa ataagaagat ccgtgcaggt gtcatagcaa
    24901 gccccgaaat tgcggatcaa gtgcggacag atactgcagc tgtttatatg tataacgaca
    24961 actcagtaga agcggcggct aaaggattgt ttgcaggtct aagaggatta gatgaaccaa
    25021 cacttggatt agatttgatt tttgtacaag tgtatccaga aactggattg gggaatgctt
    25081 acatgaatcg cttgaaaaaa gcagcaggac aaaattattt tgaaaaatga tcaattggtt
    25141 attgtcgcat tcttttagta tgatacaatg ctatcaagta aatcttctat cagtgaacga
    25201 aaaactaagc gtaactaatc gtaatagaag agatacgaat atttggcaat ttgtttttga
    25261 tgggaaaaat aaaattgtag gaaaataaaa aggagctgtt gtttgtggta gattacaaaa
    25321 cgtttgaccc tgatctttgg gctgcgatcg ccaaagaaga agaaagacaa gaacataatt
    25381 tggagctgat tgcctctgaa aactttgtct ccgaagcagt gatggctgca caaggaagta
    25441 tcttgacaaa taaatacgca gaaggttatc cagggcatcg ctattatggt gggtgtgaat
    25501 ttgtcgatat cgttgaaaat ttagcgattg accgtgcaaa agaattattc ggagcgaaat
    25561 ttgcgaacgt ccagcctcac tccggctctc aagccaacac ggctgcttat ctcgctttgg
    25621 tagaaccagg ggacacgatc ttaggaatgg atctttccgc aggcggacat ctaacacatg
    25681 gatcaccagt caacttcagt ggcaaaacct atcactttgt tgcttacggt gttgatccga
    25741 caacagaagt tattgattat aatgtggtac gtattttagc tagaaaacat caaccaaaat
    25801 tgatcgtagc aggtgccagt gcttacggac gaacgatcga ctttgcgaaa ttcagagaaa
    25861 tcgcagatga agtaggcgca aaactgatgg tagatatggc acatatcgca ggattagtag
    25921 cagcaggact tcatcctaat ccagttcctt atgcagatat cacaacgact accactcata
    25981 aaacattgcg tggccctcga ggcggcatga ttttgacaaa cgatgaagca ttagcaaaaa
    26041 aaataaatag tgcggtattt cctggtattc aaggcggccc tctagagcat gtgattgcgg
    26101 gtaaagcagt agcattcaaa gaagcattag atcctgcatt taaagaatat agtgaacaaa
    26161 tcatcgccaa tgcaaaagca atggtcaaag ttttcaatca agcaattgga accagagtca
    26221 tttcaggggc gacagataat catctgatgt tgatcgatgt tcgcgagtta ggtataaatg
    26281 gaaaagaagc agagagcatt ttggacagtg tgaatatcac tgtaaataaa aactcgattc
    26341 cttttgaaac actaagtcca ttcaaaacaa gcggtattcg aatcggcacg ccagcaatca
    26401 ctactcgcgg gttcaaagaa gaagatgctg taaaagtggc tgaactagtt gtcaaagcat
    26461 tacaagcaaa agatgataac gctcaactag acgaagtcaa aacaggtgta cgcgaattaa
    26521 ctgaaaaatt tccgttgcat aaaaaataaa aatttaaatt ccattagtaa acagaaaagt
    26581 tcaatgttgg gcttttctgt ttattttttg ggaaaacgga ttctttgaca atatcggtga
    26641 tgctctctag tatatgaggt gtaattttag tcttcacact aaatctcagg gaggaaaagt
    26701 catgactgat cgtatctcgt tagaaaagga agcagtagca ttcagtgatg caaatgcgcc
    26761 tcatccgcgt atctatgaac tagcaccgga agaaggaaga gttttactag aagaagtaca
    26821 agaatcacct gtgaacaaat attccgtcga tattgaagac ttgatggtgg atactggcga
    26881 atggggaaaa ctcaatattc gtctcgtacg accagaaggg aatatggata aattaccagt
    26941 tattttttat attcatggag caggctgggt ttttggcagt gctcaaaccc atgataaatt
    27001 gatcagagaa cttgcggttc gtacgaattc cgtcgttgtc tttccagaat atagtcgatc
    27061 accagaagca aaatatccaa cagcaattga acaaaattat gctgtgttgc aacagctgaa
    27121 taaattagcc aagtcaaaaa atctggatct cgagaatcta atcgttgcag gagattcagt
    27181 aggtggtaac atggctacag tcatgacgat cttaacaaaa gaacgaaagg gcctcccaat
    27241 atccaagcaa ttgctgtatt atccagtcac aaacgctgat tttgatacag attcctatca
    27301 tgaattcgga gaaaactatt ttttgacaag agaaggaatg atgtggtttt gggaccaata
    27361 tacgacgaat gaagaagagc gtgctgaaat cacagcttcg cctttgagag caaatataaa
    27421 tgaactaaat gatcttcctt cagcaatgat tttaacggga gaagcggatg tattaagaga
    27481 tgaaggtgag gcttatgcgc gcaaacttcg tgatgcaggg gttcctgtta cacaagtacg
    27541 cttccaagga atcatccatg attttgtcat ggtaaatgct atggatcaga caaaggcaac
    27601 gcgtgcagca atggcattat ctacttcttg gatcaagaaa aaagaataag ctttgcttgt
    27661 ccgcaggaaa acttcttttc aaaaacggat tttcttagga aaaatggcga gtttgactag
    27721 tcaatcactt gattttgttt tacaattaaa aagaaacgac agaacaaagg agagcttaaa
    27781 aaatgggcaa atttcaagtg atcgatcatc cattgattca acacaaacta actattattc
    27841 gggacaaaaa ctgcggaaca aaggtttttc gtgaagtagt cgatgaaatc gctatgttga
    27901 tggcatatga agtatcacgg gatatgccat tggaagacat cgtgatcgaa acaccgatca
    27961 ctgaaacaac acaaaaaacg ttgtctggta aaaaagtagc gattattcca atcttgcgcg
    28021 caggtattgg aatggttgac ggtatcttgg aattgattcc agctgctaaa gtgggacata
    28081 tcggtcttta tcgagatgaa gaaacattgg aaccacatga atatttcgtc aaacttccag
    28141 aagacattga tgcacgtcag ttgtttgtag tagatccaat gttggcaaca ggcggatcag
    28201 caatcatggc aatcgatgca ttgaaagaac gtggcgcgtc gaacattaaa tttgtttgtt
    28261 tggtagcagc acctgaggga gtaaaagctt tgcaagaagc gcatcctgat attgacatct
    28321 atacagcttc tctagatgaa aaattgaatg aacatggtta tatcgttcct ggactagggg
    28381 atgcgggtga ccgtttgttt ggtacaaaat aagtaaatac gaaaaagggt gtgacacaac
    28441 ttttgtcaca tccttttttg gtcattttta acgtttgatg cagaacagct ttttcacagc
    28501 tttcgtttgc atataaaaaa accatcccaa aaatgcagag atggtcaaat tcaaatcttt
    28561 tttagttctt tgtagttaac gattactaca cccttacttt gcgtcatcaa gttcgcattt
    28621 tctgcagttt catcaatctc aacaacagcg gaatttttca attgctttga aatcgttcca
    28681 gtttgcgact tgccgtgaat tgtgaacatt acgtgagtat tgatcttgaa tttttcacca
    28741 atttccgatg gtacgatagt tgtatcaaat tttccaaaag ttgccatttt cataaaacct
    28801 gcctttcaca tcaattatac cacgttttat gaaaaatatc atgaaattaa actaattttc
    28861 catcggactc tttacaatca gccaaataga gtatataatt tcttttggat aaggccagct
    28921 tagctcagtt ggtagagcaa cgcactcgta acgcgtaggt cgtaggttcg atccctatag
    28981 ctggcattaa aagagaagac agatatatca aggattttta atccattgat acatctgtct
    29041 ttttgtttaa aggaaataaa gtatgctttc ttagcaatag agcatgtatt atttctataa
    29101 aaatttagga gaataaaacg actagcaata tagctgtgaa atcaaatatc acatgaactg
    29161 cccaacttgt aatgatcgag ttgcttttaa tggcagcttg gttgaataac aatcttgcca
    29221 ccccttgtat caaaaggatg tgtgctacag tgtgtaaaat atttccatta tcataagtac
    29281 tgtaatggat aaatccaaag atgattgctg acaagaaact agcgacagga atgctgaact
    29341 tactagaaaa aatagcgaga aaatacactg agaacaattc ttctccaagt atcatgatag
    29401 gcaaaataat aaataataac gggctgaaag cctcatttat tggattgcct gttaaacccc
    29461 atttcaaaat ttgttgcaaa aataccgaga ctgcggcact gattactaca ttgataaaaa
    29521 aatatttggc tatatttttg acaggagcga caggtttcga gaacattttt ttcgtatctt
    29581 ttttaccaaa aacaagtgta aatgctataa atccagtgat agttaaagaa aaaatgctaa
    29641 taatggatgg cgcaaatagc aaccctaaca aaccgagtac aacgacaata gtgcccaata
    29701 tccctttttt aagttctatt ttttttccta aaatgttatt catgataata actcctacta
    29761 ttcagttttt gaagatagag tcagtataca tgaagagtta aaataggcaa tgttttttta
    29821 tggatatttg tattgctaaa aatttatctt atatattcat tttgattata ctacttttat
    29881 ttgtaaaaat tttctagcaa aaccaagtgc ttgcaactga ttaagcgtac aattttttag
    29941 atgttccgag cgtacttcaa tcatttcaaa ggagttgcta cttaaatcga tattttttaa
    30001 atcagtatca aaaaagctag cattttctaa atcattagta gaaaacatta ttttagtgag
    30061 ggaacagtct acaaaatctg cgcttgataa gacagagtgc tcaaaggtac tttcttttat
    30121 gtttgaacca gataaattta catagtccat tttacaattc tcgaataacg tgttcccaat
    30181 ataactttcc atcaatttag tacctgttaa tttacaattg acaaatttca cgcgatgcaa
    30241 catgcttttt gtcaatatac agcctgaaaa atcacagttt tcgaatatac agtcggtaat
    30301 ctccattctt tgaaaatcat tggaagcaaa aaatgcatac ttgaaaatag atttgtaaat
    30361 aattggatgg cagattattt ggttttcgat gacctctttt tgtgaaagga tattatacag
    30421 ttcaaaatct tcgttttcat agatatgaaa aaaggatcct ttattcaagc ttgcttcatt
    30481 aactttagga aaaatcatat ttaattacct ccattttcat aaagtcatat taacaaaagc
    30541 taaaccaact tacattagca gaaaatgaga taggttttcg cctttttcga taacgatagt
    30601 gtcgctatct cttgaaacgt tcttagaatt gccctgagag acacaaatta ggcgtaattc
    30661 taataaaaat cttagaataa aaatttttac tgatttaatt tagaatgttt ttaaaataaa
    30721 aatcgttgcc ataacagtgt attgaaaggt tttaacaatc cagtaatgat ttaaaaaaat
    30781 ctagatgtag cgtatacttt gtttatagaa tgtagtggga agaaatatat ttttgggagg
    30841 aaatcgtgat gtctaaaagt gaagaaatag aaaacatggt actaaaatta tatgatctcg
    30901 ttttaaatcc agagatcaaa gaaaaggaac gtatactttt gatagacgca aagacaggct
    30961 tagaaaaagg tcagtattat cctaaagtca taaataattt ggaacgtagt ttacgtccgt
    31021 tagcaatcag gggagaattg tctaaaccag tatcaccgtt ttatatggag atatcaacaa
    31081 taggaaaatt tgaaaaagaa ttaggtcgag gaatggcttc ggcaccgatt acttttgggc
    31141 atttataaaa acgagttatt catctcaatg aattaaaaag tatagtcaat ctcataatgc
    31201 aggctaggtt catttatttg aacagagtct gtattttttt attctaaata aagaaacaga
    31261 tgtttcttta tattgtattt tatcctttaa ttaacacaaa aacagctagt ataaactaat
    31321 aatgttaatt aaaagtgtat tttatgaaac t
//
LOCUS       DAIGOK010000020        31176 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.020, whole genome shotgun sequence.
ACCESSION   DAIGOK010000020 DAIGOK010000000
VERSION     DAIGOK010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31176
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.020"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            44..361
                     /locus_tag="NOA01_000702"
     CDS             44..361
                     /locus_tag="NOA01_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCI0631922.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            354..962
                     /locus_tag="NOA01_000703"
     CDS             354..962
                     /locus_tag="NOA01_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HCI0631923.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            1017..1808
                     /locus_tag="NOA01_000704"
     CDS             1017..1808
                     /locus_tag="NOA01_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HCI0631924.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            1946..2434
                     /locus_tag="NOA01_000705"
     CDS             1946..2434
                     /locus_tag="NOA01_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HCI0631925.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            2485..3357
                     /locus_tag="NOA01_000706"
     CDS             2485..3357
                     /locus_tag="NOA01_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HCI0631926.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            3533..3895
                     /locus_tag="NOA01_000707"
     CDS             3533..3895
                     /locus_tag="NOA01_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCI0631927.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            3904..4656
                     /locus_tag="NOA01_000708"
     CDS             3904..4656
                     /locus_tag="NOA01_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0631928.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            4670..6472
                     /locus_tag="NOA01_000709"
     CDS             4670..6472
                     /locus_tag="NOA01_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI0631929.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            6749..7789
                     /locus_tag="NOA01_000710"
     CDS             6749..7789
                     /locus_tag="NOA01_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCI0631930.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(7841..9985)
                     /locus_tag="NOA01_000711"
     CDS             complement(7841..9985)
                     /locus_tag="NOA01_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCI0631931.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(10342..11715)
                     /locus_tag="NOA01_000712"
     CDS             complement(10342..11715)
                     /locus_tag="NOA01_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCI0631932.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(11715..12110)
                     /gene="rbsD"
                     /locus_tag="NOA01_000713"
     CDS             complement(11715..12110)
                     /gene="rbsD"
                     /locus_tag="NOA01_000713"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCI0631933.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(12138..13055)
                     /gene="rbsK"
                     /locus_tag="NOA01_000714"
     CDS             complement(12138..13055)
                     /gene="rbsK"
                     /locus_tag="NOA01_000714"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCI0631934.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDALDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(13163..14176)
                     /locus_tag="NOA01_000715"
     CDS             complement(13163..14176)
                     /locus_tag="NOA01_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCI0631935.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(14257..14856)
                     /locus_tag="NOA01_000716"
     CDS             complement(14257..14856)
                     /locus_tag="NOA01_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCI0631936.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            15042..15743
                     /locus_tag="NOA01_000717"
     CDS             15042..15743
                     /locus_tag="NOA01_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0631937.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            15753..18464
                     /locus_tag="NOA01_000718"
     CDS             15753..18464
                     /locus_tag="NOA01_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI0631938.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            18915..20543
                     /gene="treC"
                     /locus_tag="NOA01_000719"
     CDS             18915..20543
                     /gene="treC"
                     /locus_tag="NOA01_000719"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCI0631939.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(20603..21784)
                     /locus_tag="NOA01_000720"
     CDS             complement(20603..21784)
                     /locus_tag="NOA01_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCI0631940.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            22036..23559
                     /locus_tag="NOA01_000721"
     CDS             22036..23559
                     /locus_tag="NOA01_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HCI0631941.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(23628..24356)
                     /locus_tag="NOA01_000722"
     CDS             complement(23628..24356)
                     /locus_tag="NOA01_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCI0631942.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(24369..25649)
                     /locus_tag="NOA01_000723"
     CDS             complement(24369..25649)
                     /locus_tag="NOA01_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCI0631943.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(25790..26392)
                     /locus_tag="NOA01_000724"
     CDS             complement(25790..26392)
                     /locus_tag="NOA01_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HCI0631944.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            26637..26843
                     /locus_tag="NOA01_000725"
     CDS             26637..26843
                     /locus_tag="NOA01_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631945.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            27155..27874
                     /locus_tag="NOA01_000726"
     CDS             27155..27874
                     /locus_tag="NOA01_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCI0631946.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(27955..28638)
                     /locus_tag="NOA01_000727"
     CDS             complement(27955..28638)
                     /locus_tag="NOA01_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HCI0631947.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            28856..31042
                     /locus_tag="NOA01_000728"
     CDS             28856..31042
                     /locus_tag="NOA01_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCI0631948.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT        10402 a         5494 c         6242 g         9038 t
ORIGIN      
        1 agattgaaat aaaatattac acttgagaat agaaggtgac gtaatgcaca tccagattcc
       61 aactgttctt cttgatggta ctgtgctagc agcattaagc aaggaagata tgtatgggta
      121 tgccttaaca aaaatcgtac aagaaagtct ctctgtcagt gaatctacac tatatccggt
      181 tttgagaaga ttgaaaaaag aaggatatct tgagacctat gatgaacctt ttgaaggcag
      241 aaaccgtcga tattactcgt tgacaccaat gggaagtcgt catttagaag aaattattga
      301 tgaatggatt gtttttcgag attctttaga ccggttattg gaggagagca caaatgaatg
      361 aatatttgaa tgaagttgtg gatcatcttc aagaaattcc tgaagacgat cgttttgatt
      421 tgatccagta ttatgaggaa tattttttag attctggaaa aacattagat cagatcattg
      481 atgaatatgg tacaccaaag cagtttgcct taaaattaaa aatcagttac ttttcagaag
      541 aagaacaagc agaaaacgag ttagtgaaaa atccaaaaag acaaattcgc ctgatttggt
      601 taatcattat cggtttattt gcttctcctg tcctcattcc actagcactg gggtttatat
      661 tggttattgg gggactattg gttgctttac tagcgctcat ttttagtatc tatgttttat
      721 gtatcagtat cttaggtgtt ggcctattca tgatcatctc aggaatcagt gtgtttggac
      781 aatcgattct gagtggatta ttctttatcg gtgtcggtct gttcgccaca ggaagcgcag
      841 tatttatttc gccactgttg ctgaaaggaa caaaagggct tttttatgtc atgatgaact
      901 ttgttaaatg gatgggacga cgttttatta caaaaagaga tctgcattca gcgggcgttt
      961 gaaggaggac acataaattg agattgaaac attttttgat taccggtatt ggtctgatgc
     1021 ttactggtgg cctcctagca ggtgcaactt atacattagg cgctcaaaag agtctgactt
     1081 gggataatgg tcctcggttg atcgatatgg ttactgagac aaaaaaaatc aaggatgcaa
     1141 acaaaatcgt catccatgca gaaaatcaaa cagtttcggt catacgcgga ttggattttg
     1201 agataaaaac gacttatgat aagacagaaa agccagaaat aaaggagaaa aatggcgtgt
     1261 tgactgtgaa tgcagtcaaa aaatcaacag gtgcaatcat tagtttagaa gacacaagtg
     1321 cacaaatcac tattgttgtc cctgcagatc atgaattatc tgaattgaca atcgaaggga
     1381 ataatagtgc aattgatatc aacgaagtat caatcaaaaa agtgaatact aagacacatg
     1441 atggctgggt atcgttcaat gaatttacag gaggaacaat aactgcgaaa gatgaattag
     1501 gctcacttcg attagatgag gccaatgtgg ataacttgat cgtcacaggt gaaaacagca
     1561 acttccatct agaagacagt ttgatcagca aagaaggaaa agtcatccta aacggtggca
     1621 atttatcttt gcatgagatg gaaaacagtg gctatactat ccaaacgtct ggtgaatctt
     1681 atattgaaaa agactaccag ccaataacaa atactttgtt agaacaagga cagaaaaagc
     1741 taaagatcac tgctaaagac acatcgatca atattacgac ggatcaagag gacaatgaaa
     1801 cagattgatc ctgtactcaa ctggatattt taagaaaaaa cagcaaagat ccttccaatc
     1861 tttgctgttt ttcatcgctt aacgcacata actatagtaa aataagaaag aaaatatcta
     1921 aaaagaaaac agggtgaaag agtatatgaa ggtagaagaa ggtcaaagag aaaagttaaa
     1981 aacagaaatg gaaagattac atacttatat cactcaacta agccaaacct tctatgatcc
     2041 agacaaagaa aaagtgatgg ttaattaccc gaacaattca gaaggtcgcc aattagagca
     2101 agtgtaccat gaagttttca aacatttatt gacggtgaag aaagagctgg attattattc
     2161 tttaccgatt atcgatacag gaatattgaa gtatgatggg aagaaggaac gatttatctt
     2221 taaatctgtt cgggaagatt tgcctctttc agcagggatg gatctagagg tcttggtaga
     2281 agactatttt acagaagaaa agcactgggt tcgaacaaaa ttggattatt taccgcaggc
     2341 agccggaggg acacaggcaa gcggttggta catcactgaa gacaaagagt tagaactaga
     2401 aggtgtaatg gcgcgtattc gaaaaaaaaa ttaaaaaagt aacattagat atttattgaa
     2461 ggataaagga gcgcaggaag tagcatgaga gaaaaaagag cattttatat caatggttat
     2521 gcagggattg tcggattggt cgtattagct ttagttggat tgtttttctt ctatttaggt
     2581 atgtggcaag cgaaagtttg ggcacttttt ttaagtattt ttctttggtt gatcacgtta
     2641 ttgttgttaa gttcggcaac tgtagtcagt cctaatcaag caaaagtaat tttgtttttt
     2701 ggacaatatt taggtacgat tcgagaaaat ggctttttcc ttacgatacc tttggcacaa
     2761 aaaatgactg tttcgttgaa agttcggaat ttcaatagtt ctgttttaaa agtcaatgat
     2821 ttggatggaa accccatcga aatctcagca gtagtagtct ttaaagtcgt tgatacagca
     2881 aaggcattat tcgatgtagc gtattatcaa gattttgtag aaatccaaag tgagacagcc
     2941 attcggcata tcgctagcca atacccgtat gatacattca atgagaatga tttgacgctt
     3001 cgaggaaaca cgacggcagt gtctgacgag ttgcaaaaag agctgcaaga gcgtttagca
     3061 gtagcaggtg tcgaagtaat cgaaacacgc ttgaaccatt tagcctatgc aacagaaatc
     3121 gctagtgcca tgctccaacg tcaacaagca aaagccattt tgtctgctcg acaaaccatt
     3181 gttgaaggag ctgtcacaat cactcaaatg gccttagagc aaatcgaaga tggacaagaa
     3241 atcaatttca ccgatgatcg gaaagtccaa ttgatcaata atttacttgt ctcaatcatt
     3301 acagacaaag gaacgcaacc tgttatcaat acaggcgaca tgaaagaccg taagtgacat
     3361 actttttgta agataggaag aaggagttta ggacggatta ttgtcttgaa ctcttttttt
     3421 agttgtttta aagaaaatga caaaattgaa agaagaatga tagttgattc gaaggcagaa
     3481 ttgttcgctt ttttctataa tcagtatata aagtaagaga gaaggggaaa aagtgaaaaa
     3541 acttctgatc ggtctacttg ttttcatcgt tgtggtactt gcaggggtga aaatcgcaga
     3601 ttacgtagtt atgggtggag aagaatacta tgtacaaatt acgacagatg gtacaaaaac
     3661 agtttcaaaa tcagactctg gacaagaaac agtcaactat agatatatcc tgcctggcta
     3721 tacgaaagag ggtaaggaaa aagaactgga cttcaaggga caattgactc gaccgcttcg
     3781 taaaggagca tatttaaaaa tcacctggaa caaaaataaa ggtgtcactt cgtatgaaga
     3841 agtcgaaaaa aaagacgtgc cgaaaacagc actggaaaaa ctaattaaag ggtagggaaa
     3901 gaaatgaaca aaatagtcga agtacagaat gtaacaaaag tatacggaaa aaacaatgag
     3961 aaaaagacgc aagcattaag cggcatcagc ttcgatgtag aaaaaggcga atttattggg
     4021 atcatgggcg cttctggttc tggtaagtcg acacttttga atattttatc cacattagat
     4081 aaaccaacag atgggcatat ccgaatcaat caaaaagatg tgaccacatt aaaaggaaat
     4141 caactagcag attttcgtgc gaatgagatt ggctttattt tccaagattt caatttatta
     4201 gaaagcttga cagcacaaga aaatatcgct gttcctcttt ctttacaagg cgtcagacca
     4261 aaagaaatca agcaacgtgt ccaaaaaatt gcagaacgtt tatccatctc tcatattttg
     4321 gaaagctatc cttctgagat ttccggtgga caaaaacaac gggtcgccgc cgcacgtgca
     4381 ttgatcacac aaccaacgat tctattaggg gatgagccta caggtgcgct ggattccaaa
     4441 agtgcccggg atcttttaga tacgatggac gaattgaata caaaagatca tgtatcgatt
     4501 cttcttgtga cgcatgatcc gttttctgca agttattgcc aacgtatttt attcatcaag
     4561 gatggcggta tccatcaaga agtgaaacgt ggagatcaaa gccgtgattc cttctatcga
     4621 gaaatactat ctattcttgg aaacttggaa cagtaggggg agattgacta tgctttggaa
     4681 attatcactt actgggatca agagccgttt aagagactac ttggtgttat tctcaggatt
     4741 agttatggca tctgctattt tttacatgtt tgaatcaatg gcaagcaacg aagctttttt
     4801 gaaaagtaat acgatcgttg cggctacagt tatcattttc cgattcggtt ctgttttact
     4861 agggatcatc acattcgttt atattttata tgcaaattcc tttttgatga cgatgcggca
     4921 aaaagactat gcaatgttta tgatgctggg agcaaaaggc agaaaaatcg cacagatgat
     4981 tttttctgaa acattcttag tagggatcgc agctacttta gtgggttcag ctgtcggtgt
     5041 aggattgaca agtatcgtcc accgattatt agtggatcag ttagatctca atatttcgca
     5101 ttttacacct tttagtattc acgggttgtt gatcactgtt ttgttttttg ctgttttgtt
     5161 tttattagct gctgttgtca atgctttttc gattgtcaaa aaaccgattt tagcattgat
     5221 tcgtgcggat cagacaccaa caagaatcaa gcagcataaa ttcttgtttt tccttgaagt
     5281 agtactggga attgcttttc taggctgcgg ttactatatg atgaaacacg tggggaccta
     5341 tcaagtcttt ggtatagggg tagccttagt tacgatcgta ttaggaactt atttcatttt
     5401 ccattctgtg atcattttct ttttatcatt actaaagaaa tcagaacaga tttcattgaa
     5461 aaaattgaac aatttcacat tgtcacagtt aaactttcgt attcgtgaat atacacaaat
     5521 gctctcaatg gtcgctatgc tgtttgcctt ggcactgggc gctttgacgg taggattagg
     5581 ttttaaaaat gaaattgcag acttgacaga aaacaattct ggatatgatc tagtgatgaa
     5641 taatcctcgg gcagaagatc agcaaaaaat caaagaactt tcaccaacac tttctgcttc
     5701 ttatactcaa aaagaagatg cgggaacgat ttactataat gcaagtgaat ttgcggctca
     5761 accattgatc atgataaaac atgaagatgg aacaccgccg aagatcacga aagaaaaagt
     5821 tcctgctaaa aaaatgaatg aactgactgt acaagaggaa cttcgaagct atgaattgcc
     5881 ggaacagcaa gaaaaagaaa tcaaggtagt cagtcaatca gaatttgatc aactgaactt
     5941 gccacaatcc actttacagc tagttcaggt aaaagacttc caagcaaacc tgaaaccaat
     6001 cgaaacactt gttgctcaaa ataaaatgaa caatccatct ttgcaaaaag tagagtacag
     6061 caaccagaaa tatgaaatgt atgaagtgta taatggtcta ttctctggtt ttgaatttat
     6121 gggattcttc ctaggtttag ctttcttgac catgcttgct agctgtttga tgttcaagat
     6181 cttgtcagga agcaagagtg acattgctcg ttatgaaatg cttgaaaaga taggaacacg
     6241 acgcgggttg cttaaacaat cgatccgtag agaagtaggc gtgttattct tagcaccagg
     6301 aatcttaggt gtagtccatg tcttattcgg attacaaatg tttggtctat tcatgcaaaa
     6361 tccatacaaa gatatttggg ttccgtttac gatattcttc gtattgtatt tcatctacta
     6421 tgtactgacg acatggttat atactggaat cgtattgaaa aagagattgt gaaaaagctg
     6481 tcctgcatca agtaataaga acagccaagc aaaaatagct cctcatattt ttcgtttggt
     6541 tgggcttatt accgcagatg caagcttttg aacaccgttt gttcggaaaa agaggttgtg
     6601 acaatatttt tgtcacaacc tttttttcac ggtcttttat tagaatatag taaatttttt
     6661 ctcgttactc acggaataag ctgttaagaa aacggtttcc atgtatactg gaatcagtgc
     6721 aaatgaaaga aaagagggtg caaggaacat ggaaatcaat gaacaaaatt ttggcgaaaa
     6781 ctatcacttg attacgatca cgaatcaaaa tgggacatct ttgtctgtca gtgatcttgg
     6841 ggcacgggtt gttcattttc aaacaataat cgataatgaa aaacgagagt tgatcttagg
     6901 ttttgatgaa gcaaaagaat atctagaaaa agacccttat atcggtgctt caatcggacg
     6961 tatggctgga cggattacga atggcacatt caaattagat gatgaaacgt tcgaagtaaa
     7021 aacagacccg gacactggtc attgtttaca tggtggtgca cctagctttg aagccaagca
     7081 gtggcattat acagtcatca atggtgaaaa cgaagcatcg gtcattttct atcagactag
     7141 cccagatatg gaaaacggat ttcctggaac tttagatgta gaagtacggt atacgctgac
     7201 gaacgatgat acatggcgag tgacggtcca agggatcagt gaccgcaaaa cagtattcaa
     7261 tcctacaaat catgtctatt tcaatttaac cggtgatcca gcacagtcaa tcgacgaaca
     7321 tatactttgg ttgaatagcc gattatttgc tacgctcaat aaagatacaa cgcctacagg
     7381 agaagtagtt tcagtaagtg gcacagcatt tgacttccaa cgtcctaaaa aattaggtga
     7441 ggtctttgcc agtgattttc gccaatcaat gatagtgggc ggcatcgatc atccattttt
     7501 gttgaaaaaa caatctggtt tactaaatgc tgcggaattg acaagcccag atgaaaaagt
     7561 atcgatccaa gtaaaaacag atgcttcagg aatcgtgatt tttacagcaa attttggaga
     7621 agatggaccg gaaatgcgcg ggaataaatt agtaaatcat ggcggtatca catttgagac
     7681 tcaagaactg ccaggagcgg agcgacttga aagttttgga gatgtcactc tggaacctaa
     7741 ccaagtacgc gagtttgtaa cggaatacaa aataaatata aagaaataga ataaattaaa
     7801 aagggagtgt gacagtgtca catccccttt ttaatttatt ctatttcttt aaaggcagat
     7861 caagccagaa tgatttgtct aaacttaaca aggttagtac aggaacagtt gcagctttaa
     7921 ctgtacgagt agttgtttct ggtatctgtc ttgccgtaaa attgttccac aaatcccaga
     7981 ttgaagcgac acggaagatg gaagaagcgt tgttgacgcg ttttatcgtt gaaaattcta
     8041 atcctgtgat tggcacgcca tctgttgtcc atttgattgg cacatcgaat cgcttagaca
     8101 tattgaggat ttcagcgttt gcttcttcag ttgttgctgc ttcttttttc ggttcatcat
     8161 attttgtcag gtcgtacgct tcgatgatcg cgttcaattt ttgtgaatat tggcgatctg
     8221 tagcataacg tccttccaaa tatttggttg cttctttgta gtcttttgtt tccgacttcc
     8281 aagttggttt gtagaaatca ggattgccag tcagtccatt tttgatcaag tcggcatagt
     8341 cttccaaact ttccttgtaa gaaggatatt tacggaattg tgcgcggatt tggtagcttt
     8401 gtccggaact atcttgttca agtgtattga aggtcacgct gcttccttta tatgatcctt
     8461 tgataccaaa aaggttatga tttggttcgc ttgataataa gctgtttcca gatccacttt
     8521 caaggatagc ttgagccatc atgactgatg cgtagattcc ttctttttgc ccgatttcct
     8581 gtgcatcatc tgcaatcttt ttcaagaact gatcagttgt ttggttttga ctgaaatgga
     8641 acgagccatc taatccatct aaaatagatg agccgcctgt cagcgaacct gaagcagcag
     8701 gtaaacctgc taagtttact cggacagcaa aatcaggcgt gaaatctgaa atagaagtta
     8761 ctcgaacttt atctcctggt tgaggcgcat ggataaattg tccgttccca atatagatac
     8821 ctacatggtg agtaacaccg gaagaaggtc cccagaagac gaggtcgcca ggttgggcct
     8881 gacttactgg aatctgcgta cctgcatatt gctgttcgcc tgtccagcca ccgatataat
     8941 gaccggttgc tttcatatat acgtaataag tcaaaccaga acagtcaaaa gtatttggac
     9001 cttttgctcc ccatacatat ggttttccaa gatgcttgaa tgcttctcga acgattgctt
     9061 gttggacatt cgttgtattt gtaggcaata cagcatcaat tggtgatttt acaacaaaat
     9121 cagaagaagc agaagatgga ttctctatac cgctttgatt tgctgtattt ggacgagatg
     9181 aagcattgtt cgttccgcca ttcgatgaac cattgtttgg atggtttggt tctctctgag
     9241 tatttccatt gttattcgat ccagatgggt ttgtttgatt cgaagaattc ccgctgttcg
     9301 aattattgtt attattgttt gaattatttg aaccattatt attgttgtta ttacccacat
     9361 ctggtttgtt tggcgtttcc ggatctggtg tactagggac ttcttcacta ggatcagttg
     9421 gtttctctgt tgaagtatcc ggtccagctt taaattctgc agtaatagat aaattgttcg
     9481 tacctactgt aaaggtcttt gcatcataat ttacttcttt ggcaccatta acagcgactg
     9541 aagcaatttg ttcaccttca gctggcgtaa tagtgtaagt taccttcgtt tccgctaata
     9601 aaccttctac cgttccactt tccgtaatat ccaaaggttg ctgttcaggg gaatccgctg
     9661 tcgaagcagt cagttggatc ttaccaatca aagaaggagc aattgtaagg gaatatgtct
     9721 cggctggctt ttcattttct gatggatcag ccggatcaat ttcagttggc tcggtcgatc
     9781 ctgttgttga gttagttgtt ccagttgggt cttctgtaaa agaatccgtt gtttcactag
     9841 tagctgagct ggatgtagtc gtatctgttg tgactgtttg atcagctatt tgattatcta
     9901 cggcaaatac ggtattggtc gacaaaacag acggagcaat cagccaagta gcagctagca
     9961 aacgtatcgt ttttttattt ttcataataa actagtcctt ttcctttcat tgattttctg
    10021 tcttattcag cattcattag acattttttg gaacagataa tcgtgtcgaa aagctttttc
    10081 ttttcataaa tctgtctgtg tattctaaat aaaacaattt gtaaataagc aacgagcatt
    10141 cattcgtttt tctttgacgt tcattttatt gcatagacca cctttgtttg gggataaaca
    10201 agtggggata aaaatggtta tgcatatgta aattatacca aagtttggaa agtatcacaa
    10261 aaatttataa aaataatgat cctaaaaaga aaaagactga aatatgtgat aaacatctca
    10321 tacttcagtc tttttcatta actattcata agacattagg tcttcatcaa acccgctaac
    10381 gagagtagtg atcattgcta cgactagact aacagccagt cctgctaagt accagaaaaa
    10441 agtaccggaa atgaatgcag gcaatgtggt cagactacca aatacaaaag cgatcgcttt
    10501 cacttgggca aatcccatta cagctccacc agcagctgca ccaatgcaag cagcaacaaa
    10561 tggttttttc aatgggatcg caattccgta aacagttggt tcggtcactc ccgcaagtaa
    10621 aatcgaagca aaagcagaac cggagatcga acgggttttt gaacgtttac ttcgcagaaa
    10681 tacacctaaa gctgcaccag ccattccaaa attggctgct cctgtcaacg gaacgattcg
    10741 gtcgaaccca taagcagaga tattactgat catgacaggg tttacagccc attggatacc
    10801 aaatattacc aaaatattcc aaccaccagc gatgattgcg ccactgatcg gtccgttaat
    10861 atccatcatc caagtaattg cggcagctaa accttcgcca aggtaaacac ccgcaggccc
    10921 gaataccatt aacgtcaaag gaaccgtgat aactaatgaa agtaaaggta caaatactaa
    10981 ttgcatattt tcgcgaatat attttttgag gaatttttca acaaaagaat aaaagaaaat
    11041 acctaaaatc gccggcatga cagtagacgt atactgcatc agtactacag gaatccccag
    11101 aaaatcagta gctgctttgc cactgtctga aagtaaagag gtcagattcg gttcaatcag
    11161 tgctgcagca atgacagccg caatatatcc attcacttta aacgttttgg cagcagaaaa
    11221 agcaagaaag attggcagaa aataaaagac ggcattcgac gcagcagaca aaatatgata
    11281 agttccgctt gtttcactaa gccagccaat cgtagtcgct aaaacaacca gaccttttaa
    11341 tataccagaa ccggcaaaca gcggaatgat cggcgtaaag atttccgata tcaaaccaaa
    11401 tagtttttcg atcggattta gcttttcttt cggagctgaa tgatcgattt gacgcgtcaa
    11461 ttccttgtaa gcaggagcca atccctgatc catgattatt tctgcatatc tatcgagtaa
    11521 aatgaaatac ttaatggaag ggatttgctg ttttaatttt tctgcattgg ctactggctg
    11581 ttttttcaat ttgatacgaa gccgagtcgc acatggaagg acagaatcaa tattgtcttt
    11641 tcctcccaac tgttcaatca gcatatgaat ctgctcggac agtaccatct gttcaggatt
    11701 cattgtcgtt tccattagaa gaccactcca ctcactaata taatattggc gtaaggtgtc
    11761 atttcgcctg tacgaataaa tgcatgacag ttattcaact cttttttcat ttcttcatgt
    11821 gggataaagc taatcggtgt atctggtaac aataatttga ttttttccaa tatttccggg
    11881 ttcattgtct ttatttcttc cgccaattca atcgattgta cctcaagttc ttctaaaacg
    11941 ttttctaaaa cagataaaaa acttggaaga ccgtttgaga cagccaaatc tattttttca
    12001 gtaccaaagg ggacaggcat gcctgcatcc ccgatactta atttatcaaa gtgccccatt
    12061 tgtgcgatta ctcgtgaaat atctgaattg attacttttc cttttttcat gtttgtttct
    12121 tcctctcatt gttaatttta gactgctgat tgttctaaag cttcgcggta agggatagaa
    12181 ggttgcgcac cataagactg cacagcaaca gaagaggcta gatttccata aaggatcgct
    12241 tctttcaagt tactaaggtc tggctgaagt gtgctgctta atgcgccaat aaatgtatca
    12301 cctgcggctg ttgtgtcaag ggcatccact ttaaaagcag ggacgatacc gtgttctttt
    12361 tctgtatgat agaaagcgcc ggcactgcct agtgtaatga ttactgttcc aatacctaat
    12421 tcatggagtt tttcggctgc tgcgaccaaa ctgttatgat ctgtcactct gattcctgta
    12481 atgatttctg tttctgtttc atttggtata atgatatctg tcttttcaag tagttcagta
    12541 ggaatcgttt cacgagctgg tgcaggattc aggatcgttg tttttcctgc gtcttttgca
    12601 atagaaaaag ctaggatcgt cgcatctaag ctactttcaa actgagcaat aataaagtca
    12661 ctgtttgtaa tcagttgctt gttttttagg acttcttgtt catggaaagc attatttgcc
    12721 cctgcatgga tcaagatact attttctcct gctgcgtcta ccataatcat tgcttgtccg
    12781 gttgtggcgc cttggatttc agctatccca gatgtatcga tgttttcttt tgtcagtaga
    12841 tctaataact gttgtccttc gctgtcagcc ccaacgcctc caataaaaga tgtccgtgcg
    12901 cctgaacgtt tggctgccac tgcttggttg gcacctttac ctccgccagc gtgaaaaatc
    12961 tcatgcgcat gcatcgtttc gcctggcttg ggcatgttgg ttaaacgaag ggtagtgtcc
    13021 atattaatac ttccaattac tgtaaccgtg ttcattttac aaacctccaa ctatctcatt
    13081 tattttgatg ggaaaattat tttttaaaac gttttagtaa aacgttttaa tagttcgata
    13141 aaaatatagc acaccttgaa agctatttca agggtgcagt tgaaaatctt ttttctaatc
    13201 gtacaggtaa tcttttttct tcccattctt tttctgggaa ttggatccga tctaacaata
    13261 acttggcaga tgtttgtccc aattcgaaga ttggttgtgc gatcgttgtc agtttcggct
    13321 tgatatactc gcacatatct atattatcat agccaataat gctgtagtct tcagggatgc
    13381 tttttccagc ttcctctaat ccgcgatata atccaaaagc taattcatca tttaaagcaa
    13441 acacaccagt tgcagcagaa tcaagaagtt ctgctgtgat ctggtagcca ccttgttttg
    13501 agaactgtgt aggtaagagg atcagttggt cgtgagggat ttggtaaaca tcaagagcag
    13561 atttaaaccc ctcaatccga gcgtggacat tttccggtgg attttcagga tagacaaggg
    13621 cgatcgtttg atgtcctaga ctaagcaagt gcatgcccgc taggtaacca ccgcgaaaat
    13681 cgtctgttcg tacggcatca ctaaatcctt cagatttttt ctgatccaaa acaatgaagg
    13741 gccggccttg ttttttcaaa ttttcgttga ttgcatctgt agagacagca gaagtcgcga
    13801 taatgaatcc atccacacct cgacgtgtga gctcggctag atattcgatt tctttttgat
    13861 gatcagaatc agcattacat aatatcgtga cgaaattctg cttatacaag atatcctcaa
    13921 ttcctcgcat cagtgtactg aagaatggat tcgtgatatc aggaacgagt acaccaattg
    13981 ttttcgtttc cttggtaatc atttgtctag caaaatagtc aggctgatag cctaattctt
    14041 ctttggcagc taaaactttc tgaacagttt tcggactgaa tttctccgta tttccattta
    14101 atatcaagga gacagttgtg attgaaacgc cggaaagttt agcgacttct ttgattgtga
    14161 tctttttttt caccatgttc atactcctat ctaaatcctg tggttatttt cccatgaaat
    14221 caaaaagaag taaatgaaaa tcacgtgtgc cagtacttat ttgctttgtt ggatagcttc
    14281 gattatttgt ttcgttgagc gtactcgtgc agttttcgga aatatctttt ttatgaacaa
    14341 ttgatgggat tctttgtcac gatcactgca agcatcttca gcaagcacga tatggtaccc
    14401 gtgttgaaag gcatctaatg cagtagcata tacaccgttt gttgtcgcta caccgcttaa
    14461 aatgatagtt tcgattccac gccggcgaag ttgaagatca agagaagtac caaaaaaagc
    14521 acttggattg tattttgtga tagtcaacat attatctgta tctttcaaag aatccttcaa
    14581 aagtaaatca gtgaattctg gaggtacaga agcagtcatt ccacccatat cagtttgttg
    14641 actcaaataa gggaaagtgg agatgtctac gttcactaaa actttcaagg cagcggtacg
    14701 tttcaatgtt cttactaaca aatcattgtt tgtcagaata gatgctgcag tgtgtggaga
    14761 gagtgtaccg ttatttacga tctctttttg catgtcgatc gcgacaaaag cagtttttga
    14821 taaatcaaaa atcatatttt cctttaaatc attcatgatg ttccttcttt caaataacaa
    14881 tgttttttat gtaagtaaat cataaatcgt ttatttgtca actaaattaa aggattcctt
    14941 tctttagtct agagaaattc agtggtaaaa taaataagtg tgagaaaacg cattcgatgc
    15001 atagatatag cgagaaatat cgtgaaaatg gaggaatcag agtgaagtat gtcgaagtaa
    15061 aaaatgaata caaacgatac aaaatgggag aaacgaccat tacagcaaat gacgatattt
    15121 ctttttcaat tgaaaaaggc gaattagtga ttatacttgg tccaagtgga gcaggaaagt
    15181 caacgattct taatatttta ggtgggatgg atacacctga tgaaggtcag atcattattg
    15241 atgacacaga tattgcgcaa ttttcagata aacagcttac tgcttaccga cgaacagatg
    15301 tagggttcgt tttccaattc tacaatctcg ttcctaatct aacagcaaaa gaaaatgtgg
    15361 aattggcaac ggaagtttct cctgatgcgc ttgatcctgt agaggtattg aggcaagtgg
    15421 gcctagctca tcgattgaat aactttccgt cccaattatc aggaggcgaa cagcagaggg
    15481 tttcaattgc tcgtgcacta gctaaaaatc ccaaacttct tttatgtgat gaaccgacag
    15541 gcgcgctgga ttttgaaacg ggcaagcaag tattgaaatt attgcaaaac gctagcagac
    15601 aacacggcaa taccgttttg atcattacac ataattctgc tttagcgccc atcgctgata
    15661 gagtcattca cattaatgat gctaaagtaa ggtctgttga attaaatgat catccttcat
    15721 ccattgatga gattgtatgg tagggggatt ttatgaaaac aagaacatat cttaaagcag
    15781 gttttcgcga aattcggcaa tctaaaggac gattcattgc aatcatcctt attgtcatgc
    15841 taggaacact tttatttgta ggtgtcaaaa ctgctggacc tgtcatgcaa aaaacgatgg
    15901 atcactatgt tggtacagca ggactttctg atttgcaaat cgtttctact ggtggcttga
    15961 cacaaaaaga tattgctcaa gcagaaaaga tcccagatgc aaaagtagaa acaggaaaac
    16021 agatctatta ttccaatact ggcaaaaacg aagtgattca aatattttct tataacaaaa
    16081 ataacaagca gaacaagcta caattaactg atggaaaact cccggaaaaa ccaaaccagc
    16141 ttgtattgga tgaaaaagcg gaagatgaag ggtataaaat cggtgatatt tatcagattg
    16201 attcagatga attgaaagaa aaagagtata ccatcacggg ctttgtccgt tctccattgt
    16261 tcatcaacaa tttggagaga ggatacgcga acgtcggaaa tggtagcgtc gattattttg
    16321 tctatttacc tgaatccgtt tttaaaacag atatttattc tgtcttatat cttgatttta
    16381 caaacgtaaa gaatttgaat acgtacagca aagcttacaa agataaaatg gagcaaaatc
    16441 aagaaaaggc agaaaagtat ttacaagatc gacccgaaga acgcttagaa gagctgaaaa
    16501 aatcggccaa cgaagaatta gaaccggcac agcaaaaaat agcggatggc aaagcacaaa
    16561 caacgcaagc aagaacacgg ttagaagatg ctaaaaaaca gttagcgcaa cagtcagcaa
    16621 ctatccagca gcttcctgaa gcgcaacgaa caatcgctgt tcaaacatta tctcaacaag
    16681 aagctcaact tgctgaacaa gaaaggcaat tagttgagaa agaacgtgaa ctggcctctg
    16741 cagaggaaga attgaatcga aatgcagaaa aaattgaaaa tttaactaaa ccatcatacc
    16801 tatacaatga acgttcagaa aatgtcggtt ttcaagaatt tggtgatctg tctgatcgta
    16861 tcgctgcgat tgccaatgtt tttcctgttt tctttttctt tattgccgca ttgatcacct
    16921 ttacgacaat gacacgtatg gtagaagaaa accgcaggga aatcgggaca ttaaaggcgt
    16981 taggttatac gaaagttgaa atcgcaaaaa aatatgcaat ctatgcaagt ctagcctcag
    17041 ggataggtat tgttttagga acgatattag gaacgaatct tttgccaagg atcatttttg
    17101 agctgtctaa cgagcggtat gatattggga gtgcgcttat tttttatgac tggccgccga
    17161 ttttacaagc agctgccgca ttcttcattg ccgcatttgg cgctgcaatg atcgttttat
    17221 tcaaagattt aagagaacgg cctgctgcat tgcttcaacc gaaagcaccg aagccaggga
    17281 aacgcatttt tcttgaatac atcactccgc tgtggtcacg ccttagcttt aatcaaaaaa
    17341 tcagttaccg aaatctcttc cgatataagt caagaatgct tatggcgatt atcggaattg
    17401 ctggatgtgc cgggctgatg gttgcaggcg tgggattgaa agattctcta agttctgttt
    17461 cagccaaaca atttggaccg atcattgact atcaagcaat cgttacattg gattcatcca
    17521 cggaagatag tcgtcaaaaa gcagcggata tgctgcagaa gcaagaaaaa gtaaatgaga
    17581 tcctagctat tcatactcaa accgtagaat tgagaaaaaa aggacatgct tcgcaaagtc
    17641 tgacagtgat cgtaccaagt aaaacagctg atttggatcg ttttatccat ttaactgatc
    17701 ttgaagagaa cccacgctcc ttgcctaaaa caggaatcgc tattacgcaa aaagtagcag
    17761 aactatttga cttatctaca ggagatcata tttcgcttta tgatgaagat caaaaagaat
    17821 ggaaagtcga agtaaaagaa atcgtgaaaa actatctagg tagctttatt tatatgtccc
    17881 ctgaatatta tgcttcagtt actgatcaat cgatgatgga taatgcattt ttgctgcgta
    17941 cagataagat gacaacaaaa gaagaagaaa tgctttccga gaaactgctt gccacagacg
    18001 ctgtgacaaa tacgtctttc atgtccaaac aaatcgaaac gcaagaagaa tcaatggcga
    18061 acttagactc ggttgtctta atctttgtcg tattatcagg actgctggct tttatcgtct
    18121 tgtataatct aactaatatc aatatttccg agcgtgtacg cgaattatct acaataaaag
    18181 tccttggatt ttttgataaa gaagtcacga tgtacattgt tcgagaaaat atcatcttta
    18241 ctcttttagg aatcatcgga ggatttggtg taggatatat attgactgac ttcatcttgc
    18301 aacaagcgtc tatggagaat gtcatctttc cattagtgat cacatggtgg gcttatgtct
    18361 tatcagcagg attaactatc gtatttacgg ttattgtgat gattgttacc catttcaaat
    18421 taaagcatat cgatatgatt gatgcgttga aatcgaatga ataaaaacag aggttgtgaa
    18481 atcagcggtt tgacctgagt attagagaaa caaacggcaa aatagttttt catattttgc
    18541 cgttttttga gctaataccg aaggacagct gattgaacac catgaatcag aggttgtaaa
    18601 aaagccgtcc tgcatcgagc aaaaagaata ctcaaataaa attagctcct catgttttac
    18661 atttgagaag actgattgcc gtagataaaa gcttttgaac accatttata gaagaaacaa
    18721 gaatattttg ctattttaaa caagaggata gacaaataca tgtctgatct cttgtttttt
    18781 attgggcaac agacctctta gaatgtataa agataaattt tacaaacgct ttcggttcag
    18841 ttataataat aaatgataac ttgtatatat aagttgtggt aggtaataaa tcagttgaat
    18901 ggaggtaaat atccatgaca tttaaagaca aagtcattta ccaaatctat cccaaatctt
    18961 acaaagacag taatggtgat ggaattggag atttaaaagg aatcaaagaa aaactcccgt
    19021 atctgaatga tctaggcatc gacatgattt ggctaaaccc tatttatcca agccctcaaa
    19081 aagacaacgg ttatgatatc tctgattaca cagccatcga tcctatcttt ggtacgatgg
    19141 aggaattcga acagctaata gccgaagcaa agaaataccg gatagaaatc atgctcgata
    19201 tggtgctgaa tcacgtttcg acagaacatg aatggttcaa aaaagcacta gcaggggacc
    19261 agcattatca ggacttcttc attttaagag aagagccaac ggattgggta tcaaaatttg
    19321 gtggaagtgc ttggtcgcca tttggagata cggggaaata ttatttgcac ttatatgatc
    19381 ggacacaagc tgacttgaac tggcggaacc aagcagtaag agaggaacta ttcaaagttg
    19441 ttcgtttttg gatggaaaaa ggggtaaaag gattccgttt cgatgttatc aatgttatcg
    19501 gtaaagacga acaattgaaa aataataaag aaaatgatgg caaacctgaa tatacggaca
    19561 aaccaatcac ccatacgtat ctaaaagaat tgaaccaagc aacgtttggg acggattcca
    19621 ctatcatcac agtcggtgaa atgagctcta cgacgatcga aaattgtatc ttgtatacag
    19681 aacctagtag agaagaatta tcaatggtct ttaatttcca tcatttgaaa gtcgattatg
    19741 aaaatgggaa taaatggtct actcctccat ttgattttgc agaattaaag cgactatttc
    19801 atacatgggg agaaaagatg agtgagggta acggctggaa tgctctattt tttaataatc
    19861 atgatcagcc acgtgcgctc aatcgatttg tagctatcga tcagtatcga aaacaaggag
    19921 cagagatgct agcaaccatg atccatttga accgtgggac gccctatatt tatatgggag
    19981 aagaaatcgg catgatcgac ccagactatt catcgatttc cgactatatt gacgtagaaa
    20041 gtttgaatgc ttataagtta ctccttgaac aaggattcac agaaaaagaa gcgtttcgtc
    20101 ggatcaaagc aaaatctcgc gataactctc gaacacccat gcaatggtca gacacagaac
    20161 aagcaggatt tactacaggg aaaccttggt taaaagttgg aggcaagcta gaagagatta
    20221 atgtagaaaa agaactatct tctgaagatt ctattttgtc ctattacaag aaattgattc
    20281 ggctgagaaa aacatatcca atcgttgcac aaggagatta tcatgcttat gaagcaaatc
    20341 atccggaagt ttatggcttt cttcgccaat ttgaaggaca gcagctgctt gtattaacaa
    20401 atttctatga taaagataca aggatcgcca tcccagaagg attcacagaa gccaatattc
    20461 ttatcgataa ttattccgat actgttcttc atcaaagtct ggagctaaaa ccataccagg
    20521 caattgcttt gctgaaaaat taaaacaaaa acacaaaacg tcgttcctac gagctattct
    20581 cgtaagatcg acgttttgtg ttttatttaa aataatcttc ttcagaaaat gaagagtttt
    20641 catactctcc tttatcgcca taaaatttat tgtagcgttg tttgaattta cggaaaataa
    20701 aagtaacgag taatttgact aacgtataga taggaatacc aaaaataacg cccacaattc
    20761 ccattagatc acccatgaca agtaaaacga ttaagatcgt aatcggatgg atctgtaaac
    20821 gatcacccat tactcgagga atcaccaagt caccgtggag aagttgtacg gcaaaccaga
    20881 caatgacaaa tttgacagcc ataaaagtag agtcttggaa tgcaatgatc aaacctggaa
    20941 agaaggcgat aaacggccca atatatggaa tgatacataa caacccagca gaaatagcta
    21001 aaacactagc aaattctaat ccgatcagta agaaacaaac ccagtagact gctccaagga
    21061 taaatgaagc aatgatttgc ccctttagaa atgaacccaa ttgttgatta gcaatctttg
    21121 tcaaattttt aaaatctgta cgaaatgctg gtgggacaat cgctaaaact gactgataaa
    21181 atttttgagg atctctcaaa aggaaaaaag cgacaattgg accagtaaat aaagtaataa
    21241 aaatagctgt aacagttgaa aaaatgttgc tgatccgttg tgcgccactt tcccaataat
    21301 ttccaataaa tccagctata tcagacgtca cgtcgtcaat agaagcgaaa aattgattaa
    21361 atgaatcggc aaaaggtgtt ttctccaaaa attgtcggag actctcttga aaatcttgaa
    21421 acaaatctgg caaactactg atcaatgtct ttgcctgttc ttcaagaaaa ggaataagcc
    21481 atatgcttcc aagccccaat aaaaataaaa taatcacata aacgataatg actgacaagt
    21541 tcctcgaaag gtgcttgctc aaacgtttga tgactggttc caaaagataa tacaagatca
    21601 gtgcaaacaa taatgggggc aagatagtag cgataatgat tccgacaggc ttgaatataa
    21661 acgagatctg atggaaaaaa taaatgatca gcgcaagtaa gataactatt cctaaaacat
    21721 agtaagaggt ttttcctcct aaaaagcgga tcaaacgatt cgttgtatct gtttcttttt
    21781 tcactctttt tcctccttta tctataactt cattatagac aaaagcgggt ataaaaggaa
    21841 aatgatagag gttgattgca aaaatatcgt caatcattca tataaacttt ctcatcaatt
    21901 aaaataagaa aacgcttgac agttctttta accatatgat aaattaaatc ctgttgctaa
    21961 aacgtaacta ttacggtttg cgaaaagcga atcggaacat tcaaatgaaa gaagatgaga
    22021 agaggagtgt cataaatgaa acgattgata ggtatattgg ccatgctggt tttagcaggt
    22081 atgttaactg cttgcggggc aagtgggaaa gccgaggaca gtaaagaaaa attgagcgtg
    22141 atgacgacct tttatcccat gtatgatttt acaaaagcaa tcgtcgggga tgaaggagaa
    22201 gtagagttat tgattccagc agggactgat tcccacgatt atgaaccttc tgctaaagac
    22261 atggcaaaga tccaagatac agatatcttt gtttataatg atgaaaacat ggaaacatgg
    22321 gtccccgcaa tccaaaaaac attacaagaa ggcaatgtcc atacgataaa agcgacagaa
    22381 ggtatgcttt tacttccggg aagtgaagaa ggacatgatc atgaccatga acacggggaa
    22441 gaaggccata cacatgagct agacccacat gtatggctag ctccaagttt agcaatcaag
    22501 caggtagcaa acatacgtga tcagctgatt gaagcttacc cagaaaaaca ggaagtatgg
    22561 acaaaaaatg cagcagccta taccgagaaa cttcaagcac tccatcagct ttatcaagaa
    22621 acctttaagc aagcaaaaca aagatctttt gtcacgcaac atgctgcatt caactatcta
    22681 gcgttagaat atggattgaa tcaagtatcg attgctggtc tttcttctag tgaagaaccg
    22741 tctgcggcga gaattgctga actgaaacac tttgttaaag aacatggaat caactatatt
    22801 tattttgaag aaaacgccaa agatagtatt gccagaacgt tagcaaacga agcaggtgta
    22861 tctttagaag tattgaatcc tttggaagga ttgacgaatg aacaaataga aaatggagaa
    22921 aactatcttt ctatcatgga agcaaacctc gaagcgttga aaaaaacgac agagacagaa
    22981 aacccgctag aagatacttt gacccccaaa aaagaaaaga gcgtctacaa cggttatttt
    23041 gaagacgagg atgtacaaga tcgctctctt tcagattggg caggaacttg gcaaacagta
    23101 gatacgtatg tagaagatgg gacttttgat cctgtttttg aatataaaga aaaattgaat
    23161 caggataaga cagcacaaga atataaagaa tattatacga agggctatca aacagatatt
    23221 gcttcaatca agatcagtga gaaagagatg gcatttacgt ttaagaatgg aacaacgaaa
    23281 cgttcaaatt atcgttatgt agggaaagaa atcttgacct acgaggcagg aaaccgtggc
    23341 gtaaggtatc tattcgaagc agaagataag gaaagtggcg cttttagata tattcaattc
    23401 agtgatcaca cgattgcacc ggctgactcc gaacattttc atttatatgc aggaaatgaa
    23461 agtcaagaag tattgctgaa agaaatggag aattggccaa cattttatcc agatacaatg
    23521 gatggacgtg aaattgcaca ggaaatgctt ttgcactgat aggcaaaaaa gaaccgaagg
    23581 gtttggatcg gctggtcatt tagtcggtcc aaactattcg gttcttttta tgaaatcaat
    23641 ttctttaatc cacggataga ggctgggatg gtctcgccgt ttgccaacgt gacttctctg
    23701 gtttttttat taatcgaccg cacattttgt gtattgataa cataggattt atgacaacgg
    23761 ataaaattcc gatgcatttt ctcgatttct ttgatcttct catagaattg taattgggca
    23821 tgagtcaaat ggacttccag tttatgtggc gctcctagcg aagtagaaaa atattggatg
    23881 tctttgatcg gtaaaaattt gatctcatca ttaaactcta ttttgaagat atctttttgg
    23941 atttgggaca tttgaaaatc ttcgaaaatt ttgctcagga tatgctcgac attttctttt
    24001 aaatcataaa gatcttcttt gacgatataa tcagtcggtt cgatgttgct ttttaagatt
    24061 ttcatcgaca tttccgtatg ggaagtcaca tagattattt ttcctaaagg atctaattca
    24121 cggatcaatt taccgatcgc tacaccatcc atttcactgt cttctagttc gatatctaaa
    24181 aagtaaatac caaaagaaga agttctgatc ttggctgcat tgagaagttc ttccggatca
    24241 gaggtagaaa tatggacata actgtctaag ttttcaatca tgattctatt tttgatcacc
    24301 gttgataaaa cttctaattg ttttggatcg tcttcacaaa tataaatggg aatcatatca
    24361 ttactccttt attttatttg tatataaatc gtttgagaaa aacgctggtt ttcaattgat
    24421 gtttcaagga aaagataagg attggtcttt gtcaattcat ccaagataaa caagccaagg
    24481 ccttcgtttt ttggatttgt ttttgttgag aacccttttt gtttcaactg gtttaacggt
    24541 aatgtatttg ttgttgtatt gatcacacga atcatcacgg aatgattctt cttgagtatg
    24601 gtcaattcga tttttttctt ctttgcagca ctagctcctt caatcgcatt gtccaagata
    24661 ataccaagca tacgtacaag gtttaccgta tgtttcgtat ccagttcgat tggctcagga
    24721 aaatgaagct ggacgatcaa ccctttttcc tgtgccatca tgagttttaa tgacagtaaa
    24781 ctacggatct caggaacttt catattatcc aacatactta gattggccgg aaaaaggttc
    24841 atcatctggc gtgtaggtaa tatcgtttga tcataataag accgtaatcc ttctaagtcg
    24901 ccttctgaaa tatagctgtg aagagaaaaa agcagatttt ggtaatcatg tttgaattct
    24961 ctcaagtctt gatagttttt ttctagatct ttgatataat cctgttgcat cctgacttgt
    25021 tgctctaata actgatcagc taatttctta ttagagattt tccaataata aaccaagatg
    25081 atcaggccaa cacacagata tagaatgaaa aaactcgtat tcaaagctaa gacatcaggt
    25141 gtttcaccag aaaaacgggt ataaaaaata atgatatagt atgtaaataa tatcaatgtc
    25201 ccgatcgtac cgatcatacg gcggtccaca gtgtgaaacc aagtctctaa cgattttttt
    25261 aaacaaaaag ctaataaagc agataaaaaa gcggataaga caatgtgaaa atactgcaat
    25321 gaatctcctg gttcaataac cgaagatttc aaaacaaaaa aatcgccgat gcttgttaag
    25381 tgatctccta aaagcgacgt caataaccca aaaggaagcg cgtaaaccaa ttgttgacgc
    25441 gaagtcgaaa agaagaaaaa agtaagcatc agcattccta tagcaattag agcagtaaaa
    25501 atccctaaaa tcgaatagac ccatccaatc aaaatacaag aaatcagaat gactcccgca
    25561 aacagatatt tgtatcggat gtgatggtca atcaataaga tacaaattgt gtaaatcaaa
    25621 tattgcaacg ctatggacca taaaaacata aagtacagct cccattgatt tttaccaaat
    25681 tatagcacaa ttggcgtatt atttttttaa tttttcaggg attttgaatt cagaactcat
    25741 accaaagcag cagccagata caaaaaagtc agataagtta gttaattgtt taatgattgt
    25801 ttttttcatg aaagattcct ccaaaattca tcaagtagtt tgtaatagga agcaacgtag
    25861 ctatttctaa aacaatccca agtagcaaat aggtagaaaa gtaagaagct gagaaaaata
    25921 acgtaatgag agaaaaaatt agaaacatgc tgatcgcctt ctttaataat gggggacgtt
    25981 tctcgggttc aatgggattg attgctgtac ctcttgatcc atagcgaagc agtaacaaaa
    26041 agcagctgat taacaaaaag accatcattg ggaaggagat agtcaattgc agatgtttca
    26101 tcaccaaggg aacgccccat aaataaagta gtgtccacag cagacaaacg aattcagagt
    26161 cggaatgcaa tccataagca tatctgcgga tgcagatata agaaagaaag gtgattcctg
    26221 tttccgccca tgagcctgtc accagagaaa agactagaac gaccgctagt tttccaagat
    26281 tattcaataa aacttctaag acaaatttta cttggacata caccatatct tcttccgatt
    26341 tgtctccgct gaataaggct tctaataatt tgttcacaac tttatctttc atctcatacg
    26401 tacctccacc acaaatttta tgctaaaaaa aataaaaaac gtcttttgtt tcataaaaag
    26461 tggttttgac atcaaaaccg tcatgatctc ctaaaaaaat cttcgttttc caagaacctt
    26521 accaaaaaaa gtgacagttt tgatacgaaa agtacttttt atgacaagtt tgtgcttttt
    26581 cgagtgattt atgttaaaaa attactgacc ttataaaagg tgatggaggg attcttatgg
    26641 aaaatgaaca attgtttgaa gtcggcgaaa catatgcatt taaatctgat ttatttgaat
    26701 caccaataaa aggaaaaata gaaaaaatct atgaaaatag tgtgatgata cgtgtcatga
    26761 aatgcgcagt agaagacaat gataccgctc ataatctgca ctggcagtta gttgtacgca
    26821 aatcaagtac aatagaagaa taaaaacaga taaaatgttg agagcataaa gaggttgtga
    26881 gtctcccgtt tagctctgag ttacaaggaa taatgttaaa aaacagcttt tcatgtttta
    26941 tgaatattgc cttgtgaccg aggtgttggc aggagaatac tgtttatttg aaaaaagggc
    27001 tgcgacaaga cttttgtcac agcctttatt ttcatgaata ggagttactt ttggctactt
    27061 ttagagaagt agcttttaag gagcagataa agtcaaggct tttatttcta taaatgaagt
    27121 agtaaaatga agaacagatt ttattacagg aaggatgccg ataattgtga tcattgttgc
    27181 aggaacgatc ggtgcaggga aaagcacact aactgaaatg cttgcccaag atttagaaac
    27241 aaaacctttt tatgagaacg tggaagataa tgaagtattg ccgctatttt attctaatcc
    27301 agaaaagtat acttttttat tacagatttt ctttttgaac aaacgctttt tggcaatcaa
    27361 agatgctttc agccatgatg acaacgtact agaccgctcc atctacgaag atagtatgct
    27421 gtttcatttg aatgcagatt tagggcgtgt ttctgaagta gaagtaaagc agtatgaagg
    27481 tcttttagaa acgatgctga aggagttaga agaaatttcg ccacaaaaga aaccagatct
    27541 actggtttat attcgtgtat cctttgaaac tatgttagcg agaatcaaaa aaagaggccg
    27601 ggaatatgaa cagctagaac aagatcctga actctattcc tactataaag aactcaatcg
    27661 acgatacgaa gagtggtatg aacagtttga tatatgtccc aaaatagtga tcgatgggga
    27721 caaatatgat ttcgtagccg atccggcatg tggagaacaa atcgtacaag aaatcaagat
    27781 gcgggcaaag aaaatgacag aagaggcaga tgcccagtcg atccgctaca ctacaaataa
    27841 cccgaaagca aagggaatct ttacaactaa ataatataaa aactaagcaa attgctcact
    27901 cgcagatatt tctctgtgag cagcatcttg cttagttttt ttgattcatt gagatcaaat
    27961 agtgaaccga ttcgtatgat aaaattgcca taaatgaaat gtctgagtga gggccatgat
    28021 caaaaataaa atggaaacga tcaaaatcga atgggcgcca aacagatgat gacagaaaac
    28081 aaggacaata gctcctgcga taaaggcaag aacgacactt agatagtgga aaaatcgatc
    28141 aaaatctgct ttttctttag aaatacaata atcatataag ttcgttgcta ccccttttaa
    28201 atttccagtt gtaaataaat tagtgtaggc acgcccgttg attttatcaa aagctaccca
    28261 ctggatcgat gcagtaaaag caatcaaaat cgttactaat gcgggatgct catggacggg
    28321 tgtcaaacta acaaagaaaa aaatcactgc ttcataatat agaacaaaca agcgccagaa
    28381 atgtttttct ttttttctaa aataatcaat tagatacttt gttagaagaa tccctaagaa
    28441 aaagaaaagg gtagacaata cttttttacc aacacttagc cattctccgt caaatgcttg
    28501 gatgatcgat agaatgatat tgccagtttg agcagaggca aaagcaccat attggatata
    28561 agtataactg tccatggctc ctccgataaa tgtcagcaaa agtcctattg tacgcccctc
    28621 gtgaaaggaa gtctgcattt ttttcatcac cttttatgaa tatttttgtc ttccttatct
    28681 tagcataaac gattgaaaaa aaggaaaaat tttcagaaaa ctaaaatcat atcgcttccc
    28741 attttcattt ttagaaaggt ctgttagtat aaagaaaaaa tagacaacga aactttaaat
    28801 aaccaacttg aaataaacaa attcaaagaa gaaagatggg aaggaaatca atcagttgaa
    28861 tgaaaaagaa attctccgat tacaaaaaga attgaaacgc tgggagaaca agtataaaaa
    28921 gcttcaacaa gtctttcggc tacaagaaga aatcattgct acctatgaag cggcttatgg
    28981 cgaagcaccc ggacttattg aggaagaagt acaagtaaaa gaaagtgaag gaaacagctc
    29041 tttctttaaa atgatggaga aacgttccta taatcgcttc aatcaagaac aaaaagcagc
    29101 tatcacatat gatatgaaga aacatcttcg aatcatagca ggagctggga gcggcaaaac
    29161 gcaaacaatc tgtgcaaaag ctgtttatct gatgacgcaa aaacaagtag acgaagaacg
    29221 catactaatg atcactttta caaggaacgc tgcaaatgaa ctaaaaaaac gagtagataa
    29281 tttcagccag agaaaaaccg ctgtccatat cggaacattc catagtatct tttttaggat
    29341 atacaatgaa atttgtcgaa aatttcctga agtagcaatg caaggaatac aaggagactt
    29401 ttcaaaggat actgcacaaa aagtgaatgc tgttttgcaa cagctgatac gtaaatataa
    29461 tctttatata tttgatcaat atggagaaaa aacgattgct tctcggttag actattggca
    29521 gaatatgaac ctttcagtag aagaaatggt tcagttagtt aaagaaaaat atgattccat
    29581 tgataagaat gcacgacagc cgatatcaga acgactgtat ggattgctca cggaactcca
    29641 agaacaaaaa agaagccagc aattgctaga gttcaatgat gtactccaaa atctgaaaag
    29701 cgctttggag aatgaggaga tccagcatta tatcggccaa aaatatgatt atctattcat
    29761 tgatgaattt caagatacta atccacttca atgggaaatc gtgaaacgga tgacaaagga
    29821 aaacaggatc aagttgattg ttgttggaga tgatgatcag agtatctatg gattcagagg
    29881 atcagagcca gcatacatca agaactttga aaaagaatat cctaccaaaa ctcttttctt
    29941 attaactaat tatcgttctc gggcagctat tgtccaagga gcaaatagat tgatcagcta
    30001 taataaaaat gatcggatac caaaggcgat gattccagca caaaaagaag caggtgttat
    30061 ggaagcctat ttgttttctg atactaaagc agaaagtcag tggctaatca aacaaattca
    30121 gacatttatt caaaaaaatg ggaaatataa ggaatcaatc atattatacc gatcagcttc
    30181 acaaacgcag cagcttgtcc aatctctgct gaaaacgaat attccttttg tactggaagc
    30241 agatagtcca tatgaaggta tctttggtat caagtctttc caacatttct atcaaaaaat
    30301 tgttcattgg caaacagcac tgaatctgca aaagaaaaat caggcctacc agcaattgct
    30361 ccggcagctg atggcagact gctacctgaa aaaaagtgaa tgcgatcaat acttttctcc
    30421 taaatatcaa aatatagccg ttgcagatta tattttgtcg ataagcccca atttaaaatc
    30481 gaaatctgca gaaatccgag agtttgagga agcgttgcgt aaaacagacc agcatcaaga
    30541 aatctattcg cttgttcaaa gctatcttca tctgccacgt atagctaagg aaatcgacca
    30601 gagtgatcaa gaatggatac gagaagaagt aaagcagcat gctactttca gaagactcca
    30661 agccttatcg caagaaaccc aagcgacagc taaagaatta aaaacccgct tgattgctta
    30721 cagacaagga aaacttgatg cactatgtat ccagagcatt cacaaaagta aaggtcttag
    30781 ttaccgaaat gtctttttga ttggttgtaa cgaaggaagt ttgccttata acggagcgac
    30841 agaaaagaaa gtaatcgact ttaatgaaat aaaagcagaa ccagtaacga cgatagaaga
    30901 agaacgaaga ctattttatg ttgcgatgac tagagcaaga aatcgtctgt atctatgtgt
    30961 acctcaaatg aaaaattcaa gaaaattgaa agtatcgaga tttatcaaag aaaccgggag
    31021 tcgtgtgaaa aaaggaaagt gatagaagag agggtgtgac agaagtggtc agtttcaaga
    31081 aagaagaagg aatgtacgaa aattgcttct caaatttttg tgaaatttca gcttattttc
    31141 gaagaagcta tttctggaac acagtttatt cggaaa
//
LOCUS       DAIGOK010000021        29107 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.021, whole genome shotgun sequence.
ACCESSION   DAIGOK010000021 DAIGOK010000000
VERSION     DAIGOK010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29107
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.021"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(<1..1030)
                     /locus_tag="NOA01_000729"
     CDS             complement(<1..1030)
                     /locus_tag="NOA01_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCI0631949.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="NOA01_000730"
     CDS             complement(1112..1801)
                     /locus_tag="NOA01_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCI0631950.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="NOA01_000731"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="NOA01_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCI0631951.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /locus_tag="NOA01_000732"
     CDS             complement(2328..2831)
                     /locus_tag="NOA01_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCI0631952.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3035..3162)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="NOA01_000733"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="NOA01_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCI0631953.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="NOA01_000734"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="NOA01_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCI0631954.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="NOA01_000735"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="NOA01_000735"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HCI0631955.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="NOA01_000736"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="NOA01_000736"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HCI0631956.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7462)
                     /locus_tag="NOA01_000737"
     CDS             complement(6443..7462)
                     /locus_tag="NOA01_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HCI0631957.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7635..8540)
                     /locus_tag="NOA01_000738"
     CDS             complement(7635..8540)
                     /locus_tag="NOA01_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCI0631958.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="NOA01_000739"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="NOA01_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCI0631959.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="NOA01_000740"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="NOA01_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCI0631960.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="NOA01_000741"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="NOA01_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCI0631961.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /locus_tag="NOA01_000742"
     CDS             9753..10397
                     /locus_tag="NOA01_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCI0631962.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="NOA01_000743"
     CDS             complement(10453..10956)
                     /locus_tag="NOA01_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCI0631963.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11553..12518
                     /locus_tag="NOA01_000744"
     CDS             11553..12518
                     /locus_tag="NOA01_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCI0631964.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12617..13789)
                     /locus_tag="NOA01_000745"
     CDS             complement(12617..13789)
                     /locus_tag="NOA01_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI0631965.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13776..14921)
                     /locus_tag="NOA01_000746"
     CDS             complement(13776..14921)
                     /locus_tag="NOA01_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI0631966.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14935..15867)
                     /locus_tag="NOA01_000747"
     CDS             complement(14935..15867)
                     /locus_tag="NOA01_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0631967.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15984..17063
                     /locus_tag="NOA01_000748"
     CDS             15984..17063
                     /locus_tag="NOA01_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCI0631968.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17060..17689
                     /locus_tag="NOA01_000749"
     CDS             17060..17689
                     /locus_tag="NOA01_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCI0631969.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17799..18026)
                     /locus_tag="NOA01_000750"
     CDS             complement(17799..18026)
                     /locus_tag="NOA01_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCI0631970.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18129..20717)
                     /locus_tag="NOA01_000751"
     CDS             complement(18129..20717)
                     /locus_tag="NOA01_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HCI0631971.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20737..21396)
                     /locus_tag="NOA01_000752"
     CDS             complement(20737..21396)
                     /locus_tag="NOA01_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCI0631972.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="NOA01_000753"
     CDS             complement(21445..21948)
                     /gene="trmL"
                     /locus_tag="NOA01_000753"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCI0631973.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22169..23035)
                     /locus_tag="NOA01_000754"
     CDS             complement(22169..23035)
                     /locus_tag="NOA01_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCI0631974.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23144..23785
                     /locus_tag="NOA01_000755"
     CDS             23144..23785
                     /locus_tag="NOA01_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCI0631975.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="NOA01_000756"
     CDS             complement(23847..25208)
                     /gene="mgtE"
                     /locus_tag="NOA01_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HCI0631976.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25226..26125)
                     /locus_tag="NOA01_000757"
     CDS             complement(25226..26125)
                     /locus_tag="NOA01_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCI0631977.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26127..26924)
                     /locus_tag="NOA01_000758"
     CDS             complement(26127..26924)
                     /locus_tag="NOA01_000758"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HCI0631978.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26902..27606)
                     /locus_tag="NOA01_000759"
     CDS             complement(26902..27606)
                     /locus_tag="NOA01_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCI0631979.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27737..28312
                     /locus_tag="NOA01_000760"
     CDS             27737..28312
                     /locus_tag="NOA01_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HCI0631980.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28430..29107
                     /locus_tag="NOA01_000761"
     CDS             28430..29107
                     /locus_tag="NOA01_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCI0631981.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8563 a         6169 c         5232 g         9143 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    11341 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    11401 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    11461 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa
    11521 tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca
    11581 atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa
    11641 aaaataagca tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga
    11701 ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa
    11761 tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg
    11821 atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggaa
    11881 atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt
    11941 tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa
    12001 aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga
    12061 caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt
    12121 tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag
    12181 gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt
    12241 ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg
    12301 tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga
    12361 aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt
    12421 caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata
    12481 tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc
    12541 tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc
    12601 tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt
    12661 tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat
    12721 tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat
    12781 tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca
    12841 agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt
    12901 tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa
    12961 tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg
    13021 aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt
    13081 aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct
    13141 ggtttcgttt actgatctct ttgcgattaa acgctagatt gatgacgctg aaaccgctaa
    13201 aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt
    13261 tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt
    13321 gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt
    13381 gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct
    13441 gtatcttgat cgtcgccatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat
    13501 gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc
    13561 catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag
    13621 gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat
    13681 caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat
    13741 tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct
    13801 ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga
    13861 ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact ttcgctgatc
    13921 aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca atacttcact
    13981 tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg
    14041 ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt acctaacaaa
    14101 atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg ttcaccaaag
    14161 tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt caaaacaatc
    14221 gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg aatcaaggac
    14281 aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg gaccccccac
    14341 atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact tttgaaactc
    14401 atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg
    14461 ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc aatacgctgt
    14521 aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg acccacatgg
    14581 agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc atttagcttt
    14641 tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc attgacttcg
    14701 acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc tttgattaca
    14761 attccagtat taatcgtttc aagattgcca ccttgatcaa tctcgccaaa ggccgccata
    14821 aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga tttatttcgc
    14881 agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc tcctctagtc
    14941 tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt ctttggttgc
    15001 tatttttccg taactgatct tttcttcttg taagattgta agaatcggca acaatactgt
    15061 atttagttca gtagttgtta cttggagctg acctttttta taagcgattc catcagaagc
    15121 tattgttcgc tgaatcgctg acaacacatt ttctgggagt aatgggatgt cgatcgtcaa
    15181 acgatccgcc gtccgtacca tatcttttag agattctttg gttcctttag caatcacctg
    15241 tccttgatcc atgatcacga tctggtcgca aagaatttct acttcttcca tgtaatgtgt
    15301 tgtatagaca atggttgctc cttgtcgatt cagctccttg atactttcta aaattttatt
    15361 ccgactttgg gggtctacag ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg
    15421 ggcaatccca catgcgatgt tcaaccgacg tttcaagcca ccactaagtt ctcctggacg
    15481 aaacgaaaca tatttttcta atcccactaa ttgaatcacc tcttcaatca aggctttcct
    15541 tttctctttt tcggaaacat ataaaccgca aaaataatcg atattttctt gcacacttag
    15601 ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc attgccttga cagcataatt
    15661 ttcaggagtc attgttgtcc cgaacaattt aatggttcct ttgtcataat ttaacaaaga
    15721 aagcatacag ttgatggcag tcgatttccc actgccgttt ggacctaaca gacctaagac
    15781 ttctccttct tgtacttgca gatcaaaatg attcaatgct acaaaatctc catagcgttt
    15841 gactaattta tcaatttcga taatcatcat gaaaaactcc ttccaaattt cttgttgatc
    15901 tgactataat aaagtgcaag aaaagaaact agtgacgatt ggctaaacca cacatgacat
    15961 ttgtcacaat gaaggagtct gctatgcata aatctcggct actcaacgaa actagctttc
    16021 tactggttat ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg caagttatct
    16081 atttattgat cgccgtttta ttcactggtg tcagttattt actgcctaca aagccaaatt
    16141 ggattttcca agttattcta tttcttttat gtgccttttt acctgatctt gtcctgtttc
    16201 tgccagcgac gatgcgagtg atcgcccctg aagaaagcca ccttgattac ttgtttttgt
    16261 taggtttttt gttgatttcg acggttggta atcatccccc catcaaggtc gcctttgtcg
    16321 taattttgct gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg accgatcttg
    16381 agaaaaagta cattgtctcc aaagatactc attgggaaaa acaacaagag ttatcggaac
    16441 aaaataaagc attggccctg tcacaagaaa cacaattagc cttgcaaatt gtggaagaac
    16501 ggaaccgaat tgcccgagac atccatgaca atgtcggtca tctgctgtca agtgcgattt
    16561 tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa ccattagaaa
    16621 atttaagtgc caccgttcac actgggatgg atcggattcg tcaaagtgtc catgacctcc
    16681 atgagacatc gatctctttt caacactcct tagagtttct gatcgctgat tttccttctt
    16741 ctgttacgat tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa aactgcttct
    16801 taatggtcat caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg
    16861 ttttacattt tcgaacgttg ccggcctttt atcgcttgca aatcacgaat gatggcacca
    16921 gcccacaaaa aaattctgct cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag
    16981 aacaaaaggg acaagtccat attttccaag aaaaccagca atttcagtta acgatcattt
    17041 taccaaggga gggacaccaa tgataacagt agtcattatt gatgatgatc catttgtgac
    17101 gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca
    17161 ttgtgccaag gatgcgctag tcttatacga aacccatcgt cccgatgttt tgctgacgga
    17221 catccgcatg cctaaacaaa cgggcatcga tgctgccaaa gaaattttgg ctaaatttcc
    17281 caaagccatc cttttgcttt tgacgacatt caaagatgaa gaatacatcc gcgaagcatt
    17341 ttccatcggt gtcaaagggt atttaatcaa acagaacttg caagcgatta ttccttctgt
    17401 caaatccgct tataatggac aggccgtatt tggtaatgaa atcatcgaga ctttcactca
    17461 aatgatgaag aataaaccga cgattgatag tcatgcttat gcttctttta gtgaacgtga
    17521 gcttgcaatc atcaaagaag tagctgccgg gaaaaacaat aaagaaattg cagatgcctt
    17581 gtacttgagt gacggcactg ttcgcaatta catcagccaa ttactagaaa agctagattt
    17641 acgggatcgc acgcaattgg ctatctattt ttatcagcat ttgcaataag tcttatctaa
    17701 cacaaaaaaa cctgaatgat gctccttcgc atcattcagg tttttttcac atcttctatt
    17761 caattgtccc gcaaaccatt tcctcacgat tgaaagaatc aaggattcgc agggctgcca
    17821 ggattcgttg gattgttcgg cgtgttaggc ggaaaaatag gcgccttgtc taacgttttt
    17881 tctagttttg attcttcacg atcgtctcgt tctacatcag gactttcatt caatttgaat
    17941 tctttttcca tctcatctaa ctttttaaag tcattgccag attcttttgc taatttcgct
    18001 aatttatctt cggtttcttt agccataaag taacgccccc cttttgattt cagcttagca
    18061 tatcacactg aaaaaacaaa agaaaacgct aaccaacgat catttttagc tatttctcat
    18121 ctttaaaatc aagcattttt tgccggtata aaatcgtagg gagaactgtt ttccatacca
    18181 atcatcggat ctactaactg gttatcgatc acttctgctg taagaacgaa atctttagaa
    18241 acagcaggtt ctacctcttc tttcttttct tctccaacaa atgaagaaaa cagatctggt
    18301 tccactgttt ccattacttg gtttttcgtt tcattcgtcc cactgtcatc ttctacaggt
    18361 agcttttctt ttacaacccg tggctctttt gtatagttcc catcttcaaa tggatcattt
    18421 ccgaccaaag aatcttcata agctgctagt ttggtccgga gagtgatatt cattttatca
    18481 ttatccatga tccttgattt caacgtagtc atccggcttg ctgattcatt cgctacacaa
    18541 gtgacatagg cttgctcttc ccctaacaaa atcaacagtt ccttactgcg agtcactgcc
    18601 gtgtagagca aattacgctg aagcatccgc tgatattgat ggaccatggg caaaataacc
    18661 atcttgaatt cactgccttg tgatttatgg attgagcaac aataagaaag agtgatttta
    18721 ttccattcgt ttcttttata agtgacctca tttgcatcaa actggataac taattcatcc
    18781 actttatctt cagaatcttt tgccaagatg atacccgtga tttctcccat atctccattg
    18841 aatacattca attcgggagt attgactaat tggaggactt tgtctcctat ccgataaacg
    18901 gtgtcgttcc aatgtacttc cttttttcca ggtttccctg gattgaatat ctcctgcatc
    18961 attttattta acgcatcgat ccctgcaggt ccccgataca ttggtgctaa tacttggata
    19021 tcttgtgcac ggaatccctt atttttcgct ttttccacaa ttttagagat caagggttca
    19081 atttgataag catcacttcg gaaataggaa cgatcttttt gattctttgt gaaatctttt
    19141 ggcagacgtc cttctttgat ctcatgggca agaggaataa tactagaacc atcaccttgc
    19201 cgataaatct gattgagttc cattttggga atttgttcga tttccaaaag atcatggagt
    19261 acttgaccag gaccgacaga gggaagctga tctttgtctc ctactaagat aacttgcata
    19321 ttcgtcggaa tcgctttgaa taatgtgtta gccagccatg tatcgaccat agacatctca
    19381 tcaacaatca aaagtccgcc ttctaattct ttcgtactga ctgaaggtgt tttttcccgt
    19441 ccattcagcc ccagaagacg atggatcgta ctggcaggca gacctgtcgt ttcgttcatt
    19501 cgtttagctg ctcggccagt tggtgctgca agtagaatag gaaaaatttc ttgggtatat
    19561 tttgaaggat ccaattccag tccgttcaac tctgcaaaaa gattgacgat tccgttgatc
    19621 acagtagttt tccccgtacc tggtcctcct gttaaaataa atagagggga acggattgct
    19681 tcagcgatag cttgttcttg cgaaggtcca tactgtatgc cgaatatttt ttcgatacgc
    19741 cgaatattct tctgcacttc tttttcagga taatggattt cttttttacg actgagaagg
    19801 cgctggatcg aattaccgat cccccattca gaaaagtaca aactgttttc gtaaagatta
    19861 gtctcttctt gttgtatctt tccttcttca acaagttgga taattacagt agcaacctga
    19921 tcaggctgga tttcaattgg gcgacttgtt tctaaaatac ggatcgtttg ctgaagcaat
    19981 gtctccgccg gtacgtatgt atctcctgtt tgaactgcat gctgaaaaat ctgatgcaag
    20041 atagctgctc gaattcttct cggtgaatct gctgcaatac cgatttgttc ggctagatta
    20101 tctgcacgtt tgaacccgat tccttctatg tcttcgacta gctggtacgg attttcttcg
    20161 atgatatcca aagactcgtt cttataggcc tggtagatac tgaaagctaa ctgactaccg
    20221 aacccgtaac gattcagtcc catgatcact tgatccatcc cgtggtttag ccgaatcgtc
    20281 tcagcaagca tttctctttt tggcttcgtc aaaccggaga tttgttttaa taccgaagga
    20341 tcagcaatga ttttttcgat agcatcttct cccagcaatt ctacaatttt ttctgctgtt
    20401 ttcttcccga ttcctgggaa tttttcgctg gaaagatacg ctacgatccc atttgcagtt
    20461 gttggctttt cttgctcata tctttcgagt ttaagctgcc ttccgtattt aggatgatcc
    20521 actaattctc cataaaaacg ataagtctca ttttcttgaa tatcgccgaa acttccggtg
    20581 actacaatct ctttttcacg gtaatcagct gtggtttcac tgatgtgtac caaaagcact
    20641 ttgtaaaaat tactaggatt ttgaaaaaaa atcgcggcaa ctgtaccaac gaaataaggc
    20701 atttcttctg gctgcatcgg aagccgcctc cttcttctat agtaaaggtt ctggtgctgt
    20761 ttcagttcct aaaaaatctg tttggttcca tactttcagt tcatatggca ataatctgca
    20821 aggctcttca gtctctaaaa tcgtcaaact actattgaac aatccaccca tttcacgcaa
    20881 ctcacttagc tcttttcctg tcatccattg aatggctgca gttaaagaag ctccatgtcc
    20941 tacgaataaa agtgggccat ctttatgtcg ggcaaccgca tctgctacaa cagtttcgat
    21001 acgtgtgatt gccgattcaa tcgtttctcc gtgaaaagga gaaggatcat acagatctaa
    21061 atgattacgc aaattcggca gattatccgg atatttctgg tacatttcat cgatcgactg
    21121 accttctaaa gtacccagcc ctagttctct tagcttatct gtataaaaaa tctctaccgg
    21181 acgagcaagc tctttgttga taccttctgc tgtatcacgt gctcgctttg ctgtactgga
    21241 ataaattgct ttaaaaggaa catattttac ctttttccca aatgctttga tttcttcgta
    21301 actttgtggc aaaagcggag aatcgccatt gctcccttga aaacgtcttt cctgattcca
    21361 ttgtgtcttc ccatgtcggg taaaatatag ttccatacgc agcaaactcc tgtctttctt
    21421 ttattttact ctataataaa ctgtctaatc cagcttgtca ttttcatagt gatgcgtcaa
    21481 ttctaaatta gggaatcctt gctggcgaag tgcttcatac actatcattg cagctgtatt
    21541 cgataaattc aatgagcgga catgctcatc attcataggg atgcgtaaac acttttcttc
    21601 gttttcacgc ataaatgttt ctggcaaacc ggttgtttct tttccaaata aaaagaagtg
    21661 ctcttctcca tcagcaaaat tttcgtcact atatacttta tgggcaaatt ttgagatcaa
    21721 atgtagttta cgattaccta aaaatgttaa aaaagcttct aaattactgt ggtaagtaat
    21781 atttacatca ttccaataat ccaaccctgc ccgttttaga tgtttgtcat cagttgaaaa
    21841 acctagtggc tcgattaaat gaagcggagt attcgtggct gcacacgtac gggcaatatt
    21901 tccagtgttt gcagggatta atggttcaaa taaaacgata tgattcatca ggatcattcc
    21961 tctcatattt atgttcgttt ctattttctg acaatttgat ttttgcataa aaaaaaacgt
    22021 atcaactcgg tgtcagtcac tgcgagtcgt tacgttagtg aagctcttct cactaacttt
    22081 tatcagaaca ggaacctgat aaaaaccaat gaaaaaacaa cttgttgcta atataacacc
    22141 ctcaactttt ttttgcaacc tctactcatt aaaaactgct agaaaaacgt tttctcttag
    22201 ttccttttaa taattctatg tcaattgtaa tcttcttcaa aggattctta tataagtctg
    22261 ctttgattcg ctcttctacc tgccattcta aaggtgacat ggccaaaaga tctttttgat
    22321 tttcctctgg tatctcaaat tcataggtta cacgctgacg ttcagttaat ttcattgctt
    22381 cagcaaactt ggctactaca cgttcattgg aataactctg tttttcaggt tgattcggat
    22441 aaaaagcctg acgtagttcc tttaaataat tttcttgtgg caccaccttc aacacttctc
    22501 catcttctga gagtacacgt tggaattctt ggtaatgaga aggcgaaaag atatttaaaa
    22561 ccgcgttgaa tgtttgatca gcaaacggca aattggtcaa atcagctaca caccaaaatg
    22621 cttcggcaga ttgttctgta gctgcgtaga ctccttcttt tgaaatatca aatccaacag
    22681 cattttgaat ctttttcaat tttgaaagtt cattcaagaa gctaccttct ccgcagccaa
    22741 catctaatag cgagacttct tcaggtaaat gctgcgcaat agtctcaata acgggctgat
    22801 acatgccaga ttggatcatt ctacgccttg gaacgaacat tccttgatcg tattctgttt
    22861 taaccgcatg ggataaaaaa taaagtgttc cttttttgga aaggtcaaaa cggtgattat
    22921 ttttgcatat caagccatta tcagactttt ttacttcttc ttggcaaacc ggacagcgga
    22981 acagctgatg attttctgcc aaaaactggc gtcctcggtc aatttttttt aacatagttc
    23041 cccctcattt ctgttttatc ttaccgttag atgctcactt tggcaactgc taaaaatatg
    23101 atacaatgcc tagaagaaag tgaaagataa aggagaagat tagatgatca aagaattttg
    23161 cgctgaaaac tacacatcta ttcctctagc aattgctaga ggagccgatc ggatcgaatt
    23221 gtgtgacaac ttagcggtcg gtggcacaac cccgagtact ggtgtgatcg aagaaacgtt
    23281 aagttatgct ggggaaaaag atatccccgt aatgacgatt attcgtccta gagggggcga
    23341 cttcgtctat aacgatatcg aattgaaaat catgcatact gatttgattg aagcaaagaa
    23401 attagggacg gatggcgtag tatttggttg tcttactcct tcaggctggc tagacgaaga
    23461 ggccttagaa ctattgatcg aaacagcaga agggttgcag atcaccttcc atatggcttt
    23521 tgacagtatt cctgaagatc ggcaattcga agcaatcgat tggctggtag aacatggtgt
    23581 tgaccgtatc ttgactcatg gcggtcctgc tgacaaaact attgtagaaa atttcccacg
    23641 gcttaaagaa ttgattgcct atacagatgg ccgcttaatc attttgcctg gtggtggtgt
    23701 cactgcagag aatgcagcac atgtagctaa agaattgaac gtaagcgaag tacacggaac
    23761 caaaatcgtg gatcttcagt tataatctta taaacaaaaa aacatttgca acaggcataa
    23821 tgcttattgc aaatgttttt gtttttttac ataaaatagc ccataaataa agaagcaata
    23881 ttgaaataaa tcagtacact cgtcaaatcg ctcaaagtcg tgataaaggg accgcttgct
    23941 actgctggat caaatcctag acgatccatt agcatcggga ttaaactacc tgcaagattc
    24001 gccactgtga ttgcaaatag catagccatc cctatgacaa accccaagat aaaattatgt
    24061 ttccaaaaac ctacgattag aaaaatcgtg atccccgtaa cagctcctgt gacaacgccg
    24121 gtcaagactt cagccaaaac aagtcgaaca aatccatgat ctttctcatc agaaatcgca
    24181 agtcgacgaa cagccacagc tagcgactgg gttccagcat taccggcagt tcctgtaatc
    24241 agcgaaatga agactgctaa aatactcgct tcacttacca agtcttcata gtgactgatt
    24301 aaagaagcgg tcgacatacc taaaaacaac agagtgatca gccaaggcaa acgtcgagac
    24361 gctgccttta ctggattttc attaatttct tccacattga ccccggcaag tcctgaataa
    24421 tcgctagcag cttcatcatc aataacgtcg atgatatcat cgactgtgac gattcctaac
    24481 aaatgatcat cataatcggt aacaggaaga gctaagaagt cataatctct aaatgtttgt
    24541 gccacatctt cttggtcgtc gcctacatga actgaaagca ctcgttcgct catcagatca
    24601 gaaatcattg cgtcgtcatc attcacgatt aggtcgcgca gagaaataac ccctactagc
    24661 tgaccttctt gattaacgac atagatgtaa tagattgttt cagctacatc tgcttcgttc
    24721 ttcagcacgt acatcgcaga gcgaacagtt tgattggcta cgatcgaaac aaattcagtt
    24781 gtcatgatcg cgccagccgt ttcatcctcg taatggagaa gttccttgat ttcactggca
    24841 tcgtctgccg acatcaaact taaatattta gcaatttgct ttttatccag cgtattaagc
    24901 aagtcaacag cattatctgt atacatctca gaaagcatat cagctgcata gctaggacgc
    24961 atttcatcta ggtattcttt catatgttcg ttgtcttctt caatcacatc gaacatatcc
    25021 gctaattctt taggcgacag ataggaatag atcagctgcc tgttctcttc tgtaagagat
    25081 tggtagaatt gcccctgctc atagatatgc atctctaaaa aactgtttct gaatgcctga
    25141 atattatttt cccttaaatc ttgagtcaac tgggcaaatc tttcctctaa ttcttgattt
    25201 tcatccaaac caatctcacc tcgccttatt cttcaaaccg tctcacaact gcctccatgt
    25261 ctgcggctaa aggctgcttg aactccagca actcttctgt aaatggatgg taaaaacgca
    25321 gatcataaca atgaagggct tgtcttttta ttcccagatc cattcgcccg ccatacatat
    25381 catcaccgag cagggcacag ccaattgcgg aaaaatgaac tcttatttga tgtgtccttc
    25441 ctgtatgcag ctggatatca actaacgcga tttctgttag tcgcttctct atccagtact
    25501 cagtctgtgc cgaacgtcct tcatcagaga ccattcgctt caatagcgaa gaataatctc
    25561 gggcgatcgg caaatcgata tgtccatgtg ccgataaatc tgaaagcctt ccactgacta
    25621 aggcctgata ttttttcaca aacttctttt cacgaagttg catatctaat tttgcgtgcg
    25681 caaatccatg ctttgcaaaa agcatcaagc cagatgtatc ccgatctagt cgcgtcacca
    25741 catggattac ttggttttcg taaccttgtt ttttataata agcctttaca cgattagcca
    25801 ttgtcccatt tggatggtat tgggcaggaa tagacgaaac gcctgctggt ttatttacta
    25861 ccagaacgtg ttcatcttca tacacgatat cgatcggtgt ttcatccaaa agtacggttt
    25921 catgttctcc ttcagcagga atcacaatgg ttaccttatc atccttagct agagaaaaca
    25981 agacattttc tacttgatca ttaactttga tttgaccgcc ttgaaatttt attttcgcta
    26041 acagaccctt tgagatccct ttttctttta aaaaatattt tacttgctga gaatgatctt
    26101 tctcataatc ccatgtaaac tgcatatcaa tcctcactaa taaaggcatc tttcacacga
    26161 tgccaaaaat gcatatgtcg ataagaagca aaatggatcc gttcttctgc tattcgataa
    26221 taaattgagc ggatatttgc ttttgcgaca ttcaattgat caaccgttac aagataatct
    26281 tctgtatttt ccaattttat ttctagccat tcatcatggg caatcacgat cggagaaccc
    26341 aaagtacgaa acactcgatt attcagtgaa gcaatctctg ccaactgaaa ggcattgata
    26401 ctaggatgga tcacagcacc gccgacactt ttattatatg ctgtcgaacc agtaggcgta
    26461 gaaatcgtca atccatctcc tctaaaactt tcaaacagct cgtttttgat gtatacatcg
    26521 gctaccatcg tacgattggc ccgtttaatc gttgattcat tcagtgctaa aaaatgctgg
    26581 tccggtgttt catccaaata actgatccga acatccagca agggataact aacactttgt
    26641 tcccggttcg ttcgtaagct gtctactagt tcttccagct cgtaatcccg ccaatccgtg
    26701 taaaacccta aatgacctgt atgcacgccc aagaaacgca catcattcag acaatgactg
    26761 tagtgatgga acgccgacaa taatgtgccg tctccgccga ctgagatgac caattctggc
    26821 tcatgatcat ctcgttgaat ccctgcttgt tctaaaagtg cgttcaaacg ttctgtcact
    26881 tctatagtct ttgcttcttt attatgaacg atcgctactt tcatcccctg cctcctctct
    26941 ttcgctttct ttcttcaaaa gcgttcgata gtagacatca tctgattttc ctcttccatg
    27001 ggagaataac cgctgtgctt cttggatttc ttcacggata ctggacatct cttcatccaa
    27061 ctgataggca gcttctgccg cacgctgaag tcgttcactc atttcttctg gaaaaacgcc
    27121 ttgatattta taattcagcg aatgctcaat cgttgcccag aaattcatcg caagagtccg
    27181 aatctggatt tccgccaaaa ttttcttctc accgctgatc agctgtacag gatactcgat
    27241 caccagatga taagaacggt agccacttgc ttttttattt gtgatgtaat ctcgttcttg
    27301 gagaactttc atgtcttttc ttttacggat gatctcaaca acttgatgga tatcttctac
    27361 aaactgacac atgatcctta acccggcaat atcagacatt tcttcttcta aacgtcccat
    27421 tggaatatgc cgtaattgtg attttgttac aatactatcg actggcttga ctctcccagt
    27481 gacaaattca atcgggacat gtcgattttg ttcacggaac tgtttgcgga tcccccgcag
    27541 tttcactttc aattctgata cagtttgttc atatggtgct aaaaatgctt cccaatctcg
    27601 ttccatagct gatacctcta cttcattaac attcattgtt tattctacca tatatgcaaa
    27661 gttttttcgt actcttgatt tatgcattga ccaaaaaaag aaaattaggc aaaatagaca
    27721 aaaaggagtc gatcaaatga cacaaatgct tgaaatcgaa tataaatcta tgctgacaaa
    27781 gcaagagtac gagatattca tcgctcacta tcaattaaca gataaagatt tccgtgtaca
    27841 gactaatatt tatttcgata ccgttgatga acagctgaag aaacaaaatt gtggcctacg
    27901 gatccgtttc gttggtcatc aagctgaata tacattaaaa acacctgctg aaaaagggcg
    27961 tctcgaaaca acggatacct ttataacaga agaagcagaa gcatttatct tcgaaaaacg
    28021 tctgcctcgt tcaggcgcag tttttcaaaa actttctgat ttgaatatcg atcctgcttc
    28081 attaatccaa accggaaagc ttacaacaaa acgagctgaa ttccctattg aagaaggact
    28141 tttagctatt gatgaaagct ggggagacaa tctacacgat tttgaactgg aactagaggt
    28201 aggggatgca tctgtgggaa aaattgcttt tataaatttt ttaaaacgtt tttctttacc
    28261 ttatcgtcct gctaagaaca agatccaacg tatgcttgaa gctaaaaatt agaaaccttt
    28321 cattttcaca aggcacttga gttcattttt ttactttttt ctgttaagtt ataggatgtg
    28381 aaagaatcta gtcactagtt gtatagaatc tattttgggg gagattcata tgatcgaaat
    28441 ctatttattt gttaatcctt taggggaata ctgtttcgaa atggaacaac agttattaca
    28501 atttatcgaa gaagagtacg ggacaccatc aaaagaaaag atgcaatttc gctttcttcc
    28561 tttggtcaat ttgcagacaa ttggggatgt catgcaacaa aaaggaattt ctcagaacaa
    28621 tcttgaggaa cgtaatcact tattctcaat gacttattct gcagcattgg actgcaaagc
    28681 ggcgcagttt caaggaaaga aaaaaggccg ccgttttttg atcaaattgc aagaagcagt
    28741 cggctgtaat gacataccat attctcaaga acttgctgaa tccatatttg aagaaatcgg
    28801 cggcgatttg aatatgttta aagaagatcg tcagtctgac ttcgttaaag aagtattctt
    28861 ttctgatcaa aaaattgctc gggaaatggg catcacaaaa cacccttctg cagtcgttta
    28921 caattatgcc tgcgaccgtg attttggtgt tttagtagaa gtaaaaggta caagtagcct
    28981 ttggagaatc acagagctgt gtaaaacaac agataactca tatcaagttt ttaatggtga
    29041 acatttacat acaaatgatt ctcagttcca ttcaagaact agccatttac acttgctttc
    29101 taattaa
//
LOCUS       DAIGOK010000022        28818 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.022, whole genome shotgun sequence.
ACCESSION   DAIGOK010000022 DAIGOK010000000
VERSION     DAIGOK010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.022"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            123..1394
                     /locus_tag="NOA01_000762"
     CDS             123..1394
                     /locus_tag="NOA01_000762"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCI0631982.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            1391..2686
                     /gene="rho"
                     /locus_tag="NOA01_000763"
     CDS             1391..2686
                     /gene="rho"
                     /locus_tag="NOA01_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCI0631983.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            2785..3048
                     /locus_tag="NOA01_000764"
     CDS             2785..3048
                     /locus_tag="NOA01_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCI0631984.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            3493..3942
                     /locus_tag="NOA01_000765"
     CDS             3493..3942
                     /locus_tag="NOA01_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCI0631985.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            3999..5417
                     /locus_tag="NOA01_000766"
     CDS             3999..5417
                     /locus_tag="NOA01_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCI0631986.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            5417..6277
                     /locus_tag="NOA01_000767"
     CDS             5417..6277
                     /locus_tag="NOA01_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCI0631987.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            6451..6561
                     /locus_tag="NOA01_000768"
                     /pseudo
     CDS             6451..6561
                     /locus_tag="NOA01_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
     gene            6845..7510
                     /locus_tag="NOA01_000769"
                     /pseudo
     CDS             6845..7510
                     /locus_tag="NOA01_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
     gene            7657..8022
                     /locus_tag="NOA01_000770"
     CDS             7657..8022
                     /locus_tag="NOA01_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HCI0631988.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(8170..9378)
                     /locus_tag="NOA01_000771"
     CDS             complement(8170..9378)
                     /locus_tag="NOA01_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCI0631989.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(9397..9675)
                     /locus_tag="NOA01_000772"
     CDS             complement(9397..9675)
                     /locus_tag="NOA01_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0631990.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            9829..10524
                     /locus_tag="NOA01_000773"
     CDS             9829..10524
                     /locus_tag="NOA01_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCI0631991.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(10966..11763)
                     /locus_tag="NOA01_000774"
     CDS             complement(10966..11763)
                     /locus_tag="NOA01_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HCI0631992.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(11760..12944)
                     /locus_tag="NOA01_000775"
     CDS             complement(11760..12944)
                     /locus_tag="NOA01_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HCI0631993.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(12969..14216)
                     /locus_tag="NOA01_000776"
     CDS             complement(12969..14216)
                     /locus_tag="NOA01_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HCI0631994.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            14431..16176
                     /gene="dnaX"
                     /locus_tag="NOA01_000777"
     CDS             14431..16176
                     /gene="dnaX"
                     /locus_tag="NOA01_000777"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCI0631995.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            16196..16510
                     /locus_tag="NOA01_000778"
     CDS             16196..16510
                     /locus_tag="NOA01_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCI0631996.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            16535..17131
                     /gene="recR"
                     /locus_tag="NOA01_000779"
     CDS             16535..17131
                     /gene="recR"
                     /locus_tag="NOA01_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCI0631997.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            17195..17839
                     /locus_tag="NOA01_000780"
     CDS             17195..17839
                     /locus_tag="NOA01_000780"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCI0631998.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            17854..18183
                     /locus_tag="NOA01_000781"
     CDS             17854..18183
                     /locus_tag="NOA01_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HCI0631999.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            18197..19135
                     /gene="holB"
                     /locus_tag="NOA01_000782"
     CDS             18197..19135
                     /gene="holB"
                     /locus_tag="NOA01_000782"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCI0632000.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            19171..19995
                     /locus_tag="NOA01_000783"
     CDS             19171..19995
                     /locus_tag="NOA01_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HCI0632001.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            19988..20335
                     /locus_tag="NOA01_000784"
     CDS             19988..20335
                     /locus_tag="NOA01_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HCI0632002.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            20404..21276
                     /gene="rsmI"
                     /locus_tag="NOA01_000785"
     CDS             20404..21276
                     /gene="rsmI"
                     /locus_tag="NOA01_000785"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCI0632003.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            21385..22506
                     /gene="dinB"
                     /locus_tag="NOA01_000786"
     CDS             21385..22506
                     /gene="dinB"
                     /locus_tag="NOA01_000786"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCI0632004.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(22560..23162)
                     /gene="nrdG"
                     /locus_tag="NOA01_000787"
     CDS             complement(22560..23162)
                     /gene="nrdG"
                     /locus_tag="NOA01_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HCI0632005.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(23477..25666)
                     /gene="nrdD"
                     /locus_tag="NOA01_000788"
     CDS             complement(23477..25666)
                     /gene="nrdD"
                     /locus_tag="NOA01_000788"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCI0632006.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            25965..26759
                     /locus_tag="NOA01_000789"
     CDS             25965..26759
                     /locus_tag="NOA01_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0632007.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            26749..28791
                     /locus_tag="NOA01_000790"
     CDS             26749..28791
                     /locus_tag="NOA01_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI0632008.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
BASE COUNT         9199 a         5210 c         6029 g         8380 t
ORIGIN      
        1 ttttgaagtc gctctccatt cttttttact taaaacggca gaccatgctc gtttcttttt
       61 tttatctatg ataaaataca agcatatcca tgaaaaaaga ttaataaaca aggtggaaac
      121 agatgaaaaa aatcgtaatc aaaggaaacc gacctttagc tggtgaagta acgatcagcg
      181 gtgcaaaaaa tagtgcagtc gcattgattc cagctgcaat cttagctgat tccccagtga
      241 tcttggaagg tgtgccggac atacaagatg ttcattcatt aattgaaatt ttagagatca
      301 tgggcgcaac tgtccatttt tccaacaaca tactagaaat cgatcctcga gaagtcgtat
      361 cgattccgat gccgcacgga aaaatcaata gtttgcgtgc ttcttattat tttatgggaa
      421 cactgcttgg taaatttggc gaagcggtag taggcctgcc aggtggctgt tatttaggcc
      481 ctagaccgat tgacctacac gtcaaaggat ttgaagctct aggtgctgaa gtgacaaatg
      541 aacatggtgc catgtattta cggacagaaa ataaaggatt gcgtggcaat cggattttca
      601 tggacgtggt ttcagtcgga gcaacgatca atgtgatgtt agctgctgta aaagcaaaag
      661 gacagacagt aattgaaaac gctgcacgag aaccggaaat catagatgtg gcaactttac
      721 tgaataatat gggggcaaaa gttcgaggag cgggtacaga tgtgatccgt atcgaaggag
      781 tcgaaacatt acatggctgt cggcatttca tgattcctga ccgcatcgaa gcaggaacct
      841 atctagcatt agcagctgca gtaggaaatg gtatcaaagt gaaaaatgtg atctttgaac
      901 atttggaaag ctttatagca aaattgcagg aaattggtgt ccatatgaaa atcagcgaag
      961 atgaaatcga agtttacccc tcgaaagaat taaaacccgc aaacatcatg acctatcctt
     1021 atccaggatt tgccacagat ctgcagcagc cacttactgc gttactattg atgactacag
     1081 gcacttcaga aatcatcgat acgatctatg cgaaacgtgt gaaccatgtg ccggaactag
     1141 cacgaatggg cgctgatatt accgtggaag gaaatatgat catcgtgaat ggtccaaata
     1201 aacttcacgg cacagaagtc gtggcttctg atcttcgggc aggtgcttgt ttagtgattg
     1261 ctggactatt ggcagaaggg acaacgacga tttacaatgt agattatatc ttacgcggtt
     1321 atgatcatat tatcgagaaa ctaacagcct taggggcatc aattgaaatg atagaggaag
     1381 tgggagctga atgagcgatt atttgacaat ggcagaacta gagaacaaaa cgttaaaaga
     1441 agtttatagt tatgccaaag aatttaaaat tccttattac agtaagatga ataaaaagga
     1501 attatctcta gctgtgattc gtgcacaagc agagaaacaa ggattttatt atatggaagg
     1561 aatattggat atcgttggtc aagatggtta cggtttttta cggccaatca attatggacc
     1621 aagtgcagaa gatatctata tttctgcctc acaaatcaga cgattcagtt tgagaaatgg
     1681 ggacaaagtg gcaggaaaag cccgtccacc taaagaatcg gaacgttatt atggtttgat
     1741 gcacgtggaa agcgttaatg gaaaagatcc ggaagaagca aaagaacgtc cgcattttcc
     1801 agccttaaca cctctttatc cgcaaagaca gcttcaactg gaaacaacac cgcaaagact
     1861 atctacccga atgatcgata tcttttctcc aatcggtttt ggtcagcgtg gattgatcgt
     1921 cgcaccgcca aaagcaggga aaacttctgt attaaaagaa atcgcaaatg gtatcaccga
     1981 aaactatccg gaagtagaat taatcgttct actgattgat gaacgaccgg aagaagtaac
     2041 ggatctagaa cgaagcgtga aaggcgacgt ggtatcttct acatttgatc tgcagccgca
     2101 aaaccatact cgagtttctg aattagtatt ggaacgcgca atgcgtttag tcgaagataa
     2161 acgagatgtc gttatcttga tggatagtat tactagactt gctcgggcct ataacttagt
     2221 cgttccacca agcggacgta cattgagtgg cgggattgac ccagccgcat tgtataaacc
     2281 taagaaattt ttcggggcag caagaaacat cgaagaaggt ggcagtttga cgattttagc
     2341 gaccgcttta gtcgatacag gaagccgtat ggatgacgtg atctatgaag aattcaaagg
     2401 aactggaaat ctagaattac aactttcacg tgaattatct gaacgtcgga tcttcccagc
     2461 catcgatatc aaaaaatcag gtacccgaaa agaagaatta ttgatgccaa gtgaagaatt
     2521 agaagaaatt tggaaacttc gaaaaaatat gactggagat tcattagaat atacggaaca
     2581 actgatccgc tttttgcgta aaacaaaaaa caatcagcaa tttttcaaag aatttcaaga
     2641 tgtctccttt ggtaaaaaaa atccgaccag aaatctgaaa agataattgc aaccttgact
     2701 gaaacatgtt aagatgttaa tgttgtaaat cgacaaatca actctggttc agcaaatgga
     2761 gcagggcaaa ggagcgaaaa aatcatgaaa gaaaacatcc atccagatta ccacccagta
     2821 gtattcatgg attcaacaac tggtttcaaa ttcttgtcag gttcaacaaa aacttcacaa
     2881 gaaacagttg aatgggaaga cggtaataca tacccagtca tccgtgtcga agtaacttct
     2941 gactcacatc cattctacac tggacgtcaa aaattcacac aagcagacgg acgtgtggac
     3001 cgtttcaaca aaaaatacgg tctcaaagac gcaaacgctg cggaataata aaaacgctga
     3061 taactcaacg tttagcagac tatctgatag ggatagtctg tttttttatg ttttttgcca
     3121 atttaaaact tcgtttgatg ctgaaaaagt cgtaggatca taatgcacaa aacaatgaaa
     3181 aactagtata tagttttaat tatccacttg ataaaaacac cttatattaa cgaaatgaag
     3241 aggggttatt tcgaagtaaa aaaccattta cctaaaatta gaagctaagc tttcctttca
     3301 aggaaggctt tttgttttgt tatagacttg cgattgtttg tgagctagat cgttcatttt
     3361 aatgacttat acacgcggat gatgggtctt tttcctctgt tcattcataa tattgaacgg
     3421 tacagctagc gatgctatac tgacctcagt tccaagaaaa cgttaacatt ttttgtgggg
     3481 gagaggtttt ttatgaggtt tgatcaattg atcgcagaag aactgattgt tttcgatcca
     3541 tcgatagatt caaaaggatc attatttgag cgaatagggg atgtgttgaa aaaaacaaat
     3601 aaggtaagaa atgttcaaaa agtcgttaag gcactattca aacgagagga ggagatctcc
     3661 acaggaatag aagatggatt tgggattccc catgcaaaaa gcaaatatgt gacaaaacca
     3721 gcactgtgtt tttttcatac agccaaatta acggactata gcgggataga tgaaatgcca
     3781 attgaatata tttttgccat cgtagttcct gaaaaatcag cagatatgca tctagagatt
     3841 ctcagcacac tctccagacg attgatgaat gcagatttta gaaagggaat aaaagaagcc
     3901 actacgccag cagatatcat cagacagatt agtcaagaat gaaaatagta gagtctttgt
     3961 ccgagtatcg atagtgaaga aaaaggagga aaataatcat gaaaatcgtt ggtgtaactg
     4021 cttgcccgac tggaattgct catacttata tgtcggcaga aaaattagaa gtaacagcaa
     4081 gaaatctaaa ccatcaagca aaattcgaaa cacaaggtgt aaaaacagaa aatcagttat
     4141 ctgaacaaga aatcaaagag gcagatgcaa tcattcttgc agtagataaa gaaatcgaat
     4201 tggaccgatt tgccgggaaa aaagtaaagc gtgtctctac aagccgagca atcaaagaac
     4261 cgcaagtagt aatagacgaa gcattgagag atattggcgt attcgttgtg tctaatgaaa
     4321 aagaaccagc tgcaaatgag aaaaagccga caatctataa tcatttcatg aatggtgtga
     4381 attacatgct tccatttgtg atcgcaggcg gaatcatcat cgcactgagt tttgcctttg
     4441 ggattacagc ggctgatccc aactcagcag actataatgt gttagcagct gcattttcaa
     4501 ggatcggagg agatacagcg tttgccatga tggttcctgc gttaggcgcg gggattgcta
     4561 catcgattgc tggaaaagca ggttttgccc caggaattgt tgccggatta ctggcctcta
     4621 caggtggttc aggttttctt ggcggaatga ttggtggcgt gctagcaggg tatatctgtg
     4681 attttctggc aaataaaatt agcgtaaaaa aagaattttc tgctatttat cagctgattg
     4741 tcgtcccctt tttatcgatc ttaattattg ggcttttgat ggtatttgtg attgaacaac
     4801 cgatcacatg ggtgttggat gctttgacaa attggctgaa tagcttggga aatacatccg
     4861 ggctattgtt cggattactt atcgggatca tgatggcagc tgatatgggt gggccgatca
     4921 ataaatcgac ttcgactttc tcgatcggtt tgatgtcagc tggagtaaat gcaccgattg
     4981 ctgcttgtat ggctgcagga atgacacctc ccctaggtat tgctttagcc actttattat
     5041 tcaaacataa atttaccaaa gaagaacgga cttcaggtca atcatgctgg attttaggcg
     5101 cttcgtatat tacagaaggt gctataccgt ttgcggtatc tgacccgatc cgcgtgatac
     5161 ctagtttgat gacaggttca gcagtcgctg cagggatttc catggcagta ggagttactt
     5221 caatggcacc tcatggaggg atttgggtga tgctaattcc aaatgttatg aacaatttac
     5281 ctatgtatgt cttggcaatt gcatcaggta cagtcactac agcctttatg cttggactat
     5341 tgaaaaaaac agtgaaagag aaaaaacaag acaagtggac gaaaaatgaa aatctaacaa
     5401 atgaggtgga agcgtaatgt tatatacaat gaaagagtta ttgaaagtgg ctaaagagaa
     5461 tcaatttgca gtaccagcct tcaatatttg cagctatgat atgatgaaag caatcatcga
     5521 agaagcagag agattagaag caccagtaat cttagaaatt catcctgatg aaatcgccta
     5581 tttaagtgat gagtttgttg cggcagtaag agcctatgct tatacaagta aactgccgat
     5641 cgttatccac ttggatcatg gcggtacgat agaagatgtc ttacgggcga ttcgaaacgg
     5701 ctattcttcc gtgatgatcg atgcgtcttt gaaatcattc gacgacaatg tacagattac
     5761 gaaaaaagtg gttgcgttag ctcatgcggt aaatgtctca gtggaggctg agcttggaac
     5821 gattgggaat aatggttctg ctgaaggagg tgctgaaaca atcatttata cagatcctga
     5881 tcaagcgaag atttttgtga aagaaacagg aatcgataca cttgctgtag caattggaac
     5941 agcgcatggc ttgtatccga aaaacagaac tccaaaatta aatatcgact tactgaaaga
     6001 attgaaccaa caaatcgatc ttccatttgt tttacatggc ggttcaggta atccggacgc
     6061 agaagtcagc gaagcagtaa aatacggagt tgccaaagtg aatttgagct cagatcttaa
     6121 aagcgtcttt ttcaatgctt tgagagaaat attgagtgaa aatccatcga tgtacgagcc
     6181 gaatgcaatt tatccagcag ctaataaaaa ggtacaagaa gtggtcaaac ataaaatgga
     6241 aattttgaac acagtaggca aggctagttt atactaaaaa taaaaatgtg tggtcaagag
     6301 ggtatgacaa tagtcttgtc ataccctctt aaccacacac gatgttcaga agtagttttt
     6361 tcgaaaaatt tcttcttatt tctcgaagcc aatcacttct gtcacaacct cttttttcct
     6421 gttactatat aaagtaaatg gggtgatttt atgttgaatg aagtggagaa tagacagcag
     6481 gagattgtga attatctaaa aaacaaccaa tttgcaagag tcaccgattt gatcgagtta
     6541 gtaaattata gcgaagccac gagtgaacta ctcaccactt agctaaacct gacggttctt
     6601 aacgcttgaa gtgggagctt cttgggaata gagcatactt gatagcgtat ttctttacta
     6661 acagcggtgt ctcttattta ccaagctatc cccgtagttc ctacggttct tgatgattgg
     6721 atctaagcca atcccataaa aaggcaatac gccttagttg tcggtcttcg agttaccaac
     6781 ggtaaccctc ataccgaacg gcattcatca cccacctata gaggatgggc gacttctgcc
     6841 taattctgtt aaacgtgacc ttgtttttct ggaaaaaagt ggattgatcc gtagagttcg
     6901 cggtggcgcc atgcttattg acaatcgaaa aattgacgtg ccttacctga tgaagatcac
     6961 caaattagaa gaagaaacag aaaaacgtaa gattgcagat acagcttctt ctttgattcg
     7021 agatgatatg gtcttattct tggattccag tacaacctct ttacacttag tcagaaacct
     7081 agcaagattt gatggtttgc agatcattac gaatggcatc gtcacagctg ctatgttgag
     7141 tgaatataca acggcaaaag tatctgtatt gggaggggca attgttccga aacgagcaac
     7201 aatcaacggt tcaaaagcct ataacgatgc attgtcatat tatgcggatt tagcgataat
     7261 gagttgtcgc gggctagatt tcagccaagg agtaacagaa actcatgaag gagaggcaat
     7321 ggtcaaaaaa gcttttagaa aacaatcaga ccggcttgcc gttttagtta cacagcaaaa
     7381 aattgcacat aaattcatgc atcaagccat tgcttttcat gaaatcgatt atctggtgac
     7441 aagtaaagaa ctgacaaaag aacagtatgc gaaagtaaag gaacaccata tcgaatgtct
     7501 gtacggataa attattacgt gaatgttcta agtggcttcc tcatttttag ataggacctt
     7561 atctttgctc gcttctctca gccgatggta taataatgaa aaggttatct tgttagataa
     7621 tcttactttt ttatccagga gaaaggagtg cagaaaatga aacatgtgaa ggcgttggta
     7681 gtgaaggcaa tcatgatctg ggcgattctg tgggtcgtgc tgacaggttt atacggtgtt
     7741 agctttatgg attcaacgat tgttggggta attattgtag tcatgatcta tgtgctaggg
     7801 gatctgttga tcttacgtaa agtagggaac atcgcagcga cgatcgcaga tgctggttca
     7861 gctgcagtta tcttgtggct ctatctatat tttatgaatg atacagtcga tatttggatg
     7921 aaagtattga ttcctgctct attgatcggt gtggcagaat ggttcttcca caagtggttg
     7981 ctaagtcaag gcatcgtacc agatgaacgg aaaatggaat aaatcaacag ttaaactatt
     8041 cttatcgata aatgaaagct ttaaaaataa atagatgttt aattaagaat aatcataaac
     8101 tatttttact caaaaaaccg ctcagattat tttcagatct gaacggtttt ttttatctat
     8161 tcatcaagat caaatttcat ttttcaatga ttcaatcaat tcgccctctg cttcatgact
     8221 atgttttcgg ccattaacaa gatagagtgc tgtacatatc aatggaatga ctccggcaat
     8281 cacatacaat cctctaaatg aagtaagaga ttttagcatc cccatcaaat aaggaccaac
     8341 gcctaagcct aggtctaaac caataaagta ggtagataaa gcaatactga ttcgatgttc
     8401 gcggacgatt tttaaacaaa cagcctgtcc atttgacata aacgtaccat aacctaggcc
     8461 gactaatgct ccagagataa gcaatgtcac actattcgtc gttacactca gtaaaaataa
     8521 tcctgctgtt aagaataaat agctaggata catcacgtat tgttctcctt tgacatcgaa
     8581 aatccttcca gtcaatggac gtgtaaatgt gatgaccaaa gcatatacga cgaagaaaaa
     8641 tgacccagca gcaactaagt ggattacttt tgtataagaa gataaaaatg ataaaacact
     8701 tgaataagaa agacccatca aaaaggcaat tcctgtaatg aaaagtgctt tcttttcaat
     8761 aaaactattg atattccatg agcgaagaga tattttttgt tcttcagata atggaatatt
     8821 ttttacagga aagacaaggc aggcaatcgt aacaaagaag accagtacaa ttgaaaacca
     8881 gataatgaaa cgaaaatcag tcatattcag caaaatcatt cctaaaaatg gcccaacagc
     8941 ggcagctaaa ctagtactta atccataata attgatgcct tccccttttt tagatgcagg
     9001 gatatatgca gtaacgatag catttgtagc tgtagaaact gtaccgtagc cgaatccatt
     9061 caagaatcga actaaataca atgccccgat agttggcgta taaagatatg ctaatgttga
     9121 aaggagatag aagatcgcgc cgcctcttaa ggtaaacttc cgaccaaata gttctaactg
     9181 tttccccatg aataagcgtg ccagcaatgt tccgatgata tagatcccag aagcaaaacc
     9241 tgcttggctc aagctggcgt gtaaggtgtc ttgcgcaatg actgccatga tgaccatcaa
     9301 cagataataa atcaggtaga caataaaatt gatcaatgtg attccaataa aatatttatt
     9361 aaataatttc ttttccatgt atgttataca ttccccttaa gctgaatatg cttttaattt
     9421 ttgtatttga acttctacat cgtgataatt ggatggatag cgatcaacaa gttggcgaat
     9481 aaataaatct cttgtagaca taaacagatt taaaaattct tcgcattcgt ttgttggatt
     9541 ctcatccact gctttgatca attcgatcga gttttgatac gcatcattta aaaagactaa
     9601 agaagctttc atatcaaatc ttgtttgata tgctgtttct aatcgattaa tgattgtgtt
     9661 catccagtaa ttcatgtttt ttccctctat tcttaaaaaa ttttacttct gcagtataac
     9721 gaataaaaaa aaggaaaaaa atcgcataag tgatggaaaa aggtacttaa caaatgttaa
     9781 tatcattagt aaaatacctt tggactttaa aaagttttaa agtactgtat gatcttttta
     9841 gatgaaagtt tggatggggg agcatcattg gatagtagag tcttaaagga atatttggat
     9901 aaaaatgatt ttccgattat acgcaaagga tatcataaat atttgacatt cgaagggatg
     9961 gaagattccc atacgtatat cttgaaagag ggaacggtaa aagcaagtgt tatttcaaat
    10021 gatgggagag aatttaacct tcgctatatc aaagatcttg aaatcgtctc tcttttaaaa
    10081 gacgaatatt cacaattcat cgattcgccg tataatattc gagttgaatc agaatatgca
    10141 gagttttatc gaatcaatcg tgtgcgcttt tggcaaaata tcaaccaaga cccaaagcta
    10201 caacactatg taaaagagta ttaccgctat cgtctgcttt attcgatgaa aaaaatgcag
    10261 caaatgctat taaacggaaa gctgggagct gtttgtacgc aactttacga attatatgat
    10321 ttattcggtg tgcggacaga agaagggatg ctgatcgatt tttcgatcac gaatgaagaa
    10381 atcgccaaat tttgtggcat ctcaactgct agcagtgtaa acagaatttt aaaacaactg
    10441 aaaaaagatg gtattatcac cgttaaagac catcatatcg ttattttgaa tatggacttg
    10501 ttagaagaca atattatctt ttgatgaaca aagaggttgt gaaatcagcg ttttgacctg
    10561 agtattagag aaacaaacgg aaaaatagtt cctcatattt tgccgttttt tgagctaata
    10621 ccgagggtca gctgattgaa caccgtttac caagaggttg tgagcctgcc gtttaactct
    10681 gagtcacaag gaacaatttc aaaaaacagc ttttcatgtt tgatgaatat tgtgacttgt
    10741 gaccgaggag ttggtaggag aacaccgttt accaagaggt tgtgaaaaag ctgttctgca
    10801 tcaagtaata agaagagcca agtaaaaaca gtctcttatg tttttaactt ggctggactt
    10861 atcaccgtag atgtaagctt ttgaacgccg tttatttgca acaaaagggg ctgtgacacg
    10921 atttttgtca cagccctttt tttgcgaaca ttcatttagc aatttttaga aacttctgcc
    10981 gccaccgcca aaagtcccgc caccgctaga atgggtagta cttccgccac cgccgcttcc
    11041 aggaggtggg cttttgggga ttcttcttgt cgtgacaaaa gaatttgtca gacgatcggt
    11101 tttttcagtt aatttgatac tggatttttc tcggaatgga tatttgtaga tgcctgattt
    11161 caattgataa cgcgaaacag tcacaaaata aaagacaaga ctaaggataa gagccaaaac
    11221 aacagcaatg atgatttcta ctgtcgtcaa aaccttatag cgtgtaattt tgccagtttc
    11281 ttcgtctaca cgataatgtc ctcctggaac tccgtcttcc acatatgttg ctgatttaga
    11341 caggtaagct tttgcagcat tgtaataggc agcatttgtc atctgatcat aaacatcatc
    11401 taagatcgaa tcgattctgc tatcagtcaa ataatcgatc atattaccag aagtcgaaat
    11461 gtagatttca cgctgtccca tatctaatag caatactgag ccattttgat catttccgac
    11521 tgcgtctctt aagtaattat cagcaaagtc tcttggttct tctgaattag atgtagtcgt
    11581 tacgatcaag actcgtcctt tgattttttc attcagcttt tctgcctgtt gttccaaaga
    11641 ttggatttga tctgttgtga ataacccagc ctcatcattt accgtagctg tatctgcatg
    11701 agtagccacc ggccagccaa cacttactag caaaagaaag aataaaaaaa attttttcat
    11761 catatcaagt aacctccgat cagaaagagg atcgctagcc cagcgaaaat cccaagagcg
    11821 gtcagtccca attttttctg gctgatcggc aggacaccgc tgacctttcc tgtttgtcca
    11881 ttcattgcgt agtagtagac ttttttactt gagtcattgc ttcgataagt aacaagccat
    11941 accggcagta atacgtaatt atttacttcg tttgtgattg aagcagaagt tcgcacaccg
    12001 gtcaatgttg tatatccatt tgcggtatct tttaacagct tctctgaata gtcttttaat
    12061 tcagattgaa tctgtgattt gattgcctca tattcgatat ctcttttttc tgcttggaac
    12121 ccggctaaat actggctttt aaaatcaatt gctttatcta acgggaaagg ttgcacgcct
    12181 tcgaccatct tttgttgcgt attttttgat aaggcatttt ttactagttc tttaaaagaa
    12241 agcttgcctg ctcttgaaac agcaaattgc ttcgtttctg tgtattcgat gtcacctatc
    12301 cgccatatgc ggataactgt accattcgcc tgcatggcac catctacttc agcatctgta
    12361 gcccaataag ggaaatacac acctgtcaac ttatcgattt gatccttatt gaaaaaagct
    12421 ttgggaacaa accatttttt cttggtccaa gcgagaaatc tgttgattgc ttcttctttc
    12481 tcgacagcaa aaggaagtac tttatttggc aagaattctc cgcttaaacg tcccgatagt
    12541 acgacgggat tatggcagta gtaacaataa gtcgcagcag ttgtcgcatc agtcacgatc
    12601 tctgcaccac agttgggaca aagaaaaaga tctatcgctg cggtattatc tgcagattcg
    12661 tccgctgtat ctgaaaaacc gcctgcctcg tcaaccgctt tgcgctcggc ttcattcata
    12721 tcggcaagct gttcttgggc tgtaaaagta aaatcttcgg taggtgaagg ttcttctttg
    12781 ctttcttcgc cagccatacg cgcttctttt tgcttttgct catattggct gacttcttct
    12841 tctgtataga tattcaggca gtattcgcaa tgaaattttt ggtctttggg atcaaaagtc
    12901 aacggaccgc cgcaattagg acatttatgt gtaagaacat ccatcagctg cacacctttc
    12961 taaataaatt aattaacggg gattccttta aacggtttac cacagtttgg acagaattta
    13021 gggatgccgt tggaaagatc cacgatctca ttgcattcac tgcatcgcat ttgtagctta
    13081 ggctgaacgt tttctacagg tttggcagct ccgcagttgg aacagaattt tccggaattt
    13141 tctgtcccgc aaactggaca tgtccaagta tccgcatttc cttgtgcagt ttgttgattt
    13201 tgtttttctg cttgttgttt gatttgttct tggttatttt gagctgcttg agaaagataa
    13261 gaaccatttg cattcattcc catacctaca cccataaagc cggtcattgc tcctgcttca
    13321 ttttttccgg ctgcttccat accacgtgca atggaacctt gcacgtatcc ttcacgtacg
    13381 ctaggatcac ctagcatcgc cccttcgtta cgcatattga tcagtttcac agaatcatcg
    13441 gtgtaagaaa tgctggctac agccacagaa acaatctcca tcccacgtaa ttcacgccaa
    13501 gaatcatcca atgcagtatc catgtatttg cttaattcca aactcttaga tggtacataa
    13561 gaaatccgtt gaccatctgc agacatctga ttgattgctg attgtagcgc tgttaggaat
    13621 tcagctaaat actgctcatt gatatcattg atttccactt gtgttttgtt tttagggatt
    13681 gcgtttgtat aaaataaaat cgggtcaacg acatgaatag aataagtgcc atgtgcccgt
    13741 aagaataatt ctgcgttata gaaattgtca aagtagttca acggcgcgct tgtaccgaat
    13801 ttgattccct ttatttcttg gagattgata taaaaaactt gttgtttctg cggtgtgacg
    13861 ccgccaaatt tgaatcggtc aaaagtttca gcaattgctt ctttcagtgt tccattgaac
    13921 atggaaggag ctgcgtcatt tttaatggtg taataacctt cctcagcggt ataatcgatg
    13981 atttttccac catcgaccaa aagcatcatc atgtttggat agacatggac gactgttccg
    14041 tctgttaaaa catcttctgt tcctttacga ttggagccac gtttgtcatc tttgcggact
    14101 ttgacacctt ttgtcatgac cgttgtatca ctcatattgt ctggttcgat gacttctagc
    14161 cattgatctg ccagaccgcc gccaaccata cttgttgctg ctttgataag acccataaaa
    14221 aattcctcct ctgattcagc tagtgccaaa caattattgt ttatttatta taccatagga
    14281 agatgtgaaa cttctcggac tagagacgga attgaaatgg aaaattgcgt attgtttaaa
    14341 tacctgaaaa agccgagaag gatagtagtt tttttaccta atatcacgta taataagaga
    14401 tagtgaattt tttaaaaaag ggggatcctt atggcatatc aagcacttta tcgagtctgg
    14461 cgttcgcagc gtttcgaaga catcgttgga caaaaagctg tgacacagac actaaaaaat
    14521 gctattgttt cccacaaaac ctcacatgcc tacttgttta caggacccag aggaaccgga
    14581 aagacgagtg cagcaaagat tttcgcaaaa gcaatcaact gtccgaacag taaagacggt
    14641 gagccatgca acgaatgcga aatgtgtcga tcaattactg ctggtacgca agaagatgtg
    14701 atcgaaatcg atgctgccag caataacgga gtcgaagaaa tccgatttat ccgtgatcgg
    14761 gcaaattatg cgcctacaaa ggcagcgtac aaagtgtaca ttattgatga ggtacatatg
    14821 ctttcaacag gagctttcaa tgccttgttg aaaacattgg aagagccaaa agaaagtgtc
    14881 atattcatct tagcaacgac cgaacctcac aaaataccag ctacgatcat ttcacggact
    14941 cagcgatttg attttaaacg gatcaatacg gcagatattg tcgaacatct tgaatttatc
    15001 ttgaaagaat cgactatcag ctatgaacca gctgcattga atgtgattgc ccgttctgca
    15061 gaaggcggga tgcgggatgc attgagtatt cttgatcagg caatctcgtt tagtgaagga
    15121 acaattactt tagatgatgc catgcaagtg acaggtagct taactgacga aatgatggac
    15181 agttacttga gtgcttgcgt gaaaaaagaa gtaccagaag cattggaaac attgaaccaa
    15241 attctcgcag ccggcaaaga atcacgtcgc tttctagagg atatgttaca atactgccgt
    15301 gacttgctga tgtatcagca agcacctaac ctagttgagg aacaatcgag tacgatcaga
    15361 agtacattta cagaattggc acaaactgtt tcagcggatc agctgttttc aatgataaag
    15421 attttgagcg atactcaaaa tgaaattcgt tttaccaata gtgccgctat ttatttggaa
    15481 gtcgctacag tcaaacttgc acagctaccg tctgcttctg ttccttcagc agatgtagca
    15541 tttgcagatg atcagatagt aaaacagcta caacaagaga ttaaggaact aagaaacgaa
    15601 atcaaggaac tacgcagtaa tacaggaatt gagaatacaa aagtacagcc ggtacagcaa
    15661 acaaatcata aaaaacagac aagtacctat cgtgtaccga cggaacgagt atataaagtt
    15721 ctgcgggaag cgacaagaca gcatctgaac caagtaaaag aagtatggga agacttgctg
    15781 atgagtctat ctgtcactca gcgagcaatg cttaaagcaa gcgaaccagt tgcagctgga
    15841 cctacaggac tagtgattgc gtttgattat gagattgttt gtcaaagagc agcaaatgac
    15901 gaagaattgc agctttcggt acacaacaac ttaagccgaa tgatcaaaga ttatgcgcct
    15961 gatcctgttt atattacaag agaaagctgg ccagaacttc gaagaaatta tttgacgcag
    16021 gcacaagaag agggaacaga aaactctttt gcttctgaag actcagaagg agaaatcgag
    16081 ttgcttcctc aagaacaaga agtaccagaa gaaacacttg tagtaaaagc agaggaactg
    16141 tttggctccg aagctgttac agtggtagat gattaataaa aaggagtaga taattatgat
    16201 gcgtggaatg ggcaatatgc aaggcatgat gaaacaagta caaaaaatgc aaaaagaaat
    16261 ggcagaagca caagagctgc taaataaaac agagtttaca ggtaaagcca ccaatgaatt
    16321 ggtcaaagcg gtttttacag gcgatcgtcg catgaaagat atccaaatca atgaagaagt
    16381 cgttgatcct gatgatatcg agatgcttca ggatcttgtc gtaatggcag tcaatgatgc
    16441 gatcgcacaa attgatgtgg aaactgagaa aaaattaggg aaatacgcaa aaggtttgcc
    16501 aggattttag ttttaaactg aaggagtaat gaatatgcaa tatccagaac caattgcgaa
    16561 actgatcgac agctacatga aacttccagg aatcggtcag aaaacagcga caagactcgc
    16621 cttttatacg atcgatatga aagatgaagt agtaaatgaa tttgctaaat ctttgttaag
    16681 tgtaaaacgc gatttgcatt tttgtagtat ctgcggaaac atcacagaag aagatccctg
    16741 cgaaatatgt agaaatcctt cacgtgatcg aagcgttatc ctagtggtcg aagagccgaa
    16801 agatgtaatg gcaatggaaa aaatgcgaga atatcacgga ctttatcatg ttctccatgg
    16861 tgtgttatca ccgatggaag ggacaggtcc agaagacatc aatatttctt ctctgttgca
    16921 gcggctgcat gatgcagatg taaaagaagt gattatcgca acaaatgcaa caacagaggg
    16981 agaagcaaca gctatgtatc tctcacgtct gatcaaaccc gcaggtatca aagtgactcg
    17041 tctagcacat ggtctgtctg taggctctga tatcgagtat gcagatgaag ttactttatt
    17101 gaaagctgtc gaaggaagac gtgagttata aatttctaat taagtgtttt ttcagataca
    17161 atataaaagc agcacaagag atgggggcaa caatgtgaac ggactattta ttacaatcga
    17221 ggggcctgat ggtgcaggaa aaaccagcat cttgaatgaa ctttaccctc ttttgaaaga
    17281 agtagcaaaa acagaaatca ttcaaacaag agaaccagga ggcattccga ttgctgaaga
    17341 gatccgagca gtgatccttg atccaaaaaa tgatcggatg gatgaacgta cggaagcact
    17401 gctttatgca gcagcaagaa gacaacatct agtcgaaaag gttctgcctg ctctggcaaa
    17461 agggaaaatc gttttatgcg atagatttgt cgacagttct cttgcttatc aaggtgcagg
    17521 ccgacgcatc ggcgttaccg agattgctcg gttgaatgag tttgctacag aaggtactac
    17581 gccggatttt accctttatc ttgacgtaga ttcagacacc ggattaagaa gaatcaagaa
    17641 aaacagacaa aatcaaatcg accgtttaga ttcggaaggt ttggaattcc accagcgtgt
    17701 acgtcatgct tacttaaagc tggcagaaga gaatccggaa agaatccaca aagttgatgc
    17761 aagaaaaagc tttgaagaag tgctgcaaac aagttatcat acaattattg aacagtatcc
    17821 gcaatttttt gaaaactagg aaggtgaacg tttatgaaaa ttattttagc gattatccaa
    17881 gacaaagaca gcaaccgttt atcaaatgaa ttgattgatg caaatatccg tgccacaaaa
    17941 ctatcatcga caggcggctt ccttaaggct ggaaacagta cgtttatcgt gggtattgaa
    18001 gatgaccgtg tagaagaagc attggaaatc atcaaaaaaa cgtgtgaatc aagaaagcaa
    18061 tttgtatcta cacctgttac actggatatt tcgatggatg ggggcgtccc ttatccagta
    18121 gaagtagaag taggcggtgc cacagtcttc gtcttacctg ttgaaggatt ccatcaattt
    18181 taggaggagc tgagctatga atcaagaaat aaacttggct caaatgcaac cgatcgtgta
    18241 tcagcagctt caacggagtt ttgagcatgg gcgtatcgct catgcttatc tttttgaagg
    18301 ggagaaagga acaggaaaac atgaagtagg tatatggctt gcacagcacc tgttctgtac
    18361 ccaaatgaaa gacaatctgc cttgtggaaa atgcaacaat tgtcagcgga tcgaaaagaa
    18421 agaacatcca gatgttttgg ttattgaacc ggaaggacaa accatcaaag tcgatcagat
    18481 cagacgtttg cagacggaat tcagtcgcag cggttacgaa tcaagaaaaa aagttttttt
    18541 gatcaaagaa gctgaaaaaa tgaatagcag tgcggcaaac agtttgttga aattcttgga
    18601 agagccccct ggagattttt tagcggtgtt agaaacagat gcagtaggaa gaattctccc
    18661 tactattcaa tctcgttgtc aagttctgca ttttcaagaa ctttcgaaag aagcactgat
    18721 ccatcgattg caagaagagc agattccatt ggaaaaagca aaacttttgg ctttcttaac
    18781 aaacagttta ggaaaagcag ttgaaatctc tcaagatgaa tggtttaatg atgctaagga
    18841 cttgatttat caatggttcg tttacttgca aaagcaagat actcaagcct ttatctatgt
    18901 ccaaaaaaaa ttggtaaaat catttaaaga aaaaacacaa cagtttacag gattatctgt
    18961 cttgatgttt tattatcagg aagcacagcg tcaagcagta gcaaacggcg aatatggcaa
    19021 agtgaaacag ataaaccaga taattgaacg gatattgatc gcagagcaga aactacgcag
    19081 caatgtcagt tttcaggctg ttgcggaaca gttcgttttg catacgattt tttagcttgc
    19141 cgcaaaggat gatgatcaag gaggatggaa atggtagaag tagtaggtgt ccgatataga
    19201 gatgcaggtc atatctatta ttttgcacca ggagactctg aatatgttta tagtgacaag
    19261 gtattagtcg aatctcagca atccttacat attgctactg ttgtccttcc taaaaaagag
    19321 attgatccca atgaccttcc tgaagctatc cgtccgatct tgcataaagc gtcacaagct
    19381 gaattagaaa aagacaaaaa aaacaaaaaa gatgcttatg atgcattgaa aatcgcaaaa
    19441 gaaaaagtgc agttgcgtga gttggaaatg aaaatggtca aagccgaata cacatttgat
    19501 cgaaataaat tgattttttc tttcacagcg gacgggcgaa ttgactttag agaattggta
    19561 aaagatctgg ccgctgtatt tcgtactcgt attgagcttc gacaaattgg tgtccgcgat
    19621 gaagcaaaga taataggcgg aattggtcca tgtgggcgtc cattatgctg ttcttctttt
    19681 ttaggcgatt tcgtgcctgt atcaatcaaa atggcaaaag atcaaagttt atctttgaat
    19741 ccaacaaaaa tttccggttt atgcggacgc ttgatgtgct gtctgcaata tgagagcgaa
    19801 gaatacgaga cagcgaaaaa agaactccct gactttggaa aagtaattgc tacacctgat
    19861 ggtaaaggga aagtcgtagg gttgaatctg cttagccgta tcgttagtat tcggctgact
    19921 ggacgggaaa atcctgtcga atatgaatgg gatgaattga aagaatttat ccaagtaggt
    19981 gagatgaatg gataagagat cattatacga cggattgaat caattagaat cagagttgag
    20041 aaatacatta acagaacttg ttgaaatgaa gcatgctttg catgagatcg tagaaaaaaa
    20101 tacaactttg gagatggaaa atcaacgtct gcgggaacac ctgcgggaat tgaaccaagt
    20161 tgcacaagct ccagctgaaa atgtgaagca ggaactttct aagtctcgta tgaatcttga
    20221 aaaaatttat gaagaaggat tccatgtctg ctacgattta tatggatcaa gaagggacaa
    20281 tgacgagcct tgtgcgtttt gtctagaagt gatctatcgc gaaagcggtc gatgaccaat
    20341 aacaagagga gactggagcg gagaaatact gctccagtct ttttattaag gagattgtga
    20401 cagatgtata atcaaaaaag tttcaaggga aatccggtat atggggattt atatcttgta
    20461 ccgacgccaa ttggaaattt agaagatatg agctacagaa gtgttcgtat gctgcaagaa
    20521 tccgatctta tcgcaagtga ggatacgcgt aatacacaaa agttattgaa tcattttgaa
    20581 atccatacac cccaaaaaag tttacatgaa cataattata aagaacgtgt ccctcaattg
    20641 attgctttgt taaaagaagg aaaaacgatt gctcaagtca gtgacgcagg aatgccttcc
    20701 atcagcgatc ctggacatga gctagtttta gcctgcataa aagaagggat ttctgtgatc
    20761 tctatcccag gacctacagc tgggatgacg gctttgattg cttcagggct agtgcctcaa
    20821 ccatttttat tttatggctt ccttccacgt aagaaaaaag aacagaaaga aacattgaaa
    20881 aagctaaaag aacagacagt tacgctgatt ttttatgagt caccttatcg gatcactgct
    20941 acattgacga atttcttaga aatttttgga aatcgccaag tcgttttgtg tcgggaattg
    21001 acaaaggtcc atgaagaata ccttagagga agtacggaag agctactcgc ttatatcgaa
    21061 gaacatccag taaagggtga atgttgtctg cttgtagaag ggaatttgtt cgaaatgcca
    21121 gtgacggaag ttgatgaaga gttgggaacg ttaaaggaac aagtagaagc aaagatcgct
    21181 tcaggcgaaa aaccaaatgc tgctatcaaa gcagtagcgc tgaaaaatgg gctgaaaaaa
    21241 caagaagtct accgtcagta ccatgaatta gactgagata gtataagttg tctataatga
    21301 aagactaatg aggaaatcag ctactaactt ttcaaatctc ttgaacgcga acgaatgttc
    21361 ttgtataatg aataagaggt gaggatgatg gagctgcaat ttcctttaca taatgataca
    21421 tcaagaaaaa tcattcatat tgatatggat gcttttttcg cttcggttga agaacgagat
    21481 catcctgagt ttcgcggaca tccattagta attgccagac accccagtga cactggtggg
    21541 cgtggagtag tgactacagc aaattatgaa gcaagaaaat tcggaattca ttcagcaatg
    21601 agtgcacaaa aagcgtatga gctttgtccg caagccatct ttaagcctgg cgaccatcat
    21661 aaatacagcg atatttccaa acaagtacgg gaagtattct atcgttatac agatattgtg
    21721 gaaccagtta gtatcgatga agcgtatctt gatgtgaccg aaaataaaat caattgcaag
    21781 tcagccatca aaatcgcccg aatgatccaa gcagatattt gggagagtgt ccagctaaca
    21841 tgttctgcgg gagtaagtta caataagttt ttggccaaat tagcatcaga ctatcaaaaa
    21901 cctagaggaa tcacggtagt tgcccctcaa gacgctgtgc catttttaaa agctttgcca
    21961 attgaaaaat ttcatggggt agggaagaaa actgtaccta gaatgcatga gctagggatt
    22021 tatacaggaa aagacctata tgaatgcaca gaaatgatgt tgattcgaaa ttttggaaaa
    22081 atgggctatt ctctttatcg taaagtcaga ggaatccatg attctccagt aaacgttaca
    22141 agagagcgaa aatctgtggg aaaagagcat acttatgggc agccgttgca aacggaagaa
    22201 gcagtgctga ctcagttgcg ccaactagct gaaaaagtag aagaagcact gagaagagtc
    22261 caaaaacatg ggaaaacagt agttttgaag gtcaggtaca cggactattc tactgtgacc
    22321 aaaagagtga cattgccaga atatatctat aaaaaagaag cactctttta tcaagcaagc
    22381 cttatatggg aggaaatctt gggagtagaa aaaggaatcc gattgttagg gattactttg
    22441 acgaatctgg acccgatgac ttatgaaaac atcgtccttc cattatggga aaatcaagaa
    22501 atataaaaac agctttgaga gttgaaaccg gaaatccgtt ttctctccca aagctgtttt
    22561 tatatcaagt cttgcttttt gatttgttca taggtttctg ttgcatccgt acatttttcc
    22621 caaatcacaa cttgattgga ttctaaagat ttttggacgt caatgatccg ctggttgcta
    22681 cttccgcgaa attgtagttt taggtcacgt ttgcttaatt caaagcgtcc gtcaacaagg
    22741 atatcgattt gcgaaaggag ttctaattta tcttctgaat cttgtagcaa ttcatcaaag
    22801 gtatagcctg tccatgacca gatatctttt gtcgtaccga actcttttcg gatacgtcgt
    22861 acgacttgca gacaaacatc tgtattcaaa aaaggttcgc caccaagtaa agttaatcct
    22921 tgcacgtaat cgttgcgcaa gtcagcgatg atttgttctt ctaattcagg tgtgaatggt
    22981 ttgccatatc ggaaattttg tgcagctttg ttgaagcagc cttcgcaggc aaataagcag
    23041 ccactaacgt aaagactgtt gcggacacct tcaccatcga caaaattaaa cggtttgtag
    23101 tcagcaatat agttttggct gtagtctttt gctaaccatt cttttggttt tggatttctc
    23161 ataaaaaaac ttctttcatt taaggttcaa agatatagct attgaagata aaatctactc
    23221 gaccacataa ataagaacaa actgacctaa atccattgtt caagaaagct tgttcttatt
    23281 ttgatagagg gcgttcaaag atacaaagcc atcgaaagag ggtggtgaaa aaagacttgt
    23341 catagcctct tattctgaat aaacggtgtt caaaagcttg catctgcagt aataagccca
    23401 actaaacgaa aaatatgagg aactattttt gtttagctat tcttattact tgatgcagga
    23461 cagctttttc acaacctcat ttcatgtgtt tcacacgtga ggaaatttct ttatggcggc
    23521 catgtaccat cggacgagct tgcggatttc ctaaatagcc acaagtacgt ttgactacat
    23581 cacatgtttt tggatcatga tttccacatt ctggacattc gaaccctcgt tctgtcggtt
    23641 tgaaatcacc ttcgaaacca cattgatagc aatgatcgat tggcgtattc gtaccaagat
    23701 acccgatttt gtcatatgca aaatcccata cagcttctaa agctttcgga ttttgttgga
    23761 gcatagggta ttcacagtag tggatgaatc cacctgaaca atattttgga taatccttct
    23821 caaattccaa tttttcaaat ggcgttgggt ttttacgcac atcatagtgg aaactgtttg
    23881 tataatattc tttatctgtg atattttcga tgatgccgaa tttttctgta tccaaacggc
    23941 agaaacggtc agttagactt tcacttggag ttgaatagac actgaaatga taaccatatt
    24001 catcccccca tgcatctgca ttagctttta attctttgac gatatcaagt gtgaaagctt
    24061 ttgcttctgg gtttgtttcc cattcgccac cgaagaaagc tgaagcaact tcatacaatc
    24121 caatgtagcc taacgataca gtagctcgac ggtttttgaa taattcattg acagaatcgg
    24181 tacgtgaaag acgtttcccg aaagcaccat acatatataa aattggcgca tttgctggaa
    24241 tggcttcttt acaacgttct acacgataga gaagcgcatc tttcatgatt tctaagcggt
    24301 cagacagtaa ctgccagaat ttattctgat ctccttttga ttcgatcgcg attcttggca
    24361 aattcaatgt aacaacacct aaattcattc gtccgacatt tacttcttga ccattttcgt
    24421 ctttccaccc ttgcaagaat gaacgacagc ccataggtac tttgaaactt ccagttaatt
    24481 caacgatttt atcgtaattc agaatatcag gatacattct tttcgttgca cattccaatg
    24541 ctagttgttt gatgtcgtaa ttaggatctt cagggtttaa gttcacacct tttttcaaag
    24601 tgaagatcag tttagggaag atagctgtcc gcttttcact ccctaatcca ttgattcgaa
    24661 tctgtaaaat tgctttttga atttctcttt caaaccagtt ttctcctagt ccaaatccta
    24721 atgaagtgaa aggagtttgt ccatttgaag taaataaggt attgatttca tattccaaac
    24781 tttgcatagc atcgaagata tcttttttgg tttttgcttt tgcatattct ttttggcgtt
    24841 ctggcgaatc gatccattct tccgcgtctt ttagatgttt tttatagttc aattctgcaa
    24901 aaggagcaag caattcatct gttcggtcgg cagaacagcc accatattga cttgacgcta
    24961 cattggcaat aatctgtgag atttgtgctg tggcggtctg gatagatttt ggcgattcga
    25021 cttcggcatt gccgattttg aacccattgt tcagcatgcc tttgaaatca atcaagcagc
    25081 aattcgtcat cggtgtataa ggatgataat ccaaatcatg gtaatggata tcgccttttt
    25141 gatgcgcatt tgctatatgt ggtggcaaca ttttcagtcc gattgatttt ccaacgatcc
    25201 cggctgtcag atctcgctgt gtattgaaaa cattactgtc tttatttgcg ttttcgttca
    25261 cgacagtttg gtccttgttg attagctttt caatcgaaaa gttgatatct gttgctttac
    25321 tgcgagcaaa atctcgtttg gtacgataat tgatgtactc ttctgctaat tcatattcgt
    25381 ttttttctag taatatatgt tccacgatgt tttgaatctc atagattttg acattttcag
    25441 caaaccggct tttgatttct tggacaacca agtctgcaat catttgaatt ttttcataag
    25501 taagtggtga taatgagccg tgaattttct tttctgcttt gatcagtgct tcattgatct
    25561 tttgttcatc gaaatcaaca tggcggccat cgcgtttgat gactttcatc tgggatgtct
    25621 ctggctgctg agtaacgaca gttttttctt cataatttac tttcattatc ttcaactcct
    25681 gctaaattgt tcttgtctat cataaaacaa aatcggggaa ggagcaacta aatatcggct
    25741 atatatagta ttcaatgtgc tgtataattg gatttctata ctatatgtag tgtatatttt
    25801 attgtgaaaa aaggtgaaaa cttgtagctg gtggatttgt tttttgcata tttaattttg
    25861 ctgaataaaa taattgaata aaatttttta ttcagtccaa gtaaaagtga caaaaacata
    25921 agagcaaatt attcgtagat atgctactat gcaaataaat agaaatgaaa aatgaaagag
    25981 gaggagaaga attggaaaag atgatcgaag tgaaacatct aagtaaaagc tacggcagca
    26041 aactttctcc cgtcaaagcc ttagataata tctcattcac agtagaagaa ggcgagtttg
    26101 ttgggattat gggcccaagt ggtgcaggaa agacaacgct gatgaatatc ttgtccacga
    26161 tcaatttgcc tacgatgggc agtgtctcga tccaaggaaa agaaatcaca aaaatgaaaa
    26221 acagtgagct cagtgacttt cgtcgaaaaa aactaggatt catttttcaa gagttcaatt
    26281 tgatcgatac cctaaatgca aaagacaata tcctattgcc cctagcagtt gaacggatga
    26341 ctaaagagga aatggaaaag agagtaagac atgtagctca attgttgaat atagaagaac
    26401 tgctcaaacg gtatcccgat gaactttctg tcggacagag acaacgaatt gcagctgcta
    26461 gagcacttgt tgttcagcca caagtgattt ttgcagatga accaactggc tctttggact
    26521 cgaagtcggc tacagaattg ttgaactact tgaagaccat gaatcaaaaa gaaaaagcaa
    26581 cgatactgtt agtcacccat gatccatata cagcaagcta ctgcgataga attttattta
    26641 ttaaagatgg tgtgattttt tctgaagttg tcagaagagg gacaagaaga gagttctttg
    26701 agaaagtaat agacatgcag gcgacgattg gcggaggagg aaaaatgaat gctgtataaa
    26761 ctagccgtta aaagtttttt tcggcagtca cgaggctatc ttgtttattt tttcagtttg
    26821 actttatccg taatgatcta ttattccttc agcgcaatga cctacgatca atctttagtc
    26881 agaagagcaa gtcaagatac ttcgatagat ggtggattga gttttggtgg atttatcatt
    26941 actgttgttt tgcttttttt cgtattcagt gccaaccgat ttttcttgaa tagaaggcaa
    27001 aaggaaatag gcatctatca gctttttggt atgaataaac tgcagatatc aggtatatat
    27061 gtgttagaaa tcatgataat tggattgttc gcctgtattt ctgggatatt actcggtatt
    27121 attttttcca aactcttttc aatgattttg gttcgaatga tggatatgga tcttactagt
    27181 ccatttttta tctccattcc atcagtagta gatactttat ttgctttttt catcatcctt
    27241 ttagcagtat ctgtatattc aatatggaaa atatggcgct atccaatgat tcgaagtttt
    27301 ggagaaaaag aacaagcaga tagttcaaca atgcgcttca ggacaaggca ccgattactc
    27361 gcagtcatcg ggttactact tttaacgagc ggatattttg gagctagtca ttttagggaa
    27421 atcacttcat ggatgatcag ttatcattat cttgatacat tagttttctt ttttcctttt
    27481 ttaatttttt tcgtttgtgt ggtcggtact tatttgtttt tctgttatac atttcggtta
    27541 tttttgatca tattgagtaa atggaagatt tattactatg gtatcaattt tttgataatg
    27601 ggtaacacgc aagttcattt gctcaaaagt tggcgtacaa attctctgat tacagtcatt
    27661 ctcggaattt ctttaatgac aattggcggc acggctagta tctctactat tttatcctac
    27721 caggagaaag tttcctcccc gatggactat cagtttgatg ttgagacaga gaaaaaaatc
    27781 aagccgattc tagcagagga agggcagaag ataactaaag aaatcacctt gcattataaa
    27841 gtcattggga gtatctatag tcaaaatctc gaagaaccta tgtttcaaac tgctaatctg
    27901 atcacggaaa cacaatatca agccttcaga aaagttaatc cagatttgcc gaacatttct
    27961 ttgaaatcac ctggtcatac tgttttgctt gatcaaatgc aaactctttt tcggaatatt
    28021 tcattatatg gatctagtat tggactgcca gataatcaag aattgaagat tcaacaaatg
    28081 cttccaagtg tattaggtga cgaaacaatg gtatatctaa gtcctgtact gattgtagat
    28141 gataccgttt ttgaaaaagc aaaaggtgtg gattatcaag tagtcaatgt agatgtatca
    28201 ggtggaaata atgaaaaaat agatgaacga ctgagcaatg acctgtcgat aaagtggctc
    28261 aatgctgttt attattctta tgatatcgta aatggaagtc tacaaggaga aattagcaca
    28321 aagaaacttc cggatgacca aatgaaggaa gcacaattta gccaagaaag ctctcgtttg
    28381 aatttctctt ccagctattc gaaattgcgt gcagttaatc ggcgaatcgg tatcaatatt
    28441 tttgtcacac tatttgtcgg gatgatcgtg attattgcta ctggtagtat tttgacagtc
    28501 cggcaattag cagaagcaga ggaagaaaaa gaaaattatc acctattgac taagctgggg
    28561 attccacaaa gaaggatcag acggatgatc ttcgagcaaa acgcacttac cttctttcca
    28621 cccatgatat tagggatcac tcacgcagta tttgcaatca atgtcttcac tcagtatata
    28681 aaaactgccg attactggtt ggcttatctt gtgagtggct tattgatcct tatctatctt
    28741 ttgctttatg tcattacgag tcagttatat tgtcggatta tcgaggaatg atatacggaa
    28801 taagacaaga taataaat
//
LOCUS       DAIGOK010000023        28519 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.023, whole genome shotgun sequence.
ACCESSION   DAIGOK010000023 DAIGOK010000000
VERSION     DAIGOK010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.023"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            123..1409
                     /gene="efmA"
                     /locus_tag="NOA01_000791"
     CDS             123..1409
                     /gene="efmA"
                     /locus_tag="NOA01_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HCI0632009.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(1547..2146)
                     /locus_tag="NOA01_000792"
     CDS             complement(1547..2146)
                     /locus_tag="NOA01_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HCI0632010.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            2550..3671
                     /gene="mnmA"
                     /locus_tag="NOA01_000793"
     CDS             2550..3671
                     /gene="mnmA"
                     /locus_tag="NOA01_000793"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCI0632011.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            3917..5338
                     /gene="arcD"
                     /locus_tag="NOA01_000794"
     CDS             3917..5338
                     /gene="arcD"
                     /locus_tag="NOA01_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HCI0632012.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            5352..6470
                     /locus_tag="NOA01_000795"
     CDS             5352..6470
                     /locus_tag="NOA01_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HCI0632013.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(6530..7822)
                     /locus_tag="NOA01_000796"
     CDS             complement(6530..7822)
                     /locus_tag="NOA01_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCI0632014.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            7954..8532
                     /locus_tag="NOA01_000797"
     CDS             7954..8532
                     /locus_tag="NOA01_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCI0632015.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            8661..9038
                     /locus_tag="NOA01_000798"
     CDS             8661..9038
                     /locus_tag="NOA01_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HCI0632016.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            8971..11043
                     /locus_tag="NOA01_000799"
     CDS             8971..11043
                     /locus_tag="NOA01_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCI0632017.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(11254..12201)
                     /locus_tag="NOA01_000800"
     CDS             complement(11254..12201)
                     /locus_tag="NOA01_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCI0632018.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            12447..12911
                     /locus_tag="NOA01_000801"
     CDS             12447..12911
                     /locus_tag="NOA01_000801"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCI0632019.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(12973..14016)
                     /locus_tag="NOA01_000802"
     CDS             complement(12973..14016)
                     /locus_tag="NOA01_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HCI0632020.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(14393..15358)
                     /locus_tag="NOA01_000803"
     CDS             complement(14393..15358)
                     /locus_tag="NOA01_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HCI0632021.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            15592..16437
                     /locus_tag="NOA01_000804"
     CDS             15592..16437
                     /locus_tag="NOA01_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCI0632022.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            16628..17815
                     /locus_tag="NOA01_000805"
     CDS             16628..17815
                     /locus_tag="NOA01_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HCI0632023.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            17821..18177
                     /locus_tag="NOA01_000806"
     CDS             17821..18177
                     /locus_tag="NOA01_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HCI0632024.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            18179..18517
                     /locus_tag="NOA01_000807"
     CDS             18179..18517
                     /locus_tag="NOA01_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HCI0632025.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    18593..18849
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            18912..19946
                     /locus_tag="NOA01_000808"
     CDS             18912..19946
                     /locus_tag="NOA01_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCI0632026.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            19939..20625
                     /locus_tag="NOA01_000809"
     CDS             19939..20625
                     /locus_tag="NOA01_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI0632027.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            20649..21497
                     /locus_tag="NOA01_000810"
     CDS             20649..21497
                     /locus_tag="NOA01_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCI0632028.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            21694..22467
                     /gene="sufC"
                     /locus_tag="NOA01_000811"
     CDS             21694..22467
                     /gene="sufC"
                     /locus_tag="NOA01_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCI0632029.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            22480..23766
                     /gene="sufD"
                     /locus_tag="NOA01_000812"
     CDS             22480..23766
                     /gene="sufD"
                     /locus_tag="NOA01_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCI0632030.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            23763..24998
                     /locus_tag="NOA01_000813"
     CDS             23763..24998
                     /locus_tag="NOA01_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCI0632031.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            24985..25455
                     /locus_tag="NOA01_000814"
     CDS             24985..25455
                     /locus_tag="NOA01_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HCI0632032.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            25460..26851
                     /gene="sufB"
                     /locus_tag="NOA01_000815"
     CDS             25460..26851
                     /gene="sufB"
                     /locus_tag="NOA01_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCI0632033.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(26949..28091)
                     /locus_tag="NOA01_000816"
     CDS             complement(26949..28091)
                     /locus_tag="NOA01_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCI0632034.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENYSVKMYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYPQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
     gene            complement(28142..>28519)
                     /locus_tag="NOA01_000817"
     CDS             complement(28142..>28519)
                     /locus_tag="NOA01_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCI0632035.1"
                     /translation="NEILANNINPLIEEYANQRHSISKELFFKRANSKLQDKIRNGDF
                     KYGEKERMIDSLKQLLDEMDLAYKDISKYSSVEDFFYKITKNQFETEKFISDLSKKYN
                     FPYEKNSIIAKFLTDNHDKFKNN"
BASE COUNT         9255 a         5236 c         6058 g         7970 t
ORIGIN      
        1 gtaaagaaat ttaaattgaa acgattgtta tttagggaaa aaggtatttt ttgaaaaaag
       61 aatcccgtat aatgattgtt gtgcaaacgc tatacaaaat gaaaaagaaa agagggatgt
      121 ttatggaaaa tgaacagtcg gtcgttttaa cgaattggaa gagaaattat ttgttctttt
      181 tatcgggaca gtttttgtca ggaatcacta gtatggtcgt ccaatatgcg atcatctggt
      241 atttgacaag agaaaccggt tcggcgacaa ttttgagttt tgctacttta ctaggaatga
      301 ttcccatggt tttactaagc ccatttgtag gaccgctagt ggatcgctgg gataaaaaag
      361 ctcttttgat tgtcacggat attattgtag cgattttcgc gcttatttta gcagtagtcg
      421 gaacgatttc agaatccttc ccgatttggc tagtttttgt gtctttgttt atgcgttcgg
      481 tcgcacaaac attccaaatg ccaacgatcc agtcgattat gccgacaatc gttccatctt
      541 ctcacataac aaggacgaat ggacagttag gaatggtcca atcagcgaat ttcatcattg
      601 cgccagctct aggtgcggct ttgttttcag tcgttcctgt caattacctt attttattag
      661 atgtattggg ggcagttttt ggtgtcggct tattgatttt tgtgaagatc ccaaaagttt
      721 ctcctgaaat attggaagtt ccgcttacta tttttaaaga tgcaaaattt gggctgcagc
      781 aattgatgga taataaaggg ttatggtaca taacgatcaa tggtgcattt gtgatgctgt
      841 tgttcatgcc agcaatcagt ctgtatccat tgatgacgct agattacttt ggtggttccg
      901 ttggacaggc gggagcagtg gaagtagtct acgcagtcgg aatgctctta ggcggtgcac
      961 tgatcagttt tataggaaca tggaaagacc gaatgaaacc tattattata gcctacatca
     1021 ttatgggcct gacgatcggt gcaagcggat tggttccaaa cgatagtcaa gggttcttgt
     1081 actttcttat cttgaatgca ggagcaggtt gtgcaacgcc atatttcaat acactgctga
     1141 tggcaatgat ccagcagagt tatgaatcga atgttttagg tcgtgtctta gggaacttca
     1201 actctttgat gaacctggca ggtccaattg gattattatt tgcaggacca ttagctgatc
     1261 gtttaggggt agaaaaaatg ttcttgtttt ccggaatcgg gattttatta tgcggaatcg
     1321 ttttattttt aacgtcagct gcccgaaatt atgataaaga attacagaaa aaactggtaa
     1381 aagaacacca tgagcaaaaa gacgaatagt atggaatgga gttgtgacag aagtcttgtt
     1441 acaactccat ttctgatcct aatgttcctg tggcaataag cctactcaaa tgaaaaatat
     1501 gagtaattat ctttatttgt ttaatacttg atatagggca gttttttcac gacctatcgt
     1561 acttcctctt tgcttcatag atttctttga tttgttgctc agataacgga gtgccaaatg
     1621 gtagtttttc ctttaagagt tctctttccg gtgcgtctac gcctacattg acatcgttct
     1681 cgataggtac gctgtaatga tggatatgtc catgaagatt gcggatagtg tcgttaggcc
     1741 cgagcaacat cggataatga gtcaagtaat attgttgatg gtcgaattta acgatggcac
     1801 ctacatcata aaaataaaat ttgggacttc cgtcagtaag cctaggatca tttttttcta
     1861 aataacggaa aaaagcgcga ctgtcatggt tccctttgat caagtggatt gttccttgca
     1921 gttgagtgac cagttccaaa atttctgcat ttgctttttc atattgcgga tgcatagcaa
     1981 ggtcgcctag atgataaaca tggtcggttt cttttattac tgcattccag ctagtgatca
     2041 actgttggtt catttcatcc acatttgcaa agagccgcgg agcaaagtcg ttttttccta
     2101 ataagcgttc atggaaaaag tgcatatcac tgataaagta attcatgtat cttttgcctc
     2161 ccttttttct tagagtaaac aaaatcactt atctgagcaa ctcataacat ctaaaaagaa
     2221 aaggttgtaa atgagacgtg cagctttaag cgataagaag caattttgaa aaatagtttt
     2281 tcacaattta taaaattatt ccttattgcc gaaaggctgg ccctttttat gagtatgcca
     2341 attttttata tcgaatgctc aaaagatgag tgattttttg gttgactatc atgtttgtgt
     2401 actgaatagg tccagtagaa cagtattttt acgacttgat aattaaggga tgaaattttc
     2461 agaaatatga cgtataatgt aaaatactga aaagttgcga ccttcaactc gtagatgttt
     2521 cagaatctaa ttgaaatgat ggtgatatca tgacagacaa cagcaaaaca cgcgttgtcg
     2581 tagggatgag cggcggtgtc gactcatctg taacagcact tttgttaaag gaacaaggat
     2641 acgacgtgat cggaatattc atgaaaaatt gggatgacac ggacgaaaac ggtgtatgca
     2701 cggccacgga agattataag gatgtagcga aagtagctgc tcaaatcggg attccttatt
     2761 actctgtgaa ttttgaaaaa gaatattggg atcgtgtatt tgaatatttc ctagccgaat
     2821 accgcgcagg acgtacacct aacccagacg tgatgtgcaa caaagaaatc aaattcaaag
     2881 cattcttgga ttatgcaatg gatcttggcg ccgattatgt agctactgga cattatgcgc
     2941 aagtcactcg cgatgaaaac ggcgtggtcc acatgcttcg tggagtagac aataataaag
     3001 atcaaactta cttccttagc caattatcac aagaacagct ttcaaaaacg atgttccctc
     3061 taggcggcat ggaaaaatca gaagtacggg caattgctga acgcgcaggc cttgcgactg
     3121 ctaagaaaaa agattcaacg ggaatctgct ttattggtga aaagaacttc aaacaatttt
     3181 tgagcaacta tctgccagca aaaaaaggaa atatggtcac tcttgatggt gaagtcaaag
     3241 gacaacatgc tggcttgatg tattatacga ttggtcaacg tcaaggacta gggatcggtg
     3301 gcggaggaga ttcgcaagaa ccctggtttg ttgtaggaaa agatctagcg acaaacacat
     3361 tatatgtggg gcaagggttc catcatcctg cattgtatgc cacaagcttg gatgcaagtg
     3421 aaatccactt tacgacgaat gaaccaatgc caaaagaatt caagtgtaca gcgaaattcc
     3481 gttatcgtca gcaagacgtt cctgtgacag ttcgcttatt ggatgataat cgtgcagaag
     3541 tcgtattcga cgaacctgta cgtgcaatca ctcctggaca agcagttgta ttttatgatg
     3601 gaatggaatg tctgggtggt ggtttgatcg accacgcata tcaagaaaca aaagttttac
     3661 aatacgttta agcgatatta aatgaatcaa aacagctatg gctaacaaaa gaaacacctt
     3721 ttcttttgga tagcatagct gtttttttgc aataaaatta atttttattc attttatact
     3781 tgaaatactt gtgtaaaaag ttcataatat ccaataatat gaataaagtt gctttttgtt
     3841 aagcaagagc agttgcaaaa tagaagggaa tcttttgcaa gtgatttgtt caattttata
     3901 agtgagggtg atggaaatgg aaaagaaatc gacgggtatt agcaaatggg gactggttgc
     3961 tctggttgtc agttcgtcaa tcggaagcgg tgtgtttggg atcacaagtg atctggctag
     4021 ttctgcagct ccagggccag cgatcctttc atggataatc gttgggattg gtattttagc
     4081 tttggtatta tcgctgaatc atttaggaga aaagcgtcca gatctagatg gtggtatttt
     4141 cggttacgcg gaagcttcat ttggtaaatt aggtggcttc atcagtggtt ggggttactg
     4201 gttgtctgcg tggctgggaa atgttgcttt tgcaacgatg ctgatgagcg ccatgggaga
     4261 atttttcccg atattcaaag gtggacaaaa tgttccttct attttatttg caagtatttt
     4321 tatttggggt ctgactttat tagtaaacaa tggagttgaa ggagcgagcg tcatcaatac
     4381 aatcgtgacg atctgtaaat tagtgccatt attcctattt ttaatatttg tatttatcgc
     4441 atttaaagtg aatatattca ctgctgattt ttggggtaac gtatctgata atctagtgaa
     4501 tggaacaggg cagcaaggaa cgatctggtt gcagattaaa ggttgtttga tggttatgat
     4561 gtgggtcttt gtagggatcg aaggcgcaag tgttttagcc aaccgcgcga agaaacgttc
     4621 agaagcccag caagcatcga tcattggtct gctttgttta ttgtttatct atatccttgc
     4681 ttcgatcttg ccatacggcg tcttatctca agaagagtta gcaactattt cacaacctgc
     4741 tatggcaaat attttaaaag gaatcgtggg aaattgggga gcagcgctga tcaatatcgg
     4801 cttgattatt tcgatcattg gttcttggtt gtcttggacg atgctcccag cagaaacaac
     4861 gatgttgatg gctcgagacg gtctgttgcc gaaaggttgg ggaaaaacga ataaaaaaaa
     4921 tgccccaact tattcgttag ttgttactgc aatcttgact aatctattct tattgacttt
     4981 cttagttact gattatgcct atcaatttgc ctattcttta tgtacagcgg caattttgat
     5041 ttgttatcta cttgtcggga tctatcaagt ccagttttct ttaaaagaag gaatcaaaaa
     5101 acaagtagtg atcggtggga ttgctgtagt ttttgaattg atgggcatca ttttagcagg
     5161 attttcttat gtccttttat gtagtattgc ctatcttcct ggtttttgct tctactggat
     5221 cgcttgtcgt gaaaccaaac atcaaattac gggaaaagaa aaagtaatga ttgggctgat
     5281 ttgtgtggcc gcactgcttt caatcgggtt gcttgctgta ggctggatca agatataagg
     5341 gggaaagaaa catgaaaatc gcaaatttcg gtgtagaaga atggctaaat aaatgggaaa
     5401 agaaagcggt atatgatatt gcacaaagct cgatcgaagc attgacatta gacgaactcg
     5461 ttggcttaga tgggactagc gtaagtaaat ttttcgaaaa acgcaaaaac gaaccattgg
     5521 actatggctg gatcgaagga tcagatgttt tcaaacaaga agtcgcttct ctttatcaaa
     5581 ccgtcgatcc ggaaaacatc ttacaaacga atggagcgac tggtgctaat cttttagctc
     5641 tatacgcgtt agtggaagca ggagaccatg ttgtttcgat gcttcctact tatcagcaat
     5701 tatatgacat ccccaaatct ttaggtgccg ctgttgactt tgttcatttg aaggaagaag
     5761 aagattggca gtttgattta gaacagctag aagccttagt caaacctgaa acaaaaatca
     5821 tctgtttgaa tagtgcaaac aatcctacag gcacattgct agatcggaaa actttagaaa
     5881 aaatcgcaga aattgctcgt acagtagatg catatgtatt gatagatgaa gtatatgcgc
     5941 ctttaacgga taaaggtgaa ttcttgtcga tcgtagatgt ctatgaaaaa ggtatagcaa
     6001 ccaattccct atccaaaacc tactccattc caggtattcg tattggctgg acagctactg
     6061 gacctgagtt ggcagaagtt tttcgaaagt atcgagacta cacgatgatt tgcggcggag
     6121 tcctgtcaga tgatcttgct gtacatgcat tgaagaataa agaaaaaata ttggcgcgaa
     6181 acaaaaagat cattacagag aaccttgtga ttttaaaaca atgggtagcc aatgaaccga
     6241 aagttgaact tgtcgctccc aattacgtct ctacttcttt cattaaatta gccatcgaag
     6301 aagatgacca gacattttgt atcaatttat tagaagaaac aggagtacta ttagtaccag
     6361 gaagtgcctt tgatttaccg aagcatgccc gactaggtta ctgttgtaaa aaagaaacct
     6421 tggaaaaggg attattcctc ttatctgagt tcttgaaaaa acaggcctaa acaaaaacac
     6481 tgcaaggaca gtctgtgaca actgttcaag cagtgttttt catttacgct tattgtctat
     6541 gagaaggaga gtgccctgaa gctatggaga ctcctgaagc tttttcttct gcagtttctt
     6601 gctgatggat ctcttgtgta ttgtgtcgta caagcaagaa gttgaccaaa acaagaagag
     6661 aagtcgaaag aaagacgccg cgataaccaa atgcactgga aacactggag ccgatcatag
     6721 gccctactac gttaccagtt gcttggaaag attggttata actgaagatc cgtccagcag
     6781 cttgatgagg cgaatatttt gtgatcaatg cttggacagc agggagcaga caagcatcag
     6841 agataccaat caaaaaacgt aaggcagcta attgccaaac attcgtgacg aatgccattg
     6901 ggatataaac cagaatcgca aaacccaaac caatcgcaag tattctttca ctcccaatct
     6961 tgtcacctaa gcgaccaaat cttggagcgg ctatcaaagt ggcaatccca ggtatcgaag
     7021 caatgattcc gctgatcaac gtaacattcc catgtccatg cagcaattgg cgaatgtaca
     7081 gactgatgat tggactaatg gaattgttgg atgcttggat gatcatcgtt gtgacgaaca
     7141 taccgatgac gacgtgtgga tatttcaagt ctttgaaaat ctgtttggca gaaaccatgt
     7201 tttctttttt cactgggaca aatttttcat ggacgaaaaa cagactcaac aagaagacga
     7261 aaaataagat gatccctgta atgaaaaagg tagggcgata gccaaacgct gaagcagaga
     7321 tcccgccaag aagcggtcca agtaatgttc ctgtaacaga accagttgct aaggttccaa
     7381 gaacttgacc acttttttct tttggcgtcc cagttgcgac aagggcagtg gcattactga
     7441 tataaccaga aaaaatccct tgaagcagac gcaatgcgac taattgatac acactagtca
     7501 cgcaacccat caaagaaatg acgattgcca ttcctaatga agcacgcaac agcatcaatt
     7561 ttcttccttt ttggtcggct aattttcccc accaaggaga gataagcgta gtcaccaaga
     7621 aagtagatga aaaagtcaat ccgctccaaa agttcaattg tgacgtacta taattaccta
     7681 acgtatctat gtaaagtgac ataaaaggca tgactaaact aaatccgata ccagccataa
     7741 atgttccaaa ccataagaca aacaagtttt gttcccaagt ttctcttttc ctaaataatt
     7801 tttcctttat tcttgcagcc aattagactc cttctttctg aaaattttca tctacagctc
     7861 tttattataa actacaatat gtagtttatc aatgaaagtc tcttttttct ctttcttttt
     7921 cgtggtaaac tgaaagagtg aaggatttat tttatgagag gaaacatgga aatgaaaaaa
     7981 agaaatctgt ctcaagagaa aatcatcgac tgctttcgag aactagcaga agagatggat
     8041 gttcagcaaa taacctttca gcatttagcc aaacgcttgg atatcaaggc tccctcgctg
     8101 tataaccatt tcaaaaatat tcgtgatgtc aaaacagcgc taacagcgaa acttctgcag
     8161 gaacttaatg accagctgcg acgggctctt gtgggaaaaa gtggtcagga agcactgcag
     8221 atctatgcag aaacttatcg atcctttgct ttttcaaatc aagcagtgta tgagttactg
     8281 atcagtgtac cgcataccaa tgaagctgtt ttattagatg gaatccatga aacgaatcag
     8341 atcattttgc agatatttga ggcttttcaa ttaacgaaag aagaaaaagt tcatcgaagc
     8401 agggaacttc gtagtatgat ccacggttat ctttccttac gttttttagg atactttaca
     8461 aaagaaccaa caatcgatcc agaagacagc tatttttgga tgatcaataa ctttatcgct
     8521 actttgcctt aaacaatcag attcatttgt aacaatttta agatgttcat taaatgaccc
     8581 ttttataaga gaaaattctt gcaactttct tgaaaagtga ctaagatgag tctagaaaag
     8641 ctgaaagaag gagcacgagt atgtatcaag taattaccat gttcggggac aatgagcctt
     8701 ggtggttttt tgaagactgg gaagaagata ttgaagaaga agaaacattc gagtctttcg
     8761 aagaagcacg acaagcgtac gaaaaacaat ggaacgaaat ccatcaaaat tatgaatata
     8821 tcaatgcaaa aagtaacttt ctcagcgcct tttggaatga cggagatgag cgctggtgtg
     8881 aagagtgtga cgatgattta cagcaataca agggacttgc gttgttgaaa aatcatcagc
     8941 ccatcacagt agaaagcaga aaggaatttt atgaaacaac taattctagc ggaaaaacca
     9001 agcgttgcga aagacctaag caaggtgctt ggtgctaatc aaaagcacaa aaattattat
     9061 gaaggaccca aggtgatcgt tacttgggcc cttggtcatt tattaggatt gaaaatgccg
     9121 gaagatctga ataaggaatg gcagagctgg caaatggaca cgttgccaat gatccctaaa
     9181 aaactaggaa tcaaaccact tcctaaaaca ggacatcaat taaaagcaat caagcagctg
     9241 gctcagcgaa aagatgtctc agaagcagtc attgcgacag atgccggaag agaaggggaa
     9301 cttgttgctc gctggatctt agagtatgtg catttcaata aaccagtgaa gcggttatgg
     9361 atctcttctc aaacagataa agcaatcaaa actggattca agcaactaag acctgcgaaa
     9421 gaatatgatg cattgtatga atctgctctt gcccgggcaa aagcggactg gctggtagga
     9481 ttgaatgtga ccagagcctt aactgtgaag tacgcggata atttatcagc aggccgtgtg
     9541 cagacaccga cactagcatt agtgagagac caagaaaaga aaatcgagca gttccgtcca
     9601 caaacgtatt tcacgatcac gttgcaagct ggaaaagaac aagccaaaat gcttcaaaaa
     9661 aatcaatttg ctttaaaaac acaagaagaa gcacaaaaac ttgttcaatt gatcagtaca
     9721 agcaaaggaa ctgtcacaga agtccaagaa aaattaaaaa cggaatctgc gccattgcct
     9781 tatgatttaa cagagatcca aagagaagcc aatcaacgct atggctattc tgcaaagaaa
     9841 acattaagtc ttgtacaaag tttgtatgag acccataaga tcgttactta tccgcgtaca
     9901 gacagcaaat atctgacaaa tgacatgaaa agtacgatga aagaacgatt gcaagcagtc
     9961 agcgactttg ctcctgaggt caagcagtat ttgaaaaacg gagccatcgt ccaacagaca
    10021 aaagtattcc aagacagtaa agtgaccgat caccatgcac ttttgccaac tgaaaacaga
    10081 gcacgttatg aaaaattaag taatgaagag cgaaaaatct atcaaatgat tgtctctcgt
    10141 tttttaggac tgtttgctca accccataaa gtcagccaaa caaaagttac ggttgaattt
    10201 ggtaaagaac gattcatttt tcggcaaaat cgtgtcgtcc aagcaggatg gaaaggcgaa
    10261 actgaaactg agacggaaac agtcaaatgg gaaaaaggaa tgagaatcaa tcccgatttc
    10321 gccatcaaaa aagaactatc tgcaccgcct aaaccattgt cagaagcaag cttgcttggt
    10381 caaatggaaa aatacagtct gggaacccca gctacgcgtg cagagattat cgagaaacta
    10441 atcaaatctg aactgatgga acgtacccct caaggactgc aagtatctcc taaaggtaag
    10501 caattattag tattggtaaa tccctcttta gtaacaccag atttgacaga aagctgggaa
    10561 aaagaccttg aagctattgc tgcaggaaag aaacaagcag gaaccttctt gaatggaatc
    10621 gaaaaagaaa caaaacgctt ggtgaatgag atcaaatcaa gcaaacaaga atatcaagat
    10681 ttctcgatta cccaaaagaa atgcccagaa tgtggggcca atctgcgcga gaaaaacaca
    10741 cgtgacggca aaatctatgt ctgcaccaat tcagattgca actaccgcag aagaaaagat
    10801 ccaaaagtct ctaatcatcg atgcccgcaa tgtcaccgga aaatggaaat catcgaaggt
    10861 aaaaacggtg cttatttccg ttgtaaatac gatggaacga cagaaaaatt gcttgataaa
    10921 aaagagcaga agaagaaaat gaccaaacat gaagaacgac gcttgctgaa aaaatatgca
    10981 caagcagaag aaccagaaga aagcccatta gcagcagcac taaaagcagc aatgaagaag
    11041 taataagata tgaaagaggt tgtgatagaa gtggtcagct tcaagaaata agaaggaatt
    11101 tacgaaaatt gcttctcaaa tttttgtgaa atttcggctt atttccgaag aagctacttc
    11161 tggaacacca tttattcgga aaaaaggact gtgacaagac ttttgtcaca gtcctttttc
    11221 atttaaaaaa atgtttactt catttgacgt atattattgg tgaggcttca attcatttgg
    11281 cttcactttt tctgtttctc ttatgatata aacaggacgc tgtttcgttt ctaagaagat
    11341 tttcccaata tatttcccga tgattccaag acataacaat tggattccac caatgaacag
    11401 gaagatcgag accatggaag gccatcctga tgtaggatca ccaaacaaca atgcccgcaa
    11461 aataatgaac aaaattgcaa taccagaacc gacacaagac aaagcgccga taaatgaagc
    11521 aatcgttaac ggtgcatccg agaagttcac gataccatca atcgagtagt tgaataattt
    11581 ccagaaggac caagatgttt cgccagccac acgttcgcgg ttttcataag aaatatattc
    11641 tgttttaaat ccgacccagc taaataatcc ttttgaaaat cggttgtatt ctgttaattc
    11701 taggacggca tccaccattt gacgagtcat caagcggaaa tcacgagcgc catcgaccat
    11761 ttctgtatcg ctgattttat tgatcaactg gtagaatttt ttcgcaaaga aactgcgtat
    11821 cgcaggctcg ccttcacgag tcgaacgccg ggtgccgaca caatcgatat cagaagtagt
    11881 gatccggttg atcatctcag gaagcagttc gggcggatct tgcagatcaa catccatcac
    11941 ggtcactaaa tctcctgtag aagcttgcaa tccagcgtac agagccgctt ctttcccaaa
    12001 atttctggaa aatgagacaa agcggacata aggataagct gaggctagct tcttgattgc
    12061 tgctaacgta ccgtcttttg atccgtcgtc aataaacaaa tattcgaact catgggcacc
    12121 tttgaatttt tcaacctcat caaaaaaaag aggaacagtt ttttcttcat taaaacatgg
    12181 gacaacaatt gatactttca tcgtttctat cctttcaatc gtatgtttat atatggttta
    12241 cattcaaaat ttaataaacc ttaaataaca cctttaaaat ttgattttac gtaaaagaac
    12301 aggtattctt tttgtaaact aacgtattta gtttaccatg tttcctcggt ttatcaaact
    12361 tttaagaaca atttttatta gttggcaagt agttttggat agagaaaaag gaggaagttc
    12421 cagtctattg actaggagca aacaaaatga aaatacacac acctatcggc tttttatggt
    12481 tagatgcaga tgaatccgga ttgactgctg tatcttttca gccaatttca gaaagcgaat
    12541 cagcgaatca gcgaatttta acgcgcacac aagaacagtt tgaagaatat tttgcgggaa
    12601 aaagaaaaga atttgatgtt cctttatcta taaagagagg tacgcctttt cagcaaaatg
    12661 tctggaaagc tttgtcgacc attccttatg gagaaatccg aacgtataaa gaagtagccg
    12721 ttgctgttga tagtccgaaa gctgtgcgag cgattgggca ggcaaatcga accaatccat
    12781 tgccaatcgt tattccttgt catcgagtga tcgggcaaaa tggaaaactg acaggatata
    12841 tgggaaacgc aatagatggg gttgaaatca aacgccaatt gttgttgtta gaaggatatc
    12901 tgcaaaatta aaaacagttg tgattcggac attgttccat gcgagaatca caactgtttt
    12961 ttcatgttca ttctaaactt tgatcagcca aataaatagt aaagccatca ctgtttggag
    13021 aagcccgaca gctaatccat aagataagaa gcgtgtacct tctttcatga attttgcaaa
    13081 gtctaaacgc agaccaattg ccgctaaagc agtgatctca aaccagctgc tgataaaatg
    13141 tgccgattgg tcaataacta caggtaaaga aaacaagctg ttgaccaaac aaaagatcac
    13201 aaaccctaat acataccaag gaagcggtaa ttttcgtttt ttctgcgtat cagttggcgt
    13261 ttcaaacaga gctggttgtt tcttgtgcat gaatttttca aacagataga caacaacaac
    13321 gagcatcata atacgtagaa tcttaaatag catggcgaat tcgacaacag aattgtcaag
    13381 taagcttgcc ccagcgacta cttgaccaac agattggagt gtaccaccca aaagtgcact
    13441 tttttcaatg agatttccac caaacaactg taatccaaga aaagggagag tcaacatcat
    13501 cacagtaccc agtagattga ctagtgtgat gatctgtcct ttttcttcct ctttcgcatg
    13561 gatggaaggg gcgattgaag caatagcaga agaaccacaa acagcgtttc ctccagccat
    13621 catcaaagaa gaagtatcgc tgaattgcag ttttttacca atcaagtaag caccgatgat
    13681 agtgactgac atttgcagta acacaaaaac aattcctttt acaccaattt gagcaatcgt
    13741 ttgagctgtg acagttgcac ctagtaaaaa aacagagaat tctaatagtt tgctttcagc
    13801 aaattttgtg ccgattgcta gaacaggctg tttaaaaaaa gtatttccta ataaaatacc
    13861 caataaaata gcaatagtcg ctccacctaa ctgtggcaga taaatagcaa gaagcctgct
    13921 gaaggcagcg atggcaaaag aaagcagtaa accgggtaag atttcgcgga ccgctgaatc
    13981 ctttacagat tttattggat gtgacaatga tttcatttga tcggttcctt tcgtcaataa
    14041 atactaaaag catactcgaa catagtagca ctaaaccagt attttgacaa aagaacaaga
    14101 ggtcgtgaca aaagtcttgt acgacctctt gtttcgaata aacggtgttc cagaagtagc
    14161 ttctacggaa ataagccgaa atttcacaaa aatttgagaa gcaattttcg taaattcctt
    14221 cttatttctt gaagctgacc acttctttta cgacctcttg gtaaacggtg ttcaaaagct
    14281 tgcatctgcg gtaataagcc caaccagtac aaaaacatga acagctgttt ttgtttggcc
    14341 gttcttatta cttgatgcag aacagctttt tcacaaccta ttgaatcgat tattaagcga
    14401 tttttttaga aacaaatttt ccatgtttgc tatgttcttt aattaagaaa cgtacgtaat
    14461 gagcaagaac gtagaagaca actacacttg caaacagaca ttccgcaact tttaagaaat
    14521 ccaatacgct actatagata cctaaactgt tgaagtaatg aacagtagta aatatagctg
    14581 ttgcagagat aacaagcggg aatgtaaagg ctgcatagct tggataaaaa cgaagcttga
    14641 acaaacgtgg aagcatagac agcacaacga aatacaatac ttgagataaa gcgaacaatg
    14701 ctacgacaag ataaaaatta ggttcagcaa agtttttaag ataacctgtt aggcaaagag
    14761 aaccaggtgc tgcaacgatc gtaatcagcg gacgtgtcgg atgagccata tttcttacga
    14821 aaaagacacg tttgatgatg atcggcaata agaacgcata gcagatcaat gaaatccaaa
    14881 agaaaatttt tccaataagt gggaagaagt tcccggcagt tacagtgatg accccaaatc
    14941 caacgtaaat gataaaccag cttggataaa ttgcttcaat tgaaagatca gctttgataa
    15001 catggtgata attgaaataa aagacaagga tcacttggaa gataacagcg cacagccaaa
    15061 tgaatttgac aattggtgca atactttcat atgaaacaaa ataagtacaa ataaccatga
    15121 gtgacatcgt aaaagttggt gaaacggagg cgatgattgg atcatgcaac gtttgtttcg
    15181 tgtgttcgaa taagataaac aatttaacaa aaatgaatat cataagaatc atagagatac
    15241 cgccagttag attgcctaaa gcaacatggt gatagtcttt aaacagattt ccaagggaag
    15301 ctaacccaag gatcaacccg cagatcggga tcggaataat tttcaaaaag ttcttcatat
    15361 ataatattct cctttttatt taatagattt tagtaccaag atgaacgaat acattgtaaa
    15421 atgaatagga atgtatgatt acgagcaagc agacagctga ttgtcttcat ctaagttact
    15481 agttggatca gcaactgttt tgttgtacat ttctattata gatggttttt tgtatattta
    15541 aatcaaatag tttttatagt aattatcata aaaagttatg gaagtggtgc catgtttaag
    15601 ttattaacga cttttcgagc agtatatgaa acaagaaatt tttccagagc agcagaagta
    15661 ctgtttttat ctcaaccagc agtctcgaat caaatcaaac agttggagcg ggaattgaat
    15721 atcgatcttt ttcaacgtaa cggaagaaaa gagatgcttc caactaagca agccgactta
    15781 ttatatgaac ggacactggt tttgttagaa gaatgggaag aaactcagca aaaattgctc
    15841 aatgaacaaa atgaagaaga agtatgtcgg atcgttgctt cccacacctt tgctgtctat
    15901 cttttgcctc agttgatgaa acatctgatc aaaaggtttc ctaaagtgca ttttgagatc
    15961 gaaatcacga attctcatga atcattagaa caagtagcga aacatgaatc cgattttggt
    16021 tttatcgaaa agccattaga gacttccggc gtacaaagat actctttgat ggaagaccag
    16081 ctagtcatcg ctggggaccc aaaaagcaaa ttgtggcttg taagagaagc aaactctggt
    16141 gtttttcatt acacgcagcg ttattttgaa gagatgaata tccaagataa gaagatgatc
    16201 gtgaaaagta acgatgtaat tgttgcgttg ctgaaggaag gaatcggtcg ctcgattttg
    16261 tctaagcgct ctgttccaga aaatgtgcca atgactccgt tgaatttcca tcgatatttt
    16321 tatttcatcc aacgaaccaa tctggtttct gatcagctgc tagagattgc cgaagagatc
    16381 cttcgataca atgaaagcac agaaacttct cacagcgagg agaatctcag ctggtagata
    16441 attgcatgta tcaaaaaaag ttctatgata cactaaagat aagcagtagg cttttgtact
    16501 gcatttgctc aagcaactgc acaaacggaa agttaatttt tcccaaatgt ctatggaaag
    16561 actttccatt atctaactgg accgctataa taatcagcag tatggttttt aagaaaaggg
    16621 atgaaacatg gaaaaaaaat taaaagcttc gaacgataat cgtattgatt atggcgtgat
    16681 tttaccagtc tttctgttat gtttgattgg tctgctctca ttatatgtag cacttagcca
    16741 tgatccgaac aatccagcag tcttgaaagg cgtagggatg caagttgtct ggtatttagt
    16801 tggagcggca gcaatcgttg tcatcatgca tttgaattcc aaatggatct ggaaactgac
    16861 tccttacctt tatggtttgg ggttagtggt aatgggactc ttgctcaaat tctatgatca
    16921 gaatttagct gcttcaacag ggtctaaaaa ctggtttcga ttcggtgcgt ttactttcca
    16981 gccagcagag ttgatgaaga ttgcgtacat cttgatgatg gcactgatcg taactaaaca
    17041 taatacgcaa gtaaaagaac ggacgatgaa atcggacttt tggctgattg ggaagctctt
    17101 gatcgtgaca ataccggttt tagcattgat catggcccaa gatgactttg gaacgatgct
    17161 tgtctttctt gcgatctttg gcgggatttt cctgatgtct ggaatctctt ggcgcatcat
    17221 cgcacctgta gtaatcttgg cagtagtcgt tggagcagga acgatcttct tggttacgac
    17281 tgaaggcgga agagacttgc tttataaagt tggattcaaa tcttatcagt ttgcacgtat
    17341 tgattcatgg ctggaccctt tccatgatac gtcaggaatg agttatcagc cggcccaagg
    17401 acttttggca attggtaccg gtggcttgtt tggtaaagga ttcaacgtaa gcaatatcta
    17461 tgtgcctgtt cgtgagtccg atatgatttt taccgttatc ggtgaaaact tcggctttat
    17521 cggtggtgca ttcgtcattt tcttgtattt tattttaatc tatcgtatga ttcgggtgtg
    17581 tttcgatacg aataatgaat tttacgccta tatcgcaagc ggtttgatca tgatgctgct
    17641 gttccatgtc tttgagaata ttggagcaaa tatcggatta cttcctttaa cagggatccc
    17701 attgcctttc attagtcagg gaggatcctc gattttaggg aacatgatcg gtatcgggtt
    17761 gatattatcg atgagatacc aaaatgaagc acctgcaaga tcttcaagga ggtaagaaaa
    17821 ttgtacacct tttactggta cccgaaatgt tcaacatgca aaaaagccaa agcgtggtta
    17881 gaccagcatg gcgttagtta tcaaaccgtt gatatgatca aagaagtacc gaaggcctca
    17941 acgattgaga cttggctaga aacgagtcaa gtaccaatcc gtcgcctgtt caacacaagc
    18001 gggatgaaat atcgggaact tggactgaaa gatcaagtag gcgagtttac ggcttcaaaa
    18061 gcgagccaag tcttatcgac agatggtatg ctgatcaagc gaccactgat cttacgagag
    18121 gaccagcttg ttgcaattgg attcaatgaa aaaatttatg aaggagaact gatataaaat
    18181 ggcaggcaag catttgaaga aaaaggacaa cctttggatc ttatttaatg gaaaagaata
    18241 ctgtgttggt ttgacaaatg aagcgcaaga agaacttgga gacatcacgt ttgccaatct
    18301 gccaaaagca ggacaatcgc taaaaaaaga tgaacctctg atcgaagttg aagcggaaaa
    18361 agcggtcaat gaatttgcca gcccgctaac gggtacagtt tcttctgtta atgataaaat
    18421 cggtgaagat atcaatatct tgaatgattc agatgaaatg aatgcgtgga tcttaagttt
    18481 caaagatgtt gatccaaaag aatttgacga attataaaat cagaaaatca cttgaaaaaa
    18541 gttgacactg attcttccag ttgctataat cataagcaat cctatatgaa acgagaaagc
    18601 tttgaaaaga agagtacatg tcacggacgt ttgaagagag ccctgtttgc tgaaaatggg
    18661 taacgaaaat gagatggaag atggtcttag agcagagtaa gcgagcgtaa gcaaacttac
    18721 tacgggagtg cccgttacag cgcgacagta tacggaaaga agctaagtcc aagtactgga
    18781 agaggctgtt attgcgaagt aacggtaaat caaggtggta acacggaagt tcaagctttc
    18841 gtccttttgc ttaaggcaag agggcgaagg cttttttgtt tgaaaaatta taagagaaag
    18901 atgtgaatgg catgtcatta atccaactaa cgaatgtcaa aaagacattc aatgggaaaa
    18961 acggaaacat ccaagccgtc aatgacgttt cactaaacgt tgaaaaaggg gatatttatg
    19021 gaatcgtcgg ctattctggc gcaggaaaaa gtacattagt ccgtctcctg aatggacttg
    19081 agcttcccac gagcggcgaa gtgatcgtaa acgatcaaga tattacaaaa ttaaaaaaca
    19141 aagaattacg tacattccga aagaaaatcg gtatgatctt ccagcatttc aacctgttat
    19201 ggtcaagaac tgtacttgaa aatatccagc tgccgctgga attagctggc gtaccaaaga
    19261 gcaaacgtaa agaacgggca gaagaactgt tgcgtttagt cgggttagaa ggacgcggac
    19321 aagcatatcc ttcacaactt tccggcggac agaaacaacg cgtcggtatc gcacgagcat
    19381 tggcaaatga tcctgagatc ctgctttgtg atgaggctac tagcgcattg gacccgcaaa
    19441 caacagaaga agtattagac ttattgttag cgatcaataa gaaattgaat ttgaccatcg
    19501 tcttgatcac acatgaaatg aatgtgatcc gcaaaatctg caacaaagtc gctgtgatgg
    19561 agttggggaa agtcgtagaa gaaggagacg tattgactgt cttcagacaa ccgaaaaaag
    19621 aagtaaccaa acgctttgta caacaagaca tcgaaccaga agctgattcg acagaactat
    19681 tagctgaact catcaaagaa aatccaagcg gccgtttggc aactttgacc tttagcgaaa
    19741 gaaatgccaa cgagccagtc atttcccaag cgattcgacg ctataacatc gatgtcaacg
    19801 ttgtgtatgg aaagatcaaa caagtgaaag aaggttcctt tggttcattg actgtcttga
    19861 tgactggtga aaccaaagaa ttagatcaag cagaagtgtt catcaaagaa caaggagtag
    19921 gaattgaggt gatccatcgt ggataaaagc ttttcagaaa catatctaga ttttaaacaa
    19981 atcaacccgg aaacgatgaa tcaggctatc cttgatacac tatatatgac attaatctca
    20041 atggtcttag tgacgatcat cggattagtt ttgggattga tcctttactc tattggacgc
    20101 aaaaacaaac cgtatgctcg aatcatttat ggaatcgtat cgatcatcag taatattttc
    20161 cggtcaacgc catttatgat cctgatggtt ttgatcattc catttactaa agcaatcgta
    20221 ggcactatgc taggtgcgca agcagccatt ccagctcttg ttttatccgc agcaccattt
    20281 tatgcccgtc tagtagagat tgcttttcgt gaagtcagcc ctggtgtact ggaagcagca
    20341 gatgcaatgg gagcaagtta ctgggaaatc atctggaagg tcttgatacc tgaaagtatt
    20401 cccgcattga tttctggatt aacagttaca acgatctcga tgatcggatt tactgcgatg
    20461 gccggcgcga tcggcgcagg tggacttgga ggtcttgctt ggcaggaagg ctaccagcgt
    20521 ggtaatctaa cagtgacatt cgttgcgact ttaattattt tactaatcgt atttatcgtt
    20581 caaggaatcg gtgatttctt aactaagaaa acagacaaaa gataatagga tataggggga
    20641 tgtaggatat gaaaaagaaa atttttggat ttgcagcagc gcttttgctg actgtaggat
    20701 tagcagcatg cggtaacaac agcgacagca aagattcagc aaataaagca gacgacacga
    20761 cattgaaagt cggcgcttca ccgacaccgc acgcagagat tttggaacat gtgaaaccat
    20821 tattgaaaga tgaaggaatc gatcttgaaa tcgtcaaatt cgatgactat gtattaccga
    20881 accaatcttt ggaagaaggc gacattgatg ccaattactt ccagcatatt ccttatttga
    20941 acaaagaaat caaagaaaaa ggatatgact ttgtcaatgc cggtgctgtc cacatcgaac
    21001 caatgggctt gtactctaaa aaagtcaaag atgtttccga attaaaagat ggtgcaacaa
    21061 tcattacttc aaattctgaa tcagactggg gacgcatcat cacaattcta caagatgctg
    21121 gcttagtcaa agtcaaagat ggagtagatc tggaaacagc aacattcgat gatattgaag
    21181 aaaaccctaa gaacttgaag ttcaaccata cgatcgaccc atcactttta gcttctacgt
    21241 accaaaatga tgaaggtgac ctagttgcaa tcaacgcaaa cttcgcttac ggcgcagggt
    21301 tgaatccatt agaagatgcc gtattgcttg aaaaggacaa ctctccttac gtgaatatta
    21361 ttgcgactcg taaaggtgat gaagacagtg acaaaatcaa aaaactgatt gaagtgcttc
    21421 accgcaaaga agtccaagaa tggatcacag acaaatgggg cggctctgtt aaaccagtaa
    21481 aggctgatgc aaaataaggt atggaacaaa aaagatgaga aataagtctg taatggctta
    21541 tttctcatct tttttatacc tattctaatt tagaaatcaa aattgtttaa taataaataa
    21601 aagatatttg attttagtaa ggaaggtcat tgcgcttcat tctcaattag tctaaaatga
    21661 gtgagaacga aagagaattt gggggtataa tgcatgtctg tcttagaaat aaaaaatcta
    21721 catgtgtcga tcgaggaaaa agaaatcctt aaaggcgtaa acctaacaat gaaaaccgga
    21781 gaaatccatg ccatcatggg acctaacggc acaggaaaat ctaccttatc tgccgctatc
    21841 atgggcaatc caaactatga agtaacagaa ggcgaaattt tgtttgacgg gaaaaatgtt
    21901 ttagacttgg aagtagatga acgtgcgcga cttggcttat ttttagctat gcaatatcca
    21961 agtgagatac caggtatcac gaatgcggaa ttcatgcgtg cagcgatcaa tgcgaaacgt
    22021 gatgaaaacg acaaaatttc tgttatggca tttttgaaaa agttagatga aaaaatggct
    22081 ttactaaata tgccagaaga aatggctgaa cgttatctga acgaaggttt ttctggcgga
    22141 gaaaagaaac ggaatgagat cctgcaattg ttgatgttag aacctacatt tgcaatttta
    22201 gacgaaatcg attcaggtct ggatatcgat gctttgaaag tcgttgctaa aggagtaaat
    22261 gaaatgcgcg gagacaattt cggtgcattg atcatcaccc actatcaacg attattgaac
    22321 tatatcacac ctgatgtcgt tcacatcatg atggaaggcc gagtagtcat gacagggaat
    22381 gctgacctag ctaaacgtct ggaagcagaa ggctacgctg gtatcagtaa agaattagga
    22441 atcgactata aagaagaaga agcgtaaggg ggagcaagaa tgaaagaaaa aaactggctc
    22501 gattatcttg acgcggtaaa tgatttttct ttaagtaaag gcgaacctga ttggatgaga
    22561 accttccgtc aagacgcact agcgaaagca gatgaactgc cattgccgca tatcgatcgg
    22621 gtgaaattcc atcgctggtc gttatttgat gtgaaagaaa cacaaaccat cagtgaaaca
    22681 ggcacgattc cggcatttga tgcaatgaaa gacaatcctg tgcttgtcca acaaggttca
    22741 tggacgattt ttgaacaact gcctgtggaa ttagcagaaa aaggagtcat ttttaccgac
    22801 ttgttcactg caatgatcga atatccagaa ctagtccaag aatattatat gaaaaaagca
    22861 gtaaacatga acgaagacca attgactgca ctccatgtag cttttatgaa cagcggtatt
    22921 ttcctgtatg tgcctaaaaa tgtcgtgatc gatgaaccac tcgagtcctt attcattcaa
    22981 gatggcgcat ccgatgagca cttcttcaag catgtgttga tcgtggcaga tgaacatagt
    23041 gaattttctt atctggaacg tttccagaca accaaagaac aagtagcaaa atcatcaggt
    23101 aatatcatcg tggaagtcat cgctaaagct ggttcaaaaa tcaaatattc agccgtagat
    23161 caattaggag aaaatattac ttcttatatg aatcgccgtg ggcacatttt acgagatgct
    23221 tctgtcgact gggcaattgg tgtcatgaat gacggtcatg tgatcgctga ctttgactca
    23281 gatttagcag gagaaggtgc acacgcagaa gtcaaaatcg tggcaatcag ctcgggcaga
    23341 caaatccaag gaatcgatac acgagtaacc aataaagccc ctcatacgat cgggcatatt
    23401 ttgcaacacg gagtgatccg agaaaaagga acattgacct ttaatggtat cggccacatc
    23461 ttgaaagggg caaaaggtgc agatgcccaa caagaaagtc gtgtcttgat gctttctgat
    23521 aaagcacgtg gtgatgcgaa tcctattctt ttgatcgatg aaaacgaagt aactgctgga
    23581 catgctgcca gtgtaggacg agttgatcca gaagaaatgt actatctgat gagtagagga
    23641 ttgcataaag aagaagcaga gcgcttagtg attcgaggat tcctcggttc tgtcttaaca
    23701 gcaatccctg tagaacaagt tcggaaagaa ttagtcgaag taatcgaagg gaagttgaat
    23761 ggatgatcga tgctaaaaag ctacgcaatg attttcctat cttatttcaa gacgtcaatg
    23821 atgaaccttt agtttatctg gataatgcag cgacgaccca aaagccaaaa caagtactcc
    23881 aggcattagc gtactattac gaacacgata acgccaatgt tcatcgcggt gtccatacgt
    23941 tagcagaacg cgcgacccat gattatgaac aagcaagaga aaaagtacgg gcgttcatcc
    24001 atgcaaaaga aacagcagaa gtgctgttta ctcgtggtac gacgacaagc ttgaattggg
    24061 tagccagaac cttcggcgac cgctatatca aagcaggaga cgagatagtg ctctcttaca
    24121 tggaacatca ttctaatgtt atcccgtggc agcagttggc aaaacgaaca ggtgctgtct
    24181 tgaaatatat cgaactaaca gaagaaggct tcctggatat ggaggatgca gcgaaaaaga
    24241 ttacggacaa aaccaaaatc gtctccattg ctcatgtatc caatgtctta ggtgtcttca
    24301 atcctgttca agaattagct gaacttgtcc atgaaaacgg cggggtcatc gtagtagatg
    24361 gtgctcaatc tgccccgcac atggccattg atgtccaagc actagactgt gatttctttg
    24421 ctttcagcgg acacaaaatg tgcggaccaa caggtattgg tgttttatat ggtaaacgaa
    24481 aatggttaga agaaatggaa cctgttgaat ttggtggcga aatgatcgat tttgtcgaat
    24541 tatacgacag tacatggaaa gaactacctt ggaaattcga agcagggaca ccaaatatcg
    24601 ccggcgctat cgcattaggc catgcaatcg attatttgca aaaactcggt atggacaata
    24661 tccatcgata tgaagaagaa ctagccgctt atgtgttgcc aaaattacaa gcaattgatg
    24721 gattgactgt ttatggtcca caagacccga attttcggac aggagtcgtt gcattcaatt
    24781 tggatgggtt acatccccat gatgtcgcga cagctttaga catggaaggg gtagccgtca
    24841 gagcaggtca tcattgtgcg caaccgctat tgaagtacct tgaagtatca gcgactgctc
    24901 gcgcgagctt ttatttctac aacacaacgc aagacgccga ccgtttagtt gaagcaatca
    24961 tggcaacaaa ggagtttttc caacatggga ctatctaaat tagacaattt ataccgtcaa
    25021 gtgatcttgg atcattcttc ccatccgcat catcgtggaa gcttgactga ctcgacaagc
    25081 aagatcgaat tgaacaaccc cacatgtgga gatgtgatcg aattgcaagt cgatgtaaaa
    25141 gacggaatca tcaaaaatat cgcctttgac ggcagtggct gctccatcag tacggcaagt
    25201 gcctcgatga tgacagacgc cgtcatcgga aaatcaatag aagaagcaga acatttaact
    25261 gtagactttc tacagcttgt ccaaggaaaa gaagtcaaag atcccgacca attaggcgat
    25321 gctgctatgc ttggtggtgt agctaaattt cccgctcgga tcaaatgtgc gacacttgcc
    25381 tggaaagcac taggaaaagc aatggaaaac gatggtaccg cagctttcca tcattaccac
    25441 aaggaagagg agtgagaaca tgggcgtacc agaattagaa gaatataaat tcgggtttca
    25501 cgatgatgtc gaaccggttt tcagtacagg agaaggcttg acagaagaag tgatccgtga
    25561 gatgtcaaga atcaaaggcg aaccagaatg gatgcttgaa ttccgtctga aatctctaga
    25621 aacatttaac aaaatgccaa tgcaaacatg gggccccgac ctttcagaca tcgatttcga
    25681 tgccatcaaa tattaccaaa aaccaagtga caaacccgct cgtgactggg aagacgtacc
    25741 agaaaaaatc aaagatacat ttgaaaagat tggtatacca gaagctgaac gagcttatct
    25801 agccggtgct tccgcacaat atgaatcaga agtcgtgtac cataatatga aagatgaatt
    25861 cgaaaagtta ggaatcgtct ttactgacac tgactctgct ttgaaagaat accctgaatt
    25921 attcaaagag tacttttcaa aacttgtacc gccaaccgac aacaaattag cagcgctgaa
    25981 ttcagccgta tggtcaggtg ggacctttat ttacgtgcca aaaggcgtca aagtcgatgt
    26041 tccattacaa acgtacttcc ggatcaatgc ggaaaacacg gggcaattcg aacgtacgct
    26101 gatcattgtt gacgaaggag caagcatcca ctatgtcgaa ggttgtacgg cacctactta
    26161 ttcaagtaac agcctgcatg ccgccatcgt agaaatcttt acaagaaaag atgcgtattg
    26221 ccgttacaca acgatccaaa actggtcaga caatgtgtac aacttagtaa ccaaacgagc
    26281 aaaagcatac gaaggcgcaa ccgttgaatg gatcgacgga aacctaggtg caaaaacaac
    26341 gatgaaatac ccaagtgttt acttagatgg aaaaggtgca cgaggcacca tgctctccat
    26401 cgcctttgca ggagcaaacc aaatccaaga tacgggtgca aaaatgatcc acaatgcacc
    26461 aaatacatct agctctatcg tctctaaatc aatttcaaaa gacggcggag aagtaaacta
    26521 tcgaggacaa gtaacctttg ccaaaaacag tgcgggttcg atctctcata tcgaatgtga
    26581 cacgatcatc atggacgaat ggtcgaaatc cgatacgatc ccattcaatg agatccacaa
    26641 cagccaagtc tctttagaac acgaagccaa agtctcaaaa atctcagaag aacaactcta
    26701 ctatctgatg agccgaggcc tgaccgaatc tgaagcaact gaaatgatcg tcatgggctt
    26761 tgttgaacca ttcaccaaag aacttccaat ggaatatgca gtcgaactca atcgattgat
    26821 cagttatgag atggaagggt ctgtgggtta acaactattt cattgctcga aacgttgata
    26881 ttaaagcgtt tcgagcgttt ttttatttgt attttaatgg ttggctacgt ttttggctac
    26941 gtttggaatt attcgcccat aaattttgca aatcggtcac cagttttctt aacagattgt
    27001 ggtgtgacat gagcatagat attcatggta gttttaatat ctttatgacc taatcgttcc
    27061 tgtacttctt ttatcgacgc accactttca aacaataagc tacagtgggt atgcctaaat
    27121 ccatgtggag taatttgagg ctccatcggt gtctttttgt aaatccagtc taaccaatcg
    27181 tttactactt gggggtagta caacttgttt gtgtttgtag tgaaaagaaa ctgttttggt
    27241 gaattcgtgt tatagccata taagatcatt attttcattt gttcttgttg ccaaatacgc
    27301 agttgtttaa gtgtaaaatc atcaagctga atcgttcttg atgaattttt agttttaggt
    27361 acttgtaaaa ctacttgatt aaactcgtct tgagcgatag ttttaccaat tgttattgtt
    27421 ttgttgaaat agtctatatc ttcccattga agcgccaatg cctcgctttt acgcattcct
    27481 gtataagcta ataaacggaa aaaagtgtac attttaactg aataattttc tttagtattc
    27541 tgtaaaaaca gttgtaattg atctttcgtg taaaacttca agggttgttc ttcttcaatt
    27601 tttcttggaa gcaatgtttt agacatagga ttatccttac agacttccat tgctactcca
    27661 aatttaaaaa tttgagcagt tgtttttctc aagaaaccat attgttttaa tgttttgtgc
    27721 cattcattca cctgtttttg gcaatagcta acacttattt tatccaatcg taattcccca
    27781 aaagcaggta atatatggtt tttgcaaaaa cgttgagcaa cggcaacaga agacggtttt
    27841 actcttgttc tgtagttatc aatccataat tcatacaact cttgaaaagt tgtaacattg
    27901 attttaaact cttgtttttc tttaaagtct acaagtagtc tattaagtgc tgtttgcgcc
    27961 tcagcttttg atttgaatct tctacgcttt gtacgtatat cttttccagt cttgggatct
    28021 ttccctaaat aagtttcaaa tccccaatat ttttcgccat tttgtaattt ataactaaaa
    28081 aatgttgcca tttgattctc tccttacttc attattgcag gtagagaagt tatttgaaga
    28141 atcaattatt tttaaattta tcgtgattat cagttaaaaa ttttgcgatt atactatttt
    28201 tctcatatgg gaaattgtat tttttactaa gatctgaaat aaatttttct gtttcaaatt
    28261 ggtttttagt aattttataa aagaaatcct cgacagatga atactttgaa atatctttat
    28321 aggctaagtc catttcgtct aatagctgtt ttaaagaatc aatcattctt tctttttcac
    28381 cgtatttgaa atcaccattt cttatcttgt cttgaagttt actgttggct cttttgaaaa
    28441 atagctcttt agagatagag tgtctttggt tagcgtactc ttcaatcaga ggattgatat
    28501 tattagcaag tatctcatt
//
LOCUS       DAIGOK010000024        27825 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.024, whole genome shotgun sequence.
ACCESSION   DAIGOK010000024 DAIGOK010000000
VERSION     DAIGOK010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.024"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            14..1273
                     /gene="lysA"
                     /locus_tag="NOA01_000818"
     CDS             14..1273
                     /gene="lysA"
                     /locus_tag="NOA01_000818"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCI0632036.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            1260..2060
                     /gene="dapF"
                     /locus_tag="NOA01_000819"
     CDS             1260..2060
                     /gene="dapF"
                     /locus_tag="NOA01_000819"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCI0632037.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            2063..2944
                     /locus_tag="NOA01_000820"
     CDS             2063..2944
                     /locus_tag="NOA01_000820"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCI0632038.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            2941..3657
                     /locus_tag="NOA01_000821"
     CDS             2941..3657
                     /locus_tag="NOA01_000821"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCI0632039.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            3663..4358
                     /gene="dapD"
                     /locus_tag="NOA01_000822"
     CDS             3663..4358
                     /gene="dapD"
                     /locus_tag="NOA01_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HCI0632040.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            4374..5375
                     /locus_tag="NOA01_000823"
     CDS             4374..5375
                     /locus_tag="NOA01_000823"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCI0632041.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            5372..6733
                     /locus_tag="NOA01_000824"
     CDS             5372..6733
                     /locus_tag="NOA01_000824"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCI0632042.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYGTVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            6693..7844
                     /locus_tag="NOA01_000825"
     CDS             6693..7844
                     /locus_tag="NOA01_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCI0632043.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            7819..8988
                     /locus_tag="NOA01_000826"
     CDS             7819..8988
                     /locus_tag="NOA01_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCI0632044.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            9163..10542
                     /locus_tag="NOA01_000827"
     CDS             9163..10542
                     /locus_tag="NOA01_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCI0632045.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            10823..11620
                     /locus_tag="NOA01_000828"
                     /pseudo
     CDS             10823..11620
                     /locus_tag="NOA01_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11809..12477
                     /locus_tag="NOA01_000829"
     CDS             11809..12477
                     /locus_tag="NOA01_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HCI0632046.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(12677..12907)
                     /locus_tag="NOA01_000830"
     CDS             complement(12677..12907)
                     /locus_tag="NOA01_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632047.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(13034..14416)
                     /locus_tag="NOA01_000831"
     CDS             complement(13034..14416)
                     /locus_tag="NOA01_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCI0632048.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(14572..15759)
                     /locus_tag="NOA01_000832"
     CDS             complement(14572..15759)
                     /locus_tag="NOA01_000832"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCI0632049.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(15766..16797)
                     /locus_tag="NOA01_000833"
     CDS             complement(15766..16797)
                     /locus_tag="NOA01_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCI0632050.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(16813..18267)
                     /locus_tag="NOA01_000834"
     CDS             complement(16813..18267)
                     /locus_tag="NOA01_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCI0632051.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            18442..19347
                     /locus_tag="NOA01_000835"
     CDS             18442..19347
                     /locus_tag="NOA01_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCI0632052.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            19438..20115
                     /locus_tag="NOA01_000836"
     CDS             19438..20115
                     /locus_tag="NOA01_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCI0632053.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            20112..20282
                     /locus_tag="NOA01_000837"
     CDS             20112..20282
                     /locus_tag="NOA01_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632054.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            20396..21292
                     /locus_tag="NOA01_000838"
     CDS             20396..21292
                     /locus_tag="NOA01_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCI0632055.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(21347..22717)
                     /locus_tag="NOA01_000839"
     CDS             complement(21347..22717)
                     /locus_tag="NOA01_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCI0632056.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            22955..23701
                     /locus_tag="NOA01_000840"
     CDS             22955..23701
                     /locus_tag="NOA01_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCI0632057.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            23698..24537
                     /locus_tag="NOA01_000841"
     CDS             23698..24537
                     /locus_tag="NOA01_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCI0632058.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            24538..25488
                     /locus_tag="NOA01_000842"
     CDS             24538..25488
                     /locus_tag="NOA01_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCI0632059.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            25629..26381
                     /locus_tag="NOA01_000843"
     CDS             25629..26381
                     /locus_tag="NOA01_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632060.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            26400..27551
                     /gene="queG"
                     /locus_tag="NOA01_000844"
     CDS             26400..27551
                     /gene="queG"
                     /locus_tag="NOA01_000844"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCI0632061.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         9128 a         5049 c         5843 g         7805 t
ORIGIN      
        1 gaggaatgaa aaaatgacag aattgataat tggcggagtc acagcaagca cattggcaga
       61 aaagtataag acacctctgt atgtgtatga tgaagaaaag atgagacaaa cgatgctgtc
      121 attcaagcga ggctttgtat cgaaacgttt tgatacaaaa gtgctttacg cctctaaagc
      181 attccaaacg attgagatgc tgcatttagt tcgtgaatat ggattcgggc tagatgttgt
      241 cagtggtggg gaaatctata ccgctttgca agctgatttt cctagagata ctatctactt
      301 ccatggtaac aacaagacgc cagaggaact gatctatgca cttgaaaatg atttgttgca
      361 tattgttgcc gataatttga tggaagttga attattagca gaattaagcc aacgctattt
      421 aaaaaaaatc tgtatcatgc ttcggttgaa tgtcggagta gaagcacata ctcatgaata
      481 tatcgtcact gcgcatattg attcaaaatt cggcatgagc tatgaaagta aagagtgcca
      541 acaagccctt cagttgataa aagaaagcag ctatctagag ttagaaggat ttcatgctca
      601 tatcgggtca caaatttttg atgttactgc ttggttagct gaaatcgaca aattgacgaa
      661 ttacttaacc gattttgacc aacctctttc cttgaatctt ggcggagggt ttgggattcg
      721 ttatacacaa aatgatcagc cgatcccagt cgaagaggca ctaaaaaaac tgattgaata
      781 tacggaagaa gctttggaaa aacgaaaact gaccattcgt caattactga tcgaaccggg
      841 tagaagtatt gttggagaag ctggtacaac actatacacg attggtttca tcaaaaagac
      901 accacataaa aaatattact ttgtagatgg cgggatgaca gataatatcc gtccagctct
      961 gtatcaggca gaatacgatt gtgatttagc agcaaaactg tcggaagaaa aaacagagaa
     1021 agtgacgatt gcaggaaaaa tgtgtgaatc aggcgatgtg gtaattaaag aagcttattt
     1081 gccgaaagca gaatcgggtg atcttttagc tgtttattcc acgggagctt atggatactc
     1141 gatgagcagc aactacaatc gagcgacacg accagcagtt gtttttgtca ataatggcca
     1201 atcaagactg attgttcgca gacaagcatt ttctgatcta ctaagggggg aagtttccta
     1261 tgatccttca taaatataat ggctgcggaa atgattttat cttattagat tacgcagaag
     1321 acacagatta tagtcaatta gccgcttctt tatgcacaaa agaaaaatat gatatagacg
     1381 gattgatcgc cgtaaaaatc gatccgttgg aaatgatcta ttacaacaaa gatggcagcc
     1441 gcgcgccgat gtgcggaaat ggcattcgct gttttgctcg atacgtaaat gatcagaaca
     1501 tgatgaatga acaggaattc aacgtagaga cattagcagg cacaagaaaa attacggtgt
     1561 tggatccctc acctttttat tgcagcgtag atatgggagt gccagatgat tctccggaaa
     1621 aaatcaaagc taatcaaact tatccaataa aaaaccagga aatagagatc aatggtgaaa
     1681 aagtaacgat cactagttta tttttaggaa cgattcatac cgttgttttt gttgaaaatg
     1741 cgcgtgagga agtctggaga aaaagaggtg aactgatctg tcatcatcct ttgttcaaag
     1801 aaaaaaccaa tgtcaacttt gtagaagtag tcaatgaaca agaattgatc gtccgtacct
     1861 atgaaagagg agtaggttgg acacttgctt gcggtacagg ttgctgtgcc tcttatgtgg
     1921 ttgctaagga aactgggaaa atttcagcag atcaggtagt tgttcacttg gaacaaggag
     1981 acttgatgat ctctggtgag gatgcgatac aaatggctgg cccagcagtt catgaatgga
     2041 caagaatatt ggaggaatga agatgataaa aggttcgatc gtggcgctga tcacgccgat
     2101 gaatgaagag ggatcagtag attatgctgg tttggaaaaa ttgatccaat tccatttaga
     2161 tgaacaaaca gatggattat tagttttagg tacaacagga gaatcttcta cgctcacaca
     2221 atctgaggag gagcagatcc ttcaactgac agtaaagaaa gtggctggcc gtgtgccggt
     2281 gattgcagga gctggaacga ataatacgaa agaaacgatt gaaaaagcga agcattttgc
     2341 ttctttgggg gcagacgcac ttttggtcat caccccttac tacaacaaaa caagtgatgc
     2401 aggactagct gctcatttta cagcaattgc agaagcctca ccgattccgc ttattttgta
     2461 taatgttcct tctagaacag gcatgtcgat tccaatccat gtattagtaa atttagcaga
     2521 acatcctaat atcattggtt tgaaagaagc ctcaggagat atggcttatg tgatggatgc
     2581 cgctcggttg attggagaag aattcttcct ttacagtgga aatgatgatt tgatcctacc
     2641 agtaatgagt gtaggaggaa gcggtgtaat aagtgtatgg gcgaatatcc aacctaaaat
     2701 agtccatgaa ttagtaaaag atacgcagga cggaagatgg cagcaagcaa aagaaaagca
     2761 attaaatgca cttgaactga tccatgcctt gttcagtgag accaatccta ttcctgtcaa
     2821 agcagccatg tcattattag acttaccttc cggacctcta cgcctgcctt tagtttctct
     2881 ttcaaaagag aaaaagaaac aattagctca gcttttgtta aaagaaagga cggttgaaaa
     2941 atgaaaatcg gactgattgg cagtggacag atgggaaata gagtcgaaga agttgcacaa
     3001 gaaagaggag atacggtcct tcttttacgg acggcaccta aagaagcaac tacgcttgct
     3061 tttacggaac tgccagaact gcttattgat ttttctcatc cagataattt agagaaaatc
     3121 ctttcgtata gcttatcata tcaacttccg ttagtcattg gcacgacagg ttatacacaa
     3181 tcacagttcc aagaaataaa agaacatgcc aaacatgtac ctgtgatgta tagtgctaat
     3241 ttttcgttcg gcatcatggt gatgaatcaa ctaatcaagc aagcagctgc aatgttgcaa
     3301 ggctggcaga tcgaactgat tgaaaaacat catagtcgta aaaaagatgc accatctggt
     3361 actgcaaata tgctgcttca aacaatccaa gaagtgcaaa agctgcagcc cgtatacaac
     3421 tggcaaggga aaaaaagaga agaaaaccaa attggtgtac acagtattcg tgcaggttct
     3481 ttacctggtg aacatgatgt attgtttgct gctactgatg aaatatttac gatcaaacat
     3541 gaatcttttt ctaatcgaat ctttgctgca ggcgcagtag aagcagcgga ctggttgaaa
     3601 gatcagtcac caggttatta caaattagaa gatttattaa tggacaaagg ggtgtaaaaa
     3661 acgtggaagc tcaagaaatc atccgctaca ttcaaacggc atctaaaaag acgcctgtga
     3721 aagtctatct gaatgtaacc gagccaatcg aatttaaaga aagtaaagtt tttggtgaag
     3781 gaagcagctt cgttgttttt ggtgactatg agacgattgc tccggtttta gaagctgaat
     3841 cagaaaaaat cattgaaatc gaaatcgaaa ctgctgcaag atacagcgca gttcctttgc
     3901 tagatattaa aaaaattaat gctcgtatcg aaccaggtgc tattatcaga gatcaagtgt
     3961 caatcggcaa caatgccgtg atcatgatgg gagcgattat caatattggt gcagtaatcg
     4021 gtgaaaacac gatgattgat atgggtgctg tcttaggtgg acgagcgaca gtgggcaaaa
     4081 actgccatat tggtgcagga gctgtcttag caggtgtgat cgaaccagca tcggccaaac
     4141 cggttatcgt agaagacggc gtactggtag gtgcgaatgc agtgattgtt gaaggtgtcc
     4201 atatcggaaa agatgcagtc gtggcagcag gagcagtggt tttagaagat gtggcagcag
     4261 aaactgttgt cggtggaata cctgcacgtg tattaaaaat aagtgatgat cagacaaaag
     4321 aaaatacagc attgattgct gcgttacgcg aattatagag gagatggaga aaaatgcgcg
     4381 aaatagatac ctgtattgtg ggggcaaccg gattagtcgg ccaaacgatg ctcaaagtgt
     4441 tagaagaata tgattttcca gtaggacgat tgaaattatt agcttcaaaa cgttcagccg
     4501 gtaaagtttg tgtattcaag aaaaagaaac acactgtaca ggaattgaca gaaacttcat
     4561 ttgaaggata tgatttagcg ctattttctg caggtgcttc tgtatccaaa gagtttgcgc
     4621 caatcgcacg aagtcaggga ctatttgtca tagataattc ctcagcatgg cgggaagatt
     4681 cggatattcc attaatcgtt ccagaggtca atctggaaga ctatacattg aatcggctga
     4741 ttgcaaatcc aaattgttca accattcaag cggtacttcc gcttaaagct ttacaagaag
     4801 cctttggtat ccagcgagtc aattattcga cctatcaagc agtttctggt tccgggcaaa
     4861 aaggcattga agatcttcat cgtacgcaaa atgacgagga accagttttg tatccttata
     4921 atattagcca aacggtgatt ccggaaatcg atcagccttt ggaaaatggg tatacaaaag
     4981 aagaacagaa aatgataaat gagacccgta agattctgca tcagcctgct ttgccagtat
     5041 cagcaacctg cgtacgtgtg ccaatcgaaa atggacatgg agtctctgtg gcagttcaac
     5101 tgcaaaaaga gtttacgacg gaagaaatta gagagtgtct cagccagttt ccagggattc
     5161 aattagaaga tgatctagca gcacataaat atccgacgag cattcttgct agaggaacag
     5221 atatggttta tgttgggagg atcagaaaag acacttcttt agagaacgga ttgttgttct
     5281 atacgactgc cgataacata cgcaaaggag ccgctgcgaa tgccgtacag attgctgcag
     5341 ctttaatgca gaaaatagcg gaggtggcaa gatgagtaat acgctgaaag tagcgaaatt
     5401 cggcggaaca tcgatgtctc atgaagagca attcaaaaaa gtccgtacaa ttgttctttc
     5461 agatcccaat cgccaagtga tcgttgtttc tgctttaggc aaacgtacgt cagatgatga
     5521 caaagtaaca gacttacttt atttgattta tgcttacctt cagcatcatg ttgattggac
     5581 acccctttgg aaaaggattt gtgatcgttt cattcaagta agagatgttc ttgccctgca
     5641 gttacctatt gaagatgaac tagcacgcat acagaaacaa ctggaagacg aaaagataac
     5701 tgaagattat ttagtaagta gaggtgaata tctgactgct caactgatgg ctgagtatct
     5761 tggttaccag tttattgacg caaaagacct gattttcttt gactatgatg ggaaaatcga
     5821 tcaggaaaaa acagcacagg aaatcacaaa agcatttaaa agatatccga aaatggttgt
     5881 tccagggttt tacggggcga accctgcagg aaaagaaaaa ttattgggcc gtgggggaag
     5941 cgacattacc ggcgcgatac tggcgaagat gttgcaagcg gaaagttatg agaattggac
     6001 ggatgtttca ggaatcatga tggctgatcc gcgaatcatc gatcatccta aaatcatcga
     6061 ggaaatctct tttagagaac tgagagagat ggcttatatg ggggctaacg ttcttcatga
     6121 agcagctgtt ttcccagttc aagaggctga tatccccatc caaataaaaa ataccaatga
     6181 tcctttcgct caagggacaa aaatatcgaa tcacgaaata gaaaaggaaa atggactgac
     6241 aggaatagct ggacgcaagg attttctttc catcacttta ttcaaacgtc atatgtccga
     6301 tgaaatcggg tttatctgga aagcaatgag catttttgct aaacatgaga tatcaatcga
     6361 acatattcct tcagggatcg acaatatcgg agtgatcgtc tcagcagaag cgattgccga
     6421 taagttattt ctcatcacga aagaattaaa agaaacactt ggcgtggaag aaatcgtcgt
     6481 gatcgaagac ttagctttga tctctgtcgt tgggggtccg cataaagaat tgattggatt
     6541 atctgggaaa gtgttatcga tattgaacaa attagaaatt cgcacatcga tcttatcaca
     6601 aggagcacag gagttgaatc tgatcatcgg tgtaccgaat aatcaatatg gaacagtagt
     6661 gaagggtatc tatgaaggga tggtgaatac ggatgctgaa agaatatcga atggcactcc
     6721 atcaaattcc tgaaatcggg tttcatgaat tcaagacaaa aaaatattta tatgaacaag
     6781 taaaaaacag cgaggccgtt atccatgaaa tcggcgaaac aggactactc ctttattttg
     6841 ataatcagca agaagaaacg attgcttttc gaacagatat cgatgcactg ccagtcaaag
     6901 aagaaacgaa tctctctttt gcttctaagc atgaaggtta tatgcatgca tgtggtcatg
     6961 acggacatat ggcgatgctt ttagggttga ctgactttat aactaaacac attgaggatc
     7021 ttatttataa tgtagtactg atctatcagc catcagaaga aatttccggt ggcgcacaaa
     7081 gcgttattca gtcaggattg ttggataaat atgaagtaca agcaatattt ggctttcatc
     7141 tatggcctgg actagctaag ggagaagttt tctctcgacc aggacctttg atggcacaaa
     7201 gcagtgaaac agacattatc attaaaggcc agtctgcaca tattgcttct agtgaaaaag
     7261 gaatcgatag tctggaggca gctgtacgat ttttgaagaa tgtttatgat tttgaccggc
     7321 aattaccggc agagtggaat catctaatga aatttggcca aatccaaggt ggaacgatcc
     7381 gaaatgtttt agctggagaa gttattgtat ccggaagtat ccgttcttac agttcagata
     7441 tgcaaaatta tttgaagagg ggattagacc atttagcaga acagtttcac tctacagcta
     7501 ctatttcttt tcgctataat gacggctatc cagctgtaat caaccataga ggcttatatg
     7561 cagcactgtt agattcaaac aaactacatg aattagatgc tccagttctt caagccgaag
     7621 attttggcat atatacgcaa caatatccat gtgtattttt ctttttaggt gtcggtgata
     7681 cccctgcttt acatgatccg acgttcgatt ttgatatgga tgttttagaa aatggtgtag
     7741 aatggtacaa aacgattctt tttacagaaa atcttcttgt aaaaatcgat agcagttcaa
     7801 aagaggagcg agtaaataat gatgaattta tccaatcgat ttagtcctag gctttctaga
     7861 atagaagtat caaaaatacg acaattcgat cagcaaatat cgtcgatacc agatgtgatc
     7921 aaactcaccc tgggagaacc ggattttcca actcctgaac atgtcaagca agcaggaata
     7981 gcagcgattg aagaagactt ttctcactat accggaatga gaggattaga ggaactgcgg
     8041 gaagcggctt gtatatttca gcaacagcgt tatggattaa cttatgatcc gcaaacggaa
     8101 gttttgacga cagttggtgc aacggaagcg attgcctctg cgttgttatc tgttttggaa
     8161 gaaggagaca aggttcttat tccagcgcct gcttattcag gatatcagcc attagtggaa
     8221 ctagctggtg ctgaattgat cccgatagat acttctgaca cagggtttgt ctgtcagccg
     8281 gaacaatttg aacgagcatt tgaacaatat ggatcagctg tcaaagcagt cattttaaac
     8341 tatcctaaca atcctactgg gacgacattg tctgcaaaac aattgaaagc aatcgctgag
     8401 gttttaaaaa aatatccggt atttgttata agtgatgaag tatatgctga attgacttat
     8461 agtggaacgc atatgtccat agctacttat cttccagaac aaacaatcgt gataagcggg
     8521 ttatccaagt cacatgcgat gactggttgg cgagtgggct ttatttttgc tcaaaaagcc
     8581 cttattgacg aactaattaa agtccatcaa tatctggtga ctgccgctac aacgatgtcg
     8641 caaaaagctg cggctgaagc gctaataaat ggggtgaaag atagcgagaa tatgaaagag
     8701 caatatcaaa cacgaagaga ctatctggtc cagcaattgg cgcctttagg atttgaagtg
     8761 acccagccaa acggtgcatt ttacttgttt tgcaaactcc cagctgccat tcaaactgac
     8821 tcttggcagt tttgtgtaga attagcggaa caggcaaaag ttgcgtgtat cccaggaatt
     8881 gcatttggcc cagaaggaga aggttatatc cggatttctt atgctagcag catggaaaaa
     8941 ttacatgagg cctgcaaacg gatcaaagac tttttaacag aacagtgatt catagatttt
     9001 atcaaaaaaa caatcgttaa ttttgcggta tattctgcaa ggagacgatt gttttttaaa
     9061 tttagttcaa atgaacgcgt ttcttttgat tcataactaa tgtaatgtta agtttaaatt
     9121 gtttagaatg attttaaaag aatattcaag gaggtttcat caatggaaaa aatcaaagtt
     9181 gtcattgtag gtgcatctca tggagggcat caatcgatct tggaactatt gacacgatac
     9241 ggtgaaaatg tagatatcac gctatttgaa gcaggagact atatttcctt catgtcatgt
     9301 ggtatggagc tgtatttaga agacaaggta acagatgtaa atgatgtacg taatttcaga
     9361 ccagaaaact ttccgcaatc caatgttcat attttaaata atcacgaagt aaaaacgatc
     9421 gatgccgata aaaaaacagt aacggttact agcgcatcag ataacgaaac aaaagaatat
     9481 ccttatgaca aattgatttt gagttctggt gtcaaaccaa acaatctgcc agtaccagga
     9541 gcagatctag aaaatgtcta cttgatgcgt ggatacaatt gggcaaccaa aatcaaagaa
     9601 aaattaaatg atccggctat taaaaaaatc gctgtcgtag gtgcgggtta cattgggatc
     9661 gaagcagcag aagcaagccg aaaagctggg aaagaagtcg tattgcttga tgtgatcgat
     9721 agacctttag gcacttattt agatgctgag atgacagata ttttagaaca acacctaaat
     9781 gaaaacggta ttgaagtttt gacaaatgca aaaatcgaag cttttactgg taacggaaaa
     9841 gttgaggcaa tcaaaacaag tgaaaaagaa atcccagcag atctagtcat tcaagctgcc
     9901 ggtgtgaagg caaatacaga atggttaaaa gggatcgtcg acttagatga acgtggctgg
     9961 atccaaacaa atgaatactt gcaaacgaat cttccagatg tgtatgcagt aggagatgct
    10021 acacttgctt attctatccc agcacgcaaa aaaatgccga tcgctttagc gactgttgca
    10081 cgtagagaag cacgttatgt agtaaaacat ctttttgaag aagtaccaaa caaaccgttc
    10141 ggcggagtcg taggttcctc tgcattaagt gtctttgact atcattttgc agcaagcgga
    10201 ttgaatagct ttacagcagc aaaagcagga gtagatgtac gagtgtctta ttatgaagat
    10261 acgttacgac caaaatatgt accagaagaa caaggaaatc caaaagtgtc catccaatta
    10321 ttctcggatc caatgaccca tcaattgcta ggaggagcag tattatctac ctatgatgtt
    10381 acaggtcaag gaaatgtatt ggcactagcg atccaaaaca aactgacttt agaagaacta
    10441 gcagaagctg acttcttctt ccagccaggc tttgaccgac aatggagcat gttgaatcta
    10501 gccgctcaac aagcgttagg agaagaacca tttgtagaat aagaaaacga aatatttgaa
    10561 aagaggttgt gaaaaagctg ccttgcatca agtaataaga acagccaagt aaaaacagct
    10621 tcttatgttt ttaacttggc tggacttatt accgcagatg caagcttttg aacaccgttt
    10681 attttcaaaa aagggactgt gacacgattt gtgtaccgac ccccaaaagt tagaccaaaa
    10741 aatctaactt ttggaggtcg gtatttttta tggttaaata tacttttgaa cttaaattaa
    10801 agatcgttca tgattatttg gatggcaaag gcgattggac cgtaaaaatc cagatcaaga
    10861 gattgaagcg gaaatgctta aaattcggga gaagcacaag gattatggct gtttacggat
    10921 gaccaatgaa ctaagaaacc gcggtttctc tatcaataaa aagaaagttc agcgcttgat
    10981 caagaagcta ggaataaaag taacggcata cacgcgtaaa tcacgtcgct acaattctta
    11041 tcgaggaaaa gttggagcag tagccaaaaa tcggatccat cgtcgtttct acaccagtat
    11101 ctgccatcag aaaatcacga cggatacaac tgaatttaaa taccatgaag tggatgccaa
    11161 aggcattatc cgtcaaaaga aattgtatct agatcctttt atggatatgt tcaatagtga
    11221 gattttatcc tatcgtatct ctgaaaagcc caatgcatta gcggtaatgg aaggtttgga
    11281 ggaagctatc cagaccacta acgattgtcc ctatcgtcgt acgtttcact cagatcaagg
    11341 ttgggcgtat caaatgaaag cttatagaaa caaattgaag gagtacaaaa tctttcaaag
    11401 catgtctcgg aaagggaact gcttagataa ttcacttatg gagaattttt ttggcttatt
    11461 aaaacaagag atttttcacg ggaaagttta taactgtttc gaggaattaa aatcagccat
    11521 cgatagctat atctattact acaataatga acggataaaa cagaaattaa actggcaaag
    11581 tctcgtccaa tttcgtaaaa caaccgtaac ggttgtgtaa aaatatcatt ttagagcata
    11641 aaaaaaagga gtggatttct ccactccaaa aaagtctaac ttttgggggt cactacagtg
    11701 tcacagtcct ttttttagta aaaggataca tttcttcctt gtatattcca tgcgtgttcg
    11761 tgagaaagca tatataattt aattaacaga tagttagggg gagaaaaaat gattttttca
    11821 attacaatga aactggtgtt cgggttgatc ggtttattat tagtggtacg tttgcttggg
    11881 aagaaatcca tgtcagaaat cacaccgttt gatttagtct atacgcttgt tttaggtggg
    11941 atcttagaag aatcaaccta tgatgacaat gtccatgtag ggcatgtcct atttgccatt
    12001 gcgctttggg cagtaatgat ttatggtatc gaacgtatcg tccaaaaaaa tgaaaaggtc
    12061 aatcgttggg tcaaaggcga gccatctgtt ctaatcaaag acggcgtcat caacatgaca
    12121 gagttgacaa ataaccatat cgagatggag cagttgcgag caatcttaag acagcaagaa
    12181 tgcttttttc ttgaaaatgc caaacatgtt atcctagaga atgccggtca aatgagtgta
    12241 ttgaaaaaat cagatgaaga caaagcttta tcgatcctgc ttgtagatga agggcaaatt
    12301 cagcataaag tgcttcagtc caatcaattg actgaagcat ggcttatgga gaacttgaag
    12361 aaagaaggct atgcagatgt aaaacagctg atttatgttg aatggtcaga agaaaaaggg
    12421 ctctacatac taaataaaga agacgtcatg catgaatctt accgaataga tggatagata
    12481 gacagaggtt gtgaaatcag cgccttggct tgttactcaa aactaagcga ctttttcaca
    12541 acctctgata tctaatatac gttgttccag aagttgcttc ttcgaaaaat aagccaaaat
    12601 tccacaaaaa tttaagaagc aattttcgaa aaatcctttc ttattttttg aagttgatca
    12661 cttctgtcac aacctctcat gtcacaaacc atttttttgt ttcctttaac tcaatatacc
    12721 gtacgtcttc ccacaatatc ggctgatggt caatcataaa gtaatcttca taggcttcac
    12781 caatgaagtg cccttcgacc tcatccaaga gtcttccaaa ttcatctttt aaattgagct
    12841 gaatgattac tgctttacga gataaaaagg cttctgtcag tatactatcg atttcttgca
    12901 atgtcatttg ttgtttagga agaggctgtt tgcgtgcttc ttcttcattt ttttgatacc
    12961 gttgactagt tcttccatcg caaaagcagt cccccatttt aatccaaatg gccgatctcg
    13021 atactcgtta tagtcaatga atttttttgc tgtccgtctc atcttttcca ccttctaatc
    13081 cactcatacc acctgcatgc ccgccaacga gagaactacg tgcgatggcg cgtcctcctt
    13141 gcaagagact atgcgcatgg atgattgatt gaaagccgta tttttttcta atcgtatcga
    13201 caacaaaatc aactttcgct tcatttatct gcttttcagg atcttcaaaa aggttaagct
    13261 gtaaacctgt cgcgtatacg aggtcggaac agttcacgcc tacacgccga atatcctggt
    13321 agtggtaatg ctggtggaaa atggtcaata aagcatctgc tagatctctg ctagcatttg
    13381 tcgcttcgat tttcatttgc tggtgaaacc cggtcttgcc atttggatca agatagccta
    13441 aagagtatcc tacccataag ctgactactt gtgtttttgc tgcagatcgt ctcaagcgag
    13501 tgccgacttg gtcggccatt tctttgataa cgatctcgat ttcttctcgt cttgtataat
    13561 ctctatctag gatttgacta tttccgatac tttttgattt tggtgtatat tcttgtccca
    13621 aaaaacttcg atcgatgccc caagcatggg cataaagctg agtaccgatg acccctagct
    13681 tgcttttcaa gtgataataa tccgcgtgag ctaaatcata aacagaatgt atacccattt
    13741 gtttcagacg ttttgctgtt cgtttgccga ttccccaaaa gtcagtgata tctgagattt
    13801 tccataactt ttctggtaca tcttcataac gccattcagc aaacatttgc tttccgtctt
    13861 ttgagccatt gtctaaggct agtttagcaa gtagcgggtt atcgccaatt cctatagtcg
    13921 tgtagattcc ggtctgctca aacacatctt tatggatcag tttagccatg tcgtacgcat
    13981 ttgttttacc aaagagtgcc atgctatttg ttacatcaag gaagctttca tcgacggaat
    14041 agacatgatg gttcgcttcg tctgcatatt ttttatagat agaattgatt tctctgttct
    14101 tttccatata ataggccatc cttggggcaa caatatataa atcacttgga taggggaagg
    14161 gaagatctct ggcacggctg atattggtga tgccgtaagc tttttttgct gcaggactgg
    14221 aagccaagat taatccgcta ccccgtgttt ctgtcgaatc ggacggatag gacatgacta
    14281 ctaatttagt tgtaagggga tcaagatcct tttcaacgca ctctacggag gcgtaaaaag
    14341 atttgcagtc aatacataaa atatctcttg aaggttcttt ttcataaaca aataccggat
    14401 tttgattcgc ccacataaca atcacctctt caacacaatt agaacacacg ttctctttcc
    14461 gtgtcaataa aaaacagcaa ttttcaaata cgagcaaaag gctcacattt ggaaattgct
    14521 gagaggttat ttttatctat ttgtttttag ctgagatcgt tgattttttt atcaaacatg
    14581 aacagcttct ggatcgatct ttgttgtccc atttccaatg tgtgcaacat aaaattccgg
    14641 agcatagccg acaactttca tgtattcatc atagacattg tttttgaagg caggtatatt
    14701 ttcttctttg acaagagcaa tcgcacagcc accaaatcca gctcctgtca ttcgtgcacc
    14761 taagacacct tcttgttttt gagcagcatc tactagtgta tctaattcga tcccagttac
    14821 ttcataatca tagcgaagag agtggtgaga atcatttaac agttgaccaa atgcttctaa
    14881 gttcccagct tctaattcag cttttgcctt caaagtccgc tgattttcag aaacggcatg
    14941 gcgagctctg cgtatcaatg tatcatcgcc gatttggtca atagttgatt caaattcttc
    15001 ttcatctaag tctcctaaag aagagatagg caaagttttt tgcaaacgtg ccaaggcttc
    15061 ttcgcattcg ctacggcgtt cattgtattt tgaatctgcc aattcccgcc gcttattcgt
    15121 attcatgatg acgattgcat aaccatctag tttcactgga accatttcgt atttcagcgt
    15181 atttgtatct aataagattg catgatctag ctcaccgaag ccgatggcaa actgatccat
    15241 gatacctgaa ttgacaccga tgaattcatt ttctactttt ttacctgttt ggactagttc
    15301 caagcgatct gtttttaaat caaacagatc ttctaataca acacctacta ataattctaa
    15361 tgaagcgcta ctggaaagtc ctgcaccatt agggattgtt ccttccacta ataactcaaa
    15421 cccatgatcg attgagtgtc cagcttcttt taatttaagg accattcctt ttacgtaatt
    15481 tgcccagttt gcttttttat catattctag ctcatctaaa gtaaaactaa tgacaccgat
    15541 atcgtcaaag tttgtggaat aaactaatat tttattatcg tctcgaggtg ctgctacacc
    15601 ttgcgttcca taagtgatgg aagcggggaa gacatggcct ccattgtaat ctgtgtgttc
    15661 accaatcaaa ttgatgcgtc ctggtgagaa gtattgtgta gtttttttag gtccgaatgt
    15721 ttcggcaaat tttgtgttta agcttctttt gatttccatg atgagttatt cctttcttgt
    15781 tttgattttg aattctgtta ctgtttcaaa tactgatccg gcttttaaat gaatcgaacc
    15841 aaatgctgag aagcgctctg cacctggagc tgtttgagtc tcaaatgtga tgccaccgtg
    15901 atgtgccagt tttcggttgc gcatttctgg tgtttctgtg ccgaaatttg cagtgaaaat
    15961 aaccacacta gaagcatctg tagcaatatc tacttggatt cttttatcgg ggctagtcaa
    16021 gcgagcggct tctttaccca agccggtttc ttttaagaaa aatggatgat cgattccgtc
    16081 aacaagattt ttttgatcca aatcactagc aaaaacggac gctaattttt taggtatttg
    16141 aaaatcgaaa gcacttcctg cagcattttc ttttacccct ataggaatac tatctgttcg
    16201 caaaggagca taagcttcgc tattgagcca aagctcatgt tgatcgattg actgagaagc
    16261 atctcccgtc aagttgaagt aaacatgatt tgtcggattg aataaagtat cttggtcact
    16321 ggtgccgcga gaggtcactc gccagatatt atcttttgtc aaagtatagc ggatttccac
    16381 atccattgta cctggaaaac catgttctcc gtcgggactt gtagtagtaa aaatgacaga
    16441 agcttcattt tcgccattca aaatgacata agaccatttt tttaattcaa atccaggagc
    16501 accaccatgc agattatgtc cagtttttgg atctgtggct aattggtaag tttttccatt
    16561 gagagaaaag cggccatttt cgattctccc tgcggtgcga ccgatagaag caccgatata
    16621 cggatctttt tcgatatatt cttcagcggt gtcaaatccc aaaactaatt ctcgatcatt
    16681 tgattttaaa ctgacgattc tagcacctaa atcagagata gaaagtgcta gcttgttttt
    16741 gttctccaag gttatcaagt gataaccttg gccaaaatct ttttctgtaa tcttcatttt
    16801 caagatcctt tcttaatggg aaggaactac tgcatcgtga agttcatctt ccattgtatc
    16861 agctggagcc aatttgtttt ccaatttctc aacgatttgt gcatgttttt tctctgtcaa
    16921 tgtaatcttc caagcaaaaa taatagcgga gaaaatcatc aaagcagcag gagcatagaa
    16981 tgcataagct ttgaaagtcg ttacgccatg agcagtgata tcagcagcag tagcattacc
    17041 agtcatccca gaagcgatag cgacaaaccc tacgatcccg ttagacaaag caccagcgat
    17101 tttatctagt aaagggcgaa ttgatagagt aacagcttcg ttacggatac catttttcca
    17161 ttgaccatat tcaacagagt ctgtgatcgt catcaaagcg gataagaaaa tcaattgttg
    17221 agggaagtag aacaatacta gcccgatgat aacgattggc aaacttgaac ctgcgatcgt
    17281 aaagaaaaag taagaagaaa tcatcattgc gatccctcca aggtaaacgt agcgacgaga
    17341 gatgacgcgt gtaagcattg ggaatagtgg aacagctact aaaccaagta aggcagcaac
    17401 tagaccaacg atccaaaatt cagttggttg gccaagtaca tatttgaaga agtagaataa
    17461 tacggcagtt gttgctacat tggcaatcgc ataagcgata taagcaaggg caagccacat
    17521 caactgatca ttttgagcaa ctgctttgaa cacatctttg atcccagctt tttcattttg
    17581 ctgacggatc gcagtatcga cttcttttgt gcccaaggca gtgataatcg cagtaccgcc
    17641 tgaaataaca gcagcaatca caccgaacca gaaccagcct gaagcgcctt caatatgttt
    17701 atgagtaaca agataggtaa agaaagtaac gatagggaca acgactagag tagtaccatt
    17761 tgcacctaga gaagagccga aacgagcaaa cgtagcaaat ttttcacgtt cctttgtttc
    17821 cgtactcaaa gctggaatca ttgaccaaaa agcgatatcc ttaaatgaat aaaaactatc
    17881 caataagatg aaaacgattg caaataatat gataaataaa gtcgtattat gagtagctaa
    17941 gccaaagaaa ttggtaaaga ttgctaccaa gagtacggaa ctaacaacgc cgccgataac
    18001 taaccatggt ttgaatttac cgtattttgt acgagtatta tcgatgatgc caccaatcaa
    18061 aggatcaaaa ataatttcaa ctaagcggat acctacaact aagcttgtaa ctgtagcaat
    18121 catttttgca ttcgttgcct tatctgttcc tgcgaacatc tggcttgtca cgaataccat
    18181 aaaataagtg cttaatgtaa catagaatgt gtcgtgtcca aaagcgccta aagcgtagga
    18241 cacacgttgg atcattttgc ttttcataat ctgtattcct ctcctgttca atatataata
    18301 ctaagtaaac cggttacaac gaataacaaa gatcttttaa tgacttaaac ttaacaagaa
    18361 atcgcttcct ttttaatata tttctgtgac ttttatttaa ctttttgttg cattatacta
    18421 aaggagtaag gagggataga cgtggagcaa tcgatttttt taaaaaagga acatcaacaa
    18481 gtagaaaaag aattgtattt tgatttttgt ggtttctcta aaacgatgcc gtttcattct
    18541 tttggtcctg ctattcgcaa tgattacatc ctgcatgtcg tgatggaagg caaaggatct
    18601 tatcatgtga aaaatcaaca attccaattg aaaaaaggtg atctgttctt gattcgtcca
    18661 ggagactcga cgttttactt atcagatgga gaagatccgt ggatgtattg ctggctttca
    18721 tttggtggga gtgctgcaga agaaatcatt cggcagtcgc ttttcagaga agaccaatac
    18781 acgatggtct cttctgacat acaaaaatat atcgacatta ttttagaatg catgaaagat
    18841 acggaagaaa ccttggaaaa tgaattacag ttaacagcat taacctaccg ttttttatca
    18901 gtccttttaa aagatggcgg aaaggtcagc ggtggtgtac aaaaggttca ttcctcttta
    18961 gctgttgaaa cagtcaaata catcgaggca cattatgctg aaaaattgtc tgttgaggaa
    19021 atcgcccagc atttagcggt caatcggagc catttgtcgc gagtattcaa aaatcatatg
    19081 ggtaccagta tcaaggaata tttgatcggg gtacggatca atcgaggggc gtttcttctt
    19141 tctctgacgg atgaatccgt ggaaagtatt gcctatcaag tagggttcaa tagtctagtc
    19201 gtatttagcc gaatgttcaa aaagagtacc ggagaaacgg ctacgaacta tcgtaaacgt
    19261 atgaaaaacg aaaattttaa gaatattgct ttggatacct tgaaaaaaca gttggaaaaa
    19321 caaaccgcag tttcttggac aacctgatta gtctggttaa aatgacaagc atcggcaaaa
    19381 gaaacttgtg gcggttatgc tacactgtaa agcgaagact aaaaaggagg aaaagggatg
    19441 cgtacactta tcatcattag ccatccaacg atcgaagaat ctttaaatca gcaatttttc
    19501 aaagaagctt ctgctggttt accagctact tggcatcatt tggaatcaag ttatccagac
    19561 ggaaaaatag atatagagac tgaactacag ctcttggccc agcatgatcg gatcattttc
    19621 cagtttccgt tttactggta tagttctcct gctcatttaa aaatttggca agataatgtc
    19681 ttggaacgag cagcacatgt attaaaaggg aaagaactag gaatcgtatt gacaacaggt
    19741 gtggcggaaa aagaatacca agctggagga aaagaagaat atacgatttc cgaatttttg
    19801 aggccgtatc agcgaatcgc aaataaattc catatgacct atcttccgcc attcgtttta
    19861 gcacaattta tgtatttatc ccaggaaaaa cgttgggaga agctaattgc ttatcagcag
    19921 tatctctcat tggaaggcaa accatcgctg actcagcgga ttgactggtt tattcaaaga
    19981 gtacaggaaa atcagaaaat gcaggaagaa gaatctgaaa aacaagcata tatcgtagaa
    20041 gcactgactg aggcaaaaga gcagatcgaa gacttgacct ttacactgca agaaatgaaa
    20101 ggaacttcat tatgacaaat gattggctga tccaacaatt agatgaatta agcgaaaaat
    20161 cagaatatca agaccgtgcg ctcttatatg aaataaagaa actgatggct gagatagacg
    20221 aacgaacaat gcagctgcaa ggagaaatcg acggacaatc ttggaatcat ctcaactggt
    20281 gaaattttct tataaataac aaagataaaa atgtgcacag cgacaattat ttgctacaat
    20341 aagaatatga agcgtttgat aacgctatta tacataaaaa ttggtggtga aaaaaatgta
    20401 tgatgaaaat gagtggggag aaaatgttcg gatgacccga agaagacagc aaaaacaggc
    20461 agctcgtaag cggatgatca tgattggggc agcagttgcc gtgtttttga tcatagcagg
    20521 aggagttgcg ggtctatatt ttcatggaaa aaattcgtca gaagcgaaaa gtacgaccat
    20581 aacggaaaca acgacaaaga aaacagagac gaaacaaaca agtgagtcat caactaaaac
    20641 aaaagagaca caaacttctg aagaaaaaac agcaaatggt ttgccagagc aacttcaagc
    20701 aatcgtaaat gattattcga aaaagatcga agaaaagacg cctgcattga ttgaagaata
    20761 ccagacagaa attcagggaa atcaagaggg aatagctggt ttatcagcag ttgctaacca
    20821 aaaagcacgt gagcttcaag caatctccga cgaagggatc agaaaattac gtgcagcgta
    20881 tcaggcagca gaaaataaag acggtgtaga cttagatact ttgatcaatc aattatcggc
    20941 taattatacg aatcatgtcg ctaagatttc cgatatttat ttgcagacta gcgcctctct
    21001 tcaagcagag tcaacaagca cacaagacac aacatcttct gattctgaga cagcagagtc
    21061 ggtagaagct tctgaaatgc aagctcgaac aacacaagac acaacagatt cctctgaaac
    21121 taataccaac agcgcgactt cacaggctgc gacaaccgta gttttggaag gagaaggtcc
    21181 aaatcaaata gccgaacgaa ctggcgtacc agtagaaacg atcttgtcgc tgaatgggat
    21241 gagcatggag aattatttcc tgactcccgg tgagacgcta aaattgaatt gataaaagca
    21301 ataaaaaaga acgaagcttc cactaaggtg aagcttcgtt ctttttttat tttccggttc
    21361 ttttttcgcg tacaaagtaa tggatcgtca tgtaaagcaa gaaaacgagt ccaataatca
    21421 atgagattct cgtttcagga ttgataaaca tgaaaatcaa tacgatagct aaaaaagcaa
    21481 gtgtcacata attcgtataa ggagatagtg gcattttaaa aggatgatca tgcattttat
    21541 ctgcgtgctt ctttttgaag cgaatctcgc tgatcaaaat cactacccaa ggaaccatac
    21601 caggcaaaat acttgagctg tatacgaaaa cgaaaatatt ttctgactgg ttgatgaaca
    21661 ttggtagaag ataatttaag atcagaccaa ttaagattcc acctgagatt gctacgacag
    21721 gataaaaagg cgcaccgtga cgagatagtt tcagaaattt tttagacatc tttccattta
    21781 tggcaagtgt atagaccata cgacttgcgc tgaaaatccc tgaattgcaa cctgataatg
    21841 ccgctgtaat cacgacaaaa ttaatcagtc ctgcagcaaa tgtaatgccg actttagaaa
    21901 aagtttgtac aaaaggtgaa ccaatctcac tcaattgatt ccacggatag atactcacaa
    21961 tgataaaaat agcaccaata taaaaaatca agattcgacc gatagtggac tggacagctt
    22021 caatgatcgt ctttttagga ttctctgctt caccagcagt cattccgatc agttcgattc
    22081 cttgatagga agcaacaacg atcgataatg caaagaaaaa tcctttgacg cctccagtga
    22141 agaatccacc atttttccaa agatttgaaa tccctactgc ctgaccatga ttaccaaatc
    22201 caaataagat taccccaaga cctgcaatga tcatcaaaat gatcgttatg actttgatga
    22261 tcgaaaacca aaattctaat tcgccaaacg ccttgacaga aataagattt gccagcataa
    22321 gaaacaagac gacaatgatt ccgggaataa ttgccggcaa atcaggccac caataattca
    22381 tataagatcc tacagcaatc acttcactta ttcctacgac aatatattga aaaatattac
    22441 tccaagcggt caagtagcca gccacaggat ggatgtactc gctcgcgtaa tttgcaaaag
    22501 aaccagtcga aggatcaacg taaagcattt cgcctaaagc acgcataatc aaatacaaaa
    22561 agaatcctgc aatcgcatag gctcccatca ctgagggccc tgtccaacgt atggttgagg
    22621 aggcacccat aaacagaccg acaccgatcg ttccacctag tgcgatcatt tggatatgac
    22681 gggcactaag acttcgagtt aacttttctt cttccataca aaaactcctt tcgttgttga
    22741 gtcaacaaga aaaaataatc aaacgtttca acacatgttt ttcattatta ggttagcctt
    22801 attgaactct tgatgtgatt ctacagaaaa ttttcataaa atgcgaggaa aattcgaaaa
    22861 aaactttttc atcattctac ttgaaaatgc tttccatttt agttaaacta tatttaaata
    22921 aaaagttagg taaacctaaa aaggagtgta agatttggaa cgtacggcca ttgtaattca
    22981 agacctttca gccgcttatc aagggaaaac tgttttgaaa actatcaact tgacgataaa
    23041 tccacaaaaa attaccggta ttatcggacc aaacggtgca ggaaaatcaa catttatgaa
    23101 atctttgcta gagttgatac cagctactac tggtaaagtc accttttctg gacagtcggt
    23161 aaactcagtc cgtaaaaaaa tagcctacgt ggaacagcga agtgaattgg atctttcctt
    23221 tccaattgat gtattaggcg ttgtactttt aggaacatat ccatctttac gaattggaca
    23281 aagacctggg aaaaaagaaa aagaacgtgc aagacaagct ttgaaaaaag tagggatgga
    23341 agaatatgcc aaaagacaga tcagcgaact atcgggtgga cagcttcaga gagtttttat
    23401 tgcaagagct ctagcccaag gagcagaatg gatcttttta gatgaaccat tcgtagggat
    23461 tgatgcgtta agtgaacgaa agatctttga catcttgcag gaattgaaga attcaggaaa
    23521 aacgattttg atcgtccatc atgatcttca taaagtagaa gaatatttcg atgaggttat
    23581 tcttttaaat aaacagctga tcgcttccgg tccagtacaa gaagtcttta catcgaagaa
    23641 ccttcaattg gcctatggtg agatcatcag acatttagta aaaggaggag agaaaaaatg
    23701 atccaatcct ttattgatgg attaatgaat tatcaattcc tgcaatacgc tttggtcact
    23761 tccatgcttg taggacttgc ttctggagtc atcggttctt ttattatttt acgagggatg
    23821 tctttgatgg gagacgctat ctctcacgca gttcttccag gtgtagccat ttcttatatg
    23881 tttggtttta gttacatctt tggagccact gcttttggga tgctaacagc agcagtgatt
    23941 ggtttcgtga ctcagcggag ccgattgaaa aatgatacag cgattggaat tgtctttagc
    24001 tccttttttg cattagggat tatcttgatt tcatatgccc agagtgcgac agatttgtat
    24061 catattttat ttgggaatgt cttagctgtt agggaaagcg atttgttgct gacagcactc
    24121 gttagtggaa tcgtcttgat atttgtcttt ttcttttata aagaattgaa gatcacatca
    24181 ttcgatccga caatggcaaa agcttatggg ctgaacacat cgctgatcca ttatcttttg
    24241 atgttctttt tgacattagt ggctgtagtc agtttacaga cagtaggaac aatcttggtg
    24301 attgccatgt tgatcacacc agccgccacg gcttacttgc taacgaacca tttactgaaa
    24361 atgatcatta cagctgcagg aatcggtatg ctaagtgcag ttgtcggtgt gtttttcagt
    24421 tatagttaca attggccatc aggagctacg atcgtgttag catgtaccgc attttttatc
    24481 cttgcttttt tattttctcc aacaaaagga attttatttc agaaaggaag cagatagatg
    24541 aaaacaaaaa aatcactctt tttaatcttg gcagttagtt tcttggtatt agccggttgc
    24601 ggaaagcagg cttctgacca agcggatgaa ggaagtaaag aaaagttgtc agtcgtggct
    24661 accaattcga tcttggcgga catggcaaaa gaggtcggaa cagatcaaat agatatccac
    24721 agtatcgtac cagtcggaac agatccgcat gaatatgaag tactaccaga agacatcaaa
    24781 aaggcaagcg atgcagatgt tattttatac aacggtttga atcttgaaac aggtaacagc
    24841 tggttcgata acttgatgga aacggctaaa aaagaagaag gaaaagatta ttttgcagtt
    24901 agcaaaaatg tagaacctct ttatttaact agcggtgaag aacatacaaa agcagatccc
    24961 catgcttggc tagacctatc taacggaata aaatatgtgg aggaaatcgc acgtatattc
    25021 tctgaaaaag atgcagaaaa tgcgacactc tataaaaaaa atgcagaagc atatgtggaa
    25081 aaactaaaag aattagatac acaagcgaag gaaagttttg cttctatcga agagaacaaa
    25141 aaattattag taacaagtga aggggcattc aagtattttt cacgagcata tgatttgcca
    25201 gcagcttata tatgggagat caatacagaa agtcaaggta cgcctgatca aatgaaagcg
    25261 atcattgatc agataagagc aaaagaagta ccggttttat tcgtggaaac cagtgtagac
    25321 tcaagaagca tggaacgggt agcaaaagaa acgggtttga aaatctatga taaactgttc
    25381 actgattcca tagcaaaaga aggagaacaa ggagattctt actatcagat gatgaaatgg
    25441 aatattgaaa cgatccatga aggtttgtca caaacaaaag aaagctgaaa agtgcaaggt
    25501 ctagcttgag gagcagacct tgcatttttt ataagagata tgaaaaaaaa ttatgctgtt
    25561 tttcgtcgta cagatacctt ttttgaagaa attccgttat agttagagta aggaaggaag
    25621 gtgtggctat gaattttaca cgtaaagata aatatacgat taaagaagaa gtctttcgat
    25681 tgatcagcca gcaggaaaat accgacaagg atcaagacct tcaatttctt gaagcagaat
    25741 tagctaaatt attcatagca aatcaaaatg agacaacaga accagatgaa gagctgtaca
    25801 taggcaaatg gttgtttgat gaatttcagc ctttagatga aaataccggt atcggttatt
    25861 ttgtgttcct ttctccagtc ggcttcacac ctggcgtatt agttaaacaa aacgacgaaa
    25921 taaccgtcaa agcatggcct gcattatttt cagtcatcaa agcagaagga gtaatcaaaa
    25981 agatccagtc ggcctattat aaaagtcaat ttattgccaa cagcaaatta atgaatatgt
    26041 tttataatcg tctagtagaa attgctaggc ctgggaaaat cccgcaaaca ttcacgtaca
    26101 cacagcaaaa aaatctctat gaagaagtat tgtattcccg cgatctgtct gagttttcgc
    26161 catgggaaga ggtaaggctc catgaactgt taagtacaaa accagtttca ttgaattttt
    26221 ttaatgcatt agataacaaa agaaaattaa tttcaacaaa ttattgggaa gtatttgctg
    26281 caagaaaaaa atggatggaa gaaaagaaga ttgccgatca gcagatccag tttgtccagc
    26341 tgaaaaaaga aaaactatat tatgggttat acagccgtta acagcaagcg aggaagacca
    26401 tgacattaaa agaagaaatc atacaagaaa gcaaacgatt aggaattgac aaaatcgggt
    26461 tcaccacggc ggaacctttt gattcattaa aagaatcgtt ggaagaacaa aaagcagctg
    26521 gccatacttc tggctttgaa catccaaata tcgatgagcg tctttatcca gaaatgacat
    26581 ttgatcagcc gaaatcgatc atttccattg cacttgcgta tcctactagg atccatgaag
    26641 aagtgcctag ggatgaaaaa cgcgggcagt ttgctcgtgc ttcttggggg atcgattacc
    26701 atgatatact cagagaccgt ttgaaccgat tgatcgaatt catccaaaca agagcagaaa
    26761 aatggcaaaa agaagaggaa tggcgtctag ctccgcaagt agatactgga gaactgatcg
    26821 atgtcgctgt agcacagcga gctggtttag gatttattgg acgaaacgga ttgttgatca
    26881 ctgaagagtt cggttcattt gtctatcttg gtgagatcgt tactaatatt gtatttgagc
    26941 ctgataaacc aggagaattt ggttgcggtg actgtatgcg ctgcgtgact gcttgtccga
    27001 caaaagcatt gcttggtgac ggaagaatga atgcccagcg atgtttgtct tatcaaactc
    27061 agacaaaagg gatgatgccg gaagaatatc gtaaaaaaat gcgaaatgtc atttatggat
    27121 gtgatatctg ccagttagtc tgcccttaca atcaaggaaa agattttcat tttcatgaag
    27181 agatggaacc aaaagtagac gaaatatatc caaaattaaa gccaatgctt tcgatgtcta
    27241 ataaagactt caaacagcaa tttgggcatt tagctggttc atggcgtggg aagaaacctt
    27301 tgcagagaaa cgcattgatt gctttagcta atctaggtga tcgaacggca ttgcctgaga
    27361 tttcagaatg tttaacagat gtccgccctg tcatcagagg aacagcagct tgggctattg
    27421 gacgtctagg aacaaaagaa ccggaaaaat ggcttgatgt attagatgaa atattagcta
    27481 aagaaacaga agaagtcgtt ataaacgaat tggaacatgc ccagaaatta ttgcggagaa
    27541 agttaaaata aaaaaatagt taacattatt caaagttttt gtccagtaat tgttgtaaca
    27601 attactggtt tttttgtatt ttttacattt ttttaagaga atttgataaa aatcaaaaat
    27661 ttttttgctt ttttttatgg attattgcag tgaatgttac agaatctgcg tttttcattc
    27721 aaaaataaaa aaacatagaa aaacgattac tttgcttata caacaaggct tttatggttt
    27781 tgtatcaaaa aaatatttgt ttaaatgtta caaatattaa ataat
//
LOCUS       DAIGOK010000025        27381 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.025, whole genome shotgun sequence.
ACCESSION   DAIGOK010000025 DAIGOK010000000
VERSION     DAIGOK010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.025"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            154..384
                     /locus_tag="NOA01_000845"
     CDS             154..384
                     /locus_tag="NOA01_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HCI0632062.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            481..1782
                     /gene="murA"
                     /locus_tag="NOA01_000846"
     CDS             481..1782
                     /gene="murA"
                     /locus_tag="NOA01_000846"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCI0632063.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1795..1971
                     /locus_tag="NOA01_000847"
     CDS             1795..1971
                     /locus_tag="NOA01_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCI0632064.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2106..2402
                     /gene="yidD"
                     /locus_tag="NOA01_000848"
     CDS             2106..2402
                     /gene="yidD"
                     /locus_tag="NOA01_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCI0632065.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2586..3053
                     /locus_tag="NOA01_000849"
     CDS             2586..3053
                     /locus_tag="NOA01_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCI0632066.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3138..4022
                     /locus_tag="NOA01_000850"
     CDS             3138..4022
                     /locus_tag="NOA01_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCI0632067.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4136..4372
                     /locus_tag="NOA01_000851"
     CDS             4136..4372
                     /locus_tag="NOA01_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HCI0632068.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4460..4540
                     /locus_tag="NOA01_000852"
     tRNA            4460..4540
                     /locus_tag="NOA01_000852"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4494..4496,aa:Tyr,seq:gta)
     gene            4547..4618
                     /locus_tag="NOA01_000853"
     tRNA            4547..4618
                     /locus_tag="NOA01_000853"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4579..4581,aa:Gln,seq:ttg)
     gene            4743..4913
                     /locus_tag="NOA01_000854"
     CDS             4743..4913
                     /locus_tag="NOA01_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632069.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            5133..5840
                     /locus_tag="NOA01_000855"
     CDS             5133..5840
                     /locus_tag="NOA01_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCI0632070.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5900..6544)
                     /locus_tag="NOA01_000856"
     CDS             complement(5900..6544)
                     /locus_tag="NOA01_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HCI0632071.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6671..6958
                     /locus_tag="NOA01_000857"
     CDS             6671..6958
                     /locus_tag="NOA01_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632072.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7066..7386
                     /locus_tag="NOA01_000858"
     CDS             7066..7386
                     /locus_tag="NOA01_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCI0632073.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7467..8780
                     /locus_tag="NOA01_000859"
     CDS             7467..8780
                     /locus_tag="NOA01_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCI0632074.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8777..9496
                     /locus_tag="NOA01_000860"
     CDS             8777..9496
                     /locus_tag="NOA01_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HCI0632075.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9624..10175
                     /gene="raiA"
                     /locus_tag="NOA01_000861"
     CDS             9624..10175
                     /gene="raiA"
                     /locus_tag="NOA01_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCI0632076.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10397..12931
                     /gene="secA"
                     /locus_tag="NOA01_000862"
     CDS             10397..12931
                     /gene="secA"
                     /locus_tag="NOA01_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCI0632077.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDIQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGDE
                     DAVIQSKMLTKQVEAAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIVDFAGSTLVHEDSISLTDIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKDAN"
     gene            13058..14159
                     /gene="prfB"
                     /locus_tag="NOA01_000863"
     CDS             join(13058..13129,13131..14159)
                     /gene="prfB"
                     /locus_tag="NOA01_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCI0632078.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAVMQEEAEARI
                     NALDEKMNTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14264..14950
                     /gene="ftsE"
                     /locus_tag="NOA01_000864"
     CDS             14264..14950
                     /gene="ftsE"
                     /locus_tag="NOA01_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCI0632079.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14934..15827
                     /locus_tag="NOA01_000865"
     CDS             14934..15827
                     /locus_tag="NOA01_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI0632080.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEKAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFYQLINPSLLRSHYSLIHPENIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15979..17427
                     /gene="cls"
                     /locus_tag="NOA01_000866"
     CDS             15979..17427
                     /gene="cls"
                     /locus_tag="NOA01_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCI0632081.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDTLGE
                     EVREALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPEFFPTIHSTGDMAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEVYENRGMMIKMKEGLARLISPLL"
     gene            17764..18621
                     /locus_tag="NOA01_000867"
     CDS             17764..18621
                     /locus_tag="NOA01_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCI0632082.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQAENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18736..19656
                     /gene="pstC"
                     /locus_tag="NOA01_000868"
     CDS             18736..19656
                     /gene="pstC"
                     /locus_tag="NOA01_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HCI0632083.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19656..20540
                     /gene="pstA"
                     /locus_tag="NOA01_000869"
     CDS             19656..20540
                     /gene="pstA"
                     /locus_tag="NOA01_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCI0632084.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20555..21361
                     /gene="pstB"
                     /locus_tag="NOA01_000870"
     CDS             20555..21361
                     /gene="pstB"
                     /locus_tag="NOA01_000870"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCI0632085.1"
                     /translation="MKKYNWNETNVITMPKDKEVALYTNDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYKDVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21377..22135
                     /locus_tag="NOA01_000871"
     CDS             21377..22135
                     /locus_tag="NOA01_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCI0632086.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWEDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22148..22825
                     /gene="phoU"
                     /locus_tag="NOA01_000872"
     CDS             22148..22825
                     /gene="phoU"
                     /locus_tag="NOA01_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCI0632087.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            23047..24081
                     /locus_tag="NOA01_000873"
     CDS             23047..24081
                     /locus_tag="NOA01_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCI0632088.1"
                     /translation="MQKKISIIITLFFICILASWFIQRQAALYDVPVIEIERVRIEED
                     QQLITGRLMNTDQKGEVISLTAPYQKNQIETANITTRQIYLVQMHGDEWQLVAKKNDG
                     WLFFFTSVFIGTMLLIGGKQGVFALGSLIINIVLLLLLLLLFTKTEGISLLNIAILFV
                     VIGIIVSILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTR
                     PYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKQQPKISAKQLWLSGRQVGRDVIGS
                     MANILLFVYISGAIPSLLLYLGNQWSFKETVERHLSLELLRVIAGSLGIVLSIPIAVL
                     FFLAVRRLKK"
     gene            24078..24845
                     /locus_tag="NOA01_000874"
     CDS             24078..24845
                     /locus_tag="NOA01_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCI0632089.1"
                     /translation="MNVLIILVGIFAISVLFVGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILVLSIIGFLLIAVVILFFINGYNLKMRAAFASILIFLFCFLLLIPITDFLAI
                     QGFTSIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     MSFNQLRHASFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDISFQQIINEKSFVL
                     EYTMAILTTLAALLVLPLTCVTTAYLFTKKKEHLKME"
     gene            24945..26513
                     /gene="liaX"
                     /locus_tag="NOA01_000875"
     CDS             24945..26513
                     /gene="liaX"
                     /locus_tag="NOA01_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HCI0632090.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDELENGEEKISEPVDPKEKERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEATGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSDDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVNQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATPENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDSIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            26689..26997
                     /locus_tag="NOA01_000876"
     CDS             26689..26997
                     /locus_tag="NOA01_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HCI0632091.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTKKRKEAEKIDDDEWSDF
                     "
     gene            26997..27347
                     /locus_tag="NOA01_000877"
     CDS             26997..27347
                     /locus_tag="NOA01_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCI0632092.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALFVAILM
                     SLVNVIVSEHNSRK"
BASE COUNT         9041 a         4669 c         5937 g         7734 t
ORIGIN      
        1 cagtcccttt ttttgcatat ttttagcatt tatttcctaa aaaatgtaag gggattccta
       61 tatttttaag tatacttgtt tttcctttta tacattttat ggtattatca ttttattagt
      121 tattataaaa caatcgttag aaaggacgtc attatgcaag tatacggaat tgatgcggtg
      181 cttcgaatcg tgagtcattt ggcgtttatc tacttagcct tctggtcttt acgttcgtta
      241 cgcattgaaa cttttttcag accaatgcat aatacgcaaa ttagaatggc aatcgtactt
      301 ttttcaattt ttttagggta cactgcaagt aattttttct tagaagttat cgctttatgc
      361 cgaaatcttt tcgttagcct gtaaatttta aaaaaaggta ctattttaaa agcaaggatg
      421 ttataataga aaagattttg agtaagtgta tgctcattgg aattttttgg agggaacaac
      481 atggaagaga tcatcgtaag aggtggcaat caattaaatg gaacagtaag aattgaagga
      541 gctaaaaatg ctgttttgcc tattttagct gctagtttgt tagcagaaga aggtattacg
      601 acattagaca acgttccgat tctttctgat gtcttcacta tgaatcaagt gattcgtcac
      661 ttaaacgtag atgtagattt tgatgaacaa aagaatcaag taacaatcga tgcttcaaga
      721 caattagaaa tcgaagcgcc ttatgagtat gttagtcaaa tgcgtgcttc aatcgttgtg
      781 atgggtccgc tacttgccag aaatggacat gcaaaagtag ctatgcctgg tggatgtgcg
      841 attggtaaac gaccaatcga tttgcattta aaaggatttc aagctttagg agcaaaaatt
      901 attcaaaaaa acggctatat cgaagcaatt gcggatgaat tgatcggtaa cacgatttat
      961 ttagatttcc ctagtgtagg tgctactcaa aatatcatga tggctgccgt aaaagcaaaa
     1021 ggaacaacca tcattgaaaa cgtagctcgc gaaccagaaa tcgttgattt agcaaacatt
     1081 ttgaataaaa tgggcgctca agtctatgga gctggtactg aaacaatgcg tattgaaggc
     1141 gtggatcatt tacatgcagt caaccactca atcgtacaag atcgtatcga agcaggtacc
     1201 tttatggttg ctgctgcaat gacacaaggc aatgtgctga ttgcagatgc tatctctgaa
     1261 cataaccgcc cattgatttc taaattgatc gaaatgggtg cagaaatcat tgaagaagaa
     1321 ggcggagtcc gtgtcatcgg accaaaacat atcttaccta cagatgtaaa aacgatgcct
     1381 catcctggtt tccctacaga tatgcaagct caaatgacag ctatccaatt agttgcagaa
     1441 ggtacaagtg tagtgactga aacagtgttt gaaaatcgtt tccaacattt agaagagatg
     1501 cgccgtatga acgctcatgt caaaatcgac ggaaatgtag caatcatgga tggaaaccat
     1561 gaattgcaag gtgcagaagt atatgcaaca gacttgcggg cagcagcagc attagtttta
     1621 gctggcttga aagcaaatgg aatcacacgt gtgcgtaact tgaattatct agaccgtgga
     1681 tattataatt tccatatcaa actacagcaa ttaggtgcag acgttgaacg tgtagacatg
     1741 gatcaaactt ctgctgaaaa aacagcacaa acgattgctt aaggaagtgg ctgaatgagt
     1801 aaccaacgat atatcttatt aactttgata aagattttag tggtgatctt attgcttatc
     1861 cttctttttg tggccggaac gatgatcgga tatggtgtca tcggtggcgg caatccattt
     1921 aaagtattcc agccaagttt gtggattcat attcgagact tttttcattg ataaataaga
     1981 atcacagtgg ttctagtcga tatgaagtga gccatgaagc agaacatgac aaaagaaaac
     2041 agaagggaac gttgacgtta gtcgcgatcc ctttttttag taaaagaaaa gaaggaatct
     2101 aattgatgaa gaagatattg atttatttag ttagaggata tcagcgcttt atttcgcctg
     2161 catttccacc tagctgccgt tattacccaa cctgttccaa ttatatgata aaagcaattc
     2221 aaacccatgg tgcactaaaa gggacggtaa tggggactgc acggatctta cgctgtcatc
     2281 cttttgtcaa aggcggtatc gattatgtac cattgacatt ccaattaacg cgaaataaga
     2341 aagataaaaa agaaccagat tataaaaagg ttgtgaaaaa tacgagtagc actgagcgat
     2401 aaagaaacaa tttggaaaat agcttcttat attttgcaaa attgtgagtt attgccgaag
     2461 tgttggattt tgaacaccgt caaaaaaagg ttgtgaaaaa tacgggtagc actgagcgat
     2521 aaagaaaaag aggaaaatta ttgtcaaagt aacaactccg caactactta tatgggggac
     2581 aaaaaatgat cgaagaacta aacaagaaag atttaccatg gtccttgtta ttagatgcag
     2641 atcctgataa agaaaaagtt caagtatatg tttccagagg gagtggctgg atatggaaag
     2701 aaaaagaaaa aacaataggt gtactgatct atgtggcaag ggaaaaagaa tacgaaatcg
     2761 tcaatgtagc agtagcccca acgtatcaag gaaaggggat tggcggaaaa ttacttgaga
     2821 cagcttttca gaagctttca caactcgctt ccagtcaaac tcagatcatc atacgaaccg
     2881 gcagtactag cagtgcggcg ttacatctct atcaaaaaat gggctatgtg gagattggtc
     2941 gagtcaaaga ctattttatc catcattact ctgagcccat ttttgaaaat ggagatcaat
     3001 taagggatca agtaatttta gcaattcact tacctaaaaa tagctttaac taataaactt
     3061 tcaattcgag aaaatgcttt caaatgctaa atagatacat tacaatagct ttagcaaaca
     3121 aaaaggagaa tatttttatg ggaatattag tttttgattt tggtggatcg gctgtcaaat
     3181 atggttgttg ggatggcaaa gagattaaag gtaagggaca atttgcaacg cctgaatctt
     3241 gggaagaaat gaaagcgcaa ttatttcaag tttataaaaa aatactcgtt tcttttgaag
     3301 tcgaaggaat tggaatcagc tctcctggag tagtgaataa tgagaaacag gtgattgagg
     3361 gaatcagtgc gattccttat attcatggat ttaatatttt ccgagatttg gaaactctgt
     3421 ttcaactccc agtaacgatt gaaaatgacg ccaactgtgc cggaatggct gaattttatg
     3481 aaggtgctgc ccgtgattat caagatgttg cttttgttgt ggcaggaact gggattggcg
     3541 gggcgttgtt cacgaaagga caaatcaata aaggggcaca tctttatggt ggagaatttg
     3601 ggttgatgtt cttaaatggc gataagacat tcagtaaact tggtacagcg gtacagatgg
     3661 cttggcgtta ttgtgaacgc aaaggtctcg ataaaaattc ttataccgga aaagatgtct
     3721 ttgaattagc agaaactgga gatgcaatag caaaagaaga agttgagagt ttttatacgt
     3781 atcttacaaa gggattattc agtattcaat tttcgtttga tccagaagtg atcgtattag
     3841 gcggcggtgt ttctgcaaaa gaagggctca ttgatgagat caaaagccga atgaagaagc
     3901 tgacagaaca attcgatctg catgatttcg aaccgcagat tttattatgt gaatacagaa
     3961 acgatgctaa tcttgtaggt gcagcagcaa actatctcgc tgtacgtcaa ggggagaatt
     4021 aagagcttta gagaaggtgc agcttgcata aaagaagtga atgaaatgaa taggagattt
     4081 ttacctaagg aatgttttat ttgctgcagt atgataaaat atctgagagg tgaaaatgat
     4141 gtcaaaaaaa aataaagatg ttgaagtgcg tatagaagaa gtgaaaaaag atatcaaagg
     4201 taagaattat gaagtcactc agttatttat agggaaaaaa atgattggtg agatcttagc
     4261 ctatgggcca aaagaatatg aaattttctt tggtgaagaa gattttggaa aagaaaaaag
     4321 tcttgaaaat gcgatagaaa cagtgattcg acactggaat ctgcacgaat aaaaaaatat
     4381 caaaaaaact ttcaataaag ggttgcaaaa atctggaaaa ttaggtatta taactaagtc
     4441 aggttgatac aaacctttcg gaggagtagc gaagtggcta aacgcgacgg actgtaaatc
     4501 cgttccttag ggttcagtgg ttcgaatcca ctctcctcca tttcattggg gtatagccaa
     4561 gcggtaaggc aagggacttt gactccctca tgcgttggtt cgaatccagc taccccagta
     4621 taccacaatc gtaagctctc gttagttcga gagctctttt ttttgctttg ctaccttgtt
     4681 ccttgaagat tctgctgatt taagatagag ttaagttaac tacaagaatt ggagggatcc
     4741 acatgaaatg gaaagaaaaa gtagacgaag cagctgaaaa gttatatgat ttggtcaaat
     4801 cagaaaaata caatattgaa gtgaatattc cgaaaaaagg cggaaaagcg gtccaggtaa
     4861 aatcaaaacg cccaacgaat catacaaaaa aatggatggc taaaaaccgc taataataac
     4921 aaaagatcca taagtcggca ttttcatcga ttttagggtc ttttgttatt ttctaggttg
     4981 aacatatcta aaaaattgtt tctgacaata ggagtaggaa gaacgatgtg tgtctaaatc
     5041 ctctgttgat ggatagaaac ctttatcttt ttcagttgtg aatcaggtat ataccagtta
     5101 taatagaaga aacgaaagga cgggtgtacg ttatgtcgaa tcaattacct gtatatatac
     5161 agattcatga ccaattaaaa aatgaaatcg aacaaggtat atggaaagtc ggagaccgtc
     5221 ttccttcaga aagagaatta gcagtgaaat tcaatgtcag ccgtatgacg ttgagacaag
     5281 caattcaaaa cttggcagat gaaggaatct tagaaagaaa gatcggttcg ggtacatatg
     5341 ttgcgcgaga aaaagtacaa gaaaaaatgt ctggtgcaac aagttttaca gaaattatgg
     5401 aatcgcaaaa tcgagtaccg tctagtcgga caatctctta tttcttgact tcgccaagct
     5461 caagtgaaat ggaaaaacta aacttgagta aagatgacac gatcttgcga atggaacgta
     5521 ttcgtttcgc agatgatgtc cctatttgtt ttgaagttgc cagtgtgcca caaaagctga
     5581 tcgatggcta cagcaaagca gagattactg aatcgttgta tcgcacgcta gaagaaaaag
     5641 gcgggaaaaa gatcggtcca gcgaatcaaa cgattactgc tgtgttggct tctgagaaaa
     5701 ttgctgacta tttagaggta aaacgaggag atgcagttct tcgtttgcgc cagatttctt
     5761 attttgaaga cggtacgccg tttgagtatg tgcgtacgca atacgtagga aatcgttttg
     5821 agttttattt ggaaaaataa aaaacaggtg atccctgatt ccttacttag aaataaaaga
     5881 gaatcagaat cacctgtttt tatatatgat cagttaccag ttgctcgtga taagaaacag
     5941 gaccctcttc aaattggact tgtccgttca gcagctcaat gatggattct ttaaagtggt
     6001 cagcactttt ttcatctaca gcacagatga cgctaacttg atcagtatat tggatttctt
     6061 ttataaaaaa atgttggagt tcaagataat tctgtaattt tcctagttgc ggatacgtga
     6121 tcatcacaga gatctcctgt tgcaaggtac cttctacgat tccaatgtca gacaatgctt
     6181 gagagacagc atgagtgtat gctcgtatta atccgcctgt gccgagtttc gttcctccaa
     6241 aatatcgagt gactactgca gctacattga tcagttgatt ttttttgagg acttccaaca
     6301 tcggtacgcc agccgttcca cttggttctc catcatcgct gcttcgctgg atttcatttt
     6361 tttctccaat gatgaaggcg ctgcagttat ggttcgcttt ccaatgttct ttttttattt
     6421 cttgaatgaa actttttgct tggtcctctg attctattcg tctaagagaa cagataaagc
     6481 gtgatttttt gatctcaatc tcactttgtc cattttcttt tattgtacga tagcgctcga
     6541 tcatcttgtc acctcttttt ttcattttct ttattcagta taaataaagt tctaggttgt
     6601 ttcaacactg taacggttgc gcaatcgctg tagtatgcga taataaagac aaaggtaggt
     6661 ggatctgggg atgcaggaac gaatgaaaaa gtacgatgca attacgcatt atttgaagaa
     6721 caatggcggc tctcaagtaa cgttgacctt tacccaattt gacgaactgc tgtttcccag
     6781 taacggactt cccaaaacag caagggaaag tacagattgg tgggcaaatg attataaaca
     6841 ccctgaaaaa ggtgcctatg gctggataaa tgcaggctat gaagtagttg tcatcaattt
     6901 ggacaaagaa tatgtcgtgt tcaataaatt agtgaaatct agttggctat ttgattaaat
     6961 aaaccgcgtt tagaaaacgt tgacaaattt ctttcttttt atataataga gtaacagacg
     7021 cgaagaaaac gtttgcgtag tttgttttaa aaaggagaga aaagtatggc taaaaaaaca
     7081 atcatgcttg tatgttcggc tggtatgagc acaagtttac tagtaacaaa aatgcaaaaa
     7141 gctgctgaag caaaaggaat cgactcggat attttcgctg tgtccgcttc tgacgcagat
     7201 aacaatcttg caaataaaga tgtagatgta ctgttattag gacctcaagt tcgctttatg
     7261 aaagctgatt ttgaaaaacg tctggaacca aaaggcattc ctttagacgt catcaatatg
     7321 gctgattatg gtatgatgaa tggcgaaaaa gtgttagatc aagcaatcac gctcataggt
     7381 gaataaatag aacagtataa ctatcgaaca agcgatccaa aaaatagatc gcttgttttt
     7441 tcgtatattt ttttagggcg aataatatga atcatttttg gggtagaagg cttctgacga
     7501 gagatattaa agcaatagac tgtgaggagg taaatttacc aaattatgag aagatcgcag
     7561 cagtggaacg tttgggcaac tggattcttt gtcatcgctg tggaatgagg acgtccgtca
     7621 ttgatggaca gcttgcagat tacggttatt tttgtacaca ttgtctatat ttagggcgat
     7681 gcgacactca acaagattta tatttgtttg atcagcctga agcaaaacct cgagaagtcg
     7741 tctttttttg gacaggacag cttactgaaa aacaaacgga aatcgcaaag agaatacttt
     7801 gccattcaga aaaaagacat catcttattt gggctgtaac aggtgcaggg aaaacagaga
     7861 tgctttatcc tattttagtg gaaactttaa aagctggagg aagagtagcg atttgtacac
     7921 cgcgagtcga tgtttgtaat gaattgtttt tacgctatcg ttatgttttt ccagaagaaa
     7981 aaatcacgtt gctccatggg aatacagcaa cggcttatac ctttacttct tttgttatct
     8041 gtacgattca tcagctatac cgtttttatc ggtcctttga tttaatagtg atcgatgaga
     8101 tcgatgcgtt tccttataac ggggatgcag gattagctca tgctgtgcaa acagcgatta
     8161 aaccagacgg acgattcatc tacttaagtg ccacacctga taaaaagtta ctaaaggaaa
     8221 tcaagcagac gtttgacatc cacaaacttg ctttgcgctt tcatcggcgt ttacttcctg
     8281 aacccgtctt ggttttcatg aataattggc gacaaaaatg caagaacaag aagaagataa
     8341 aatctttggt gcggatcatt catcaattaa ttaaagaaaa ccatatcctt ttattttgtc
     8401 caagtatcac gatgatgcat cgtttgaaag ccacacttca agaggctctt ccgagatatg
     8461 agatcacaga tgtttcttca agagacaatc agcgggcgga aaaagtccag aaaatgcgag
     8521 aagcaaaata tgacatactg ttaaccacga tgatccttga acgtggagtc acatttgaaa
     8581 gaatatccgt tatcgtttta ggagcggatc atccggtttt tactaaatct tctttggtac
     8641 aaatcgcagg aagagccgat cgtaaagggc cttatacgaa cagtcaagta tactttttct
     8701 atgaagaaaa aactgcagct attcgaaaag cttgtcagga aatcaaagaa atgaatgaag
     8761 aagggaagaa gatgctgtga ggtgcagttg ctgcagtaaa gatatcgtcc aaaatttaac
     8821 attcagcgag attattggtg tttcgaaata cagattacct cagacttgtt cagtttgtcg
     8881 cgcacaattt catttattgg aaacaagagg ctgttctggc tgtcaaaaac caatccattt
     8941 caatctagag agtacgaaaa agaaagtaaa gtctattccc gtttactgca gtgactgtca
     9001 agaatggaag aagaaatatc cgacctatga ttttcagcac cgtgcctttt ttgtgtacga
     9061 tgaaaatatg aaggcctggc tcaatcaata caagtttatg ggagatatcc gactagcagg
     9121 cacctttgca tataaatgga cgcagataaa aaaagaatac ccgacataca tctattgtcc
     9181 aataccttta tctgaaaaaa ggcgaacaga aagaggattc aatcaagtaa gcgagatgtt
     9241 gaaagcagca agaattcctt ttcagatgct actcaaaaga gagaagcatc tgctggctca
     9301 agcaaaaaac acgcgaaagg aaagattgga gatgcctcaa ccttttgaac ttgcagtcac
     9361 cttttctaag atacaaaata aaaaaatatt gattattgat gatgtctata caacaggaca
     9421 gactcttttc cacgcagcag attgtttgca ggtcgcacag ccgaaaatca tccgaaccat
     9481 ttctttagca agatgaatta gatgtggaaa tttgatagcg atttaatttg caaataacct
     9541 agagttcgat ataatagaat taagaagagg gaaagagtgg tccttctcct tcttgattgt
     9601 ggtagacgaa aggggtaatt tttatgttta gatataatgt acgcggcgaa aatatcgaag
     9661 taactgaagc gattcgcgac tacgttgaga aaaaagtagg caaactagaa cgctatttca
     9721 gtgacgcacc cgatgcaacg gcgcatgtga acttaaaagt ttatacagaa aaaacagcaa
     9781 aagtggaagt aacgatccca ttaccttatc ttgtattacg tgccgaagaa acttctccag
     9841 atttatacgc aagtatcgat ttggttgttg acaaattgga acgccaaatc cgcaaattca
     9901 aaacaaaaat taatcgtaaa tcccgtgaaa cgggcatgaa tactgcagat gctactgctt
     9961 tattcaatga agaggacgaa aacgaaaatg attctgaatt agatatcgta cgtacgaaac
    10021 gcctttcatt gaaaccaatg gatagtgaag aagcagtctt acaaatgaac atgctgggac
    10081 ataacttctt tatttttgaa gatgcagaaa caaatggtac aagtatcgtt taccgccgta
    10141 aagatggaaa atacggactg attgaaacag actgatcagc cttacttaaa taacaacgaa
    10201 aacttgcagc agctactgac agctgccgca agtttttttg agtttcctcc tcttttagat
    10261 gaaatatgaa taaattatga agttcttact ttaatacgct taagatattt caattatcta
    10321 taactaatga taaaatgaaa agtaaagtct acatagtctg gataatacac agaaggaaaa
    10381 ggagattaca caacgcatgg ccaatttgat tcgttcactt atcgaaaacg ataagaaaga
    10441 actaaaacgt ttagatagca ttgcaaaaaa agttgaagca tacgctgacc aaatggcagc
    10501 attaactgat gaacaattac aggcaaaaac tgctgaattc aagggcagat accaaaaagg
    10561 agaaacatta gatcaattac ttccggaagc ttttgcagtc gtccgagaag cagcaaaacg
    10621 tgtattgggg ttatatccat accacgtaca attaatgggt gggatcgtgc ttcacgatgg
    10681 gaatatccct gaaatgcgta ctggtgaagg taaaaccttg actgcaacga tgccggtgta
    10741 tctgaatgcg ttgactggcg aaggggtcca cgtagttacc gtcaatgaat atttagcaac
    10801 ccgtgactcg acggaaatgg gcgagcttta taatttctta ggattgacag ttgggttgaa
    10861 tatcaactca aaatcttcgg atgaaaaacg cgaggcttat aattgcgata tcacttatag
    10921 cacgaataat gaattaggat ttgactattt gagagacaac atggtcgttt accgtcatca
    10981 aatggtacaa cgtccgctga attatgccat tgtcgatgaa gttgactcga tcttgattga
    11041 tgaagcgaga acgccgctga tcatttcagg gcaagcagaa aaatcaacag ctttatatac
    11101 aagagcagac aacttcgtga aacgtttgaa agaagaagaa gattataaaa tcgatataca
    11161 atcaaaaaca atcagtttaa ctgaagcagg gatcgaaaaa gctgaagaaa cattcggttt
    11221 ggacaactta tatgacattg aaaatactgc attgacccat cacttggatc aagcattgag
    11281 agccaactac atcatgcttt tagacatcga ttatgttgtt caagatggaa aagtattgat
    11341 tgttgaccaa tttacgggtc ggatcatgga tggacgccgt tattcagatg gtcttcatca
    11401 agcgatcgaa gctaaagaag gcgtagagat cgaagacgaa acgaaaacga tggcgacgat
    11461 cactttccag aactatttcc gtatgtacaa gaaacttgcg gggatgactg gtacagctaa
    11521 gacagaagaa gaagagttca gagagattta taatattcaa gtataccaga ttccgacaaa
    11581 ccgtccggtc atccgtgacg atcgaccaga tttgctttac ccaacattac aaagcaagtt
    11641 ccatgctgtc gtacaagata tcaaagatcg ctaccgcaaa ggacagcctg tcttagtcgg
    11701 aactgttgca gttgaaacat ctgagttatt atcagatatg ctgaatcaag aacgtattcc
    11761 tcatgaagta ctgaatgcga aaaatcactt taaagaagca gaaatcatca tgaatgcggg
    11821 tcaaaaaggt gcagtgacga ttgctaccaa catggctggt cggggaacag atatcaagct
    11881 agggcttggc gtaagagaac ttggtggcct agcagttatc gggacagaac gacatgaatc
    11941 tcgtcgtatc gataaccagc ttcgaggacg tgcaggacgt caaggagacc ctggtatgtc
    12001 acaattctac ttgtctttag aagatgattt gatgaaacga ttcggttcag aacgaatcaa
    12061 agctttctta gatcgaatga agatcggtga cgaagatgct gtgatccaaa gcaagatgct
    12121 gacgaaacaa gtagaagctg cacaaaaacg cgtagaagga aacaactacg acacacgtaa
    12181 gaacgtcttg caatacgatg acgtgatgcg tgagcaacgt gaagtaatct ataaacagcg
    12241 tcaagaagtg atcatggaag aaaaaagctt atctaaacca ctgatgaata tggtcaaacg
    12301 gacaatcagt cgtgtggtag atgcgcatac gcaattagaa aaagaaaatt ggaatttgga
    12361 aagtatcgtc gattttgctg gcagcacgct tgtccatgaa gacagtattt cccttacaga
    12421 tatcgaagga aaaacctctc aagaaatcaa agaatatcta aacgaacgag cacaagaagt
    12481 attcaatacc aaagcagctc aattgaacgg accagagcaa ctgcttgaat ttgaaaaagt
    12541 cgtgatctta cgtgtcgtgg actctaaatg gacagatcat attgatgcaa tggaccaatt
    12601 acgtcagtca atcggcttgc gtgcctatgg tcaaaacaat ccattagtcg aataccaaac
    12661 agaaggttat aagatgttcg aagatatggt aggtgccatt gaatatgaag tcactcgtat
    12721 cttcatgaaa gctgagatcc gtcaaaacgt acaacgtgag caagtttctc aaggaacagc
    12781 ttctcactca gaagatggag atgccaaaga aaacagtaat actagcgcga aaaaacaacc
    12841 aatccatgtg aacaaggttg gtagaaatga tccttgtcct tgtggaagcg ggaaaaaata
    12901 taagaactgt cacggaaaag atgcaaacta atttctgaca gcaggattgg tgagacaaat
    12961 tcttttttaa agaatttgtc tcaccttctt tctgttattt gacgattctg ttagactaga
    13021 tataataaaa aaagtatagt gaaagaagga aaaaaacatg gaaatatccg aaattcgaaa
    13081 tctcctgaca gaaatgcagg aaaaggtcac aagcttcagg aggtctcttt gacttagacc
    13141 agctggaaga agacattgcg gaagcagaag caaaaatggc tgaacctggt ttttgggatg
    13201 atagcgaagc tgctcaaaaa gtgatcagtg aaaacaatgc aaataaagaa acttatgatc
    13261 aattccattc tcttgctgaa gaactggaag aaatcgaatt gatggtggag atgttgcagg
    13321 aagaacctga tgcagtaatg caagaagaag cagaagcacg tatcaatgcg ctagatgaaa
    13381 aaatgaatac gtatgaatta tctatgttgt tagatggtcc ttatgatcga aacaacgcca
    13441 tcgtcgaatt gcatccaggg gctggcggaa cggagtccca agactggggc agcatgcttt
    13501 tacgcatgta tacacgctgg gcagagcagc atgggtatca agtagagaca ctagattatc
    13561 aagcaggcga tgaagcagga atcaagagtg tgaccctact gatcaaagga cacaacgcat
    13621 ttggctatct gaaatcagaa aaaggtgttc atcgccttgt acgtatctcg ccttttgact
    13681 cagcaaaacg tcgtcatact tctttttgtt cggtagatgt gatgccagag cttgatgaaa
    13741 cgatcgatat agaaatcaat ccagatgatt tgaagatcga cacatatcgg gcgagtggtg
    13801 caggtggtca gcacatcaat aagacagaat ccgctgtacg aatcactcac ttgcctacag
    13861 gaacagtagt agcgagtcag gcacaacgtt cacaattgaa aaaccgggaa caagcgatgg
    13921 ggatgctgaa agctaagctt taccaattag aagtggaaaa gaaagaacaa gaagcagctg
    13981 cactgagagg agaacaaaaa gaaatcggct ggggatcaca gatccgttct tatgttttcc
    14041 atccatattc tatggtaaaa gaccatcgga caaacgtaga gacaggaaat gtccaagctg
    14101 ttatggacgg agatcttgat ttatttattg acggctattt gaaaatgcat ctgcaataag
    14161 agccctttgt taaattgaaa taaaattgta agattttgca agcaagttga aacattgcca
    14221 tgctataata gctaaagact gaaaaagata tggagagaac gccatgattg aaatgaagga
    14281 tgtaatgaag aagtactcaa acggtaccac tgctatccgc aatctctcga ttgtcatcga
    14341 acaaggagag tttgtgtatg ttgttggtcc gtctggagct ggtaaatcta cattcatcaa
    14401 attgatgtat cgagaagaaa aagcttcaaa aggaactttg aacgtcgcag gtcatgatct
    14461 aatagccatc aaaaaccgcg aagttcctta tctacgcaga gaaatcggcg tggtgttcca
    14521 agactataaa ttattaccaa gaaaaacagt atatgaaaat gttgcgtacg ccatgcaagt
    14581 tatcggcaga aaacctcggg atatcaaaaa acgagtaatg gaagtgctgg atcttgttgg
    14641 tttgaaacac aaagtccgag tctttcctag cgaattatct ggtggggaac aacaacgtgt
    14701 ttctattgcc cgagcaatcg taaatacacc taaagtactt atcgcggatg aaccgacagg
    14761 gaatctagat ccggaaaact catgggaaat catgaaacta ttagatcgga tcaatgcaca
    14821 aggaacaact gtcgtgatgg ctacacataa cagtacgatc gtaaatacga ttcgacaccg
    14881 agtaattgct atcgaaaacg gccgcattat tcgagaccaa gcggaagggg aatatggata
    14941 cgatgattag aacctttttc cgccatttat tagaaagtat caagagtttg aaacgtaacg
    15001 gctggatgac agtagcttcg gtcagtgcgg taacgatcac gttgacttta gttggtgtgt
    15061 tcatggcggt catcatgaac gcaaccaaac tagcacaaga tatagaagga aacgtggatg
    15121 tatctgtgtt cgtagacatt ggtacaaaag acgaagagat gaaaaaatta gaaaaagaat
    15181 taaatgactt agatcatgtc aaaaaagtcg aattttcaag caaagaacag gaattgaaga
    15241 aaatccaaaa cgaaatgggc gattcttgga aattgtttga aggggataat aacccgttat
    15301 atgatgtata cgtagtaagt gctaccgatc cttcttatac aaaacaagta gcagaaaaag
    15361 cggcagattt gaagaatgtt tcccgttctg attacggtgg ggcttcctca gatcggattt
    15421 tccaaattgc tggtgcagtt cgaacatggg gattagttgc ggcggcattg ctgctgtttg
    15481 tagcaatgtt tctcatttct aacacgattc ggatcacgat tttatctaga cagcgtgaaa
    15541 ttcaaatcat gcgtttagta ggggcaaaaa atggatatat ccgctggcca tttttccttg
    15601 aaggtggttg gatcggctta atcggtgcca ttcttccgat actgatcatt acatttggtt
    15661 acgcatggtt ttaccaattg atcaatccgt cattattacg atctcattat tcattgatcc
    15721 atccagaaaa tatcgtttgg aaaatcaatc ttttgatggt cggtattggt gtgatcattg
    15781 gttcactcgg ctcgatcatc tctatgagac gtttcttgaa attctagtgt cacaagataa
    15841 atataatcag aaagttggac gatttcgttc cagctttctt tttttatcag ctaggtggag
    15901 taaatggggc ggcttttttc tctttacttt aaaaaacaac ttgggtattc ttaagttggt
    15961 agaaaaagag gtgaagccgt gaagattatc gttgacaatt tttttacgat ccttttgatt
    16021 atgaatattt tgctttcctt tattatcgtt tttcgagaaa gaaaggaaac tgcgcaaaca
    16081 tgggcatggc tgctcgtttt gatgttcatt ccagttgtag gatttatttt atatattttt
    16141 ttaggaagag ggatttccaa agagaaaatc tttgatttga agatccaagg gaaaatcggt
    16201 aagaatctag aaatcgaaga agataaacaa gcattgatgc gagggcttta tcctcatccg
    16261 cctactggac aagtagatgt gaaacaattg atctatatgc ttactgtatt tgaaagtacg
    16321 ctttatacga ctaaaaatga aatcacttta tttacagatg gtcgtgaaaa attcgatgcg
    16381 ctgatcgagg atatcaaaca ggcagaagac catatccatt tccaatatta tatttaccgc
    16441 agtgatacat taggtgaaga agttcgagag gcattgacag aggcagcaag acgcggagta
    16501 aaagtcagag tccttcttga tgcatggggt tccacacaag tttcttctag tttttttcag
    16561 aatttgaaaa aagcaggtgg agaagtcgca tttttctttc ctctattcgt tccgtatatc
    16621 aatcctcgaa tcaattatcg caatcatcgt aaaatcgtag tgattgatgg gaaaatcgga
    16681 tatacaggtg ggttcaatgt tggaaacgaa tatctaggat tggtaaagaa atttggttat
    16741 tggcgagaca atcatctgcg tatctatgga gaagcggttt acaccttgca aaaccgtttt
    16801 ttgatggact ggaattctca gcatacaaaa gaagtaggat attcaccgga atttttcccg
    16861 accattcatt ctacaggtga tatggctgtt caaatcgtga caagcggacc agataccgaa
    16921 catgagcaaa tcaaaatgac atatttgaaa atgatttcgt tagccaaacg ggagatattg
    16981 atccaaacgc cgtattacat accggatgga tcgattcatg aggcattgaa gttggctctt
    17041 ctctcaggcg taaaagtcca tatccaaata cctaacaaac cagatcatct tctcgtttac
    17101 tgggcaacgt attctttcgc tgcagagctg atcgagtatg gggcaagaat cgaaacatat
    17161 gaaaatggat ttatccatgc aaagacgatg attatcgatg gcggcatcgt ttccgttggt
    17221 tcagctaata tcgacgttcg ctcatttaga ttggattttg aagtcaatac actgatctat
    17281 gatgaacgaa tggcttctcg cgtaagaaaa gcattctttg aagattcaaa gatttcgacg
    17341 catctcacaa aagaagtgta tgaaaaccga gggatgatga tcaagatgaa agaaggatta
    17401 gcaagactga tttcgccgct cctttaaaat acaaaaagga caggagagcc atcaatagat
    17461 ggaaatctcg cgtccttttt tctcggggcc atttctgaga gtacgtcaga aatggagaaa
    17521 gcgtctcgca tgagcgaaat taactttggg gtatctcttg ttttttgggg aaagcaagaa
    17581 ataagctaat atatgctatt aaaagagaca catttcaaag ccagtatagc atagaaactt
    17641 ttaatgagat agcttttttt gcaattatga gaatttattt tgttatttaa tgattttgat
    17701 tcgttttcat caagtaatga aaatgataga ataaacatac tttaggatta aaggagattt
    17761 ctaatgaaaa aactaaaatt attcttacca ataataggat tagctattct tttatcaggc
    17821 tgttcaaagt ggattgatcg aggagaatcc attactgcag ttggctcttc tgccttacag
    17881 cctttagtag aaacggttgc tgaaacctac caggcagaaa atcctggaaa attcatcaat
    17941 gtacaagggg gcggaagcgg aactgggttg tctcaagtac agtcaggagc agtcgatatc
    18001 ggaaattctg atttgttcgc ggaagaaaaa tcagggattg atgcttcggc attagtcgat
    18061 catcgagtag cagttgttgg aatcactcct attgtgaaca aggatattgg cgtaaaagat
    18121 atcagtatgg aaaacctgaa aaaattattt acgggcgaga tcaaaaactg gagtgaagtc
    18181 ggtgggaaag atcagccagt agttatattg aaccgtgcat caggaagcgg gacccgtagt
    18241 acgtttgaaa aatgggtatt ggacggagaa acagctgttc aggcacaaga acaagattca
    18301 agcggaatgg tgcgccaaat cgttgcggac acacccggag caatcagcta tgttgctttt
    18361 tcttacgttt ccgatgatgt ggatacgttg aacgttaacg gtgtggaacc aacagatgaa
    18421 aatgtgacaa cgaacaaatg gacgatttgg gcatatgagc atatgtacac aagagaccat
    18481 ccaaaaaaac tgacaaaaga atttttagac tatatcttat ctgatgaagt acaagataat
    18541 attgtcggag agctaggcta tatccctgtt tcacatatga aagttgaacg tgattggcaa
    18601 ggaaacattg ttaaagagta atctttacac aaaatttaca aacttagcta aacttttaca
    18661 tgttattaaa accaaattga tgttcaattt gtacactgag tgagtatgtt aaacctgatt
    18721 aagggggagt catctgtgga agatgtgaaa aaagtattat taacaaaatc aaaacgttca
    18781 aaaatggaac agcgcggaaa attcattagt tttttgtgta tcgcgctgat cgtgttggtt
    18841 gtattatcta ttttttattt tgtcgctagt aaaggattgg ccaccttttt tactgataag
    18901 atcaatgtat ttgattttct gtttggtaca gattggaatc caagccaaat aggagcaaat
    18961 gggaaaccca tggtaggggc attgcctatg atcacaggtt catttattgt gaccttttta
    19021 tctgctattg ttgccacacc atttgctatt ggagcagcag tgttcatgac ggagatttct
    19081 cctaaaaacg gaacgaagat cttgcaaccg gtcatcgagc tacttgtagg tattccgtct
    19141 gtcgtttacg gatttatcgg tttatctgtt atcgtgcctt ttgtcagaag tatctttggc
    19201 gggacagggt tcggtatctt agctggtaca tttgtgttat tcgttatgat cttaccgact
    19261 gtcacaacga tgactgtgga tgcattaaag gcagtgccgc gacactatcg ggaagcttca
    19321 ttagcgctag gtgccacaag atggcaaaca atctacaaag tggtgctgcg agcagcagtg
    19381 cccggaattt taacagcagt cgtttttggt atggctcgtg cgtttggcga agccttagct
    19441 atccaaatgg ttattggtaa tgcggcatta atgccaacag gacttactac tcctgcttct
    19501 acattgacgt ccatcttgac aatgggaatc ggaaatacaa tcatgggaac agttgaaaat
    19561 aatgtgttat ggtcattagc gctgatcttg ctattgatgt cattattctt taatatcgtg
    19621 atccgtatga ttgggaagaa aggagagctg aaataatgaa cgcaaaacgc tcagataaaa
    19681 ttgctacagg tgttctatat gtggtttcag ggatcatcgt attgatctta gcagctttat
    19741 tactttatat cttagttcga ggaataccgc acatttcatg gtcattcttg actgaacctt
    19801 ccaaagccta ccaagaaggc ggtgggatcg ggattcaatt atttaactcg atttatttgc
    19861 ttttgattac catgatcatc agttttccta tctctttagg tgcgggtatc tacctttcgg
    19921 aatatgccgg aaaaaactgg ctgacagacg tgatccgtac gtctatcgaa atcttgagct
    19981 ctttgccttc tgttgttgtc ggtttattcg gtttcttggt tttcgttatt caattccagt
    20041 atggtttctc aatcatttct ggtgcattag ctttgaccgt attcaacttg cctcttttga
    20101 ctcgaacggt cgaggaatct ttacaagcgg tccattacac acaacgagaa gctggattgg
    20161 cgttaggttt gtcacgctgg gaaacagtga taaaagtggt tgttccagaa gcgacaccag
    20221 gaattttgac tggtgtgatc ctaagttcag gaagaatttt tggtgaagct gccgcattga
    20281 tttatacagc tggtcaaagc gcgccagcat tagattttac gaactggaat ccattaagtg
    20341 tttctagtcc gatcagcatt ttccgccaag ctgaaacact ggctgtccat atttggaaaa
    20401 tcaacacaga aggtacgatg cctgatggtg tttctgtttc ggctggcgct tctgccgtgt
    20461 tgatcattgc tgttttactg ttcaacttcg gcgctcgtgc gattggcaaa cgattatacc
    20521 gaaaaatgac ttcagcctaa ggagaagaag taaaatgaaa aaatataatt ggaatgaaac
    20581 aaatgtcatt accatgccaa aagataagga agttgcatta tatacgaatg atctgcatgt
    20641 ttggtatggc gacaatgaag caatcaaggg agtggatcta gaattcgaaa aaaataagat
    20701 cactgcattg attggtccct ctggctgcgg aaaatccacg tatcttcgtt ctttgaatcg
    20761 tatgaatgat gggattgcta atacgaaaat tactggaaag attatgtata aagacgtgga
    20821 tatcaatgcg cctcaagtgg atgtttatga aatgcgtaaa cggatcggca tggtgttcca
    20881 acgccccaac ccttttagca aatcgattta tgacaatata acttttgctt tgaaacagca
    20941 tggtgaaaaa aacaaaaaga aattagatga aatcgttgaa acaagcttga aacaagcagc
    21001 tttgtgggat caagtaaaag atagcttgga taaaagtgcc ttggctttat ctggcggaca
    21061 acagcaacgt ttgtgtatcg ctcgtgcaat cgcgatgaaa cccgatatct tgctgttaga
    21121 cgagccggca agtgccttgg atcctatttc gacaggaacg gttgaagaaa cacttgtaaa
    21181 cttgaaagaa gactacacga tcattatcgt gacgcataat atgcaacaag cagctcgtat
    21241 cagcgactat actgcctttt tctatctagg aaaagtaatg gaatatgaca aaacaaacaa
    21301 gatatttacc agaccaaaaa ttcaggcaac cgaagattat gtgtccggtc attttggtta
    21361 agacggaggc ctcaacatga caaaagaaat tatctcatcc aaagatcttc atctgtatta
    21421 tggaaagaaa gaagcactaa agggaattga tctaagtttc aatcaaggtg aaatcacagc
    21481 gatgatcggt ccttctggtt gcgggaaatc tacctatcta cgttcgttga atcgaatgaa
    21541 cgatctgatt ccaaatgtaa cgatcaccgg aagtgtttta tataaagaca aagacatcta
    21601 tggtccgaaa acagatgttg ttgaactaag aaaagaaatc ggcatggtgt tccaacagcc
    21661 aaatccattt cctttttcta tttatgaaaa tgtcatctat ggattgaagt taaaaggtga
    21721 aaaagataaa aaagtccttg atcaagtagt ggaagaaagt ctgaaggcag cgtccgtttg
    21781 ggaggatgtg aaagataaac tgcataaaag tgccttgtct ttatccggtg ggcagcagca
    21841 gcgtgtatgt atcgcacgtg ttttggcagt gaatcccgaa atcatcttat tggacgaacc
    21901 aacaagtgca cttgaccctg tttctactgg gaaaatcgaa agtatgctgt tagaattaag
    21961 agagcggtat acgatgatca tcgtgaccca taatatgtct caagcttcac gtatttcaga
    22021 taagacggca ttctttttag atggtcactt gatcgaatac aacgatacga agaagatatt
    22081 catgaatcca gaaaaacaag agacagaaga ttatatttca ggtcgatttg gataaggagg
    22141 aaacggaatg ctacgtacac aatttgaaga agaattgctg aatctccata atcagttcta
    22201 cgaaatgggg atgatggtga gcagcgctgt gcacaagtca gttcgtgcat acatcaaaca
    22261 cgataaaatc ttagctcaag aagttatcga aaacgatata aatatcaata atatggaaac
    22321 gcgtttggag aagaaaagtt ttgaaatgat tgcattacaa cagccagtta caactgactt
    22381 gcgtatgatt atcacggtca tgaaagctag ctctgattta gaacgtatgg ccgatcatgc
    22441 ggtgtccgtt gctaaatcca caatccgcgt aaaaggacag acgagaatac cggaaatcga
    22501 aaaagagatc tccgatatgt ccgattacgt gaaaaaaatg gtggacaacg tcctagttgc
    22561 gtatgtcaaa acagacgaaa aagatgcgcg tacaatcgcc aatatggatc aaagagtcaa
    22621 tgactatttc aatcgaatct ataatgcaac catcaaaaac atgcaagaaa atcctgaaac
    22681 agtcatcagt ggcacagact atttgaatgt tgccagctat ctagaacgaa tcggagacta
    22741 tgtgacaaac atctgtgaat ggatcgtcta cttggctaca gggaaaatca ctgaattaaa
    22801 tacgaaccac aatgagattt tctaaactga agttgtgatt ctgctgttta tttaggaaaa
    22861 gaggttgtga aatcagcgtt ttgtcctgag tattagagaa acaaacggta aaatagttcc
    22921 tcatattttg ccgttttttg agctaatacc gaaggtcagc tgattgaaca ccgtttattc
    22981 tggaaaaggg tttgtgacaa aacatgtcac aaaccctttt cattattcca atagaaagga
    23041 taaagaatgc aaaaaaagat tagcatcatc atcactctat tttttatttg tatacttgct
    23101 tcatggttta ttcagcgtca agctgctcta tatgacgtac ctgtcatcga gatcgaaaga
    23161 gtgagaatag aagaggatca acaattgatt acaggaagat tgatgaatac ggaccaaaaa
    23221 ggagaagtga tttctttaac ggctccttat caaaaaaatc aaatcgaaac agcaaatata
    23281 acaacaagac aaatctatct tgtccaaatg catggagatg aatggcaact agtagcaaaa
    23341 aaaaatgatg gctggctttt tttctttaca agtgtcttca ttggcaccat gttgcttatt
    23401 ggtggaaaac agggcgtatt tgctttagga agtctcatca tcaacatcgt tttactttta
    23461 cttttgttgc tcctctttac caaaacagaa ggtatttctc tcttaaatat agcaatctta
    23521 tttgtcgtta tagggataat cgttagtata ttggcgttag acggatggaa tagaagcagt
    23581 ataatcaaaa tttcggctgc agttacaagt gtttttttag cattttttat ttgtcttttg
    23641 acaatgaatc attggaaaga tcaaggactg cgctatgaag aactgaatta tctgacacga
    23701 ccgtatcgtt ctgtttttct ttccagcgtg ctgattggga cagctggagg gacactcgat
    23761 accgtgatca cagtcattgc gacattagaa gaattgacca agcaacaacc gaaaatatca
    23821 gcgaaacagt tatggctttc aggaaggcaa gtgggccgag atgtgattgg ttcgatggct
    23881 aatatccttt tattcgtcta tatcagcgga gcgattccat cgcttctgtt atatttagga
    23941 aatcaatggt ctttcaaaga aacggtagaa cgacatttat cattagaact tttacgcgtc
    24001 attgctggca gcttaggaat agtattatca atccccatcg cggtcctctt ttttctcgct
    24061 gtaaggaggt taaaaaaatg aatgtactta ttatacttgt tgggattttt gcaatcagtg
    24121 tgctatttgt cggtggtacg caaggtatgt atattctttt aggattattc attaatttag
    24181 gaatcttttt cctattatta ttcggctatc atcagaagtg gccgatattg gtattgtcga
    24241 ttatagggtt cctattgatc gctgtggtta tcttattttt tatcaatggt tataatctga
    24301 aaatgcgagc cgcttttgcc agtatactga tctttttgtt ttgtttccta ctattgattc
    24361 ctatcactga ttttttagcg atccaaggct ttacttcgat agaactagaa gaactatctg
    24421 gcttagataa gacgttagca attgatttta gactacttac gaggtcgttg cttctgatca
    24481 gtcttagcgg agctgtttta gatgcgagtg tagcgatttc tagtggaaca ttcgaagtat
    24541 atcaggcaaa tccgcaaatg tcttttaacc aattgcgcca tgctagcttt gccattgcta
    24601 aaaaaatatt ggcatcaaca gtgatgacgc ttctttttgc gtttatgggg agttctttag
    24661 cgttgattct ttggtttatt gacttagaca tttcttttca gcaaataatc aatgaaaaat
    24721 catttgtctt ggaatatacg atggctattc ttaccacact ggccgcacta cttgtcctgc
    24781 ccttaacttg cgtgactaca gcatatctct ttacaaaaaa gaaagaacat ctcaagatgg
    24841 agtaaaagtt acaacttaag tcctatgaca tttttactga atcagtgcat aatatggatg
    24901 taaggaaaag cttgcaaaca attttgctta aggaggcaat ttccatgaaa gaaagagaac
    24961 gtattttaga cttagtgaaa aaaggaatcc tttcgactga agaaggctta gatttattag
    25021 aaagcatggc aactgaaaaa gacgaaaaac aaatcaaaaa agaagcagac aaggtaactg
    25081 ctagtcataa agaaaaagat caggcaagtc aattgatcga tgaacttgaa aacggtgaag
    25141 aaaaaatttc tgagccagtt gatccaaaag aaaaagaaag acaagatcaa gaaaacttag
    25201 aaaagatctt agatgaacta gcaactgaag cgaataaaac ttcggcgcgt ctagacgaag
    25261 taaatgctca attcgctgac aataaggcag cacgtaatga gaaacaggaa gcattgatgc
    25321 agctgaatac aaaagaagaa ttaggcgagt tgacagaaga agaattagca cagcgtcaaa
    25381 cgttagaagc agaaatcaaa gagttagaag caaccggcga tacactatta gaagaacaat
    25441 tgaagctgga agcagaatta aaagatatcc gcaagaatca atggtctgag aaaaaagaaa
    25501 catttacgga taagtttgaa ttgccagatg attggaaaga tcaagcgaca gatacgctga
    25561 atcaagtagg cgaaaaaatg tcagaagctg gatcacagtt aggcaaattc ttgaagaaaa
    25621 cattccaaac tgtttcagaa acagtaaatg ataacatgga atggaaagat gtcagcttac
    25681 gcgtccctgg aattgcgaca acgaaatttg aacacgaatt ttattatgaa gctcctgcag
    25741 caagtatcct tgatatcaag gcagcaaacg gaaacgtgac gttaaaaacg tgggacagtg
    25801 atgatgtgaa agtggaagcc aagatcaaac tttacggaaa aatgggtgct gaaccatttg
    25861 aagcattctc tgaacggagc cagatcgagg tcaatgaaga tcatatctcc ttccaaattc
    25921 caaataagcg tgtgcgtgca gaccttgttt tctacttgcc taaacgagta tacgaccatg
    25981 cagctatcaa acttctaaat ggcaacatca tgattgagac attggaagct aaagatattt
    26041 atacaaaatc tacgaatggg aacatcatcg tgaaccaatt aactgcaacg atgctggaag
    26101 tagaaggtgt aaacggcaat atcgatattc gaaacgggaa catcttagat tctattatag
    26161 aaacagtaaa cggtacagtg acatttggtg ccacaccaga aaacctttct gtgtctttag
    26221 taaatggtga tgtacgtctt acgattaaag aagataattt gaaaaaagta gaagcaagtt
    26281 cagtgaacgg gaatgtcaaa gttgctttac cagatagcat cggtttggaa ggacacgcta
    26341 aaacaagtct tggaagcatt aacagccgtc tgtccaatta cgaagtagtt cgtgagaaaa
    26401 aagaacggac aaaccaaatg ttgcaattcc gtcgtgtttc agatgatgag atcgcacaag
    26461 tgcagctttc aacgacaaca ggaagtatct atttaaaaga tacagataaa taaagaggtt
    26521 gtgaaagaga tgttcagcct tgagcaataa gaagcgattt tttaaaatag cttcccctat
    26581 tttaaaaaaa tgtgccttat tgccgatagg ctatctcttg aacaccgatt aagaagaagg
    26641 ttgtcacaac gattttcttc atttaaaata gtagaacaga ggtgagacat gatgaaaaaa
    26701 aagttgacaa aatccaataa aaatgttgtt ttgacaggta ctttagcagg gattggggaa
    26761 tatatcggga ttgatcccac tgttatccgt gtgctttatg tattcttaag cttagttgct
    26821 tttggaagtc caatcatttt atacatttta ttagctttga ttatcccatc tgcgcgttct
    26881 gcacgaacaa actatggaca taacaatgca tattatgaaa aaaacaatta tcgtgaacaa
    26941 acaaaaaaac gcaaagaagc ggaaaaaatc gatgatgacg aatggagtga cttttaatgt
    27001 cgtactttca gcgtattttg gttaatacgt taacattcat atcgttggct gttctttttc
    27061 ctgcgatggt ccatgtgagc agtatttgga cggccgtcct tgccagcttc gtattatctg
    27121 tgttgaacat gtttgtcaaa ccgattttga cgatcctgtc cctgcccttt acattagtca
    27181 cattgggatt gtttacgttt gttatcaatg cagcgattct taaaatgact tccttttttg
    27241 taggagcaga acattttagt ttttcttcat ttggtgcagc gttattcgta gctatcttga
    27301 tgtcacttgt caatgtaatt gttagtgaac ataacagcag aaaataaaaa aagctgtgcc
    27361 gagatctcgg cgcagctttt t
//
LOCUS       DAIGOK010000026        26595 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.026, whole genome shotgun sequence.
ACCESSION   DAIGOK010000026 DAIGOK010000000
VERSION     DAIGOK010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26595
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.026"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..1412
                     /locus_tag="NOA01_000878"
     CDS             <1..1412
                     /locus_tag="NOA01_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCI0632093.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
     gene            1546..2889
                     /locus_tag="NOA01_000879"
     CDS             1546..2889
                     /locus_tag="NOA01_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCI0632094.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            3001..4452
                     /locus_tag="NOA01_000880"
     CDS             3001..4452
                     /locus_tag="NOA01_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCI0632095.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSTESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            4801..5433
                     /locus_tag="NOA01_000881"
     CDS             4801..5433
                     /locus_tag="NOA01_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HCI0632096.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            6021..7460
                     /locus_tag="NOA01_000882"
     CDS             6021..7460
                     /locus_tag="NOA01_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCI0632097.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            7755..7973
                     /locus_tag="NOA01_000883"
                     /pseudo
     CDS             7755..7973
                     /locus_tag="NOA01_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            8222..8437
                     /locus_tag="NOA01_000884"
     CDS             8222..8437
                     /locus_tag="NOA01_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632098.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(8654..8910)
                     /locus_tag="NOA01_000885"
                     /pseudo
     CDS             complement(8654..8910)
                     /locus_tag="NOA01_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(9097..10008)
                     /locus_tag="NOA01_000886"
     CDS             complement(9097..10008)
                     /locus_tag="NOA01_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCI0632099.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(10027..11031)
                     /locus_tag="NOA01_000887"
     CDS             complement(10027..11031)
                     /locus_tag="NOA01_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCI0632100.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNRSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(11028..13151)
                     /locus_tag="NOA01_000888"
     CDS             complement(11028..13151)
                     /locus_tag="NOA01_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCI0632101.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(13156..15603)
                     /locus_tag="NOA01_000889"
     CDS             complement(13156..15603)
                     /locus_tag="NOA01_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCI0632102.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(15590..15979)
                     /locus_tag="NOA01_000890"
     CDS             complement(15590..15979)
                     /locus_tag="NOA01_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCI0632103.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(16039..16542)
                     /locus_tag="NOA01_000891"
     CDS             complement(16039..16542)
                     /locus_tag="NOA01_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCI0632104.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(16555..16776)
                     /locus_tag="NOA01_000892"
     CDS             complement(16555..16776)
                     /locus_tag="NOA01_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632105.1"
                     /translation="MNFGQNLYQWFLTNAQSLVLLAIVVIGLFLGFKREFSKLIGFLV
                     IALIAVGLVFNASGVKDVLLSLFNRIVGA"
     gene            complement(16781..16918)
                     /locus_tag="NOA01_000893"
     CDS             complement(16781..16918)
                     /locus_tag="NOA01_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HCI0632106.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(16915..18099)
                     /locus_tag="NOA01_000894"
     CDS             complement(16915..18099)
                     /locus_tag="NOA01_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCI0632107.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(18358..18648)
                     /locus_tag="NOA01_000895"
     CDS             complement(18358..18648)
                     /locus_tag="NOA01_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632108.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            complement(18638..19780)
                     /gene="dcm"
                     /locus_tag="NOA01_000896"
                     /pseudo
     CDS             complement(18638..19780)
                     /gene="dcm"
                     /locus_tag="NOA01_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            complement(19856..20020)
                     /locus_tag="NOA01_000897"
     CDS             complement(19856..20020)
                     /locus_tag="NOA01_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632109.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            complement(20013..20687)
                     /locus_tag="NOA01_000898"
     CDS             complement(20013..20687)
                     /locus_tag="NOA01_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCI0632110.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            complement(20765..22105)
                     /locus_tag="NOA01_000899"
     CDS             complement(20765..22105)
                     /locus_tag="NOA01_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCI0632111.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            complement(22188..22523)
                     /locus_tag="NOA01_000900"
     CDS             complement(22188..22523)
                     /locus_tag="NOA01_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HCI0632112.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            complement(22533..22907)
                     /locus_tag="NOA01_000901"
     CDS             complement(22533..22907)
                     /locus_tag="NOA01_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCI0632113.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            complement(22920..23234)
                     /locus_tag="NOA01_000902"
     CDS             complement(22920..23234)
                     /locus_tag="NOA01_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCI0632114.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(23347..26574)
                     /gene="fss3"
                     /locus_tag="NOA01_000903"
     CDS             complement(23347..26574)
                     /gene="fss3"
                     /locus_tag="NOA01_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HCI0632115.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVALVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
BASE COUNT         8106 a         5354 c         4753 g         8382 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa tcagaaatta gttacttgga atgtgggaca
     1681 tcgttattta taaaaggaga agtaagtaat ttagaagatc ttttttatca aaatagaatg
     1741 acactaaatt ctgagataaa aaagttattt ctagagacag aagttgacaa aattgatatt
     1801 caaaataaaa agattagttg tgtaaaaaaa gatggtacaa gattctctca agaatatgac
     1861 aaagttatat tagctacagg ttcaaagcaa attatgctag atataccggg gaataattta
     1921 gctaatattt tttcgataaa gaattttcca aatgcaaaag agatttttaa agctttggat
     1981 gatagtacta ttaaaaaagt aggaattatt ggagcaggat atattggagt tgaaattgct
     2041 gaagccataa gaaaaagagg gaaagaagta tatcttttcg atgttgcaga tagagtgttg
     2101 tcgacttatt acgaccgtag tttttcagat aaagttgaag aaatactatc aaaaaatgga
     2161 atacatcttt gtctatcaga aagtcctaaa aaatatttag gaagaaaaaa gattgaaaaa
     2221 atagtcacta caagtggtac ccaatactct atggatatgg tcgtacaagc tataggattt
     2281 ctatctaata gtccaattgg ggaaaaggaa ctcttgagag atgttagtgg agcatatcta
     2341 actaatgaat accagcaaac aaatataaaa gatatatatg ctattgggga ttgtgctaca
     2401 acttttttta cgtccattaa tagaagtagt gttgattttt ctatttctaa tgctctaaga
     2461 acagcttata ttgcaactca acatattaac aatcaagatt ttacaccttc tggttcccaa
     2521 tttacaaatg gcttttctat atttaattac aatttttacg cagcaggttt gaatttaaaa
     2581 acagcacgat tattaagtga gtctatagat tatatagaaa tagaagattt cattagacct
     2641 gcttttttga gaaaaaatag tcgtgtaaaa ttaagaatta gctttgaaaa aaatacaaag
     2701 agaatagtag gtgtacaact ttgtagtcaa gaagatttat ctggactatt atcaatgttt
     2761 tctcttgcta tagaagaaca aattacatta gacaaattga aaatgttaga ttatttattt
     2821 catcctagtt ttagccaacc ctataatttt attttaaagg cagtaacagg attaaacatt
     2881 gaaacataat cgttactaaa tgaaatcaag aaatatactg tattactcaa ttcattgaaa
     2941 atatttatca taatttttaa tagaaaacac tattaaagag aaaaaagaga ggtaaatcaa
     3001 atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca
     3061 acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc
     3121 gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact
     3181 tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa
     3241 agtagtacga cttcaaatgc cgagtcttca acaacttcat caagtgaaag tagtacgact
     3301 tcaaatgccg agtcttcaac aacttcgtca agtgaaagta gtacgatttc aagtaccgaa
     3361 tcttcaacaa cttcaagtag tacaagtgaa ataacttctt cgaaagtgac aaaaccaaac
     3421 catatagaat cttcaaaaaa ggaagcgcca aaaacggaag gacaaacaaa aactttttcg
     3481 acaccacagc aaatagaatc aacagaatct ttccaatcac aaccaatact atctggtaat
     3541 gaagaaattc attttgacaa aaatgaatct gtagaaaatt ttattatgaa aattggtgag
     3601 tctgcgagaa aaattggtca gaaatatgat ttatatgctt ctgttatgat tgcacaagcg
     3661 atcttagaat ctgccagtgg tcaaagccag ttagctcagg caccgaatta caatttattt
     3721 gggataaaag gaacttataa tggaaacttt gtcattatgg taacaaacga agatttgggt
     3781 aatggaacgc tttatacgac ccaatcaaaa tttagagtgt atgaaaacta tgaggaaagc
     3841 tttgaagatt atgccaaatt attaactaag ggaattagtg gaaataaaga tttttatgca
     3901 ggagcattaa aagctaatag taaaacatat cgagaagcaa ctaaattcct tacaggacgg
     3961 tatgcaacag atacacaata ctatttgaag ctaaatgaat taattaaaac ctacgattta
     4021 accaactatg ataaagaagt taaaggtcca caaatgagta agactggata ccaagtgcct
     4081 attcataatt attcaataac cagtttgttt ggaaatcgtg gaaatgaatt tcaccgagga
     4141 cttgatatgg cagctacaca gggtgagccg attcatgcag ttaaagctgg taaggttgtt
     4201 aaagcagagt ttcactactc ttggggaaat tacgtggtga tacaacacga agatggatca
     4261 accgcgttat atgctcacca gcaacaatac ctagtaaaaa ctggagataa tgttttgcaa
     4321 ggacaaataa ttggctatgt aggaagtact gggaatagta ctggcccaca ccttcatttg
     4381 gaaatttgca aagatagtag tctttctcag agcatgttag ttgatccgaa aaccttatta
     4441 ttcggagact aaaaaaggta atcaatctga caaagtaaca agaaaatggt tggattctta
     4501 gacctttgat caggatattg agactggaat tgatatctcg attacgatgg gtttgacaaa
     4561 taaaacaagt ccattatcga atatcgagtg tattattgcc gtctttatgc tcatattttg
     4621 agcaggtttc actagatgaa aacaatttat ctatcttgag gatttcgtgt acgtatcgtt
     4681 cagtcttgta ttgtaaatta gtcataaaat cagactaaga aaaattggaa atattttttt
     4741 ctgatttatt aaaagttaat tgaaattata tggtacttct ttcttgggag ggtaggtgca
     4801 gtgaaggaaa gatatggtca ccttactgtt gttgaggaga ttactcataa tggatataaa
     4861 aaatatcgtt gcctatgtga ctgtgggaat gaaacgattg tatatcgatc aaacttacta
     4921 tcaggtagaa ccaaaagttg tggatgtgga aattataaaa ataggcataa atataaaaat
     4981 ttgacaaata aaacttttgg taagttagtt gcaatcaagc caactaaact gcgaagtgaa
     5041 ggaactgttg tttgggaatg tcaatgtctg tgtgggagaa aaaagttagt atcggcaaag
     5101 cgacttctaa aaggtgatgt aaaaagctgt ggatgtttga aatcaaaaaa gcatcacatt
     5161 gaaaataaaa aattcggatt acttactgca aagtatcctt tggacaataa ctgttcatgg
     5221 aaaacaaaat gggtttgtca ttgtgagtgt ggcagtatgt gtgagataag ttatagtaac
     5281 ttattttttg gacatactca aagttgtgga tgtttaaaaa ggcaagaaac aaggacatgt
     5341 gttgaaggga cagtaataga gtgtctaacg tcaaaattac cgaaaaataa tacaagtggt
     5401 gttaagggag tttatcgttc taaagagaaa tagattgcgt atataacttt aaaaaaaaag
     5461 aggtactatt taggatcgta tgattctatt aagagtgcag ctcaagctag aaaagatgct
     5521 gaaaataaat tgtttcaacc tttgattgac aaatatcgag gtaatcatta attattgtgg
     5581 ataataatta acagtgatat ataaacatct ataatatatt attcatacta ttgatattta
     5641 gtataatcct atggaattga atatcatagc aatgtatttg aaaagcaaat tggaaaacaa
     5701 ataggtgcaa caccataaat atgaaaaaat tgggaaaaag tagaatggaa atttgcttac
     5761 gtctcacgag atagctcaca ctattaatct gaagaacaat gaagtcgact tttagaaatt
     5821 gctagaagaa agtaaagcga attatcacta gttactatag agtaagttca aataaagcat
     5881 ctccaactta catggttaca tgaggatatc aaatcgaaat tactcaagct tcctatggct
     5941 agagtcgcga gaagttgttg ggcagtagac ataggttata ttgttcttag ctacacgatc
     6001 attagaaagg aaatcctatt atgtatagtt atcaagaaga tgcaaaagtc caaaaaaatc
     6061 gctggtggat tttgattgct atatctatgt ttaccttcat gtccacattg gatggcagta
     6121 ttgtcaatat tgcactacct accatctcag atgatatggg cataccaatg aatcaagcgg
     6181 aatgggtagt gtccgtttat ctgatgattg tctgttcttg tttgttgctg tttggtaaaa
     6241 ttggcgatag ccgtgggaaa atcaaagtat ttcggattgg tacgctgatc tttgtcttgg
     6301 gatcgctgtt gtgtgggttc aatcattcgc taggtatttt attgttcgca cgggtggtac
     6361 aagccattgg tgctagtatg actatggcta caaatgccgg aatcatcacg gaagtctttc
     6421 ccatacatga acgtggtcgg gcattgggag caataggtgc gtttgtttca ttaggctcga
     6481 ttgctggacc aggtcttggc ggcttgattt tgtcccaatt ttcttgggcg tacatttttt
     6541 ggatcaatgt accggtggga atcgtaacga tgttgattgc tgaaaaattc ttaccaaaag
     6601 atatcaccat gagcggccat acaatcgatg gagggggctt tgctgccatt gcggctacga
     6661 ttatcagctt ttttggagca atctttttgg ggcaagaaag tggctttttc catccagtcc
     6721 cccttgtact gttcgtaatt tcagtgattg cctttgtcag ctttttaaaa gttgaaaaga
     6781 aagcagcctc tccattgatt tctttcggta tttttaagaa caagattttt accatgagct
     6841 tgattacagc cttgttgatt ttttcttcca acttctttgt caatgtagtg attccttttt
     6901 atctgcaaaa tgctcgcggg ttgagtccta gctatgctgg gctgttgatg atggtctttc
     6961 ctttgatgat ggtcgtcggg gcgcctttaa gcggctacct gaccgaccgg gttggcccgg
     7021 agattttggt attagtaggc ttgggcttat tgtcggtaac ccaggtcatg tatatgtttt
     7081 tgtcagtgga ctctcctttg tggctataca ttgtcgctac tgccatcatg ggtgccggca
     7141 atgccttgtt ccagtccccc aataatacga tggttatgag ctcggtcact atcgaaaact
     7201 tgggactggc aggctcgatg aattcctttg cccgaaattt ggggatggtc agcgggattt
     7261 cgctttctac gacggtctta tatcaagcga tgtctgctaa gatgggccag caagtaacta
     7321 actatatcgc aaatcggccg gatgtctttt tatatggaat gaagattacc tttttaggca
     7381 gctttgtttt gtgttttgtt gcctttttcc tgactatctg gcgtatcaaa aaacaaggag
     7441 gtattcagaa aaatgtctga aaaaaccact gttgaagaat ttttggaggc ctgcgtatgg
     7501 gtgagtggga tttccatgga tgatggaaga tgaagtgaac ttctaaaagc cgaaaaactc
     7561 aagtattatc ttcaaaatac tagtgctcaa tgagatgctt gatttgatca caagaagact
     7621 gcgaaagccg ctggcaaccg caagctaggt atcccaccga tggactacgt tttagaggct
     7681 actggttatg aatacggagc caacgctctc atctgcataa gaatacatca cccagattat
     7741 gtaaagattt tcgatacttc tgtcaaaaaa tcattaagat agataaatga tttccgtcta
     7801 gtcaaattgg ttcagaatgt agacacacag atggtaagaa gtcactcaca attcgagaat
     7861 ggacttgtcc tatttgccgt atacatcata atcgaaacgt caacgatagt atcaatattt
     7921 tgaccaaggg ctatgaatac aagcattgcc ttagaaaaca tcaagaacct taggaacaac
     7981 ggggatagct tggttaataa gagataccgc tgtttaggct tgcaagtata ctcttttatc
     8041 aagaattccg tggctttagt catgagaggc ttaatactat gaaaattttc tttagcattt
     8101 ttgatttagg ttccttttct aattataata agatgaccac tacagtaacc tactgaaata
     8161 tataaacagc cactgtagta ccattttcaa agtagtctat gagaggagaa tcttcattct
     8221 tatgaacgct ggaattgcgg atatgaattt aataaaaaaa acccttaatg attttacttc
     8281 caattcaata tcaaaaggta ctggtatcaa tttaagcaca ataaaaaagc tgaaaagtgg
     8341 tgaaagatca gtggaaaagc tgaatctttt agatgctata aaaataacgg aatttgctat
     8401 gaaaaatgga aaagctgaga ttgaaatatg gagataatta tcgtctagtg aattacttgc
     8461 cacttagctg aatttaacga tccttggtgc ttgaaggggg agattcttgg gaatagagca
     8521 tacttgcaag cctatgtctt tactaacaac gatatctctt attaaccaag ctatccccgt
     8581 agttctaagg cttttacttt tatctaagca ataatcgtat tcaaaggaaa tttttgaccc
     8641 ttagaaagtc tcattttatc agacgtgagc ttaggcattg ctgaattttg ttaattcggt
     8701 tttaggttgt gctttgagca taatatgaaa gtattcttta tcacgatttc attcatctaa
     8761 ggtaatgtga tgtgattctg aaattctttc aaaggcagtt tttgcataag ctgacattac
     8821 atcatcaatg acatgtttac aatgtttcac tactaaaata agggggtaac caagaagaaa
     8881 aactgaatga ttatttgtat ctaattccat ggtatatcaa ccatttccta tatagactga
     8941 ttatgacatg gattaaaata aaaaggcact atgtcttaat tgtcggtttt cgagttaccg
     9001 aatggcattc atcacccacc tatagaggat gggcgacttc tgcctgaaat aagttacact
     9061 acttataact tctaataaaa ttagattgag tcattattat ttaacatcaa cgattttcca
     9121 gtttccattt ttttgtaata ctaatttgta ctgaaagtag ctagtggttt ttgatacatt
     9181 atcaaggtat tttatctttg tactcactag aaaatttttt ccgttttgta gataaactgg
     9241 attctctaac tccgaaaact ttaagtcaat attgattggt cgcatcgcac cattttcaac
     9301 ataatactcc aattcttttt cagaagctga tggataaagt ttaaaaaatg tttctaaaaa
     9361 atcggtgatt tcttccgttg ttttggcatc aatttctgaa tcgttttcta cttgttttgc
     9421 ttcatatttt gccttgtctg gctttccaat cattgttggc aaactagtta ctaaatgatt
     9481 cctgttttca tcttcaaaga ttgtcactcg atatgcagaa gaaattgatt gcgtcttttt
     9541 tccctctgta atatgttgct ccactaaaaa tgaaacgaca aattcttccg agtgtttttc
     9601 aacatctaga atttttacag attgaacttc ggaactcgtg ggaatatctg ctcgaaccat
     9661 atcttggatt aaagcgagac cgttttctgt aaggtactgt tcgagattac tcattctctt
     9721 ttcaattgct tctttgttat ttttccatga aaaatattct ttcgcaaaat cctctgtaaa
     9781 actttcaacg ccagaagtat tcactacctt ctcttttata accacttttt catgagttgt
     9841 atgttgatca atggcggtga aatttttata aacgccaaaa gcaagactac aagacaataa
     9901 aagccaaagg gcaagcacca tttttcgatg cttacccacg gatacaactc gttctttttt
     9961 cttttttggg ctcttttcct tttgattccg ttcaattttt atcttcattc tttttcctct
    10021 tttctcttat ttgtatcggc cagctccgat aagatgctgc cgccaatagt tttccgttag
    10081 attcgtccac cccactggat ctcctgcatg aaacatgcga ttctcccctg cgtaaatgcc
    10141 cacatgggtc acataagtac cggcatcata ggttgaatga aagaaaacta aatcacctgg
    10201 tttggcatct tttagatcaa tatgagtcat ggcatcatat tgatcctgag ccacccgagg
    10261 aagtttcagg tcaactttgg cataacacca ttgggtcaag ccactacaat caaaccccgt
    10321 ttctggactt gagccaccaa acacataggc cgttccttca tacttaaatg cctcatcaaa
    10381 aatttcttgg actattttgt catcaaactg agttgtagtt aggtattgct tcactaataa
    10441 acagtaaaac atattgccat aattataccg ccacccacca ttttccttaa tcgcgattgg
    10501 gttggggtaa tcgacttttt gaccacctga tttttccttt gaaaaagatt cagctaaagc
    10561 aaaactatgc ttcttcccgt ttttcccgac atgatctaga tacccgccac catagttata
    10621 ggcctgaata atactatctt gatcacaccc tagttctttg gaacgcttaa ccaaagacgc
    10681 aaaatagaga cagccttgtt tgatggatgc ctcggtactt aatgaattag gtggtaatcc
    10741 ggctgactca gaactttgca taacgtcttc cattgtgcca ccagattcca cttgaataat
    10801 cgccaaaaga attggaatat actctgttat atccgattct ttggcatact tttccaccat
    10861 tgcacggtgt ttcaatactt cttctgagac actagttccg ccaggagaag tgccagaact
    10921 acgatttccc tcactgtctt caccaaacaa caaacccaca cacatcaaaa tacccataaa
    10981 agagatggtc agaaaagtta atgaaataac acccactttc ttcaagttca ttttttctca
    11041 cctgaccttg attttggacg atggttcatt cttttttgta ctttttgatt ggtctgcttt
    11101 ggcagagtgg acctctgttt cacttgttgg aaagaatttt tcttacttgc tgattggctt
    11161 ttcccttttg aaatgatttc ttgactagtt agttttcgtt tgatttcggg attcagattt
    11221 gctctcacaa tccgctttgg tgcttctttt ttcagcatca tatctttctg aggttgagct
    11281 gctttatcta ttggaatatt cgcccttctt tttccctgac gatttgttgc ttcactcagt
    11341 atcttgcgtt tttcgtccat tttcgcattt cttcgttgtc gttgtttttc tcgcaaatcc
    11401 actcgatccg ctttttcttg gaacagtccg cgcttgaatt cttctggtgc ttttttcgac
    11461 ttttcgattc ctttatggag attgtatttc aaattcgttg gcgtattcct tacctgatcc
    11521 tttacttgtt tagtcttatc tttcaccaaa gcttgggtat ccaaaacttt gccgattttt
    11581 ctcccagcca aattcattcg ggattgtttc ttattatttg aaactggatt ctccttagtt
    11641 aagtcagata gtatttcgtg atcgttcggt aatcgtgaat tctttcgtag agaagaaccg
    11701 gatggcttaa atttggattt tgattgttcc tttgccaact tatttccgac tgctgcacca
    11761 ccaccaaaag ccaaagctcg tcccacattt cgattgagtt gccgcaattt tcgattcatt
    11821 aacatttgcg gttttcgcat gacgcgtctt cccattgact ggctatcatt actctggagg
    11881 ttgaacatac tcataatgtc gcccaatttg aaatagatac cggcaaaggt cacaatttgc
    11941 agaaaagcaa ccatgaaaaa tggataggtt gccgaaatat tgaaaaacat cgtggagatg
    12001 ctaaatgcag tcgtaatcac caaagtaaca cccgctcgca tcataatcgt attgaataaa
    12061 cgaataatcg ccttctttcc taaactttca taagtcggca acatggacaa taagaaacta
    12121 attggcagaa acatcgcaaa gataatgaag agaatttgcg agaacaacat aatccctgtt
    12181 agaagaaaaa caaagaagga aatgccaata ttgaacaata ccaaaaagac cacggtccct
    12241 aaacggttca tggtcttggt aatactgaga ttcgcattgt cattgtcttc aatctctgcc
    12301 ttgaccgctt cttcccgatc tttgcctttg ttttcatctg gactcacaga tagaatcttg
    12361 ttgacccggt cttcaccaat ttcttctatg ttggaatcat caaattgcag tagtaaccaa
    12421 ggttgttgga cttgaatcga aaacaaacta tcccgaatca aatccacact atcttttcct
    12481 tggctttctg aattgggtac aacgatctcg gttccaaggg taagggccgc ttcactaaca
    12541 tctgagctaa agtcattgat ttttgtgata taggtaggcg cataagcaat gaacgaaccc
    12601 gatagtagaa aaatcatcac aaaattaatc acggctcgaa ctgctttcgt ggtttctcgt
    12661 tttaataatc cggtatacgc cacatacacc cccagagcta aaatcatcaa taatagaaac
    12721 ccaaaataaa atccggaggt ctggagtcca ttttcagtaa taccagccaa tgtttggata
    12781 ttctttccaa tactttcagc cgtatccgaa atgaaatcta atttgtaagc ctcttgaacc
    12841 acataacccg tggcgttcga caaatacaaa ctcaccgtcc aaatgaaatt agtaatggca
    12901 taaagaccat acatcacgct tttccctaag ccatcacccc aattccaagg caaccaatcc
    12961 caagagctat ccacaaagaa atctagttga tagttgttca gtgaatattt ggaatataga
    13021 tttcctgctt caactgtatc atcaactagc cctgtcgcct cggctaccgt accaactaaa
    13081 ctcaatagaa ctatcagagt gagtatgcta ataaaaacga ttcccacaat ccgaaaaagt
    13141 ttctttttca tcccctcact ccattctctc ttgaacaggt ggacgggtat caaaggcatg
    13201 gaagagttct tcaaagactg gatgtacctg aaccacgcct acccgaccat acaaatcctg
    13261 catcaagcat tggccatttt ccaaatctcg taggcgtttt tgattgcttt catcttctcg
    13321 atccaagcca aagaattcca aggttttctt gatttcatga atatcggtac tgcgaaacgc
    13381 aaacttcaac ccgatattat tcttcaagcg ttcatccagt aaatcatcac tattttgcgt
    13441 gacaaagtaa accccggcat tcatagcccg cccagcacga acgagcttat tcgataatgt
    13501 tttcccttgc gctacttgaa gaaaactcca cgcctcatct aagtctacaa ttttgaagat
    13561 ttctcgatta ctatggataa agtccaaggc aaaagtggaa atgacgatta gcatggaaac
    13621 actaagcaat tccatggtgg tatattcttc aaaagtggtt tctgcatctg gtaaaaccaa
    13681 atccgctact tgaatgatat ttaactgcct atctaaacta atcgactggg taatcgaacc
    13741 atctgagaaa agcaaatggg caaaatcata atcggtaaag gactcgatgt gatccgcgat
    13801 attttccgca actggtgagc cttcttttcg aagctcatta attacttgta ataatccccg
    13861 ttgattgctt tgggtaaccg tccgaatggc cttacgcaac actggaaact tctccccatc
    13921 tcgactagaa attcctgtta aaaaggtcag aatatcaatt gctagacttt cagaatcctt
    13981 ctttttctcc ataatcacat aaggatcaag taatccttga ttttttggct tactagttaa
    14041 gttgagaata ttgatttcat ccgcaatttc tggcaaggtt tctttccagc caccccgttc
    14101 cccttttgga tcgacaatca cagcttgtcc accaaaaagg acggcgtaat aaactaagag
    14161 attgttacta aaggactttc cgccaccgag tgaaccgagg aatgccgctg ctagcgcatt
    14221 ggtaactgaa cccttgaccc cttgtgaggc caatgccggt ttcaggtaaa cattgcgccc
    14281 cgtatctacg ttataaccaa agtaaatgcc ttccaactcc cctaacatct gagtggcgcc
    14341 aaaaccaagt ccggcgagaa aatcagacgt aacatactgg atataatcat tcatgtatcg
    14401 cttactagaa ggtaagaatt cttcatgtag ccccatcata tcgccaaaag gccggactag
    14461 ttttacattg gtatcatcat aaaaatctag cacttcatca caccggcgct ttagttcatc
    14521 tactgattcg gcacttactc gaaccacata gctcagtttg tacatggatt ctttggactg
    14581 atccaaggta gtttccaatt catctacgga atccaatgca tctaagacat tggaatttgt
    14641 ttcattgtca gattgatagg catggttgtc caagtctttt aattcctttt tcttattgcg
    14701 aacggtagca agtgcctttt tgttcgtcac aatctccaca ttcatactag tgtcaattgg
    14761 gaatgtaaat tgttgttgct ggtaatagaa aagttctgag gagggaaact ccatttcccc
    14821 cacaattgta ttaattgtta agtacgctac atatgattca tgattctcat gttccatgca
    14881 taagtaccgg ggcttttctt caatcaaaca acgactggga cgtaataagt cgtaacgttt
    14941 caccaaagtt tccgacttca gctttttctt tggtagctga aattcatatt cttcaaatgg
    15001 tgtacccttt tcgccataga tatgttcaat caaatacatc agatcactcg gtgtgactcg
    15061 tctgacttta aatcttcgag ccaatttact ttccattagt ttttcaagct tggtatatcg
    15121 gcgaatttct tcattggata aggaaacaaa atcgcccatc aaatgatgat tcactccata
    15181 gacaaattct tgaaagccgg agaagaaaga tttcttgaga ttctttagat tgacctcttc
    15241 atctgtcgca atcagtttga agccaataaa aaagcgataa tcaatctgac tatcgccaat
    15301 catggatact aatgcctctg tctgtaaatc aattcgttgt ttcgctactt ctgttaatcg
    15361 cccggtaatt tccttttttg atcgttcttg cgtggcacga atactacttt ctgtggcaat
    15421 ttgtaaggca tgaatcttgc cttcacgatt ttgcgcaatc aattgacgaa agttatcgtg
    15481 gacttgatat ttctgttcag gtgacaaaaa ggagtaattg taaggtatta actcatagta
    15541 ggcaaaacat tctccctctt gattaaagac aagattattt tcaatatact taatcgggta
    15601 catagttcac actccttacc agagtaatgg gttcgtccca tcgtttcttc tgttctttga
    15661 ctttctttcc tgcgaaagtg accttaaatc gtaagtgata gctaataaaa gatcgtaaaa
    15721 atcccaatgg tttcttaccg tcaaacgttt tttgcgacat gaaccaagta actgccaccg
    15781 gtatacctaa gtattttaaa aaggccccct ctatcataga tagtggaggc acatgggcaa
    15841 atacgatgac aaacagcaag gataaaacaa accaagccat ctggttaaaa gtcacaggaa
    15901 aaggcagttg aaagtcatta attgcataaa tgaccttttc aactgcccaa atactggtgt
    15961 agctttttat tttcttcatc aaatcattcc tttaaaaaga aggatagttt agaaatcaac
    16021 tacccttcaa taattttgtt aatatggata ctcaaaaaca cctcgactcg ttaccaaata
    16081 attgcctcca atctccaagt ctctaccaca cgcatcaaaa tcaatatacg tcttcaagtg
    16141 ttctggtact tctccaaaat caccagcttc tacaatcaag aaatatgcta catcactcat
    16201 atcatcgcaa tctggataat agataatatc atccacatgc tctaccatct cctcaaaaga
    16261 attgaagaaa gcatgttgaa tctcagaagc aacttcacca actggcgttc cttcaagttc
    16321 ctccgctaag ccacacaacc gattaatttc ctcaattggc gtatactcat caatctcaaa
    16381 gggaagttca taatcatgaa ttgcatattc ctcgtattcg tcatttaacc caattcgttc
    16441 tttcacttca tcaaaatcga caggtggggt aaaccacgcc cccaccaatt caccttcgtt
    16501 atatttcccg aggttcgcaa tataaacacg catctgttcc atcgtgcttc ccccttacgc
    16561 accgacaatt cggttaaaca aactgagtaa gacatccttc accccagaag cattaaagac
    16621 caatccgacc gcaatcaaag caatcaccaa aaagccaatc agtttggaaa actcccgctt
    16681 aaatcctaaa aacaaaccaa tgaccacaat tgccagtaag actaaggatt gggcattggt
    16741 taaaaaccat tgatataaat tttgtccgaa attcatcttg tcactttcct ttcttttcca
    16801 ttgctcgatc cgctgctgca cttgcattca tcaagcacat cacagtaaca ccaagcatac
    16861 tgccaccaaa taccaaaaaa atatctttta caattcccat gacgattcct cttttcatat
    16921 aatcaaattt tcaattgcca tactttgttg ctccaaaatc tttttatgtc ggtctgataa
    16981 gcgtgcattc tgtaccatat cctggatata ggttgtccga ttcaatcgat ccaactcctt
    17041 agccattttc cacgtagggg cgacttgtcg gcctagccaa ttcaaggttc gttcaatggt
    17101 atacggtttt ggctcggaag ttaattgaat ctctttccga ttccgtccaa taaaacgccc
    17161 ccattgttca ttcaagggcc agttggttcg ccttttacct tctactttat ccgcaaatcg
    17221 caagtaacga ttgataatgg aaaacgtggt gctctcaatg ctacgacctt ttaacaaatc
    17281 ttgaatcgca tgaaaagcac ggtcattttt cagtcgaatc tcaaaacgat ttttcgtctc
    17341 agtttggtct aatggaatcc ctaacttgat atattgttca tagtcttttt catacaaaca
    17401 gaaataaacc tcactcttga gactgccgat atataaggta ttgcccatgc cgtccttttc
    17461 atcagccttt aaaagttccc cggaacgata acttttaaag gtacgaaaca aggagataca
    17521 ttcttccttc tgacattttt tcgttagctc aggtatatcc aatagcccca ctcgatcatt
    17581 gattgccaag tccaagcgtt tcatcacgcc accggccttt agacaatcct cgaaaaagtc
    17641 gtaccagcta cgcttttgag cgagtaaaaa agtttcaaat tgtcggcaac ctcgcccctt
    17701 taattctaaa agcacacctt tatcttcttc atgagacaac atcaccacaa tatcacccat
    17761 gacatactgt tcttgatagg agtaaaaggc ataatcttca tggagcatat actcaaattt
    17821 caaatggaga atttcttgaa taatttttcg tgcatccgtt gtgggaaagc gaatccttac
    17881 atagtcaaac agtaacgtca aaggcaaatc gggattaaag ctttccagtt ggtcaaaaat
    17941 tttcctcatg gtttttatag atgcggttac gccaccattt tctatctgat ttaagtacgg
    18001 ccgactaagt ccacaagccg tagccaattt attttgtgaa acgccgtatt cgttgcgtcg
    18061 gtcttttaat agccgataat ctaaattccg ttgtgccaag ttctcacctc caaattcaag
    18121 gtgttaactt tttcaaatga gttaacacct atacaaatta cttactatct ttgatacaac
    18181 aagcattctt aatggttgct tacttatttt ttgtgttttt gtacccccct gttagatacg
    18241 ggggtgagac atcatggtcg gctaacgccg tccatgaaca ggcacagcac cacggaggat
    18301 ttctgcttcg cgaatcctcc gcggtcagcg cctaacagtc agttgtgttg gtttaggtta
    18361 ctcatctgta ataccattta cttccaactc tgctttcatt tctaacaagt attgacgaat
    18421 atctggttct tttgcttggt ccgcaatccg ctcaaattct gcaattactt gatttatttc
    18481 aattcgagca tccgtctcct cattcattaa atatggatcg ccagcgactt tgacaccatt
    18541 cttgcgaata tcaaatttta accgtcgcaa ttgttcttct aattctgggt ctgactgaat
    18601 ctttccaatc acccaataaa tttctccaat caactcatca taaaccatgt acatccaact
    18661 cttttaatct tctggcaata tcaaaaatga ccggaactgt tacagagttt ccggcttgct
    18721 tatacaactg actatctgaa ttgacttgag aatctcgatc aaaagcccaa tcgggaaagc
    18781 cctgaagtcg ccaacactct ctaggtgtta gcttccgaat ttgatactga tccgtgacga
    18841 tcgcttgttg gtctcctgtt agtaaggtat gaacactctg ttttcccaga cgacctcggc
    18901 gacttgtgga attaggaaaa gccagattca cactgtcttc tggcttcacc tcttgatatt
    18961 ctgtttcatt ttttcgaatc atgatttttg taccaccttg tttcgttgac aaggtggggg
    19021 cggttccagt aacttcataa actcgattac cttgttcaaa ctttcccggt aatagtcctg
    19081 ctacagagat tccatccctt gctggcagtg cgattttgat tccttcatca cctgttaagg
    19141 ttggcgctac gccatctgaa ccatagactt gtccacccat tcctcgattg gaaggattga
    19201 tatttaccaa atttctgatg ttttgatcag atgttgaaga cggtccaatg ataggaaata
    19261 cttgttcggt acatgcgtct cgagaatgtc cgataaggaa tatccgctcc cggctttggg
    19321 gaatgtaggc ctttgagtta agcacctacc actccacgtc ataccccaat ccatccagcg
    19381 ttcggaggat cgtctcgaac gtagcccctc cttcgtgatt gagcaatcct cggacgttct
    19441 caaggaataa aagcttaggt cggagaagag tggtgaaccg agcgatttca aagaataaag
    19501 tacctcgagt atcggaaaat ctttgccgct ttcccgcaat tgaaaaagct tggcacggaa
    19561 atccgccgca aaggatgtcc actggtccga gggattgaat aaactcatct gatacacttg
    19621 tgatgtcatg catttccacc tccttacttg tatcatgaat tgctttataa ctacgtctag
    19681 caaatttatc aatttcacaa aaaccgatac aatgatggct cgcctgttcc atacctaaac
    19741 gaaagccacc aataccggcg aataaatcta aaaatctcat acattacctc attcattgca
    19801 aagaaaaaag ccaagaacat tttgctcttg gctcatacat tattaatttg tcctactatt
    19861 ttttatttaa tccaactaat ttccgttgtt tttcattggc ctgatcggct acatatttaa
    19921 tcaccgtttt gaactctttt gcagtagcat tttccgataa atccgcaatg ctcttattgt
    19981 atttctttac aattttcgtt acttgttctt tctcagtcat gtaattgaac ctccaaatct
    20041 ttcctactat accctagtgg taaaaataat tctagattat ccgtagcata atgaacttcg
    20101 gaaatattat tcgtataatc ttttattatg atatgaagca atacatctgg atgtttactc
    20161 tttacttttg caatgttctc tcttgatttg aaaaaagcgt taataaatgc ctttttcggc
    20221 acatgacgtc cctctgcaat ttcacgttct tgtgtaaatt tccaagcgat tttagggtct
    20281 tgataaacat agaaaatcgt cactcgaaaa tgttttttca acgcccgcga gatatttttt
    20341 tcagcactta atattgcaaa agttgcatcc agaataaaag aatagccatg atctaagaca
    20401 tatctaaatg cctgctctac caaccaagaa gatgcttttt gaaagagact agagtttttt
    20461 ccattataat caggaaactg ggtgcgaaag taatccgcat caataatcac atggttatca
    20521 taattctcag ctaatccaat ggcaacctct gattttccag ccccaggact ccctgccata
    20581 aaaactgcgt ctcgtccaac ttcgggggat ttccctttga taatataaga aataaactta
    20641 tccttatttg cttttgcata ctctaaataa gaatcattac tttccatatt aattatcccc
    20701 tttcttccta ctatataaaa gtattatacg ttacttgaag tataaagtca gtattgaaga
    20761 tcactcaatc attcatcttt tctaatttac taattgaatc taagaaatca tacccttttg
    20821 gtacaagtgg cgtataaaac tcactaatca cactgccacc tgtatccaca taacctcgac
    20881 ctttgatgtg cttcataaag aagttttttt cgacctcccc aaacatcatg gaatatccga
    20941 gttcactcat acgacccaaa gccacgcgaa agttgaattg atcgcggatt ccgtctccta
    21001 aatatttcgc atccggcctt tggcaagcca aaatcagaaa gaaaccagat tgacgaccca
    21061 acatcacgat ttgtttgagc ttattcaaaa tcaccgcact ttctttcgtc gttaacatct
    21121 ccatataagc cacgtattca tcaaagatta aaaagttggg tggaagtcca agataggcat
    21181 aattctctcc tgttttgtag ttagacattt ctttcattgc cttgctacga gccatcatac
    21241 gttcatagaa atcttccaca caagcagaaa tttcttcctt ttgtgaataa acatgaggca
    21301 tcaccgtacc taaatcagct aaatctgcat ttttgggatc gaggataaac aattctgcat
    21361 ctgacttcaa tagtgcttcg ataatggtga ggaggaaata ggtcttccct ccacccgtgc
    21421 caccagcgat taacatatgg ggtaaagaat cataagccca tacttgattt ttcatcaatc
    21481 gcagagtccc attttctgcc actacttcgt ctatcccaat tcgattggcg atcatatcat
    21541 acagtaaagt atactccaca taggagtctt tgagttcctt ttctactaac tcacaataca
    21601 gtccgctttc taacttcttt tccaatttta aaagctgatc ttggtatttc ccaagtgaaa
    21661 tttgaacgcg aatagacagt aatccatctt tcattcgata atagattttg gggaagtaac
    21721 tgatagtctc tctggttcgg ctactattca aatctttgaa aaagccttca ctttgagttt
    21781 gtttcacttc ataccaatgg ttctctagaa tcatccgagc tagcttttgt cgatgccaca
    21841 agcttcgcca atgatcaaga aaaaagtgaa tataaagcaa tatcatagct cctgaaaaag
    21901 cgagacttcc aagcagacat actaaatctt ttatagacca aacgatttca atagtagaaa
    21961 gtgatagctt agaaatatca aattgtctaa actcttgaaa ataaaagacg ctcatccaac
    22021 cgatgaacag agcgagtagc cagccaagaa caaagtgata aaccaagtgc tgatctcctg
    22081 cgcgtatgcg atgtcctttg tacattttga tggtttccac ctccaatcga aaaagcccaa
    22141 taggatttca ttaggcaatt tcaaaaagtt atgctagtca tttactgcta cgaggagggg
    22201 agttgtttcg tttcaaccaa ttgatttaaa aagccttcaa tagcatccgg catctccaca
    22261 ccaatttctt ccaaaatgga ttctgcctgc cgacataagt tttcatttac ttcgacaact
    22321 ataaagaaag actcttcttc tcctaaccag tctagtacat cagagtcttc ttcactgtcg
    22381 ctattttctc caagtaaacc ttgatgtata atcagaaaca ttttttcatc agaggttaat
    22441 gctttaacga gttgttcctc agtcagctcg ccttttccat attgatcgag acaatctaaa
    22501 atatgttcaa cattaatttc cataatcttc acctatttct ttctttcatt ttggttcgta
    22561 ggatttcctt gattcggctg ccgcttcaag actaaatcat ccgccttaat gaaccaatta
    22621 acggtagttc cttctcgaaa aacataattg gcaacggtat ccacgattgg attgaccaac
    22681 tctacctctg catcataagc aaactcgcgt aagggtacat tagctgggat actaacttga
    22741 atcattcgcc cttgtgcaga ggattttaaa ttataggttc gttcttttac ctcattacca
    22801 attgttccat cctcattttg cttccgtact tctcggcgta atgcggaaaa acgcaacttc
    22861 ccaaatgtta aacctgaatc tacgacaata ccttctgtta atctcatctt cttttcctcc
    22921 tatgctttca ctaaatcatc aacatacaac acgtaatcag taaacccacg attctcaatc
    22981 tcatagcctt tggcaaccaa atggggattc acggctttta gcggttggtc ttgatcaaaa
    23041 tccttttcac cggcttccgc agctactacc acctcaatat catcggcccg ttgcttactg
    23101 gaatacaaat gataactgcg accaatcacc gtagtattcc gtccatatcc ttctgttata
    23161 atttctcctt caccggcata acttaacttt ccaaaggttt cagccatatc tggcacgaca
    23221 aatttcaatt ccatcttctt ttcctctttt cattgttagt tttggataca aaaatagcgc
    23281 gactactttt ttaggaggcg gtcgcgcacg ttggttctta ggatttactt gtatcatctc
    23341 atcaccttag ttccggcgat tccagaaata atagcccgcc gtcgcaaaaa tcaaagtaaa
    23401 accaatgaac agtaaaacat tggaattttt ctccccggtt tgagggaagg tcttcactgt
    23461 gggactagat tcttgagagc cacttggaac agttggtgtt tccggttgtt tcggcgtact
    23521 tagtatggtt ggaacttctt ttggtgttaa ggtttgagat ttttctttca aatcttcatt
    23581 gtgttttcca ttgatattcc catctttatc gtaattgatt tccgcaaaag taaacttggt
    23641 tccttctgga tattttccgg tatctacttt tttcgcaagc acagttttgg tgaattcttt
    23701 atcatttact tcataatcaa tcttgccaga tttccaaatt tctttgtttg taccatccgg
    23761 taataaagca tacagaattg tttcgaaagc ttcttttgag ccatccagta catcatgagt
    23821 aatcgacaca tcatcaaaca tatccaccac atcaccatga gtaaaggtct gagaaccgtt
    23881 ttctaggtgg gcctttgttt ggatagaaac atggtgttcc actgtacaac tgacagtttg
    23941 ggcttggtta ttcaagctaa catccttcgc tactggctta tcgcctgctt caaaagcttc
    24001 cttattttca tacacatagt tgaataagac aatagtttta tttaaaactt gttcggcggt
    24061 taatttatgc tgaattttcc aagttcctgt tttctctttg ttcgccaacg ttgcgcttgt
    24121 ttctgcaatc accactggtt tggctttttc atctttttct tgggcagctt ttgtcgcatc
    24181 cacatcaatg gcttgcgcta ccacatacca gtcttctttg atttcatgta gattgtaact
    24241 caatttatca actagctcgg tagaatccag agaagttaat tctttgtttc cgtctttcca
    24301 agtcgcaaga gaagttaaac tatcttcttc ctcaggcaaa tcatagagca ttaaacggtt
    24361 caaactaaca gaaaatgctt tgttcgtcag cttctcgtaa ggaacggtca ccgttttaat
    24421 tggttgtttt tggtcttgtt cggtaatggt aaagacatac tcactcttca caaagtcttc
    24481 tttgttttcc ttaaaagtag aacggatttc taatggcgta atcttttgaa aaccttctgg
    24541 ggcttctact tcttcaagta aataatcccc ataaggaaga ttttcaaact tgccataacc
    24601 atcaaaccct aattgctcgt tataagctgt ggtcgcttca tcttcggcac ctgtgatttc
    24661 attggtccct tccaatggcg acactttaaa ggtcagatca ttgaagccag tttcggtagt
    24721 gcccgcagct gatccagcaa atttaaagaa atcaaagcca aagcgaatca cttgttcttt
    24781 tgccgtaaca tttcgggtaa tcacggcatt tttttcgcta tcatccacct ttttgatgga
    24841 tacaggatac tttgtttcat caaggctata tccttcgggc gcttttgttt cttgccaata
    24901 atactcgttg atagctaaat gtttaacggc aacttggttc ttttcgtcta aagtcaaagt
    24961 gaccgtttca tcagaacctt tcgttccctt cgctaactct ggtttaaaag cctcactcca
    25021 tttaacagct tttccatcct tagcagtaaa caaggtgtat tcagcaccct caaagactgc
    25081 cttaccttgt gctttatccc cagtatcttt gtcttctttc gtcaaagtgg tttcgccagt
    25141 gacttcttga ttttgccctt ttacgttact ggtaacaagg gctacggttt gattggcata
    25201 cttcaattcg acttttacag gtttgaacgt attcacgaac ccatgactag ctttagtttc
    25261 ggtcacataa taggttccca attccaacgc attagcaatc tcttttggtg tttccgcatg
    25321 accgttttca tccgtggtca tttcttgaac gatttcaccg gttggactgt ctttgcgaat
    25381 ggcaaacgta ttaccagcta aggaataatt gtcattccaa agatcactcc ctgtttcaac
    25441 tccgctttta tccagaatga tttgcccttt ttcccgtgcg ttttttgaag tcacgtaaat
    25501 ggtttcgcct gctttaatgg tggtagtcat gggggtggta tcaatcgtat aaggcgccgg
    25561 tactgatttt tcggtaatgg ttacttttgt accatgagga atctcatcca atgtagcaat
    25621 gccttctttg tcagtagtca cgtcttttgc aggtaaagtt tttccaaagt ctaaatggaa
    25681 aaccgttcct ggtacaacat cacccgattc tttatcgact tttttgatct ttaaagaacc
    25741 ctccgtttcc acatcaattt tgattgtgta ggtattaggt ttatcaatcg ccccggccat
    25801 taaagtctgt tggccagcct ttttataggc gacaggtgtt ccggtaccag cagatttctt
    25861 aagcgtcagc acaccacttt cttttgaatt ggcatttggg gtgattatca actgattacc
    25921 attcacacga taatcaatgt ttgcggcatt ttccacaacc tcatcgaact ctgacaaatt
    25981 cagattattc gtatccgtca cagtagtcga ttgccctaat actgttttag ccgtactatt
    26041 atggaaggtt ggtttcttct gataatcgtc aatggcttga tttatttttg cctcaatggc
    26101 tgcgatattt acggcactac catctgatcg ttttattgag tgaagggtat acccattaac
    26161 ttcttgccag atcatcttct gtgccacaat atgagtatct gcatctgtac ctactttttt
    26221 ccataaaacg gaaactaatt tggctttatc ggacatatct ggtaacgggt ttttctcata
    26281 gccaggagtg aactcattgt aaatcgggac tcctggttca atacagaaaa tatcttgttt
    26341 aaagccgttg tacactgcat aatgcggaac agtgacttct gacattggac gaccatcact
    26401 aaaagttcct tcaacagcat attgatgtgc taaatctagt tctacggtta ccgtttgtgg
    26461 atgggtaatt tcatcagcaa aagtaatcgt aggagcaatt gctccaacaa ttgcttgaac
    26521 agccaaactc aaaatagtta atacacataa tttctttttt tgttttttat acatgaaatg
    26581 ttctcctttc aaatt
//
LOCUS       DAIGOK010000027        25967 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.027, whole genome shotgun sequence.
ACCESSION   DAIGOK010000027 DAIGOK010000000
VERSION     DAIGOK010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25967
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.027"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            50..952
                     /locus_tag="NOA01_000904"
     CDS             50..952
                     /locus_tag="NOA01_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCI0632116.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(993..2879)
                     /locus_tag="NOA01_000905"
     CDS             complement(993..2879)
                     /locus_tag="NOA01_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCI0632117.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLKAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(2976..3827)
                     /locus_tag="NOA01_000906"
     CDS             complement(2976..3827)
                     /locus_tag="NOA01_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCI0632118.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQNNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            complement(4150..5934)
                     /locus_tag="NOA01_000907"
     CDS             complement(4150..5934)
                     /locus_tag="NOA01_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0632119.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(5934..7685)
                     /locus_tag="NOA01_000908"
     CDS             complement(5934..7685)
                     /locus_tag="NOA01_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCI0632120.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(7827..8051)
                     /locus_tag="NOA01_000909"
     CDS             complement(7827..8051)
                     /locus_tag="NOA01_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HCI0632121.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            8302..9888
                     /locus_tag="NOA01_000910"
     CDS             8302..9888
                     /locus_tag="NOA01_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCI0632122.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
     gene            complement(10197..11972)
                     /locus_tag="NOA01_000911"
     CDS             complement(10197..11972)
                     /locus_tag="NOA01_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HCI0632123.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     ILASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPTVEIIIA
                     CLLAIYYRKYNSSKKLLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYGIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFDFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVIGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIAVTSMLVFSKLNLKKALSSK"
     gene            complement(12183..13085)
                     /locus_tag="NOA01_000912"
     CDS             complement(12183..13085)
                     /locus_tag="NOA01_000912"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCI0632124.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            13364..15709
                     /locus_tag="NOA01_000913"
     CDS             13364..15709
                     /locus_tag="NOA01_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCI0632125.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKDEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            complement(15872..16138)
                     /locus_tag="NOA01_000914"
     CDS             complement(15872..16138)
                     /locus_tag="NOA01_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632126.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQTWAEKRLTDQNHQ
                     MTAEEATQEFSNRHAHLKESVKGQFAEKLTELFQRQEKKLKQFR"
     gene            complement(16131..16454)
                     /locus_tag="NOA01_000915"
     CDS             complement(16131..16454)
                     /locus_tag="NOA01_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632127.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            complement(16466..17722)
                     /locus_tag="NOA01_000916"
     CDS             complement(16466..17722)
                     /locus_tag="NOA01_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HCI0632128.1"
                     /translation="MTITDKQYKGISNKVYWLDPKHPQYSKSYKEGAIKNFGETKFQI
                     LKIKNSFDGMQAMAVAPIKNGKVDISQVVIAFAGTNLSDWKDLETDARSVMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAYLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSVKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            complement(17719..18114)
                     /locus_tag="NOA01_000917"
     CDS             complement(17719..18114)
                     /locus_tag="NOA01_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632129.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREAQKQQDLLAVEKQS
                     VKVLKNTFADIKEVKIEQFEKNDMTGIYEALITMTNTEGKAVYFDYSFTKESNEIDGY
                     GVENEEVQKEGITVDEIIVIYTDGSEEKV"
     gene            complement(18468..19469)
                     /gene="ccpA"
                     /locus_tag="NOA01_000918"
     CDS             complement(18468..19469)
                     /gene="ccpA"
                     /locus_tag="NOA01_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HCI0632130.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            19679..20782
                     /locus_tag="NOA01_000919"
     CDS             19679..20782
                     /locus_tag="NOA01_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCI0632131.1"
                     /translation="MNNVKINELRQWMAQHQLELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            complement(20845..21459)
                     /locus_tag="NOA01_000920"
     CDS             complement(20845..21459)
                     /locus_tag="NOA01_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCI0632132.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQDLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(21452..21901)
                     /locus_tag="NOA01_000921"
     CDS             complement(21452..21901)
                     /locus_tag="NOA01_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HCI0632133.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFLKTKNILKRQEENKNG"
     gene            complement(22026..22964)
                     /gene="galU"
                     /locus_tag="NOA01_000922"
     CDS             complement(22026..22964)
                     /gene="galU"
                     /locus_tag="NOA01_000922"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCI0632134.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            complement(22979..24004)
                     /locus_tag="NOA01_000923"
     CDS             complement(22979..24004)
                     /locus_tag="NOA01_000923"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCI0632135.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHHLPDSIRAFTDMEDAVKDADAVLFVIPTKAIRSVAKELVAKLNTKPVI
                     IHASKGLEQGTHKRISEILLEEIPTEKRKAVAVLSGPSHAEEVAKHDITTITAASTNV
                     KAAKYVQELFMNHYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            complement(24048..24878)
                     /locus_tag="NOA01_000924"
     CDS             complement(24048..24878)
                     /locus_tag="NOA01_000924"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCI0632136.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLVLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGSLPDYDRSLGKNQQII
                     "
     gene            complement(24893..25831)
                     /locus_tag="NOA01_000925"
     CDS             complement(24893..25831)
                     /locus_tag="NOA01_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HCI0632137.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
BASE COUNT         7445 a         5456 c         4684 g         8382 t
ORIGIN      
        1 tttcaaaaaa gttataattt acgtaaaaaa actagaagaa ggtatctaca tgttattacg
       61 tgaaaaaatc aaagaaatct ccttttctcc ttcagaaaat gaagtgattc agtttctctt
      121 aagcgattct ccagcacttg gcgaaatgac catccaagag attgctgagc gatgttatgt
      181 ccacccgtcc acactcatcc gtgtagcaaa aaaaatgggt tttgatggct gggtcagttt
      241 tcgtaatgct tttttagctg aaacagccta tcttgaaggg aattttcaag acgtagatgc
      301 gaatcttcct ttttccacca cagacggtat tatgacgatc gcaaataaaa tggcttcttt
      361 agaacagatg acgattcaag atacactttc tctcattcat catgaccaac tgcaaaaagc
      421 caaacaacta atgcttcaag caaaaaaaat cgtgattttc gccagtaact cgaatacttt
      481 gatttctcaa gatttcatgc tgaaaatgaa gcgcatcaaa atggatgtca cgattgtcac
      541 cacttttggt gaatcgttct atgaagcgta caattgcacg agtgatatgt gcgggattct
      601 gatttcttat acgggtgaaa acaatgtgat ccttcgtacc gcagaaatat tgaaacaaaa
      661 tcatgttcca attttagcgt tgaccagtat cggtgattca acgctatcct caatgagtac
      721 agccgctttg cgacttacga cacgcgaacg actgtattca aaaattggta attttacgat
      781 caatacatcg atttgttatc ttctagacgt gttatatagt tgtgtttttg cagaaaatta
      841 tcaacagaat ttagtccatc taatttctgt aggacaaaaa tttgataaac gccctatcag
      901 ctcagccatc atgaaagagc ctttcaattt tgaaaataaa gaagtggact aaaaagaata
      961 gttaagtcta ttccattctt tcacctaacc gttcaggtca gtgcggtgat aagtgcatcg
     1021 tctggcccga ccgtctcccc ttgattttcc aaaatatcca aataatccat gtaattggtg
     1081 acgattacag gtgtttctaa actgtatcct tccttttcga ttttcttttt gtcgaagcgg
     1141 agcaacagat cgccttgttt cactctttct ccttgctgca ccaagctttc aaaatatttc
     1201 ccttccaact gaacagtatc caatccgaca tgaatcagca attccagacc tgtttcagaa
     1261 accattccga ttgcatgttt cgtggggaac agggtcatga ctgttccgtc aaaaggggca
     1321 cgtacttctc ctttttcagg gtagatcaag attcctcgac ctagtgttcc ttgtgcaaaa
     1381 gctgcatcct ttgctgcttt cagtggagcg atttgtcctt gtatcggtgt ttttacaatt
     1441 tctttacctg atcttttctg tctgtcatct gtttcacctg cttcctcctt ccaaaagaaa
     1501 aaagtcagcg caaaggatac caccatcgca atggcgatcc caatcaatac attaatcaca
     1561 tctgttaaat caccatctgg tctaatatag ttgggaattg taaagatgcc tagtccacct
     1621 tgattccaag aagttaaatt cattaaccca ctatacgcgc cccctaacgc accaccgaca
     1681 caagtaaaaa taaatatttt tttacgtggt aatgtaaacc cataaatagc tggctctgta
     1741 atccccacaa taccagaaac aatggctggt attgcaattg ctttgtcttt tggatttcta
     1801 agcttaaagt agagtgcggt aagtgctgca gtttgtccaa aagacactac tgcaacaggt
     1861 gtcataatat tttgccaacc attcacagaa aattgcatca atcccattgg tacgattgcc
     1921 caatggagac caaacatcac cagaatttgc cagaagaagc ctacgattgc tccataaata
     1981 actggcgaaa gtgctaaaag tgtttcaaat ccagccatta aaatatcagt tgcgaatgtg
     2041 aatactggtc caacgagtaa aaaacctaaa ggcattgtaa taagaacggt gaagaatgga
     2101 acgaagaaat tcgcgatcat ctcaggtact aactttttaa ttggtttctg aatttggcta
     2161 gctaacaaca caataaagat aatgggaatg acagaagaag cgtagttacg cgcaatccag
     2221 ggaatgccga atactgtttg ataaataggt gcttcaaaaa tagtgccaga aaatatggtc
     2281 gttagtggtg cttctgctgc accactcaaa gcttccagcg acaagctagg attcattaat
     2341 gctgcgccaa gcaacatccc tacgaattca tttactttaa atttacgggc agctgttaaa
     2401 ccgataataa caggcataaa ttggaataca gaatcaccga tattatcaaa cataacaaat
     2461 gtgccagaac cgcggtcaaa cgcgcctaag aagacaagga atgctgtcag tccacgaatc
     2521 atcccgccag cagctaagat agcaaggaat ggttggaaac agcccgatat tccatcaata
     2581 agcatatcaa acggtctcat tttttctgtt gaaacagcct catcaaaagt aaggtttcct
     2641 gcttttataa cctcttcaaa cacgactggc acatgattcc cgatgacaac ttgatactgc
     2701 cctcccgctc gcattaccgt tacaataccg tccatatttt tcaagatctc atcatttgct
     2761 ttttcttcgt tttttaattt gaaacgaagc cttgtgatac aatgtgtcaa accattgata
     2821 ttttccttcc caccgacatt ttctacgatt tttctcgcta attcttggta ttttcccatt
     2881 tctattccct ccaatatatt agtggaaggt ttagccaact gaatgtcact acccttattt
     2941 catacgttct tgttaattta tcctcctttt tttatttatt tacaacgatt ctcatgcact
     3001 aaacgcgcag tatggatcgt taaatacatt ttttcgtccg tggacatctg gtagttgaac
     3061 gtctcggcta aaaagtctgc tattttctca acgcaaccaa acgtatcctt atattttttt
     3121 tgaataacct gaaacaattc ctcagaaact tctcccactt ctgatttttc tccatctagg
     3181 acgcgtgtca gaaaaaaacg taagtgtgtg acgaatcgaa aaaaatggac tgaatcttca
     3241 tcaaatgtaa tcttatagta atagcggacg atatttagaa tatcctgtat tgttatagta
     3301 aacaggtagg tattgttttg ttggctttga atgccttgtg cattgactaa atgcaatgcg
     3361 ataaatccgg cttcatcttc tgataacttg atgtggtatt tatccgagat tttcttcact
     3421 gtttttttcc cgatggcata ctctttagga aatagttgtt tgatatcata cagcaataca
     3481 tttggtacaa atattcccat tttgtgacgt tcgatagccg tatacaaatg atccgtcaat
     3541 gcaatataca cagaatcatt caactggact tgtagagcct cttttgtata ttcaatgatt
     3601 tcacaagaaa tttctaagta ctcaagagga atcgcttgtg ccaattcttg gaactgttgt
     3661 gaaagcccgt catttgtcaa ctgaaatttt ttgtcgatgc atgagtcaaa aatcgggtca
     3721 cccactttct ttttgaatgc caaacctttt cccatcacga tcatttcctg atttctttca
     3781 tccctggtta ttacgacgtt gttattcagt actttctcaa tcagcatgtt ttgctccttt
     3841 ccatgatata tagggctgta ttcaaaacaa aaagaaaaac ccaaaaagcc gtccatggaa
     3901 ccatgggaga ctcatcaggt ttagcttgca tcaggcaatc actatccaag ttgtatcgtt
     3961 ttctttatat tatcgaaagc aaacgctttc ttagtggcta ttaggcactt ttgactagct
     4021 gtcgtcgtct tttttaaatc gggtgactac gccactaacg ctcacagatg aagaatctgg
     4081 gaagctctgt tttattttca ctctagcgct taggacaaaa tgctactatg ctactgtcac
     4141 aacctctttt tacacaccac tgctttgcag tttgtacata tctgcataca aaccattttg
     4201 cgctagaagt gtttcatgat tcccacgctc tactatatga cctttgtcta aaactaaaat
     4261 caattctgca tcccgaatcg tggacagccg atgggcaatc gcaatcgttg tccgtccttg
     4321 tcgcattttt gccaatcctt cttggatcaa tccttcggtt tcagtatcga tatttgctgt
     4381 tgcttcatcc aatactagta ttttaggatc tgtcacgatt gttcgcgcaa aagtgatcag
     4441 ctgtcgttgt ccactagaat agctggcacc gtgttcgatc acttttgagt gatatccttt
     4501 tggtaattca tcgatgaact tgtttgcttg aacaaactca gctgcatggc gaatctgttc
     4561 gtctgtgata gctggattca tcatccggat atttcctgca atatctccat aaaacaagaa
     4621 ggcatcttgc aagaccaatc ccattttctt acgtaactct tccattggat attctcgaat
     4681 gtcatgatca tcaatcaaga tttggccttc ataaaattcg taaaaccgca tcaatacgtt
     4741 gatgatcgag ctttttccgc ttccggtgtg ccccactaat gcaacggtct cacccggatt
     4801 agcgacaaaa gagatattgt tcaatacatt gtgctctcca tcataagaaa agcttacatt
     4861 gcgaaactcg atcttacctg aagtaatcgc ttgatctgct ccaggatttt gttgcggtgc
     4921 atattcttct gtatccatga ttttcaaaat acgactaccg gcaaccatcc catctgtaaa
     4981 ggtgctcaaa aagtccatca tttgagacat tggattaaaa aatccctgga cataggtcgt
     5041 aaatgcatag atcagtccaa cttctacggg agaatgcaac gcatcgatcc caaacaaagt
     5101 caacgccaac gcaatcgcca atgcatagag aaaattgatg atcggactta acagaatcga
     5161 attcgttttg atcatcgcat agcgcgtgtc taaatactcg ttgttcactt gttcaaattc
     5221 tttagctaaa cgagcttctt ggcgaaactg ctggataata cgcatgccgg aaatcgattc
     5281 attcaatttc gtgttcagct gacttaattt ttcccgcata tttcggtaaa tacgagaact
     5341 gaatttctgg taataccaga tgacgattcc taaaatcggc aagaacacca aagtccacaa
     5401 ggcaatttct ccattgattt gaaacatggc aacgaaagaa gaaacgactg caaagatccc
     5461 agtcaatacc atcaaaaaga cataccaaaa ttcaaatagc gtttctgtgt cattcgtcac
     5521 tcgagaaacg gtcgatcctg ccggtgtctg gtcaaaataa cgcataccta gtgtatgcag
     5581 cttttcaaat aatcttaccc gaacatactg ataggtcttc aatgaagcca tggaatacaa
     5641 ataccattgg aaaaaccaaa tgatactttt gactatgact ccaaacaagt acacaccagc
     5701 aaaaaacaaa ataacttgtg tcgtagctga ctgtttagcc agatgatcat ccataaaagt
     5761 ttggatgatc cttggcaata ggacattgat cacagacagg gcaaacgcaa ataagatagc
     5821 aacgatgaac gttcttctaa aaggtttggc gaacttcatc agtcgtttga tgatcttctt
     5881 ctgttcttta agtggaatcg attttgtcca ttccgattga taattttcat ccatcaggcc
     5941 tcgcctcctt ctatcttcgc ttctaattgc tgtttttccc acattcgttc ataccagcca
     6001 cctaatgcaa gcagctcgtg gtgggttcct cgttcaatga ttttcccttg atcgacaaca
     6061 atgatttctt ttgcatgcat cacgctgctt aaacggtgag ctgcaatgat ggttgttttc
     6121 tcttttctta tttcttttaa attatgcaag atagcttctt ctgttttggc atctactgca
     6181 gaaagtgcat catctaagat cagcaattca ggatttttga tcaatgctcg agcaatcgaa
     6241 atcctttgtt tctgtccgcc agataaagag actccacgtt ctccaactaa ggtatcataa
     6301 ccttttggca attccttgat ttcattgttg ataaatgcta aagaagccgc atgctctact
     6361 tcttcttctg aaagttttgg attagaaaaa cggatattat ctcgaatggt catcgaaaac
     6421 aggaagtgat cctgaggcac ataaccgatt gatcctaata aagcattcaa tgaatattct
     6481 ttgatcgaac gtccgccaaa ttggattttc ccttgataat catcgtattc acgcatcagt
     6541 aatttgatga ttgtcgtttt tcccgcacca gttttcccaa caatacctaa tgtttctcct
     6601 tcttggatcg caaatttgat ttgttctaat gctggtgatt cttctttcgg atagttaaaa
     6661 gaggaaatag ctacattcag ctcgcctttt gccggggtct gcactgcacc cagttgttcc
     6721 acgatatgcg tcttttcatg caaaagttca ttaacgcgat cataagaagc atttcctctt
     6781 tccaatacgt tgaacaatcg gccaatcgca aacatcggcc aaacaagcat accgatataa
     6841 ctaataaatg agaccatctg cccaatcgag atcgtgcctt ccaagacata ctttccacct
     6901 aaaataattg ttgcaacata agacagaccg ataatcaagg taatgaatgg atcaaataag
     6961 gagtctaaaa aattgactcg tttgttttta ataattgcat cttcaatctt ttcttcaaaa
     7021 tccgcgagat cttctttttc ttgtccaaat gttttaatga ctttgatccc agtgatactt
     7081 tcttgtgtct tatcattgat actagaaaaa gcttcttgcg aatcacgaaa agcatcgtgg
     7141 agttttgacc ccagcactcg tgaagtaacg gctagcaaag gcagaggaat cagcgcaatc
     7201 agtgttaagc gccaatcaat aaacaaaatc atcgcaatga tcgttgttcc gcccgtaatc
     7261 actgaatctg caaaggtcaa gattccagct cctgctacat tctggattgc attcaaatca
     7321 ttcgttgcat gagccatcaa atctccagtc cgatattttt ggtaaaagag attgtccatt
     7381 tctgtaaaat gtttgaatag ctgctgccga agttctttct ccaaccgagc tgcacttccc
     7441 caaatattcg tccgccagat ataacgaaaa agatattgta agaaagctgc aaggatcaat
     7501 agtaagatcc accaagaaat gatctttatt gataaattct tatcggcgat ctcatcaatg
     7561 atgatcccaa tcacttttgg aggaactaac tgtaccaagg ccactgcgaa tagggagaaa
     7621 atcccaatta aataatgttt cttttcctga cgaaaaaacc aacctaattt tctaaaaatc
     7681 gacattttac tcctcctttg tctttatcaa gagcatagga ttgtctgtcc ttgtcttttt
     7741 tgctgaatag tctgaatagt ctaacaaatt acgaaataaa aaacggactg aaaaaatcag
     7801 cccgcaaaaa gtctttgggt cagaacctat tttttccctt ggttcttcat ttgctgcatc
     7861 atctggcgaa ttttcttctc agatggtttt tgccccattg acatcatcat tgatcgcaac
     7921 atgtcctcat taacgggagg atttttttct aaatagtctt tcatatattt acgcgctagg
     7981 aaaaagccac ctactgcgcc gattagcaaa gcaataatag caataagtac gacaatacct
     8041 gttgatacca tataaatctc tcctttctat ttccgaattg ctctcaatga tttattttac
     8101 taaagtttat tgaaaaaaga aaggcgtatt ctttagtttc atgcaaaaaa tagtggtttt
     8161 tttagagaag aacaaaattt ctttcattaa ccagcaagac acgccacgaa aaaaaattag
     8221 gcataccgaa tctttttctt taattttttc tttctgaggt gtagaatgta atcgtagata
     8281 aataggaaag gtggtttttt cttgaataat caatccaaca aacaatttca taagcatcga
     8341 cttattgaac atacaaatgg tttatcttta gctgaagtaa atggaacagt ggatgtacca
     8401 aagggaaaaa gtttttttaa aacacttctt gcttactctg gaccaggtgc attagtagca
     8461 gttggttata tggatccagg taactggtcc acttcgatca ctggtggaca aaatttccaa
     8521 tatttattga tgtcagttat tttgatgtct agtctaattg ctatgctttt acagtatatg
     8581 gcagcaaaat taggaatcgt gacccaaatg gatttggccc aagcaatccg agcacggaca
     8641 agtaagacat tgggtgtcat tttatggatc ttgactgaat tagccattat ggcaacagac
     8701 atcgcagaag tcattggtgc agcaatcgcc ctctacttac tttttgatat tccattggta
     8761 attgccgtat ttatcactgt gttagatgtt ttcgtccttt tattgctgac aaaagtcggg
     8821 tttcgtaaaa tcgaagcttt ggttgtctgc ttgattttcg tcattttatt cgttttcgtc
     8881 tatcaagttg gcttgtctaa ccctgattgg cacagtttat ttgctggctt tatccctaac
     8941 agtcatacat tctcagactc acctgtcata ggtggtgaaa ctccgctgac tggagcgtta
     9001 gggattattg gtgcaacagt catgccgcat aacttatatc ttcattcagc agtttctcaa
     9061 actcggaaga tcgatcacca agacgaagaa gatgtagcac aagcggtacg cttttctact
     9121 tgggactcta atatccagtt gtcaatggct tttttagtca atgcactgct attgatcatg
     9181 ggtgtcgctg tattcaaaac cggttctgtc caagatcctt ccttttttgg tctgtatgaa
     9241 gctttatctg atccatcagc tatgagcaat ggtatcttga ttgctgtagc aaaatctggt
     9301 atcttgtcta ctttatttgc tgtagcactt cttgcttctg gccagaactc tactattact
     9361 ggtacgttga ccggtcaagt catcatggaa ggattcatcc atatgcgtat cccgatttgg
     9421 ctaagaagac tagtcacacg attgctttct gtgattcctg tattgatttg tgttttgttt
     9481 acaagtacaa aaggaacgat cgaagaacat accgctttga ataacttgat gaatgaatcc
     9541 caagtttttc tggcttttgc attgccattc tcgatgattc ctttactgat gatgaccaat
     9601 agccaagcgg agatggggag ccgattcaaa aattcatttg ttgtaaaact tcttggctgg
     9661 ttctctgtga ttagtctgac ttacttgaac ttacgaggat taccaggtca aatcgaaagt
     9721 ttcttcggcg agcaagccac ctcagctcaa ctagcattgg ctgatcaaat cgcctatgta
     9781 atcattgttc ttgtccttct cctgttggca tggacgattg gtgatttata taaaggaaac
     9841 aaaaaatatg aacagcgttt gcaagaaacg atagaagaaa tcgaatagaa actgattctt
     9901 ttacacaaag gaggttgtga cagaagtggg cagcttcaag aaataagaag gaatttaaga
     9961 aaattgcttt tcaaattttt gtgaaatttc ggcttatttc cgaagaagtt acttctggaa
    10021 caccgtgtat cagaggttgt gaaaaagctg ccctgcatca agtaataaga acagccaaac
    10081 aaaaatagtt cctcatattt tacgtttggt tgagcttatt accgcagatg caagcttttg
    10141 agcaccgttt attcggaaaa aggaactgcg acaagacttt tgtcacagtt ccctttttat
    10201 ttggaagata aagctttttt cagattcaat ttactaaaaa ctagcatgct tgttactgcg
    10261 atgaattgcg ttaatggcac gcctgcgcca gtgtagacac ttaaaaaata atcctttttt
    10321 tctggcgcat aagaacaaaa ccacgagaca ccgaaaagaa aaaccaattg tgtcagtatt
    10381 aatccgatga ctacaatcaa agaggaacta taataggcac gatcagcttt tgcaaatacg
    10441 gttcctagta tcataagtaa tatccctgct attgaagcaa tcagcacggg aatggccaga
    10501 acttggtaaa tttttatcat gacattcgcg acttttgctg tttttgtcaa ctggaaatca
    10561 ggtgttctcg gatcactgct atcgatccaa ttatccatta gtggaagatt caataattct
    10621 tgtgcctgtt cgacttgttc ttgcgtccca aaactataat tccccccttg atggaaatct
    10681 ttgtatcgcc atgcataagc atatcctgat ttcagaaaat caaatacgat tggcagatca
    10741 ttcatcgtct ttccatcccc tgatcccatc agatagaatt cttttttctt tttgatagtt
    10801 cccttttgat aggcttcttc taattcttga ttgacttttt tccaaaaatc attggtttct
    10861 tttgcattgt tttggtaata accagattcg cttaccacat ctcgcaatgc ccaaaaaatg
    10921 atatctcctg gtaattcttt tacttttccg ccttgggtcc acgcatgagt cgtatacaat
    10981 tcgtcaatac gagtagaaac agtatgaaat gtaggagatg cgtctaaagc tttatttaat
    11041 gcagcatgcg agacccatat ctcactgttt tctttattta agtttgtttc gtcatctatt
    11101 cggatcagct gtttcatcaa acgtccaaat tcgccactcg aacggtcatt gatggctgca
    11161 ataccatatg ttgtctcgtt tttatattcg atccactgat agacgatgga gaaaccgact
    11221 agagggagta aaaacaatga aagtttcagc cattcttgtt tcgaaaggag aaatggtcgt
    11281 tcttttaact tctttgcttc tttgaaagaa aagtcttgtc ctagcaagat ctgaacgaaa
    11341 ctgatgccta acgcgactac agtaaaagga agaagccaga tggaatcttc gcgaatgaac
    11401 caaaagaaag gaaaagaaac agctagccct aaaaaccacg gcagcaattt tttgctggaa
    11461 ttatatttgc ggtaataaat agctagtaaa caagcgataa tgatctcaac agtcgggatc
    11521 acgagagcat tacgatatgt tcgagtggaa tattcatgtg taaaactaat cggagaaaac
    11581 aaaaaataaa aatagatact taaaagaaga tacttgttct tcacgattgg tttaaacgct
    11641 agtgcaacca atatactggc aagaatactg aacatgccga ataaaactgc ataagaaaga
    11701 tgcagtttat gtgcaagtac taaaaacaag ctgtaggaaa tcccttttga aagtgtcgta
    11761 gtcgtgtact cccctagcca tttaccatcc aaaatatttt gagcatagtg aatcagcagc
    11821 tgatcatcat aggtcgccga catatctgtt ccatagattg ctttactata taaatacaac
    11881 cgaataattg taaataatac ggctatgcca agaataaagt atgtatgttt cttttttagt
    11941 tggtcgatca aggggtttaa aatgttcagc atcttttcgt cctttcaatc acgttttgct
    12001 acttgtttgt gaaacttttc gttttttact tttctatcag tataccctgt ttgtctgaat
    12061 ttaaaaagag ggggcaaaag gagcatggcg aaaacgaatg caaaaagcac tcctttgttt
    12121 cttaaacaaa aaaggacaag gcagactgcc caatcctttt ttgttgctgt caggtagaaa
    12181 acttaaagtg tgacttcttc tgctaataac tcagggaaca cttcgggatc gaatgcttgc
    12241 gctttgaaca tggaaatttc ttttccatat ggtgcgtaag ctttcttttt atctttaccg
    12301 acatatgggg tttctaatat tttcgaaatg ttgtcaaaca ttggatcatg gacgattttg
    12361 ttcaatgtgt caaatccaat tgttccaaaa ccgatattgg catgccgatc tttatgagaa
    12421 ccttgaggat ttttagaatc attgatatgc agtactttaa gccgatctaa tccgatgacc
    12481 ttatcaaact cttccattac acctgaaaaa tcttctttta tattatatcc ggcatcattc
    12541 gtatgacatg tatcaaaagt aacggataat ttttcattca attttactcc atcgatgatt
    12601 tgcgctaatt cctcaaatgt tcgaccgatt tccgtacctt tccctgccat cgtctccaaa
    12661 gcgatctgtg ccgtctgttc ttttgtcaaa acttcattca acccctcaat aattctagca
    12721 atcccagctt ctgcccctgc tcctacatga gcgcctgggt gaagggtgat ttgagtagca
    12781 cctaaagctt ccgcacggac aatctcttct ctcagaaatt gtacagcaaa tggaaacatt
    12841 tctggtttga tcgtatttcc caaattaatg atataaggcg catgaacgac gatatttgtt
    12901 agtccgtttt ctgccataaa tgcttttccg gcatcaatat tcatctcttc gatagctttt
    12961 cttctagtat tttgaggagc gcctgtatag atcataaagg tcgaagatcc gtaactggct
    13021 gcttcttctg ccgcgcctaa cagcattttc tttccgctca tgctgacgtg tgagcctaat
    13081 aacatggatg gattcctcct tgaaaggcaa ttgtttcgaa caattcctgt atattgataa
    13141 gatgatgtac aaagaaaata gtttgttttt ctttgccctg ttctccatag taactgaaac
    13201 tttccctaaa gaaaagctta agaactcttt cataaaaaag atcatttcct cacgttttat
    13261 ctcattcgaa aaagaaattc ctgtcaaaaa cagccttgtc tgtttttttg tcctattatt
    13321 tgctataatt agcgaaataa aaccattagt tgaggtgacc cttttgccac gcaaaaatac
    13381 tcgaaaaaaa cagaatagaa gaaaaaaaga aaaatggttt gttcctaaga ttatttttag
    13441 agtgtttcaa tcattgactg tttttattac tgttcttctt ttgttgttcg cagctctagg
    13501 tatcggaata ggtgctggct actttgccta tcttgtcgaa gacacacaat tgccgactaa
    13561 aaaagaactg cagacagaac taggaaacat tacggaaacg tcaaagattg tttatgcaga
    13621 taatacagag atatcaaaaa tccagacaga tctgatgcgg acgactattt catctgataa
    13681 gatttcgcct ttattgaaaa ccgcgattat cagtacagaa gatgaatact tcgataaaca
    13741 ccaaggttat gtccctaaag ctgttttacg agcactagtt tcagaagcga ctggcatcgg
    13801 ctcgtctgga ggatccactt tgacacagca attagtcaaa caacagatcc taaccgatga
    13861 aacaacattc aaacgtaaag caaacgagat cgttctttct gcacaagtag aaaaacactt
    13921 ttcaaaagat gagatcatta ctacgtattt gaatgtctca ccatttggtc ggaataataa
    13981 ggggcaaaat attgctggag tccaggaagc tgctagagga atctttggtg tagatgcaaa
    14041 ggatgtgact ctgccgcaag ctgcttatat cgctggtttg cctcaaagcc ctatcacgta
    14101 tagtccatac acgaatacag gcgcgctaaa agaagatctt tctgccggtc ttgccagaaa
    14161 agattttgtt ttattcagta tgtaccgtga aggacaaatt acgaaagaac aatacgaaga
    14221 agcgaaagcc tatgatctta caaaagactt tctcccacaa caaatcgcgg aacagactga
    14281 cagagaattc ttgtattata ccgtgatgaa cgaagcaaca aggattattg cacaacaatt
    14341 ggcagaaaaa gataatgctg atatgtcaga ttcatcggtc agtgaagctt attatcaaaa
    14401 agcacagcaa acgatccaaa acaaaggata tacgatccat tcgacgattg ataaagatat
    14461 ctatgctgca ttgcaaaccg gcgtagaaaa ttatggctac atgttagatg atggtgctgg
    14521 tagccaagtg gagactggaa atgtattgat ggataacaga accggacgta tctatggctt
    14581 cgtgggtggt cggaattaca gccagaatca aaataaccac gcctttgata cagaacgtca
    14641 agctggttca tccatcaaac cggttcttgt ttacggtcct gcaatcgata tgggacttgt
    14701 cggatcagaa tcccgtgtat cagattatgc aacgacttgg caagaaggaa aaaatgctgg
    14761 tgagaaaatc gtcaatgcga ccaatgaagg gtcgaacacc ttccaaaccg tccgtgagtc
    14821 actggaatgg tccaacaata tcccagctta tcacttatat caagatgtgt tgaattccgg
    14881 tggatcgaaa caatacgctt acgagcacta cttggcgaag atgaactacc cagcaaatga
    14941 taattggggc gttgaatcgg ctcctttagg gacagtagat gtaacgacgc ttcaacagac
    15001 aaatggattc caggccttag caaatggtgg agtctacgaa gaaggctata tcattgattc
    15061 catcacagat aatgcaggta atgtgattta taaacatgaa agtaattctg tccgtattta
    15121 ttccgaagca acggcatcga tcatgaatga catgatgcgt agtgtgatca atgaaaaaat
    15181 cactacacca ttcaaagatg tcatctcaag cttaaacgga aatctaggaa atgcagattg
    15241 ggtcggaaaa actggttcta ccaatgaata ccgagattca tggctgatcg tctcaactcc
    15301 atctatcact atcagtagtt gggcaggaca cgatgacaat actggaatgg acagccaagc
    15361 aagaatacgt tcggctaatt atcttgccaa tctaatcaat caagcttacc aagcaaaccc
    15421 tgagattttc ggcactgatg agaaatttga cttatcatcc gatgtgatga agaaaaaagt
    15481 atctgggttc acaggacaaa cagctggaaa agttactgtc aataaaaaat ctatccaaac
    15541 gccaggaaaa acagtggaat cactttgggc aaaaaatggt ccgaagaaaa gcgcctttaa
    15601 attcggcgtt ggcggtactg atgaaaacta tacagattac tggaacaccg cttcagctta
    15661 tgctagagcg aatccagaga ccaaagaatc agacgataaa gatgattgat ctaatagatt
    15721 aatcagaatt aaagggagct gcgacaaaaa tcttgtcaca gctccctttt tccgaataaa
    15781 tggtgttcaa aagcttacat ctacgatatt aagacaaatt tatgtaaaaa tgtgaaaaac
    15841 cattttcaca aaatttttct atataacttt actaacgaaa ctgttttaat ttcttttctt
    15901 gtctttggaa taattcggtt agtttttctg caaattgccc ttttactgat tcttttaagt
    15961 gtgcatgtcg gttagaaaat tcttgagtag cttcttctgc agtcatttgg tggttttgat
    16021 ctgtcaatcg tttttctgcc caagtttgct gagatatccc cataccaact gaacgtacat
    16081 ttgatcgatg tagatttttc tctaattctt ttagtaattg cattaaaaat tcattcataa
    16141 gatgatttct ccatagttat ttgtctgatt ttttgacgat aggaacgttc tagttcgtct
    16201 tgctgctgat agagttttcg tcgttctact tgtagggctt gttgataatg ctcctgataa
    16261 ttcgaataac ttcgtcttgc ttgaaacaga ggctcattat ctaaagctaa gttctttaat
    16321 acatgatgca gtcgctgctc ctcttgctcc atcaatcgta atccttgatt cttaaagtgg
    16381 atcaattcat cttcttgagc ttctaattgt cttcgttcac ttcgataatc tctttccaat
    16441 tcttctagtt tcatctactt ctcctttaat tgaattgctg tgctaattct cgatctcgtg
    16501 ccacgatttc attgattttg tcttggatct ccttcgccaa tgtatcaaag gcctccccca
    16561 tacgtcttgc cttattgatt cgtagcagat agacttcttt cgcagaatcg actaagttct
    16621 cttttgtggc gcccacacta gctaatgctt ctatgatttc ctgttcagtc aacgaagggg
    16681 cgacaacacg tgcttgccgc aagccttctt gccatttttt ctctagctca aaaattcctc
    16741 tttggttgat gcggataact tcttccatag cgcttttcat ttttaccgat aacttttcca
    16801 ccactaacct agcttgtgtg tcatctaagt aaatttgttc attaactgtc agccggccgc
    16861 cacttaatcg tagtttttta cgtatgttgt tcagttcttg caactgatag gaaaacaact
    16921 gggtcgcttg gttttctttc tgttgttttc gagctttaac attgttttca ttttcaggaa
    16981 taatcagctt tccattttca tcaaacttcc aagaggttaa actgtgggaa gcaagcagat
    17041 tcaagtatgc tgtttccata ctgatccgaa cttcagcacc tgtgccatta ccaccaaaat
    17101 taccgataaa atcggcgtga tttcggtaat tggtgagcat accgggattt tcttccaccc
    17161 atttttttgc ttctggtgat aagagatcat acgggtccgg cccgttgaag ccgacatttt
    17221 tccattgttt ctctgctgct acatataatg ctaagaatcc acctaatgaa tggccggtag
    17281 tagaaatagc agcttctcca tattcctttt ttacttcatt agcaaatgca atcgctgaat
    17341 tgatttgccc cgctgttttc aatccactaa cggataaaat cttgtttgtt gttgcttttt
    17401 cagaaaaact tgctcctggt ctattcccat agagattcat aacacttctt gcatccgttt
    17461 ccaaatcttt ccaatcgctt aagttcgttc ctgcaaaagc aatcactact tgagaaatat
    17521 ccactttacc gttcttgatt ggcgctacag ccatcgcctg catgccgtcg aaggagtttt
    17581 tgatttttag gatttggaat tttgtttctc caaagttttt aatggcgcct tctttgtaac
    17641 ttttactata ttgaggatgt tttggatcta gccaatatac tttattactt attcctttat
    17701 actgcttatc agtaattgtc atactttttc ctcactccca tctgtataga taactataat
    17761 ctcatcaacc gtaatccctt ctttttgtac ttcttcattt tcaacaccat agccatctat
    17821 ctcattgctt tcttttgtaa agctataatc aaaataaacg gcttttcctt ctgtattggt
    17881 catcgttatc aatgcttcat agattcctgt catgtcgttt ttctcaaatt gttcgatttt
    17941 cacttctttg atatccgcaa aggtattttt cagaactttg acggactgtt tttctactgc
    18001 gagtaaatct tgctgttttt gcgcttctct tttgtccatg taaaattttc ctccgattcc
    18061 tatagtaaga atggtcaaaa tgatgactat ggtctttcgt cttttcttag gcaaatagaa
    18121 gtctcctttc gggttatgta tgatggattg tttctccttt aagataagta cgcaatcgag
    18181 cctggatttt tttggatttt ttagagttac ttttctactt ttctactttc ttttttatca
    18241 cgttttcatt ttaaagtaat tttgatagta gtgaacaaaa tgtagcatct acgcacagat
    18301 tgtgtcgtat acgatggacg taaggtttgt ttccgccatt ctctcttttt tttaaagtca
    18361 caaaaaaagg ggttatgaca aaattttgtc ataacccctt ttctcgtata aaggttgttc
    18421 cagaagttga ccactttggg aacaaccttt aaatgattga tcttttatta tttagttgat
    18481 cctttttctt cgattccata aggcaagata atcgtttttt cttcgacttc ttctttattc
    18541 atcattttcg tcaataggcg catggatacc gctcctaaat cgtataatgg ttgtgtgata
    18601 cttgtcaaac gaggacgagc tacttctgtc agtaaagaat tgttgcttgt gatgatttcg
    18661 aattcttctg gtacttttac gccgcggtct aataaaccat ccaagatacc aatggctaat
    18721 tcatcgtctg aaacatagac agctgttgca ccgctattat ggatccggtc aactagagac
    18781 agaccatctt taaagttgta ttgtgcctca aaaatcaatc cttcgctata tggtagattg
    18841 ttttcgctta atgcttcttt atagcctttt agacggtttt gaccattgat aggatcaatc
    18901 agcgcaccgc tgacaaaagc gattttttta ttgccgcttt ttgctaattt atttactgca
    18961 tcttttgttg ctgatacata atcaatgttt acgctaccta cttgttcatc tggatcaatg
    19021 gaaccagcta gtacaacagg cgtttttgaa cgtgaaaatt ctccacggat ttcgtctgtg
    19081 atatggtgac ccataaagat cacgccgtcc acttgcttag ccaataaatt attcaataca
    19141 ttgacttctt tttgatcgtt gccatctgag tttgctaaga tgatattgta tttgtacatt
    19201 gttgccacat cgtcgatacc acgtgctaaa gaggcaaaga acatattgct gacatcagga
    19261 atgattaccc caactgttgt tgttttctta cttgccaagc cacgtgcgac tgcatttgga
    19321 cgataatcca agcgatcgat aacttccaag actttttttc ttgttgctgg tttgacgttt
    19381 ggattgccat ttacgacacg tgaaacagta gccatcgaaa catttgcctc tctcgctaca
    19441 tcatagatag tgattgtttg cttttccatt tgtgttctcc tattcatatt ttatatgttt
    19501 tctgaaaata cgtcttacat ttctaaaata ggatatcaaa taatctcaac aattgcaacc
    19561 gttttatctc tgttttcatg aaaattttta cttttttcag ttttaagaac gttggtcttt
    19621 taatcaatca gtggtacaat tattgaatac aaaattaatt tagaaagtag gttgccaaat
    19681 gaataacgta aaaatcaatg aactacgtca atggatggca caacaccaat tagaattggc
    19741 ttatatcagc gaccctggcc acatcgctta cttttccgga tatgaaagtg atcctcacga
    19801 gcgtgtacta gctcttttta tccctctgga acaagatcct ttccttttca ctcctgctct
    19861 ggaagttgaa gatgctcaaa acagcagctg ggcgtacgat gtacgtggct acttagacag
    19921 cgaaaatcct tgggagatca tcggaaaaga gcttcgcgca cgctatgggc aacctggtaa
    19981 tgtaggtatt gaaaagaatg ctctgactgt cgatcgcttc gatgctatct ctgatatctt
    20041 gacaggtaca gattcttaca ccgatctaac ttctattatc cagcgtctgc aactgatcaa
    20101 aacggtagaa gaaaaagaca aactgatcga agctggtaaa tgggccgatg ttgcctttga
    20161 aattggcttc aaaacagtaa aagaaggcgt aactgaacaa gcaattatcg cagaaatcga
    20221 ataccaatta aaaaaacaag gtgtttcaca aatgtctttc gacacattag tccttgcagg
    20281 agcaaatgct gcaagccctc atggtacacc aggaagcacg aaggtttcac caaatgaact
    20341 agtgttattc gatcttggcg ttatttggaa tggctattgc agtgatgcta ctcgtacagt
    20401 tgcttatcaa aaaccaactg aattccaaga aaaaatctat aacatcactc ttgaagcaca
    20461 gcttgccgca caagaggcag ttcgcccagg tgtgacagca ggtgaacttg atcaaatcgc
    20521 acgaaacgtg atcaacagct atggctacgg cgaatatttc aatcaccgtt taggtcacgg
    20581 aatcggaaca accgtccacg aattcccttc tcttgtagaa ggaaacgact tagtgattga
    20641 agaagggatg tgcttctctc ttgaaccagg aatctatatt cctgaaaaag taggggtacg
    20701 tatcgaagat tgtgtgtatg tgacaagtga tggttgtgtg ccattcacga ctacaccaaa
    20761 agaattactt gttttagagt aatgaaaata aaaaaggtga gagctggtcc gctctcacct
    20821 tttttattct agaaatgagt aatcttagtg attggtagtc gaatctgtca ttgtatcttt
    20881 catttctttt ttagcttctt ttgcatcttc ttttgcatct tcttttgcca ttttcaacat
    20941 ttcttttgtg tcatctgcaa ctggttttgc ttctgacatt ccttcattga ccgtctctcc
    21001 tgctttatct gcagcatctt ttacatcgat catgatatct tctgaagtat ctttagcaat
    21061 actacctaat tcgtctgctg gctttttcag atcttctgct gtttttttga aacgattaga
    21121 taaatcagct gattgttctt tgaggtttaa caagatatca tctgattcgc ttcctgcttc
    21181 ctctgctgat tcacttacct tctgcttaac attgcctgct agatcccctg cttgttgaga
    21241 aagatcttgc gctttttctt ttgcaatacc tgttaactca gatgattttt cttttgccat
    21301 atctgtgtaa tctgaagcca tgtccatcaa atcatctgct tgagcagcca attcatcccg
    21361 aagttcttta ccagatttgg gtgctaacaa taatgcggcg attgctgcgg ctgtacctcc
    21421 aactactgca cctaataaaa atccaccttt tttagccatt cttattttcc tcctgtcgtt
    21481 tcagtatgtt ttttgttttt aaaaatttca tcgctgttcc accgactttt ccggcaacag
    21541 tcgctttagc tgtcgttttt ccgattccgc cgactttcgt cacgatgttt ttactagaat
    21601 gattaagttc cgatacactt tcacttaaat cagcaactgc tgtgaacaat ggatcgatcg
    21661 ttgcgacttt tccattgata tccaagagca gctcattact tttgactagc aagccttcta
    21721 cttgtctaga taaaagatct acgtcttgtg tgacgatctc aattgtcgta tttgcttctg
    21781 cgacagtctg gtctactttt tctaaagttt tggaaacttg caacaatacg cgaacgatga
    21841 aaacgacaag tatcgcaaag gcgattgctg cgatcaatcc tgcaatttct cctcctgaca
    21901 tgatcaaact cccttcttgt ctctcttttc tataaggata gcattttcgt gttttttaaa
    21961 caaataaaaa cgagcttcaa ctacttttta ttcaattagc agctgaagct cgttagaaaa
    22021 aaagattatt ttttcttttc ttctttgatt tctgttattc cttttggatt atgattgatc
    22081 ttgcttcctt ctgcttctaa tgttttacct aaagacatga tgtattcacg caaaggtgcc
    22141 cctacttctg gatgtgtcag accatattca atacttgttt tcataaaacc aaatttgtca
    22201 ccaacgtcat aacgtttacc tgtgaattta cgggcaaata cgcgttgtgt tttattcaac
    22261 gtatcgattg cgtcagttag ttggatctca tttccagcgc ctggttgttg ttctgataat
    22321 acatcaaaga tttctggagt caataagtaa cgcccaataa tagctaaatc acttggtgct
    22381 ttgcttggat ctggtttttc aacaaagttt ttgacattgt ataacccatc ttccagtact
    22441 tcccctgggt tgatgattcc gtattttgaa gtttcatcgt gaggaacttg cataacggca
    22501 atcgtagaag cgtgtgtacg ctcatagtca tcgatcaatt gctgtgtcaa tggtacttcg
    22561 tcttccatga tatcatcgcc tagcataacg acaaacggtt catttccaat aaaggctctt
    22621 gcttgaagaa cggcgtgtcc taaccccttt ggatgagatt gacggatgaa atgcaaattg
    22681 acatctgtcg tttcttctac taatttcaat aattcggttt ttcctttttc acttaggttt
    22741 gtttctaatt cgatatttga atcaaagtga tcttcgattg gtcgtttagc ttttcctgta
    22801 acgatcaaga tgtcttcgat tcctgatttc aacgcttctt caacgatgaa ttggatcgtt
    22861 ggtttgtcca cgattggcaa catttccttt gccatcgcct ttgttgctgg taaaaatctt
    22921 gttcctaatc ctgctgctgg aataactgct ttttttactt tcatatacat tcgctccttc
    22981 aaaaaattgt taatgagaat tcattttctt ttttcccatc acgtaacatg atttcttttg
    23041 cggctgtttt gatttctttt tgttcataca gcacttcata gattgtttga gtgatgggca
    23101 tttcgatttc catcatttgg gctagttcca tcgctgcttg agtagtattc acaccttcaa
    23161 caaccattcc catattggct agcacctctt ctagaggctg accttgtcct aacaagttgc
    23221 ctgcgcgcca gtttctggaa tgaacacttg tgcaagtaac gactagatca cctactccgc
    23281 ttaagccaat aaaggtcagc gggttggcac ccattgctac acctaaacgg ctgatctcag
    23341 ctaatccgcg cgtcatgatt gccgcttttg cattatcgcc aaaccctaag ccgtgaatag
    23401 caccagctcc aatggcaata atatttttca aagctgctcc catctctaca ccaatcacat
    23461 cagggttggt atagattcga aaataatggt tcatgaacag ctcttgtaca tattttgctg
    23521 cttttacatt tgtgcttgcc gctgtgatcg tcgtaatgtc atgctttgct acttcttccg
    23581 catggcttgg cccagaaaga acagcaacag cttttctttt ttctgtcggg atttcctcta
    23641 acaagatttc agagattctt ttatgcgttc cctgttctaa tcccttactt gcgtggatga
    23701 tcactggctt cgtattcaat ttggcaacta gttcttttgc aacagaacgt atcgctttcg
    23761 tcgggataac gaatagtacc gcatctgcat cttttactgc atcttccata tcggtgaatg
    23821 ctcggatgga atcgggtaga tgatgatctg gtaaataacg ttgattggtg tgttgggtgt
    23881 tgatttcttc tacttgtgaa gcacgatgtc cccaaatccg aacgtcatgt ccgttttcag
    23941 cgaggacttg tgctaaagca gtcccccaag agcctggtcc taaaaccgct actctataat
    24001 tcattttacc actcctgaaa tttttttcat ttttaacgct ctgaaaatca aataatttgt
    24061 tgattttttc ctaatgagcg gtcataatcc ggtaaacttc ctttttttct acgccaaata
    24121 aacagaatca atgtgccgac aaataaaaca gcagacaata attgagaaat gcggatcaca
    24181 cctaaaaagt aaaggctgtc tgtgcgtaac ccttcgataa agaaacgtcc gaaactatac
    24241 cacattagat aacataaaag gacttctcct tttttcaaaa gatgtggttt cttccgcaaa
    24301 accaaaagca gaataaatcc aatcaaactc caaagtgatt cataaagaaa ggttggttgt
    24361 cgatagattc cttcgatata catgttatca atgatgaatt gcggtaaatg cagactttct
    24421 aagaaacttc gtgtcgtgtc tggaccatac gcttcgtggt tcatgaaatt tccccatcgt
    24481 ccgattgcct gcgccaataa tacgcttggt gcagcgatat ctaaaaaggt ccacaaagaa
    24541 ataaaatgat atttggtaaa aaagtacagt gtgattgcac ctgcaatcaa tcccccgtaa
    24601 atcgctaatc ctcctgaacg gatattgaat atctggattg gatcagctat atattgggga
    24661 agatcgaata aaacataata caagcgggca ccaataatgg aaatcggcaa tccccacaac
    24721 ataaaatctg tcacgtcatc tgcttttaat cctacccgaa cagcttctct gctgctgagc
    24781 cacatggcaa taatgattcc tgatacgata atcaatgcat accagtagat aggaattcct
    24841 acaagctcaa aagcaactcg attgatttgt gctaacatat tgtcactcct tgctagtcat
    24901 tgcctgagtt ttcttcaatc aagcgactta atctttcttc aaatgttttc gttgcgtcaa
    24961 agcccattgt acgtgcgcgg aaattcattg cggctacttc aatgatgatt gcaacgtttc
    25021 gtcctgtttt gacagggatt tttatttgtg gaatgcccac ttccgcgatt tcaacggtcg
    25081 tgtcttcaga gcccagacgg tcgtatttct tatctttcgc ccatgcttct agataaacag
    25141 ctaactggac ttgcatagag cctcgtaccg cactcgcacc gaaaagattc attacatcta
    25201 tgataccgat ccctcgaatc tcaatcagat gttccaggat tttaggaggt tctccgatta
    25261 ccgtcagttc atcttgctga tagacatcta ctcgatcgtc tgcgatcaat cgatgtccgc
    25321 gcttaatcag ttccaacgcc gtttcacttt tccctatccc actgtcccct tgaattagta
    25381 cacctaatcc gtagacatcg actaacacac cgtggacact tgttcgaggg gctaagcgtc
    25441 catctaaata actagataat tcacctagca gccgggacgt tgagataggc gaacgcagca
    25501 cagctatccc attttcattg gctgcttgga tcatttcttc cggagccggc aatcctcttg
    25561 aaataataaa tgcgggcata tcttcactac ataatcttct cataatgatc aaacgctctt
    25621 ctgggatcat ccgttccgca aaactgattt ctttactgcc aaatagctgt aaacgattat
    25681 gggaataata attaaaataa ccggtcaact ctagtcctgg gcgtgagatg tccccagtca
    25741 cgatgacgcg gtctaaactt ttctcgtcgc ctgtgacgat ttccaaagat aattttttta
    25801 ctaattggct aacttttaca acttctgaca ttcatgctac ctcatttcta ttagagtgtt
    25861 ctctatcatt ttatcattgt ctagccaaaa gtactaccaa ttaaaaaaaa cgtttttatt
    25921 taattaccaa tgtaatagaa aacgcattct ttattctaaa caaaaaa
//
LOCUS       DAIGOK010000028        25702 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.028, whole genome shotgun sequence.
ACCESSION   DAIGOK010000028 DAIGOK010000000
VERSION     DAIGOK010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.028"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            137..2197
                     /locus_tag="NOA01_000926"
     CDS             137..2197
                     /locus_tag="NOA01_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HCI0632138.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2280..2849
                     /locus_tag="NOA01_000927"
     CDS             2280..2849
                     /locus_tag="NOA01_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCI0632139.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3024..3830)
                     /locus_tag="NOA01_000928"
     CDS             complement(3024..3830)
                     /locus_tag="NOA01_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632140.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3968..5482
                     /locus_tag="NOA01_000929"
     CDS             3968..5482
                     /locus_tag="NOA01_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HCI0632141.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5885..6868)
                     /locus_tag="NOA01_000930"
     CDS             complement(5885..6868)
                     /locus_tag="NOA01_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HCI0632142.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6873..7520)
                     /locus_tag="NOA01_000931"
     CDS             complement(6873..7520)
                     /locus_tag="NOA01_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCI0632143.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7605..8237)
                     /locus_tag="NOA01_000932"
     CDS             complement(7605..8237)
                     /locus_tag="NOA01_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCI0632144.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8253..8804)
                     /gene="coaC"
                     /locus_tag="NOA01_000933"
     CDS             complement(8253..8804)
                     /gene="coaC"
                     /locus_tag="NOA01_000933"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HCI0632145.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8801..9538)
                     /locus_tag="NOA01_000934"
     CDS             complement(8801..9538)
                     /locus_tag="NOA01_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HCI0632146.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9535..10524)
                     /locus_tag="NOA01_000935"
     CDS             complement(9535..10524)
                     /locus_tag="NOA01_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCI0632147.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10782..11324
                     /locus_tag="NOA01_000936"
     CDS             10782..11324
                     /locus_tag="NOA01_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCI0632148.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11341..12426
                     /locus_tag="NOA01_000937"
     CDS             11341..12426
                     /locus_tag="NOA01_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0632149.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12438..13244
                     /locus_tag="NOA01_000938"
     CDS             12438..13244
                     /locus_tag="NOA01_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI0632150.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13245..14072
                     /locus_tag="NOA01_000939"
     CDS             13245..14072
                     /locus_tag="NOA01_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI0632151.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14069..15142
                     /locus_tag="NOA01_000940"
     CDS             14069..15142
                     /locus_tag="NOA01_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCI0632152.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15570..18266
                     /locus_tag="NOA01_000941"
     CDS             15570..18266
                     /locus_tag="NOA01_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCI0632153.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18343..18831
                     /locus_tag="NOA01_000942"
     CDS             18343..18831
                     /locus_tag="NOA01_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCI0632154.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            18843..19337
                     /locus_tag="NOA01_000943"
     CDS             18843..19337
                     /locus_tag="NOA01_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCI0632155.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19358..20167
                     /locus_tag="NOA01_000944"
     CDS             19358..20167
                     /locus_tag="NOA01_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCI0632156.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20164..20985
                     /locus_tag="NOA01_000945"
     CDS             20164..20985
                     /locus_tag="NOA01_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCI0632157.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21036..23135
                     /locus_tag="NOA01_000946"
     CDS             21036..23135
                     /locus_tag="NOA01_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HCI0632158.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23132..24250
                     /locus_tag="NOA01_000947"
     CDS             23132..24250
                     /locus_tag="NOA01_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HCI0632159.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24289..25167
                     /locus_tag="NOA01_000948"
     CDS             24289..25167
                     /locus_tag="NOA01_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCI0632160.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25313..25510
                     /locus_tag="NOA01_000949"
     CDS             25313..25510
                     /locus_tag="NOA01_000949"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HCI0632161.1"
                     /translation="MLLRFLFSFCCALYTLRIFTGGVHLNSNILCYFMSVVIVIVAYQ
                     CMFIFRNVDFIILGLMFEFFW"
BASE COUNT         8129 a         4469 c         5426 g         7678 t
ORIGIN      
        1 tttcttgaaa caataaaaaa tcaaaaaaaa tactctccta atagtccatc ttcttttgat
       61 tttatttata taatgaaatc atcttatgaa aattactact tcatgtttag acaagagtag
      121 ggaggactaa gaataaatgt gttcaaaaag acgttcaaaa caactaaata aacacgcttg
      181 tcgtttagat aaggaacggt cgtgttctga aaaacactac gttaagttta tgatgcgcac
      241 gatgctgatc agtttatctg taggtgtcta tatgcatgta cccgttcagg caacgacaca
      301 aaccatcaag gtcgtacgta cctctgtttc tgagaccact gattccccta tcactattac
      361 tcatactttc actgatgcaa atggaaaaca gctacttcct gcattgactt cctataataa
      421 agtcagttat cctccaacga taaaccacta ctggttaatt cctaacaaaa cattatggac
      481 atcggaaacc ggaagcagtg tgccgctttc tatcttttcc gatatgactg atcctacaaa
      541 aggtgatcag ttaaaacaga tcactgatat tttgaatcat gctttgacag atataaagga
      601 cggtggaagc gcacaagttt cttatgtata tgaaaaagat tattcacagt ttgaaagaaa
      661 gaaaatcgat gttctggcag ggacaaaagt ttcgatatct gatctggtag aaaggctgaa
      721 gaacgcagat ggaacacaag gagatatgtc caaagtaatt tcaaacaatg ggaatatcgt
      781 ggatacgact aatacaggaa catctactat ttccttgtcc tattttgatc caattgcttt
      841 aaaaacaatg actgcatctg ctgtgatcca tgttcgtgct gatcaaacag ctatcaaagg
      901 aaaagatctt cacttaagaa caggagagag ttacgcccct aaagatcttg ttgaatatgt
      961 aacaaaaagc gatggaactt caggaaataa agaagaaatc aaaatgaacg ggacagtaga
     1021 tactacaact ccgggagaat atcccgttgt attaacttat atagactcga aaacatcaaa
     1081 tgtggcagaa acaacagctg tagtttatgt agaagcatct gctgcttcat cgactgtcat
     1141 cactcatttt atagatagtc aaacaaataa agaacttcaa gcagaaacac atatgagtag
     1201 tgtaaatcgt attcctgatt tgcgcctacc tgctgaattt tctgatcgag tgccaaatat
     1261 agatttatcg acttatgaac aaaatgggaa agtcacttct atttctgatt ggatgcaagc
     1321 aaacggatta gacagaaaaa gtcactggtc ggagatcctt gaagtacttc actcagaact
     1381 gatgaataat aggaagcaat caggagggga aattaccttt acttatgttt atataccaga
     1441 caatcgttca ttagaaggtc atgatcttat tgttcatcca aacgaaaaaa taaacaaaga
     1501 agagttgatc caatcagcaa caaattcctt aggtaaaccc gtaccgttaa aggatgtatc
     1561 ggttacaatc aatgaagtac cattggagga tggttatttg accaaacaaa caggtgattt
     1621 tacgattatg tatagttatc atgacccgta ttcactccaa gaactccatg ctcagtcact
     1681 attacatgtt ttaccattaa acaaacagat agatcaaaaa gaacaaaaaa gacaagaaga
     1741 aagcttgtca gaaaaaaaac aacaaatggc tcatgcagtt tctccaacag ggaaagtaaa
     1801 acaaacaaga agcctagagt tagcaagaat atctagaaaa atctcagaat ctaataaaac
     1861 gataaggcaa gaagctcatt tggccccaca agtttctgga ttggaaaatg aaattttaga
     1921 gcctaaaata aaacaacaat cagaaaaaga atttgttgct aaacagtcgg aagatcagaa
     1981 ggaatcagat gaccagaagg aaacgaagaa aaaacgcttg tcaactattg attccaccga
     2041 tgagcatctg cagccgcaag taccacttcc tagtccggga acacccgcac ctggtggagg
     2101 tattccttct tctgctttag gagatttcat gcgaggtatg gcaggaacat gggtatacgc
     2161 taatcgaaat gaatttgacg cagatgactg gttctgattt atatctctat ctgttatagt
     2221 caaataagta gctgagtcat atgttatttg gcataatgga gacgaggagg gatagaaaaa
     2281 tgttagataa aacgttacct tacgcagaaa tctggatgac aagaccactt caacaaaatg
     2341 ttccaatcac gcctttagcg gaaggttttg aatttcgatt ttatcaaaaa gaagatgaat
     2401 ctgcttgggc ggagattgag acttccgttg gggaatttca aacgacagat gaagcacttg
     2461 agtattttca acaaaacttt gctccttatc ctgaagaact tgaaagaagg atgttttttg
     2521 tagagacaaa aaaaggagaa aaagtagcaa cctgtaccgc ttggcgaaaa aaatcaagag
     2581 agcaagtgta tccggttttt cattggttag cagtgaaacc agattaccaa ggaaaagggc
     2641 tcgcaaaagc attgactgcc agagtcttgc aagaatttcc taccctccat acggacggac
     2701 caatctatct tcacacacag acatggagcc atcaggcgat tgctctttat aaaaaaatgg
     2761 gttttacttt tattgctgaa aatctagacg gcacacctaa tccagattat gaaaaagtta
     2821 tgcaggtttt aagtaaatta gataaataac gaaatggtga aataagtcct cagtattggg
     2881 ataaaaaacg aaatagtcat tcctcatatc ttactttttt gagttaatac tatcggataa
     2941 ctaatggcat gccatacatt caaaaaagaa ggggcagtga caaaagtatt gtcactgccc
     3001 cttctcttgt tttttagcat gttttatcgt ttgatttttt taggaataaa attcttgatg
     3061 aacgtaaatt tttggtaatc gtttcgacta tagaggtaac caaatacact gaaaacaact
     3121 gctccaataa aattaacaaa taaatccttc atagtatcca tgattcccac gtctaagtaa
     3181 ccattttgga tgattgtttt atctgtttta ccatctgcat tttgactttc aatgattgtc
     3241 cgagtgatat ctggaacatg atagacagtg tttccgtctt tacttaactc cacactattg
     3301 atttgctgga tgatcgtatc tttttgcata tcgaattgca gtaatttatc tgctgaaaat
     3361 tccacaaact cccacataac accaactgtc atcgaaaaac agaaggaaac aactgctagt
     3421 aataaaggag tcaagttcat ggtttttgta tttttattga gcaaataaac aagggaaaag
     3481 ccgacacccc cagctaaaaa tccgctgaat gcatgtagaa aagtatccca cattgaaaaa
     3541 tagccataaa aatcacttaa ctctcctaaa actgttgaac taaaaataaa agataacaaa
     3601 atgatttcta gcatatttgg aatctgtatc tttaatatct tttccagaaa ataagggatt
     3661 tcaaataaaa ttaatgtcag taataaaagc attgtatttc cccagtgttg gttccagacc
     3721 tgatccacta gcataataac gacgatgata cgtaataaaa tatagattcc gtttgtgatt
     3781 ctacctgcag tttttgtact gttcgtaaaa aaggttttca aatgattcat aaaataactc
     3841 cttcatgtat atccttaatt atactaagaa gcgtgtgttt accaaagcta tatgacttaa
     3901 agaaaaacag agttgatatt gtgaaaaaaa tcacttaata tagtataagg agaaaagggg
     3961 gatacaaatg aaaaaattag ttgctggttt attaacagcc gttgcacttc ttgctatgag
     4021 cgcatgcggc gcagatcaga aaaatacagc gcagacaagt tctagtacga aggaatctgc
     4081 cgaagtatca tcaggcgctt ctgaacaagt atatacagat ccaaaagaaa tgaaagaatc
     4141 ttatgatgtc gtgattattg gagctggtgg tgcagggatg gcagcagcta tcgaagcaaa
     4201 agatgctggg ttgaatccag taattttgga aaagatgcct gttgccggag gaaacacatc
     4261 taaatcatca agcggtatga atgcttcaga aacaaaattt caaaaagaag aaggaatcaa
     4321 agactcgaat gatgccttct atgaagaaac gctaaaaggt ggtggaggaa cgaatgataa
     4381 agaaatgctt cgatactttg tcgatcactc tgctgatgcg gtcgattggc tagatcaaaa
     4441 cggtatcaaa ctggacaact taacgatcac tggagggatg agtgaaaaga ggacacatcg
     4501 tccgtctgat ggctcggcta ttggtggata cttagtggag gggttattga ataacgtcca
     4561 tgagcgggaa atccctattt ttgtcaacgc agatgtgaca gacattaaga aaaatgggga
     4621 aacggtcaat gaagtgactg tacatgttca aggagaagaa ccaaaagagg tcaccggaaa
     4681 agcaatcgta gttaccacag gcggttttgg tgccagcaaa gaatttattg aaaaatatcg
     4741 tcccgattta gctgattata ttacgacaaa tcaagaagga tcaactggag atggtatcac
     4801 catgattgaa aaattaggtg gacaaaccgt tgatatggat aaaatacaaa ttcatccaac
     4861 tgtgcagcag gatgaggggg tcttgattgg tgaggttgtt cgcggagaag gagcaatctt
     4921 ggtcgatcaa gaggggaaaa gatttgtcaa tgaaatggat acaagggata aagtatctgc
     4981 agcaattaca gcattgcctg aaaaatcagc ttatttgatt tttgaccaag gcgtgcgtga
     5041 tcgtgccaca gctattgaat tttatgacca aaaaggatat gtcacaactg gaaagacaat
     5101 ggaagaatta gcagaaaaaa tcgatttacc aaaagaaaca ctagcaaaaa cagttgatgg
     5161 atggaatacg gatgttgcgg agaaaaaaga tgctcaattc aaccgtgcca cagccatgga
     5221 acatgactta tcgtctccga attattatgc gatcaaaatc gcaccaggaa tccattacac
     5281 gatgggcggt gtgaaaatca atacgaacac tgaagtatta gataaagaaa accagccaat
     5341 tagggggtta tatgctgctg gtgaagtcac aggtggctta catggaaaca accggatcgg
     5401 tggaaattca gttgcggaga tcattatttt tgggcgacaa gcgggcatac aagcagctcg
     5461 atttgcttcg gaaaatgaat gaaatagaac gtaaataaaa agaggttgtg aaatcagcgt
     5521 tttgagctaa taccgagggt cagctgattg aacactgttt agtgaagagg ttgtgaaaaa
     5581 gctgccctgc atcaagtaat aagaacagcc aagtaaaaac agcttcttat gtttttaact
     5641 tggttggact tattaccgca gatgcaagct tttgaacacc gtttattcgg aaaaagagcg
     5701 tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa cacgtataaa cggcggaagc
     5761 agaagaaatt ccttcggaaa taagctgaaa ttcacaaaaa tttgaaaagc aattttcgtg
     5821 aatttcttct tatttcttgg agttaaacgc ttctgtccca gcctcttttt tgatcagcat
     5881 gattttattc agttatcttc aaaatgattt ttccgatgtt tttgtttgct tccatatatt
     5941 catgcgcttg tttgactgcg ctgaattgga atgtacggtc aatgattggc cgaatcttgc
     6001 cattttctag aaaaggccca gctttttgca taaaagtttg tgcgagctct gctttatact
     6061 gatcacttct aggtgtcaat aatgtaccaa gcagttggac acgtttccca atcaatacac
     6121 caaggtctat ctcaggcacg atggtcccac ctagcatacc aatcaagacc caacgtccat
     6181 caacggctat agaagctaaa ttcttttccc agtaagaagc gccgatgaaa tccaaaataa
     6241 catcgactcc ccgaccttca gtgatttttc gtacctcttc ttgaaagtct tgttttttat
     6301 agttgatcaa ttcatctgca ccaagttctt tacaaaaggc gagtttttct tctgatcctg
     6361 cagtaacgat gattttcgcg tgagaaagct gtctagctaa ttggatcgct gctgttccca
     6421 caccacttgc tcctgcatgg atcaagacag tttcgccttc ttgtaatttt ccaagccagt
     6481 aaagtgtttg ataagcagtc aaaaaaactt ctgagattcc agcagcagaa acatagtcta
     6541 gcgtatcagg gagaaggatt gcacgatcgg caggcatcac agcatattcg gcataaccac
     6601 ctagattgac taatccgtat acttttgtcc caggagtaaa agtggagata tcacttttat
     6661 tctcaactac ctctccagct acttcgactc ccaaaatagg gtagggaggt ttcaaagaga
     6721 ctgattctct tttcataata tctgttcgat tgacagctgc tgtatggact ttgatcagta
     6781 attcgccttg ttttggttta ggaacaggca cttcaatttg ttctaataca tctacagaac
     6841 caggatgctt gattgaaatg gcacgcatgg atctattcct cctttgccca ttcttggaat
     6901 gctgtattca actctttgct accttcagaa agtactagag tcgtacgaga aatatccgtt
     6961 tcttccacta gagcggcaag tacagcagca actgtagagc gagtgatcgt ttttgatgcc
     7021 ttctcattag gcttagttaa ttgaatggaa gtaacttctt catcgttagt caatgtgact
     7081 ggttgaagaa tgacaaaatc taagttagtt cgtttcagcc attcatctgc ataatgttta
     7141 gtaatataat aatctgtcat ggactcaggc cattttgctg gttcatctgc ataaacagca
     7201 ctgaccatca gatagcgaga gacatgcgca ttttcagctg cgatcactgt tttgattgcg
     7261 ccatccaagt ctacttgcag aagattcttt ccttgagagc cggcagcaaa aatcaataca
     7321 tcgatgcctt tgaatgcttc ggtcattttt tccacggacc aagttaaatc aaaagagaca
     7381 tacgaaacat tctctttttt tactaatgtt tgggaagcaa catctctcac ccctgcaaaa
     7441 atttcatggg aacttggagc taaatctttt atcaggtgtc ggccaatttg tccgtttgca
     7501 cctacaacaa aaattttcat ttggatcatc ctttcgacgt caaatctttc cattcaatag
     7561 taacaccaaa agagaaaaaa atcagagatc ggctacgatg actatcagtt agtaagagaa
     7621 gggtgcttga agtatcttaa caagacattg catactgctg cagtcgcaag acctgcgacg
     7681 attccttcag gtataccact tgtaaaaata accgctagaa tagctgaata aacagcattt
     7741 aaatttgttc cgatgacttt ggcataatct tgctggaata aaaagtagat catattcatc
     7801 actaaaatcg tattggtcaa tgaaccggaa agtccagcta agaataaaga aacggaatct
     7861 gctttttgtt tcattagttt gcgcagacct ttataaacga aataaggaac caccccaatc
     7921 aagatgcgag gaataaacgc gatcagcaag gctttccagc ttccatgttc tgttccgatg
     7981 actgggataa acggagaaaa gacaaaagac aaaggtgttt ggataactgt gctacggatc
     8041 atgctgatca gtccaaatgt accgccaaga aaagcaccta gtcttggtcc taaaacgatc
     8101 gaagcaatga ttacaggaat atgcatggtt gttgcattga ttggaccgat agggataaaa
     8161 cctaaaggcg taacagctaa taaaatcaaa atagctaaaa acatcgcagt taatgtaaag
     8221 ttttttgtat ttttcataag tactcctttt ttctaagcat ttctttcatt aagagtcagc
     8281 atgacctgtt cgatgatcgt ctgaatatca gctaaggcac cttttccaaa atctccacaa
     8341 gctaaaagag attctcttgg ttcaatttcc tgatagccga cagatctcaa tgtttccaga
     8401 ttacgctgag tgatcgggtt ttgatacata ttcgtgttca tggcaggggc aatcaatttt
     8461 ggtgttccca ctggaagcgc cattgcgact gttgaaagaa gatcgtctgc aatgccgttt
     8521 gctagcttgc cgataatgtt tgctgtagca ggcgcgatga gaaacaagtc agcttgtttt
     8581 gctagttcga tatgattgat tacctgcgga tttgattctt gcatgacgtc tgtatggact
     8641 gcgcgcttcg acagagattg caaggttaat ggcgtgataa aatgtgtgct gcttttagac
     8701 ataatcacat ctacctgggc acctaatttg acgaattgac ttgtaagatc agcgcttttg
     8761 taagcagaaa tacttccagt aacgcctagt aagatctttt tcattttgat tcctccagtc
     8821 taattttctc agtaatcaat gcagcaatct cagatttact ttgagcttct tccactgttc
     8881 catctttaga taagagatag ccgtggtgtg tctcgccatg gatttccgtc aaatcatttg
     8941 cgaaaataaa atcagttttg ttatttatca gactattttt tgcgactgcc agaagagatt
     9001 cctttggtac atctactaat agtttgaaac caatgatttt tgcttttggc tgccattcac
     9061 gtagataaga aatgattttt ggtgttttct tcaaaacgag cacaagatgc tcggtgttag
     9121 aagagatctt tgaaacagtg gtatctgtct gttcattttc cgcaaaccaa ttgtccagtt
     9181 cttcttgcgt tgaagaagtg ggtaggtttt ttgccagctg ttcttctgaa aaactaaaag
     9241 caggagtaaa atcactgact gccatactgt ggataatcgc atcgtagtgt tcttttttcg
     9301 ttagttcttc taattgtaaa aataagtcca gtgcagattc gatcgtataa atcgttatat
     9361 tggagtgtct tttcggtttc agtgcttgtt tcgtagtcac gtaatcgatt gtggtatccg
     9421 gattggcagc taaataatca gctaatgctt gccctaatcg tcctgtcgaa tgatttgtaa
     9481 tagcacgtac ttgatcgatt ttttcagtcg ttcctccagc tgtgattaga atcttcattt
     9541 atggtcacct acatttacta gtgttcttcc ttggtgtgtt ccagcaagta gtttagatgc
     9601 tgtttcaggc aattgatcca gagtaatctc ttggacgacc agttcatctg caatctgcaa
     9661 atccgccatc tgttgccata aagaaagacg ttgatctatt ggtacattga cagaatcaat
     9721 tccaatcaac tggatgtttc gcagaataaa aggcaagaca gtcgcgttta gtttgattcc
     9781 cccagcattg ccacataaag ctactgcacc gttataagag atcaatggaa gaagactgct
     9841 tagctgctcg ccacctactg tgtcgatgac atagtcgatt tgctgttttc ctaaagcttt
     9901 tgttgtctct ggcagaaatt catcaggtcg tacgacttgc aaagcaccta gactttttag
     9961 ccatgtgaca gactcttctt ttctggaaaa ggcaatgatt gactggaatc ctagtttatg
    10021 taacagggct atcgctgtac tgcctacacc gccagaagca ccagtaacga caactttttt
    10081 atcggaggtc ataccttgac ggattaaagc gttcacacac aacatagcag taaaccctgc
    10141 tgtaccaagg atcatcgctt gacgcagcgt catatttttt ggtagaggaa ctaaccagtc
    10201 tcctggtact ttttgatatt ggctataacc gcctggatgg gtgacgccaa gcccaaaacc
    10261 agtagcaagt acaagatctt cttttgaaaa acgtggattg ttactttgga cgacagttcc
    10321 ggataaatca atccctggag tcatcggata tgttcgtatc acctgaccag actcgctcat
    10381 tgccaatgca tctttataat tgacatcaga ataggcaatt tttaccaaga cttcattttc
    10441 agccaactca gtaaatggca gatcactgat tgctgtatgg aaaacaggtt cctttattac
    10501 ctgaaaagat cgaaaagcgt tcattcgtga gacactcctt ttaaaatttt cttttatcat
    10561 accataaagc ctactctttt attcttgttc ttccgtaaat ttagacaaaa attaggtgaa
    10621 aaataaagaa tcaaacaact agaatttatt ccaaaagata aagaaatatt cagcaaaaaa
    10681 aattttacaa agggttgcct ttttcaataa atttcgtata ctaatacgag tggtagtaaa
    10741 caggaagttt actattagaa aacttcgatt cggaggaaat tatggaaatt ggtgagaagt
    10801 taagaaattt gcgcatccaa aagaatttga cacaagaaga actaggcgaa cgcacagatt
    10861 tgtcaaaagg gtatatttcc cagttggaac gagatttaag ttcaccatca atggaaacat
    10921 tcttttctat tcttgaagtc ttaggtgtga ctccagaaga attttttcat caagaagcag
    10981 cgaatctgca agtggtttat tcaaaagaag atcatacggt ttatttcgat gaagaaaacg
    11041 ggtatgaatt ggaatggctc gtggctgatt cgaatgaaaa agaaatggag cctgtcttgc
    11101 tgacttttga aggtgcagga gaatataaga cgtttgaacc ttcattatct gaaacgttta
    11161 tttttgtatt agaaggggaa ttagagttaa gcttaggaga gacggtctat gtggcaaaag
    11221 caggacagtc gatctattat caagcgacaa aacaacatca attaaggaat catctcaaaa
    11281 aacgaacaaa agtattgatt gttgcaacgc aatcatattt gtaatatagg gggtaaaaac
    11341 gtggggaaac caatcatttc atttgatcaa gtagtgaaac gctatgatga tgaaacagta
    11401 ttaaaaaaag taagttttga gatcgaacaa ggaaaatttt atacactgct tggtccttcc
    11461 ggctgtggta aaacaacgat cctacgtatt atagctggat tcaccgaagc tacagaaggg
    11521 gacatctatt ttgaagggaa acggttgaat gatttgccag ctaataagcg acaagtaaat
    11581 acagtcttcc aagattatgc actttttcct catatgaatg tttttgataa cgttgctttt
    11641 ggattgaaga tcaagaaaat gtccaaatca gatatcgaaa agaaggtaaa agatgctttg
    11701 cgtatggtcc agcttcctgg atacgaaaaa cgggagatta gtgaaatgtc tggtggtcaa
    11761 agacagcgtg tagcgattgc acgagcaatt gtaaatgaac cgaaagtatt gctattagat
    11821 gaaccgttat cggcgcttga tttgaaacta cgtacagata tgcagtatga actgcgggat
    11881 ctgcaacaac gattaggaat cacgtttatt tttgttactc atgaccaaga agaagcgttg
    11941 gcaatgagtg atgagatttt tgtcatgaat aaagggaaaa tcgtccaaag tggtacaccc
    12001 gtagatattt atgatgagcc aatcaatcat ttcgttgctg attttgttgg ggagagcaat
    12061 atcgtggacg gtgtaatgat cgaagataat ctagttgagt ttgttggtaa gcaatttgaa
    12121 tgtgtcgatg gcgggatgcg tctaaatgaa ccagttgaag ttgttttacg cccagaggat
    12181 ttaacgatca cgacaccaga taaaggaaaa ctagtcgtaa cggtcgatac tcagctgttt
    12241 cgtggtgtcc attatgaaat catctgttat gatgaacagc aaaatgaatg gatggtccat
    12301 tcaacgaaaa aagctaaaga aggaagcaaa gtaggattgg cctttgagcc agaagatatc
    12361 catgtcatgc gtttcaacga gtctgaagaa gaattcgatg cacgtcttga cagctacgaa
    12421 gaataggggg gacaagaatg aaaacaatgc gcaggatcta ttccgtccct tatattttgt
    12481 ggctcggttt attcgtgatc gcgcctgtat taatgatcat ttatcaatct ttttttgata
    12541 tgaatgggca attcacgctg aataattatc tatcttattt tacgtcgggg acatcgcttt
    12601 ctatgacgat caattctgta tggtatgcat ttttgatcac gtttttcacg ctgatcatca
    12661 gttacccgac tgcttatttt ttaacaaaac tgaagcacaa gcaattatgg ctgatgctga
    12721 ttattttgcc tacatgggtg aatctgctgc ttaaagctta cgcatttatt gggattttca
    12781 gtgtgcacgg cagcgtgaat catttctttg attttatcgg gatcgggccg aaacagatat
    12841 tattcacaga cttcagtttt ctgttcgtgg caacgtatat cgagattcca tttatgatta
    12901 tgccaatttt caatgcattg gaagagatga atccttcttt gatcagtgct agtagagatt
    12961 taggcgcgaa tagctttgag accttccgac gagtgatttt tccgttatca ttaaatggtg
    13021 tgaaaagtgg tgtccaagca gtatttattc cttctctttc tttattcatg ctgactcgtc
    13081 tgattggcgg aaatcgggtg atcactttag gaacggctat cgaacagcat ttcttggtta
    13141 cacaaaactg ggggatgggt tctacaatcg gtgtgatttt aatcattgct atgttcatcg
    13201 tgatgctcct gacaggcgag aagaaaaaag gaggtcgctc ataaatgaaa aaatttaaat
    13261 ggtcgtatct ttatttaggg atcgtctttt tattcttata tgcaccgatc ttctatctga
    13321 ttttttattc cttcaatgaa ggaggaacga tgaacagctt tacaggattt acttgggaaa
    13381 attatgaagc tgtttttgaa gatacacgtt tgatcattat cgtattgaat acgttcatgc
    13441 tggctttctt atctgcattg atcgccacga tcatcgggac atttggggct atgggaatct
    13501 attacacgaa aaaacgacgt gcacggacag cgttgttgag catgaataac atcttattag
    13561 tctctcctga tgttattatc ggtgctagtt tcttgatttt ctttacacta ctaggattcg
    13621 gattaggttt tcagagtgta ttgctttccc atattgcttt cagcattcca atcgtcgtct
    13681 tgatggtctt gccaaaattg caggaaatga acgattcaat ggtcgatgcg gctagagacc
    13741 ttggtgctaa taatttccaa gtagtcaaaa atatcatttt gccttatctt tctccaggaa
    13801 tcatagctgg atattttatg gcatttacct attctcttga tgattttgcg gttacatttt
    13861 tcgtcacagg aaatggcttt acgacgctct cagtagagat ctattctcgt gcaagacaag
    13921 gaatcagttt agaaatcaat gcgctaagtg cgttagtttt cttgttctcc atgatattgg
    13981 tcattggata ctactttatt agtaaagaaa accgtccaaa acggctgaag aaaaacagaa
    14041 gagtacgcga gggggtggca cggctgcgat gaaaaaatta caatctctgt ttttaggtat
    14101 tctcatcatt attgcggtgc tgctgcttgg tgttcgtcaa cttgaaaaaa caagtggcat
    14161 gagtggagca aaggtactta ccatctataa ctggggagat tatatcgatc ctagtttgat
    14221 cacgaaattt gaaaaagaat atggatacaa agtgaattat gaaacatttg attctaatga
    14281 agcgatgttc acaaaaatcc agcaaggtgg gacaaactac gacatcgcta taccaagtga
    14341 atatatgatc caaaaaatga tcaaagaaaa actagtactt ccattagatc attcgaagat
    14401 caaaggatta aataatattg atccacgatt tttagacttg gattttgatc cagataatca
    14461 gtattcgatt ccatattttt ggggaacttt agggatcgta tataacgata aattctttga
    14521 tgcaaatcaa attaagcatt gggatgattt gtggaagccg gaattaaaag acagtttgat
    14581 gctgattgac ggtgcacgag aagttatggg gctttctttg aatagtttag gctattcttt
    14641 gaatagtaag aatatggcac agctgacaga agcagcaaat aaattgaatc atttaacacc
    14701 aaatgtcaaa gcaatcgtgg cagatgagat caaaatgtat atgatcaatg aagaagcctc
    14761 cgtggcagtg acattttctg gcgaagctgc cgatatgatg tgggaaaatg aacatttaca
    14821 ttatgtgatc ccaccagaag gatccaatct ttggtttgat aatatcgtta tgccaaaaac
    14881 cgtgaaaaat aaagaaggtg cctatgattt catcaacttt atgctagaac cggaaaatgc
    14941 ggcacaaaat gcagagtata ttggttattc tacaccaaat gaagaagcaa aagcgttatt
    15001 gccaaaagac atttcgtcgg acgaacaatt ctatccatct gatgacacga tctcccatct
    15061 tgaagtctac aaagatctag gatcgaaata cctaggcatc tataatgacc tattcttaga
    15121 attcaagatg tataggagat aatggcaaaa ctcagtaaga atggggactt atcaacgttc
    15181 taaagagcta aaaatcgtcc tttttctatc tgagttttcc aatcttctac caaaagcgga
    15241 tgaaacgccg ctttttttgt gttattttat tttttctacc acgtttttct accagagaaa
    15301 agtggacaaa accattgtaa aatagggccc gagacgggct agtaaatcta agattgaaaa
    15361 agttacagaa ggataaagta attggaaact atgcaaatat acttaaccct tctgtaatct
    15421 attaataaaa ctaatatatg aatccagttc aatatttgta gctatgaaaa aacataaaaa
    15481 tcatattaga taaaaactaa attggtagat aaattatttt gtcagatatc tgaggcaaat
    15541 attcgactat ttgtgtttca aagtaaaaat tggaatacaa atatagaata gacatattgc
    15601 agccgatagt ttattatgct tttagacaca ttaaactgga ggaaaaaatg aaaaagagcg
    15661 ctaaagatcg agtttttgat gagctgatac gactaacagg gaatatgtct tctgtcactg
    15721 ccgagaaaat ttcgaagaat ctaggtatct ctcgtcagaa tgccagccat tatttgacga
    15781 gattagtgga agataaaaag gtcgagaaat taccgggaaa gcctgttttg tggaagcctt
    15841 tagatgaata tgcggttgta gataatacgg agcaaataaa cgaagccttt cattctgtag
    15901 ttggtcatga cggaagtctg agagaggtca ttcaaaaatg tatagccgct gtcaaatatc
    15961 cgcctaacgg actcagtgta ttaataaatg gagccacggg tgttggaaaa agttttttag
    16021 ccacaaaaat ttttgaatac gctatccatg agcaaattat tgaaaaagat gcaccatttg
    16081 ctatcttaaa ctgtgccgat tatgcggaca atcccgaact gctttcggca acactgtttg
    16141 gatacaagaa gggggccttt acgggggctg aaaaggatac cgatggttta ttggcaacgg
    16201 ccaacaacgg ctatcttttt ctcgatgagg tccatcgcct atcgaaagaa aatcaagaaa
    16261 agctctttct atttattgat actggaaact atcgtccagt cggtgaaaat gtaaattggc
    16321 attctgctaa ggttcgtttt attttcgcca cgacggaaaa aggtgaaaat tatttattag
    16381 acacctttga tcggcgtatt caaattagtg ttatgttgcc aacttttgaa gatcgcccca
    16441 ttcgagaacg attagagctg attcaattat tttttcaaaa tgaagcggat gttttacaaa
    16501 aagatattat tgtttcaaga gaagccttgt ctttaatgtt atctcatccc tttagtggga
    16561 atattggtaa gctgaagaat atcattaaaa tcagttgtgc ggatgtgtat agtcgaacac
    16621 aagaagaacc attaacgatt gggaaaaatg agatcttgat tcagttaaat atgctggaat
    16681 ccgaagtaga aagcttgccg attgctagtt tattgataaa gaaaaatcaa aagataaaaa
    16741 cggatcgaat caaaccgttt gattatgatt cgattttgga aaaaattact cagctgataa
    16801 atgaaggtaa tttcaatgaa aatttttctt cgataaagaa tgaagtacag catttggtgc
    16861 atcacattga taaggattct tttttatcaa tgagtagaaa attgatgatt cagatgttta
    16921 gaaaggtctg ggtatccgtt ttaggaaaaa cgtacggatt gacgacgagt ggacctgttg
    16981 cagaaattat tagcagatcc tatgtcattt ccccaagcta taagacagac ttaaccggga
    17041 taaatcgtac attagctgca aatctacctc ggacgactta cttatgtaat cagttcttgg
    17101 agcatttacc accgctctca gaaaaggacg agaactttct aagactgctt attgtcgtga
    17161 ctttatctga ttatgtagat gaatccatcg agctgaaggg cctgttgctt gctcacggag
    17221 atagtacggc tagcagcatt caagcagtag tgaatcaatt atgcggcaat tatgtttttg
    17281 aagcgttgga tatgccagta gagagtaatg tgtcagagat tgtcgaatcg acaaggaatt
    17341 tcgtgaaacg gcaagtgaat acagatagtc tagtgctgat cgtagatatg ggctctttaa
    17401 atcaaattta cacacagatc aagaacgatt taaaagggga gttacttctt ctgaataatt
    17461 tgacgacttc cattgcactt gatattggct tgaagatgac gatgaatgcg ccatttaaac
    17521 agattgcgga ggatgcagca gacaagtatt caatcaatgt tcaatacttt gaaggctttt
    17581 ctcaaagtag caacattatc atttcctgta tgtcgggttt ggggatttcg gacaaattga
    17641 aagatgtttt tcgtcatcat ttatcggatg aatccatcga agttttcacg aaggactaca
    17701 gagaactacg tgaattaatc gaccaaaatg atgaaactta ttttgacaag acaaaattgg
    17761 tcatcacgac ttcggatctt ccacgttcct tttcaattcc taatattaat gtttatgaca
    17821 tcttagacgg tgatggagca gccaatttgg agcatatttt gagtcctgaa atgaagccgg
    17881 cagaatttaa ccgcttgatt caggagctag taaaattctt ttcgttggaa ggaattgcgg
    17941 accgtttgaa ctttctgaac ccaacgatcg tgatgagtga agtggaaaca gtcatttcta
    18001 aatatgaaaa ttactatcat ttcactttaa atggccgtgt aaaactaaat ctctatatgc
    18061 atacggcatt gatgatggaa cgtttattct tggctcggac agaaaaatct gaagaaccga
    18121 attatgattt gaaaccagat gaagatgaat tttattatgt ttctagaagt atttttcaat
    18181 caatggagat gaaatataat tttaaagtca atgcttatga gctttcgctg ctctatgaat
    18241 tattaggacc gtatattcaa aaataaagtg tctaaaaaag tgtctaatct gtttttttta
    18301 ggttagacac tttttttaat gtgaattaac gtgattaaac caatgttttt aaaagttggc
    18361 acaggaattg cttatataaa agtgaacaga aagaaaggaa ttaaccatat gaagaaaatt
    18421 ctaatagcga ctcacggtca cttagcttca ggctttctta gcagtattca attactgacg
    18481 gggaaggttc aagagattac ggtgattaat gcttatgtgg atgagtgtga ttttgaacag
    18541 gagttgaatg tatttgtaaa ccaggtgaac gagcaggatc agatttttgt ttttaccgat
    18601 ttatttggcg gaagtgtcaa tcagaagatt accaaaacct ttttggagaa agagattgct
    18661 gtaactattt tgtctggctt taatctgcca attctgctcg aaatagtatt ggcaaccaag
    18721 gaattaacaa aggaggatat tcaggaactg gtaaataaat gccaacagga attaaagatg
    18781 accattcttg atgaggtaca tgtagtaaat gaagaggaag atttttttta ggaggaagaa
    18841 aaatgattaa agcagtgcga attgatgaac gattaattca tggacaagta gcgatgactt
    18901 ggacaaagag tctaaattta accgggttag tagtagcaag cgatgaggcg gctaccaacg
    18961 atattcaaaa gatgacattg aagatggctg caccatcgaa tgtcaaagtc attattaaaa
    19021 cagttgatga cgcgattcat ttgttaaaag atccgcgggc gaaggacatg cgcttgatgg
    19081 ttttagtccc taatgtcaaa gatgcggtaa aagttgcgaa agctttccca acggaaatcg
    19141 aattaatgaa cgtggggaat gctgggaaaa tgacaccgcc atcggaagat aagaaaacct
    19201 tatcaaagga agtgatgtta acgacgtcag aaattcaaga cttaaaagaa ttagttgaac
    19261 tttatccaga taccgttttt cagcctacac cggcgatgga aaaacgttca gctatctcta
    19321 ttttgagtga tttttagaaa aaggaaggag aaaaattatg tatgatgcat ttgtagtcgc
    19381 tttggccgtt tttattggag ttgctggaca tgaaatgttc gggatgtcca tgctgagtcg
    19441 tccgattgtt gttgctccgt tagcaggagc gttgatgggg gatttgcaga cggggttgat
    19501 tgtcggggcg tcactagaag ccatctttat gggtgtcgtc aatatcggaa tctctagtac
    19561 ggcggagcct tctttggctg ctgggctggc tacagcgttc gcaatcaaaa tgggaggaaa
    19621 cgtggatgcg attattccca ttgtctttcc actagcagta ttaggattac agttaatgaa
    19681 tatgattttt tcttttgtat gtggaccgat ggctgctaaa tttttcaaat ttgcaaaatc
    19741 tggtgatcaa aaaggaatca tcaatctgca cttctttatg tggtttgtcc actatgcgct
    19801 ttatgctctt attccattct ttgccgtttt atttggagca gatgttgtca aaagcgtctt
    19861 ggataatatt ccagaggtca tcatgaacgg attaacagtc gctggaaatc tgctgccggc
    19921 agttgggatg gcgatgctgt tacggatgct ttggagtaaa aatattgctg tgtggttttt
    19981 ctttggggcc gttgttatgg cgtatctcga gttaccattg attgcaattg ccgcagttgg
    20041 aacgattatt gcaattgtta tagcccaaag agatctacaa ctaaaaaata tatctgttca
    20101 tgcagttcaa agcgatgctg ctgtaaatgc aacgcttgac caagaagagg aggatttctt
    20161 tcaatgaaat tagctgatgg aatttctaaa gatgagaaaa aattggttcg gaagatgttt
    20221 tggcgctcga tgactatgta tgcttcggtg aatccggtga cgatgggcgg cggtggtttc
    20281 gcctattcta tgatgccctt cattaatcat ttctacaaaa gtgaagaaga tcgagctgcg
    20341 gcgctggaac gtcatacaca gtatttttcg acgaccattc cttgcgcgtc ctttgtcatg
    20401 gggattgcgg catcgatgga gaaggaaaat tctgaaaaag ctggttttga cgtggaaagt
    20461 atcaacgcga tcaaaacgag tttgatggga ccattggctg gaattggcga ttcattgttc
    20521 tggggagttt ggcgtgtcgt taccgcggga ctagcgatca acttagccaa tgccggaaat
    20581 attttggcac caattatctt tttagttatg ttcaatatac ctaattactt gatgcgatac
    20641 tatggcggcg ttttaggcta ttcattaggc tctaaatata ttgaaaagct gtatgcgaac
    20701 gggttaattg aaattctgac aaaagctgct agtatcttgg gtctgatcat ggtaggtgca
    20761 atgacgatca gtatggtcaa attcaatacc gttttgagct tttcaatgaa gggcgaagaa
    20821 ctaatgaatg tccaagaaat cctcgatagt atttttaaag gaattgtacc gttattggta
    20881 acattgagct gtttcaaatt actagataaa aaagtcggcg tcatcaaaat tatttttgga
    20941 atggtcgtct tgggaatttt attgtcatta cttggaatcg catagtagga agctgcagct
    21001 actaggctgc agttttttta gcaaggaggg aaaagatgac tattaatgaa gtaatttatt
    21061 cacccaaaca cccatttttt aatgctgagt taaacgacgg aaatgtgact acttcaaacg
    21121 aatacagcta ctatcggaat attcctgatg taaagggatt gaccttcgat gtaagtaatc
    21181 aaaagacatt agcaaacctt gatgtttgcg ggacgattca aacgttgacc ttttatcgag
    21241 aaaatttact aactgaggaa aaaccaggtg tttgggtcaa taaacagctc tctcaaacca
    21301 attcgttatc cttaaaggtt gaagtaaacg ggattgtcta ccatttgtca gaatcggatc
    21361 atcgtattga agtcgatgtg attcaagact gtttgcctag atatattcat cattattcga
    21421 cgtttaaggt cattgtagta tcctttgctc ccatactggc taataagcgt ttaagtatgc
    21481 ttgttcaaca ggtttacatt gtgaatgaaa cacacgaaag ccttcaagtt tcagtgaaag
    21541 atgtgccttc gtatcaagag aagtattcgg atcagcaaaa tgtgttgatt tctcaaaaag
    21601 gaaataagga cacgatcgca caacaggaag tggcaagctt cgccattgct tttgtggacc
    21661 caaatgcttt tgaagaagta atgacttttc aggaatctga tatcgaaaaa tggctgaagg
    21721 aaacattgaa ctattttgct cagctatttg ggcagctgtc aatgccaaac gatagagttg
    21781 tccatctcta tcgtcgtgca ctttatcaaa gcttttctag tttcggtatg aatcgacagg
    21841 gacaggtcgt cggcagcaat tggggcagct atcctgctac taatcgaatt tggaataagg
    21901 atatgtatta cgcatcattg ccattcacca tgtttgaacc ggagttatgt caaaaaacgg
    21961 ttctatggtt tgatcaatat ggcgttaagt ttcctggttc aaaatttaca ggaggtatca
    22021 atcattctct gagtaattcg ttatccagtg cgttactggc tagtctttat tttgaacata
    22081 ctggggatac aaaattcttt gagcggtgtc cgcaggttct actgaatgct tcaagaatta
    22141 ttgaggacat attagctcaa cgtcggtcag gggaaccaat gctttttcca tctgtttggt
    22201 tatcagacgc ctttgcactt ggaaagtatc ataccggcag caatctttgt ctttggaagg
    22261 cctgtattgg acttgcagaa atttttgaag ccctagaaca actgagtctt agcaagcgat
    22321 acaaaaacat tgcctgtaaa ctaaaggaat caatttgtaa acaaatgact atcgatggtc
    22381 cttttggtga gcaatttttg gagggaatcg gtgatgagga gaaaacaacc tattcagtga
    22441 agcactatca aaaaccgatt cttgagcaag ggttaatctt tttaagtgat gtgattgtgg
    22501 atggaaaaat taatttattg atgcatgatg gagaagaatc agacactacg ttgatgcctt
    22561 tttatcaatt tttagataat aaagatcatt tatatcagaa caccatggcc ttttctgcta
    22621 gttctttcaa tccaacctat agcgaggaaa tcaaaggcat cacttgggga ttagaatccg
    22681 gcgccacttt tcctggcttt atcactgttt taatgagcga gctgcacaac cctcaagctt
    22741 tcgtaacccg attagaagaa ctaatttgtt tggcagattt agacggttcg tggtggtggt
    22801 ggccgtatcg cttagaggct caacaaggag aagtcgtgcg agactttggc tgcggcaaat
    22861 gcggctgggc atcgggtctt tttgtcagcc taatgatcag tcaatatttc ggattgaaat
    22921 tcaaaaaagg agtattgcaa attgatcctg ttgataattt aacgttttct tggaagaatt
    22981 tgaagttcgg tcaagccttc atttcgattg aatgtacgca atccgagttg agtatttcaa
    23041 atttgtcaga aaagccattg aaaataagtg atacgaaaaa aattcttcac gtagaaaagg
    23101 gaaaaacgat tcacattcaa aggagaaaaa gatgagtata ctaaaaaata ttacccaatt
    23161 taaaaaaatc cagagtcgag caatcactcc cgaaaatcca acaggcgaga aaggtaaagc
    23221 tgcgatgttg ccaagcgagc taggaccaag ccgcaaaggt cgtggcgcaa ttccgctgac
    23281 ttctggtgaa gaaactgtga ttgcggatat tactggaacg ggaataattc gtcatatgtg
    23341 gatgacgatt cgtgatcgaa cagaatttgg cagttttgtt ttacgtgatt taatcttaag
    23401 aatttattgg gatgggtcgg atagccctgc tgttgaaagt cctttagggg atttcttttg
    23461 taatgggttt ggagagcgtt gcgaggtgaa ctcgttgccg atagtggtta atcctacagg
    23521 gggctttaat tcctatttcg aaatgccttt ccaaacatcc gctaaaatta cgatcgagaa
    23581 tttacatccg aaggatatgt cctcattttt ttatacgata aattacgctt tggtggacga
    23641 attggaagaa aatagcctat attttcacgc aaattggcat cgggaacgca ttaccaaatt
    23701 acaggaggat tataagttaa ttgatcgatt aaagggtcaa ggctattacg tgggtactta
    23761 ccttgcgctt acctgcctag aacgttattg gtggggcgaa ggcgagttta aattctacat
    23821 tgatggggat agtgagtatc cgaccgtaac ctcaacaggt tcagaagatt attttggcgg
    23881 ggcgtgggcc tttcatgagt acgatgagtt cggacgtcca catgcgaaaa actattcaac
    23941 agccttttta gggtatcctt accaatcaaa tagagatgcc acaagagatc gttttgaaac
    24001 gggaaaattg aatgccgttc atgcctttgg tgacgacgca atgccacgac atggtcttta
    24061 tcgttggcat cttcaagacc ccatctgttt taccgaagat ttatcggtca ctttacaaca
    24121 aatcggcaac gatgatgttc gcttatttga gcggcaagac gatgttgcta gtgtcgctta
    24181 ctggtattca acagaagctt caggaaatac taaacgtcat ttcactgatc gagaacgctt
    24241 gccgcgttaa tgaagtgttt ttcacatatt cgaagggagg aacctatgat gatatatggg
    24301 gcaattgaag ctggagggac taaatttgtc tgtgcagtcg gtactgagga attaacgatt
    24361 ttggaaagag tcagttttcc cacaacaact ccagttgaaa caatgaagca ggtcattgat
    24421 ttttttaaac cttttaagga acaattggca ggcatcgggg tcggctgctt tggtccaatt
    24481 gatattcaat gcaaatcaac gagtttcggt catatcacat caacaccaaa acttgcttgg
    24541 caaaatttca attttgtagg aacgctaaag caatatttca atattccgat tgaatggaca
    24601 acagatgtca atgcggcttg ctatggtgaa tatgttgctg gttctggcaa agggttagca
    24661 ggtgttgcat acttcactgt gggaacaggt atcggaggtg gtgccttgtt caatgggact
    24721 ttcgtggaag ggttcagtca tccggaaatg gggcatatgt tgatcaagcg acatccaaag
    24781 gacaattttt caggaaactg tcccttccac caagattgtt tagaggggat ggcagcagga
    24841 ccagcgattg aaaaacggtt gggtgtaaaa ggccagaatt tattggctga cgattcgttt
    24901 tggcagattg aagcctttta ccttgcccaa tgtgcataca atacaacact gatgttttcg
    24961 ccagatagaa ttatttttgg cggtggtgta atgaaacaag aacacatgaa gaaaaaagtt
    25021 caggataaat ttgttgagct tataaatgga tatgttgaaa taccaccaat tgatagctac
    25081 attattacgc ccgaattagg tgataatgcc ggaataatcg gcggattagc attggccaga
    25141 aaagcggtaa gaaataagca accataatta atgaatgttt cattgatact catgtcaaaa
    25201 gtatttaacc gagtaatcaa atgatcaaat gaagttggat aaagcgaaaa tgaattcaaa
    25261 taaaaaggct ctaagtcaaa tcgtgttgtt ttcattaaat cactgaaagg aaatgcttct
    25321 gcgtttcctt ttttcttttt gttgtgcgct gtataccttg aggatattca ctggtggcgt
    25381 acacttaaat agtaatatat tgtgttactt tatgtctgtg gtaatagtta tagttgctta
    25441 ccaatgtatg ttcatttttc gaaatgttga cttcataatc cttggcttaa tgtttgaatt
    25501 tttttggtga tagatttttt cagccgtata aaataaatcg taattctatt atagagagtt
    25561 gtgattataa aaagatagca agaaaaaaaa agaaagtttc tatacatttt aaatatttta
    25621 ttcctatttc tattgctaaa aaagtagaaa ttctatattt acctttaact ctcgcttctc
    25681 ttgcaaatgt agttagcatt tt
//
LOCUS       DAIGOK010000029        24271 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.029, whole genome shotgun sequence.
ACCESSION   DAIGOK010000029 DAIGOK010000000
VERSION     DAIGOK010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24271
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.029"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..572
                     /locus_tag="NOA01_000950"
     CDS             <1..572
                     /locus_tag="NOA01_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCI0632162.1"
                     /translation="ASRIAIMSEGRVIQFDTPENILRHPANDFVEELIGEDRLLQAKP
                     NATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDTLLVTDNSNVLKGFIDVETLNKK
                     RGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGLKYVPVVDEQNKVVGILTRASLV
                     DIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            576..1217
                     /locus_tag="NOA01_000951"
     CDS             576..1217
                     /locus_tag="NOA01_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI0632163.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1214..2131
                     /locus_tag="NOA01_000952"
     CDS             1214..2131
                     /locus_tag="NOA01_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HCI0632164.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2128..2799
                     /locus_tag="NOA01_000953"
     CDS             2128..2799
                     /locus_tag="NOA01_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI0632165.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3340..3828)
                     /locus_tag="NOA01_000954"
     CDS             complement(3340..3828)
                     /locus_tag="NOA01_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HCI0632166.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4029..4295)
                     /locus_tag="NOA01_000955"
     CDS             complement(4029..4295)
                     /locus_tag="NOA01_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCI0632167.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4295..4558)
                     /locus_tag="NOA01_000956"
     CDS             complement(4295..4558)
                     /locus_tag="NOA01_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCI0632168.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            5034..5219
                     /locus_tag="NOA01_000957"
     CDS             5034..5219
                     /locus_tag="NOA01_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCI0632169.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(5361..7184)
                     /locus_tag="NOA01_000958"
     CDS             complement(5361..7184)
                     /locus_tag="NOA01_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCI0632170.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7782..10487)
                     /locus_tag="NOA01_000959"
     CDS             complement(7782..10487)
                     /locus_tag="NOA01_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCI0632171.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10793..11740)
                     /locus_tag="NOA01_000960"
     CDS             complement(10793..11740)
                     /locus_tag="NOA01_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HCI0632172.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            11930..12199
                     /gene="rpsN"
                     /locus_tag="NOA01_000961"
     CDS             11930..12199
                     /gene="rpsN"
                     /locus_tag="NOA01_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCI0632173.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12375..12737)
                     /locus_tag="NOA01_000962"
     CDS             complement(12375..12737)
                     /locus_tag="NOA01_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCI0632174.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12748..13869)
                     /locus_tag="NOA01_000963"
     CDS             complement(12748..13869)
                     /locus_tag="NOA01_000963"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCI0632175.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(13885..14235)
                     /locus_tag="NOA01_000964"
     CDS             complement(13885..14235)
                     /locus_tag="NOA01_000964"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCI0632176.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(14365..15876)
                     /locus_tag="NOA01_000965"
     CDS             complement(14365..15876)
                     /locus_tag="NOA01_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCI0632177.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(16198..17550)
                     /locus_tag="NOA01_000966"
     CDS             complement(16198..17550)
                     /locus_tag="NOA01_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCI0632178.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(17703..18779)
                     /locus_tag="NOA01_000967"
     CDS             complement(17703..18779)
                     /locus_tag="NOA01_000967"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCI0632179.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            18975..20720
                     /locus_tag="NOA01_000968"
     CDS             18975..20720
                     /locus_tag="NOA01_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HCI0632180.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(21000..21437)
                     /locus_tag="NOA01_000969"
     CDS             complement(21000..21437)
                     /locus_tag="NOA01_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCI0632181.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(21421..22794)
                     /locus_tag="NOA01_000970"
     CDS             complement(21421..22794)
                     /locus_tag="NOA01_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632182.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAICLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(23116..23604)
                     /locus_tag="NOA01_000971"
     CDS             complement(23116..23604)
                     /locus_tag="NOA01_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCI0632183.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(23626..24228)
                     /locus_tag="NOA01_000972"
     CDS             complement(23626..24228)
                     /locus_tag="NOA01_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCI0632184.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         6959 a         5162 c         4489 g         7661 t
ORIGIN      
        1 tagcaagccg tatcgccatc atgagtgaag gccgtgtgat ccaatttgat acaccagaaa
       61 atattttacg tcatcctgca aacgatttcg tagaagagtt gattggagaa gatcgcctac
      121 tacaagccaa gccaaatgcg acaagagtcg gcgaagttat gctaaataca gcaatcacga
      181 ttacaccgga aaaatcatta caagaagcaa tcaaactgat gagggaaaaa cgtgtagata
      241 cgttgttagt taccgataat tcgaatgtat taaaaggatt tatcgatgta gaaacgttga
      301 ataaaaaacg aggaaaagta tccagtgtcg gcgacatttt gaataaacat gttttttacg
      361 taaaagaatc cgcctatctt cgtgatacct tgcaacgcat tttaaaacgc gggctgaaat
      421 acgtgccggt cgttgatgaa cagaacaaag tcgtaggtat cctgactcgt gcatcattag
      481 tggatatcgt ctacgatgtg atttggggag aagatcccgc aactactgat gttgcagagt
      541 cagctactcc tgaagaatct aaggaggtct gacctatgca gcagtttttt catcagcacg
      601 gttcagaaat tctctcaaaa ggttttgaac atctttatat ttcaggactt gctttgatca
      661 ttggtatcat cattgctgta ccgcttggtg tcttactgac acgttttcca aaagctgcca
      721 cgattgtgat tggcttaacc agtgccttgc agacaatccc ttctcttgct ttattggcat
      781 tgatgatccc gctatttggc gtaggtaaat tacctgcgat tatcgcactc tttatttatt
      841 cattattgcc gattttacgt aatacgtaca ttgggatgaa aaatgtcgat tggaattatc
      901 gggatgtggc aaaaggcatg gggatgactg agttccaatc cattttttcc gttgaattgc
      961 ccattgctat gccaacgatc atggctggta ttcgtttagc tgccgtttac gtgattgcat
     1021 gggctacact tgcctcttat atcggcgctg gtggtttagg cgatttgatt tttagtggac
     1081 tgaataatta ccaaccagat ctcattttgg caggtacgat ccctgtaaca attttagcac
     1141 tattggctga ttggcttcta ggtctcttgg aacaccggct gacaccgatc gcacttagag
     1201 aggagaatga agaatgaaac gtatactaaa aatgaccatg gttcttttct ctcttcttat
     1261 acttgcgagt tgttccttcc ctggtcttgc cagcaataca gatgaagaca ccatttcgat
     1321 cacaggtgga atcacttcag aagcacagat cttagcaagt ctagttgccg gaatggttga
     1381 acattataca gataaaaata ccgcaatcat caacaatctg gctactacaa ctatcaacca
     1441 tcaagcgatg atgaacggag atgcttcaat ttcagcggct cgctacacag gaacagattt
     1501 aacgactaca ttgaaccttc ctcctgaaaa agatccgaaa aaagcgtttg ctaccgtgaa
     1561 agatgaattt gagaaaagat acggtcaaac gtggttcccg tcctatggtt ttgaaaatac
     1621 gtatgtcttt cttgttcgaa aagacaccgc acaaaaatac catctatcta aagtcagcga
     1681 tctgaaaaat gtagcggatg aattagtagc tggtgtagat acttcttgga tcaatcgtaa
     1741 aggagacggt tatgacggct tccaagaaac ttatggcttc tccttcaatt cgatattacc
     1801 tatgcagatt ggacttgttt atgacgcagt cgaagcagga aaaatggata ttgttcttgg
     1861 ctactctacg gatggacgga tcgccagtta tgatctagtc atgttggaag atgatcgtca
     1921 attcttccca ccatatgatg ctgctccagt tgtagacaac aagatattag ctgatacgcc
     1981 tcatttaaaa gaagccttaa ataagttagg tcataccatc agtacggaaa aaatgcagga
     2041 attgaactac gaagcagata ataatctagt ggaaccatca gtagtagcac aacgtttctt
     2101 acaagaaaac cactattttg aagggaagtg aagcaattgg ataaaatgaa tgtctttcaa
     2161 caatttttct attatttcca ggaaaatggc tcctatatct ttgcacaatt tatccgtcac
     2221 ttcctgattt ctatctatgg tgtcctcttt gcagcagttg tcgggattcc ggttgggatc
     2281 atgatttcta ggagacgtaa attagcaaac tgggtgattc gtttggcgaa tatcatccag
     2341 acgatccctt ctctagccat gatctccatt ttaatcattg gattaggcct gggagtcaat
     2401 gtcgtgattg ttactgtatt tttgtattcc ctcttaccga tcatcaagaa tacgtatacc
     2461 gggatgatcc aagtagacaa aaacatcctt gatgtcggca aaggcatggg gatgactgct
     2521 agacaacgac tattcatggt agagttacca ctctctgttt ctgttatcat ggcaggcata
     2581 agaaacgcgt tagttgttgc catcgggata accgcgatcg gtgcattcgt cggtgctggt
     2641 ggtcttggag atatcattat ccgaggaacc aatgcaacag agggcacatc gattatttta
     2701 gcaggtgcat tgccaactgc attaatggcg atcatcacgg actggctttt aggtattttg
     2761 gaaagacggc tagatccagc cagccgtaca tcacgctgat gttattgtaa aaaacaaaag
     2821 cttctgcgac catttgttgg tcgcaaaggc ttttttagtt ctgtcatttt taaggcaata
     2881 atccactctg attcttccct ccttatgggc aaagagttgc ttttcttagc tatttatcca
     2941 ttccgtatac tccaacttca tactgccaac gggttcatat ctgcatccac cgactaagtt
     3001 tttttcttat ccgctaaggc attttgccaa tccattttcc catcactcgt catggaattc
     3061 ataccacatg atctctaaat actcgtctgc ttgttctttg aattgggcat caattcttac
     3121 atcctttggc gtggctttca tttaagcctt ttccatcagt cctcgattgt tcgatgcctt
     3181 cagctgcatc atcaaaactg gtgactgccg tatcggtaac ttcgatcatc ttttatacaa
     3241 tcgggaactt gccatcctca tccttggcta aaaacatatc tagccattcc agcttctcgc
     3301 catgggaggt ttgcggttcc taaaaccggt acacgattct tatggactgc atccgtcgca
     3361 actagacttg gcgatacgat gatccttcac cagaagagca cccccgtaaa ccaattgatc
     3421 gatatattgc caataagaaa agacattggc gtaaaagata tcgatccctc gtgatgcatt
     3481 gccgttgata ctgctattca tggccgaaag cgccacgact ttaacttctt ccgtttgagt
     3541 gacattcatg ttcggcaatg tcgctttgga tacccgctct gctaaaggaa cgacactggt
     3601 attgtatttc gtatctgtat cttttccaaa agaccatgcc aacgaatcct ctgggaacca
     3661 ataagacgac tccggttgat tgtccattgc ttttttcaac atacttttgg ctgtttctcg
     3721 ataaacgaca tttccaattg tcttcgcctc actttggcga taatcgatgg tcgttgccac
     3781 tgccaacact aacaacgatg ctccaatttt actgattttc cttgtcacac ttcttcctcc
     3841 ttgttttaca taaacccgtc aacattccat tcctaaactc aaacaatgac ccttcaaagg
     3901 tggagatata ggaacaaatt aaagaaatca tgcaataaat ccaagaaggt tggttttaga
     3961 acaaaaaaag agtctccttt ttggggagac tctttgttaa ctatctaaaa aggttggctc
     4021 ttctcacttc actcataatg tgtccaggca gaataaattt ctacaacttt attttcctca
     4081 tcaactttgt aaaccactcg atgctgtcta ttcagcctgc gtgagtatct gccttcatgt
     4141 gtaggtctca gcttttcaaa agactgcgtg ggcatatatg gatcttcctt caatgtctga
     4201 atcacttctt caaattgttt ctttaaattc gtttgcttga tttttcttaa atccactttc
     4261 gcagagtttt tgattgcgac tgtatagtta ctcatcaaag agcttcccaa tcaatatcat
     4321 cgatattcgt aaagctactg tcgtcttttt cgcggtctcg tactttatct aaagtacctg
     4381 tttgctcaag aaaaagggtt tcttgaatcg ctctccagtc ttccagccca atcaatacag
     4441 cattgtttcc actacgatca ctaataatct cgatttcagt atgattttca tttacctctt
     4501 tcaataactg ataaaaattt ttacgagcag tgctaggatt catcgttgta ttagccattg
     4561 tctctcctcc tttttctgat ataattatag cgtacttgat taagtacgtc aaaaaaatac
     4621 aataaagcac ttaaattcag caaacttttc ctctttttta catagagaaa aagacccctt
     4681 tttagggagg atctttgtcc acgctcactt cttactcctt tacgactagc ggtagccaaa
     4741 cgtaaagtaa ttggtaacaa cgtatcatct ttagcaaagg tcgaatccaa agcctgtgag
     4801 tctatgcaac aacaccaata cacgaaggtt cctcgttgca gtgtgacttg attgatatct
     4861 tgaataacct gaggatacgc acgaaagatt tgtaacggtt attcaaaact gattttggcc
     4921 gtcaagtctt ctactgcctc aaaaatgatt gcctctttcg tctcctgtca ttcatgttca
     4981 ctatcgattt gatacttgct tagaccgttg ggtttgtgaa tcaccaatta aaaatggcac
     5041 tgagttatgg taacaaaaaa tggtcacttg acgaggctga gattcctact tatgaagaca
     5101 catggcactt aagagtcgag gtaaaggaag aagctgaaaa tataggaggc tgtacattat
     5161 ttggcaaagg attcggaaat tatgatcaag acatcaaaat caaaatgttt tacagctaaa
     5221 taggaagaat aataacattt catacgctgt tcccatcttt aatggcttat agagggcacc
     5281 tttagctgag aataagacaa aacagaccct ctagcttcaa ttaagagcta gagggtctgt
     5341 ttttgatttt aaaacttggt ttatttcttc aaatggtagc tataggtgct gacaacgata
     5401 ttttcacgcc aagacaatcg caatcttaac cgcaaacttg tttggttatg gagaatattc
     5461 tgccagcgtc tgtttggtac aaattgagga atcaacacag tagttgtata gttttgtttc
     5521 gctgcatttt tacttacgat atctacataa cggataattg gattttcaat cgagcgataa
     5581 gaagagtgga cgattgaaaa acggacatct gggaaatgct ttttaaattc tgcttcgatt
     5641 tctttttctt tttcgacatc ttcgtctagc gaaacatgca tcgcaacgac ataatcaccg
     5701 atcgaacgtg cgtaattcaa cgcaccgata tttacccgtg tcacattccc gactaataca
     5761 agaacggtat tcccgtcata atcatgtagt tttgcatcat tttctaaacg aagctgttct
     5821 gctacttttt tataatgatc atggatctta taaaaaatga acataacgat tggcataatg
     5881 atgaagaaag gccaaatgtc ccctaaccga tagacaaaca aaatcgcaat gattgcatat
     5941 gagatgaacg cacccgtaat attggcaatc gatttgctta accatctttt tccttctttt
     6001 ttccatttga cgaccatccc tgtttgagat aaagcaaatg gaatgaacac gccgatagag
     6061 tataacggaa tcaagcgttc tgttgagcca tggaaaataa acagcaatac gatcgaacct
     6121 agcgctagtg tgataatccc attggaatag cctagtcgat ccccacgatc ttgatacatg
     6181 tgaggcatga atttatcttt tgccagattg tatgcaagaa ctggaaaagc agaaaaaccg
     6241 gtgttagcag caactgctaa aatcagcgct gtagcaaatt gaaggacgta atacaaaata
     6301 ccatgtccaa agacagcttt accaatttga gacaaaacag tgacttcttt ttcaggaacg
     6361 atcccatacc aatagttgat aaatgtgatc cccacgaaga agaaacctaa gatcaatgcc
     6421 atcatcgtca atgtagcagc ggcatttttt gctcttggct tcttaaagaa tggtacagca
     6481 ttactaatcg cctccacccc agttaaagag gaagaaccag aagaaaatgc tcgtaaaatc
     6541 aaagcgatag aaacacccgg cacagccgct cctggaagtg cagtagcttg caatggcaca
     6601 gcacctgtca cgatattata taaaccaaca acgatcagta acgagatcac aaggataaat
     6661 gtgtacaccg gaaataatag gaacgatgca gattccctca gcccacgaag attcagcatc
     6721 atcagtagca aaacgattgt cacagaaata gcgacttgat gaccgtagag tgctggcacc
     6781 gctgatgtga tcgcttcagc acctgctgag acagatactg ctactgtcaa catgtagtcg
     6841 atcaataaag agccaccgga aatcaagcca gcattctttc caagattctc actgctgacg
     6901 acatatgctc ccccgccatg aggataagca tggatgattt gacgatatga tagcgttaat
     6961 gaaatcaata aaataataac aaaagcggca atgggtagcg aataccagat ggctgcagca
     7021 gacaaagcga ctaaaacgac aacgatttgt tccgtaccat atgcaataga tgataacgca
     7081 tcagacgaca ataatgccaa agcagcaaat cgggttaatt tgtgctcatc attttcagca
     7141 gattttaaag gcttacctac taacaacctt tttaagtaat ccaatttcta aactccttta
     7201 cacacaattt tacttcaagt tttatagtcg aaaatttata gtaaacaaga tgttatagta
     7261 cgctcactga ccttaaaagt caataaactt taagaaaata attggaaaaa aacagaaaaa
     7321 tatcaagcgc tttttcaaac gctttaatac agcaaaaatc taacattttt ttatgggctt
     7381 tttctttgaa ttctataata aaaggataga acttgactta gaaaatcaag ttctatcctt
     7441 tttgatatct taacttttgg aagagagatg taaggctgct ttctacccat cattcttact
     7501 actcgaagca tagcgcagtt gtcacaacct cttgataaac ggtgtttaaa agcttgcatc
     7561 tgcggtaata agctcaacca aattaaaaac ataagaagct gtttttattt ggtcgttctt
     7621 attacttgat gcagagcagc ttttttacaa cctcttggta cacggtgttc aatcagctga
     7681 ccctcggtat tagctcaaaa aaacggcaaa atatgaggaa ctatttttcc atttttttct
     7741 ctaatactca ggtcaaaaca ctgatttcac aacctctttt tttaatgaaa aatatggctc
     7801 tcatgcgccg cttctttgaa tttcgtcatt ttttcttcga tgaacggata gattgccgtt
     7861 cctacgattc caaagacaac aaacaaaccg atcatgatcc agattgcttt cgtagcattt
     7921 ggccaataaa ttcctcctgc agattcccgt aacaagttta ccgcatgagt aaatggcaag
     7981 aacggattga tcatttggaa gaatttccct gagacttgga tcggatagtt cccgcctccg
     8041 ccagagatcg acagtactaa gatgataata gcgatccctt taccgacatt atcgaataat
     8101 gcagccaaga catagacgat catcatgaac gctaaggcga tcaagacagc aaatgacacg
     8161 ctgtagaccg gattttggac atctactcct agtaagaaaa tattccctaa tgtaacgatc
     8221 aagctttgcg caatcgccat tgtcaaaaat gtcagcatac gagcaacgaa ttgttctcgt
     8281 ttcgtaaaat tgtttcggtc tttctttcct aaatagtatt ctgtcgtagc aacacttgaa
     8341 agaagaacag cgccgaccca cagacatagt gcagtataaa acggggtgct agctgaaccg
     8401 ttatttgcga ttggataaag cgcattggtt tgtagctcaa caggttgtgt aaagaagtca
     8461 ctttcttttg ttgcgtctag tttcaataat ttgatgactt cgcctagatc gacttcttgt
     8521 tcgccttttc ggatcgcgtc tgctgctttt tggataccag ctttgatatt tggccagtca
     8581 ttgttgatca agtccgcagc agcgttcaca gctgtttcga cttctggcat tttttcattt
     8641 actgttgtca atgtaccggt gatttcattt tttagtgctg gccaatcatt ttggataaaa
     8701 tcagcggcca atcctaattt ttgctgaatg atcggtaatt cattgttgta taggtctgcc
     8761 ccttcattga tgccattgac gatggtatcc atatttccat tcagcattgt attggcatca
     8821 tggatttctt gtccaattgc aggcatttct ttttgatatt tttctaaaat tgccactgca
     8881 ttcgttacag ttgctttcgt tgagctgaga agcgaatcaa agtcgatctg gtccgctttg
     8941 ctcagtgcgc cttgtgcagt cccaatcgta tcgatgactt ggtctagtag agaagtgaca
     9001 gtggaactga tttgacctgt attgatacca gcaatgatgc cattgatatt attaacaacg
     9061 gcatcaattt gatctagcaa ttcatcaata gccttcgtgt cttcattttg aacagctgtg
     9121 ttcaaagcag ataggcggtt ttgcagattg cttagcaagg gttttaattg atttagctga
     9181 tcaataattg gctgtaattt cgtatttcca gcacttgttt gcaatttagt caggaactcg
     9241 accagtttat cgattgcatt ttgttgttga gatattccgt caataaagcc gttcaataca
     9301 ttgctgatgt ttttcttttc atcatcagtc agcttaccat cttcaatagc tgttcttaca
     9361 gtatctatta acgaacctgt cgttgttgcc acttgtccaa ttgacttcaa tgtaacatct
     9421 acactgtttg tgatgcttgg tatcgcatct ttcagctgag aggcacctga ttttgtttgg
     9481 tttgctagat cacttgcctg attttctaaa tttttcacat ctggcaagat atcttgtact
     9541 tgttggatga tcgtcaagcc ttgttttgct tcatcgattc cttggttcat tgtctcttgg
     9601 atcgaagcaa aatcgccgtc gatctctgct agttgcttac cggcattttg gatctctggg
     9661 attttttctt gcaaatcaag gatgattttt gcttgatctt tcaactcagg cattttgtca
     9721 ttcaatgcaa cgactttttc acccatctca tctactttag ggatgtaatc cacgaattca
     9781 tttgctttat tcagtttttc cttgatttca ggtagttgac tttgtaattc gaggacttgt
     9841 tttgtgtagc cgtcgatcgt gtccaaattt tcatctgttg ataaaatcat atctttaact
     9901 ttattgatac ttactaggtt cgtatcgatg tcgtaaccta tctcattgaa tactttcaat
     9961 aaagtcgaac tggctgtttt gataaattca tttgtgattt gcgattggat cgaacttgct
    10021 cctttatcgg tgattttcgg tgcaatcgca ttgatttttt catttaccgt atattcgatt
    10081 tttggtttct tgatatcacc actagtaaaa ctcagcagat cttctgagaa atcctttggc
    10141 aagtaaatac ctgcgtaata ctttccggaa cggactccgt cttctaattg ttcttttgaa
    10201 tcaacgaatt gccatcctaa ttgtttattt ttatgaagcg attcgatgac ttgctcccca
    10261 atggccactt ctttaccttg aaactctgct ggcttgtctg cactgtaaac agcaattggc
    10321 agctctccag tatttccata tggatcccat aatgctttga tgttaaacca cgcatatagc
    10381 gaagggatga tcatgatcgc gatgatcaga agtgttgcaa tcgggttttt gaaaatccgt
    10441 ttccagtcaa gcaaatataa tcgaagcgta tttttgattg actgcatagt tctccccctt
    10501 cttctttttt cagctgaatt actttaccat gtatttcata cttttttcaa tcggcttttt
    10561 tcaaaaaagt cgaatttttg gacatatcac ggcaattgtc tcattgtaat ctcttacata
    10621 ttgagaaata attaaggatt acagccttta ctttatcatg aaataaaagg gaaagataat
    10681 tttatgccaa agtttttatc tattatacgt atacgcaaaa aagctaaaac attttttgtc
    10741 tttgccccta caaaaatgtt tcagcttttt tcgtttttta ttcctgtatt tcttattttt
    10801 cgaatgacag gatgattttc ccagcaggag gatgtccttc aactagctga tgtgcttgga
    10861 tgacatgctt aagtgaagca gacagattca ttgaaataaa aacatgaaag gctccttttt
    10921 ggtaggcttt gattatgcca gcaaaggctt ctgcatctag atattccttt cttggaacaa
    10981 acgattcata aaagccttcc tttttttgcc gcaaatctag atctgggagg tcatttaatg
    11041 caacatacct gccgcctggt ttcatgatct ggatgccggt ttctcctttg atacttcctt
    11101 tcgtcgcatc gattacaagg tctgcctgat cagcaaattg cagacctgga tttgttttat
    11161 cataagcagc aaatgctgaa gctcctaatt tttttacctt ttcctcattt ttactggatg
    11221 cactggttaa gattcggatt cccttttcat ggagaagctg gatcagactt gacccgaccg
    11281 cacctgatgc tcctagtatc attacagtat ctgtcggctg gatttccgtc aaatggttga
    11341 ttagattata agccgtaatt cctggcgtga ccattccggc tgcttcttcc caactcattt
    11401 tatctggaat ttttgctact tttgctgatg gcaacacgac ttcttctccg taagtcccgc
    11461 cgactgcatg gacagctact cgatcaccta catgaacagt tgtgacatct gaaccaatct
    11521 ctgtcacaat accagcgcca tcattacctg gtacataagg gaatttcaat gttcgtatct
    11581 ctttcatttc tcctaaacgc aaggcaacat cataaggatt cacactgaat gcttttattt
    11641 ctacacgaac atgtccagaa ccgacttctc tgggatgagc atcgatttct tcaaaaacat
    11701 cttgtgcttc tccgtaaccg ttaattgcaa atcttcgcat ggtcaccatc tccctttttc
    11761 tttaagttta cgtttatgtc tttattttga caaaaaaaat gtcttttcgt tttggcgcat
    11821 gattcaaaac aaaatagttg tcttttttcg cgtaacgtgt taaattattt atctaaaagc
    11881 gtaaagatta cgatttacgc taataaaaaa taattggagg aatttgttga tggctaaaaa
    11941 atcaaagatt gccaaagcaa aaaaacagca agaaacgatt gaacgctatg ctgctttacg
    12001 ccaggaatta aaagcgcaaa aagattacga aggcttgcgt aaacttcctt tagatgctcg
    12061 tccagaacga ttgaaaaatc gtgatttaat cgatggacgc cctcgcggat acatgcgtaa
    12121 attcgggatg tcccgtataa aattccgtga attagcacat aaaggtttga ttccaggagt
    12181 aaaaaaagcc agctggtaat ccaagtctgg ctgtaatgta aaggaggagg gcgcatggac
    12241 cactccttgg atcatcaaaa aagatacgtt cgcttcttct agcagaaatg ctggtactag
    12301 aagcagacgt atcttttttt acgcacagct ggtccttttt tatatgtaaa ggctgcatgg
    12361 ttacttcgct atatttattt agtaacagga gtcagtgctt cgatacccac actaattttc
    12421 aatgctcggt caacttctgc cataatgggt cgatccaaat ggcagacctt ttctttcaat
    12481 cttgatttat cgatcgttct gatctgctcc aacaggataa cggagtctct ttctattcct
    12541 gttccagtag ctttgatccc aatgtgagtc ggaagcttag gtttagccat ttttgccgtt
    12601 atggccgcaa caatgatcgt cgggctaaaa tggttaccta gattattttg aataaccaaa
    12661 actgggcgtg ttccgccttg ctccgatccc acgactggag aaagatcagc aaaataaata
    12721 tctcctcttt ttaccatagg atcctcctta ttcttgatat tctcttggta tccgctgtcc
    12781 taatccgcaa gccacttcat agtggatcgt acctaattga tctgctatat cctgcatagt
    12841 gatttctttg tctgcatttt tgccgataag cgtcactttt gttccgacag gaaattcttg
    12901 aggcaatcgg atcatcagtt gatccataca tacacgccca atcgtttcac agtagttccc
    12961 ttctaccaat agagaaaaac cctgcatctt tcgtggccag ccatctgcat aaccgatcgg
    13021 tatcgttccg atccactctg cttcaggagt gatatatgtt tctccatagc cgattccttc
    13081 acctgctggg agttttttga cttgaatcaa ttcagaaacc agctcaagtg ctggctgcaa
    13141 tggataaact tccggtagtg cgtgcccaga gggattaagt ccgtacattg ctactccata
    13201 acgaatcata ttgccgattg tttcatcatg ccataaagcc gttgcactat tgctgacatg
    13261 tacatatcga ggaagttcgg gaagtttttt cagcacttct ataaagcgtt ctttttgcag
    13321 attccaatat gtatcatctg cctgatctgc tgtagaaaaa tgggtaaata aaccttccca
    13381 ttctaataca cgactttctt tgatgaatgc agctgcctct tttacttctt caggtgtaca
    13441 aaatccgata cgacccatcc cagtgtctgc ttttagatga atcgaaagag gcgtctctag
    13501 cttacttttc tctaaaacag cagctgctgc ttgcaaccat tcccgtgtac ctgcagttac
    13561 tgataaatca tgagcaacag ctacagtcag tgcgtcgtaa ggaaccatat tcaagataag
    13621 gatgggctga gtaaatccag cttctctcaa ctcgactcct tcatctaaat ttgaaacaca
    13681 aaagcccgaa gctccacctg caactgctgc ctcagctgtt tcaatagctc catgaccgta
    13741 tccgttcgct tttactactg caaacaattc ttttccttgc ggaagtcttg caacttcatt
    13801 tgctacattt tcagttatag cttttttatg tatcactgct ttcgttgggc ggtgccaagc
    13861 gacaaccatc gtttttcctt ctttctagtc ttccaaaata atttgtgcaa aagctgtttc
    13921 atttgtatga gtaatactga cccatacatt gccttcatga ggtgaacgcg tcacaacagg
    13981 ctgaccattt tcatttttta aaatctcgac atcttgaaag gtaacttttc cgatcccagt
    14041 tccccaagct ttagagaaag cctctttgca agcgtaacgc ccgcctaaaa attctacttg
    14101 gcgatgaaaa ggcaaggact ggaatagttt catttcatca gaggtcaaga ttcgtgcaat
    14161 gaattttggt ttttcttcga tcagccgagt gatcctagga agttcgactg catcgatccc
    14221 aattcctttt atcatatgct tttctctttt caataaattt agaacactct cattctacca
    14281 aattataacc attttgccac aaataagtcc acaaataaca aaaatgaaag tttctcttaa
    14341 aaaagagaaa ctttcatttt atgtttaatc gttatttgaa cggattacaa atccgcgttt
    14401 tttatcattg tgacgtttac cgccatcttt ttggctgtta ttatttttgc tataaccgcc
    14461 ttttgatttg tttccacgat aattaccgcc gtcacgacga ttacggttgt ttccgccacc
    14521 acgtttgcca tttttattgt atcctttttt ctgcattggc aatgggcgtt ctggtgtgat
    14581 cttcactggc actgcatcag ctggatcttt tgctgtcgtt tttagcaaaa gtgcaactaa
    14641 atcttgtgca gaatattctt ctaataattt atctgctgtt tgcaggtatt tatctagacc
    14701 attttctgca agttttgttt cgatttgttc aactgcagca cctaattgac ctttgaatgc
    14761 ttctttttcg gttggtggac gtaaagtcgt catccgtttt ttcgtcaagt tttcaatcac
    14821 atgcaagtag tccatttcat ttggtgtaac gaaagtcaca gacataccgc cttttccggc
    14881 acggcctgta cgtccgatac ggtgaacgta gctttctgga tcttgaggga tatcatagtt
    14941 gtacacatga gtgacacctg aaatatctaa tccacgtgca gcaacgtctg tcgctacaag
    15001 gatatctaaa tgaccgctct taaatgaacg caatacgctc attcgttttt gttgagacaa
    15061 atcgccatgg attccttctg cacggtaacc gcgtgcttct aatccgcgtg ccaattcgtc
    15121 tacacggcgt tttgtacgtc cgaagacgat tgttaattca ggtgtttgaa catcaaacaa
    15181 gcgagtcatg atatcgaatt tttcgtattc ttttgcgcgg acatagtatt gatcgatcaa
    15241 gtcagctgtc atttctttcg ctttgatctt cacatgatgt ggatttttca tgaatttaac
    15301 gccaatattc ttgatagctg gcggcatcgt tgctgagaaa agtaatgttt gtctttgatc
    15361 aggaacttgt gaaatgattt tttcgatatc ttccaagaag cccatattca acatttcatc
    15421 tgcttcatcc aaaactaatg tttgaacggt tccaagtttc aatgtgtggc ggttaatgtg
    15481 gtcaagcata cgtcctggtg tacccacaac gatatgtgga cgatctttca agccacggat
    15541 ctgacggcca atatctgctc cgccgtaaac ggcttgtaca cgtacttttt tgtcgcgccc
    15601 taaacgatat aattcttctt gtgtttggat agctaattca cgtgttggtg cgataactaa
    15661 accttgtaat tcgtgacgat ctggatcgat tttttctaac attggtaatc caaatgcagc
    15721 tgttttccct gtacctgtct gtgcttgtcc aataacgtct tttccagcta atgctagtgg
    15781 aatcgtttct gcttggatag gcgttgcttc ttcaaaacca gcgcgttcga ctgatttcaa
    15841 caattctggt gataattcta gttctttaaa tttcaaatga atcctcctat atggttgtta
    15901 actgaggatg tagctttttt gctaccccgt cgatttgttc ggtcaaagtg tattgtttct
    15961 ttttgacagt ggccacgcac gtttttatcc attcttagcc cctgtggcac caccgaaaaa
    16021 tcactttgcg aagtttaggc attagaaaag ccaaacgggt cgcgttcagc tttatcatta
    16081 ttatgacatc tggcttcaac aagttgtttc tatggcaaaa ccagtccaat ttctcttcat
    16141 ctttcggcaa taggttattc taacatagtt tttcgttttc tgcaagttta cagattttta
    16201 tttcatttgc tgaagttgtt ctaccacttc aaataagccc atgctgttgc tgccttttag
    16261 cataatacta tctgttgctg caagattttg tttgatagca tcaatcaatt tattcttttc
    16321 tttttcaaaa tagaacacag gtagttgggg gtatttttgg ttcaattctt cttttagtgc
    16381 atgcatttgt tccccataaa ggaaaatcat ggcgaatttc tcaggatcga tatgttcact
    16441 catttgcgca tgcatattta aagactcttt ccctaattcc agcatatctg ctaacacagc
    16501 gattctgcgt ccttttgttg gcagattttt aaaagtatct aataccagtc ccatggctgt
    16561 tggatttgca ttgtagacat cgctcaaaat agctgcacca tttcctgccg ttagccattc
    16621 ggttctgttt tgagtgatag aaacatgggc gagcccttgg cgtatctcag gtatggacag
    16681 tccaaagaag cgcccgatcg tatacgcaat caaagcattc gttacattat aacttccagg
    16741 cagcgggatc gtaaatgttt ctccttctac tacaaaatcg gtttgtgctt ttccttttgc
    16801 tgtcacttcc gcatgaacat cgccatttcc aataccgaat gttgtgactg tttgtactaa
    16861 tgttgccgtt aaaggtgtca gcaacggttc atctgcgggc acaacgagta atccgtctga
    16921 tacaagtccg tcagtgattt ccattttggc tttagcgatt ccttctcgcg acccaagatt
    16981 ttctatgtgt gcttcaccga tcattgtgat tgctgcagct tttggttgag ccataagtga
    17041 aagttccgtg atttcattgg cgtgatccat gcccatttcc aaaacgatct tttctgtatc
    17101 ttggggcatg tgcaaaatcg tgtaaggaag accaatatta ttgttgtaat ttccttgcgt
    17161 cttatatgtt ttgtattttt cagacaagac agacgcggtt agatctttcg tggtggtttt
    17221 accattgctt cctgtaattg caacgacttc tgcgcctact ttttccaagt aataggttgc
    17281 caattgctgc atggctttca aaggatctgc tacaggaata actggaaaat tttttggagc
    17341 ttgttcaaca ggcaagctcc aaaatgaagc agatgctccg tttgtgatcg cttgctggat
    17401 aaattcatgt ccatctcgcg taccagcaag aggaacgaat aaacttcctt cggttatctt
    17461 ccggctgtca aattcgacat tggtaatctt tgtcagtgca gagcattcgc tgcccacggc
    17521 ttgtgcaatt tcttgtattg ttagattcat tgagatctct cctaaaaaag gttgtgaaaa
    17581 aagcgtttgg cctaaacggc gggttcgcat gactctcact tccaagtaaa tttctctatt
    17641 cgtatttaga agctagaatg aacgcggtta cgcaatcact ccagcctctt ctttatttct
    17701 ctttattcat ttttttcaaa aaaagattga cgctgatggt atcgattcat tcctaactgg
    17761 atcagttctt caatcaaatc accgtatttc aagcccatat tttcccataa gagtgggtac
    17821 atactgaact ccgtaaatcc tggcatagag tttaattcat tcaggaataa ttcattttta
    17881 tttgtcaaaa agaaatcgca ccggctcaat ccgcttccac ctaacatcgt gtaagctaac
    17941 ttcgcgtact cttgcgcttt ttgataaact tcttctggca cttcggctgg aatctgcatt
    18001 tcgattttat tattgatata ttttgcttca taatcataga atgctacgtc ttttacgact
    18061 tcaccaggca aagtcgtccg aacgtcttca tttcctaata cagcaacttc gatttcgcgc
    18121 gcttcaattc cttgttcaac gattgctcga gaatcatact gataggctgt tgctaaagca
    18181 ttttgcagct cttctcggtt ttctgctttt gtaatgccga cactagaacc catattcgcc
    18241 ggtttgacaa acatcggata aagcaaagaa ccttcacatt gatcaaatac ttttttagga
    18301 ttttctttcc attgattctt aagtactggt acataaggaa cttgcggcac accagcagct
    18361 tgtaaaatat acttggtcat gattttatcc atggcacatg cactggtcaa tacgcctgcg
    18421 ccgacataag gcatattcaa tgtctctaag aagccttgga tcgttccatc ttccccgttt
    18481 ggcccatgta aaactggaaa aacgatggct ccttcttctt tgatttcgcc cggattgatc
    18541 acttttcctg taaagccttc ctctgtctgt ccacttgggt cccatgaaag atgcaagaca
    18601 tctttgctgg caggtttttc tgttagtaat ggacctttga cccattgtcc ttcttttgta
    18661 ataaatacga gttgaacttg gtaataatta taataaatgg catttaaaac tgaaaatgcg
    18721 gaaagaatgg acacttcatg ctctgcgctg cgtccgccat atagtaaagt aatcttcaaa
    18781 agattgtcct cctgtattct aaatcgttca gtgatttact ctcatatttt agcacaaaac
    18841 aaaaataaac gaataactta tttataaaaa aacgagccag cttctaaaag aacgagcgtc
    18901 atttattttt tgtcagctag attatttgtc ccgtttttta taaaaagtta tgataaagaa
    18961 aaggagggat gatgatggcg caaacaaatc cacgcagccg cttggcagaa atcattcgag
    19021 tgttcatcaa ttacaatgta gtgccaaact ttgtacagca aaaaaatccc gaacaggtaa
    19081 aaaaagcctt tgaagaacta ggccccacct tcatcaagat aggacagatg ctttctgttc
    19141 gagaagattt gctttcttct gccttcactc aaacattcaa aacattacaa gatagtgtac
    19201 ctagcgacac attttcaacg gtcaaaaaga cgatcgaaac agaactttct ctttcattat
    19261 cagatatatt cgatgatttt tcaaaatcgc cttttgcctc tgcttctatg gggcaggctc
    19321 atcgtgccaa gctgaagaac ggtgacgcgg tagtcgttaa gatccaacac cctaacatcg
    19381 ccgaagagat ccgacttgat cttcaactat ttgaacgagc gatccctttg atcaaataca
    19441 ttcctgaaac tagcgtagtt gatctaaaag gggtactcca agaagtaaaa cgttccttga
    19501 tcaatgaaat ggacttctta aaagaaagtc aaaatggcga acaattctat caaaagaaca
    19561 atggctggaa agagatccgc agccccaaga tttatgacgc tttttgttcg aaaaaagtga
    19621 tcgtgatgga agaaatgtct ggtaaaaact tgaatcattt aatgaacgcc gaaaacaaaa
    19681 ctgaaacttt tatcactgga attcaaaaca agcagctgaa acaagaagtt gcaaagcttt
    19741 tagttgagaa ctttatgaaa caggtgtttg atgatggttt tttccatgct gatcctcatc
    19801 cagggaatct gctattccat gtactaacaa aagaagaaca gacacaagct agccgaaaaa
    19861 ctgaaacagt ccatgaaaaa gagttcggtt cctttgcttt tcgagcctcc acttctgcag
    19921 aagaccctgt tgcgccatat accatcaact acatcgattt tggtatgatg gggcatcttt
    19981 ctgcaggact taggcaaaaa ctcacacaag ccgttttagc gctgtatacg aaagatgctt
    20041 atcgaatcga aaaagcggtc cttcgacttt gtcagcaaga aggctcgttt gatgaaagtc
    20101 ggttccacca agaactgaca agttttcttg aacagtatta tgattctcct attgatgaaa
    20161 tcaacttgca agaagtattc acacatgtcg tgacgatttg tcatcaaaat aatctgcagt
    20221 tcgatcgtga tatcacgctt ttgctgaaag catttgggac attagaaggg gtgatccgcg
    20281 tcctcgatcc tgaagtttcc ctcatggaag tcgcttcgcc ttttgctcaa cattattttc
    20341 tcactcatct ggatgtagaa gatacattga aacaaagcgg attagactta ttggaaggga
    20401 tgaaggctgc gcctaagatc cctcaacagc tccaccatct attagaaatg tggacttcgg
    20461 gacaaggaaa agtcaatttg gaattgaaaa agcaagacaa attattgagc aggatcgaaa
    20521 gtatgatcaa tcggcttgtt ttcggtatga ttttagctgc attgatcgta gggtcctccc
    20581 tgcttgtcca agcagcacct gtagaaaacg cagaggtcgt ttccttatta gggatattta
    20641 cgtatgctat tgccgcattc gtcattattt ttttggcaat cgatgcattg atccaaatgt
    20701 ataagaagag gaaaaagtag aaaaaggttg tgacagaaag aatcagcttc aagaaataag
    20761 aaggggtttt acataaattg tttctcaaat ttttgtgaaa attcggctta tttttcgaag
    20821 aggctacttc tgaaacaccg agaataaaaa atgtgaaaaa gctgttctgc acccagtaat
    20881 aagaacaatc aaaaagggaa catggaacac atttatcctg tttccctgtt ccctttttga
    20941 tgtattcgag cataaataaa ataccttcgt tacctactat cttctgtact tggtaaacct
    21001 tagtcaattg ccttttttaa cgcttgacga aaagacatat gatctttctc cgtaaagggt
    21061 tttggaccct ttgtccttat gccagctttt cttaactttc cactatgata acgttgttct
    21121 agaagcgtgc cgatattcgc ttcgtcaaat tcttttccag agtgatggaa cactcgcaca
    21181 cccttcggca atacaagtga agctaaacca taatcttgcg tgatcaaaaa gtcattcttc
    21241 ttaaccatac cgacgatttt ataatccgca ctatcagctc cgcggtctac gtagacgatt
    21301 tggacatgat tagcgtattc tttggtcgta taatggtcca cactggtcac gataaccacg
    21361 tcaagaccat aggattctcc taattttatg acatcttctt ttactggaga accatctcca
    21421 tcaatgaata gcctcattct ttttcgcctt cttccataaa tgataaacag ttactaatcc
    21481 gctcagtaaa agaccaacat tgatccattt tgccaagata agcagttgta cactttctat
    21541 cggcactgat tcatggttca gcgtgatcaa taatagatgg attggctctc ccatttcttt
    21601 tgctaaaaca aacaaaagcg tcatcaataa aaactggtgg atcacactcc ctttattttg
    21661 agtagtatac ttgacaacga tccggcgatc cattggaata gctgtatcag tttgataata
    21721 ttccttattt agccagctgc ctgttactac gtgccagaaa ctaagcagaa agacacctat
    21781 cgcaaaacca tttggaatca agaggaccaa taaagaagaa ctcagtccaa gcaattgcat
    21841 agccaatgga gaccatgtta aacgtttcaa aatccatggt ccgactattt ttgctgcaat
    21901 caagcctgct gcataaggca aatacataaa aatcgaaagt ttttctaatc cgtacagtat
    21961 gccaacataa aaactgctaa ataaaaaaag gaaattccct agcattccat ttagaaaagt
    22021 aaacagattt agccagcttg gcaacttcca ttccttcttc tggtgtccta aaacaaacac
    22081 gaacaagatg aacagcagca agaaaccata aatagcaaga tcaaaaagcc tttcatcgaa
    22141 taacaatcgg gcatttctca aaagaaacaa taagccaaag aatatcaaaa ataaaacaag
    22201 ctctcgcgta gctaccagat ttcttttgat gtctttgaaa tccagttcgt aacgcggata
    22261 atgactaacc gtatggtagg ccatgacgta aaaaagtgta taaatagtaa agcttgctgc
    22321 aatctgagtc ggtgcaggca actcaatcgc ttgatacagg agaaataggc agatagctgc
    22381 aaataagtac tttttcccat ccatattgat aaaaccttgc gtcttttcgt gaaaattgac
    22441 cgtggtatta gcaggtacaa gccatgctgc actcagccct agcaaaacac ttgaaaaata
    22501 aaataaagga aaataaaata cgcctaatat tccaagcaat gaaccagcac caccaatcag
    22561 cagagcgatc atcagcaacg tgtaactatc tagaccaaat cgtagactgt tgactaaaaa
    22621 tagtccagtc atccgaaagg cataaaacag cgtaaatggc aaaatcgtca gccaattatg
    22681 cgagtctgtt agtcccaaaa ataaaagata aggaataaaa ataaccgtgt tacttaagat
    22741 aaagggcgtt gtttgtgtaa aattacgccc aaatcgtttg agtttttcga acatgacaat
    22801 ggctccttaa acagtcttat aactaatctg ctcttcgatt ttagcattaa aggggaaata
    22861 aaccaatttt acagaatata ttcttgtaaa aaaaaataca gaaagaagca taagcttctt
    22921 tctgcatttt tacggtactt gattactcaa gcagacaagc ccttctccac aaaagtcttg
    22981 cccgacaccc ctgcatccgg caggtgataa agatagaaat caattatcct gtgcctccaa
    23041 aatcaattag gagctctgta aaccagagtc cttctcgacc cgtcgcataa atttacttta
    23101 acacagttcc tatgtttatg cgagagcttt tttcatttta taatcgacca tttgaaatcc
    23161 ttgaccgata tctgaaataa gttcttctgt aatctcaaat ccttcatgct tataaacctc
    23221 gattgcttga tgattcccct gattcactcg caaaaagatc tctctttcct ttgtttgcgc
    23281 agcaagttct tcataccagc gaaaaactga agaagtcaat ccttgaccac gtactttatt
    23341 tagcaaatat aatttactta aataaatttt tccatttatc tcttcataag cagtgtaacc
    23401 gacagtcttt tcttcgcttt ttaataaaaa gtaggacaca ccttctgtca gttctttttg
    23461 aatctgtttt tttgactgat aagttttcag catgtaatcc acttgttttt gaccaatgat
    23521 cggcacaaaa gcttccggcc atacttcttg tatgactgcc atcagctcat ccagttcatt
    23581 ttctgttact tttatttttt ccatacaccc acgtcctttt ttacattata aatgaccttg
    23641 cgcttgatgg aataaaaaga gcatgatcat gctatacaca cccaccgacc atggtttcaa
    23701 gaaaatcaca atctgcataa attccctaaa agataatttc gtcaaaccag ctactagaca
    23761 gatcaagtcg tctggcgcaa agggaagcag caaggtaaca actagtactt ttttaatatc
    23821 tttcgttttt cttgtcagca attcatcaaa tttctgatac ttttttggcg ataaaataag
    23881 ctggacaaag gctacgccat agtaacggac aagcaaaaat ccgattgctt ccccaatcac
    23941 taggccaata caactatata aaagcccaaa tccattgcca aacatcagca tgcccgctac
    24001 tgaggagatc cctcccggca aaataggaat gatcacttgg atcacttgca acagaacaaa
    24061 tatcatcact gcatacttgc caaattgctg gataaacgct tgcaaagacg tgctcgatcg
    24121 aaaaatccca tgatgataag cataaagaat caacacaaag aaaaaaaaca gaccaataat
    24181 gggcatgatc tgaagacatt tttgtaaaac tgatcctttt ttagacatct atattcccca
    24241 catctttttt catttattaa aactgcctcc t
//
LOCUS       DAIGOK010000030        23928 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.030, whole genome shotgun sequence.
ACCESSION   DAIGOK010000030 DAIGOK010000000
VERSION     DAIGOK010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.030"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            259..744
                     /locus_tag="NOA01_000973"
     CDS             259..744
                     /locus_tag="NOA01_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HCI0632185.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            737..1420
                     /locus_tag="NOA01_000974"
     CDS             737..1420
                     /locus_tag="NOA01_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCI0632186.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            1546..4191
                     /gene="polA"
                     /locus_tag="NOA01_000975"
     CDS             1546..4191
                     /gene="polA"
                     /locus_tag="NOA01_000975"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCI0632187.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            4236..5072
                     /gene="mutM"
                     /locus_tag="NOA01_000976"
     CDS             4236..5072
                     /gene="mutM"
                     /locus_tag="NOA01_000976"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HCI0632188.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            5036..5665
                     /locus_tag="NOA01_000977"
     CDS             5036..5665
                     /locus_tag="NOA01_000977"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCI0632189.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            5717..6049
                     /locus_tag="NOA01_000978"
     CDS             5717..6049
                     /locus_tag="NOA01_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCI0632190.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            6361..6852
                     /gene="nrdR"
                     /locus_tag="NOA01_000979"
     CDS             6361..6852
                     /gene="nrdR"
                     /locus_tag="NOA01_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HCI0632191.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            6875..8248
                     /locus_tag="NOA01_000980"
     CDS             6875..8248
                     /locus_tag="NOA01_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCI0632192.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            8248..9174
                     /gene="dnaI"
                     /locus_tag="NOA01_000981"
     CDS             8248..9174
                     /gene="dnaI"
                     /locus_tag="NOA01_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HCI0632193.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            9303..9743
                     /locus_tag="NOA01_000982"
     CDS             9303..9743
                     /locus_tag="NOA01_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCI0632194.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            9887..10588
                     /locus_tag="NOA01_000983"
     CDS             9887..10588
                     /locus_tag="NOA01_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HCI0632195.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            10585..11706
                     /locus_tag="NOA01_000984"
     CDS             10585..11706
                     /locus_tag="NOA01_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCI0632196.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            11721..12953
                     /gene="pepT"
                     /locus_tag="NOA01_000985"
     CDS             11721..12953
                     /gene="pepT"
                     /locus_tag="NOA01_000985"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCI0632197.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            13085..13993
                     /locus_tag="NOA01_000986"
     CDS             13085..13993
                     /locus_tag="NOA01_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HCI0632198.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            14028..14300
                     /locus_tag="NOA01_000987"
     CDS             14028..14300
                     /locus_tag="NOA01_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HCI0632199.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            14311..15357
                     /locus_tag="NOA01_000988"
     CDS             14311..15357
                     /locus_tag="NOA01_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HCI0632200.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            15608..18217
                     /gene="clpB"
                     /locus_tag="NOA01_000989"
     CDS             15608..18217
                     /gene="clpB"
                     /locus_tag="NOA01_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCI0632201.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(18368..19054)
                     /locus_tag="NOA01_000990"
     CDS             complement(18368..19054)
                     /locus_tag="NOA01_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632202.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(19032..19526)
                     /locus_tag="NOA01_000991"
     CDS             complement(19032..19526)
                     /locus_tag="NOA01_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HCI0632203.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(19546..20766)
                     /locus_tag="NOA01_000992"
     CDS             complement(19546..20766)
                     /locus_tag="NOA01_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCI0632204.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            20913..22748
                     /gene="lepA"
                     /locus_tag="NOA01_000993"
     CDS             20913..22748
                     /gene="lepA"
                     /locus_tag="NOA01_000993"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCI0632205.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(22842..>23928)
                     /locus_tag="NOA01_000994"
     CDS             complement(22842..>23928)
                     /locus_tag="NOA01_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCI0632206.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT         7943 a         4314 c         5079 g         6592 t
ORIGIN      
        1 aaaatcttgc caagccccct tttttgcaac ttatgataaa cggtgttcaa aagcttgcat
       61 ctgcggtaat aagcccaatc aaatcaaaaa catgagaagc tgttttttct tggctgttct
      121 tattatttga tgcaggacag ctttttcacg acctctgtaa actataggaa tctgttaaat
      181 caagggagca gaacttgcct tttacgctac ttttggtaca ataatgtatg attaaaaata
      241 aactggaggg tttctatctt gaacataggt aagccgagaa aactaggaaa aacaataaat
      301 gaaatcgagg aaggcgactc gttatcctta acagaatcta ttgaagataa ccagcttttg
      361 ctgtatttgg gattgacgaa tgatgcaaat cctttgtata tccaacatga ctttgcacaa
      421 aaaacagaat atcaacagcc aatcgttcca tcagtgatgc tgatggggat catcacaagt
      481 gcaatttcaa agcatcttcc tggaccagga tcacatgttg tcaatttttc tgtcaatttt
      541 attgaaccgg ttttccatta tgaaacactg acttttcagt ttgaggtcat aaaagtagat
      601 aagatgaagg atgtggtgac gatttctgta gaagcggaga atacgaacga agatcgtgtg
      661 ttggatgctg tcgttatggt ccaaccgcca caagttcctg aattggaaat agcaatcgaa
      721 agtgaaggtg aattgaatga ataatccaag tacatcaatg tcaggtatca atcgttttta
      781 tgcaaaagtt tatggatttt taggtatggg acttgccctt agtgcagtca cttcttactt
      841 ggctctttgg gtatttcctt tgcaattagc aacttttatc aataattttc cactaggctt
      901 tgtgggtctg tggatcgttg aactgatttt agtaatggtt ctaggcgtaa aagcacaaaa
      961 gaatccgagc ttagcaatcg gtggatttat tgcctactct cttttgaatg gcataacatt
     1021 agcggttact cttgcatact acgatattgg aacagtaacg caagctttct tgacagctac
     1081 tttgatgttc atggggatgt ctgttgtcgg agtaacaaca aaaagagatc ttagcggggt
     1141 tggaagagca ggacaggcag ctttaatcgg agtgatcatt gcactagtct tgaacatctt
     1201 cattttgaga agtagcgtag ttgagctgtt catctcccta ctgttagtcg tcatctttgc
     1261 agggattaca gcttatgata accaaagcat ccgtaatatc tatgtccaga caaacggaac
     1321 agcacatagt ggaattgctg tattcatggc attgaagctg tatttggact ttatcaactt
     1381 gttcttggcg tttctgagaa tctttggacg gaacaactaa aaaagaaaaa aattcatcag
     1441 ttggggctat accgaattat ttggtatggt cccgcttttt cttttcgcat tccgcaggat
     1501 tctttgttag aatgaataag aactttacag taaggagtta agaagatgac aaaaaataaa
     1561 ttattactcg ttgatgggaa cagtgtggct tttcgcgcat tctttgcgct tcacaattct
     1621 cttgaacgtt tcaaaaatag aaacggtctt catacaaatg caatctatgc attcaatacg
     1681 atgtttgaaa atgttatgca aaaagagcaa ccgactcatg ttctggttgc gttcgatgca
     1741 ggaaaaacga cttttcggac agaaatgtat gcggagtaca aaggtggaag atccaaaaca
     1801 cctggcgaat tcaaagaaca aatgccatat atacgtgatt tgttgacagg actaggggta
     1861 cagtattacg aattgcccaa ttatgaagca gatgatatta tcggtacttt ggcagaaaaa
     1921 gtagacaaag atcaatttga tgtcgtcgtg ctttcaggtg atcgggattt gactcaatta
     1981 gctagtaaag aagtaaaagt ggatatcact gtaaaaggtg tcagcgatat cgaaagctat
     2041 acaccagaac atgtagcgga aaaatatgat gggttgacgc caaaacagat tattgacatg
     2101 aagggactag ccggagatgc ctctgacaat atccccggtg tgactaaaat cggtgaaaaa
     2161 acagctatca agctattgaa acaatatggc tcagtcgaag gaatttatga acatatcgat
     2221 gaaatgaagc aaagcaaaat gaaagaaaat ctgataaatg acaaagagca ggctttctta
     2281 tcgaaaaaat tggcgaccat caatacttca gcgcctgtgg atgtctctat cgactcattg
     2341 aagtatgaag ggaaaaatct cgataaactc gttccgtttt ataaagaaat ggatttcaat
     2401 cagttccttt ctaaattgaa tatcgtagaa gaagaagtaa aaatggatga tattttgttt
     2461 gaagtcgttc atgaattcaa agaggaaatg tttactacgg atatggcgtt gtacgtggaa
     2521 atgatgggcg acaattatca tacagaggaa atcgtcggag tagcgtgggg gacagaaaag
     2581 aaaatttatg tcacgaacga tctatctgta ttcgaaaacc gagcgttcca ttcttggatc
     2641 actgatccga ctcggctgaa aaaagtatat gatgcgaaac gaacttatgt tgctttaaac
     2701 cgctatactg gaaaaacgaa aggaatcgat tttgatgtct tgctaggtgc ataccttctc
     2761 gatacgaatg aaaaaagcac agatatcgaa ggaatagctg ctcattacgg atacaacgat
     2821 atccaatcag atgaagctgt atacggcaaa ggggcgaaaa aaggtttacc agaagaagag
     2881 gaactctttt ttgctcattt agcgcgaaaa gtagctgcaa tcaatgcgtt gacaccaaaa
     2941 ttgagccaag aactggcaga aaaaaaccaa gaagatcttt tccggaaaat ggaactgcca
     3001 ttgtctatca ttttggcaga gatggaaata agcgggatca aagtagatgc gactcgctta
     3061 caagaaatga agggcgagtt ctctgctcgc ttaagagaga tcgaacaaaa aatctacgaa
     3121 gaagccggcg aagaattcaa tttgaattca ccaaagcaat taggagtgat cttatttgaa
     3181 aaaatgggat tacccgttat caaaaaaaca aaaactggat attccacagc cgtagatgta
     3241 ctggaacagc ttcgtgaaca agcaccaatc gtagaggata tcctcaccta tcgtcaaatt
     3301 gcaaaaattc aatctaccta tgtcgaggga ctgctgaaag tgatccaatc agatggaaaa
     3361 gtgcataccc gttatgtcca gacattgacc caaacaggac gattaagctc tgttgatcct
     3421 aatttgcaga acatcccgat tcgattagat gaagggagaa agatacgtca ggcatttgtt
     3481 cctagagaaa aagactggct gatttattct tctgactatt cacaaatcga gttacgcgta
     3541 cttgcgcata tctcaaatga tgaacatttg aaagctgcct tcctagaagg tcaagatatc
     3601 catgccagca cagcgatgcg tgtatttgga atcgaaaagg cagaggatgt cacaccaaat
     3661 atgcgtaggc aggcaaaagc agtgaatttt ggtatcgtgt atggcatctc tgattacgga
     3721 ctttcacaaa atctaggaat cagccgaaaa gcagcacagc aatacatcga tacgtacttt
     3781 gaaaaatacc caggtgtcaa agaatatatg gaaagaatcg tacgcgaagc aaaagaccaa
     3841 ggctacgtcg aaacgctgta tcaccgccgt cgatatctgc cagacatcaa ttcaagaaat
     3901 tacaatatcc gttcttttgc ggaacgaaca gctatcaata caccaatcca aggaagtgct
     3961 gctgatattt tgaaaatcgc gatgatcgaa ttggataaac gattaaaaga aacaggtctt
     4021 caagctacga tgctgttaca agttcatgat gaacttgtct tcgaggtacc ggaaaaagaa
     4081 cttgaatcat tagataaatt ggtgaaggaa gttatggaac aagctgtttc tcttcatgta
     4141 ccactgatca ctgacagcag ttggggaaaa acgtggtacg aagcaaaata aacattgttt
     4201 aagacagagc aatataatga aggtgaaaag aaaaaatgcc agaattacca gaagtagaaa
     4261 ccgttcgtaa aggattggaa cggttagtag taggaaaaac gattcaaaaa gttcaggtct
     4321 tgtggcccaa gatcatcgag caaccagaga cacccatctt tgaggcttct cttgttggcg
     4381 aaaccatcca atccattgga cgtagaggga agtttttaat ttttcatctc gatcattgtg
     4441 aactgatctc acacttgcga atggaaggaa aatatcaatt tacgaaagaa aatacaccaa
     4501 tcgataaaca tacgcatgta cttttctttt ttgaggatgg aagccaactt cgctacaacg
     4561 atgtacgaaa atttggtcgt atgacaatcg tcgaaaaagg agcaagtgca acttaccgag
     4621 gaatcatgaa attgggacct gagcctttgc cagatagctt cttattagca gatttcgcaa
     4681 atggactgaa aaagtcccac aaagcaatca agccattgct gctagaccaa cgtctagtga
     4741 ctggtctagg taatatttat gtagatgaag cattatggga agcgaagatc catccagaac
     4801 aaccggcgaa cacactgcgc cccaaagagg tagaacaatt acgtctatcg atcattgatg
     4861 ttttggatcg tgcaatagaa gccggaggta ccacgattcg cagttatttg aatgcattag
     4921 gagaatcagg cggatttcaa gtagcactgc atgtctatca gcaaactggc aaaccatgta
     4981 tacgttgtgg aacgccgatc gtgaaaacaa aagtggcaca aagggggacg cactattgtc
     5041 cgaattgtca aaggctaaaa atcgcaaagt aggatttgtt ttaggtttga cgggtggaat
     5101 agctaccggt aaaagtacgg cagccaaagt ctttcagtct catggatttc ctttgatcga
     5161 tggcgatgtg attgccagag aaactgttga gcctggaacg cttgctctga ggaaaattgt
     5221 atcagtgttt ggccaagagg tattgcagcc tgatgggcaa ctagatcgag ggaaacttgg
     5281 gatgattgtt tttccatcaa aggaaaaacg gcaaaaactt gatcagctat tagatccgtt
     5341 tatccgaaaa gcgataaaag atcaaatcgc ctctctaagt tccaatcatc ctttagtaat
     5401 tgtagatatc ccattgctct acgaaggtca ttatgaccac tatatggatg cagtagctgt
     5461 cgtttatact acgccagaaa ctcagcttca gcgtttgatg aaacgtaatc aactgacgct
     5521 agaacaagcg cagcagagaa ttagtagtca acttccaatc gaagaaaaaa agcagcgtgc
     5581 ggatattttg ttcgacaaca atggaacaaa agaaaaactt gtgggacaaa tcgaaaattg
     5641 gctttttgct aatcgctttc tttgagtatc tgtgtcaatt ttgctatcct gagtatatag
     5701 aaagtgaggg atagcgatgg gatcattaaa aaatcaaaag gataaagtcg taggtaacgc
     5761 aaaagaaaaa atcggcgaga tgctcaataa agaaaatcta atcgatgaag gtcgtacgca
     5821 atcagaaaca gcagaagaaa aagaaaagca gtaccaagca agaatcgatg aaatagaaga
     5881 ccagcaggca gaagaagaag cgaagcggca agatttgact caagaacgaa aaaatccgac
     5941 acctgatcgt agtaaaacac cgctcccaga aagtgaccac attcccgatg agcaaaaaat
     6001 cgaggaacag cgaatgaaac actatacagg aatcgaagag aagttataaa atagtaaaaa
     6061 atcgagatgt gacttgaagc agatgagaag gtgttcatct gcttcaagtt ttttgtgcta
     6121 gatgactaaa tcagcttttg tgtaagttat ttctcagtaa tcaaggtgat cagtattgga
     6181 aaattgaaat ttagtaagga caggggacac aattacgcat gaaagtagaa aaaattgttc
     6241 ttatatccgt gttttttggg taggacagtc gcgtttgtga tttgtttcgt gttataatac
     6301 aactagttgt aaagaaagac acatccttta tataaaatgc gatgcaagag gtgagacaaa
     6361 atgagatgtc ccagatgtca acataataat tcacgagtaa tcgacagccg tcaagcagat
     6421 gatggacgtg cgatccgtcg ccgtcgcgaa tgtgagaatt gttcgttccg attcactaca
     6481 tttgaacgaa ttgaagctgc tccgttatta gtaatcaaga aaaacgggga acgcgaagag
     6541 ttcaatcgcg ataaaatttt aagaggactc atccgttcag cagaaaaaag acctgtagct
     6601 atggaacaaa tggaacaaat cgtcaaccat gtcgaaaacc gcgtacgcag tctcggagaa
     6661 aacgaagtat cgacgaatct aattggtgaa tatgtgatgg aagatttagt tgatttagac
     6721 gaaatcgctt atattcgttt tgcaagtgtt tatcgccagt tcaaagatat gtctgttttc
     6781 ttaaaagagt tgcaagatat cgtggataaa gcaaaagaaa ataataaaga gaataataaa
     6841 gaaaacaatt aagaaacaaa aaggaggcat ttccttggaa aatgcttgga aagagcttca
     6901 accaaaaaat atttaccaag gaagaaaagc ggctccgctg agagaagaca gcaagaacgc
     6961 cttgctgtat ctttatcagc cactaatcgg cggagaagcc ttgagtatct atttcaccct
     7021 gttatcagaa atctctcaag aatctggcaa aggtcctgaa gggatgcatg cagatctgct
     7081 tagtagtcta ggctgcggta tcccgcaatt ttatgatgca agaaaaaagc tagaaggaat
     7141 cggcttactg gatgtttatt ttaaagaaga tagtgatctg ggacctcgtt ttatttatga
     7201 gctgttagaa ccactcattc ctaaagcatt tttcaaagac tctgttctgg cttttcttct
     7261 tctggaaaaa gttggtgaac gacgattcca gaagcttacc caattctttg aacctaaaag
     7321 gcatacagca gagggatatc aaaaaatcac aaaaaaattt ttagatgttt atcgcttcaa
     7381 tgaagcagtg tacgctgcta atcaagaaaa catcgaatcg ctgaataaat cttttgaaga
     7441 atctgaatca gtagcattac ttgaagcaga ttcgacattc gattggcagt ttttgaatga
     7501 ttggctggca aaaaaacata ttgcaagtct tgatgatcca atcatcaaac aagtcgacat
     7561 gtatcatcag ctttacggtt tcgatgaatt gacacttggc gaactgatca gtcaatctta
     7621 tgactttagt gaacaaaaag tatctgaaaa agaactgcaa aaagccattt tggcaatgca
     7681 gagacgtccg gtgaagcaaa caaaagaaac agttgcggtt tctgaaaaca gtaatgaacc
     7741 ctcgtcaaac gaacatttga caaacgcagc gcttcagctg ataaaagaag cacagagcat
     7801 tccaccgatg aaatatctgg aagcaatcaa gtcagaaaaa ggcggatatg tttctaaaca
     7861 agaaaactgg ttactgcaag atcttgtttc tcaatcaggt ctttccagta gcgtgatcaa
     7921 tgttttgctg aattatgtac tagtgatcaa gaataatgct agcttaaatg ccagttatgt
     7981 aaatacagtg gcaaatgaat gggcacaaaa gaaaatcgcc acagcagaag aagcgatcca
     8041 gcacattcgg caaataagta atcaagcaaa acaatcaaaa caaaagaagc aaacaaatta
     8101 tcaaactggc aagcgaaatg ttcgacggga aaagcttcct gattgggtca accagccaaa
     8161 agatgaaaaa cagatcagca gcgagaaaaa agcagagatt gaccgccgtt ttaaagaata
     8221 cctggcaaaa aaggagggtg atatctagtg gaagacgtag gcaaagaatt gaagaggatc
     8281 atcagcaaac gcaattatca agaaaagtat gctgaaatga tcgctgaagt cttaaatgat
     8341 gcggatgttc aagcttttct aagcgcacac caagaagaat tgacacaagc ggatattgaa
     8401 aaaagttacg ctaaattgta tgaattcgtg caagaaaagc gtaaatatca aaaaaacgat
     8461 ccaacgatga tcgctcccgg ctatgagccg aagctgacgt tgaattttca ttatgtggat
     8521 gtcacttatg taccaacgga agcattgtta gcacggcaaa gacaagaaga aatcagaagc
     8581 cgagtaaaag caatggatat gccaaaagat attcaatcgg cttcttttga aaactttgaa
     8641 cgtacagatg gacgaggata tgcgagctta gaagccctag attttgtaga gaaatatact
     8701 gctgatccaa aagggtatca taaaggactg tatctagtag gaagttttgg tattggaaaa
     8761 acatatttat taggagcaac agcgagagaa ttagcgattg ctggattcac cacgacgcta
     8821 gtccattttc cgacatttgc cgttgagatg aaacaggcaa tcggaaaaga tcaagtggca
     8881 gaaaagttag atgcagtgaa acgttcacct attttgatga tcgatgacat tggtgcagat
     8941 gcgatgagta gttggatacg agacgaagta ttcggtgtta tccttcaata tcggatgcag
     9001 gaacagttac caaccttctt ctcatcgaat tttacaatgg atgaattgga acagcatttg
     9061 tccgtcactc aacgtggaga tgaagagccg ctaaaagcga aacggataat ggaacgcata
     9121 cgttatttga caaaagaaat cgaaatgact ggtcgcaacc gacgcaatgg ttgaacagtt
     9181 gacaagtcca actgacattt gacctagtag taaaagcagg tcagcgaatc attatatgga
     9241 cagaaatgac cggagacaat caccgcaaca agtaatgcag aaacttaaac ggaggatttt
     9301 ttatgagggc tattttttct agtatatctg tagttggtct attttttctt ttagcaggct
     9361 gcggtagcgc agatagcaac acaggttctg ttgattcgat tgatgcgggt caaacagtaa
     9421 aaacaagcag tacgaaaacg gagagacaaa gcatatttgt cggaacgatt gaaagtatca
     9481 ctcaagtaga agcagaagaa gaaacgcttc agttgaaact cgtcgatgtc atggccaaag
     9541 aagatccagc gaatatggga acgtcattca gcaatgacgg agtgatctta aatgctactg
     9601 ctgaaaaaat aagcgtggac ataaatgaat tgaaaatcgg tgacaaagtc caatttacat
     9661 tagttgagat gccgatcatg acgatgtcta tcccaccgca agttcctgga gattcgatta
     9721 ttgatgtttc attgaacaaa taaccaataa cattttttca aaaataagaa aatgttgttt
     9781 tatagaacga aaaggtatat tttttaagga gatatgggaa tttttttatt cctagtctcc
     9841 ttattttatt tggaaaatgc tataattatc tagaaggagt gaagctatga accatttaga
     9901 tttatctcag cgcttagccg cagtgggtga ctttgtccct gaatccgctc gtttagcaga
     9961 cattggatca gatcacgctt acttacccgt tgcattgata ttaagaggaa aaatcgaata
    10021 tgcagttgct ggtgaagtcg ttcaaggtcc ttatgaatca gcacgtaagc aagtcaaaaa
    10081 agatggatta gaagaccgta tcaccgtacg ccttgcaaat ggtctagatg ctatccagcc
    10141 acaagatggt attacagctg tcacgattgc gggtatggga ggttcactga tccgagatat
    10201 cctagaagct ggcaagttga atcaacgctt aagcggacaa gaacgtttga tccttcagcc
    10261 aaacatcgga gagcgtacat tacgatcttg gctgcaagaa aatcagtata aaatccaatc
    10321 agaaaaaata cttgaagaaa ataaaaaaat atatgaaatc attgtagccg aaaaagaaca
    10381 aaaaccagtt acttacaatg aaatggagct gatgttcggg ccatttctgc ttgaagagaa
    10441 aagtgagatc ttcaaatcaa aatggacaag agagcttcaa caaagaaaag ctatccgtga
    10501 acagctcaaa caagcagcta atcaagaggc acgtcaaaaa caaatcgagc aggaaatcca
    10561 aatgattgag gaggttttaa agccatgaca atcagcggac gcactttcat ccaaaagttt
    10621 gaaagctatt gtcctcagtg gctagctgaa gaaggagacc cagtcggact gcatatcgga
    10681 acattggata agccaatcca acgagtaatg atgacattag acgttcgtcc agaagtggtc
    10741 gaagaagcaa tcaagaaaaa aatcgatttg ctgattgcta aacatcctcc aatctttcgt
    10801 cctgtaaaac ggctggttac tgatcagcca caagaaaaaa tgtatgcaga tttgttgaaa
    10861 catgatattg ccgtctatgc agcacacacg aacatggata ttatttggga tggactgaat
    10921 gactggtttt gtgaacttct cggtattgaa gtggagagtt atttagtaaa gactcatgag
    10981 attcattata aaaaactggc cgtttatgta cctgtggatc atgcacagaa aatgcgtgaa
    11041 gtattagctg cagctggtgc tggtacacaa ggtgattata ccggtacaag ttttacctcg
    11101 attggacacg gtcgatttac tccggaagct ggtgcacaac cagcgattgg gaaagtaggg
    11161 aagacagaac aagttcaaga ggcaaaagtt gaggttattt tgccggaaac tatcgaaaaa
    11221 caagtgattc aagccatgcg gtcagctcat ccttatgagg aacctgctta tgatcttttc
    11281 gctatagatg aaccagtcga aatgtttggg ttaggacgag taggtgaatt gccgcaagaa
    11341 atttctatag aggcatttgt agaacaagta aaagaagcgt tccaactaga tggactgaga
    11401 atagtccagc caaaaaatgc aaaatcctct gtgaagcgca ttgcgatttg cggtggaagc
    11461 ggtgaaaaat tttatccgca agcaatcgcc caaagagcag atgtctatat cacgggagat
    11521 atctattatc atacggctca tgatatgcaa agcgctgggt tgattgcaat tgatcccggt
    11581 cattatattg aatcgttatg caagcaacga tttatcgaga aatttgaatc ttggaaacaa
    11641 gaagaaaatt gggatattaa ctttttcgtt tctgaaacaa atacaaaccc atttcaattt
    11701 aattaaaagg agagaatact atgtacgaaa atcttttgcc acgtttttta cgatatgtca
    11761 aaacagaaac acgctccgat gcaacaagca aaacgactcc atcgactcag acacaagttg
    11821 cttttgcgca agaattgaaa aaagaattag aagagttagg cttagctgat gttttctata
    11881 atgaagataa cggattcgtt atgggaacat taccaagcaa tgttgatcat gaagtgcgtt
    11941 ccattggatt tatttcccac atggatacag cagattttaa tgcagtagga gtcaatccac
    12001 aaatcattga aaactatgat ggcgagtcta cgatcgtact agatgcggaa gaacgctata
    12061 cattgaatac aaaagatttt ccaaatttga aaaattatga aggccacaca ttgatcacaa
    12121 cagatggcag tacattactg ggagcagatg acaaatcagg gattgcggaa atcatgactg
    12181 caatggagat cttgatcaat aatccttcta tccctcacgg taccatcaaa gtggcttttg
    12241 gtcctgatga agaaatcggt gtcggagcgg ataaattcga tgttgagcaa ttcgatgtag
    12301 attttgctta tacagtcgat ggtggtcctt taggtgagtt gcagtatgaa acgtttagtg
    12361 ctgctcaagc aaatattacg atccaaggga aaaatgtaca tccagggacg gcaaaagata
    12421 caatgatcaa tgctctacag ttagcaatcg attttcataa tcagcttcca gccgatgaag
    12481 ttccagaaaa aacggaagga tacgaaggtt tcttccattt gatggcattg gatgggaatc
    12541 ctgaagaagc ttctatgagt tatatcattc gcgatcatga tcgtgaaaaa ttcgaagcac
    12601 gcaaagcaca aatcacacat atccaagaag agttgaatca gcgatttgat caagaacgaa
    12661 tcaaagtaga tatgtatgat caatattaca acatgaaaga aatcattgaa aaagatatga
    12721 gcatcgtgga attagcgaaa caagcaatga tcgagcttgg tatctctcca gtgatcgaac
    12781 cagttcgtgg aggaacagat ggttcaaaaa tctcttacat gggtattccg acgccaaatc
    12841 tatttgccgg aggagaaaat atgcacggac gattcgaatt tgtttcttta caagtaatgg
    12901 aaaaagcaac agacgtgatt gtaaaaattg cagaattgaa cacaaagagt taaaatcgaa
    12961 aaagaacagc ttatgacaag gcgttcgtcc caatgatgga atgtcatctg tctaaggcta
    13021 aagggcagga caatggaatt ggttgtcccg ccctcttttt tgaagagtaa ataggaggta
    13081 aagaatggaa aagctgctag ttttttataa caaaagttca gggaaagatg agggtgagaa
    13141 attagcacgc tggtttcaag attatgtaaa agaaaagcag ccagaattat atgtagaact
    13201 agctgaaact ggcccatcga ttgatgatgc ggaattagtc aagaaagcag aagccataga
    13261 agctgatact ttagtaatta taggtggaga cggcacgatt catcatatcg tccaagcgtt
    13321 ccaaaaaaca ttatctcaat accatgtcgg attacttcca ggtggaactg tcaacaacct
    13381 tgcacgtgtt cttgagattc ccctcaaaaa agaagaagct gctgacgtga tcctggaagg
    13441 caatgtacgg aaaatagact atggtcaagt caatgatgaa gtcattatct ctacactgac
    13501 aatcggaatc ttagcagata cagcagtttg gatcagtcaa gaagaaaaac agcgttacgg
    13561 ctcgtggatc tttatcaaga agttttttaa gttattgctg aagaagaaaa aatatcattt
    13621 ggatttagaa acagacgagg agcattggaa aggaaaatcc caactgttga cagtcacgat
    13681 gtctaactcc gtgggcggtt ttacaaattt tgacgactca gcgaccctag atgatggaaa
    13741 atttcatgta acgatcgtac cttccttaga tattttccga tttatttttt atttgcctag
    13801 aatcatgaga ggcaaattct atacgatccc cggcattact tatttcacag ccaaacgcat
    13861 gaaaatccaa gcgcaggaaa aatctgtggg cactcgaacg gatggagaca cgacagatga
    13921 tcttccagtt gaattatctg ttgtatcaaa aggattaaca gtttattcac caaagaaaga
    13981 agaaaaagct tagaaatagt agttaacaga aagttggaag aagagtaatg gatgtattgt
    14041 tacacaatcc tttgtggttt taccttttca ttgtgaattt ttacttattt tgtctgatgg
    14101 ggtatgataa acatcaggct agaactagcg gctggcgtgt tcctgagaat aatttgatat
    14161 ttgtcagcct gttaggcggt ggtttgggag gattgatctc tcgaaaagtt ttccatcata
    14221 aaactcggaa aaagaagttt actataggat tcgctgcagg tgtacttgtt gatttgattc
    14281 tcatttattt tttccattaa ggagcaggag atgtcgtcta aaggaacgaa aactaaaatc
    14341 attctgaatc tgcttttgct gattgtgatt tttgccatta tttattattt aatcaatcaa
    14401 tcatttgcag atatctttgc cgaattgctg tctaccagtg ttgttgtttt ggcagcggct
    14461 gttatattag ggacagttta ccaaatcgct gaagggcgct caatcaaaga aattgctgaa
    14521 cctttcgcgc cgaattttac aacgaaagat gggttcttga cctctagcta tattgcgttt
    14581 taccgaatcg tttcgtttgg tacaggaacg ctgatctcgg agatttattt ttataacaaa
    14641 aaaggaatga agctatccca gggagcaggg gtgacagcac tccatatgat tatgtacaaa
    14701 ctggcagtga tcacttatgc attagctggc ttggtcatcc aattctctct tttttattct
    14761 aatggtccat caatgatctg gtttatcatc gctggtatcc ttttgacgat cgtgattgtt
    14821 gcctttttat tggcgatgtc cgtcagcttg aatgttcaaa tattttttgt cagcttatcg
    14881 aataagttat ttaaaagtga gcgtttgcgg aaatgggtgg atacatgcaa tcagcagatc
    14941 tactctttaa gggaaacggt ccaaaccatt atccaagaca ggactgcgct attgcggatt
    15001 tatctatgga atatgttaaa acttgtattt tggtatgtac ttccttattt gattctgatt
    15061 gaaaaccatc ctaaaataga ttttctactg accatttcgt ttatcagctt tgcagtagtg
    15121 ctttcagggg ttattcctac gcctgctggt attggttcct ttgaatttgt ctatttgtta
    15181 ttattcagac cagttgttgg aacagttgac gcagtttctt cgcttttact gtatcgtttc
    15241 agttccttta tcttgccatt tctctatgga tttgtgtatg tgattgctga acggcgaaga
    15301 gtgatcaaag aagaaataat agaagtaaaa aaagagaata aagaaaatca agggtaggtc
    15361 ttttataaga tctacccttt ttatcaaatc aaagaaaaac gttatggata agataatttt
    15421 acgtttgttt ggctcttttt ttatatacat acaatcaatt ttgttttttt aagatttata
    15481 taagtcgaga aaaaaagtca aaaaactatg tgtatatttt gaccaaaatt gaccaaagcg
    15541 atataatagt ttttgtaaag atcaaagaaa taagtgatct tacctttaat taggagggta
    15601 tacctgtatg aatattgaga aaatgactac gacgcttcaa gaagcgattg ctgaagctca
    15661 acagattgcg gtcactcgaa aacatcaaga tatcgacatt gcccatgtat ggaaaatctt
    15721 tttacaatct aatcattttg gtcgaaattt ctatacggat gccggattgg atgtcgaatc
    15781 ttttgaacat gaaattgatc gtctactgga tgagtatcca gttgtttctg gtggaaacgt
    15841 ccaatatgga caaaacctca gccagaattt ctttcgatta ttgaatgaag cagaccaaat
    15901 acgtgaaagc ttcggcgacg agtttctttc aacggaagtc gttattttag cgttgatgaa
    15961 attaaaaaat tatccgttaa ctgtttattt gaataaaaac ggattatctg aaaaagaatt
    16021 gcgtaaaaac atcgaagaga tgagaggagg agaaagagtg acctcacaaa accaagaaga
    16081 acaatacaaa gcattagaaa aatacggcgt tgatttagta cagcaagtca aaagcggaaa
    16141 aatggatcca atcatcggtc gtgatgaaga aatccgtgat gtcatccgta ttctttcacg
    16201 aaaaacaaaa aataatcctg ttctgatagg agaacccggt gtagggaaaa cggcaatcgt
    16261 tgaagggctt gctcaacgta tcgtacgcaa agatgtgcct gaaaatttaa aagataaaac
    16321 gattttttca ttagatatgg gtgcattgat cgctggagca aaattccgtg gtgaatttga
    16381 agaaagacta aaagcagtct tgaaagaagt caaaaaaagt gatggacgta tcctcttgtt
    16441 catcgatgaa atccacaata tcgtaggggc aggaaaaaca gaaggaagta tggatgccgg
    16501 aaatctgttg aaaccaatgc ttgctcgcgg tgaattacat ttgatcggtg ccacaacttt
    16561 agatgagtac cgtcaatata tggaaaaaga caaagcgttg gaacgtcgct ttcaaaaggt
    16621 attagtcaaa gaaccaacag tggaagatac gatcagtatt ctgcgtggat taaaagaacg
    16681 ttttgagatc caccatggtg ttaacatcca tgacaacgca ttagtagcag cagctacttt
    16741 atctgaccgt tatattactg atcggttctt accggacaaa gcaatcgatt tgatcgatga
    16801 agccagtgca acgattcggg tagaaatgaa ctctatgcca acggaattag atcaagttac
    16861 tagacgtttg atgcagttgg aaatcgaaga agctgcactg aaaaaagaat cagatgatgc
    16921 aagtaaaaaa cgcttgaaaa atttgcaaga agaattagct gaacttcgtg aagaagcaaa
    16981 tgcgatgaag atgcagtggg aaacagaaaa agaagaagtc aattctgttt cagcaaaacg
    17041 tgctgaaatc gataaagcaa aacacgaatt agaagatgca gaaaataact atgatttaga
    17101 acgcgctgct gtattgcgtc atggtacgat acctcaatta gaaaaagaat tgaaggaatt
    17161 agaagctaaa gcaaaagaca gcgagattaa gatggttcaa gagtccgtta ccgaaaatga
    17221 gattgctcaa gtcgttggca gattaacggg tatccccgta acgaaacttg tagaaggtga
    17281 acgagaaaaa ctaattaaat tgaatgaaac acttcataaa cgagtgattg gtcaagatga
    17341 agcagttgat gcggtcagtg acgcggtaat tcgttcacgg gctggattgc aagacccaaa
    17401 tcgtcctcta ggttctttcc ttttcctagg accaactggt gtcggaaaaa ccgaattagc
    17461 gaaagcgtta gctgagaact tgtttgactc agaagatcat atggtacgga tcgatatgag
    17521 tgaatatatg gaaaaacatg ctgtgtctcg tctagtcgga gctcctccag gctatgtagg
    17581 ttacgaagaa ggtggtcaat taacggaagc tgtcagacga aatccttata caatcgtctt
    17641 gttggatgaa atcgaaaaag cgcatcccga tgtcttcaat atcttgttac aagtcctaga
    17701 tgacggacgc ttgactgatt caaaagggcg agtagtagat ttcaaaaata cggttctgat
    17761 catgaccagc aatatcggtt cacagttact gctggaaggc gtgactgctg atggaacgat
    17821 tccagaagca gtggctgaac aagtcaacac gctattacgt ggaaacttca aaccagaatt
    17881 cttgaaccga atcgacgaca cgattttatt tacaccattg agcctagaca acgtgaaagg
    17941 aatcgttgac aaaatggtgg cgcaattggc acaacgtctg gaacatcaag aaatcctgct
    18001 gacaatcagt gatgaagcca aaacgtggat tgctgaaaat gcttatgaac cagcatatgg
    18061 tgcaagacca ttgaaacgat ttatcactaa ggaagtagaa acaccactag ctaaagaaat
    18121 cgttgcagga catgttatgc ctaaatcaaa agtaacgatt actttacttg atggacaact
    18181 tcatttcaaa acagaagagc tagaagaaat cgtctaatct tccattgaga ctataaaaga
    18241 aaaaaggggt ctgacacaac tagtgtcaga cctccttttt tcaaataaac agtgttccag
    18301 aagtagtttc ttcgaaaaat aagccgaaat ttcttgaagc tgcccgcttc tgtcacaacc
    18361 tcttttatta ggtatttttc actttttcta atttataact ttcaaagtaa cgctcatctt
    18421 ttttactttg agaccatgca aacaatccca ttaaggcagc acctatcgta tttgtcagca
    18481 agtcgcccat cgtatccatc aatgctgctc gtccgacaaa cagtgtgcca tcagaagcag
    18541 caaaacgttg aagattcatg cctaagaact ggtcgcataa gaattcccag aactcccaaa
    18601 acacgccgca aagtccagca aacgcaaagc cgaataagag gaacaaccaa ggactggttt
    18661 ttgatatctc agcatctttc atcaagtaac caatcaatcc atagccaaga gctgtcagga
    18721 ccattggact caccgcatgt aagattttgt cccaaaaaga gatgatactg atcaaatgca
    18781 tccctgtgcc taaaaagaca gagatgaaca agaaaaacca gtaaaacaaa acgattgctg
    18841 ctggtatttt aagtttgaaa actttcaaaa tgacttgtgg catgaaaatc aatgcaatcc
    18901 ctaataaaga ttccacgata aatgtgattc cttgagaagt cgaatatttt tgtgtgataa
    18961 attcataaaa attatagcag acaacgaata caccaaaagc aagtaaagct atttgcacct
    19021 ttttgatttc tttaatcact gaaagtccct ccatctaaaa atctttttac ttctgaaagc
    19081 aactcaattt ctgaagaaac cacttttccg ttcaatttga tcaaacctat tgtatataag
    19141 ttcaggtagt gaaattgatt ttcagctgtt tcttgtagtg cagcgatttt tttcgggtgg
    19201 gttccgcctt gctgtcgagt atcagtatat aagccgatca ctgggatgcc tttcgcataa
    19261 gccacaccga tttccgatgc taccccggca tcgatcgtca gcccatctag taaagcaatc
    19321 attaaatcac tagccaacac tttttctgtg tcagcaagag caatcattcg actgtctgca
    19381 taagcagttt tgtcattgat tgcttcattt tcttgaggaa gatagatcga aagctcagaa
    19441 gagagctctc tgatttcttt aactaactgt tggttatata acaaatcact tttagcaaac
    19501 attggtgcgg caaaataaat tttcatctaa acatcatcct ttttatcaaa aataatcgct
    19561 aaagtaagct tccatgtttg gaatgatacg ctctagtgac ttcagtgttt cttcatctgt
    19621 ttctaaccgt tcgattcttg ccaaataaga agcacggcga taattcatca tcaagcaagt
    19681 caatgtttgg atatccagtc gaacggattt tcctataggt tcatttaaga cacggacttg
    19741 tccctgttca tcccatgtta aagcgaagac accattattc caaggagcga caggatcttc
    19801 aatgatgaaa tgaaaagcat cagctgttcc aacaaacgga aaccgttgaa ggaattcttt
    19861 tacatcaacg atccgagcca taaaatatgg ttcgatctgc tctttgatct gactatcttc
    19921 aatcaaaaat gctaaaggct catttttata aatatctcca tgtacccagt agatcatgga
    19981 aaaatgtgcg gaaataaagt tccataagcc atttcgagct tcttgatcaa gatagaacat
    20041 ttcttttacg tgaaatattt cttcagctac ccagtagaac agaacacctc ttggttcatg
    20101 gttgctatcg tagtagacag cggctgtgcg ctcatcttca ttttcgaaac gccaatactc
    20161 ttcccaatga aaatcactgc ggaataaagc accatggttt tgttgggcaa atttgtcata
    20221 aacggaaaat acgtctggat gatcaactgg taatcgctca accataccac ttactgggat
    20281 ttgtttaggt agctgtgtat ctctgatttt aaaggaaagt ttatcagaca tgatttccca
    20341 tcctttacgc cgatagtagg ggatactgta aggatacaaa taagagatcc attgcttatt
    20401 cttgcgcata ttatccaatg caacgataat tagatcttgc atcaatccat ggttagcata
    20461 ttctggataa gttccaactc cagtcacacc acccatttta taacgtgcac cgtgaatatt
    20521 gacttcacaa ggatagatag cgatttgaga gatcagttta ttttcgtgga accatccgaa
    20581 cactttcgat acttccaaaa tcggtttttt ggaacggata aaagctcgtt tattctcaaa
    20641 cccgctttcc tcgatgtctg cttcagtcac ttggaacaca taggaaagca attcattgaa
    20701 ttgatcgata tgttcttgag tgaccggttt taattcaaga ttttctctaa aatctttttg
    20761 atccattttt tcatctccat ttattagctt gataacagta tagcaaaatt ctacaaaaaa
    20821 aacttctata gctgactaat taaaaactct ttaaattact gcttttcgtt ggtataatgg
    20881 aaagtgctga ttatatgaaa agaggagacg ctatggatat aaagaaaatg aaagaacgcc
    20941 aagagcagat tcgtaatttt tcgatcatcg ctcatatcga ccatgggaaa tctacattgg
    21001 ccgatcgtat tttagaaatg acgcacactg tgacgtctcg cgaaatgcaa gatcagctgt
    21061 tagactcgat ggatttggaa cgtgaacgtg gaatcacgat taaattaaat gctgtagaac
    21121 tcaattacac agcgaaaaat ggagaaacat atatctttca cttgattgac accccagggc
    21181 acgtcgactt cacctatgaa gtctctcgaa gccttgctgc ctgtgagggt gccgttttag
    21241 tggtcgatgc agcacaagga atcgaagcac aaacattggc caatgtttat ttagcgttag
    21301 acaatgattt ggagatcctg ccagtcatca ataaaatcga cctacccgca gcagatcccg
    21361 aacgtgtccg tcaagaaatc gaagacgtaa tcggaatcga tgctagtgaa gctgtattag
    21421 ccagtgccaa atcagggatt gggattgaag atattctcga gcagatcgtg gaatatgttc
    21481 ctgcaccaag tggagactta gatgcaccgc taaaagcttt gatctttgac tctatttatg
    21541 atagttaccg cggtgttgtt ctaaatgtcc ggatcacgga cggtatggtc aaacccggag
    21601 acaaaatcaa actgatgagt aatggcaaaa catttgatgt tacagaggtc ggggtcttct
    21661 cgccaaaagc agttgctaga gactttttga tggtcggtga tgtcggttat atcacagcaa
    21721 gtatcaaaac tgtccaagat acacgagtag gggatacagt aacgttagca gacaatcctg
    21781 ccgctgaagc tttaccaggt taccgaaaaa tgaatccgat ggtttattgt ggtctttatc
    21841 caattgatac ttctcgttac aacgatttgc gggaagcttt agaaaaactg caattgaatg
    21901 atgctgcttt acaattcgaa ccagaaacct ctcaagcatt aggttttggg ttccgctgtg
    21961 gattcctcgg acttcttcat atggatgtcg tccaagaacg cttggaacgg gaattcaatc
    22021 tagaattgat cacgactgca ccatccgtta tttaccatgt taataaaacg gatggtacga
    22081 ctgctacagt agataacccg gcagattttc ctgaacctgt aacgattcag gatgtagaag
    22141 aaccattcgt caaagcacag atcatggtcc caaatgattt tgtcggtgca gtaatggaac
    22201 tttctcaacg aaaacgggga gaattcatta cgatggatta tttagatgat taccgagtaa
    22261 atgtcgtcta taatattccg ctttctgaaa ttgtctttga ctttttcgac aaattgaaat
    22321 caagcacgaa aggctatgcc tcattggatt atgaaatgtc tggttatcaa aaaagcaagt
    22381 tagtgaagat ggacatcttg ctgaatggag aaaaagtcga tgcattaagc tttatcgttc
    22441 accgagactt tgcctatgaa cgtggaaaag caatcgttga aaaactgaaa aaactaatcc
    22501 cacgccaaca atttgaggtg ccaatccaag cagctattgg acaaaaaatc gttgcccgtt
    22561 cagacatcaa agccttgcgt aaaaacgtct tggctaaatg ttatggtgga gatgtttctc
    22621 gtaaacgtaa actcttagag aaacaaaaag aagggaagaa gcggatgaaa caaatcggct
    22681 ccgtcgaagt tccgcaagaa gccttcatgg ccgttctgaa aatggacgag gacgaaccga
    22741 agaaatagca agggtttgtt tgaaaacagt gtaaaaaata aaccatgttt acatggattt
    22801 gccaccggtt tgccaccggt ggtttttttc ggtggcaaaa atcaagaatt ctttctaaga
    22861 ttttccatga tatcgaccgt ttcatttctc atcttatctg ttacatgcga gtaagtatcc
    22921 atcgtaattg aaattctgct gtggcccaat cgttcagaga tttctttcat ttttgcgcca
    22981 ttttcgagaa gaagtgtagc atgagtatgt ctgagagaat ggaagttaaa agagagggag
    23041 agtgcattcg atatcctcct agtattccat ttcactacac ttggcgtaac tagctcaccg
    23101 tcttccttcg tacatactgc atttgaatca atgtagagct ttccatactt cattcgattt
    23161 tctaattgtt gtttcttatg ttttttcaga attgctagca aggtttgtcc aataaaaatc
    23221 gttcgattgg aactgcttgt ctttggcgta ccatataccc atgcgccgtc attctttacc
    23281 atttgtttct ctacagtaat tgttccattt gaaaaatcga catcatccca cgtcagacca
    23341 caaacttcgc caacgcgcat tcccgtataa aatccaatat tcaaaggaat atagaaagga
    23401 tgtccttcag gagtaatttc tagcatatga tcaaagtcct caagagaaat gatttttaga
    23461 tcttttttag tcgttggtcg tgcttcatat tttggtattt ttacatacag cataggattt
    23521 tgcttgatta atccccaagg ataaaccgcc atattcagcg cattcttaag gacagagtga
    23581 gtaatagtca ttgttttctt cgagtaaccc tttttaaatt cagcattgat gaaattttgt
    23641 aaaagagcag gggagagatc cgtaagtttt ttctttccta aataaccgtt tatatgattt
    23701 ttgatggtaa atcggtagtt ttcataggta ttgtatttta gatttagttt aacgtattcc
    23761 tccatccaaa aatcaaggta ttgttttact cgagtatccg tacctaaaaa gtattgtcct
    23821 gtttcgtcaa tatctgataa aacttttcgt aaagcagctt cggcctctgg tcgggtgtct
    23881 ccgccaactt tctccacttt ttttcttgag ccatcatcat tgatatct
//
LOCUS       DAIGOK010000031        23880 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.031, whole genome shotgun sequence.
ACCESSION   DAIGOK010000031 DAIGOK010000000
VERSION     DAIGOK010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.031"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(53..787)
                     /locus_tag="NOA01_000995"
     CDS             complement(53..787)
                     /locus_tag="NOA01_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCI0632207.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="NOA01_000996"
     CDS             1114..1524
                     /locus_tag="NOA01_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCI0632208.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="NOA01_000997"
     CDS             1517..2218
                     /locus_tag="NOA01_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HCI0632209.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="NOA01_000998"
     CDS             2218..3804
                     /locus_tag="NOA01_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCI0632210.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="NOA01_000999"
     CDS             3823..4044
                     /locus_tag="NOA01_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HCI0632211.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="NOA01_001000"
     CDS             4041..4802
                     /locus_tag="NOA01_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HCI0632212.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="NOA01_001001"
     CDS             5182..5691
                     /locus_tag="NOA01_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCI0632213.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="NOA01_001002"
     CDS             complement(5766..6305)
                     /locus_tag="NOA01_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HCI0632214.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="NOA01_001003"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="NOA01_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCI0632215.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="NOA01_001004"
     CDS             7318..8169
                     /locus_tag="NOA01_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCI0632216.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="NOA01_001005"
     CDS             8197..8832
                     /locus_tag="NOA01_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HCI0632217.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="NOA01_001006"
     CDS             8852..9625
                     /locus_tag="NOA01_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCI0632218.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="NOA01_001007"
     CDS             10033..10830
                     /locus_tag="NOA01_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HCI0632219.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="NOA01_001008"
     CDS             10808..11221
                     /locus_tag="NOA01_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCI0632220.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13847
                     /gene="mutS"
                     /locus_tag="NOA01_001009"
     CDS             11205..13847
                     /gene="mutS"
                     /locus_tag="NOA01_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCI0632221.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAQGYIDALIEKGYKVAICEQVEDPKTTKGMV
                     KREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDEE
                     AVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVDP
                     LEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKKH
                     GTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSALT
                     KVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPMD
                     HLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQETG
                     IKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILEA
                     EEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELVS
                     DKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLALT
                     VLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPNS
                     LILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNVH
                     VGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGHH
                     TVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIESL
                     KQINLLEMTPMDALNTLYELQKQL"
     gene            13865..15973
                     /gene="mutL"
                     /locus_tag="NOA01_001010"
     CDS             13865..15973
                     /gene="mutL"
                     /locus_tag="NOA01_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCI0632222.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15995..16555
                     /gene="maf"
                     /locus_tag="NOA01_001011"
     CDS             15995..16555
                     /gene="maf"
                     /locus_tag="NOA01_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HCI0632223.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16640..18628)
                     /gene="treP"
                     /locus_tag="NOA01_001012"
     CDS             complement(16640..18628)
                     /gene="treP"
                     /locus_tag="NOA01_001012"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HCI0632224.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18851..19564
                     /gene="treR"
                     /locus_tag="NOA01_001013"
     CDS             18851..19564
                     /gene="treR"
                     /locus_tag="NOA01_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HCI0632225.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19641..20087
                     /gene="msrB"
                     /locus_tag="NOA01_001014"
     CDS             19641..20087
                     /gene="msrB"
                     /locus_tag="NOA01_001014"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCI0632226.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20257..20859
                     /gene="ruvA"
                     /locus_tag="NOA01_001015"
     CDS             20257..20859
                     /gene="ruvA"
                     /locus_tag="NOA01_001015"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCI0632227.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20872..21873
                     /gene="ruvB"
                     /locus_tag="NOA01_001016"
     CDS             20872..21873
                     /gene="ruvB"
                     /locus_tag="NOA01_001016"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCI0632228.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21902..22480
                     /locus_tag="NOA01_001017"
     CDS             21902..22480
                     /locus_tag="NOA01_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCI0632229.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22532..23014
                     /locus_tag="NOA01_001018"
     CDS             22532..23014
                     /locus_tag="NOA01_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HCI0632230.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23131..23505
                     /locus_tag="NOA01_001019"
     CDS             23131..23505
                     /locus_tag="NOA01_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632231.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7807 a         4288 c         5123 g         6662 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca caaggatata tcgatgcctt gattgaaaaa ggctataaag
    11461 tagcgatttg cgagcaggta gaagatccaa agacgactaa agggatggtc aagcgcgaag
    11521 tgatccagct agtcactcct ggtacagtca tggacagcaa aggattgtcg gcaaaagaca
    11581 acaatttttt aacagctgta gttagtcaag gaaatgaatt tggctttgct tatgccgatt
    11641 taagtacggg agaattgaag acggctcggt tgcatgatga agaagctgtt ttaaatgaag
    11701 cctctgcatt gcagacaaaa gaactcgtat taggcagtga gatcactgat acccttcgtg
    11761 aacagctaag cgggcgttta gggctagtct tttctcaaca aaatgagatg gaagaaaacg
    11821 cggaatttag ctttttgaca agcgaactag tcgatccgct ggaaaaagaa gcaaccggca
    11881 aactattgac ttatttatcg gtgacacaaa agcggagttt agctcatatc caaaaagcag
    11941 tagaatacca accagatcat tttttgaaaa tggattatta ttcaaaattc aacctagaat
    12001 taagccaatc tattcggact gggaaaaagc acggcactct tctatggttg ttagatgaaa
    12061 caaaaacagc aatgggtggc cgtttactaa aacaatggct agaccgtccg cttatccagg
    12121 caaaacaaat taaagcaaga caagaaatgg tcgcttcttt gctagacagc tattttgaac
    12181 gtatcgattt gcagtcagca ttaacgaaag tctatgatct ggaacgccta gcaggtcgag
    12241 tagctttcgg taatgtcaat gggcgagact tgatccagct gaaaacttct ttagaacaag
    12301 tgccaatgat cagagggttg attgaaggaa tcgatcaagg tcaatggcaa agtctgcttt
    12361 cagaaatgca gccgatggac catctcgtcc aattgatcga ccaagctatc caagatgatc
    12421 cgccgttaca aataacggaa ggaaacatca tcaaagatgg attcaatgag cagctcgata
    12481 tgtaccgttc tgctatgaga aacggaaaga aatggttggc agaacttgaa gcaaaagagc
    12541 gtcaagaaac aggaatcaag aacttaaaaa tcggatttaa ccgcgttttc ggttattata
    12601 tagaaattac aaaagcaaat cttggaaatg cagaactgga gaagtatgaa cgaaaacaaa
    12661 ctttagcaaa tgcagagcgt ttcatcacac cagaactaaa agaattggag acacaaatcc
    12721 tcgaagcaga agaaaaatct gttgatttag aatatcagtt gtttttagct gtgagagaag
    12781 aagtcaaaaa ggctatccag ccgctacaag tacttgcaaa agcaatcagt gcagtagatg
    12841 tgctacaaag ttttgcaacg atcagcgaac gctatcagta tgttcggcca gaacttgtca
    12901 gtgacaagca tcagcttctg atcaaagacg gacgacatcc tgtagtagaa aaagtattag
    12961 gacatcagga atatatccct aacagcgtag aaatggcaga agacgaaatg atcttgctga
    13021 ttacaggacc gaatatgtct ggtaaaagta cgtatatgcg acagttggca ttaactgttc
    13081 tgatggcgca aatgggctgt ttcgttccgg caaaacaagc agttttacct atttttgatc
    13141 ggatttttac ccgaattggt gcaagtgacg acttaattgc tggacaaagt acttttatgg
    13201 tagagatgat ggaagccaat caagccctgc gttatgctac gccaaacagt ttgattttgt
    13261 ttgacgaact agggcgagga acagcaacct atgacggaat ggcgttggcc caagcaatca
    13321 tcgaatatat ccatcgtcat gtacaagcaa aaacactctt ttctactcat tatcatgaac
    13381 tgacggtact tgagaaagaa cttccgcagc ttaaaaatgt ccatgttgga gccgttgaaa
    13441 aagacggaga agttgtcttc ttgcataaaa tgatggatgg acctgcggat aaaagctacg
    13501 gcatacatgt ggcaaagatc gcaggattgc cttccaatct actagaacga gcagcagata
    13561 tcctgcatac gctagaaaat ggcgataacg gacaccatac tgttccagct gaagtagaag
    13621 agaaaactaa aacagccaaa caggagcaaa taaaaccacc tgcagaaaaa gtaaatcaag
    13681 accatataca agaccgtgta aaagaagaaa accaacagct ttctttattc agtgaattat
    13741 ccgaaaatga aacagaagtc attgaatcac tgaagcaaat aaatttacta gaaatgacgc
    13801 caatggatgc actaaacaca ttatatgaat tacaaaaaca actgtaggaa gaaggcggat
    13861 atcgatggcc aaaatccaag aattatctga acgtttagct aatcagatcg ctgcgggaga
    13921 agtagtggag cgaccagctt ccgttgtgaa agaattagtg gaaaatgcaa ttgacgccgg
    13981 cagtactcag attgatatct tagttgaaga agcgggactg cggacgatcc aagtgattga
    14041 taacggagaa ggcattttgg cagacgacgt agagaatgcc ttcaagcggc atgcgacaag
    14101 taagatccat aatcgcgacg atctttttcg catacgaacg ctgggctttc gaggggaagc
    14161 tttgccaagt attgcctctg tttctgaaat gactctggaa acggcgaatc aggaagaaaa
    14221 acaaggcacc tatctgtcgc tgaaaggcgg agaagttatt gagcatcgac cagcaccact
    14281 gcgtcaagga acgaagatta ccgtcagcaa tctgtttttc aatacgccag cccgcttgaa
    14341 atacgtgaaa acactgcaca cagaattagc gaacatcggg gacatcgtca atcgactggc
    14401 attgagtcat ccatcgattg cttttcgttt agtacatgat ggaaacaaaa tgctggcaac
    14461 tgctggaaat ggcgatctga aacaaacaat tgcgggaatc tacggattat ctaccgcaaa
    14521 aaaaatgctg aaaatcgaag gaaaagatct agattttgag ttatttggct atgtttcatt
    14581 gcctgaggtg actcgtgcca gtaggaatta cttatcgacg atcatcaatg gacgttttat
    14641 caagaacttt tccttgaata aagcaatcgt tgcaggttat gggtcaaaat taatggtcgg
    14701 tcgtttccct attgctgtat tggaaatcaa aatggacccg cttttagtag atgtaaatgt
    14761 tcatccgacg aagcaggaag tccggctgtc gaaggaaaaa gaactaacag agctaattag
    14821 taacgccatt caaaaagcat tgtctcaaga aaatctgatt ccaagtgcag cggataatct
    14881 acggtttaag aaaaaagtac ctgatcagcc taaaacagaa caaatcgaaa tcgatttgtc
    14941 ctacgctttt gaagaagaaa aacctaaaaa gccaagtagt ttgaattttg atcgagagac
    15001 aggtaccttt ttcgtagaag aactaaagaa cacatttgtt gacagaattg ttgataagtc
    15061 tgtggaaaat gttgaaaact attctggttt ttctgaagaa aacaccactt atccagaaaa
    15121 aacagcgaaa acggtggata agtttggtga aaatgttgat aactttgtag aaaaaacagt
    15181 ggaaaaatct gaaaagaaag aatttcccat tgtggataac atggagaaaa caaatgaaga
    15241 acaagcagat aaatccgtag aagaggatga aaagatttac gaggaagaat tgacactcca
    15301 tcctgaattt gatttcactc aaggaaacga caaaacatta gaacaagcca tcaagaaatt
    15361 agaaacggaa aaaccagtac aacgttttcc tcagttggaa tattttggac agatgcacgg
    15421 aacgtatctt tttgcccaaa gtagcgatgg gttgtacatc attgatcagc atgctgccca
    15481 agagcgcatt aagtatgaat atttccggga aaaaattggg gaagtatctg atgatctgca
    15541 agaattgctt gttccatttg tgttagacta tccaaacagt gatgctttga aattaaaaga
    15601 gcaaaaacac ttgctagaag atgcagggat ttatttagaa gaatttggtc aaaacagctt
    15661 tattgtacgg gcccatccta cttggtatcc caatggagaa gaagaatcga ttattcggga
    15721 gatgatcgat atgctgctga caactggaag tgtgagtgta aaaaaattcc gtgaagcaac
    15781 agcaatcatg atgagttgca aacgatcgat taaagccaac cattatttga atgaagcaca
    15841 agcacgtgtg ctgctgaagg atctggcacg atgcgaaaat cctttcaatt gtccccatgg
    15901 ccgtccagta ttgattcact ttaccaattc ggatatggaa aggatgttca aacgaatcca
    15961 agacccgcat taagtcaaag agaagaggga aaacatgaag attgtattag catcccaatc
    16021 accaagaaga aaagagttat tggctcatat cgtttcagaa ttcgagattg aacctgctga
    16081 tatcgatgaa acgccatttc cagacgaaca gcccgtcgat tatgttcgta gaatggctga
    16141 agaaaaagca cgaactgtct gggaacaaga tgaaaaagaa gaccatttag tgattgccag
    16201 tgacacgact gttgttctca atcaagagat catgggtaaa ccagaagatc tggctgaggc
    16261 tgaattcatg ctgaaaaaac taagcgggga aacacatact gtatatacag ctgttgtttt
    16321 aaaaacaaag gaacgagaag aacggatact agcagaagca catgtcactt tttatccttt
    16381 gacagacgaa gagatcaaac gctacttgga gacgggggat tatgcggata aggcaggggc
    16441 atacggcatc caaaatgctg cgagtgtatt cgtcaaagaa atttctggag attattacag
    16501 tatcgtagga tttccgattg gtgctgtcaa tcaggcactg aaaacattca tttaaacaaa
    16561 aaagcggact atctgatttt cgattgaaaa tccatgatag cccgtttttt ttattgaaaa
    16621 gattacttat ctactctgac taagatttta cttcagcttt accgactgct tcgcccgggc
    16681 gaacaagtcc tgattggtca agagaaagtc ctgcaacttt ttcattattg gtcaaagcaa
    16741 caataatgtc agttttcttg ccagcttgtt ctatttctgg caaatccatt tcagcaatca
    16801 gggtctctgg tgtgacaagg tatccttctt tcacgaatac attgaaagga acacccttta
    16861 gttcaacagt atctaatccc atgtgaacaa gtacttccag cccctcatta gaaagaatac
    16921 caatggcatg ttttgtttca aagacactcg tcactttacc gtttactggt gcatagactt
    16981 tgccgtttga cggttcgaca gcataaccgt cacccatcat tttttgtgag aaaactgggt
    17041 catttacttc agttatttcc ttgatggtcc ctgcagcgac tgcgaataat gtttcttttg
    17101 ttgcaactgc cgtaccagtt gctgaaacag gttcgaaaga ctgtggcgtt gctgttgcgc
    17161 catttgctgt ttgaagttgt acatcagcag cattttcagg aacagcagga tcaatcttat
    17221 tcaaaattcc ctgacgtcta aagatgatcg tcaagccaaa tggaatgatg acagcaatga
    17281 tcattgcgat gatgaaaggt acccatgttt ctgcttggat agctagtatt cctggcaaac
    17341 cgcctactcc gatcgcgtta gctcgaacac ccatcaagtt agcaaacatg ccagctaatc
    17401 cagagccgac cattgcagcg acgaaaggat agacgtattt caaattgatt ccgaacatag
    17461 caggttcagt tacgcctaag taacaagaaa tcattgctgg aatcgagatt tgttcttctt
    17521 ttttattccc acgatgtaag aagatgatgg ctaagactgc agagccttgg gcgatatttg
    17581 ataaagcaat catcggccat aaattcgttg atccgaaatc tgcaatcagc tgcatatcga
    17641 tggcattggt catatggtgg agaccagtaa tcactaaagg agcatacagg aatccgaata
    17701 cggcactaaa caaccagctg atagaagacg tcaatccagt atttacgata tttgagatcc
    17761 agcttccgac tgtccagccg ataggaccta aaataacgtg tgcagctaaa acagttggta
    17821 acaatgaaaa caatggaaca aaaatcatcg agatcgactg agggatatat ttgcggaaga
    17881 agatttctag atacgctaac ataaaccctg ccagcatggc tggaataact tgcgcttgat
    17941 aaccaatcat ctggacttga gcaaaaccaa aatcccaaaa tggaatatct gctgcggctg
    18001 tggaagctac actataagca tttaataatt gcggagaaac taacgtaatt cctaaaacga
    18061 ttcctaatat ttgtgtagta cccatctttt ttgcaatact ccaagtgata cctactggaa
    18121 gaaaatggaa aattgcttca cccggtaacc acaaaaaggc attgacacca ttccaaaact
    18181 tcgagtgttc aacgattgtt ccaccaagac tttcaaactg gataccttca agtacatttc
    18241 tgaatccaag gatcaatcca ccgacaatga ttgcaggaat cagtggtgta aagatttctg
    18301 ctaaaacggc aatagcacgt tgaacgggat gaaggttttg ttttgcagct gcttttcctt
    18361 gttctttgga aaccccttca actccagata ctgcaacgaa gtcgttataa aaagtagaaa
    18421 catcgtttcc tatgatgact tggaactgtc ctgcattcgt gaacatccct tttacgctag
    18481 gtatatcttc gatcgctttt tcgtccgctt ttcctggatc attcaggaca aaacgcatac
    18541 gagtcgcaca atgactaact gcagcaatat tttcttttcc gccgatatca tgtaacagct
    18601 tctcggcatc tgcttgatat tttgccatat caataaaccc ctttctctta acttgtctat
    18661 gcaagttgtt tcttcattta ctatactttt tttcggagag aattgcaagc gttttattaa
    18721 aaagtacgag agaaaatgat atcgttttaa aaagcttaaa ataagctttt cttaaaaata
    18781 ggaacattga aatacaaaaa gagctgtagt atagtgaaga aatgatggaa gaacgagtga
    18841 gggaaaaaac atgaataagt tcaatgaaat ttttttagat ttggaacaga aaattttagc
    18901 gaaagaatat cctccgcatt cgcttttacc cagtgagaac caattgatca aaatttatgg
    18961 tgtttcacga gaaacaatta gaaaagcgct gaatcttttg actacggaag gttatattca
    19021 aaaaaaacaa ggaaaaggtt cgatcgtact ggatcggaat cgcttcgact ttcctatttc
    19081 tggattgact agctataaag agctacagga gacccaacgt attccaagtg aaacgatcat
    19141 ccatacgtta gaagaaacag aagtgacaaa agcattgaac gagatcactg gctgggagat
    19201 cggtgcgcct gtttggcatt tgatacgcga aagaaaaatc aatggcgaag tagttatatt
    19261 agacaccgat tatctattaa aggaaatcgt tcctatttta actccagcac aagcacaagg
    19321 atcgatctat gaatattttg aaaatgagct ttctttgacg attgcttacg cgcaaaagga
    19381 gatcacagta gaagaagtaa cggaaatcat gaaaacagcg atggatcttc acgatgacac
    19441 ttatgcagta gtagtcagaa gcctcgttca tctggaagat acccgctgtt tcgagtattc
    19501 tgagtcggtt caccgattag ataaattccg tttcgttgag tttgcccgac gccgaaaagt
    19561 gtaaatagtt caaaagaaag caaattgttg gcatctgtcc actttggcgc tatgatagga
    19621 tcacaaaggg ggcattttta atggataaac caataagacc agaaaaagaa gaactgaaag
    19681 aaaagttatc acccatggct tatgcagtca cacaagaaaa tgcaacagaa cgaccattta
    19741 gcgggaaata tgatgatttt tacgaaaagg ggatctatgt agatatcgtc agcggtgaac
    19801 cgttattcag ctctgcagaa aaatatgatg caggttgtgg ctggccatca ttcagcaaac
    19861 caataacgaa aagacaagtg cgcgaaaaag ccgatttttc tcatggcatg catcgaatag
    19921 aagttcgaag caaagaagca gattctcatc tcggacatgt gtttaccgat ggaccagtag
    19981 atcaaggtgg tctacgttac tgcatcaatt ctgcctcact gaaattcatt ccatatgaaa
    20041 agatggatga attaggttat ggggaattca aatcacttgt agaataatgg aaatacacaa
    20101 agagcggtac aatacgggga tttactcgat cgtccgctct ttttttattc aagcgattgc
    20161 tatttgaaag aaacgctccc tagactcctt ttttcacaag gtgaaatgtg ctacaataaa
    20221 gggaacgtat gtgtgcaaaa gaaagaggac tagaatatgt atgaatatct gacaggtgtg
    20281 gttaccttta tcaaccccta ttacatagta gtagaaacca atggtatcgg ctaccagatc
    20341 gcactaggaa atccttaccg ctacagtagc aaactaaatc aaaaaataaa aatttatgtc
    20401 catcaggtaa tacgtgaaga tgcgcatctt ctgtatggat ttgattcgct agaagaaaag
    20461 caactgtttt tgcgattagt aagtgtttca ggaatcggtc cgaaaagtgc ccttgcaatc
    20521 atggcaagcg aagaccactc cggtttgatt caagcagtag aatccagtga tgtcacttat
    20581 ttgacgaaat ttccaggagt agggaaaaag acagcacaac aaatgatctt ggatctaaaa
    20641 ggaaaattcg gtgagctgag tatcgataca ccttttagtc tatttgatga agcaaacaca
    20701 aaagacgcta cagctctatc tgaagcaatg gaagctctct cggctcttgg ttacagtgat
    20761 cgagaaatca aacgtgtaga aaaacaatta aaagaagtag ataatcagac aacggatgag
    20821 tatctacgcc aagcactcaa gctaatgatg aaaaaataag gaggagacca aatgacagat
    20881 gaatacttgt tatcaccaga atctggcgaa aatgaactga gtttggaaaa atctcttcga
    20941 ccacagctgc tttctcaata tatcggacag agaaaagtca aacaagaact tcagatttac
    21001 atcgaagctg ccagaaatcg ggaagaacct ttagatcatg ttctgttgta cgggcctcct
    21061 ggattaggaa aaacgacgat ggccatggtc attgctaatg agatgaccgt aaatattcgt
    21121 acgacgagtg gccctgctat cgaacgtccg ggtgacttag tagcaatctt gaatgaactg
    21181 gaagcagggg atgtcctgtt tatcgatgag atccatcgat tacctagagt ggtagaagag
    21241 atgctttatt cagctatgga agatttctat gtagatatca tggtaggaca agggccaacc
    21301 gcacatccgg ttcatttccc tcttcctccc tttactttga ttggcgcgac gacacgggca
    21361 ggtatgcttt ctgcaccttt acgagatcga tttggcatca tttctcatat ggaatattat
    21421 gacgaacagg atctgcagga gatcgtttta cgttcttcgg atatttttca aacagagatc
    21481 attgaagaag gggcattgga aattgctcgc cgatcacgag gaacgcctag aatcgctaat
    21541 cgcttgctca aacgggttcg tgattttgcg caagtccaag gagacgggaa aatcgatcgt
    21601 cctatagcgg atcaggcact tactttgcta caggtcgatc aggctggatt ggattatgtt
    21661 gaccagaaat tgttgcgaac aatgatcgag ttatatggcg gcggaccagt agggttaagt
    21721 acgctttcag taaacatagg agaagagcgt gagacggtag aagatatgta tgaaccgtat
    21781 ctgatccaaa aaggattttt aaagcgtaca cctagagggc gaattgctac tgcttatgcc
    21841 tatgaacatt ttggttacga ctatcatata tgatttttgc ttttagattg gaggaaaatg
    21901 aatgagtgat acaacacgta cgaaacaatt gatcaaaaac gtattgattg atttgtgcca
    21961 aacgcaatct tttcgaaaaa tcagcgtcca aaaaatcgca aacgaagcaa aaatcaatcg
    22021 acaaacattt tattatcatt ttacagataa atacgatttg cttcgttggg tctattgtga
    22081 ggatgcattg cattatttag atccacaatg tctttcactt gataattggg aagagcaagc
    22141 attgaaaatg ctgaaagcaa ttgctgagaa gaaagaattt tatgcaaata ctgtgctttc
    22201 tgacccagaa attttacaaa aagagttttc tcaactgatc caaaaatcat ttatcaagat
    22261 ttttgaacaa atggatgaag aaagacaact ttctgcaaga gacaaactat tttatgctcg
    22321 ttttttttct tacggttgca gtggtgtatt agtcaattgg atcacgaatg aatatgaaga
    22381 aacaccgatg gaaatcgcta tgcagctttt ccgtttagcc aaggatacgg aattatattc
    22441 ttatcgtctt tatgcaaaag aagagttgga agaacagtaa tagaaaaatt ttttgttgga
    22501 aaagcaataa tcgctaaaag gagatgggga aatgacaaaa attttatttg tttgtttggg
    22561 aaatatttgc cgttcaccga tggcagaagg cttgctgaga aaacgaatag cattagaagg
    22621 aagggaagca gaattttttg tggattctgc tgctacaagc acgtatgaag ttggaaagac
    22681 accgcaccct ggaacgaaaa aaatattaaa ccaagaaaaa gtagatatga cgaatatggt
    22741 tgctcggcaa atcacacccc acgattttga aacttttgat tggatcattg gcatggatca
    22801 agaaaatgta gaagaactga aacgaagagc accacgctca gcgcagggga aaatccattt
    22861 gtttttaagt agcattcctg gaaaagaaaa agaaaatgtt cccgacccat actataccaa
    22921 taactttgat gagacatatc acttgatcac agaaggcctg aatcattggt ggaatatttg
    22981 gattgatgaa aataaaataa caaataattt gtaaagttaa cgctttcttt ttaaggcgat
    23041 gttagagcat ttccttgttt ttttgtaaaa tgttaggtaa aataaaatta attaagtaac
    23101 actttcttag ctaaaggagg agtgcattcg atgaacgaag agacagtatc gccaatgaag
    23161 aagcttacga agttagttac tcatacttcg tatcaacttt ttgattcttt tgtaaatgac
    23221 actgaagaac atgctaagaa tgtacgattg caaggaaagt ttattcttga gctgcaaaaa
    23281 gctgctactg aacatagtct agttatctta caagtaagag caaatcgttt gagtagcaag
    23341 tatgaaacga tctcaggctg ggtggcgaca aaaacggtcg aaaaagaccg agtgatgatc
    23401 cgcctacaaa acgaagagca gcagatccgg atggttcctg tagaacaaat tgaaaaaata
    23461 acaatccttt caccagcagg ggaacaggaa cggttatcaa aataatttat accagataga
    23521 aaagcacgta tgagcttgta ccatcaatca ttactaatca aaaataaccg aatgctttaa
    23581 gctgatatca gtaaaacgga acgttttagg atgagtatca tagcatgaca gcattcggtt
    23641 gattagttga atcgattgga tggtacaagt ttttttgata aaaaaaggtc ctgctagccg
    23701 gggagctagc aggataagga gttaaaaatg aaaaagtgtt gttagggttg tttgttggta
    23761 tgcttatata atagtcatta gttgtgaata ttttgtgacg aaagttttcg aaagttgtta
    23821 agtttttgag agtataaaat attttgtgta aatgaaaaaa tccatacaaa aaaggaagtc
//
LOCUS       DAIGOK010000032        23681 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.032, whole genome shotgun sequence.
ACCESSION   DAIGOK010000032 DAIGOK010000000
VERSION     DAIGOK010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.032"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            299..1960
                     /locus_tag="NOA01_001020"
     CDS             299..1960
                     /locus_tag="NOA01_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCI0632232.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2377)
                     /locus_tag="NOA01_001021"
     CDS             complement(2030..2377)
                     /locus_tag="NOA01_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HCI0632233.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2461..3402
                     /locus_tag="NOA01_001022"
     CDS             2461..3402
                     /locus_tag="NOA01_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI0632234.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3403..4446
                     /locus_tag="NOA01_001023"
     CDS             3403..4446
                     /locus_tag="NOA01_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI0632235.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4452..5522
                     /locus_tag="NOA01_001024"
     CDS             4452..5522
                     /locus_tag="NOA01_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0632236.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5512..6465
                     /locus_tag="NOA01_001025"
     CDS             5512..6465
                     /locus_tag="NOA01_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCI0632237.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6477..7487
                     /locus_tag="NOA01_001026"
     CDS             6477..7487
                     /locus_tag="NOA01_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCI0632238.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7772..8593)
                     /locus_tag="NOA01_001027"
     CDS             complement(7772..8593)
                     /locus_tag="NOA01_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCI0632239.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8690..8968)
                     /locus_tag="NOA01_001028"
     CDS             complement(8690..8968)
                     /locus_tag="NOA01_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632240.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9236..11245
                     /gene="metG"
                     /locus_tag="NOA01_001029"
     CDS             9236..11245
                     /gene="metG"
                     /locus_tag="NOA01_001029"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCI0632241.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11724..12311)
                     /locus_tag="NOA01_001030"
     CDS             complement(11724..12311)
                     /locus_tag="NOA01_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632242.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12486..13808
                     /locus_tag="NOA01_001031"
     CDS             12486..13808
                     /locus_tag="NOA01_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCI0632243.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13963..14739
                     /locus_tag="NOA01_001032"
     CDS             13963..14739
                     /locus_tag="NOA01_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCI0632244.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14732..15301
                     /gene="rnmV"
                     /locus_tag="NOA01_001033"
     CDS             14732..15301
                     /gene="rnmV"
                     /locus_tag="NOA01_001033"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HCI0632245.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15294..16178
                     /gene="rsmA"
                     /locus_tag="NOA01_001034"
     CDS             15294..16178
                     /gene="rsmA"
                     /locus_tag="NOA01_001034"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCI0632246.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16629..17258
                     /locus_tag="NOA01_001035"
     CDS             16629..17258
                     /locus_tag="NOA01_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632247.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="NOA01_001036"
     CDS             complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="NOA01_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HCI0632248.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18700..19122
                     /gene="mgsA"
                     /locus_tag="NOA01_001037"
     CDS             18700..19122
                     /gene="mgsA"
                     /locus_tag="NOA01_001037"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCI0632249.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19445..19981
                     /locus_tag="NOA01_001038"
     CDS             19445..19981
                     /locus_tag="NOA01_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HCI0632250.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20021..21784)
                     /locus_tag="NOA01_001039"
     CDS             complement(20021..21784)
                     /locus_tag="NOA01_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0632251.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21787..23502)
                     /locus_tag="NOA01_001040"
     CDS             complement(21787..23502)
                     /locus_tag="NOA01_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0632252.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7642 a         4323 c         4804 g         6912 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgaggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt ttttcatttg attgctcctt tatttttggt
     2401 tcattatcta ataatgtagc gaaacatctg tcagaagtca aaagctaggg ggaaacatta
     2461 gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg
     2521 ttaattgcaa caatcacctt tttcttgatg caattgctgc cgggcacgcc atatacgaat
     2581 caggaacgtt tgagtcctga aacaatcgag atgctaaata aacaagtcgg tttggataaa
     2641 ccagttatcg tacaatatgg gatttatctt tctaatttgc ttcaaggaga ttttggaatt
     2701 tccttccaat ttaaaaacca gccagtcgca catttgttag ctggaagaat cggaccatcc
     2761 ttgcagttag ggttacaggc aattatcttt ggaacagtcc tagggacgat tttaggaacg
     2821 atctctgcca tgaagcaaaa tacatgggca gatacatctt ctacactcgt agcgatatta
     2881 ggtcgttcca ttccaaactt tgtatttgct gttttattac aatatatctt tgcaatcaaa
     2941 ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt acacaatctt accaacaatt
     3001 gcgttggcca tgtcgccgct tgctgactct gctcggttta tccgtacgga aatggtggaa
     3061 gtactgcata gtgattatgt ggaattagca aaagcaaaag gattgagccg ttgggaaatc
     3121 gcctttaaac atggtcttcg aaatagtttg attccattga tgactttatt aggaccgtta
     3181 gctgtagcat tgatgaccgg atcactggtg gtagaaaata tctttgctat tcctggtatc
     3241 ggggaacaat tcgttaagtc aatcacaaca aatgattatc ctacgatcat ggctgtaacg
     3301 atcctttact catttatgct gatttttgtt attttgatcg tggatctatt gtacggtcta
     3361 gttgatccaa gaattcgtgt ttcagaaggg agccgaggat aaatggaaat gataccaaag
     3421 cgttatgaaa cagttgcaga tatccctgcg gatgaatttc ttccattaca aaaaaatacc
     3481 gaagaagaac gtgaacagat tactgcacct tctttgaatt ttatccaaga ttcatggcgc
     3541 cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt tactattggt catcattttg
     3601 atctctgtga tcacaatctt tgtttctcct catgatccaa ctgcccaaaa cgtggcttat
     3661 atcaatttgc ctcctcgtat tccgggaatc aatatcgatg gattgaatgg aaaagcaatg
     3721 gttggtggtg aacttgttga taaatatgct caagcaaacg tcccttcaaa cgtaaatttc
     3781 tttttaggaa cagacggttt gggacgtgat gtattaagcc gcttatttat gggaatgaga
     3841 atctcgttgc tgatcgcatt tattgctgca ttactagatg taacgattgg tgtagcttac
     3901 gggttggttt ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg ttttttggaa
     3961 atcttatcag gaattccaaa cctcgttgtc atgattttga tgttggtcgt ttttgaacca
     4021 ggaatttttt caatcgtggc tgctatggcc atcacaaact ggataccaat ggcacgtatc
     4081 gttcgtgcac aaacaatgaa attaaaagat caagaatatg ttttagctgg attgacatta
     4141 ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc cgaatatctc tagtgtcatt
     4201 attattcaaa tgatgtttag tattccatct gctattttct tcgaagcctt tttaagcttt
     4261 atcggtttag gtcttacgcc tccgtcagct tcattaggaa cgatgttgag cgaagggtac
     4321 aagacattcc tgtatctgcc ttatctatta tggattccgg cagtaacttt atcggttatc
     4381 atgattggct ttaacttact tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa
     4441 gagtgaagac gatgacgaaa attttagaag tgaaagactt agaaatctct tttgatacgt
     4501 atgctggaaa ggtacgagcg attcgcggtg tcaattttca tttgaataaa ggagaaacat
     4561 tggcgatcgt tggggaatct ggaagtggaa aatcagtgac gaccagaagc atcatgcgct
     4621 tgctgtcaag caacgccaat atagatgctg gtcaaatcct ctttaaaggt aacgatatcg
     4681 ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc
     4741 aagatccaat gacctcttta gatccgacga tgcccattgg gaagcaagtc gctgagtccc
     4801 ttagaaagca taataaagtt tcaaaaaaag agggacaaaa agctgcatta gaattgctta
     4861 aattagttgg aatacccgaa gctgagaaac gtatcaacag ttatccgcat cagttctcag
     4921 gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt atgttatccg gaaatcctga
     4981 ttgctgatga acctacaaca gcgcttgatg tgactattca agcacagatt ttggagctgc
     5041 tgaaagaaat tcagacaaaa atcgatacat caattatctt tatcactcat gaccttggtg
     5101 tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg
     5161 gaacatcgga agaaattttc tacaacccgc aacatcccta tacttgggga ctgcttggtt
     5221 caatgccgac attagatagt gcgaacgatc gattgtatgc tattcctggt tctccaccgg
     5281 atttattgaa cccgccaaaa ggagacgcat tctatccaag aaatgaattc gcgatgaaaa
     5341 tcgatgcaga acaagaacca ccattttttg aactatcaaa aacacacaag gcagcaactt
     5401 ggctacttgc tccgcaggca cccaaagtga caccgccaga agaaatccaa cgtcgctggg
     5461 ctatctttaa agagaaacaa aaacatactt acggtcaagg aggaattgct gatgccaaat
     5521 aaactacttg aagttaaagg attgaaacag tatttcaatg taggaaaaaa gaatgaagta
     5581 catgctgtaa atgacatcag ctttcacatt tatgaaggag aaacattcgg tttagttgga
     5641 gaatctggaa gcggaaaatc cactactggc cgtacgatca ttcgcttgaa tgaacctact
     5701 gatggtgaga tattattcga cggtcaagat ataacgaaga tcaaagataa aaaaggtttg
     5761 acgaaattcc ggcatgatgt tcagatgatc tttcaagatc catacgcttc gttgaatcct
     5821 cgtatgaaag tcagagatat cattgccgaa ggaattgatg ttaatggact agctaaatca
     5881 tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt gaatccaagc
     5941 cacggtactc gttatcctca tgaattttca ggtgggcaac gccagcgtat tggtattgca
     6001 cgagcattgg cagtaaaacc gcgttttatc atttgtgatg aaccaatttc tgcactagat
     6061 gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg
     6121 acctatctat ttattgcaca tgatttgtcg atggttaaac atattagtga ccgaatcggt
     6181 gtaatgcata acggcttgtt attagagatg ggaacaagtg atgaaatcta taatcacggt
     6241 gttcatcctt atacagaaag tttgttatct gctattcctc taccagatcc agaccatgag
     6301 agaaaacgca gaagaatcaa atatcaacct gaaccagatg acggacaagt acgtcaactt
     6361 cgtgaaatcg caccagaaca ttttgtctat gcaactgagc aggaagtccc atattacgca
     6421 aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta actaggaggg acatagatgc
     6481 taaaagcttt taaattccgt atgtatccga cagaagaaca aaaacagcaa ttgattcgta
     6541 cttttggatg tgctcgattt acctataacc atttgctgaa aaagcggcag aaaagttggc
     6601 aacaaacagg agtagccaat ttttcgctca cacccgctac attaaaaaaa gaatacccat
     6661 tcttaaaaga agtagacagt cttgctttag caaatgcaca gcttaatttg gatcgggctt
     6721 ttcgcaatta tttcaaggga cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt
     6781 ggcaatctta tacgaccaac aaccaaaccc gtacgatcta tctggaaaat ggctacttaa
     6841 agttaccaaa acaaaaagaa ctaatcaaga taaatcagca tcggccagtt gaaggatcga
     6901 ttcgctccgc tacgatttct gctcgatata atgaagaatt ttatgtcgca ctactttgtg
     6961 atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc tatcatccga
     7021 agacattgat ccaaacatct cgacctctag aagtaacttt accaaaattt caccagacag
     7081 aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc gctcaccata
     7141 gaaagatcag gttagatcag gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc
     7201 tttatctgaa acaaaagaac cagcgagaag attatcttga acaattatct gggaaattgg
     7261 tcaaacaata tgactactta tttgtagaat cttttccaaa agaagaggca catgctgatt
     7321 tttctattca cgactggcat aaattaataa caaaattgcg ttataagtcc caatggtata
     7381 ataaaaaatt tcttttgatc aataccgacg gagcagaaga aagcaattcc gtaagaaaga
     7441 gccaagtagt agaaaaaatg ggcagacatt cggttatcaa gggataaaaa atgaatgaaa
     7501 ctgataggag cgttagggtt agccttgtta ataaaaagtc ttaatgactt ttgtttctag
     7561 gaatttttct tttgaagaaa agctcactaa ttatgacaaa ccaataaaac aatagcagaa
     7621 aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac
     7681 atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga
     7741 atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat
     7801 agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga
     7861 gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag
     7921 caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg
     7981 gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt
     8041 caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca
     8101 cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt
     8161 ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg
     8221 tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat
     8281 tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag
     8341 ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc
     8401 cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta
     8461 cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt
     8521 taagaaattc ttcttcactt gctttatgtt cataatataa tttttcttct tctttagaag
     8581 caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc
     8641 agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt taaatatgaa
     8701 aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat
     8761 accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac
     8821 gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat
     8881 acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa
     8941 gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt
     9001 ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca
     9061 aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga
     9121 tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta
     9181 tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc
     9241 agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca aacttcatat
     9301 cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg
     9361 ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc
     9421 agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa
     9481 attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca
     9541 taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg
     9601 tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc
     9661 agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga
     9721 agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct
     9781 attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat
     9841 gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac
     9901 ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt
     9961 atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata
    10021 ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg
    10081 gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg
    10141 gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat
    10201 gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt
    10261 tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc
    10321 aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg
    10381 actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc
    10441 tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt
    10501 ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg
    10561 ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc
    10621 tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa
    10681 gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg
    10741 cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga
    10801 tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga
    10861 agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat
    10921 caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa
    10981 agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg
    11041 tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa
    11101 agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat
    11161 gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat
    11221 gccaaatggc tcagaaatcg catagaaatt caaagggggt atgacactag ttgtgtcata
    11281 cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc
    11341 aatcatataa tacagaaaca gagaactatc caaaataaat cctagaaaaa gaagtacgag
    11401 cttcgctgat ttttgttaat ttagaataat actaatagga atcgtaaaaa cagaaaaaaa
    11461 gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct tttttattgt
    11521 ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta tatctaacag
    11581 tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt tgtgctctta
    11641 tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc gatgattgtt
    11701 ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat ttttgtcttc
    11761 aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac ataccagaaa
    11821 atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg taacaagatc
    11881 aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat gacttcacta
    11941 agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt catccgccag
    12001 ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa aataccgata
    12061 ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat cacaggacct
    12121 ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg tgataacgtt
    12181 gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga ctgctgttgt
    12241 tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg ataagaacgc
    12301 ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa
    12361 aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa aaaagaatgg
    12421 taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga agaaagagga
    12481 tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta
    12541 tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact gctagtactc
    12601 agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg atctgtgctg
    12661 gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct gtaaaatata
    12721 ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa agcttgattt
    12781 actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg acgaacttat
    12841 tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt tctgtgacag
    12901 ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtgacg ttgattgtta
    12961 aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga caaggaactg
    13021 tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga ttgagcagtg
    13081 cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca atggcagggg
    13141 aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg agttttgtaa
    13201 cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt gatcatcttg
    13261 ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga atcggtggaa
    13321 agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt tatacactaa
    13381 ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc agtaaacagc
    13441 tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc cagttagcag
    13501 tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg ttgatttttg
    13561 tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga aaaaaagcgc
    13621 cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt attgcgattc
    13681 tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta gcattgatcg
    13741 gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa aaacgctcag
    13801 aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc tagatcttcg
    13861 tgctttttct atttcaataa aacaagttct tctattttga tttttgctac ttctgcaagt
    13921 agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt tgattctcat
    13981 acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca gcgagcacaa
    14041 gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct
    14101 ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca tccgacagag
    14161 gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac tttaccaaag
    14221 gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc gaaagaagtc
    14281 caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct accaatcagt
    14341 attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga agatgttcga
    14401 gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa acgatttctt
    14461 gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc attagacgtc
    14521 caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga tgccccttat
    14581 ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg ttatacagta
    14641 gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca aacatggaca
    14701 aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc aagagattat
    14761 agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg cagatacgat
    14821 agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac atgcccaaga
    14881 gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat
    14941 cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc
    15001 aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt tagaagcatt
    15061 gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca ctagacaaga
    15121 ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag aaaaactagg
    15181 tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt
    15241 tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac aacatgagtg
    15301 aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa catggttttt
    15361 catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta aggaaaatcg
    15421 tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca ggaatcggtg
    15481 cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag atcgatgatc
    15541 gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact attgtacatc
    15601 aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat gaggagcttc
    15661 cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg atgcattttc
    15721 ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc
    15781 gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct gttcaatatt
    15841 atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct cagccaaatg
    15901 tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg
    15961 aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa acgctttgga
    16021 ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta gcaaaaagct
    16081 tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta caagaattcg
    16141 ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt
    16201 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcatg
    16261 tttttcgttt gtttgggctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    16321 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
    16381 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
    16441 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
    16501 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
    16561 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
    16621 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
    16681 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
    16741 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
    16801 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
    16861 cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta
    16921 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
    16981 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
    17041 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
    17101 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
    17161 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
    17221 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa
    17281 ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag
    17341 ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact
    17401 tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt
    17461 tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt
    17521 tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact
    17581 acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata
    17641 ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca
    17701 ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta
    17761 aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca
    17821 taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg
    17881 tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga
    17941 cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt
    18001 gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc
    18061 acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga
    18121 cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt
    18181 aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt
    18241 tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg
    18301 atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac
    18361 tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta
    18421 aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt tttcagagcc
    18481 atttctacca taatacgttc cacactttct tttttagttt tattaactat ttaaagtata
    18541 aatgaaagcg ttaactaact tcaatgtcat gttgaacaag aattttttct tttttttgtc
    18601 aatgtgctag ttttaagctt taataaatat ccataagaaa taaaaactga tacactggag
    18661 taaataagta tgttaacaat atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct
    18721 catgaccgaa aaaaacctat gatggtgaaa ttagcaacag cgtataaaac aatattggaa
    18781 aaacatgaac tttttgctac cgggacgact gggcagagga tcacggaagc aaccggcttg
    18841 ccgatccatc ggtttaaatc cggaccgtta ggcggggatc agcaaattgg agccatgatt
    18901 tcggaggatc agttagattt ggtgattttt cttcgagacc cgctggctgc acagccccat
    18961 gaaccagatg taacagcact tatccgttta tctgatgtct atgagattcc gttagctacg
    19021 aatatcggaa cagctgaggt gctactaaga ggactggaag ccggcttcct agattttcga
    19081 agaatcgtcc atgaaataga caagcatcca ctttcatttt gatggataag cgtatggaaa
    19141 aataacgttt aatgttcgcc tatatgtagc tataaaagct aaacaaactg ataatatgcg
    19201 atataatacg aacgtattgg ataatataaa caataatatt agtttttcgt atttgcagta
    19261 aatgtgaaaa tatgctatag tttctgtggg aaaagatccc aaaaataata cagagtagaa
    19321 aataaagcgt taagtgctaa gaggatggga tgttgcctct tggacgaagg acgtaatgtt
    19381 cgcggtttta ttttcgcatt cgctgcatga tacgatgtag cagctcttaa aggagatgct
    19441 aaaaatgaag aaacaccggt tagacgcacg tatgattgct attatgggct tattgattgc
    19501 attgatggtc actttgtccc gactggtagc aatcgagacc ccatttatta aaatcagtgt
    19561 cacatttatt ccacaagtga tcatgggcat tctttttggt cctttttggt cagggatagg
    19621 cgcagtttta gctgatttag taggaatggc cctcttctcg aaatctgcat tctttattgg
    19681 atttacattg aatgcattta ttgaaggagc aatctacgga ttctttttct atcgaaaaga
    19741 gatcacttgg aaaaatgcca ttctggctac gcttagtgtc acgctgatca tcaacttatt
    19801 tttgacacca ttatggcttg cgttgatgta ccatgtgcca ttgttcagct gggtcgtttg
    19861 ggcgccccgc ctgttaaaaa cagtaatatg gttaccgatt caatcaattg ccatttatta
    19921 cgtaggacgt agtattcctt acaaaaaaat tttaagaagt ttagcgatcc acgctaaata
    19981 aaaaacaagc agtcttccca tcccagaaga ctgcttgttt ttattgtgct gtgaattgtg
    20041 cttgatagag ccgtcggtaa taaccatctt tttgaagcag ctcatcatgg gtgcccattt
    20101 cgacaatagc tccctgatcc atcactaata tctggtccgc atttttgatt gtggacaggc
    20161 gatgggcaat cacaaaactg gtttttcctt ccatcattgc taagaaggct tgttggattt
    20221 ttttctctgt caatgtatcg actgagctgg tagcttcatc taagatcaac atagggggat
    20281 tactgatcat cgttcgagca atcgtcaaca gttggcgttg tccatctgat atctttaacc
    20341 cttgaccgcc aatcttagta tctaattttt ccggcaaacg ttctacaaat tcatacatat
    20401 aagaggcttt tagggcatca ttgatttctg cgtcacttgc ttccggattc ccaaaacgaa
    20461 gattttctcg caatgtactg tcaaatagcc aagtatcttg taacaccatt ccaaaacttt
    20521 tacgtaatgt atcacgttgg atagagcgga tgtcatggtc atcgactgta attgttcctt
    20581 ttgttgtgtc ataaaaacgc atcagcaaat taacaagtgt ggattttccg gcccctgttt
    20641 ttcctactat agcaatggtt tgacctggat aagcagtaaa attgaaatct tcgatcaaac
    20701 gctgcttggg gtcataagaa aaagaaacat gatcaaaaga gacttttcct ttgatatttt
    20761 tgggttccaa ggcataggca tctggcaaat caggcttttc ttctggctga tcgatcaaat
    20821 caaatgctcg ttctagacca gaaaaagcgg tttgcagttg tgtcgtgatt ccagaaagtt
    20881 cgataaatgg tttagaaaac tgacttgagt agatcgtaaa gctggaaatc acaccaacag
    20941 tgattgacga accactcttc aatgccaata ggccgccaac taaaccaatc gataaataag
    21001 caaggtgatc tacaaaacga gaagacgggt ttgtcaagga agaagaaaat tgtgcccgtt
    21061 gtcctttttc atatagatca gcatttagca gttcaaattg ctgttgacta gcttcttctt
    21121 gctgaaaagc tttcacgatt ttctggttac cgattcgttc ggtcacaaag cccgagatat
    21181 ccccaataat cctttgctgt ctatcgaaat ttgtttgtga agcacgagca acgatccaac
    21241 tcacaaggaa aataattggt gtagaacaaa gaacgaccaa tgtcagaagt ggacttagac
    21301 gcatcataaa aataaacgaa agaatgatga ttgccactcc agaaaataat tgattgaagg
    21361 ccgcagaaat tgcaatggaa atattgtcca tatcatttgt aaatcgactg acgatatttc
    21421 cgtgggagtt ttgatcataa taacgaagtg gcaatcgatt cagatgagca aatgcatctt
    21481 ttcttaattt tgcaacggat aagtaagcta accgattgct taacacttgg atgagccatt
    21541 gactgactac gttgattaaa acgataccac cgaatagaat aagtaaatgg aacagtcgat
    21601 tgaagcggac ctgtccttct ccaatcattt gatcgatact ttgaccaatt tgtaacgtca
    21661 tcacaactgt tgcaatccca ttgatgattc ctagtatcac agctagccaa atgtcttttg
    21721 cataggcagt aaggtaagga atgaatcggc ggagcgtctg caaggataat ttttttactt
    21781 tcataactat tcttcctcct gtgatgcgac gatttcctga tattctgctg attgagcaag
    21841 taattcttca tgggtaccct gagcagcaat cttaccttga tccatcacta agatatgatg
    21901 tgcttcttga atagagcgga cacgttggga gataataatc actgtactgc caagataagt
    21961 ctgtagcgcc ttacgcaaat ccaagtctgt ttgataatcc aacgcactta aagagtcatc
    22021 taaaatcagt aatgctggct tggcaatcaa agctcttgca atcgtcagtc gctgtttttg
    22081 tccgccagag aaatttttcc cattttccat gacttttgta tctaaaccat ttggaagctg
    22141 acggacaaat tcttctgcct gagcgatttt cagagcttcc caacattctt tttctgtggc
    22201 gtctttctta ccccattgaa ggttttcacg aatagtcccg gtaaatagta cagaagtttg
    22261 gggaacaaga gcaatttgct gacggagatg atccagctgc caagaacgaa catccattcc
    22321 gtccattata actgtaccgg aactgacatc atagaaacgc ggaatcaatt gtgtcaaagt
    22381 cgttttcccg cttccggttg gaccaatgat tccaaggact gtatttcgct tcagctgaaa
    22441 tgtaatattt tggagagaaa gaccagaatc aggagtataa cggaagtcaa catggtcaaa
    22501 agcgataatt gcttcagaag aaaaatcggg agtagtactg acctcagctt caagaatcga
    22561 attttctgtg tctagcacct ctttcacccg atttcctgaa gcttcggcac gtgtgaagat
    22621 cacgacgagg tttgaaacaa cgatcagtgc caaaagcatt tgattcatat aattgatcaa
    22681 agcaagaact tgtccttgtt gcaagctacc aatatttact ttgatcccgc caactgtcag
    22741 caaacagatg atcccgacat tcatgatcaa ggttgtagca ggtgataata gggcagagat
    22801 attggaaaca cgtaaataat tgttcgctag atcatttgtg cttttatcga acttctctgt
    22861 ttcttttttt gtctgcgcaa atgcgcggat gacacgaaca ccgctaagat tctgactaac
    22921 gtgttcattg agctgatcta gtttttcttg cactttttgg tagagaggaa cggttttttt
    22981 gatgataaaa aataaaatga ttgagaaaat aggcaataat gctaagaaaa acagacctac
    23041 ttcccagtcg atcacaaaag ccatgaccac tgacccgata cttaaaaacg gtgctctgat
    23101 caataaacga atgaccattg ctaaagctag ctgaagctga ttgatgtcgt ttgtcattcg
    23161 ggtgatcaag gtatctgttc caaaatgatt caactcgttg tgagagaaag tattgatttt
    23221 tttgattagc tgattacgta actctgtacc aaatccttgt gaagctactg aagcatagta
    23281 ttggcaaatg atagcacaaa tcagaccaat cacagacata gcgaccatcc ataagcccat
    23341 ttcaatgatt tttcctcggt cattcatttt tagtccatta tcgatcaagg aagccatcat
    23401 taaaggcagg acaagttcga agacagcttc aagaaatttg aagaacgggc ccaaaatgat
    23461 ttgcttacga tagtcttttg cataacgtaa taactgaatc atatgattcc tcctattatt
    23521 tatttgtcca caattgaaaa aacaagtact accacattgt agcttaggaa atttagaatg
    23581 aaaagagaac aagccttttc ataaatgaaa gttttattat gaagaagctt tagaaattga
    23641 atagactatt ttttttaaaa aaaagaaaat gagaaaacat g
//
LOCUS       DAIGOK010000033        23078 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.033, whole genome shotgun sequence.
ACCESSION   DAIGOK010000033 DAIGOK010000000
VERSION     DAIGOK010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23078
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.033"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..385
                     /locus_tag="NOA01_001041"
     CDS             <1..385
                     /locus_tag="NOA01_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632253.1"
                     /translation="ISEDVESQQFGTNVINKVINPALSINAPLIPTALSFSVTAVVSE
                     IEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLVEEAGEHYA
                     VVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            399..800
                     /locus_tag="NOA01_001042"
     CDS             399..800
                     /locus_tag="NOA01_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632254.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            802..1173
                     /locus_tag="NOA01_001043"
     CDS             802..1173
                     /locus_tag="NOA01_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632255.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1546..1746)
                     /locus_tag="NOA01_001044"
     CDS             complement(1546..1746)
                     /locus_tag="NOA01_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCI0632256.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(2051..3283)
                     /locus_tag="NOA01_001045"
     CDS             complement(2051..3283)
                     /locus_tag="NOA01_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCI0632257.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            3537..4106
                     /locus_tag="NOA01_001046"
     CDS             3537..4106
                     /locus_tag="NOA01_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCI0632258.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(4284..4724)
                     /locus_tag="NOA01_001047"
     CDS             complement(4284..4724)
                     /locus_tag="NOA01_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCI0632259.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            4882..5646
                     /gene="map"
                     /locus_tag="NOA01_001048"
     CDS             4882..5646
                     /gene="map"
                     /locus_tag="NOA01_001048"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCI0632260.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            5678..6601
                     /locus_tag="NOA01_001049"
     CDS             5678..6601
                     /locus_tag="NOA01_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HCI0632261.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            6677..7816
                     /locus_tag="NOA01_001050"
     CDS             6677..7816
                     /locus_tag="NOA01_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HCI0632262.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            7809..8609
                     /locus_tag="NOA01_001051"
     CDS             7809..8609
                     /locus_tag="NOA01_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCI0632263.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            8609..9436
                     /locus_tag="NOA01_001052"
     CDS             8609..9436
                     /locus_tag="NOA01_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCI0632264.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            9414..10148
                     /locus_tag="NOA01_001053"
     CDS             9414..10148
                     /locus_tag="NOA01_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCI0632265.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            10248..11114
                     /gene="rfbA"
                     /locus_tag="NOA01_001054"
     CDS             10248..11114
                     /gene="rfbA"
                     /locus_tag="NOA01_001054"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCI0632266.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            11128..11700
                     /gene="rfbC"
                     /locus_tag="NOA01_001055"
     CDS             11128..11700
                     /gene="rfbC"
                     /locus_tag="NOA01_001055"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCI0632267.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            11722..12750
                     /gene="rfbB"
                     /locus_tag="NOA01_001056"
     CDS             11722..12750
                     /gene="rfbB"
                     /locus_tag="NOA01_001056"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCI0632268.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            12848..13699
                     /gene="rfbD"
                     /locus_tag="NOA01_001057"
     CDS             12848..13699
                     /gene="rfbD"
                     /locus_tag="NOA01_001057"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCI0632269.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            13734..15767
                     /locus_tag="NOA01_001058"
     CDS             13734..15767
                     /locus_tag="NOA01_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632270.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            15811..17091
                     /locus_tag="NOA01_001059"
     CDS             15811..17091
                     /locus_tag="NOA01_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HCI0632271.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            17301..18107
                     /locus_tag="NOA01_001060"
     CDS             17301..18107
                     /locus_tag="NOA01_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI0632272.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            18119..19339
                     /locus_tag="NOA01_001061"
     CDS             18119..19339
                     /locus_tag="NOA01_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0632273.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            19329..20915
                     /locus_tag="NOA01_001062"
     CDS             19329..20915
                     /locus_tag="NOA01_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCI0632274.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            20954..>23078
                     /locus_tag="NOA01_001063"
     CDS             20954..>23078
                     /locus_tag="NOA01_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCI0632275.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALKMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFS"
BASE COUNT         7344 a         3872 c         4764 g         7098 t
ORIGIN      
        1 aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata aagtaattaa
       61 tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt ctgtaacagc
      121 tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg aagttttaaa
      181 caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc ctcctcaagt
      241 taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag caggagagca
      301 ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg atatcaaggt
      361 caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata gtaccatcaa
      421 atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc gcatcggtaa
      481 ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa aatgataatc
      541 aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat ggcaaaattt
      601 ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa tatgatgaag
      661 atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc tcagattacg
      721 atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg aaatctgtag
      781 tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat ttccaaacaa
      841 attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc tgtttcaaga
      901 gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta tgatattatt
      961 tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt ttcaacagtt
     1021 aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga gaaaagaaaa
     1081 tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct gatgaatcga
     1141 gaagcagaaa aaataataaa caaactcacc taatgaattt caagcgagtt tgtttgttat
     1201 ttttttattt aatctttata cctttctgga tcaacgaaag tatactttat atagtcataa
     1261 cgccgatgat cgcttcgtgc gtctggcacg tcagtcacga tatcaaacaa aaagtataca
     1321 tccttcttca ttctagtttt cgcagcagga attttaatat actgaataat agaaaacaag
     1381 tacttgtgcc aaattatgtg ccaaaaaaat tcgaattcaa caaaatgtaa acaaatagaa
     1441 aaccttctat attaagattt cctgcaataa caaacacaac agaagacgtg taatagttag
     1501 tcaggaacgt acaaataacc cctgtatcct ttgcggtaca gggggttatt ttgtataagt
     1561 agacattgta gtttctaaac tgctatgtcc taaacgtttc gatacgctaa gtatatttac
     1621 tccttgataa agtaaaatag atgcatgcgt atgtcttagt ccatcaataa acagtcgaag
     1681 agaaaatagg gaaaaattga ttactaaaga aaaacttact aggtcgacgg tgtagttgat
     1741 tttcatactt caatagattc gaattaaacg atttcgattg gcttatacca gtcctatcca
     1801 acgaaagata tatcgttctt aactctagcg gtataacttg aagctttacg taaagccaat
     1861 gggaatgaac tggagatgac tttttattat ttttagtaat agctgatggg ttaatactta
     1921 caactttcct tttcaaagta ccatataaaa aaacggtggg gttgtttagc gaaataaagg
     1981 atctaagcca atagcttaga tcctttattc tatgctttat attatttttt gcatgtgtgc
     2041 gaaagtatac tcaagcccaa tcgccatttc ggaaaatcgg tactgtagag ccatcttcac
     2101 ggattccgtc gatatccatc ttgtcagatc ctaccatgaa atctacgtgg gtttgacttc
     2161 tattcaatcc agcttctgct aattcttcat cggacatttc tgtaccgcct ttcacactga
     2221 aggcataagc agaacctagt gctaagtgat tcgatgcgtt ttcatcaaat aatgtattga
     2281 aaaagatgat gccagattga gaaataggtg aagggtcagg tacaagtgcg acttctccta
     2341 aacgacgcgc accttcatct gtatcaagca gttttgctaa gacatcttct ccttgttcgg
     2401 cagaaaaatc aacaactttt ccatctttaa aagtaaattt catacccgag atgatggtgc
     2461 cagcataact cagtggcttt gtacttgaaa tgtaaccatc tactcgatga ctgtcaggtg
     2521 ctgtgaacac ttcttccgtc ggcatatttg ccatgaattt ttctccacga gcattatagc
     2581 ttccagcgcc ttcccaaaga tggtttttag gcaatccgat aatgatatct gtaccgggag
     2641 cagtatagtg aagggcagaa aattgttctc gattcaattc ttctgctttt tttgctaatt
     2701 tttcatcatg cttcttccat gctaagacag ggtcttcttc atagacacga gtcgttttga
     2761 aaatctgatc ccataatgcc tcaacttgtt tttcttctgg cagttctggg aagacttttg
     2821 ctgcccattg tttaccagcc gcagccacga cagtccaact gactttattt gcttgagttg
     2881 ccttgcgtaa attcattagt gctttgccat tcgctgattg ataagacgct actcgatggc
     2941 tatctacacc agcaaatgca tcgggatcgg cagaaacaac gctgatcctg cttgctcctt
     3001 tttcaagcca ttcatccgct tgatcgattt tagattgtgg tacattctca atacgatccg
     3061 ttgcagcatg taaaagaaat tctctttgga tctggtcgtc agtccattga acgataacct
     3121 cagcggctcc tagtttataa gcttcttgtg tgatcaatcg agcaaggggt gcctgttcca
     3181 cgctgatctg taaaacaact gtgtgtcctt tttctgtggc tacgccagtt tctgcaatta
     3241 agcgtgcata tttttttaga agttcattaa aatcagataa cataataaaa attcctcctc
     3301 accatttttt ctggtaacta ttagcatgat accatttaat tatgattcaa acaatgaggc
     3361 tttaaataaa aaaaggaggt tattttttta aggttaagtt tcatgatata tgttactact
     3421 tgctatttca gcttgtgaag aaaatcaaaa actaattttg catatcaaaa aaacatgtta
     3481 caatgaaatt aatcaatggt ggaagcgtta taaaaaaatt aaggagatta aaaaatatgg
     3541 caagaaaaaa gacgattact agagaacaga ttttggcagc tgcttatgaa gtagctgcca
     3601 ccgatggatt tacacgattt actgcgagaa acatcgcgaa caaaatgaat tgttccacgc
     3661 aaccgattta tcttgagttt aaaaatatgg atgacttgag acaagctctt tttgagcaga
     3721 tctatgatca tttacaatat gaagtgttca ctgttgagca tacagggaat acgattatcg
     3781 atcttgcttt taattatatc cattttgcaa gaagagagaa gaagttgtat cgttcactgt
     3841 acttagaaga atatggtgga ggaaaagaga tgcagaattt ctcttatact tactttgtga
     3901 atacagtgaa acgcgatcca gcttatgtga atctttcaga agagcagatc gaatctttgt
     3961 ataatgggac atggatcata gctactggga tcgcagcttt gatgtcttca agcatcatcc
     4021 atccaactga tgaacagatt gaaaagatga tccaagacag tatagatgct atcttgaaat
     4081 tcaaagaacc aatcgaaatc gattgaatat actcaaaagt tccacaaatc agtaggtaac
     4141 aagagatgtg tcaaaataag gaaaagttcg tttttcttac agcgtatgca tttttaactc
     4201 ccgaagaact cttccatgaa tgcaatgaat aaaagagcca tacagattga ataaatctgg
     4261 atggctcttt tattgcaagt atattaaatt tttgcgacta gttctttagc gaaagcttct
     4321 aaattttcga tatcttcttc ttcagcattc agatccactt taacattttc ggctcctttt
     4381 gatgcgcctg tttttgcaaa agcagcatcg aaatcatcca ccgatttgca gaattcatcg
     4441 taaaaagtat caccagatcc gcagacaccg taaatttttc ccaatagatc caattcttgc
     4501 aagtcttcat aaaagtctac gatctcatca ggcagatcgc catcatcgta tgtataagta
     4561 gctactacgc aaatatctgc ctcctcgaat tcatctgcat ctacttgggt acattcgttg
     4621 atttcgactt cgatatccag attttcaaat gcttctgcaa caatatcggc gatttcttca
     4681 gtgtttcctg tcatgctagc atatacgatt tttgctaaag ccatgatgtg cctcctcgtt
     4741 tataattatt taacaattat gaattatacc aaaacatcat tgaaaagaaa ataaaaccta
     4801 aagacacctg gggaatgtca tttggctaaa tgtatgccgc tatgttaaaa tgagagggat
     4861 ttgtaaaaag gagacgaaaa tatgattact ttaaaatcag cacgtgaaat tgaaatgatg
     4921 gcagaatctg gagctttatt agcagatgta cataaaaatc tgcgtgattt tataaaacca
     4981 ggaatcacaa gttgggatat tgaggtattt gtcagaaact atatcgagag tcatggcgga
     5041 attgctgcgc aaattggctt tgaagggtat gaatatgcga cttgtatcag tataaatgat
     5101 gaaatttgtc atggattccc tcgtaaaaag cctttgaaaa acggtgattt ggtaaaagtc
     5161 gatatgtgta tcgatttgaa aggcggcatt tctgattctt gttgggccta tgtagttgga
     5221 gaatctacac ctgagatcga ccgattgatg gaagtaacga agaaagcttt gtacatcggt
     5281 attgagcaag cacaagtagg gaatcggatt ggtgacatcg gtcatgctat ccaaacttat
     5341 gtagaaggcg aaaatctagc gatcgttcgt gattttattg gtcatggcgt tggtcctacg
     5401 attcatgaag aaccagcagt tcctcattac ggagaagctg gaaaagggtt gcggttgaaa
     5461 gaaggcatgg ttatcacgat cgaacctatg gttaataccg gaacttggaa aatgaaaatg
     5521 gatcctaacg gctggactgc gtatacaaga gacggcggac tttcttgcca atatgaacat
     5581 acattggcta ttactaaaga tggtccaaga attttgacat cacaaggaga agaaggcact
     5641 tattgatcta tccagtcaat aggtacggga gaggtacatg aaagtttgga acaaaataaa
     5701 acaaaatgaa aatctgatgc gctttattga gacgactcaa aagcgcatca ctgattcaga
     5761 aatgagtacg acctctgtcg tcgttgctta ttatttactc ttgtctttat ttccattgat
     5821 tattgctttt ggtaatattt taccgtttct ccacatcgat caagagactg tactgaccta
     5881 tttacgagaa gtgattcctg agaccgttta ccagtttatc ggtcctgcaa tcaaagacct
     5941 tttgacacaa agttcaggag gcttgttatc cgtttctgca ttagcggctt tatggtctgc
     6001 aagtcaaagt atcaatgctt tgcaaattgc gatgaataaa gcgtatggcg tagaaaatag
     6061 aaaaaatttt ttgatcgtcc gtttcttctc actaattgtc attgtcttgt tcatgattgc
     6121 tattagtggg gtaactatcg ttcttgggtt gggacagctg attttggatg ctattcagcc
     6181 aatcgtacaa atacccgtga actttatcga tcagttccaa actttgaagt ggccaatcac
     6241 attggtagct ttatttgtca tcatgttctt gatttattta attgtcccta atgctcagtt
     6301 gaagttgaag acgatcattc cgggcacgat cttcgctact gtcggctgga tgctgttatc
     6361 acaagtgttt agtatctatg ctaggtattt tgcaacgagg atcagcggtt atcagatcat
     6421 aggaagtttt attgtactca tgctttggct taattttgct gctacgatca tcattttggg
     6481 cggaatcatc aatgcagttg ttcaagaata cgtgacgggt catcaaatca aaaaacgtcg
     6541 agatccaagc cgaaaatggt tcaataaatt gaaaactaaa ttttctcata aaaaaaagta
     6601 gaaacaagag ttcttattgt aaatttatat cagtaattta taataattat gactcttaat
     6661 taaagtaggt gcccatatgc ttgtttcttt tagtatggtt ataagatttt ttctgacaat
     6721 tttattatca ctgatattaa cacctgtatt caaaatcatt tcagtacaaa caggtatggt
     6781 cgacaaacca aatgaaagaa gaataaataa agttccaatg ccttcagcgg gcggactgcc
     6841 tatctttatt tcatttgtta tatcgacttt attcttattc cgtaaaatta tccctcaatc
     6901 gtatattgta ccgatcttga ttgcttcttt agtaatcata gtcacaggag tgctcgatga
     6961 taaatatgag ctgacaccta aacaaaaaag tatagggatc ttgattgctt ctcttattat
     7021 ttattttgta gcagatatac gtattgattc cttcacgctt ccttttattg gatatatcca
     7081 actaggctgg cttagttttc ccgtaacgat tttttggatc ttcggtatca cgaatgcggt
     7141 caatctgatt gacggtttgg atgggctggc ggcagggatt tcattgatcg gtttaattac
     7201 gataggaatc attggatatt ttttcctcca tgcttctaca gtgtatattc cagttgttat
     7261 tttctgtctt gtagcaagta ttatcggttt ttttccttac aatttttatc cggcaaagat
     7321 ttatttaggc gatacaggag ctctattcct agggtttatg atggccgtcc tttcactaca
     7381 agggctgaag aacgtgactt ttgtttcatc gatttctttg ctgatcgtca tgggagtgcc
     7441 ggtaacagac acgttttttg ctatcattag gagaaaagca aaccgtgttt ctttttctac
     7501 agcagataaa aaacatcttc atcaccgact aatcagttta ggctttactc ataaaggtgc
     7561 tgtcctgacg atttatgcga tggctttgat gttttctttc acagctatgg tcatgaatta
     7621 tactggaaga atcggaacga tcatattgat tattgccatg ttgtttgctg caattttatt
     7681 atttgaattg attggtctga tcaatgagaa acatcagttt attttaaaaa cattgcgatt
     7741 tttagggaat aaagagtacc gtacgcaagt aaaacagaaa tatttgaaga aatggaaccg
     7801 tcatatagat gaataacaaa ttaaaccagc cgatccttat tagtattgtg acggcaaaca
     7861 gtgataaaat ttttcggaca ctagataatt ttatttcaac tgtaaaagat gaaacagcag
     7921 taaaaatccg aatttttgac aacaattcac ttcccgagta caaaaaaaga ctaacagagt
     7981 ataggaaata tccctacatc gatatctatt ttcatgatga aaattccgga tttggttatg
     8041 gacataatca caatctgata gctagcgatt ctcgctatgg cattatttgt aacccagata
     8101 ttttagtgga tgaagagaca gttacagcat tggtcttgtt attgaaagaa catccggaat
     8161 gtgcgatgtt agcacctaag attcttaatg aagatggaac aactcagcac ctcatccgtg
     8221 agcagctggc tgtttttgat tacgttttgc gctttgtgcc tttcaaatgg gtcaaaaaaa
     8281 agtttgacaa acgactggct gtgtttgaat gccgaggatt gcctactgat gctaatagtt
     8341 atgtacggat gatctcaggt agttttatgg tagctgatat tgataaattt aaagagataa
     8401 aggggtttga tgagcgtttc ttcatgtact ttgaagacaa cgacttgtgt ttgagttttg
     8461 aaaaagcagg ttacaagtta ttgtacacac ccttttatgc agttatccat ttttacggga
     8521 agggtgcgca tcgaaatgca aaactgtttc gcgtgttttt gacttctatg atgaaatttt
     8581 tcaataagtg ggggtggcgt tttttctgat gaaaccagaa atattagtag tcgttgtgct
     8641 ttatcagcaa ctattcagtc aatctccagc ttatggacca ttaagcaaag cacttagcga
     8701 aaagcaaatt cagcttatta tttacgataa tagtcctcaa aggcagcaaa atgagctgtt
     8761 tgaaaaagaa aatatcgttt atcatcatga tccgcaaaat cctggtttag cggtggctta
     8821 taattttgcg ttgtctcaag cagatgaagg aacacgttgt ctcgtcactc tggatcagga
     8881 tacgaggcta gtcgacgatt attttgaact attgcgtaaa gtaaccttta cagatgaatg
     8941 tgttgctgca gttccgatga ttttttctgg gagccgacaa gtttctcccc tatattcaga
     9001 tcgttatatc aatcgtacag ctcaagcagt agaagttgaa ggcccaacag ctgaacgaat
     9061 catggcaatc aattcaggtg ttgcctggtc tgtcaaattt ctaaaaaaga ttggcgggtt
     9121 taatcctgct ttttctttag attttttgga tcattggctg ttttggaaag tggatcagtt
     9181 ggaaaaaact gtggagatcc ttcctgtacg aatggaacat gatctttcgg ttttagacta
     9241 tgaaaaagtt tcaagtaatc gataccgttc tattttacaa gcagaaaatc gattttatca
     9301 tgaatatgat caaaggcatt tatccagtca ccgacgacaa ttattgttaa gaacagctaa
     9361 acagttcttg accgtcaaaa accggcatat ctggcggatg actttgcgct catatgttaa
     9421 gaattggaag gtgtgaattc gtgcgttcgg tttgtatagc tacctttaat ggaggcagat
     9481 atttaagaga gcagttagat agtattttat ctcagctttc agcagaagat gaagtagtca
     9541 tttctgatga tgggtcagta gatgatacat gggatatatt aactgaatac gcagaaaaag
     9601 ataagcgaat ccagcttttt tcggggcctc gtcaaggatt gatcgctaat tttggttatg
     9661 cgatccagca gacgaaagga gatctaatct ttttggcaga ccaagatgat gtttggttgg
     9721 agacaaaagt gaaaaagatc actgactatt ttgagcagaa tccggatcag ctagtagtag
     9781 tttctgattt aatcattgtg gacagcgagc tgcaagagat ccatgattcg tatttttcgt
     9841 ttcgaaattc caaagaagga tggcttaata atgtgataag aagtcactat attggtgctg
     9901 gtatggcttt ccggtcttct ttaaaacccg atattttgcc gatcccgtca gaagtgccga
     9961 tgcatgatat gtggattggt ttgctgggag gaaagaagac tgggttccta agagaaccgt
    10021 taactctgta ccgaagacat gatttaaacg cgagtgagat tgaaacacat tctaaattac
    10081 ggcaaaaaat ttcttggcgt gttcatctga ttacggcgtt gatcaaacga aaatggttta
    10141 aacgttgata aaagatgttt aaatccttaa tatttccatg acaaatataa caattgatga
    10201 gataataaca aagtgaaatt tgaaatcaaa aaggagagca gatatccatg aagggaatca
    10261 ttcttgctgg gggcagcgga acacgtctgt accctttaac aaaggcaaca tcgaaacaat
    10321 tgatgccgat ttatgacaaa ccaatgattt attatccaat gtctactctg atgttggcgg
    10381 gaatcaaaga aattctgatt atttcaacgc cacaagatac accacgtttc aaggaattat
    10441 ttggaaacgg aaatgattta ggactgcaga tcgaatatgc agtacaagaa agtccagatg
    10501 gattggcaca agcgtttatc attggagaag aatttattgg agatgatagt gtctgcttgg
    10561 tattagggga caatatttac tatggtggcg gtctgtctaa aatgttgcaa cgagcagctt
    10621 caaaagatac aggcgcgaca gtatttggct atcatgtcaa tgatccagaa cgctttggtg
    10681 tagtagaatt tgatgagaat atgcaagctt tgtcgattga agaaaagcct gctcagccaa
    10741 aatcaaatta cgctgtaact ggcctttatt tttatgacaa tgaggtaatt tctatcgcta
    10801 aaggtatcaa accttcagaa cgtggagaac tagagattac ggatgtcaat aaagcatatt
    10861 tggataaagg aaaactatcc gtagaactta tgggacgcgg atttgcttgg cttgatacag
    10921 gaacacatga atcattgctt gaagcatcta cctttatcga aacaattgaa aaacgtcaga
    10981 acttaaaagt ggcttgtttg gaagaaattg cttaccgtat gggctacatc gataaggaac
    11041 aattgctggc ccttgcacag ccattgaaaa aaaatcaata cggtcaatat ttattgaatt
    11101 tggccgctga ataaggagtc cttacagatg aaagtaacag atacaaaatt aacagatgtc
    11161 aaaatcgtag aaatggacgt ctttggcgat catcgcggtt tcttcacaga aagttattca
    11221 aaagcaaaat ttgcagaaca tggattgaaa tatgactttg ttcaagacaa ccattcacta
    11281 tcagctgaag ctggtgtcct tcgggggctt catttccaaa aaggagaagc tgcacagaca
    11341 aaattgattc gggtagtcac tggagcagtt ttagatgtag tagtggatat ccgcaaaggt
    11401 agcccgactt atggtcaatg ggacggattt atcttatctg aacataatca tcgacagttg
    11461 cttgtaccta aaggatatgc ccatggattc gttactttga caccaaatgt gaacttcctt
    11521 tataagtgtg ataattacta taatgcagaa gcagatgggg gcatcgcatt tgatgatcca
    11581 gatttagcga ttgattggcc aattgatcca tcaagagcga ttatgtcgga aaaagatcac
    11641 cgtcatccga cattaaaaga atttgaagca gaaaatccat ttgtttacgg agaaatctaa
    11701 gaattaagga gaaattttct tatgaaaaac atcattgtta ccggaggtgc cggattcatc
    11761 ggctctaatt tcgttcatta cgtggtaaac aatcatccag aagtgcatgt cactgttttg
    11821 gataaactga cttatgcagg aaacaaagag aacttggccg gacttcctag cgatcgcgta
    11881 gaattggtcg taggagatgt tgcagatgca gaattagtgg accgtctagt aaaagaagcg
    11941 gacgcagttg ttcattacgc ggcagaatcc cataacgata attcattaaa agatcctttc
    12001 ccatttgtac aaacgaatat cattggtacg tatacacttt tagaagcttg ccgcaagtat
    12061 gatgtacgct atcatcacgt atctactgac gaggtttacg gcgatcttcc attacgtgaa
    12121 gacctgccag gacatgggga aggcgaagga gaaaaattca ctgcggaaac accatataat
    12181 ccttcaagtc catattcttc tacaaaagct ggttcagatc tgttagtaaa agcatgggtg
    12241 cgttcttttg gcttacgtgc aaccatttca aactgttcaa acaactatgg tccttatcag
    12301 catattgaaa aatttattcc tcgccaagta acgagtatct taagcggtat tcgtccaaaa
    12361 ttatatggcg aaggaaagaa tgttcgtgac tggatccata cgaatgatca ttcttcagca
    12421 gtgtggctga ttttgacgaa aggcagaatt ggcgaaacct atctgatcgg tgcagacggt
    12481 gaagaagata ataagaccgt gatggaattg atcttagaaa tgatgggcca accaaaagac
    12541 gcatacgacc atgtaaacga ccgcgcaggg cacgatttgc gttatgcaat cgattcgaca
    12601 aaattgcgag aagagctagg ttggcagcct gagtttacga acttccgtga tgggttggct
    12661 gacacgatca aatggtacga agaacatgaa gactggtgga aaaaagaaaa agaggccgtt
    12721 gaagcagcat atgccaaaaa tggacaataa tacagaggaa atttcactgt attaatggaa
    12781 aaacacatgt gaggcaggcg ctcgcctgtc tcttttttaa aataaaaatc aaggagcgga
    12841 taaccatatg tttttattaa ctggaggaaa tggacaatta ggtacagaat tgcgccattt
    12901 attagatgaa aaaaatctgc catatgtttc aacagatgca aaagacttgg atatcacaga
    12961 tgctgagaaa acaatggctt atattacaga attaaaaccc gatgttattt tccattgcgc
    13021 agcttacaca gcagtagata aagcagaaga agaagcaaaa gaattagatg agaaaatcaa
    13081 tgtcgatggc acaagaaatg tagcacttgc agcaaaagct gtgaatgcac aattcgtcta
    13141 tatcagtaca gactatgttt tcgatggaaa aaacaaaaat gtagaatacg aagtagacga
    13201 ccaaacgaat ccactaaatg aatatggtcg tacgaaatta ttaggcgaaa aagcagtaga
    13261 agagatttta gaagactatt atatcatacg aacttcttgg gttttcggga tctatggaca
    13321 caatttcgtc ttcacgatgc aacgcctagc ggaaacccgc gacaaactga ctgtagtaaa
    13381 tgaccagttt gggcgaccaa cttggacacg tactttggca gaatttatgg tatatgtcat
    13441 cgaagaaaaa gcaccatttg gtatttatca tttgtcgaac gaaaatagct gttcttggta
    13501 tgagttcgca aaagaaatat tgaaagatac agatgtagaa gttgcacctg ttacgtcaga
    13561 agaatttccg caaaaagcaa cacgtccgca gtattcagtc atgagcctga aaaagacaga
    13621 agcgttaggt tttgtcatac caacttggca agaagcactt gcgcaaatgc tggaagctgc
    13681 caaaaaaatg aataagtaat ttccttttta tcaaatagaa tttggagcat gatatggaaa
    13741 aacaaacaag aagtgcacga cgaaaagaaa agccaaaagc gaatcaggca gattcgaatt
    13801 tggtgcttga cctgtttcta aaactccgct atcttattgg cgttttagtc atcgtggtca
    13861 tcgttacttt taatttaaac ggaagttcac ttggtgtttg ggataaatat gtgtcgcaaa
    13921 gagacgatgg gaaaaaaact gatgtgattt ttggacagaa tcgggaagtg cggtcagacg
    13981 aatggctggt gcagacacct ttttacctct cacaagctga agaggacttt tcgttggtga
    14041 acgaggatta ttcattaaac ggacaaaaca tgatcattgc gtataattct cctgtaaagg
    14101 atattacggt tatcggaaaa ccattcaact ggggattctt tttcctcgga agagatcgag
    14161 gactttcctt ttattgggcg atgaaattag tcgtcatggt cttgcttgga ttcgaagtac
    14221 tgatgatatt aacgaagagg aaaaaagctc tatcgctaat cggtgcattt gcgctgacat
    14281 tttctccagc agtccagtgg tggttcatgc aacatgtggg cgatttgatt ttctttactt
    14341 tgggactgat gattgctttt tatcattatt tctacaatca tgataaaaaa tggctacgaa
    14401 cgttgatgat gcttttggtg gttattttcg gtattgggtt tattttagtg atttacccag
    14461 cccatcaagt gatgctggcg tacttgctga ttttttattt tgttggcctg cttatttact
    14521 atagcaagaa agtttcatgg gatctgttgg atgtagtgtt gattggaggg gcagttttat
    14581 ttattggcgg agtcatggtt catttctggc taacgtcaaa agacgcattg ttagcttctt
    14641 tgaatacctt atatccagga aaacgggtaa gtactggagg aaagtggacg attggcgaat
    14701 ttttttcttt ccttacaaat tggaaaattc cttttgaaga tatcaactat tcgaataact
    14761 ctgaagtggc cctgttctat cacttttttc caactgtatt cctagcaagt ccatttgtgt
    14821 tatttggcaa aaaagatagc gaacaaaagt tactgggacg tattttgatg attttttgtc
    14881 tgttttctat cttttggatt accgttgggt tgccaaaatg gctagcagaa atcactctgc
    14941 tgtcttatgt tcctccatta agagccatcc ttacttttag ttttgcggct tgtttattat
    15001 cgatctggtt tatcgcttat atttggagag aagatgttat tcctatttgg tataaattca
    15061 ttttgttggc agtaaatgca tggtcttatt tctatgcgat tacgcattcg aaaatggata
    15121 attatttttc ggattttgaa accatcgcag ttgctgtgtt gggaacagtg attttagctt
    15181 ttgcattatt tggctggaga cgcttgtttt acctcgcttt tgtcgcctta gttatcgtat
    15241 ctggcttttt cgtcaatcca gtcgtagaag gtacaggagc gatttttgaa aaaacactgg
    15301 caaaagaaat ccaaaaaatc gatcaacaag atcctggtaa agtttggttg tctgaagatg
    15361 aattatataa cttcacccca acattgggag tgaaatcctt caatacagta cgcttttatc
    15421 cagatatagc tgcatggaag aaaatcgatc cgaaagaaga atacgagaag tattataatc
    15481 ggtatgctca tacaagagct tttatcgcta aggaagatac aaagttcatt ttagatcgtc
    15541 cagatatgtt ttcggtcacg ttgaattttg aagacgcaga aaaattaggg atcgactatg
    15601 tgatcagtaa acgaccatta actgattaca accaattata tgaagcgcag ttcacgcagc
    15661 tatatggacc ggataaagac ggttatatga tttttaaagt agaatatccg gattatccag
    15721 atgtaccaca aacacaaact caggaaaatc agctagtcgt acagtaatag gctagctgag
    15781 aataatcctg aaatattagt agaaaggaga gtgaagataa ttcaagcaat cgtacaaaaa
    15841 tttgtcaaca aaatgagtgt cggtttcttg cttttgttgc tgacgctagt cttttttgtt
    15901 tggacagcag gggtaggttt tccagcaact ttgtttatat ataacaatag ttcattactt
    15961 atcgctttta gtgtggctat tttgctcatt ttaaatgttt ataaagcaaa aggcgtcgat
    16021 tttctttatc taggtgttgc gcttgccctt ttcattttct tttcttactt tcgggaatta
    16081 agaaatgagt ctttatttgg agacttactg tttcctttga ttattttatt ttgtttagta
    16141 attaaatgga tcgagtggac aaaatgggat cgcggtattt atctgattgt ctttacggtc
    16201 ttcctcggaa tcacgttata tcgtgtattt acagagatcc aagtaccaga aggacaaagc
    16261 atctgggctc caggcaattc gctttctgat atttggatca atacgaatac aatcggaagt
    16321 tctttgatga ctcttgcttt attgatcagt ggatttgcca gttcttttga acgatggtat
    16381 gttcgaatca tcggtgggat agcagtcgtt gcagcctttt taggtatttg ggtctgccaa
    16441 tctagaggtg cattagtcgc gttgattgtt tttgcgatat tggatctttt acctaaatcg
    16501 ttgatgcgtg tcattcgtgc gccatttatt gcctacacgg ttacgattct tttagctttg
    16561 ccgatttctt atctggcggc tgtctctgaa aaagtcaatt tgtttaccgg tcgagaagat
    16621 atttggcata aattctatca gacgctagga gaaaaatctg aacaagtttt actaggaatg
    16681 aaaacattta tcttccagcg aggaaaccaa tttttaggaa accacaatag ttataattct
    16741 attttgaata tctatggatt gataggattt gggattgctg ctttgctgct tatccttttt
    16801 attggtcgct taacgttgaa agcagattta tccaacgggc agatgacttt tatctgggcc
    16861 ttttttgcgg tgatgatgca gtcatttatg gaggatacat tgacttcata cccatgggtt
    16921 ccgattattt atatcctgtt ggctatggcc gttcatcgat atgatgaacc agaagttacg
    16981 gaggaattct atgttatgga agaggcatat gacgatatga cttatgatga tacatcttat
    17041 gaggaacaac cagatagcgc tcattcttcc aggatccaac gctatcattg ataggcacaa
    17101 aatacgagag aggctgtgac aaccgtcgca gccttttttg gtttattgca attgtctagc
    17161 atcttctgta aaatagaaac gttagagaaa atcttgtatc atagaaaatt aagaaagaaa
    17221 taagaacgca gctcggtacc aaaaatatgg taaaatggca cttgactgta attcactgaa
    17281 aatcgcttgg aggaacacaa atgtttaaag atttgtattt atttatgaag gacatttatc
    17341 agaacagaaa attactgcta cagttttcga tcaatgactt caaatcaaga tatgcaggat
    17401 cgtttttagg aatcttatgg gcgtttatca atccgctatt tactgtattg atttattggc
    17461 tggtatttgg tgttggactg aaagcacaaa tgatcgatgc aaaataccca tttatcgtat
    17521 atttagttac cgggatgatt ccctggttct tcttctcaga tgcatttgca tcaactacgg
    17581 ttgtttttcg ggaatatact tatttagtaa agaaagtcgt attcaatatt cgtatcttgc
    17641 ccacgacgaa aatcctatcc aatctgtaca cccatttatt cttcattctg attggcatag
    17701 ctgtcgcgct tataaatggt atatatcctt cagcaatgtc tttacagctg atttattatt
    17761 tattttgcat gtgtgctttc ttgacaggat tgacttggct gacggcatcg atccaaccat
    17821 ttttacctga tatcatgcaa ttaattacga tcgtgctgca gttgatcatg tggacattac
    17881 cgatattatg gagccctaat cagtttgatt caggtcttgt aagcatattg aaattaaatc
    17941 cgctgtacta tgttgttcaa ggttatcgtg agtcattttt aagtgaagct tggttctggg
    18001 agcatggaac atatactttg tatttctggg ttttcacgtt agtaatgttc gtaatcggct
    18061 ctgcagtgtt ccgtagattg aaaccacatt tttcagatgt gctgtaagga gaatagctat
    18121 gacagaatat gcaattagac taaaagatat cacaaaatct tataatatgt acgcaaaacc
    18181 ttcggatcga tttcgtgaag ccctcaatcc ttttaagaaa tcctatcatg atgtatttta
    18241 tgcgttgaaa aacgtgaatg tagaaataaa aaaaggtgag atgatcggtt tcgtgggaga
    18301 aaacggatcg ggaaaatcta caatgttgaa aatcattacg ggagtactga caccaacatc
    18361 cggcacgatg gaaatcgaag gaaaaatctc agctttgcta gaactaggat caggattcaa
    18421 tcctgagtat tctggctatg aaaatatcta tttaaatgga atggttctag gattttcaag
    18481 agaagaagtc gatgaaatgg tcgatgatat cattgaattt gctgacatcg gcgaccatat
    18541 ttatcaacct gtaaaaacct attcaagtgg gatgttcgtt cgtctagctt ttgctgtagc
    18601 gatcaatgtg aatcctgata ttttgattgt ggatgaagca ttggcagtag gagatcttga
    18661 atttcaacta aaatgtatgg aaaaatttac agaaatcaag aactcgggaa agacgatttt
    18721 attcgtttct catgatatta gttcgattcg ccgtttttgt gatcgttcct attggttgaa
    18781 aaatggaaaa gttgtcgaat atggtgatac gatggaagta acgacgaatt acgaaaactt
    18841 tttgaagaaa aaatcagtta aaacagtaga tcgaaataaa aaccaagtag aacgcttcgc
    18901 agctccagat atcgtagacg tgacgaaagc cgaattgttg gataaagata aaaatccagt
    18961 ggatatgctg gaacaaggag aaaaactata cgttaaagta acctatgaag taaaagacga
    19021 tacaatcaaa aatccagttt taggaattgc tctgcgtaca gtcgacaatt tttatgtgtg
    19081 cggactcaat actctactag atggtatcaa gatcccttgg aaaaaaggtg agaatatctt
    19141 ttatctggag tatgaaaagt taggtttgtt agctggtgaa tattattttg acgttgcggt
    19201 ctttgaagaa aatgcaacgg ttcctttggt atacaagaca aaatacatga acttgtttgt
    19261 cagcggttct tatatcggag aaggcatcgt ggtcttagac cataaatggg aagaagggac
    19321 tcatagcaat gaaatatgat tttgaaatgg acctagatga acaaagcagt gtgggaaaaa
    19381 ttgccgcaca gatcaaacca ggaagtaaag ttttagagtt tggtcctgga aacggccgat
    19441 taacgaaaca tttgatcgga gcaaaacaat gtgaagtgag cattgtcgag ctagataagg
    19501 aattgttcga ttttgtcagc gagtttgccc aagatggatt ttatggagat atcgaaagtt
    19561 ttgaatgggc aaactattat gctggacaaa catttgatta cgttttgttt gccgatgttt
    19621 tagaacattt ggtagatcct ggaaaaacgt tgaagaaagt tagagaattt ttgaatgaag
    19681 aaggagaaat tctaattaca ttccctaacc ttgcgcataa ttctgtcatg attgatttgt
    19741 tcaataatca attgccatgg gcttcttatg gtttgctaga tgaaacccat aattcttttt
    19801 atactcatga tggcttccaa aaagtttttg aaaaagcagg attatacatc aatattgaag
    19861 attatttgta cttagctgta ggtgatacag aattaaaatc aacttatgaa gaattgccgg
    19921 aagctgtacg ttatgatttc aaaatgcgac catttggcga agtctaccaa tacttcttct
    19981 cattgatgaa acatccagta gcacaaagtt ccattgcgca gccgcaaaat tctaattatg
    20041 tgaaagtatt agaagtaatc cagcaaacga aacaagatga aataattcaa caaataccat
    20101 tcaataattt cacaggggaa aatgagacac taagtttcca tgtatcagaa ttagcagaac
    20161 gaatggtgtt tcgcttcgcg cagcaaccta gctttatcga attcagcgca gaagctgctg
    20221 gagaaaaact cacatttatc gattcaaatg ctgtagtcaa aacagtaaat gattgttatt
    20281 tgtttgatgg taaagattta ccagagttct ttttaacgga tattgcaggg aaagaagtga
    20341 ctatccattg tcactatcgt tttatcggag aattgacacc aacaatgaaa gaattgctgg
    20401 aaacaatccg tccaatggct gaagttacaa agcagctttc tgagaaaaat gatgaattag
    20461 aaagagaaaa tcattatctt attgagaata atacaagatt agatcagacg ttgaaaacaa
    20521 ccataaatcg ctattgcacg ttgttggaaa gtgatgaatg gacaatcaaa catagattag
    20581 ggcgacgtaa gaaagaaaca tcgaaaaaaa tccaagaaaa agagctttct ctatgtattg
    20641 acgaaaaaat ctgggatgca gaaacaaaga tattgaaaat catcgggtgg ggaatcgcta
    20701 attcggatcg ccaaccattg tcctataagt tatcagccga ccaatcacca ttttttgaag
    20761 caattcctgt tttacgagaa gaagtcaatc aagcaaaaca gctagctgaa ggagcaaaag
    20821 caggatttga actgcggatc ctttgcgaga aggaacgttc attcttagtt gaagcagtgg
    20881 ctcaaaatgg tcaatcatgg atcattgaaa tgtaaataat gattaactta taaagataag
    20941 gagtaaacga atcgtgcaaa aaaaagaaat tgccgtattt atcgatcaaa tcactcgtga
    21001 acgggccact ggcgatttgc ttattgtggg ctgggcaatt gatgaagtga ccaaagaaat
    21061 ccctacgata aaggtagaaa aagaaaatgt gattgctgaa gcaacacatg tcgtaagatt
    21121 ggatatcaat catttatata atcttgatgt taagacccaa agcggtttta aggtacgttt
    21181 atcagggaaa atgagaggaa aagcaatctt agacttccaa acagcaagac atcaaaatgg
    21241 catagctgtg aaattaaacg gtaaatatcc ttatgatgac ggcattgaat cttcatggga
    21301 aagaaaaaaa agattactga aaaaagggat aaattatgca aggactcatg gcgtaaaaaa
    21361 agcaatccgc cgggtcaaac tggaattgaa accaggatcg atcgactatg cagagtggat
    21421 cagtcgtcat gagaaacctg atctgaaaga gcaaaggaaa gaagtacaag gatttgccta
    21481 ccgcccattg atttccatac ttatgcctgt ctataacgtt gaaatcaaat ggctcgaaaa
    21541 atgcattgat tcggtacttg atcaaacgta tgatcactgg gaactatgta tcagtgatga
    21601 tgcttctact gatccagcta tccgaaagtg tctagaatcc tatcaagcaa aagatgatag
    21661 aattaaagtc gttttccgac aagaaaatgg acacatcagt ctggcaacta attctgcatt
    21721 gaaaatggca gaaggagagt ttatagcgct cttagataat gatgacgaat tacctccatt
    21781 tgccttatat gaagtggcaa aagtgctaaa cgtacatcca gaactggatt tgatctacag
    21841 tgacgaagat aaaatcgatg cagatgggaa tcgctttgac ccgcacttca aagcagactg
    21901 gtctcccgat acattgatgg gaaataatta tatctctcac ttaggcgtat atcggacaag
    21961 cattgttaag gaacttggcg gtttccgtaa aggatatgaa ggctctcaag attatgattt
    22021 ggttcttcgt gtgacagaac aaattcctgc agaacacatt taccacattg accgtgtctt
    22081 gtatcattgg cggacgatcc cgggatcaac tgcaagcaat ggagaagcta aaagctacat
    22141 ctatgattct ggagtgaaag cattgacaga tgctctatct agaagaaata tcaagggaag
    22201 cgtacatcct ggcagaattt ctggttttta tgaaatcact tatgatgttt tgcaagaaga
    22261 cttggtcagt gtgatcattc caactaagaa tggctatgaa gatctaaaaa cttgtgtgga
    22321 ttcgatcatc gaaaaaacgt cttatccaaa ttatgaaatc attattgcag ataatggaag
    22381 tacagatcct aaaatgcaag aattatttgc agaatacaag catcagctga aagaccgatt
    22441 tatcgtcgaa ttaattgaca ttccattcaa ttattcacgt atcaacaatc tggcagcaga
    22501 aaaagcaaac ggcaaatact ttttattctt gaataacgat acagaagtga ttgaacctga
    22561 ctggatgaca gcgatggttt cttatgcaca gtttgatcga atcggctgtg taggagcaaa
    22621 attattttat ccggatgata caacgcagca tgcaggtgta ttattaggga tcggtggcgt
    22681 tgcaggacat gcattgaata actatgatcg aacacattgt ggttactttg gccgattggt
    22741 cattgacgtc aattacttag cagtcactgc agcttgtatg atggtcaagg cagctgactt
    22801 caatgctgtc aatggatttg atgaaatatt agaagtagca ttcaacgatg ttgacttgtg
    22861 cttgaaagtt tatgaactgg gtcgttacaa tgtctatgca catcaagcag aattgtacca
    22921 tttcgaatct aaatcaagag gttatgaaga tacaccagaa aaacaaagac gttttgctgg
    22981 ggaaatcaaa aaaatgcaag ataagtggcc ttcttatatt gctcatgacc cattttataa
    23041 cgataatcta actaaagaag gaatcggcga tttttcgt
//
LOCUS       DAIGOK010000034        22800 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.034, whole genome shotgun sequence.
ACCESSION   DAIGOK010000034 DAIGOK010000000
VERSION     DAIGOK010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.034"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            84..1601
                     /locus_tag="NOA01_001064"
     CDS             84..1601
                     /locus_tag="NOA01_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HCI0632276.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            1573..2862
                     /locus_tag="NOA01_001065"
     CDS             1573..2862
                     /locus_tag="NOA01_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCI0632277.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            3037..3933
                     /locus_tag="NOA01_001066"
     CDS             3037..3933
                     /locus_tag="NOA01_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCI0632278.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            3947..4810
                     /locus_tag="NOA01_001067"
     CDS             3947..4810
                     /locus_tag="NOA01_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCI0632279.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            4816..5379
                     /locus_tag="NOA01_001068"
     CDS             4816..5379
                     /locus_tag="NOA01_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HCI0632280.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            5401..6912
                     /locus_tag="NOA01_001069"
     CDS             5401..6912
                     /locus_tag="NOA01_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HCI0632281.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            6924..7883
                     /locus_tag="NOA01_001070"
     CDS             6924..7883
                     /locus_tag="NOA01_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HCI0632282.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            7969..9048
                     /locus_tag="NOA01_001071"
     CDS             7969..9048
                     /locus_tag="NOA01_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCI0632283.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(9300..10700)
                     /locus_tag="NOA01_001072"
     CDS             complement(9300..10700)
                     /locus_tag="NOA01_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HCI0632284.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            10918..12579
                     /locus_tag="NOA01_001073"
     CDS             10918..12579
                     /locus_tag="NOA01_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HCI0632285.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            12536..13234
                     /locus_tag="NOA01_001074"
     CDS             12536..13234
                     /locus_tag="NOA01_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCI0632286.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            13250..14674
                     /gene="araA"
                     /locus_tag="NOA01_001075"
     CDS             13250..14674
                     /gene="araA"
                     /locus_tag="NOA01_001075"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCI0632287.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            14981..16140
                     /locus_tag="NOA01_001076"
                     /pseudo
     CDS             14981..16140
                     /locus_tag="NOA01_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            16258..18117
                     /locus_tag="NOA01_001077"
     CDS             16258..18117
                     /locus_tag="NOA01_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCI0632288.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            18227..18661
                     /locus_tag="NOA01_001078"
     CDS             18227..18661
                     /locus_tag="NOA01_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632289.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(18920..19081)
                     /locus_tag="NOA01_001079"
     CDS             complement(18920..19081)
                     /locus_tag="NOA01_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632290.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(19100..20122)
                     /locus_tag="NOA01_001080"
     CDS             complement(19100..20122)
                     /locus_tag="NOA01_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HCI0632291.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            20250..20603
                     /locus_tag="NOA01_001081"
     CDS             20250..20603
                     /locus_tag="NOA01_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCI0632292.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20615..21781
                     /locus_tag="NOA01_001082"
     CDS             20615..21781
                     /locus_tag="NOA01_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCI0632293.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(21873..22493)
                     /locus_tag="NOA01_001083"
     CDS             complement(21873..22493)
                     /locus_tag="NOA01_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCI0632294.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(22685..>22800)
                     /locus_tag="NOA01_001084"
     CDS             complement(22685..>22800)
                     /locus_tag="NOA01_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCI0632295.1"
                     /translation="LSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
BASE COUNT         7395 a         3918 c         4802 g         6685 t
ORIGIN      
        1 aaataagtaa gtttaacatt tatattattg acaacgcttt caacgaggtg tattattcat
       61 gtataaatta aaggaggtca gttatggaag ccacaatgtt gattagtagt gtcaagcgtg
      121 taggcaaagt tgatccgcgg ttgtacagtt catttattga acatatggga cgagcagtat
      181 atgaagggat ttatcagcca gatcatccat cagctaataa aagtggattt cgccaagacg
      241 tcattcactt ggtaaaagaa ttgaacattc ccatgattag atatcctggt ggaaattttg
      301 tgtcaggatt tcgttgggaa gacagtattg ggcccatagc aaatagacct agaaggttgg
      361 atttagcttg gcggacaatt gaaacgaatg aagttgggat tcatgaattt tataactggt
      421 gtcagcatgt gggggccgaa atcaatatgg ctgtcaatct gggtactcgt ggagtagatg
      481 cagctcgtaa tttagtcgaa tattgtaatt ttcctaaagg aacttattgg agtgatttaa
      541 ggcgttcgaa tggtcaagag agcccttttg gaattaagac atggtgttta gggaatgaaa
      601 tggacggtcc ttggcagatt ggacataaaa cagcggaaga atatggacgg ttagcagagg
      661 aaacggctaa ggcgatgaaa ctcgtagatg attcaattga ggtggtttta tgcggaagtt
      721 cgaatcggca aatgcctaca tttggtgatt gggagttaac agttcttgat caagcgtacg
      781 atcaaataga ttatttgtct ctgcatcaat attatggcaa ccaaaaaaat gatctttctc
      841 attatcttgc acgttcctta gacatggatc ggttcatttc tgaagtgaca gcgatgtgcg
      901 atacagtcaa ggcgaagaaa catagtaaga agcagatcaa cctgtccttt gatgaatgga
      961 atatttggta tcattcagcg gaacaagatg agcaagcagc gccttggcag attgctcctc
     1021 ctttactaga agataactat aattttgaag atgcattact attaggctgt ctattaatca
     1081 ctttattgaa acatgcagat agagtaaaga ttgcttgtct tgctcaattg gtaaatgtta
     1141 ttgcaccaat caggacagaa acaaacggag aagcttggag acagacgatc tattatccat
     1201 ttatgcaagc tgctgtgtat ggacaaggag aggtgttaag cccacaaatt cgttcagcaa
     1261 actatgcgac ggaagatttt gaagaagtgc cgtatttaga aagtatagcc gtaataaatg
     1321 acaaagaact tgtcatattt gcagttaacc gtggagaaga agaaatgaat ctaactgttc
     1381 acttgtctga attatcatta gagggagtga ttgatttttc tgaaatgagt ggatttgata
     1441 caaaagcaac taatcttcct aaaagtgaac aagtaaaacc aagtcattca acaaatgtaa
     1501 aagctgaaga tgggaaaatc aattgtttat tgaaaccgct atcttggaat gtggtcagat
     1561 gtcgtttatc agatgaagga ggaacaaaaa atgaaaggta aaaaatggtt aattggctct
     1621 ttgttggtag ctgcaacagg tattttagga gcttgcggtg gcggaagttc agcagaatct
     1681 gattctgata aagaagtgac tttgactttt tggaatgggt ttactgcatc tgatggtgaa
     1741 atattgcaag aaatcgttaa tgaatttaat aaaacaaatg ataaaaacat caaaattgaa
     1801 atggatatca tgacttgggc caatttgaat gaaaaacttc ctacagcgat ttcttctaaa
     1861 aaagcacctg actttattgc attaaattat ccagattttg cacagtatgt agaaaacgga
     1921 gcggtacaac cattagacga tttctgggaa tatgatggag tagataaatc tgattttaaa
     1981 gaaacggcta ttgaattagg tgaagtagaa ggtaaacagt attttgtccc catgcaggta
     2041 caaggcatgt atatgtattg gaataatgat ttgtttaaac aagctggatt agatactgaa
     2101 caaccaccta ctacttggga agaattgaca gaaatggcac caaaattaac tgatagtagt
     2161 aaaaatgtat caggttttgt cttcaataag gatggaactg caccgttata taactggatg
     2221 ttagcaaatg gcggtaaatt agtgaacgat gattatacta agtctgaatt tgcgtctcct
     2281 gagaacctta aaacgttaaa agcgatccaa aaaatgatcc atgtagacaa gactggtcca
     2341 gaatcgattt ctggcgccga aatggacaat ttaatgaatg ctggtcaact agccattgaa
     2401 atcaatgggc catggttaaa taacggattg aaagcaaacg agattgatta taatgtgact
     2461 accttaccac aagggagtga ttcagacaaa aaaacagcta ttttagatgg cgtaggatat
     2521 gccattccgg caagtacaga taataataaa aaagaagctg tctatgagtt tttgaaatat
     2581 tggaatacaa ctgaaattgg taaaaaatgg agtatcgaaa atggttttcc tgcctacttg
     2641 gattcagtag caaaagatga agaagtaaaa aatgatccta ttgtatctga attatcgaaa
     2701 caaatggact atgcagagcc atatttacca ggttttaccc aaatctctgc gatcaataac
     2761 gatatcatca atccactgat tgaaaaatta ttagcaggag aagatcctga gacactcatg
     2821 aacgatgcag atgatgccat caatcaatta ctgcaaaaat agtacagaac tttatacagg
     2881 atagactgtt ggcaagatac agatatctga atgatcgacc tagtatttgc agataggtgt
     2941 tattgcaaaa atcacagcga ttgttcggat atcttattca gattcgttta aaccaagatt
     3001 gtatatttta tcaaaatgaa agatgaggtg gactatatga acggattcac caaatattgg
     3061 aatcgtcctg atcgtgctgc gtatttgttt ttaactccat cttttttagt tttattatta
     3121 tttactgtca taccgctttt aggaacattt gctattagtt ttacagattt gaatattttc
     3181 tttacatcaa gaaattttgt aagttttgaa aatttcacac gtatatttag tgatgaacga
     3241 gcagttaatt cgttgattca ttctttatat ttcaccgtac tcgaaacgcc aacacaaatt
     3301 atcgttggtt tattagcagc agtagcatta tctaaaaata ccagattcaa tcgtttttgt
     3361 cgttcgacat tttacattcc agttatttgt tcattgacgt caattgcgat tattttttct
     3421 atgttgcttg atccgaatgt aggggcgatt ccttacttgt tttcacaagt cggcttgccg
     3481 atccctcaat tttttcgcga tccaaccctt gctatgccga cggtagccat catgactgtt
     3541 tggaagaatt ttggtgttac tatgacgatt ctcttgactg ctattcaggg aatctctccc
     3601 tctttgtatg aatctgctca aatggatggt gcaacaaatc gacagcagtt ttttaacatt
     3661 acgctacctc agatcgttcc ctccttaggt ttttgtattt taacgaatct gattggttcg
     3721 atgcaagtgt ttgaccaagt atatgttgca accgctggtg gcccacaatt taagacagaa
     3781 acagctgttc aatatattta cagtcgagga tttacagcac cttatgaatt gggctatgca
     3841 tcagcactgt cttcggtttt gtttatcatt gttgcaacta tagctatctc cactaatgtg
     3901 tacatgtcac ggaaagaaaa acaaatgaaa taggagggaa acctgtatgg caccagaaag
     3961 ctcgattaca gttactgtga atagtcgaaa agcagttagt ggtaaaaaaa tcattcgaac
     4021 gattagtatg ctagttatat tgttggttat tgcgtttcct tttttgtggc tgattatttc
     4081 ttcttttaaa cacgaaaaag acattatctc atttccgcct agaatatttg cagatagcta
     4141 taccctagat aactatatta aggtttggac aacgatccct ttattagatt acatcaaaaa
     4201 tacagtgatt tttgctggag ggacagtgat cacttctgta ttctttgatt ctcttgcggg
     4261 gtatgcattt gctcgtatgc gtttcaaagg aaaatcagtt ttgttctatt tcgttttgct
     4321 gacaatgatg atcccattcc aagtcttcat gattcctcta tttattgaag tcaatttgct
     4381 tggcatgctt gatacttatg caggtttgat cattcctcgt atgacaactg catttggtat
     4441 cttcatgatg cgttcctttt ttattacttt accagattct ttggaagagg cagcacgcat
     4501 tgatggatta agtgagttta atattttttt gaaaatcatg ttgccattga gcaaacctac
     4561 tcttttatca ttagggattt tcacgctgat gaatagttgg aatgacttgc tttatccatt
     4621 gatattgaca agtagttcaa aaatgagaac tttaccagca gggttagctt tatttacagg
     4681 gcagaacatt tctttttatg gacctgtaat ggctggaacg gttatttcaa tgctgccatt
     4741 actagtggtt tatatttttg cacagaaata ttttgttcaa ggaacagcta tgtctggaat
     4801 gaaagagtga ggaagatgaa aatagttgag actaaacttt atggacagtt gattgtggtt
     4861 tctgattaca tcttattagg tattctctgg gtaatcgtgt gtttgcctgt tattactatc
     4921 gtgcctgcta catgtgctgt tttttatgta atggcgcaat ggaaaggtga gggatcagga
     4981 aatatcattt attttttctt tcaaggaata aagcaacatt ttggtaagaa cttgttcctt
     5041 agtctgttaa cgatcattat ttattttctt gttaatcaat tattagaaga acagacaatc
     5101 gtactttcaa ttagtggata tgtagtttta cttatttatt ccatgtttct tttatcctgg
     5161 atatatgtga tttctgaaaa caaagatatc acatttttac aaatttttga acaaagtgca
     5221 tggaaagtat tttttgattt tttgagaaat attctttgtt gcgtattgtt attaggattt
     5281 gtactgctca tatttctttt ccctcctttt atttttatct ttgctggtgg cgtatggaaa
     5341 cttgtatcta ttatcttgac tataggaaat gaaaggtagg gaaagctgtt ggacgaagaa
     5401 gtgaaagtaa aaatcagaga ggattttacg gtaggagaga tcagttctcg gctgtttggc
     5461 tcatttgttg agcatatggg aagtgtcgtc tatacaggga tttatgaacc gggccatgaa
     5521 acagctgatg gtgacgggtt tagacaagat gtcctctctt atgtaaagga attagaatta
     5581 ggtgtcattc gctatcctgg aggaaacttt acatctggtt atgattgggt ggatactgta
     5641 ggtaggaaag aacaacgacc aaaaaaaatt gatttagcat ggcgaggtat agaaccaaat
     5701 tcttttggac tccacgaatt ttttaaatgg atcagtcatg tacaagcgaa accgatattc
     5761 acggtgaatt taggaacaaa aggaattgat gaagcaaaaa atttagtaga atattgtaat
     5821 tttccaaaag gaaccttctg gagtgattta agaataaaaa atgggcaaaa aaagcctttt
     5881 ggcgttaaat tatggtgttt agggaatgag ctagacggtc catggcaagt gggggctaag
     5941 agtgcttatg aatatggtag gttagcaaat gaagcctcaa aagtcatgaa gttagtggat
     6001 gattcaattg aaacaatctt agttggaagc tctacgccac gtttaccatc gtaccctgaa
     6061 tgggatcgta tcgcattaga agaagcttat gagaatgtag attatttagc tttgcataac
     6121 tatattgata agtatgatga agaagacttg actaaacctc ctaaaaatga gcgtgatgat
     6181 acagcgacat accttgcaaa agcttatcga ttcgatcgtc aaattgaaga aatcgttgct
     6241 acttgtgatt atgtaaaagg atatttgaga agtgagaaaa caatgtatct agcgtttgat
     6301 gaatggaatg tacatgccca accggaaaaa agacataggg attttgaagt cggtagtccc
     6361 atcgattggt gttactttac tatggaagat acattattat ttggttcatt agggctggct
     6421 attttacgac acgcagatcg cgtaaagatt agttgtcaat cattgttagt aaacacaata
     6481 cctttaatac tgacagatgc taaaggacaa gcatggagga atccaactta ttatgtgatg
     6541 caacacttat cgaaatatgc taaaggtcaa gtattgaaac aacaaatcat ttgccctgtt
     6601 tatgattgtg aaaagataaa aaatgttcct tatgtggaca gtgtgatcgt agataacggc
     6661 aatcagctga ctattttctt gatcaatcgc acaaatgaac aacaaaagat aacgattgaa
     6721 catgatttca atttgctaga tcaaggagag cactacacat taaccagtag cgatctgttg
     6781 gacaaaaata cacgtgaaga accagaaaaa ataaaacctg aaataaacta tttttcaata
     6841 gaaaaacatg acgaattaaa tgtagggata gaaaaatact cttggaatgt aattagaata
     6901 aagaaaatgt gaggaaattt gttatgatga agacacttta taataatcct atcgttcggc
     6961 aacgggcaga tccttgggta tataaacata cagatggtta ttattatttt acaggatcgg
     7021 ttccaggcta ccaagtgatt gaattaagaa gggcaaagtc actgaatgaa ttagaacatg
     7081 ctgcaagcct gattgtatgg caggctcatg aagaaggacc gatgagtgaa ttgatctggg
     7141 cacctgaagt ccattatatc aatggaaaat ggtatattta ctttgctgca agcaatcata
     7201 aaactatacg tgatgaatct catcatcacc gcatgttcgt acttgaaaat cagcatatag
     7261 atccaatgaa tacagattgg atagaaaagg gacaaataaa aacgaaaagg gaaactttca
     7321 gtttagacgg aacaactttc tcttctcaag gaaagctata ttatgtgtgg gcccaacaag
     7381 atcctgaaat tactgggaat tctaatcttt atctatccga aatggataat ccttggacgt
     7441 taaaggggaa gcagatcctc ctttctatac ctgagtatga atgggaaaaa cggggttttt
     7501 cagtaaatga aggtccagca gtccttactc gcaatgggaa aatattcatt acgtattctg
     7561 gtagtgcaac agatgaaaac tatgccatgg gcctgctgtg ggctgatgag cacaagaatc
     7621 ttttattaaa agaatcatgg cataaattat cggagccagt attcgtttct tctgaaaaaa
     7681 ataagcaatt tggtcctggt cacaactcgt ttacggtttc agaagacggg tgctttgata
     7741 ttttgatcta tcatgctcga ccagagaaga accaaaaagg ggatccgtta gacaatccta
     7801 atcggcacgc gaacgcgcaa tgctttacgt ggaatgagca aggatttcca gaatttggtg
     7861 aacctgttcc gtatgcctta tgagcacaag aaaccttgat ttttgcttga ataaaccttg
     7921 cagactcgtt atgatatatg tacgtacaaa taaaaagggg caaggcaaat ggcaaaatat
     7981 caagatattg cagaagagat aaaacaaaag atccttcgtg aggaatacga agtaaataca
     8041 accattccct cagaattcaa gctgcaagaa atatatgacg tcagcagaca tacgattcgg
     8101 caagcaatcg ctgtgttggt aaatgagggc tatctgcgta aagaaaaagg gtcgggcaca
     8161 tatgtagatg acagctataa gaaagaaaaa acaccattga acagaacaaa aacgatcggt
     8221 gtcatcacca catatctctc tgattatatt tttccgtcga ttatccgagg aatcgaaaat
     8281 gaattacgag aatctgggta ttcattgctt ttagccagta cgaataacga tcacgaattg
     8341 gagaaagagt gtttgaagaa aatgattgct tatggggtag atggtttgat cgttgagcct
     8401 acgaaaagta atcagtataa cccaaactta gcactttatg ttgctttgag agaacaaggc
     8461 attccgatgg tgatgatcaa tgctgcttat gaagagttaa atgcctctta tatttgttta
     8521 gatgatgtga aagtcggttt tatggcagca gaatatttga taaaaaaagg acatcagcgt
     8581 ctgttgttca tcacaaaaat cgacgatctt caaggaaaat acaggatgaa aggttttatc
     8641 agcgcgtgtg aacagtataa tgtgcgtttt ttgacggaag atatcattac tttcaccacg
     8701 gaaacacgag aaacaatcgc tgaaaaaaca gtacaacggt tgaaagaaga acaagcttta
     8761 gatggcatca tttgctataa cgatcaaata gctagtttaa tgctagataa gttgacagaa
     8821 aatggctatc atattccaga tgacctatcg atcgtaggaa cagacgattc ttctttaagc
     8881 catttaggga ataaaaaatt gacaacattg acgcatccaa aagaaaagtt gggacaagat
     8941 gccgcacggt ggatcgtcac aacgattgaa aaaggacagg cacagccaaa tattctctat
     9001 gaacctgtac tagttgaacg ggaaacagtg aaagaaaacg aaaaataaga gacgggtttt
     9061 taccgcctct tattgctcct acggttactc gtcgcaatgt ttgtgagagc ttttgtcatc
     9121 gctcaaggct gaacgtttct ttcaccacct ctccactaaa cggtgttctc ctaccaactg
     9181 gtcacaagtc acaatattca tcaaacatga gaagctgttt tttgaaattg ttccttgtga
     9241 ctggaaacta agcggttcct tcttatttct tgaagctgac cacttctgtc acaacctctt
     9301 taagaatgat ggatagaaat acgtttagga taagtttttt tagatgcact ttgttcgatt
     9361 tcctctaaag atttcccacg agtttctggt acgatctctt gaataaataa aacccctaaa
     9421 aagcagatga tcccaaaaat gccgaaaacg atttcttgag acatagaagc agtcatcaca
     9481 ggaaaaagca agccaactag gaaagaacca atccaattaa aagaagaggc taggccagat
     9541 gctcgcccgc ggacagctag tgggaatatt tcgcctacga ttacccatgt cagtggagcc
     9601 catgtgcaag aataaaatgc gacataaata cataaaaaca agagaatcaa caacgaattt
     9661 gtatgagcat ccagtacagt tcctaaaaca gctggtagga tgaaagaaag tcccatcacg
     9721 ctaccgccta ttttcaataa agttcgccga ttgaacttgt ctgcaataac caaaaagagc
     9781 aaagaaccag caactaaaat gattccttgg atgatcggcc acatcaatgc atcgctcgct
     9841 gcatgcccag tcgctttttc tacgatcagc ggaatatagt aaaaaatagc attagctcct
     9901 tgaaattgct ggaatactgc tacaccaata ccggcaatga ctaaggaacg atatttttct
     9961 aaaaacaaag tatgccaaga agttccggtt gtcttttctt gtttgacatt cagctggatt
    10021 tgttcgaatt cctgttcagc ttcttttggt gtccgaatat agttcaatac tctttttgcc
    10081 tcttccaagc ggccagattg cataagaaaa cgcggtgact ctggtagagc taaaacaccg
    10141 aaaaataaaa tcagcgcggg gacagcagcg agccctaaca tcacgcgcca agccatcgtt
    10201 tcaggcaacc cttttagcaa ataatctgca acataggaaa ggagcatgcc agaagcaatc
    10261 atcacttgat tgatccctga caatcttccc cttagtctag caggagccat ttcagacata
    10321 taagcaggaa ctagggcaga tgcagcgcca acagctaaac ctagaagaat ccttgctgca
    10381 atcagaaaat aagaaccatt gtgaggggca gatgcagaaa ggacagaacc agccataaaa
    10441 ataagtgcag aaagaagaat catttttcgt cgtccaagct tatcagaaag aagaccagat
    10501 aaagagccgc caaggatcgc acctaacatg accgaagacg tgatccatcc tatcaaagaa
    10561 gctttacctg ccaaacccca atcatgctgt aaaaaaggta aggcgccggt cataacacca
    10621 atatcatatc caaatagaat gccgccaaat gcgccaaaaa aataaataaa cgatgaggaa
    10681 actttttttt cttttttcat aagtattctc tccttctaga atttcgagaa ggcggttgat
    10741 cagattttgt aagcaatttc attttataac ttatgtacgt acaaaacaag tgttttttat
    10801 tgtattcttg taaataactg cttgacatgt acgtacaaaa gtatacaata atcattgtaa
    10861 gcgctactaa aaagacggtt tgatcaggtt tgacgagaag gagggagtta aatcattatg
    10921 aaggaagtaa atgcgcaaag tatgttagat gtcatagatg gtcgtacatc tttagggatt
    10981 gagtttggat caactaggat caaagctgta ttgattgatt catcttttac tcctattgcc
    11041 gctggtagtt atgagtggga aaacaaacta gaaaatggta tctggaccta ttcattagaa
    11101 gatatttgga aaggactgcg cgtcagttac cgaaaaatgg cagcagaagt attcgaaatc
    11161 tatggcgaga atcttacgac aatcgggtcg attgggttca gtgcaatgat gcacggttat
    11221 ctagcatttg atagcaagga acagttacta gtaccatttc gtacttggcg aaatacaatc
    11281 actgaagaag cagaacgaat attaacggaa aaatttcaat acaatattcc gcagagatgg
    11341 agtatcgctc atttgtatca atctattttg aataaagaag aacatgtgaa agaaattaat
    11401 tttttgacaa cgcttgcagg ttatgtccat tggcagctta ctggtgagaa agtactaggc
    11461 gtaggagatg catcaggcat gtttcctatc gatccttcaa cgaaaaccta ccatcaagaa
    11521 atgaaaaata tttttggtca gttaatcgaa aaagcgcacg tagcgattga cttggatcac
    11581 attttgccta aagttctagt agcaggagaa gaagctggca agttaagcga atcaggggct
    11641 tatctgatcg accctacggg aaatttgaga ccaggaatcc cggtttgtcc gccagaaggg
    11701 gatgcaggaa ccggtatggt cgctacgaac agtgtagcca aagggacagg gaacatttcg
    11761 gctggtactt ccgccttcgc tatgatcgta ttagaaaaag aactagaaaa tgtccatccg
    11821 gaaatcgaca tggtcactac accttcagga gaactagtag gcatggttca tacgaataac
    11881 tgctcttcgg atatcaatgc ttgggtcagt atttttgaag aatttaccaa ttctttgggt
    11941 atggagataa gcagagataa attattcacg gtattattca acaaagcgct agaaggcgat
    12001 acggattgcg ggggattact ttcttatggt tatttttccg gtgaaaacat tacaggagta
    12061 aatgaaggaa ggccattatt tgtacgtaca ccaggtagcc gttttgattt agcgaatttc
    12121 atgcggatcc atttagccag tgcttttggt gcaatgagaa tcggaatgga tatattgaaa
    12181 tctgagaatg tccaaattga tcgtttagtg gggcatgggg ggatttttaa aacaccagaa
    12241 gttggacagc ggattctagc atctgctatg gaggcaccag ttacagtgat ggacacagcc
    12301 ggagaaggtg gagcgtgggg aatcgcgtta ttagcagcct atatgatgga tcgacatggg
    12361 aagtctttag aaaacttctt ggcagaagac gtgtttggac aagaccaagg aatcaccatc
    12421 acaccttcag cagaagaaat agcaggctat cagatattta tgagacgtta ccgtgagggg
    12481 atcgacattg aaaaaacagc gattcatcaa ttgcaagaaa aaggagtgat agaaaatgct
    12541 ggaacaactg aaagaagaag tataccaagc aaatcttgac ttgcctaaac acggactcgt
    12601 caaatataca tggggaaatg tgagcgcttt tgatcctgaa acacgatatt tcgttatcaa
    12661 acctagcgga gtcagttatg aagagatgac agcagacgac atggtcgttg tggacttgga
    12721 caatcatatc attgaaggta aactcaatcc ctcttcggat acgcctactc atgctgtttt
    12781 atatcgttca tttcctcaaa tcggaggaat cgtccatact cactcgacat gggcgacgat
    12841 ctgggcacaa gcaggattgg atgttcctgc gatggggacg acgcatgcag atacttttta
    12901 tggatcggta ccttgtgctc gatttttgac acaacaggaa atcgacagcg ggtacgaata
    12961 tgaaacagga aaagttatca tagaaacatt caaagaacgc aaaattgatc ccttagcaat
    13021 tccaggggta cttttacacg gacacggtcc atttacttgg gggaaagatg cgcaaagtgc
    13081 ggtaatgaac gcagtcgtac tagatgaagt atgcaagatg aatttattta ccagacagct
    13141 caattcattt tcagaagagc tgcctcaacg gattttagat aaacattatt tacgaaaaca
    13201 cggaaaaaat gcgtattacg gacaaaacaa ataaaaggag tggctaatta tgttgaatat
    13261 tggagaaaaa gaattttggt ttgttgtagg atcacagcat ttatatgggg aagaagcact
    13321 gcgagaagta aagaaacatg cacaagaaat ggtcgatgaa ctgaatgaaa atggccaatt
    13381 gccttatcca atccgccttc aagaattagc agttactgct gatacgatca caaaaatcat
    13441 gaaagaggtc aattatcgag aagaagtagc aggtgtgatc acttggatgc atactttctc
    13501 accagcaaaa atgtggatcc gcgggacaaa gcttctacaa aaaccattgc ttcatttagc
    13561 tacgcaatat aacgaaagca ttccttggaa aacgatcgat atggatttca tgaatttaaa
    13621 tcaatccgct catggtgacc gcgaatatgg gttcatcaat gctcgattaa ataaacaaaa
    13681 taaaatcgtc gttggctact ggaaacgacc tgagatccaa aaagagattg ctgattggat
    13741 ggatgttgca gtagcctata atgaaagttt tggaataaaa gtggctcgtt ttggtgacaa
    13801 catgagaaac gtgggtgtca cagaaggaga caaagtggaa gcacagattc aatttggttg
    13861 gactgttgat tattttggta tcggagatct tgtacaagta atcgatcgcg tatcagacga
    13921 agaagtcgat caattatttg aagagtacaa ggaattgtat accttcgact atggtgatta
    13981 tgaagaaaag acatgggagg aacatgtaaa agtacaagct cagcaggaaa tcggtattcg
    14041 ccgtttctta gaagaaggag gctataacgc ttttacgact aattttgaag acctgtatgg
    14101 aatgaaacaa ctacctggac tagctgtaca acgtttgatg gcagaaggat acggttttgc
    14161 tggggaaggc gactggaaaa ctgcagcgat tgatcggttg ctgaaaatca tggctcgagg
    14221 gaaagataca ggattcatgg aagattatac ttacgagcta gctagtggcc aagaagcaat
    14281 cttggaatcc catatgatgg aagtagatcc gacgcttgct gcgacgaaac caagaatcgt
    14341 tgtttctcct ttatcaatgg gagatagaga agatccggcg cgtcttgtat ttgatgggaa
    14401 agcaggagaa ggggtagtcg tatcgatggc agatttcggt acgcattata aactgctgat
    14461 caatgaagta gaagcctttg aaccaacgac tgaagcacct aatttacctg ttgcacgtgt
    14521 gttatggaaa actaaaccga atttccatga aggtgtccat tcatggattc aggcaggtgg
    14581 cggtcatcat accgttgtct cgctaaatct aacaacagat caaattgaaa cgtgggcaaa
    14641 attagttgag ttggaaacag tggttattag ataaaaagag gttgtgaaaa agccacttag
    14701 ttttaagaaa taagaagaga ttgacgatat ttgcttttca acttttttga aatttagtct
    14761 tattatcaat gtcagctaac ggaacaccat ttatcaagat atctcaccct caatattttg
    14821 ctctcgaaaa taaggcttat ggcagtaact tgtcatagcc ttatttttat cactcagcct
    14881 actctgctct aactgctctt tttgcttcta ggaagtcaaa tggtaaacag ttgtctaatt
    14941 gcagaaattt cgagatactt gttagtaagg agggggtttt atgtatcacg ttcatttgat
    15001 tgatgctatt ccagaacaag agatagaaaa gttacaaggg gaatatttca aaataataga
    15061 tgatttgcca gccgaaggaa tagttattcg tagtacgaaa gtgaaagatg aatggcttac
    15121 accagatttg ctagcaattt caagagctgg tgttggggtg aacacgatca acgttgaaaa
    15181 agcttctgaa aacgggacga tcgtcatgaa tacaccaggt gtaaatgcta atgcagtaaa
    15241 agagcttgtt ttgtgctgtt tgctcttatc ttcccgtccg ataatcgaag caagtcgtat
    15301 ggtacaaaca ctaacaggtc ctaatatttt agaacaggca gaaaacaaaa gaagtgctta
    15361 tgtgggacga gaattgcaag gcaaaaccat cggtcttctt ggtcttggag cgataggcac
    15421 aaaagtagcg ctaagttgct atagcttagg gatggatgtc ttgggttatt ctatccgcga
    15481 tgcccagttg gattatgttc ggcaagcaga tttagaaaca gttctttcta cctcggatta
    15541 catcgtagtc atgctgccgt tgacagaaga tacaaaagga ttgatcgatc aggcgaatat
    15601 agaaaaaatg aaaaaagatg ctgtacttat caatgtagga agaagtgaaa ttgttgataa
    15661 atatgctgtt atgcaagcat tggaaaaaca acatctggct aaatacttga ctgattttcc
    15721 agaagaagag ttcttagaaa atgaccgtat acttatgttg cctcatctag gggatcgact
    15781 caggaagctt tggcagacag cggacgtttg gcagttgaag ccttaaaaga ttatcttttg
    15841 ttcggtacgg ttagagaagc ggtcaattat ccatccgcac gaatgttttt ccaagcgccg
    15901 tttcgattca ctatatttta tcaaaagaaa accaatatat tagcagagat attttctctg
    15961 ctgaatgaga acgagctagc gatcgcagat atatcgcgta accataagaa tgagcatgtc
    16021 tacatattga ttgacataga cagtactgat tttgaacagc tcaatcaagt gaaacaacag
    16081 ctagaaaaaa tttctggagt acgaaaggtc cgcttattaa agaaaccaga aaggccataa
    16141 agaaagagaa tatttggatt tcattaattc ttctttccgt tctttacttt cgaatttcgt
    16201 acaactaaaa taagagaagc aacattcttt tattgataga aatgaaggag caagcatttg
    16261 aaaagatata taagcagact ttcgaccaaa cagaaaaaga ttttaaaagg aacagcagca
    16321 atcatcagta tcttttttct tgttgttttt agtaatttat ttttgcagtg gtgtcaaaat
    16381 gatctatccg tagaccttgc gttgaaattt gctttttctt ggcatacgga aaaattcttc
    16441 ttagcctgtc tcgtcttgct gattattctt atttttctga ttgcactagc aggatctgtc
    16501 cctttaggtt cccttacgta tgtcgtagcc atcggtgtat taggttttgc aaattatatg
    16561 aaaatgagtt atcggcaaga accgatttat ccagacgatt tgaagatgat cactgaaatc
    16621 ggattgctga aagatatgac cggcactatg ctgtttacag tgatcttagc tgccgcagga
    16681 accgtgatag gattgttttg ctggtacatg tttcgaagcc tgaaaaaagg aaggcgattc
    16741 caattgatcc gattaacgac actgcttgtg gcaattggac tgctagggta tatcagtaat
    16801 ttcaacaatc ccgataattt gttgcgaaaa gcatataata aaacggcact atggatacct
    16861 tacagccaga aaatgaacta ctataacact ggttttatcg gtgggttttt gtacaatctg
    16921 aaggtagaac caatggatga acctgaagga tattcgaaag aaaagatcaa agaaatcact
    16981 gaaaaatacc agaagctagc cgatgaaaaa aataaaactg tagaggaaga gtcgccgaac
    17041 atcgtgttcg tgatgagtga aagtttctca gatcctagtc gattaaacgg cgtagaagta
    17101 tctggtgagc cattggcaga ttattatgaa gtagctgatc aaacatatag tggcaatatg
    17161 ctttctcaaa actatggcgg tggaacagca aatatcgaat ttgaagcatt gactggtttt
    17221 tctatggcgt tatttaatgc gcaactcacc accccttaca cgatgcttgt accaaaaatg
    17281 gatcagcttc cttctatcgt ttcagcgtta aacgcacaat cataccagac gacagcgatc
    17341 catccataca atacttcgat gtataaaaga gaggatgttt atcagacatt aggatttgac
    17401 cagttcatca gcgagcgtac gatgacctat acggatacga tagagaataa tccatatatt
    17461 tctgatgagt cagcttacaa agaagtaatg acacttctca aggaagaaaa aacacctcag
    17521 ttcattcatt tagtcacgat gcagacacat atgccctacg atggaaagta tgataatcta
    17581 gactatcctg caaaaacttc agatggaagt gggacacttg acttagagaa ttatctgcaa
    17641 gatatttcct atagcagcac ggcactgaag cagttcacgg aagaattgaa gaatctttct
    17701 agaaggactt tagttgtttt ttggggggat catcttccag ggatctattc agatacgatc
    17761 caagcaaaaa atgacaaaca aacattgcat gaaacacaat ttcttatgtt cgacagtaaa
    17821 ggagaattag aaaagcaaac gactcacgat gcaatcacca gtccgtttta ttttgcagcg
    17881 aatctaatgg aacagacgaa tcagacaaca aatggctttt atcagctgct tttatcatta
    17941 gaacaggaac ttccggcgtt tgaacgagaa ctgtactatc aaaacggaca gtggtataaa
    18001 gaagcgcaat tcaatcgctc gcaacaagag atttacgacg aatatcaatt gatccaatat
    18061 gacattgtcg cagggaaaca atatagttta gcagatggtt tctttgagca cgaataagaa
    18121 tggcgagctg tatagaaacc attaaatttt ttcaagtact tgtcatttga ggataaaaac
    18181 aagtagaata aataataaca acaatggaga aagtaggaaa acgaacatgt taccgaatat
    18241 tggctattat gtgcagtcca tcgatcaaat cgtcaaggaa acagaagcaa tcggagaagg
    18301 aatgcatccg aaatatgaag tgatcagaaa cgcaattgat gaaggaaaaa caagtgaact
    18361 ggacaaatcg actcttgaag agatcatcct gttatttgaa gaaggaacaa aaaaatatcg
    18421 ggcaatgctt atgaaagtca gtactttgcg tccaccagca aaagcaatgg gcattcataa
    18481 aaattttgaa aaagcttatc tttcatatgt agcaggctgt gaagaaatga tccaaagtat
    18541 cgatcttgaa tcaggggtag atgaagatct attcaataca tctgaagcaa aacaagacca
    18601 agcgacagac caattgacag ctgccatcca aaaaatgact aatttgttgt tgaaaaaata
    18661 aaagaaatct ttgataacaa taaaaactag agttatctaa tataaatgta atattacctt
    18721 tatgatagag tgatcaaaat cctttatgat aatgacatac ctataaccaa caacaacctt
    18781 gtacttactc aaaatcgagt aaatactcct tttccgtcga gtctaaccat ctcggcggtt
    18841 ttttatttgt agaaaagaac atcaggatag acggaaaagt cttgtcctga tgttcttttc
    18901 agatagtatg gaaagctgat tagttttcta cattctgtac atattgttca aagtagttct
    18961 gtaaatcggc tttgagtttc tgatattcct cttcgctata ctctttatct tcgattttcc
    19021 catcaaagaa aggaatattt agttcttccc gtagtctgtc taatacgtct tgctgattca
    19081 tttcattctc cttttcttac taacgatcaa aataaacaca gacacattct ggtacataga
    19141 catctttttt cagtaattcc agtaatcctg tttcaggatg acggcgatat aatgtcagat
    19201 tatcggtgtt ctgattggca cacacgacaa aattattgct tggatctagt gcgaaatcgc
    19261 ggggaaaatc cccttctgtt gagatgattt gcttgttttg aatgtgggaa ccatcttcaa
    19321 aaactgcgaa tacagcaatc gagttatgtc ctcggttaga agcataaagg aacttgccat
    19381 catttgaaat gcggatagct gcccctccat tttcaccgtc gaattcttct ggcaaggtcg
    19441 atattttctg taattcttca aaagcacctg tttctttatt ataagaaagc acgcggactg
    19501 tgctgtctaa ttcaccaaat aaataagcga attgtccatt tggatgaaag acaagatggc
    19561 gagggcctgt tcctggctct gcagtgaagg tagttacaag attcaatttg ccgttttcgg
    19621 acacatcata agtatacaca cgatctgtac caagatcaca tacgactaat cgttggtcag
    19681 gagtcagatc cgtatagtgg acatgcgcat gatcttggtt tttatgagga cctgtcgctt
    19741 cttgatggaa tattgaatct gctgctttca gttcgccatc tcctaatata tggtagacat
    19801 ttacttcacc tttatgatag ttcgccccgt atagtaattg acgtgcttca tcaaccgcta
    19861 cataacacaa aggagcgcca gactctgtta ccgcattcaa aaaatgaaaa tccttatcgt
    19921 acgcaccggc accacctaat ccatccacac tagtaactgt gtaaagattg ccagctttac
    19981 tttttgctac ataggtaggg ctattttctt caacagctaa tgaaaggttg gctaattctt
    20041 ctttttctgt atcaaggtcg atcgtataga ttcctttact ttcgcgacgc gtataggttc
    20101 ctaaaattat cttttcgatc atatgagctg attcctccta attgtaatga aatagttttc
    20161 gtttttattc tatcacataa ttattccaca cggctaaaga atgagaatgt gttatactgt
    20221 ttttgaaagc aattaaagga gagagtgcaa tgattcaggc aaagattaca gaaattggga
    20281 cagaagcaat aaacaaggaa gaacccatgc tgattttgtt cgataaaaca gcaacagctg
    20341 ccttacgaaa atattcggtg attcaagaag tatcaaaaga cgaacctttt tctttgaaag
    20401 caggcggaac aattgctttt gatgatcagg tatatacgat cgattatgtc ggtccgatgg
    20461 ccaatgggaa tttaaagaca gtagctcacg tatcattgat ttttgatgct tgtccagaag
    20521 aaggacaaat tgcaaatgga atctatcttc atccgcatag actgcctgct attggtttgg
    20581 gaagccaaat tttttataac taacaggagg aattatggaa ggaaaaaata agaagaacag
    20641 accggtatcg tggttttggc gctggttttt aaataatcaa gtagtcacag ccttacttgt
    20701 tgtattgtta atattgctga tcgtcttttt atttacaaaa gtttcatatt tgtttgcacc
    20761 aatctggcaa tttttagcaa tcgtaggtct gcctatcatt ttagcaggaa tcttgtatta
    20821 cttgatgaat ccggtagttg attactttga aaagcgaaaa gtaccaaggc tttacagtat
    20881 cattgggctg tttgttttgg ttgttgcact aatcgcatgg gggagcgtag tgatcatccc
    20941 taagattcag gaacaaacag tgagcttcat cagtaacttc cctcaatatg tagatacgat
    21001 tgataataaa ttaaccgaga ttttgcggga cccgctgttc aaccaatttc gcgaacagtt
    21061 ggaaacagca ggtgataagt tcatgaattc ggcaggcgat atgatccaag atatatctaa
    21121 gagtacagtt cagagtcttg gaagttttgt cggcgcggta gcgactattt tagtggctct
    21181 gctgacgatg ccgtttatct tattttatct attgaaagac ggtaaacaat tagcacctta
    21241 ttttgtcaaa tttttaccga cacgcatgca aaaaccgacg ttgaatgtgc tgagtgaaat
    21301 gaatagtcaa gtttcttctt atataagagg acaactaact gtggcttttg ctgtggcaat
    21361 catgtttatg attggttttg cggtcattgg tttagagtat gctgtcacgt tggggattat
    21421 tgcgggtttt ttaaacctga tcccttactt aggatcattt ttagcgatga ttccggctat
    21481 ctttttagcc attgtagcag gtccattcat gatcatcaaa gtactgatcg tttttgcagt
    21541 agagcagaca atagaaggac gcttgatctc tccattagta ctagggaatc agttagcaat
    21601 ccatccagta acgattttat tagttctgct gacttctggg aaattgttcg gaatcatcgg
    21661 tgtgatcttg gggattcctg tttatgcagc agctaaagta gtgattactc atgtttttga
    21721 atggtacaca acaatctctt ctctttatga agaaaatccg ttgaaaaagc agaaagaata
    21781 acaaaaagag acagatggga aaatatccaa ggcacaaatg ctgctttgaa gattttttcc
    21841 tctgtctctt ttgtatgcat ttatctcaat ttttaaaatg atttacctaa agcagcagct
    21901 ttatctaatg cattttgcat caactcatct gcacggtcag ggtcataatc gattccttcg
    21961 atgaatagct gatctacttg atcgatgcct acaaagttta aaataccttt tacatattga
    22021 gaggcaaagt cttgtccgtt gtatacaccg ccattagatt ggatgtgcag tgctttttta
    22081 cctgtcacta atccttctgg cccgttttct gtatagcgga acgtttttcc agctacattg
    22141 acggtatcga accaagcttt taattttgtt gggatattta gattccataa ggcgtttgcg
    22201 ataacgattt tatcagcagc aaggaactga tcagtcaatt cattgaaacg agcaattttt
    22261 tgttgttgac tttctgataa ggaagtgaat tctgcaccag cacgtaatgc ttcccaacct
    22321 gataataatt cttcatcgat ttccggaata tcatcgcgat aaagttccaa aacagtcact
    22381 tcatcatctg gatgattttc tttataactt gttaaaaaag acgtcaatgc cttaactgtg
    22441 cgcgattctt ctgcagttag gggatgacct ttgactacta aaacagatgc catatgctta
    22501 ccttctcctt ttattgctat gattgttata actatgacta acaaaatcag tttactcgaa
    22561 aaagaaaagg ctatcaagtc atattgataa ggttgaagaa taaataacta aaaaaagaca
    22621 gggtataaaa gaggctgtga cacgattttt tgtcacagcc tcttttgaat ctaacaagca
    22681 atattcaagc aacactcgac tgaagagctt ccatcccacc aatgatataa acagcacgaa
    22741 aaccattagc agtaagttga tcggctaatg cttctgatcg cctgcctgta tagcttagaa
//
LOCUS       DAIGOK010000035        22744 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.035, whole genome shotgun sequence.
ACCESSION   DAIGOK010000035 DAIGOK010000000
VERSION     DAIGOK010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.035"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            476..1339
                     /locus_tag="NOA01_001085"
     CDS             476..1339
                     /locus_tag="NOA01_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCI0632296.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="NOA01_001086"
     CDS             complement(1468..3090)
                     /locus_tag="NOA01_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCI0632297.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="NOA01_001087"
     CDS             3339..3860
                     /locus_tag="NOA01_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HCI0632298.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5775)
                     /gene="spxB"
                     /locus_tag="NOA01_001088"
                     /pseudo
     CDS             complement(4046..5775)
                     /gene="spxB"
                     /locus_tag="NOA01_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
     gene            5940..6758
                     /locus_tag="NOA01_001089"
     CDS             5940..6758
                     /locus_tag="NOA01_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HCI0632299.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6861..7058)
                     /locus_tag="NOA01_001090"
     CDS             complement(6861..7058)
                     /locus_tag="NOA01_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632300.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7227..7805
                     /locus_tag="NOA01_001091"
     CDS             7227..7805
                     /locus_tag="NOA01_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HCI0632301.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7900..8037
                     /locus_tag="NOA01_001092"
     CDS             7900..8037
                     /locus_tag="NOA01_001092"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632302.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8850..9353)
                     /locus_tag="NOA01_001093"
     CDS             complement(8850..9353)
                     /locus_tag="NOA01_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCI0632303.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9712..10737
                     /locus_tag="NOA01_001094"
     CDS             9712..10737
                     /locus_tag="NOA01_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCI0632304.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            11084..11962
                     /locus_tag="NOA01_001095"
     CDS             11084..11962
                     /locus_tag="NOA01_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HCI0632305.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12438..12554
                     /locus_tag="NOA01_001096"
     CDS             12438..12554
                     /locus_tag="NOA01_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCI0632306.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13482..13820
                     /locus_tag="NOA01_001097"
     CDS             13482..13820
                     /locus_tag="NOA01_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632307.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13914..14144
                     /locus_tag="NOA01_001098"
     CDS             13914..14144
                     /locus_tag="NOA01_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632308.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14544..14915
                     /locus_tag="NOA01_001099"
     CDS             14544..14915
                     /locus_tag="NOA01_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HCI0632309.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(15189..15881)
                     /locus_tag="NOA01_001100"
     CDS             complement(15189..15881)
                     /locus_tag="NOA01_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCI0632310.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15884..16669)
                     /locus_tag="NOA01_001101"
     CDS             complement(15884..16669)
                     /locus_tag="NOA01_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCI0632311.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16906..18306
                     /gene="lacG"
                     /locus_tag="NOA01_001102"
     CDS             16906..18306
                     /gene="lacG"
                     /locus_tag="NOA01_001102"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HCI0632312.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            18331..18648
                     /locus_tag="NOA01_001103"
     CDS             18331..18648
                     /locus_tag="NOA01_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCI0632313.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18661..20334
                     /locus_tag="NOA01_001104"
     CDS             18661..20334
                     /locus_tag="NOA01_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HCI0632314.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20348..21979
                     /locus_tag="NOA01_001105"
     CDS             20348..21979
                     /locus_tag="NOA01_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HCI0632315.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
BASE COUNT         7102 a         3824 c         4565 g         7253 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctattat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggtgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcattagttc ctggaaagag ttatagttca tggatgtaga
     5461 agcaacttgt cctaagaacg ctactagagg cacatggtcc atctgtgcat catacaaacc
     5521 attgatcaaa tgtgtcgcac caggtcctgc agagccgaag acagcgccga ctttccccgt
     5581 taattttgca tctgcagcgg ctgcgagtgc gccgacttct tcatgacgaa cttgaatgta
     5641 gttgatttcg gttttttctt gatatagcgc atccataatt gaattgaaag atcctccggg
     5701 aatgccgtaa atatggtcga taccccagct ttcaagaact tttaccattg cgattcctgc
     5761 attgattttt gacatggtgt ttcactcctt tagcaatgat gatacataaa ttttttgtat
     5821 ctagagtata gaacttttta tcgaaaatcc caactaattc gtcttttcac aaaaatgtag
     5881 tcatgttttt gggaatatgc tagagtagta catagataga ttcaagagag gggaatggca
     5941 tggttgaatg gctattgaga cttgaacctt ggcagcaagc acttgctggg acagctttta
     6001 cttattttat gacagctctt ggtgccgggt tggttttttt ctttaaagaa atcaaaaaag
     6061 aagtgctaaa cttgatgtta ggatttgctt ctggtgtcat gattgctgca agtttttggt
     6121 ctttgttaga tccggctatt acaaaagcag aagaaaatgg agatattgct tggctagttg
     6181 taagtattgg gtttggttta ggcggtgtgt ttttatatat ggcagataaa acactgcccc
     6241 atatgcattt tggaccacaa catgaagcag aaggattgcc tacccattta aaacggacga
     6301 tcttgcttgt tttttctatt acattgcata acattcctga aggtttagcc gtgggggtag
     6361 cattcggtgc agcagcaact gcggataatc cgacagccgc tatattagcc gctgtttcgg
     6421 tggcgctagg tattggtatc caaaacttcc cagaaggagc agctgtatca atcccgttgc
     6481 gacaagaagg tctgagtcgt aaaaaggcct ttgtttacgg acaagcttct ggaatcgttg
     6541 aaccaattgc tggagtgatt ggagcgctac tagtaacgaa agtggagctg ctgcttcctt
     6601 atgcacttgc ttttgccgca ggagcaatga tttatgtcgt ggtggaagaa ttgatccctg
     6661 aagcacagca gacattgtcc agcaaacgtc attttgcagt ttttggcgtc atgagtggat
     6721 ttatcattat gatgattttg gatgtggcat taggataaat aaaaaaaata tatatgatat
     6781 atcaaatagg gccgcgaact tatatcgcga ccctattttt ttagtaagtt atatcataat
     6841 gaagtgaatg gtatcataca ttaataatag ttttttctat gtgtatttcc tttgatccag
     6901 cgaacaagcc agatgataag tgcgattggg attagaactt tgaccgcaaa ccataaaagc
     6961 ccgcttagga cactgacaaa aagactgatg acgaagctag ctgcaattag cactaaaaga
     7021 accatgaaga tatttaagaa catatttttt tgacgcatac aaaaccctcc tcttgttcat
     7081 tctttactat agaaaaagta taacagggat tgtttaaaaa cacctccaac tgaagactga
     7141 tttttacttc atctacttaa atgatcaaac aattgctttt tctctggttt atcatctaaa
     7201 atagagcgga aaaggaggag aatatcatgc ctaaaaatcg tcttgaagca tttacagatg
     7261 ctgtaatcgc gattatcatg actattttag ttttagaact tcatgaacct aacggagaca
     7321 cgtgggaggc gctcgctgca ttaggttacc gattttttat ctatattgtc agttttatca
     7381 ttttagcgat ttattggaac aatcatcatc atttatttca agttgtacat aagataaatg
     7441 gacgggtctt atgggcgaac agtttctttg tttttgcgct ttcgcttttt ccgtttgcaa
     7501 cttcatggat cagtgagtac atcaatagcc tggctccaga aatgacttat ggttttgtta
     7561 ccttgaccgc taatattgcg tattatatct tggcaaaaga acttgtcaaa gcaaataaag
     7621 gccacgagaa agcattgaag gcgatgttga ataattacaa gaaatcgtat ctatcgattg
     7681 gtttgaatgt gttaggaatt gtcttagggt atttagtagc gccagtagcc gtgctgatcg
     7741 caaacatcgt gattctagct gcttggatca tcccaagtcg taccatcgaa tcacaatatc
     7801 ggtaaaatga tccgtgttta aaatttaaga gaaaaatata gaaaaaataa ataaacgatc
     7861 aaatatccga gtgatactag tattcaaaga gggatgcaga tggatcaaaa aagaattcat
     7921 gtaggactga ttactagttt tgtcacgatg cttatttatt tggatcaagg tcatttgact
     7981 tggacagtag ttggtgtcgt aggatttttg acattattca cagcaataaa aaaataaaca
     8041 tctttatgta atctgatgtt ctatctattt aagaaaagca tgtttacagc agtatgattc
     8101 tgttgtaaac gtgtttttat ttgtggaaaa tttaattttt agaaaagtgt atcgaaaaat
     8161 agatagttag tttttccaca gtggataact tcgtaagaga taaaaataaa aaattagtca
     8221 aagaagggcg tatagatttc ttttgatagg agatatacaa cagaaatgaa taaatattaa
     8281 cgtgttttca aacagtataa aacggtgtgg tttttggaat aaaaaaataa catatctcat
     8341 tttacgcttt tgttatttta tacttaaaaa ataacgacac agaaaatacc gtgtctcaga
     8401 tttctctact acttctaaaa actactgttt ttttcctttg aaatgccttc aaaaagtagg
     8461 atcaggcaga atatactggc agaatcaagg agaagggagc ctaaataagg agaaaaacca
     8521 tagaaaatat aaagtaatta cttataaaac aagcttttta tagaaaaata atatacctga
     8581 gtagggtata aattaagacg gaattccttt ctagattgag atattgacta ggaagaatgt
     8641 taagatttta agcctttaag ctaaaaaaaa accctttttg ttaaataagg ggaatatagc
     8701 ctgtcagttt caaactgcaa gtcgtcctgc tgcaataata tagtagatta gagatgaaaa
     8761 tagatttttt taaaccaatg actgaataaa aagaccgtca tagatacaca ttggaatgtg
     8821 tctattacgg tcttttttta cgaacgattt tattgaagac ggacagttcg ggtcaaaaat
     8881 aaaaaagcta cgatcagaaa taagaataaa atgtatccca aaataatggc tagacggtct
     8941 aacttatttg tcgatagatg tttgtttaga agttgatagg cccataaacc tatcagtcct
     9001 cctgttgtat tagttatcaa atcagtgatg tccgctacgc caataccgaa taggaattga
     9061 atcaattctg ccaagaagct tacaaggaaa ataatcccta attttttaga aaaagaaagt
     9121 ctcttgaaat tgacattcaa aagcacccca aaaggaaaaa agacaatcaa gttataagtg
     9181 gtctcacgta ataccgtcgt attttcaaaa gaaaaagtag aaaaagggac gagattgaca
     9241 ttgctgtaag aataagtgag aaattgtgga agattaaatg atagcttgaa taaaatcaac
     9301 cataatagaa agaataaata aatccctaat aaaaattttg atacataccg catagacatc
     9361 tcctccattt aaaaagtata ccatcgaaaa ccgctgaaaa atcataaaaa agtatgaagt
     9421 ttaaggacca acgtagttat cgtacgaaaa ttagaagctt ttttttgttg ggaatccggt
     9481 ttctttaaca aagctaagat agcgagttgc ttggaaaagt tgctatcttt tttctgtcaa
     9541 aacgactcgg aatgttcgtt ttttgttcta atttctccaa gttgtttact ttttgatact
     9601 aagtttatga aaaatcaaga aatagttgtt atactaggta atgctgacaa acgggattgg
     9661 catttcatct tggttcagct ttttttccaa ttactaggaa ggagggatag attgttcaag
     9721 acttattttc gtttcaataa aatacaagat aatccttttc gtatcatgaa tctgatgcta
     9781 cttatcttta ttgtattatg gttaggttac ataaatcatg attttttgat ttcaagtttt
     9841 tcatcattag ggatctttac ttttttctat tatcaaaaca taccgatgaa atatttgctg
     9901 aaacgtatgt ttctgatcgg ttgcggactg ttggttgctt tcgttttggg gatcttatcg
     9961 acttatgttt tatggcttga accatttgcc gtagctttgg ttgctttttc cagcagattc
    10021 gtgttgcgat tattccatat ttctaaacca ggtggcttgt tttttgcgat gctttctgcc
    10081 atgggaacga gcatgcagct gccaatcgct caattaccga tcgtctcact ttattttttt
    10141 atgggtgtcg tttttgccct gattgcggca gtgattacaa aattactgga ttcacggcct
    10201 gagcagacaa ttgaaaaagc aactttaaaa gaaagatttc acgaagagcc tttagtgatc
    10261 attgattctg ttttctacag cgctgcctta tttctttccg tttatgtcag tcacggcttg
    10321 aatctgcata atccttattg gttgaccttg tcatgtgcat cgattttgct agcagaaaac
    10381 ttggatgcga tgaagcatcg acaagtccaa tatctgattg gatcgatggg tgggttatgt
    10441 gtgtcggcat tcttgtcatt tgtgccgttt acgcagttgc agaccatttt tctgatcact
    10501 tttctatatg gaatcgcaca atttctagtt gctagaaatt atgcagttgc taatattttt
    10561 cttaatccga tggcgttgat gctatctaca cttattcgtg gagcatactt gatttctttg
    10621 atcgaatatc gttttttagg gatcgtcata ggaagtttca tcggtttagg tgttgcgtgg
    10681 gtaatgacgg ttggtttgca acattattta acggttgttc gtcaaaaatt agaatagaag
    10741 gacaaatata tgtgccaaaa gaggttatga caagattttt gtcacaacct ctaattcaag
    10801 gtgttcaaaa gccagattct cagcattaag tcaaattcac acaaaaatgt ggaaaggaac
    10861 tttcaaaaaa attttatatt tattctatca aaaaatttta tatttattct atcaaaaaat
    10921 tttgtttatg atggtaactg aataaacttt cctgagatgg tcaatgcttt ccttgaaagt
    10981 cttacagata ttctttcttc atagaaagtt taagttttat agttagtgta caataaaagt
    11041 atgtttatac catatggtgt ccggataatc agtcatttaa acaatgaggt tcaaagatat
    11101 gaaaagggga aatttgatga agaaaataag caatgaaatc caaattctgg cagaaacttt
    11161 aactcttccc aaagtcgtta tagagcaaat gattgtacaa gcaaaaaaca tccttgatga
    11221 ggtgtttgag caatatagtc atgaactaat ggatcctaat aaagcaaagg cgactgcaaa
    11281 gaaaatagac aaagtctatg aagaggaacc ttttgcagca ttagctattt atctatatgc
    11341 agcttctcaa agttggaaaa aaatttatca accattgaag attccaagaa ctatctattt
    11401 ggcaacaatg aatgctttta ctagatttat tcatgaacat ttccaagtaa caggaagcta
    11461 tcgctttgac cgtggttttt ggatttggca ttacactagt ggcttgatct ttcggattgg
    11521 tgaattggag tacgaaatga catattttcc gaaagggtac acggatctaa gattagaagg
    11581 aaagcagtgc ctttctcttc atattccttc cgatgcagat ttatctcaga ataagataag
    11641 agatagttac gaaagtgcca ttcgtttttt tgaactttat tttcctgatt accattacca
    11701 agtgatgtac acagatactt ggttactatc acccaatctt actaaatggt tgaaaaaaga
    11761 gtcaaaaatc tgtctttttg cagccgatta tcgattatta tcagttgatg aacaggacga
    11821 tagtggagtc ccttggatct ttggacgagt agatgcccaa atacatgatt atccagaaag
    11881 tacgagttta cagcgacaag caaaagagca attgcttgca ggcgagcaca ttggctctgg
    11941 cttaggtatt cttacttcat aagtttagta cttcgaaata gtttaaaaga gcgtgagaca
    12001 aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacggcgga agcagaagca
    12061 actccttcgg aaataagctg aaattcacaa aaatttgaaa agcaattttc gtgaatttct
    12121 tcttatttct tggagttaaa cgcttctgtc ccagcctctt ttttagtgtt ctatcgaaca
    12181 cacagtacgt tcaataggta aactagctat aacaatagat attaaagtta acggacaaga
    12241 gggccttttt taggtagcta aaacgtaata atgaaaattt atttccagag ataggagtca
    12301 atcagctagt ctgatcgact gttttaacaa gttgactttc attttaactt ttgataccat
    12361 ttaaaacaca aatcgtaatt gtttcgattt gtgtttttta gcatgactac aatcaaagaa
    12421 gaaagtgggt cgtccatatg gaaaaaacag atatctcaag tgcataccgc cgcttgaaaa
    12481 gcccaaatat caaaacacga aaacgtgcgc tgaagatcat caaagatgtg aaacggaata
    12541 gtgggaaaag gtaagaagaa ggaaacttaa ttaaaaaaat agccgaaaaa cgtttttcgg
    12601 ctattttcaa taatcggttt ttgtgtaagg tttccgaaag aactgctgtt gattttccat
    12661 ttttaacata tgataaattg gtagaatctt tcttttcaaa ttgataccat tccctaattc
    12721 cagttatata gttaactaca gaaccacaaa gcacgtacgt ttataaaagt attatctgta
    12781 gttatttttc aattcccttt caacctctta ttttgaccag tagtattgat cactttacct
    12841 tttaacacct ttgttcgttt ttgaaacatt tttagtcaag aatggaaacg taactacata
    12901 atcaaaggtt ttgcgaaata tttcatgaaa tatttcatgt agaagcgagc aatacggttg
    12961 caaataacca ttatcgttta ataagcagtt acgaaagctc ccaatatctc taacgttcag
    13021 ctcactccat ttcttttctt caaaaaaatc agtgattcgt atctctttct atttgtcgaa
    13081 aaagttaact ttaaaaagta acgccgaaaa aatatgcaaa aaaataaaaa agcagttact
    13141 attgctagaa taacatcaag gaataataag ttttggtttt cagataatag tcgataagtt
    13201 gtgtattgga attttagata cagtaataaa gtatttttca tgaaattttg tgagaatcac
    13261 tattacaagt atctttaata aaattaatgg ttaaggagga acttaaagta gaatgtaaat
    13321 gaatagttgt agtcagtcga ttgtgatact agactcaaag ttgcaggaat aagtttttca
    13381 atttgtgatt ggaagataaa aaatgaaaaa gaagggcttt tttttatact ttgttgattg
    13441 tagtgtttat tttccctctg tttagccaag taacattttc tatgactcaa ccagtttcaa
    13501 gtgaggaaca tgataacctt tttcgtgcta cagaagagtt taagaaatat caggaaaata
    13561 ttgttccgga gactccatta gctagtgagc caattactga tattgatgga aaccaaatag
    13621 gatggtatat acagtacgaa gttatggtgg taaataatga ctcaatagta aaaaatacag
    13681 taggcttaga gctaacaccg attgaaaagg tgagttcatt gttaacgtat atttatactg
    13741 atgaaagtga cgagactgag atcattttaa cggattactc aaaaattgct actgataaaa
    13801 agtatatatt gtggatgtag acactagtga aatactttat gatatagaaa ttgatgaaaa
    13861 tttactagat gtagcacaag aaatcgatca tcaaaagcag gaagctattc gatatgctgg
    13921 tagaaaaggg aaaagaaaat atttattacg tgaatgtagc aaaagttaga gaagatgaaa
    13981 atgagtggaa agaattcaag agtcgctact caattaactc gactccgaca tttacagttt
    14041 acagagaagg atcgatagaa aaaactgttt tttggactaa agaaagtgga atgtctttag
    14101 ctgaagtaga agagtttttg gattatgtta gtatgcaaca gtaagggagt tcttattatg
    14161 aaaaattttg acacatatta tgttattgtt ttttcgatat ctttgctgtt aatattacta
    14221 actttaatgc agtataaaga gtatccagag tattttattt taacttttgt atatttagta
    14281 agtagctaca cacttctata taaattcaga agttcttcta gagtttctaa agtttgtttc
    14341 ttaattagct tattgattct ataatatttt tgatttatat aggaaattaa aaatattata
    14401 gaatcaacac tcagagaacg ggttggtttt taatagattt aataagtact gataatgaaa
    14461 tttctcctgg ttgggaaaac gaagttggtt accctgtaga agaatttttt caagattact
    14521 ttgcggagta tgccgatagg ataatgagag attacgtaac agataaaact caagaagtca
    14581 tcaatcaatc ccagtcatta aatgaattat ctgataaggc gaaagaagaa gcagttgcca
    14641 ttatttcttc agggattagt gactatattg atatgagtgg tgaaatcaat tatggcgata
    14701 aagacaaaat tgtacgcatg ataaaagctt ttatcaaaac ttttgatgtg aaggcagatc
    14761 ttaagtttaa tgatacttac ctagtaggaa aattaattaa tatccttggg acagagaaag
    14821 atacggaaac gtttatttcc tctttgtcta tgttgtttac agataagcat ttttcaactc
    14881 agcttggtgg agaggaacta gtggaaatct tttaaccatt acgcccagca ttaataaaag
    14941 tatatcaaaa aagtactcct gatgattttt aagattggtt tgagaaataa tgtgaaaaca
    15001 ctatttttaa tgttaattat agatagagat gaacgcgtca tatggttaaa agaatgaaaa
    15061 agtcaaagat acactgctat atttcactaa ggatatagca gtgtatcttt catgtcgttt
    15121 cactggcagt tttttataaa aggaggtgtg catttcagtt gcttttcttt ggtagttagt
    15181 tgaatcgttt aagaattaac ggatgtggca gacaggtgct ctgtccaaaa accagtaaaa
    15241 tctttgatgg ttccatcttt atttagctgt gcctgttcaa atactgtttt gtttttaccg
    15301 aatggatcaa ttgcaataat tgttccgatc cccgcacgtt tggcagcaag taatcctgag
    15361 taagcatctt cgataacaag acaatcagcc ggagctaagc tcagtttgtc tgccgcttta
    15421 agaaaaatat ccggttgcgg tttcccaggg aaagagccgt cgtcatagac aatcttgtcc
    15481 cagtcaaacc agcgggctaa atcgaaaata tcaaaataaa aagcaacatt ctctttgaca
    15541 gtagccgtgg caatcgttaa tggaatctgt tggtcagtca gctggtcaag agtcgcttca
    15601 agcccttttg tcaactgaag ctcgttttca ttttgcaagc aaagcatacg ataatagctt
    15661 tctttttctt ctgacaatac ggcgatctca gaggcggtca aatgctctga aatgaaatgt
    15721 ttcaaaatct ccgtattcgt ccgaccatga atatttttta aaatatcctc gtctgataaa
    15781 ccatcttttg tatgatcatg tatcatatgc agccaagcag cttcatgcag atgagagtcc
    15841 aaaaacattg ttccgttgaa atcaaatata atccctttca tagttacgct cctatagtgg
    15901 tattttcggt tgaatcagca atttgcagat cgacatattg tgcatagtgc tcttgaacct
    15961 cccgcgagat ctggcgatcc gtaatgagca gatcgaaatc atgcaaatta taaaacacat
    16021 aaaaatcatc tcgattgaat tttttattat caacgagtag atatttctta cgggaattat
    16081 ttagagcgat ctgctgtgct tctccttctt cggtactgta agtagagatc gcttcgttgt
    16141 gaacgccatt tgaactgata aatgcttttg taaactttag tcttcttaaa ttgtcgttgg
    16201 tgatcggtcc gacaaatgcg ccggtatttt tccggaaatc accgccaatc agtagaattt
    16261 ctggtccatc tgcttgagca agaatatcaa aaactggata attatttgta atgatccgaa
    16321 ttttcttgtt cagtaaatgc gtcgccaata gttcgattgt tgtacctggt cctaagaaaa
    16381 tcgtttctcc ctcgtgaatc aaggaggctg ctgtggcagc aatttctttt ttttcttgaa
    16441 tctgcagagt ggctttttca gtgtgagaaa gttcttgatc taaagtgtag gaaagacttt
    16501 gagcccctcc atggacacgc agcagcttgc ccgatttttc taattcatct aaatctctgc
    16561 gaacggtcat atcagaaaca tctaattgat tgatgatttc attaacggta aggattcctt
    16621 ttttattgac catttctacg atagtaagca atctttcttt tttcaacatt gtcttcacct
    16681 ctaatttaag gataatctat catataaacg gacaacagtc aatctaattc gaacaaaagt
    16741 aaacatttag ggtggttttt atgtttgtta caatagattt tattgctgtt ttctggtttt
    16801 tatgattatt tgtgtttgtt tttgttgaca aatgttttgg atgcgtttac aatgcaatca
    16861 gttagaaaga gcgaaaactg attatagatt tgaggagaga tgtacatgct taaattacca
    16921 gaagatttta tttttggcgg agccacagca gcctatcagg ttgaaggagc cactaaggaa
    16981 ggtggcaaag gcgctgttgc ttgggatgac tttttagagg aacaagggcg attcagtccc
    17041 gatccggcaa gtgattttta tcatcaatat gcaaaggaca ttgaattgtg tgaacgtttt
    17101 ggtgtaaatg gtctaagatt atccattgct tggagccgca tttttcctga tggagctggg
    17161 aaaccaaatc cagaaggaat cgctttttat catcgtgtgt tcgaagaatg taagaaaaga
    17221 aacgtaacgc cgtttgtgac gttgcatcat tttgataccc ctaaacgttt gtttgaccat
    17281 ggagattttt tgaatcgaga aacaatagaa gcatttgttt cctatgccat tttttgtttt
    17341 catgaattca aagaagtcaa ggtgtggagt acctttaatg aaatctatcc agttgcaacc
    17401 aatcaatatt tgttaggtgt tttcccacca ggaatcaaat atgattttac aaagatcgtc
    17461 gcctgtcttc ataatatgat ggttgctcat gcccgtgtag tgaactattt caaagaaaac
    17521 gaacttccgg gtgaaattgg tgtagttcac tcattagaga cgaaatatgc ggcaacagac
    17581 gcgccggaag ataagcatgc tgccttttta gacgatgcat tgtcgatccg tttcttatta
    17641 gatgctacct atttaggcta ttattcaact gaaaccttga ctgctcttga tgagatatgt
    17701 gaagcaaatc aggccagcta tcattttcct gaagaagatt tcgttgaatt gaaaaaagca
    17761 tccacgcgta atgactactt agggatcaat cactaccaat gccattttgt caaagcttac
    17821 gatggagaaa atgcaatcca tcataacggt acgggtgaaa aagggacctc tgtttataaa
    17881 gtgaagggaa tcggagaacg tatttataaa gaaggaattc ctcgaactga ttgggactgg
    17941 ctgatttatc cagaaggtct atatgacttg ttgctgcgaa ttaaaagcga ttacccacat
    18001 tacaacaaaa tctatatcac tgagaacggg atggggtata aagatcagtt tgaagatggc
    18061 attattatgg atcagccgag aatcgactat ttgagagtct atttggaatc cttaagcaag
    18121 gcgataacag ccggggtaaa tgtgaagggg tattttttgt ggtcgcttat ggatcttttc
    18181 tcatggacaa atgggtataa caaaaggtat ggcttgtttt atgttgattt tgaaacccag
    18241 aagcgttacc caaaagaatc agcttactgg tacaagttgg tcagtgaaac caaaacgatt
    18301 atttaataag tgataatgga ggaagaaaag atgaataaag aggaattaca aatgcttggt
    18361 tttgagatcg ttgcatattc aggcgacgct cgcagtacat tattgaaatt attgaaagaa
    18421 gtaagacaag gaaactttga acatgttgat tcggcgttga aagaagccga cgaaaattta
    18481 acccttgcac ataattcgca aacaaaaatt ttagcggaag aagcatctgg gaaagagatg
    18541 gagatgggat tcatttttat ccatgggcag gatcatttaa tgaccacttt attgctgaga
    18601 gatctgattc aagactttat tgtactgtac agacagaatc aaggatagga gaagaacgct
    18661 atggatggaa tcattaaaca aatcgaaaaa gggaaaccat tttttgaaaa aatctcacgt
    18721 aatagttatt taggagccgt tcgtgacgga tttttaacag cgatgccagc tattttattt
    18781 tcaagcattt tcattttgat tgctgcggtt cctgagattt ttggttacca atggcctgaa
    18841 tctgtctcca catggctatg gaaagtctat aattattcga tgggtgttgt agggttactc
    18901 gtttcagcaa ccactgctcg ctgcttggct gattcgatga atcgtcgctt accgaaaaac
    18961 aaagtaatca acaccacctc tgtgatgctg gcctctgtgg taagtttcct gttgttaagc
    19021 gtctctccga ttgaaggtgg attttcaacg gaatatatgg ggacaaaggg actactggct
    19081 tcctttgtcg ctgcattttt aacagttaat ttatataagt tttgtgtgct gaaagatatt
    19141 accattaaaa tgcctaaaga agttcctgga acgatttcgc aaaccttccg agatattttt
    19201 cctttctctt tttcagtatt tgcggccgtt ttaattgatt taggcattcg acaaatcttc
    19261 caaatctcat ttgcagaagc aatcattacg gtgctgcagc cgctatttac agcagctgac
    19321 gggtacttag gaatcgctat tatctgggga gcgatggcgc tgttttggtt tgtcggtgtt
    19381 catggaccgt cgatcgtgga gccggcaatt gcggcaatta tctatgccaa tgtggaaacc
    19441 aatctgcaat tgttcaattc cggtgaacaa gcttctaaag tactgacggt gggtttaggg
    19501 aattttgtag gaactatggg aggaactggg gcaacattgg ttgttccttt tatcttccta
    19561 ctctttgcta agtctaagca gttgaaggca gttgggaaag cttcctttat tccagtaagt
    19621 ttcgctgtca acgaaccatt gctatttgcg gcacccatga ttttgaaccc ttattttttt
    19681 attccatttt tgttaacacc tgttgtaaac gtttgcctgt tcaagttttt tgtcgatgta
    19741 ttgggcatga acagctttat gtacgttttg ccttgggcaa ctccagcacc gatcggtttg
    19801 gttctaggaa cgggcatggg tatcttagct tttgttctag cagcggttct aatcgtcgtt
    19861 gacttcctga tctatctacc attttgcaaa gcatacgatg ctgtcttagt agagaaagaa
    19921 gcacaaaaag cagaggtgat cgcaaccgag gaaggtgctt ctgccgatgt acgtgaggaa
    19981 gtacctgtgg cagcgagtca agttatcagc gatgcaaata aagaaatcaa tgtgttggta
    20041 ctttgtgctg gcgcagggac aagtgcgatg ttagctaatg ctttaaaaaa aggtgcatct
    20101 gaatcgaaag caccgatcag tgcaatggcg ggtgcttatg gttctcatta cgaaatgctg
    20161 caggattttg atatggtggt cttagcaccg caagtcaatt cttactatga tgatatcaaa
    20221 gaagacaccg atcgcttagg aatcaagctg gttgcgacaa aaggagcaga atatatcgga
    20281 ttaacgagag atcctgaaaa agctgttcag tttattctga accaatttca ataaaggagg
    20341 agccataatg aaaatgggaa tttggttgcg taaagctgtt ttatttgcag ctgtgttagg
    20401 ggctttgggg gctgtttcaa aaactgttga agcatctgcc agcgaattta ttcgcggagc
    20461 agatatttcg atcctgtcag atatggaaaa atctggtgca aaatattatg aagacgatat
    20521 ttcaaaagat gccttagaga ttttaaaaaa taatggtgtc aactatgttc gactgcgttt
    20581 gtggcaggat ccttataata gtaatggaga aagttatgga gcagggacaa atgatctgga
    20641 aacgacgatt gaccttgcaa aacgagcaaa aaatctggga cttaaggtgc tgctggattt
    20701 tcactacagt gatttttggg ttgatcctgg gaaacagaat cttcctaaag catggcaagg
    20761 tctgaccttt gaagaaatga atacggcatt gtacgactat acaaagaacg ttctgtcgga
    20821 aatgaagcag ctggacgttt atcctgatat ggttcagatc ggcaatgaac tgaatagcgg
    20881 aatgctctgg ccttatggca agtcttgggg agaaggcggg ggagaatttg atcgattggc
    20941 tgcttttttg aagtctggta tccaagctgt tcgagacact caacctaaaa caactccagt
    21001 aatgctccac ttagctgatg gtggcgatac aggagcattt acttggtggt ttgatgaaat
    21061 cacgagtcga ggtgtgtcat ttgatttaat tggagtgtcg tattatccgt attggcacgg
    21121 ttcaatgaat gaacttcaaa ccaatatgaa tgcgatcagc caacggtatg gtaaagaagt
    21181 gatcgtagtt gaaacagctt atgggcatac aacagcgaat gccgatacta tgccaaatgc
    21241 ttttggtgaa gccgaagcag ctgcgggggg atatgaacca acaccgacag gacaagcaga
    21301 gtatttgctg gatcttgccg atcggattca ggcagtaccg aataatcgcg gggcgggctt
    21361 cttctattgg gaacctcttt ggtacaacgg aaatgtgagt tgggcaacac aagcaggtat
    21421 gaattatctc agtgttcaat ccactatggg aaacgaatgg gacaaccaag cgatgtttga
    21481 ttttgctgga aacgccttgc caagcttgcg tgcgtttaaa caagcagggg cgcaaataaa
    21541 tctagttaaa aatgcaagtt tcgaaagcga tggaccaacg accagtcctt ccaattggca
    21601 aacctggttt caacaaggaa ccgctaaggg tacgatcaaa acggaagcgg gcactttttt
    21661 tggggactac aaactaactt tttggcagga tcaagcatac gaggcatcag cttatcaaaa
    21721 tgttagtggc ataagcaaag gaacttacac attgagtgcc tatgtaatgt ctgggggaga
    21781 gtttgatact aatcagttat atatcaaaaa ttatggtgga gcggaactaa atactgcggt
    21841 tgttaaaaca agtgaatgga caaaaattac aatcaaagac attccaatta caacaggtgt
    21901 ttgtgaaatt ggggtttata gcaaggcttc aggaaatgct tggacgaacg ttgattttgt
    21961 ttcattggtg aaacaataga ctgcggatcg tttcgattca agatcggagg ttcccttcag
    22021 ccacatcgtt agcgtagaag gcaggaatcg aacctgcacc tcgcctttgc caaaaataac
    22081 cagaaacgat ttccagtcat ttttaccaaa acgagtaaag caccagactt catctacact
    22141 ttgtattatg ctaaagagag ttcaacgatt tcgtaagaag caatgaaaaa attgcaaaaa
    22201 gttgtgcaga taacataaag tttgtttaag aaagggtaac cttagaattc cgtcgtgaat
    22261 atgatttgag cacttagatg gatagaaaaa agttctcgga aaacagttcg tttttcgaga
    22321 acttcttttt atgataagaa tgaaacaaat taacttatat tatagtctct tttggtaggg
    22381 aaccatctta tttttaatga aaatcaagca ttttagctat tgaagaatgt tgatttatag
    22441 ggattccccc tccatattat gttggctctt tattcccact ctgttttcaa cgtaaagtgt
    22501 ataaaacaga atgcgatact atcaagggag ttaaaacaat atacagaaca gtaaaacact
    22561 ataaataaaa ataaaagtgc aatttttgca cctaatcata ctctgtttat atatgaaaac
    22621 tagattctga atccaatatt tggttaacga gaggcgttcg aatttgaacg tcttttatat
    22681 attactttca aataaatatg aggtagaata ctatctattt tctttttaac ccaattgtta
    22741 taat
//
LOCUS       DAIGOK010000036        22625 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.036, whole genome shotgun sequence.
ACCESSION   DAIGOK010000036 DAIGOK010000000
VERSION     DAIGOK010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.036"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            133..3660
                     /locus_tag="NOA01_001106"
     CDS             133..3660
                     /locus_tag="NOA01_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HCI0632316.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="NOA01_001107"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="NOA01_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HCI0632317.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQVIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="NOA01_001108"
     CDS             7448..7789
                     /locus_tag="NOA01_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCI0632318.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="NOA01_001109"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="NOA01_001109"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCI0632319.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="NOA01_001110"
     CDS             9146..11566
                     /locus_tag="NOA01_001110"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCI0632320.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="NOA01_001111"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="NOA01_001111"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCI0632321.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="NOA01_001112"
     CDS             13000..13737
                     /locus_tag="NOA01_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCI0632322.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="NOA01_001113"
     CDS             13751..14566
                     /locus_tag="NOA01_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCI0632323.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="NOA01_001114"
     CDS             14579..15226
                     /locus_tag="NOA01_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCI0632324.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="NOA01_001115"
     CDS             15242..15898
                     /locus_tag="NOA01_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCI0632325.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="NOA01_001116"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="NOA01_001116"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCI0632326.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="NOA01_001117"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="NOA01_001117"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCI0632327.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="NOA01_001118"
     CDS             18240..18758
                     /locus_tag="NOA01_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCI0632328.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="NOA01_001119"
     CDS             18865..19365
                     /locus_tag="NOA01_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCI0632329.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="NOA01_001120"
     CDS             complement(19645..20565)
                     /locus_tag="NOA01_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HCI0632330.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="NOA01_001121"
     CDS             20730..21743
                     /locus_tag="NOA01_001121"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HCI0632331.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="NOA01_001122"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="NOA01_001122"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCI0632332.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
BASE COUNT         7530 a         3947 c         4821 g         6327 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagaa
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagtcatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtggacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtt
//
LOCUS       DAIGOK010000037        21956 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.037, whole genome shotgun sequence.
ACCESSION   DAIGOK010000037 DAIGOK010000000
VERSION     DAIGOK010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.037"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..260
                     /locus_tag="NOA01_001123"
     CDS             <1..260
                     /locus_tag="NOA01_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCI0632333.1"
                     /translation="AAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNP
                     GNKGAIAFHQLIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            505..1530
                     /locus_tag="NOA01_001124"
     CDS             505..1530
                     /locus_tag="NOA01_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCI0632334.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLRIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            1824..2933
                     /locus_tag="NOA01_001125"
     CDS             1824..2933
                     /locus_tag="NOA01_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCI0632335.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASANTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            3016..3912
                     /locus_tag="NOA01_001126"
                     /pseudo
     CDS             3016..3912
                     /locus_tag="NOA01_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            3928..4752
                     /locus_tag="NOA01_001127"
     CDS             3928..4752
                     /locus_tag="NOA01_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCI0632336.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINIFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGGKL"
     gene            4784..6094
                     /locus_tag="NOA01_001128"
     CDS             4784..6094
                     /locus_tag="NOA01_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HCI0632337.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSRAYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            6242..7501
                     /locus_tag="NOA01_001129"
     CDS             6242..7501
                     /locus_tag="NOA01_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCI0632338.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            7511..8383
                     /locus_tag="NOA01_001130"
     CDS             7511..8383
                     /locus_tag="NOA01_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCI0632339.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            8395..9225
                     /locus_tag="NOA01_001131"
     CDS             8395..9225
                     /locus_tag="NOA01_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCI0632340.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            9265..11448
                     /locus_tag="NOA01_001132"
     CDS             9265..11448
                     /locus_tag="NOA01_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCI0632341.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLQERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            11509..12729
                     /locus_tag="NOA01_001133"
     CDS             11509..12729
                     /locus_tag="NOA01_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HCI0632342.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKYSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVCLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            12726..14186
                     /gene="gtfA"
                     /locus_tag="NOA01_001134"
     CDS             12726..14186
                     /gene="gtfA"
                     /locus_tag="NOA01_001134"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HCI0632343.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVTQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            14263..15369
                     /locus_tag="NOA01_001135"
                     /pseudo
     CDS             14263..15369
                     /locus_tag="NOA01_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            15691..15900
                     /locus_tag="NOA01_001136"
     CDS             15691..15900
                     /locus_tag="NOA01_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HCI0632344.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            15893..16042
                     /locus_tag="NOA01_001137"
     CDS             15893..16042
                     /locus_tag="NOA01_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCI0632345.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            16196..16330
                     /locus_tag="NOA01_001138"
     CDS             16196..16330
                     /locus_tag="NOA01_001138"
                     /inference="COORDINATES: protein motif:HMM:NF026010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632346.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     "
     gene            16490..16861
                     /locus_tag="NOA01_001139"
     CDS             16490..16861
                     /locus_tag="NOA01_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCI0632347.1"
                     /translation="MTVMRLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPE
                     TASSIRTIELDDNTITFLKAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIV
                     GVHIIRIHGLRHSHASLLISQ"
     gene            complement(17145..18230)
                     /gene="xerS"
                     /locus_tag="NOA01_001140"
     CDS             complement(17145..18230)
                     /gene="xerS"
                     /locus_tag="NOA01_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HCI0632348.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            18564..18797
                     /locus_tag="NOA01_001141"
     CDS             18564..18797
                     /locus_tag="NOA01_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HCI0632349.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            18794..19201
                     /locus_tag="NOA01_001142"
     CDS             18794..19201
                     /locus_tag="NOA01_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HCI0632350.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(20338..20856)
                     /locus_tag="NOA01_001143"
     CDS             complement(20338..20856)
                     /locus_tag="NOA01_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HCI0632351.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         7469 a         3359 c         4118 g         7010 t
ORIGIN      
        1 tagcggctaa attagtaaaa tttttaaaaa ataagaataa acgagtgatg gaacaattat
       61 cgattattgg atttgatgat attacttttg cagaattaat ggatcctgcg ttgacaacaa
      121 tacgactaaa tccaggtaac aagggggcga ttgcttttca ccaactgatt ggactggcca
      181 aacaagaaga aataaaaaat caagtactac tattagaggg agaattgatt tctcgagact
      241 ctgttcacaa aatcaaataa aaaagctctg tgaaataagt atagttaaca ataaaatatc
      301 cgaacgatag agagaatgaa ctgtgatcta gcagttcctc gtcggaaatt cactagaatt
      361 agcattattt actctaaatt gttcggatat tttttcttgt ttttaattat gattacagtg
      421 cctattttta acgaaatttg cttattttca aaatgtatga ttatatttta gtaaacactt
      481 tcaaatatga aaagaggagc tattatggta ggaattaggg atgttgcaaa acgggcgggt
      541 gtgtccattg caacagtatc gcgcgtgcta aatgaggatc cgacactttc agtaaccgat
      601 gaaaccaaac gaaagattgc agaatcggtt aatttttatc attatgtcaa aaaaagtccc
      661 gttgcaaaaa atttaacaaa tattgcatta ataacgacag tttctgaagt agatgagttg
      721 gaagatccct attttcgaac gattcgcaga gggattcaag tcgaagcaga acaaagcaaa
      781 gtcaatttga agaaaattat tcgtttgtca gaaacaccgt tggagcaaga tgatttgcta
      841 aaatgtcaag gtgtgttgat tattgggcag gtgctatctt ctgtgattga agaagtatca
      901 gcaattaatt cgaatgtcgt agtgattgat gaccctagta ttgaagctga agtagatgca
      961 gtttatactg atttaacaaa agcaacgaat cagcatttag atcgattgta taaaaaagga
     1021 caccgaaaca ttgcgtttat cggaggagga cgtgttcgtt tagatagcca aggaaataaa
     1081 cacgtgtcag atgatgatta tcgaaaaata gcctatgaga cgtggatgaa acaaaaagga
     1141 ttagaaaaat atagtcaaat ttatttagaa ggctggacca cacttgacgg aatgcaggca
     1201 gcagatttac ttttgaaaaa acatgctaaa gataagttgc cgactgctat catggtagga
     1261 aatgatccta ttgctgtggg ggtttatcgt tcacttcaaa aaaatggtct acgtattcca
     1321 gaagatattt cgattgtgag ttttgataat attgaagttg ctgaattttt aacacctgca
     1381 ttaaatacgg tgaatgtgaa tacagaagaa attggaaaac tagcagttcg catggttaat
     1441 gaacgaataa aagaagttag aaatgtttcg atcaaagtga ttgtttctaa taatattatt
     1501 gttagagaaa gtgaaaaaaa tatcgattaa aaaagtaaac atccaaaaaa ggatgtttac
     1561 ttttttgttt actaatattt ttagtaaaaa atctcgacaa aacattaagt aaacagttat
     1621 ggtgttgtta acaaaaagaa aacgcattca aaacaggagg aattaagatg aaacatttca
     1681 aaaaaatagc tttaacaggg atagctgttt taactgggac attactcgca gcatgtggtg
     1741 ggggagatag taattctaaa gacgggcaag taactttaag ctatgcgagt tgagatactg
     1801 accaagctcc aggactaaga aaaatattag atgaatttga aaaacaaaat cctgatatca
     1861 aagtagaaat ggagacaacg ccttgggatc aatattgggt taaattagaa gctgcaacaa
     1921 cgggaggtaa tatgccagat gtagtaacca tgcactctag cgaatcctat aaatatatgt
     1981 cacagaatgc tttgatggga cttgatgatg ttatcaaaga aaatgatatt gacatgggaa
     2041 actttactga aggaattgct gatttctata cgcttgaaga cgaactatac gcaatcccta
     2101 aagatgcttc tgtagtgggg ctatggtaca acaaagaatt atttgataat gcaggtgtcg
     2161 attatccaga tgaaacatgg acatgggaaa cactcagaca agctgcaatt gatttaacag
     2221 atgaatcaaa aggcatctat ggttttgcat cacatattgg aacagagact ggctactggc
     2281 catttattta tcaaaatggg ggagaagtat ttgccgatga caatacaaaa tcaggtttga
     2341 ataccccaga agcacaagaa gctttagctt tttatcgaga tattgcactt gttgatcatg
     2401 cttcaccaac aattacagaa ttacaagatc ctgataagtt aagtcgtttt caatcaggta
     2461 gattagcaat gatgattgaa ggaaactggt ttgcaccggg atttttgaaa aatgactatc
     2521 tagttgaaaa tgttgcttct gccgtgttac ctaagggaaa agttaatgca acaattacaa
     2581 atggacttgg ttgggctgcc agtgcgaata caaaataccc agaagaagtg aaaaaactaa
     2641 tggcattttt agctagtgag gacgccaata aaatccaagc ggaaacgggg gcatcaatcc
     2701 cagcactaaa aggctttgaa gacgagtggg ctgcttcttc agatacaatt gatttgagtg
     2761 cttttggaaa aatgttggag tatggagtcc ctcgtccata caataaagat ggattacgag
     2821 ctgaacaagc agaagttcaa attattaacc gtattctgtc tggtgaagta actgttgaag
     2881 atggaaccgc tcaaattgaa aaagaagtca atgcgatatt gagcggaaat taaattagtt
     2941 aattatacaa tatacaggcg aaaggattct atttcgtctg tatattaatg gatgaatacc
     3001 aaaaaggggg atattatgaa aaatcgaatt tttggaaaat atcgtaaaga tcaagtgata
     3061 tggggctata tttttatttt tccaacggca ttaggattaa ttttattaaa tatatggcca
     3121 gcaattcaaa cagcaatttt aagttttcaa aaaacggtag ggttcggtga tactgaatgg
     3181 gtcggattaa gaaattacca aaaaatgttt gcagacggtg aagtttttca gtctttatta
     3241 aacacattaa tttatgcagt ggtttcgata cctagtattg ttgtattatc cttgttggtg
     3301 gcagttttaa tgaatagaaa agttaaagga ttgtcgattt atcggacaat ttattttcta
     3361 ccagtcgttg ctgcaccatc cgcagtcgca atgatttggc gctggatgtt caataatgat
     3421 tatggactga ttaataacat gttggctaaa ataggtctcg atggaccagc atggttaacg
     3481 gatccatcga ttgcgattta ttctattatc attgtaggag tttggagtgc tattggttat
     3541 aacatggtgt tattactagc cggcttataa gaaattccta aagattatta tgaagcagcg
     3601 gaaattgatg gtgctagtcc aatccagcag tttttcaaca ttacgttacc attagttaca
     3661 ccaacattat tttttgtagt tgtaacaaca gttatcaatg ctttccaagt atttgatgtt
     3721 atttttatga tgattactcc taatagtacc gccatgttta aatcacaatc attagcctat
     3781 ttattctata aacattcatt tattttaaat gacaagggat atggttcggc aatcgttatg
     3841 ttccttttag tgattatatt aattattact gcaattcaaa tgaagttgca gaaaaagtgg
     3901 gtaaactatt aaggaggcta tcaaattatg aaaaataaat ttttttcaaa tgcgccaatc
     3961 catatcatgc ttattttagg tggtattgca atggcattac cttttatttg gatgatttta
     4021 acttcaggga aaacgttggt tgaatctacc caaattccac ctacaatttt ccctgagaaa
     4081 tttcagctaa gtaactatca acaagtactt gatttattac cattcatggc tttttatatc
     4141 aatatatttt taatggttgt tggacgagtg attggctcgg ttttattttc agcaatggct
     4201 gcatatgctt gtgcgcgtct aagttttcca gggaaaaata tccttttcag cttagtactt
     4261 attcaaatga tgattcccgg ttccattttt attactccac agtatttatt agcacaaaaa
     4321 ttgggtattt taaatacaat tccagcatta atattaccag gggtagttag tgcgtttggt
     4381 acgtttttat taagacaatt tttcttaggt ttgcctgatg aattggagga agcggcctat
     4441 ttagaaggtg ccaatatttg gacgatcttt ttcaaaatta tgttgccgct tgctaaatca
     4501 ggattggttg cacttggaat attcacagcg ttgtttgcat ttaaagattt aatgtggcca
     4561 ttgattgtca atacttcaat tgataagatg cctttagctc cgggattagc aaacttacaa
     4621 ggacaatatg ccacgaactt cccacaatta atggcaggtt cgttattagc aatttggcca
     4681 atagttgtga tattcatcgt tttccaaaga aaattcattg aaggaattgc tacaacaggt
     4741 ggtaaattat aaaactatta attagtgatt ggagagtaag tacatgacaa aaattacatt
     4801 tattggtgca ggaagtacta tttttgcaaa aaacgtatta ggcgatgcga tgttaactcc
     4861 aagtctacaa gatgcacata ttgcattgta cgatattgat gaaaaacgac taaaagaatc
     4921 aaatttaatg cttaaaaata ttaataaaaa ttcgaatgaa aatcgtgctg atattgtttc
     4981 ttattatgat cgcaaagaag cgttaagaga ggctgatttt gttatcaatg ctattcaagt
     5041 gggtggttat aaaccttcaa cagtgattga ttttgatatt cctaaaaaat atgggttgcg
     5101 acaaactatt ggtgacacca tggggattgg tgggattttt agaaccctac ggacactacc
     5161 agtaatgtta gactttgcta aagaaatgga agaagtttgt ccaaatgctt ggttattgaa
     5221 ctatacaaat ccgatggcga tgttaaccat gggtgtttta aaagcgacga gtattaagac
     5281 tgttggttta tgtcatagcg ttcaagtatg cgtaccagaa ttgtttgagc atttaggaat
     5341 agccgatcaa tatgacttaa atgactttca atggaaaatt gcaggcatta atcatatggc
     5401 attcttgttg gaaattacaa aaaatgggga agatttctat ccaacaattc gtcagttagc
     5461 tgcagaaaaa ccaaatccac atccagattc tgtacgtttt gaattgttga aacgttttgg
     5521 ttactatatt acagaatcaa gtgaacacaa tgcagaatac catccatact ttattaaatc
     5581 aaaataccca gaattgattg acgaattagg gattccgttg gatgaatact tacgtcgttg
     5641 tgaacaacaa attaatgatt gggaaagcat gcgagaggac attgtaaata atggggagtt
     5701 aacacatacg agaagccgtg catatgcttc atatatcatg gattctatta cgacaggaac
     5761 tccgtcagtg attgcaggaa atgtcttaaa taaaggctta atcacaaact taccgacaga
     5821 tgtttgtgtg gaagtacctt gtttagtcga taaaaatggc gtgcaaccaa cctatgtcgg
     5881 tgaattgcca acacaattag ctgctttaaa tcggacgaat atcaatgttc aagaactgac
     5941 agtagaagcc gctttaacac tagataaatc aaaaatttat caagcagcgt atttagatcc
     6001 acatctatca tctgaattgt ctttaacaga tatcaaaaat ttagtggatg atttaatcga
     6061 agcacatggg gactatttac ctgaatataa ttaaaaagaa gctgaaagag taaggaattt
     6121 tcaattcctt actcttttct tagttaaatt tagtaaaaat attgacaaaa cacttagtaa
     6181 acagttacat tagtgttaac aaaaagaaaa cgcattcaaa ttgtataagg aggagcataa
     6241 aatgaagtgg tctaaaaaaa ttattttagc ttgtatagca ctaggagcaa cgatctcttt
     6301 gactgcttgt ggaaaaaatc aggaagatgc aagcggtaag gtagaaatag agttttttaa
     6361 tcaaaaaaaa gaaatgactc aaacgattca ggagattgct aaagattttg aagcaaaaaa
     6421 ccctgatatt catgtaaaag ttgtggatgt accgaatgct ggtgaagtaa ttaaaacacg
     6481 tatgcttgca ggcgatgtac cagatgtcat taatctttat cctcaaagta tagaattgaa
     6541 agaatgggcg aaagctggtt atttagagga cttaaccgaa gaaccgtatt tagagaatat
     6601 caaaaatgga tatgcacaac gttttgctat tgaggatcgt gtatatagca taccattaac
     6661 cgctaatgtc tatggttttt actataacaa aactgctttt gaggaaatgg ggattcaagc
     6721 accagaaact tgggcagaat ttgaaaaaat agtagctgag attaaagatc aaaacaaagt
     6781 accgtttgcg attgcaggag cagaaggctg gactttaaat ggttatcacc aactagctat
     6841 agcgactgct gctggtggtg aagaggaagc gaacaatgtt tggcgtttct cagaagttaa
     6901 tggaattaat gctgaaagca aagaaatgca aaaagatttt aatcgattag atttgttaag
     6961 agaaccaaaa gctttgcaaa acaactggca aggtgccggt tataacgata cagtagttac
     7021 ttttacaaaa ggtgatgcgt tgattatgcc gaatggttca tgggcaatgc cgatgattca
     7081 ttcacaaaat ccagattttg aagtaggaac ttttcctttt ccagcagatg aagcaggtca
     7141 aagtctaacg attggcgcag gagacctagc cttatcagtt tctgcaactt ctcaacataa
     7201 agaagaagcc aaacgttttg tagaatatat gacaacacct gaagcgatgc agaaatatta
     7261 tgatgtagat ggtgcaccgt gtgctgttga gggcgttatc gagaatactc aggattcacc
     7321 attaagtggt ttaactgaac ttgcttttac tgatcgtcat ttagtttggt tagccaaaga
     7381 ttggaatagt gaaaatgatt tttatacctt aacaacaaat tatttacaca atggcaatca
     7441 aaaagccatg attaacgcat taaatgcttt ctttaatcca atgaaagctg acggaaggta
     7501 ggaaaataaa atgaaaataa aagaaatttt aaatcgttac tgggcaatca tttttgtagc
     7561 aattccaatt attttgcaga tgattttttt ctactttcca atggttcaag gtgcttttta
     7621 cagtttaact aattggactg gtctgactta taattttgat tttgtgggtt tgaacaatta
     7681 taagattctt ttttctgatc cgaagtttat caaatcgatt ggcttcactt taattgtgac
     7741 gctttgcatg attgctggac aaattggttt tggtattttg attgcgcggg cgttgaacgc
     7801 aaatattaaa gggaaaacat tctttagagc atggttcttt tttccagcgg tcttatcggg
     7861 cgtggttgtt tctttagtat ttaaacaaat ttttaactat ggtttaccaa aaattggtag
     7921 tatgttaggc attgaatttt tacaaaccag tcttttagga acaacatggg gagcgatttt
     7981 tgcagtaatt tttgttttac tttggcaagg aattgccatg ccagtcatca ttttcttagc
     8041 gggttttcaa agtattccac aagaaattat tgaagctgct agaatggatg gggcgaatga
     8101 taagcaaatt ttttggaata ttgaaatcac ctacttatta ccaagtattt caatggtatt
     8161 tattatggca ttaaaaggtg gcttaacagc atttgatcaa atttttgctt taactggtgg
     8221 tggaccaagc gatgccacaa catctttggg gttacttgtt tataattatg cttttaaaaa
     8281 caatcagttt ggttatgcga atgctattgc tttagtctta tttatcttaa ttgggattgt
     8341 ttcactggca caattaaaat tatcgaaaaa atttgaaatc taggaggttt cccaatgaaa
     8401 acatcaaaag aaaaaaatat ttctaaatac ttgcttttat tattaggggg cttcatgatt
     8461 ttagttccct tagtttcaac gctattcggt tcatttaaaa caacacccga tattatgaat
     8521 aatttctttc gttttcctaa tccagcgact ttggaaaatt tccgaagatt gttagcggat
     8581 ggaattggtc attacttttg gaattcggca atcatcacaa tcattgcagt agttgctgtg
     8641 acagcaatta ttcctatggc tgcttattca ctagcaagaa aaatgactcg tgggaaagca
     8701 tacggattga tgtatttatt tttaatttta ggaatttttg ttcctttcca agtaattatg
     8761 attccgatta ccgttatgat gagtaaaatt ggtttagcaa atatgtgggg attaatctta
     8821 ctctatctca cttatgccgt accacagaca ctatttttat atgttggttt tattaaacta
     8881 aatgtgccta tcagtctgga tgaggctgct gaaattgatg gggctagtca ctttacaact
     8941 tatttcaaaa ttgttttccc aatgttaaaa ccaatgcatg cgacgactat tattcttaat
     9001 gctctatggt tttggaatga tttcatgcta ccattattaa ttttaaatcg tgattctaat
     9061 atgtggacac taccattgtt tcaatataat tatacgggac aatattttaa cgactatggt
     9121 ccaagttttg cttcctatac agtaggaatt gtcacgatta cactagttta tcttgctttc
     9181 caaagaagta ttatagctgg aatgagcaat ggagcagtga aataaccaat aaattattct
     9241 cattataaag gagttcataa acttatgatt atatttgaca atcacacaca aacatttcat
     9301 ttacaaaata aaaaaattag ctatctatta ggaatcgaag aaaataatta tttaacgcat
     9361 ctttattttg gaaaaaagat tacgcactat tctggtgggt atcgctaccc ccgaacagat
     9421 cgttccttct cacccaacaa tgcaaatgcg caagatcgtt tattatcttt agatacatta
     9481 ccattagaat ttccaggata tggacatggt gattttagag aaccggcgca taatattaaa
     9541 ttaggaaacg gtagtcgtgt aaatgatttt cgttatgata gctacgaaat tcaaaaagga
     9601 aagcccagct tagcgggatt gccagcctta tatacggaag atgatgatga agctgaaacg
     9661 ctgattattc aattgaaaga cgcgattagt ggattgactt tacagttgtt ctacacaatt
     9721 tatcaaaatc acagtgtaat tattcgttct acgcaattag taaataatag tgatgaaaca
     9781 gtggagatta accgcttagc tagtcaatca attgattttc caaatcgtcc actcgagtta
     9841 attcatttaa atggtacctg ggcaagagaa cgtcaaatga ctagagaaaa aattcataca
     9901 gggataaaag tattagatag taaacgcgga tcaagcagcc atcaacaaaa tccgtttgtt
     9961 gctttagtag atgccaaaac agatgaattt caaggagagg catatggatt taatttagtt
    10021 tatagcggta atcacgaaac ggtgattcaa aaagatccat ttgaccaaac acgggtcatt
    10081 atgggaatta attcatttaa ttttggttgg caactaaaaa ctggcgactc atttcaaacg
    10141 ccagaagtcg tgatggttta tagtaatgaa ggtctgaata cgttatcaca gacgtatcat
    10201 gaattgtata atcaccgttt aattcgtgga aactatcgtt tacaagaacg tcccacgtta
    10261 atcaacaact gggaagcaac gtattttgat tttaacgaag aaaaaatcat gaatattgtc
    10321 gatgatgcga agaacttggg cattgaaatg tttgttttag atgatggttg gtttggcggt
    10381 cgtgaaaatg acttcacttc attaggtgat tgggttgaaa cagaaggcaa acttgcttgt
    10441 ggtttggaaa atttagccaa gcaagtgcat gataacggga tgaaatttgg gatttggttt
    10501 gaaccggaaa tgatttctga agatagtgaa ctatttcgag cacatccaga ttgggcattg
    10561 ggtattccag gtcgtgaccg ctcaattagt cgaaatcaat atgttcttga tttttcaaga
    10621 caagatgttc gtgataatat ctatcaacaa atgacggcga tttttgatcg tgtaccgatt
    10681 gattatgtga agtgggatat gaatcgaaat atgacagaag tctattctgt cttgttggac
    10741 tcaacgatgc aaggtgaagt ggctcatcgt tatatgttag ggttatatga atttttagaa
    10801 aagttaacaa cgaattatcc aactatttta tttgaaagtt gttcgggtgg aggcggtcgt
    10861 tttgatgcag gtttcttgta ttacatgcca caaacttgga cgagcgacaa taccgatgcc
    10921 gtggctcgtc tgaaaattca atatggaacc agcttagtgt atccaatttc aagtatgggt
    10981 gcgcatgttt cagcagtccc aaatcatcaa acaggaagag aaacgagttt aactattcgt
    11041 ggcaatgctg cgatggctgg tgtttttggt tatgagctag atctaacgca attaacttct
    11101 gaagaaaaag cagaaattaa agaacaagtt gccttttata aaaagcatcg ccaacttttg
    11161 caatatggaa ctttttatcg cttggaaagt ccgtttgaag gcaatgatac tgcatggatg
    11221 tttgtctcaa aagaccaaac agaagccatg gtcttttatt tccgagtatt agcagaagct
    11281 gcgcatccat tagtcacatt gaaattagca ggattagcag aagaagcaac ttatcaaatg
    11341 gaagatgtaa catttgctgg cgatgaattg atgaatctcg gtttttatat tgactcagaa
    11401 ctacatggtg attatgcgac acaacgcttt tatttgaaac agatatagta gcttgaattt
    11461 acaatataag tgcaacacca aaagagactc attccaaacg aagaaaatat gttttattta
    11521 cacgcattta ctaaaaagca gcctgatttg aattgggaaa atgaagaagt acgtgaagaa
    11581 atctatgcaa tgattaacta ttggttagat aaaggactag gcggatttag aatcgatgcg
    11641 atattaaaca tcaaaaaaaa actagagtat ggcatctttg aatctgatgg agaagatggg
    11701 ttagccttca ttgggccctg gattcttaat cagtcgggca ttgaagtgtg gttaaaagaa
    11761 atgagagagc ggacatttaa accacataat agtatgacgg tagcagaagc cgatgttcct
    11821 aatgaacgtt tagatgaata tattggtgaa gatggctatt attcgatggt ttttgatttt
    11881 agttatacgg atattgatgt acctgaaact ggtgaatggt ttaaatatag ccaatggaca
    11941 tggacggata tgcgtaccaa tatttttacg aatcaattag ttacgcaaga taaaggttgg
    12001 ggcgccttat atttagaaaa ccacgatcaa ccacgttcaa ttaataaata tattccagaa
    12061 gaatggatca atgattatag taaaaaaatg ttggcgacgc tttttatgtt actaagaggt
    12121 acccctttta tttatcaagg acaagaacta ggaatgacca acatagaaat ggcatcatta
    12181 gaagattttg atgatgtagc tacgcattct cagtataaac gggcattaga atatggattg
    12241 gcaccagagc aagcattgaa agctgtcaat aaacgaagtc gggataattc tcgcacacct
    12301 atgcaatgga atacgcaaaa aaatgccaga ttctcaactt ctgataatgt atgtttaaaa
    12361 gtaaatccta attatccaga actcaatgca gaagctcagt taaccaatca acagtcggta
    12421 ttcaattatt atcatcaatt aattaaatta agaaaaaata gtgtatacaa agacgtgcta
    12481 atctacggac aatttctacc agtgaaagat aaaaatgaac atattttgct ttatgaacgc
    12541 caattagaag ataaaatcgt attagttatc ttaaacgtta cagcaacaga acaagaatat
    12601 tatgtagatg aacagtacca acaagtatta atcaataatt atgaagatgt tttattagat
    12661 caaagtcgaa aattgcgttt acgaccgttt gaaagtattg tattagctaa ttatggagga
    12721 caattatgaa aattaaaaac gaagcaatgc tcatcaccta tgcagatagt ttaggaaaaa
    12781 acatgggcga attaaaacaa gtcttagaca aacatttaca aggtgttatt ggtggtgtac
    12841 atctgttgcc tttttttcca tcgacaggtg atcgaggctt tgcgccgagt gattatacga
    12901 cagttgatcc agctcttgga acatgggaag atgtggaagc attaggtgaa gattattatt
    12961 tgatgtttga ttttatgatt aatcacattt ctcgtgaatc aaaatttttc caagatttca
    13021 aaaaaaacca tgagcagtca ccatacaaag aaatgtttat ccgtattcat gaatttttcc
    13081 cagaaaatcg accaacacaa gaagatattg atttaatcta caagcgtaaa gacaaagcgc
    13141 cgtttcaaac ggtagaattt gcggatggta cgacagaaga ggtttggaat acctttgggg
    13201 aagaacaaat tgatttagat gtgaccaaag aagttgtgaa agaatttatt cgagaaacca
    13261 tcaaagatat ggcgaaccat ggctgttctt taattcgatt agatgcgttt gcttatgcga
    13321 ttaaaaaatt agataccaat gatttcttcg tggaaccaga tatttgggaa cttttagacg
    13381 aagtgcgtca agaagcagcg aaatatgacg tcgaattatt accagaaatc catgagcatt
    13441 attccattca aatgaaaatt gccaatcatg attattatat ctatgacttc gccttgccaa
    13501 tggtcactct gtattcactt tatagtggaa aatcagaacg tttagccaat tggttgaaaa
    13561 tgagtccgat gaagcagttt acaacattgg atacgcatga tggaattggt gttgtggacg
    13621 ctcgtgattt attaacagat gaagaattgg attacacctc tgaggaattg tataaagtcg
    13681 gggcgaatgt gaagaaggtc tattcaagtg ccaattacaa taatttagat atttatcaaa
    13741 ttaacagcac ctattatagt gcattaggcg ataatgaccg tagctatctg ttagcacgtg
    13801 cgattcaagt atttgcgcca gggattccac aaatttatta cgttgggtta ttagcaggaa
    13861 aaaatgatat tgaactgttg gaagcaacga aagaagggcg taatattaat cgtcattact
    13921 atgatttagc cgaaattgaa gaagaagtgc aacggccagt gactcaacaa ttatttgcac
    13981 ttttaaaatt tagaaatgaa tctgcggcgt ttgatttgga tggaacgatt gaagtagcaa
    14041 cgccatcaga atcagaaata gtgattacac gtaaaaataa aaacggtagt gatagtgcaa
    14101 cattaacggc taacttggat acgaaagaat ttatcattga gtctaaagat ataaaaattt
    14161 tcactagtgt tgcattaaaa ttttaaccta ttccatagaa caaaaaatcc tttatactag
    14221 cacttggatg acttatccaa gaccaataaa aaggactcat acatggataa gtataaaaca
    14281 aatacagcat ttaataaatg gttttctgcc ataaaattag gaaaactttc tgtcaactcc
    14341 caaaaatgta tttttgatta caattcgtat cgtaaaaaac tgtcttttga agctgtgctc
    14401 aagctctttc tctacgcagt taatgaagaa aaagaaagtt tgcgtgattt gagtacagct
    14461 cttatcgacc gatcgctcca actagaaacc gatgtgtcat cgattagtca tacccaattg
    14521 tctcgagcat ttaatgcact tgaacctaaa gtgttagaag aaatttttca acaactgtta
    14581 gaaaaagttc aacatcaaat caaaccaacc aagcgaaatc atctgtatct gattgattcc
    14641 agtacgtttt cgcttagttt aaaacgtcat aagtgggcga ctttccgtca aacaaaatca
    14701 ggtgtcaagt tacacttaaa ctattgttat atggacgata ctacgatgta tccaactgat
    14761 tttaccttaa caaatgccca tgaacatgat ctcaatcaac taccagtatt ggtgaatcaa
    14821 ccagaggcaa cttacgtgtt tgatcgtggg tattaggact ttgaaaagat ggatcagatg
    14881 cattgggatg gctatttctt cgtcactcgg attaaaaaaa acaccaaagt tcacgtgata
    14941 gatactctgg aaacatcgcc agaaacagaa attctacgag acgagctggt tcgtttagga
    15001 tctaaaacct atctaaccgc caatttccga ctagtcactg ttcaagataa gaacggacgt
    15061 gtgtttcaat ttatcaccaa tcgaatggac gtctcttcaa aagaaatctc tgatatgtat
    15121 cacgctcgtt ggcaaatcga attgtttttc aagcacatca aacaacacat gaccattaaa
    15181 accttctttt ctcaaagtga aaaaggcgtg cagaatcaat tgattttaac gatgatttcc
    15241 gctctcctca cgtttctcat caaattggag acgaaaacgg aaaaatcagt gttccaaatc
    15301 aaacgattct ttcgttattt actgtttcag ccctttgaat gttgtctaga aaaattaatt
    15361 cccacatgag aaaaaagaaa tttggctcgt tacttttggt tttcagttga ataacagata
    15421 aaaaattttc tggaaaaagt catcctctgc gtcagcacag ttttttttga cttattttca
    15481 gaggaaatag ttttcagaat attctaaaat gttttatgca acactagtga tattttttag
    15541 aattagattt ctaatgtgca ttttttgagt attaaggagc aagtatacaa agatttataa
    15601 agctgattaa atttgaataa aaatatgcag gaacgctact atttttagta gtgttctttt
    15661 ttatatttga ataaaaaatg tggtggttaa atggtggtta taacaaaaaa tgaattaatg
    15721 aaattaggtt atgggaagta tcagtctgag agaatcatac gacaggcaaa agcaattatg
    15781 gtttcaaaaa gctataccta ttacgaaaat agaagattag gactagtacc tgttgaatct
    15841 gtagaagaag ttttaggtat gaagatagag gtagaggagg attttcagaa tcatggttaa
    15901 ttttaaaaaa gataaaaatg ggaaatatca ttttactgct agtttgggat tcgacaaacg
    15961 aacagggaaa agagtacaaa aaaacgtaga ggggttaaaa cagttaaaga agaaaaaaag
    16021 cgtataccga acttgtttct aattatggaa aagaagcaga aacttctcaa agtactatgt
    16081 tgtttgagga gtttttttat aaattattcc taccttatta tcaagggaaa gtagaagaaa
    16141 gaacatataa aaatcggtta tctacaatca aaaaaacttt tcttatttct ttaaaatgga
    16201 gttaagagaa atacgtccaa ttgacattca aaagtggcaa aataatttat tgagcaaata
    16261 taaaaattct tatgtaagaa acatgtatgg gatttttcag atgtcattag atcgagcagt
    16321 tgttttgtga ctacttgaaa aaaatcctgc aaaagttatt ggaaatgtga agaaaaggaa
    16381 aactacagtt gatttttgga caaaagaaga atttacaaaa gttatttcaa ctttttatat
    16441 tgatgatttg taccaacatt ttggttttat ttgtatgtgg ttacttttta tgaccgttat
    16501 gcgtttggga gaagccactg cattaacttg gaaagatatt gattttgaaa ataaaaactt
    16561 gcgtgtgaat aaaacactct attataagag agccaaaacc tatgagtttg tagaacccga
    16621 aacggcttct agtattcgta caatcgaact agacgacaat acaattacct ttttaaaagc
    16681 ttggaaggag cgtcaagttt caataggagg catgtcgttt gtactttctt ataatggaac
    16741 acctactcag aaatatacaa tccgtcatat tatgagaaga catgcaacga tcgttggtgt
    16801 tcatataatc cgcatacacg gattaaggca tagtcatgct tctttgctta tatcacaata
    16861 aaaagatgca cttcgaatta aagaaaggtt aaaacatgct gatgtacaaa cgactttagg
    16921 aacttatggt catttgtatc taaattcatt gagagagata acaaatgatc ccacagatta
    16981 tatccaaact aatttttcta aaatcaaacc gacacctatt tacgataata aaaaataaat
    17041 gataaacaaa agtaggatca caccaaaaga aacacactag aaaaactata atacaggctt
    17101 ttctagcgtg ttgtttctta tttccgccta tttatacatt atactcataa agcatttaat
    17161 gcatcgatcg tagccgcatt gtctatatgg gtatataaag ttgttgtatt ggtacttgaa
    17221 tgtcccaatt gttgggcagt taataccaat gaatttgtct gttggtagag tctagtagct
    17281 aatgtatgac gcagtttgtg agggctgact tgaattctaa agccttttga atattttgct
    17341 accatccgtt cgattgcaat accggatata cgcttggctt ctccttggta aattgctaga
    17401 aataaagctt tttcgttttc tgttacttta tatcgttctt tccgaatagc tagataatta
    17461 gctaaatatt ccattgcgaa tgctgctata ttgacagcat ctcttttatt tccttttcta
    17521 gttactacca cattattctt atctaaagat aggtcatcta aattaatatt tataacctcg
    17581 gagagtctca atccagatcc taaaatcagt gcattgatcg ccaggtctct ttctacatct
    17641 cgttgataat agatcaaagc tcgtttactc aaagtatttt tatattcatt ggcaatataa
    17701 tctagataat tttgtgtatc cttatctaga aataattttt tctcaatggc attggcacgt
    17761 gcagctaacg tagttttatc tgtgatggtt gctacttgat ccataggatt attgtctaag
    17821 tatggttttc ctagaggatt attggaactt ttcgaaagat atttaaatag aacctttaat
    17881 gccgtgatat ttctattgat agtagaagta gatagaccta agttattatt atctttgagc
    17941 ttagtttttg ttgtctcttt ttttacattt tcttttgtgc gacgtttcaa atgagatttg
    18001 tacaagttta ggtcataggc agttaactgt cttatcgagg tgatagaagt atcaggaacg
    18061 atagactcac tcagaagcca ttcataaaag agcttataat tttttagata ttctaaagtg
    18121 gttcgaggtg attgatcttt atcctcacga tcttgaacaa aatcttttat ataatcgggc
    18181 atatgaagta tgattgtatc tattttttga tatatttctg tatttttcat gtcattcatc
    18241 ctttcaataa gtaggggaga agagtatctc tataattgga ctataccaat tatagaatgt
    18301 tttgtcaatt ggtatgctct aaaatttatt aatttgtttc aaaaaaagca tataaacagg
    18361 tatacgttta ataatttaaa ataaatggta tacataaata gggaaggaaa tagaagggag
    18421 caatctaaat tttttgaatt ttcttaatta attatataga aagaacaatc tttctataga
    18481 gttagacatg agagggacta tgcttgattt aaaatcaaca agcgtatata ctgtatatac
    18541 aaagaaaaag gagggagaag actatggcaa ttaaagaaag aaaatttagg gaagtgggga
    18601 actcagtagt attaactatt tcaaaggagt ttcttaaaga aaatgggtta aatcccggag
    18661 atacggtttt tttagatgaa gaaaaacttc gagcggcaat tacaaaaaaa caagccttat
    18721 ctgatgaaat tgatttagca atatctcaag cattaactga atatgatact acttttagag
    18781 agcttgtcga taaatgagta ttcattacct agaagccaaa gatattataa aaatgaatgt
    18841 aatccaaata aaaaaatact ctcctaatga accccttggt gtaaaggatg cgaatgcttt
    18901 agaaatgtgt gtagggcaac ttcgagcctc agcttttggt gaagaggttt atccttcgat
    18961 ttatgaaaaa gcagctatcc tacttataca attgataaag aaacatcctt ttcacaatgc
    19021 aaataaacgt actgctttct tagctacttt tgtctttttg aaaatcaatg gacagctttt
    19081 agctattgat caaaaagatg ctgtaaattt agcggtatat attgctacgt atgataagga
    19141 tttcgatcaa ctaaaatatg aagtgatcaa cactataaag acacatacgc agcccattta
    19201 aggagttact gtatgtgtct ttatagtgtt attagctgct atagcgaaat aattgaagta
    19261 aattacaaat agctataatt caaatccaag tatatagaaa acgtaaattt tctatatagt
    19321 tggattaaga taacgactat ttattttatc atatttttac tgtaccctat acttttactt
    19381 ggtatattta tgaataatcg caaatttacc cctatactac tagtttgtaa gttagattaa
    19441 taaaaatact aaagttattt gttcttttat taaatacacc tacaacttat aaattgatgg
    19501 tatttagata acgcgaaaga ataaaaataa cagctgatag taaagtttgt tgacgattgc
    19561 tactcaatcg aaagtgagtt agtgcgtatg aacttgcttc tgaaaactta tgaaatgaaa
    19621 gaccttttac atcttgcaga aatcgttact gggattaaac taagcaataa aaactaatgt
    19681 tcccctcatt ctttgaactt ctactatttg atttatttat tctgataaat gacgatttca
    19741 ccttcaggat cttcaaatcc cgtatagaat ttatttaaga tctcttcagt atcagtaata
    19801 ggataggcga taggccacat accctttgat ggttagaata aactgctcaa aatctgtatc
    19861 gtatgttggc tttagcatat taacaaaggc tatctccaaa ttataaacca taagcgttga
    19921 atcgttctta acaatttcaa ttcttcctaa cccaaagtca gaagatataa ttaccggact
    19981 tgccttaccc ttcatgtggg ttgttgagat tccatattta aagggaattg tcggtactat
    20041 cggcggtctg aaaaacatcc caaaaataaa aaaatctttc ccctgctagt ctcctaagtc
    20101 cctttgcagc ggatatggca ccataatctc attttatcga agtgagtcat ggtttatctg
    20161 ttggccaacc aaaattgata caatctaacg atgcaccctc tacggcattc taggggagtg
    20221 cacttgcatt cacccaataa aaaccggcag ttagcaaaga acttatgtag tcgtatgttc
    20281 ttactgttaa catacaagag gtctcctttc cacaattgct tagcagtaaa cgggaattta
    20341 caaagctaat ctcttccatt ttcttatctt tgttctaaaa gtcccaaatg gagcaactgt
    20401 atttacatga ataaatttat atacttccca tacagccgtt ttggttgcct cgtctgccca
    20461 ttttcttttg tgtgcagtga atagatcttc atcactcatt gaatcaatca tcatgttaat
    20521 atcatcaata ttacgattga gaatttcctc taattctttc aatggtagat gtgcataggt
    20581 atttgtaaac cacttatata actctcctaa ctgattccat ttaaattgat cagaaggcgt
    20641 tttaacagat aatccatttc tttcatcatt ttcccatttt agaattaaat tcgtccaacc
    20701 aacttgatat gctaaatttt cagcaggtgt tctatccact tcctcaactc ttttatcctt
    20761 caattcttca ggaatagttt caaattctaa aatatattta gtataagtcc tatttatttc
    20821 tttttttaat tcttctttat ttttatatgt tctcattata ttacctccat aaattcctaa
    20881 ttgtctcctt taattcatct ataatttcat ccacgatatt ttaatccgtt ctacaatgca
    20941 tatctgatat gcattgactg tctaactcat atgtgaatga tttataaaat atcccaacgt
    21001 ctcatctaaa gaaatgttga tcgtcagcaa ggagctaaaa aatacgaata tatttctttt
    21061 gtagtaaaat cggactggtt tgcatatcat tttcttcgta aatatcaaaa tcatgttaac
    21121 tactatcaat tactaataaa aactaccact ttaagcgtta agaactgtta agtttagtta
    21181 agtgaggata gttcacaaaa tccattgtta aacttcacga aagtggtaaa actcaaaatg
    21241 ctttatatag gattacagca tcgctctttc ttcgattgcg cgttgggtaa aaaatattat
    21301 gaagtaaaaa ttgacgatga cacgttcctt acggctcatc agatgaaaca gctacaaaaa
    21361 cgaaatgatc aactcgaaga aaatttaatt ctaaaaaagc gattgccata ttcacgcctc
    21421 gctcgacgaa ctattaaagg tcaagattta cctgaagatc atccttttta ggaaattgaa
    21481 gctttttatg ttgcacaatg catttataat actacaatga tgttttcacc agatattctt
    21541 ctagggtgcg gtgtaatgaa acagatacac ttgcaaaaga aaattcaccg cgaatttact
    21601 aaaattatga ataattatgt taatcttccg aaattttttt ccaaaattaa gggatcaagc
    21661 tggtgttata gattgtttaa ggttggcaaa aaaataactt ctttttaaac aattatagaa
    21721 aaccgatact ctgttaagtt ggttgacacg gtatcgattt ttatgacttt cttattctga
    21781 aacttcacta acggacagta cctaaaaagt ctatagtatg gacgtttacg agtactattt
    21841 tatcaaaaat gctgctcatt tcaacttata tgttttagtg aactaccccc acttagctaa
    21901 acctgacggt tcttaacgct tgaagtggga gcttcttggg aacagagcgt acttga
//
LOCUS       DAIGOK010000038        21910 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.038, whole genome shotgun sequence.
ACCESSION   DAIGOK010000038 DAIGOK010000000
VERSION     DAIGOK010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21910
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.038"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            422..493
                     /locus_tag="NOA01_001144"
     tRNA            422..493
                     /locus_tag="NOA01_001144"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:455..457,aa:Lys,seq:ttt)
     gene            633..800
                     /locus_tag="NOA01_001145"
     CDS             633..800
                     /locus_tag="NOA01_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632352.1"
                     /translation="MYRPQYLEQKHEVITVQNGNGEIVRKYRRPIKSNTYKRKESNEV
                     IPLYGKRIAKY"
     gene            826..1170
                     /locus_tag="NOA01_001146"
     CDS             826..1170
                     /locus_tag="NOA01_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCI0632353.1"
                     /translation="MTEEFYRWLLQLIREDCLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            1175..1456
                     /locus_tag="NOA01_001147"
     CDS             1175..1456
                     /locus_tag="NOA01_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632354.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNIFGYSIEEINYYIDFAEAFIPMVR"
     gene            1559..1873
                     /locus_tag="NOA01_001148"
     CDS             1559..1873
                     /locus_tag="NOA01_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCI0632355.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            1851..3545
                     /locus_tag="NOA01_001149"
     CDS             1851..3545
                     /locus_tag="NOA01_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCI0632356.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            3565..4743
                     /locus_tag="NOA01_001150"
     CDS             3565..4743
                     /locus_tag="NOA01_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCI0632357.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKDFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            4706..5392
                     /locus_tag="NOA01_001151"
     CDS             4706..5392
                     /locus_tag="NOA01_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCI0632358.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            5392..6552
                     /locus_tag="NOA01_001152"
     CDS             5392..6552
                     /locus_tag="NOA01_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCI0632359.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            6562..7437
                     /locus_tag="NOA01_001153"
     CDS             6562..7437
                     /locus_tag="NOA01_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632360.1"
                     /translation="MKYILCQLAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            7434..7745
                     /locus_tag="NOA01_001154"
     CDS             7434..7745
                     /locus_tag="NOA01_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632361.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            7735..8088
                     /locus_tag="NOA01_001155"
     CDS             7735..8088
                     /locus_tag="NOA01_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCI0632362.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            8078..8479
                     /locus_tag="NOA01_001156"
     CDS             8078..8479
                     /locus_tag="NOA01_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632363.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            8472..8876
                     /locus_tag="NOA01_001157"
     CDS             8472..8876
                     /locus_tag="NOA01_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632364.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            8888..9496
                     /locus_tag="NOA01_001158"
     CDS             8888..9496
                     /locus_tag="NOA01_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCI0632365.1"
                     /translation="MAKTKTVVTTFDNISIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELEAKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            9516..9878
                     /locus_tag="NOA01_001159"
     CDS             9516..9878
                     /locus_tag="NOA01_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632366.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            9881..10063
                     /locus_tag="NOA01_001160"
     CDS             9881..10063
                     /locus_tag="NOA01_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632367.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            10080..13499
                     /locus_tag="NOA01_001161"
     CDS             10080..13499
                     /locus_tag="NOA01_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCI0632368.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNDIDKTSKALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFIDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            13550..14287
                     /locus_tag="NOA01_001162"
     CDS             13550..14287
                     /locus_tag="NOA01_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCI0632369.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFTLNDYVFSFEGLVVGTDIVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            14297..16588
                     /locus_tag="NOA01_001163"
     CDS             14297..16588
                     /locus_tag="NOA01_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCI0632370.1"
                     /translation="MIDNLLTIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREVA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNVGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            16612..18789
                     /locus_tag="NOA01_001164"
     CDS             16612..18789
                     /locus_tag="NOA01_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632371.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPYLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAETKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGGYDYSGNPNLMANINADSFS
                     QGSGALSVVDDGDEVVITLDPNHKLEVLKPKSQPALLTGKTYTVSVEIMLEGDFTGDP
                     SKIGLRYIKMPNWVSELYTRNTLTATKGVWQKLTGTVKITAASDNAESWLIMLQNKDA
                     NNSLSGKLRLRHAKLEEGSTATPYQPNLLAEPYNMCREYPNENIADPKVKFPIKSGDH
                     QIYQGYMEEDLMIGQTYTITLKGTKPASQTFVAYNHWTVRLGELKPVDGLTDVWSLTF
                     TPTNVAAMPKLFRVYQYPQSTVGACQIDWLKIEKGNTRTPNISEYKYRGTGMRDSNNP
                     KDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDA
                     QTKANANATAIDERINDTATTAFYGAQGEVSNKTKVAEYGVGTGYTTTAVGETHFERQ
                     SNGYVKCLKAGKYSISAQIRVQLGGKYASWLYTDLYKDGTSVDNLVSYGVDALQNRFA
                     ASGNIVTDLEVNDVLNMRTEIGVADGILQFTSCRTLVLTRLSD"
     gene            18867..19109
                     /locus_tag="NOA01_001165"
     CDS             18867..19109
                     /locus_tag="NOA01_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin XhlA family protein"
                     /protein_id="HCI0632372.1"
                     /translation="MKDEAIQDVVERLVRIETKLDNYELLREKAESAKDRADQAYSIA
                     LNNAEDIKEMKANNKWSWGYMIGLGVTIIGYFLTKL"
     gene            19125..19322
                     /locus_tag="NOA01_001166"
     CDS             19125..19322
                     /locus_tag="NOA01_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCI0632373.1"
                     /translation="MILPDKYYQVIKWAVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            19333..20352
                     /locus_tag="NOA01_001167"
     CDS             19333..20352
                     /locus_tag="NOA01_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HCI0632374.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHNKATFEKNYKAYVELARDSAKKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYGTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
     gene            complement(20392..21731)
                     /locus_tag="NOA01_001168"
     CDS             complement(join(20392..21290,21290..21731))
                     /locus_tag="NOA01_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HCI0632375.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT         7600 a         3333 c         4530 g         6447 t
ORIGIN      
        1 aagagaaatg attccattaa taatttcaat ttttgcgctc tgtcttaatg tctatatgat
       61 tggatttaaa aatgggcaaa ataaaaaata gtagcagcca agaataattt tatagtgtca
      121 ctgtggcgga aagggtagac gctaagcatg tgtgctaggt caatgcttcg gcaaccatgc
      181 aaggttcgat tccttgccag tgactttagt ggatatccaa ggtaaaagga acggaagtgg
      241 gaatcaggca tgattcggta ggataactgc gagggcatat taagttgacc acttaagtaa
      301 aataattagg gagatgcacc tagaaataga gtcggtcgct ctatagggta gtcatcaaat
      361 tagactttgc tatggttaga ccctaagcca gtaaagttgt ggcggtgaac ccttgccgct
      421 ttgacgtgaa gtatctgtgg tgatacgtct gacttttaat cagaaggtgc aggttcgact
      481 cctgtcacgt caattagcaa ccgagggtgg tatgaactcg tgtggtgcga gccctaggga
      541 ggaacaggat aaccgcctgt gtgtaggttg ctagtacata ttagatcact cgttgagtgg
      601 tctttttatt ttgcaaaaag gaggtaacaa caatgtatag accacaatac ttagaacaga
      661 agcatgaagt aatcactgtg caaaacggta acggtgagat agtacgaaag tatagaagac
      721 caataaagag caatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca
      781 aaagaatagc taagtattaa ataagattgc gaaaggagat gggacatgac cgaggaattc
      841 tatagatggt tattacagtt gataagagaa gattgtttgg ttaagttcta tcagtctcct
      901 aaatggcgca ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt
      961 agaagactag gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat
     1021 agacctgact tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat
     1081 gaagttcatg gcagatatct tacagcatta gataaacaag agaagaagat agaaagcttt
     1141 gctaacttcg atgcaagtga aaggtggtaa gtgcatgatc atcaatgaca atggcagaga
     1201 gtatgacaca gaaaagattg aagagtattc atcttatact caaggattaa ttaaacgttt
     1261 gatatacgtt cgctatgtag gtattaggga tctgttatca gataactgtt gcagtaaata
     1321 caaagtgaat caagtaagag aagcgttgaa taaagataat aacgttgaaa gaataaaaaa
     1381 tatttttgga tatagcattg aagagattaa ttattacatt gacttcgctg aagctttcat
     1441 tccgatggtg agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac
     1501 ggagggggct gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat
     1561 gtttaaaaac gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag
     1621 ccaattggaa agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat
     1681 cagtttatgg gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact
     1741 ggagaatggt aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg
     1801 attgatgttg gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat
     1861 tcctgagtta taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg
     1921 tatgcaaaga gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag
     1981 atatttactt cgataagcag atggtagagg attcaatcaa tataccagca caatactttc
     2041 cattcgaatt aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga
     2101 aaaaagataa aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta
     2161 aaactggttt tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa
     2221 attatgatat tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg
     2281 tatttaaagt aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg
     2341 aagttattca aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa
     2401 caaaagatgg gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt
     2461 attcaatgat aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt
     2521 atgatacaac taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa
     2581 aaatgattct ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt
     2641 ttccatttat ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa
     2701 aagcttgtcc aactattaat tacaatgcag atttaaaacg gaaaatgttt caagaatact
     2761 ctcaaatgca acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac
     2821 ctatggaaga tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa
     2881 aagaatttcc tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag
     2941 attttgccag cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata
     3001 cttttatcca cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt
     3061 ctatagatca aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg
     3121 taggttggtt tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt
     3181 accgtgcaaa aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc
     3241 gaagcggatc tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc
     3301 aacgtttatt ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag
     3361 agcatatttc taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg
     3421 atggcttttt ttcattcctt catggtttga attttttaga tgatatttat gattctgctc
     3481 ctgtaacagt cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat
     3541 tctaacttga aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca
     3601 aaaaaagcaa cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat
     3661 cgaaaatttg cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa
     3721 ttcaagtcat atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta
     3781 gctcctaata agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata
     3841 ttctacaatg aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg
     3901 gatagttggg atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt
     3961 aacaacatag tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca
     4021 gatcaacaaa ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct
     4081 agtgccatga atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta
     4141 ttccgaccgc aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt
     4201 aaggatttta tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca
     4261 ttagaagatt tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac
     4321 gacatctttg agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac
     4381 atgagtgggt tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct
     4441 gaacttattc aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg
     4501 aattttgtac gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac
     4561 gctattgatg taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt
     4621 ggaaatgatc gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa
     4681 caagtatcga aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa
     4741 tgaaggcgca gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga
     4801 agggaatact gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat
     4861 tgaagtagta attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt
     4921 acttaagtca aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc
     4981 ttcaattatc gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat
     5041 tcaccgcgct tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca
     5101 actggaatca attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga
     5161 tgaagcattg caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag
     5221 ttatggattg gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa
     5281 agaacaaagc gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc
     5341 tgcatttaca gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt
     5401 aaaaatttaa aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt
     5461 aacgaagaat cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt
     5521 ttagaagaag caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt
     5581 gtattaacta cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca
     5641 ggggtcgaag aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa
     5701 tctcatccac tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt
     5761 gtgtcacgtg gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt
     5821 ttagataatg gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct
     5881 gtatgtaagg caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt
     5941 ttagtagaat cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat
     6001 atgccagtcg gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa
     6061 aaagcagaac ctattacagc tttagatgct gtaactatgg gcggtttgat ggcgcgacta
     6121 tcaaaattca atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat
     6181 gtggtcctaa ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta
     6241 ctaactgcta atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca
     6301 acggctgtgc cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta
     6361 ggatctacac taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt
     6421 tatctagcta aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta
     6481 gatattagcg ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca
     6541 acacaagcgt aggtgatcag aatgaagtat attctttgtc agctggcaat caatcggttt
     6601 aaatgggagc ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta
     6661 ttgcttttca gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt
     6721 gaagttcatg tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca
     6781 tatttatggt ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat
     6841 atcgattcgg atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat
     6901 gatgtttggt attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt
     6961 gaaaacggag aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct
     7021 ttggaaacta taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg
     7081 aattattgga aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa
     7141 aaaacaaata tacaaatctg gacagccgag atgtgggcac agctttggaa catgatgtat
     7201 ttcaatattg gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata
     7261 gaaaaagtta aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat
     7321 ttatttttca aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta
     7381 aacaagaaaa aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc
     7441 ctgaacaagt gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga
     7501 acgaagaaga tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta
     7561 aagtgagtac aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa
     7621 gatgtagata cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt
     7681 tattaggttt cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg
     7741 aataatcgta gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact
     7801 accaaaagaa atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta
     7861 aaatttagaa atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg
     7921 aaattaacta agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat
     7981 caatatttta tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat
     8041 ttttatatct atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga
     8101 aaaagaacgt ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg
     8161 cctcaaactc tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt
     8221 cattctcgaa acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa
     8281 tctatatcta actttctatt cagaaaatag agaagattta acaggagact cactagatat
     8341 tatttcattg attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa
     8401 actagagaac caagatcgct atatcgatca attggtattt acgtttagac gattattgaa
     8461 gagtgattgt catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc
     8521 ttgtgcggat ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat
     8581 caaaaggttc ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag
     8641 atttaagaag agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta
     8701 atttgggttt catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg
     8761 ggataggtac ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg
     8821 cacttgatcc aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg
     8881 aacaacaatg gctaaaacaa aaactgtagt aacaacgttc gataacataa gtatcaaacg
     8941 aattgctttt aattttaaga acgcaggaaa tgcaatcgca acgaattgta acggacaatt
     9001 agatggcgaa acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc
     9061 aaaatctaaa ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta
     9121 tcgacgtttc aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg
     9181 tcctgattcc gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga
     9241 aaatagaaag ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat
     9301 tgaaaatggt gcggatgaag tagctgcgtt agaactagaa gcaaaagtta tgcaagatga
     9361 atttggtaaa ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca
     9421 atggatgacg aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc
     9481 cactcaatca cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg
     9541 aactaaacga tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga
     9601 tgatcaatag agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca
     9661 tggatatttc tgttattcaa ggtgcgaagg ctgtgtatat tgcttaccgc caagcgaaca
     9721 tgactgatta tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg
     9781 ccagctatat ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat
     9841 ttgaaaaagc aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga
     9901 aacgtgggtc gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt
     9961 tttaagcgat atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga
    10021 aggatggaaa aactatgcaa taaaccaatc ccagaaaaac taaagaaagg aggtaaaaaa
    10081 tggctaaaaa gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat
    10141 ctacgttaaa agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac
    10201 aagctcaatt acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt
    10261 tagaaaagca gtatgaatta caaagtcaaa aagttgaagt aactagtcaa cgtttagcca
    10321 atgccaaaaa atattatgga gaaaattcca ccgaagttca gaaacttgaa agagaactga
    10381 ttaatcaaca aacagctcag caaaggttat ctaatgatat agataaaacg agtaaggcat
    10441 tggctcaagc aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg
    10501 aacaaaaaaa tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag
    10561 taactgctgg tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt
    10621 ctcaacaatc tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta
    10681 cacagtctga gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg
    10741 atgctgaacg tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact
    10801 tggacgatat cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa
    10861 gcgacattgg cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg
    10921 taggtagttc ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag
    10981 aattaacgaa tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt
    11041 ccacagatgc attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac
    11101 aaaatattac ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa
    11161 cgttacgtgg tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg
    11221 atttaatggt ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata
    11281 tggcagaata tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa
    11341 tgcttcaaaa tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta
    11401 aggaaatggg aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg
    11461 aaagtactag aaaagctttt gaagagtgga aaaatggcgg aggaacacaa aaagacgtta
    11521 ttaattccat gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag
    11581 cttctacaat ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga
    11641 ctgatgttaa ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagact
    11701 ctactactcc gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc
    11761 ctataggcaa cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg
    11821 ctgatgcgtt taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat
    11881 tgactgctgc atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc
    11941 ctactacatt aataatagga gcagttatta ctgctattgc tggagttata gcaattataa
    12001 aaaactgggg tgcaattact gactggttta gtggtttatg gaaaaaattt actgattggt
    12061 tgggtgatac ttgggaaagt ataaaagacg gagcctcatc agtttgggat ggggttaaag
    12121 aaacctggtc tggatttgta gattgggttc aagatatttg gcaaggcgtt tctgattggt
    12181 ttggagagtt atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag
    12241 agacttggca aacattcatt gattgggttt caaatatttg gaacggagtc aaagaagtat
    12301 ggtcgattat ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt
    12361 cattgattca agctggtatt aatattatcg tgggtatttt tgatgtagct ggacagttat
    12421 taggcgcagc ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg
    12481 atactatgac acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta
    12541 tatttgatcc agtagtggca tggtggaatg gtatatggac atctattagt actacggctt
    12601 caaatatttg gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata
    12661 caatcactag cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat
    12721 ctagttggtt aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta
    12781 gcgtgactag cagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt
    12841 ggaatagtat atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga
    12901 atctttggaa tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa
    12961 tttcaagcgg ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa
    13021 gcacgatagc taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga
    13081 ttagagataa aatcagtagt gtctttgata atttgaatat ttcgatacca catattccgt
    13141 taccacattt taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg
    13201 gtgttgattg gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtcggtg
    13261 aagcaggacc tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggggatc
    13321 gtatcttgag tagcacctca gtttcatcta gggcacaaac gattcaacct gtgaacaatt
    13381 acgaattcaa tttcacaatt gatggtaacg cagatgaggt tactatgaag caaacaactc
    13441 aacaaatcat tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa
    13501 caggagagta tttctcctgt ttttttagtt ttaaaaagga tgtgaaaaaa tgactgattg
    13561 tatacattct ataatcgatg gatttcctga ttatttgcat aaattggctt tagcggaaag
    13621 accaaccata ccttctccaa aaagacagag agttgaaact tctgttttag gacggctagg
    13681 tggcttagta caagattact cgtttgaaga catgtcgttt acattgcact ataactattt
    13741 agaagatgtg gaagaccatc aagcattcaa gcaatcgttt tatatcatgc gtcattggtt
    13801 aaactatgca aagaaattag aattctctga tgatcccaac gtctattatg ttatccagac
    13861 tatcgatatt ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat
    13921 cactgcgaaa ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca
    13981 gtcatttaac ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc
    14041 ttcagctact tcatgccaat tcaccttaaa tgattatgtg tttagttttg aaggcttagt
    14101 agtgggaact gacatagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga
    14161 cggagatatt ttagatcggt ccaacaaaat gaagaccatg caatatccaa cgttgcaagt
    14221 ggatattaat cattttaatt gtactggttt gagcaaaata caaatttatc gtaatgggtt
    14281 aaggtaggtg aaatagatga tcgataattt actaactatt tacgataaaa atgacgcgaa
    14341 taatttagct gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac
    14401 agctactgtt agcaataaac taaacggagc cgagatattt cagggtactt attcaataaa
    14461 cggaactaat gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa
    14521 tcgagcaaaa cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat
    14581 agaagtaaaa gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga
    14641 cagcggaaca gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ttttagcgaa
    14701 accagctatc ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa
    14761 gatagaaaag gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg
    14821 atttcatggt gagtttctaa aagataataa cacattacgt catgaaaaaa gtctaggtac
    14881 ggatcataaa atcaaagcga tttatactaa aaacttaact ggtcttgact tagagataga
    14941 tgctcaaagc gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga
    15001 cgagatcact ctaccagaag aagttatttt cacggattac gtggatgatt atcctgctgg
    15061 atatgtttct tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagt
    15121 tgctaaagac tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat
    15181 tgaattagta ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt
    15241 ttcgatgggt gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag
    15301 aatcgtggaa tatacgttta atgttttaac taattcatac gataaattag ttgtaggaaa
    15361 cgtcaaaaca aacttcttag aaaatacaga gaataatgtc agcaatttga ttaatgatgc
    15421 cattgatcaa ttgaaaaatg ttggcgaaat cagtgattta atcaatgata ttgtagatca
    15481 tcaaactgat atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc
    15541 gcctagtcgt attttgatta tggacacacc agataagaat accgcaagga atgttttaca
    15601 aatcaacaat gctggtattg gtttttctaa aactggcatt aatggaacat atgaaacggc
    15661 atggacgtta gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat
    15721 tactattaga ggtactacat taattagtga tggtgctgat tatagaacaa gtattgctaa
    15781 tggcaaaatg acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg
    15841 tgattatgta agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt
    15901 catgattaga aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag
    15961 accgtttcta tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc
    16021 tggcgacggt agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt
    16081 agctggtagg actatgaaat ttactagtga tggtatgcta acgttaccag gttgtttttt
    16141 tggttcatgg gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa
    16201 agattttact gttagaggta ctaaaaactc aactgtacca acagaacatt atggacaacg
    16261 actattgaac gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac
    16321 aggtgacgat ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct
    16381 aagtcggtat atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga
    16441 agatattgac catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt
    16501 agtggcacac cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag
    16561 cacagtgctt gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc
    16621 agtttatata atttggcttt agatttcagc aaagaattaa actacaccaa agctattatg
    16681 gctcgtcagg gtgataaagg gattacggtg acggttaaac catatttaaa tggcttgcag
    16741 atggatacga gtggcggaac atttacttta aaaggaacaa caccatctaa ccgttacgta
    16801 gataatgttg caactagcgt aactagtgaa gaagtcacgt tttctcttga tggcacattt
    16861 atgagtgaag caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt
    16921 ctaacaacgc aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggtcaa
    16981 gccgatgaat atgtttcgca attagaagag ttgattcgaa agtataatga aacttttgat
    17041 gcttttatgg ctgaaactaa aggtagagtg gatagcttaa atcaacagat tactgattta
    17101 actggtcaag ctaaaacgct acaagacaag ttagatgctc tgaaagaaga aatttctaag
    17161 ttaggtaact tacaagtgat gtacagtaac agcattgatt tcgggggcta tgattattcg
    17221 gggaatccga atttgatggc taatataaac gctgatagct tctcgcaagg tagtggtgcc
    17281 ttatctgttg tagatgatgg tgacgaggta gtgattacgc ttgatccaaa ccataaatta
    17341 gaggtattaa aaccgaaaag tcaaccagcc ctattaacag gcaaaacata taccgtgagt
    17401 gtagaaatta tgttagaagg tgatttcact ggagacccta gcaagatagg cctaagatat
    17461 attaaaatgc ctaactgggt atcagagcta tatacgcgta atacattaac tgctactaag
    17521 ggtgtatggc aaaaactaac tggcaccgtt aaaattactg ctgcgagtga taacgctgaa
    17581 agctggctta taatgctaca aaataaagac gctaataaca gcctatccgg caaactacgt
    17641 ttgagacacg ctaaacttga agaaggctct acagctacac catatcagcc taacttattg
    17701 gcagaacctt ataatatgtg tcgcgaatat cctaacgaaa atattgccga tcctaaagtt
    17761 aagttcccaa tcaaatctgg cgaccaccaa atatatcaag gttacatgga agaagatctt
    17821 atgataggtc aaacgtatac tatcacactt aaaggaacaa aacccgcaag tcaaaccttt
    17881 gtagcgtata atcattggac tgttcgttta ggagaactaa agccagttga tgggttgaca
    17941 gacgtatggt ctctaacatt cacaccaacg aatgttgcgg cgatgcctaa actttttcgt
    18001 gtttatcaat atccacaatc aacagtaggc gcatgccaaa ttgactggct caagattgaa
    18061 aaaggcaaca cacgaacccc gaatattagt gagtataaat atcgtggtac tggtatgcgt
    18121 gattcaaata acccaaaaga ctatgtttgg gatctagcac cagaatatgt cgaagataat
    18181 ttggccacag atattaaaat ttctgaaatt actggtaaag caaacaatta taccgatggg
    18241 aaagtatcgg agattaattc gcagttgact gcttcaatta atgaagtaga caccacagct
    18301 aaggatgctc aaacaaaagc gaatgctaat gcgactgcta tagatgaacg cattaatgat
    18361 acagctacta ccgcatttta tggcgcacaa ggtgaagtct ctaacaaaac gaaagtagcg
    18421 gagtatggag taggaacagg gtatactact acagctgtag gagaaacaca tttcgaacgt
    18481 caatctaacg gatacgttaa atgcttaaaa gctggtaagt attcaattag tgcgcaaata
    18541 cgtgtgcaat tgggcgggaa atatgcttca tggttatata ctgatttata taaagatgga
    18601 acttctgttg ataatttagt atcttatggg gtcgacgcct tgcaaaatag atttgcagct
    18661 agcggaaaca ttgtaactga tttagaagta aacgatgtac taaatatgcg tactgaaatc
    18721 ggcgttgcag atggaatttt acaatttaca tcttgccgta ctttagtctt gactagacta
    18781 tctgattaat acaatattta taattttgct atcaacacgc tcaaaggagg gtgtttttta
    18841 ttttgcaatg aaaggaggct agttggttga aagacgaagc aatacaagac gtggtagaac
    18901 gcttagtgcg tattgaaacg aaactggata attacgaatt attacgcgaa aaagcggaaa
    18961 gtgcaaaaga tagagcggat caggcatatt ctattgcgct taataatgcg gaagatatca
    19021 aagaaatgaa agccaataat aaatggtcgt ggggttacat gatcggttta ggcgttacga
    19081 tcattggcta tttcttgact aaattgtaaa ggaggtgaga agaaatgatt ttacccgata
    19141 agtattatca agtcattaaa tgggcagttt taacagtatt accagctgct tctgtgttag
    19201 tagccacgtt agggaaagcg tatggatgga atggaacaga tatgacagta ctcactatca
    19261 atgcagtagc aacattttta ggtgttatca ctggtgtgtc ggcttataat ttgaaaaaat
    19321 aggaggaaac aaatgaaaaa gaaaattact attactgcga tgagcctatt aacggctctt
    19381 tttttattgc caattaatgg gtttgcctat actatcaaca atgaatttaa tttgggcgca
    19441 aatgaaggta gctcacaagt agcaaataat cagtacattt tactgcatga aacggctaat
    19501 gaaacagcaa caggacgcaa tgaagcgcag tatatgcaac gttcatggac tagtgcttac
    19561 actgcttaca ttgtgggtga tggcggaatt gtttaccaag ttggacaacc tggttatgta
    19621 cagtacggtg ctggttcgta tgctaatgcc aacagtcctg tgcagattga gttacaacac
    19681 acacataata aagcaacttt tgaaaagaac tataaagcat acgttgaatt ggcgagagat
    19741 tccgctaaga aatacggtat tccgcttaca ttggacacgc cttataacca accaggaatc
    19801 aaatcacatt tatgggtaac acaaaacatc tggggcgatc atacagatcc ttatggttat
    19861 ctttctgaaa tgggcgtaag taaagaaaaa ttagcatatg atttagctca tggatttacc
    19921 gatgaaaatc caacaacttc tgaaaacaag cctgtcattg atccaacacg agctggtgca
    19981 gctaatccta cactgacaga tggaacaaac tacgctcata ttgatcagtt tggggaaatt
    20041 gaaaatgcaa acttgcatgt agctggatgg catattgcta actataaata cgagtatatt
    20101 ttcattatgg attacaatac tgggaaagaa ttagctagag taaaagctga tggaatttat
    20161 agaccggatg taaatcaagc gtatgggaca tctggaaatg ttggctatca tgtatctttt
    20221 aacatgcgta acttccctaa taagaaagtc tatgtcatga tgcgtgcaac gaatgatcca
    20281 gaaggaaaca ctaaaggcgg tgcgcaagat ttccatgaca aacgttggta tttaaatatt
    20341 ccacaacgat aaaaaatccc cccctctttg aactgaaccc caaaagttga actatttaat
    20401 ggactgtttc cgatattcta ctggagatag tccatttaac tttagtttta tccttttgtt
    20461 attataccac ctaatatact catttaattt actttgaaat atctcaattg atcggaattt
    20521 ttctcgataa aaaaactctg atttaagcac accaaaaaaa ttttctatta cagaattatc
    20581 taagcaattt ccttttctag acatgctttg aataatgtta ttctctttta attttttttg
    20641 atattgtggc atctgatact gccatccttg atctgaatgt agaatcagtg aaccctcagt
    20701 tccctttttt ttaattgctt gttgaagcat ttcttcaatc agtttatatg ttggactagt
    20761 tgatatacta taactaatta tttctccgtt aaataaatca agtataggag atagatagat
    20821 ttttcttcct tttatcttga attcagtgac gtctgtcacc cattttttat tgggtgtatc
    20881 tactgaaaaa tttcgtttta acacattctt ggcaattttt cctactgttc ctttataaga
    20941 tttatatcgc tttattcgga tttgacaggt aagtcccatt tgggacatta attttctaac
    21001 tgttttatga ttgattgtat atcctttcat ctttaacgct aaagtgactc tacgataacc
    21061 ataagagttt cttgattctt ttacaatcgc tgtaatttct tgctttatct tgctatattt
    21121 atctggctta tctaattttt ttacccagta ataataagtt gacttcgcta attgtgcaat
    21181 tgaaagtaat agattcaatt taaattcttt tttgagctga aggattgttt taaccttgat
    21241 ttcttcttgc tcaaatcttg ttcttgaatc aaggtttcta acttttttaa atatgcgttc
    21301 tctgctctta aacgaataac ttcttcttca agagactcat ctttaagttt tttaggaatg
    21361 tttagcttgg aattcatact aatttttctg cccctttttt ggctctcaag tgaagaagca
    21421 ccgccttctt catattgctc tatccattta cttagagttc tatttgaacc gatattaaat
    21481 tttttagcag tttcttggat agaaagacca tttgtttcca tatattctat aacatcaagt
    21541 ttaaattttg tagtgtagct tttgccacct ccaaccaagc cttcccaacc atgatagtta
    21601 taaatcctta cccaatgtct aaccagtgta cgatttattt gatatttatg tgcaagatat
    21661 ttgtagccgc cttcgttatt taaatagtct gaaactactt ttttcttaaa aacaaatgta
    21721 tatttccgca aaaaaagcac cccttttaat tagatttcta gtctaacttt tggggtgcac
    21781 atcacaagag ggctatttaa ataccacatt gtttcctctt taaaataagt gtctcgagtt
    21841 tcattgggaa cagagattta caagaaaaac taacttaata aaattcgata atcatgtgaa
    21901 tttataatat
//
LOCUS       DAIGOK010000039        21733 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.039, whole genome shotgun sequence.
ACCESSION   DAIGOK010000039 DAIGOK010000000
VERSION     DAIGOK010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.039"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(153..551)
                     /locus_tag="NOA01_001169"
     CDS             complement(153..551)
                     /locus_tag="NOA01_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCI0632376.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(548..1321)
                     /locus_tag="NOA01_001170"
     CDS             complement(548..1321)
                     /locus_tag="NOA01_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HCI0632377.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1318..2001)
                     /locus_tag="NOA01_001171"
     CDS             complement(1318..2001)
                     /locus_tag="NOA01_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HCI0632378.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2168..3055)
                     /locus_tag="NOA01_001172"
     CDS             complement(2168..3055)
                     /locus_tag="NOA01_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCI0632379.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3052..3921)
                     /locus_tag="NOA01_001173"
     CDS             complement(3052..3921)
                     /locus_tag="NOA01_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCI0632380.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4058..5344)
                     /locus_tag="NOA01_001174"
     CDS             complement(4058..5344)
                     /locus_tag="NOA01_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCI0632381.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5617..6195
                     /locus_tag="NOA01_001175"
     CDS             5617..6195
                     /locus_tag="NOA01_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCI0632382.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6195..7124
                     /locus_tag="NOA01_001176"
     CDS             6195..7124
                     /locus_tag="NOA01_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCI0632383.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7121..8857
                     /locus_tag="NOA01_001177"
     CDS             7121..8857
                     /locus_tag="NOA01_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCI0632384.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="NOA01_001178"
     CDS             complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="NOA01_001178"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HCI0632385.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9753..10262)
                     /locus_tag="NOA01_001179"
     CDS             complement(9753..10262)
                     /locus_tag="NOA01_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HCI0632386.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="NOA01_001180"
     CDS             complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="NOA01_001180"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCI0632387.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11563..12654)
                     /locus_tag="NOA01_001181"
     CDS             complement(11563..12654)
                     /locus_tag="NOA01_001181"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HCI0632388.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="NOA01_001182"
     CDS             complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="NOA01_001182"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCI0632389.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="NOA01_001183"
     CDS             complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="NOA01_001183"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCI0632390.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="NOA01_001184"
     CDS             complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="NOA01_001184"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HCI0632391.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16617..17591)
                     /locus_tag="NOA01_001185"
     CDS             complement(16617..17591)
                     /locus_tag="NOA01_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HCI0632392.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17572..19455)
                     /locus_tag="NOA01_001186"
     CDS             complement(17572..19455)
                     /locus_tag="NOA01_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HCI0632393.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19473..21698)
                     /locus_tag="NOA01_001187"
     CDS             complement(19473..21698)
                     /locus_tag="NOA01_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCI0632394.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
BASE COUNT         6199 a         4792 c         3858 g         6884 t
ORIGIN      
        1 agcatatttt gaaaagaaag tctgatgctt agagtagaat ttcataaaaa tttactattt
       61 cataagtatt cacgagaaaa atacaaacaa aaaagcggaa ataacgctca tccgccttct
      121 tgtttgtgtt tatttcctct tcaaacgctt gtctatgatt cagacttttc tctatcgtta
      181 ttttcatcta aaagttcttg cattccgata atttctcgat attgatgaat cttagtgata
      241 ccaatgactt tgtctacatc aatactaaaa caaataccat tacctggaat atcgatgttg
      301 atgccttgac caatcgcatc cattacttta ttcgtcaaac tatcgggtac taatgtcaac
      361 acaatatctt tttctggcga aatgtccaaa cctaaaaatt tccctgaatc atgtacacca
      421 gaacctcttc catgtaagat cgttccgcct gctgcacctg cttcttttga aaagtcgata
      481 acatcttcgc ccattcctcc gtcaacgatc gtcacaatca tgtgaagatt caatgctaca
      541 agatctttca tttgctttcc cccaattttt gcaatctttc tttttcgatt ctgtcttttt
      601 catcttttcg tttataaata tatcctagta ctaaagtgga taatattggt gtcaatgcaa
      661 tcattgcaac cacaccaaac ccgtcaatga tcggatctct tccttcaatt tgtgcagcga
      721 ttgccacgct cattgataaa ataaatgtcg aacacatcgg gcccgttgct actccaccgt
      781 tatcaaaagc cattgctaaa aagatcttgt ccacttttcg tcctaaaata aatgctagcc
      841 cgtatcctgg gatcaagaaa taataaagag agaatccgac gagcaaacgc cacatagaca
      901 atcctactgc tgcgccgatc ccaattgaaa ttaccatcag catgattttc gatcggattc
      961 tcccctcgct caattcttca acctgtttga tcattacatg aatcgccggc tcagcaaacc
     1021 ctactaagaa ccccattaaa aagccaaggg gaaccagtat ataattgtaa ggcaatcccg
     1081 caatagcact gcctaaatgc tgtcctaccg gtacataagc catattgact ccgtgcagga
     1141 aaaggatcaa cccaatagtc gttatcaaga aacctttcat aatgtcaaag aaacgctttt
     1201 tgcgcatacg aaaactaatg atattcatta aaacaaatag gattacgata ggtgccactg
     1261 ccaaaatgac ttccatggta acgatttcga ttccatctat cacttgctgc cagaaactca
     1321 ttggaagatc acccccaaaa tcatcacggc aagaatcggc cctaacgaag caataccgac
     1381 cattccaaaa ctgtcatttt catttttctt ttgtttgatc atactagtca tccctccggc
     1441 aagggccaag ataaatggaa cagttactgg accagtagtc actccccccg aatcaaaagc
     1501 aatcggcata aattcggaac tggtgaaaaa tgaggcgatc aaaacgccta tgtagcctat
     1561 agccatcaat tgataatagg atagcttgaa tacgacccga agcagcgcaa aggctagaaa
     1621 tactccagtg ccgacgctga cgatcccaat cagcaacact ctgccaatct ctccgtcaga
     1681 tatatcatat acttgttgtc ccaaaacttg cacggaaggt tcagcaatcg tgatcacgat
     1741 cccgatagca aatcccaaac tgaccaaaac ggtcaaactt ttctttttga tcatatattt
     1801 ccctactaga tctccgactt ccatcataga ataatctgct ccaaacaaaa agagcgtcat
     1861 tccgatcatc atgattgccg caccaaccag aaatgaagcg aggtcttctc cttctaacgg
     1921 tgcaaaaaca aacgttaaaa tgacaatcag gacagtcatc ggcaatatag cagcaatcac
     1981 ttctttaaaa ttttttagca tgtatcctcc tcctttactc tttaagttta ccttaaaatg
     2041 aaagagtttt cgagcacaaa cttaaaacgt tatcatttaa tttttctcaa cataaaataa
     2101 cacacgtccc aaaagtatct ttttacttgg aaaaatgact ttgaaggaac gcgcttattg
     2161 tgttttttta ttttacagag ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa
     2221 atacgatggc agttggtaaa gtagcgatga ctaatccagc catgatcatt ccatagtctg
     2281 gtgtatagga agcacccata gcagataaaa caagcggaac cgtccgttta tcaggagatt
     2341 gcaaagctac taagggccat aaataattgt tccagctact catgaatgtg atgattgcag
     2401 ccgcggcata ggtattttta gccgtcggca tatacactct aaaaaaaata cttagttctt
     2461 ttagaccgtc taatcgagcg gcttctacta gatcttttgg aaaagattta acattttgtc
     2521 gaaagaagaa gatcaaaaaa gcagtgctaa cagatggcag gatgacaacc aagaaactgt
     2581 tgattcccaa aaccgttccg ttcaattgtg aaaacattcg atataaaggg atcatcaatg
     2641 ccgcaaaagg aaccatcatc gatagcagta gaatattgaa aattaggtct tttcgtttac
     2701 tttgataaat ctcaaatcca tatcctgcca ttgacgaaat cagcaatgca aacacagtgg
     2761 tcaccaaagc gatcatcgct gaattccaaa gggaacgagt aaaattcagc tcattggaaa
     2821 acaaattttg aaaattgatg atcaagttac tgccaatctt tatttttccc gatgtgatat
     2881 ccacgggtgt attcgtcatt cccaaaacca tccagacaaa agggaaaatg gaaataaatg
     2941 ataggatgct taatgctgta tactttaata ctgacccaat tgtcgaacga agttctaatg
     3001 cttgtttttt gacgttgggg cgttcgattg tttctttttc taccatattt ttcatcgact
     3061 ttctcctcct actttcgttt ggattatcga caatatcaca ataaaggaaa caataacaaa
     3121 ggaaactgct gccgcatatc caaaattagg cgtgaattta taactcaaat tatagatgta
     3181 ttgagatagg gtggtcgttg catttgctgg tcccccgcca gtaatatttt gtacttcatc
     3241 aaaaagctgc aatgtcccga tcgtcgaagt aatggaagta aacaaaatga ttggttttaa
     3301 atttgggatc gtcactttga taaactgctg ccatttagaa gcaccgtcaa tagatgctgc
     3361 ttcataaatc tccgaatcaa tattttgcat gcctgaaagg aaaaaaatca tattgtatcc
     3421 agtccaacgc caagtgatcg aaagaatgat cagaactctc gcccaaattg gatgagtcaa
     3481 ccagccaatc ggtgtgtcaa tcataccaat gcccaacaac aagttgttta caaggccaga
     3541 ttgagaaaat agacttttca tgatcagaga ataactgacc atcgaagtga tacatggaag
     3601 aaagatagcg gttcgaaaca accctttata tttcaacttt ttatcattta aaaggttgga
     3661 aataaaaagt gctagaaaca acatgatcgg tacttgtaca agtaaataca ggaatgtatt
     3721 aaaaaaagct tttttaaaag ttgcatctgt caacatccgc tgataattac ctaacccgtt
     3781 aaatgtcata ttgacagggg gaccactttg aaaagacgtg atcaaagcca aaaccatcgg
     3841 aataaaaacc atgattgata tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt
     3901 gaataaatct ttctgcttca ttcttttccc tccaattcta tcattgatta tctacatgaa
     3961 aagacaagaa agacaatatc tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct
     4021 gatgaaaaag cgaagaaatt cactttttta ccaccgatca attagccaat tgtgcttcta
     4081 cttgtttttg tgtatctgct agtaccttgt ctgtctcttc tccattgatg attcgatgaa
     4141 gagcttcagc tacgactgct tcaattgcat aggtctcatg tccgtaattc acttctggta
     4201 tttcagatgt ccatgttgaa aaatcgtcaa agatcttttg accactgtaa aattcagaag
     4261 cttcttgata agcttcttgt tcattcgcgc taagcatcgt agatactagt ccgatttctt
     4321 tcgcaagagt gcccatcagt tcttcattcg aagcaaacgt tgcttctagg aaatctttcg
     4381 ctgaatcttc tccagctact cctttgatca cataccagcc agcgccgcca aggttagaag
     4441 cttgcgcttt ttgcagattt tctggaagtg caggtattgg tgcaatcttc cattttccag
     4501 attggttttc tgctccttgg atcgttgaag aataccaagc gccagttggg ctagaagcta
     4561 ctgctcctga ttgtatcgca gttactccag cattccagtc agaggtttgt tcgactagat
     4621 cttctttcaa aagagtggca aacaattcaa gaccatactt taaagatgca ttgtcttgga
     4681 tcgtgactgt ctccccatct tcagatgtat accattctcc tgcctgttgc ataatcatcc
     4741 ttactcgacc aaggtcagaa ggattgactt cagcgatttt ctttcccgtc ttttctttga
     4801 catctcgcgc tacttgaata tactcgtccc acttcagatt ctccatatcc tcttctgtat
     4861 aacctgcttc tgccatcaga tccgtacgat agaagtttgc tgttacgccc gaatcgaaag
     4921 gaacaccgta tattttatct cctactttgt ttaccgcaaa tttatacggt gcaaaatgat
     4981 cttcctttac aatagcagac aaattagcga atgcatctgg ataagaagtc aaatagcctt
     5041 gtatcctata atcttcaatc aagacgatat tgggcaatcc ttctgtattc ccgctcgcta
     5101 aagcggtgtt cagtttctga acgatatcat cctgagacat cgtaacaact tctatctctg
     5161 tttcttcgtt ctcataaact ttttttgctt cattcactgc tttgatattg aatgtctcat
     5221 cccacgccca gacagtgact ttttcttctt tgtctgctgt actactagca cttccgcatc
     5281 ctgttaagaa acctgatgct gccgctaaac aaacgactcc agcaattaac tttctgatgt
     5341 tcatttttat ccgctccttt tccatccatt agttgtttac gtttgctaca ttcatcatac
     5401 caaaatttga aaacggtttc tttattattc agatacttca aaaatttctc ctaaaataat
     5461 aaaaaaatac aaatcaacta tgacaaaagg aataatctta gcatctagac caaagatcga
     5521 aatggtgagg tttctttaaa ataaaaatat ccttgcattt tctcctagaa ataagtattt
     5581 ttaaattaca agactattct taaggcgggt aaaaaaatgc caatacgcgt aattcttatc
     5641 gatgatgaac agacgatttt agatggaatg aaacagttga tcaattggtc tgagcacggg
     5701 tttcaattaa tagggacttt ttccagtccg ctgaaagcgc ttgatttttc tttggtacat
     5761 gcaatcgata tcgtggtcac agatgtaatg atgcctgaat taaatggaat cgaattatcg
     5821 cggctattaa aacagcaaca acctgaaaca cagatcctta tcctctctag ctatgatgaa
     5881 tttgatatgg ttaaagattc ttttaaagaa ggcgtcgctg attatctttt aaagcctcga
     5941 ttgagtcctg atttttttct aaattcctta actgccgttt cacaaaaaat caaaaaaaag
     6001 cccaacaaaa gcccaatcac caatcgttat gagcagatct gcgagaatct ttcacagcag
     6061 attgctggac aaactcgtcc attgcttgac cctcttcttt ttgaacacac cttgtttttc
     6121 tgttatatac cgaaaaacgg ccattcagtt caaaacaagg ctctaaacaa aaacagtcga
     6181 tcacccattc attgatggat agcgacgatt cgtttactat ttatcctgtt tctacctttt
     6241 cggaagaatc cggctatgta atcaatactg attccaaaga tacacttagg aagtttgttc
     6301 aaaagtttcg tcaagaagtt tttccaggtg atttttttgt tgtttctgat tcttttgcaa
     6361 tccaagatat caatgaaata ttccaagaga tcagaaattg caccaaagga caacgtttct
     6421 atcataaggg aaagaacctt gcttatcagc atgaacttct tccgttatta accaaccacg
     6481 agcagcaagc aagtagttat ttaacaaaaa tcgttcataa agactacgct ttagctgtca
     6541 aagaacttga gaatttcttc acgacgatat tggaacagcc tattgatcca gcttatttga
     6601 aacatgaggc aatcaatcgt ttctatgcat tactcaacgc tttggctgac gagcatgttg
     6661 ggaatgaaga actttctttg ctcaagatcg cccttccttc tttattagcc gatgctgagt
     6721 atttcgaaga tttttctatt cttgtcaaag aaacacttga gaaactcacc cacttttctg
     6781 caaatgcaaa aaaagacaat ttcgatatct tgactttgat ctacgagttc gtccaggaaa
     6841 attatgagaa agaaatcaat ttacaacttt tatccgaaaa atatcatttt tcatatagct
     6901 atctctcagc catctttact gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag
     6961 tccgtatcag taaagccaag ctctttttga cggaaacacg gcttactttg acggaaatct
     7021 gtgagaaagt cggctatacc gaactgggtt atttctccag agtttttaaa gaagagacag
     7081 gaatgacacc ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga
     7141 gcatgaactc tttgccaaac ttctgttcat tttgttcatc ggtctttttc tgcaagccat
     7201 cgtcatttct ttgtttattt atcatcgatc aagggatgcc tatatccagc tgttcaatca
     7261 atcgaatgat gtggtactaa aaaagattca atctgaattt gaaagtttga atgatacaat
     7321 cgagaataca ttagccgctt ttgattctaa tccagctgtc aaaagctatt tttccaatga
     7381 tccagcccaa catatggaac agcttcagca actaagaaca atccaaaaaa tgaatgactc
     7441 tctttcaaaa atccatccaa tgatcgatta cgatgtattg atctttggag aaaatggaag
     7501 aacttttgtc ggaaacgaca tgctcactgc cgtatctgcc gattcttttt ttcaatctgc
     7561 gattgctcag agagtgaatg aacgtgctgc tgatacacaa atgctttttg cacaccacgg
     7621 attgactctt cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac
     7681 attgaatcac gtttacggat atgctgtttt atcgatctca tccaagcaac tagcgaatct
     7741 ttttcaatct gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg aagaacagac
     7801 gatcatcgca tcaaatgaag aaaatacaat tgggagccga tcaatgattt tcaaaaaact
     7861 aacagtaggt gaaacaacga tttcaaatgg acagcagttg acccgtcttc ctctgtaccg
     7921 ccaggattgt acattactca gtcaagtcga tcttcattct ttatctaacc aaatgggcgt
     7981 gatttttccg attattttgt ataatatcgt gacacttgct tttgctggct cattggtatt
     8041 tatgtatttg gatcgacaca caaaatcgat ctaccgcttg attgattcgc taaaacggct
     8101 agaggaagat ggcttaaaag ctactgttcc tgtacaagga agccatgaag tccaattgct
     8161 agggaatacg atcaatcaat tgctaagtaa tgctcagcat cattatgaac agacgatcca
     8221 agacgaaaag aagaaacgtt cgcttgaaat ccaaactatg caagcacaga tacagcccca
     8281 ttttatttat aatacgctga ctagcttgaa atttttgatt tggcagaaaa aaacagatga
     8341 agcagttcaa ggaatcgaca gtttcattac tttactaaga agtacgattg ggaaaaaaga
     8401 agaagtgatt ccagtcaagg aagaaatcaa aagcgtacaa agttacatcg atcttttgag
     8461 tatgcgatat ggcgaaagaa tctcggctaa aataatgatt cctgatgaac tgcagtcatt
     8521 atctatgccg aatatgatcc ttcaaccaat tatcgaaaac gcctatcttc atgcttttca
     8581 caataaaacg caaggattca tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt
     8641 cgaagtgatt gataacggcg atggcttcga tactgaaaat accaaacaaa aaaatgatca
     8701 tttttcagga atcggcatca gccatgtaga tgagcggata cgcctaatgt atggcgatgc
     8761 atatggctta acgatccaat cagtgattgg gacaggaacg actgtaacga tttgtctacc
     8821 gcttcttcct aaaaatcaat cagaatatca aaaataaaga atagccaagc gctgattttt
     8881 ttattttaag ttaaataaat caatcagtat tcttgacttt catctacgcg tctcttctac
     8941 tgtcagcact ggataacagc ccaatatttg actttctcct ccgagcatct tgatttcttg
     9001 gagcgcattc tctactaatg tcattggacg gtccaacaac aaatcgatca cgaaaaaata
     9061 ttctcccaag ctagttttca atggtcggga ttcaatctta cttaagttga tttcccgcca
     9121 gccaaaagct gccagcattt tatgaagcat ccctggccga ttagcaggta gtgtcaagat
     9181 gacactcatt tttactggag ctgactgctg gctggtcatc tttcggtcac cgacgatcca
     9241 aaaacgtgtc tggttcaact cattatcttg gatattctca gctaagatct ccaatccaac
     9301 atgctgtgct gtttctaatg aagcaatcgc tgctgcgtct tctttcggat gttctgctac
     9361 atacattgct gcagcagttg ttgattctgt tgctactaaa ggaacattag gataatgcgt
     9421 ttctaaaaac tgttgtgatt gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt
     9481 tgttgctgga ttgcctaaaa gctgctgctt gataggaaga acgatttcac ttttaacttc
     9541 gacttctgga tgtttggaaa gaagatcgat tgtgtgatga acagctcctt ccaatgaatt
     9601 ctctacagga acgacagcca gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg
     9661 gatggaagca tacgcagtca attgttctgt aggaaatagc tgacaagccg cctgataagt
     9721 gaatgaactt tctggcccta gatagctgac gttcatatgc ttttgatcct ttctataatg
     9781 gtatcgatga tcttttctgg tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct
     9841 tcataccaag aaagtcgttg tgccaataat gcttttattt cttctcttgt ttttccaaga
     9901 gctaaaggtc gaatagacgt ttcatcttgt tggatacgat caactagaca gtccgcttct
     9961 gcctctaagt aaagaacaat cggctgtgtt tttaaaaaac gccgattttc tgcactttgg
    10021 acaatccctc caccagtcgc gataattttg tcttcatttg acagctttct taaaatatct
    10081 gactccactt ttcgaaagct tttttcaccg taatagtgaa aatattcggc aatcgacaat
    10141 tggatatgtt cctcaatctt tgtatctaaa tctacatact ctttttgtaa acattgagct
    10201 aaccctttac cgattgttgt ttttccagcg cccatgaaac caatcagcaa aatgctagcc
    10261 atgagcttct cctcctgaca caagttctgc taaatcgtca aaaaatgccg gataagagat
    10321 tgaaacagct tcagctcctt ctagttcgca aggtccatca gctatcaaag cagctacttg
    10381 cagcatcata ccgattcgat gatcgcctct gctggacact tttgcgccat gcagaggtgt
    10441 cggcccatgg atgatcagac cgtctgctgt cgcttttatc tttgctccca ttttctgtaa
    10501 ttcttctgcc accgcatcaa ttctgttggt ttcttttact tttaattctt ctgcgtcttt
    10561 gatgatcgtg attccttctg cttgagtagc cactaaagct agaatgggca attcatcaat
    10621 cagacgcgga atgatttctc caccaatcgt tgctttcttc aaatgactcg tcttgacact
    10681 taaatcagcc gattggttct gttcattgtg attcgtctgg gtaatcgctg cgcccatctc
    10741 ctccaacaca tctaagatac cagttcttgt tggattgacc ccgacatttt ttaacaacag
    10801 ttggctttcc ggaacaagaa gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc
    10861 tggaacagtt atctgttgac cagttaattt ttgcggtccg gtcactatga tcgttttatc
    10921 ttccactgtg atccttccgc caaattgacg aatcatttct tccgtatggt tacgggaacg
    10981 ttctttttca acgattacgg atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga
    11041 tttgacttgc gcactggcta caggcatatt gtatcggata ggtgataaag agcgggttcc
    11101 tgtcacagtg attggtagat actggtcatt ttcttctcct tgcaagtcag cgcccatctc
    11161 gcgtaaaggt tccatcactc gccccattgg acgtttagac aacgaagcat ccccgaacag
    11221 agtagtcgta aaaggctgtc cagccaaaat acccatcaat aaacgtatgg tcgtaccgga
    11281 gtttcccatg tcaattgatt cggttggttg gacaaatgag tggctaccgc gaccatgaat
    11341 cttgatcacc tcttcctctt cttcgatctc gacacctaat tgttggaagg catgtaaagt
    11401 actcagacaa tcttctgcgc gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc
    11461 tccaaacatg atgctgcgat gggaaattga tttatctgct ggtatcctaa cagtccctct
    11521 taatccatgt atttgttgta ataattgcat ttatccagcc tcctatttga tccagcaatg
    11581 ataactggta ttagacataa tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa
    11641 actgatttgc agtattccta aaatatcttc cctggtctct tggatcttca aattaatcaa
    11701 agaaactccc gcttttccga tcagtccagt tacttctgca ataacgccag gttcatctgg
    11761 cacatcgata aataaatcaa aaaaggcagg aatcgcgcct tgcttatgga caggcaactg
    11821 gtctcgactt tcttttgccc gattgaaaaa agaaaagatt tccgattggt tctcagtcat
    11881 gatccattct gctatttgtg tcatttcttg ttgccaatct gccaatcgtt ccaataatat
    11941 ctgcttattg ctcaacaaaa tatctgtcca catgacagga tctgaggaag cgatcctagt
    12001 gatatctcga aagcctcctg ctgccagttg tttggctcgc ggatactctt gattgaattg
    12061 ttctgcctga ttgactaagc cagctgccag aatatgtgga aaatgactta gcattcctgt
    12121 tatacggtca tgttctcttg ctgtaagagt tacatatttt gctttggttc cgcaaaatag
    12181 ttcttgcagt ttttcaacat cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc
    12241 attttcaaat agtttagcat ctgctgcctg tatacctgat ttatgagaac cagccattgg
    12301 atgtccaccg acaaaccgaa gatttttctg ctcagcggat gtcacgactt gctgcttcgt
    12361 actgctgaca tccgtaatga tgacgtgatc agacaagttg attctttcaa ggacatcaag
    12421 ataagctaag gttgtacgga cgggaacggc taataagata acgtctgctt gttctgcacc
    12481 tgtttcaaaa tcacttgcaa cgtaatcaac gatctgctgt tctactgctt gctcacgggt
    12541 ttttgactga ctatcccaac caactactat cagttctgga ttggtctgac gaatacaaag
    12601 tgccatagaa gcaccaatca gtccaagccc cacgataaaa acatgttgtg ccatcttatt
    12661 tgcccccttt ttagtaatgt ttcaagtagt cacggtactc cttcactgca gctttcaact
    12721 caacaagcga atcacttggg aattttgaca atagctctgt cgctaccaca gtggctacaa
    12781 cattttccgc aacgacgcta gctgcaggaa ctgctgtact atccgaacgt tctacacttg
    12841 ctttgtaagg ttcttttgta tcgatatcaa cactttgaag tggcttgtac aatgtaggaa
    12901 tgggtttcat gaccccgcga atgacaagtg gttcgccatt ggtcattccg ccttcgaagc
    12961 cgcctagatt attggtacgt cggaaatagc ctctttcttc ttcccatagg atctcatcca
    13021 tcacttctga gcctggtaat gaaccagaag tgaatccgac cccaaattct acgcctttaa
    13081 aggcattgat actaaccaca gcttgtgcga ttttggcatc tagcttcgtg tcccattgta
    13141 cgtagcttcc taatccagct ggaacgcctc caacgagcac ttctacaatt cctccgatcg
    13201 tatctccatt tttctttgtc tgatcgatca aatcttttat tctttgctca actgctggat
    13261 caacaactcg tacttctgac gcttcagaaa tttctttgat ttcttgaatc gagatatttc
    13321 caggtatttc tgcttggatt ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa
    13381 gttcttccag aattttcttg gcaattgcgc cgattgctac acgcatggtc gtctctcttg
    13441 cagaggaccg ttcaagcaca ttccgaagat ctcgatgatg gtatttcata ccgcctacca
    13501 aatccgcgtg tcctggacgc ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca
    13561 cttctgctac agacatgaca gttgtccaat ttttccaatc tttattttct accactaaag
    13621 tgacaggtga accaattgtt ttgccgtgac ggatgccaga cgtgatccga acacgatctt
    13681 tttcgatctt catccttcct cctctgccgt aaccagcttg tctgcgagcg agttcgtgat
    13741 tgatttcttc ttctgacaga ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt
    13801 ctggtccatg ggattctccc gcagtcatat aacgcatctt acagtcctcc taagtaatct
    13861 ttgaatgtct ctgtgggaac aggaacgatt tttgcttgcc caatcttttc tagcaagatg
    13921 atattcaatt ggcttcctct tgctttctta tcatgggtta tcgcgtggaa taattcatct
    13981 ggatgactta gatcgtacgt aatcggcaga tggaattttt gaagtatcct ttctaaagca
    14041 gctgtcgatc cagttggaga caagccgatt ttttctgcat gagaagtgat ggctaccatt
    14101 ccaatagcta ctccttctcc atgactgact accccatatc ctgctgtatt ttcaattgca
    14161 tggccaattg tatgaccaaa atttaaaata agtcgatttc cttggtcaaa gacatcttct
    14221 tccactactt ttcgtttgat ttccaacgta gcagtaatga ttggaacagc atgttccaga
    14281 agatcttttt cgtctttcag ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct
    14341 gctgatttga tgatttcagc gattccttct cgtaaacgac gtaccggcaa agtttttaga
    14401 acctcaggat caatcaatac accagctggc tggctaaatg ttccgacaag gtttttcgct
    14461 ttgtttgtat tgactgctgt cttcccgcca atactagaat ctacttgtgc aagtagcgta
    14521 gtcggaattt gaacgaaatg gatcccacgc atgaacgtcg aggccacgaa tgctgctaga
    14581 tcacccacca ttcctccacc aagtgcaagt atcccatcgc tgcgggtaaa atcattatct
    14641 gctaaaaaat cgtataactc agctgcttgt tctaaagatt tactggtctc acccgccggt
    14701 acgattgaaa ggactggttc aaaattagct tgtctcaatt ggaacatgat tttttctgca
    14761 tatagtggag caacgtttgt atcagtgatc acagctactt tttgaggtct ccaaatcgtt
    14821 tgaagccatg atccagcctg ttctagtgcg cctttttcaa tatgaatatc gtatttttgg
    14881 ttatcaacat gtacagtaag cattcgtttc acttctctct tttaaaaagc tggaattaat
    14941 cgagtgtctg aatcttttcc attgcttctt tcatgatcgc aacttctttc atcatgcgtg
    15001 agtaagtctt ttcattcaac gattggggac cgtctgacca agcatttgca ggatcaggat
    15061 ggatctctac gatcatgcca tctgctccgc ttgccacacc cgcacgagcc attggtggta
    15121 caagttccca aattcctgta ccatgacttg gatcaacaat cacagggaaa tgagttaatt
    15181 ttttcatcaa tgggacagca cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc
    15241 gaatcccacg ttcgataaaa atgacaggag atttgtcttc gactgcaatg tattctgcag
    15301 cattaagcca ttcattgatc gtaccggaaa ttccacgttt caatccaaca ggtttacctg
    15361 ttttaccgac agcagatagc agtttaaaat tttgcatatt acgtgcgcca atctgtagga
    15421 tatcgctgta ctcagccacc atatcgatat gcccttcatc catcacttca gtaatcactt
    15481 tcattccaaa ttcatcagcc gcttgacgga tgtatttcaa tccttcttct tctaaacctt
    15541 ggaaagcata tggagaagta cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc
    15601 cggctttggc cattcgcgca cattctctga tttggtctaa tccttcgata gaacaaggac
    15661 cagccatcgt cacgaaactg ccgtcaccaa tttttactcc gtctacatcc acgactgtat
    15721 tgccaggatg gaattctcga gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt
    15781 ctactccttc atagcgtgta aatgctcctt cttgtaaact tcgcgtatca ccttttagtc
    15841 caatcactac tcgttctgtt ccttgatcga ttgagacttt gaatccttct ttttcgatat
    15901 gatcgacgac tttttttatt tgttcttccg ttgcttcttt tttcataatt actatcattg
    15961 tttttcccgc tttcttttgt ttacttatct atcttggctg attttctcta ctttttcaag
    16021 gtggttttac attaattcct actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt
    16081 ctttcctact tatgacccag ataatcaaca aaaaaagatg tccctgtagg aaattgcctc
    16141 tacagtgaac atcttcaacg agccttaaaa agcgctgcaa aaatataaaa tagccactat
    16201 agatgaattt ctctacgtgg ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac
    16261 aaacctggtt tgtttgtacc cacgaagtca tgaatacaaa cctatctaaa atagctaaag
    16321 taatacgtat tcagttttgt aagttttcta gatttcataa aaaatcgctc caaactgatg
    16381 taagaattgt acctagtata gacataaaaa aatcgactgt caatcatttt tatgaaactt
    16441 tttttaattt tctgatagtt aactttgttt ttcagaaaaa taacaattgg ttttattgaa
    16501 gaaaatagtc atgaacaaaa gtatctgaga acttttttac tctcttgttc atgacatata
    16561 gaataaagca gctttgtctt gtaaactccg tttttcaaat aataatctaa agattgctac
    16621 tgaataataa agccgatttc atggtcctct tcgctgctga ttcggtaagg tgactcaaca
    16681 tccgctcccc agctattgat acccccgact cctcttactt ttgcaaaaat cgtaagaacc
    16741 gttcgtcttt ctggcggtag ttcttctata tgtgttgcct gttccagttc ttctgctttg
    16801 aatggtaaag cggaaaaagc aaatggcgta tcctgctgtt caaatcgcaa cgaaaaatct
    16861 tcttgatctt tatccgtatt atccaacgtt tgatttcttg ttagttctac ccatttcgtt
    16921 tccatatgca tgccgcattc ttggggaaca aggtatgggg aaacggggag tcctgccact
    16981 ttataaatac cttcgacacc accagccatc cgatccggat atgtttcccc tgataggcct
    17041 tcatactcaa aaaattttgc tttcgttggc ataatgaacc ttaaacccaa tgcgggtaat
    17101 tccggtaatc ctttttgtcc ataataatgc atattgacaa gcattttacc ctctccagcg
    17161 atttggtagg aaattgtcac ttgggttttt ggtactgtgt tgatttccag tgtaaaaatg
    17221 atttcaaccg tttcagcgta ttcattactt gaatattgat tatttactgg agcaatcggc
    17281 aaatcgatct ttttattgtc caccagtact tctatttctt tgacttttgg atacaatccg
    17341 gctcctaacc acataccgga tttaaatcca aatccgctac ctctgtcatt atcggtcgtt
    17401 gctcgccaaa aaactggaag tgtttcccgg tatagccatt cttttccgtt tttattgagc
    17461 gattccagtc cgcctcttgt ataagaaaaa atataatgcg tattccccga tttgacacct
    17521 aatgtacagt cgccgaagac gattgcaact ttttccgtat ccatatgact tttatttact
    17581 gtatttttca taataatatc ttacctcctg catggcgggt ttttcttgtc catctgcaaa
    17641 aacgatccca ttccctgaga attcatagtc acttggttta tcgtcaaagt caccgccata
    17701 gcgcatcact tctctgcctg tgattggatc agtcacttgg atcgcctgat ctttaaaatc
    17761 ccatatatag cctccttggt acatttcata acgatcaagc aacgtcatgt aagaatccat
    17821 tccacctaac gaattcccca tgtcatgcat atattcacat aagatgaacg gtttttccgc
    17881 tttattttca aggtagtcaa tgatctcttc tggtggcgca tacatgcagc tttttacatc
    17941 tgaaatcgaa tcttcgaatg ctggatttgg aaaattccct tcataatgga caagtcgatc
    18001 ggggtctact tttttgaagt aatcgttcat ttgtcgaatg ttttcttccg cataagattc
    18061 atttccaaga gaccaaaaaa gaatacttgg atggtttttt aatgtttcga aattcgtttt
    18121 tgctcgatca agaacagccg cctgccactg aggtactgca cctggtacgt tgtaagaagg
    18181 ctcaaatgcg cccatttttt gccatgagcc atgggattcg aggttcgttt ccgccatcac
    18241 atggattcct gcttggtcgc ataaataata ccaagtgatc tggttggggt aatgagatgt
    18301 ccgtacgccg ttgatctttg cattttttat gatagccaaa tctttttcca tatcttctat
    18361 agtgatggcg cgtccttttt tcggatgcca ctcatgtcga tttaccccgt tgataatcat
    18421 ccgttttcca ttgaagtaga tgattttatc tttgatttcg attttacgaa aaccgaagga
    18481 atagtcaata tactccgtta ctcttttttc ttcgtctatc gtctccaatt caagcttata
    18541 taaattgggc tgaaaatgcc cccacagtgc cacattttca aatccatcga tgcgaatcgt
    18601 catttgctct tttaacggat attgctgttg atagatttcc ttcccatcag gatctagtat
    18661 cgctacattc agctgactgt ttttggctac ttgctcaaaa gacaaatcta cttcaagttt
    18721 gccattttga catttgtcat ctaagcgagg tttgatccaa agatcttcta aatggttttg
    18781 tcctcgagca actaagaaga catctctgaa gatcccaaaa aatcggaaaa agtcctgatc
    18841 ttccaagaat gcagctgtac ttcttttgta aacctccaca gctagaatat tttcttcttc
    18901 caaataaggt gttaaatcaa attcagacgg agtgaacgaa tcttcggcat acccgataaa
    18961 atgaccattc aaccaaataa acattgcttg ttctacacca gcaaaatgaa tcgagatttg
    19021 ttttcccttt agaccttctt ctaatcgaaa agtcgtcaaa tacgaaccaa ccggattgtc
    19081 tttagcagct gaaaagatcc cttccgcctc tttctcaggg cttgtcgtga aagggggacg
    19141 gcgaaataca tgtccctccc acggatacat cgtattgatg taatggatct tgtcataatt
    19201 tgctaattca atatgactag gaacagtgat ttcatcaaaa gaacaggcat caaaatcttt
    19261 tttataaaat gtttttggtc gtaccgaagg atttggcgaa taacaaaaac gccatgatcc
    19321 atttaaagac tgtttaaggg aggatgtggt ttctacttct tcccttgttg cataacaaac
    19381 atgatcgcta tgtgcatttg attggttcac tctaaaaact tgtggatcat ctaaccattt
    19441 tatatttgct ttcatacttt tttccttctc tctcataact ttttgaaatg aagcagttct
    19501 gtttggaaat caccttgaaa atcggggaag taaagtccaa ctgtcatcaa ttcggccccc
    19561 gaaaatatcc gcttcgaggt ctgttcctga taaagtgcct catcatctaa ccccatcaga
    19621 tatacttcat ggaacgctgg ttgagctgaa gctagtatcc tccctaaaaa cacaatagct
    19681 tcatcttgtt taggagacac aaacatccat gcggcaatat tttcttcaaa aggactcgcc
    19741 aaacgataat aatggccata ctggatcacc ggacgaatcg tttgatagga tataatttgg
    19801 tcttttactt gctttttttc ttcaggagac agctttgtca aatccagttc ataaccaaaa
    19861 acagcgctca tagctgtttc tcctcttgtt ttcaacgaag tagatctgtc ggtctgatga
    19921 tttggtacag ctgatacatg agctgtgatg gacgaaatcg gataagcaag gcttgttcca
    19981 tattggattt tcatacgctc cactgcatcc gtgttatcac ttgtccatga ctgcggcata
    20041 taataaataa acccggcatc aaaccgtcca cctccaccag aacagccttc ccataagatt
    20101 tccggataat cagttgtcaa atgttcaagt agttcataaa gcccgagcat ataacgatgc
    20161 gctacttctc cttgtctctg tgggtcaagg gtaatcgaat agacatctga aagcgaacgg
    20221 ttcatatccc atttgatgta gtcaagagga atcgtatcta aaatctttct catttgtttc
    20281 tcaatcgtct gccgaacatc taaccttgta aaatccagta catgttgtga tctggaagca
    20341 gaaggcattc tacctggctc ctgcataaga aaatcaggat gtgttcgata cagttcagaa
    20401 tcaaccgaga tcatttcagg ttcaaaccat aaaccaaatt tcaatccttt gctatgagca
    20461 taatctgcta tttcacgtaa tccatttgtc aacttgcctt catactcgaa ccagtctcct
    20521 aatgaagaat tgtccgcatc tctttttcca aaccagccat catctaatac gaacatttcg
    20581 atgcctagct cctttgcttc atcaatgatc gccttgattt tttcactatt gaagtcaaag
    20641 taagtggctt cccagttatt gaccagaatc ggccgttcag caaattgatg acgacctctt
    20701 gcgattcttt cacgtagcaa ctcatggtgg atctgactca ttttgttcaa tccctgactt
    20761 gagtaagaaa ggatcatttc aggtgtttga aaagactggc ctgcattcaa ttcccacgaa
    20821 aaacgatggc tgttgatccc accgacgact ctcaattgat tgatctgatc tttttcaagt
    20881 tcgaaagaat ggttgccgga ataaacaaat tgaagtccga tcgcttctcc agtattttct
    20941 gtcgtatgat gatgaactaa agcaataaag ttattcatat ggtgactgct cgttcctcga
    21001 cggctttcaa atacttttgt tccagataaa acagattgcc gtgaaatctg tctttcattg
    21061 acatgtgctc ctggcaaagc aatgacttca tcaaatcgcc tagtatgggc aaagtctagc
    21121 tggaaactag ccgctttttc tagtttgatt gtttctccgg tttcatttct tatctttaca
    21181 gatcgtgtga tcactgctct atcagtaaat atggtataga acaaatcgaa ataaatcgct
    21241 gtttctctat ccttaagtga aatcaccaac gtttgtgcct ctgacttgtc catcacataa
    21301 gtttgaggca agccttcgat ctctggcttc ccatctgtaa tataatggct gtcatatctc
    21361 agatccagta agtctgatcc attcttgcgc tgaatgatcg atgcgggcgt tcgaaagtcc
    21421 atgctgccat gagaagagta ttcttgcggt agtgtatctt tcgacagaga ccggtcagga
    21481 tttaagggaa catttcctga aaatccacga tctcttcggg gataattttt attgtctttg
    21541 tactgcttga ctggtttgcc aaaataaata tgcgccaata cattgctttc ttctatcttt
    21601 atcagataag aaagaaatgt atttcgtaaa tggaagactc ggctcttttc atcaaataag
    21661 atgaaatttt tatctgtttt tctatgcgta atatccatca tttcactccc aacaaaacgg
    21721 aaaccgttta ctt
//
LOCUS       DAIGOK010000040        21730 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.040, whole genome shotgun sequence.
ACCESSION   DAIGOK010000040 DAIGOK010000000
VERSION     DAIGOK010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.040"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(173..1072)
                     /locus_tag="NOA01_001188"
     CDS             complement(173..1072)
                     /locus_tag="NOA01_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCI0632395.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1082..1438)
                     /locus_tag="NOA01_001189"
     CDS             complement(1082..1438)
                     /locus_tag="NOA01_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCI0632396.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1793..2089
                     /locus_tag="NOA01_001190"
     CDS             1793..2089
                     /locus_tag="NOA01_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HCI0632397.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2733..3686)
                     /locus_tag="NOA01_001191"
     CDS             complement(2733..3686)
                     /locus_tag="NOA01_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HCI0632398.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3709..4464)
                     /locus_tag="NOA01_001192"
     CDS             complement(3709..4464)
                     /locus_tag="NOA01_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCI0632399.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4461..5420)
                     /locus_tag="NOA01_001193"
     CDS             complement(4461..5420)
                     /locus_tag="NOA01_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCI0632400.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5413..6363)
                     /locus_tag="NOA01_001194"
     CDS             complement(5413..6363)
                     /locus_tag="NOA01_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCI0632401.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6513..6965
                     /locus_tag="NOA01_001195"
     CDS             6513..6965
                     /locus_tag="NOA01_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HCI0632402.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(7054..9321)
                     /gene="gshAB"
                     /locus_tag="NOA01_001196"
     CDS             complement(7054..9321)
                     /gene="gshAB"
                     /locus_tag="NOA01_001196"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HCI0632403.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9508..10506)
                     /gene="ftsY"
                     /locus_tag="NOA01_001197"
     CDS             complement(9508..10506)
                     /gene="ftsY"
                     /locus_tag="NOA01_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCI0632404.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10517..11326)
                     /locus_tag="NOA01_001198"
     CDS             complement(10517..11326)
                     /locus_tag="NOA01_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCI0632405.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11335..14916)
                     /gene="smc"
                     /locus_tag="NOA01_001199"
     CDS             complement(11335..14916)
                     /gene="smc"
                     /locus_tag="NOA01_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HCI0632406.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14936..15622)
                     /locus_tag="NOA01_001200"
     CDS             complement(14936..15622)
                     /locus_tag="NOA01_001200"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCI0632407.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(15970..17751)
                     /locus_tag="NOA01_001201"
     CDS             complement(15970..17751)
                     /locus_tag="NOA01_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCI0632408.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(17777..18691)
                     /locus_tag="NOA01_001202"
     CDS             complement(17777..18691)
                     /locus_tag="NOA01_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI0632409.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(18718..19680)
                     /locus_tag="NOA01_001203"
     CDS             complement(18718..19680)
                     /locus_tag="NOA01_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI0632410.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(19680..20624)
                     /locus_tag="NOA01_001204"
     CDS             complement(19680..20624)
                     /locus_tag="NOA01_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0632411.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(20625..21629)
                     /locus_tag="NOA01_001205"
     CDS             complement(20625..21629)
                     /locus_tag="NOA01_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0632412.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
BASE COUNT         6070 a         4640 c         3847 g         7173 t
ORIGIN      
        1 agaatacacg cttgcatcta gtactctgcg agttacaaat catccgatca aataaacatt
       61 cgctgattat tttccgcaaa aaaagcagca tatccacgat aaatggatct actgctctta
      121 cttatcctta tgttttacaa ggtattttct tttacacatt actaatgctg tctcaatacg
      181 gaacgattgt catcgcttgt tcaaattcct cttgcaaatg ttgtgtttga taggcctgaa
      241 aagctgggtg atgggcacgc aagaggacac gtaatttcat aggatcatag ctgtctagca
      301 actgttcttt caagacaaaa tctcgggtca gctgccattc ataataataa ccaaaaggtc
      361 tgcagctgta aatcgtgtga ccgatggatt tatcttcaaa gcgacgatgc gtatggccaa
      421 aaactacttg tttgatattt gaataacggt caagtaattc tccgaatgat tctgatccta
      481 aaaatgcatt taatttgttc caacgctcat aaggagcaga ttgatacatg ataaatgccc
      541 gtttaggaac gaaatgagtg gataaaatca cctcaagttt ttttgcttcc agctggtcta
      601 gcaaattttt tgcagctgca agcatttctg cattgacctc gagatctgtt ttcccacgct
      661 ggatgaaccg atcataccaa tacaaacgct tcattcgtac gattgcctcg taatctttca
      721 attcggaaaa ctgatagtcg taccagccat tcatccctaa aagaacggtc tgctcattta
      781 aagcaatata ccgttcattt aaaaaatgtg ggtctggaaa atggtccatc atctgttcat
      841 tgggcacatc tgccaactcg tgattgccga aatgaaatgt cgtagataat cctttattga
      901 cgaaaaattc gacaacagct aggcaacggt ctactttatt agccgtatct ccggctagat
      961 gaagccgagt gatcttgtgc ttttgcaaaa cttgccaaag ctgcatcagc tctgattcgc
     1021 cgaatttatt gatgtccaca tgtaggtcgc tgatgatcgc caattttccc attttatccc
     1081 tctagtcttt tcgaacaact tgcaagttgc cttttctcaa gccgcttggt ccttttaatt
     1141 gtaaactatt ataagttggt tctaaagcaa gtgtggatgg atcatccatg taaagtattg
     1201 cactgtcttt gattttgatt ggtccaactg gtgtatcagc cactacagaa taaagactta
     1261 gatctgtttg tgtatctttt ttgacaatga tcaatcgaaa tgccgtatcc aattggcaac
     1321 tgacttggct ttctgcaaat ggaccgtcac cgttttcgat atctaataaa agttgatcgt
     1381 ctgcttctaa tttattcttc aatacttctt gtgcttcttt tgtcacttct aatttcatct
     1441 gtatcacctc atgtcataga ataatcactt tattcatcag atgcaactaa tttgtctggt
     1501 tctgactgtt ctcaatttat tggaaagtaa gtcattcgtt tattatgatt tctcttatca
     1561 caaaagagac aactttccct tttcttcgtt cagtttcttg aacggaaaaa gacccaaaaa
     1621 gcaacctgtt atttgtttaa ccttattcct ttcttaaata acaaaaaata ctataaaaaa
     1681 taaaaaacct ttaacaacta ttggaaaaag aattactttt tgttcacgga ttattaacaa
     1741 ctcttcggta gagtaaactt atcaaataaa agacttcttt ggaggaatca ctatgaagga
     1801 atatataaaa aacatctatt ttatcgaaga aacacaaaat atcgagggtt cttatattga
     1861 agtaaaaaca ctctttgtca atgaagataa aaccaaagca ttggatattt ataaaaaatt
     1921 agcttcaaaa aaaacgaata gttttggatt gatattaagc gagtataaaa tcaaagcaga
     1981 agagagttat ttctatcaat tattaaaacg gtggagtaag cttcccgccg atttttatcg
     2041 aaaaatgcag atcatcaact atcaaccgct tgctgagaca catgcctaaa tagaggttgt
     2101 gaaatcagtg ctttgagcta ataccgaagg tcagctgatt gaacattgtt tagaagagat
     2161 tgtgacaact gcgatttgct ttgagtaata agaacgctgg acagaaaata gcttctcata
     2221 ttttctagtc tagtgggctt attgccgaga agcagcagat ggaacatcgt ttagaagaga
     2281 ttgtgacaac tgcgatttgc tttgagtaat aagaacgctg gacagaaaat agcttctcat
     2341 attttctagt ctagtggact tattgccgag aagcagcaga tggaacatcg tttaaagaag
     2401 agatcggtac aaaataaaaa tatctaaatt taagttttat ctaagtttcg ctttgtacct
     2461 ttgattcaaa tcaaaggagg cgatcttgat gctgaaaaaa tggatcacac tattttttgc
     2521 cactccccat tctagaatca acccgtatga tgtagataat gaattcacgt tgaaaaaaga
     2581 tttctcaaat aaataaaaac catccgtaaa atcatcaata cgattttacg gatggttttt
     2641 gataacttct ttgaggatag gctcttgcta tttaagaatg taagaaacat tattgttaca
     2701 tagtatgaga gtttaatcct ctgttattta tgctatttaa atgcttgatt cacatcttcg
     2761 atcatcagtt tcattgattc taaaccgccc ccgcttaaat accagacatc tggttcaagg
     2821 gagatgatct gctgattttt accagcattc gtttgtgcta ccagttcatt ggcagaaata
     2881 tcatctttgc tgtcgtctcc accaatagct tttgttctgt ctactacaaa taagatatcc
     2941 ggattttttt ccagcacata ttcataagaa acactttgac cgtgagtgga tgcttcgatt
     3001 tgatcatctg cttgttcaaa gccaaataaa tcatggatga aaccgaatct agaaccagat
     3061 ccgtaggctg ataattgccc ttcattcaca agtgtgacta gtgctttttt gtcagtagct
     3121 tctgctttct ttttcgtctg atcgattgca ctagataatt cctctaattg agttttagct
     3181 tcttcttgct taccaaagat ctcccctaaa gcagtgacat tttgctggaa agattcccat
     3241 ggatttttcg catctaaaga aaggaaaatg gtcggagcaa tttgacttag atctttttga
     3301 aaatctcttt gtcggccaga aatgatgatt agatctggct gaagctgatt aattttttct
     3361 aagtctggtt cttttattcc acctgctgat tctacttttt tataggatga aagatactca
     3421 ggaaggctgc ttgtagctgc tccaactacc ttatctccaa caccaagagc atccatcgta
     3481 tctaaagaac cattgtcgaa tacgacgact ttttctggat tatttggcac ctcgatttgg
     3541 tctccattac tatctgttat cgtcaatgta gtttcagttt gtgtgctgct tttttttgtg
     3601 tctgcctgac tggtgttgtt gctatttcca catgctgcta aaccaaatga tgctagaatc
     3661 aagataccta ctactaattt tttcatctct tctctcctaa tgttagtttt agaaataaag
     3721 acagaatctg cggccttcaa tctcacaaat ccgtatgtcc atttcgtata attcatccaa
     3781 tacatctttt tttatcattt cttgggtcgt tccttgcgta aataactgtc catctttcat
     3841 tgcaacaatt tcgtctgcat aacttgcagc aaagttgata tcatgaagaa caataacaat
     3901 tgttttcccg atttcttgta ctaaattttt taaaatctgc atgatttgaa ccgcaaaatt
     3961 catatctaga ttgttcaaag gttcatccag taaaatataa tccgtgtctt gcgctagcac
     4021 cattgcaata tacgcacgtt ggagttgacc gcctgaaaga gtgtcaatcg attgatccgc
     4081 tatttcttct aacccaaggt gttccagtga ttcctgaacg atttcctcgt cttttgtcgt
     4141 caattttcct ttcgtataag gaaatcgtcc aaattctact aattctttca cggtgatttg
     4201 gacatttatt tcattcgatt gtcgtaggat cgataatttt ttggctaatt cattggattt
     4261 ccaagccttt acttcgttat ggtctaaata gatttcacct gtatcttttg gtatcaaacg
     4321 actgatcatc gacaacaccg ttgactttcc agcaccattt ggaccgataa aagcagtcaa
     4381 tttcttttca ggaatcgtta actgtacatc tttaaccact gattttccat ggtacagttt
     4441 attgaccttt tgtaaatcaa tcataggata ctcttccttt cgcgaatcaa ttttgtgaca
     4501 aaataaattc ctccgacaaa ttcaacgacg acactcactg ttgtattcca gccgaaaatc
     4561 tgctctactg cgagctgtcc tcctgtcaga aacagcatag ctaccagaaa gcttccgata
     4621 aatagctgac catgacgata agtagaaaag acacgataac tgatatttgc gacaataaac
     4681 cctaaaaaag taactggacc cacaagagcc gttgatgtcc ctgtcaaaag actgacacaa
     4741 cataaaatca aaaattcaaa acgatctgtg tctatcccga ggtttactgc ttgttcattt
     4801 ccgagatgga aaacgtctag cacatgtcga tatcgaaata acaggtacca gcagccgata
     4861 atgatcaaag aagcaggcaa aagatactga ctctggatat tgccaaagct tgcaaataat
     4921 ctgccttgaa gcaaatcata ttcattcgga tccatgatga cttgtaaaaa ggtactgaca
     4981 ctaccaaaaa gcgtacctaa aatcatccct gacatcagta ataaaaaaag attcgctctc
     5041 atttttttca aaagaaagaa aaataacaat agactcagcc cgaccatcag cccaacgttc
     5101 aataagaaaa aaactgtacc tgtctgctga agcatcgtca ctcctccgat aaagaagaaa
     5161 aggaccgttt ggacaaggac atataacgac tcaagtccca atactgaagg agtcaaaaac
     5221 tggttatgcg taattgtctg aaagctgatt gtggcactgc tgacagctag cgagacaagg
     5281 ataaatgcaa aaattttctt actcctcaat gcacatgcaa aatgccagtt tccatatgta
     5341 tggatcgtca agtacattcc tgtaacaaat aaaacaagaa caagcatacc aataaaaata
     5401 gatggctttt ttttaagcat atcgtcgtcc ccttatcaga agataaagga agatagcccc
     5461 accgatgatt ccaacgatca cactgactgg tatttcataa ggaaaaatca acacgcgagc
     5521 gacaagatcg caaaatacta agaacaaact acctgctaaa gcagttacgc caattgtttt
     5581 gtgcacttga tctccatata ttctagagac aagattcgga atgatcactc cgataaaagg
     5641 aatgcttcct accatgatca acatgactgc actagccaat gatactaagc caatgcctaa
     5701 aaactgcatc ttctggtaat ctattcccag atttactgca aacgattcgc ccagaccaat
     5761 gatcgtaaat cggtaagcaa atagccaaac caagagcaac aatggaacaa cagcatacaa
     5821 taactcataa tttcctcgta tcacagtcgc aaaattccct tgaagccatg aagtcatatt
     5881 ttgtaccaac tggaattgat aagcaaaaaa agtagcaact gaaccaatga tattccctaa
     5941 catgactcca atcaacggaa gcatcaattg atttttctgg aggaacaatc gcgtaaggga
     6001 taaaaaaagc aatgttcctg caaaagcaaa gcaaaatgct gtcaaggatc ggacaatcaa
     6061 ggatgcacca ggtaggaaaa tcatagccac taaaatacct aaacgcgcac tatccatcgt
     6121 gcccgcagtc gttggtgaaa caaatttatt ttgggtcaaa tgttgcatga tcaacccaca
     6181 aatactactg gtcgctccca caaggatcaa acaaatcgtt cgtggcaaac gagtcactag
     6241 caagatttgt aagctttgcc catctaactg gagcagttct gacaattgta cattcgatac
     6301 tccgatgaac aatgaaatac tacttaaaat aactaataat gctactagtc gtccgtagcg
     6361 catccaatcc acctttctaa ttgagaatga ttttcattct tattttaagt gaatcgttga
     6421 acaaaagcaa ccgctttttc atatgtttta ggtattattg ggaatatgct ataatcgtaa
     6481 gaaagacaaa gagaagaagg agggtatttt ttatggcaca agatgatgta aaaagccatt
     6541 tggataaggg actttatggt acacctttag tgaaccctga agaacagcat aaatatttag
     6601 ggacattcag ggaacgctgc tatcttagta tgactgttgc tgaaatgatc caaccaaaaa
     6661 acaaagaaaa ctttttaaaa gaactcaaaa aacatccaga tgcttccata ctcttgaatg
     6721 gggctctacc cctttctgtc caaaaccaat atatccagct agcaacgaaa aacaatagcc
     6781 gtttcactgt agtcaacgat tacgtcactg caaatcctga ggaattcgga ttgctcttaa
     6841 cagcaaaaga agcagtaaat gaacctgtga ttgatatcga aaaaaaatac ccagaagaaa
     6901 actcttcgcc agctgaaaaa gaaaaaccaa aaaagaaaaa aagcttctgg gatcgtttat
     6961 tttaaaacga aaaaacacat aaatttttac atgcaaaaaa agcttttgat cagattgaca
     7021 tcaaatagga caatctgtca aaagcttttt cttttaaaat acttcaggat ataacaattt
     7081 caatacattc atcgtcaatc ttctgccttt tcctgcaaaa ggataaacat gcatatgcat
     7141 ggctggattg aaatttgctt cgatgatccc atatgctttt ttatcagttg taggatcgat
     7201 ttctttatct ggtataatca aatcgatacc actaatctta gctcctagtg cttctaccgc
     7261 agaaacggcg atttttttgt aagcttcaga aaattcatca gtcatatcga tcgaatcacc
     7321 acctgtactg atatttgagt tctcccttag ataaacaatt tgattggctt ggggaactga
     7381 ttcgatcgtc aacccttgtt ctttcaacat caattgttct aattcaccta attggatcaa
     7441 ttctaaaggt gcacgatgat tcgttccacg caaagggtca ctattttttt cttcaacaag
     7501 ttctttcact gttcggatac tgtcaccaat aacattagca gggactcgca acatgatggc
     7561 ttggacttct ccgtcgatca caaagaaacg atattccgtt cccggcataa attcttctac
     7621 taaaaccgaa ctgtcttcac gaaaagcaat ggctaaacct gcttgataat cttccagact
     7681 agctccttcc ttgaaaatcg tgatacctaa cccgtaattg gttgatttag gtttgatcac
     7741 aaatgcctgc tcggcaaatc tcggataagc ttttaccgct tcttccatgg atgaaaattc
     7801 tgcgcctcct ggtacccgga acccagcttc ttttaagact tttttcgtta ctgttttgtt
     7861 ttccatgata agtgggacga tatagctgtc cttacttgtc atgtttgcat ttttcacata
     7921 ttctacatgg tcttggaatt gcagacgcaa aaattgatcc gactcatcta atactttgac
     7981 ttggacacct ttttggatgg catcgaacat aaatatctga gtagaaagtt ccatttcgcg
     8041 aaaaccggcc aactgatagg gacgttcatg gctttcttta taatattgat gtcccaatgc
     8101 cacagcaact tcttgctgac tcgatagctg tgcttcttga tagagtttcg ctgctaacgt
     8161 ttgttcagga tgatccattt gagtcaatgc ttcctctact aaataaagag aatccaacca
     8221 atccaattca accagcattt gcttcattcc ttcaagaatt tctctacctt cttgatgaaa
     8281 ttctgtttga tctgatggtt gttctagtgc tactttgtta tttcgagtcg taccttctgc
     8341 tacccattgg tcacagtctt ctttttcttc tgtccataac atgtacatca ggaataattg
     8401 taagaaccga acttgctctg gattgatacc caatcttgca taaggattca aatcgatatt
     8461 ccgcaattca atataacgaa cacctgttgt tgctaaatct gcgactttct ttccgcctcg
     8521 gaaacgaagc ggtgtataaa attctttctc ctccgaaagt ttcccttctt cgatcattcg
     8581 ctcgatatct gctaaatact gtttcataga agcataggaa actttcacat ttggatgatt
     8641 cgtatagccc agcgcactgt tgcgaatact gcgaacaggt tcttgcggat gtgattcatc
     8701 taaaaagtag ttcttctcac tcattggtga agcaccgaat aaataagtga tcatccagcg
     8761 atagcgcata aagtttctcg ctgttttcaa ataaagttct gttttgaatt cggaaaaatc
     8821 ttgaaattct tcctgatggc tgaacaatgt tcttagcaac tcatcgccaa attcaaaatt
     8881 gaaatgaata ccgcttacca tctgttttcg cttcccgtat tcttttgcta aataacgtcg
     8941 atacaagaca tcttcgaaat tttttaattt tgcaataatg atctcttcgt ctttttccgg
     9001 taaggcaggt ggcatactta atggccagat catctcttct ttcggtatag aacgagcagt
     9061 cacatcataa acagcagcga gatactgaaa taattcggga atagaatcgg taacaggggt
     9121 gatcatctct gtttgtgttt cgctaaaatc tgtttggata taggggtgat aggatcgatc
     9181 gccaaagatt gctggatgat cggtttttgc tagatttcct gcaagatcaa cccgctgtcc
     9241 ttcccgttct ataccaaatc gagcttggtc gataaaaggg cgcgcattga catgcaataa
     9301 taattgctta aaattcatca ttaagtgacc tccaattcat atgaatctaa ttataatcaa
     9361 aaagtctaag aaaaagaacg aaattgtttg agaaccgctt acaaataagt ataaaaatga
     9421 aggcatcaaa aacacagcta tgttaaaaac aatcattcaa acaaggagct gcaacttttg
     9481 tcacagctcc tgctttttag acatgggtta ttcttctttt agtaatcctt tgaacaatcc
     9541 aacgacaaaa tcatttggat caaacggctc caagtcatcg attccttcac caagaccgac
     9601 caatttcact ggtaaatgca attcattacg gatagcaagt acgataccgc cctttgctgt
     9661 accatctaat tttgtcaaaa caagaccggt cacatctgtt gtttctttga attgttttgc
     9721 ttgaaccatc gcgttttgac cagtggttgc atctaataca agaagtgttt cttggggtgc
     9781 ttctggtaat tcccgctgga ttactcgttt tattttttct aattcgttca tcaagttcac
     9841 tttattttgc agtcggcctg cagtatcaac tagtaaaaca tctgcctgct ctgtctttgc
     9901 tctttccaaa gcatcgaata cgacagctgc tggatcgcct cctgcattgc cacgaacaac
     9961 ttcaacacca gcgcgttcgc cccagacaac taactggtcg attgctccag cgcggaaggt
    10021 atctgctgct gccatcaata cttttttacc ttcgtttttg aactgatggg caagtttacc
    10081 aatacttgtt gttttcccta caccattaac accgacaaac aaaataactg ttagtccgtc
    10141 ttcttggata tttagttcat ttacttcatt gattccttcc gcttcataga gatccacaag
    10201 tttctcgatg atcgcatttt ggacttctgc cgattttttc gcatttctga gtttcacttc
    10261 ttgacgtaat gcttcggtaa ttcgtatcgc tgtttcaaaa cctacatctg caccgataag
    10321 tgtttcttct aattcttcaa aaaagtcttc atcgactgtt cgaaaattgg caaacaactc
    10381 attcatccgt tcaccaaatg ttttacgcgt cttttctaaa cccttgtcgt acttctcctg
    10441 tacttcaact tcttctgttt tctcttcgcc agtaaaagca cgcttgattt tatcaaaaaa
    10501 tcccatttga ctccccctac tctaatacaa atttttggat cactttggct acgccatttt
    10561 cttcattgct ggctgtcacg aaatctgctg cttctttgac gaaaggcaca gcattctcca
    10621 tcgctacgcc tagtccagca tattggatca tcggtaaatc attttcctca tcaccgatcg
    10681 ccatgatttc ttcttgtttc aaaccaagat cttttgccaa taaagaaata ccataagctt
    10741 ttgtaacacc ttttggcata aattccaaaa gcatacttct tgttttgatg atctcataca
    10801 tttcatggaa ttctgctggt atttctttga ttttaggatc taattcttct tggtcataag
    10861 cgattactgc cttattaagc acaaattcat ctgtaatatc gcttatatcc gctggttgga
    10921 attgcagcag cttattcaac acattgtaca aggattgctt ccttggggca ctaggcagct
    10981 gtaaaaccac attgtcagac aaaacatcta gtggcagatc caattgctga gccagtttgt
    11041 ataagcgttg gatatctgat acttccatca ctttcttttc catgatttca ccagtatcat
    11101 ttttttgtac caaaccgccg ttgaaagtga tgctgtagtc accttcatct accagtccta
    11161 attcttgcag ataatgcgcc atcgcagcta aggggcgtcc tgtacataga acgatcttga
    11221 ccccttgctg ttttgcttgt gctaatgctt gtttattctc atctgaaatc agcttttgtt
    11281 catttaataa tgtaccgtcc aaatcgattg ctactaattt gatcattttc gttcctacac
    11341 tttcgtttcg ttgattttcg cctcttcatt gatatcttcc atccgtacag aaacgatttt
    11401 tgatacaccg gattcctgca tcgtcactcc gtacagtaca tctgctgcga ccatcgttcc
    11461 tttgcggtgt gtgactacga taaattgtgt gtcatcttgg aaatcgctca agtaacgacc
    11521 aaaacgtttc acgttggctt catctaacgc tgcttctacc tcatctaaga tacaaaacgg
    11581 aacaggacag acacggataa tggaaaagag caacgcgatg gctgtaagtg ctcgttctcc
    11641 tccagaaagc aaacttagac tttgcagttt tttgccagga ggttgtactt caatctcaat
    11701 acctgttttc agcaaatcag ttggttccgt cagtactaat tcagctttac cgccgccaaa
    11761 catatttgga aagacaattt tgaattcttg acggatagct tcaaatactt ctttgaatct
    11821 tgtccgcact tcatcatcca tctcatccat cgtttcaaac aattggtttt tcgctgctaa
    11881 cagatcatct cgctgtgttg ctaagaatgt atgtctttca ctgacttgtt cgtattgttc
    11941 gattgaattt aagtttactg gtcccagttt ttcaatctgt tcttttaagg atgaaacctt
    12001 tgttctactg tcttcgatat cggttgtttc ttgatagtct tgggaagctt tctcaaatgt
    12061 cagctgatat tctgactgca gataggtcaa atgattatct aaaatcattt cagctcggtc
    12121 tttttggact tctaatttgg attgttccga cagtcgtgct ttttgctcac gattacgctc
    12181 tgccaagact gcttcaagct tatcgatctc cttttgcctt ctatctcgct gctctttggc
    12241 ttttgccaac tcggctttta atgtttctcg ctgagcagat aattcgttaa tttgtttttc
    12301 caaactttct tctgtgactt catgatcaga aaagtctgct gtcaatgtcg ccaactgctt
    12361 ttctatcgct tcttgacgtt cggttgcctc tgctttttgg actcgggcac cgcggagctg
    12421 gatctgcaga tgattaaact gctctttcaa gacagcaaga tctgcttgtt cttgtgcttt
    12481 ttgactttgt acttgtgctc ttctggcttc catctggtcg ctttcttgac ttaacgattt
    12541 gatttcttcg tcgatttttt gtcgttgcga ttcaagatct gtttgtttgt ctttcaattc
    12601 gttcgtttgc ttttcgtaat cttcgataaa ctgctgcagt tcacgtgtct caaaagttga
    12661 gatttgcttt tctttttcaa aacgttcaag ttcatttatg atgttttgta gctgatttgt
    12721 cgcttcttgt tcttcaaaac gtaattgttc gcctcgtgta cgcaaaactt cttgttcttc
    12781 ggctaaacga gctgtttctt gttgcagctc ctgaactttt ttttcttgtg cttggagttg
    12841 tttatctgct tcttcaaatt cactagtcaa ctgtttcaat tcttgatttt gtacaaacaa
    12901 actgccttgg tttcctcgtt tattcgctcc acctgtcatg gatcctccgg cattcatcac
    12961 atcaccttct aaggaaacca ctcgatattg atagcggatc gtttgcgcga ttgcattggc
    13021 actagttaag tctttggcta aaaggatcgt tcctagcagg ttttgaacca cagtttggat
    13081 ttgatcggga taactgactt gttcactggc aatccctata aacccttcta cagctgctgc
    13141 ttgtgtcaat atatgagccg gtaattgtct tggtttgatt gtcgtcagtg gaaggaatgt
    13201 tgctcttcct cctctttgct gtttcaaata cgtgatggct tgccgagcat ctttttcatt
    13261 ttcaacgatg acatgctggg ctgccccacc taaagctgtt tcgatagcta acgtgaaatc
    13321 tgcaggaaca tcgatcagtt cagccactgc tccgacaata cccgaaagct gctgtttatg
    13381 ctgcaagacc aatcgaaccc cttggtaaaa accaaaatag ttttcttgga tttcttgaag
    13441 gcttttctta cgagcacgta gctgctggac ttgattcatt aattgataca ttttaggctg
    13501 ttcttttgct aatgcttctt ggaacttttt cccgttttgt tggatcattt ctaacttctc
    13561 ttttgtttca gttaaagatg attttagctg cgcctgcttt tgtgtcaatt cttctttttg
    13621 taagatcaat cggtctactg atgcttctac ttccgattgc ttggctaacg tctgcttgct
    13681 ttttgctgtt tcttgtatat attgacgttc tagatacttc agttcgtttc ctacagctgc
    13741 ttgttcttgc atcaaatcaa catattgatc tcttaattct gccaataatt ctttcgttga
    13801 tttttggtat ttttctaatt cttgctgtgt ttttatgaga tttgcttcag ctttttggat
    13861 ctctgtttct ttttctgctg ctgctttcat caagctttct tgaagctttt caaaatgttt
    13921 aacctttttt tgtgcttctg ctaatgaagt ttgatattct tgcgagcttt tttgtgtatg
    13981 ctttgtccgc tcctgcaaaa catctttttg tccttctgtc tgcttcagtt tttccgaaag
    14041 atctaataat acttgctgat ttttttcgat cagacgatcg gcttgcgcat tttctttacg
    14101 ctgcttcgcc aaaatggatt cttgttcttg gatcgactca gatagtttgc ctaattctag
    14161 attgaatttc gcaagctgtg cttgtttgtt gtcccaatct ttttttgctg tcttgatttc
    14221 agcaaccatc aaagagacat cggtttgagt cagtgtttct tttagccgta aaaactcctt
    14281 tgctgcctca ctctgggcgg ccaaaggagt taactgttct tccaattcgt ggatgatgtc
    14341 ttggacgcgg ctgaggttgt cttctgtttc aaaaagcttc tgttctgctt tctttttccg
    14401 ctgcttgtat ttcaagacac cagctgcttc ttcaaagatc ccacgacggt cttccggttt
    14461 actactgaaa atcgcttcta cttttccttg agaaatgatt gagaacgatt cttttcctaa
    14521 tccagaatct aagaacaatt cttggatatc ttttaatctg cagctttgtt tattgatgaa
    14581 aaactcgctt tccccagtcc gtcgataacg gcgggttaca ctgatctcat tgtactccaa
    14641 tggcagataa tgatcactat tgtctaaaat cactgtgact tctgcaatat tcagctgctt
    14701 acgggtatcg gaaccggcaa agatgatatc cggcattttt ccgcctcgca gacttttagc
    14761 agattgttct cctaataccc agcggatagc ctctgtaata ttgctttttc cgctgccatt
    14821 tggtccaacc actgctgtga cgctattttc aaaatcaatg actgtcttgt ctgcaaatga
    14881 tttaaaaccg gctatttcaa ttcgtttcaa atacaaagct ttccctcact tacttttaca
    14941 gtgctgccaa tgcattttcc gccgcagctt gttctgctaa ttttttcgat ttcccttgtc
    15001 cagtgccaat caattgatca tctacataaa cttcgatcca aaatactcgt tcatgagctg
    15061 gaccttcttc attgatcaat cggtattcga ttgatacatc acctgatttt tgcaagactt
    15121 cttgtagttt tgttttatga tccatctcat gtgaaaaagc acctgctttg actttaggaa
    15181 aaatcacttt ttcgatgaat gcatgggtcg tgtcgaatcc ctggtcaaga tataacgcgc
    15241 ctaaaaaagc ttcgaatagg tcgcaaagca atgctggtcg cgtacgtcct cctgaattct
    15301 cttctccctt gcccaacaaa acatattgat caaagtgaca ttctttggca aatttcgaca
    15361 agctgtcttc gcgaacgatc gcagctcgtg tttttgtcaa ttttccttct ggcaaatgag
    15421 gatataaacg gaataaatac tgagaaacca tcagttctaa aacggcatct cctaaaaatt
    15481 caagtcgctc attatccgat aattgcagat tgcgatgctc attcacatag gatgaatggg
    15541 taaaagcttg ttcaagcaaa tttagatcgt gaaaaacgat gttatagttt tcttttagtt
    15601 cttttgtcag ctggttgtcc attttccaca ttccttttat ggatattttt tcgatacttc
    15661 cttattatac tggttttttg tcaaatttga aatggttcgg tcaaaaggta agtaaagctt
    15721 taataatcct agtgattgac gagaagttgt gaaaaattcg tttagtttcg agtatgaaga
    15781 aaaaattttg tgaaaatggc tttccatatt tttgcataaa tttgacttaa taccgagaaa
    15841 ctggactttg aacaccgttt attggagaag tcatgaaatc agcgccttga cctgaatatt
    15901 agagaaataa acagaaaaat atcaaaaaac cgaaaaagga catcgccctt tttcggtcat
    15961 ttttagtgat tatgaacgtg aatctgctgt aacagaaaca tttgcccatg ggtttggtac
    16021 atcataagac caatcccagt ctttgacacg gtcgttgact ggcaatactt cattacggaa
    16081 caacgttgga atcgcaaatg cttcttcaaa tgcatattgt tgccattttt gatatgcttc
    16141 tttttggtac tcttcatcaa atgctttgct tgaactgata tcgttgatca attttgtatt
    16201 ttcttctgat tcataacgag tgtagttgaa cgcagcattt ggtccccata gtccacttgg
    16261 atttggatca gttcctgtat tccaagcagc ttggtaaaca tcaatttcag gatcatcgtt
    16321 ttctaattta tcatagaaag aattgaattc gatcagacga cctgttgtgt attggacatc
    16381 caaaccaatt tctttccatt gttggatata gtaatctgcc aatggttgcg ctgtttcccc
    16441 accggacatc gaagcaaagt tgattgttag tttatttcca tctttgtctt ctcttaaacc
    16501 atcgtcatcc acatctttgt agccagcatc atctaatagt tttttcgctt tgtctagatc
    16561 ttgtgtgaat cctttcatct catcatcatg gtatgactgg aatactggag gaatcaatga
    16621 agtcgcattg cttctcaaac ctgcatagaa acgttcacct actgcattat tatctaatgc
    16681 atagcccatt gcttgacgta atgatttatc agccattttt gaatctggat tgtattccac
    16741 actctttgta tctgcattcc atttacctaa cttgaatcct agataagtat aagaagtttg
    16801 ttcacggcct aagtttgtat agccagcaat ttctttatag gtatcgtaag tatctgttgg
    16861 catttggaaa atcatatcat ataatttaga gttcaatgct tcagttgcac ttgccatcgg
    16921 tacagatttc atcgtgatct ttttcaattt aggcgtacca ttccagtagt gctcgtttgg
    16981 caagtattct actgattctc ctgcaacgat attactcatg taataagggc caaaagtcac
    17041 tggattttta cgtacttgat cgcttgattc catatctttg attgctacac cttctaacgt
    17101 atgttttggt gctgcatatg accagatacc accacccgat tgaagcatgc ttgggtttac
    17161 ttctttatat tcgatttcaa ctgttttgtc gtctattttt ttgattccgg agattgtgtc
    17221 ggcacttcca tcatgatatt cttccatacc tacgatgtta cggaaagtgt cgtcataacg
    17281 gataccagtg taatctttat tcccaatgat ttcgtaaggg aaaatcacat cgtccgccgt
    17341 tacatcttca ccatctgacc atttgacgtt gtcttttact ttgattgtcg cttttttcgt
    17401 atcttcatct agatcaagcg ttgcagctcc gtcgtctgtg attctgaaat cgtcatcgtt
    17461 tgcaaatagc ggttcatgag aaggagccat aaaagctgca tcatatgaat cttggtaaaa
    17521 ctcccactgg aacaatcctt taaactgact gtccgttgcg accgcagctt ctaattcgcc
    17581 gtcttcgatt gcatcatcat tattttttgt tgcaattgga aaagttaatt cctctttgtt
    17641 attgccgttg cctccagagt tgtttccaga ggtatctgta cctccgcccc cgcatgctgc
    17701 aagaacgaca gcagacatca gcgctgcaaa tccaaaaatc gttttcttca ttcctatttc
    17761 ccccttcgct tctatactag cctaaccgtt ggcgcgcatc tgcagaacgc ttgaatgctt
    17821 gtccaacata attgatactc aacatcaaaa ctaaaataag gattgatgcg ggtaaccaga
    17881 tccattgccg attcactaag acttctccgt tacttgcata accgatcaat gtacctaaac
    17941 taggtacatt tgccggcaag ccgaaaccta agaaagtcaa agttgtctcg ataccgatgt
    18001 ttgccgcaaa gttcatcgtc aaattagtaa tgattaatga acttagattc ggcatgattt
    18061 cgcggaacat gattttaaaa tcattggttc ccattgtttt tgaggcactg acataatctc
    18121 ttcttgcttc agataatgtc ttgcttcgga ataatcgggc tttagctacc caataaaatg
    18181 cactcatgat gaatataaag gaccagacat tgtatctgga aataatcgat acgaagacga
    18241 taatgatcag catgatcggt agaatcatga taaaatcaac gatccgcatc aagatgttgt
    18301 caatgatccc tccgtagtag ccagagatga tccctaatcc aaccccgatg atcgaagtaa
    18361 ggatcgtgat cgcaaatccg atcgtaacag agtttctagc acctatgatc aattgtccta
    18421 atacatcacg tccgccttca tctgctccca gaatgaactt ttgtccttgt gtcgaaactg
    18481 ttcctggtgc agcgtatttg tcgaagatac taacatacat cactttgtct tgatcggtca
    18541 gcatcgcacc gataaagatc actaataata aaaccactag caaacctaat gaaaaaatag
    18601 caattttatc ttttttaaat tctcgagcaa tcattcgtat ccccatagga ggaatacttt
    18661 cttgaactgc tgtattgttt ttttcactca tcgatccatt ctcctttctg attggtttta
    18721 ttgaatccga atacgcgggt caacgatact catgatgata tctgaaagta atgtccccac
    18781 tagcgtcgcg attcctaaaa tcaatactaa ggatgtgatc acggtatagt cccgttggcc
    18841 gatggaatca ataaatagtt ttccgactcc cggataacca aaaatctttt cgataactac
    18901 agaaccagca atcaacgaag tgatttcata ccccatttga gaagccatcg gcaacgctgc
    18961 atttcggaaa atatgtcgac tgaatacttt attcgtcggt acccctttgg aacgggctgt
    19021 tctgacaaaa tcgagcgatt tcgaatcgat tacttcactt cgcagatatt ggattgtgac
    19081 ggcagtcgcc aaaaatgctt gggtaattga tggtaacaga atatatttca aacggtctat
    19141 ccattgagtg ataaatccac cttgcaaacc tagtgtttgt gatccacttg ttgggaacca
    19201 gcctaaacga taaccaaaaa caaatagcat gatcaagccg aagataaata aaggaacagc
    19261 aaaactaaag aagttgtaga ttacgacaat cttatccaag attgaatttt gataccggcc
    19321 agcaagcatc cccagaggca cagcaatcaa ataagtaagg acaactgtca agatcgatag
    19381 atataaagtg ttgatgatcc gttcgccgat caagtcagca acagagcgtt tgtataagaa
    19441 actttgacca aaatcgcctt gcaaagcatt tccgatccaa cggaagtact ggacatacca
    19501 tgggtcattc aagccagctg cttgtcgcat ctgttcgatc actgccggat cctgatttgg
    19561 attgatcaaa ccggtaaatg gatcccccgg catcgcttgg gctaataaga agatcagaac
    19621 acttaaaata ataacttgcg ggatcatcaa taaaacacgt cgcagaatcg ttttccacat
    19681 tagcttgcac ctccatcttt caacgccact ttgtgtgtag ttgtgatcgt acgcaaatcg
    19741 tagactcgac cagtcgcatc atagtattct ttttggtttt ggatatactc ttgttctact
    19801 cttcgtcgat tttctttgtg gctttcccta ttttctacat cgatttgcgg aatagctgaa
    19861 agcagacgtt tcgtatagat atgtcgcggg tcattataaa tgtcttctct tgtaccgatt
    19921 tctacaaatc gacctttgta cataattgcg atattgtcgc acatgtgttt tactacacct
    19981 aaatcatgtg aaatgaataa atagctgagg ccgtattctt gctggattcg tttcatgaaa
    20041 ttcaatactt gcgcttgaac agatagatcc agagcagata caggttcatc ggctacgatc
    20101 agttttggat tggtagcaac agcacgggct actcctagac gttgacgttg tccgcctgaa
    20161 aattcatgag gatatttata tagcgcatct tccggcatcc caacgatatc tagcagtcct
    20221 tttacctttt tcttttcttc ttgatcgctt aaccgttcaa aatttcggat gggttcagca
    20281 atgatatcca acactcgctt tttaggattc aaactggaca ttgaatcttg gaagatcatc
    20341 tgcacatcct tgttataacc catttttttg cggttgcttc gcttcgttac atcttttcct
    20401 tcatacatga tttgtccggc tgttaccttt tctaatccca caacggcttt tcccgtagta
    20461 gattttccag agccagactc tcccactaaa ccatatgtct ttcctttttc gataataaaa
    20521 tccacaccat ctacagcata tacatgatcc gttactcgat tgaagaatcc actgcgaata
    20581 gggtaatgaa ctttcaggtc tttgattgtg attaattctg tcatctacac ctctcctttt
    20641 tcgtctctaa agtggaaatg cttatagcag gagcaacgta ccaagtggtt cggtgcgact
    20701 tcatgaagga taggctcttc ttcatgatcg cttgctggta tccatggaat acgtggtgca
    20761 aaccgacaac ctttacgagg catgtttttc agagaaggaa cgacaccttc gatcacatgg
    20821 agttctgcat catgatttcc ttcttgtggt atagagttca gtaaagagcg tgtataagga
    20881 tgcttaggat gatcaaacag ttcttttaca cctgctactt caacgaactg tccgccatac
    20941 atcactgcca ctcgatcagc catttctgct acgactccta agtcatgagt gatcaagatg
    21001 atccctgttt ttgtttcttc ctgaaggtct ttcagtaaat caagaatctg tgcttggatg
    21061 gttacatcta gggctgtcgt cggttcatct gcaatgacga ttggaggttt gcaggcgatt
    21121 gcgattgcga tgatgacacg ttgtctcata ccgcctgata gttcatgggg atattggcgt
    21181 ccaactctcg caggattagg aatccctact tgttctagca attctagtgc acgagcttga
    21241 cgctgttctg cattcatctt tgtgtgatag gaaagtcctt ctttgatctg gtcttcgatt
    21301 ctcatgagag gatttaatgc agacaacgga tcttggaaaa tcattccgat atcatttccg
    21361 cggattttat tatagagcgt ttcgttcagg tcgattaaat tcagatcgtt atataaaatc
    21421 tcacctgtaa tttttgtgtt attaggatca agtaatccca taatcgtagt agccaaggta
    21481 cttttcccgc acccagattc cccaacgatt gctaaaatct cgtttttgtc taaagtaaat
    21541 gaaacgtcat ctactgcatc gtaatagtca tctttgagtc ggaatcctgt atgtaggttc
    21601 tgaacttcta ataattggtt atcggacaat cgaatacccc tctctgcttc ctaaatggaa
    21661 gtttttctta ataaaaaagg acattacaca gaataaatcg ttttataaca aaggttattc
    21721 ttatctttgt
//
LOCUS       DAIGOK010000041        21209 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.041, whole genome shotgun sequence.
ACCESSION   DAIGOK010000041 DAIGOK010000000
VERSION     DAIGOK010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.041"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            262..570
                     /gene="rpsJ"
                     /locus_tag="NOA01_001206"
     CDS             262..570
                     /gene="rpsJ"
                     /locus_tag="NOA01_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCI0632413.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            601..1230
                     /gene="rplC"
                     /locus_tag="NOA01_001207"
     CDS             601..1230
                     /gene="rplC"
                     /locus_tag="NOA01_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCI0632414.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            1258..1881
                     /gene="rplD"
                     /locus_tag="NOA01_001208"
     CDS             1258..1881
                     /gene="rplD"
                     /locus_tag="NOA01_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCI0632415.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            1881..2171
                     /gene="rplW"
                     /locus_tag="NOA01_001209"
     CDS             1881..2171
                     /gene="rplW"
                     /locus_tag="NOA01_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCI0632416.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            2210..3043
                     /gene="rplB"
                     /locus_tag="NOA01_001210"
     CDS             2210..3043
                     /gene="rplB"
                     /locus_tag="NOA01_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCI0632417.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            3086..3364
                     /gene="rpsS"
                     /locus_tag="NOA01_001211"
     CDS             3086..3364
                     /gene="rpsS"
                     /locus_tag="NOA01_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCI0632418.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            3387..3734
                     /gene="rplV"
                     /locus_tag="NOA01_001212"
     CDS             3387..3734
                     /gene="rplV"
                     /locus_tag="NOA01_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCI0632419.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            3748..4404
                     /gene="rpsC"
                     /locus_tag="NOA01_001213"
     CDS             3748..4404
                     /gene="rpsC"
                     /locus_tag="NOA01_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCI0632420.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            4407..4841
                     /gene="rplP"
                     /locus_tag="NOA01_001214"
     CDS             4407..4841
                     /gene="rplP"
                     /locus_tag="NOA01_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCI0632421.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            4831..5019
                     /gene="rpmC"
                     /locus_tag="NOA01_001215"
     CDS             4831..5019
                     /gene="rpmC"
                     /locus_tag="NOA01_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCI0632422.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            5044..5310
                     /gene="rpsQ"
                     /locus_tag="NOA01_001216"
     CDS             5044..5310
                     /gene="rpsQ"
                     /locus_tag="NOA01_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCI0632423.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            5368..5736
                     /gene="rplN"
                     /locus_tag="NOA01_001217"
     CDS             5368..5736
                     /gene="rplN"
                     /locus_tag="NOA01_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCI0632424.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            5773..6081
                     /gene="rplX"
                     /locus_tag="NOA01_001218"
     CDS             5773..6081
                     /gene="rplX"
                     /locus_tag="NOA01_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCI0632425.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            6109..6648
                     /gene="rplE"
                     /locus_tag="NOA01_001219"
     CDS             6109..6648
                     /gene="rplE"
                     /locus_tag="NOA01_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCI0632426.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            6667..6852
                     /locus_tag="NOA01_001220"
     CDS             6667..6852
                     /locus_tag="NOA01_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HCI0632427.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            6888..7286
                     /gene="rpsH"
                     /locus_tag="NOA01_001221"
     CDS             6888..7286
                     /gene="rpsH"
                     /locus_tag="NOA01_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCI0632428.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            7318..7854
                     /gene="rplF"
                     /locus_tag="NOA01_001222"
     CDS             7318..7854
                     /gene="rplF"
                     /locus_tag="NOA01_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCI0632429.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            8015..8371
                     /gene="rplR"
                     /locus_tag="NOA01_001223"
     CDS             8015..8371
                     /gene="rplR"
                     /locus_tag="NOA01_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCI0632430.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            8392..8892
                     /gene="rpsE"
                     /locus_tag="NOA01_001224"
     CDS             8392..8892
                     /gene="rpsE"
                     /locus_tag="NOA01_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCI0632431.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            8907..9086
                     /gene="rpmD"
                     /locus_tag="NOA01_001225"
     CDS             8907..9086
                     /gene="rpmD"
                     /locus_tag="NOA01_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCI0632432.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            9130..9570
                     /gene="rplO"
                     /locus_tag="NOA01_001226"
     CDS             9130..9570
                     /gene="rplO"
                     /locus_tag="NOA01_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCI0632433.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            9570..10865
                     /gene="secY"
                     /locus_tag="NOA01_001227"
     CDS             9570..10865
                     /gene="secY"
                     /locus_tag="NOA01_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCI0632434.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            10927..11574
                     /locus_tag="NOA01_001228"
     CDS             10927..11574
                     /locus_tag="NOA01_001228"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCI0632435.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            11765..11983
                     /gene="infA"
                     /locus_tag="NOA01_001229"
     CDS             11765..11983
                     /gene="infA"
                     /locus_tag="NOA01_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCI0632436.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            12016..12132
                     /gene="rpmJ"
                     /locus_tag="NOA01_001230"
     CDS             12016..12132
                     /gene="rpmJ"
                     /locus_tag="NOA01_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCI0632437.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            12150..12515
                     /gene="rpsM"
                     /locus_tag="NOA01_001231"
     CDS             12150..12515
                     /gene="rpsM"
                     /locus_tag="NOA01_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCI0632438.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            12543..12932
                     /gene="rpsK"
                     /locus_tag="NOA01_001232"
     CDS             12543..12932
                     /gene="rpsK"
                     /locus_tag="NOA01_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCI0632439.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            13012..13950
                     /locus_tag="NOA01_001233"
     CDS             13012..13950
                     /locus_tag="NOA01_001233"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCI0632440.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            13978..14358
                     /gene="rplQ"
                     /locus_tag="NOA01_001234"
     CDS             13978..14358
                     /gene="rplQ"
                     /locus_tag="NOA01_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCI0632441.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            14630..15469
                     /locus_tag="NOA01_001235"
     CDS             14630..15469
                     /locus_tag="NOA01_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCI0632442.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            15445..16314
                     /locus_tag="NOA01_001236"
     CDS             15445..16314
                     /locus_tag="NOA01_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCI0632443.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            16304..17101
                     /locus_tag="NOA01_001237"
     CDS             16304..17101
                     /locus_tag="NOA01_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCI0632444.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            17304..18050
                     /gene="truA"
                     /locus_tag="NOA01_001238"
     CDS             17304..18050
                     /gene="truA"
                     /locus_tag="NOA01_001238"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCI0632445.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(18383..21052)
                     /locus_tag="NOA01_001239"
     CDS             complement(18383..21052)
                     /locus_tag="NOA01_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HCI0632446.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT         7056 a         3871 c         4564 g         5718 t
ORIGIN      
        1 aaaagagtca gtattttcta aatgaaactt tatgaaaaca aaatgttttt tacaagaaaa
       61 agccttgctt tgtgagttta tatatattat aatgacaaag gtgcgtttac tttagtgaat
      121 tgcacagacg taatcagaat gatttcgtcg gctaagaaac gagaggttgc gacacgcccg
      181 gacgctttgc cacgaacgag cgtgacggaa attttcgtgg agctatgtct acttttcaaa
      241 ataggcgaag gagggaacaa gatggcaaaa caaaaaattc gtatccgttt aaaagcgtat
      301 gaacaccgta ttttagatca atcagcggat aaaatcgtgg aaactgcaaa aagaactgga
      361 gctagcgtgt ctggtccaat tccattacca actgaacgca gtctttacac aatcatccgt
      421 gcgactcata aatacaaaga ttcacgcgaa caattcgaaa tgcgtacaca caaacgtctt
      481 atcgacattg tgaacccaac accaaaaact gttgatgctt tgatgaagct agacttacca
      541 tctggtgtaa acatcgaaat caaattataa aactaaaaca aaaataatgg aggtgtactc
      601 atgaccaaag gaatcttagg gaaaaaagtg ggaatgacac aaatcttcac tgaatctggt
      661 gaattaattc cagtaactgt tgtagaagct actccaaacg tagtactaca agtgaaaaca
      721 atggaaacgg atggctacga agctattcaa gttggttacc aagatatgcg tgaagtttta
      781 tcaaacaaac ctgcgaaagg tcatgttgca aaagcaaaca cggctcctaa gcgcttcatt
      841 cgtgaattca aaaatgttga gctaggagaa tatgaagtag gaaaagaaat tacagtagat
      901 gttttccaag caggagacat tattgatgta acaggtacta cgaaaggtaa aggattccaa
      961 ggggttatca aacgccacgg acaaagccgc ggaccaatgg ctcacggttc tcgttaccat
     1021 cgtcgtcctg ggtcaatggg tccagttgca cctaaccgtg tatttaaaaa caaacgtctt
     1081 gctggccgta tgggtggcaa ccgcgtaact atccaaaact tggaagttgt acgtgtcgat
     1141 gcagaaagaa atgttatctt gatcaaaggt aatgttcctg gagctaaaaa atctttaatt
     1201 actatcaaat cagctgtgaa agctaaataa ttgtaggaag aaaggaggaa ctaaggaatg
     1261 ccgaatgtag cattattcaa acaagatgga agccaaaacg gtgaaatcac acttaacgaa
     1321 gaaatcttcg gaattgaacc taatgaaagt gttgtttacg atgcaatcgt aatgcaacgt
     1381 gcgtcattaa gacaaggaac acacgcggtt aaaaaccgta gcgctgttcg cggcggcgga
     1441 cgtaaaccat ggcgtcaaaa aggaacaggt cgtgcacgtc aaggatctat ccgctcacca
     1501 caatggcgtg gaggtggcgt agtctttgga ccaacaccac gttcttacag ctacaaactt
     1561 cctaaaaaag ttcgtcgttt agcaatgaaa tctgtattgt cagaaaaagt tgctgaaaac
     1621 aacttggtag caatcgaagg cttgaacttc gacgcaccaa aaacaaaaga attcaaacaa
     1681 gttcttgcta acttatctat cgactctaaa gtattggttg ttctagaaaa aggtaacgac
     1741 tttgctgctt tatcagcacg taacttacca aacgtttctg tagtaacttc tgataatgtg
     1801 agtgtgctag acgttgtatc aaatacaaaa gtgttagcaa cacaaactgc tcttactcaa
     1861 attgaggagg tgcttgcata atgaacttac tagacgtaat caaacgccca gtgatcactg
     1921 aaaaatcaat gcttgccatg gatgacaaga aatacacttt tgaagtggac actcgcgcaa
     1981 acaaaacttt agtaaaacaa gcggtagaag ctgcttttga cgttaaagtc aaaaacgtga
     2041 acatcgtgaa cgtacgtgcg aaattcaaac gtatgggcaa atatgcagga tatacaaaaa
     2101 aacgtcgcaa agcgatcgtt acattgactg aagattcaaa agaaatccaa ttattcgaag
     2161 ctgctgaata agcactaata gataactcat taaattagga gggaaaaacg tggcgattaa
     2221 aaagtacaaa cctacctcaa atggtcgtcg taacatgaca ggttctgatt ttgctgaaat
     2281 cacaacatca acacctgaaa aatcattgtt acaaccattg aaaaaccatg ctggacgtaa
     2341 caacaacggc cgcatcactg tacgtcatca aggtggcgga cacaaacgcc aatatcgcgt
     2401 gatcgacttc aaacgtaaca aagataacgt tgttgcaact gttcaaacga tcgaatatga
     2461 tccaaaccgt tcagctaaca tcgcgttagt acactatgaa gatggagtga aagcttacat
     2521 cttagcacct aaaggactag aagtgggtat gaaacttgtt tcaggtccag aagcagatat
     2581 caaagtcgga aatgctcttc cattagaaaa cattccagtt ggtactgtta tccacaacat
     2641 tgaaatgaaa cctggtaaag gtggacaatt gatccgttct gctggtacaa gtgctcaagt
     2701 acttggtaaa gaaggcaaat acgtattgat ccgcttgaac tctggtgaag ttcgtatgat
     2761 cttagctgca tgccgtgcta caatcggttc tgtaggtaac gaacaacacg aattaatcaa
     2821 catcggtaaa gctggacgct ctcgttggat gcgtaaacgc ccaactgtac gtggtagcgt
     2881 aatgaaccct aatgatcacc cacacggtgg tggtgaaggt aaagctccta tcggacgtaa
     2941 agcaccagtt tctccttggg gtcaaccagc tcttggttac aaaactcgta acaaaaaagc
     3001 taaatcagac aaacttattg ttcgtcgtcg caaaaataaa taattacttt gaccatgtgt
     3061 cgcacatttt gagaggaggt tcaccatggg tcgtagttta aagaaaggac cttttgtcga
     3121 tgagcattta atgaaaaaag ttgaagctca agcaggggtt gaaaagaaaa aagttatcaa
     3181 aacttggtca cgccgttcaa cagttttccc aagttttgtt ggatatacca tcgcagttta
     3241 tgatggacgt aaacatgtac cagtttacat ccaagaagac atggtaggac acaaattagg
     3301 tgaatttgca cctacaagaa cttatcgtgg acatgctgct gacgataaga aaacgaaacg
     3361 ctaatttgag gggaggataa atccaaatgg cagaacaaat cacatcagct aaagcaactg
     3421 caaaaacagt tcgcacttca cctcgaaaag cccgtttagt aattgacctt atcagaggta
     3481 aaagtgtcgc agatgcaatt tcaatcttga aattcacacc aaacaaatct gctggaatca
     3541 ttgaaaaagt attgatgtca gcagttgcga atgcagaaaa taactttgac ttagatgttg
     3601 aaaacttggt agtaagcgaa gcttttgtta acgaaggacc tacaatgaaa cgtttccgtc
     3661 ctcgtgcaaa aggatcagct tctccaatca acaaacgtac aagtcatatc acggtagtcg
     3721 tatcagaaaa ataaggaggg ataatcagtg ggtcaaaaag tacatccaat tggaatgcgt
     3781 gtaggcatca tccgcgactg ggatgctaaa tggtatgctg aaaaagagta tgctgagttc
     3841 ttacacgaag atttaagaat ccgtaaattt atttcaacta aacttgctga tgctgctgta
     3901 tcaacagttg aaatcgaacg cgcaacaggt cgcgtaaaca tttcaattca cacagctaaa
     3961 ccaggtatgg ttattggtaa aggcggttct gaagtcgaaa gcctaagaaa agaattaaac
     4021 aaattaactg gtaaaagaat ccatatcaat atcgtggaaa tcaaaaaacc agatctagat
     4081 gcaaaattag taggtgaagg aatcgcacgt caattagaaa accgtgttgc tttccgtcgc
     4141 gctcaaaaac aagccatcca acgctcaatg cgtgctggcg ctcaagggat caaaactcaa
     4201 gtatctggtc gtctaaacgg agcagatatc gctcgttcag aaggatactc agaaggaact
     4261 gttcctcttc atactttacg tgcggacatt gattacgcat gggaagaagc agatacaaca
     4321 tacggaaaac taggagttaa agtgtggatc tatcgtggag aaattcttcc aactaaaaaa
     4381 aacactgaga aaggagggaa ataatcatgt tagtacctaa acgtgtaaaa caccgtcgtg
     4441 aattccgcgg aaaaatgcgc ggggaagcta aaggcggaaa agaagtagca ttcggtgaat
     4501 acggtttgca agctgttgat tcacattgga tcacaaaccg ccaaatcgaa gctgctcgta
     4561 tcgcaatgac tcgttacatg aaacgtggtg ggaaagtatg gattaaaatt ttccctcaca
     4621 aatcttatac tgccaaagca attggggtac gtatgggttc tggtaaaggg gcacctgaag
     4681 gatgggttgc accagtaaaa cgtggtaaaa tcatgtttga aatcgcaggc gttcctgaag
     4741 aagtagctcg tgaagcgtta cgtctagctt ctcacaaatt accaatgaaa actaagatcg
     4801 taaaacgtga ggaaatgggt ggtgaatcga atgaaggtta aagaaatcag agaattaacc
     4861 actgccgaaa tgcttgatca agaaaaacaa ttaaaagaag aattgtttaa tcttagattc
     4921 caattagcaa caggtcaatt agaaaacact gcacgtatta aagaagtacg taaatcgatt
     4981 gcacgcatca aaactgtgtt gcgtgaacaa gctaagtaat tatggaagga ggccattaag
     5041 cgtatgactg aaggaagaaa tcaacgcaaa gtttatcaag gtcgcgtggt ttcagacaaa
     5101 atggataaaa caattaccgt tgctgtcgaa acaaagaaaa accaccctat ctatggcaaa
     5161 cgcatgaact attctaagaa atacaaagtg catgatgaaa acaacacagc aaaagttggc
     5221 gacatcgtga gaattatgga aactcgtcca ttatcagcta caaaacgttt ccgtttgttg
     5281 gaagtagtcg aagaagcagt tattatctaa taacacgaga ttttcctata tagattgaat
     5341 gcaaaatctg aaaggaggat acacaacgtg atccaagcag aaagtcgttt aaaagtcgct
     5401 gataactcag gcgcacgtga aattttgacg atcaaagtcc ttggtggatc tggtcgtaaa
     5461 actgctaaca tcggtgatgt gatcgttgct acggttaaac aagcaacgcc aggtggggtt
     5521 gtcaaaaaag gtgacgtcgt taaagccgtt atcgttcgaa caaaatcagg agctcgtcgt
     5581 acagacggtt catatattaa atttgatgaa aatgctgcag tgattattcg tgatgataaa
     5641 agccctcgtg gaacacgtat ctttgggcca gttgcacgtg aactacgtga aaacaacttc
     5701 atgaagatcg tttcactagc accagaagtt ttataatctt gagacactta tcaaaggagg
     5761 tgcgaaacag ttatgtttgt taaaaaaggc gataaagtta aagttatcac tggaaaagac
     5821 aaaaacaaag aaggcgttgt actagcagcg ttcccgaaaa aagataaagt tattgtcgaa
     5881 ggtgtaaatg tcgttaaaaa acaccaaaaa ccttctcaag ctgctcctca aggtggaatt
     5941 gttgagatgg aagcgccaat tcatgtttct aacgtgatgg tggttgactc tactggtgta
     6001 gctggccgag ttggctacaa agaagtagac ggcaaaaaag tccgtgtttc taaaaaaacc
     6061 ggtgaagttt tagataaata aaagtttaag gaaggagggt cttactgaat gaaccgccta
     6121 aaagaaaaat atcttaaaga agtaactcca tcattgatgg aaaaatttaa ctatagctct
     6181 gttatgcaaa cacctaaagt tgaaaaaatc gtcatcaaca tgggtgttgg tgatgcggta
     6241 tcaaacgcta aaaacttaga taaagcagtt gaagaattaa ctttgatcac tggtcaaaaa
     6301 ccaatgatta caaaagctaa aaaatcaatc gctggattcc gtttacgtga aggaatgcca
     6361 attggtgcaa aagttacatt gcgcggagaa agaatgtacg aattcttaga caaattggtt
     6421 tctgtttcac taccacgtgt acgtgacttc catggtgtaa gtaaaaaagc ctttgacggt
     6481 cgtggaaact atactttagg tattaaagaa caattgatct tcccagaagt agattatgat
     6541 ttagtagata aagtacgtgg tatggacatc gttattgtaa caacagcaaa ctctgatgaa
     6601 gaatctcgtg agttgttgac acaattaggc atgccattcc aaaaataatt aaaggaggcg
     6661 aactacgtgg ctaaaaaatc aatgattgct aaaaacaaac gtcctgcaaa attttcaaca
     6721 caagcttata ctcgttgcga acgttgcgga cgtccacatt cagtttatcg taagttccat
     6781 ctttgccgta tttgcttccg cgaacttgcc tataaaggtc aaattcccgg cgtgaagaaa
     6841 gctagctggt aaaaagtaaa ttcgcataag aaggaggtaa aagatcaatg gtcatgacag
     6901 atccaattgc agattttcta actcgtatcc gtaacgcaaa catggttaaa catgagtctt
     6961 tagaagtacc tgcatcaaaa attaaacgtg acatcgctga aatcttgaaa cgtgaaggtt
     7021 tcgttcgtga tgttgaatac atcgaagatg acaaacaagg cgtgatccgt gttttcctaa
     7081 aatttggtaa aaacggcgaa cgtgttatca caaacttaaa acgtatctct aaaccaggtt
     7141 tgcgtgctta tgtcaaagct gacgaagtac caaaagtatt gaacggttta ggtatcgcaa
     7201 tcatttcgac ttctgaaggt gttgtcactg ataaagaagc tcgtgaaaaa aatatcggcg
     7261 gcgaagttat cgcttacgta tggtaatcaa gtagaatcta caaggaggtg tctttaagtg
     7321 agccgtatcg gaaataaagt cgttgttatc cctgaaggag taacagttac tcaagacgga
     7381 aacaacgtta cagttaaagg accaaaaggt gaattgactc gtactttttc agcagatatc
     7441 aaaatgaata tcgaaggaaa cgaagtaact ttcactcgtc caaacgacag taaagaaatg
     7501 aaaacaattc atggaacaac tcgtgcgaac ttcaacaaca tggttgtagg tgtcagcgaa
     7561 ggtttccaaa aagcgttaga actaatcggg gttgggtacc gtgcacaatt acaaggatca
     7621 aaacttgtat tgaacgttgg ttactcacat ccagtagaaa tcacaccacc agctggagta
     7681 actgttgaag taccagctaa cacacaagta attgttaaag gtgcgaacaa agaagaagtt
     7741 ggcgaattag ctgcaaacat tcgtggaact cgtcctccag aaccttataa aggtaaaggt
     7801 attcgttatg ttggcgaata tgtacgccgt aaagaaggta aaactggtaa ataatcgttt
     7861 agaaaaattg catagaagcc agcagcagga aaaaggcatt tggctgaagt ttaccttcaa
     7921 aaaaatacat ttttacttgt gccgcagctt ctatttttcg aaacgtattt actgcttgaa
     7981 atagtagtac aataaaaata aagaggtgac aattgtgatt acaaaaccag ataagaacaa
     8041 aacacgtcaa aagagacatc gtcgtgtacg taacaatatc tctggtactg ctgagcgccc
     8101 acgcttgaac gtttttcgtt ctaacaaaaa catctacgct caaattattg atgacgtagc
     8161 gggtgtaacg ctagcaagtg cctctacctt ggataaagaa atttcaggtg gaacaaaaac
     8221 agaagcagca caagctgtcg gtaaactagt tgctgaacgt gcagctgaaa aaggcattaa
     8281 agtagtagtc tttgaccgtg gtggatacct ttaccatggc cgtgtgcaag ctttagctga
     8341 agctgctcgc gaaaatggac tagaatttta gaagaaggag gaataccatt catggtttat
     8401 atcgatccaa aacatttgga attagaagac cgcgttgttg caattaaccg tgtaacaaaa
     8461 gttgttaaag gtggacgtcg tctacgtttt gctgctttag ttgttgtcgg tgacaaaaac
     8521 ggacacgtag ggtttggtac aggtaaagct caagaagttc ctgaagcaat ccgtaaagca
     8581 attgaagatg cgaagaaaaa cttagttgaa gtacctatgg ttggttcaac aatcccacac
     8641 gaagtaatcg gtgcttttag tggtggtaga atcctaatga aacctgctgt agaaggttct
     8701 ggggttgctg ctggtggacc agttcgtgcc gtattagaat tagctggggt agcagatatc
     8761 acttctaaat ctttaggctc aaatacacca atcaacgttg ttcgcgcaac agttgaaggt
     8821 ttgaaacaat tgaaacgtgc agaagaagtt gcagaacttc gcggcaaatc agttgaagaa
     8881 ttaatcggat aaggaggaca acaataatgg ctgaattaaa agttacttta aaacgcagta
     8941 ttatcggacg tcctcaaaac caacgcgata cagttaaagc gttaggtctt acaaagatta
     9001 acagttcagt ggttaagcct tctaatgaag caattaaagg catgatcaat actgtttctc
     9061 acctagtgga cgttgaagaa atttaattta gaaaaatcat aactagaaca ttaaggaggt
     9121 gccaaaccta tgaaacttca tgaattaaaa cctgctgaag gttcacgcca agtacgtaac
     9181 cgtgttggtc gtggtacttc atctggtaac ggaaaaactg ccggacgcgg acaaaaagga
     9241 caaaaagctc gttcaggcgg cggtgtgcgt ttaggatttg aagggggaca aacaccattg
     9301 ttccgtcgtt taccaaaacg aggattcaca aacgtgaacc gtaaagacta tgcagtcatt
     9361 aatcttgatg tattgaaccg ctttgaagac ggagctgaag taactcctgt tgctttagtt
     9421 gaagctggaa tcgtgaaaaa cgaaaaagct ggaatcaaag tattagctaa tggggaacta
     9481 aacaagaaat taactgtgaa agcagcaaaa ttctctaaag cagctcaaga agcaattgaa
     9541 gcggctggtg gatcaatcga ggtgatctaa tgtttagact attaaaagat gcttttaaag
     9601 taaaagacat tagatcgaaa atcctattta ctgtatttgt gttgtttgta tttcgcctag
     9661 gtgcacatat tactgtacca ggcgtaaatg ctgcttcatt gcaaaacctt tcttctttgc
     9721 catttttcaa catgttgaac ttggtaagtg gtagtgcgat gcaaaacttt tccattttct
     9781 caatgggggt ttcaccatac attactgctt ctatcgtcat tcagcttctt caaatggaca
     9841 tcgtgcctcg atttgttgaa tggtcgaaac aaggggaagt tggtcgtaaa aaactgaatc
     9901 aagcgactcg atatctgaca ttagtattgg catttatcca atctgtcggt atcactgctg
     9961 gattcaatca atacgctcaa ttaggtttcg tgaataatcc taacgtagca acttacgtaa
    10021 cgatcggttt gattctaaca gccggtacga tgtttgttac ttggctagga gaacagatca
    10081 cagaaaaagg tatcggaaat ggcgtatcaa tgatcatttt tgcaggtatc atttctagat
    10141 tgcctgaaag tatcagagaa atttatgaag attactttat aaatattgat ccttcagaca
    10201 tatggaaatc tgctattttt gtggccattt tactagtagc ggtcttgttg atcgtgacat
    10261 tcgtaacatt tttccaacaa gctgaacgca agatcccgat ccaatatacg aaacgcgtag
    10321 ctggcgcgcc tacaagcagc tatctgccgt tgaaagtaaa tgcagctgga gttattccag
    10381 ttatctttgc tagttcgttt attgctacgc caaatgctgt tttacaagca ttgagcggta
    10441 gattcggttc tgaaaactgg tacaaagtga tgatggagat ctttaactat aatacagcac
    10501 ctggtgctac gatttatacg attctgatcg ttgccttcac tttcttctac gcctttgttc
    10561 aagtaaaccc tgagaaatta gcggaaaact tgcaaaagca aggaagttat ataccaagcg
    10621 tacgccctgg taaggggact gaagagtatg tctcaagatt actgatgcgt ttaagtacag
    10681 ttggtgcagt cttccttggt ttagtggcat tgcttccaat cattgctcaa atggtttggg
    10741 atcttccaca atcaatcgga ttaggcggta caagcttact gatcgttatc ggtgtagctt
    10801 tagaaactgc gaaacagtta gaaggcttaa tgctgaaacg taaatatgtt ggattcattg
    10861 agtaagaaag cgtgttgagg tttcccctca acacacgttc tcacacattt attaggaggg
    10921 aaaacaatga acctcatttt aatggggctg ccaggcgcag gtaaaggaac acaagctgaa
    10981 aaaatcattg atgcttatgg tattccgcac atctcaacag gagatatgtt ccgcgcagcg
    11041 atgcaaaacg aaacagctct tggcctggaa gcaaagtctt atatggataa aggtgcatta
    11101 gttcctgatg aagtaacaaa tggaatcgtg aaagagcgac tagctgaacc agatacagag
    11161 aaaggcttct tattagatgg ctttccacgt acgctagatc aagcaaaagc tctggacgca
    11221 atgctaaaag atttgaataa aaaaattgat gctgtcatcg atatccatgt tggtgaagaa
    11281 atcttggtcg aacgtctagc cggccgattt atctgcagca attgtggagc aacttaccat
    11341 aaaattttca accctacaaa agttgaagac acatgtgatc gttgcggtgg gcatgaattc
    11401 tatcaaagag aagatgataa acctgagacg gttaaaaatc gtctggctat caacatcaaa
    11461 aatagtgaac caatcttggc ttattataaa gaacaaggtt tgctaaacac tattgatggt
    11521 gctcgtgaaa tcgatgctgt atttgcagat gtacaaaaaa tcattgaaaa atagcacgat
    11581 tgcccgtagc cgacttgcat tatgcagtta ctcttgtcaa gttcaataaa ttatgataga
    11641 atatgtcagt ccgacttcta gagaatcctc tagatgatcc attttgagaa ttttgaggtg
    11701 ggaactgcag tttccgccat tttatcgtgt gaaaatgcga aacaagtaca aggaggtact
    11761 gtgcgtggca aaagaagata tgattgaagt cgaaggtaca gtcgtcgaaa ctttgccgaa
    11821 tgcaatgttt aaagttgaac tagaaaacgg acaccaagtt cttgctactg tttcaggtaa
    11881 aattcgtatg cattacatcc gtatcttacc tggtgacaaa gtaacggttg aattgtctcc
    11941 atacgatctt aaccgtggcc gtattactta tcgctttaaa taattgtact ccgtaaaact
    12001 acaggaggta ttatcatgaa agtaagacca tcagtaaaac caatgtgcga acattgtaaa
    12061 gtaattcgtc gtaaaggacg cgttatggtg atttgcccag caaatccaaa acataaacaa
    12121 cgtcaaggat aattggaggt gctataaata tggctcgtat tgcaggagta gatattcctc
    12181 gtgataaacg tgtagtagtt tcacttactt atatctatgg tattggtaac actactgcta
    12241 aaaagatctt agctgatgct ggtgtatctg aagatgttcg cgttcgtgaa ttaacgaatg
    12301 aacaaacaga tgctatccgt gcagaagtag ataaattaaa agttgaaggg gatcttcgtc
    12361 gtgaagtgaa cttgaacatc aaacgtttga tggaaatcgg ttcttaccgt ggaatccgtc
    12421 atcgccgtgg attgcctgtt cgtggacaaa acacgaaaaa caatgcacgt actcgtaaag
    12481 gcccagctcg cgctatcgct ggcaagaaaa aataattctt aagtgaagga ggttaaaact
    12541 tcatggcagc aaaaaaagtg aatcgtaaac gccgtgtgaa gaagaatata gaagcaggta
    12601 ttgcacatat ccattctaca ttcaataaca ctatcatcat gatcactgat actcacggaa
    12661 atgcattagc gtggtcatca gctggttcat taggtttcaa aggaagcaaa aaatcaactc
    12721 cttttgccgc tcaaatggca gcagaaactg ctacaaaagc agcaatggaa cacggattaa
    12781 agactgttga agtaacagtt aaaggacctg gttctggacg tgaagcagca attcgttcat
    12841 tacaagctac aggtttagaa gtgactgcaa ttcgtgacgt gactccagtt cctcataatg
    12901 gtgcccgccc tccaaaacgc cgtcgtgttt aatgagtgcc gctcattttg agttttatca
    12961 gtaacgtcat tgaacaagac acttttcgtt ttgaaagggg taaagataag aatgattgag
    13021 ttcgaaaaac caagaatcgc aaaaattgat gaagaaaaag attatggcaa gttcatcgtt
    13081 gaacctcttg aaagagggta tgggactact ttagggaatt ccctacgtcg tattttatta
    13141 tcttctttgc caggtgctgc catcactaat attcaaatcg atggtgtact gcatgaattc
    13201 tctaccatca aaggtgttag agaagacgtc actcaaatca tcttgaacat taaaggctta
    13261 gcgctgaaaa tgtatggcca agaagaaaaa acccttgaaa tcgatattac cggacctgct
    13321 acagtaactg ctggcgatat tatcgttgat agtgatgtag aaattcttaa caaagatatg
    13381 tacatctgta gtgtctctga aggagctacg ttccatgctc gcttaacagt gaaacctgga
    13441 cgtggttatg ttcaagcaga tgaaaataaa aaagaagata tgccaattgg cgttcttcca
    13501 gtcgattcaa tctacacacc tgttcgtcgt gtgaactatc aagttgagaa cacacgtgtt
    13561 ggacaccgtg aagattttga taaattgacg atggagattt ggacagatgg ttcaattgaa
    13621 ccgttggaag caatgagttt gtctgcgaaa atcatgactg aacatttgga tatttttgtg
    13681 aacttaactg atgaagcaaa aaatgctgaa atcatggtcg aaaaagaaga aacacaaaaa
    13741 gaaaaaatgt tggaaatgac gatcgaagag ttagacttgt ctgttcgctc ttacaattgt
    13801 ttgaaacgtg caggaatcaa tactgtacaa gaacttacaa ataaatctga gcctgaaatg
    13861 atcaaagtcc gtaacttagg acgtaaatca ttagaagaag ttaaagcaaa attgcacgac
    13921 ttaggtttag gtttacgtaa agaagattaa aaccttttac gaaggaggga aaccaacgtg
    13981 agttatcgta aactaggacg cacatctagc caacgtaaag cgatgttgcg tgatttaaca
    14041 actgatttat taatcaacga acgtattgtg actactgaag ctcgtgcgaa agaagttcgc
    14101 tcaactgcag aaaaaatgat cactttaggt aaacgcggag atttgcatgc acgtcgtcaa
    14161 gctgcggctt tcgtacgtaa tgaagttgcc agcgtgcgtg aagaaaatga ggacatcgtc
    14221 attgaatcag ctttacaaaa actgttcaac gatattgcac ctcgttacgc agaacgtcaa
    14281 ggcggataca cacgcatttt gaagacagaa ccaagacgcg gtgatggtgc accaatggtt
    14341 gtcttagaac ttgtctaaaa tcacactaat aacatcactt tatagatgat tcttttagag
    14401 tgttatgatg ttggaagata tttttccgag tctagctcag cgctgctcct caagcgaata
    14461 tcgcttgggg agtaagcact catcataaag tgttttttta taccttcaat tacctgtcat
    14521 gacagatatg tttgaaggca taaaaaaaca ttttaagcga gaaataattg aaaattttgt
    14581 ctttttttga tagtgttagt aggaacttta ggatgaagaa ggagtaatca tggagccaat
    14641 tattgaatta gaaaagataa attataaata ccagccagat gatcttcgcc ccgcattgaa
    14701 agatgtctcc tttacaattg ataaaggtga gtggatcgct attattggac acaatggttc
    14761 aggaaaatcg acacttgcca aaacgatcaa tggattgctt ctgccagaat cagggatcgt
    14821 gaaagtggga aatcaaatat tggatgaaga aaatatttgg acgatccgac agatggttgg
    14881 aatgattttt caaaatccag acaatcagtt tgttggttcg acagtagaag atgacgttgc
    14941 ttttggtctt gaaaatcaag ggattcccag agaagaaatg ctagtgcgtg tgaaagatgc
    15001 acttgagaaa gttagaatgg ctgagtttgc ttcacgcgag cctgctcgtt tatcaggagg
    15061 gcaaaaacaa cgtgttgcca ttgccggcgt ggttgcctta cgacctgata tcattatttt
    15121 agatgaagca accagtatgc tagatccaga aggtcgtgaa gaagtgatct ctacgatcaa
    15181 gaagatcaaa gaagaaagtc aattgaccgt tatatctatc actcatgata ttgatgaagc
    15241 agctaatgcc aatcggatac tagtgatgag acaaggcgaa cttgtccgtg aaggtacacc
    15301 aaaagaaatt ttttctgctg gtcctgaact gatcgactta ggtctagatc taccatttcc
    15361 tgaaaaactg aagagcgcac tgaaagaacg tggggttgat gtgcccagtg aatatatgac
    15421 agaagaaagg atggtggatt ggttatggac atccgttttg aacaagtaga cttcacttat
    15481 cagccgaata caccatttga acaaagagcc ttatttgaca tcaatatgac gatcaaagaa
    15541 aacagttata ctgcattagt agggcatacc ggaagcggga aatcaacctt acttcagcat
    15601 ttgaatgcgc ttgttaagcc aacaagcggt acagtccata ttggagaacg agatattcag
    15661 ccggatacgg ataataagaa tttaaagccc atccgaaaaa aagttggcat cgtctttcag
    15721 tttccagaag cacagctatt cgaagaaacg gtagcaaaag acattgcttt cggtcctaaa
    15781 aactttggtg tcagcgaaga agaagcacta gtcctagcaa aagaaacatt agaacaagtt
    15841 gggctggatg aaagctattt ggaacgttcg ccatttgaac tttcaggagg gcaaatgcgt
    15901 agagtagcaa tcgctggtgt gcttgccatg aggccggaag tacttgtatt ggatgaacca
    15961 acggcaggac ttgatcccca gggcagaaaa gaaatgatgg agatgttttg gcgtcttcat
    16021 aaggaacagc agatcactat tgtattagtt acgcatttaa tggatgatgt agccaatttt
    16081 gcagattatg tctatgtgtt ggaaaaggga cgaatcgtta atagcgggga gccgcaagag
    16141 gtctttcaaa acattgaatg gctaaaagaa aaacaactag gcgtacctac tgccaccgag
    16201 tttgcagaag aattaatggc taaagggatg aactttgcta ccttgccttt gacagctgaa
    16261 gaattagcag acgctatcgt ccattatgct ggaggaaatg cccatgatga ataaattgat
    16321 atttggtcgt tatatgcctg gagattcctt gatccatcga ttggatcccc gagctaaatt
    16381 attagccagt ttttatttta tcggaatcat ttttttagca aataattggg aaacgtacct
    16441 tttgttggca tttttcacat tactttctgt cttcttgtca aaagtagatt tgaaattttt
    16501 tatccgaggc attcgcccat tgatctggct aatcctcttc actgtagctt tgcaggtctt
    16561 gtttacaagt ggcggagaaa cttattggag ttggggtgtt ttccatatca ctgaatttgg
    16621 tgtgatgaac ggcctcttta ttttctgtcg attcgtgttg atcattttta tgtcgactct
    16681 actcacgctt actacaccgc ctttggaatt atcggatgcg attgagtact tgcttcgtcc
    16741 attgaaagcc gtacggttcc cagtccacga aatttcattg atgctgtcga ttgctcttcg
    16801 ttttgtgcct acattgatgg acgaaacaga aaagatcatg aacgctcagc gcgctcgcgg
    16861 tgttgatttt ggtgaaggaa acctagttca gaaaatgaaa gcagtcattc ctttgctgat
    16921 tcctttattt gtcagcagct tcaaccgtgc ggaagatctt gcgactgcta tggaagcaag
    16981 aggctacaaa ggtggggaag gaagaacgaa atatcgtgta ttgcattggc ataatagaga
    17041 cacgatggtt atcattgctt ttgtattatt aactgtgcta ctgatctttt taagaggata
    17101 aacgttcagt gggccgctat ctattctttt cttgctgatc tgtttgcaag aaaggatgca
    17161 agcagtgtat gacaagaaat attagggtct gtggcagact aaggtcacag gcctctttct
    17221 tttatctgaa aagaaagaac gaaaaaattt tatagaaaaa tcattattcc agataacctt
    17281 ttaggaacaa aggagagaaa aacatggtac gttacaaagc aatcattgcg tacgacggaa
    17341 caaattttaa tggattccaa aaacagccaa atgggcgtac tgttcaagaa gaagtagaaa
    17401 aaacattaca aaaaatggca aatggaaaag aaatcacggt ctttggttcg ggaaggacag
    17461 atgccggagt acatgcgatc gggcaagtaa tccattttga ttatccagag gagcgtccgt
    17521 tagaacgaat gcgtttcgcg ctagataccc agtcaccaga ggatattgct gtaagacaag
    17581 tagaaattgt ttcagaagac ttccacgcaa gatatttggt caaagaaaag acgtatcaat
    17641 ttcgagtgga tatagggaaa ccacgcagtc cttttcgacg ccattatgca agttattttc
    17701 cttacccact tgatttggaa aaaattcagc gggcgcttcc tgatttatta gggactcacg
    17761 atttcacctc tttttgtgca tcaggaagct cgatagagga taaaatacgt acgatctacg
    17821 aggctaagat ggaggtaaat gaaacaaaag acgaattgtt gtttacattt aggggaaatg
    17881 gcttcttgta taagatgata cggatcttag taggaacgct tttgaaaatt ggcaatggcc
    17941 gattgcctga agatagtatc ccagatatta ttgcgaaaaa agatcgtaat gcagcaggac
    18001 ctacagcaca ccctgaagga ctttatttgt atgaagtgaa atatgaataa gaggttatga
    18061 tagaagtggt cagcttcaag aaataagaag gtatttacga aaattacttc tcaaaatttt
    18121 gcaaaatttc ggcttatttt cgaagaagct acttctggaa caccgtttaa gaaagaggtc
    18181 gtgagcctgt catttaactc tgagtcacaa agagcaattt caaaaaacag cttctcatct
    18241 ttgatgaata tcgtgacttg tggacgaggc gttgataagt gaatcatctt tttgttacct
    18301 attttttctg taaccaaaaa acgatggaac gttgttttga ttgcccttgg tgcggacagt
    18361 ctgaacaggt tccatcgttt ctttattttc tacgtcttct tctgtgtgtg tttcgtggtt
    18421 gttcttctat tgctggatca atcgtgccat aaaaggggcg attgcggtca aggtaccaga
    18481 cataaagaat aaacaatgca atacatagga caaaaaggat tgcacctatc gtaaatcctt
    18541 caggcaagta actgaaagtg acagtatgtt tacctgctgg aacttttatc atcaagaatg
    18601 cgtctttgaa attgctgacc ttgactggtt tcccatcaat tttagctttc catcctttgt
    18661 cataaggaat agttgtcacc aacatggaat cttcagcggt atttacagtc ccagaagctt
    18721 ttctgccagt ggcttccatt tcgacacctt tttctttgat ctgattcatt gcggcttgat
    18781 aggctacaac atctagacca acgactttag gctccatgaa acttatttct ttcgtcccat
    18841 aaaaacttgc agtaaatgtc actgttgtct cttgaggata atagccaaga tcatagtatt
    18901 gaccagaaat gttgatttgc gtttttcgcg atgtaccatt gacagtgatc gtcgcactag
    18961 aactttctaa ttgcgcaaaa ttggttggga acaaacttaa gtaagcttgt gtatttgcag
    19021 gcacatggac ttcccaagtg agctcttttg gctggttatc tgctgtttct gagtaagtga
    19081 cgccagtcgt tgtctttgtg atcgtcgtat tcaactgatt cttcaaagta ggtgtataga
    19141 atgtaaaata ctgttcattt agaccagaca atgtattgat caagtttgtc tgactggtca
    19201 aattatctgt cactggctgc tcaacatcat taacggacaa cggtgcagta aatgctagtg
    19261 gcaatgcatt gacattctga tacagtgtat agtcgccgct ttgaccagct tggcggaagc
    19321 catatttact gaacgtgctt tcgtttttag aaatgttata tttgattcct gtaaacccat
    19381 ccatcagcaa agtattattg ttataacgga tattcaaatt tgtccctctt gaacggaaac
    19441 ctaatttatc caggtaagag gaagaattac gatttcttat agaagaaaac aaactgatac
    19501 cactgtaacc atagttgatc gaatcgtttg cagatactgg atccagattt tccaagcggt
    19561 aaaacgagtc ggaatctttt tttgtttcat ttacaagagc tttcaagtca ggataaggat
    19621 cagtatataa actgcgggaa gcataattcc aatcatcaag aatgccatgg atcatactgt
    19681 tcgtattgac cagtgcttca gcagacatga tcaataataa caatatactc agatgattga
    19741 atgaaagttt tttatgctga taaaatccca cagcaaaagc ataaatccct aagaaaacca
    19801 ctgtcaatac aaaggaagtc atcgtcacat aggtgtagct tccttcaggt ttgatgcccc
    19861 atgccaccgc aaagagagca gctacgacca aaatgattcc taacaacagc ccaccatcgt
    19921 cttgttctat cttttcccat ccttttcctg caagcaggag cactaagaaa gagaacagat
    19981 aggcataacg gaataaaaac atattcggtg catgcatacc atgccagaac aaattcaatg
    20041 gaacaagata aaaacttgcg ataagtatgg cgaaaagact gccgtacaaa aatttatttt
    20101 tcaacggtac tttccgactg gcaaaataaa acagacagag gactaatggt agcagacctg
    20161 cgtaaacgaa cggaatcgat ccgtattttg tcgtgtcata aacaccaatc atatttttca
    20221 tgattagatc cagataagaa gtcgcttctg ttttcaacgt agtgatctga ctcagtgcct
    20281 cgccatttga ccgaagatcc agcactgctg gcaatacaat gatcattgaa gcgccgcctg
    20341 ccagtaaaga agtgatacca taaggaacaa tcgttttttt atattctgac cagtttgtca
    20401 atagacgggc gataaaatat aagaaagaaa atacgccaat cataaaaccc atataaaaac
    20461 tggataaaaa caagagtaaa taactgataa ataaaagttt cggccgttta agatccataa
    20521 cacgatgaat ccccaaaatc actaatggaa gataaacaaa tgcatccagc cacatgatga
    20581 tctccgaatg tgcagtgata aatgagatca atgcgtaact gacgctaagt gcaacatgcc
    20641 cccagcgagg aatcttgaac gtacgtgcaa gaaaccaaaa agccaatccg gcacagccga
    20701 ccttaagcaa agtcagaaca tacagtgcat ctggcatcat ctggtttgga aaaaagaaca
    20761 ccagtggcgt gaagatcccg cctagatagt aagaaattaa agataaataa ttcaatccta
    20821 gagaagcatt ccatgtataa aaaatacttt gctttccaag aagaacattt cggaagctgg
    20881 catgaaaatt agaaaactgt gaaaaggcat cacttgccaa aatacttctg ctgcttccag
    20941 gatagattcc gatacttaga taaacaagtg ctaaaatcaa aaacggaatc aggaaactag
    21001 ctgctgcgta gcgtccgttt tctctaaaga atgactttaa attttttttc atatttattt
    21061 tccaccaaat ttctatgaag ttgtattcag tttatcatat ccttgttttt ctctgcttaa
    21121 aaagagtcta gttttcacaa aaaaataaaa tttagaataa ataacttcat aatttgttcg
    21181 tataacaaaa ataaacgcaa aaaaagggt
//
LOCUS       DAIGOK010000042        21101 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.042, whole genome shotgun sequence.
ACCESSION   DAIGOK010000042 DAIGOK010000000
VERSION     DAIGOK010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.042"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="NOA01_001240"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="NOA01_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HCI0632447.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="NOA01_001241"
     CDS             complement(2020..2922)
                     /locus_tag="NOA01_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCI0632448.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="NOA01_001242"
     CDS             complement(2919..3611)
                     /locus_tag="NOA01_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HCI0632449.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="NOA01_001243"
     CDS             complement(3654..4295)
                     /locus_tag="NOA01_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCI0632450.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="NOA01_001244"
     CDS             complement(4333..4647)
                     /locus_tag="NOA01_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632451.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5987)
                     /locus_tag="NOA01_001245"
                     /pseudo
     CDS             complement(4635..5987)
                     /locus_tag="NOA01_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(5984..6916)
                     /locus_tag="NOA01_001246"
     CDS             complement(5984..6916)
                     /locus_tag="NOA01_001246"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HCI0632452.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="NOA01_001247"
     CDS             complement(6913..7689)
                     /locus_tag="NOA01_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HCI0632453.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="NOA01_001248"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="NOA01_001248"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCI0632454.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /gene="carA"
                     /locus_tag="NOA01_001249"
     CDS             complement(10933..12009)
                     /gene="carA"
                     /locus_tag="NOA01_001249"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCI0632455.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="NOA01_001250"
     CDS             complement(12014..13294)
                     /locus_tag="NOA01_001250"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCI0632456.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="NOA01_001251"
     CDS             complement(13313..14239)
                     /locus_tag="NOA01_001251"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HCI0632457.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="NOA01_001252"
     CDS             complement(14252..15535)
                     /locus_tag="NOA01_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCI0632458.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="NOA01_001253"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="NOA01_001253"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HCI0632459.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="NOA01_001254"
     CDS             complement(16323..17234)
                     /locus_tag="NOA01_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCI0632460.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /locus_tag="NOA01_001255"
     CDS             complement(17231..17695)
                     /locus_tag="NOA01_001255"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCI0632461.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="NOA01_001256"
     CDS             complement(17700..18398)
                     /locus_tag="NOA01_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCI0632462.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="NOA01_001257"
     CDS             complement(18385..18771)
                     /locus_tag="NOA01_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632463.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="NOA01_001258"
     CDS             complement(18918..20588)
                     /locus_tag="NOA01_001258"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HCI0632464.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="NOA01_001259"
     CDS             20771..21004
                     /locus_tag="NOA01_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HCI0632465.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
BASE COUNT         5902 a         4746 c         3743 g         6710 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaacg atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa a
//
LOCUS       DAIGOK010000043        20647 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.043, whole genome shotgun sequence.
ACCESSION   DAIGOK010000043 DAIGOK010000000
VERSION     DAIGOK010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20647
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.043"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(218..934)
                     /locus_tag="NOA01_001260"
     CDS             complement(218..934)
                     /locus_tag="NOA01_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCI0632466.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(935..2758)
                     /gene="glpO"
                     /locus_tag="NOA01_001261"
     CDS             complement(935..2758)
                     /gene="glpO"
                     /locus_tag="NOA01_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HCI0632467.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(2772..4268)
                     /gene="glpK"
                     /locus_tag="NOA01_001262"
     CDS             complement(2772..4268)
                     /gene="glpK"
                     /locus_tag="NOA01_001262"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCI0632468.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(4404..5864)
                     /locus_tag="NOA01_001263"
     CDS             complement(4404..5864)
                     /locus_tag="NOA01_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCI0632469.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(5854..7149)
                     /locus_tag="NOA01_001264"
     CDS             complement(5854..7149)
                     /locus_tag="NOA01_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCI0632470.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(7481..8158)
                     /locus_tag="NOA01_001265"
     CDS             complement(7481..8158)
                     /locus_tag="NOA01_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HCI0632471.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(8188..9279)
                     /locus_tag="NOA01_001266"
     CDS             complement(8188..9279)
                     /locus_tag="NOA01_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCI0632472.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(9380..9814)
                     /locus_tag="NOA01_001267"
     CDS             complement(9380..9814)
                     /locus_tag="NOA01_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCI0632473.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(10398..11855)
                     /gene="ascB"
                     /locus_tag="NOA01_001268"
     CDS             complement(10398..11855)
                     /gene="ascB"
                     /locus_tag="NOA01_001268"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCI0632474.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(11852..13747)
                     /locus_tag="NOA01_001269"
     CDS             complement(11852..13747)
                     /locus_tag="NOA01_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCI0632475.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(13805..15580)
                     /locus_tag="NOA01_001270"
     CDS             complement(13805..15580)
                     /locus_tag="NOA01_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCI0632476.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(15630..17126)
                     /locus_tag="NOA01_001271"
     CDS             complement(15630..17126)
                     /locus_tag="NOA01_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCI0632477.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(17320..18942)
                     /locus_tag="NOA01_001272"
     CDS             complement(17320..18942)
                     /locus_tag="NOA01_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCI0632478.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(19270..20583)
                     /locus_tag="NOA01_001273"
     CDS             complement(19270..20583)
                     /locus_tag="NOA01_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCI0632479.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCILMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
BASE COUNT         5950 a         4522 c         3391 g         6784 t
ORIGIN      
        1 catgtcctat ccttattaga atacaaagat attgttttcc tgacaggtac cgtttcataa
       61 gcacaccaaa taaactttgt ttattggcta ttcgacttac tgattagata ccagctacac
      121 tcacaaaaca taccccattc ttctaggtct tatttatttt ccacttaact atagcaagac
      181 acattttggt tgtcaaactt tagtatgttt tatagattta caccattaat gaatagacac
      241 ctaccgcgat aattgctccg atcattggac ctgcgattgg tacccatgaa tatgcccaat
      301 ctgaagttcc tttattagct attggcaata ccgcatgtgc taatcgagga ccaaagtccc
      361 gagcagggtt gattgcatat cctgttggcc ctcctaatga aagaccgatg gctaagatca
      421 atccacctac agcgaatacg ttcgttccag gtgcaaattc gttttgtcca aaagctagca
      481 acccaagaac taatacaaat gtaccaatta attctgtgat cacatttgct ggataattcc
      541 gtatagcagg acctgttgca aacgttccta aaatcgcgct ttcatctttc gttgcattcc
      601 aatgtggcaa ataagaaagc caaacaagaa tggcccctgc aaaagctcct aaaacttgcg
      661 caatgatgaa aggaacgaca agattccaac taattgcccc tgtcatcgcc atagctagag
      721 agactgcagg attcaaatga gctgggccca tgaatccaga aatatagaca gccattgtta
      781 cagcgagtcc ccaaccaaat gcgatgacaa tccagccaga agcctgtgct ttacttttgt
      841 ttaaattcac agccgcacac acaccatctc caagcaaaac taggatcagt gtcccaataa
      901 actcccccat gatttgagtc atatcactgt tcatttattt ctcccccttt agttcccgca
      961 gatctgattc tgcaatcaat gcttctaaac gtttagtctg ttgcgccttc tcctcttctg
     1021 tccattcaaa atatccagca attgcatcga tcacaggttg cttgatctca tcaagctgat
     1081 ctcgttcaaa caacagatga tttgtacgac gaatgagata atcgcctggg gccaaaacca
     1141 tttcttcttc taaaccataa cgtaaccgag ctgactcagc gagactcaaa ccaggataag
     1201 gtgccatttc ttttgctaat tcaaagatac gtcgggcatt agtcccgtaa aaatcagcga
     1261 tataagtagc atcttcttcc tctaaaccgg ctgctactcc ttcttttgct aattctgtca
     1321 ctgtttcttc taatttagtt ggatcgaagt ttccacctga aatctgatat tttttagagt
     1381 cgatttcttt cgtctctatt ccgtattctt cttttaataa ttggcgtatc aatcgcaaag
     1441 ctccttctgc catcttacgg taatcggtga ttttcccacc agataaagtc agcaaaccat
     1501 ctggttctct ttctaaagaa ctacctctgg aaatcgtaga aggtgctttt tcatcacggc
     1561 tgttttccaa atggttcaac acgtcttcta cttcagcacg agaaactttg ttttccttat
     1621 attcacttac tgtatcgaca actttattga agctcttgtc tgaaatcgat ccattatctc
     1681 caccattgta atcagaacca gaattaccaa tcaacagtgg acgaagtcct gcccagcttg
     1741 cttcgatatc attcaatgtg atatttgctt ctggatagcg atgattgatc acatccaata
     1801 gataatctac atcttcttgt gtgactttcg gatccgtaaa atctccttgg taatccgtat
     1861 ctgtcgtacc aaagtaagtc ttgttttctc ttggaatagc aaaaaccatc cgcccatctt
     1921 gctttcctgt atcgaagtat gtgggttgcg gtacaggcag ttttttcgca tctacaacta
     1981 aatggatccc tttagttgga cgcattttag gagagactgg acgcgtaaaa ttcaagtttc
     2041 gtactttatc tacccaagga ccactcgtat tgatcactaa ttttgctttg atttcgatca
     2101 cttcatctgt cagcaggtca cgagctttga cgccaacgat ttgatcgcca tcatataaaa
     2161 agccaaccgc tttcatttta cttactagat aagccccatc ttcggcagcc tttttgatat
     2221 tatcaatcac taaacgggca tcattgttgc ggaaatctag atagacaccc gcgcctttta
     2281 gcccttcttt tttcaaaaac ggttctcttt ccaatacttc ttctggtgtc agcgtataat
     2341 tctcatattt ggttcctgtc acatttgcca gtttgtcata aaggtccatt gctactttga
     2401 ccgagaacat attgaaggtt gttgctcctt cgccctcata gattggcaaa agcattggat
     2461 ctggttttgg gatgtgtggg gcaatacttt gtacgactgc acgttcacca actgtgtcag
     2521 ctactacttc cacatcaaat gtcttcagat aacgaatacc gccatgaaca agtttggtcg
     2581 agcgagagga agtcccttct gcaaaatcct gcatttcgat caatcctgtt ttgattcctg
     2641 atgctgctga ctggactgcc acaccggcac cagtgattcc gccaccgatg atcaacagat
     2701 ccaattcttc ttgctgcatt ttttgaatgc tatcttgtct tgtcttgttt gaaaacatat
     2761 ggatttcctc cttattcaaa tgcttgggta gcttttactg cttttttcca accgttgtaa
     2821 agtttttctc ggcgctcttc ctccatttga acttcaaata gcttgccagc ttcataaaat
     2881 tcgcggatct cgtctgtatc tttccaataa ccgacagcta atcctgctaa aaatgctgcc
     2941 cctagcgccg ttgtttccaa gttttctgct cgttggatcg gtacattcaa gatatctgtt
     3001 tggaattgca tcagatattc attgtttgct gcaccgccat ctactttcaa tacaggaatc
     3061 ttgattccag tgtcatcttg catcgtatcg atgatatctc tcacttgata agcaatcgac
     3121 tgtaaagtag ctttaatgat atcttctttt gtcgtaccac gtgtcaagcc aaacatagcc
     3181 cctcgtgctg cctgatccca ataaggagca cctagcccaa cgaatgcagg aacaacataa
     3241 acctcatctt cgcttgtaga gcgtttcgct gcgtcttctg aatcgctagc tttttgcaac
     3301 atttgaagtc catctctcag ccattgaatc gaagaaccag ccacaaaaat acttccttct
     3361 aaagcatagt aaacctttcc atttatgccg taaccaattg tcgtcaataa attatttttg
     3421 gataattgcg gtttttcacc ggtattcatg acaataaatg aacctgtacc ataagtattc
     3481 ttgaccatgc ctggttcgaa tgccatttgt ccgaataatg cggcttgctg gtctcctgcc
     3541 atccctgaaa ttggtgcttc gcttccataa aaatgataac cttgcgtata accataaact
     3601 tctgagttag aagtgacttt tggtaacatc actcgaggaa tatttagtaa atctaaaatc
     3661 tcctgatccc aatctagctg atggatattg aacaacatcg tccgactggc attcgagtaa
     3721 tcagtaacat gtgcttgtcc atcggtcagt ttccatacta accaagaatc gatcgttcca
     3781 aacatcaatt cgcctttttc agctcgttct tgcgcgcctt ctacatgatc aagaatccag
     3841 cgtatcttcg ttgctgagaa ataggcatcg atcactaacc ctgttttttc gtggaaaaat
     3901 tcttgatagc cttcattttt taagttatct gcaattccag ctgactgtct ggattgccaa
     3961 acaactgcat gatagattgg acgtcctgta tctttttccc atattactgt tgtttcacgc
     4021 tggttcgtta tgccaatccc agcaatctga tttggtttga tccctgattc gatgaacgct
     4081 ccggcaataa ctgactgtac agaattccag atttcatttg catcatgttc cacccaactt
     4141 tcttgcggga aatattgcgt gaactctttt tgagaactgc cgatgtgtct tcctttcttg
     4201 tcaaagataa ttgctcgtga gctagttgta ccttggtcaa tcgccatgat atacgttgat
     4261 tctgtcattg tgattccctc cattatatgc aaacgctttc tttaactact cttatcatac
     4321 tactgtttta aaaaaagaaa ctgtcaaaat tttattcttt ttttcttttt ttgacagttt
     4381 tttattatga agctattttt taatcaattc ttttcttttt tcagcgtctc tattcttctt
     4441 ttcaatcgaa taatgtcgta aggtgacgca aattctgaca gataatattc acactgtttt
     4501 ttgtttctct ggcaaaatgt gatcaccaaa tcatattctt taccttcttc ataacttcca
     4561 atctcaatac cgccgattcc cctcagttct ctgatcagat attgttgaaa agcaatacga
     4621 taaatcggca gactttctaa atcgattcca atcactactg gttttgcaat ataaaaatcg
     4681 atcatcaaaa gaacattggc atactggatc atcaggtaac taagctcact atcttcagct
     4741 cttttcctcg gcaattgctg caccgctgtt tcctgtaatt tcgccagtaa ctgtgttaag
     4801 gaatgcccga gcattttctg ctgctgttcc aaaagccgat cacgctcata gacttcgatc
     4861 tttccaacaa agaaaaacca ttcattgttg atctgggaaa tttggtagcc tactgccttt
     4921 tcttctttga ttttcaaacg acgaggacga taatgccata acatcgtttc ccgaatgtaa
     4981 gtatctaaaa cagcggttgg taactttttg ctccgtgtca aatcatactg ataaaatatt
     5041 tcttcgtcta ctatcgaaaa agaaacaaaa aagctgtaaa acatcattgt ttcgtataca
     5101 ttcagttctg ctgctgttcg actaagatgc atcacgatga ttggatcgat tgcttgataa
     5161 agccggtccg actgatagag taatttcttt tctttcaaag ttgcgtacgt acttttttca
     5221 ttcaatagtc tttttttcgt aatcccgagc cagcaagcaa tctgtgcttc tgctgatgcc
     5281 gtaaacgttg tttctaatac acggtccaat cctagaatga acggtctttt ttctggtgta
     5341 ttttgcaaaa aaagcggtcg ctgatcttcc gggatatatt ggaatagttc ataataaaaa
     5401 tatcgtatct gtagctcttc tccgtataat tgaccgtttt tgatttgtaa tccaaattcc
     5461 tctaagagct ggttcaattc tttgatcttt cgaaaaacag aagactcact gatattaaac
     5521 gctgtcgcta atcgaacgat ggatacttgc tgatgttcta ataaatagac gagcatctta
     5581 aacttcaagc tttcctgcaa taaaaacaat agaatttttt ccaaacttac atcttcgtcc
     5641 aacacaagca gcgcctgata ctcatttcgc tgtacttcta cttttttttc catcattcgg
     5701 ccaatcaatt caatatcttc caaatattga tcaaacgagg cttttgacag ttggacatgc
     5761 tctctcaaac cattgataga ggctgttccc ccttgcagaa caaggtttcg caaaatcagg
     5821 atctgtaact gctcttttct ttctaataat gcttcaatct tcataggcag tattcgccac
     5881 ctttgaccac accatatctg gcacacggta ttctggctgg aaaaatagct catgttcttc
     5941 taactgttca aacgtccatc cttcttctac tgcaattgtc agttggtcaa gaaggtggaa
     6001 catctctttt ttgctttgga gctgtgctcc aagaatcttt ttttcagaat caaatagaaa
     6061 tttgagttta actttgcttt gatttattgc tgtaagcttg tagtctacgt atcctgtttc
     6121 aattttttgg ggataaaaaa tgctctcttc ttctgtcaaa cctacgcttc ctaagaaata
     6181 gccaaaccac tgattgccga ctggacgata ggttccctga tcctttgtct gtgagccact
     6241 aattgtcttg ctgacatttc gtgctgtacg gacagcgtta tttactagag aaacatacat
     6301 tttttcattt gttggacgaa aagttacttg gatcgcatcg ccaatcgcat aaacgtcttt
     6361 ttgagacgtt tgcaagtagt catctaccca gatcgttcca tcatcattca atgttaattg
     6421 ttcttcccac atctgattgt cgggacgaga atgattagcc agcaaaacaa gatcatagtt
     6481 ctcctttctt ttttccgtct ctacaatagc ggtttcattt tcggtcaaag acacaacttg
     6541 ttcttctaaa aaaagtgaaa gcccttgatt tttcatttcc tttactaacg gttctgtcat
     6601 ttcaggatct agataacgaa aaagaattga ctttcggctt tcgtaaagat ggatttgttt
     6661 ttttcttgat gccaaccctt cagctatttc gattcctgct tgtcctgcac caataattgc
     6721 aattttcttt gcattcatga tcttttgctg aagagccgtt acgttttgta tctttttaac
     6781 tgggtgaata agcgttgatt ctgcagaaat atttcgaaac ttttgatttg aaccagtcgc
     6841 tagaatcaaa cgatcaaaat ccaatttttt tcctcctgct aaaacgactt ttttttcttt
     6901 caatcctatt actgtctttt ctgtgtaaac atggattcga tagcggtcag tcagttcttc
     6961 tttcgtgatc caagaaagtt gtttttcact ggtaaccttt ccttttaata tcaaattgaa
     7021 cccgctggga atccatccta acttcgattg cttttcaatc aaatatattt cacttgttgg
     7081 catcaattgg cgaagataaa tagccgcatg aatccctgca aacgacccgc cgacaattac
     7141 aatgttcatc tgcattcctc tttcttataa ggtgttctac tgctaacttc tcaatcagaa
     7201 aacacagtat tcataactcg ctttcaacct tatgatacat agcgatgcag cagaatcaac
     7261 cctctccatc ataccgtgtt ctaggtctgc ttctcaataa tgtactagtg gacctaattg
     7321 aagagtatca tttctgaaga gatgtttcaa aaaatgtact cttataaaag aaaaaaactc
     7381 gccttttacg tacctctgtc caaataggaa acagaaaaga acgaaaagac gagttttagc
     7441 gtgtgtaatg taatagttag aatagacaca tcatttattt ttaattcttt aatgcttcaa
     7501 caactgcttc gcctaattga tgagcagatt gaggattttg ccctgtaaga agccgcccat
     7561 caatttgaac cacttctgaa aaagctgctc cagactgata gtttgctcct ttttctttca
     7621 aagcatcttc tgctaagaat ggtacttcat tcgttgtacc atttgcttcc tcttcttcat
     7681 tactgaaccc agtcactgta cgtccagcta aaatagattt tccagactca tctgtaactg
     7741 gcaacaatcc tacaacaccg tgacaaacag ctgagaccag cccttcattt cgatagattt
     7801 gtgaagcgac tttcccaaga tctattgctt ctggaaaatc ccacattgtt ccgtggccac
     7861 cagcataata aaccgctgca taatcatttg catccacatc atcaggacgt aaggtatttc
     7921 ccaaggcccg atttctgaaa tcttcatctt cataataggc ccaatcgatt tcatccatat
     7981 tcaaaacact ttgcggttct aaaggtacat atccgccttt aggactgaca aaatctgcat
     8041 cgatcccatt ttttactaat acatcataaa aatgagtcag ttcacttaac cacaaacctg
     8101 taggacgctc gatcttttcg tacttttcta catttgttaa tacgatcaat actttcattt
     8161 tcccatcctc tttctataaa aattgcgtta ttgataacgt gctttcaaga aattatataa
     8221 tgggcttggc aaataaagct ctttatatgc ttcttcaaaa ggtttcgtta caaattcatc
     8281 ctcacggttt tccaataatt tctctgccca gtcaggatcg acgatcattt gctgtccaat
     8341 tgctgcaagt tctgcatatt tcaatacctg ctctacatct tcgcgattgc ggatcccacc
     8401 gacaccaact aaagggatct ttccttgaat cgtgttatgg accacttcaa gaatcgattt
     8461 atcgttgtat ttatcagaac gagctattcg atcatacata tttaatgaaa catgcagata
     8521 atctagtttt gactcacgta atcgttcaag aagccagatc gtgtcttcaa aacggatacc
     8581 tggtgtttca tattcttctg gtgagaaacg ataccctacg atgaatggtt ttttagcata
     8641 ttgttcaacc gctgagaaaa ccgttgttaa taattcttca ataaaacgat aacgattttc
     8701 caagctaccg ccccattgat cctctcgtct attcgaatgc ggagagaaaa attgttggat
     8761 caaatacgta tttgctccat gcaactcgat tccatcaaat cctgattcaa tcgctctacg
     8821 tgtggctttg ccaaatgctt ggattgtttc aacgatttct tcttcactca ttgctcgtgg
     8881 tgtttccgca tcaggacgtt ttgctgcaac tgcacttgca gatacaggct gttctccaga
     8941 taaagttgct cgacttgtca ttcgtccacc gtggaaaatc tgaataatcg cttttgcgcc
     9001 ttctccttgg attgctttcg ctagctcact taatcgagga atcatttcat catgagcgat
     9061 gctgagttca cctggccagc cttttccgat atcttggaca tttgcagcac cagtaataac
     9121 agcaccaaga cctttagaac gttgtttata ataagtgatc tcatcttcgg tgatcactcc
     9181 attgaaaaag ctgagttgtg tcgtcatagg cgacagcatc aatcggttct tcaattgaac
     9241 tcctgaaggt aaagtaattg attgatcaaa ttgtgacatt gtcattcctc ccataattcg
     9301 ctctgattaa cctatcgaga atcatctatc attaaaccac tcgattagtt gtacatgcaa
     9361 ctaatttgct taaaaaattt tatttttttt gtaacttatc tgttgcatca ttcattgtct
     9421 gggctaattc tttacttgct tcatttccca aaactgcttt gtatgactga tgaagttctt
     9481 cccagctacc atcgacttcc ttagcaaatt ggcgtccttg ttctgtaaga taaactaatg
     9541 acaagcgacc ttcgctaact gttttgacgt atcctttata ttctaatttt tcaacaaaac
     9601 gagcaatcgt tgatggggcg gaatagagca tttgtgcaat atctttttgt ggtattccat
     9661 ctttttcttt taatatcaat aaaataaaac cataagttgg tgccagacct gtccttctaa
     9721 acgcttcatc agctaattta ttcaacatgc gatctagctt tttcacgcta aagaaaagac
     9781 attcatctaa cgctttttca attggctttt ccatttcatc ccctccattg gcgtacatat
     9841 catagtatga tttttatttt ttgtcaaaaa acacggaata atgtccgcct cctctgtcta
     9901 gatttgaaaa aaagaagtag ccatcttcaa agaatttcgg cttgttttcg acaaaactcc
     9961 ttctggaaca ccgtgaatca gaggttgtga aaaagtcgtt tagttttgag tatgaagaaa
    10021 aattttgtga aaatggcttt ccacattttt gcataaattt ggcttaatac cgaaaaactg
    10081 gcttttgaac accgtttata tcagaggttg tgaaaaagcc gtcctgcatc aagtaataag
    10141 aacagccaaa caaaaatagt tcctcatatt ttgtcgtttt ttgagctaat gccgatggtc
    10201 agctgattgt acaccgttta ttgagaggtc gtgagcctgc cgttgaactc cgagtcacaa
    10261 ggagcaattt caaaaaacag cttctcatgt tttatgacta tggtgacttg tgatcgagga
    10321 gttggcagga gaatactgtt tattcgtaaa gaaggggctg tgacaagact tttgtcacag
    10381 tcccttattt ccttttatta taggatttcg ccatttgatt ggatgacttg tctgtaccaa
    10441 tcaaatgatt ttttctttaa ccgtttcttt gtgccattcc cttcgtcatc ttgatcaaca
    10501 tagatatatc cataacgttt tgacatttgt gaagtagaag cgctgacgat atcaatgcag
    10561 ccccacgacg tataaccaaa taaatctact ccatctttga tagcttcctt catctgctca
    10621 atatgctgct tcaaatagtc aatgcggtaa tcgtcttgaa cggttccatc ttcttctact
    10681 ttgtctatag ctccgagtcc gttttcagct atgaacagtg gtttttgata cctatcatat
    10741 aattgattca aagcaattct caaaccctta ggatcaatct gccagcccca ttcacttgat
    10801 ggaagatacg gatttttaat tcctccagtt attaagtttc cttggacccc ttcccaattt
    10861 tctggatgag cactgactgc actagacatg tagtagctaa aagaaacaaa atcaacaggg
    10921 taggctgcta agatttcttc atcacctact tctattttta agttgacacc tttttcttca
    10981 aacattcgtt gcgtatacgc tggataatat cctcttgctt gaacatcact aaagaacaaa
    11041 gaagttcttt gctgctcaat tgttttttga atatcatccg gatcacaaga atacggaaca
    11101 agaagctgat agctgatcat acaaccgact tgtgaacctt tgataatttc atgacaagct
    11161 tttgtagcta aagcacttgc tataaactga tggtgtgcac cttggtattt cgcttgttcc
    11221 aaatggggat ggttttcttc aattactcct acgctaacaa aagggtgatg ttttacacag
    11281 ttgatctcat tgaaagtcat ccaatatttt actttatttt tatagcgttt gaatactgtt
    11341 tcagcgaaac gaacgtaaaa ctgaataagc tttcgattag gccatccacc atattctgta
    11401 accaaatata acggcatttc ataatgggaa agagaaacga ctggctcaat cccatatttt
    11461 agacattcgt caaacacttg atcataaaaa aataatcccg cttcattggg tgtttcttca
    11521 tcaccatgag gaaaaattct tgtccatgcg atagacatcc gatagcattt aaaacccatt
    11581 tcagcaaata aacggatgtc ttctttaaag tgatgataga aatcatttcc gtgtctttta
    11641 ggataataat acttgctgtt gggatcctgc gccgctttta cttgctgatg cgtcatcata
    11701 tgccattgat ctgtccattt ttctttaggt aaatcgctgt cgaatgtata cacatcagac
    11761 acagataatc ctttttcatc tacatcccat gcaccttcac attgattggc cgctgtcgct
    11821 ccgccccata aaaatccttc tggaaatttt ttcatatcga tccccctaaa cttgctagtt
    11881 ttcttgtttg attatttttc ttatgactag taatgatatt tttgaatcta ctactatttg
    11941 taacaataac cattgtggtc ggatcatatc cctctttgat taattcttgc aaatctactt
    12001 ccatcaataa atcgccttta cgtacttttt ttccatcaga tactgcactt tgaaagtatt
    12061 tgccatttaa attcactgta tcaataccga tatggatcag tatctctgct ccttcttcac
    12121 tgactacccc aattgcatgt ttcgttggaa aaactgttga aacttccccg tttacagaac
    12181 tgtagatttt tccgtccgtt ggaatgatcg caaacccttc tcctaaagat ccactagcaa
    12241 atgttggatc gtttacttca gataaatcaa ttatttcacc tgctgcagga gtacttaaca
    12301 ctatatcctt tgaggattct tcttcgattt catccttgta gccgaaaaga aaagttagca
    12361 tacaggaact gatcatcgct aagaaataac tagcaactag ccaaacaaat ccttcaccaa
    12421 tataaatcgg gaaagacaaa atacttcttg tagcaactgc caaagcaccg gctcctgcgg
    12481 caccagctac tccaccaaat atagctccac atatacaagc aatgtaaaat ggtcgtttat
    12541 atttaagagt cacaccataa acagcaggtt cagtgattcc aaataatgct gtgataaatg
    12601 cagataaaga taaacttttt actttcttat ttttcgtttt caaaaatacc ccaagtactg
    12661 ctcctgcttg cgcagctacg gcagtcatac atgctggacc tagcgtatca cgaccgtaga
    12721 aagaaagatt gttggtcatt actggtacaa gtccccaatg taacccaaaa atcactaaca
    12781 cttgccaaaa tccgccgata acagcaccag ataaaacagg gctaaagtta taaacagcta
    12841 aatatccagc tgcaatcagc tgacttgcat aatctgcaat tggacctaca actaataaaa
    12901 taattgggac catcacgatg atacaaatga atggtgtcaa taaatttcga atactttcgt
    12961 gcaagctttt gcgaatctta ggctcaatat aagaaagaac ccatacagct aaaattgctg
    13021 gaataaccgt acttgtatac cttgctagaa tcacgggcat ccctagaaat cgcaaggtta
    13081 tgctatcact atatgcactg acaatcgtag gataaacgag agctgctgca ataaccatag
    13141 cgatgaaacg atctgtattg aacttttttg cagcagtata tgccaaaaat ataggcaaga
    13201 aatagaaaaa tgcatcagct gctgcattca aaatgatata agttcctgat tcagtagtta
    13261 ataaattcaa actcgtacat aaggcgagta ctcctttgat caaaccactt cctgccaaga
    13321 cgcctaaagt cggggcaaaa acagcggcga tcatgtctat aaaagagttt aaaatgcctt
    13381 ttttctcagt tcctctcttt ccactagatg catcatcttc tagtttcatt tgtttggata
    13441 atacgtcata cacttcattt actgcatttc ctactacgac ttgaaacatt cctccagctt
    13501 cgactacagt gatcacacct tctagttgct ctaccttctc cttatctgcc ttcttattat
    13561 cttttagggt aaagcgtaac cttgttgcac aatggtaaac attgctgaca ttttcttcgc
    13621 ctccgacaaa tcggacaata tctttcgcta attgtgaata gtccattttt acttccccct
    13681 tgttaattaa tgccgtagca atccttctta caagataagt atagttctta gaaaacggtt
    13741 tctccattca attagtaccc cttctaatag cgaacgaaac tgtacaatag tgtgtatggt
    13801 ttacttagcc gagcaaatca ctcactgctt gaaagaaaac aagtatatcg tttctcttat
    13861 agtcaaagga gacaaaaaaa acacgcttga tcttcctttg ctgatgtata agtggtatct
    13921 caagctgata ctctgtgatt tttgtttcta cttggctttt gataagacag cacaacaaag
    13981 tctcggcatt taacggaagc ataactaggt cagaactagg agagattagt tgctgaatgg
    14041 aatctatctt tttatggatt tttatttgat tttcttcttt gtagtaatga tcaagcagtt
    14101 ttgttttctt tttttcttca actatggtca gcttttcacg caaaatctga taaatctccc
    14161 aagattcaaa ctcatctaga acactaggta gataaatcaa atcatcccac tcaaaaataa
    14221 gctcttgctc agtagtcaaa attgcatcat attccgttat tttgtttgat tgatttttga
    14281 aaagatacac tggttctatc cggcattctt tgatccacat gttcatactt tgttgaatct
    14341 gtttttttag tgtattcaaa atactaattg gctggttact aacaatcagt atctcccctt
    14401 gttttcgaaa aggattcaac gcttcggcaa gatacaaaac aaaataagcg ctttcagcta
    14461 aattgggacg taattgaaaa tgtattggga agaaacgccg aattaaataa atttctaaag
    14521 gataacggga gatgatcttt tgtacaaaat gattcatggt attatgtccg gcatccatcc
    14581 tcgttttcat ttgtttgata tgggttagca aaaattctgt ttcatggaac aataacgaaa
    14641 caggaagatc taaaaaacga ataatcgttt gctcaaatgc tcttagattc ttctctattt
    14701 ctttgttttt ttcatcatta atcatcaaat aatttaactc taatagtcga ttttcaattg
    14761 cttgtttttc ttgattggaa aaagaatagg gtagttctga tgataacttt tttgtgaggg
    14821 tctctaccat cggaataaat aatgtatacc tgtttgtttt ttctaaaagt tcttctcttc
    14881 gaagaatggt caaaacgaga taccgagaaa atcgtatgaa gtcctctcct gaaacaatat
    14941 aattatatga tataagaacc tcttttaatg tcttttggac agtcggcatg atattggaaa
    15001 aaattgtact gatttgttga ggaaaccttg cgtgttggat caccgtttct gttccaacaa
    15061 gagataagaa aattcttttt gaattttctt cagcaatgat ttttagtcct tttgtcgacg
    15121 aaacttccag ttctaattta ggattgcgct cgatccaacg caaaatcgtt tttatttgta
    15181 atgatacggc tgtttttgaa agaaagaaaa tgtctgctat cttttgcata ctgacatacc
    15241 ctttttgaaa caataagtac gtcacaatag ttgaagctcg gaaatccatg ctggaataga
    15301 ttttatgttt cgtcacctct tgtatgattt gctgtttagt ttcagattca attgattcta
    15361 aaaaataacc tgaacgttta tttgactgga taatgatttt cttctcgtag atagcaagca
    15421 tatttttgat ttcagtttgg atcgttttct tgttggtacc taaagcttca gctaattctt
    15481 cgcttgttat ccaatgattc ttatttctta atactacaag aatttggagt tgtcgtttat
    15541 ttaagtccat ttgcttacac ctcctctttg ttataaacat acaggaaaaa cctctcaaat
    15601 gacaatagtc acttgcagag gtttaaaaat catttgaaga aagatctata ttcaaggttt
    15661 ttaccattag actggataat ttgtttataa tattcaaaac tatctttttt caatctccta
    15721 aagcttccgc tcccatcatt ttgtctatcc acataaataa agccatatcg cttcctcatt
    15781 tcccctgttc ctgccgatac gatatcgatt ggtccccacc ataagtaacc aatgatttgg
    15841 caaccatctt ttattgcctc agcaatttcc tgaagatgta tttttagata ctcacaacgt
    15901 tttggatcgt ggattcgatt caattcatct ggtgcttcat ctagtccaat gccattttca
    15961 actatgaata atggtaattc ataacggtct gtcaattcat tcatgacata acgaaaccct
    16021 agaggatcaa ttggccaact ccaaggttcg ggagattttt ccttcaaaaa gggattatcg
    16081 aaaccatttg ctccgccagt atctacgcgc atttctgctt cttttttata gacagcgcta
    16141 cgataataac taaatgcaat ataatctgcc gtgttagctt gcaagcactg ttcgtctcca
    16201 tctaacatta ctggtgcaca gtcttcttcc ttccatgtcc tgtaaatata agctggataa
    16261 tgccctcggc acatgacatc ggagaaaaac catgaattgc gtttaaattg cagtgaacca
    16321 aatacattct ctggatcaca gtcatatgca taagtcgcta atgcagaaaa gctacacata
    16381 atacccatct tagcatttgg atcgattttc tttaataatt gagtagcacg ggcgttcgct
    16441 agaaacatat gatgacaggc ttgataaatc actttttttg ttagtgtagc aatgggttca
    16501 agtttatctc tagcagggaa tgtaaccaat ccagcagctg cataaggcat acgaaaagca
    16561 ttattgatct cattaaaggt catccaatat ttcactttcc cttggtagcg ttcaaagacg
    16621 gtctgcgcat aaaattcaaa aaagtcgatt aattttcgat tttgccaccc cccatactct
    16681 cctaccaatg ctaagggggt ttcataatga ctaagagtaa ttactggctc gatatcatat
    16741 cgatttaatg tattgattag tctgtcataa aatacgagac cagcctcatt tggcttctgt
    16801 tcgtctcctc ttggaaatat tcgtgcccat gagatgcttg ttcgaaaagc ttttaaacct
    16861 aactccttca gtaaacgaat atcctcttca aatcgatgat aaaaatcaat cgcttcatga
    16921 gaaagataat ggagtgattc gtctaaagca atctcccaaa tctttttttc ttcattccac
    16981 tgaatactag gatgctggtt catgatcccg ataagcgtat ctgtcacatt aggtaatttg
    17041 ccgtcttcca accatgcacc ttcacattga ttagcagcta ctgctccgcc ccataaaaaa
    17101 ttttctggaa atatttcctg atccataact atctctcctc gctatcaaca atttcttact
    17161 tgaagtatag ctcgattcat caaaacaaga tattctaact atttcggcag aagagaagaa
    17221 aaaaattgaa tgctactctt catttttaat atttatatat aacaaataca taagacgatc
    17281 cccgaaagaa aacactcgtt aaccatctaa gcaaacgatt tattttactt ccaacacaaa
    17341 tgcttcataa ggtcgtaaat caactttgcc gtctggtaac tgtttcagag gataatttga
    17401 cacaataacc cttaaagctt cacgtggaat agtgaacgtc tgcttttcac tggacatatt
    17461 acaaataacg agccattgct ggtcttcata tatgcgagaa taggcaaaga catgctcagg
    17521 cgtcttttcc actaattcat agtctcccca gacgatgatg tcatgttttc tacgcaaatc
    17581 gatcaatttc ttgtacatat aaaaaacaga atcttgatct gctaacgctt cttctgcatt
    17641 gatttcttta taatttggat tgactcgaag ccatggaatt ccagtagtaa acccagcatg
    17701 ttcacgggcg ttccattgca taggcgtcct agcattgtct cttcctttcg ccttgattga
    17761 ttccaagatt tcctcttttg taaatccaga agaaatcctt tcgttataca tattgattgt
    17821 ctcaatatct tttgcttccg cgatatcatc gattgggcaa ttcgtcatgc ctaactcttc
    17881 tccttgatag atataagggg tgcctttcat catatggagc aagatagcaa acatcttggc
    17941 actttctttt cgatacacat cgtcatttcc ccaacgagaa acaattcgcg gaagatcgtg
    18001 attattccaa aataaactat tccatccttc atcacctaaa gatgtttgcc atttggataa
    18061 tacttttttc agttcagaga tactcaaggg tttcaaatcc catttgtctt tgccttcttg
    18121 ctgatctaat cctatgtgtt cgaattggaa gaccatggat aattcatttc ttttaggatt
    18181 ggaataaagt ttagcgattt cggtagttgc tccccatgtt tcgccaacag tcatcagatt
    18241 tttgtcacca aaagttgctt gattcatttc ctgaagatac tcatgcagtt taggtccatt
    18301 acttgtgatt tctttatccg gttctttgcc aatcatatcg atcacgtcca tacgaaagcc
    18361 cccaatacct ttatcgatcc agaaattcat catttgataa atctcttggc gaacctgttt
    18421 atttttccaa ttcaaatcag gttgacgctt gctatacaag tgcagaaaat attgccccgt
    18481 tttttcatcc aactgccaag cagaaccact gaatattgag cgcaatctat taggctcttt
    18541 tccattgacc ggatcacgcc aaatataata gtcccgataa ggattttctt tatttttacg
    18601 ggcctcaatg aaccacggat gttcatcaga agtatgattc accaccagat ccatgatgat
    18661 acggatattt cttttctttg cttcttcaat cagttcttcc atttcttcca tcgtaccgaa
    18721 ttctgccatg attgcttgat aattactgat atcataccca ttgtcatcat tcggagactc
    18781 gtatactgga gaaagccaaa ttgcatcaat acctaaatac gccagataat ccaacctgga
    18841 aataatcccc cgcaaatctc caataccatc ttgatttgaa tcttgaaaac ttcttggata
    18901 gatttgataa acaactgaat ggtgccacca tttttgttcc atgattttcc tcctataaat
    18961 ggatgtttct attttttgct taatcattta ttttagtctc tctcactaaa aattgtaacg
    19021 ctttcaaata gctattgcac cttgttatcg gtaacagaat aaaacaaaaa aacagctgag
    19081 gaaattccct caactgtttt ttgaaaggat tgatttatgc agggaagaat gctgatttca
    19141 caacttcttt ataaacggtg ttcactcagc tgtcccttgg tattagctca aaaaacggca
    19201 aaatataaga aactattttt tcgttttttt ctctaatact caggccagaa cgctgatttc
    19261 acaacctctt tatttcacat tcaatatcca catgaatccc acaaatacaa agaacagacc
    19321 atacatgatt ggagatactt cttttcctct tttagctgct aacatggtaa ttggatagaa
    19381 gataaaacct aatgcaatcc catctgaaat actataagtc aaaggcatgc cgatcaagat
    19441 caagaatgaa gggatcgcaa tctctaagtt tccccattcg atttgtcgca aagattgtgc
    19501 catcaatact cctaccataa tcaatgctgg tgcagttact tgtgaagtaa caactgatag
    19561 taatggagaa aagaacaagc ctaacaagaa gaaaatccct gtagtaatag ctgtgatacc
    19621 tgaacgtcct ccaacggcga taccagctga tgattctaca aatgcaccaa caggagaagt
    19681 accaaataag gaacccgcaa gcatagctgt tgaatcagaa gctaaagctt tccctacacg
    19741 aggcatcttg ttgtctttca tgaaacctgc ttggtttgct aatccaacta aagttcctgc
    19801 tgtatcaaag aaagtcacaa gcaagaatgt cagcactacg acccacattt gaagagaatt
    19861 gatgtcccct acatgtttta atgcaactaa aaatgttggt ttcaaacttg gtgctgcggc
    19921 aacaatttca gaaggcattg gaatcaaacc tgtgaccaga cctaaaatcg ttgtagctgc
    19981 catcccgatg aagatgccac ctggtacacg ccgaacaagc aagatagcag ttacgactaa
    20041 tccaaaaatc gtcaaccaag tagaaccaac gttgagtgat cctaatccta ctaatgtaga
    20101 ttcattcgaa acgatgatcc caccttcgct cagaccaagg aaagcgataa atagaccgat
    20161 accgccagaa atcgcatatt tcaaatctgc tgggatcgca tcaatgatca tttcccgcag
    20221 cttgaagatt gtgatcaaaa taaaaatcaa agaagcaacg aatacgccgg ctaatgctgt
    20281 ttcccatgga atccccattc ctaaacaaac agaataagca aaaaaagcat tgatcccaag
    20341 agcaggtgcc gttccaattg gataacgggc taataccccc attaaaatac agccgatcgc
    20401 actggctaat gcagtcgcag taaacactgc tccttcatcc attccagaag cacctaatac
    20461 agttggattt acaaataaga tataagccat tgagataaat gttgtaaatc ctgccagtac
    20521 ctcacgtttt accgtggtgt tcaatttttc tatttcgaag taggaaagta ttttttcctt
    20581 catcttccat ccccctaaat ccgaacatta taaataaagt ccgttaaaat ttcgatttat
    20641 taataaa
//
LOCUS       DAIGOK010000044        20555 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.044, whole genome shotgun sequence.
ACCESSION   DAIGOK010000044 DAIGOK010000000
VERSION     DAIGOK010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.044"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            79..807
                     /locus_tag="NOA01_001274"
     CDS             79..807
                     /locus_tag="NOA01_001274"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCI0632480.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            807..1061
                     /gene="purS"
                     /locus_tag="NOA01_001275"
     CDS             807..1061
                     /gene="purS"
                     /locus_tag="NOA01_001275"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HCI0632481.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1063..1734
                     /gene="purQ"
                     /locus_tag="NOA01_001276"
     CDS             1063..1734
                     /gene="purQ"
                     /locus_tag="NOA01_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HCI0632482.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            1735..3957
                     /gene="purL"
                     /locus_tag="NOA01_001277"
     CDS             1735..3957
                     /gene="purL"
                     /locus_tag="NOA01_001277"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HCI0632483.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            3942..5381
                     /locus_tag="NOA01_001278"
     CDS             3942..5381
                     /locus_tag="NOA01_001278"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCI0632484.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            5404..6456
                     /locus_tag="NOA01_001279"
     CDS             5404..6456
                     /locus_tag="NOA01_001279"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCI0632485.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            6453..7031
                     /gene="purN"
                     /locus_tag="NOA01_001280"
     CDS             6453..7031
                     /gene="purN"
                     /locus_tag="NOA01_001280"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCI0632486.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7028..8563
                     /gene="purH"
                     /locus_tag="NOA01_001281"
     CDS             7028..8563
                     /gene="purH"
                     /locus_tag="NOA01_001281"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCI0632487.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            8579..9853
                     /gene="purD"
                     /locus_tag="NOA01_001282"
     CDS             8579..9853
                     /gene="purD"
                     /locus_tag="NOA01_001282"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCI0632488.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            9975..10889
                     /locus_tag="NOA01_001283"
     CDS             9975..10889
                     /locus_tag="NOA01_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCI0632489.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11022..12587)
                     /locus_tag="NOA01_001284"
     CDS             complement(11022..12587)
                     /locus_tag="NOA01_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCI0632490.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(12580..14031)
                     /locus_tag="NOA01_001285"
     CDS             complement(12580..14031)
                     /locus_tag="NOA01_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCI0632491.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14045..15061)
                     /locus_tag="NOA01_001286"
     CDS             complement(14045..15061)
                     /locus_tag="NOA01_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCI0632492.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15281..16369
                     /locus_tag="NOA01_001287"
     CDS             15281..16369
                     /locus_tag="NOA01_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HCI0632493.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            16423..17949
                     /locus_tag="NOA01_001288"
     CDS             16423..17949
                     /locus_tag="NOA01_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCI0632494.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            17951..19156
                     /locus_tag="NOA01_001289"
     CDS             17951..19156
                     /locus_tag="NOA01_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCI0632495.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            19413..20390
                     /gene="guaC"
                     /locus_tag="NOA01_001290"
     CDS             19413..20390
                     /gene="guaC"
                     /locus_tag="NOA01_001290"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCI0632496.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
BASE COUNT         6594 a         3480 c         4704 g         5777 t
ORIGIN      
        1 attgctaaaa tttaaagtga aaaaacgaac aaaaaacgat gtatttgctt ttaatgtaaa
       61 taaaaaggag agacgtacat ggagaagaaa gaacttttgt atgaaggaaa agccaaaaaa
      121 atatatctta cttctgatga gaacttgatt tggatagaat atttagatca agcaactgcg
      181 ttgaatggcg cgaggaaaga ccagataagt ggaaaaggag aattgaataa tcaaatcaca
      241 agtgcaattt tccgttatct gactcaaaga ggaataaaaa atcattttat tgaacaattg
      301 tctaaaaccg aacaattagc aaaaaaagta caaattatcc ctctggaagt ggttatccga
      361 aatattgcag caggaagttt ctcaaaacgt ctaggtgttc ccgaaggtaa agaattcatc
      421 aaaccaatca ttgaattcta ctataaggat gaccagttgg atgatccatt tatcaatgaa
      481 gagcatatct atttcttaca acttgcaaag ccggaagaaa tcagtgagct gaaagcccaa
      541 gcgttggcta ttaatgaaat tttgaccgaa ttatttgcta aggtcggttt ggttttagta
      601 gatttcaagc tagaatttgg ccggtcgaat ggacaaattt tgcttgcgga tgaagtaaca
      661 ccggatactt gccgattatg ggatgaacgt acgaatgaac atatggacaa agacgtttac
      721 cgcagagatc taggtgattt gatcccagtc tatcaagaag tatataaccg tctatcggaa
      781 ctaactgaag aaaaggagcg agtatgatgt atttcgtaaa agtatatgta acctataaag
      841 attcagtgtt agatcctcaa ggggaggcag taaaaaaagc agtccatcgt atgggatatg
      901 aaacaatcga agatatccga ataggaaaat actttgagat acaacttgct gatgcagaag
      961 aaccaatcga tacagtgatt gaaacgattt gtgacaagct gcttgcaaat gtaaatatgg
     1021 aaacataccg atatgaaatc aaaaaaatgg aggaagtata acatgaaatt tgcggtcatc
     1081 gtttttccag gttcaaattg tgacgcagat atgttatggg cagttcgcga gatcatgggc
     1141 gctgatgccg aatttgtacg gcatgatgag tcctctttag aaggatttga tggggtttta
     1201 ctgccgggag gattctccta tggtgactat ttgagatgcg gtgcgattgc tcggttttct
     1261 acaatcatgg aagaagtgat tcgttttgct gaagaaggga aaccagtatt cggtacatgc
     1321 aatggattcc agatattgac agaagcagga cttttgccag gtgtactgag acgaaatgag
     1381 tctttgcatt ttatttgcaa aactgtacca ttgaaagtca accccaagac ggcctttact
     1441 tcagagtatg aagccgacga aataatctat cttccagttg ctcatgggga aggaaattat
     1501 ttctgtgacg aagaaacact tcaagaacta aaaaaaaata atcagattgc ctttacttat
     1561 aatgacaaca tcaacggcag cgtggaaaac attgcaggga ttacgaacaa agcagggaat
     1621 gtgttaggaa tgatgcctca tccagaacgt gcagtagaag acttgctagg atcagaagat
     1681 gggaaacggt tctttgcttc cattttaaaa aacttcggaa aggtgaccgt atagatgaaa
     1741 acattgaaag aaccgacagc tgaagagatc aagaaaaaaa gaatctatgc cgaatgggga
     1801 ttgactgatg aagaatatcg aatgatcgaa gaagatatct taggaagaat gcctaattat
     1861 acggaaactg gactgttttc tgttatgtgg agcgaacatt gttcctataa aaattctaaa
     1921 ccggttctac gcaaatttcc tactagtgga cctcatgtgt tgcaaggccc tggagaaggc
     1981 gcaggcatcg tcgacatcgg tgatggacta gcagttgtct tcaaagcaga aagtcacaat
     2041 catccttcag ccgtggagcc ttatgaagga gctgcgacag gagttggcgg aatcatcaga
     2101 gatattttca gcatgggggc tcgtccaatt gcgttgctag attcattgag gttcggcgaa
     2161 ttagataatc caagaacgaa atatttacta gaagaagtag ttgcaggtat cggcggatac
     2221 ggcaattgta tcggcatacc gactgtaggc ggcgaaattg catttgatcc ttgttatgaa
     2281 ggaaacccat tagtcaatgc aatgtgcgta ggtttgattg aacacaaaca tatccagaaa
     2341 gggcaagccg ctggcgtcgg taattcgatc atgtatgtag gtgcaaaaac aggtcgcgac
     2401 ggtatccacg gagcaacttt tgcctcagaa gaatttaatc aagaagaaga acagcaacgt
     2461 tctgctgtac aagtaggcga tccttttatg gaaaagctgc tgcttgaagc gtgtttggaa
     2521 ttaatcttgc atcattcgga ttctctaata ggtatacagg atatgggagc agcaggactt
     2581 gtttcatcta gtgcggaaat ggcttcaaaa gctggctcag gacttatatt gacattagat
     2641 gaagtcccac aaagagaaac tgggatgaca ccttatgaaa tgatgttatc ggaatcacaa
     2701 gaacggatgt tgatttgtgt taaatcagga gaggaagaaa aaatccaaga actatttcag
     2761 aagtatgatc tagatgcagt cactatcggt aaagtaacag acgacgggca atatcggcta
     2821 tatcatcaag gagaagaagt agcaaatctg ccagtcgatg cattagctga agatgcacca
     2881 gtttatcata aagaaatgaa agaaccagct cgaatcgtag aatttcagca gttagctcct
     2941 tatcagccag ttatcgaaga acctggacaa gtattgcttg atctgctgca gcaaccgacg
     3001 attgcttcaa aacgttcaat ctacgaaact tatgattcac aagtacaaac caatacagtt
     3061 gtccgtccag gaagcgatgc tgctgtgatg agggttagag ggacgaataa agcgttagct
     3121 atgaccacag actgcaacgc gcgctatctg tatttggatc caaaaatcgg aggacaaata
     3181 gcagtggcag aagctgctag aaatattatt gctagcggtg gtaagccctt ggcgatcacc
     3241 gactgcctga actatggttc tcctgataaa ccagaagttt tttgggaatt atcgacttca
     3301 gctgacggaa ttgctgctgc gtgtgaaacg ttaggaactc ctgttatttc aggaaatgtc
     3361 tctttatata atgaaacgga tggtcaagca atctatccaa caccaatgat tggtatggtc
     3421 ggattgattg aggatcataa acatatcact actcaggaat tcaaaaaatc aggggatcta
     3481 atctatatat taggtaagac gtttgcagat tttgatggca gtgaattaca aaagatgcag
     3541 cttggaagaa tcgaaggtgt gatccgaaac tttgatctat ccattgaaaa gagaaaccaa
     3601 gagttagtat tgacagccat ccaaaacgga ttgattgaaa gtgctcatga ctgttcagaa
     3661 ggtggcttgg caattgcact ggcagaatca gctttcaaac atcagctagg gatatctgta
     3721 cagtttgaat tgtcctctgc tcaattattt gcagaaactc aatcaagatt tgtgttgaca
     3781 gtcgcaccgg aaaataagac acgattcgaa gaaatgatgg gagatgctgc tgttttagca
     3841 ggtaaggtga cggatgaagc gataatcgaa atttcagcaa ctgacggtca gatcaaaata
     3901 gaaactgcag tagcaagaaa gtgctgggag gatgcaatcg tatgtcttat gaagtgaaga
     3961 gtttaaatga agaatgcggg atcttcggtg tttggggtca tccagacgcc gctagtgtgg
     4021 catacttcgg gctccatagt cttcagcatc gaggacaaga aggtgcaggt atcgtgtcca
     4081 atgatcgagg aaagcttaag gggcatcgag atttggggct gctttctgag gtattcaaag
     4141 atcaaagaaa attgcagcag ttaacaggag aagctgcaat cggacatgta cgttatgcaa
     4201 cagcaggcaa tggaagtgtg gacaatattc agccttttct attcaaattt catgatcagt
     4261 cgatcggctt agcgcataat ggtaacttga tcaatgctcg ttcccttaga aaagagttag
     4321 aggaaaatgg ggcgatcttc cattctaact ccgatacgga gatattgatg catttgatcc
     4381 gacgaagtga agaaacagat tttttaaata aattaaaaag tgctttgaat gaagtcaaag
     4441 gtggctttgc ctatttatta atgacagaaa cggcaatgat tgccgctttg gacccaaatg
     4501 gttttcgtcc attagcaatc ggacaaatga aaaacggagc atacgtgatt gcctcggaaa
     4561 cttgtgcatt agaagttatt ggggcacgtt ttataagaga tgtccagcct ggtgagatag
     4621 tgatcatcaa tgacgaagga atccagattg attgctttac gaaggacacg cagctttcta
     4681 tttgttcgat ggaatatatc tattttgcca gaccagattc aaacatcgca gggatcaatg
     4741 tgcatactgc acggaaaaat atgggacgga atttagcgaa agaatcgcca gtaaaagcag
     4801 atatggtcgt tggcgtacct aattcttctt tgtcagccgc aagcggttat gcggaagcta
     4861 gtggaatccc ttatgaaatc ggattagtaa aaaatcaata cattgcccga acatttatcc
     4921 agccaacgcc tgagctaaga gagcaaggtg ttcgaatgaa attatctgct gtacgaggag
     4981 tagtggaagg taaaaaggtg attatggtcg atgattcaat tgttcggggt acgacaagca
     5041 gacggatcgt ccatcttttg aaagaagcag gggcaaaaga agtccatgtg aggattgctt
     5101 ctcctccttt gaaatatcca tgtttttacg ggattgatat tcaaacgcgt aaagaactca
     5161 ttgcggcaaa tcacacgatt gatgaaatca aagaatgtat cggtgcagat tctttaggtt
     5221 ttttaagtga agagggtctg atcgaatcga tcggtctgga aaaagatgct ccgtattcag
     5281 gattgtgtat ggcttatttc aacggagatt atccgacgcc gttatatgac tatgaagaaa
     5341 aatatcagga atcactaaaa gaaaaagtat cgttctttta aaagcagacc aagaagggag
     5401 tatatgaatt caatggcaaa tgcatacgca aaagctggcg tagatgtcga agctggatat
     5461 gaagtagtag aacggatcaa gaagcatgtc aaaaaaacgg aacgtttagg tatgatgggc
     5521 gctataggag gattcggcgg ttgttttgat ttatcggtct ttcaattaaa agagccagtc
     5581 cttgtctcgg ggaccgatgg cgtggggacg aaattagcag tggcaatcga agcagacaaa
     5641 cacgatacaa tcgggatcga ctgcgtagcg atgtgtgtca atgatatctt agcacaaggc
     5701 gcagagccgt tatatttctt agactactta gcaacgggga aaaatgatcc gttacgtttg
     5761 gaaaatgtag taaaaggtgt agcagatgga tgtgttcaag gaaatatggc gctgattggc
     5821 ggagaaacag ctgaaatgcc tggtatgtac tcaggtaatg attatgatct agccggattt
     5881 gctgtaggga tagctgaaaa atctcaatta ttgacaggag aaacgatcaa cccaggagat
     5941 gtgttgcttg gaatagcatc tagcgggatt cattcaaatg ggtactctct tgttcgcaaa
     6001 attttctttg agcagcagca gttttcttta tcatcaaaac tgccagaatt gaaaaaaaca
     6061 cttggcgaag aattgcttga gccaacgaaa atctatgttt cttccttgct tccgctgatc
     6121 aaagaaaaga aaataaaagg agctgcacat atcactggtg gagggtttgt agaaaatatc
     6181 ccgcgaatgt taccagaatc tatggctgca caaatcaaac ttggctcatg gccgattctt
     6241 ccaatattcg gctgtttaca aaaatatggt gaaattcctg agatggagat gtatgaaatc
     6301 ttcaatatgg gcatcggtat ggtcctggca gtcgaagaat cgaaagtaaa agaaatagta
     6361 cacgaaattg agcaagctgg cgaaaaagtt tatgaaatcg ggaaagtgat cgccaaagaa
     6421 aaacatcgtg tggtgtttat aggagaagaa cgatgagaat cgctgttttt gcttctggaa
     6481 acggaagcaa ctttcaagcg ctagccgatt atctatcgaa aaaaggattg gaatcttcga
     6541 ttgactggtt gttttgtgat cagcctgaag catatgtttt gaaacgtgca actgcgttga
     6601 gtgtaccggc agattgtttt tcacctaaag aatttgattc aaaaaaagag tatgaagaag
     6661 caattttgca taaattgaaa gaaaaaaaga tcgatttgat cgtattagct ggatatatgc
     6721 gaatcattgg accggtattg ctgaaaaatt atgataaaag aatcatcaat atccatccgt
     6781 ctcttttacc tgccttccct ggtttgcatg gcatacgtga tgcctttgaa gctggagtaa
     6841 aagaaacggg tgtcacaatt cattatatcg accaaggtgt agatactgga ccaatcatcc
     6901 ggcaggaaaa agtgagaatt gaacaggagg acacgttcga ttctttagaa gaaaaaatcc
     6961 atagagtaga gcatcgaatc tatccagaag tgattagtga aattattgaa aatggaggct
     7021 atctgaaatg acaagagcat tgatcagtgt atcagataaa actgggattg tagcattcgc
     7081 ccaaggtttg attgaacaag gagtagaaat catttcaaca ggaggaacaa agcatgcgtt
     7141 ggataaagct gggatcaaaa cgctttcgat cgaagaagtg acagggtttc ctgaaatgat
     7201 ggatggccgt gtcaaaacac ttcatcctaa aatccatggt ggtcttttag gaagacgcga
     7261 tctccctagc catatggagg caatggaaac ggaaaatatc caaccaatcg actttgtttg
     7321 tgtcaattta tatcctttca aagaaacgat tatgaaacca gatgtcgaaa tagcagaagc
     7381 tattgaaaac attgatatcg gtggtcctag tatgctccgt agtgcagcaa aaaaccatga
     7441 atttgtcacc tctgttgttg atccatctga ctatgaagaa gtactttcag aattacgtca
     7501 gtatgggcat acttcacttt ctttcagaca aaaactagcg gcaaaagtct ttcgtcatac
     7561 tgcggcatat gatgcactga tcgctcaata cttaacggat tgggtggaag aaaaagaacc
     7621 ggaaaaattg acacttacgt atgaacgaaa acaaacgctt cgttacggtg aaaatagcca
     7681 tcaagccgct gccttttatc agtccgctct tctggaaaaa tattcgatcg cagcagcaaa
     7741 acaactccat gggaaagaac tttcttataa taatatccga gatgcagatg ctgcgctacg
     7801 gatcatgaag gaatatgacg agccaacggt agtcgccttg aaacacatga atccttgtgg
     7861 tattgggaca gcatctacga ttttagaagc gtgggaagca gcgtatgaag cagatcctgt
     7921 ttcaatcttt ggtgggatca tcgttttgaa tcgagaagta gatctgccaa cagcagaaca
     7981 gatgcataag ctgtttctag aaatcatcat tgccccttct tatacacaag aagctttgga
     8041 attactgcaa aaaaagaaaa acatccgatt actgacagta gactttgcgc aggaagaaaa
     8101 tcaaaatcga gaagaaacgg tctcagtctt aggtggactt cttgtacaag atcaagatct
     8161 ggcaatagaa aatgaagaag agtggaaagt cgtgacaaaa cgtaagccga ctccagcaga
     8221 agcaaaagca ttagcgtttg cttggagagc tgtcaaacat gtgaaatcta atgcgatcgt
     8281 actggcaaac gaacgtcaga cagtaggaat cggagccggg cagatgaatc gagtcggttc
     8341 agtaaaaatt gcgattgaac aagcgcaaga gaaaatggaa ggtgcagttt tagctagcga
     8401 tgcttatttt ccaatggatg attcggttga atatgcagcc aaacatggta taaaagcaat
     8461 cattcagcca ggaggaagca tcaaagacca agattctatt gatatggcga ataaatacga
     8521 tctagcaatg gtctttactg atgttcggca ttttagacat tagagaggag tcggataaat
     8581 gaacattttg gtgatcggtg caggtggtcg tgaacatgca ataagtaaaa aacttttagc
     8641 cagtccgaag gtggatacgg tgtattgtgc gccaggaaat ccggggatga caaaagatgg
     8701 tatcaattgt gtcgctatca gtgagcgaga tcattcagcg ttaatccatt ttgttaagaa
     8761 agaggctatt gattggagta tcgttggtcc tgaagttcct ttgttaaatg ggatcgtcga
     8821 tgattttatg gcggaaggat tgaaaatctt cggtccgaca aaagcagcag ctctgatcga
     8881 aggttcgaaa agttttgcta aacaattaat ggatacttat caaatcccga cagctaaatc
     8941 cagaagtttt gaatgttacg aaaaagcagc agcgtatatc aaagaaaatg gtgcgccaat
     9001 cgtagtgaaa gcggacggct tagcagctgg gaaaggtgta gtcgtggcta ccacagtgaa
     9061 ggaagcgctg gatgcggcag aacaaatgtt gaaccagcat cgatttgggg atagtagcaa
     9121 aaaaatcgtc gtagaagagt ttttggcagg agaagaattc tcccttttgg cgtttgtccg
     9181 taataccaag gtgtatccga tggtcatttc gcaagatcat aagcgggcat ttgataacga
     9241 taaagggcca aatacaggcg gaatgggtgc ttattcacca gtaccgcaaa tttcaaaaaa
     9301 gatgattgaa aaagctgtgg aagaagtttt aaaaccagcg gctttgggaa tgaaagaact
     9361 aggacgctct ttcacgggtg ttttatatgc gggtttgatt gctacgaagg aaggaccaaa
     9421 agtgatcgaa ttcaatgcgc gtttcggtga tcctgaaacg caagtcgttc tttctcggtt
     9481 gaagagtgac tttgcgcaag taatcgatga tttattagag aatcgtacag tagagttaaa
     9541 gtggcagcaa gaaggataca atgtaggcgt tgtggttgca gctgccggtt accccgaaga
     9601 atatgagaca ggaatggttc taccagattt tctggaagaa gagctttctg tttactatgc
     9661 tggagtttcc tcaaataacg gcaaactagt tggagcagga ggacgtcttt atttagtaga
     9721 agcatacggt gagacgatca aagaagcaca gaaaaaaatt tacaccgcgt taatggataa
     9781 aacgacagat ggaacatttt atcgtacaga tattggagaa aaagctgcca atagcgaact
     9841 gatcgtgaag taaatcagct gcttccattt ataaaaaata caacaaacat aatcggaagc
     9901 gccgatttta gtgaagcctt ttcataaaga gtgtggtaca cttttgttgt gtatataaat
     9961 aaggaggagt tcagatgaaa aaaattcaat taggtacttc tgatcagaaa gcagctgcag
    10021 tgatcttagg ctgtatgcgt atcaacaatg cggaagatcc agtaaaagtg attgagacag
    10081 cttatgaaaa cggcattgat ttttttgacc atgcagatat ttatggaaac ggtgaatgtg
    10141 aagaaatctt tgcagatgct ctagcaaaaa catcaatcaa acgagaagat ctttttattc
    10201 aaacaaaatg tggaatcgtt ccaggtgtga tgtttgattt ttctaaaaaa catatcattg
    10261 aatctgtaga aaaaagtttg acccgtctga aaatggatta tgtggatgct ttacttcttc
    10321 atcgtccaga tacattggta gaaccagagg aagtagctga agcgtttgat gaattggaac
    10381 ggtcaggaaa agttcgttat tttggcgtca gcaatcaaaa accaatgcaa atcgaattgc
    10441 tgaaaaaata tgttcgtcaa ccattagtag ctaatcagct gcaatttggc ttgaaacata
    10501 ctggaatgat cgatcaaggg attcatgtca atatgacaga tgaagcaagt atcgatcatg
    10561 acggtagtgt acttgattat tcccgattgc atgatatgac gatccaagca tggtcaccat
    10621 accagtatgg attttttgaa ggtgtattta tcgggaacga aaaattccct gaattaaacc
    10681 aaacattaga agaactagca gctaaatatc aaacgacacc aactggcttg gcttctgctt
    10741 ggattttacg ccatccagcc aatatgcaag tgatcgccgg tacgatgaac gtagggcgaa
    10801 tcaaggaaat cgctcaagct tcagaaatta atatgtctcg ggaagattgg tacgaattgt
    10861 accgtgctgc tggaaacatt ttaccttaaa gattgtcata agcaggtcaa aagggctata
    10921 aattaaaaag acagaataac ttgaaagctg ttttcagctt cgagatattc tgtctttttt
    10981 tattgcaaat ggaaagagta tcattgtacg gatttcgttt attagttatt tttgttttta
    11041 aggaaaagct gtgtccgata ttcttttgga gaaatccctt gttttttctt aaataaataa
    11101 ctgaaatagt tagggtcatt ataaccaatt tcaaaagcaa tatctgaaat acgatagtct
    11161 gattgctgca ataatttttt tgctaattcg attctttgtt ctgtcagata atcgatgaat
    11221 gtttggttaa acgattgaga aaaaatcgta ctgaaatgag agggacttaa tgcaacttgt
    11281 tgagcgaccc aagttaagga catatctgga tcggtaaaat tttctttgat aaaatccaat
    11341 gcatggttca aaattggctg atatttgttc cttgaaggct gtgagggcag agcagttaag
    11401 aaagaaatga acatataaac tgtcgaacgg taaagcgagt cgtcggcgct gattgctaag
    11461 cgttgactgg tggatgtgca atgaaccatt tcttgcggga ttttcttttc tttcaggcag
    11521 acatgctgga gtagcttagt caagacaaat aaacgatata atcgtgttcg agttggtgtg
    11581 ccttgtggtt tcgtcaagtg ttcgatgtac ttagatactt cgtttttcga taatgaagac
    11641 aaatcaagtg agaggtcaaa gtaaaagaca gaatcaactg gaagtttcgc ttcttgaatc
    11701 aaatcctcat agctgataat ttgttcttta agggaactgg cggtttcaag tatcatctgc
    11761 gtgatctcaa aagatgcagg taattcgctg attctttctg cgacaggacc aatggcgaca
    11821 gaaaaattat ttttttgtcc cttttctaat tcatggacca acgtttgggc tacctgataa
    11881 ctggtctcga tcacattttc ttcaagagaa tcaactatta aaaatttgat gaatcgagag
    11941 gaaacagcag agaagatcac ttgttgattc tccctaaata aaaacttcaa ataatcactg
    12001 agatgagcgt aatcttcaaa gtctgtactg acttggtttg ctgctaataa tacggtcatt
    12061 tttttccctg ctaaagagcg attgaaagat tcagcaagtt tcaatgtttc actgatcgta
    12121 agtgagcctt tgaacaaacc gttcaagaaa tgattttttt tcaattcgaa aacaagatca
    12181 gaagaagtgt gcgtttcttt ttgctgcaaa tctaaagcag ttactgcttt ttctattgct
    12241 tgtttcaatt cgctactttt aatcggttta agcagataat catctgcctg tacctggatt
    12301 gctcctttca tataatcgaa atcgtcgaat ccactaataa atatgagacg agtccaagga
    12361 agtagtttcc ttgcttcttt tgaaaaagtc aacccgtcca tgaaaggcat ttggatatca
    12421 gtcagaatga tgtcgggctg taagtcgata attgaagaca gtgccatctc accatctcca
    12481 gcttcgccga cataagtgat tggataataa gaaccaagga cagttatttg ttcccgcaag
    12541 ttattgcgaa tgaggtattc gtcttcgatg ataaaaactt tatacatgtt tgtttcctcc
    12601 aattgttttg ggagatagag ggatcatcag tgtgacagtt gttccttctc ggtatttgct
    12661 gtcaatcgtg ataggttttt gatcatttcc gtaataaaga aggagacgct tgtttacgtt
    12721 gtaaagtccg tagccgatac tgaaatctga atcgatccct ttggctaact ctttacgtat
    12781 ttctgccagt ttttctgcag ggatgccaat cccgttgtct ataactttga attggatcat
    12841 ctcatcaatt ttgcatgtgg taatacgaat aaatcctttg tgacggacgt acttcgttcc
    12901 atggtataca gcattttcaa tcaatggctg caagatcatt tttaaaatag aaacaggaaa
    12961 aagtgtttct ggtatatcga tcttatagtc aagcatatgg ccgtatctta tttttaagat
    13021 gactagataa tcattgatat ggctaatttc tttttcaaga ggaatccagt cttgtccttt
    13081 gcttaaagac gtacgaaaaa agccggaaag tgcatgggtc atagtcagag cttcttcaaa
    13141 gcagtcttgt tcgatcaaag aaataatggc atccaaactg ttataaataa aatggggagt
    13201 aatctgagct tgcaatacac gaacttcact ttgtgctaaa tggtattgtt tcaaagcgtt
    13261 ctcccgtaaa gactggttta gatcttccgc cattttattc atgctagcgg taagtgtttg
    13321 taattccagt gtttccggca gggttaaacg aaagccaagg ttccctgaag aaagttgttc
    13381 tgacatctcg atcagctgat tgatcggttc ttggaccttt ctctctaaaa agcgacgatt
    13441 ctttttcgca tagaaaaaaa tggtactaag aactccaagt tccactattg ttaatagaat
    13501 gagcgagcgg acaagtgtcg tatttttttt gcttgctaag tcgatctcca cacgaacaaa
    13561 atcttgtaag atatctgaca gcagttgagt gacagaatct acctgattga tgatctcttc
    13621 attggtatcc ataaaatctt ttgccaagat attattgatc agctcgtttt gataatcttc
    13681 cagtgtatcg atcgtacgta gcgatacatc taacgtacta atttctttaa gtgtatttgt
    13741 attatttttg atctctcgaa tatcattcct aagttcttct aggatatttt cctcattgaa
    13801 ctcatctacg gttacttggc cagaaatcaa gtcccacata ctttccaaaa ctttttgatc
    13861 aaccttcaaa gcaattgcat ctgctttttg gatatttgcg atcgtctgtt gataaatcaa
    13921 aagctgttgc gaatataaca gtatgctcag tagcaaaggg ataacagcaa atagaagcat
    13981 ggctttgttt gcttgggcaa aaatctgttt tatgcttaaa ttttttgtca tgatacttct
    14041 ccagtcaata atttctggtt gggatgttag ctatattctg ctgggaaaag acagtctcgg
    14101 aaataaaaat atctttaggt aatgtttttc ctgtttgaat atttccaaaa taacgagtaa
    14161 cggtattggc tacataccag ccgacatcag gtttacactc gactacacaa ttgacgattc
    14221 ctgagcgtag cttctcgatc atatcttctt gtccatcaat agttacaatg atcaaatctt
    14281 ctccaggttt gattgatgtt tcttttatcg cagctaaagc accgtgagtc atttcatcac
    14341 tatggctgta taatacatcg atttcggata aatcatttgt ttctaaatat cgtttcatct
    14401 ggtcttttcc tttcatccga ataaaatcac cgacaattcg gtgaacaata gtcatcggag
    14461 gtttatggtc gatggttcca tcttttttta gggttggttt acgattgatt gattccaaaa
    14521 atccttcgga gcgtagcttg gtcggtgtgg agttctctga accagcaagt tctaggattc
    14581 tgatttcctg tttctttgaa tttgtgaagt gattgctgac aaataaaccc gcccgatttc
    14641 cttctgcttt gaaactgggg ccaatgtggg tgacgaaaag ctcgggatca ttcgtttgaa
    14701 tatgtctgtc gacaagtatg acagggatcc ctgctcgctt tgcttctttt aatacagagt
    14761 cccacccgga ttcttgcaaa ggagtaaaaa tgatcatatc cactttatag gcaatgaacg
    14821 tccgtatatc ttggatttgc cgttcctgat tcatgtagcc atttcggtag agaacttggt
    14881 aatcttcttt ttcaagcgca gtgataattg attcagtatg ctttttccgc cagctactct
    14941 ccgttcctga ttgcgaaaaa ccaacgacta gtgggtcagg tgcttccttt gtattaggtg
    15001 tacaccccat catttggtaa gcgattacaa caagggctaa aaaaaataaa acacgtttca
    15061 ctttaaatca ctccatctgg atttgttagt actatcttat cagataaaag ggaaaatcaa
    15121 agtcaaaagg aatcaatcga aaaaaatcca agaaacttaa aaaaatccaa gacaaaaaaa
    15181 gtacacctaa aaaatttaaa gtaaatccca taaaaaaatc aatgttaacg ctttctttat
    15241 tagctatact cggtgtatca attgaaatgg gggataaaaa atgaaaaaca aatttagaaa
    15301 gattgttggg actatcgcta ttttgaccgc aagcattcta ttaagtgcgt gtggcaattc
    15361 aggatctgca gatgatagta cggggtatgt aggaatcgct atgccgacca aatcagcaga
    15421 aagatggatc gctgatggga acaacatggt ctcggaatta gaaaaactgg gttataaaac
    15481 agatcttcaa tatggagaag ataaagtaga aaatcaagta gcacaaatag aaaatatgat
    15541 tacaaaaggg gtagatacac ttgtgattgc ttctatcgat ggttcggctt taactgatgt
    15601 gttagctaaa gcaaaagaag cagatatcaa ggtgatcgcc tatgatcgtt tactgatgaa
    15661 ttcagaaaat gtcgattatt acgcgacttt tgataatttt ggagtaggtg tatcgcaagc
    15721 agcttatatt gaagagcact taggattgaa agaaggaaaa ggaccattca cgattgaatt
    15781 atttggtggc tcgcctgacg acaacaacgc actaatcaat tacaacggag tgatgtctgt
    15841 tttacaacca tatatggaca acggtcagtt ggtcgtgcca tctggacaga cgagtttcag
    15901 tcaaatcgct acgcttcgct gggatggttc tacggcgcaa gcaaggatgg ataatctgtt
    15961 aagcgcaaat tatactgacc aaacattgga tgctgttttg tcgccatatg atccaataag
    16021 tttaggaatc atttcgtcat taaaaggcgt ggggtatggg agtgaaagta aacctttacc
    16081 tgtgattact ggacaagatg cgacagttgc tggcgttaaa tcgatcattg ccggggaaca
    16141 aacacaaacg atctttaaag acacacgtat cttagcaaaa aatacgattg aaatgatcaa
    16201 agcaatcagt gatggagaag aggttccggt caatgatact gaaacttatg ataatggggt
    16261 gaaaactgtc cctacgtatt tagcaaatac agtatctgtc gataaagata attatcaagc
    16321 agagctgatc gacaccgatt attataaaga atccgatctg aaaaattaag aggaaccaaa
    16381 caaggaacta aagagatcta agtcagagag aggataagaa ttatggcgga ttgtatttta
    16441 aaaatggcag atatcaccaa aaaattttct ggaatccgag cgttagattg cgtgaatctg
    16501 atgattgaac gtggggaaat ccatgcattg tgcggagaaa acggagcggg aaaatcaaca
    16561 ttgatgaatg tcttgagcgg cttgtatcca tatggctcct atgacgggga aatcttttat
    16621 gatggaaagc tgtgtcaatt taaagatttg aaagatagtg aagaaaaagg catcgtcatc
    16681 attcatcaag aattagcact aagtccttat ctttcaataa aagagaatat ttttctcgga
    16741 aatgaacaag cagaaagagg cgtgattgat tgggaactga cagaaaagaa aacatatgaa
    16801 ctgctgaata aagtcggttt gtctatcgac ccgaatcttc tagtatccca gattggcgta
    16861 gggcaacagc aactagttga aatagctaaa gcattatcaa aatccgtccg tctgttgatt
    16921 ttggatgagc ctaccgctgc attgaatgaa gaagaaagcg cgaatttgct gagattgatc
    16981 cgagagttga aagagcaagg tgtaacctcc atcattattt cacataagct gaatgaaatc
    17041 gtggatatcg cagatcgaat caccatttta cgggatggac agacgattga gacactagaa
    17101 aatgaaatga tcagcgaaga gcatattatt cgcggaatgg tgggcagaga tttagttgat
    17161 cgttatcctg aaagaaaacc ggatattgga gaagtttatt ttaaagtaga agactggacg
    17221 gtctatcacc ctcttgatca cgagcgaatc ataaacgacc acatcaactt gcatttgaaa
    17281 aaaggagaga ttgtaggaat agctggactg atgggagcgg gcagaactga attcgctatg
    17341 agtctatttg gacattctta tggaagcaag atatccggac gtatattaaa ggaaggggca
    17401 gagatatcag ttaagaccgt tccttcagcc attgcaaacg gactcgctta tgtctcggag
    17461 gaccgaaaat ctttaggact gaatctgctg atggatattc gggaaaacgt gacgctgagc
    17521 agtttggata aaataagtca aaaaggagta ttagataaag aaaaagaagt aactgaggca
    17581 gaaaatttcc ggaaaaagat gcgtataaaa acaaactcca tccatcaaat tgtcagtagt
    17641 cttagtggtg ggaatcaaca aaaagtcgtt ttatccaagt ggctgatgac acaacccgat
    17701 attctttttt tggatgagcc aacccgtggg atagatgttg gggcaaaata tgagatctac
    17761 acgattatcg aagaaatggc agcaaatggc aaagctgtct gtcttatctc ttctgaactt
    17821 cctgaaatat tagggatgtg tgatcgaatc tatacgatga acgaaggacg tattacagga
    17881 gaagtctcgc gagaagatgc agatcaggaa gtcttgatga aattaatgac gaaagaagag
    17941 gtgggttaag ttggaacagg caaaaaatct tacggataaa aaagagaaaa aactaaattt
    18001 gaaaagtaga gcagctgatc tttttggtcg ctacagcatg gtgatcattt tagcgggact
    18061 acttattgcg tttcaattga tgacggatgg tattttctgg cgtccattga atataactaa
    18121 ccttgttctg caaaatagcc atatattagt actagctgca gggatgttat tagttgtgct
    18181 cttaggtcat gtggatttgt cggtagggtc agtgatggcg tttgttggtg caattgcggg
    18241 catgatgatg gtcaatcgcc aaatgaaccc gtggatgaca gttatcgcat gtcttgcagt
    18301 aggcgggctg atcggcgcat ggcaaggttt ttgggtagct tatgcaggga taccggcgtt
    18361 tatcgtgact ttagcaggat tgctgatgtt tcgtggactg acacaagttg ttctaggagg
    18421 ccaatctcta gcaccttttc cagctgtttt ccaaaaaatg tctacgggtt atttaccaga
    18481 cttaagcgaa ggaagcagcc ttcacttctt gacattgatt ttaggaatga tcatcacgat
    18541 cgtgttagtt gttggtcaat ggcgaacaag aaatcgtcgg aaaaatcatt tatttgaagt
    18601 ggaatctcca acagctttct tggtaaaggc ggtatttaca gcagtcgttg tactaggaat
    18661 aacttatatc tttgcctctt atcaagggta tccgattatt ttgatcattc ttggtttgat
    18721 cgttgcagca tatgggtttt tgacaaataa aacagtagca ggtcgacaga tttatgcgac
    18781 gggcggaaat cgaaaagcgg ctgaactatc agggatcaaa acaaagcgaa tcacgttctg
    18841 ggtattcgtc aacatgggag tcatggcagc tttagctgga ttagttttag ctgctcgatt
    18901 gaatgcggca acacctcaag caggtacatc gatggaactg gatgcaatgg cggcagtata
    18961 ttttggtggt gcttcgactt ccggcggcat tggaacaatc atgggtgcga ttgtcggtgg
    19021 cttggtcatg ggtgtattga acaatggcat gtcgatttta ggcgtcgggg tagactggca
    19081 gcaggcaatc aaaggattga tcttgctttt agccgttgtg ctagacttgt ataataagaa
    19141 aaaacgaaca acgtaggaga acgccggtta ttcagaaaaa agactgtgac aagacatttg
    19201 tcacagtctt tttttcgcta aaagcgaaca ttcattgttt cgtttgtata gactaaaaat
    19261 aatatatgtt tttttaccat tcgtataaat tctctttttg ctcttgattt ttttgcttaa
    19321 ttgagttaaa ttacataggg cgctaaaaaa agccttgatt aaattaataa tgttcgtgtt
    19381 ttgctggatg tgaacatcag gaggataatg taatgaaagt atttgattac gaagatattc
    19441 aattaattcc taataaatgt atcgtaaata gccgttctga atgtgataca acggtcacat
    19501 taggtaaaca tacattcaaa atgccagtag tgcctgccaa catgcagacg attattgatg
    19561 aaacaatcgc agaattctta gcggaaaatg gttatttcta tatcatgcat cggtttgatg
    19621 aagcagctcg aattccgttt atcaaaaaaa tgaaaaagcg tggattgatt acctcgatca
    19681 gtgttggcgt caagaaagag gaatattcct ttatagagaa gttggcagaa gaatcgctaa
    19741 atcctgatta tatcactatc gacatcgcgc atggacatgc taactcagtt attgatatga
    19801 ttcagcatat taaaaaatat ttgcctgaaa cttttgtgat tgccggtaat gttggtacac
    19861 cggaagccgt ccgtgaattg gaaaatgcgg gcgcagatgc aacaaaagtc gggattggtc
    19921 caggaaaagt atgtattaca aaaatcaaga caggctttgg gactggaggc tggcaactgg
    19981 cagctcttag atggtgtgcg aaagcagctc gcaagccgat catcgctgac ggtgggatca
    20041 gaacacatgg cgatattgca aaatctgtcc gttttggtgc gacaatggtc atgattggtt
    20101 cattgtttgc cggtcacgag gaatctcctg gcgaaacaaa agtcgaaaat ggaatcgtgt
    20161 ataaagaata ctttggttct gcttcagaat tccaaaaagg cgaaaaacga aatgttgaag
    20221 gaaagaaaat ctggatccaa cacaaaggat ctttgaaaga tacactagtg gaaatgcaac
    20281 aagaccttca atcatctatt tcttatgctg gaggaagaga tctggaagcc attcgaaaag
    20341 ttgactatgt catcgtgaaa aattcgattt ttaatggaga cacaatctaa aaaataaata
    20401 aaatattagg atgttgtgaa aaagttgtcc tgcattaagt aataagaaca gccaaacaaa
    20461 aatagttctt tatatgtttt gtttggctgg aattattatc gcagatacaa gcttttgaac
    20521 atcattattc agaataaggg actgtgataa gattt
//
LOCUS       DAIGOK010000045        20392 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.045, whole genome shotgun sequence.
ACCESSION   DAIGOK010000045 DAIGOK010000000
VERSION     DAIGOK010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20392
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.045"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(15..1154)
                     /locus_tag="NOA01_001291"
     CDS             complement(15..1154)
                     /locus_tag="NOA01_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HCI0632497.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
     gene            complement(1553..2851)
                     /gene="eno"
                     /locus_tag="NOA01_001292"
     CDS             complement(1553..2851)
                     /gene="eno"
                     /locus_tag="NOA01_001292"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCI0632498.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            complement(2967..3722)
                     /locus_tag="NOA01_001293"
     CDS             complement(2967..3722)
                     /locus_tag="NOA01_001293"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCI0632499.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            complement(3826..5016)
                     /locus_tag="NOA01_001294"
     CDS             complement(3826..5016)
                     /locus_tag="NOA01_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCI0632500.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            complement(5126..6127)
                     /gene="gap"
                     /locus_tag="NOA01_001295"
     CDS             complement(5126..6127)
                     /gene="gap"
                     /locus_tag="NOA01_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCI0632501.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            complement(6168..7259)
                     /locus_tag="NOA01_001296"
     CDS             complement(6168..7259)
                     /locus_tag="NOA01_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HCI0632502.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            complement(7408..8742)
                     /gene="rpoN"
                     /locus_tag="NOA01_001297"
     CDS             complement(7408..8742)
                     /gene="rpoN"
                     /locus_tag="NOA01_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCI0632503.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            8802..8873
                     /locus_tag="NOA01_001298"
     tRNA            8802..8873
                     /locus_tag="NOA01_001298"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8835..8837,aa:Arg,seq:ccg)
     gene            complement(9065..9949)
                     /locus_tag="NOA01_001299"
     CDS             complement(9065..9949)
                     /locus_tag="NOA01_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCI0632504.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNYTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(10036..10935)
                     /locus_tag="NOA01_001300"
     CDS             complement(10036..10935)
                     /locus_tag="NOA01_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HCI0632505.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            complement(10952..11458)
                     /locus_tag="NOA01_001301"
     CDS             complement(10952..11458)
                     /locus_tag="NOA01_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HCI0632506.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            complement(11647..12267)
                     /locus_tag="NOA01_001302"
     CDS             complement(11647..12267)
                     /locus_tag="NOA01_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HCI0632507.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            complement(12343..12780)
                     /locus_tag="NOA01_001303"
     CDS             complement(12343..12780)
                     /locus_tag="NOA01_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HCI0632508.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            12943..13773
                     /locus_tag="NOA01_001304"
     CDS             12943..13773
                     /locus_tag="NOA01_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCI0632509.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            13757..15124
                     /locus_tag="NOA01_001305"
     CDS             13757..15124
                     /locus_tag="NOA01_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCI0632510.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            15143..15952
                     /gene="yidA"
                     /locus_tag="NOA01_001306"
     CDS             15143..15952
                     /gene="yidA"
                     /locus_tag="NOA01_001306"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCI0632511.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(15995..16438)
                     /locus_tag="NOA01_001307"
     CDS             complement(15995..16438)
                     /locus_tag="NOA01_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCI0632512.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            16572..16943
                     /locus_tag="NOA01_001308"
     CDS             16572..16943
                     /locus_tag="NOA01_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCI0632513.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            complement(17112..17894)
                     /locus_tag="NOA01_001309"
     CDS             complement(17112..17894)
                     /locus_tag="NOA01_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HCI0632514.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(17891..18862)
                     /locus_tag="NOA01_001310"
     CDS             complement(17891..18862)
                     /locus_tag="NOA01_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HCI0632515.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            18954..20348
                     /locus_tag="NOA01_001311"
     CDS             18954..20348
                     /locus_tag="NOA01_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCI0632516.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
BASE COUNT         5721 a         4212 c         3635 g         6824 t
ORIGIN      
        1 ttattaaaaa aatcttacgc taagtaggat aaaataattc cttgatattt ttcgatcata
       61 tccaaataat tatcgatttc aacatactca tctacttgat gaggcaaagt aacgacacca
      121 ggcccaaaga cgacaaagtc gaaggaatgg ttagctttcg taaattcggc tgcatccgtt
      181 gttgctgccg ctccaactaa aggtaatggc tggctgaatt gttgctgaat gcaatgaatc
      241 aaaggagaat ctggatcagc tttgacaggt attttgttgt aatcgatcat caattccaaa
      301 tgatagtctg tctcttgatt caattcattg acgattgatt gcagcaatgc aatgatttta
      361 tcatttgggt attctggaat cgatcgaatg ttcccttgga gctgcgcatg gcttgggatg
      421 ctgttgactt gattcccgcc actgatcaat gtaacattat ggattgtttt tcctaggaca
      481 ggattctcga ttgtttcagc gaggtgattc atttcagcat ttgctttcgt aatgaattca
      541 tttaggtgat tgattgcatt ataaccttgg tctggcatag agctatgcgc ttcttttccg
      601 tgcgaggtga ctgtatagtt gattgatccc atgtgcgtat acatcaaact gtaattggtt
      661 ggctctccga tgatcaaggc atctaagtca tctacatagc ctgctttcgt gagctgttcc
      721 ccacctagtt cacccacttc ttctccgaca gtggctaata atttgactgt tccgttaaat
      781 ggttttcctg actctttcag ttcgatcatg gcaatcacca ttgctgccaa gccactcttc
      841 atatctgtag caccacgtcc gtacaaacgg ttcccatgga tttctgctgc aaagggtgca
      901 taggtccaag acgattcatc accagccgcc acgacatcca tatgtccaga taggcctaac
      961 acttttcctg attggccctt ttggtaacga gcaatcaaat tatctctacc atctctgtaa
     1021 gaaacaatct ctcctgttat gtcatgcttt gccaaaagct tattcaaata ggctgctact
     1081 tcaccttcgt ttccattcac tgatttaatt cggataatct cttgtaaaat cgcgattttt
     1141 tcttcttttt tcatctaatc ctctcccttg ccatttcttt ttattgtatt tgagtgtgct
     1201 cgaattttca aataggagca accaaagtaa aaatgaagaa agagagccac tttatgaaaa
     1261 atatgctgct acttccctcc tattttattt atacgagagg acttgcccat agattcctat
     1321 tctactgttc tatccttaaa agctcttata tgatgctagt tattgttagc tttccttttt
     1381 caaggatgat cttttcttgt accattcttt gtaaacatca tatacagaag ctagttgcaa
     1441 aaaaactctc tacctgctga tatttcagca agtagagagt tcgattactt aactaagtga
     1501 tcatactcga ctatctttca atagtcgatc atgtatcttc attagaaaag aattatttgt
     1561 tttttaagtt gtagaaagat ttcaaacctt tgtattctgc aacttcacca agttggtctt
     1621 cgatacgtag taattggttg tatttagcaa tacggtcagt acgtgataga gaaccagttt
     1681 tgatttggcc agcgtttgtt gcaacagcga tatcagagat tgttgaatct tctgtttcac
     1741 cagaacggtg agatacaact gctgtgtagc cagcttcttt agccatttcg atagcttcga
     1801 aagtttctgt taatgtaccg atttggttca ctttgataag gattgagtta gcaattcctt
     1861 tttcgatacc ttcagataat ttttgagtgt ttgttacgaa caagtcgtca ccaactaatt
     1921 gaactttgtc gcctaatacg tcagttaatt tcttgaatcc gtcccagtcg ttttcgtcta
     1981 atccatcttc gattgagatg attggatatt tagaaactaa ttcttcgtag aatttgatca
     2041 tttcgtcagt tgttttttcg ccttcgcctg agtcagctag tacgtaaaca cctttttctt
     2101 tgtcgtagaa ttctgaagaa gcagcatcca tagcaagaac aacgtcttta ccaggtacat
     2161 agccagcttt ttcgattgct tcgatgatta cttcaaaacc ttcttcgttt gaaccaaggt
     2221 ttggagcaaa tccaccttcg tcaccaacag aagttgctaa tccgcgagct ttcaagattg
     2281 aagctaatgc gtggaatact tcagcaccca tacgtagagc ttctttgaat gttggagcgc
     2341 caacaggcat gatcatgaat tcttggaagt cgattgagtt atcagcatgt gatccgccgt
     2401 tgatgatgtt catcattggt gttggcaata cttttgtgtt gaatccgcct aggtagtggt
     2461 ataaaggtac ttctaggtag tcagcagctg cacgtgcaac agcgatagaa acacctaaaa
     2521 tagcgttcgc acctaattta cctttgttag gagttccgtc taaagcgatc atagctttgt
     2581 cgatagccat ttggtcacga acatcgtagc caatgattgc ttcagcaatg atgttgttta
     2641 cgttgtcgac agctttagtt acacctttac cgccataacg agctttgtct ccgtcacgta
     2701 attcaactgc ttcgtattca ccagttgaag cacctgaagg aaccattccg cggccaaaag
     2761 ctccgctttc tgtgtatact tctacttcga ttgttgggtt accacgtgag tctaagatct
     2821 cgcgagcgta aacatcagta ataattgaca tgttttgtct ctcctttgag tttgtttttt
     2881 cctaagggat ttccctcata tacgatttta gttaattttg ctcattagtg caaacatttc
     2941 ttgcattttt cgcaaatttt caacaattat tttacagcgt ctaacaatga caagaatgaa
     3001 tctgcttgca agcttgcgcc accgactaaa gcaccgtcaa cattttcttt tgccatgtat
     3061 tcagcaatat tttctggttt tacagaaccg ccgtattgga tacgaactgc ttctgatact
     3121 tcttttccat aaagtttttc aactgtttga cgtacaactc cacaaatttc gtcagcgatt
     3181 tgtgcgtcag ctgatttacc agtaccgatt gcccagattg gttcataagc aataaccatg
     3241 tttgacacct gttcagctga aagatcagct aaaccagctg taatttgtcc ttcgatccat
     3301 tcagcagttt tgcctgcttc ataggtttct aaagattcgc cgcagcatag gattggtgtc
     3361 ataccatttg caaagatagc tttcgctttt ttattgatgt cttcgtctgt ttcatggaaa
     3421 tattcacgac gttctgaatg tccgatgatc acataatgaa cacctagatc agcaagagct
     3481 gctggtgaag tttcaccagt gaacgcacct gagttttccc agtagcagtt ttgtgctgag
     3541 atttttagtt ctgtaccttc agctgatgtc attaattctt gtaagaataa tgcaggagat
     3601 ccgatgactg aatcaacttt gtcatttgaa gggattttgt tttttactgc ttcagcaaat
     3661 tcttttgctt ctgatgcagt tttgttcatt ttccagttac ctgcgataat aggtttacgc
     3721 attgaggaac acactcctta atttattgaa aaatggctag ggagtcatga aaactccctt
     3781 caccttttgt tcgattactc gtcagaattg agcaaacttg gtcgtttatt tatcgttgat
     3841 tgctgctagt cctggtaatt ctttaccttc aagcaattcc aagcttgcgc cgccacctgt
     3901 tgagatgtgt gtgaatttgt cagcaaatcc taattgttct gctgctgcgg ctgagtctcc
     3961 gccaccgatg attgttgttg cgccttctaa gttagcgatt gcttcacaaa caccgattgt
     4021 acctttagca aagttactca tttcaaatac acccataggt ccgttccaaa caactgtttt
     4081 tgcaccttgt aatgtatcag caaataattt tactgatgct gggccgatgt ctaaagccat
     4141 caaaccatca ggaatgtctc cttcgattac ttgtgtttca acatcatttg aaaattctgg
     4201 tgcacagaca ttgtcgattg gcaagacaag tttgtcgcct gctttttcga ttaattcttt
     4261 tgctagttcc actttatcag cttctactag agagttaccg atcttgattc cttttgcttc
     4321 gtagaatgtg taagtcattc cgccaccgat caagatttta tctgctttag gaatcaagtt
     4381 ttcgattacg ccgattttat cagaaacttt tgcgccacca aggattgcaa tcattggtcg
     4441 ttttggttct tctactgctt caccgatgaa cttgatttct ttttccatca agaatccagc
     4501 aactgttggg ataccagttg aagcgatacc tacgttagaa gcatgtgcac ggtgagctgt
     4561 accgaaagca tcgttaacaa atacatcgcc taatgaagcc cagtatttac ctagttctgt
     4621 atcatttttg ctttcttttt taccgtcaat atcttcaaat cgtgtatttt cgaatacgac
     4681 aacgtcgccg tcattcatgt tgcggattgc ttcttctaat tgttctccgc gagtttcagg
     4741 tacgaatgtt acttctttac ctaataattc gcctagacgt tttgcaactg gagctaaaga
     4801 ttttccttct ttatcttctt ctgtttttac acgtccaagg tgagagaaaa ggattgcttt
     4861 tccgccttgt tctagaacgt agttgattgt tggcaatgct gctttgatac gagtgtcgtc
     4921 agtgatcacg ccgtctttca aaggaacgtt gaagtcaaca cggacaagaa cttttttgtc
     4981 ttttaaatcg atatctttca ctgttttctt agccatttta tttcctcctg aataaactta
     5041 ttatcaaaaa aagcggaaga agcgcgattg cttccccgct tttaaatgta catgtctata
     5101 atttacactt gtacgtttgt ctatcttata agttagcgaa gtattctaaa gtacgtacta
     5161 attgtgcagt gtatgacatt tcgttgtcgt accaagcaac agttttaact aattgtttgt
     5221 cgccaactgt cattacttta gtttgtgtag catcaaataa tgaaccgtaa gtcataccaa
     5281 cgatatctga agaaacgatt tcgtctgtgt tgtatccgta agattcgttt gaagcttctt
     5341 tcattgctgc attgatttcg tcaacagtta cttctttttc aagaactgtt actaattctg
     5401 ttaatgaacc tgttggtaca ggaacacgtt gagcagctcc gtctaattta ccgttcaatt
     5461 cgggaattac taaaccgata gctttagcag cacctgttga gttaggaacg atgtttgcag
     5521 cagcagcgcg tgcacggcgg aagtcacctt taggatgagg tccgtctaga gtcatttggt
     5581 cacctgtgta agcgtggata gttgtcatta atccttcaac aacaccaaat ttgtcgttca
     5641 atgttttagc cataggagct aaacagttag tagtacatga agcacctgag ataactgttt
     5701 ctttaccagt taatgtttcg tggtttgtgt tataaacgat tgttggaaca tcgtttccgc
     5761 caggagcaga gataacaacg cgtttagcac cagctgttaa atgtttttca gctgctgatt
     5821 tagaagtgaa gaaaccagta cattctagaa cgatatctac gcctagttcg ccccatggta
     5881 attcttctgg gttgcggtta gctaatactt taacttcttt tccgtttacg ttgaatgaac
     5941 cttcatgaac ttcaactgtt ccgttgaaac gtccttgagt tgtgtcgtat tttaacaagt
     6001 gtgccaacat tttagcatct gtcaagtcgt tgattgctac tacttcgatt ccatctacat
     6061 cttggatacg acggaatgct agacgtccaa tacgtccaaa accattaata cctactttaa
     6121 ctgtcattta agatttcctc cttatgaaaa tcaaaaaaat atttatttta aagggttacc
     6181 ccttttaaaa tctcatttgc agcagcttca tcagtgatga gccacgtttg ttttggtgca
     6241 tttttcatat aagctcgaat tgctttagct tttgattttc cgcccgcgat tgccatgatg
     6301 atcgggactt tttccaaatc ttgcaattct atgccgattc tagggacctt ataaacgact
     6361 tcaccttttt cattgaaaaa gtagccaaaa gattccgcaa cagcatttgc ttgtttcagc
     6421 atgactaatt ctttttcagt catcttacga cgtgctgcca tgtgtaatgc acgcccaata
     6481 ctatgaataa cacaattcgc ttcactaatc aagtttaaga cttcttggat agatggctca
     6541 ttgagtaaag aattgtaagt tgccagactt aattgttccg gaacataaag tgcgcggtgg
     6601 ttaccgtttg ctttagcagc cattcttgca ctgacagaat ttgcctgtac agttatcgct
     6661 tcaccaatcc cgcctcttgc agggacaaac aggttatgcc gtttcttatt ttccagatta
     6721 gacaattgtt cggctaccac agccatagta gttcctccca tcacagcgat gatattctcg
     6781 ccttctggaa gtagtcggtc taaggcttcg ctcagcgctt cgccgaatgc atcagccact
     6841 ttggtctgct cttcgctgtt tcctgaaaca acgatacagc gttggttacc aaaatattct
     6901 gccaactgtt gttcggtttg atgcgtacct aaaagctgat ccatgaagtt ttcaaggctt
     6961 tgataaactt cttcgccctt agttgtcagc gtcataccgc tttttgaggt gtcgatcaaa
     7021 ttcagatttt tcaaaaggtc tgtttctgtt cgtaagactc gttctgtgat ccccatactt
     7081 tccgataaac ttcgacgccc aatcggctgc atccaataga tatttcgtaa aattttatag
     7141 cgttcttgca acacttcgat aatatctggt gcaacagatt caatcatttt tatttcatcc
     7201 agcatagcga tctccttagt tggacacttt acgtccaatg tagacttaaa acgacccata
     7261 tacgataaaa aaagaaatta actattcgaa tttcacatca ataaagggaa atctcaacct
     7321 cacgactgta agtctaacag tttcgctctc gtttttcaac taaaaagact agaaaaagtg
     7381 aaatactttt tctagtcttt tgactgatta gttatcataa cgttttcttt ttgatgaact
     7441 cgggattcct aataaatcac gatattttgc tactgttcgt cttgagatcg ctatctcatc
     7501 ttttttcagc aattcaacca atttctggtc cgacaacggc ttgtttttgt cttcttgatc
     7561 gactaattcc tgcagctttt tcttcgctgt atctgctgaa aggtcttctg tctgttctgt
     7621 actattcgta ggaattcgtg tagtgaaaaa tttttttaat tcaaatacac cgaaatctgt
     7681 ttccaaatat ttcccgttga ctgcacgact gaccgtggat tcatgcacat ctatctcagt
     7741 tgcaacttcc tttaacgtaa gaggcttgat tggacgttcc ttgtcgataa agaaagcttt
     7801 ttgccgggaa acaatgactt gagcaacccg caaaattgta tccccacgtt gtaggatcgt
     7861 tttcttcaac cattcgaatt cctgtttccg ttcttttaaa taattttcag tttctttatc
     7921 tgcttgttgt gacatttgtt tgaagtaact ctcttgaaag cgtatatttg gctgattttt
     7981 tcggtttgag ataacttgta catttttatc gtcgtccact aatacccgga catcaggaat
     8041 aatgtacaat tcgctgctac ggtcgaaaat ccggccaggg cttggactca acgtttgtaa
     8101 atagtcgaat atctgctgaa cagcatgtag atcaacagaa aaacgttgtg cgatttcttt
     8161 ccattttcgc tccaccagtt cctcaaaaca ttcttccaac acgatataag ctaattccgg
     8221 cgcataatca tctcgttctg tttgaagcat gagacattct tgcaaactac gtgcacctac
     8281 tccagctggt tctaattgct gaatcagagt caacgcatcg agcatttgga taggcgtagc
     8341 tccaatctct tttccggctt cttctaaact aatcgtcaaa aatccgttca aatcgatgta
     8401 ttctacaaga taaagaacga ttttcctcaa aaaagtatct cggtaattca aatggacttg
     8461 ttcgatcaat gaatcaaata aagacccttt tgtatcagga atctgattta ggtaatttgt
     8521 ctcttctcct tcataagaag atgaagaagc ttttatgtaa tctggctgcc attccggctc
     8581 aacaacttcc accaatggat tttccattgc ttgattctca acgaatgcca acagctcctc
     8641 actgttatat tgtaaaactt ggatggactg ttgcaactgt tgcgtcatgg ctagtttctg
     8701 tgtctgctgc tgtttttgtg aaaaattttg ctcaaatttc atttttttgt ccaatcccct
     8761 tgtaattttt tatctgttta gttacaatta tcccagtgct tgcgtcctta gtgtagtgga
     8821 tatcacgtaa gattccggtt cttgagacgg gggttcgatt ccctcaggac gcgttctact
     8881 gctattttag cataatttac gaatagttgc ttctttattt ttaagtgaaa actgtccgcc
     8941 aaagaaaatt ggtggacagt tttttgttgt acctgtatag atattgttaa gcctttgcaa
     9001 gtaaggtagt tatgtgttca actacttatt aaaatgtgtt atttattaaa gatacttgtc
     9061 tcacttactg aactggccga tgatcgtttc tgccataata gttctcgaca gccgttggat
     9121 gccctaataa atccccaact gtcgtgatgc gatacccttg ctctttcaga taatcgatca
     9181 cttgaggaac ggcacgaatc gtttcaggat agatatcatg gaataaaata attgccccat
     9241 cgtatactgt tgcttggatc ttctgcaaaa tcaaatcagg atttcttgtc ttccaatctt
     9301 ctgaatcaac agaccattcg atgatcggtc gctggatgat cgttgctcct acattcgtga
     9361 cagaaccata gggcggacga acaaaagtgg gggttttgcc agtgactttt ttgatctcag
     9421 tgtctgcttt cgtcacttcc tccgtcatcg actgggcaga aagagtggcc agattctgat
     9481 gatcgtatgt gtaattgcca atctcgtgcc ctgcttcagc tgtctgcttc acgatttcag
     9541 gatgctgcgc cgtattttct cctaagacga aaaaagtggc atgcacgttt ttttctttta
     9601 agattcgcag aagctgcggt gttgtggtcg ctcctggtcc atcatcaaag ctgattgcga
     9661 cgcttttgac agccggatct cggctcgttt ttgtactgtc ttgtgttgat tgtgatgact
     9721 gactttgtac cttttccgct gattcttgaa tggcttcttt gacgttagct gtttcagttg
     9781 attggctttt aatggttgat tgttctgatt gtttgaaaga ttgtgacgaa tgctccgaag
     9841 agtgtgtttg tccttttact tcggtgatca aggaggccac cgaagtaatt tcgaaaaaca
     9901 aaagcacgga taaagcaata atactgataa ctaaataatt tcttttcact atatctcttc
     9961 tttctctttt tctcttctta ttatttactt gtacttactt gaaaattgaa actgctagtc
    10021 gcaactactt ctcgtctaat gatccaaata atcttccgat gccaaaatcg tttttgtcac
    10081 ggacttagga atgacaaatt ctggtgtacc catataacca ggagcaattt cgtattgaga
    10141 aaaagtcaaa actaattgat gctctttgtt caaataaaaa cttcgtgtag gcgtaacaag
    10201 tgttggtttt tctatcgtcc cttcttgaat atcttgctca gtccaatagt acttagatgg
    10261 atcttccttg acttgttcgg ccatctgccg tttgacttct tttgtcaaaa cagatagata
    10321 ggtgtcatct ttgaataaaa gtggaagtga gagctggaca ctgtttttct tgtcgattgt
    10381 gtcgtatctt ttttcaacat ggctatctgc tgctgtttgg gtgatctgcc tttcaatcac
    10441 gagaaaccgg ttatcgtcta cgattttctg ataatctccc aaaacttggt aatgatccgt
    10501 ttcaaaattt ccatattctt tttcagcttt ttcaaaatct gcttgtcctt cgcgaaagta
    10561 tttttgattt aatccatgaa ttgttttatt ttctttctca ttttgtgtat tgatcttcgg
    10621 tacctgaata tcgatttttt cagatcgatc agtaaagcgt tcgcctaaga tcaattcaat
    10681 gactgatcct aagatgggta aatcttctgc aaagcttcga acttgactat taaaaagaag
    10741 gctacttgtt gtaatcatta cgatagccaa agatactccg aacccttgga gacgtctctt
    10801 tctccgcttt gcttttataa atgattgctc ttcgataata aaacgctggc ggatctcctc
    10861 tttaagccct ttgggaattg cttgttcttt ataagttttc tttagttttt ttaaattttc
    10921 tttttgggaa tacatggcac tcttctctcc ttcattctag gatttgacgt aaagcattca
    10981 atgagcggta cattttcgtt ttaactgtgt tttcattcat atccaagata gtcgcaattt
    11041 ctttcaaacg aaatccttca aaaaagtaga ggatcagaat ctctttttcc ggactgtcta
    11101 ttttatctaa aatcatatgg agctctgcaa cggaagtttc taaaggtaac tgttggggga
    11161 tatcttgaat ttcaaataag tccacaggcg tccttttttg tctgcgccaa ctatctaatg
    11221 ccgtgcggat caatatccga taaaaccatg cactaaaatg ctcgatctct ttttcttttt
    11281 caaaatagtt cagggcttta tgaaaactat cttgcatcac atctaaggcc tcttctttat
    11341 catgtacgta attcatcgtg atacggaaca ttttttgata atcagtttcg atgagtccat
    11401 tcagcaggtg acggtatttt tcgatctgcc gttttttctt atatccagta aaaatcattc
    11461 tccactcctt tctgcctcac ttaatatgac gtacaaggtg cgctaaaagt ttcaattttt
    11521 ttatttttta tgtatttact tgggattttt atatacaaaa aagaaggtgt gacaaaaatc
    11581 gtgtcacacc tccttattct gataggctga tcgtctctgt cacaacctct cgtaagaaaa
    11641 taatgtttat tctttttcag agtcagaaaa ttcttcctct tcataatctt catcatcatc
    11701 gtcttcgtct atgatactta aatcttcttc gatatttcct ggcaagtctt cttcatcatc
    11761 tgtcgggtca tctgctccga tttcttgcaa gtcagaatta taagcagcga tttcttcatc
    11821 ttcatcatca tcgtcatcaa acagatcatc gtcatcttct tctgtcagat ctgtatcttc
    11881 tgggtcatcg tcattgtaat cgatcgcgtc ttcatcatca ttgataaagg cgttgacttt
    11941 cttgcgttta cggcgacgtg gagtatcttc atcctcttcg tccattccat ggtttacttc
    12001 ttcatcgatc gaatcgatag cataccatga acgcaagccc caacgattat cccctaatga
    12061 gataaagctg ccgtcaatat tcaagtcagt ataaaattgt gcgagtgaat cacgaatttc
    12121 actgtctgat ttcccaagat agttttggat ctggttagct aaatcagaaa aatccatgac
    12181 atcgccacgt tgttctaaga tcgcgtgtgc tacttcgatc attgataatt cgcttttgtt
    12241 tgcaccttca aatacactaa tttccaatca ggacacgtcc tttcacagtc tactccttat
    12301 catacaaaat cagacacaaa aaatcaaact tttcttttat atttacgctg taaaatccaa
    12361 ggtcaaataa tattcgccga ttttttccgg tcccatgaac agatcataat cgattcgaac
    12421 cttccctcct gtgggacggt ctttcaaact aacatgcagt tctttcgtaa aagtagcaat
    12481 ctggatcata ccgtatggtg ttcgatagga tgtttctaac cgttctttat agccaaattt
    12541 caaacgcatc cgcatttctc ctgcacggat cagctgtaca tgaccatctg gaactaattt
    12601 gatcgttaca ggaatctcat cgcccgtctc ctcctgaatc tctttataac gtatgtacag
    12661 cgtatcgcca attgtcacca tttgcccttt caaatcaaaa taaaaatctt gcacttcccc
    12721 ttgctgtttt acttttgtac gcaatttgat tgaaatcggt gttccttgtg ataattccat
    12781 tattcattct cctttacttc tcatagctac attgttccta gtttcttttg ttttgtcaac
    12841 caacttgtcc atttttacag aaaacctttt ttccaaaaca actctttgat tttagagtat
    12901 aatattgtag aaaatatgaa tttatacaaa ggagccggtt gaatgaaccc tgcgattcga
    12961 ctagatcagc acatttatca aaaaaacgac ttttcccctt ttgcattaac agatatatta
    13021 acggaatatt ctaaagaaca ccagcaaatc tttttacatt tctggcaata tgactgcaca
    13081 gtcatcttag gtatgaagga tgttcgaact ccttttctaa gtgacgggat ccaaacactg
    13141 aaagatgcag gttacgctcc agtgatcaga aattcaggtg gattagggat cgtatcagat
    13201 gaaggaattt tgaatatttc tttgattttc ccccaaagtg acacaaaaaa aataaccata
    13261 gatgacgctt acgagcaaat gctcctattg actaaatacg cttttccaaa ggcaccgata
    13321 caagcattcg aaatcaccca ttcttattgt cctggaactt atgatttaag catccatggc
    13381 aaaaaatttg ccggtatcgc tcagcgtcgg atcaaaaatg ggatttctgt tatgatgtac
    13441 ttaagtgtca acggtaatca ggaaaaacgc gggcaagtca tgaagcgatt ttatcaatct
    13501 gctttaaaag aagactttgg tacaaatggc tatcctgcag tggatcctgc tacaatggca
    13561 acacttgaag aactgcttga ccaacccctt tttgtaaaag aagtcaaaga agcatttgca
    13621 aaagcttttg aaaagctgta ttctgcaagt gatttacagg attctaccga ctggctaaaa
    13681 aaagaaatcc agctggagca atggaacaac caaatccaga gaatggaaca acgtaataac
    13741 ataaaggagc tacattatga caacccctta taaatatcaa atgctgccta tggaaaaagt
    13801 ttttcgtgat ccagtccata actatatcca tgtccagcat caagtaatct tagatttgat
    13861 caattcgaaa gaagtccaac gtctacgacg cattaaacaa ttaggcactt cttctttcac
    13921 ttttcacgga gcagaacata gccgttttac tcattcatta ggcgtttatg agatttcaag
    13981 acgaatctgc gatatctttt ctcgaaattt ttcaaaagaa aaaataggca acggaggctg
    14041 ggatgatcag gaacggctgg tgactttatg tgcggcactg ttgcatgatg tcggtcatgg
    14101 tccttactct catacatttg aacatatctt ccatacgaat catgaagcca tcactgtaga
    14161 gatcatcact tctccagaaa cagaagtgta tcagattctt aaccgagtag aagaaggttt
    14221 tcctgaaaag gtagctagtg tgatccaaaa aacttacccc aacccacaag ttgtccaaat
    14281 gatttctagt cagatcgatg cagatcgaat ggactatttg cttcgggatg cttatttcac
    14341 tggaacagaa tatggaacat ttgatcttac ccgaatatta cgtgtgattc gtccttatga
    14401 aggcgggatc gctttttcca tgagtggcat gcatgcagtg gaagattata tcgttagccg
    14461 atatcaaatg tatgtgcaag tgtacttcca tcctgtttcc cgtggtatgg aagtaatttt
    14521 ggagcatctg cttcatcgcg cacacgagtt gtatcaagaa gatgccagca gttttgaact
    14581 ccattctcaa ttacttctgc catttcttga cggaagcttt actttagaag aatacctcaa
    14641 acttgatgat ggggtcttgg gcacttactt tactcaatgg tctgaatctg ctgatcctat
    14701 tttaaatgat ttagctaaac gttttttgaa tcgtaaacca ctgaaatctg ctactttttc
    14761 tggcaaccgg gattcaaaat tagtccaaga actcactttg ttagtagaaa aggtcggata
    14821 taatcctgtt tattacactg ctgtgaattc tagctacgat ctaccttatg atttttatcg
    14881 cccagaacaa ggcagacatc gcacacaaat cgagattttg cgaaatgacg gaactttgat
    14941 agaattatca caggttagtc agcttgttgc tgcattagcg ggacaagaac aaggagatga
    15001 gcgatttttc tttccaaaag aaatggttga tccgtccttg cgtgatcatt atgatttatt
    15061 tgacgaaact tatcaagaat ttgccagcca tatccgtaat ggcgcattga ttgaaataaa
    15121 ttaaaatata ttggaggaaa ttatgtctat caaactagtc gctatcgaca tcgatggtac
    15181 tttattaaat gagaaaagag aagtaacaaa agaagtaaaa gaagcgattg ccgctgctgt
    15241 tgcaaaaggt gtttcgatcg ttttatgcac tggccgtccc ttaccaggtg tccaggaaca
    15301 gttaaacgag ttgaaccttt ttcaagataa tgattacgtt attacttata acggggcact
    15361 cgtacaacaa acgaaatcag gtaaaatcat cgcccgtcac ggactgacac atgaagattt
    15421 cctagagatc gaagtgatgg cacgccgtgt cggcagtcat ctccattcga tcgatgacaa
    15481 agcgatctac acgcctaatc gagatatcag cgcttatacg atccacgaag caagtttagt
    15541 caagatgccg ctaaaatatc gaacacctga agaaatgaca ccagacatga acatcgtcaa
    15601 aatgatgatg atcgacgaac cagaaatttt agatgcagca attgcgcgtt taccgcaaac
    15661 tttccgtgat aaatacacaa cggtcaaaag tgcgccctat tacttcgaag tactaaataa
    15721 agaagcaagt aaaggtgcgg cagttgcgaa tctcgcacag caccttggta ttgatcaaga
    15781 tgaaatcatg gctatcggag ataacgagaa cgacttatcc atgattgaat atgctggtct
    15841 aggagtagca atgggaaatg ccgtttctct tgtcaaagaa gcagcaaatg tcatcacttc
    15901 atcaaacgac gagcacggtg tagctgaagc aatcaaaaaa tatgtactgt aataaataaa
    15961 aaaactgaga gaaagctgat ctactcgctt tctttcagtt tttatttttt tcgatccatc
    16021 tctcgactgc ccatcggtgg ctaccacgtt tcaatttcac aagcgcttct tgagggctca
    16081 cccaccaaat tttattggtc ttttcagttg gttctcctat acgcttccaa gaatcagcaa
    16141 caaagaaata tcctggatta taataataag ttgcacgata attcgagtaa aaatactcat
    16201 cagcttctcc cagataagaa gaaatttctg ccacaattcc catttcttct atcagctcac
    16261 ggacaatcgc ttcttctttc gtctcttctc cttcaatttc cccgccagga agaaaaaaag
    16321 caccatttgg agcctcgatt actgcaatct gctgatctct ttctatgaca atatatgctg
    16381 cataacgggt ttgataatca gcaaattgct ttttttcacc aaaaattggt ttatccatga
    16441 atagtttcca ccttaacagt tttattttct atccatttta catgagaaat ttctcatgaa
    16501 caagcacaaa tcaactattt ccttacttct ttttgttatg attgagtaaa aggaaaagga
    16561 gagatgaatc tatgaaaatg gctcacacat gtgttcgagt gaaagatctt gaagcatcaa
    16621 tcgaatttta tcaaaaggca tttggttttg aagaaagccg ccgccgtgat tttccagaaa
    16681 acaaatttac acttgtttat ttgacacttc ctggggatga ctatgaatta gagttgactt
    16741 acaactatga tcatggtgcg tatgatctag gagacggcta tggtcatatc gctatagcag
    16801 ctgatgatct agaaaaatta cacgagcaac ataaagctgc cggtcttgag attactgact
    16861 taaaaggatt acctggcact gcgccttctt actactttgt cattgaccct gatggttata
    16921 aaatcgaagt catccgagga tagttagtat ggggcatttt actcttgatt gaataaaaaa
    16981 caaacaccaa taattaaaat gagacgcaaa cttttcatta aattgaaaaa gtttgcgtct
    17041 ttttttaaca accattgtag ctataaggtt gccactctcc accaatttat ttttaattca
    17101 tttgaaagaa atcaaataaa cgaggtacct ttagaaaaca aaactgcttt tcctcctatt
    17161 atcacacgat cttcttttaa ttcacattct agatccccgc cccttgatga catttgataa
    17221 gctgacaatt ttgttttatt cattctttct gcccaataag gaataagatt agaatgtgct
    17281 gatccacata ctggatcttc attgattgtt aattttggaa agaaggttct agaaacaaag
    17341 tctttttgca cactttctgc tgtcacgatg actcctattc ctaattcaaa atttttgata
    17401 gctgtaaaat ctggctgcaa tttagccacc gttttttcat cttctaatac gaaaaataaa
    17461 tcacgtgcta agtaggcttc tttaatctta gccccaatcg cttcttcata ttcagacaat
    17521 atcggcactt ttttcggcat aatactagga aaatccatgt aataataatc tttaattctt
    17581 gtcacaaata gattaccgct ttgagtagaa aatttaatcg tttcatcagg tattttgtaa
    17641 tagttaaata aaacaaatgc tgttgctaat gtcgcgtgac cacaaagatc gatttctcga
    17701 tctggcgtaa accatctaag ctcaaattct tggtttttct tcactgtaaa tgctgtttca
    17761 gataaattgt tttcaatggc aattttttgc atcgtacctt ctggcagcca ttcttctagc
    17821 acataaaccg ctgctggatt tcctttgaac acctcatctg caaatgcatc aactacgtaa
    17881 taatctaagt tcattttaaa gcccctttct ctaatttgtt ataaatatat tctgcacaac
    17941 aaagatcttc aatagctaga ccaactgcgt cgaaaatcgt cggcctcgat tctcctttat
    18001 aaaatgtttt tttctcagaa actaattcgc ctaatgtacc aacaatacaa gattcctcta
    18061 aatctccttc tgccatggct ataagcaggt ccccgctttc tgttaaggca gctgagtaat
    18121 catctacata gatttgagaa gtttccatca acgatgtact tatctctctt gtatcaggag
    18181 taaatgtgcc cacagcattg atatgtgcgt cttcccttac ccactctttt tgaagaaatg
    18241 cctctttact agaggtcaac gtacaaatga tttccgctgg acgtactgca tcttctactg
    18301 atattgcatt tataaacgtt attttaggat actgtttttt agctttagcg ataaaagtat
    18361 ttcgtctttc ttctaaaata tcgtaaacaa acactgtagt gatgggacga atggtagaaa
    18421 tcgcagtcaa atgcgacaat gcttgttgac cagctccaat taaagcaagt gtttctgcat
    18481 gcggatttgc taagtaatct gttgccagcg cagatacagc agctgttcga atccaggtga
    18541 ctgcatttgc gtttatcaat gctttgggta gtccgttttg agcatcaaac aagaaaatct
    18601 ctcctaaatg agaaggtaaa tgatactgat gattatccgg aaaagcggta atgacttttg
    18661 tgccaaaata tctgttctcc cccaaataag caggcatcat cgcaaacata tttttgcctt
    18721 ttccgtcagg taaaatttcc accaatcgag gattttggct gattttacca ctttcataat
    18781 caaaaaaaca ttttttcatt acttgaatcg cttcttcaaa actaaaagaa gctactactt
    18841 cctcaaaatt aataattttc atagacaatt ccctcctcac aaaagattaa cttctaaaat
    18901 caatacaatc aatggtaaaa ttgtatggta tacaatttta aggagtggag aaaatgccta
    18961 ttaattctta tgacaattac ccgttaactt ggcgaccaga caaacaaaaa ttgaagcgtc
    19021 cttattatcg atcgttgatt caacaattag aacaagatat tttaaagaat aaattacaga
    19081 aaaacactca gttaccctcc caaagagagc tagctgatta tttagattta aattttacta
    19141 ccgttggcaa ggcttataaa tatggaattg agaaaggttt gctgtatacg aatattggaa
    19201 gtggcacatt tatttctccc aatgtctttg agtccatcac catctctacc aatgaagtaa
    19261 ccgaacatat cattgatttt ggtctggtta gttcttttga agaatgtaat aaattcgttg
    19321 tcccgtttat tcaatcaact agtaataacc caaatcttgt tggtttgctt aattaccaag
    19381 atcccaaagg cacaaatttt caattgacta ctgcagcaaa ttggttaaaa actcaaggag
    19441 tcattccgtc cttagaatct ctatctattg tttccggtgt tcaaaatgga ttggctatca
    19501 cactttctgc acttttctct ccaggtgata gaatagcaac agaccgatat acatacgcta
    19561 atttcattga attggctcag ttatatcatt tagagatcgt tccaattgat tttgacgagc
    19621 aaggaatgcg acctgatata ttgattcagg aatgtagaaa aaagaagatt catggtgtct
    19681 ttcttatgcc ttcttgtaat aatcctatag ggtttcaaat ctctcacaaa agacgagagc
    19741 aattagccga agtgattgaa aaagaaggtc tatgggttat tgaagatgat atccattcat
    19801 ttttaacaac ttactatcaa catacgatca ttcctccttt ccaaaattta ttacctaacc
    19861 aaacaatcta cttagctgga atgacaaaat ttatttgttc tggattgcga gttgcttatc
    19921 ttgtagttcc agtacatgtc caaagcaaga tagaacaagc aatttttaat ataaatgtga
    19981 aaacttctgg attagatgtg gaagtcatca ctcaaatcct ccattcaaat gaagcaaata
    20041 ggatcttaga aactaaatta agtcttacaa aaagagccaa tcagcttttt gattctcttt
    20101 tcaaagtaga aaaaccaacg aatcgtgtcc cttactatcg aattcttcca gttgatcctg
    20161 agattgaccc taagataatt gagaatgact ttcttgctaa cggtatccga ctttatcatt
    20221 ccagacgctt tactacacgt gcgcaaccag atgcattcgt tcgaatttcc ctatcctcta
    20281 ataaccttga aattttagct tctggactaa gtatcgtaaa agaaaaaata aaaaaatatg
    20341 aattataacc tattgataaa taaaggaatc aaatcttatt gattcctttc aa
//
LOCUS       DAIGOK010000046        20110 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.046, whole genome shotgun sequence.
ACCESSION   DAIGOK010000046 DAIGOK010000000
VERSION     DAIGOK010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.046"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            4..2376
                     /locus_tag="NOA01_001312"
     CDS             4..2376
                     /locus_tag="NOA01_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632517.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAESGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVTKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKTGQTELQVGIQAVAKNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFSLGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="NOA01_001313"
     CDS             2437..3897
                     /locus_tag="NOA01_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCI0632518.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERSNGTYDFDRFYQGKGDINAQILFSYKAEQYYKGYKKHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3908..6112
                     /locus_tag="NOA01_001314"
     CDS             3908..6112
                     /locus_tag="NOA01_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HCI0632519.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFNVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSLLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESYRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYHKAAACTSEACDFRLWTTIAKKKLTATQLKEII
                     QNGRTSQPVKGLKGQKGSFEATIVLKEDFTTSFEFSEKKKTNYKKRTRRTTK"
     gene            6128..6277
                     /locus_tag="NOA01_001315"
     CDS             6128..6277
                     /locus_tag="NOA01_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632520.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6358..6954
                     /locus_tag="NOA01_001316"
     CDS             6358..6954
                     /locus_tag="NOA01_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HCI0632521.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYSKAEWYELGKIEIKSRYGNPVIAYDPERTLCDIWNPWYNVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTIRSYIMALN"
     gene            6967..7866
                     /locus_tag="NOA01_001317"
     CDS             6967..7866
                     /locus_tag="NOA01_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HCI0632522.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNDIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFENRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYEETMDSISNLMEKVI
                     HVEEKERKIAYQRHQQMNREMER"
     gene            7869..8354
                     /gene="ssb"
                     /locus_tag="NOA01_001318"
     CDS             7869..8354
                     /gene="ssb"
                     /locus_tag="NOA01_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCI0632523.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGILIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            8720..8980
                     /locus_tag="NOA01_001319"
     CDS             8720..8980
                     /locus_tag="NOA01_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632524.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9427..9633
                     /locus_tag="NOA01_001320"
     CDS             9427..9633
                     /locus_tag="NOA01_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HCI0632525.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9633..9857
                     /locus_tag="NOA01_001321"
     CDS             9633..9857
                     /locus_tag="NOA01_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632526.1"
                     /translation="MKTQHKLLNVKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFE
                     DLVKIAGYELHYQENLETYENEYWLTKLEN"
     gene            9872..10309
                     /locus_tag="NOA01_001322"
     CDS             9872..10309
                     /locus_tag="NOA01_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632527.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEAGVKPSTIKPSEYQALE
                     LTYGKFIETKKAKTVSSEWLDYFENVNKYGACYIMKKEDNENE"
     gene            10302..11009
                     /locus_tag="NOA01_001323"
     CDS             10302..11009
                     /locus_tag="NOA01_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632528.1"
                     /translation="MSNVVYQLTVEDVSHFLTNKEHLNEVVRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDCKRMPYQRFERKKGQ"
     gene            11390..11569
                     /locus_tag="NOA01_001324"
     CDS             11390..11569
                     /locus_tag="NOA01_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632529.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQELKQKYGKSESRIMCIALDLL
                     IAQEKAGFDVPALKK"
     gene            11662..11846
                     /locus_tag="NOA01_001325"
                     /pseudo
     CDS             11662..11846
                     /locus_tag="NOA01_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            11880..12311
                     /locus_tag="NOA01_001326"
     CDS             11880..12311
                     /locus_tag="NOA01_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCI0632530.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNLLRSGRNN"
     gene            13248..14522
                     /locus_tag="NOA01_001327"
     CDS             13248..14522
                     /locus_tag="NOA01_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCI0632531.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLAVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
     gene            14576..16003
                     /locus_tag="NOA01_001328"
     CDS             14576..16003
                     /locus_tag="NOA01_001328"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCI0632532.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPEDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDHLYMKPVRELEQLRVG
                     SGVETKVNLAGEIIIAQDASFKEALIDVLLTKSKNQEIKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            16016..16888
                     /locus_tag="NOA01_001329"
     CDS             16016..16888
                     /locus_tag="NOA01_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCI0632533.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNELLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            16983..17978
                     /locus_tag="NOA01_001330"
     CDS             16983..17978
                     /locus_tag="NOA01_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCI0632534.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKDN"
     gene            complement(18583..18690)
                     /locus_tag="NOA01_001331"
                     /pseudo
     CDS             complement(18583..18690)
                     /locus_tag="NOA01_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302409.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            18806..>20110
                     /locus_tag="NOA01_001332"
     CDS             18806..>20110
                     /locus_tag="NOA01_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCI0632535.1"
                     /translation="MIIKNLGNVKSIDIHVKDLLIFTGKNNTGKTYTSYILYGILSEL
                     FGARFFNYFSHTEIEPHLQNSLNILLVSKDSIRKKFNNDAKKFLEENLKKIAVRNFKV
                     SENNFKNLSFTISDSEISELTGDIFNYEIKRYSISGIVITIQNVGEKLKISFEPTDSF
                     QYLSKDIEIEDIYKILAQELSKNVISLPKVFYFPAERTGINVFKNELNESRLKTYDTL
                     LNTVQFTNLTNQKKKAALKYDLLKKNIELLMETTDNSSYPKPISDYINYLNNMKNRYE
                     QKNVNNIASYIRDEIIMGKYEIDEKDNSVSFRQKFGKNRYRKERIPFHVVSSSIKSLY
                     GLDYYLENSSKEGDYLIIDEPELSLHPENQVKLAKVITDIVESKIKLITSTHSDLFVR
                     ELTNIVIENKLNSKNNKKNKLSDKNIGIYNFVGNNDINELEKI"
BASE COUNT         7343 a         3125 c         4009 g         5633 t
ORIGIN      
        1 aacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ctagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccaaaga
      121 aagaccaacg gtcaaccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gagcgaatcg aaaataaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacag
      241 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgataa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgccacgatc cgaaaatacc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaaatc caaccattta ttttttgaaa
      541 ggctatcgaa aagttgcttt agaactgaat gaaaacgggg gatttgagga atcaaaaaaa
      601 taccaagcca cgtcggaaga ggaaaaagca gctgttcgtg aattacttga agaggctgca
      661 ccaactgatt ctctgaagga gaaagaaaaa gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggggaga tcgtaacatt agagttgatc
      781 gatcaactta aaacgttgga taaccaatac gctgcagaat caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgttacga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgag
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcatgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaaggat tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatt gaagtgatca aaaaacctta ctacacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacaa ttgaagcctg aagactatcc gaaaatcttc
     1501 ccgaaccgcg tctttgattt tcaattggat aaaacagggc agacagaact tcaagttggg
     1561 attcaagcgg tagccaaaaa tattggaatt caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtacgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gtcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tgtagaaacg gcagaacaaa agaatattgt tcctgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtgat tttaaaagga
     2221 aaaagttatg gaaaagagat tcaaacggcg ttaccttcag aagaattttc gctaggaaat
     2281 ccattccgat tttcacttga aacagtgtca gaagtggcca aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgc gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt ttgaaaatga ccgaaaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagacta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac tctaagcaac aatttaaagc agccacagat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtagaaa atcttcattt tgagacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gccgaactaa agccaaaaca tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaatg agacagtagg ggcgagttat
     3001 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagttaaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtttcgat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagttgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aagtcattta tgaagaagtc gcaaaagaag aaaagatcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaatt ggtttccgct
     3541 cacaaagtaa atgcgtcttt tggtcagcag ctttctgtta acgattcaat agaagctatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aagcttgtga acgctcgaat
     3661 ggtacgtacg attttgatcg tttttatcaa ggaaaagggg atattaatgc tcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa agcatgaaca tgaatttgta
     3781 cctgaagtga aaaaggattg gaacgatcaa ttgaagcatg agatccagca acaagaaatt
     3841 agaaagcaaa aacgtgcgat gttgtttcaa caaggcagac aacaagaaag ggagtgagat
     3901 cgtcacgatg aaaactgtca ttttagcaga aaaaccgtca caagcgaagg cttatgccga
     3961 tagcttttct aaagccaccc gaaaagacgg gtattttgag atccaagatc gattgtttcc
     4021 tggtgaaact gtgattacgt atggttttgg tcatttagtc gagttagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agaacattta cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaac gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgc gaaggggaac tgatcgcttg
     4261 gacgatcatt caacaagcag gcgccgatca tggaaagata tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcaggg attccagcag ttacgagatg cagaagagac
     4381 ctacccaaaa tttgaagaag cacaagcacg ccagatcgcc gattggttga tcggcatgaa
     4441 cggtagccta ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gtttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcaa ttacaggaaa agattagaaa
     4561 cttccaaaaa gaaccatatt ttgaaggaaa agcccaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt
     4681 aaaagaaaag ggggttcaat tgggaaaaca gccaggtacg atccaccaag ttgaaaccga
     4741 gaaaaaaagt gcggcaagtc cacgtctttt ttctctatcc agccttcaat ctaaaatgaa
     4801 tcagctcatg aaagctagtg ccaaagatac tttggaagcc atgcaagggt tgtatgaagg
     4861 aaaatatcta agctatccgc gaacggatac cccttatatt acggaagggg aatatgcgta
     4921 tctgttggat cacttagacg aatacaagca ttttttaaaa gccgaagcga ttccgactcc
     4981 tattcataca ccgaatagtc ggtatgtgaa taataagaaa gtccaggaac attacgcgat
     5041 cattcctaca aaaacggtta tgacagcagc ggcttttgaa caactaagtc cactgcagca
     5101 ggctatttat gaacaagtgt taaaaacaac ggtggccatg tttgcggaaa agtataccta
     5161 tgaagaaaca acgatcttga cgcaagttca gcagcttcag ctaaagacag tcggaaaagt
     5221 accagtagat ctaggttgga aaaagctgtt tggcaaagag agctacagaa aagaaaagga
     5281 agaagaaccg ctacttccta aagtgacaaa aggagaagcg gtgacggtag atctacaagt
     5341 ccttgaaaaa gaaacgaagc caccacaacc ttataccgaa ggtacgttga tcacagcgat
     5401 gaaaacagct ggaaaaaccg tggatagcga agaagcacaa tccatattga aagaagtaga
     5461 aggaatcggt acagaagcaa cacgagcaaa tattattgaa actttgaaac aaaaggaata
     5521 tatcaaggta gaaaaaaata agcttgtggt gacaaataaa gggattttac tttgccaagc
     5581 cgtggaaaaa gagccgctgc taacaagtgc cgaaatgacc gccaaatggg aaagttactt
     5641 attaaagatt ggtgagcaga aaggaacaca agctactttc ttagctaata tccaaaaatt
     5701 tgtctctcac ttgttggaag tcgtaccagg acaaatccaa tccactgatt ttggatcgac
     5761 gttacaagag gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt tagggatttg
     5821 tccaaaatgc caggaacaag aagttttact ctatcataag gcggccgcat gtacgtctga
     5881 agcttgtgat tttagattat ggacaacgat tgccaaaaag aaactcacag cgacacagct
     5941 gaaggaaatc attcaaaatg gccgaacaag ccagccagtg aaaggattaa aaggtcaaaa
     6001 gggttcgttt gaagcaacga ttgttctaaa agaagacttc acaacgagct ttgaattttc
     6061 tgaaaagaaa aaaacgaact ataagaaaag aacacgccgc actacaaaat aatggagggg
     6121 ttatcttatg cgaaatacaa cacacaaaca acaagaaatc gaaaatcaaa aagcaaaaat
     6181 tcgttcagaa caagagaaat tacgtgccag taaaagaaga cttcagcaat taaagaacca
     6241 gcaacggatt caaaaagaaa ttgaattaga tcgataaaat cttcctggct attgtatacg
     6301 cataaactta tgttatacta tatttataag tttatacgta tacaaaggag ggagaaaatg
     6361 acaaaagcaa tggtttacaa gctgctagaa gaatttaacg gaactttggc tttgaaggat
     6421 gccaaaaaag cgggcatttc tcctgtaacg atcaaacgta tggtagatcg tggagagctg
     6481 gatcgtgagt atccaggttt tttcacctta cctggtcaat ttcctgatga attgtttatg
     6541 gcacaaaaga aatatgagcg cggcattatt tcgcatatca ccgcgttgga tttatatgat
     6601 ttaacggata tgatccctag acagatcgat ctaaccgtac cttacggtta tcacgtttct
     6661 gaaaaggggt tgaaagaatt cgcagttgag ttacactatt caaaagcgga atggtatgaa
     6721 ttaggtaaga tcgaaataaa gagtcgttat gggaatcctg ttatagctta tgatccagaa
     6781 agaaccttgt gtgacatttg gaatccctgg tacaacgttg aggatgaaat caaagtaaaa
     6841 gcaatcaaaa actacatgga gtcggacaga aaaaatctta gaaaattgaa tgagtatcga
     6901 cgaattttgc ctacagacaa aacgatacgc tcttatatta tggctttaaa ttaaaggaga
     6961 agacacatga cacctacgca attaaaagca aaagtgaaga atatttccag agaaaaagaa
     7021 gttgatccgc agcttgtttt aagacatttt atgatggaaa aattcttgga aaaaatctca
     7081 gagtctcctt atcgtgaaaa ttttgtgtta aaaggcggct tcttaattgg ctcaaagtat
     7141 ggcattgaaa atcgaacaac aaaagacatt gatacgactt tacgggaaat gaaagtaacc
     7201 aaagaaacat tgacaacggt tctaaacgat attttttcaa cgccgacgaa agaaggcatt
     7261 caatttgaga ttcaaggaat gaaagaaaca agggaagctg attattatcc agggtttagc
     7321 ttacgtgtac tggcacattt ggaaaacatg cggccagatt ttaaggtaga cgtgacgaca
     7381 ggtgactcga tctatcctgc aacgatcact cacagtcata aattgatgtt tgaaaatcga
     7441 acgattcctt tggaatctta tcctacagaa caaattattg ccgaaaaatt aagtgcaacc
     7501 tttgattttt taacagataa ttcgagagga aaagattttt atgatctcta tactattcct
     7561 aaaatggaaa agatcgatga gaagaccttg aaagatagtg tgagaaatac atttacacgt
     7621 cgaggaaaaa ctgatccgtt aaagatttat tacgaaaagg atatggatgt actgaaaaat
     7681 agtcatgagc taaaagatac ctggaagaaa tatcaaaaaa gcaatcctta tgctgcgtcg
     7741 atcacgtacg aagagacaat ggattcgatt agtaatttga tggaaaaagt gattcacgtc
     7801 gaagaaaaag agcgaaaaat agcttatcaa agacaccaac agatgaacag agaaatggag
     7861 agataaaaat gatcaataat gtaactttag taggacggtt aaccaaagat ccagatttac
     7921 gttataccgc aagtggcaca gcagtggcta cttttaccct agccgttaat cgtaatttca
     7981 cgaatcaaaa cggcaatcga gaagctgatt ttatcaattg cgtgatttgg cgcaaaccag
     8041 cagaaacgat ggcgacatta gctaaaaaag gcatcttgat cggcgttgtc ggacggattc
     8101 aaacacggac ttatgacaac cagcaaggac aacgtgtgta tgtgacagaa gttgtagctg
     8161 ataacttcca actattagaa agcaaagcag cgacagagag ccgtgcgcac gctgatcaat
     8221 cgtccacgag tccaagcacc accacttttg aacaacggga tacagcgacg cctaacaaca
     8281 atggattgaa cgcttcacag aatccatttg gtggacaatc aatcgatatt agcgatgatg
     8341 atctaccgtt ttaagtagca ttttctattg gttgcatttg ctacggttta tgttataatt
     8401 tagtcaacaa aaagaatttt caatctataa ccaaaaacca ctagctggcg ggctagtggt
     8461 tttcttttac gtttctatta taagaattaa cgagtaggct tactgtcttt cttggcaaat
     8521 ttacaatcaa gccatttgca gatatagtgc gcggctactt ctgccatgat tgcaaaaaca
     8581 aaatcaaaaa gaattttcaa acctataacc tcctcccctt gccagattag gccggggtag
     8641 cagacagcaa gactattata gcataattga aatatccaaa tgattggatt tttgctataa
     8701 ttttagcgag gtgattgtga tgaacaaata tgttggtttg cttgataaaa tccgagtaat
     8761 aaaaacgcaa ccgttattgg ttcgctttac tttacaaacg attcatgaat cgattaattg
     8821 tgtagttgca gatattgaaa ttatcgataa attgctaatt atggatgatg gaaaatacaa
     8881 tattgccgtc acaggtcact tcaataaacg aaatcagctg gttattgcta gcatgtatgt
     8941 tagaaatccc gatcatttta cacgatcgat gggcatataa ataaaagaag agttgtagtc
     9001 ctaaatgagg attgcagctc tttttgtttt acatagaaac gggaacagga aaaggcctgt
     9061 ttaggaaggc acaagcgcct tctcaattaa aaaacctttc tctcactcca accgccaccg
     9121 ccgttcgaga agaaaggaag tgaacgctgc acgttcacta agatatggtg tcttgcaaag
     9181 caagacaaaa taggaaaagc ttcctctcac caaaaggttc gttcctgttc gctatctctt
     9241 tattgatttg ccagaccggc aacaagttgc cttttatcac caaaaagcga caaggacaag
     9301 ggtaaatggc acgctgcgcg cccttgtcct tgtcgctttt cgttgattca ttctggccaa
     9361 caaatcaata aagaaagaac taacgaaagg aagtaaaacc tttcctaaga ggaggcagaa
     9421 tcaatcatgc gaaagaaatt tgattattgg ggtgttccct tcagcgaatt attcccaaac
     9481 tatcatgcac cacatactgt cgaatgcgat tgtggagaaa gagcgaaatg tatcaaaagc
     9541 tatcgtttgt atcaatgtcc aacatgcggg aaaaaatata ccttgtctta tggcgattac
     9601 attttgattg atgagaaagg aaagaaacgc tgatgaaaac acaacacaaa ttattgaatg
     9661 taaaagaagt catttctgaa aaagtagcag aagctttact tattccgcgg tactggacga
     9721 tagaaacccc tttagtagag gacgcaccga ctatgctatt tgaagaccta gtgaagattg
     9781 caggatatga actgcattat caagaaaact tggaaactta tgaaaacgaa tattggttaa
     9841 ccaaattaga gaactaaaaa ggagaaaaaa tatggctcag aattactatg acgaatttgt
     9901 caaattacct ttggataaaa tggcacaaaa aatggaggat atgacgttcc tataccatga
     9961 aacacgtgta cccaaaaaac attacaagga aaaattgtca gtcgcagtgg aagaaatgat
    10021 tgaatcaggt gtcgaaatga atctcattgc gacgtactat cgaacattgg aagaactgaa
    10081 aaaacaaaat ggaaaatggt ttttccaagc cttactatgc ttggaagcag gcgtcaaacc
    10141 aagcacaatc aaaccttctg aataccaagc tttagaacta acgtatggaa aatttatcga
    10201 aacgaaaaaa gccaagactg tttcaagtga atggttggac tactttgaaa atgtcaacaa
    10261 gtatggcgca tgttacatca tgaaaaagga ggacaacgaa aatgagtaat gtggtttatc
    10321 aactaacagt ggaagatgtt tctcactttt taacaaacaa agaacatttg aatgaagtag
    10381 tcagacggat tcaaaaagac tttagttgta tggaagaaat gaagtacttt attcaaagag
    10441 tggaagaaca gttgtatatg gaaagcgaaa cgagtcaaac gaaggtcaca gtcagccata
    10501 atcatttaga cgtgtataaa cgtcgtcctg aaacggtaaa aaaaggaaca gagcttctac
    10561 gactagaaac agaaaaatac atcatgaatg tgtacctaga aaggagtgag ctggtcgaaa
    10621 actttgtcag ctacacgatt tgtaggaaac actattactc agaatcagaa tattattcaa
    10681 tttttgagag catggaatta atggaagaag aaatggaaga gctaaacagc aaaaatgcga
    10741 cggtagacga ttattacctt gtatttacta gatttctgac gtgtgggacg gaatctatcg
    10801 tgttaatggg aatggattat gaactcgctg tacccgaagc atttttgagg gaatatgtag
    10861 aagaagtaga taacccaggc gacgactggt acacgacttt aactgatttt ttagatacct
    10921 acacttacga cgatacagaa gaaatccaaa atgagcttga ctgtaaacga atgccatatc
    10981 aacgatttga aaggaaaaaa ggtcaataac catttatacc gaaaagtgag aatgtgtgaa
    11041 ataagaacaa aaaaccttga aattaaggga atggttctta tttcgcacca atcgctccaa
    11101 ccgcccccgc cgctcaggtg gtgcgaaata agcgggcaac gaccgataac ctaagagaaa
    11161 gaaggaaatc catatgaata aaatcattcc tctgttgttc gtactgtttg ttggcgagat
    11221 acttatgcgt catgctgcag ctgtacattt aaacttcgta gtattcttag gaatactggt
    11281 attttgtttg atcttttggc gatttgtatt agcccttgtg agtctactat tcaagatgat
    11341 tgcagccata tttttcatag ggttatttcg atgaaaggag agagtactaa tgccaattgc
    11401 aataggaaat aaacgtttac ccgtgacgtt ggatgaaaag agacaaaaag aattgcagga
    11461 actcaaacag aagtacggca aaagtgaatc caggattatg tgtattgcgt tagatttatt
    11521 gattgcacaa gaaaaagcag ggtttgatgt accagcactc aaaaagtgac gtcacttttc
    11581 atttgaagaa acaaaagtga tagcacttta cgcacgtgtc gatacgtgtt atgattattt
    11641 tgtaaggagg tatagtgcac aatgccactt acgggaaaag aaatgcttaa gttattgaaa
    11701 aaaatggctg ggttgagcga aggcaagagg gatctcatca tcatctttat aaggatggtg
    11761 ttagaatcac tgttcctgtt catgccaatc aagatcttgg ccgaggactt gagcaaaaaa
    11821 ttttaaagga tgcgggattg aaataatctc gcattcgatt aaagaaagga ttgactgaaa
    11881 tgttaagtga gcctttagca aaaacctatc ctgctatttt tagcccagaa gaaggtggtg
    11941 ggtactttat tgaatttcct gatgttcaag gtgcttacac aggaattaac gaagacgata
    12001 tatcttatgg aattgctatg gcacaagagg tgttaggtat ggttttagca gattatattg
    12061 agcatgaaga tctacttcca gaaccaacac caatcaataa gatttctgtt gaagatgatt
    12121 cttttactac tttaattcgt gtagatgttg ccaaatattt gaaagacaca gaacttgtca
    12181 aaaaaacctt aacaattcct caatgggcgg ataagctagg caaaagggct ggcataaatt
    12241 tttcagtcct tctaacagaa tcaattgcag ataaagcaga taaccttctt cgttctggaa
    12301 gaaataatta gaactcaaaa gagtcagaaa cttaaaaatc tttggcttct ttttttacta
    12361 caaagatggc ttctttaaat tatttgcctg attgatttaa aaaaattgcg caaagactac
    12421 gagctaatta agttgctcgt ggtctttctt tatgaaaact ttttagatct agataaaaac
    12481 attgagacac tttgaaaaat ttttttaata gtaatgggcg cttctatgtc aagctgattc
    12541 ttctttcggt aaattaattt tacttagaat atcactatct tgataaaaaa tggtgtattc
    12601 acaaattctg gcataaaaaa tacctacttt ctagttaagg ttcctgaata agtcgcaatc
    12661 caattatttc cagctgattt ataacttttt aaatataatt ttccataata ccaataacca
    12721 ttactttttt ctgtccaatt tcgttattga gaagatctag aaggccagcc aactttatca
    12781 aaaatcatca ttcctgtcac tcgtatcgtt ttagaagctg ctcgagtaag taatggagaa
    12841 actgttaaat aattttaagt aacagttaag tcgttagttg ggttatcaat ggctattccg
    12901 tccttatctg aacctcgaaa cccaatcata gaaaaagttt tttgaacaag ttttaacaga
    12961 taaatcaagt tatatactag aaaattcgta aaatatattt ttaatgtcat aatcgtttga
    13021 tttttctgga ttgacattta tttataaaaa atagtgccat taaaaaagaa aagtgacaca
    13081 ccaataaaag tcatatcctg tgacattata tggcattatc caaatagaat aaccggttga
    13141 catgtaagcg gattcctttt ataattttag gagtataagt ccttttctaa aaatattaac
    13201 cggttatcat gaaaggggat acaaatatat cgttggagga taaaaatatg tcaaaggtta
    13261 acgtatttaa aaatcaatcc tatttacaaa gttcagctac attattacta ttttttgctt
    13321 cttggggtgt ttggtggtca ttttttcaac tttggctaac atctgaatca aatggtttag
    13381 ggttatctgg cagtgctgta ggaacagtat tctcggcaaa ttcgttagtt accttaattt
    13441 tgatgtttat ttatggaaca ttacaagata aattgtatat taaacgaaat ttattaattt
    13501 ttgcttctgt attagcgaca cttgttggac cattttttat atggatatac gggccattgc
    13561 tagataacaa ttttaattta ggcattatta tgggagcact atttttgtca gctggatatt
    13621 tagcttctgt aggagttttt gaagctgtgt cagaaaggtt tagtcgttta tttggctttg
    13681 aatatggaca agcaagggcg tggggatcat ttggttatgc cttggtagcg cttttggcag
    13741 gatttttatt tgtaaaaaat cctcatttaa acttttgggc gggatctttc tttggttctt
    13801 tactattgtt aaatttatta ttttggaacc ctaaagttga acgggaagca aatcaaaatt
    13861 ttaatcaaga acaagctgaa tcaaatagta ttccttcttt aaaagaaatg tttgatctaa
    13921 tgaaactgcc tcaattatgg acgataatca tctttattgt ttttacatgg acattttata
    13981 cggtattcga tcaacaaatg tttccgggat tttatactgg tttgttttca acatcagcta
    14041 atggtgaaaa aatatatggg acattgaatg ctattcaagt attttgtgaa gcgttaatga
    14101 tgggaattgt tccaatcatt atgagaaaat taggggttcg aaatactttg ttattagctg
    14161 taaccattat gtgtgtacga attggattgt gcgggtttgc ctcgacacca ttatctgttt
    14221 catgcataaa aatgttgcat gctttagaag taccattatt tacattacca atgtttcgct
    14281 attttacact tcattttgat acaaagctat cagcaaccct ctatatgata ggatttcaga
    14341 tagctgctca aattgggcaa gtgattttat caacaccatt gggaatatta agagacaacg
    14401 ttggctatca accaacattt aaaattattt ctcttattgt attactagca ggcatatatg
    14461 cattctttat tcttaaacaa gatgatagag atgttcaagg ggatccattt attcgaggat
    14521 aatggaaacc aacagaataa aaaacaattt tatctagaaa gtgagcgaat atattatgac
    14581 aaaaagagct atagaattaa caaatacacg ttatcgtttg ggatatcatg tgtctgcacc
    14641 atcaggatgg attaatgatc caaatggatt ttgctatttt gatgggtatt atcatgtgtt
    14701 ttatcaacat catccatata gtgctgaatg gggaccaatg cactgggcac atgctagaag
    14761 taaggattta gttcattggg aatctttacc acttgctttg actcctgaag atcaagaaga
    14821 tgaaggtggt tgtttttcag gaagcgctat tgaaaaaaat ggtgtgttgt atctgttcta
    14881 tacaggacat cactattatg gagacaatga tccagatcat ttttggcaaa atcaaaacat
    14941 ggcgtatagt acggatggca ttcactttac taaatatgaa aaaaatcctg taatagcaaa
    15001 agcaccagaa gataacacac atcatttcag agatccaaaa gtatggaaac acgatgaaaa
    15061 atattacatg gttttgggta gtcaagaaaa agatggatta ggtcgtgcta ttgtttatca
    15121 gtcgttagat ttattaaatt gggattacat tggaccaatt tctaaagcga aggaagtact
    15181 tacagaagga tttatgtggg aatgcccaga cttttttgaa ttaaatggaa aatctattct
    15241 tttattatcc cctcagggaa ttgaagcaaa tggaaaagat tatttaaatc tatatgaaac
    15301 aggctacttt atcggtgatt atgattatga gacagcaaaa ttcattagag gaaattttta
    15361 tgaattagat aagggacacg atttctatgc aacacaaaca actttatctc cagatggtag
    15421 acgtattgtt atggcttgga tggatatgtg ggaaagccct atgcctgaaa aagtagacgg
    15481 atgggcagga gcattaacgc ttcctcgaga attagagtta attggagatc atttgtatat
    15541 gaaacctgtg agagaattag aacaacttcg agttggcagc ggcgttgaaa caaaagttaa
    15601 tttggcagga gaaataataa tcgctcaaga tgcttcattc aaagaagctc taattgatgt
    15661 actattgact aaatcaaaaa atcaagaaat caaattttca ttaaaaacaa aagatgaaga
    15721 gttaattaat attttttatt ctaaggataa aaatgaatgt accttaactc gtaacggcaa
    15781 aaatgatcca aggtatggaa ccattcagat gtgtgataga ttatcacttc gagtgtttat
    15841 cgataaaagt tcggcagaga tttttattaa tgatggcgag cttgttttta cagagcgtta
    15901 ttatacagaa aatcaaccaa gtatgcattt gagtgttagt gaagaagctt ttgtctcgag
    15961 tacaatttat catctaaaaa aagatgcaat ttcttacgag taaggaggtt ttatgatgtt
    16021 aattggtagc atagaagcag gtgggactaa atttgtatgt gccgtgggag acgaggatta
    16081 ccgagtacaa gaccaaataa tttgtccaac gaccaccccc agggaaacat tgaaaaaaac
    16141 tgttgactat tttaaacagt ttgatataaa tgcattaggt attgcatcat ttggtccaat
    16201 agaaattcga aaaaattcac caaaatcagg atatattacg tctacaccaa aggtgggatg
    16261 gcgaaatgtt aatgtgttag gttatttaca agatgagctg aatgttccaa tgtcttggac
    16321 aacggatgtg aacggatcag cttatggaga gtacatcatg tccactttat cgaatgaaaa
    16381 aataaattca ttagtttatt acaccgttgg tactggcgtt ggtggtggag ctgttgttga
    16441 cggaaaacta ttaggatcgt ttggtcattc agaaatgggc catacgtttg taaaacgcca
    16501 tcctgatgat attgatttta aaggaatttg tccttatcat ggtgattgtt tagaaggctt
    16561 agttgcggga ccaacgtttg aagcacgttt tggaaaaaag ggtgaagacg tttctttaac
    16621 ggatcctatt tgggatgtaa tggcctatta tctagcacaa gctgtgattc aaacaacatt
    16681 gattatacga ccggataaaa tagtatttgg tggaagcgta gtaaatgaaa aattgttaga
    16741 aaaaattcgt gttaatgtta atgaattatt ggatgattac gtggaaatac caccgttaag
    16801 taaatatatt acgcgtcctt taattaaaaa taatggatcg gcaacactag gaaactttgc
    16861 tttgggcttg aatcaattac aagaataaaa catagaaaaa atatcgatta tcaggtatag
    16921 tagttctata gagttctttt tttcgatagt taattattgc attatttagg agagacaaga
    16981 gaatgcgccc aaaattagaa gatgttgcca aaagagcaaa tgtatcaaaa acaactgttt
    17041 cacgtgtatt aaacaatcgt ggctatttaa gtcaaaaaac gattgataat gtttataagg
    17101 ctatagaaga actcaattat caaccgaacg ttgtagcgcg tcaattattt caaaagaaaa
    17161 ctaacattgt cgggttacta tttcctacag tagccaatcc tttttttagt gaactcgttg
    17221 aggcattgga aaaaaagtta tatgaaattg gttataaggt attaataggc aattcgatga
    17281 ataataaaga aaaggaaaca aattatttaa atcagttatt gtctgatcaa gtagatggtt
    17341 taattgttgg tacacataac caagggattc aggaatataa atatcagaat ttaccgattg
    17401 ttgcaattga tcgagtgatg aatgaagata ttccagttgt tgaatccgat aactacaatg
    17461 gtggaaaact tgcaaccaag ttattaattg cacaaggtgc aaaaaatatt attcatacaa
    17521 acggtccaat tgacttacag actccggcta atcgtcggag actagcctat gaagatacta
    17581 tgaaagcata tcagttaatt ccacgaactg tgacattaga tttcaatatt tcttatgtaa
    17641 agaaaaaaca gatttttttc caaatgtttg aggattatcc taaaatagat ggtatttttg
    17701 cttcaaatga catcgatgcg gccctaatat tacaagtagc aaaagaaaag gggttaaatg
    17761 taccagctga tttattagta gtcggatatg atggtacttt aatgactcgg agtatattac
    17821 cagatctgac aacagtaata caaccaatta atgatatagc agacacagct gttgctattt
    17881 tgatgaaacg aataaataaa gaagaaacaa aaaaggaata tattttacct gtcacattat
    17941 ggatggggaa gacatcagta aagaaaaagg acaattaaat ttataaagac tatcaatgat
    18001 aatttcatta ttaattcgtg atataaaaag ttgttaaatc tcttataata gactatgata
    18061 caaaatcatg cttttaatct gtgtagatgt tgccaaatat ttgaaagaca tagaacttgt
    18121 cagaaaaacc ttaacaattc ctcaatgggc ggataagcta ggcaaaaggg ctggcataaa
    18181 cttttcagtc cttctaacag aatcaattgc agataaagca gataacattc ttctttctgg
    18241 aagaaataat tagaactcaa aagggtcaga aacttaaaag tttttgatcc tttattttca
    18301 agtctgattt gacagatttt cggcagctca tttatttatg gtcagaaata gttgctgaaa
    18361 ttttaatcta ttagcaaata gtccagaacg tatgaagagg attcggatat catttgtcat
    18421 taacaccata aatatttata aaattcgtgt tcacctacaa ttaaatttaa tatataatat
    18481 tcgttgtaag ggcttataat tttttgttaa tgaacaagat atgtaattga tagtgtcaat
    18541 agctattcaa aaatgtaggc ttttggccca taaataacaa ggttacttat atgataactt
    18601 tttttcttta taatctttaa tccgatagag ctttccagta gtttgaataa tcttagcgtg
    18661 gtgcactaat cgattgatta gtgcatttgt gttcagtcca ttgagacata atagtggata
    18721 gttttactga gaaaattgat aaaaaataga gatataattc aaatacgtat ataatgaaac
    18781 taaagataaa tacggaggaa aatatatgat tataaaaaac ttaggaaatg ttaaatcaat
    18841 agatatacac gttaaagatt tgctgatttt tactgggaaa aataatacgg gaaaaacata
    18901 taccagctat attttatatg gaattttatc agaactcttt ggagcaagat ttttcaatta
    18961 tttttctcat acagaaattg aaccacattt acaaaatagt ttaaatattt tattagttag
    19021 caaagactct ataaggaaaa aatttaataa tgatgcaaaa aaatttttag aagaaaattt
    19081 aaaaaaaatt gcagttcgta actttaaagt aagtgaaaat aacttcaaaa atctttcttt
    19141 tactatttca gattcagaaa ttagtgagct cactggtgat atctttaatt atgagatcaa
    19201 aagatacagt ataagtggca ttgttataac aattcaaaat gtaggagaga aattaaaaat
    19261 tagctttgag ccaactgatt catttcagta tcttagcaaa gatattgaaa ttgaagatat
    19321 ttataaaatt ttagctcaag agctatctaa aaatgttatt agtttgccaa aggtttttta
    19381 ttttccagcc gaaagaactg gtataaatgt ctttaagaac gaactaaatg aaagtcgctt
    19441 aaaaacatat gataccttgc tgaatactgt gcaatttact aatttaacta accaaaaaaa
    19501 gaaagctgcc ctcaagtacg atctgttaaa aaagaatatt gagctgttaa tggagacaac
    19561 ggataatagt tcttatccaa agccaatatc ggattatatt aattatctga ataacatgaa
    19621 aaatagatat gaacagaaaa atgtcaataa tatagcatca tatattagag atgagattat
    19681 tatgggtaaa tatgaaattg acgaaaaaga taattctgta agttttagac aaaagtttgg
    19741 taaaaataga tatagaaaag aacgaattcc ttttcacgtt gtgtcttctt cgattaaatc
    19801 tctatatgga ttagattatt acttagaaaa ttcaagtaaa gaaggagact atcttattat
    19861 tgatgagcca gaattgagtt tacatccaga aaatcaggtt aagttagcaa aagtaattac
    19921 agatatagtt gagagtaaaa ttaagctaat tacttctacc catagtgatt tatttgtacg
    19981 tgagcttaca aatattgtta ttgaaaataa attaaatagt aagaataata aaaaaaataa
    20041 attgtctgat aaaaatatag gcatatataa ttttgtagga aataatgata taaatgaatt
    20101 agaaaaaatt
//
LOCUS       DAIGOK010000047        19663 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.047, whole genome shotgun sequence.
ACCESSION   DAIGOK010000047 DAIGOK010000000
VERSION     DAIGOK010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.047"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            22..302
                     /locus_tag="NOA01_001333"
                     /pseudo
     CDS             22..302
                     /locus_tag="NOA01_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(380..913)
                     /locus_tag="NOA01_001334"
     CDS             complement(380..913)
                     /locus_tag="NOA01_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCI0632536.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(1007..2185)
                     /gene="mutY"
                     /locus_tag="NOA01_001335"
     CDS             complement(1007..2185)
                     /gene="mutY"
                     /locus_tag="NOA01_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCI0632537.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2178..2975)
                     /gene="recX"
                     /locus_tag="NOA01_001336"
     CDS             complement(2178..2975)
                     /gene="recX"
                     /locus_tag="NOA01_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCI0632538.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNRGKSQKELLRKIQQTLMTKGFSQEVIQQAMADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            3082..4464
                     /gene="rlmD"
                     /locus_tag="NOA01_001337"
     CDS             3082..4464
                     /gene="rlmD"
                     /locus_tag="NOA01_001337"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCI0632539.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMICEINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4638..5216)
                     /locus_tag="NOA01_001338"
     CDS             complement(4638..5216)
                     /locus_tag="NOA01_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HCI0632540.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5538..6008
                     /locus_tag="NOA01_001339"
     CDS             5538..6008
                     /locus_tag="NOA01_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HCI0632541.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(6116..7342)
                     /locus_tag="NOA01_001340"
     CDS             complement(6116..7342)
                     /locus_tag="NOA01_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCI0632542.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(8096..8296)
                     /locus_tag="NOA01_001341"
     CDS             complement(8096..8296)
                     /locus_tag="NOA01_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCI0632543.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8430..9038)
                     /locus_tag="NOA01_001342"
     CDS             complement(8430..9038)
                     /locus_tag="NOA01_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCI0632544.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(9123..9593)
                     /locus_tag="NOA01_001343"
     CDS             complement(9123..9593)
                     /locus_tag="NOA01_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCI0632545.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9641..10555)
                     /locus_tag="NOA01_001344"
     CDS             complement(9641..10555)
                     /locus_tag="NOA01_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCI0632546.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10701..11504
                     /locus_tag="NOA01_001345"
     CDS             10701..11504
                     /locus_tag="NOA01_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HCI0632547.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11682..12629
                     /locus_tag="NOA01_001346"
     CDS             11682..12629
                     /locus_tag="NOA01_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCI0632548.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12697..12990)
                     /locus_tag="NOA01_001347"
     CDS             complement(12697..12990)
                     /locus_tag="NOA01_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HCI0632549.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(13018..13683)
                     /locus_tag="NOA01_001348"
     CDS             complement(13018..13683)
                     /locus_tag="NOA01_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HCI0632550.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13823..14455)
                     /locus_tag="NOA01_001349"
     CDS             complement(13823..14455)
                     /locus_tag="NOA01_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCI0632551.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14462..15529)
                     /locus_tag="NOA01_001350"
     CDS             complement(14462..15529)
                     /locus_tag="NOA01_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCI0632552.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            complement(15526..16257)
                     /locus_tag="NOA01_001351"
     CDS             complement(15526..16257)
                     /locus_tag="NOA01_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HCI0632553.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(16425..16904)
                     /gene="greA"
                     /locus_tag="NOA01_001352"
     CDS             complement(16425..16904)
                     /gene="greA"
                     /locus_tag="NOA01_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCI0632554.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(17040..18215)
                     /gene="mltG"
                     /locus_tag="NOA01_001353"
     CDS             complement(17040..18215)
                     /gene="mltG"
                     /locus_tag="NOA01_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HCI0632555.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            18380..19540
                     /locus_tag="NOA01_001354"
     CDS             18380..19540
                     /locus_tag="NOA01_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HCI0632556.1"
                     /translation="MSKGKHRKKQKISISFLVILLLSVSFFSTGIIIGGQLTKIPHSQ
                     TYKAEPTQLTSNLTPDEQRLASKIDQLLQKSDYIGSIYVRKNNRVLLEKGYGYANLRV
                     KESNSPSIYYQIGSIQKAMTALLILKQIQAGKLTFDTKLAEFYPQIPGSQQITIKNML
                     YMRSGLHRTASPDKPMSDSEIIQFALHHLKLTDYDTYHYEPLNYTLLTGILIKLTNQP
                     YETLLKKEIIRPLHLEHTDFYDNVKNSPQHAVSYQMSATDDYSKALIEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGGIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVVAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVHLSN"
BASE COUNT         5903 a         3984 c         3162 g         6614 t
ORIGIN      
        1 cgaaaggaag gtggctagac catgaaagtt gtacaaacaa aagatacaat gagcaatatt
       61 tacttagatg ctgtacggat caggcatcaa gtttttgtag tcgaacaggg tatgcctttg
      121 tctcgagaaa tagacaaaga tgaagcccat tgtatccact tcgtcctgta ctcagataaa
      181 aaagaaccgc aaggaactgt acgtcttttg cctttagaaa acggaaaaat gaaattacag
      241 cggatggcga tccttttgaa gatgctggca tgcctcatat ttatatgaaa aagactttat
      301 aaaaatttat ttcgaaaaaa gcaaaaaaca gcaagctttt gagaaaaatc tctcactggc
      361 ttactgttct tttttgtgtt tacttacgcg acaattgctt gtaacggtta taccaaatat
      421 caatatagcc ttccgagaac gggccatctc cattattgat ccagtcaacc agaatcttca
      481 cattttcttt taggatgaag tcaatatcgt tcggataatt catcattttg ctgtgcatct
      541 catattcatc gacatccaat agtcgtttct cgccgtcagg aaataccttg atatccaagt
      601 catagtcgat atatttcaag gcctcatcgt ccagcaaaaa aggtgaagct agattacaat
      661 aataggaaac ccccttttct ctgatcattg ctattacatt gaaccagtat tttttatgaa
      721 aataaacaat cgctggttcg cgcgttaccc atcggcgtcc atcagattca gtgactaatg
      781 tgtgatcatt cacgccgatc atagagtgtt cgcttgtttt taataccatg gtatctcgcc
      841 acgttcgatg caatttgccg tcgtgcttgt aactttggat cgtcacaaac tctccttctt
      901 ttggaactcc catagcaacc cttccttaag cagcattttg tgctttgtgt ttgggtgttc
      961 attacaccga catcataatt attatatcaa taaaaatgag ctttgcctag aaaggattcc
     1021 tattgtttcg atttttctct aactgatcta ccagtttcat ttggacctta ggaaaaacga
     1081 ccgaatcaaa tgcatcaggc ttcaaccact tcgaagtttt gttctcggta aattcttgtt
     1141 ctgctccctc ggttgcccgt ccgtaaaaaa ggagaacatg ccatttcaga tgactaaaaa
     1201 tatgagtaac ttctcctaaa tgtcgtgtct gccaaacaaa gagttcctgc ttttcaaatg
     1261 ggagattttg ctcttcttct gctactaggt catcaaacag atcgatttct ggctttgttt
     1321 cccattcttt tttcaatcgc tcatactcct cttgtgtcac ttccatcatt ggaaatgtcc
     1381 acatattcgc cagcaatttt tggctatctc tttcttcaaa ataatacgct ccagaatgat
     1441 tttgaagagc cgcactgata taataaacgt cttttggctt tgcttttttt gtttttacag
     1501 gaaaactcgt ttggattcct cgtttgttag ctaaacaaaa ggcttggatc ggacaagtct
     1561 cacactttgg agaagttggt gtacagatag ctgaacctag atccatcatt gcttggttga
     1621 attcacctgg atgcttttca tcaatgattt tccgcatcgc ttcatcaaag attttcctgc
     1681 tagaagcttt tgcgatgtct gcttcaatgc aaaacaatct gctgactact cgcatcacgt
     1741 tgccgtcaac cgcgggttct ggaagtccaa atgcgatact tgcaatcgcg cctgtcgtat
     1801 atggtcctat tcccttcaat gagctaattt cttcaggcgt ttgcggcatt tccccatcaa
     1861 attctgacat gatctgttta gcagcagctt ggatgttccg ggctcttgag taataaccaa
     1921 ggccctccca tgcttttaaa agcttttctt ctggagcatt tgctagctct tcaattgtag
     1981 gaaaccattc catgaaacga taaaaataat cgatcactgt gtctactctg gtttgctgaa
     2041 gcatgatctc agagatccag atacgataag gatcacgatt atagcgccaa ggcaggtttc
     2101 ttttttcttg ttcataccat tgaatgaact gatcttgaaa ttcttttgtt tctttatctg
     2161 tccatttttc ccattcttta ttcactttcc tcttcctttt ccgtaatgaa tcgctggatc
     2221 atatccaaat caaatccttt ttgatataag ctttgcttta cttttagatt tcttttttta
     2281 ggttcaaacc ttaaatttct tcgccatagt ttatctcctt gtttgactaa ataatcatat
     2341 tctatttctt gatctgcttc ttgcggaaga tcagccatcg cttgttggat cacttcttga
     2401 gaaaaacctt tcgtcatcaa tgtttgttgg atttttctca ataattcttt ttggcttttt
     2461 ccgcgatttt ttccaaacgc tttttcggct gtctgatgtg cattttgaac ttggtcttca
     2521 aatgtatatt ggctgagcgc ttgttcaatg atttcaggtt tcaaaccttt ttgctgcatc
     2581 tgctgcgcta atttctttgg tcctttgtca cttaagcgca tattcgtgcg cacataactt
     2641 tccccataga ccaaatcatc gacaaggtcc aattctttta agcgggctac gattttattt
     2701 cgatcttctt gaaggatttc cttttctttt aagtatgttc ggatttcttt ttccgatcgc
     2761 aactgataac ttaagtaatt cattgctagg tgaaaaccga aatcagctcc gctactcttc
     2821 ttgatctctt ggatagtttc ttcgtctaat tcccgtcctt ttaataatcg atagcgtact
     2881 aatacatctt cagatacttg taaagcttct ccttcagata aatctacgcg atagaaaggg
     2941 cctttgcctt tactgattct cgtaacggtt aacataaaca tcctcctcga acatatattc
     3001 tctatcatac cacagaaaat ccccgtaatc aggtattata tgttagaata aaccacagaa
     3061 aaaagaaaaa gggagatact tatggcagaa acaattgtca aaactggaca aacggttcct
     3121 ttgaaaatca agcgtttagg cataaatggt gaaggaatcg gttatttcaa acggttgatc
     3181 atatttgttc catatgcact accaaaagaa gaagtactag tgaaaatcac gaaagcaact
     3241 cctcgctatg cagaaggaga attaataaaa gtcaaaaaaa caagcaaaga tcgcgtagtg
     3301 gcaccatgtc ctgtttacta tgaatgcggt ggctgtcaat tacaacactt agcttatcaa
     3361 gctcaattgg attttaaaaa ggatctgctt ttgcaagcat tggaaaaatt caaaccagca
     3421 ggctttcgat cttatcaact tttgccgacg atcgggatgg atgagccttg gcattataga
     3481 aacaaagcac aatttcagtt acgaaaaaat aagcagactc aaaaaattga agctggttta
     3541 tacgaagcaa actctcatca gttagtccct ataacagact gcttggttca agaacctcga
     3601 acacaaaaag taatgaatac tgttgttcaa ctattgaata aatttgatgc acccatctat
     3661 gatgaacgga caaacagtgg aatcttccgc acaatcatgg ttagaatcgg tgttaaaact
     3721 ggggagttac aagttgtttt tattactcgc agtaaaaaat tcccgcaaaa aaacgcaatg
     3781 atctgtgaga tcaatgaacg actgcccgaa gttgtttcaa tcatgcaaaa tgtccaggca
     3841 caaaaaacgt ctctcgtgat gggggatgaa acgatccact tatggggaaa agaagccatt
     3901 gaagaacaaa tcaatgaagt gactttcgat ctttctcctc gtgccttctt tcaactcaat
     3961 cctcagcaga ccgaattgct ttatggcgaa ggaatcaaag cattagatgt ccagcctaat
     4021 gaaacgatcg tggatgctta ttgcggagtt gggacgatcg gcttaagtgt agcaaaacaa
     4081 gccaaagaag tacggggaat ggataccgtt cctcaagcaa tcgctgatgc aaaaaaaaat
     4141 gcggaaagat taggatatca caacactcga tatgaagtcg ggacagcaga gcaagttctg
     4201 cctaaatggt tgaatgaagg ttttcagcct gatggtatca tcgtagatcc accacgtacg
     4261 ggtttggata acgcattgat ccaatcatta ttgaaatacc ctccaaaaaa acttgtttat
     4321 atctcctgta atgtttctac acttgccaga gatctggtta atttaagtaa aaagttcaag
     4381 attgaatatt tacagtctgt cgacatgttt cctcagactg cacgttgtga agttgtcgtg
     4441 aagatgacga gactcgctga ctaaatgaaa gaggttgtga aaaagtctgc cctgtatcaa
     4501 gtaataagaa cagccaaaca aaaatagctc ttcatgtttt atgaataccg tacttgtgac
     4561 cgaagagtta acaggaggac actatttatt taaaaaaagg gttgtgacat agtttttgtc
     4621 acagcccttt ttgattatta ttggatattc acgtctatca cttgataaaa tgcatttgtc
     4681 gtatcgctga tattccatac tgctaaaatc acattatatc cttttcgatc ctgcggaagt
     4741 gtgatcgttt ggttgactgt ttttggtgga acagttgctt tgtcgtctat ttttgtgatc
     4801 aattcaaaat cactaaattt caaaggttga ttaggattcc aagttggttt ggtaatgtag
     4861 taatcccaag tacttgtccg atgttgtgct gttaaattcc aagtaatatt taatgttcct
     4921 ggattaacca tgtttttatg ccatctgtta gatgtctgtt catctaaagg ctcaaagccg
     4981 ctgactcctg cactagcaat ttttccatca atgaatgtat ttttggtagc ttcgatactt
     5041 tgcggttccc attgtgctct tccgacattt tgatttaagt ttcctgctgc tgatgttcct
     5101 aggtatgcac ggcttcctgg agaagtaata taaccatgcg caaaacctgg aatgctaaaa
     5161 ccgaacaacc ctaaactaac taacccaata aacaataacg tactaatctt tttcatgtag
     5221 aaacctcctt catttgataa ccacattgta aacgctttat tgtattaaca caaacattat
     5281 gcttttataa ttttatgttg aagtaaaaaa ggtgaaaaaa gtaagatgca gattataaca
     5341 gaagtagacg agaaaagtac aaatagaaaa catgagaacc aaatcatatg atctttttca
     5401 aaaacatcat tcatataaac aaatgttcaa ctttttaata acaacaatca tcaatttttt
     5461 tcttacttca caaactaaat acaggtcttg ctgtgagcct tcatggtaaa ctactattat
     5521 agaaaggagt tagtaaaatg aagatgataa aagatattcg tttttgggtt tgtgttatag
     5581 gaattgtcat attaggcttt ttatcagggt tgcttagcgg aaatcccgga gaatattact
     5641 attctttgca acttccacca tttgcaccac ctagctggat atttgggccg atgtggacct
     5701 tactgtatat attgatgggt atttctcttt atttgttact taatcacaac aacaaaaaac
     5761 agcgaaacaa tcttgtcggt ctttttgtga ttcagttcat ttttaatttt atatggtctg
     5821 ctctattttt taatttaaga aatattttca ttgcagcaat cgatattaca cttttagtta
     5881 ttttcttgtc agtgcttatg tatcagttgt ggctacatca tcgcctggca atgtggctca
     5941 tgattcctta ttatttatgg gtactattcg caactctatt gaattattca atttattttt
     6001 taaactaagc gctaaaaaga gacgtcctgt aatttaacac atagaaaaag tgccacctaa
     6061 gttagatttt ttgtctaatt taggtggcac tctttattaa ccaaactatt tttttctagt
     6121 acccacttgt tgttccatac tcattattct ggtcataacc ataaccattt tgttcctgtc
     6181 cagcgtaagg atcataactt tgagtatctt gacttggtgt atagtactga cttgtatctt
     6241 ctgtttgctg ataataggta ttcggatctt ggtactgatc atatgtgttt tctgaatagg
     6301 aagaataatc ggtgtaccct tcactattcg atccatcacc gtttgtcgaa tcgccatctt
     6361 ccgaatcatc aaagaacatc attgaagcat tctcattttt caaattaggt aatgtatcgc
     6421 ttgtttttat atttaattgt ttcttcagtt cattttggat cgaaagaaga tcattttttc
     6481 ctaaaatttg atagtagata tcattgatca tttgtccttc gccttgcaac tggtcttgtt
     6541 tgattgtttc aaacgcaggg gtgtagttcg tcgctacatc aagcatgtca tcccaatcta
     6601 agtccgtcgt cacattgttc tcaactgctt ttaaaatttt tctataattg cttacgccat
     6661 ctaaacttaa aactttattg acgattttcg tcactacttc ccgctgacga cgttgtcgac
     6721 cgatatcgcc ttctggatct tcgtgtcgca ttcgagaata ggctaaacct ttttctccat
     6781 tcaatgtttg ttcacctact ggtacatgga caccatccaa agtaaagtca attttattat
     6841 tcactttgat gccaccgacc gcattgatca aatcttccat gccatccaaa ttgatcgtta
     6901 cataatgatc aataggaata tcgagcagtt gctcaattga atccatggcc atttcgacac
     6961 caccaaatgc ataggcatga tttaatttat ctaccgttcc ataacctacg atattcgtat
     7021 aaatatctcg gtccaaactg atgatcgttg attttttttg tttcggatta actgttacta
     7081 ccatcatcgt atcagaacgt ccttgttccg ttcttcctaa tccaccggta tctaaaccta
     7141 atagcaaaac cgaaaaaggt tctttgtcat caatgcttac tctctcagaa cgtttggagg
     7201 atattcgatt agagttttta gaaattttgt ttactgtttg gctcgcttct cccataaatc
     7261 tgattccgta tgctgataat ccggcaatca gcaaaacaaa tacccctaac aaggttaata
     7321 gaactttttg ccatcgcttc ataatacacc tctgtttcac attttctaaa atattgtctc
     7381 atataactgt tcaatcagca aattcacttt ttaatcataa gaaaacttta aaaaacattt
     7441 ttgtgtaacg attactattt tttaaaattt ctcaaaattc ttaactatca taccataccg
     7501 tgaaaaagat cgaaagaccg aatacacgca gttcgcaatc gttttatctt tttttacgta
     7561 ttttttccag aaaaagcgaa caacaaggta ttttccaaat aatcgtgcag taaaatgatc
     7621 gcacatacct ttcccatctt tttaacagtc tttgcctacc gttcagatta tcattcgatt
     7681 tcacgaaaat ttcttcctat gactggtcct tttgttaaac ccatcttcta ttcgttttat
     7741 ttccagctca ctgcatggct cttaaaatga ctcgaactag tgatttcatt accatcatct
     7801 tttaatggat agatatgctc ctcttcaaag taaaacgatt ttcttttaaa gtaatagaat
     7861 tgacatggta atgagtatgt atactagtgg tttcacaaat ggtcttttat tcaacatcca
     7921 aaaactgaaa tttctttaaa atccattact aacgaagaaa atgttccttg cttgataatt
     7981 agttaggaca tgtcaagtga acaaatgcat ttacttagcc aaaaaatcta aaaacttcat
     8041 aaatagacaa aaagacacga ctctctaaga agtcgtgtct ttattcaaca aattattata
     8101 atttagtcac ttcagcagct tgaggtccgc gtgcaccttc aacaatcgtg aattctactt
     8161 cttgaccttc ttctaggctc ttgaagccgt ctccttgaat tgctgagaaa tgaacaaata
     8221 cgtcatctcc gccgtctact gtaataaacc caaatccttt ttcattgttg aaccatttaa
     8281 ccgttccgtg ttccataaat gtatagcctc caaaaaagtt cttgcaagta accttgcaag
     8341 aacattatac ccaatttaga aaacgctagc aagctttcgc tcttttattt tccaaattat
     8401 ttttaagact gatatgacgg aggaaatggt tattttgctg cttcgaaacg tttgtttact
     8461 tcatcccaat taataacatt ccagaatgct gcaatataat ctggtcggac atttttatat
     8521 ttcaaataat aagcatgttc ccaaacgtcc aaacctagaa ttggtgtctt tccttccatc
     8581 aatggagaat cttgatttgc agtagaggta atagctaatt ttccattttc cattacaagc
     8641 caagcccatc cagaaccaaa tcgtccagcg gctgcttttt tgaattcttc tttaaaagaa
     8701 gaaaaatcac caaaagcttc attaatcgct tcttttattt ctcctgtagg ttcgccacca
     8761 gcatttggtg ccatgatttc ccagaaaaat gaatggttag catgtccgcc accattgtta
     8821 cgtacagctg tcttgatatc tgttggaata gcgtccatat cagataataa ttcttctatt
     8881 gttttttcgc ctaattctgg gtatttctca attgctgcat ttaaattcgt cacataagta
     8941 ttgtgatgct tatcatgatg cagatgcatc gtttcttcgt caatataagg ttccaatgcg
     9001 tcatatgcat aaggtagatc tggtaatgtg taagtcataa aaaaattcct cctcaatgtt
     9061 ttctttataa tgtctacatt acaaagagta acaaagagaa ggaaattctt caattgttat
     9121 gcctagtcgt ctttttcact ttgacctact tcttcttcaa aaacctcagt ttctttaaaa
     9181 ataaacaact tgctgaaaat atagttcgct aggataacaa taatttgagt gatgattttc
     9241 gccacatact cattgctgtt cataacattg atcaacaata ccattgcccc catgtccaat
     9301 ccgtaagaaa ggatacggta gaagaagaac ttaccgcatt caataagtgc atgtgtaaaa
     9361 ttcggtgatt ttgaatggaa tacccaaagt ttgttcgtta caaaagcaaa caatacagaa
     9421 caaaaccacg aaatagaatt ggataaggcc atacttaaat caaaatattg gcgtgcgaga
     9481 gcaaaactaa caaaatttac aatcgtagcc aaaccaccaa aaaagagata gactagtatc
     9541 tcccaatatc cttttgcttc taaatatcct tttaactgtc gatactgctt catgagccaa
     9601 cctaccttat taaaaattct tatttatcat actctatatt tcaataactt tcatctttat
     9661 ttaaaaaaat taattttttt tgatcctttg cctttgtctc tgcttggatc gtcacatgtg
     9721 taataccata ttttaatttt aataaagaaa ttatacgagg atatatcttt tcaatctcgc
     9781 ttagtagctg atcttcaaaa tcaatatgtc cgtcgaaaat aattatcttt tcgctatact
     9841 gccaaatatg gacatgatga acattcttta ccccatcgat tgcttcaatt tcttgttcca
     9901 ttttttcata gtcaatagca ggtgcacttt gtatcaaaat agcggcagct tttttgatca
     9961 ccattatcgc ttctatcaat atatatatcg aaatgatcag tgtaataact ggatcgatcc
    10021 agtagatatc gaaaaaacgg atcaaaattg ctccgataat cacacttacc gaagacagtg
    10081 tatcacttaa caaatgcaaa taactagatt ttatattcaa actttcatct gcttcttgtc
    10141 gtaataataa tactgagaac aaattcgcta gcaatccaat aactgctact gttagcatca
    10201 aatgactatt tatcttttct ggtgaagtga accgcttgaa tgcttcaacg gttaaaaaca
    10261 aggataatat gatcaatatg aatgagttga tgaaagcact caatatttct aaacgctggt
    10321 agccatacgt acgtttttta tttgcttctt ttcgggccat tttttgtgcc ccgtaagaaa
    10381 aaataattgc caatgtatca ctaaggttat ggaaagcatc tgaaacaaga gacaaactcc
    10441 ctgacataat ccctccaata aattctgata aagtgatcac cgcattcaaa accactactc
    10501 caaatagttt ttttgatgat ctctgtggct gtgtaggcac tattccttcc tccattttat
    10561 ttatattgga tagtctttag acaagaataa catttttttg atatttcacc aatcattcag
    10621 atgtttccct aaagcacttg tatttcactt gatacttcgc tagaattgca agtgatgaca
    10681 ctactagatg aggagtactt atgacaacaa aacaattaat tatcagttcc tttatacgat
    10741 tcaaagacct aaaggaggcg agaaatgtcc cattttggaa agccattgtc tatcttttag
    10801 ttttgagtat aattatggct ttgccaatct cttttcaagt ttttcaagtc ttaaataaca
    10861 ttcaattgga tgggcaaaag attgctactc gcattcctga ttttacaatc caaaacgggc
    10921 agcttcaaac cgaagaaaaa gaaggattca tttaccaaac aaactccatt atcttcacat
    10981 ttgatccaga gggaaaacga acagaacagg acatctctac tgatttgatg gggaattttg
    11041 ttagtgtcgg gatgttaaaa aacaaactta tcatagctct tcctaataca ggcacgactt
    11101 ctgctttgtt gaacaataac caattagaac ttccctacac caatgaatct ttaaaaaact
    11161 taacaggtaa gcagctccgt tcttttttaa gtgaagcatc tataccactt tggataaaag
    11221 tcctgacatt tatattttct atttatccta gttttctgaa tctgctgatc actttgctat
    11281 ttaccaacat tgctgctttt ctctatgcac gctttcgact aacaaaagct acttttcttg
    11341 attgtttaaa aacattgatc tattcaattt cattgcctac tataattgca acaatcttga
    11401 tggtctttct tccttctttt gacactagcg ccttcattgc ttttgcagga atctttattt
    11461 ttgctcaagc tgtcaaagga tgggcaaaaa tatcgatcaa ttaagaaata aagacaactc
    11521 gttatttcta cttaaataga cccttttaga ggttgtgaca tttttttgtc acagcccctt
    11581 ttttatttga aaaatatttc tttctcaaaa aacaagcaag gcgcttcaaa gttcttcttg
    11641 tatgcgctac acttagagta aagaaaggcg ggaaattaac tatgtcagaa agaatcattt
    11701 atgcagatcg tccatttcgt gatgaattta tcgaagaaat ccatactata gcgcctggtt
    11761 atatctttaa aacagaatta caaccagagg atctacaaaa tgtagagatc agtttgggct
    11821 ggaactcaga ctaccaagaa aaactattag cctcttctac cttgaaatgg gtccaatcta
    11881 tctcagctgg tgttgatcag ctaccccttg agactttttc aaagaaacat attttacttt
    11941 caaatggtag tggcatccac agtgaatcga ttacggagca tgtcttagga atcatccttg
    12001 ggtattctcg tggtcttttt caagcacaaa aggcacagag tgacatgaaa tggttaggtt
    12061 catcggtcca ttaccaatct atccaaggga aaaaaatact aatcattgga actggccata
    12121 ttggaaaaat gattgcaaaa aaatgcaaag cattagacat gaactgctat ggaatcaata
    12181 catccggtca tccagcagaa ggaatggaaa agactttttc tttaaatcaa ctaaaggaag
    12241 ttcttcctga agtagatttc gtagtcaata tcttacctct aacggaggaa acaactgggc
    12301 tctttgatca gcatatgttt gaacactttg atccaaaatc tgtattcatc aatgttggcc
    12361 gtggtgcttc tgttaagaca caagatctgg tgcaggcttt aaataaccag caactttcct
    12421 ttgcagcatt agacgttttt gaggaagagc ctcttccaca agatcatcct ctttggaaaa
    12481 tggacaatgt actgattact tcccacattg caggtttgac acctgatttt caaaagaaat
    12541 taatggctat ttttttaacc aatctgaaaa gctactttgc cacccatgag cttcaaacca
    12601 atcaagtaaa attgtctgca ggatattaat agctgaaaac aatattttca aaagaaaaaa
    12661 gagaaggtga caaaaattgt gtcaccctct ctttttttat tgttttttaa agtgataggt
    12721 tggctcctcc aggttgggat cataatcaac aagtaaatca tatcctttaa agaaccattc
    12781 atctgcttcc tcgataaaaa aaagtgtccc atccatttcc actttcttta caggatgttc
    12841 tggctgttca actgacattc caatcgaaaa accatcatga acctctgttt tgccgtagac
    12901 ttttccaaaa aaacgtattc cgtaagtttc tggcagatca atttctgagt caaaccattt
    12961 ttgtgctttt ggcgttaccg ttaatttcat cccaacaacc tactttctct tttctttcta
    13021 ttgattcatc ttatcgtcta gaatatccac atcgcctgtc tctccaataa ccagtagatt
    13081 atcattttca cgaacatatt catctgctgc aggagaagcg ataaccgttt gatctactct
    13141 tcgtatggcg actactgtta agtcaaaacg ttgcctgaaa tttaattcag ctaatgtttt
    13201 gttgaaaaat ttaggattgg agactcggat ttctgctaaa gaatatttat ctgacagctc
    13261 taagtagtct agaatattct ttgatactaa gttatgtgca atccgtatcc ccatgtctcg
    13321 ctcagggtgt acgacacgat ctgctccgat cttctctagt acacgggcat ggtactcatt
    13381 ttgggctttt gctaaaatat tcggtacgcc catttccttt accatcagag taactaagat
    13441 gctggcttgg atatcttctc caatagcgac aattacatga tcgaaatttc ttatacctaa
    13501 tgaacgaagt gtcatctcat cctgtgcatt tgccacaaca gcatgggtgg ctatattcat
    13561 atattcattg actcgatctt cattactgtc aatagccagt acttcttgtc cagcttcgat
    13621 cagtgtacgg cagatgcttc cgccaaatcg tccaaggcct acaattgcaa aattttgttt
    13681 caccattttc ctcctttagt atcttctttt ctcagtataa caaagccttt ccaaaaacaa
    13741 aagcaagcat cattattgta aaaaagccaa gaaaaataat agttattttc tcagcttttg
    13801 attatcctct ttatcatcgt ttttatttca ccaaaccatg tttaaacgca tagatagctg
    13861 cttgagttcg gtcttctacc tctaacttag ctaatatatt cgacacatgc gttttgactg
    13921 ttttcagtgt gataaacaat tcatccgcga tttcctgatt acttttccct tcagaaatca
    13981 gcatcaaaat ttcattttct cgattcgtta attcttcatg caatactgga tcatttcgcc
    14041 gactcatttt ttccatcatt tttgttgtca cttcaggttc tagtactcgc tcgccgcgtt
    14101 gcgtagccct tatagcatca gctatttcat gtgctgtaga cgtcttcaac aaatatcctg
    14161 ctgctcctgc ttcaattgca ggataaactt tttcatcatc aataaaactt gtgacgataa
    14221 tgattttggc ttttggccaa tcttttaaga ttgcttttgt tgattcgatg ccatccattt
    14281 cttccatgac tagatccatt aaaatgacat ccggtcgaag tgccatcgcc ttttcatagc
    14341 cttgacgtcc attttcagct tctccaatca cttcaatgtc ttcttggata gataaataag
    14401 aagagacgcc taagcgtacc atttcatggt catctactaa taaaactttt atcatcttta
    14461 tctatgcctc cttcatcaaa gggaccttgg tctccacgct cgtaccttgc cctttaaaac
    14521 taatgatttt tactgttcca ccaataccag ctactcgttc tttgatattg ttcaatccat
    14581 aacttcccgt tttggtttca ttcatattaa atcctgtccc gtcgtcaatg atacgtaata
    14641 aaagattgtt atctatttta tgcaaataga cttctaattc attagcctta gcatgtctta
    14701 atgtgtttga taacaattct tgaacgattc ggaacaagtg atcctcaatg gaactagtta
    14761 attttacatc ttctacctcc cattttagcg aaatctggat tttattctgc aattccttca
    14821 ataactgctc aatgccttgt tttaaacttt tttcttctaa attgactgga cgcagatgaa
    14881 gaagcagcgc acgcatctca gactgggaag cgttgatgat ctctgccacc attttcaact
    14941 gcttacggaa catttctggt gtctcacttt tttctgcttg ctccgtcaat gccgacatca
    15001 tcatcatcgc tgcaaataat tgctgtgaaa ctgaatcatg aagctcacga gctaatcgat
    15061 gcctttctaa ttttaaaatc tcttctttgg tttgtccatc tacatattga ggacggctag
    15121 tcataatctg taattcactg gatacttcga tcattttttc ttttatttta gtaatttcca
    15181 tatcaatatc tttgatgtac agatcatccg acgcgcttgg gatcggctga tgcaaaagtt
    15241 tggattcata attgccagaa gaaagtgcac gaagcttttc ttcgattttc ccgtattttg
    15301 ccttttgaat aagtgataac aatagaaata cgatcaatcc tacacctaat gaaattgcca
    15361 tcaaatggaa gataagcgga acgtataata aacgcgcacg cagcaattct ccccaccaat
    15421 gactttggtt actggcataa aaataagtga aaagtgaaaa tagaataata aggaaagccg
    15481 caatccccga ataaacagct agcattgctt tggatattct tcccatcata ttcgtatcac
    15541 ctcgacatct ccaaccaaag tattggtaat gatcttcaat ctgcgaggat tttcatcata
    15601 atcactgctg cagattttga tctgttcgtt ttttaaggaa aactgctctt cttcaaacaa
    15661 gacatttcct actaatgtcg catgctctaa acgaatagcc actcccaggg gtactaagat
    15721 ccgtgttctg cctatccctt ttcttacaat cacaatatta tcatcttttg gcaaaagtgt
    15781 attgcccaaa tcgatgatag tatcaccaga aatgatggca atgttgatat catcccattc
    15841 atagacgtcg tttccaatac gttggttacc aaataattgc tgtcttttac gctcattatt
    15901 atgtgttttt aattgctctg tttggaccat catgatctgt ttttttcgcc aaaaagcatt
    15961 ttttgtcaaa tctattcctg aaatctctac acctttcagc ccgataaata aaacggccaa
    16021 tacaaccatc atccataaag caggactgtt gactaagcta aaaaaaatga ccaagctccc
    16081 taagatcaat tgaaaatttt gaaatttagt acgaggatat ttgcgcatag ctagatatat
    16141 attgaaaata ccgaatatta ctaaaagcaa aagtccagta ttgtttacga tttgccaaac
    16201 agcaaataac agcagcagtg cttcgactac cacaaaaaaa cgccaagaac tattcatcgc
    16261 tttttctccc tcctgacaca ttctcatttc atcataccta tttttttgac tgttggctat
    16321 aggactttag atgtaagatc gttcagtgaa acctgcaata ctcaaagaga aaagatctag
    16381 agtgcgtcat cagtaagatg aactctctag atctttcagt cagactacgc ttgttctact
    16441 ttgacgattt taactaacat atcgccacct ggtgtagcaa tggttacttc atcattttta
    16501 cgtttgccaa tcaatgcttg cgcgatagga gaatcattgg aaatctttcc agaaaacggg
    16561 tctgcttcag cacttccgac aatcgtgtac tcttcttcat ctccatcagg caactcgatg
    16621 aacgtaactg ttttaccgat cgatacctca tctgcatcta cgccgtcatt atcgatgatt
    16681 tgtgcaaaac ggatcatatt ttcaattgtt gtgattcttc cttcaacaaa tgcttgttca
    16741 tctttagctg attcgtattc ggagttttcg gaaagatctc cgaaaccgcg agcgattttg
    16801 attcgttcaa tgatttctcc gcgtttaact gtttttagtt cttctaattc tttttctaat
    16861 ttttctttac cttctagagt cattggataa actttctcta ccatgtgtaa cgtctccttt
    16921 tttcgataaa ataccgcaaa aggtaatctt ttgaggaata tagtccaaaa aagattacca
    16981 cgcttttctt aaaatgtaaa tgtttttcta aaaatgatca atgactagcc cctgtttttc
    17041 taagaattat ttacatattt ttgcactagt tccatatgtt cttcatacgt tttagcgaaa
    17101 taaacatttc cagtgctcgt atctgctacg aaataataat agtcattttc tgctggttct
    17161 aatactgcct tgattgcctc ttcgcttgga ttgtcaaaag gaccaggccc atagccggta
    17221 ttggtataca aattgtatgg tgaatctaca gctgtatctt catacgtcac caattcttta
    17281 tgttctccta atgcatataa aacagagata tctgactgaa gtggcatatt tgccttgatt
    17341 cggttaaaga agacttgtgc aatattttta cgatcatttt cttttacgcc ttctttttca
    17401 actagagaag ccaaagttaa aacttcttgt acagtcaaat ttttctgttt gatagctgaa
    17461 taatagtttt gcatgaccgt atttgttttg ttcaccatct caatcacaag atccttaagc
    17521 gaagtactcg tgtagtaatc ataagtagct gggaataagt agccttccaa acggtatttt
    17581 gtgttctcag ctttagaggc actttccaac aattctggga atttttgata caactcgtta
    17641 aagaaagtct catcatttac taaatctaaa aattctttct tgtctttacc agttaccttc
    17701 gctacccgct cagcaatctg atcgatgtcg tagccttctg gaatggcaat tttcccatct
    17761 gcaacttttg ttggctcaga agtgccgcct tcttgcagct gtttgccaat ctcgtcaaga
    17821 gtcatattag gcgctaactg ataatagcca gcttgaaagc cagttaaatt tttaaatttt
    17881 gtataataat tgaaaacaat cccgctttta ataatattgt ccttctccaa aatctcacca
    17941 atctgcttgt tagaagagcc actcgggatc tccacttgga ctaattggtc atctgattta
    18001 tctaaaggct tcaacccgct atctacataa cgatacacgg taaatccaag aaatccgcca
    18061 ataattaata atgtaagagc aatcactaaa atgatcttcc tcacaatctt atcttctttt
    18121 ttacgcagat ttctccgctt gtcgattata tctgctgtag ctgccttact ggcttctttt
    18181 ttatctttac ttgggtgttg tttgtttcct gacaaaacaa ctcctcctta ctatcatctt
    18241 cgtaactctg tttattatac atgaaatttc tactgccgtg aattatttta ccaaatatta
    18301 tgtcaaaatt gtaagtggca aatactcttt ttgaggatta caatgaaaaa tagttaaaaa
    18361 tggatagaaa ggaattaaca tgtcaaaagg aaaacatcga aaaaaacaaa aaatttctat
    18421 ttcttttctt gtaattttat tattgtctgt cagcttcttt tcaacaggca tcattatagg
    18481 aggtcaatta acaaaaatac ctcattccca aacttataaa gcggagccaa cacaacttac
    18541 ttccaattta acgcccgatg agcagaggct tgctagcaaa attgatcaat tactacaaaa
    18601 atcagattat atcggttcga tttatgttcg caaaaataat cgcgtactat tagaaaaagg
    18661 atatggatat gcaaatctgc gcgtaaaaga atcgaattca ccttctattt actatcaaat
    18721 cggttcgatt caaaaagcaa tgactgcttt actgatacta aaacaaatcc aagctggtaa
    18781 gttaactttt gacacaaagt tagcagaatt ttatcctcag attcctggaa gtcagcaaat
    18841 cacaatcaaa aatatgcttt atatgcgctc tggtcttcat cgaactgctt ctcccgacaa
    18901 acctatgtcg gatagtgaaa tcattcagtt cgcccttcat catttgaaac ttactgatta
    18961 tgatacgtat cattatgaac cattgaatta tacattgctt acagggatat tgatcaaact
    19021 aacgaatcag ccttatgaaa ctcttctaaa aaaagaaatc attcgtccac tccatctaga
    19081 acatacagat ttttatgaca atgtaaaaaa ttcgcctcaa catgctgtat cttaccagat
    19141 gtctgcaact gacgattatt caaaagcatt gatcgagcct gaaaccgaca tccgaaatga
    19201 attaggtaca ggaaacataa gtatgtctgt ttatgacttg aacaaattct ttaccagtgt
    19261 tctttctggc ggtatcatac caaaagaact acttttctca ttgtggcaaa ataatggtga
    19321 taaacatcct tacagcggcg gtgtttacag tggaaacgat tatatccttg ctcaggggaa
    19381 tatcaatcga ttccatgcag tagtggcgat gaaaaaagac actaaagatg caatcgtcat
    19441 ggaaagtaat atccaatcgg ataagaacat caaattgccg gccactgatt taagaaatca
    19501 gatttatgaa ctgatggaag gtgttcactt atcaaattaa attttgacat aatattagtt
    19561 gtcatctctg tatagtcaat ttagttagta ctctatctga aaagtagaaa taaattaaca
    19621 aatattatct gtccttatta tttgttaatt tatttcttat ttt
//
LOCUS       DAIGOK010000048        19583 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.048, whole genome shotgun sequence.
ACCESSION   DAIGOK010000048 DAIGOK010000000
VERSION     DAIGOK010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19583
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.048"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(183..347)
                     /locus_tag="NOA01_001355"
     CDS             complement(183..347)
                     /locus_tag="NOA01_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632557.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            642..1970
                     /locus_tag="NOA01_001356"
     CDS             642..1970
                     /locus_tag="NOA01_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HCI0632558.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2172..3005
                     /locus_tag="NOA01_001357"
     CDS             2172..3005
                     /locus_tag="NOA01_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCI0632559.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            3021..3758
                     /locus_tag="NOA01_001358"
     CDS             3021..3758
                     /locus_tag="NOA01_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HCI0632560.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3913..4881)
                     /locus_tag="NOA01_001359"
     CDS             complement(3913..4881)
                     /locus_tag="NOA01_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCI0632561.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5113..5946
                     /locus_tag="NOA01_001360"
     CDS             5113..5946
                     /locus_tag="NOA01_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCI0632562.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5980..6819
                     /locus_tag="NOA01_001361"
     CDS             5980..6819
                     /locus_tag="NOA01_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCI0632563.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6845..7405
                     /locus_tag="NOA01_001362"
     CDS             6845..7405
                     /locus_tag="NOA01_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCI0632564.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7604..9325
                     /locus_tag="NOA01_001363"
     CDS             7604..9325
                     /locus_tag="NOA01_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HCI0632565.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9490..10632
                     /locus_tag="NOA01_001364"
     CDS             9490..10632
                     /locus_tag="NOA01_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCI0632566.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10648..11859
                     /gene="thiI"
                     /locus_tag="NOA01_001365"
     CDS             10648..11859
                     /gene="thiI"
                     /locus_tag="NOA01_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCI0632567.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            12432..13079
                     /locus_tag="NOA01_001366"
     CDS             12432..13079
                     /locus_tag="NOA01_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCI0632568.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    13208..13424
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13472..16117
                     /locus_tag="NOA01_001367"
     CDS             13472..16117
                     /locus_tag="NOA01_001367"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCI0632569.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            16427..17740
                     /locus_tag="NOA01_001368"
     CDS             16427..17740
                     /locus_tag="NOA01_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCI0632570.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVITGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            17730..18383
                     /locus_tag="NOA01_001369"
     CDS             17730..18383
                     /locus_tag="NOA01_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCI0632571.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            18438..19130
                     /gene="radC"
                     /locus_tag="NOA01_001370"
     CDS             18438..19130
                     /gene="radC"
                     /locus_tag="NOA01_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCI0632572.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            19280..19480
                     /locus_tag="NOA01_001371"
     CDS             19280..19480
                     /locus_tag="NOA01_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCI0632573.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         6593 a         3304 c         4151 g         5535 t
ORIGIN      
        1 tgactgttaa tcatgatgtc gtaggttcga gtcctactgc cggagtaaat aaagcttatg
       61 caaagaaaaa gaaatccaat cacttgggtt tctttttctt ttattattat gatgaatcaa
      121 gctttctgtt tttgtcacgt atgttgtaat ctttacgaaa gagtagagtt ggctactcat
      181 aatcaaccat acttcccatc atcgctaaat acgaaataaa tcagcattcc tacaccaatc
      241 aaagcacaag ctgcaccaag aaaagggatg acattcccaa agttatctgt attcataagt
      301 atgccaccac ccacaaacaa aaagccgcca gcaatcaggt ttttcatttt attcatctcc
      361 tttcttacgt tatagtattt ccttctttca atgagtattt tgaaagctat cttattgtat
      421 gatgttcttc taggcctctt ctttattata caggccattt tcgttatctc atagaaatat
      481 tcctttttat ctgaaaacta ttgtataccg tatgacattt ttcttcatga agttgtaagt
      541 atattctcat tcaaatgaaa atttcatcat agaagagatt gtatttctaa attcttaaga
      601 gtaaaatacg aagtataact tggatttgga aggatgattg gatgaaagct attacgcctg
      661 atatgacaac aaaatttaac aaacagtttc aagcaaatat caaacagcag gcattgcagc
      721 gggcagttgt gaagaacgga attactgctt ctgctgaaaa tgtttcagca catgtggaga
      781 atacaccggt attttctata gatttggcta cggggaaagt ggctaatcaa atgcaatcgg
      841 ggcgttgctg gatgtttgct gcactgaaca cattccgtca taagatgctg aatcaattcc
      901 atttaaaaga gtttgaatta tcacaaaatt acactttttt ctgggataaa tatgaaaagg
      961 caaattattt ctatgaaaac atcattgcaa caggaaaaga ggcaatcgat agccggaaag
     1021 tagctttctt actcgctact cctcaacaag atggcggcca atgggatatg atcgtttcat
     1081 tattccaaaa atatggcgtt gtgcctaaat cggtgatgcc ggaaagtgcg aatagttcaa
     1141 attctcgaga tttgaataac tacttgaaca aattactgcg taaagatgca gtagtgttac
     1201 ggaaaatggt tgctcaagga gaaacattag acgctattga agaaaagaaa gaagaaatgc
     1261 tagaaagcat ttataacttc ttagctatct cattaggaac acctccaaaa gaattcgatt
     1321 ttgagtaccg cgatgaagaa aagaactatc atcttgaccg aggactgaca ccacaaattt
     1381 tttatgataa atacatcggt gtaaaattag atgattatgt tagcgtgatc aatgcaccaa
     1441 caaaagacaa accattcaat cgttcttata cagtagagat gctaggaaat gtcgttggcg
     1501 ggaaagaagt caaatatctg aatgttgata tggaaacttt caaaaaatta gcgattgctc
     1561 agcttgaaga aggagaatct gtttggtttg gttgtgatgt aggtcaatct tctactagaa
     1621 ctagtggcat catggcctta gatgcttatt caatggatga tctatttgat acggatttta
     1681 caatgactaa agctgaacgt ttagattttg gagaaagtct gatgacccat gcgatggtat
     1741 tgaccggtgt tgacttagta gacgggcaat caacaaaatg gaaagtcgaa aatagctggg
     1801 gcgaaaaagt tggaaagaat ggcttctttg ttatgagtga tgcctggatg gacgaatata
     1861 cgtaccaaat cgttgtaaga aaagatttat tgacgaaaga gcaattaaat gcgttcaatg
     1921 aagaaccaat tgttctatct ccgtgggatc caatgggtgc tttagcatag acgagtattt
     1981 caatgaagag tatgtagtaa agataaaaag agctggctta agccagctct ttttatcttt
     2041 aaagtccatt ccctgttttt tatggtccct gtgctataat tctaaataaa ggataaggat
     2101 tggaaataat ccttttatca ttaaaaatat gatgacgaag tagaataaaa ttgggagtag
     2161 gggggaaagt catggatatc caaactgttt tatctgatct gaaattaaat ggtaaagtga
     2221 ttccagttgt tggtggcgat gttaatcaga cctatcgtat aaaaaccgaa catcgtgctt
     2281 attttttaaa aatccatccg aatgtaaaaa aaggattttt tgaagcagaa gtagacggat
     2341 taaaagaact gtcagcattc gtacgtgtac cggatacgta tatgctaggt gaaacaagtg
     2401 aaggcgctta tttgttaatg gaatggatcg agccgggaaa aggggatcaa cgagatcttg
     2461 ctgcagcttt agctaatctc catcagcaaa cagcgccaca atttggtttt cgtaaagata
     2521 actatttggg tacacttgtt cagaaaaatt cttttgaaga agattggtgg acttttttct
     2581 ttaaagatcg gcttgaatcc cagatttctt tggcagaaga aacgaatcga tggaatgtac
     2641 agcgtcaaga aaagtatctg cgatttaaag aacgtgtatt gaaatcggtt gaaccaaaaa
     2701 agatcactcc acgcctcttg cacggagacc tgtggagtgg taatgtgttc tttgaccagc
     2761 aagggcatcc agtttttgtc gacccagctg tttcttatgg gaatcgggaa caagatattg
     2821 caatgagcca gttattcggt ggtttccgac cggaattttt agatgcttat caaactatat
     2881 ttccattaga aaagggatgg aaagaccgac tgccgattta ccagttatat tatcttttgg
     2941 cacatttgaa tatgtttgga gaaagttacg gttcccaagt agatcaatta ctagaaaatt
     3001 tttaagagga gggcgtgttt ttggcaaaaa aatataccaa aaagcatgaa gtttcatatt
     3061 atgagtgtga catcaaccaa acgatgacat ttccttcgat gttaggagta gttatcaaaa
     3121 cgtctgaaga tcaaagcgac cagttgggaa gaggcagtga attcgtcaat agctttggac
     3181 ttacttgggt catcacgaat tattccatgg agattaatcg tctccctaga gtcggtgaag
     3241 taatctcagt aacgactcaa gccatggagt acaataagtt tttctgttat cggaacttct
     3301 ggatccatga tgaagatggg aaagaattag tcaaaataga atctgttttc gtattgatgg
     3361 attttgtgaa tcgaaaaatg agcagtgtta atgaagagat cattgctcct tttgagagtg
     3421 agaagatcaa gaaaatcaag cgtcaggaaa aaattgaaaa gattgattcg ggcttcatgt
     3481 tgccatatcg tgttcgtttt tatgatatcg atagtaatca gcatgttaat aatgcgatgt
     3541 attttaattg gatcattgac gtattaggat atgacttttt gactacgcat gttccagaaa
     3601 aagtaattat tcgatttgat aaagaagtag agtacgggaa tgagatcgaa agccattttg
     3661 aacaaatccc tatcgaaaat ggtgtcaaaa caaaacatga aatccgaatg caagaacagc
     3721 tgtattgtga agcaaatatt atatggaaaa ataaataaaa aagaggttgt gacagaaggg
     3781 ggcaacttca agaaataaga agaaatttac gaaaattgtt tctcaaattt ttgtaaattt
     3841 tcgacttagt tttcgaagaa gctatttttg tcacagcctc tctttttaag tttagctatt
     3901 agataatgat gtttagtttt ggataaacga aatcaattct tcacccgaca aaccatagtt
     3961 tacagcaatc aatagtttta aacgtgcttt ttgactatta atactgttac agaaaataat
     4021 ccccattttt tctagttctt ttcccccgcc ttcataatca taaacaggct ctgcgattcc
     4081 attgaaacaa cgagaaacaa gtactacagg gattttttgg acaagaagct tttccaaagc
     4141 agctaaggct tgtggaggga gattccccgc acctaaagct tcgattacca aaccgtctac
     4201 tttcgtatgg gcgatggctt ccaataagtc cccttgcatc ccagcatatg ctttaacgat
     4261 cgggatagtc ccatctacag cggaaatgtc cagtcgcttt gtttctaaaa gttcctgcaa
     4321 gaataaaatt cgattcttag ttactaagcc gatgggaccc aatgtaggtg ttcggaaagt
     4381 agcaacattt gtggtatggg tctttgtcac gtagcgtgca gaatggatct catcgttcat
     4441 gaccaccaaa acccccttat ctagcgcttc ttcacaactt gcgacacgaa tcgcactttc
     4501 aaaattataa aggccgtcac ttccaagttc attacttgaa cgcattgccc cagtcaaaac
     4561 aattggtaat ttgttgccaa tcgttgtatc aagaaaaaaa gcagtttctt ctaatgtatc
     4621 tgtaccgtgt gtgatcacca ctccggatat acctgatgca taagcggaca agatacgttc
     4681 ttttaatacc aacatgtgag aaggcatgat gtgtggtgag ggaagattga agatatcttc
     4741 aactaataat tctacatgct tggggattgt gatttctgca tctaataatg ggtttgccac
     4801 atcaggacta acaccgcccg tgagcatgtt ttctttcatg gcgatcgttc cgccggtgtg
     4861 tagtacaagt atttttttca ttttacagac ttccttacga ataattttgc atagccgtta
     4921 aatcttattg tatactatat aaaagagttt tgctatcagt gaagaataac gaatagccta
     4981 acttatcata aaaaagctga ccttattaaa atggaaaaga ctcaaccttg acctcctaat
     5041 acaatggagt atctggttga gtttctttta caagaaagca gccaaggaaa tcaatcacga
     5101 aggtggccgg aaatgatcag aatcggatta acatcgttta aagaacatga caaattaact
     5161 ggtaaaaaga ataacactct ttatgaatat gcagggtttc tacccttagt ggaattagat
     5221 actgcctatt atggaattcc ccgaaagtcc tcagttgaga attggataag tgaagtacct
     5281 gaatcatttc gctttgtagt caaagtctat caaggaatca gcggtcaagg agactggaag
     5341 ccttattatc agaccgaaga agagatgatt gcgcattttt tagatgttat gtcgccaatg
     5401 gtagatgctg gaaagatgtt ttgcttttta gtccaattcc cctcatcatt caaatgtacg
     5461 aaagaaaatg tccagtactt gcgcaaactt agaaagtggt ttgagggtta tcctgtttct
     5521 atagaattgc gggattattc ttggtacagc gatgaatttc ttgaacgtac aagaaacttt
     5581 atgtctagag ccgctttttc tttagccatc atcgatgagc cacagttgct gaatacaacg
     5641 attccttttg atccatttgt caccaatccg aactttgctc tttttcgatt ccatggtagg
     5701 aatgcgcaag gatggcaagc aaatgataaa gagtggcgta aaaaaagaac gttatatcgt
     5761 tattcagaaa aagaactgca agaactagca catgaagtca aagtagttga ttctaagaca
     5821 aaagaggtag gtgtgatttt taataataat tctggtggtg atgcggctga aaatgcatta
     5881 gcactgcaaa aaatattaaa tatcaagcca gatggactca atccaaccca aatggatctg
     5941 ttttaagggc ttattttatt caagaaaggg gcgaagtcta tgatcaaagc tatttttttt
     6001 gatattgacg gaacattggt caccaatcgt tcaaaagcgt tggaatcaac aaaacaagca
     6061 attgaatatg ctcggaaaaa cggcatttta tgcggtgtag caacggggcg cagcccagta
     6121 aagatcaaag aaatcattga tgaattggaa ttagatatgt atgtcgtgta taatggtcaa
     6181 ctggttttta ctgcagatcg cacgattatc gaccaccctt ttgaacaaaa agtattggaa
     6241 catattgttg aatttgcaga tgagaaccat cgacaaatcg tttttggtgc gagaaatcgg
     6301 ttagatggat caacgacgat gctattagga caatcgattt ttatcaagcg actagtcagc
     6361 tttttaccgc gtaaattccc ggtacgtctg atgaaaaaaa tcctccaggt ttttagccct
     6421 caccgacaaa aggatcgtta tgaaacgtta gagatattaa aagaaccaat ttatcaatgt
     6481 atattgctca gtccggagtc agaacagaaa aagttagaac atcgctttcc cgagtgtacg
     6541 tttcagcgtt caaatacgta tactgtagat atcatcccaa aaggtggttc aaaattacta
     6601 ggcatccaag ctttcgcaaa tgctgtcgaa attgaaatgg aagaaattat ggcattcggc
     6661 gatcattaca atgatatcga gatgctgaaa ggtgtaggaa ttggagtggc catggggaat
     6721 gctcagatcg aagtaaaaca tgctgcagac tttgtgactc aatcaaatga agaagatggg
     6781 atatacgaag gactgaaaca cttcggtctg atttattagt caatgaaaga aggaaaaaat
     6841 cagtatggaa gcagagacac cttttcaaaa agtagaaaca ttccacaaaa cttttgatcc
     6901 aagaagacca gagataccca cggcttttac agaaaatgaa gctcatacac gtgctgcgtt
     6961 caaaatagaa gaacttgttg aatttgttgc agctacttca cctaataaag aacaactgca
     7021 tgaagctgtt atgctgctgc acgaagcatt agataaagca gaagaaaaaa tacttggtaa
     7081 aacacagtgg ggaagtgatc cgctggttga acaagtagat gcactgacag atatccttta
     7141 ctttacttat ggttcttttt cattactagg aatagtgcct gatcgaatat tcgaaatcgt
     7201 ccatcaggca aatatgggga aattatttcc agatggaaaa cctcattatg accctgtaac
     7261 aaataaagtg ttgaaacctg aaaactggca gatggaatat gctccagaac ctaaaatcgc
     7321 ggcagaaatc caacgtcaga ttcaagaagc gattgctaag aagaaatccg ctgatatagg
     7381 catgtgcaca aatgaaaaag agtgatcaga gtttcactat ttttgttatc tttatacgat
     7441 ggaaaaacaa aagattttcg tttaagttag tgaatgcatg aaaatagtat gctataatct
     7501 agaatagatg tcttttgcac gagagacgta ttatttggat ttatgccaag cagaacgcca
     7561 tctgttgcat aaaagatgca tactgaaaac ggaggaacca taaatgggaa ataatgttat
     7621 catcggaatc gtcattgcaa tcattatagt cgccattgtc gtctttttgg ttagttactt
     7681 gatgaagaaa aaaaaccaag atcgattgga tgccttagaa aaacgcaagg aagaattatt
     7741 tgacttgcca gtgattgaag aagtcgatga tgttaaaaaa atgcatctgg ttgggcaaag
     7801 ccaaaatacc tttcgtgaat ggagtcaaaa atggaatgac atatctactt cctcgttcgc
     7861 tgaactagaa agtcaaattt tcgaagtgga aaacctcaat gaaagttttc ggttcttaaa
     7921 aggaaaaaaa gcagttgttg aagcagaagc tacaatggac caaatggaga aacaagtgac
     7981 ggaaatccgt gaaggtctaa aggaattgcg ggaaacggaa gaacgcaact cactagctgt
     8041 tcagcaggca cttgacgtct atgaagaact gaaagaaaaa gtcagagaag acgagcatga
     8101 atacggacca gctctttttg aattgcaaaa acagatcaaa gtgatcgaaa aagaatttac
     8161 gcagtttgtg actttaaaca cttctggaga ccctgttgaa gcccgcgagg ttttggataa
     8221 agcagaaaaa catacgtatg aagtggaagc tctgatgaaa aaaattccac cgctgtatga
     8281 ggagctccat accactttcc ctgaacaact tgaagaaatc agtgatactt acgagaaatt
     8341 gatggaagaa caatatgtat tcccagaaga agatcttgct gaagatatcg caaaagtaag
     8401 tcgaagaatt gaaaattcat tagccaatct tgaaaaaaca gaagttgaaa cggtcgaatt
     8461 tgaaaaccgt gaaacagctg atttgattga ttcgttatat gatatcttag aacgggaaat
     8521 ggaagctcag agatatgtaa aaactaacca atcaacgatt gcagagtata tcaaacatac
     8581 aacaaagaat aaccgtcaat tattgattga gctggatcac acatctcaaa gctatacatt
     8641 gaaccacaat gaaattggcc gtgtacgagg tttccaaacc gaagtggaag aaatggaacg
     8701 tcaaaatgaa cagatgattc cacagatccg tcaacacgaa attccttatt cagaagtgcg
     8761 cacgttctat aaaacagtct tcaaagtctt agaagatatc gaaaagcaac aagtagagat
     8821 cgacgactca ttacatgaat tgagaaaagg cgaaaaagaa gcgcaagaaa agatcgatac
     8881 attcgaattc aaattgcgca gtttgaaacg ttttgtagaa aaacaacgtt tgcctggctt
     8941 gccgaatgat tacctagaat tcttttttgt tgctactgac cgtattgaag aactaagtgt
     9001 tgtcttgaat aaaattcgag taaacatgga agaggtcaat cgtttagttg ctctatgtga
     9061 agaagatcta gaattattag ataagaagac acatgattta gtagatgcag cagcattaac
     9121 ggagcaaatg ctccaatatg caaatcgtta tcgtcataca cacgaagaag tgagagaagc
     9181 aattgataaa agctactatt tattcaataa agaatatcac tatcaagaag cattagacga
     9241 aatcggtaca gcattagagc gagtagaacc tggtgctttc aaacgcatcg aagattttta
     9301 ttttaatcat ccagacttag tataaagcaa aaaagttagt aaaccagcaa gagcaagacg
     9361 gattattgtc ttgttcttgt ctttttttgt atatttgaaa attgaaacaa gtgaaaattt
     9421 cacctgctct tgaaagcacc ttggacaacc gttataatag aaaagaattt caaaaaagag
     9481 gggtttctta tgatatattt tgataacagt gcaacaaccc caatctttcc gcaaagctta
     9541 gatgcatatg taaaaaccag tcagcggatt attgggaatc catcaagctt gcatgattta
     9601 ggcaatcaag ctaaccggtt gttgcagcaa gcaagaaagc agatcgctga tttgatccat
     9661 gtagaaccag aagaaatcta ttttacttca ggcggaacag aaggagataa ctgggtatta
     9721 aaaggaacga tgatcgaaaa aagagaatat gggaatcata tgatcatttc aggagtcgaa
     9781 catccggccg tcagcgagac agcagaacaa ttaaaagaat taggaatcga agtaagtatc
     9841 gccccagtgg ataaacgcgg atttgtacgt gtcgacgaat tgaaagaatt aataagaaaa
     9901 gagacagtat tagtctctgt tatggctgtg aataatgaag taggatcgat tcagcccatc
     9961 caggaaatca gtgatctttt ggatgatttt cctaaaattc atttccatgt agatgctgtt
    10021 caggcgattg gaaaagtacc attctcgcaa tggttgacag aaagagttga ttttgcaaca
    10081 ttttcagctc ataaattcca tggcccacgg ggaactggtt tcatttattg gaaaaagggt
    10141 cgtcgtttag caccattact gacaggtgga gggcaagaaa gaaatcagcg aagtgggaca
    10201 gaaaatgttc caggtattgt ggctatggtt aaagctttac gtttatcatt agagataaag
    10261 gaacaaaaac ctgagcagac acaacgctta aaagtagctc taatcaatgc gttgaagacg
    10321 tatgagaaag tgacgatttt ttcagaagga actgactatg caccgcatat tttatgtttt
    10381 gctctaaaag gaattcgcgg cgaagttctc gtccatgcct tagaagaaaa acaaatattt
    10441 gtttctacta ctagtgcctg ctcctcacgt aaacatatgg caagcagtac attgcacgca
    10501 atgcatgtac caaatgatct ggctacatct gctgttcgaa tcagcttaga tgaaaacaat
    10561 tcgcttgcag aagtcgagca atttatgatt attttcaagc agctttataa gaaattttct
    10621 gcaatcaatt aaaagaggag tgttcttgtg aaatacagtg aaattatggt ccgttatggg
    10681 gaactttcga caaaaggaaa gaatcgaaaa agctttatca tgcaactagc acaaaatgtc
    10741 agacaagctt tgaaagactt tccagaaatc aaaatccatg ctgatcggga ccggatgcat
    10801 cttttattga atggcgcaga cgggcagcta gttattccaa aactagcaaa aatatttggg
    10861 atccaaaatt tctctccaag tattcgtgta gaaaaagatg tcgaagtttt gaaacaagct
    10921 gttcaagaca tcatgaaaga aatttatact gggaaagaaa catttaaaat tatcgctaaa
    10981 agaagcgatc accaatttga attgacaagt aatgaattga atcaaacatt aggtaatgct
    11041 gtttttgata ttttcccgca tatccaagtt caaatgcgtc aaccagatat tccattacgt
    11101 gtagagattc ggcgtgatgg cgcttatcta tcgtatgaaa cgatcaaggg agctggagga
    11161 ttgcccgtag gaacaagcgg aaaaggaatg cttatgcttt ctggggggat tgattctcct
    11221 gtagcagggt actttgccat gaagcgggga gtggaaattg aagctgttca ttttgctagt
    11281 ccgccgtata caagtgaaca agcactgcaa aaagcaaaag atctaacagc caaactagca
    11341 ccttatgtgg gaacgatcca ttttatcgaa gtgccattta ccgagattca agaagaaatc
    11401 aaaaagtcag tgccacaagg atattggatg acaattactc gtcgaatgat gttgcgtttg
    11461 accgatgaaa ttcgccgtat tcgtcatggt ttagtcattt tgaatggtga atcattaggt
    11521 caagtggctt cacaaacttt gcaaagtatg gtggctatca atgaagtaac gaatacgcca
    11581 atcattcggc ctgtagcgac aatggataaa cttgaaatca ttgaagtagc agaacagatc
    11641 gatacattta atctttctat ccagcctttt gaagattgct gtacgatttt tgctcctcca
    11701 cagccaaaaa cacgccctca actggaaaag gtccatcaat atgaagagcg tttagcaata
    11761 gatgaaatga tccaacgagc actagctggc ttgaagatgg aagagataca agcagaatcg
    11821 tctaatgaaa aaactgaaga attttcagaa ttgctataag aggttatgaa aatagtgttt
    11881 tgaccagagt attagagaaa caaaacgaaa aaatagctcc atgtatttta ccgtttttga
    11941 actaatgtcg aagattagct gattgaatac catttatcaa gaagttatgg aaaagccggt
    12001 tcttttttac taactgaaaa ataaaatttt atttagagat caagctacag ttgataaatg
    12061 atataaaaaa gagaatttcc catgaaatag cgaggttgtg acagaagtgg gcagcttcaa
    12121 gaaataagaa ggaatttacg aaaattgctt ttcaaatttt tgtgaaattt cggcttattt
    12181 ttcgaagaag ttacttctgg aacaccgtgt atcagaggtt gcgaaaaagc tgccctgcat
    12241 caagtaataa gaacagccaa acaaaaatag ttcctcatat tttacgtttg gttgagctta
    12301 ttaccgcaga tgcaagcttt tgaacaccgt tttatttgtc tcacaccctt ttccttccta
    12361 cttgtaacct tgctcccgtg ttagaaacat gttacactaa atatgtgaaa ttattaacaa
    12421 ggagcgtcgt tatgcgagaa aaaacagata cactgccaaa agcaactgcg aaaagattac
    12481 ctttatattt acgctacttg aagatgctgg atgactcagg tataagtaga atcaaatcaa
    12541 atgagttcag cgaaatcacc caaattcctt ctgcaacgat ccgcagagac ttctcgcaat
    12601 taggcgagct aggcagaagc ggatatggct atgatgtccc ttttcttatc gatgtattca
    12661 ataatatcct taatactaaa gaagaaaaac gaatcgcatt agtaggatat ggaaatcttg
    12721 gcaaagcatt gaaacacaac aattttagaa gaaatgaaaa tctaaatatc gtgtgcgtct
    12781 ttgataatga tccagcactg atcaatcgag taattgatgg agaaatgatc tatccgattg
    12841 atcgtttcgc ggagatagcc aaagcaaaaa atgtgacagt agcaatctct acggtaccaa
    12901 gtaaatattc acaatctgct attgatgaaa ttgtcaaagg aaatgtaacc gctatactta
    12961 attttgcgcc tgacagagta actgtcccag cttatgtcaa tgttcaatac attgatttga
    13021 caaccgagtt gcagacgctt atttactttg atgagaatta ctcagaagtt ttttcttagg
    13081 aaaatttgag taggcttatt gatcttgcga gaactcgtcg taattctttc aaaaattttt
    13141 tagtactaaa tgaagtggtt ttttcacgac cacttgttga ctaaaaaaaa aaaccattgt
    13201 agaatgaagc ataacgctga acaagaggag taactggcac attcattctt agagagagaa
    13261 ctatctgctg aaaggttctc aatgacaacc agtgaaggta gcttgggagt ttctgtcttg
    13321 aatggagtag agatagacga gtcgtgatcg ttaactcact tgagaggcaa taatgattga
    13381 ttattgcaat ttaggtggta ccgcgtaaat catacgtcct agaatacgaa agtattttag
    13441 gacgtttttt agtttaaagg aggaaaacag catgacggaa gaaaataacc tatcaacaaa
    13501 atataatccg caagaagttg aagctggacg ttatcagcat tggttagaac aagatttgtt
    13561 taaaccgagc ggagataaaa aagcaaaacc ttattcaatc gtaatcccgc ccccaaatgt
    13621 tactggaaaa cttcatttag ggcatgcttg ggatacgacc ttgcaagata tgctgattcg
    13681 tcaaaagaga atgcaaggat atgatacttt atggctgcct ggtatggacc atgcaggaat
    13741 cgcgacacaa gcaaaagtag aagcgaagct tagagagcaa gggatttctc gttatgatct
    13801 aggtcgtgaa aagttcatcg atcaagtgtg ggaatggaaa gaagagtatg cgagccacat
    13861 ccgcgaacag tgggctaaaa tgggcttatc tttagattac agtagggaac gttttacatt
    13921 agatgatgga ttatctgaag cagtacgtaa agtttttgtt accttatacg aaaaaggttt
    13981 gatttatcgt ggtgaatata tcatcaactg ggatcctcaa gcacgaacag ctctatctga
    14041 tatcgaagtt attcataaag atatcgaagg cgccttttat catatgagct atgaactagc
    14101 agatggatca ggcgtagtag aaattgcgac aacccgtcca gaaacgatgt tgggagatac
    14161 ggcgattgct gttcatccag aagacgagcg ttatcaagcg ctcatcggca aaaaagtgat
    14221 tctaccttta gtcaataaac aaatcccaat cattgcagat gaatatgtag atatggaatt
    14281 cgggacaggt gtagtaaaaa tcactccagc acatgatcca aacgactttg aggtaggtaa
    14341 tcgtcatgat cttccacgcg taaatgttat ggatgatgat ggtacgatga atgaactagc
    14401 aggaaaatat gctggaatgg aacgttttgc tgctcgtaaa gcaatcgtgc aagatctgaa
    14461 agaaatcggt cgattgatta aaatcgatac gatgatccac agtgtgggtc attccgagcg
    14521 gacaggtgtc gttgttgaac ctcggctgtc tactcaatgg tttgtaaaaa tggcgccgtt
    14581 agcgaaaaaa gccattgata accaagaaac agatcaagca gtggaattct tccctccaag
    14641 attcaatcaa acgtttttac gttggatgga aaatgtccat gattgggtaa tctcacgaca
    14701 attatggtgg ggacatcaaa ttcctgcatg gtatcacaaa gaaacaggtg aaatgtatgt
    14761 agggatggaa gcaccagcgg atagtgaaaa ctgggtacaa gatcctgatg ttttagacac
    14821 ttggtttagt tcagctttgt ggcctttctc aacaatgggc tggccagata cggaaagcga
    14881 agattataag cgatacttcc ctactagcac tttagtaacc ggttatgaca tcattttctt
    14941 ctgggtaagc agaatgattt tccaaagctt ggaatttact ggcgaacgtc catttgagaa
    15001 tgtcttgatt catggattga ttcgtgacga acaagggcgg aagatgagta aatcattagg
    15061 taatggaatc gatccgatgg aagtaatcga aaagtatgga gcagatgctc tccgctggtt
    15121 cttatcgaat ggttctgcac caggacaaga tgtccgcttc agctatgaaa aaatggatgc
    15181 ttcttggaac tttatcaaca aaatctggaa tgcttcacgt ttcgttatca tgaatatcga
    15241 aggaatgacc gtagaagaga tcgatttatc tggagaaaaa tcagtggctg accggtggat
    15301 ccttactcgt ttgaatgaaa cgatcgaacg agtaactgag ctatttgatc gttttgaatt
    15361 tggtgaagca ggtcgacaac tgtataactt tatctgggat gatttttgtg actggtacat
    15421 cgaaatgagt aaagaagtac tttatggaga tgatgctgtt tcaaaacaaa ccacccgtag
    15481 catcttagtc catacacttg atcagatcct acgtttattg catccaatca tgccattcgt
    15541 tacagaagaa atatgggaac atattcctca tcagggaaat tctctagtag tggcagaata
    15601 tcctgttgta cgtccagagt ttgatgatga aacagcttct aaagggatgg aagtattgaa
    15661 agaattgatt cgagccgttc gaaatattcg ttcagaagtg aatacaccat tgtctaaacc
    15721 aattacgttg ctaattaaaa caaacgatgc taaaatcgat caatttttag ttgaaaatac
    15781 aagctatatc gaacgcttct gtaatcccga agagttgacg atatcaagtg agatcactgc
    15841 gccagatcta gcgatgtcag ctgttctgac aggtgcagaa atcttcttgc cgttagcagg
    15901 attgatcaat attgaagaag aaatcaaacg attagaaaaa gaactggaaa agtggacaag
    15961 cgaagtaaaa cgtgtccaag gaaaactttc taatgaacgt tttgtttcta acgcgccgga
    16021 agaagtagta gaggcagagc gtgcaaaaga aaaagattat ttagagaagc aaacggctgt
    16081 caaagaacga atcgaaagtt tgcgtactat tcaataagaa tagaaaaaga gagtgtgaaa
    16141 tcagctagct attactaaaa gctagctgat tgaacatcgt tacaggaaag agtttgagcc
    16201 tcactcttag cttcgattca taaggagatg tgacataaat tttgtcgcat ctctttttta
    16261 gtctactctt attatgtagg agaaggaggg aggtggatca tgtatcaaga gtcaataaag
    16321 tagttgataa aatcgttcct ctctctgttt cttttctctt tgtcatactt ttatttttct
    16381 tatgtgttct atttgttata ataaaagaga aatgggggaa ttgttgatga cgattgaaga
    16441 agcaatagaa tggatccata gtcgattacc atttggttca cgtcctggcc ttgaccgagt
    16501 cgaagcttta ttagaagtgg tagggcatcc agagagaaaa gtaaaaacga tccatatcgc
    16561 tggaacaaac ggaaaaggtt ctactgtagc ttatttgaga agcctattag aagaggctgg
    16621 gctgacagta ggtacattta cttcacctta tatcgaaacg ttcaatgaac ggatcgcaat
    16681 caatggcaaa agcattccag acaatgattt gatccagctt gtaaaaaaat atcagccact
    16741 gatcaaacaa cttgatcaat ttgaagcagt agctggtatt actgaattcg agacattaac
    16801 agcaatggca tttgattatt ttttgatgaa gaaagtggac gtagcgatta tcgaagttgg
    16861 cttaggcggc ttattggatt ctaccaatgt agctgtacca atgctgacgg gaataacaac
    16921 gattggactt gatcataccg aaatacttgg agatacaatt gaagaaatcg ctgcacaaaa
    16981 agctggaatc atcaaacaag gcgttccagt aataacagga aatattgccc cagaagcttt
    17041 tactgtcatc caacaaacag ctaaagcaaa aaatgcaccg ctttatgtat acggggaaaa
    17101 ttataaaata aactatctgc atcctgatga gatgtgggga gaagtatttg aattttttaa
    17161 tgaaatagga aagatccctc aattaaccac tccgctttta gggcaacatc aagctgaaaa
    17221 tgcaggagta gccatcgaat tgtattttgt ttactgtcaa ttggaaaagc tgcctttccg
    17281 agaaaaagat gttttgaagg gattgaagaa atctcgatgg ccaggaagaa tggagcgttt
    17341 gagtaacgag ccattagttg ttttagatgg tgcccataat acgcatgcaa tgaaccggct
    17401 tgttgagaat ttgaaaaaag aattcaaagg atatcaaatc aacattttgt tttccgcctt
    17461 acgaactaaa aatgttagag aaatgctaga acgattgcag caagtaccaa atgtccatat
    17521 ttatttgacg acctttgaac atccaaaagc catcgaatta agtgaagtat cagatttagc
    17581 aagtgagaga gtatcaatcg tttcattgtg gcaattcgga ttagcagata tattggaaaa
    17641 gatgacaaat gaagacgtat tagttgtgac cggttcactt tatttcattt cagaagttcg
    17701 ccatttatta atacaattag gaggagatca tgaagtataa tgcagtgatt tttgacatgg
    17761 atggtttgct gttcgacaca gagttagtgt attacgaagc cacacaagta gtagcagaca
    17821 agatgggttt tccttatgat aaagacctgt atttaaaatt cttaggtgtt tcagatgaag
    17881 aagtatgggc gaattatcat atgattttct cggaatttgg caaaacaagt gtacaaagat
    17941 tcatagatga ttcataccag gaaacgcttg aacgtttttc gcaaggagcg gtacaaatga
    18001 agcccggtgt gttggaactc ctctcttttt tagaagaaca tcagattccc aaagttgtcg
    18061 cttccagtaa ccaacgagca gtcattgaac ttttattgaa gaagaacaat ctgtttgaac
    18121 gatttgagac gattgtttct gcagaaaatg ttcaacgagc aaaaccagac ccagaaattt
    18181 tcttgttagc taatcagaag ctaggtacag cgaagaaaga aacattgatt ttagaagatt
    18241 cacaaaatgg tattttagca gcttctgcgg ctgagatccc ggtagtcatg gtaccagatc
    18301 tgcttccacc atcccaagaa ttgatcaaaa aaacagctgg ggttgtttct tctttacatg
    18361 aaatccctgc attgttaaaa taaaaatcca tctcttttcg tatcttattt tagataagat
    18421 agaaaggaga tgtttttttg ggaaaacgat tgatcaaaga agtacctact agctctttac
    18481 caagggaacg tatggaaatt tatggcgcgg aagctttatc ggatcaggag cttttggcaa
    18541 tcttactgcg taccggtcaa cacccttata gtgtgatgtc gattgctgga aatctgttaa
    18601 aaacgtttgg aggactggcc tctttacgac aggctacttt gcatgagtta gaagagattc
    18661 gagggatagg acgcgtgaaa gcaatcgaaa tcaaagcctt gatcgaatta ggtcgacgaa
    18721 ttcagacgga tcatgaaaga gcggctccac cggttcgtac cagctacgaa ttggcccagc
    18781 aattgattct ggaaatgaaa gatcacaaac aagaacattt ggtttgtatt tatttggaca
    18841 cgaagaatca agtgatctta aaaaaaacag tgttcatcgg ttcgctgaat cagtcagtcg
    18901 ctcatcctag agaaattttc cattatgctg tacgttattg tgcagcaagg atcgttttag
    18961 cgcataatca tccttctggt aatgttattc catcgcagca ggatatgaat tttaccaaaa
    19021 ggatacaaaa gtgtggagaa atgatgggga tcactgtact ggaccacttg atcatcggac
    19081 gcaaacgata ttttagtttg agagaagaag gaatgatgga agagaagtaa gttgctttct
    19141 tgcaaaaaaa atgaagcaag tgtacaatta tattgtaatt ttgtttggga atagaagttg
    19201 aaacatctgt tgcacctcta tcgtacacga gcaatgttac aatttaaatt agtgcttttg
    19261 gcacgaggag gatattatta tggaaaacgg aacagtaaaa tggttcaacg ctgaaaaagg
    19321 atttggattt atctcacgtg aagatggaag cgacgtattc gttcacttct cagctatcca
    19381 aggtgacgga ttcaaaacat tagaagaagg tcaagcagtg actttcgacg ttgaagaatc
    19441 agaccgcggc ccacaagctg caaacgttgt aaaagcttaa ttttatctgt aaatcaaatt
    19501 gatggtagac atcaattttt acgagagaac aaagcgagta acaaaaatac ctgccacaag
    19561 agacaatttc tcttgtggca ggt
//
LOCUS       DAIGOK010000049        19316 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.049, whole genome shotgun sequence.
ACCESSION   DAIGOK010000049 DAIGOK010000000
VERSION     DAIGOK010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.049"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(27..1523)
                     /gene="lysS"
                     /locus_tag="NOA01_001372"
     CDS             complement(27..1523)
                     /gene="lysS"
                     /locus_tag="NOA01_001372"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCI0632574.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="NOA01_001373"
     CDS             complement(1634..2638)
                     /gene="dusB"
                     /locus_tag="NOA01_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCI0632575.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="NOA01_001374"
     CDS             complement(2639..3526)
                     /gene="hslO"
                     /locus_tag="NOA01_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCI0632576.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="NOA01_001375"
     CDS             complement(3743..5854)
                     /gene="ftsH"
                     /locus_tag="NOA01_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCI0632577.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="NOA01_001376"
     CDS             complement(5944..6489)
                     /gene="hpt"
                     /locus_tag="NOA01_001376"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCI0632578.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="NOA01_001377"
     CDS             complement(6489..7934)
                     /gene="tilS"
                     /locus_tag="NOA01_001377"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCI0632579.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8054..8524)
                     /locus_tag="NOA01_001378"
     CDS             complement(8054..8524)
                     /locus_tag="NOA01_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HCI0632580.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8570..8995)
                     /locus_tag="NOA01_001379"
     CDS             complement(8570..8995)
                     /locus_tag="NOA01_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HCI0632581.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9062..9331)
                     /locus_tag="NOA01_001380"
     CDS             complement(9062..9331)
                     /locus_tag="NOA01_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HCI0632582.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9342..10937)
                     /locus_tag="NOA01_001381"
     CDS             complement(9342..10937)
                     /locus_tag="NOA01_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCI0632583.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="NOA01_001382"
     CDS             complement(10952..14473)
                     /gene="mfd"
                     /locus_tag="NOA01_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCI0632584.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14490..15050)
                     /locus_tag="NOA01_001383"
     CDS             complement(14490..15050)
                     /locus_tag="NOA01_001383"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCI0632585.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15403..16377
                     /locus_tag="NOA01_001384"
     CDS             15403..16377
                     /locus_tag="NOA01_001384"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCI0632586.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16547..17947
                     /locus_tag="NOA01_001385"
     CDS             16547..17947
                     /locus_tag="NOA01_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HCI0632587.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18025..18747)
                     /locus_tag="NOA01_001386"
     CDS             complement(18025..18747)
                     /locus_tag="NOA01_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCI0632588.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18826..19311)
                     /locus_tag="NOA01_001387"
     CDS             complement(18826..19311)
                     /locus_tag="NOA01_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HCI0632589.1"
                     /translation="MKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLESWLPNQPF
                     KDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGVFPKLKIW
                     WLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVLFAAFKKR
                     L"
BASE COUNT         5260 a         4305 c         3464 g         6287 t
ORIGIN      
        1 gaattcctta gacagctttt attcgattat ctcattgttg ggaatagcaa aacatcccga
       61 attgattgtg catcagttag caacattact aatctgtcga tcccgatgcc tagaccacca
      121 gttggcggca taccgtattc taaggcttcc aagaagtctt cgtctacacc gtgagcttcg
      181 tcatttccta gttcacgttc tttttcttgc gcttcgaatc tttctctttg atcaattgga
      241 tcgttcagtt ctgtaaaggc attagcaaat tcgcgcccca ctatgaatag ttcgaatcga
      301 tctgtgaatc gaggatcttc tggatttttc ttcgctaaag gcgaaacttc gactggatgt
      361 ccgtatacaa acgtcggttg tgtcaatgtt tcttcaacaa atgtttcaaa gaattcattg
      421 ataacatgtc cgacacccat agcttcggtt acttctacat tgtgtttttc ggctaaagct
      481 aatgcttctt ccaatgtcat ttcttgccag aagtctacac cagtttgttc tttgattgca
      541 tctaccatat ggatgcgttt aaagtcactt tctaagtcga cagcttgtcc atcatatgtg
      601 atttttgctg ttcctagtac tttttcagct gcgttacgga taataccttc tgtcagattc
      661 attacatcat tgaaatcagt atatgctgtg tatgcttcta acatcgtaaa ttctggatta
      721 tgtgtagtat caattccttc attacggaaa acgcggccga tttcataaac tttttccatt
      781 ccgccaacaa ttagtcgttt taaatgaagt tctagcgcaa tacgcaaata taaatcaatg
      841 tccagtgcat tatgatgcgt gataaatggg cgtgcggcag ctccacctgc ctcattgtgc
      901 aatactggtg tttctacttc aatatagcca ttgccatcta aatagcgacg gatttcgctg
      961 ataatttggc ttcgtttcat aaatcggtca aagctttcgc gattactgat taaatccaag
     1021 tagcgttgtc tgtagcgttg ttcaatattc gttaagccat ggtatttatc tggcagtgga
     1081 cgaagtgctt ttgacaaaat cgtgatttct gaagctttga tagaaacttc gcctgtgtct
     1141 gttttcatga tttgtccagt tacaccaaag aaatctccaa ggtctgcatg tttgaatact
     1201 tcataggcct catcacctac ttggtctttt cttacataaa tttgaatttg gccttcacga
     1261 tcttgtagat gcgcaaatcc agttttccct tttcctcggc gagtcatcat acgaccagct
     1321 acactagcgg tcaatcccat ttctgctagt tcttcttttg ttcttggatc aaatagttca
     1381 tgcaattctt ctgagttgtg cgtgcgttca aatcgttttc caaatggatc gattccatct
     1441 tcacgcaatt gttccatttt ttgacgacgt acaagcattt gatcatttaa atcttctact
     1501 tgatgttgtt gttcctgtgt cacaaaagtt cctccattct catgttaatc tctttcctat
     1561 aatgccaatg aaacacaaaa aaagcaagcc gaacttggaa tttcggcttg aaaattcacg
     1621 aaaaacttag aaatcacaac gtttgtttca atagtacttt ttccgctttt tctacaaatt
     1681 gatccagcag gtcattgatt tcttgctgtt tttctgtctg attgattgct actttcactt
     1741 tcgctgctcg aggaatccct tttaaataat aagccgcatg tgttctgaat tctctacaag
     1801 ctactttttc tcctttgaga tctgctaagc gttgtaaatg aagtttagct gtagcaattt
     1861 tttcggctgg tgttggttca ggaatcagtt ctcctgtttc gagataatgc tgtgtgcggt
     1921 gaatcatcca tggattgccc agcgctgcac gaccaatcat cacaccgtct gcaccgacat
     1981 agtcaagcat ccattttgca tcttctggcg ttttgacatc cccgttcccc ataaatggga
     2041 tcgtcaaatg ttttttcact tctttcaata cttcccagtc cgcttttcct tcatacatct
     2101 gaacccgtgt acgtccatgc atagcgatgg cacttgctcc agctgattgt gctgctaatg
     2161 cattttctac agcaaagacg tgctcttcat cccaaccaat acgcatcttg attgttactg
     2221 ggatatttac tgctgaagaa acagcttgga ccatttcgta tactttctca ggatctaaaa
     2281 gccattttgc tcctgcttct gctttgatta ctttatttac tggacagccc atattgatat
     2341 ctattatatc ggcagctgtg ttttcttcga caaattgagc agcttcaact agactatctt
     2401 tatttccgcc aaatatctgt aaacttaatg gatgttctgt ttcttcaatg tggagcatct
     2461 ctaaagtttt tttgtttctg aaatgaatac cttggtcact gatcatttca catacgacta
     2521 agcccgcacc aaattctttg acagtgactc gaaatgctga atttgttatt ccagccatcg
     2581 gtgcaacaac gacacgatta gggatttcca cattgccaat tttccatgtg tgttccactt
     2641 aaatccctcc taaggcttct ttcttcaatc catctaattc ttcttcgcta tacttgtatt
     2701 tgttattaca gaaagaacaa accgcttctg caccgtgatc ttcgtctatc attgcttgga
     2761 tttcttctgc acctaacgcg ataattgctt ctgcaaattt ttcttttgaa cagtcacatt
     2821 tgaattgaac aggcattttt tccaaaatgt tcaattcttc tgtaccaagc aatcctttca
     2881 atatttcttc tggtgtctgt ccttcatcta agagggtaga gattcttggt gtcgttttta
     2941 attgttcttc gatcttgctg attgtttctt catcggcacc tggcatgatt tggatcatga
     3001 atcctcccgc cgtcttgatg ctgtcgtcag tattgaccaa tacactcagt ccaacagctg
     3061 aagggatttg ttctgagata gcaagatagt aagtaaaatc ttcagcgatt tctccagaaa
     3121 caatcggcgt ttgcccagaa aaaggttctt ttaatcctaa gtcttttgtg acagtaaaca
     3181 ttccttgtgt tccaactgct ccccgcacat cgattttacc ttcactattt agaggaagac
     3241 tgagatgcgg atttttgatg taacctttca catctccctt tccatttgca tctactacaa
     3301 ttcctgttgc tggaccatcg ccttggattc gaacagttag tttgtcttct cctttgagcg
     3361 tcgcaccaag tagcagacta ccgaccaatg tccgtcccaa agcagctgag gaggtgtgcc
     3421 aagtatcatg acgtctttgt gcctccgcta cggtatttgt tgcttgaacg gcgtatgctc
     3481 ggacgaatcc gtcgtgagca agtgccttaa ccaaataatc tttcatttgt tatgtactcc
     3541 tttttccgtt tttaaactca tagtgaatca tactagcaac tactgctttt ttcaactttg
     3601 gaatgcttct tttaaaaata attcagaatc atctagaaaa caggacatca ccctatttac
     3661 aacttaaata aagaaatctc ccgaaaaccg gtttttgttc cggttttcgg gagtcatatc
     3721 agatcaattg acctatattt ttttatttgt attgatcgtc tgaatgatta tcttctgatt
     3781 gttgagaatc tgaatcatgt tctgctttat tttcctcatt cagttcatgt tttgcttctt
     3841 caaaatcacg tttttcttcc gcttgttttt cagcgtcttt ttcttccaaa gcacgttttg
     3901 cttcttcaaa agttttcgct tctttttcgc ttggataatc atttgactca gtaccttcag
     3961 gcatcacacc gtgttcaaac aatgacttga ttgctttggc atccaatgtt tcgaattcca
     4021 aaagtttttc agcaatcagt ttatgctgtg cacgatgagc ttcgatgatt tctcgagctt
     4081 tatcatgagc ttccatcaag atttttcgta cttcttgatc aatctcgaaa gcgacttgtt
     4141 ccgagtaagc ttttgtttga ccatagtcgc ggccaacaaa cacttgatgg tttccttcat
     4201 attgaacagg acctaaacga tcactcattc catattctgt taccatgctt cgtgctagag
     4261 ctgtcgcttg ctcaaagtca ttggaagcac cagttgattg gacattgaag atgatttctt
     4321 cagctgtacg tccaccaagt aagcctacga tttgttcaaa catatcttct ttcgtcatca
     4381 agaactgatc ttcttttggt aaagcgatca tatatccgcc ggcgcgtccg cgagggatga
     4441 tcgttacttt gtggacgacc cgagcgcgac tcaatactaa tccgacaatc gtatgaccag
     4501 cttcgtgata agcaaccatt tcgcgttctt ttttgctgat tacgcgatct tttttagctg
     4561 gacctgcaat cacgcgatct tccgcttcat cgatatcaga agcatcgatt ttctttttat
     4621 ttcttctagc agctactaac gctgcttcat tcaagacatt ttctaaatct gctccggcaa
     4681 aacctggtgt ctgttgagcc acaactttca agtcaacgtc atctgccatc ggtttattcc
     4741 gtgcatggac tcgcaagatt gcttcacgac cttttacatc tggacgtcca actaaaatct
     4801 ggcggtcaaa acggcctgga cgtagtaacg ctgggtctag tacatcagaa cggttggttg
     4861 cagcaatgac gattactcct tcatttccgt cgaagccatc catttctaca agcaattggt
     4921 tcaatgtttg ttcgcgttca tcatgtccgc cgcccatacc ggcaccacgt tgacgaccta
     4981 ccgcatcaat ttcatcaatg aaaatgatag ctggtgcgtt tttcttcgct gtttcaaaca
     5041 aatcacgaac acgacttgct ccaacaccga caaacatttc tacgaaatca gaacccgaga
     5101 tcgagtaaaa cggtactcca gcttctccgg caacagcttt tgctaataag gttttacctg
     5161 ttcctggagg tccttctaaa agtacacctg ccgggatacg ggcacctaat tcaacaaatc
     5221 ggcgaggatc tttcagaaat tcaacaactt caactagttc ttgtttttct tcttccgcac
     5281 ccgctacatc agaaaaacgt acgcgattag cttttttgtc tgcttctttg gcttttgatt
     5341 taccaaagtt catgacacgg ccgccaccgc caccgccgcc gccttgttgt cccatcatca
     5401 tgtaaaagaa aaagatgatg attacgattg gcaagaagct aagcaatagg gaaacccata
     5461 taccgctgct tgattcttct tttactgttg tagcaacttt gttttctttt gccagttctg
     5521 ttacttggct caatgttgta tcatttggca aaatgattgt tgtgaaatgg gtagtagtcg
     5581 tatctgttga acctaagata gctaacccac cgctgttagc gacggtctgc tgttctttgt
     5641 attcaccagt gatcttatat acaccatttg caggctgcac ttcaaattct ttgatcttgc
     5701 ctgcttgtag ttgttctgtg aattttgagt actcgatatc tgacgatcgc tggccgttat
     5761 taccaaaaat gaaataaacg accattacca tcgctaagat tacgaggaca taatacaagg
     5821 catttttcat catgccgttg ttctttttgt tcatagttgc cctccctaag cgtcaattta
     5881 atttaacagt tttttatcaa tcctgaccat tctattgtac cataatttaa ttgagataca
     5941 gcattaattt gattgataaa cactaggttt caatacaccg atgtacggaa ggttacgata
     6001 tgcttctgca taatccaagc cataaccgac cacaaattca tttggtacgt tgaagccaac
     6061 ataatctgct tcgatattta ctactcttcc ttcaggtttg tcaagtagtg taacaatttt
     6121 gactgatttt gctttgcggt atttgaaaag atccactaaa taagcaagtg ttcttccgct
     6181 gtcaatgata tcttcaacga tcaacagatc gcgaccttct acattcgtat ctaagtcttt
     6241 gacgattttt acttcccctg acgagactgt tgcattcccg tagcttgaaa catccatgaa
     6301 atccaattcc aaatacgtat cgatagaacg aacgatatct gccatgaatg gtaccgctcc
     6361 ttttaagaca cctactacta ggggattggt gttttggtaa atttctgtta actctttgcc
     6421 tagttctttg caacgaactt gtatctcttc ttgtgagatc aaaattctct caatatcctt
     6481 ttccaacatc aggttctcct tccaattgct tctttttgat atttgtaaag aagtatgtag
     6541 tgtattttat cagtttcttc acgaatactc aaatatgaaa aggcaaaagg tatcaagcta
     6601 tatatatttc ttttggcatc tgtaatgatc caagcatgct gcctttgtga attaggtact
     6661 ttctgatcga taaaatatct agatactttt tttgtgagat tctctgttaa tttaatgcgg
     6721 tctcctgctt ttcttttttc gacatacagt tgcacatcag caggaatcca aatgtcatga
     6781 gaaaatagag accatgaatc tgttgctttg atgtcccctg cattttcagg tttgaccaga
     6841 ccaatccatt ctgtttcatt caaatatatt ccttgttctg gaatcagcaa caagcgctgg
     6901 tcttctgtct ggctagttgc ttgtttttct tttttagaga gtacaacaaa atgatactct
     6961 cttttcagta tccaattatt gccaatactg agttgccatt ggcttttttt ctgttgccac
     7021 tggtctaaaa tcgactccat ttgttgttct ttcaaaataa ttcctgtttt tgagaatgct
     7081 ttgtcgaaaa aggagtaaaa aaaatagtat cgttcagcat ctgtcatttc ttcaatcatt
     7141 tctgcagaaa atcgaaattg ttcctcccac tgttcgactt ccttttcgat caactgtccc
     7201 atatattttt gaatgacttg gtctgcccat tggatctgtt gcgaaaattg ctggaaatgt
     7261 ctcaacgcct gtgtattttc ttgtttcaat tgaggaacta ccaaatgacg aagccgattt
     7321 ctctggatat ctaacaattg gtttgtctga tcctcaaagt aaacaagctg cgattccgct
     7381 gcataagtat ataggtcttc tttgctaaaa ctcagcaacg gacgaatcag tctgcccgta
     7441 gcaaatggtt gtacttcttt gattccagaa aaagtattta actgtccccc acgaacgatt
     7501 ttcattaaca ccgtctcgat ttgatcatct ccgtgatgag cagtcatcaa cgtgtcatat
     7561 tgtccttctt ccatcacgcg agtgaattct tgataacgaa agttacgagc ttctgtttct
     7621 atggcggatt tggctggatc ttcccaaata cttacatata aagggagatt cttctcttca
     7681 caatatgagc gaagataggc agcttcttct gctgattctt ctcttaattg gtgattgatg
     7741 tggacgatac ctaaagaaaa accgatttgg cgttgcactt gtaaaagtaa atggagcaaa
     7801 accatcgaat caacaccacc tgataccgct gctaaaattc tcgatccttg ttgccagaat
     7861 ccttgttcat tcccgtgccg aataaatgac tgctgtaata gcggtgcttt tttcccgcct
     7921 ttttcaacgt tcatcctttc cacacccttt cttcctaaaa aaaagagagc gggaaaacgt
     7981 ctaaaacgac aattaacctg ccctctgttg atactatgac ccaaatagtc gcactatcat
     8041 tatttgcggc tttttagctg cgacgtccgc cacggccacc gcgctttcct tctgtgtttc
     8101 gcttcaatga agataagcgg tcatcgctat ctttcaaaaa tgaactcatc aatgaatcga
     8161 agtcttcttt ttgagctgaa ggttgactgc gagaaaaaga ttttttctgt gctggacgat
     8221 ttcggttgtc acggggatct ttttgttgat attttttagg acggctttct tctccagatt
     8281 cagcaggttg ttcctgcgct ttccggatag atagtccgat ttttccatca tcatttactg
     8341 aaataacttt aactgttact tcatctccta cacttaaaac atcatggatg tcttttacat
     8401 agccatttga cacttcgcta atatgaacta gacccgtttt tccagcacct agatcaataa
     8461 acgcaccaaa atttgtgatt cctgatactt ttccttgcag ttttgctcct acctcgattg
     8521 acataaaaaa aatgttcctc ctaattattt ccctttttta tattcctgct tattttgcag
     8581 ttgtagaact agttgattga tctgtttcat ttgtcgagct gtctgtattt gattctaata
     8641 taggataaac tttttcaccg tcttttgagt aaaagaattt gcttctggca agtttagcta
     8701 catactcatc atctttcagc aatgaaacat cttggtgcag ctgatcaacg tctgcgttga
     8761 cttctttcat ttcagtattt gcttcgactc tcaattcgtt caattgatgc atcctttgca
     8821 tgtcatggaa aatctgcaca cctattatcg cgaaaatgac caaagcacca acaaaaattg
     8881 cagccagtct gcgtcttcga aaaatcagtt gacgttgttg ttgctgaaat tcagcatact
     8941 ttttctttgc atattccgta tctagcgcag cgattttatt gctttttttc gtcacttgga
     9001 ttcgctccaa tctttttgat tgtctttttc attataccaa tatcgtgaaa gcttgtctaa
     9061 tctatttctc tagtgtttct gtgcgatttt cagaaataat ttcgtacatt ttttgtgcat
     9121 cttctttttt ggttgattca tgtaattccg taattttcac ttccaacgtt ttgttgccaa
     9181 actggacttt caaaatatcg cctactttga catcactgga agatttcgcc aatttaccgt
     9241 tgatttgaat tcttccttta tcagctactt cttttgctac tgatcgtctt ttgatgattc
     9301 gggaaatttt taaaaattta tctaatcgca tatcttattc ctcacttttt aattttttta
     9361 tttaatcgca ggattttctt accaaaaggc aacattagcc attctcgtac tgtaaacaga
     9421 cgaagacgaa tcactgcata aatgaacaca gaagccccga tcccaactcc tatcacgctg
     9481 acaaaaaaag cagtggaacg atgagttaca ggaccaaata ctatattcaa tacaccataa
     9541 tacagcagca gtactactgt catacaaaga aggctgaaca tcaacttccg tccaaaatgc
     9601 cgttctttcc aaaaagcatt tagctctttt gtttcaattc gaacaaaata ccaaagcgtc
     9661 acacctaatc ctaaaagggt agacaggctt gcacctgctg ttcctaataa accggtcaga
     9721 aaagaagtag tcaatccttt taccaatagt ccccatcctg cgccttttaa agaagggcga
     9781 aacgagttct tgctttgagc aatactttga taagcttgga tgactgcagt gaaagcaatg
     9841 gaaaaaacaa ataatacaag cgctgcattt cccgcataat ctttaaacaa cgcataattg
     9901 atataaggga gcagcaaggc taatcccaaa gacgcagcta atgctaaagc ggtcgtcaat
     9961 ctaagataga ttttggctgt ctgtaaaaac tgacgataga tccggttggt caaatgacga
    10021 gtcaacgcag gcaggaatgt cgcacttaat gcagtggcaa tcactaaacc taattgtact
    10081 aaaggctgac ctcgatcata gacccctttt gctatcttcg caccgtattc tgacagtccg
    10141 taaacttcca aagcattttt tacaaagaaa gagtcgatca gctggaaaaa gataagcaat
    10201 ccactataaa tcgaaaccag tccgccttct accagaaaac gccggatcaa gggctttgac
    10261 ggctgtttga acaatggaaa ccgtcggaag cttaacgctc ctccatgaat cttttgttca
    10321 tagtagtgaa ggatcccata tgcgcataaa ccgccaaaaa cagctccact cattgctgcc
    10381 gtccctgttt ggtacaccgt ccatccaagc tttctaaaag caaacgcact acagacaatc
    10441 acaaaaacac gaacgaactg ctcaactact tgagaaacag cagttggttc catcaagaat
    10501 cttccttgga aattccctcg ataaaaagat agaccaggca tcagcaaaaa ggtaaaggaa
    10561 actacttgga tcagcggttt caattgtatg tctcccatca tccaagcaat ccagctgcta
    10621 aatgcaaaga caaacaacca caaaaagaca cctgtccaaa atactaaggg ataaagattt
    10681 tgcaaagctt gctttcgctc gaaaggattc tttctttctg caacgtactt tgatatgaac
    10741 tgcggaagac cggataaagc taaagtcatt gctatcccat aaattggata aacttgctga
    10801 taaacataaa aaccttcgtc gcctactaag ttttggaggg gaattcggta aagggcactt
    10861 aatacttttg ctatgaaaga agcaatcgtc aatacaaatg ctccttgcat gacgcgccgc
    10921 atttctttat gatccaaatc attgctcctt cctacgctga cagatatttt tgctcacgca
    10981 acgctttgac aaacttttgg atttcctgaa gccatactgc ttcttccatt tggttgggga
    11041 tcgtcaagcg tatcaccatc tgttcttttt caacgcctag actggctttt agttttgtcg
    11101 ctgtcagtgc ttcgaataac tgttccacac tatagcgttt tgttccaact ttacttagag
    11161 taaagactat atgcgcctcc tgtttcctta tcgtttctag gagcgctcga tcgccatcca
    11221 ttttgatctg cccaattgtc aagagatgtg ctacttcttc tggatattct ccaaaacggt
    11281 cgagcagatc atcttctagt tcatctagca tttcttgcga atcaagttca cgaattcgtt
    11341 tatagatctc gattttctgc cgttgatctg ccacgtatgt ttcaggtaga tacgcatcga
    11401 caccgaggtc gatctcaaca gtggtcttct ccacttgact attctttcct tgtttacgag
    11461 aaacagcttc gcttagcatt tgagaataca tatcgaaacc gactgcatca ataaatccat
    11521 gctgctgagc acctaagaga ttacctgcac cgcgaatgga aagatcccgc atcgcaatct
    11581 tgaatccaga tcccagttct gtaaaatctt tgattgcctg aagacgtttt tcactgactt
    11641 cgttaaggat cttctgctgc tcatacataa agtaagcata tgcgacacgg ctgcttcttc
    11701 ctacccgccc tcgcaattgg tataacgtgg ataatcccat ataatcagcg ttctcaacga
    11761 acaacgtatt tgcgttcggg atatctactc ctgtttcaat gatggttgta gtgacaagaa
    11821 catcatattg accttcaata aattcaaaaa gcgtattttc taattgcgcc tctgtcattt
    11881 gcccatgagc ataaccgatc cgtgcttccg gaacgagttc ttggatttct tctacttttt
    11941 gttcgatcgt ttctacccga ttatataaat agaatacttg tccacctcgt cccatttctc
    12001 gatggattgc ttcgcgaata gccccaggat ttttttccat aacatacgtt tggatcggat
    12061 aacgattcgc aggcggtgtt tcgattactg aaagatcccg cacgcctaac atcgacatat
    12121 gcaaagttct cggaatcggt gttgcagtca gagtcaaaac atccacttgt gcacgtaact
    12181 gtttcaatcg ttccttatgt tttacaccaa aacgttgttc ttcgtcaatg atcagtagac
    12241 ccaaatcgct aaattcaata tcttttgaca aaattcggtg ggttcctaca acaatatcaa
    12301 cttgacctgt acgcagttgt tcgatggttt ctttttgttg ctttcttgtt ctgaaacgac
    12361 ttaatagccc gatatttacg gggaaacctt caaatctttc cagcatcgtt tcatagtgct
    12421 gctgtgccaa gatcgttgtt ggaactaaaa aggctacttg tttgctttcc tttatcgctt
    12481 taaaggccgc tcgaagcgct acttctgttt tcccataacc aacatcacct acgagtagtc
    12541 gatccattgg tttttctttt tccatatccc gtttgatctc cgctgcactt ctcaactggt
    12601 catcagtttc agagtagggg aaggcatttt cgaattcttt ttggtaggcg tcatcaggtc
    12661 caaaagcata tcctttttct gactctcttg cggcatataa tttgattaga tcgtcagcga
    12721 tatcctcgat ttttgaagag acttttcgct tggttttcgt ccattcactt ccgcctagct
    12781 tattgatgcg aggcgctttg gcttcagagg cgacatattt ttggatcaga ttcagttgag
    12841 taacaggaat aaacagtttg tcgtcgtttt gatacaaaat agtcatatag tcttggtgaa
    12901 cgccatctac ttctagcgtt tccatcccaa tgtatttccc gataccatga ttggcatgga
    12961 caacgtaatc tcctgctttt aactcattat agcttttcaa tcgctctgcg tttgttactg
    13021 tttgacgtct cgtttgtttt ttcgtcgttt tttggaaaat ttctttttct gtgatagcta
    13081 ctattttttc ttgaggaagt tcaaaaccag cctgcaaagc tccttcaact aattgtacct
    13141 gtcctctgat caatgcattt ttttctgttt ctactactaa tatgtcttca tctcgaagca
    13201 tttgttctgc tttgcgtatt ctgtcctttg tcggaataaa gatcacaaca gtctgttttt
    13261 gcttgcgcca acggtccatt tctgttttca gcaatggcat ttgtccgaag aactgttgca
    13321 tcgtccgata ttgaaaatta tggattgctt ggaaacgaag atttcccatc cctttttgga
    13381 agagtgagaa aaaggttgtg gtgaactttt ccttctgcac gattttttgt atatctgctg
    13441 aaaatgtctg ctcagaaaac acccgcaatt cgctgatttt ttgagtctgc cattctgccg
    13501 cttccctttc gatttcccga tttgtttcca tgattcgctg ataatcatca acgactagta
    13561 aactatttga agggaaataa tccaagatcg tcacagattc ttggtaaaga agatctgagt
    13621 agaattttgc ttcttctgtc gggacacctg cttgccagct tgaagtcagc tgaccgaagt
    13681 aatcttgcaa aaactcgcgg tctgcttctt ctgtggtcgc agcaagccgt ttttctagca
    13741 ttttttgcat ttgctctatt ccatgttcta gatcctctgg tccgtagact tgttcactcg
    13801 ttggggaaac ccagatttct tccgtttttt cgattgagcg ttgtgtttct gcttcaaaat
    13861 accgcataga atctatctct acatcaaata gttctacacg aacaggatag gttgtcgtta
    13921 acgggtaaat atcgataatg cttcctcgaa tactgaattc tcccggtttt gcgaccatct
    13981 cttctcgtac atagcccatc aaagtcaatt ggcgaggaag attttccacg tctatctcat
    14041 caccaatatg ccaattcaat tgcgcctgta gccaagtttt cttattcggc agtcgtttgt
    14101 gcaaagatgc gactggcaac aaatagatac ctgctttttt ttcttgaagt gcgttaagcg
    14161 ttgcaactct ctcgcctcgc gcttctggag aagaaaaggc catttcagca gaaagcactt
    14221 catccactgg aaataaatgg atctgatctt gcggaatcac atgttgcaga tcttctgcta
    14281 actgattcgc ataatacaaa tttggcatca gaataatcat gtttttattt gttttcttaa
    14341 acgctcgagc aaatagaagt gttttcgctg agcccgctaa tcctgtgatt agttgtctag
    14401 gcattttttc ttctaaattg ctgacccatt cttttacttg cggatctttt ccgactaatt
    14461 gaataatatc cattcctttt cctccttcct caattgaatc gattcatcgc atccacgaac
    14521 gaatgccctt ctatgagata ttcgactgct ttaacagatt gacacatgct ttcttctatc
    14581 aaaggctgat tttctttgct gaaaggacta agcacatgct ggactactgt ttttttccct
    14641 tctggcctgc cgattcccac cttgattcga tcaaagacat tcgtattcaa atgggaaata
    14701 atacttttga taccgttatg cccgccggca cttccctttt gacggagccg gatttttccc
    14761 actgccagat ccaaatcatc gtagattacg actaattcct ctggataaat cccaaaataa
    14821 gtcattagtg ggccgactgc acgaccagat tcattcatga atgtttgtgg tttgactaaa
    14881 aggatctttt ctccattatg aaaaaattcg gcaacttctg cttcgaacgg attttttttg
    14941 aaagtagctt gatgctcgcg tgcaatttta tccacgacca taaagccgat attgtgtttt
    15001 gtatattggt atttggtacc tgggtttcct aacccgacga tcattttcat ttttgttttc
    15061 cacctaattc taaaaagcag cacaaaaggc tggactgcta attagcggtc cagtccatcg
    15121 agcttttttc ttttttatac ttgtcaatgc atagtataac acaaaacaag gcgtttcggc
    15181 taaaagagaa tcgttaataa atgataaaga aattaaaaat ttcttcaaag tttaatgaaa
    15241 taattgatat ataaaaatag tgaaataacg cacatgtaag cttttacata taatacaacc
    15301 ttgttatctg ttatatttta tagccaaata gcgtgacatg gggcttgaga aatgttagca
    15361 taagcatggg aaaaaacaaa tcaatggaag gattgatact aaatgactgc agctgcagaa
    15421 aaaaaagatc atcaaaaagt tattcttgtt ggagacggcg ccgtaggttc tagctatgcc
    15481 tttgctctag ttactcaaaa tatcgctcaa gaagtaggta ttgtggatat caatactgct
    15541 aaaactgaag gagacgcaat tgacttgtct cacgctttgg catttacttc acctaaaaaa
    15601 atctattctg ctacttacgc agacgcacat gatgctgact tagttgttat tacagctggc
    15661 gcaccacaaa aaccaggtga aactcgttta gacttagtaa acaaaaacct aaaaatcaac
    15721 cgcgatgttg tcacacaaat cgttgaatca ggatttaacg gtatcttttt agttgccgct
    15781 aacccagtag atattttgac ttactcaact tggaaattct caggtttccc taaagaacgc
    15841 gtaatcggtt caggtacctc tcttgactca gctcgtttcc gtcaagcact tgcggaatta
    15901 gttgacgttg atgcacgtaa cgtccatgct tacatcttag gtgaacacgg agattcagaa
    15961 ttcccagttt ggtcacatgc aaatgttgcc ggcttgcaaa tctatgaatg ggtaaaaaac
    16021 aacccagaaa tcgatgaaga agctatggtt aacctattct ttggcgtacg tgacgctgct
    16081 tacacaatta tcgagaaaaa aggcgctact ttctatggta tcgcagtagc attagcacgt
    16141 atcactcgtg ccatcttaga tgatgaaaat gctgttcttc ctttatcagt attcatgaat
    16201 ggcgaatatg gattgaacga catctacatt ggtgcacctg ctgttatcaa ccgtcaaggt
    16261 atccaaaaag taatcgaaat tcctttaagt gacagcgagc aagaccgtat ggctgcttct
    16321 gctaagcaat taaaagaaat tctagatgaa gcttttgcta aactagacgc tgaataatct
    16381 ttgaacatga cggaaaacat ctattcggtg ttttccgttt ttttatttta tcacccttat
    16441 ggtttctaaa ttttttcata aagtagagat aaccttcgtc ttttcctcta aattatgcta
    16501 tcatgagacc gttccaccct aaacttttac ttaaggagtt gtcgatatga caagatcttc
    16561 gcatcgaaaa aaatcgaaac atgttgcttt gctttcagtt cttggtgttc tcttagtgct
    16621 gatcagcttt tatacctatc gcagtacata ctacacaaag cattttttgc cgaatacaga
    16681 aataaatgga ataaatgtca gcaatctaac agtcgaaaaa gcaaatgaaa aattaaaaga
    16741 agcttattct gataaaaaac tggctattaa agaagatgga aaagtctggc aggaagtacc
    16801 aaaatccgag ctcggctaca aagatgattt cacttcggag ctttctccga tcctaaatga
    16861 acaaaacgga tggacttggg gaatgactta tgtctcagcg gcagaaaaac aagaaataga
    16921 tcctgtcagt caaaaccacc aaaaactaga cactaccgtt cagacattga caactaagct
    16981 gactgatttg aacaaagacc ggacacctac tactgatgca acgatcgaaa aagcaggtga
    17041 ctctttcgtt atcaagcctg aagtcaatgg aaatacgatc gacgtcgatg ctgcggtcaa
    17101 acaattaaaa agtgcggtaa actctggtaa agatacaatt gaattgactg aattcaaaga
    17161 aaaaccaaaa gtaacatctg aagacagctc actgaaagaa caactcgcat ccatgaatgc
    17221 tatcgcaaat gtaaaagcta cttacagtat aaacggtgag actttccaga tcccatcaag
    17281 tgatattatg agttggctga cctacaatga tggaaaagtc gatttagata cagaacaagt
    17341 acgtcaatat gtaacagatt taggcacaaa gtataacaca agtacgaatg acacaaaatt
    17401 taaaagtaca aaaagaggag aagttacggt cccagttggc acttacagtt ggacgatcca
    17461 aacagacagt gagacagaag cattgaaaaa agcgattctg gcaggccaag attttactcg
    17521 ttcacccatt gtgcaaggtg gcacaaccgc tgatcatcca ttgatcgaag atacgtatat
    17581 cgaagttgat ctggagaacc agcatatgtg gtattacaaa gacggaaaag tggcccttga
    17641 aacagatatc gtttccggaa agccaactac acctacacct gctggagtct tctatgtttg
    17701 gaataaagaa gaagatgcaa ctttaaaagg aacaaatgac gatggaacac cttacgagag
    17761 tccggtaaac tactggatgc cgattgactg gacaggtgtt ggtatccacg attcagactg
    17821 gcaacctgaa tacggtggag acttatggaa aacccgcggt tcacacggct gcatcaacac
    17881 cccaccaagt gtaatgaagg aattattcgg gatggtcgaa aaaggaacgc ctgtattggt
    17941 cttctaacaa aaataatata taaaaagaaa gcacccgtac gattggtaca ggtgctttct
    18001 ttttattatc taacaatgtg ctacttacat ttcagcagca gtaaagactt ctgaaacatc
    18061 atcatcgtct tctagcttat ctactagacg ttccaattgc tctttttgtt cctcatttaa
    18121 atcaatcgtc gtttgtggaa tcatcgtcaa ttctgcttgc gccaaagtga actctttttc
    18181 caactcatct cgtacagcag caaaatcttc tggtgcagta taaatctcga atacttcagg
    18241 agaagtttgc agatcttctg cccccgcctc taatacatcc tcaaacatcg tctcttcatc
    18301 aacagtcaac ccatcgcgtt caatagcgat atatcctttg cggtcaaaca agtaactgac
    18361 tgaacccgtt tctcccagtg atccaccgtt tcgagtaaaa gctactcgga catttgtcgc
    18421 tgtccgatta cggttatctg tcaatgcatg gactaagatt cctactccac ccggaccata
    18481 tccttcatac gtgatttcat cgtaattcgt ttcatctacg gcactcgtag cctttttaat
    18541 cgcccgttcc acattgtcgt taggcatatt tgcagctttt gctttgtcca caacaagccg
    18601 caaagctgga ttcatagatg gatcaggacc gcctgcttta gctgccatat aaatttcacg
    18661 agaaagtttc tgaaagactt tccctcgttt tgcatcttgg gcattttttc ttccttggat
    18721 attatgccat ttactatgtc ctgacatgat ttttcctccc ttgttctttt gagaatacac
    18781 ttctattcta gtactatttc acaaaaaaac aaagccacag ccaatctata aacgcttctt
    18841 gaatgccgca aaaagcacac ataatatcac agcagtcaga gcaccgatcg aatccaacat
    18901 gacatcttgg aacataggtg ttcgtccacc ggtcatcatc tgatggaatt cgtccaaacc
    18961 tgcgtaccct gttgctgaaa gccaagcaag gatcgctgtc agccaccaaa tttttagctt
    19021 ggggaaaaca cctaaaaaca agctgcctcc caaaataaaa taggtaccga aatgtgcgcc
    19081 cttccttaaa aagaactcta aaaatttggc gtagcccaga tggtcgatac tcaccatact
    19141 tccaccataa ttaaaacgta tacccgctaa tgtgtcttta aatggctgat taggcagcca
    19201 gctttctaat cgcgaaactt gtgattgctg tccgtaagtt tgagaagaac tgtaaaataa
    19261 gattataacg actatcagtg cgattactaa ataaatattt ccgtttttca tttgtt
//
LOCUS       DAIGOK010000050        19187 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.050, whole genome shotgun sequence.
ACCESSION   DAIGOK010000050 DAIGOK010000000
VERSION     DAIGOK010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.050"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(171..359)
                     /locus_tag="NOA01_001388"
     CDS             complement(171..359)
                     /locus_tag="NOA01_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632590.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKE"
     gene            complement(379..801)
                     /locus_tag="NOA01_001389"
     CDS             complement(379..801)
                     /locus_tag="NOA01_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HCI0632591.1"
                     /translation="MESVYRQERKSNRKKGQTMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGVLTFNFSDPEGNYFVIGKAESSD"
     gene            complement(946..1656)
                     /locus_tag="NOA01_001390"
     CDS             complement(946..1656)
                     /locus_tag="NOA01_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632592.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(1674..2171)
                     /locus_tag="NOA01_001391"
     CDS             complement(1674..2171)
                     /locus_tag="NOA01_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCI0632593.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPKHLADYLTQAYAPEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(2208..2660)
                     /locus_tag="NOA01_001392"
     CDS             complement(2208..2660)
                     /locus_tag="NOA01_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCI0632594.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(2795..3400)
                     /locus_tag="NOA01_001393"
     CDS             complement(2795..3400)
                     /locus_tag="NOA01_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCI0632595.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(3955..4590)
                     /locus_tag="NOA01_001394"
     CDS             complement(3955..4590)
                     /locus_tag="NOA01_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCI0632596.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKKSMTISNKEMA"
     gene            complement(4592..5461)
                     /locus_tag="NOA01_001395"
     CDS             complement(4592..5461)
                     /locus_tag="NOA01_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HCI0632597.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYISMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(5484..5891)
                     /locus_tag="NOA01_001396"
     CDS             complement(5484..5891)
                     /locus_tag="NOA01_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCI0632598.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDRHKLAELYEQVGLKFSELYENDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRSD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(5910..7082)
                     /locus_tag="NOA01_001397"
     CDS             complement(5910..7082)
                     /locus_tag="NOA01_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCI0632599.1"
                     /translation="MSVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAILWMATMTTVFGY
                     FAGLRGIVLILVGGFIGACFAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVA
                     KLFGEKGENKKSIEDIKLPTHFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGD
                     QNYIMFAFLQAIQFVVGVYVLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFP
                     YSPNAVILGFITTTIGTIIAMFTLPMFGLAMILSGMLTNFFAGGTAGIFGNAVGGRRG
                     AIIGGIAHGFFITLLPALLVTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(7109..7411)
                     /locus_tag="NOA01_001398"
     CDS             complement(7109..7411)
                     /locus_tag="NOA01_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCI0632600.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(7436..9522)
                     /locus_tag="NOA01_001399"
                     /pseudo
     CDS             complement(7436..9522)
                     /locus_tag="NOA01_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
     gene            complement(10011..10776)
                     /locus_tag="NOA01_001400"
                     /pseudo
     CDS             complement(10011..10776)
                     /locus_tag="NOA01_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
     gene            complement(10887..11756)
                     /gene="mdcB"
                     /locus_tag="NOA01_001401"
                     /pseudo
     CDS             complement(10887..11756)
                     /gene="mdcB"
                     /locus_tag="NOA01_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
     gene            complement(11781..12865)
                     /locus_tag="NOA01_001402"
                     /pseudo
     CDS             complement(11781..12865)
                     /locus_tag="NOA01_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
     gene            complement(12889..13482)
                     /locus_tag="NOA01_001403"
     CDS             complement(12889..13482)
                     /locus_tag="NOA01_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HCI0632601.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWATEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(13486..15162)
                     /gene="mdcD"
                     /locus_tag="NOA01_001404"
     CDS             complement(13486..15162)
                     /gene="mdcD"
                     /locus_tag="NOA01_001404"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HCI0632602.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDFIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEETIVISIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKFRQAGYPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(15152..15451)
                     /locus_tag="NOA01_001405"
     CDS             complement(15152..15451)
                     /locus_tag="NOA01_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HCI0632603.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(15553..17199)
                     /gene="mdcA"
                     /locus_tag="NOA01_001406"
     CDS             complement(15553..17199)
                     /gene="mdcA"
                     /locus_tag="NOA01_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HCI0632604.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVVADKADKDGNL
                     YTGFNTEETPTIVEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADEPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALVVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLREAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(17206..18117)
                     /locus_tag="NOA01_001407"
     CDS             complement(17206..18117)
                     /locus_tag="NOA01_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCI0632605.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLTFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEMVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            18237..18527
                     /locus_tag="NOA01_001408"
     CDS             18237..18527
                     /locus_tag="NOA01_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCI0632606.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLYTLEEGINYCLRSMRRSLLPSYCI"
     gene            18479..19093
                     /locus_tag="NOA01_001409"
     CDS             18479..19093
                     /locus_tag="NOA01_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HCI0632607.1"
                     /translation="MSSVNEKIVTSVILYLEVASVSIPNLVRIFMEKHPDIQLTIMQH
                     HLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAIPKKHPLAKKEHLQLEDIQRTPIL
                     MLSKSNAFRHTLDAALEAKSIELTISSTTDDPATLRSIVKQGLAVSFFPKVSWSYDKN
                     DPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAKTMREFYLGK"
BASE COUNT         5318 a         4083 c         3312 g         6474 t
ORIGIN      
        1 aattcggatt ttaagcaaaa tccgaacgtt tcatctgatt aaatcccttt atctcaaagt
       61 tcatgaacat tcactttgga ttacaagaat ttctttattg tgttaataaa gtttcttcat
      121 tctagtctca aaaatcaata gtgtgatttg attttaccat ttcgtttctg ttattctttg
      181 atgaattctt ttgtatcgac aggattcaga ttatttagtc cgccaaaacc tgtgtcgacc
      241 atttttcccc agccttttgt cttctttttt cctgatttga gattctttgc tcgaagcttc
      301 tcactatgtt ttgctcgccg atacttcaaa taaccttcta atcttcccat tagatccact
      361 gtttttcctc ctttttattc aatcagacga ctcagctttt cctataacaa aataattgcc
      421 ttcagggtcc gaaaaattga aagtcaagac gccattgttg tctgtgattt ctcctggttc
      481 ttccatgttc tcgtagatag tctcaaattc gtccgtaaaa aacactaaag aaggcggtgg
      541 tcctaatact tctggtgaaa atttctgcac aaatactttt gggaatacgc ctaattctaa
      601 ttcttttgag atgctgagta caatactctt aaattcttct ggcaattcga ttgtttcaga
      661 aatttctgca ccaatttttt ctacaaagaa tcggcaaatc atctctacgt cattgacata
      721 aagcatcaca cgtgtttttt gtatcatcgt ttgacccttc tttctatttg attttctttc
      781 ctgtctataa actgattcca ttatatcacg aaagcgcaaa caccttgtgt ttgatactat
      841 tcaagaaaaa taaagcaata gtttctagga ttgctttcct ccacaaactt tttttacaaa
      901 aaaaaaaaaa cggcaatgaa tgccgctctt cttttttcga ttcttttata attctgcttc
      961 tgattcatct ttggttagtg tgtgtctaac actagttacg ccaaatgttt gatcgatttt
     1021 ttccttcaac gcttcgattg cctgtgcgcc acgtgccgct tgttcagcat tgatggattc
     1081 aatgaccagc accgctgctt gtgtttcttc cgtcagcatc gttctttggg cttcacgcca
     1141 gttgagacag cgacagatcg ctccttgttc atcatagtag atgatttctt ccggaagtgc
     1201 cggagcatcg gaatctgcac ctaaaggcag gaatgattcg ccaccttggg cttttcctaa
     1261 atgcagattt cctgcgatct tttcgatatc ttctccgccg caaggtacgc caaaagacaa
     1321 ggaaatacta ttatagatat ctacaagcgg attgattggg aaaaattccc gttcttgatt
     1381 tgcacgtttc agtaaagctt caatagaaga acgtgcacct tttttagtct tgaatgtggt
     1441 aaatgcctga cgccactgag caatgacttc gttttggctg aatgtttctt ctgtaaggta
     1501 atttcttgct tcttttgctg cctcattcaa taattgatga tacgtttctt cactttcttt
     1561 tgttacgtgg ttatcgatac catttacgga taagatttcg atggttgctt caggaaatac
     1621 ttcccagaaa ttggtatcca ctattacttt tttcatatgt tcacacctct tgattagaat
     1681 aattgcttga tgaatagata gtctgtttgg ggatcatctc ccatataaaa aacatgttcg
     1741 ctgtatttga taaatccttt tttacgataa aaagcgagcg caacatcgtt gtgttcccaa
     1801 acaccgagcc aaatggctgt tttttgcttt tcttttgctt tttcaatagc tgtatctagt
     1861 aaaaatgtac cgataccttg atgtttaaat ttttttcgta catagattcg ctcgatttct
     1921 aaaaaatgat cagccatttc ctcagattga ctcgttcctg tatttagttt caggtaaccc
     1981 gcaggctctt gatcgatact cactaggaaa aaatcggttt ctttatcttt tagctcttct
     2041 gtaagttttt ctggcgcata agcttgtgtc aagtaatcag ctaaatgctt cggtgaatta
     2101 tcttttccaa atgtatctgt aaatgtttcg atactgattt gttgtagtaa gtctacatct
     2161 gaaggcgtca tttttcgaat ttcgtattta ttcacggctt cttcctttca atagatcctt
     2221 ttattgcctt ttttgacaaa attccaatct tcgctaacat tcttgcatac tttatttaaa
     2281 agatattcca attgttttgc ttcttcgtca gaaagaccat ttaatgcgac tgcattggag
     2341 tagttgtttt ctcgaatgat gaaaggatag atttctgctc cttttttagt cggatagagt
     2401 ttcttgattt ttttattttc tttatcttcc aaacgttcga tcatcccatt ggattctaat
     2461 ttcttgattg ctcgggcagt cgttgtacga tctactttga tcatttctgc tagtttttct
     2521 tggatgattc caggattttc gcaaatacgt actaaataaa gatactgtcc tcttgttaaa
     2581 tcgacttctt tgaattcgat attgctgata gaatctagcg ctcgggcaat cattccgatg
     2641 tctcgaagga tttcagccat ttcatcccac ctttattttg tttgtaagac tagtttacct
     2701 ctttttgttg caaatgcaac tattttcaat aaaaaaatca agttcttttt taataactag
     2761 taaagtatat tcttttcttc ttttacgaaa aagctcaatc acaatagatc tggtttttgt
     2821 tttctaaaat aagtaactgt ttcttcgttg ttcgcttttc tgatagcttt ggcaactctt
     2881 ctatactaaa aaatcccatg ttggaagttt cattattctc aataaacttg gcctcttcac
     2941 cgtggatcgc acaagcaaaa acaagtttat aatactgaaa aagctgtgga atatcttttt
     3001 gtttattcgt atcaaaaact gctcgcaact gagtagtttc tactgttaat cctgtttctt
     3061 catagacttc tttacgcaca ttttcttcag gagacaatcc tatttcagca aatccaccag
     3121 gaagtgacca ttctttcgtc cgtttatctt cgattagtag gaccttgcca tcttttttga
     3181 tataggcacg aacatctact tttggtgttg gatgtccttc tccaatcgtt gtgagcgctt
     3241 ttatttcttc gaaagtttct tttcctattt cactgatcag ttccaatgaa atcgtccgga
     3301 tctcctcaaa acgctcttta tcaaatacat cttttccata atatagacca gcttgagaaa
     3361 gtgcgagtag tcttttgtat tgagccaact tcttgttcat tctcttccct tcttttttag
     3421 caaatgtatg actatgtaat caagtataga agactagttt aattacggca aataaatata
     3481 atgaaatctg caataaataa aagaatgtta aaaaactcga gaacaaaaga cattcgactt
     3541 ctctggggct gtgaataaat ggcgttcaaa agcttgcatc tgcggtaata agccccacca
     3601 aacgaaaaat atgacgagct attcttgtct ggctgttctt attaattgag gcaggacagc
     3661 tttttcacaa cctccccact aaacggtgtt caatcagctg atgctcggta ttcactaaaa
     3721 aaacggcaaa atatgaggag ctattttgcc gtttttttct ataatactca tattaaaaca
     3781 ctgacttcac aacctcttcc ttaaacggtg ttctcctacc aactcctcag tcacaagtca
     3841 caatattcat caaacatgag aagctgtttc ttgaaattgc tccttgtgac tcggtgctga
     3901 acggtaggct cacgacctct cggtaaacgg cgttcaaaag ccaatttttc gatattaagc
     3961 catttctttg ttacttattg tcattgattt tttttctaat ttatccaata gtggagtgta
     4021 atccacttga gtatagtctg ttacatcaaa ttcatatagt gttcctaact ggaatttgtc
     4081 atattttcgg tcggcgatga tctttttgaa tgcttctttc gtaatatgat tggtcccttt
     4141 cgttcgatct tccaatacgt caccatagtg ataatgatcc atgatatagc tcaaatgacg
     4201 atctgattca cgatcacgtt tgtaccgacg ttgccataac acttcaaagt ctgctgttag
     4261 gcgaatcgtg atcacctgat atccgtatct atctgctaat tcttggagtc gtgctttttg
     4321 cttatcacta aaaggatact cggacacaat cactttcttt cctgcttcca tatacaaatc
     4381 aagtgctttg taatagaaag gataaacttt cgtcctttct tgttccgccc gttcttccaa
     4441 attgttaaac ccgatggatt ctgcatagta ttccttgatt tcatcgagcg tgatggcata
     4501 catatctgga aatcgttccc gtagtttatt cataagatat gtttttcctg taccaggact
     4561 accagccaga agaatgaaat actttttcat ctcatttatt tcctaagtta attggacttt
     4621 ccaagttcat aaacagtcca atcttatgct tgacaatttc ttttactgct tcatttgctg
     4681 gaatcacatt atggcgaaga gacttgttga tttctgtttc actaaaggtt tctttagcta
     4741 ctttcgtcca gttgactaaa agctccgttc caacattgaa tttgcggatc ccgttattga
     4801 tcaattcagg ataatcttct tctttcacac ctgttcctcc atggataact agggggacat
     4861 caactactgc atcgatttct ttcagcaagg gaatgtttac ttctgttttt gatttgaatt
     4921 gtccatgatt cgtaccgata gcaatagcta atgcatccac gcctgtagct tctacaaatt
     4981 tcactgcgtc ttccggtctt gtatatacct tgtcattttc agcgacatgg attccttctt
     5041 ctgtaccgcc gattgttcca agttctcctt ctacggaaac accgtgcgca tgggcatatt
     5101 ctactactgc ttttgtcttc aggatatttt ctttgaaagg cagatgggac ccatcataca
     5161 tgacagaagt atatccgctg tcgattgctt ctttgatatc atcaaagtct cgggcgtggt
     5221 ctaagtgcaa gactgcatca accatttctt cttctgcaat agctgtcatt acattgacta
     5281 acacatgcat gctgatatat ttagcagtat cgacacttgt ttgaataatg attggcgaac
     5341 ccatttcttt tgctgcgcct agcatggcag gaagcatttc taggttatgc gtattaaaag
     5401 ctccaactgt aaaattcaac tcttctgctg tttttgttac ttctttcaat gttgtataca
     5461 tcttttattg ccaccttatc tttttatttg tttcctttga tcgttacttg ttttgcaatt
     5521 tgtcgcatct catccatttt gcccatgtag taatcgatcc cttgatcatc accagcacga
     5581 gcagcatctg aacgcttctg gttatagagg ctcatcagtt ttttataccc atctccgatc
     5641 gtctgtttat tttccaagct cttttctaaa tattggatgg cttgatcgtt ctcatacaat
     5701 tcagaaaatt taagtccaac ttgttcataa agttccgcta atttatgcct atcttgggta
     5761 gtggaaatct cttgtttcaa ttgtgtaatc tctgattgaa tcttttcggt cgcttctggc
     5821 gatagggctt tttcttcttt tgccagaact tttttcggtt ctttttcttt tttcttaaaa
     5881 aatccaaaca tcattttccc tactttcaat taaaatgctt tcaaaactgg gcttaagatc
     5941 caggcatata agagtgctgc ggctactgta tctacatcgg ttgctgtagc attgatgaat
     6001 cccatcgaat taaagatcgt tactagcaaa gcgggcaata atgtaataaa gaaaccatga
     6061 gcaataccgc caatgatcgc tcctcggcgt ccgccaactg cgttgccaaa tatccctgct
     6121 gtccctccgg caaagaaatt ggttagcata ccagataaaa tcatcgctaa gccgaacatg
     6181 ggcaaagtaa acatggcaat aatcgtacca atcgttgtcg taatgaaacc taaaatgaca
     6241 gcatttgggc tataaggaaa gaaaactgga caatctaagg cagggatcgc atcaggaact
     6301 aatttcatag ctatccctcg aaatgccggc acgatttcac ctaacagtag acgtacacca
     6361 gctagcaata catacacacc cactacgaat tggatggctt gtaaaaaggc aaacatgata
     6421 tagttttgat ctcctgataa ttcagaagcg aatggttcgc ctgcaaataa aacagtgatg
     6481 atatatagcg ggaccatcac gaccatgaca gacaaataag tatcttgcag aaattcgaaa
     6541 tgtgtcggca acttgatatc ttcgatcgac tttttatttt cgcctttctc accaaaaagc
     6601 ttagcgacac cggcttcaaa taagtacccg atcgtacaaa aatgacctaa agcaatatca
     6661 tttgatccag tgattttacg aatgatcggt tgcgctactg ccggcatagc aatggcgaaa
     6721 caagctccga taaaaccacc gaccaagatc aatacgatcc ctcgcagacc ggcaaaatag
     6781 ccaaagactg tcgtcatcgt tgccatccat aaaattgctt gaccattgat agattcaata
     6841 gaagggatga tcccttgcat atgaaatcct tcagtaaaga ttttcccgaa ataagttaaa
     6901 cttccaacga tgatgctcga accggcactc aacacttgaa atcctaggag tgtctttaac
     6961 gtgccagaga tgacttgtcc tgctgatttc ttctgcagta gtagacccaa catagcaatc
     7021 aatgcaatgg tgatcgatgc ctgagtcaaa atattttcga tgataaaatt gataacgctc
     7081 atgtttttac cctcttttct ttttgtattt agataactcc cttatcttgc agaactgggg
     7141 tgattttttc ttcaatttca ttttttgaaa caatattttt caaatagatc atcgccgttt
     7201 taggatcaac tgcgaacttt tcgaactgcg aacgaaaatt ttctgctgtg atgatcacat
     7261 ctggtttcat ggaaactgcg gaagaaatat cacaatggtc cagtttggct tctactccta
     7321 atttgttcaa aacatcctca gcagacatct gtgctgcaaa actacttcct aaacctgcgc
     7381 cgcatacgaa taaaatgttc aatttgttca tctttgttct cccccgtatc atttattatt
     7441 tctcaactac tgaaaaaagc agttgtttga aatcttcgat atttgtgcat tcaatcaatc
     7501 gatccaactt ctcttcgttg ttgatcaatt caattagttc tttcatgatg ttcagatgcg
     7561 agtaggagtc tactgctgcc aaacaaaaaa taatctttac tggatccatt tcaggattac
     7621 cgaaatcgat tggttgctca atcgtagcta cgctgactcc taatttattg accccatctt
     7681 ctggtcgggc atgggctagt gccatgtgtt tgccaatcac gatatacggt ccgtaatcat
     7741 tcactgcacg aatcatcgct tcgacatagc gctcttcgac aatgttttct ttaaccaatg
     7801 gtctagcagc tagttcaatc gattcctccc aagtagtggc tttttctttg atcatgatat
     7861 tgctgtccgt caaaatatct tttaacatag gctggatctc ccttttgttg atttctaaat
     7921 tgtttgtttc gaataatttt tctaacttgc catagatgat tcgatctact cggccaccac
     7981 tttctttcat gacatctatt atttgagtga ataactccgt cgattcatga ctgttaggct
     8041 ctaaccgctg tagttcttca tttcttttca aaaagtcttc tataaccaag atattcgtct
     8101 cagttaaaat cggatcgaca actaaaagcg gcttatttcg ataattcaat tgaaaggtgc
     8161 tgaaaaccag attgacatcc aatttatcta ctaattcgat ttcttttgaa cttaaaactg
     8221 cagtaatctc gatctgagga aacttttctt tcagattctc tgctaacaga tttccagtag
     8281 ccatcccatg gttacagaca acgactgatt tataaacata attcaagtct cgcttgatta
     8341 cactggcgat cgtcaaaaag tggatgacaa gaaaagctat ctcgtcctcc agtatcttgc
     8401 caccaattac ttcttcgatc gtaggaacaa aatgagtgac tgcgcggtag attgggaaat
     8461 aatttgttcg gatgttttct ttcaatggat tcatgacttg gatatgattc ttacaatcag
     8521 tgcagccatg tgcttataga gattttcgaa taaagcttct tcctttagat ggaaaggtac
     8581 gtgtgtctcg ttctctacaa attggatcaa ttggatcgtt agtaattgag cttgtaccca
     8641 ctcgattgaa ttggagatat ctctagcatt gaacgtctca atagtgaaac gaagataatt
     8701 tctttctact tgcgggacat tgtccaattg aaattcctcg atcatgtggt caataaatgc
     8761 agaaaagcta ctgtctttta tttctgtatt tttccaatta atggcagaga taaattcatg
     8821 tttcctgatt cgctggatcc agatcaatgt aaaaagcagg atttgttttt tataaatatc
     8881 atcttcttgt ttcaacaact tattggaata gatctcctgt atttttttga tttccaccaa
     8941 aggaaaataa tggagaaaga ttttttgagc cggtgttaat tgtgcatctt gattcataaa
     9001 gaagtcggtc attcccagat tctgattgat aaaatcatag atcatcgtac ggatagatcg
     9061 ttctgaacct ttataaacca gtccttgctt tccataactg acgatctcta tcccataact
     9121 gaccagtgtt gctcgtaacc gtctcatatc ttcatctaat gtacttttag agatttgaaa
     9181 ttcttcttct tttttgttta gatggatcgg gattcggctg taagcaacgg ctaaaactag
     9241 atcaaaaata cgttcgcgcc gattcaaaaa gccgtctttc acatctttta tttcagtttc
     9301 taatcgagct tgcttttcct tttccagctt caacaaaaaa cctttgttgc gaattgtacg
     9361 gatctccgga agctttttac tgctcaacca gatattcgct tcaagaactt cctgacggat
     9421 cgtccgctga ctgacattga actcacttgc tagatgtttt gcttttactg gatattgggc
     9481 tgtcatgatt ctgtgaagta tttcaattgt tcttgttttc atagaaatga tctccttctg
     9541 atttcaagta tatggttccg cttacataat aaaaagtaga tatttctgcc acttgaagtg
     9601 gaaagaaagt aagcaaagca aacaaaaaat aagccatatg ctttttcatc tcctcaacga
     9661 aaaagacata cgcctattat tacaaaaatt ttgcccatcc atacaaaaaa gaggttgtga
     9721 aaaagctgtc ctgcatcaag taataagaac aactaaacta aaatagtgct tcctattttt
     9781 tgtttggttg ttcttattat cgcagatgca agcttttgaa caccatttag tggagaagtt
     9841 gtgacagaga tgttcagcct tgagcgataa gaagcgattt tgaaaaatag cttcccatat
     9901 tttacaaaat cgtgccttat tgccgatagg ctatctctgg cacaccgttt attcagaaaa
     9961 aggagttgcg acaaggcttt tgtcacaact ccgttcataa atatttcctg tcaatccagc
    10021 ataatcggct gaataaaatc ggcttgccat cttttttctt tctcagaaaa atcaaaatac
    10081 catatctgag aaaaatactg tcgttgtgtc ttacttcctg agatcaatcg cccatctttt
    10141 cgatcgactg aataatcaca gcatctgaaa tcaattcgga tggcaaaact attttcgtgg
    10201 atctgtcgat aatttcctac ttcctgaata gttatttttt taggtatgat ttcttacacc
    10261 gtctgctttg tttttttgca gaactttctg attttcggca aacagcttgt ctgtataaca
    10321 gcttctggct ttttcaggag tttcttcttc ccaagcggat tggatcttca tgaatatctt
    10381 ttggatttcc agaaactgtg ctttcttttc tttagccgtt cctgacatgc gggacaatat
    10441 ctccgctttc agccttttct tttgtctttt attcttgacg atagaaaaag cagaaacccc
    10501 tcctactgac aaaacagcta atgcaagaaa tcccgtttgc tcataactag aaggacctgt
    10561 acttggacca taatacccat aagaatctct gccgtaagaa cgactgccaa aataactgcc
    10621 cccatatgaa cgaccgccaa atgaacctct gcccgaactt cgaccgctag atgtacctcc
    10681 accatgacca cccgcgacag cgaatccttg ggtcggcaga gcaaacaaac ataggcccac
    10741 taacaaaatg attgctaaat aattgatttt tttcattgtc ttcctcgtta tcttttatta
    10801 caaaaaacta tttttatcat agccttgcta aaaggaagag tcaataacca gatgcataaa
    10861 ctgttttttt agcaattttg tcttatttag tcattgagta acatcgaaca gatgtatgtg
    10921 tcggcagtcc ataattcctc tatcttcagc atgaacaagc ttgcagacaa gagatctgcg
    10981 cttcctccag gagagatttg gttttcctga caaaagtgca acagtgctgt aaatgaacga
    11041 tttggtaaag cactttgact agcagcatga gcaagttttt gcatttccac taacgttttc
    11101 taatcactgc gatgcaatac gcatgtgtca tctagcgaac tcatcaaaac taacaacata
    11161 tgcatttgtt tctgtacata tgaatcatta tctagctcct tagctgcttg gattgcaagc
    11221 ctgatatgcg gataccctaa tattgcttct tcatatgcac catttacgcc atatttttgc
    11281 ttcaccctat gaccatgagt gtttttttta tgacgatatt ttgtatcagg aaaagcagca
    11341 agcttgctga catcatcaag tattttcgat aaagaaaaag gctgttccat actgattttt
    11401 tgtgcaatga cactgatcaa tagccccatt gcccaaatcg ctcctttatg ggtattgatg
    11461 ccatttgtag caatcagcat tgccttttcc gcttttcgcc caatctcagc aattctttct
    11521 cttaactcct gatcaatagc atgatgccat gctatttgag ccatttcttc aaaaaaagga
    11581 gttagcgtct tagcagactt aagaaataaa gcatagtcca tatcggaatg tgcacccgca
    11641 tccagtgcat ccactaatcc aggttttgga cttaatgtta cttctgcgat caatgcttcc
    11701 tctaccaacg cagcgagttg ctgagtcact gctttttttc gttcgtcatt tatcatgaca
    11761 ctcactcctt cttcctacta ttacttgctg aaaccttttt tgatagctac ccaaatgata
    11821 tgaagtaacc agcttaaagc aaatgcacaa cttgccgtca ttagataagc tacaagaatc
    11881 atgactcctg aaggtaactg cagccaacta tctttaaaca ataataacag tacttggaaa
    11941 acaaatacat tggctaaata cgtacgataa gcataaacag acaaccattt gaacaatggc
    12001 aattttggcg aatcgtttac aatcattttg gaagcgccta tgaagacagc aaagatgatg
    12061 actaaactgt ataaactttg gatcgtattc aagtaaggcg cattcgcaaa gctcaagtct
    12121 cctggatgtg cgagcaatga acggacagaa aagaacgcga taactaaggc aacaggtaag
    12181 aaaagataac gtactctgtc caaatattga taaacttttt catgataaac aaatgcggct
    12241 accccataaa tcccgtaaat catgaaacta gcaacaaacc gatctaacaa gtaccagctg
    12301 tcatgataag gcccagtcaa tacttgtgtt tgatagaaca gataccatgc gacatataaa
    12361 atcgttgaac caatcaatac tggccataca tattttttat tagcaaatac tttgtaaccg
    12421 agccaaacaa aaaacggcat gaacaattgg atctgcagca tcataacggt ataccataaa
    12481 tgaggcgctc catcacctgt gacatatttc agtaaaaagc ttgcagtatc gtatacggag
    12541 tttgttgttg gactgctgga aatagccaaa gataagccgt cgtccataga agatacggaa
    12601 cgactagttc aaaaaacttt tctttcaaaa attcccaata agacaaatgc tggcttgttt
    12661 ttaccatatt ataaacaatt gcaaaaataa acattggtgc tgtgtattta acgatgacgt
    12721 aaaaggtacc aataaaagct gtatttgttg cgctgtggct gtttgccaaa gcaaagctca
    12781 atatcgtttg catcataaca gcctttacag ctgatacttt ctggaagtca cttacgttga
    12841 ttttgatttt tttgttttca aacatctctt tctctctctt tttctttatt accaaagttc
    12901 tttagttaat ttaggtcctt cgactgtttt caatagaatt tttttctctg gctctctcaa
    12961 atactctttt agtgaaaaac cgccttgccg agtcactact tgggtatcga attgctcaaa
    13021 aaaagcagga tgcgattgaa ttacctgaat tggcaattca atcggtgaat ttgcgtacag
    13081 tagcaaatca aaatcactgg tttcttttac tgccggaatc ccagtcgata attcaaatcc
    13141 taagctgccg ccgattcccc atttacatcc tgataatatg tttctagcag catgaaattg
    13201 ctgataaaca ggataatcca tgattttctc ttgtctatac gattcttgtt cgatgatgtc
    13261 ccacggatga atgacttgtt tgacggcatg ttttgagatt tctgttgccc atctctgatt
    13321 gcgggcaggt ccacgtaaac caatcggtag tatttctgcg tgatccgtcg acgcacgacg
    13381 aactgttccg aacatctttt cagttagaac gacccattca ggcaaggtga acccttctat
    13441 ttcatgttta tcaaaaagga caatatcatg tgcttgaatc attttttatg catcccattc
    13501 ttcgtccatt ttggcacgga ctttatttgt ttcgacacgt ccgaatgttt tggctgtttc
    13561 gtttgtgtat cgatttgtca gcatcgtgtc atttgtattg cggacatctt gaatggcttc
    13621 attgattgct tcgatgactt tgctgacatt ttgttctgaa gcagcttggt cggtgatatc
    13681 ttctaggaaa tgatagatcg ctcctagttt tgtataatta ttgatatcgt aagcgatagc
    13741 tggaactttt tcagcagctg cttcgatttc agcaaccgaa cgttttgtca cacgtgcagc
    13801 actttcttta gacatcgctt ggacggtaat ttctttattg tttaaagcaa tcaagcggct
    13861 tgattgtaat ccatgtgcta aaaatgcacc tgaaatagca tttcctgcaa tcaatccaat
    13921 aactggatat ccagcttgac ggaattttgc ataagcagca gcactattag ccaaagccac
    13981 atggataccg attaattctt cgttatagcc gtatgcttgg cttggtacat cgatcacaac
    14041 gatgattggc cgtttcactt ttttatcttt atcttcttcg taaacgtgat ccaaaatttg
    14101 tcccatgcgg aaaccttcaa ctaaaccaac ttcaccgttt cgtacacggt acatcgggtt
    14161 tttaggatca ggtacgattg atatcacgat ggtttcttct atgaatttac ttgatttacc
    14221 ataatacaca gtactaatag aagaagttgg atttttcatg ccagttagtg ccgcaaacca
    14281 atcgtaacca acacttgctg cagctggttc atttccttct gctacttctg gtaagtcaat
    14341 tgattttgct tgatggttag caattgcttc attgtaatcg tcaatggcca ttggttttga
    14401 aagatctaaa gaatcaagta atgataaata aaatgctgag ctttcactgc gatgcgtgtc
    14461 ttttcgttcg tccattgctg cgatgacagc agctttgatt gcttctacac tatcagtcac
    14521 taattcatca atgatttttg actctacccg ttgacgagat cctaatgtat tccaaataaa
    14581 gtctttgtca ctagaatcaa attctcgaac gcctgcttct tgttcaataa cttctggtcc
    14641 attcaaaccg actctcgctt tttttgttgc gattaaataa ctcattaatg ctgaagtaat
    14701 cgacataccg ccaaatgatc ctacacgtcc tggaatcaaa ccgatgactg gtacatattg
    14761 ttttagagca atgattacat ttccgatttc tgaaatagat aacagtccgt agtttgcttc
    14821 ttgcaaacga acaccacctg tatctagtac aatgactgga aagatctcat ttcctttttt
    14881 attttcttct aacacatgtt caagtgcagc aacgattttt gcaccggaaa cttcaccgat
    14941 accgccacct tggaattttc cttccatagc gattactact gcttgtttgc cattgatcgt
    15001 gccacggctg acaacgatcc cgtcatcact ttcagggacg attccttgtg ctggcaaact
    15061 tggcgcgatg atttgatcga atggatcaat caattcacgt ccattgtcaa gtagagcgtt
    15121 cactcgttct ctggcgttta attctacaaa actatttttc atctgaaagt tcctccattg
    15181 cttgcgtcaa tcgtaggtac acaactcctg gtgtagcacc gaagtcattg atcacgatat
    15241 ctgctgaaat tggataacga tcaaaaaatc tcgttaatac attgttccat acttcgccga
    15301 agccatcgct tcctgtacaa atttttacag tagattctgt tttttctgtt ggaaacatca
    15361 agatttccaa atctcctgaa ccgaccactc ctacatggat tttcttattg atcggctgtt
    15421 tcgttggata tttataagct aattgttcca tggatctctc ccctttttgt aaaacagagc
    15481 atacacgttc tgcttattcg tctttcttct tccatctggg taaatggcga caaaaggttt
    15541 ttctcgccaa ttttatgacc aagttctgaa tttcattggt ggttcataca ggccatctga
    15601 ccattcgatc aaatcatcga ttgtttgtgc agctaataat gaacgtttcg cttcactgcg
    15661 tttgatacct agatcttctg gtaatttcac gatgccggct tcacgcaagc tatctagtga
    15721 ttctttcgtg ctagtcattc caagaggtgt aacaccgcca attgctgcaa ttgctgcttg
    15781 acgttcttct tttgtttttg ctttgtacaa ataagcaatt ccttcttctg tcacgatatg
    15841 ggtagtatct tcgctataga tcatcactgg tgcattggct aagcctgcct gtttttgaac
    15901 aactaatgca tctaattctt cgacaaagac tggtttttta tttgaaccat atgtttcaac
    15961 catttgaaca actaattttt gtcctttacc taatggatca tcattgtcgc gaagtgattg
    16021 ccatgctggt gtagaatgac gacgaccgcc aggattatgt cccatgttag gcgctccacc
    16081 aaaaccagat aaacgtcctt ttgttacggt agaagaattt ccttcgtaat ctaattgcaa
    16141 ggttgaaccg ataaacatat cgattgcata ttgtccagct gcttgtgata atgttcggtt
    16201 actgcgcatc gtgccgtctt taccgacgaa aaagacatct gggcgagcag caatatattt
    16261 ttccataccg acttcaccac cgaaactatg gatactttca acccaacctg attcgattgc
    16321 tggaatcatc gttggatgtg ggttcaatac ccagttcttc gcgattttcc cttttaaccc
    16381 tagtgattca ccataagttg gcagtaataa ttcgatagct gctgtattga atccaatacc
    16441 gtgattcaaa gattgtacgc cgtgctctgc gtagattcct ttgattgcca tcattgccat
    16501 taggatttgc aattctgtga tgttttgagg atctcgtgtg aataaagctt ctaattgata
    16561 aggttcatct gcttttacga caacgtctac ccagtcacca gggatatcta cacgtggtaa
    16621 agaatcgaca acttcattga cttgaacgat cacgatgcca tctttgaaag ctgcagcttc
    16681 gacgattgtt ggtgtttctt ccgtattgaa acctgtatac aagttgccgt ctttatctgc
    16741 tttatctgca actactaaga caacgtttgg tatcaaatcg atgaataagc gtccataaag
    16801 ttccaaataa gtatggatat cacctaattt cattgtgccg tcttcaatca tttgggcgac
    16861 acgcaagctt tgtgcacctg cataagagaa atcgatcttg cttgcgattc ctttttcaaa
    16921 aatattcaaa tgttctggtc gtgagatact agacatgatc aaatgcagat cattcacttt
    16981 ttctgcatct actttttcaa gactttctga aagaaaactt gcttgttttt gattgtcgcc
    17041 ttctaataca actttgtcgc cgggagcaat cagtttttcc attacttcta cgattttttc
    17101 agtttctaca tacttgccat tgagcaagtt gctgattttt tccaatcgct gcgctttttt
    17161 ttcgcgttta gaagcccagt tgcggtttgg tttttccata attagtcatc tccatttttg
    17221 atatagaaac gtggcgtcat caataccatg ttggtctaga ttgatcgtac ggatcccttt
    17281 atctacacca aattttgtat gaatcagctt tgtcaaagta ttgccggggg gaaattcaat
    17341 cgtcagatcc attcctaatt cttttgccaa gtccatcatt tggttccact gcacagggaa
    17401 agctagattt tccaacaaat ctttacgaat ctcttctgcg tcctgagtgg ctcttccatc
    17461 cgtatttttt atatacaaac atctggcatc agatagatgg acagactgca tatacggctt
    17521 gaatttcttg accgtatcat tcatcaagag acagtgagat ggcacaggga ctttcagcag
    17581 cttcgctgtt tgcgcttggt gccgtttggc ttctgccaac gtaacggata aaccttcgat
    17641 actcccagat aacgtatgtt gcatcggaca attttcattt gacagataaa ctggatggtc
    17701 aggatcaaat gttttggcta ccatttcctc tgcttcgctt ctcgtcacac ctactactac
    17761 gcccattgca taaccgcttg gatatgcttc tttcatcaag actgcgcggt gatagactag
    17821 ttccgctgca ttctcaaatg tcaaagatcg acaagcaaca gctgcacaaa aagcaccgat
    17881 cgaatgtcct gctgcaatat caggctgaat accatttttt tccatttcat ctaaataaaa
    17941 tactgctttt gtcagtaatg ctagctggat aaaaaccgta tcttgatagt tttcttcttt
    18001 atcgactaac cgataccccg tcacattttc taaacgttcc atgtacattt cagggatatc
    18061 cgctaacatc cctaacttct gactgccttg tccaggaaat aagaaacctg tactcatgag
    18121 cctcgcctcc tcagcactta ctttttccac tgctatctta gtcaatttct gctataataa
    18181 aaaatgccaa aaaaagaaaa aaccataaca aaaaggtatg gatgggatgg ataattatga
    18241 aattgcttca tctgcagtat tttaaaaaag tattcgaaac aaaaaatgtt acccaagcag
    18301 ccaaagaatt atttatttct caacctgcct tgagccgtgc gatcaaacat ttggaaaacg
    18361 aattaggtgt ccctttgttt taccatagcg gcagaaacat tgagacgacc gtttatgcag
    18421 aagaatttta tccatatgca gtcaagacat tatacacatt agaagaagga atcaattatt
    18481 gtcttcggtc aatgagaaga tcgttacttc cgtcatattg tatttagagg tagcttctgt
    18541 ttctattccg aacttagtcc gaatttttat ggaaaagcat cctgatatcc aattaacgat
    18601 catgcaacat catttaccag atgatattac cgatcctgta ctgtacatca cttcggagat
    18661 gaagcctggc atgaccaata ttcctatcat gaaagaacct gtttatgtcg ccattcctaa
    18721 gaaacatcct cttgcgaaaa aagaacatct gcaattagaa gatatccagc gcacccccat
    18781 cttgatgctg tcaaagagca acgccttccg tcatacactg gatgcagcac ttgaagcgaa
    18841 aagtatcgaa ttgacgatca gttcgacgac agatgatcct gcgacattac gttctattgt
    18901 caagcaagga ttagctgtta gctttttccc aaaagtttct tggtcttacg ataaaaatga
    18961 tcctttcgtc ttgcgggaaa tcaaagattt gcccttgaca cgaacaattt atttatcttc
    19021 tagcttcaca gaagatcatc ctttgatcaa aatgattgca aaaacgatgc gggaatttta
    19081 tttaggaaaa taaaaaaaaa agagctggga caaaattctt gtcgcagctc ttttttcgaa
    19141 caaacggtgt tccagaagta atttcttaga aaataagccg aaatttc
//
LOCUS       DAIGOK010000051        18826 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.051, whole genome shotgun sequence.
ACCESSION   DAIGOK010000051 DAIGOK010000000
VERSION     DAIGOK010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.051"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(61..1761)
                     /locus_tag="NOA01_001410"
     CDS             complement(61..1761)
                     /locus_tag="NOA01_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632608.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            2268..3428
                     /locus_tag="NOA01_001411"
     CDS             2268..3428
                     /locus_tag="NOA01_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCI0632609.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            3444..3887
                     /locus_tag="NOA01_001412"
     CDS             3444..3887
                     /locus_tag="NOA01_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCI0632610.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(4370..4900)
                     /locus_tag="NOA01_001413"
     CDS             complement(4370..4900)
                     /locus_tag="NOA01_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HCI0632611.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            5005..5556
                     /locus_tag="NOA01_001414"
     CDS             5005..5556
                     /locus_tag="NOA01_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCI0632612.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(5709..6182)
                     /locus_tag="NOA01_001415"
     CDS             complement(5709..6182)
                     /locus_tag="NOA01_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCI0632613.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            6320..7792
                     /locus_tag="NOA01_001416"
     CDS             6320..7792
                     /locus_tag="NOA01_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCI0632614.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            7789..8499
                     /locus_tag="NOA01_001417"
     CDS             7789..8499
                     /locus_tag="NOA01_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HCI0632615.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            8851..9135
                     /locus_tag="NOA01_001418"
     CDS             8851..9135
                     /locus_tag="NOA01_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HCI0632616.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(9416..9835)
                     /locus_tag="NOA01_001419"
     CDS             complement(9416..9835)
                     /locus_tag="NOA01_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HCI0632617.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(9837..10421)
                     /locus_tag="NOA01_001420"
     CDS             complement(9837..10421)
                     /locus_tag="NOA01_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCI0632618.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(10468..11943)
                     /locus_tag="NOA01_001421"
     CDS             complement(10468..11943)
                     /locus_tag="NOA01_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HCI0632619.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            12124..12435
                     /locus_tag="NOA01_001422"
     CDS             12124..12435
                     /locus_tag="NOA01_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632620.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(12550..14886)
                     /locus_tag="NOA01_001423"
     CDS             complement(12550..14886)
                     /locus_tag="NOA01_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCI0632621.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            15028..15696
                     /locus_tag="NOA01_001424"
     CDS             15028..15696
                     /locus_tag="NOA01_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCI0632622.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            15699..16742
                     /locus_tag="NOA01_001425"
     CDS             15699..16742
                     /locus_tag="NOA01_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCI0632623.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(16979..17860)
                     /locus_tag="NOA01_001426"
     CDS             complement(16979..17860)
                     /locus_tag="NOA01_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCI0632624.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            18019..18747
                     /locus_tag="NOA01_001427"
     CDS             18019..18747
                     /locus_tag="NOA01_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCI0632625.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
BASE COUNT         5854 a         3362 c         3735 g         5875 t
ORIGIN      
        1 ctctccaaac gattataccg tttggagagt tttttgatcg gatgtattta tcccttttgc
       61 ctaagcacgt aaaagcttgt atctatgata aataaataaa catagcaagg taaaccagcc
      121 aatcaaagta agagaaagaa tgaaatagaa atagattggc gtttgataat gaagcgtgag
      181 ctcgttgatt cctttttgct gcttcacgat tggggtcccg atatctgtta cttcataatc
      241 agtcaatgtt tttccatttt gctgtagcac agtccgatcg tagacgataa cagggatatg
      301 aacagttttg tccccagtgt ttttccactt gattgtcaaa agaccgcctg gatgtgtttt
      361 tataaaagtt gtttcgtttt ctagtatttc ttcgttatac cggtcgtatt tattgtcttt
      421 tgtctcctta tagattggca gatagtcagg tgtggttttt tggaaaacga acaagctggt
      481 agacatatct ttgctgaaga atgttttcct tgcttcttcc ggcgtatcga atagataagt
      541 atgtcgactg acaaatgttt cattttccca cttttccaga tgctggtata agttctggat
      601 cgtctgtgct gaaaaaataa gaatgccaaa tatacttaaa agacggaacc acttttgttc
      661 agaccactgg ttcagtgaaa gggcaaggta aagtagaagc aatagcgtaa atggcacgaa
      721 aaatcgaaat ggaaactgga tcaaactaat aaaaggcaca tttttttcga tcaatgtcgt
      781 ccatggaatc aagctagtag aaagaaaaaa gaaaacaaga cttgtgtaca acacgatttt
      841 ttggaataag gtgttctttt gttgaaagaa aataaaaagc aaaaaacctg ataatatcaa
      901 tataggaaag gccttagggt aatggagcca ataactgcca tccagatcta tcgccttttg
      961 actcatttct ctgttaacaa agggaggaag aatcttattc ccactgtaaa caatagctaa
     1021 tcctgcccaa atatttgctg tcagaacaaa ataaagcaag accgacttaa tcagttgaag
     1081 cagtgctttt cctttttcag tctggttaaa aaagtaataa agataaaaag ggatatacat
     1141 caaagctaac atgatcgaag acagtaaatg gatctgtgtc ataagtccca ctgagacagc
     1201 gactttgata acagggaact ttttatgaag gacgaaatca gcaataggga tcatacataa
     1261 aggataaaaa caagtccccc agctagtgaa tccttggttg atagaccagt attgaataga
     1321 atacgtactc atgaataaaa cggctactgg tacacttacc caatttcgaa cattgacata
     1381 tctcagtaaa atcagcatag aaaatcctga aataagataa agaatgaaat ttgaaagaat
     1441 ctgataatga aaccagctac ctgcaagcaa cacgatcaat ccttgtaaat aagcgaaaaa
     1501 tgggccgtaa acggcattca cgatcctgcc actttgctgg aatccataaa gggaaggaaa
     1561 ataatggaaa tccccatatt taatttgttg cgcggcatca tagaaccgat tgtaatggaa
     1621 tggggcatca gcacccaaaa ttagtttatg tgtgaataaa tgaggcatga gaaatgccaa
     1681 agcaatgaaa aaaattgcta aaaatgggcc atattttttc aattgtcgaa ctcctttgca
     1741 aactttagat aaattcatca tatatcctat gcgagaggta ggcaagaaat tagaaaattc
     1801 atgaaaatat ttatgaattt tacaagaaaa acataagtga aagttctaag gagagaggat
     1861 gcgacataag ttttgttgca tctccttgtt ttgaaacaga atttttctta atactcgaaa
     1921 caaagtagct ttttcatgac ctctcggtaa acggtgttca atcagctgct tctcggtaat
     1981 aagctcacta gcctagaaaa tatgagaagc tattttctga ctagcgctct tattactcaa
     2041 agcataacgc agttttcaca acctcttcct taaacggtgt tcaaaagctt gcatctgcgg
     2101 taataagccc aacccaacaa aaaatatgag tagctatttt tttgggctgt acttattact
     2161 tgatgcagga cagctttttc acaacctcat cggctttcat tttctatttt tttcctgaac
     2221 ccatataata aaagaaagaa caaaaaaact tgcaagggag gacacatgtg aagcagagta
     2281 agcaaaaaca gtctcgtcga cttttgtttc gtttgttttt attaatatgt atcgtcgtag
     2341 ggttactgat cgctttatat ccattttatg tggattcttt aaatagtttt attgatcaaa
     2401 aacgtatcga acaagtacaa aaacggaccg cagctgaaat tgaagtgcag cgaaagaaaa
     2461 tggaggagaa aaaccaacga ttaactgacc aaggattcaa tcctggtact gatcctttca
     2521 atgaaaagaa cagaaatgaa agtacaacat ccagccaatt agaggaatgg ctgatcggaa
     2581 gtatcaatat acctaagatc caattaaata tttcacttta tgatcggctc aatggtagga
     2641 tccttgaaaa tggagcaggc gtcttgcaag gaacctcttt cccgcttggt ggaaattcga
     2701 cgcattcagt gatctctgct cacagcggtt tacctaatcg acggttattt accgaattag
     2761 atcgtttaga aaacggagac acgtttattt tgactgtttt gggggagaaa ttggcttatc
     2821 aagttaaaaa tatacaagtg gtactgccag atgacacgtc tgttctaacg atttcagaag
     2881 gagaagactt agttaccttg cttacatgta cgccgtatat gatcaacacg catcgtttgc
     2941 tagtgacagg acatcggatt ccttataacg aatcggtaaa aaaagaagaa ggaaaaggaa
     3001 atcaggaaag aaccatgcgg caatggttta ttcttgcagg aacgataata gctgtagtca
     3061 tactgttagt gtttatcgga cgactaatct atcagtatag attaagtaaa aaagtgcttg
     3121 atttttcatt tattattagt gattcgtcag ggaaaccggt aaatggtagt tcattcattt
     3181 tgaaacataa gaaaaaaaca cttactagaa atggtgtgcc gttttcagta caaagcgacc
     3241 atcatgggaa agtcaagctg gatcaattgc ctggtggaac ttatcgaatc gtttcagctg
     3301 atgatcctaa agttgctgtt tcttttggta ttagaaaagt aaaacaagag aagatatact
     3361 tttttgaggg gcgaaaactt gtaaaagagc ttcaaaaaaa tggtttttat tttaaaatca
     3421 atgaataagt aaggagagaa aaaatgatcc gcacaacaac aaaattgtct gccaaagaac
     3481 tggaatctgt tctatcgatt tggctgcatg ctaacttgga ttctcatgaa ttcattccat
     3541 caacttattg ggagaatcaa gtagaatctg ttaggaaagc tttaccagat gcagtgattt
     3601 acttagcaga aatagatcaa gaaattattg gttttgctgg ggtgattgat cagtttatcg
     3661 caggaatctt tgtgaaaagg gaataccggc aacaaagaat cggtacgcaa ctaatgaaaa
     3721 aaataaaaga aaccaataaa acactgactt tgacagtcta tcaaaaaaat caatcagcta
     3781 ttcatttcta tctttcacaa ggttttacaa ttcaaacaca gcaaaaagat gaagcagtaa
     3841 atgaggagga acttgtgatg aaatggaatc aaaacgctga tttttgatga acagaataac
     3901 gaaataatgc tcccagccta attttttgct gggagcatta tttcattttg aagtcactaa
     3961 tttaactgtg tgaatatgca gttgttacaa tctttgataa acggtgttca atcagctgac
     4021 cctcggtatt agctcaaaaa acggcaaaat atgaggaact atttttccat ttgtttctct
     4081 aatactcagg tcaaaacgct gatttcacaa cctcttgata aacggtgttc aaacggctgc
     4141 ttctcggtaa taagcccact agcctagaaa atatgaaaag ctattttcta actagcgttc
     4201 ttattactca aagcataacg ctgatttcac aacctcttga taaacggtgt tcaatcagct
     4261 gaccctcggt attagctcaa aaaacggcaa aacatgagga actatttttc cgtttctttc
     4321 tctaatactc aggtcagaac gctgatttca caacctctct ttcacaacct tattttactc
     4381 gtttataatt ttcatcgaag tattttccgc tacggatatc atctggcatt ccatcgtatg
     4441 gttcttcgct aataacatct tcattgtcta tacctgcatc acccatataa gtgatcgtag
     4501 caaattcagg aacaagtaat tttggataag tttcatattt ttcacgagct gcttccattt
     4561 ctgcgatatg ttcgttttgg aaagtaacag tgatttctgg atgttcttca acccatttag
     4621 ggaaaaagat cattccgtga agtttctttt catttggcat aaagtccaat gccacgtctg
     4681 tccctgttgg ctctgtccaa gtgattttga atacgccgtc cgtcaattta acgatatcag
     4741 cttcttggtc ttttacccaa cggcctttga ccattccgcc atggatacgg taatcaacag
     4801 tatggtcatt tttggcatac cattcatatt cccaaccatt gtcatacgta tagataaagt
     4861 gagttccaat aaaatcttcc aattctttaa attctttcat gtttaagcat ctccttataa
     4921 attaaaacat gtaataaact acatgttttc gattactcat ttatgataga atggatttaa
     4981 agatttgtca aggagggaaa actgatggca agaaaaaata cacttcgata tattttgtta
     5041 gggttattaa gtaagaagaa gatgacagga tatgaattga atcaatcgtt caaaaatgaa
     5101 ataggagaat tttggcaagc aaaacatagt cagatttatc ctgaacttgc aaaaatggaa
     5161 gagcaaggaa tcattcagca tcaagtagag atcacaggtg aaaaattgga aaagaaagtt
     5221 tatgaattaa cagaagaagg gaaggaacaa ttggcagagt ggattcatac acccaccaat
     5281 gaactaccgg ttaatcgaga cgaatttgtt ctgaaattgt atttcgtcaa agacgtgaat
     5341 gatccggcat tagtagaaat tattcagcag cagcgtgacc ttcatgaaga aaaacgactt
     5401 catctattag agcgaaaaaa aacgatcttt cctactgaaa aagaaaaaca tgaaaattac
     5461 ggacactacc tgattttagc gcatgcgatc aatcgggaaa cagaatatgc aaattggcta
     5521 acagatgtat tagaagaaag taaccgaaaa aaataaaaaa atatccgaac agtgatggag
     5581 aattcctttc tatatgaaag aaacgcagat caccgttcgg atattcttat gttcaaacag
     5641 gccttttttc aagtatttat gtagcatcta cttattaaaa agcttggtac ttaggagatt
     5701 agtggcgttt agagttcttt taacaactgt tcgtagtcag ctttttcatc gacaggaagg
     5761 agcttgtaag ttaattcgtc tgtctcgttg tcatgaatga tgtacatctt agcattctct
     5821 tctttgaagt taccgatttg taaaatcgta tagcgttcgt cgatcaattc gatatctttg
     5881 attgcttttg aaaaaacttt acggacatgg tgcattttct tttggcggat ttctgccttt
     5941 tctgcagctt ttgacaaagt aaatccgtca tctaaatatt ttttgatcag ttctactttt
     6001 atgactgtag gtaaacgata ttttctagga caattcgcat cagcttcgat cgaacgaatg
     6061 aatccctttt gttcccaata ccttaattga cgtggagaca cacctacgat atcacttaat
     6121 tcactgatgc caacaaccaa ttgatcattt tccaacaaac gtttaaggga agccccaacc
     6181 atttttgtca ctccttttgt cctcttgctt cccttaatta tatccgtatt aacttatgtg
     6241 tcaagtaggt tgacaaatag cgaatttagg actaaagtat tctagtagtt tcagaaaaaa
     6301 ttcagaaaga aggagaaata tgagtaacaa ccaacccgta gatatttacg gaaaaccata
     6361 taatcggaca cttttagttg ttgtcttatt aattggtaca ttttgtaccg ttttgaatca
     6421 gaccttattg acgacagctt ttcctacatt gatgaaagct ttcgatattt cagcgtctga
     6481 tgtgcaatgg ttaacgactg gtttcttgct agtcaacggg atcatgatcc caataacagc
     6541 atggctgatc aataaattca gttcaagaaa tttatatctt tctgccatga cgatcttttt
     6601 gatcggaacg attacttgct ttacagcacc taattttagt acattactaa tcggtcgttt
     6661 gatccaagct tgcggtgtgg gagtatcgat gccattattg caaaatatca tgctatctat
     6721 tttcccgcca gaaaaaagag gatcagcaat ggggatgtca gggatcgtta ttggacttgc
     6781 tcctgcttta ggccctacat tatctggtta tatcattgat aactaccatt ggcgtgactt
     6841 gtttggtatg gtactgccaa tcgttgttct tgtcttggtt ttagcattct tcctaatgaa
     6901 aagtgttatt ccattgtcaa atcctagcat tgatatttta tccgctattt tatcaacgat
     6961 tggattcggt agtttacttt acggattttc tagtgtagga gatgacggct ggggaagctt
     7021 gaaagtaatt ggtttcttag ttgttggtgt gattttcatc gccttgttct catggcgtca
     7081 attacatttg gaacatccat tcttggaact tcgtgtattc aaatcatcta cattcacgat
     7141 tgcagctgtt ttagctggtg taacaaatat ggcgatggta ggggcagaaa tggtcgtacc
     7201 attgtatatt caaaatattc gtggagaatc agcattccat tctggcttga tgttgttgcc
     7261 gggtgcattg atcatggggg ctatgatgcc tgtgacgggt gcgattttcg ataaatacgg
     7321 cgcgaaacgt ttagccatca cagggatgtt cattttgaca gcggcaactg caccatttgc
     7381 tttcttgaca aaaacgactc cagttttata tatcgtgatc ctttatgcga tccgtatgtt
     7441 tgggatctcc atggtcatga tgccggtaac gacttctggt atgaatgcat tgccaaataa
     7501 cttgatcagt catgggacag cggtaaataa tacattccgt caaatcgcaa gctcgattgg
     7561 tacagctatt ttgatcagtg tgttgacgaa tgtgacaaaa gcaaatatcc caagcaaatc
     7621 tgtattggat aatacgccat tagcttataa agatcaggca atcaatgcca cactgtcagg
     7681 ttaccatgcc gcgtttatcg tagcgatcat ctttgggatt cttggcttta ttattgcctt
     7741 tttattgaag aaaaaagaac ctgttctaat gaaagcgggg gataatgcat gattattgga
     7801 gttttgattg tcagcttgct tgcttttgct ttaacgaacg tgttcgctaa aaaaacatgg
     7861 caaacctttt tatctttgat tttcggacta atcttcgtcg cttcactaag tttgatcgtc
     7921 gctaatttgt ccaatcactt tggaatggaa aaagtaacgg aaacaaaaac agaaaaaatt
     7981 gtttccagtg ctgattccca aggagcagat atgcttttgt ataaagcttt agggaatgga
     8041 aaagaaaaag tgtatcttta ccgaacaaat gaaaaacaag aaaaaccaaa agcaactggt
     8101 acggataacg aaacaaataa agtagaaaag acagacggcg atgctgaaaa agtgacgaaa
     8161 actacttatt gggaatacaa aaacgacatg tataaattct ggttcaatat tgctgataac
     8221 aatcacgaat atgacagccg agtgaataca ttcaaaatcc cagagacatg ggtagaatta
     8281 agtacggacc aagcagccaa gttagctgaa ttagtgaaga aacagcaaag tacgatggaa
     8341 agtgaagcaa aagcttatgt tcaagacggt atggtaaaag caatgactga aaatccaaaa
     8401 atgagcaaag cagagcaaga acaacgtacg aaagccttag ctgctgaatt ccaacaacaa
     8461 gcattcgcaa aattagtgaa agaagcaaaa ggcgaataat attttgaaca ccatgtagga
     8521 ggttgtgaaa aagctgctct gcatcaagca gtaagaacaa tcagataaaa atagcactcc
     8581 atatttttca tctgattggg cttagtgccg tagatgcaag cttttgaaca ccgtttaagg
     8641 aagaggttgt gagcctgccg tttaactctg agtcacaagg agcaatttca aaaaacagct
     8701 tctcatgttt gttgactatt gtgacttgtg accgaggagt tggcaggaga acaccgttta
     8761 ttcgaaaaaa ggaactgcga caagactttt gtcacagttc cttctttttt ttgctatgct
     8821 atactagatg taaaaaatag gagaaataaa atgagggaaa tttattcttt tgaatcaaaa
     8881 atctatgctt ccgaagtcgg caagggcggg gcatatatta tttttccgta tgatataaga
     8941 aaagaattcg gtgctggaag agttaaagtc caagcaactt ttgacaatgt gccgtatgaa
     9001 ggcagtatcg tgaatatggg cgtgaaaaat ccagatggtt cagtctgcta catccttgga
     9061 ctacgaaaag atattcgtaa aaacatcgga aaagaaatcg gtgatattgt agcagttaca
     9121 gtgaagcaaa aataaaagag gttgtgacaa aagacgtgtc acaacctctt gataaacggt
     9181 gttcaaaagc cagtttctcg gtagtaagtc aaatttatgc aaaaatgtgg aaacccatgt
     9241 ttacaaaatt tttcttacta ctcgaaacga agcggcttta tcacaacctc ttgataaacg
     9301 gcgttcaatc agctgtccct cggtattagc tcaaaaacgg caaaatatga gaagctattt
     9361 ttccattttt ttctctcata ctcaggtcat aacgctgatt tcacaacctc ttctttcata
     9421 atggtttttc accatcttta taataaactg gttctttgat atggaaatct attttttcca
     9481 accaatctaa aatcttgtaa cctaactctt ctgacatcgg aaaagaggcg ttgatttgtg
     9541 cattggagat atggtcaaca tggataccgg aagtgatcgt acaacttccc ggaacggcag
     9601 ttttgatgat cacagctatt ttttcggcta agacatcgtc tttatggaac cgaccatcgt
     9661 ggctagggaa acgtacagtt tgcatctctg tatcacgaga aaatgtagtg actgttccga
     9721 tatgtggtgt atctccgcct gttaaagtga tcaataagtc tttaccgatt actttaacag
     9781 cagcttggat cgtgaaacct agtttggtta cttcaactat cgtttctttt tccatattaa
     9841 tcaccctgcc aacgttttga atgctcattt acaatttcaa attgatccaa aagtttcatt
     9901 gtatggtgat taacaagatc ttggatcgtt tgaggctgat tataaaaagc aggaattggt
     9961 ggtatgattt gcacacctag tcgtgctaat ttagtcaagt tttccaagtg aatggcattt
    10021 aatggcgttt cacgaggaac aatgactaac tttcgttgct ccttcattgt tacatcagca
    10081 gcacgtgcaa tcaggttgtc gcctaaacca acagagatac ctgcgactgt tttcatactt
    10141 gccggtacga tgatcattcc atcggtcaaa aatgatccgc tggcgacagc agcacctaaa
    10201 tctttgtttg aataatggta atcaaacagg ctttcgaatt cagttagtga taagtcggat
    10261 tccagcttta agttctgttt tgcccatgga ctgatgattc cgtgtgtttc tatcatcgga
    10321 tattctttta gtttcttcac taggttgatg gcataaatgg tgcctgaagc gccacttaca
    10381 ccaatcacga tttttttctt ccgttgtgcc aattgttcca ttttccgcac tcctttttat
    10441 gatgtcaaaa cagttgcaga tcattcatta tttcaaatat ttttcccaat cttctacttc
    10501 ttggaatttt gcacgtacaa aatgttcttt catatcgaaa ggaactgtac cgtcaaagat
    10561 agtttttgca cttatcccac ggaaacgaat agagccagga ttatattctg gtaattctga
    10621 tgggtccaat ggatggttcc tcattcctgg gatcacgatg atatctttat ctccttggaa
    10681 acgagtgttg aaagtccaaa ctacatcgtt catgtcgaaa atatcaacat cttcatcgac
    10741 taaaatgact gtcttcaatt ctttgaaggc tgataaagca agaatagctg cttgacgctg
    10801 aataccttca tccgcttcgt tttctttacg gatttgcatg atggtcatca atttaccacc
    10861 acctgcagga ggattgtaga catttactac ttttcctgga atagctttat cgaccaaggc
    10921 aagaatactt gcttctgtag gaataccggc catagatacg tgttcttcac tgggaccaat
    10981 cgttgtttgc atgattggat tgtcttttcg gtgagtaact gctttgactt tcactacttg
    11041 tacagctgga ttagcatccc cagtatagcc aggaaattct ggcattgctt ttcctgagtt
    11101 cgtgttgata tcttcttgca ttgtttcatt tggcatgatt tctgcttcaa tgatgaattc
    11161 agcacgagca atacccactt catcgactgc tataccatca acgatttgaa taggttcgtt
    11221 acgcaaagct cctgcacacc atagttcatt gtagccaagt ggagtggtag gtggttcgaa
    11281 agtcgtgcca atggcaattg ctggatctaa accaatattg atagtgatag gcattggttt
    11341 attcaaggct tcgaattgtt tttggaagtg accaatgtgg cgtccgccag gcatgatgta
    11401 cataccgatc gtatctttat cttctagaac catacgatgg atcgttacat cgctcattgt
    11461 tttttctgga tctgatccta agactacacc cattgtgatg tatggaccag catcttcagg
    11521 agtatttgta ggtgcaggca aaatgttgcg aatatcgaag tctgcatcgg tagctagatg
    11581 aacgacttct tgtgctggag cgtctgcttt gtctactttc acaggtttga ttggattttc
    11641 tacagcgtct ttcaagaaac gaccaagtgt tcgataatca tggtgcaaaa tttcacctac
    11701 acgtttacga ctagccattg ttccgatgtt cacacgtgtg ccggggaaac ctttgatatt
    11761 gttgaaagtc atagctggtc cttcttgagt cggacgtttt actgtaccgc cagcaccgat
    11821 ataacggtaa acaccagata attctgcttc aggatcgatt tcaatatctg tttcgtgata
    11881 ttgacctgga agtgttttta gttcttcgat gacttttctt aaatcatacg gttgatctgt
    11941 catgagagat tcctccttag ttgtctttac ctatctatag taaaaggtga aaaagaggaa
    12001 aacaattcaa cttgttcggt aaagtattct acaatggaat ggatgcgttt ttttgataat
    12061 agaataaact atgatatact tatcactaaa agaaagcgca tacttagtgt tggaggaagg
    12121 aaagtgaaaa aaatggagga gtcatttcct aaagcagtaa aagtggaaaa tatagcgaat
    12181 attttgaaag ttacatttga aaacggagaa gtaaaatacg tgaaatctca ttggacagaa
    12241 gagataaccg atgccttgca atttggaaaa aaaggaagag gaaaaagaaa aaatcttctc
    12301 gcactttctc gtaatatgtg gattgggact gaagtaacga ttgaagcaga tggaacagta
    12361 ttcataaatg gaaaagacag gtatacgcca gaagagttgt ggtacaaagg aaagaaaagt
    12421 ataccagaat tataaaaaat aattaaaaaa aacagcgcga aacaaggaac atattacttc
    12481 ctcgtttctt gctgttttct actatttcag cgctcttatg actcaaatct aaatgtggtt
    12541 gtcacaacct tattctgcac gcaaagcgat agcggcgtct tttttcgctg ccattcgtgc
    12601 aggaagatga ccgccaagca tcgtcaatat agtagagacg atcactagaa taatcgcatg
    12661 gacaggattt agttgagcaa cattttttag atccgtcatg ttgtataaga tgctattgat
    12721 agggaacgta gctagccatg caataaacac acctaatatc ccagaagaaa tacctaaaat
    12781 acacgtttct gcgtcaaata cgcgagtgat gtctttttta cgtgcaccta aagcttttag
    12841 tacaccgatt tcttttgtcc gttcaatcac agatgtataa gtaatgatac tgatcatgat
    12901 catacttgtt actaaagaga tccctgcaaa agcaataagg acataagtga tagcatccat
    12961 caatccgcca gtcagttcag tcatcgttcc agctaaatca gtataaatga ttttatcttc
    13021 atctgatttg cctttattgt aatcatctag ataatccaag atcttttctt tgtcttcaaa
    13081 attgttcgga taaatcatga tacttgaagg caagctgtct ccacctaaat aactaagcaa
    13141 tgtttgtttg gcattttcgt caactttttc agtagttaag acatttacac cactgtctct
    13201 ttgagctttg acgatttctg attctttgtt ttcgttgacg atttgagtcg ttaattgatc
    13261 gctataagcg attcctggag ataagagatt cattgtagaa gaagatttca cgcgtaggat
    13321 ccccgagatc ttcaattcat cagaagcatt ctgatacatc gcatcataat ctgtattagg
    13381 gataaaattc ccagttggaa gttttgtata gtaagtattg ttgtttacta atttgaatgt
    13441 tgtaccaacg atatcatcaa aatcaagttt ttcgtcttct ttcacatcaa atccaagatt
    13501 cttcaatgcg ttgatattag tattattatt gccgtctacg atcagcacga catctgttgc
    13561 tgaggctgga taacttccgg caagtaaaga ataattgtct tttaaaaagt tttctttgct
    13621 agtgtcaagc tgtgtaggga aacttgacac tcccacacct gtcattgctg acatcgtact
    13681 agataaagaa agagattccg tatcaggatt ttgattcgag aagctgacag gctgtacttt
    13741 gccatttaca tcacgtaaaa gattgattcc tgtagtacgg gtaaatccga tattattact
    13801 taagttagga tcgatgtctg ttacgtaatc gacgtatgtt tgatcaatct tattcgtgtg
    13861 ctgtgcttta tcttcttcgc tgacttttgc agtaatcgtt ttgctgtctg gataatccgc
    13921 tttgcttgca cccaatccgg catcgtcacg agtctggtta gtcgttacct tagaaatagt
    13981 aatcggaaat ttcgccatcg tttcagcttg cgtgttatcg atttgttttt ggaatccaga
    14041 agaaagggac agaacgatcg cgattccgat gatcccaata ctggaagcaa aggaggtcaa
    14101 gaaagtacgt cctttttttg ttcggatatt gttgaaggaa agctttaacg ctgtccaaaa
    14161 actcatcttt gttctgcgta agttaaattg atcatctttt ggtcgttcga tgtgaggatg
    14221 ggaatctgtt aagatcttac catcagaaaa ttcaatgatt cgatctgcgt attgatgggc
    14281 cagttcagga ttatgtgtga ccatgatcac taatttttct ttcgataact cttggatcaa
    14341 ttccatgatc tggatacttg tttctgtatc taacgctccg gttggttcat cgcacaaaag
    14401 gatatctggg tcattggcta gtgctcgagc gatggccact cgctgcattt gtccaccaga
    14461 taactgattt ggctttttat gcatatgatt tgtcaagccg actcggtgca atgcatcttc
    14521 tgcccgtttt cgtttttcgt ctttagaaac accacttaat gtcatcccaa gttcaacgtt
    14581 ttcaataatg cctagatgac caatcaagtt gtaactttga aaaatgaaac cgatagagtt
    14641 gtttcggtag gcatcccagt cactgtcctt aaagtcttta gtagactttc cgttgattac
    14701 catatcaccg gaatcataat tatctagtcc accaatcaca ttcagcatag tcgttttacc
    14761 agaaccacta ggacctaaaa tcgctacgaa ttctttttgt ctgaaagcga cagacacacc
    14821 atccaatgct ttggtggtgg tttcgccgac tttataatat ttttttatat cttttaattg
    14881 aagcataatc acccttctcc cgtattgaaa tattcggtat actaataagt atatcagaac
    14941 aatgatcgta gtatacgaaa taaatgttaa ctgaagatga actacaagaa aaaatctaga
    15001 aatttttcaa aaaacagagg gagatcaatg aatcgtattt tagtagtaga agatgatccg
    15061 caattacagt tgctttaccg ttctgtgttg gaacgagcgg gcttttcagt catcaccgca
    15121 ttgaatggag aggatgcgct gaaaaaacta gaagcgacac gtatagaatt aatcattaca
    15181 gatatcatga tgccgaaaat ggatggctat gacttgctag actcattacg tagcaccaga
    15241 tttgacacac ctgttctaat gattacggca aaggccgatt ttgaggataa gaaacgcggg
    15301 ttcaaattag gagcagatga ttatatgaca aaacccatcg atgtcaatga gatgatccta
    15361 cgtgtggaag cattgcttcg acgttcaaag atcaattaca gcagtagctt gataatcgga
    15421 caaacaaaat tagagctaga tgcttataca gtgttacaag atcagcattc gttgattctg
    15481 cctcaaaaag aatttctgct tctgtataaa ttactgtctt atcccaacaa gatcttcact
    15541 cgccaacagt tgatggatga tatttgggga ttagatacga atacagaaga acgaacgatc
    15601 gatgtacata tcaaacgttt aaggacccgt tttgaaaaga cggaagattt tcaaatcatc
    15661 acagtacggg gactaggcta taaggcggtt atttaaagat gaaaaaaaag ctattttcac
    15721 aattatggat ctattttgct agtattgtct tcatttcgat cttagtgaca ctgctgtgtt
    15781 ttctcggttt tattttctta ttgtctcgac attctatctc gcctgctgaa tcggctggaa
    15841 aaccaacgtt atacccgtta ttcgtctttg cggggttcag tatggtcgtg ggtacaggaa
    15901 tatcgatttt tgttgggcgg cgtatcctgc atccaatcag tgcgctaggg acaaatatga
    15961 gtctggtagc aacgggagat ttttcgattc gaatggatga acagcagaaa gtagcagaag
    16021 tacagcaact gtataaagat ttcaacgtta tggtccaaga actcaatagt atcgagacac
    16081 tgaggaatga ctttgtgtcc agtgtatccc atgaattcaa aacaccacta gcaacaatcc
    16141 aaggatatgt tcaattgtta caagccccaa atctctctga cgaagaacgc caaatatttt
    16201 tatatcggat tatcgaaagt atcacgcagc tttcacaatt aacagagaac atcttaaaat
    16261 tgaacaaact ggaaaaccag cgtatccaac tggaaaagaa agagtatcga ttagatgaac
    16321 aaatcagaga agtaatcgtt ttttttcagc ctaaatggga gaaagaacag ctagaattgg
    16381 atatcgaact tgctgcagta aattacacag gcaatgaaga gtttttgtac caagtgtggc
    16441 tgaatatcat ggataacgcc ataaagtaca atcaaatcaa tggacagatt catatcaaac
    16501 tatttgagac agccacagag attgtactgg aagtgaccga ttcaggtgta ggaatgaatg
    16561 aagagaccag agatcgaatg tttgaaaaat tttatcaagg agatacaagt cgtcaaattt
    16621 ctgggaatgg gctaggactt tcactggtta aaaaaattct agaactgcat gatggacgta
    16681 ttgattatag cagtatcgaa ggagtaggaa cgacagcaat gatacggtta agtaaacaat
    16741 aggaaaaaga ataaaacccc taaaaattct attgaaggtt tttaggggtt tttagtcgtt
    16801 tttttgtgat tagggaaatg ttcttacggt tcttttggca taaaaatcgc tgctttctta
    16861 ttgaactacg agaaatgtta gaggtgtcat tgaaacaagg acatgatgca agaaagagag
    16921 cgaaagtcat gcgtcgctct ctttttttgg gtggatagat ggacagaaaa tgtagggctt
    16981 agttttcttc ttgatttaaa tactcccaaa atgtatctga agccaaagaa tgctcaccga
    17041 ctttacgagt aaatgcaaga taactctctg ttgttggaga aatagggaga atcacagtat
    17101 tttcttttaa tagattcttg actgtttttt ccataacaat agcaatcccc atgtcatttg
    17161 caatcatatt gatgatcaag tcgattcttg ctcctttata agagattttt ggttcaaatc
    17221 cagcttcgcg acacagatct agtacaggtt gatacaagtt cgtagtgggg ccaaggagca
    17281 aaaagttctc ctttttcagt tcgcttagat ccaatttctt tttactagct aaaggatgat
    17341 ttttaggtag aactgctaca aaatcgtctg tttcagtagg caaccactca atcgaacttg
    17401 gtggggcctg gaaacttcta gcaaagtgga tcgtgtcact atgttcattg gcgtcattgt
    17461 aagttaattc gacactttcc acttctttca actggactgt aaattcagga tggcgtttca
    17521 aaaactcggt gattttggaa aaacctttat aattgatcat tgtcggtatc gttaagatat
    17581 ttaatgaaag attgttgtct ttcgtatagg tttcaagcac ttcttgcata gcatggtatt
    17641 gttctacgac attttttaca tatgggagca ataagcttcc agcttcggtc aattcgattt
    17701 ttctatgggc gcgttcgaat aatttgacgt ctaattctgt ttctaacgaa agaatctgtt
    17761 tagaaatatt tccttgggtg gtgaattgtc gatcagcagt tttggtgaaa ttcattgttt
    17821 cggctaaatc aagaaaaact tcgaatttat ggatatccat catattcctc ctacttattc
    17881 caataaagaa tactttaaca caaaaaatga attggtgtaa tcttctctgt ctgttacaat
    17941 ctttcttgca gcgaaagcaa taattacaga aatttttctg atttttttcg cttcgtagaa
    18001 aaagagagga gttttttgat gccaactgga gttttgatca atgtaggttc cgttttgttt
    18061 ggcggactaa ttggcggatt agtaggaaat aaattaagtg aacattttaa agcacagctg
    18121 acgatggttt ttggtgtgtg ttcgatgggg atgggaatct attccattgc gccgatgaaa
    18181 aatatgccgg ctgttatttt tgctttagtg ataggaacag cgattggttt gattgttcat
    18241 ttaggtaatg ggataaataa gggggctgcg ctgatgcaag tacctatttc taaaatcttc
    18301 cctagtgaaa agcttggtat gacccacgat gaatttatca gcacattagt gacggtcatt
    18361 gttctatttt gtgcaagtgg tacggggatc tatggaagtt tagattctgg gatgacagga
    18421 gacagtacga ttttgatttc taaatctgtc cttgattttt tcacagcagc tatttttgcg
    18481 tgtaatttag ggtatgtcgt ttctgtgata gcaattcccc aattcattat tttttatatt
    18541 ttgttcttat tggcaaaatt tatttatcca ttgactacac cagacatgat cttagatttt
    18601 aaagcttgcg gcggattctt gatggtcgct accggtttta gaatgatcaa cgtgaaaatg
    18661 ttcccagtag ctgacatgat acctgcaatg atcgttatca tgccactgag ctggatgtgg
    18721 acaaattgga ttatgccact gttataataa aaatcaaaga gaagccattt tttgctgact
    18781 aaaaaggatc aaatctttta gtcagttttt ttgtatttga caaact
//
LOCUS       DAIGOK010000052        18573 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.052, whole genome shotgun sequence.
ACCESSION   DAIGOK010000052 DAIGOK010000000
VERSION     DAIGOK010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.052"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            29..781
                     /locus_tag="NOA01_001428"
     CDS             29..781
                     /locus_tag="NOA01_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCI0632626.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            778..1692
                     /gene="pfkB"
                     /locus_tag="NOA01_001429"
     CDS             778..1692
                     /gene="pfkB"
                     /locus_tag="NOA01_001429"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCI0632627.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            1710..3641
                     /locus_tag="NOA01_001430"
     CDS             1710..3641
                     /locus_tag="NOA01_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCI0632628.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(3752..5311)
                     /locus_tag="NOA01_001431"
     CDS             complement(3752..5311)
                     /locus_tag="NOA01_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HCI0632629.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            5466..7700
                     /gene="pcrA"
                     /locus_tag="NOA01_001432"
     CDS             5466..7700
                     /gene="pcrA"
                     /locus_tag="NOA01_001432"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HCI0632630.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            7717..9753
                     /gene="ligA"
                     /locus_tag="NOA01_001433"
     CDS             7717..9753
                     /gene="ligA"
                     /locus_tag="NOA01_001433"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCI0632631.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            9859..10164
                     /gene="gatC"
                     /locus_tag="NOA01_001434"
     CDS             9859..10164
                     /gene="gatC"
                     /locus_tag="NOA01_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HCI0632632.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            10164..11636
                     /gene="gatA"
                     /locus_tag="NOA01_001435"
     CDS             10164..11636
                     /gene="gatA"
                     /locus_tag="NOA01_001435"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HCI0632633.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            11633..13063
                     /gene="gatB"
                     /locus_tag="NOA01_001436"
     CDS             11633..13063
                     /gene="gatB"
                     /locus_tag="NOA01_001436"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HCI0632634.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            13073..14128
                     /locus_tag="NOA01_001437"
     CDS             13073..14128
                     /locus_tag="NOA01_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCI0632635.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            14239..15612
                     /gene="rlmD"
                     /locus_tag="NOA01_001438"
     CDS             14239..15612
                     /gene="rlmD"
                     /locus_tag="NOA01_001438"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCI0632636.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            15615..15743
                     /locus_tag="NOA01_001439"
     CDS             15615..15743
                     /locus_tag="NOA01_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCI0632637.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            16167..16355
                     /locus_tag="NOA01_001440"
     CDS             16167..16355
                     /locus_tag="NOA01_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCI0632638.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            16433..17446
                     /locus_tag="NOA01_001441"
     CDS             16433..17446
                     /locus_tag="NOA01_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCI0632639.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(17513..18547)
                     /locus_tag="NOA01_001442"
     CDS             complement(17513..18547)
                     /locus_tag="NOA01_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCI0632640.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
BASE COUNT         6233 a         3432 c         4011 g         4897 t
ORIGIN      
        1 tattatttgt attgaggtga tcgtgaaaat gcttacagag gaacgtagac aaaaaatcct
       61 agacttgctt gaacagcaga aaatcgtgaa atcacaagat cttgtaaacc tttttaatgc
      121 ttctgaatca acgataagac gtgatttaca agagctggag aatagtgggt ttctagaaag
      181 agttcatggc ggagctaaga aagagcaatt attgaatttt gagcagaata tgagtgaaaa
      241 atcactcaaa aacgttcacg aaaaacagca aatcgccaaa tcggcagctg aaaccattaa
      301 tgacgaagat gtcatttatt tagacgctgg ctctactacg ttagaaatga ttccttttct
      361 gcaaggaaaa cagatcacag tagtgacaaa ttccgttcaa catgcggcaa atttagttga
      421 tttggagctt gctaccatta ttttaggtgg aacgataaag ctggcaacta atgctgtttt
      481 aggatctaat gcgatagaac aactaaaaaa ttttcatttt aataaagcat ttatgggaat
      541 gaacggtgcc catcttgaac aaggattcac gactccggac ccagaagagg cagctgttaa
      601 acgtttagcc atgaaaaatt ccgaagagac atttgtattg attgatcata ccaaattcaa
      661 ccagttgtcc ttcacctctg ttgcaccttt ggaagcagcc acgatcataa ccgagcgttg
      721 tccattagaa ttcgttaatg aattccgtga aaagacaaca attaaggagg caatacaatg
      781 atttacacag taacacttaa tccatcaatt gattatatcg tacgcgtaga tgggttaaaa
      841 ctaggcgatt tgaaccgaat gaaggaagac ttcaagttac ctggaggaaa agggatcaat
      901 gtttcccgta ttttgaaacg gatcgattct caatctacag cactagggtt tttaggaggg
      961 ttcactggcg aatttatttc gaagtggctt gaaaaagaac aaatccagtc tttgttcaca
     1021 accgtaaaag aagatacaag aatcaatatc aaactaaaat cagatactga aactgaaatt
     1081 aacggtttgg gcccatctat ttcagatgaa gaaatcgatg agttgaagca agcattagga
     1141 acagtaggac aaggagatat cgtcgtattg tcaggaagcg cgccggctag cctgcgaaaa
     1201 ggattttatc aagaactgat tgatatcatt cgagaaaaag gtgcagattt cgtgattgat
     1261 acaacaggtg atgacctaaa aaatgcactt gttaagaaac cattgttgat caaaccaaac
     1321 aatcacgaat tagctgagct ttacaataca accttccaat ctgtagaaga aatcattccg
     1381 tatggtaaaa aattgctagc tgaaggggca cagaatgtgc tgatctcaat ggctggagac
     1441 ggtgctttgc tcttcacgga acaaggaact tataaaacga atgtattagt tcgcccattg
     1501 aaaaattcag taggtgcagg ggattcaatg attgccggct ttattggggc ttacagccgt
     1561 acgaaagatc caatcgaagc ctttaaatgg ggagttgctt gcggaagcgc aacagctttt
     1621 tctgatgact tggcagctcg cgaattgatt gatgagttgc tgccagaagt tgaaatcaca
     1681 aaaattgatt agtttcaagg aggataatca tgaatatcaa ggatttaatg gtcaaagaag
     1741 cgatgatcat ggatttgcaa gcgactgata aaaaaggcgc aatcgatgaa atggtccaaa
     1801 aaatgtttga tgccggccgg atttccgata tcgaaacata taaagaagga attctagccc
     1861 gcgaagcaca aacttctaca ggtcttggtg atggaatcgc catgcctcac gcgaaaaaca
     1921 gtgcagttaa agaagcaaca gttttattcg caaaaagcca aaaaggagta gattatgaag
     1981 ccttagacgg acagccaact tatctgtttt tcatgatcgc agctccagaa ggcgcaaacg
     2041 acacacattt gcaagcatta gcagcactat ctcgattgct gattgatgca gatttcgtag
     2101 gaaaattaaa agaagcaaat acaccagaag aagtccaaga cttgttccaa acagctgaag
     2161 agcaaaaaga agcagaagaa aaagcagaac aagaaacaca agctgcagaa gcttctgctc
     2221 atccagaaag aaaattcgtt gttgccgtta cagcttgtcc aacagggatc gctcacactt
     2281 acatggcaga agatgctctg aagaaaaaag cgaaagaaat gggtgtggag atcaaagtcg
     2341 aaacgaacgg ttcagaagga atcaaaaacc gattgactgc tgaagacatc gcccgtgcag
     2401 aaggtgtaat cgttgcagca gacaaaaaag tagagatgaa tcgctttgac ggaaaagaac
     2461 tgatcaatcg tcctgtcagt gatggtatcc gtaaaccaga agagctgatc aatctagccc
     2521 ttagtggtac tgcaccagta ttccatggtg atggaaaaga aagcagttca gaagaaggaa
     2581 atgcggatgg tactatcgga caaagaattt acaaagattt gatgaacggc gtatcacaca
     2641 tgttaccttt cgttattggt ggaggaatcg ctattgcgct ttctttcatg atcgaccaat
     2701 ttattggcgt gccacatgat caattatcaa atctagggaa ttacaatcaa gctgctagct
     2761 ggttcaacca aatcggacaa gctgcatttg gtttcatgct gccggtatta gcaggattta
     2821 ttgcttcaag tatcggtgac cgccctggtt tgatcgttgg atttgctgct ggtgcattag
     2881 ctaatacagg cggtgcagga ttcttaggtg cactgattgg tggattctta gctggttatg
     2941 tcatcgtatt tttacgtaaa ttattcaaga acttaccaaa atcattggat ggaattaaaa
     3001 cgatcctctt ttatccagtg tttggcttac tgattactgg tttcttgatg ttgctggtca
     3061 atgtccctat gaaagcaatc aatgatgcat tgaacggttt cttgacaagt atgagtggtt
     3121 cgaatgctgc tttacttggt gcattattag ctggaatgat ggcggcagat ttaggcggtc
     3181 caatcaacaa agccgcttat gtgtttggta cagcaacttt agctacgact gttgcaactg
     3241 gcggaagtgt cgtgatggct tctgttatgg ctggaggaat ggtacctcca ttggcaattt
     3301 tcgttgctac acgtttgttt aagaataaat tcagcaaaac agatcaagat gctggtttga
     3361 cgaacatcgt gatgggacta tcgtttgtca cggaaggatc gattccattt gcagcagctg
     3421 acccgattcg tgccattcca agttttatta ttggttctgc tttaactggc ggattagtcg
     3481 gagctttcgg gatcaaatta ctggctcctc atggcgggat tttcgttgta ttcttactga
     3541 gtcacccggt catgtatttg gtgtttattg ctatcggagc agtcgtatct ggtgtgattt
     3601 atggtttatt gagaaaagct cctgcagcag cagaagcata gaaataaaaa agaagaggct
     3661 ggtttttccc agtctcttct ttttttatag aatcatatag aatcaagtag tgtagatgaa
     3721 tagttgttgt aaaatagttt ttccatgttc gttaaaatga ttgcttcaaa tttgtgatag
     3781 aagatttagc ctcgatttcc tgcttgattc ttggcaagtt tgcttcatca gtcaagataa
     3841 ccaaaatatc tcctacattc atgatcgtat caccgtgagt caagatttcc tgatttcctc
     3901 tgcgtataga gattagcagc atatttttag gccaattgaa gtctcgtacc attaatccgt
     3961 ctaaagaact ctctgccgtt actggaaaat caaaagttgt ccttgcacct tttagtgagg
     4021 cggtctgagt atttccaagt agacgttcca ataatgattc gtaaattgga ttaccgccga
     4081 gaaggtcatt gacgatataa gcaacaagag cagccaatcc taaaggcatc agatgattaa
     4141 gagagccaac catttctgtc actaaaataa ttgctgtcag cggtgctttt ccaatcgctg
     4201 tgaaataacc agccatggca ataacgacaa aatccttgat gtagattgga tccatcccga
     4261 aatcaaccat aatgtttcca taaaagctac cgattaaagc ccccaatgtc aatatcggaa
     4321 ggaaaatccc ccctggaaga ccaccaccgt aagaaatcat ggaaaaaacg aatcgaagaa
     4381 caaacaaacc gataatcata atgattggca aggaagcatg tccaaaagct aaaacgattt
     4441 gattgcctcc gcctagagta ctaggccata aataaccaat tggcaaaatc agcaagaaag
     4501 gaacgaaccc gtgaaaataa gctggtaaag gcaatcggtt gtaaaatttt ggcaaagcca
     4561 gcaagacctt ctgataaata aaacctaaga tgcctagaag tactcctaag atcactaagg
     4621 aaccataata tttcaacggc aaagaaggaa ggtcacctag atataaaaca ggtctcaagc
     4681 cgaaaaagta aagagagacg aagttagcag tgattgctga agcaaatgat gtcagccaaa
     4741 caagtggaga aaaggagtga tggatttctt ctaaaacaaa gagtaatcct gcgataggag
     4801 cattgaatgc tgctgcaagt ccgctacttg caccgctaga aattaaaatc ttttcttctg
     4861 aagatggact ccgaaacaat tgtccagctc cttgtccaat ggatgcgcca agctgtatag
     4921 aaggtccttc tctacctaga aaaaggcccg caccaactga aagtacaccg ccaatgaact
     4981 ttttccataa aacagagaac cagttcattg ataattcccc gcgaagctgt ccctctactt
     5041 gtggaatccc gcttcccttg atatccggtt catttttgac cagataaccc acgaaaaatg
     5101 ctgcaaggat gcagcaaata gctaggccaa ttaagtaaat cggctgttcc cgactcattt
     5161 catagatact tcgaactaaa tcgcttagct tttcgataga taatcgaaac aggctaacga
     5221 cgattcctgt cagcagtcca acaaatattc cttttagaat aaaatatatc tgagtcttat
     5281 ctaatttctt gatttttcct tttttttcca ttcaaacgcc tcgctttctt ttaatttagc
     5341 gcgaaatcaa cgaattgaca aggaaaaatc cataaaaaga aacaaatgat tttgaactag
     5401 ctagtttaat gaaagccaag aaaatgctat aattaacaag attgataaaa gaaaggatgt
     5461 tgttaatgtc atccaaagct gaattattaa acgggatgaa cccccgtcag aaagaagctg
     5521 ttcttcacac tgacggacca ttactgctaa tggcaggtgc aggaagtgga aagacgagag
     5581 tgctgacaca ccgcattgcc tatttgattg aggaaaaaga agtcaatcct tggaacatcc
     5641 tagcgattac ttttaccaat aaggctgcaa aagaaatgaa agaacgtgtc aatgcgattc
     5701 tcgcatctgg aggcgaagat gtctgggtat cgacttttca ctcgatgtgt gtgcgtattt
     5761 taagaagaga tgttgatttt atcgggtata accggaactt tacaatcatc gattcttctg
     5821 aacagctgac cttgatgaaa cggatactaa aagaactgaa cattgatccg aaaaaatatg
     5881 atcctcgaag tatattagga acaatctctc aagcaaaaaa cagcctgcag acccctcagg
     5941 attttgcaaa aatgcaaggc agttattacg aagagattgc ggcaaaatgt tatgctgctt
     6001 accaaaaaga gcttcaatat aatcaatgta tggactttga cgacctgatc atgaatacga
     6061 ttcgattatt tgaagaacat cctgattcac tcacatatta tcaaaacaaa ttccattata
     6121 ttcatgtaga tgagtaccaa gatacaaatc acgcacagta tacgctagtc aatttattgg
     6181 caggtagatt taggaacctg tgcgtcgtgg gagatgcaga tcaaagtatt tacggctggc
     6241 gcggtgcaga tatgcagaac atcttggatt ttgaaaaaga ttatccagat gctgcagtaa
     6301 ttttgctaga acaaaactat cgctccacga aaaacatcct tagcgcagca aaccaagtca
     6361 ttgaaaacaa tagcaatcga aaaccaaaaa acttgtggac agaaaacaaa gaaggaaaca
     6421 agatcactta ctaccgtgca gataatgaac gagacgaaac ccgttttatc gtagatcgga
     6481 tgcaagaaga gattcgaagc aatcatcgga attacggcga ctttgccatc ttgtaccgga
     6541 cgaacgcaca atctcgtgta atggaagaaa ctctgttgaa agcaaatatt ccttataaaa
     6601 tggttggagg gcacaagttc tacgatcgta aagaaatcaa agatatttta gcgtatttga
     6661 atgtgctggc aaatcctcaa gattcaatca gttttgagcg tatcgttaat tcgcccaaac
     6721 gaggcatcgg accaggatct atcgaaaaac tacgcagttt tgcttctctt catgaatggc
     6781 cactgcttga agcagcgcaa aatgtggatc tggcaaatat cggtggaaaa gcaggacagc
     6841 aattaggtgc atttggagag atgatccaag aagtcactca gatgatccca tacttgacag
     6901 tgacagaatt gacgaaagag gttttggatc gtagcggata tctagaagac ttaaaaatcc
     6961 aaaatacatt agaagcacaa gcacgcatcg aaaacttgga ggaattttta acggtcacac
     7021 aagaattcga caaacaattc gaacagcaaa atgaagaaga cgctgatgca ccagaagaaa
     7081 aactaacggt cttcttaaat gatctggcac ttgtttccga tattgacaat ttagaagaag
     7141 atgcttccca agtgacattg atgactttgc atgcagccaa aggattagaa ttcccagttg
     7201 tattcctgat tggtctagaa gagggggttt ttcctttatc tcgagcattg atggaagaaa
     7261 gcgaactgga agaagaacgc cgtctcgcat atgtggggat cacgcgagca gaagaagcgt
     7321 tgtatttaac aaatgctttc tccagaacat tatatggacg gacgcaatat aaccgacctt
     7381 ctcgttttgt agaagaaatc gatcaggagt tactggaaat cgaaggcatg cgtcctacgc
     7441 ctaaaaaaac acctgtattc gctaaaaaaa cagcatatag ctataaacag ccagagacag
     7501 cggttgttcc ctctaaaagt gctactggag gggaaaataa cagctggaaa cctggagata
     7561 aagtcaaaca caaaaaatgg ggtctaggaa cagtcgtccg agtaagcggg acgtcgaagg
     7621 atctagaact agatgtggct ttcccaagtc aaggagtaaa acgtcttttg gctgcctttg
     7681 cgccaattga aaaagcttag tagtggaggg taaatgatgg aacatgaaat gacgattgac
     7741 gaagctgcta aacgagcaga agaacttcgt acaagactga accaatggtc tcgcgaatac
     7801 tacgtagaag ataaaccaac agtggaagac tatgtttatg ataaagaata cgcagaatta
     7861 gtagcaattg aggaacaata tcctgatttg atcacgtctg attctcccac tcaacgagta
     7921 ggcggaaaag tattagaagg ttttgaaaaa gtcactcatg atattccgct ttacagtttg
     7981 aacgacgttt tcagcaaaga agagctgata gcttttgatc aacgtgtaca aaaagcagtg
     8041 ggacgtgtag ttgactattg ctgtgaacta aaaatagatg gactttccgt ttctttgcgt
     8101 tatgaagatg gaaatttcgt gagaggtgcg actcgcgggg atggaactgt aggagaaaac
     8161 atcactgaaa acctaaaaac tgtccggtcg gtcccaatca aattaaaaga accaatgaac
     8221 atcgaagtaa gaggcgaatg tttcatgcca aaacggtcat ttgtccaatt gaaccaagac
     8281 agagaagcag agggaaaaga tatctttgcg aatcctcgga acgccgcagc tggttctttg
     8341 cgtcagttag attctaaaat cacagcgaaa agaaatctgg acactttttt atacacagta
     8401 gctgatttcg gtcctatgca agcaaaaacc cagtatgatg cgctagaaga gctagaaaaa
     8461 attggattcc acacgaatcg ggaaaaacgt ctatgtcatt cgattgatga agtgtggtca
     8521 tacattgaag aatatcatga caaacgtgtg gatttgccct atgaaatcga tggaatcgtc
     8581 atcaaagtga atgaattctc gctacaagat caacttggtt ttactgtcaa agcaccgaga
     8641 tgggcagcag cttacaagtt tccgccagaa gaagttgaaa cgctaatcga aaacatcgaa
     8701 tggacagtcg gtcgaacagg ggtagtaacg ccaacagcta tcatgacacc agtccgagta
     8761 gcaggaacga cagtaagccg tgccagcctg cataacggtg attatattaa gttgaaggat
     8821 atccgcttga aagatacagt tctgatttat aaagcgggag acatcatccc agaagtttcg
     8881 caagtcgtgt tagataaacg acctaaagac agcgaagaat accaattgcc tacacattgt
     8941 ccagtttgtg gaagtgaact tgtccatttg gatgaagaag tagccttacg gtgcatcaat
     9001 cctaaatgcc ctgctcaaat gaaagaaggt ttgaaccact ttgtttcaag aaatgcgatg
     9061 aatatcgatg ggttggggcc gcgcgtacta gaacagatgt atgataaaaa actagtagca
     9121 gatgttgctg atctttacaa attgacagaa gaagagttat tgacgttgga caaaataaaa
     9181 gaaaaatcag caaataatat tttgacagca atcgacaaca gcaaagataa ttcggtcgag
     9241 cggttgattt tcgggttagg aatccgacat gtcggagcga aagcagcaaa aattttagca
     9301 gaacatttcg gtgatctaga aacattgagt aaaagtgact atgagtcgat tattgcgctt
     9361 gatacgatcg gtgatatcat tgccgacagt gtagtcactt atttcagtaa cgaagaagtc
     9421 catgaattga tgaatgaatt aaaacaagca ggcgtcaatt tcgaatacaa agggcttcga
     9481 aatgcccaat tgcaagaagt cgagtctcct tttaaagaaa aaacagtagt ccttactggt
     9541 aaactaacac gatttactcg tgaagaagca aaagagacga tcgaaaatct tggcgggaaa
     9601 gtgactggta gcgtatcgaa aaaaacggat atcgtggtag caggagaaga tgctggcagc
     9661 aagctgacca aagcacaaga attaggaatc gaagtttgga cagaagatca aatggcagat
     9721 gcattggcta aaagtcgctc agtagaagaa tgaaaaatga gttttttcag acaaatttca
     9781 ataaacaatt tcagacacgg cactcttgtg ttaaaataga gagcaaagtt tttaactcgt
     9841 ttagaaagaa ggggtagtat ggcgattagt gaagaacaag tgaaacacgt tgccaaatta
     9901 gcaaaacttg cgttcgcaga tgaagaactt gcaagtttta ccgaccaatt aggtaaaatc
     9961 atcgatatgg ttgaaatgct ggaagaagta gatacagaag gtgttccatt tacgtcgaat
    10021 gtggttgaaa cagtaaacgt gatgcgacaa gatcgggcgc aagctggatg gaaccgcgat
    10081 gaattattga aaaatgtgcc ggaaacagaa gacggcttta tcaaagtacc cgcaatcatc
    10141 gataatgggg aggctggcgc ataatgacag aattacacaa tcgttctcta gaagaactgc
    10201 atcatatgct tgtatcgaaa gaaatcactg tacaagattt gacaaaagca acatttgaac
    10261 gaatcaaaga aacagagcct gaggtcgaag cattcattac attgaacgaa gaaaaagcat
    10321 tggaacaggc aaaagcttta gatgaaaaag ggattgatga aagcaacgtc cttgctggta
    10381 ttccaattgg tatcaaggat aatatcgtaa caaaagatat cttgacaacc gctgcatcta
    10441 agatccttta caattttgaa ccaatttatg atgcaacggt catggataaa atctatcaag
    10501 cagatatgat ccctgttggt aagctaaaca tggatgaatt tgctatgggt ggaagtacag
    10561 aaacttctta tttcaagaaa acaaaaaatg cttgggaccc aacaaaagta cctggtggct
    10621 cttctggtgg ttctgcagca gcagttgcag ctggacaaat tccagtttct ttaggtagtg
    10681 atactggggg aagtatccgc caaccagcag cttttaacgg catcgtaggg atgaaaccta
    10741 cttatggccg tgtctcacgt tttggtttga ttgcctttgc ttcatctctt gaccaaatcg
    10801 gaccaatgac ccgtacggtg aaagataatg cattagcatt gacagctatc agcggttatg
    10861 acgaaaaaga cggtacttct tcaggcgttt cagttcctga ttttgcagag ggactgacag
    10921 gcgacatcaa aggaatgaag attgctttac ctaaagaata cttaggcgaa ggtgtggccc
    10981 ctggcgtaaa agaagctgta ttgaaagcag cagaaacatt ccgttcatta ggtgctgttg
    11041 tggaagaagt cagcttgcct cattcaaaat acggtgtggc tgtttattat atcattgcct
    11101 catcagaagc aagttccaac ttgcaacgat tcgatggtat ccgctacggt tatcgctcag
    11161 aaaatgtgaa atcattagat gatgtatatg tcaactcacg ttcagaagga ttcggtgaag
    11221 aagtaaaacg ccgtatcatg ttaggtacat tctccttaag tgctggttat tatgacgctt
    11281 acttcaaaaa agcgggacaa gtccgtacat tgatcaaacg agattttgaa aaagtctttg
    11341 cagattatga cttgatcatc gggccatctt caccaacagt tgcattcgga atcggtgaga
    11401 acattaacga tccaatcaca atgtacatga gtgacatctt aacgatccct gtcaacttgg
    11461 ctggacttcc aggtatgtct gtaccggcag gtttatcaga aggattacca gttggattgc
    11521 agatcatcgg taattatttt gatgaaaaaa cgatgtatca agcggcatat gcgtttgaac
    11581 aagcaacaga attccataaa caacaaccag ctattttagg agggaaagac gaatgaattt
    11641 tgaaacagtc attggactag aagtccacgt cgagttaaaa accaactcaa aaattttctc
    11701 tccagcgcca gctcattttg gtgcagaacc aaacagcaat acaaacgtga tcgactgggg
    11761 ttatccaggc gtgctgccag taatgaataa agctgcgctg gaattcggga tgaaagcagc
    11821 tttggcatta aactgtgaaa tctctaaaga tactcatttt gaccggaaaa attactttta
    11881 cccagataat ccaaaagcat atcaaatttc ccaatttgat caaccaatcg gtcatgacgg
    11941 atggatcgaa atcgaagtag aaggtaaaaa gaaaaaaatc cggatcgaac gtgtccattt
    12001 agaagaagat gctggaaaaa acatgcatgg tattggcggt tattcttacg ttgatttgaa
    12061 ccgacaaggc acgcctttga tcgaaatcgt ttctgaagct gatatgcgtt caccagaaga
    12121 agcatacgct tatctagaag cattgcgatc aattatccaa tttacagaag tcagcgatgt
    12181 gaaaatggaa gaaggctcta tgcgctgtga tgccaatatt tctcttcgcc cttatggaca
    12241 agaagagttc ggtacgaaag cagaattgaa aaacttgaac tctttatctt tcgtgaaaaa
    12301 aggtttagct tttgaagaaa aacgtcaagc aaaagttctt ctttcaggag gagaaatcca
    12361 acaagaaaca cgccgttttg acgaaacaac gaataaaaca gttttgatgc gtgtcaaaga
    12421 agggtctagt gattatcgtt acttcccaga gcctgatatc ccgaaattcg tgatcgatga
    12481 cgaatggatt caaaaagtaa aagctagttt gcctgagatg ccagcttctc gtcgcgaacg
    12541 ctatatccgt gagtttggac ttccagagta tgacgcaatg gtattgacac ttacaaaaga
    12601 aatgtctgat ttttttgaag ctgttttgga aaatggtgca gatgccaagc aagcatctaa
    12661 ctggttgatg ggtgaagttt ctgcttactt gaacagtgag aaactggaat tagctgagac
    12721 aaaattgaca cctgagaact tagcaggaat gattcgtttg atcgccgatg gaacgatcag
    12781 ctcaaaaatc gccaaaaaag tcttccgtga attgatcaca aatggcggag aagcccaaga
    12841 agtagttgaa aaaaatggtt gggtgcaatt atctgatccg aacaaactac tgccaatcat
    12901 taatgaaatt ttggataaca accaacaatc tgtcgatgat ttcaaaaatg gaaaagatcg
    12961 tgcggttggc ttcctagtcg gtcaaatcat gaaagcaact aaaggacaag caaatcctgg
    13021 tgtcgtgaat aagctactta aagatgaatt agcgaaacga tagaggctga aaatgaaaaa
    13081 agcacgcgta atttataacc ccacctcggg aaaagaatta ttgaaaaaaa atttggcgga
    13141 tatcctacag gcattagaag aagcaggatt tgaagccagc gcctatgcaa ctacaccaga
    13201 accagattca gccaagaatg aagcatgtag agtcgcagaa ctaggttttg atctagtagt
    13261 ggcagctggt ggcgatggca caatcaacga agtggttaat ggaatcgctc cgcttgaaca
    13321 ccgtcctaaa atggccatca tcccagctgg tacaacgaac gactatgctc gagcattaaa
    13381 aattcctaga gataatatta aagcagcagc agaagtgata aaaaaacacc aaaccgtaaa
    13441 aatggacatc ggcctttctg ataacagtta cttcatcaat atcgctgcag gtggctattt
    13501 gacagaattg acttatgaag ttccatcaga actaaagagt atttttggtt atcttgctta
    13561 tcttgcgaaa ggagcagaga tgctccctcg agtaaagcca atcaaaatgc gcttgaagta
    13621 tgatgaaggg gaatatatcg gaaatgcttc gatgtttttc ctagggctga cgaactctgt
    13681 tggaggattc gaacagatcg cgccagatgc caaattggat gatgggaagt tttctttaat
    13741 cattgtgaag acagcaaata tctttgagat cttgcatatt gcagcactca tgctgaatgg
    13801 cggtcgacat gtcgatgatc caagaataat ctataccaaa acaagcagtt tgttcgttga
    13861 aacagaagat gatatcaatc gaccagtaat gatcaactta gatggtgaat acggcgggga
    13921 agcaccgatg catttccaaa atcttcatca acacattgag ttctttgcca ataccgatca
    13981 gattcctgat gaagcaatca caggtacaga tgaagaagaa ttagaggttg tcagcaaaga
    14041 attcgtaaaa gaagtcgaac gtttaacgga tgaagatatc gacggtgacg gaaaaatagc
    14101 agacgaaaca cctaatcaaa agaattaatc aaaagccggt gctgaacgta gtaaggtcag
    14161 tgccggtttt agttgtgtaa ttagctgatt tatagtagag ttcagtagga ttgaaaaata
    14221 ggcttaaagg agcaaaatat gacagaacag caaatagtaa agaaaaacga acgatataca
    14281 gtcgaaatcg tcgacttgag ttatgaagga atgggggtag caaaaatcga tggttatcca
    14341 ttatttatcg aaaatgcact ccctggagaa gaagtagaaa tactagtagt aaaagcaggc
    14401 aataagtttg gatatggaaa agtcgaaaca tttattacac aatcacctga tcgtcagcct
    14461 gtagaaaaca atgcattact tcgcataggt atcgcacctt tagcccattt gaagtatgaa
    14521 caacagcttg tcttcaaaca aaaacaagta gagcatgtaa tggcgaagat tgctaagatg
    14581 ccagaagttc ctgtacttcc aacggtcgga atggcagaac cattcggcta ccgtaataaa
    14641 gcacagatcc ctgttcgccg tatcgatgga cagttagcca ccggttttta caaaaagaat
    14701 agccatgatc tagtccctat cgaagatttt tatatccaag atcctgtcat cgatcaagca
    14761 atcaaagtag tcagagatat cttgcaacgg ttccaagtac gaggatataa tgaagaaaaa
    14821 aatgaaggac agcttcgtca catcattatc cgtcgtggtc attattccca tgaaatgatg
    14881 gtcgttttag ttactcgtaa agaaaaattc tttaaatcca aggaaattgc tgaagctatt
    14941 catgaagaat tgccagaagt ggtttcggtc atccaaaata tcaatgaaga aaaaacaaat
    15001 gtgattttag gagacaaaga aaaagtttta tatggcagag actatatcga agatcagcta
    15061 ttaggaaaaa cctatcgaat ctcagcaaag tcattttatc aagtaaatac tcaacaagca
    15121 gagtatctgt accaaacagc catcgagttt gccgagttag ataaaaaaga cattgtgatc
    15181 gacgcttatt caggaatagg tacgattggc ttgtctttag cagacagagt ggataaggtg
    15241 tatgggatgg aagttattcc agaagcaatt gaagatgcac agttcaatgc tttaaataat
    15301 aaaatcgaaa atgttcatta tgaagtcgga aaagcagaaa aagttatgaa aaaatggcaa
    15361 gaaaaaggga tcaaaccatc tgtgatcgta gttgaccctc cacgaaaagg attagatcat
    15421 cattttattg aatcagcaat cgaaatgtca ccagaacgaa tcgtctatat ctcatgtaat
    15481 ccggcaactt ttgcaagaga tgctaagtta tttgcagaag caggttatgc cgtgaagaaa
    15541 gttcagcctg tagatctttt cccgcaaacc catcatatcg aattagtcgc ttcattcgtt
    15601 aaaggagaat aaaaatggac caaaaagaaa aaatgaaaga attgatcaaa aagaaacaag
    15661 gtggaggccg atccaaacaa atggaaacac ctaagaatga tcgtaaaaac atgagaaaag
    15721 gccctaaaat ctttaataaa tagacgtgct aaagaaagac taagaagtgt taacttttgt
    15781 tattgtttct tagtcttttt agtggctctt tgtcaataag gactgatggg ttgtacaaaa
    15841 tcaaatctgg gtcagaatga acctcattct ggctcagatt tttcgttatt tcaatttgat
    15901 taactgattg atcaaaaaga tttttatttt catattctca aatgattcaa cactaaatat
    15961 acatgtttga cgcggaaatt attattcgat tttggcggca tcagggggga tgacgacgta
    16021 gcagacccat tattttactc aagaaaatgt ccgtgcctat tatttagaat acgatagtga
    16081 acgtgctggg agttttgttc ttttagagaa agtctctgaa gataaattag ttgtcttaga
    16141 ttttgactgt aacgaaaaat ggcgaaatgg aagagcacca gcaagtgatt gaccggatct
    16201 acgaagcgac acgatatctg cctttggatc gtttgtgctt aagtcctcaa tgtggatttg
    16261 cttcaacaga agaagaaaat gttttgtttg aagaacaatg gaaaaaattt cttttgtcaa
    16321 aagcattgca gaagaagttt ggggaaaatc attaaatcat ctaggagagc ttgataggaa
    16381 ataacactat ggtataatca gaatgcttaa tactaactac tgagggagcc ttatgaaaaa
    16441 gaaaattctt aaatttatcg gatggttctt cggaatcatt cttgggatcg cgtttgcaat
    16501 ctttcttgcc ttccagcttt cccccagacc tggagcattc ttgatcaacc atatgttttc
    16561 aaatactgtt caaatcactg ataaaaaaac gttcgatcaa gcagaaaaaa acgtggcgaa
    16621 aaaaacagat ttgacgtatg agtccaagta taaagagaat acttatgaca tctactatcc
    16681 aaaaaacagc aaggggcctg ttcctgttct attttgggta catggtggag gattcgtcgg
    16741 cggagataaa tcgggtgtca aagaatttgc gacaaaatta gtttcagacg ccaacatcgc
    16801 agtggttgcg atgaattatg aattagcgcc tgattcagaa taccctaatc aagttcttca
    16861 agtgaatgaa ctgatcaaag atttattagc tgaagaaaaa gacgacaaat tgctagacat
    16921 ggaacaatta ttttttggtg gagatagtgc aggttcacaa atcgcattgc aatatgcagc
    16981 tatacaaacg aatgatgcgt atgctaaaca aatgaaaatc aaacagctac ttccaaaaga
    17041 tagtctgaaa ggaaccattt cttattgcgg acctgttgat ttgaagctaa cagcacagca
    17101 gcattcggat gatcgattta tgaagttttt cgtcaaaacc gttgcgtggt cgttgattgg
    17161 aacaaaagat tggaaaacga gtgagcaatt gaaagaagct agtgtggctg accatgtttc
    17221 taaggaattt ccgccaacgt atataactga cggaaatgca tactcattcc aggagcaagg
    17281 tcaagcatta gaaaatcggt tgacagaatt aaaggtaccc gtgcaatcct tgttttatac
    17341 agatacaaaa aaagagatta cccatgaata ccaatttgat tacacgctaa aagaatcgca
    17401 aaattgttac cagcagacat tggattttgt gaataagtat aagtaagagg ttgtgaaaaa
    17461 gccgtttcgt tttgagtatg aagaaacgaa gcggcttttc acaacctctc tttcaatacc
    17521 tttttgttct tgggataaaa ctttccctaa caatcaatcg tgcaggataa gtcaaaatga
    17581 ttggttcgtc ccgtataccg tcaattcgct ccaatgccat tcgaacagct gcttgcccaa
    17641 ttgatttcgc attgatttga acactggtca agccagggga gaggtttgcg attgtctcga
    17701 tattatcaaa gcttgctaca acaatctctt ctccaatttg gatatttttt tgtttcaatg
    17761 cccgatagac accaattgcc attggatcgc tcccgacaag aatggcagta ggaagagggg
    17821 attcattttc taatagatta gtcgcaagta cctcaccttg tttttctgtc cagccaccta
    17881 gatgataaga catatatttt tctaagtgat gatcgatcat aaaatgctga taggcacgca
    17941 tcctcttttc atttatagaa atctgcttcc caccgaattc atccatatcg actttatatc
    18001 ccccgatata ggctattctt ttatgaccat tttctaagaa caattccagt acggttttag
    18061 tcatccgttc aagatctgca tacaccatat cgacctcttg ctggatgtca gggttatcca
    18121 cgacgactat ttgcatgttt tgttttaaca aggcggtaat cgattctttc gtaacagtcc
    18181 caacaacgat caccgcacct aactgatcta gatcttccca ttgtttagga ttatcagata
    18241 gattatagat tcgattcatc cctaaatgca gacgtcgagc ttcttcttcc atccctactc
    18301 gcaaatgttt gtaatagata tcctgttctt cgctcgcttt gccgatcgta gagatcacac
    18361 caatcgaagg cattctcttt ttgatatatt tacgagtact gatgtcataa tccaacgcag
    18421 cagctgcctc tactatccgt tgccgagtag tctgagccac atggatcgtc tgatcgttat
    18481 tcaacacacg ggaaactgta gcaggagaga catttgcctt tcgtgcgaca tctctaattc
    18541 caaccatttt tacacactcc tcaaaaaaat aaa
//
LOCUS       DAIGOK010000053        18053 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.053, whole genome shotgun sequence.
ACCESSION   DAIGOK010000053 DAIGOK010000000
VERSION     DAIGOK010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18053
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.053"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(<1..815)
                     /locus_tag="NOA01_001443"
     CDS             complement(<1..815)
                     /locus_tag="NOA01_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCI0632641.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEI"
     gene            complement(1119..3284)
                     /locus_tag="NOA01_001444"
     CDS             complement(1119..3284)
                     /locus_tag="NOA01_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCI0632642.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            3544..5838
                     /locus_tag="NOA01_001445"
     CDS             3544..5838
                     /locus_tag="NOA01_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HCI0632643.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTTHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5831..6496
                     /gene="pgmB"
                     /locus_tag="NOA01_001446"
     CDS             5831..6496
                     /gene="pgmB"
                     /locus_tag="NOA01_001446"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCI0632644.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            6489..7496
                     /locus_tag="NOA01_001447"
     CDS             6489..7496
                     /locus_tag="NOA01_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCI0632645.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            7518..8537
                     /locus_tag="NOA01_001448"
     CDS             7518..8537
                     /locus_tag="NOA01_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCI0632646.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(8818..10185)
                     /gene="nhaC"
                     /locus_tag="NOA01_001449"
     CDS             complement(8818..10185)
                     /gene="nhaC"
                     /locus_tag="NOA01_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCI0632647.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(10294..11715)
                     /locus_tag="NOA01_001450"
     CDS             complement(10294..11715)
                     /locus_tag="NOA01_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCI0632648.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(11958..13835)
                     /gene="tdc"
                     /locus_tag="NOA01_001451"
     CDS             complement(11958..13835)
                     /gene="tdc"
                     /locus_tag="NOA01_001451"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HCI0632649.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(14126..15382)
                     /locus_tag="NOA01_001452"
     CDS             complement(14126..15382)
                     /locus_tag="NOA01_001452"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCI0632650.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(15494..15699)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15962..17263
                     /locus_tag="NOA01_001453"
     CDS             15962..17263
                     /locus_tag="NOA01_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCI0632651.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(17332..17931)
                     /locus_tag="NOA01_001454"
     CDS             complement(17332..17931)
                     /locus_tag="NOA01_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCI0632652.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
BASE COUNT         5495 a         3828 c         3267 g         5463 t
ORIGIN      
        1 tcgatttcca ccccgtagtg atcactgatg attggactat cgatcccatc aaaaatccgt
       61 tgatattttt taacctgcat tccttcaggg acgaaaataa aatctatacg caatttttct
      121 gtattttgtt cccaaccatc tattttcttt tccacagtag ctgcttttcc tttttcattc
      181 gcgacaacat acgaatcttg aagaagaagg caactttctc ttacagtata ataaccttga
      241 gagtctactg gattattgaa atcacctagg aaaaaaagtg gtacgcgagt ttccaaaaag
      301 tagttttcca agtttttcca ctcaaaataa aagccacttt ttttatctgt ccaccaagaa
      361 aagtggcagc tcgagacaaa aatgtcctga tcatctatct ttgttttccc aatcaagatc
      421 ttgcgtgtct gataatcttc tttcttttga aattgggaga cggtaaggac ctgactttcc
      481 aatgtttttt tcgtaagcag tgcatttcct tcttcatatt tatcataacc gatgtggctc
      541 atttcccaac tccaaaaata ctcttttccg tgttccttta aaagctgaac taaacggtag
      601 gcaaaattat ctttacggat aggtacttga ttctttaccg gacagaactt cattagcttc
      661 gcaggttcta cttcttcaga ttccagtaat tggttgattt cttgtaaagc aatcacttca
      721 taatcttcta acaggatctt atctgctaat tcttgcaatt ttttctctgg ttctggctca
      781 agccagctat gcgtgttgag tgtcatgaat ttcattggtt ttctccttgt cttcatttcg
      841 aaacaaaagg gttgtgacaa aatttttgtc acaacctctc cagcaaatag tgttctcctg
      901 ctaactcctc agtaataagc tcaatggacc aggagctatt ttttgtttgg ctgtttttat
      961 cacttggtgc agggcagctt tttcacgacc tctccactaa acagtattca aactgctgct
     1021 tctcggtaat aagcccaacc aaacgaaaaa taggaggagc tatttttgtt tggctgttct
     1081 tatgacttga tgcaggacag ctttttcaca acctctcctt acattttaat ggagccgatc
     1141 actttaccag ctgtcactgt acctaattgc ttgatcacta cttcttctac tttatcgttt
     1201 gtaaatacga tcatgatcgt tggatctttt tcttcgtttt ggatcacatc aagatccatc
     1261 tcagctaatt tcgttcctgt tcctacttct tggccttctt tgacagcaat ctcaaaagct
     1321 ggctgattca tttggactgt atcgattccc atatggatca aaacttcgat accatctttc
     1381 gtttccagcc caattgcatg tttgctaggg aaaatacttg tcacttttcc agcgatcggc
     1441 gcaacaactt ctcttgatgt tggaataaca gcaaagccgt cacccatcat tttttgtgag
     1501 aataccggat cttttacttc agataaagga atcacttctc cagaagctgc ggaaactagt
     1561 tcttgttcta tacctaagta ttgcttacta cctgcttgtc catttcctgt agaaaccatt
     1621 tctggctctg gaatctctgc tcctgattgc agcagatctt cgatatctga tttcaagaca
     1681 tctgcttttg gtccataaac tgcttggact ccattgtctt tgacgatcaa tcccatcgcc
     1741 cctgctcgtt tccaggcttc ttcagtccct actttttctc gatcatttac acttacgcgt
     1801 aaccgagtca tacaagcatc gacatctttg atattttctt ttcctcccag caaatgaatg
     1861 atctggacca cttgctgatc tgctgttcct gttccgctgt tagatacaac ggtttcttca
     1921 ctgtcattgt cgtaatttcc gttacgtccc ggtgtagcaa agttgaattt tttgatcaag
     1981 aaatttgcta agaaatacgt aacaacacca aaagcgatca cacacaacac gaagttgata
     2041 agatctccac ctagacccgc tttgatagct aacggtgtcc gagtcagcaa ttcgatattc
     2101 ccaaatgaat ggacccgcaa tggcaagata tctgccatcg caaatgcagc cccttggatc
     2161 actgaataaa tgacatataa tggcacagca gcaaacataa acatgaactc caatggttct
     2221 gttacacctg ttagaaagac agccaacgct gctgaaaaat acattgattt atatcttgct
     2281 ttcttatcag gatcgacatt acgatacatc gcaaaagcca ttcccatcaa gatacctgaa
     2341 gaaccaatca tttgtcctac tttgaaacga gccggcgtcc agttatgcaa cacaaactca
     2401 tattgcgaca tatcccctgc acctttaaga ttcactaaat cagtggccca agccaaccat
     2461 aacggatctt gcccaaatac ttgtgtcccc gcctgtgcgc ctgataaaat ctcatacgat
     2521 ccgccaagct gtgtatagtt gataggaatc gtcagcatat gatgcaatcc aaatggcaat
     2581 agtaaacgtt ctaatgttcc aaataaaaac ggtgctagaa tcggtgcgct ttcttgtgat
     2641 tctgcaatcc ataaaccaaa attattgatt ccagcctgta cgtttggcca caccacagca
     2701 agaatcaatg ccacgattgt tgaccataag ataacaacaa acggaacaaa gcgctttcca
     2761 ttgaaaaatg acaaagcatc cggcaatttt ctataattat agtacttatt ataagccatt
     2821 gccccgacaa atccagcgat gatccctacg aatactccca tattcaaagc tggtgcttcc
     2881 aatacacttg taaaaaatcc tttaaccatg atcttcgtac cgaacagagt gtgggtaaat
     2941 gcatcttcat tcgcaagcat gtcacttgtg acaccaaaga tagccccagt gatccgattg
     3001 attaaaataa atgagatccc agctgcaaat gctccgcctg ctctttcttt tgcccaactt
     3061 cctcctattg ctaaagcaaa taaaatatga agattaccaa taattgccca accaatattt
     3121 tcaattactc cgcccgttgt gacaagcggc gtccaatcag gattgatcaa gggaattgat
     3181 ttccctatac tgatcatcaa cccagctgcc ggcataactg ctaccacaac cattaaagcc
     3241 tttccaaatt tttgccaaaa ctcaaaacta aatatctttt tcattccgtt tagctccttt
     3301 acgcaatcgg tttcgttaaa cgtattatag cgtgcgtttt cttttttgta aatgctttat
     3361 tttttatttt tctgtctata ccattctata aaacctataa tacgtgtgtt cccttctata
     3421 attttctcct aaaaatttat ttttttaagc ttttttctat gaaatatttt cttttgtcta
     3481 ttgacttgtg caatcggttg cgtttattat gaacgtataa aacagctcat taggaggaga
     3541 aaaatgaaac agatcaaacg actatttgct attgatccat ggaaaatcag cacgacacaa
     3601 ttagataaaa aaaatcttcg actgcaagaa tctttaacaa gtattggaaa cggttatatg
     3661 gggatgcgcg ggaactttga agaaacttac tcaggggacc accatcaagg tacttatttg
     3721 gcgggtgtct ggtatcccga taagacacgt gtcggctggt ggaaaaatgg ttatccagaa
     3781 tactttggaa aagtgatcaa cgccttgaac tttatcgcga tagatatttc gatcaacgat
     3841 cagctgatcg acttggcttc tttggaacca gaagactttt actgggaatt agatatgaaa
     3901 aatggtgtat tatctcgtac attcacaatc actacttcta ctaataaagt acgcatttcc
     3961 ttcgaacgtt ttctgagcat tgtcaaaaaa gaatcagctt atatctattt aactatagaa
     4021 atgcttgaag gtagcggaac agtcaaactc gtatcaaaat tggatggaaa tgttcaaaac
     4081 gaagacagca actatgatga acatttttgg aatgagattg atcgtggaac tatagaagaa
     4141 atcggctatt tgacgactaa aactattcca aacaactttg ggattgaaca atttactgtt
     4201 actgcagcaa tgcgccattt cgttaatgga aaatcagtca tccctgacta tcacgaagat
     4261 actttagcat tacatggttg cttgtctgca gaattatctc aaggtgagac atggtcagtg
     4321 gaaaaagaaa ttatcgttct tactagtaga gatattccag aagcgcagca agcagaaaaa
     4381 gctgtctcag aattaacgaa ccagacaaac aattatatcc aagcaaaaga agagcaaaca
     4441 gcggcttggg cagaacgatg gaagcttgcc gatgtggtca tcgaaggaga cgaagaagca
     4501 caacaaggta ttcgattcaa tttgttccaa ctattctcta cttactacgg cgaagatgaa
     4561 cgattgaata tcggccccaa aggattcaca ggagaaaaat acggcggagc cacttactgg
     4621 gatacagaag cgtatgctgt ccctctttat cttgctttag ccaaacctga agtaactaag
     4681 aatctgctga aatatcggca taatcaactg ccgcaagcaa tccacaacgc tcagcaacaa
     4741 ggattaaaag gcgcgcttta tcctatggtc accttcactg gtgtcgaatg ccataacgag
     4801 tgggaaatca cattcgagga gattcaccga aatggcgcga ttgcctatgc aatctacaat
     4861 tatacgaatt acaccggcga tacggaatat ttgaaaaatg aaggactgga agttttagca
     4921 gaaatcgcac gcttctgggc agaccgtgtc catttttcaa aacgagcaaa taaatacatg
     4981 atccacggtg tgaccggccc aaacgaatat gagaacaata tcaataacaa ttggtataca
     5041 aatacgatcg cagcttgggt cttgcgttac acgcgagaaa attatttaac ttatcaaaaa
     5101 gaagctgcta tcactatcag cgaagaagaa ctagccaaat ggaaagatat cgcagagaac
     5161 atgtacttcc cagttgacga agaattagga atctttgtcc agcatgacac attcttagat
     5221 aaagatctca tgcctgtatc cgatattccc acaagcgatc ttcctctgaa tcagcattgg
     5281 tcttgggaca aaatccttag atcttgcttc atcaagcagg cagatgtact acaggggatt
     5341 tatttcttca atgatacttt tacaaaagaa gaaaagcaaa aaaactttga tttctatgaa
     5401 ccaatgactg tccatgagtc ttccctttcg ccaagtatcc attcgatttt agcagcagaa
     5461 ttaggaatgg aaagcaaagc tgtagaaatg taccagcgaa cagctcgctt ggatttggat
     5521 aattacaata acgacacaga agacggcttg cacatcactt ccatgactgg tagctggttg
     5581 tcgattgtcc aaggatttgc tcaaatgaaa actacccatg atcagttgag attcgcacca
     5641 tttctgccaa aaacttggaa agcttattct ttccatatca attatcgcgg acgtttattg
     5701 ttcatctcag tcacagatga acaagttact ttcgagttat tagaaggttc tcctctagaa
     5761 atttacgtct atgaacagtc ttattcttta gcggatcgtc tagaaataac gctaagaaag
     5821 gaggaagcaa atgtttaaag gggtattgtt tgatttagac ggtgtcatta ccgatactgc
     5881 tgaattccat tatcgtgctt ggcgaaagct tggtcaggaa atcggcatta cgatcgacca
     5941 cacattcaac gaacagctaa aaggcgtcag ccgagaagac tcactctctt tgcttttagc
     6001 ttatggcgga aaagaacact ctttttcaaa ggaagaattt gcagaattag cgaaaagaaa
     6061 aaacgattat tatttagaga tgatccagac gatcgaacca aaagatgtct tccccggggt
     6121 cgtgccttta ctggattctt taaaagaaga aaaaatcaag attgctcttg cttctgcaag
     6181 taaaaacggg ccattcctac ttgaaaaaat gggtcttaca ccttactttg atgctattgc
     6241 cgacccggca gaagcaacaa acggaaaacc tgcgccagat atcttcctcc ttgcagcaaa
     6301 agctgtcgga ctaaaagcag aagaatgtat cgggatcgaa gacgcgcaag ccggtatcca
     6361 agccatcctc tcaagtggcg ctcaaccaat tggcgtggga cgtgctgaag accttggaga
     6421 ggagatcccg cttgtccctg atacgacttt tcttacgatc gattacttga aagaaaagtg
     6481 gcatgaccat ggataatcca gagatctccg tcgtaccagt tccagagact gaaataaaga
     6541 aaatcacatt gaagaacgac tacttagaag tcgttcttct caattacggt gcccgcctgc
     6601 accaaatctt cgcaccagac aaagaaggaa aatcagaaaa tgttttattg tcttacgatc
     6661 gtttcgaaga tgttctaaca gacaaaagct tttttggagc aactgtcgga cctgtagccg
     6721 gaaggatccg ggatgcttct tggggtgagc atagattgga gaaaaactgc ggatcacacc
     6781 atatccacgg tggcacgaaa ggctggtctt tccaattttg ggaagtagaa gttttcaaaa
     6841 ctccatcatc aataggtgtg gtcttttact taaaagatga attttctacc tatccaggac
     6901 caatcactgc tacgatcact taccgtttaa caaaaaacga attagagatg acaacctctg
     6961 ctagtagcct tgttgaaacc atctgcaatc cgaccaacca tgcctacttc aacctttcag
     7021 gaaatggaac aagagacatc tatgaacatc agttaacggt ctttttagat ggtgtgcttg
     7081 aattagatca agaaaaactg cctactggaa attggacaaa aaaagaaaaa ctgccaatcg
     7141 actttagaaa atccccaaca ctgcaagaga tccttgcatg ttatccagat ggactagatg
     7201 atgtatttct tttgcatcat cctaggcttt ccaaaacttc cttacaatta ttcgagaagc
     7261 attctggtcg ccaaatgact atagcaactt ctaataaaag tatggtatta ttttctacga
     7321 ctggattcga agctgatttc tctgtcaatg gaaaacagat gcattcaaac tacggattag
     7381 caatcgaacc gcaagaaatc cctgatatcg ttcatttccc caaatttgga tcgatcaacc
     7441 tccatcctgg tcaagaacgg atcagtcaga cgatttatcg gttctctgca caataacaag
     7501 agaaaggtgt ttttatcatg gctattaccg taaaagatgt ggcgaaaaaa gccggtgttg
     7561 ccacctcgac cgtctctcgt gtcatcaacg atcatcctag tatttccgag acaacaaaga
     7621 agaaagtccg aaaaattatg gatgaactag gttatgttcc taacatgact gcccgcaatc
     7681 taggaaaacg catttccagt gccatcggcg taatcttacc ccctcttgat tcaaaagagc
     7741 ggatcggtaa cccattttat cttgagatca tggaagcaat caacgaagaa gcgcggaatt
     7801 atgatatgac tacagcaatc gctactgcta aaaattttga attgctgtta gagaacgtcc
     7861 agcgtatgca tttacaaaaa caagtagatg gttttattct agtgtattca gatagtcaag
     7921 atactgtcat tgattattta taccaaaacc acatcccttt cactttgatc ggacagcctt
     7981 atcatcatga gaacgaaatc gtttatgtag ataatgataa ccagcttcta ggtaaacaag
     8041 caacagaatt tttgatccag aatggacata agaatatcct gtttgtgaca aacacgactc
     8101 atgaaagttt gtatttcgaa cgatatttcg gttatcaaaa agcaatgatg atggctggtc
     8161 ttcctgcgtt atccagtgta gtactagagc atccggagga ttatctgtcc tttgagaaaa
     8221 cgttgaaaga aaacgaagcc acagcattgg tggtcattga cgatatcttc gcccttcgca
     8281 cgatgcagtt ggcgcaaatg tatggctatg tcattccaga gacactctcc gtcattagct
     8341 tcaacaactc gatcttctca acattaacac acccttacct tacaagtatc gatatcgata
     8401 tctccgaatt aggacgtgtc gctatgcaaa agctgcgcga attgatccat gaagaattga
     8461 gcagtggcgt acgtttggtc atccctcatc gcttgatcaa aagagagaca gtcgttcctt
     8521 tagaaaaccc atactaaata gctgactcac taaatacgct gaccatttat cctatggtca
     8581 gctttttatg taaaagaaga tactacttct aacacattgt ttatctgtaa aaaagagcgt
     8641 gggacaaaag taaagaacac ttttgtctca cgctctaaac acgtataaac ggcggaagca
     8701 gaagcaactc cttcggaaat aagctgaaat tcacaaaaat ttaaaaagca attttcttga
     8761 gtttcttctt atttcttgga gttaaacgct tctgtcccag cctctttttt ccttccttca
     8821 attttttaca cgtcgacgat tcaataaaaa accgcctaat attgtaaaga caggtgcaag
     8881 aatcgagaaa aaggcgaatg gcaagtaatg caacgcgcta actttcatcg ttcccataat
     8941 gaatgtgccg ctgactcccc acggtacgag agaattgacg gtcgcacctg aatccgctag
     9001 tgttcgtgtc aaataggttt ttgataagcc aacgtcatca aaagaacgct tgaatgtttc
     9061 tcctggaaga atgatggaca gatactgttc tccaacgatc agattgattc cgatacagct
     9121 caaggcagta aacccgacta aacgagaagg tcggtccatc ttttctttta attcttgaac
     9181 gatggtatca acgatcagat atttgatcag taacccacct aatgccaatg caagtaaaat
     9241 caaactagca gaacctaaca tactcatgat cccgcctcga gaaagcagtt catctagctt
     9301 tggatcaggt gtatttgcga cataaccatt catcaagatg tctgctgctt ttccgaccgt
     9361 caaacttttc tcatggataa aagaaagaac gagagcagct gtcgatcccg ctagtaatgt
     9421 agggatagcc ggaatttttt tgattgcccc cccaaataaa ataactagtg gaatcaatgt
     9481 ccaaggagag atccaaaagc tcgctttcat gttttggatc atttcattga tactagctag
     9541 atcggctgaa tgactgttat gtcctaatat cgtataaaaa atcaacgaaa ggacgaatgc
     9601 tgggaaatcc gtataacgca taccagcgat atgttcgaat agattaactc ctccaatacc
     9661 cgctgccaag ttggtcgtat ctgataaagg cgagcagtta ttccctaaaa aggcaccgga
     9721 aacgactgcg cctgtagtca ttgcatcatt gaatccaagt aaatgaccga tccccaaaaa
     9781 agcaattcca atcgtcgaaa tcgttgtaaa tgaactgccg acagtcactc cgacaattgc
     9841 acaaacaaga aatgctgtcg gtaaaaagaa acgcacagaa acaagtttaa atccataaac
     9901 cataatcgta gggatggttc cagataaaat ccatgaagca actaagacac caatcaaaag
     9961 aaaaatcagg attggaatga tccctggagt gattcctttg atgatcccgt catggatctc
    10021 atcccatgtg aactttttaa atctgccata aaacaacaaa cacatgaaag ctgcaaggat
    10081 cgggatatgt ggcgagagct ggaatccgat gatcagtact cctaggagtg ccaacaacac
    10141 gatcaataca gcgatacttt cttttaaaga aatattttta ttcatcgtcg atctcctatt
    10201 tcaaatgaaa aggccctgcc atgaaaaaac tattcacttg gcaggacctc ctactaaatc
    10261 agcgacaaaa cgtcggctga aagacttatt tttttatttt tcttctaaac ttttttctac
    10321 ctcaatcccc caagaatctt tcttgaagtg attgatgatc aatggaataa cgaacatgac
    10381 cactgtgata gctgctacta ataatgcatc actcaatgtc gctgtaccta atgtagcaaa
    10441 tacgacaacg atgatagaga agatcagcat gcagctgact acccaagcta atccgtttcc
    10501 gtttttacca attcggtaag gacgttcgaa ttctggatgt ttcttacgca ttttaagcaa
    10561 tgaaacagca atcaaaatgt acacgataca ataaataaca gttgtagcat ttgtaagtgt
    10621 caagaataca gcacttacgt catccattac accataaagc aaagcaaata atgaaataac
    10681 gattgattgt gttagaacga catttctaga tacgccgtat ttgttttctt tatggaatcc
    10741 gaattttggt ggaaggaatc cttcacgagc tacttggatg attgtttttg atggtccagt
    10801 aacccaagct gataattgta aaagcacacc aataaatacc atgaagctga agatatttcc
    10861 gatcacttct ggtaagccta ggatttgaca gtcaatcaag ataggctgcg tgatatttgc
    10921 taattccatt tttccatctg gtacgacatt tgctacaagc atcgcattga tgacattcaa
    10981 taaaactaat ccgatcaaag cgataaatac gccttttgta taattttttg ttgcgtcttt
    11041 caaacgaggc atgtaaacag aactcatttc gatcccaaca aaaataaatg tgataccagc
    11101 taagtatttc aatgaatcaa tatgttccaa gtcagggaaa gcttttgacc atgaaaagtc
    11161 acctagatag ccgtttgaga ctagtccgac tttgaaggct gccaaaacac ctaaaacgaa
    11221 catgatgact actgggatat atacccctag ccatacaccg atgttcccgc cgacttttgc
    11281 catatcgaat ttcaagttca agatcgtgat gatccagtag ataactaaaa tacaacctaa
    11341 aacgaaccag tgattattgc caagttctac attaccaaat gtgtttccta acaatggtcc
    11401 taaagtagaa gcgaccatta ccatacctgg gaacatttgc acccataata gccatgcaac
    11461 gacaaatccc catttgctgc ccagtgctgt ttttacccat aactgaggac cgccttcttg
    11521 aggaagcatc gtggaaagct caccggaaac cattgatatc ggaccggcaa aaaacaaaac
    11581 tgcaaaaatt gaataaaaaa tcaatgtcca accgactgcg gctaacgtag gaatactccg
    11641 aaccgttgca cacagtgcca ttgtcagacc gataaaggtc ccaagaccca ttttacttac
    11701 ttcagattta ctcaccgttg acactctcct tgattttttg gataaaataa cggtgtcttt
    11761 gaagtagttt cagcaataaa cgaaacaatt cagcgaatat ttgaggggat tctttgagtt
    11821 tattgcttga agggtttctt caaagacaac ctcaaaacaa agggatcgaa gagcttaggt
    11881 ctccccttgc tcttcagatc caaacatgcg tcagaaacag ttagtattca agctgttttc
    11941 taacatgatt accttgctta ttttgcttcg cttgccaata attggtctgc gtagattttt
    12001 tccaattttt cttgaagagc tgctttgatt ttttcagcat attcttcaaa gtgttcttct
    12061 ttgttcatgt atggtgtcat tacgctggca cgcaatacag ttacttttcc ggcacggttc
    12121 cattcttcat ctgagaatcc tagttggttc acaaattgta atgggctgtt gccatagtct
    12181 gggatagcaa agtcagtatg tgaagttaag aactcattac cgtaaatgct tcctttcacg
    12241 tatgaagaat aatcatatac gtcatggttc aatttattca ttgcaactaa gtcatcgttg
    12301 cctttttctt tgaatacata gtctaccata ttgaaatctg gataagttaa tggatgaact
    12361 tcgatttctt tgtctcctac tttaaatgac aagtcattca agaagttgta gaaacgatgt
    12421 gcaccttcga ttgatgcacc cattaattta ccatatcctg tgacattcaa aggtaagaca
    12481 tggtgtgctg cccaaacgct ggctgctgtt gcccctgctt ttgaaccttc caagatataa
    12541 gccccaagta atgctgggat atctgcgcct ttttcaaata catatgtcgc aaagtaagag
    12601 ataacgtctc tcatgcggat atctttgatg acgataccac cggcagaata tggaacatag
    12661 cccattttat gtgggtcaat cgttacagat tctgcttctt cgattgcttt atatgcactg
    12721 tgtacttctt ctaagatata atctttgttt tctgtaaaga cattgtattt gtaatgaaca
    12781 tcttttaaat cttcaaatgg aatgaagttg ttgtcttcgt ctaagaaaat cgcacggccg
    12841 tatccgccat aagcagcatc tacgtgtaag tagaagtaaa taccgtcttt ttctaatacg
    12901 cgacgcaatg caacgatttt atcgataccg tcgattgctc cttcttctgt tgatcctaca
    12961 acaccgacta cacctaggat tggtgttttt tcagctgcta gaccacgaac gattttttca
    13021 agttcgttga tgtccatgcg atagttgtgg tcaactggca ctggaatcac ttggtctaaa
    13081 ccgataccga tgatgtcggc agcttttagc catgaatagt gtttagtttg tggaaccaac
    13141 cattttccta atttttctaa atgttttccg ctacgtgctg agtgagcttt gatttcgtcg
    13201 attttttctg ggacgctgtc taacagattc atgatttctt ctgttgacaa gttcattaat
    13261 tcccaatcgc tttttccagc aactagttca ggtgtcactt ctttcattgc aagcggcaat
    13321 gatttgatgt tgcgggcata ccaaagtcct tctaagtttg ctaaagaacc atcagcaacg
    13381 atgtggcccc agccatcttt atagctcatt aattttgcaa attccattcc tacttcttct
    13441 tccatttggc ttgttgcagg agaagattca taggcaacgt tattaccatt ccatagcata
    13501 gcaaaattgt acgctagtaa agatggcatc aatgtttcag agttcatatg accccagtaa
    13561 cgtccggcat tatgccaagg aacggaatgg gtacgcatcc gcgctgaaat ttctgataga
    13621 acatcctttg ttttattcac agtgtgttca aaactagcac tgcttttttc ttctggcgta
    13681 ataataggca tgtcttgagg catgtagttt tgacgccagc ctaaatgttc atccaccaat
    13741 tcattcaaca aagctttata gatttggccg ttctcagctt tgtccccaat gaatagggca
    13801 tttaggttta gatctttcga caatgattca ctcatagtga aaccctccta aaaaaatatt
    13861 taaattttgt cgagccctac attacacctg ttcttctatt ctgtaaacta agttttggcc
    13921 tgatttttaa tcatatttta atatgtatca attatccgaa ttgttcacac ttttgaaata
    13981 attaaaaaaa acgagggaat ttacaaatgt aaactccctc gcaaagattg tattaacgac
    14041 aaccagcgtt ctattttagg atgggtttca aaattggaat gttgtaatag tatttccctt
    14101 ttttaaggta aaaacttact aaattctaat caattacttt ggccaaaaag taatttttct
    14161 taccttttcg aataaccaca aacttaccat cgaattcatc ggatggattt atgacaaaat
    14221 ttaaatcggt aacgcggtca ccgttaatac ttatagctcc gtttgaaaca tcttcacgtg
    14281 cttgacgttt agatggttca atttttgtgg aaacaagcag ttccacgata ttttcaggtg
    14341 tgcttgaaat ttcaacgttc ggcattttac cgaatccttg cgcgatttct tccgcattta
    14401 gttctttaat atttccagag aacaatgctt gcgttatttt ttgcgcttct ttcaagtctt
    14461 cttcactatg cacaaatctt gtaacttctt ctgccaaacg gcgttgtgct tcacgtttct
    14521 caggttctgt ctctactttt ttcgctaagt cttcgatttc ttcttgagac aagaaagtaa
    14581 agaatttcaa gtatttcact acatcgcgat catcttgatt caaccagaat tggtagaact
    14641 caaatggtga agttttcttc ggatcaagcc aaacggcacc gcctgcagtt tttccgaatt
    14701 tcgttccgtc agccttcagc atcaatggga tcgttaaacc gaacgcttct gcttctggtc
    14761 cttctaattt tcggattagg tctaatccag ctgtgatatt tccccattgg tctgctccac
    14821 cgatttgcaa ttgcacatcg tatgttttat gcagatgcaa aaagtcgata gattgcagga
    14881 tttggtaagt aaactctgta aatgagattc ctacttccaa acggctggca acgatatctt
    14941 ttgctaacat cgtgttgatg ttgaagtttt taccgtagtc tctcaaaaat tcaagtaaag
    15001 agatttttga caaccagtcg tagttgttca caaatgtgat atttgcatct ttaccaaaca
    15061 attttctcat ttgattggaa agtgcgtcca cgttatgttg cacttgttcc atcgtttgca
    15121 acacacgttc tgttttgcgg ccgcttgggt cgccgatcgt tcctgttccg ccgccaatca
    15181 gaatatatgg atgatggcct gccaattgga atcttttcat catcataaat ggaatcaaat
    15241 gtccgatatg catcgaatca ccagttggat caacgccgca ataaagtgaa atgctctttt
    15301 cttcaacgag ctcgcgaagt ctttcttcgt ttgtttgctg attaatggcg tcgcgccaag
    15361 ttaattcatc aataatattc attttactga cctccgtgta gtttttttat gaaagcagga
    15421 acctgctagc atatgtaatt aattatttct tcaggatagg ataggaaata aaaaaagtcc
    15481 cttggcatat aagccaaggg acgaaatctc gcggtaccac ccaatttgta tgtctagata
    15541 tccaacatac ctctcgaaca tttaacgctg tcgcgtggca atcactgcca aagactcctg
    15601 aaatgtaatt cgctttaaag catgtactgg tttgcagcga ccaccagctt tctgaaacat
    15661 tggctttaac aactacttcg tttcgatctt cgtcttgtta ttattaatat gtgtctatca
    15721 tagagcgcaa ctttttcttt gtcaacagaa aaaggatatt ttttttaaat tcctcatttt
    15781 ttcttttttg aatccgctct aattccgaac attttatttt caattattgt taaaagaaat
    15841 aactttttct gtattttttc tgcaatttat cgagaagtca ctttactttg cctcttattc
    15901 tttcttcgtt acaataagga atgggacaag taaaaaaatg aaaaagcaag ggacgaggta
    15961 tatggaaaaa aagcaaaaaa ttattttagg agccacttct gctttaacta tcgttatttt
    16021 aattctaagt ttgttttttg tcatccgatc atctacaaaa aaagaaccaa tcattcctga
    16081 aaaaaaaata acagaaaaag caaaagaacc gacagatgat aagcaacaac tagttactca
    16141 agcaaaaaat ctggctcaaa gctacgatta tgaccaagcg attgcgttat tggaaaaaga
    16201 tagtaagaac ggatctactg aaacacaaca gcttcttcaa acactaaaaa aggagaaaga
    16261 acaactgatc gactggtctg atcctactca gatctctcat gtctttttcc acagcttgat
    16321 cgtggacccg caaaaagcct tccactctca acaggcacaa ggatacaaag attatatggt
    16381 gactgttgaa gaattcaacc gttcgatcga acaactttat caaaatgatt ttgttttagt
    16441 taatttgaat gatttgatcc aaaaagacga acaaggaaat ctttccttta acgggcttgc
    16501 gctacctgaa ggaaaaaaac cattggttct ttcacaagat gatgtcagct attatgaata
    16561 tatggataat tccggttttc ctagccgatt ggtcgtatca aataacaatg aaatcaaaaa
    16621 tgtctataaa gagaaagata aagaagaaat cggtgacttt gacatggttc ctctgatcga
    16681 tgcctttgtc aagaaacatc ctgatttttc ttatcgaggc gcaaaaggta ccttggccct
    16741 gacaggttac aacggagttt taggttatcg aacatcaaaa tctcaatatg gagacaatga
    16801 aaaaacaaaa aaagagatta aagaagctaa aaaagtggcg gaacagttga agaaagacgg
    16861 ctggagcttt gcttcccata catggggtca cttgaatatg actcaagctt cactagaaga
    16921 tgtaaaaaaa gacaatgaat tgtggcagca agaagttgca cctatcctag gaaaaacaaa
    16981 tattctgatt tatccatttg gtgcagacat cagcgactgg cagccttatt cagcagaaaa
    17041 tcaaaaattt gcttacttga agagtcaagg tttcgatatt ttctgtaatg tcgatgcttc
    17101 tacaccagct tggggacaat taggtacgga tttctatcga aatgctcgga tcaatatcga
    17161 tggcattcgt tttcaatctg atttagacgg aaaaaatcct attcttgatc cattcatcaa
    17221 tgtccatgat gtctatgaca aagaagcacg cgaatctgct tgattttacg tcatttatga
    17281 accaaaaaag ccgaactgac attttatcgt cagtttcggc ttttttatgc tttatttctt
    17341 cttcggtgcg attcccttga tcaggacttc tgtccattct gctgtatatg gaagcagttt
    17401 tgcgtatatc tccggcttag aaattccttc taccattgct tgaaaataga ggatcaagaa
    17461 ctcatccgaa tatttcaaat ccacttttcc ttctttccgc cctcgatgga aaagatcaag
    17521 catgatactg aaactttctt gcgtatacgc atccatcgcg tcagaaaatt cttttttttc
    17581 tttttttgta tagtatgtca tcaaatcgat ataaaattgt ttgttcactc gattgagata
    17641 acggattttg ttttcactca tggcaatcag tgtttcttca aatggaagat tttccgcaat
    17701 caacgctttc gcttcttgcc ctaaatcatt cataaatgca aaaaatactt cataaatcag
    17761 cccttccttg ctgccaaaat atttaaaaat cgtcgcttta cttacctgag cgttctcagc
    17821 taccttatcc attgtcactc cagcggttcc tatgtctgtg ttcatcaagt caaaagctgc
    17881 ttgtaaaatc tctttctttt tctcttgtgc tctcttttca aaaccgttca tttgtagagc
    17941 ctcccgtaat tttaaacttt tataatatgt atagttcaaa tttattctga ggtttattat
    18001 aactatctat tgagaaagta tcaagctagc aattccttgt ttatgaattc att
//
LOCUS       DAIGOK010000054        17802 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.054, whole genome shotgun sequence.
ACCESSION   DAIGOK010000054 DAIGOK010000000
VERSION     DAIGOK010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.054"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(9..1310)
                     /locus_tag="NOA01_001455"
     CDS             complement(9..1310)
                     /locus_tag="NOA01_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCI0632653.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(1540..3498)
                     /locus_tag="NOA01_001456"
     CDS             complement(1540..3498)
                     /locus_tag="NOA01_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HCI0632654.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(3675..3941)
                     /locus_tag="NOA01_001457"
     CDS             complement(3675..3941)
                     /locus_tag="NOA01_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCI0632655.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(4451..6577)
                     /gene="pnp"
                     /locus_tag="NOA01_001458"
     CDS             complement(4451..6577)
                     /gene="pnp"
                     /locus_tag="NOA01_001458"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCI0632656.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(6765..7034)
                     /gene="rpsO"
                     /locus_tag="NOA01_001459"
     CDS             complement(6765..7034)
                     /gene="rpsO"
                     /locus_tag="NOA01_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCI0632657.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            7285..7848
                     /locus_tag="NOA01_001460"
     CDS             7285..7848
                     /locus_tag="NOA01_001460"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCI0632658.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(8101..8622)
                     /locus_tag="NOA01_001461"
     CDS             complement(8101..8622)
                     /locus_tag="NOA01_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HCI0632659.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(8625..9467)
                     /locus_tag="NOA01_001462"
     CDS             complement(8625..9467)
                     /locus_tag="NOA01_001462"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HCI0632660.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(9460..10185)
                     /locus_tag="NOA01_001463"
     CDS             complement(9460..10185)
                     /locus_tag="NOA01_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCI0632661.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            10361..10657
                     /locus_tag="NOA01_001464"
     CDS             10361..10657
                     /locus_tag="NOA01_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632662.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            11225..12817
                     /locus_tag="NOA01_001465"
     CDS             11225..12817
                     /locus_tag="NOA01_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCI0632663.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            12830..13570
                     /locus_tag="NOA01_001466"
     CDS             12830..13570
                     /locus_tag="NOA01_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCI0632664.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            13738..15087
                     /locus_tag="NOA01_001467"
     CDS             13738..15087
                     /locus_tag="NOA01_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCI0632665.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(15215..15805)
                     /locus_tag="NOA01_001468"
     CDS             complement(15215..15805)
                     /locus_tag="NOA01_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HCI0632666.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            15952..17304
                     /gene="brnQ"
                     /locus_tag="NOA01_001469"
     CDS             15952..17304
                     /gene="brnQ"
                     /locus_tag="NOA01_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCI0632667.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         5117 a         3603 c         3406 g         5676 t
ORIGIN      
        1 tttttctttt attctatttt tctttccgtg gactctactt tgaaataatc gatatgtgca
       61 tgctggtatt ctaataccgt gaaacggaat gatgcaatct caatgattgt tcctgctttt
      121 atttctttat atcggctaga agcaaagccc gcaagtgtag taaaattcgt ttcttcaaac
      181 tctggaatct ctacatgaaa ataacgctgg aaatcatcta atgttagttt gccggagatg
      241 atataagagc catctgactg ttccgctatc aatgcttcgt ccaaatctgt ctcatctcgg
      301 atttccccga cgatttcttc taaaatatct tcaatcgtca ctaagccgct tgtccctccg
      361 tattcatctt ttaagatagc aatatgcttt cccttttttc gcatgatcgt caataatttt
      421 tttataggga tcgtttcagt tgttacgatc ggctcttcaa tcaactgttc tactgtcagc
      481 tggtcatctt tataagattg cttgatcaaa tctggcaagg tgacataacc taaaacattg
      541 tcttttgatt ctaagataac cggatagcga gtatgtcctt gttcaatcga agcttttatc
      601 gcttctttga cggtttctga agcttcgaat acttgcatgg acgtacggtc tacttgtata
      661 tcttttgcca aggtatcatc aaattcaaac acgttttcca aataatgata ttcttctttg
      721 gtcaactctc ctttttcata agaattcaat gcgactaatg tcagctcttc ttgagacaat
      781 gtctcttctc cttcagatac gagacgcaca cctaatagct tccctagccc tgcagctgaa
      841 tgattcaata cccaaataaa aggaaacatt actttataaa aataatgaag tggtttaact
      901 acgaacaaga cgaccttctc cgtattgacg atactatagc tcttaggaag cagttcgcca
      961 ataaccacat cgatatacgt aactaaaata aatcccagcg ccacagagat tgtactgcta
     1021 atagccgaac taaaaggtag tttaccgatc actggttcta gtaaagccgc gacagatcct
     1081 tcaccgaccc acccgatgat caaaccggtt agcgtaattc ctaattgact agctgataaa
     1141 tagttatcta aatggtgtgt aacatgaaga gctagcttgg cgttctttac attttccttt
     1201 tctagttgtt ccaatcttga tcgtctgatt ttcactaaag caaattcact tgctacaaaa
     1261 aatgaagtaa ttgcaatcat caaaaataaa ataatgaaat tgataaccat attctcccgc
     1321 ctttctcttt ttctattgta ccctttttct acaaaaagat tgaaaataat gcgtattcat
     1381 atttaggaat aaaaaaggag atgcggcatt tttcagccac atctccttat cacttcaaaa
     1441 tcattgcgtt acttgttgct aaacagcttt ttcatgacct ctgataaacg gtgttcaagt
     1501 agctgacatt cgacattagt tcaggtcaaa gcgctacttt cacaaccgct ctttcaacca
     1561 tgttttccga gtatttccaa tgataagcgg aacagtttca tgttttactt cgctgtactc
     1621 tagcccaaac catgtttctg gcgaggtagc gatttttttg attgccagtt ttgcctgcat
     1681 gatttgacgt tgccattttc ctagagttgt cacattggaa actacttcat caatcaggac
     1741 aaattggaaa tcgccaactt ctcgccctgg tgtcagagag tatctttgtg gctgttttgg
     1801 caaacggcct tgtttcatca aatcatggat gacttggcga acatatacgt tgatttcctg
     1861 ttggaccctg aaacctaaga acagattcac ttggacaata aaatcagtgt ccatcatatc
     1921 tacttgatat tctttcgtat atggctcatc cgtcacttcc acatgaacaa accaatagac
     1981 tcgtgctctt ttaggccgtt tatctaaaat cgaatagatg aactgtcggc caactttatc
     2041 ttcagccata tcaggaacca agaagaccac atttgtctga tagattggta aagaagtatc
     2101 atcacgtaat tctctaagct gcgggatata atctctcaaa gacacatcct cggttgacga
     2161 ttcgcgaata atgttccctt gttcccaaat aaacatcacg actaagatga tcaaaccaat
     2221 cagaacggct acaaaaccgc ctcggaagaa tttgacaaca cttgagataa agaaaacgac
     2281 ttctatcgca ccgaagaaaa aggtaactaa atatgcaagc caacgagaat gcccattttg
     2341 gataagataa aagtaaagca gtgctgttgt catcaacatc gtcacagtga tcgatagacc
     2401 gtaagcagct tccatatgat gagacgttcg gaatgtgaca accactagag aacaggcgat
     2461 ccataaaatc agattgactg ctgggatata catttgcccg atactgttcc caggatacat
     2521 gatctttaac cgaggcagca gtttcaactt gattgcttct gaaactagcg taaacgaacc
     2581 tgtaatcaat gcttgcgaag caatgactgc agcgactgtt gcaaaagcaa cgccgatcac
     2641 gatccaactt tgaggcatca tttggaagaa tggattcagg ttcttgattt gctgtgcttc
     2701 agggttttga taaacattca acaaccacgc agcctgccca aaataattga taaccaaaca
     2761 aattttgacg taaggccagc tcagacgaat atttcgtttg ccgacatgtc caagatcgga
     2821 ataaagcgcc tctgcacctg tcgtagccag gaaaatattt ccaagaatga acaagcctaa
     2881 tttgttatct gcactaaaaa gcagctggat agcataataa ggattcagtg cacggataac
     2941 tgtccagtct tgactgaagt tcattaatcc cattacgcct aaaaaagtaa accaagcgaa
     3001 catgattggt ccgaatgctt ttccgacaat atctgtacca aagcgttgga ccatgaataa
     3061 aacaaaaata ataactaaag tgataatgac gatcacgttc tggttattac cgaaccggtc
     3121 aaaaaactgc ggaattcctc gcaagccttc aatcgcagtc gttaccgtca cagcaggggt
     3181 caatacccca tctgctagta aggcagctcc tccgatcatt gcaggaatga tcaaatattt
     3241 cccacctttt cggacaagtg tgtagagtga gaaaattcca ccttcaccat ggttatctgc
     3301 attcaatgca atcacgacat atttgattgt tgtcagtaga gtcaatgtcc aaaaaatcaa
     3361 tgagactgat ccgataacaa aatcaggatt gattcctgac aatccgccat tatcttcaac
     3421 aatcgatttc ataacgtaaa gcggacttgt cccgatgtct ccgtagacga ctcccatcgc
     3481 tacaagcagg ccagccattg atatcttttt ggtattgtgc gttgttgcca cgtttttccc
     3541 tcatttcttg ttatatggat atcctttagg taagcttgaa tgatagatga tattcttatc
     3601 catgacaccc gctttcctaa attgcttcta attatccaag taacacactg aagtgtcaac
     3661 ttatttgaat aaaattattc tttctttgga cgttctctga tacgtcttgc cacaaatgaa
     3721 taaagagcaa ggcaaaaaat tgcacccaat aatgctggta tcaaataaaa accgccgagt
     3781 tccggtccca gtggtcctag taaaaattcg ccgagccaac tacctaaaaa accgataatg
     3841 atatttccag atacgcctcc cgggacttcc tttcctaaaa ttgcgccagc tatcaaacct
     3901 aatataccgc cgataaataa cgaccataaa aaattcagca tctcctttcc ctcctttatc
     3961 catatttctc acttttattt tacgctttat tcagaaggac tccaaaaata tgcgccaaga
     4021 aactccagaa aaaaagaagt tgtaacaaaa gtgttcagct tatttccgta gaagctgctt
     4081 ctggaacacc gtgaatcaga ggttgtgaaa tcagcggttt gacctgagta ttagagaaac
     4141 aaacggaaaa atagttcctc atattttgcc gttttttgag ctaatatcga gggacagctg
     4201 attgaacatc gtttattgga gaggttgtaa aaaagctgtt ctgcatcaag taataagaac
     4261 agccaagtaa aaacagctcc taatgttttt aacttggctg gacttattac cgcagatgca
     4321 agcttttgaa caccgtttat tcgaaaaaaa aggactgtga cacggctttt gtcacagtcc
     4381 ccttttttcg aataattatt gtccccctgt caccttcacg atcacaagta cattattcct
     4441 atgaattaaa ttatttttct tcatttttcg ttgaaccatc ttcgttcaac attgcttttc
     4501 ttgacagatt tacgcgtcct tgtttatcga tttcagttac tttcacaagg atctcatcac
     4561 ctagtttaac aacatcttcg actttattga cacgatcgtt tgctaattgg gaaatatgga
     4621 caagtccatc ttttcctttg atcaagttga cgaatgcacc gaatttttcg atacggacaa
     4681 ctttccctag ataaacttca ccaactttga cttctttggt caaatcttcg atgatcttga
     4741 tagctttttt gatcatttct gcatcagctg aagcaatact tacttttcca tcttgatcga
     4801 tatcgatttt cacaccagtt tcatcgatga ttccgttgat cgtatcgccg cctttaccga
     4861 taacatcttt gattttagca ggttcgatct ggatcatttc gatttttggt gcatattggc
     4921 ttaattcttc acgtggagca gcaatcgttg aagtcaattc ttctaagatt tccatacgtg
     4981 cttgttttgc ttgagttaat gcttcagtta ggatttgttc tgtgattcct tgaatcttga
     5041 tatccatctg taaagcagtg ataccatctt ttgtaccggc aactttaaag tccatatcgc
     5101 caagatgatc ttccagccct tggatatcag ttaggatcgt atagttttca ccatcagaaa
     5161 caagtcccat cgcaataccg gcgactggcg ctttgattgg tacacctgca tccatcaatg
     5221 ctaatgtgcc agcacagata cttgcttggg aagaagaacc atttgattcc aatacttctg
     5281 ctactaaacg gatcgtataa ggaaattctt cttctgaagg gattacttgt gctaacgcac
     5341 gttcacctaa tgcgccatgt ccgatttccc gacgccctgg agagcctgca cgtcctgtag
     5401 aaccaacaga aaattgaggg aaattgtagt ggtggatgaa tcgtttgctg acttccaccc
     5461 ctaaaccatc aatgatttgg tgctctccta gaggtgcgag tgtacaagct gataaagctt
     5521 gtgtttgtcc tcgtgtgaac aaacctgatc catgtacacg tggcaataag ctgacctctg
     5581 atgacaatgg acggatttca tccaatttac gcccgtcagg acggatctta tcgatggtga
     5641 tcaattcacg tacgacatct ttttcaagat cttctgtgat ttgtttaact tctttcaaca
     5701 agtgatcttc atcttcatga cctgcaaatt tctcagcata agcttcgcgg atatcgatct
     5761 tcacctgctc gatattatct tcgcgagcaa gtttttcttc agtcattact gctgctttca
     5821 ttgtagcgta agaagcatca aagatttctt ttttcaatac ttcatcaact tgcagtaaag
     5881 tcacttccat tttttctttc cctactgctt gaacgatttc ttcttggaaa gcaactaact
     5941 ctttgatagc gtcaaaacca aacagcaatg caccaagcat gtcttcttct gaaacttctt
     6001 tcgcgccgct ttcaaccata ttgatggctt gtttcgttcc ggcaacagtc aattcgatat
     6061 ctgtttgttc tgcttgttct actgttggat tcaagacgta ctcgccattt acacgaccaa
     6121 cttctacgcc tgcgattggg ccgtcaaatg ggatatctga gattgatagt gctaaagaag
     6181 aacctagcat tgctgccata gctggcgagc agtctgtttc tacactcatc acgatgttcg
     6241 ttacttggac ttcattacgg aagccttctg caaacatcgg gcgaattgga cggtcgatca
     6301 aacgggcagt caatgttgct tccgtacttg gacggccttc tcgtttgatg aaacctccag
     6361 ggattttccc tgctgcatac attttttctt cataattgat cgttaatggg aaaaagtctg
     6421 tatcttttgc ttctttagag gccacagctg cacttaagac tactgtatct ccataacgga
     6481 ctaaaacggc tccgtttgct tgttttgcta gctgaccgac ttcgatttgt aagggacggc
     6541 cgccccatgt cgttttgaat acttgttttt ctgacataac ttctcctttt ccttctcgtg
     6601 aaagcaagac gctactaaac aaatgaaaac tgccgaaagc cgtcttttga gcagctttca
     6661 tttggttagt aggagtccat ctagcttgat cacggaattt tacttaattg atatacgagt
     6721 aaaaaaagtg agattcatgt gaatcccact ctttttgact aaaattaacg acgcaagcct
     6781 aaacgtttga tcaattcgcg gtaacgttga acgtcagttt tacgtaagta agctaacaag
     6841 ttacggcggt gaccaacttt tttcattagg ccgcggtatg aatgatggtc ttttttgtgc
     6901 acgcgtgcat gttcattcaa gtggttgatt tcttcagtta atacagcgat ttgtacttct
     6961 ggagaaccag tatccccttc atggcgagcg tattcgttga tgatttcatt cttgcgttct
     7021 tttgaaattg ccatttcttt cacctctttc gattattccc ctgcgactga gtaaaacgtt
     7081 ggtgattcgc ttaaccaagt ggcaggtcat gtgcctagag cacacagcta tactttacct
     7141 gaaaccgggt tgaaaatcaa gtagttaatc attaagaaaa ggtagttttc aaacaatctt
     7201 actactactt ttccattttt gtggtaaact ataatgcgaa aatagacttg aaacacagtc
     7261 ttttaagaaa ggggtacata ttccatgatt acaatggatg atatcattcg tgaaggaaat
     7321 ccgaccttac gcgaagttgc aaaagaagtt tctcttccat taagcgaaga agatatctct
     7381 ttaggaaaag agatgcttga gttcttaaaa aacagccaag atccaatcaa agctgaagaa
     7441 ctacacttac gaggcggtgt tggcttagct gctcctcaat tagatatttc taaaagaatc
     7501 attgctgttc acgtaccaag tcctgaccca gaggcagatg gaccttctct aagtactgtt
     7561 atgtataatc ctaaaatttt gagccattct gttcaagatg cttgtttagg ggaaggtgaa
     7621 ggctgtttat ctgttgatcg agaagtacca ggatatgttg ttcgccatgc caaaatcact
     7681 gtgtcttatt atgatatgaa tggtgaaaaa cacaagattc gtttgaagaa ttatgaatcg
     7741 atcgtcgttc aacatgaaat cgaccatatc aacggtgtga tgttctatga ccacatcaac
     7801 gatcaaaatc catttgcact aaaagaaggc gtattggtga tcgagtagag aattggcagg
     7861 ttccttcgat actatgctac aagaaagctt tataaaaaag gggagtgctc aaaagctgca
     7921 cttccctttt ttatgtttct ccactaatcg aaatttagat agacatctag aaatcgcaat
     7981 tccaaaaaat tctgtgtatt ggatacgcat cttttcttat tttttcttat ttcttccata
     8041 tgggtgttta tctcagagat gatagaatag tttctaggat aaaaagtagc agctacgatg
     8101 ttatccttcg tagaggattc ctgcttcagc tagtttttgt ttgatgtttt tcagcatatg
     8161 cttttctggg tagcggatcg tatgcagatg gatgccgcct gttaattcgg ataacaaagt
     8221 agcattactt ttttgataac gtctcataaa ggaatcgata tcttgtggcg ttttgatgtg
     8281 caaagttcct gctaactcac cgtaaagggg atgttccaca attacatcaa tgatttcacc
     8341 accattttca acgattagtc tcagttcttc ttctgtttga tcttttccat gctggacagc
     8401 gattttactg atgtgcccag tattggtttc ttctgtttcg aggatataac ctcttgctgt
     8461 tgccacgata cttttacctg cagctcgtag taaagcaata tcgcctacaa tgatctgacg
     8521 gctgactcca aaagttttcg caaacttgct agcactcact ggtttttctg tatttgccaa
     8581 ggtttgcatg atttgttttc gccgtgtctc tccttcgatc acacttattc ctccttcatt
     8641 ttgttttggt attcgcgcga gtgtttcatc aaatcaacat acgtgtctgt tattgtttcc
     8701 ttatatccct tatttttcag cagactaaca actttatcca atacttgctg ctctcttttt
     8761 gcatcataaa ctgcttggtt ttcttctttt ttcacttgtc ctattttagt taccgtatcc
     8821 attctttttt ctaataaagc aaccaactct cgatcgatca tatcgatctt atgacgtaat
     8881 gtttcaagag actcttctgt tagttcttcc tctactgtct tgatgatacg agcaggattt
     8941 cctgcaatca ctacattgtc gggaaaagat ttggtcacaa ccgaacctgc gccgactact
     9001 acgttgtccc ctagagtgac acccggcgtg acgataactc ctccgcctaa ccaaacatta
     9061 ttaccgattt taatgggttt tgcgtactcc agaccgctgt tacgtttcac tggatgtagt
     9121 ggatgcgtcg ccgtgtaaag ctggacattc ggagcgaaca tgcagttatc accgatttca
     9181 atcgtgctga catctaaaaa tgtacaattg aaatttgcat aaaaattctc cccaacaaat
     9241 atattatatc cgtaatcaaa attgataact ggctccatat agattttttt ccctgtacgg
     9301 ccaaaagtct ctttcaacaa ttgggatcgc agttccgctg attctgcttg gttgatgatt
     9361 tggctttgac ttcttgcaaa catacggtcg gcaaccagct caggatcatt ggcaaaataa
     9421 agctcgcctg caatcatttt ttctttttcg gttttgaatt cagtcataat ctgctcctaa
     9481 ttcttgtatt tttttgaaaa caaccgctcc gtcttcttga ataaacgtat aaggctccgc
     9541 taaataaaca ggcgtttgat tgattactaa gattttggct gacggctggc gaaactgtac
     9601 cagatcacaa aacggatgaa cttgaaaact cgttccaaca atgacgatca gctctgcttg
     9661 gctgacggca taagccgcct gctccatgac tccatcttga aacccttcac catataacac
     9721 gatgtctggg cgtatttgtc ctccgcatgc ttgatgtttg tcgctgtgaa gatattcttt
     9781 ccagtctacc gtttgttgac attttcgaca atgacattga tacagactcc catgaaaatt
     9841 gacgagttgc gggcttttag cctttgcgtg caaaccgtcg atgttttggg aaacaaccca
     9901 aacatttttt tctttcgcta gatgtgctat ttccaaatga atgatattgg gttttgcttc
     9961 aggatgataa atctttttga caaattgata gaatttatcc ggttcattca ccattgcttg
    10021 atgactcagc aagtattccg gctgctccat cccgtggtag attcctgaca aagatcgata
    10081 gtctggcact ccagaaggtg tggatacccc tgcaccggtc aaaaatgtaa tttgctgata
    10141 agctaatatc tcttttgctg cttcgtctac tgttaattgt ttcatgtttt gtcacctcac
    10201 tttccattat agcccatact cgaacaaatt tattgttgaa aagtcagaaa aatgggaata
    10261 aaaaagtgtt atgggcattt gagcaattta tcacaatgtt cactgacaaa agaatgaaaa
    10321 cgtgttacaa tactgctgta taaaagtgag gtgaatcgat atgttaccat tctttattta
    10381 ttggggcgtt attctggctt gtatcgcctg gttagcgtta agcgtttatt tctctgtttt
    10441 ttatcttgta cgcaaagaaa atggaaatct ttgggctttt gctttattca acgtgattgc
    10501 cgcaattgtt ttagccatta cattagccgt atatcgaaca tggggctggg gaatcaccca
    10561 atactcaagt ttgatttatc taatattagc catttatggc gtcgttgttc ttttgcaagc
    10621 gatcctagga cgtgagccaa agaaagcggc agcttaaatg atcgagatgg aactggacac
    10681 aggaaatttt ctgttctagt tcttttttta ttgtattaca tacatttttg tgtataatta
    10741 tactatcgtt tgcaatggat agatgattca cattttgatt gttttttcgg taaaagtaaa
    10801 gatttctata tttatcagta ttttttcaaa aaccagcagt tatagattga caaagcctct
    10861 cgagtatcgt aaactttttt tgtataaata actgaatacg agatgcctca atcaatgagg
    10921 tagaggtcgc gagaatcatt aaccctgtgg agtgaagcaa acatggagaa gcaggaatag
    10981 ggaggatcgc cgaaatgtgc acttttttgc ttggagtgta tgttgggacg caagttaaga
    11041 gcttgcggac tgtcttagca gtgatgctaa gttgcgctat gttttgtgag aggtttatca
    11101 ccttatggaa ttttagcggc ctttttatat ctataaatcg gtcgcttttt attttttcag
    11161 ataaagcttt accgcatttc atctagaccg ctcgtgtatc atataatcag aaagggagaa
    11221 aaaaatgaaa aaaaatatgg tttcagttgc cgtactattt gcttttgtct tctcactatt
    11281 cagtgtggga tctctggcaa atgcagatga aaaaacaccg agcgatgagt ttcgcgtagg
    11341 tatggaggct gggtatgcac ccttcaactg gtctcaacaa acagatgaga ataatgcctt
    11401 gccaattcaa ggccaaaata gttacgccgg cggttatgat gttcaaatcg ctaaaaaagt
    11461 agcagatggt ttagggaaaa aattagtcat cgtccaaacg aaatgggatg gtctggctcc
    11521 tgcccttcaa tcaggcaaga tcgacgcgat catcgccgga atgagcccta ctgctgaacg
    11581 acgaaaagaa attgatttta ccgatcctta ttacgaatca caattagtag tagtagttca
    11641 aaaagatagc aaatatgcaa aagcaaaaag tttagcagat ctttctggag caaagatcac
    11701 tgctcagtta aatacattcc actatactgt gatcgatcag ataccagatg tgcagaaaca
    11761 acaagcaatg gataatttct cagcaatgag aacagcttta gcttctggaa tgatcgatgg
    11821 ttatgtcagc gaacgtccgg aaggtgtaac ggcaactagt gtgaacaaag acttggaaat
    11881 gcttgaattc agcggtgcag atggcttcca gacaaacccg gaagatgttc agatttctgt
    11941 tggtatgcgt aaaggcgacc cggatatcca aaaagtaaac cagattcttt ctggcatctc
    12001 taacgatgaa cgaaccaaaa ttatggacca agcagtcaaa gatcagcctg ctgcaactga
    12061 tagtgaagaa aaacctggca tcatttctga tttcactacg atttggaaac aatacggcaa
    12121 tatgttctta cgaggtgctg gacttacctt gttcatcgca ttgatcggga cagttgtcgg
    12181 tactacactt ggattactga ttggtgtctt ccgttcggta cctgaatcag gaaatgcact
    12241 gactcgcttc ttccaaaaaa tcggaaatgc acttctttct atctatgtcg aagtattccg
    12301 cggaacaccg atgatggtac aagcaatggt catcttctac ggtctggcac tcgcttttgg
    12361 catttcattg aaccgaacga ttgccgcttt gtttattgtc tctctcaata ctggagctta
    12421 catgacagag atcgttcgtg gggggatttt tgcagtagat gaagggcagt tcgaagctgc
    12481 gcaagccatt ggtatgaccc atggccaaac gatgagaaaa gttgttattc cacaagtttt
    12541 acggaatatc ttgccagcaa caggaaatga gtttgttatc aatatcaaag atacagcagt
    12601 tttaagtgta atcggcgtag ctgatttgtt cttccaagga aactctgctt ccggtgcgaa
    12661 tttccaattc ttccaaacat ttacgatcgt cgggatcatc tacttagtca tgacctttac
    12721 gatcaccaga atcctgcggg cggtagaaaa gaaaatcgat ggtccttcag cttataccaa
    12781 gcttgaagaa atcgacaatt caaatttgca ggaataagga ggaagaatca tgaatgaaaa
    12841 aattattgaa atcaaacatc taagaaaaag cttcggtgaa aatgaagtat tgaaagatat
    12901 cagcgtaaca gtcaataaag gtgaagttgt gacgatcatc ggttcttccg gttctggtaa
    12961 atctactttc ttgcgctgta tcaatttact ggaaaaacca acaggcggcg agatcattta
    13021 taatggagaa aatgtattag ctcctaaata taatttacct aaatatcgaa cgaatctagg
    13081 tatggttttc caatccttca acctgttcaa caatatgaat gtcttggaaa actgtatgtc
    13141 tggtcaaaca actgtcctga aacgagataa agaaactgcc aaaaaagtag caatcgaaaa
    13201 tctggaaaaa gttggtatgg atcgttatat cgaagccaaa ccagcacaat tatctggtgg
    13261 acagaaacag cgtgtcgcaa ttgctcgggc attatcaatg aatccagatg tattgctatt
    13321 cgacgaacct acttcggcgt tagaccctga aatggttggc gaagtgctta atacgatcaa
    13381 agacttagca catactggct tgacaatgct tatcgtgacg catgagatgg aatttgcaaa
    13441 agatgtatct gaccgtgtca tttttatgga taaaggtgtg attgcagaag aaggtacacc
    13501 agaagatatc tttgtccatc cgaaagaaga acgtacaaaa gaattccttt cacgtatttt
    13561 aaatgcttag gatcaatagc tgattgggac aaagagctgc ggtgtttacc gcagttcttt
    13621 tttatgaatt actcataaaa ggtaaatttc tgaaaatttt ggaatcttag ttgtgttttt
    13681 cagaaaactc tcttaatatg gaagtaatta aactttttta gttaggatga tttaaaaatg
    13741 aatgaagagc aagaactgca gcgtggactg aaaaatagac atgtccaact gatttctatt
    13801 ggcggagcga ttggaacggg gttgttttta ggttccggaa agtctatcca gctagctggc
    13861 ccttcgatat tgctcgccta tcttattact ggatgcatct gttttttcat tatgcgtgcg
    13921 ttaggcgaac ttctgttatc aaacaccaat aaccattctt ttctggattt tgttgcagaa
    13981 tatcttggca aaaaagcggc tttcatcact ggctggacgt attggttctg ctgggtatcg
    14041 attgcaatgg ccgatttaac agccatcgga atgtatgtac gctactgggc gcctggtgtt
    14101 cctcagtggc ttcctgaact gattgcatta ggtcttcttc tttgcttgaa tatggtggct
    14161 gtctcattgt ttggtgagtt agagttttgg tttgcattga tcaaagttgt agcgatcatt
    14221 gcctttatcc tcgtaggagc ttatatgatc ctcacccact atacgacaga tattggcaca
    14281 gccagtatca cgaatctatg gtctcatggc ggattctttc caactggtgc aaaaggattt
    14341 atcttagctt tccaaatggt aacctttgct tttgctggga tagaattagt tggattagtc
    14401 gctggagaaa cagaaaatcc tgaaaaagtg ttgccggaag ctatcaataa tattcctatc
    14461 aggatcatct tattttactt aggatctctt tttgtcatta tggcaatcta tccatggaac
    14521 agcttgaatc cagataacag tccatttgta gaagtatttt cggaaattgg gatcacgatt
    14581 gctgcttcct tgatcaattt agtcgtatta agtgcagcag cttctgcctg caacagtgca
    14641 atatatagta caggacgtat gcttcgttct ttagcgcaag aaggcagtgc ccctaaaaaa
    14701 ttcaaaaagt taacaacaca tcatgtgcca gggaatgctt tgacattttc tactatcgtc
    14761 attttcattt ctgttatctt gaattacgtg atgcctagtg aagtattcac actagtaagc
    14821 agtattgcca ctacttgttt cttgtttatc tggagtatgc ttgtctacac gcacatgaaa
    14881 tatcgtaaaa gtatcctcgg caaaaaagct cattcattta aaatgccgtt gtacccattt
    14941 agtaattatc tggtatttct ttacatggcc tttgtgtgtg tcgtcttgtt cctaggaaaa
    15001 gatacgagaa tcgctttatt actaacacct gtgtggttct tgcttctatt actaatctat
    15061 catatgaaat atgaacgtaa gaagtaagaa agtagttgtg acagaagtgg tcagcttcga
    15121 gaaataagaa ggaatttacg aaaattgctt cttaaatttt tgtgaaattt cggcttattt
    15181 cttggagata aaagcttctg tcccagcctc ttttctattt ccccggaaac aaccgctgat
    15241 aataggcatc tgctttttta taggaaaaaa tccaaatacc agatacaaaa aaacgtccga
    15301 ataacccgat caacggtaca aaaaagacac cggcaatcaa gcataagtta atcatcatgg
    15361 agataagact tcgacggtcc tttgacaaaa ctctcacgac atgggaatct ggtttatttg
    15421 ctttcatgat ttgcctcatc aagataccaa aagaaaaact ccacatcgtg tatactaaga
    15481 cataagccat ctcaggccaa aaagcttcag ggttacgccc gacccaacta gtaacagaag
    15541 gacataaagt cagccaaaag agcaaaaaga tattcgacca gaccactcgg taattaattc
    15601 gttctgccat ctgaaatata cgatgatggt tcacccagat caccgcaaca aatataaagc
    15661 tgatggtata agctaataat gtatttcgga tgacccataa agaatgaaag ctagatgtat
    15721 tagggatctt gatctctaat acaataactc tcaaaacaat ggctaccact ccatcagtaa
    15781 atgcttctaa tctcgacttt gtcacgatga ttcctcctca gatgattcag cgcttttatt
    15841 ttatcactta cgttattaaa taaccatcgt tttatatagg gaattttctg aaaattgttg
    15901 catatttttc gaaattttgt tacttttaaa aaaaccgtaa aggagttatg tatgacaaaa
    15961 aaattatcat ttaaagacta tctttttata ggctccatgc ttttcggtct attttttggt
    16021 gctggaaatc ttatctttcc cgttcatttg ggtcaagaag caggcgctgc taccttttgg
    16081 gcgaatctag gtttcttggt taccggtatc ggtcttccct tcttaggggt gattgccatt
    16141 ggtgtatcga aaagctcagg tgtatatgaa ttagcaaaac ggataaatca aacatatgcg
    16201 atggttttta ccatcttgct ttacttagtt atcggaccgt tttttgcttt acctcgcctt
    16261 gcaaccacat ctttcgagat tggtttagca ccatttatcg atcctagcag acagacgcta
    16321 tatctagcaa ttttcagtat tgtatttttt gtgctggctt ggtggttttc aagaaaaccg
    16381 acaaagatat tagattatgt tggaaagttc ctgaatcctg catttttggt tttattaggt
    16441 gctttgcttc tccttgcttt tttgaatcca ctaggttctg taaatcaagc gcctattcag
    16501 ccaaattatc aagagcatgc ttttttcaca gggtttactc aaggatataa cacattagat
    16561 gcgcttgctg ctttagcatt tgggatcatc attgtcacaa caatccaaaa catgggtgta
    16621 acaaaacctg ctgaaatcgc taaagacacg atcaaatctg gtgcgatcag cattatattg
    16681 atgggaatca tttatacttt gctcgcttat ttaggtgcta tgagcttagg tagctttgct
    16741 ctaagtgaaa atggcgggat cacgctggct cagattgctg atcattattt aggtacttat
    16801 ggcagtatct tattagcatt tatcgttata ttagcttgct tgaagaccgg tattggtttg
    16861 attacagctt ttgcagaaac attcacagat ttgtttccgc aaagaaatta cgcattcttt
    16921 gttgcacttg ctagtatcct tccttgttta gcggcaaacg taggcttgac gaatatcatt
    16981 caattttcgt tgcctgtttt gatgttcatc tatccattag caatgacact gattttacta
    17041 gtgttagttg gtcctttgtt taagcagcgt acggctgttt atcgaatgac tacttatttt
    17101 acattgattg cttcgatttt tgatgggttg aacgcttgcc cagaaactat caaacaaaca
    17161 cctattgttc aaaatctttt atatgttgcg gaaagctact tgccgttttt caaattgggg
    17221 atggggtgga tcgtaccagc agttatcggt tttgttatcg gactgatttg gagtttcgct
    17281 aagaaagaag aagtagcaga ttgataaaag aggttgtgaa agagacgttc agccttgagc
    17341 aataaaaagt aattttgaaa aatggcttct cacatttttg cataaatttg acttaatacc
    17401 gagaaactga cttttgaaca ccgtttattg agaggtcgtg aaaaagccgc ttagtttcaa
    17461 gtattaagaa atattttgtg aaaatggctt ctcacatttt tgcataaatt tgacttaata
    17521 ccgagaaact ggcttttgaa caccgtttat tgagcgtcta taaactgacc tgagtattag
    17581 agaaacaaaa cggcaaagta gctccatata ctttgccgtt ttgttttgaa ctaataccga
    17641 agagaagctg atcaaacaac atttctttta taatatagat tttagacata tatatgatat
    17701 aaaaatagtt ttcgcaaaaa aacttgggag cgtcaataat tttgtgtaaa taaattgtcc
    17761 tcctgcaaaa taattagtta ctcagtaaac attgaaacta at
//
LOCUS       DAIGOK010000055        17558 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.055, whole genome shotgun sequence.
ACCESSION   DAIGOK010000055 DAIGOK010000000
VERSION     DAIGOK010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17558
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.055"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(534..1559)
                     /locus_tag="NOA01_001470"
     CDS             complement(534..1559)
                     /locus_tag="NOA01_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCI0632668.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            1834..3519
                     /locus_tag="NOA01_001471"
     CDS             1834..3519
                     /locus_tag="NOA01_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HCI0632669.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            3533..4816
                     /locus_tag="NOA01_001472"
     CDS             3533..4816
                     /locus_tag="NOA01_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCI0632670.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            4886..5755
                     /locus_tag="NOA01_001473"
     CDS             4886..5755
                     /locus_tag="NOA01_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCI0632671.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            5752..6588
                     /locus_tag="NOA01_001474"
     CDS             5752..6588
                     /locus_tag="NOA01_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCI0632672.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            6614..7681
                     /locus_tag="NOA01_001475"
     CDS             6614..7681
                     /locus_tag="NOA01_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCI0632673.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            7678..8505
                     /locus_tag="NOA01_001476"
     CDS             7678..8505
                     /locus_tag="NOA01_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCI0632674.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            8550..9575
                     /locus_tag="NOA01_001477"
     CDS             8550..9575
                     /locus_tag="NOA01_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCI0632675.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            9572..11755
                     /locus_tag="NOA01_001478"
     CDS             9572..11755
                     /locus_tag="NOA01_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HCI0632676.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            11755..12885
                     /locus_tag="NOA01_001479"
     CDS             11755..12885
                     /locus_tag="NOA01_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCI0632677.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            12882..13559
                     /gene="pgmB"
                     /locus_tag="NOA01_001480"
     CDS             12882..13559
                     /gene="pgmB"
                     /locus_tag="NOA01_001480"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCI0632678.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            13643..14170
                     /gene="msrA"
                     /locus_tag="NOA01_001481"
     CDS             13643..14170
                     /gene="msrA"
                     /locus_tag="NOA01_001481"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCI0632679.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(14371..15057)
                     /locus_tag="NOA01_001482"
     CDS             complement(14371..15057)
                     /locus_tag="NOA01_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCI0632680.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            15137..16675
                     /locus_tag="NOA01_001483"
     CDS             15137..16675
                     /locus_tag="NOA01_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCI0632681.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            16795..17262
                     /locus_tag="NOA01_001484"
     CDS             16795..17262
                     /locus_tag="NOA01_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HCI0632682.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
BASE COUNT         5692 a         3177 c         3797 g         4892 t
ORIGIN      
        1 ataatttgtc taactagaag aaaagtggga aaggatctta acaacatcgg ttcgtatcca
       61 attaaggtat cggaacgatc atcttccaag agaagtcaat caacaaaagt aaagatagat
      121 cattttttga atgcaaaata aattgatggg aagatagagc ggttattttt aatattttgc
      181 tagctattat tgaataattc ctcgaataaa acaaaaccag tagacatgtc tgttattcta
      241 tttatgtttt agagataagt atccccaaat aacatggatt gaaataaggg taaatgtgtt
      301 taaaaaaacg aactataaaa acttatacac aacgtggtta aaaatagttt tttcttccct
      361 gtggaaaaaa tagtttaaca caaacattat agttatccac agctatctaa aacagatgga
      421 aaataagaaa aattcgctaa aataccggga gggggtaacg agggatgagt gattgaaaaa
      481 agaacagagt aactgactta tcctcagtgt ttctgttctt ttataattct gttctacatt
      541 tcttgttgtt ggatcgaatc tgtgagagta atctcggttg aaagaatgat tcttttttct
      601 tcagggtatt ggtggtggat tagttggaat aaaagttctg tggctttgat tcctaactga
      661 tttgtcggaa tgtctatccg agtcaactgt ggcgaaaata gggaggacaa taagggatca
      721 ctgaatcctg cgatcgaaag atcatcaggt atctgctttc cagattgttc tgccactttg
      781 taaatactca atgattttaa tgcatcggta gaaaaaatcg cagttggtct ttctcgtagc
      841 ttcattaaat cattcattgc tgccatgcta tcttctaagc gaaagccgcc gtctataatc
      901 cattcattac ggatatctag gtggtgatca aataagcatt gacggtaacc attgagacga
      961 tcattggata cataaacttc tgtcggagca tggagacagc cgatatgacg gtgtccattc
     1021 ttgatcagtg tttccaccaa atcgtaacta tcctggaaat tatccgtgtc cactgagtag
     1081 atgttcgtgt agtcgtttcc gacttttccg attaccacaa taggaatatt gtattgatcc
     1141 aattcaaaaa gaagtgtttc atccatcgta gagcttaaaa taataattcc tcggatcatt
     1201 ttttcttgga tctttttcgc gcatttttct agctcttctt ctggtgtttt tgccgtttgt
     1261 agaatcaagt cgaagttatt ttgttcagaa acggcagaaa tggattgaat aacatctgaa
     1321 aaaaaaggat tgccataggt cgtatgagaa gtacgatgag ataatgctag aatcgcgtca
     1381 aaaccatttc ctgacagtcc tcttgcaatt ttgctcggtt cataattcaa ttctttgatt
     1441 gcttgaagta cacttgcttt tgctttatca gagatatttg gctggttatt taatactctg
     1501 gagacagttg attttgagac attagctttt ttcgcaatgt cataaatagt aggtttcaat
     1561 tttatatgca cctccttttt gggagcgttt tagaataatt ccatagtaca atagacattc
     1621 ttttttgtct atgattttga aaatttagga atagcttgca tgatcatgag agaccatatt
     1681 tttttaagaa gctattaatt attgatacat aagctttctt atttgtattt ttgtgtatat
     1741 caaaattatt taaaaaaatg ctattgacag taagcgtttt ccataatatg atcaaatcgt
     1801 tgggaacgct cccaaaaaag aaaggggaat ccgatgtata caaaactttt acaagcgatt
     1861 tatcgcgact cagatttgtc tgagaaaacc gcctctatga ttgaacaaaa agtcagtgtt
     1921 tatcagcaga agaaaggcca ggcaggcaaa ctgcattggg acgaagaaga tattttcttg
     1981 attacttatg gggatcaatt ttttgaagag cagcaaccaa ctttgcgtac atttaagaaa
     2041 ttttatcaaa agtatttggc agatacattt agagttgtac atttcttgcc atttttccca
     2101 tatacatctg atgatggttt ctccgttgta aattatcagg caatcaacga ccgtttgggc
     2161 acatgggaag atatagaaga aatgagaagc gaaacacggt taatgttcga ctttgtctgc
     2221 aatcatatgt ctgccgaaag tacttggttt caggaatatt taaacttgaa tgatacgtac
     2281 aaagattttt ttattgattt gccactggat acagatacaa gcagcgtgac tagaccaagg
     2341 accagcccgt tacttacaac gttcattgac aaaaacggga aagaaaaaca cgtatggacg
     2401 acttttagcg aagatcaagt agacttgaat tttaaaaatc cagaagtttt attgaaaatg
     2461 atcgatattc ttttgtttta cttagaaaaa ggcgcttcat ttattcgctt ggacgctgtg
     2521 ggatttttat ggaaagaact aggaacttct tgtatacacc tgccagaaac gcataaaatc
     2581 atccaattat tccgtgcaat cgctgatgaa gcagctccag gaacggtatt gatcactgag
     2641 acaaatgtgc cacacaaaga taatatcagt tattttggga atggcgagaa tgaagcacag
     2701 atggtctatc agttttcgct accacctttg acccttcaag cgattcgttc gggtaataca
     2761 agtaagttga aagaatggtt aaaaaccatc gatttcgcat cgaaaaaaac aacttttttt
     2821 aattttttag cctcccatga cggtatcgga ttgaatccgg tacgaggtat tctgccggaa
     2881 aaaacgatcc ttgaattagt ggaacagctg aagaaagagg gtgcccgagt aaactacaag
     2941 aaaaacccag atggaacgac cagtccatat gagataaacg ctacgtatct agatgcgtta
     3001 agttctccaa cagatacgac ggaatggaga agcaaacgct ttttagctgc tcattcattt
     3061 ttggtaacgt taccaggcgt gccggcaatt tatattcaaa gtgtgctggg aagtcgaaat
     3121 gattacaaag gcgtagaaaa aacaggagag aatcgaaaaa tcaatcggca aaaatttttg
     3181 ttagcagata ttgagaaaga gttgcagaca gatccattaa gaacagcagt atatggtgaa
     3241 ttgacccaga tgctatctat tcgaaaaaaa gaaaaagcct tccatccgaa tagtttgatt
     3301 tacttactgg actcagcaga ttcttttatc agttttattc gcgaagcgaa ggaagagaaa
     3361 gtcttagtta tccataattt ttccaatcaa tcagcaggtt atcttttacc aaaaggagat
     3421 tacctagacc tgttttctgg acaaattatc cattgcagtc aagaacgaaa gttgatattc
     3481 cagccgtatg aatatatgtg gctgaaaata ataaaataag aggtggaaaa aaatgaaaaa
     3541 aattattgtt actacctgtc tgctagctac tggatttctt cttactgctt gtggtgaaag
     3601 tcaaagctct aacgaatcac aacagacaac gattcaattc atgcactctt ctgtcgaaca
     3661 ggaacgatta agtgtgatca atgaactggt tgctgatttt gaaaaagaaa atccagacat
     3721 caaaatcaag caagtgccgg tagaagaatc agcgttcaat acaaaaatcg ttacgttagc
     3781 tcgttccggt aaattaccag aagtaatgga agtcagtcag gattttgcta aagtgatgga
     3841 taaagatgaa ctgattgatc agacggcagt ccaaaacgtt atcgaggaag ctggagaaga
     3901 taattattat gacggagcca aaaaccttgt tcgttcagaa gatggaaaaa gctatattgc
     3961 tgcaccaatt agcggctggg ttcaaggtat ttggtatgac aaagaaaaat tatcagaagc
     4021 tggtttttca gaaccagaaa attgggatga tattttaaaa attgctcaac atttcacaga
     4081 taaagagaat aaacaatatg gaattgctat gcctactgct gaaggaacaa tgagtgagca
     4141 agcgttctct caatttgctt tgtcaaatgg agcaaatgtt ttggatgatg aaggcgaggt
     4201 aacgatcgat acggaaaaga tgagagaagc attgtcgttt tatcaaaact tatcccaata
     4261 cacgatgcca ggctctaatg atgtgacaga aatcaaagat gctttcatga atggaacagc
     4321 accaatggca atctattcaa cttacatttt gccttctgtt tatgaagcgg ggaactcaga
     4381 aaatatcggt tttgctatcc caacacaaaa agatcaagct gtctatggca cagtatctgg
     4441 attgacgatt tcttctggtc tagacgagaa acaaaaagag gctgctgaag cattcacagc
     4501 tttcttatct caaccgaaaa acatggaaaa gtgggtcttg atgtctcctg gtggtgctca
     4561 accagtgaat aaacaagtag tcgaactgga tggttacaaa gaaaatgaag tcatcaagtc
     4621 ttttggcgag ctgccatctg agattgctag tgcttttgat gaagtacaag tctttggact
     4681 agtcggggaa aagaatttca cgaaaatggg agatatcaca agctcaggtg tgatcggaaa
     4741 agcagtgaac caagtaactg tcggaaacga agatgtcgat caagctttag ctgacgcaca
     4801 aaaaaatacg aaataacaag aagttctgcc aatggtgttc gaaaaaagta gatagctatt
     4861 ggcagattat cagggggagg tttcaatgaa tcgtccaagg aaacattctg acttaaaatt
     4921 agccatggga cttttagcac caagcatgct tttattagct gttttagtgc tgattccaat
     4981 gctttccaat attcagatca gttttttcga tatgccgctg aatcctaact cagacgccgt
     5041 ttttatcgga ttaagcaatt atcttgaaat tttaagtgat agtgaatttt atcgttcttt
     5101 aggtttaaca gttgcttaca cattcttagt tgtagcaggc agtacagctt taggattagc
     5161 cgtagctatt ttgcttaacc gcaagttcag atttaggaaa acggtgcgtt cactgatttt
     5221 attaagttat gttacgccat ctatttcttt gatttttgct tggaaatata tgttcaataa
     5281 tagttatgga atcgtcaatg atctgttagt agataggttg cagcttttca acgaagcacc
     5341 gctgtggttc gaccagccag ttgctagctt ctttatggtc gttttgtttg ctatctggag
     5401 atactatccg tatgcgttta tttcattttt ggctattttg caaacagtcg acaaaacgct
     5461 ttatgaagca gctgaaatgg acggtgcaaa tgcttggcag aagttcaaga tcgtgacact
     5521 gccggcaatc aaaccagtac tagcaactgt gattgtttta cgatctatct ggatgttcta
     5581 tatgtttaca gatgtctatc tactgacgaa taaagtcaat atcttaggcg tttatttgta
     5641 tcagacagcc tttgcgttca atgatcttgg aaaagcagca gctatatcga ttctattgtt
     5701 catcattatt gcagcggtcg tcttgctctc acgaaagaag gtggacttga aatgaaaaat
     5761 aaaaagagca ggaaaatctt attttacgcg acgattttgt tgctattagc tgtcaatttg
     5821 tttccatttt tgatcatgct gatgacatca ttcaaggatt cacaagaagc aattgcaacg
     5881 acacaaacta tttttccgaa agaatggact tttgaacatt atcaggatat tttcaatgct
     5941 gagatttttc cattcatcat gtatttcaaa aatagtttag tgatctcctt gatagcctcc
     6001 gtaactgcag tagtgattgg gatcagcggg gcatacgctt tgtcaaaact acgcttcaaa
     6061 gctcgctcga aaatcaatgc tagcttttac accgtttata tgttttccgg tattttgatc
     6121 gttgttccat tatttaaaat catttccggt ctaggtttgt atgataccaa tacagcactg
     6181 attatcacga tggtcgttca aacacttcct actgcgatat tcatgctaaa aagttatttt
     6241 gacacgattc cgactgatat tgaagaagca gcaatgatcg atggtcttag ccggatccag
     6301 atcattattc ggatcattat accattggct atttctggaa tcatttctgt cttcgtttat
     6361 tgctttatgg tttcgtggaa tgattattta tttgcttcga ttttcctgtc gacctccgaa
     6421 aaatttactt taccaatcgg tttgaacaca ttattcagta caccagacta catttgggga
     6481 cgaatgatgg ccgcttcatt ggtgaccgct ttaccagtca tcattatgta tgcgttatct
     6541 gaacgattta ttaaaggtga tttagtcgaa ggcggcgtaa aaggataaaa agaaaagaaa
     6601 gaggaaagaa aagatgaaaa aattagttgc attagaacca agaatagccg gattcaaaga
     6661 ttacgaagac cgtgagatcg aaaagaacga agtacgtatc cgagtaattt tcgctactcc
     6721 aaaaccagga acagaaattg tcgactttag aggattaagt ccatttatcg atgaagaatt
     6781 ttcagaagaa tggcgcctat tcttgccaag agaagaagga agccaacgcg gtgtggtatt
     6841 tggcgaattt caattaggaa atatgattgt cggtacgatc gaagaaacgg gcagtttggt
     6901 gacagagtat caaaaaggag agttagtctg cacttatgga cccattcgtg aaacgctgat
     6961 tacaaacggt gttgataatt ataaactacg taaacttgat cggttggatc aatggaaaaa
     7021 tgcagtatgt tacgatcctg ctcaattcgc tttaggtggt atccgggaag cacatgttcg
     7081 agcaggtgat gctgtagcaa tcttcggttt aggcgctatc gggcaaattg cgattcaatt
     7141 agctaaaaaa gcaggtgcta ctaccatcgt tgggatcgat cccatagcgc agcgtagaga
     7201 attagcagaa aaacacggtg ccgattacac gctagatcct atgaaagcag atgtcggttt
     7261 tcgtctaaag gaattgacag ataaacgtgg agtagattca gttattgaaa cgagtggcag
     7321 tgctgccgca ttacaagctt ctttaatagg gatcggctat ggcgggatca tttcttatgt
     7381 tgcctttgct aaaccttttc cagctgggtt taatcttggt cgcgaagcac atttcaataa
     7441 tgcaaaaatc gtctttgctc gagcatctag tgaaccaaac ccagattacc caagatggaa
     7501 ccgtaaacga atcgaggaca catgttggga attattgatg aacggttact tggattgtga
     7561 agaaatcatt gatccagttg tgccatttgc agaaagcgca gaagcttaca tgcgttatgt
     7621 agaccagcac ccagaagaaa gtatcaaaat gggcgttcaa gtccgaggag gagaacaatg
     7681 aaattcggta cgcaagatca agcatttttc ccaagagaca ttttagaaaa atttagattt
     7741 atcaaagact gtggatttga agaatacgaa atcgacggta agttgttaat agaaaatatc
     7801 gacacagtca aacaggcaat caaagaaaca gggatcccag tagtgacagc ttgtaacggc
     7861 tacgatgggt ggatcggtga ctttatcgaa gaacggcgtc ttaatggtgt acgtcaaatt
     7921 gctgagattt tgcgtgcact aagcgaggtt ggaggcaaag gaatcgttat cccagcagct
     7981 tggggaatgt tcacttatcg attaccgccg atggtttcac cccgaagcaa agaaggagac
     8041 tataaagcag tcagcgaatc acttcgttat ttagataaag tagccgaaga aacaggtacg
     8101 gttatttatt tggagccttt gaaccgatac caagaccata tgatcaattt gttagcggat
     8161 gcgcgtcatt acattgactc taatcaattg aaacatgtgc agatcatcgc tgatttttat
     8221 catatgaata ttgaagaaga tgatatttca aaagcattga ctacgcaaaa agagtatgtg
     8281 ggtcatatcc atttagcaga taatcatcgc taccaaccag gatctggcgc aattgatttt
     8341 actcgccatt ttcaaacgct acttcaaaat gagtatgaag gttatatggt atatgaatgt
     8401 cgtttgcgag gagaaaatga agtgaaaatt tatcaggaat cattgcaata cttgaaaaac
     8461 tgtctggaaa cagcgcgtca tttaaatgat ttagagaaag aatagagaaa gaataacaat
     8521 aagtaaataa gcaaaggaga gagaaataaa tgaagaaagt agcaatcata ggcgctggac
     8581 aagtagcaga aaaagtacat gctgcttatt atcaaaaaag atcagatgtc ttagaattgg
     8641 ccgctgtggt tgatccagtc agccagcgag cagaagactt ttgccgacgt acaggatttt
     8701 ctcatgcgta tacatcagtt acacaaatgc tgaatgagat ttctcccgat atcgtgagtg
     8761 tctgtacacc caatcgcttt cacttcgaac atgtattggc tgccctcgaa gctaatgctt
     8821 cagtgatgtg cgagaaacca ccagctatga cagctgatca agcaagaaag atggcagaga
     8881 cagccgataa aaacgggcag attttggctt atggattcca gcatcgtttt tctgacgaag
     8941 caacaatgat caagaatcat cttgatgttc tcggggatat ttattacgta gaagcgaatg
     9001 cgcttcgtcg gtctggtgtt ccaggctggg gaacgtttat cgacaaagaa cttcaaggag
     9061 gcgggccttt gatcgattat ggcattcaca tgcttgatac agcgatgtat attactggat
     9121 ttcctaaagt aaaaagcgtt cgggcgtaca gcttccaaaa aattggtacg aaaaaaaatt
     9181 cagggaaatt cgggaaatgg gatccaagca aattcacagt agaagatgca atttttggca
     9241 cactggaatt agaaaacggc aagatcatcc aactgaatac ttcgtttgct ttgaatatca
     9301 gagaagacaa actgaatgtg gctttttgtg gcgatcaggc aggtgctaat ctgtatccag
     9361 gcgaactatt cgaagatgta gatggaaagc tggtttcctt acaaaagacc acgccagtta
     9421 atgacccaca tgaactaagt atggctcatt ttgtgaatcg ggcgctcggt gaccagactc
     9481 agatgattgc agatggctgg caaggatatg tcatccagaa tctggtggaa aatttatatc
     9541 gttcggcaga aacaggagaa gttattacct tatgaaagta gaagaaaaac agtttgattt
     9601 agagtatatc aataagtatg cgagtcagat gaccattgga aatggatttt ttggtatcag
     9661 aggaagccag gaggaagact atcggaatca agtaagggga atgtttgcag ctggtgtata
     9721 taaccgacct ttaggagcag aatcagccga gttagtgaat ctcccagatg tcatcagatg
     9781 ggaaataaaa atcgacggtg aacatttttc tcttgagacg atgaaagttc ttgcatacga
     9841 gcgggcacta gatttagaaa tgggagagct taaacgctat gcgctcattg aaactgctca
     9901 aggaaaaaaa gtggaagtca ttacttgtcg tgttgctgat caaaataatc tccatcaggc
     9961 agccctgaaa gtggtcatca agccgttgac cgaagactgc caagtagatg tgaaaacggg
    10021 tatcgatgga cagcaaacca attttggtac acagcagttg gttgaaaaag aactgcgagt
    10081 atttggtgac gatttgatca gtgcatccta ccagacaaca gaaagtaaaa ttgatattgg
    10141 gattgcagct aaattcaata aagcaggtgt attccaagca aaaaatcgcc ggatcaccgc
    10201 ttccttttca gaaaagatca gaaaaaatac gtcctttacg ctggagaaga tcatcgtgct
    10261 ctctacctct cttcagcaaa cagacccgtt taagaaaagt attgcccaag cccagaaaag
    10321 cgttagttat acagaaattt tagagcagtc gatgtcttat tggacagatt tttggcatca
    10381 gcatcggata acgatccaag gcgattcttc tttcgatcag ttagcgatgg acttcgctgc
    10441 ttaccattta cagattatga ctccccgcaa tgatccaagt gttagtgtag gggcgaaggg
    10501 actaactggc gaaggttata aagggcatgt tttctgggat acggaaattt ttatcctgcc
    10561 tttcttttta tatcagtttc ctgaagtagc aaagaatttg ctgctgtacc gttatgatcg
    10621 attagaaggc gcacggcaaa aagcaagaga ttatcactat caaggtgcac ttttcccatg
    10681 ggaaagcgca cacactggtc aggaagagac acccaaattt gctgcattga atatcaaaac
    10741 cggaacaaga caagaagttg cttcagctgc cgcagaacat catatcagtg cagaccttgc
    10801 ttatgccgtt caagagtatg tccaagcaac agaggatcaa tcgtttttag aaacaaaggg
    10861 aaaaacgctc attcatgaaa cagcagcttt ttggctgagc agagctgtaa aagtcgatga
    10921 tcatttagag atacacagag tcattggtcc agatgaatac acagaatata tcgacaataa
    10981 tgcatatacg aactatctgg cgcattataa tgtggcctta gctgttaaat atcaagctgg
    11041 cgatccgctt tttttgaaag aatgtcaaaa ttttttagat aagttatatt tgccagtgcc
    11101 taacaaagaa cagctgattc cgcaagatga cacgtatctc agtaagccag ttattgattt
    11161 gaccacttac aaagaacgtc ctggcagcca gtctatttta cttgattatg cccgtagcga
    11221 agtaaatgaa ctccaagtca gcaagcaagc agatcttgtc atgctgttat acttattacc
    11281 tgagtatttt tctcaaccaa tcgtttctaa gaatttgcat tactatgaaa acaaaacaat
    11341 ccacgactca tcgttgagta aagcgattca cgccatagag gcttgccgta tccaagaaat
    11401 ggaatgggcg tatcatttat tccaagaagc gtgcctgatt gatttaggac cagatccgca
    11461 ttcatcagat gacggaattc atgctgcttc attaggagca ctatggctag cagttatatt
    11521 tggtttttca ggtattacag taacgaatgg aactctttct atttctccaa gacttccttc
    11581 agcatgggaa aaaatttctt ttccttttat ttggaaaaat cacaggttgc tatttgtttt
    11641 gacaaaaaaa caaatagtaa taaaaaaaga gacgaaagaa acattaccta tttttgtcaa
    11701 tggagaccaa taccagctgg aaaaagaact gatcttggaa cggaggggaa aataatgcgt
    11761 attctgattg cgccagattc tttcaaagaa agtctgacag ccttagaggc agcacaagcc
    11821 attcaatcag gttttcaaga agtttttgtt caggctgaat atgatctgct acctataggc
    11881 gatggaggag aaggaacggt ttcatcatta gcagacagtt taaggttgaa gcggcaaaca
    11941 gcagtcgtta ctaatgcatt tggcaaaaac aaagaaatcc agtacgcttc agacggtcaa
    12001 actgcgattt ttgaaatggc agaaatcgtc ggattgaaag atgtcccact aaaccaacaa
    12061 cagccactta ggttatccac gcgaggagta ggagaactga tacgacaatt gttgcaacaa
    12121 ggtgttcgac acatcatcat cggcgttggc ggaagtagta ccaacgatgg cggcatcggc
    12181 atggcagaag gtctaggtta tgctttttac gatcaagata atcaaaaagt agaagcactg
    12241 ggagaaaatc taggtaaaat tgctacagtt gataaaacga acgtgcctat tgagctgatt
    12301 aaagccaaaa tcactgtcat tactgatgtt actaatcttt tatgcggaaa agaaggcgct
    12361 tcttttgttt tcggaccaca aaaaggcttg aaagaaaatg aattagcagt ggctgaccaa
    12421 gccatgcatg atttttatat gagatttgcc cctcaagtaa tcgaggcatc gggcagtagt
    12481 gctggtggtg gaatgggtgc aggactgtta gcatttgctt cagggaaaat agtctctggc
    12541 atcgactacg tattggattt actggagttc gataaacggg tacagcaagc agacctcgta
    12601 atcgtaggcg aaggacgtct agatggacaa agtctaaaag gaaaagcgcc agttgggatt
    12661 gctagaagag taccagaggg cgttcctgtg atagctattt gcggaagtgt tggtaaagga
    12721 agcgaaaaag cagcagatgt tggcatttcg gccgtttttc cgatccttcc tgcagctgtg
    12781 cctttacaag aagctttaca gcaagccact gataatcttc atcgcacagc taaaaacatc
    12841 gcatctttac ttcaagtcgt tcattttcaa ggaggtaatc gatgaaaatc aaagcagcac
    12901 tattcgattt agatggcgtt cttgtagaca cagctcgtta tcattacgaa gcgtggctgg
    12961 ttttagctaa ccagctgtcc attccattca ctgagaaaga aaacgagcaa ctgaaaggaa
    13021 tatccagaac cgagtcacta gagcgattgc tgtcatttgg aaaaatggaa cagaaattct
    13081 cagaaaaaga gaaatcagcc tttgctgagc aaaaaaacaa tctatatctt caagcgattc
    13141 aaaaaatgga tgaaacatcc gttttgcctg gtgcaatagc tgttcttgag tacttgaaaa
    13201 aaacaaacat caagatcgga ttgggatcag caagcaaaaa tgcacgcctc attttagaaa
    13261 aaacgaattt gacaagttat tttgatgtcc ttattgacgg cacgcaagta tcaaaagcaa
    13321 aacctgatcc agaagttttt ctcaaaggag cacagcaatt gaatgtaccg ccaaatgcat
    13381 gcttggtcat tgaagattca gaagctggtt gtcaagcagc tttggctggt aacatgcatg
    13441 tattgggaat aggtgaaaac ataaatcttc cttctgctga atatgtgatt cctgatttaa
    13501 ccgtttttga tcaagtacgt tcgttttggc atttatcaga agccgtatca cgtatataat
    13561 agtttccgtt ttttctgtca caagcaaaac gttatcctcc ttttggaaat caaggtatga
    13621 tgagaaaaaa gggggatttt atatgacaaa aaaagcagtt ttcgcaggcg gatgcttttg
    13681 gtgtatggtc aaaccatttg aagagcagcc agggatcatt tctgtgacat ccggttatac
    13741 tggcgggcat gtaccaaatc caacttatga acaagtaaca acaggcacaa cagggcatac
    13801 agaagcagta gagatcgaat atgatccaga agttatttct tatgatcagt tggtggaaat
    13861 ctattggcag caaaccgatc cgactgacgc attaggacag ttcgctgatc gcggtgattc
    13921 ttatcgtcca gtgatctact ataatgatga agaagaacga aatattgcag agcgttcaaa
    13981 acaagcattg caagacagcg ggcgattcga taagccaatc gtgacaaaaa tcgaaccaag
    14041 aagcgaattt tatcctgcag aagattacca tcaggattat tacaagaaaa acaagctcca
    14101 ttacaatctt tatcgagaag gttctggacg tgcaggtttc cttcgtcgac attggggcgt
    14161 caagaaataa gaagccgaaa aagctactta tggaacaccg tatatcagag gttgtaaaac
    14221 cagcattttg agctaatact gagggtcagc tgattgaaca ccatttagtg gagaggttgt
    14281 gaacctgtcg cctagctcca agtcacaagg atttggtaag agaacactat atatcagagg
    14341 ttgtgacaag tcgtttgtca caaccttttt tcaataccta agatgaagga caatcagcaa
    14401 cgcactgctc aaaaaaggaa tgaaaggcag cttttccaac ttttttttat gacagataaa
    14461 gtaaacagaa aaaacggata ttccaaacag actagcaaga aacaagatct gaagcaactc
    14521 ttctagagac aagccaccac tccaagaaag catcaacaga gtatcgccag ctccgagata
    14581 ggattgacca tagcgaaggc acacaccgcc tacgataaac gttaacagca cactttgcca
    14641 ttggaagacg ccggcctgca accaaaaaat ccataaaatt gcccaagaac agaatagcgt
    14701 gtgagaatgg accatgaagt aaaataaatc catcaacgaa aatagcaaag ccgaaagcat
    14761 ccatatgaac gtcgaaaaat ttcttgcgga agaaaaagta aagaaccagg caaaaagacc
    14821 accacataag gcttctgcca acaaatagta gaagggcaac cgacacctgc aatagcggca
    14881 gcgaaatcgt tggacgatga tcgagagaat cggaatcaat tcataccaag aaagcgaacg
    14941 acagcatttg acacaatgag atctaggata aataatagag tgacctaacg gaatccgctg
    15001 ggcggttaaa cataaaaatg atccgataca gcaaccaatg ataaaataaa gtaacacgcc
    15061 atctccccct tttcaaaacc aagatacgca agcttaaact aaatttttgt acatacataa
    15121 aatttgaggt gaaaaaatga acgtttcaat cggattagat atcggaacca cacaaaccaa
    15181 agcagttgct ttcacagatg aagcccaaga agcagcatcc gcttattttc gctacccttt
    15241 gatccaagaa acacaaggga tggcagaaca agatcaggaa ttgatctttc aaggtgtctg
    15301 caccgttata aaagaagtaa ttaaacagtt gccttcagac acaatcattc aatttgtctc
    15361 cttctcctct gcgatgcatg gcctgatatt gttggatgaa gagaaaacac cgttatctcg
    15421 aatgatcact tgggcagata accgagcagc ggacgaagca gaaaaactga aatataccgt
    15481 gcttggcaaa gaattttatc ggcagactgg gacgccgatt catccaatga caccattgaa
    15541 caaaatcaag tggctgcaaa acagccaacc tgaattattc aaaaaaactg cttattttgt
    15601 cggtatcaaa gactatatct tttatagatt gtttggcgaa ctgatcagcg actacggaac
    15661 agcttcagga actggatatt ttgatatcca tgaattccaa tgggcagaag aagtgcttga
    15721 ctacttgaac attcgaatag agcaattgcc gcaagtcttt ccttccacct atcaagtaac
    15781 aggtctacca tcaaaaacag cagcagaact aggtttgcca aatgatatat catttgtttt
    15841 gggaggggca gacgggccgc tttctaatct agggttaggt gcttttggtg atacgatcgc
    15901 cgctttgacc gtaggaacga gcggagcact tcgttttatt actactcagc ctcagttcca
    15961 tccagaaatg gagacgtttt gttatgttct ggatcagaaa cattgggtag tgggaggagc
    16021 gactagcaat ggtgctggca tctttgattg ggcagggcat acatttttac aggaagtcgt
    16081 tcaatctgct aaagaaaaag gagaaaatcc atatgatgca ctgctttcgg ctatcgcttt
    16141 tgtgccagcg ggtgccaatg gtctgatttt tcatccctat ttacttggtg agcgagcacc
    16201 gctttgggac gcagatgcgt ttggcagttt tatcggttta cagcggcaac atacagaaaa
    16261 agaaatgatg aaggcgattc tagaagggat ctgtctcaac ctttatcgga ttttaaaagg
    16321 agtttgccag gaaaatcagc tcacagagat ccgtgctaca ggcgggtttg ctcaatctcc
    16381 gatttttcgt cagatgatgg ctgatgtact gggttacccg cttgttttcc ctactgtcac
    16441 agaagcatcc gcattaggag ctgtgatttt aggctggaga agcatcggaa agatccaaag
    16501 tatcagtgaa gcacaggaat tgatagaatt gaaagagcaa gttggagtac aagaaactga
    16561 tcataaaaaa tatcagaaaa tctatccatt atttgtttcg acacaaaagc atttagcaca
    16621 gacttatcag caatttgctc aactgcgaga agaattagct gattctgaaa aatgattggc
    16681 tttacggata aaaaagcgaa ctgaaaatcg ttctcaggta aatgattttg ttgtactttt
    16741 cattgaaagt ctactattat tttaagtgta agaaaatgtt aggaggcaag aaagatgaaa
    16801 ttccaacaaa ccaaagaagt attaaatcaa ttagttgcag atctaagcca aatgtctgtt
    16861 gttgttcacc aaacacattg gtacatgaga ggaccaggtt tccttacatt gcacccaatg
    16921 atggatgagt tcatggatga tttgaacgct cagttagatg aagtatccga acgcttgatc
    16981 acattagacg gttcacctta ttctacattg cgtgaatttg cagataacac aaaaatccct
    17041 gatgaaattg gccgctggga tcgtactatt ccagaacgtt tggaaacact tgttgcaggc
    17101 tatcgttatc tagcagatct ttaccaaaaa ggaatcgaag tttctggaga agaaggcgac
    17161 gatccaacac aagatatctt tatcgaattc aaaacagcca cagaaaaacg tatctggatg
    17221 atccaagctc atttgggcaa agcgccagag atcgacgcgt aaaaactgtt gttgaacttg
    17281 accgaaaaag aggttgtgaa atcagcgttt tgacctgagt attagagaaa caaacgtcaa
    17341 aatagttcct catattttgc cgttttttga gctaataccg agggtcagct gattgaacac
    17401 cgtttaccaa gaggttgtaa gcctgccgtt cagctccgag tcacaagaaa caattttaaa
    17461 aaacagcttc ttatgtttta tggatattgt gacttgtgac cgaggagttg gcaggagaac
    17521 accgtttatt caaaataagg ggttgtgaca agactttt
//
LOCUS       DAIGOK010000056        17481 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.056, whole genome shotgun sequence.
ACCESSION   DAIGOK010000056 DAIGOK010000000
VERSION     DAIGOK010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.056"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            79..561
                     /locus_tag="NOA01_001485"
     CDS             79..561
                     /locus_tag="NOA01_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632683.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            758..1648
                     /locus_tag="NOA01_001486"
     CDS             758..1648
                     /locus_tag="NOA01_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HCI0632684.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            complement(1935..3209)
                     /locus_tag="NOA01_001487"
     CDS             complement(1935..3209)
                     /locus_tag="NOA01_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCI0632685.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMNSVSGSMSFICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHCYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            3482..5953
                     /locus_tag="NOA01_001488"
     CDS             3482..5953
                     /locus_tag="NOA01_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HCI0632686.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(6056..7792)
                     /locus_tag="NOA01_001489"
     CDS             complement(6056..7792)
                     /locus_tag="NOA01_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HCI0632687.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSVFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7789..8493)
                     /locus_tag="NOA01_001490"
     CDS             complement(7789..8493)
                     /locus_tag="NOA01_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCI0632688.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8624..9127
                     /locus_tag="NOA01_001491"
     CDS             8624..9127
                     /locus_tag="NOA01_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HCI0632689.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            9087..9425
                     /locus_tag="NOA01_001492"
     CDS             9087..9425
                     /locus_tag="NOA01_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HCI0632690.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            9446..10864
                     /gene="ffh"
                     /locus_tag="NOA01_001493"
     CDS             9446..10864
                     /gene="ffh"
                     /locus_tag="NOA01_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCI0632691.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10976..11554
                     /locus_tag="NOA01_001494"
     CDS             10976..11554
                     /locus_tag="NOA01_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCI0632692.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            11506..12078
                     /locus_tag="NOA01_001495"
     CDS             11506..12078
                     /locus_tag="NOA01_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCI0632693.1"
                     /translation="MFPQRRGRTYRQKGRIKHAKIIFYGAISLDGYLATKTDDLQWLF
                     DTPTGKQTTYEAFYETIDTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPD
                     AEVVNEDPVAFLKNLQMTEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIP
                     LFQKGTYEARFEMLDTTRFGEFIELHYVRK"
     gene            complement(12157..12711)
                     /locus_tag="NOA01_001496"
     CDS             complement(12157..12711)
                     /locus_tag="NOA01_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCI0632694.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12964..13239
                     /gene="rpsP"
                     /locus_tag="NOA01_001497"
     CDS             12964..13239
                     /gene="rpsP"
                     /locus_tag="NOA01_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCI0632695.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            13251..13502
                     /locus_tag="NOA01_001498"
     CDS             13251..13502
                     /locus_tag="NOA01_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HCI0632696.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            13627..14418
                     /locus_tag="NOA01_001499"
     CDS             13627..14418
                     /locus_tag="NOA01_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632697.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            14588..15109
                     /gene="rimM"
                     /locus_tag="NOA01_001500"
     CDS             14588..15109
                     /gene="rimM"
                     /locus_tag="NOA01_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCI0632698.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            15099..15860
                     /gene="trmD"
                     /locus_tag="NOA01_001501"
     CDS             15099..15860
                     /gene="trmD"
                     /locus_tag="NOA01_001501"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCI0632699.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15996..16343
                     /gene="rplS"
                     /locus_tag="NOA01_001502"
     CDS             15996..16343
                     /gene="rplS"
                     /locus_tag="NOA01_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCI0632700.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            16601..16894
                     /locus_tag="NOA01_001503"
     CDS             16601..16894
                     /locus_tag="NOA01_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HCI0632701.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
BASE COUNT         5768 a         3146 c         3581 g         4986 t
ORIGIN      
        1 aaaagttttt ccccctaatt tttttagata gaaaattgta tagtatgatg agagaaaaag
       61 tagtttgggg gaacgttaat gagaagaaaa gcagtaatcg atcggcttta tttatttttt
      121 tggttttttc ctatcgtttt gctacctttt acatgggtaa atgctgttgt acaaggtgcg
      181 gagatattag gcctgatcca aggaagcgga tttgtggttt ctaggaattt tccagctttg
      241 attgttttct atttagtgat aagtgtaggt ctcttattac caaaatctct gatcaatcac
      301 tacaattggc agctgcttta cttttcttgc ttattagtat ttacatctat gttttctttg
      361 ctgcctcgct tgattatcgg ccctaatggt ttgagcgaga gtggattgaa ggatttagct
      421 tattcctcca tgtatttctt gacgatcaaa ccatggttct atttagtaat tgcctcattt
      481 attcttgcaa cattttttta cttctggaca gaaatcagga taaaaagtga acgatctgta
      541 tctataaaaa ataactattg atggtggatc atcagtaaaa aaacatatga gctgcgtaaa
      601 atgcatgttt ctcatatgct ttttttatta ttatgtaaag aatgcgaaaa acgtttacat
      661 gatttttaca taacttaact atatttttac agcaaccata cgagggattt acagtcgact
      721 gataaacttt agttgtaaat aagaaaggag caataaaatg aaaaaagtat tgtttactct
      781 aggtatgatg ggaattcttt tagccggatg cggctcagga tcaggaaata cagatggctc
      841 tgccacacaa aataattcaa gtgactcaaa tgaacaagta aaaatcgttg cggtaggttc
      901 gacagcatta cagccgttag tcgatgcagc acaagaaagc tttgtacaag aaaatccaaa
      961 ttatcaaatc tctgtacaag gaggaggaag cggaactggt ttgagccaag tcgaagctgg
     1021 agcagttact atcggaaact ctgatgtatt tgcagaagaa agagacggag ttgatgcttc
     1081 aaaattagtg gatcataaag tagcagtggt cgggatggca ccaatcgtca ataaagatac
     1141 agatgtcaaa gatatcacca aacaggaatt gattgatatc tttacaggaa aaatcactaa
     1201 ctggaaggaa gttggcggga aagatcaaaa aatcaacgtc gtgaaccgtg caaacggcag
     1261 cggtacccgc gcgacattcg aaaaatgggg actggatggt gctacccctg tgcagtccca
     1321 agaacaagat tcatcaggaa cagttcggca gctggtaagt caaacaccag gagcaatcag
     1381 ctatttagct ttctcatatc tcgatgattc cacacaagca ctaagtatcg acggtgtaga
     1441 accaaaagaa gagaatgtag cagataacag ctggggaatc tggtcatatg aacatatgta
     1501 cacgaacgga aaaccttccc ctgaagtcca aaaattctta gactacatga tgacagaaga
     1561 aatccaagaa ggacctgtca aagaattggg ctatttacca atcacaatga tggaagtaga
     1621 acgtgatcac gaaggaaata tcaaataact agcagataaa catgccacgg tagtcgttac
     1681 tattaaggaa aaatcaacta tgtttcccca catatacatt tttcctttaa ttgtgaaaaa
     1741 tagttcctca tattttgccg ttttttgagc taataccgag ggtcagctga ttgaacaccg
     1801 tttattcgaa aaaaagagct acgacataga actgtcgtaa ctttttttgg ctcttcgtca
     1861 aatcgtgttg gtgaagacaa atttgaataa aacttagtcc gatatgcggc gcgttctgtg
     1921 cgccgcatat ttttttagtt ttcaaagatc agaccttgtt gtaaatagat ccgtgcccga
     1981 aagttataaa agttgcgata accaaacgca attcgtttaa tcagcttgat cttgttattc
     2041 agcccctcag tgacgccgtt tgagtgggac accttaaagg catttgaaat acccgattgg
     2101 tagcaatgga agatcgtgaa tttcttttta aacgactgag gtaacgtaga tgaaattgtt
     2161 ttaagggttt cttgaaagcc ttccgtacct cgtttaacga aataatagcg taataattga
     2221 caaaaatgat acgcagttcg caattcttcg ttgtagctta acagctcatc aacgatttct
     2281 gtttgactaa ttggtcgttt aaacagtcga tgataatgcc gtttgagtgg ctcccatgtc
     2341 gtggaatctt tgagtaagag tttccaatat cgcttgagcc gacggtactt cttctgattt
     2401 tcagagtgtg aggttcgaaa acgattcatg atctggacac gtaattgatt gaagctgcga
     2461 ttgatgtgtt ggacaatatg aaagcggtca gttacgatct ccgcatttgg aaagactgtt
     2521 ttaagcaact gacaatagct ggcattcatg tccatcacta ggtacttgac ctttaaacgg
     2581 gcctttctag gaaagcgcag gaagtattga gttagtttgt aaagtctgcg atcttcaagg
     2641 atcgtaaata gctgattggt tgtcccatca acacaaataa aactcatgga accagaaaca
     2701 gaattcattg atttgaactc atccatacac aacaccgtcg gaaggaagtt catacgtggc
     2761 tggtaatgct tcgttgcttc acgcaagatc cgtgtcaccg tcccatccga gacgaagtac
     2821 ttcttagcga ttagtttacg tgattgattt tcgtacagtt ccatgagaat cgcctgcttc
     2881 agttcctttg ttagttgatg atgttcatca actaagtccg ttttcgcaga aaaggtcctt
     2941 ccacaatcat ggcagagaaa acgggtacgt tttaattcta aaatagttaa cttttctttg
     3001 actttcaaca tctgagtgcg tgtcgtatag gaaccgttgc ggataatacg aaccgatcga
     3061 cagtgttcac aataggaaga agagtcatcc aattttcctt taataaactg cgcttgaacg
     3121 cctttatatt ctttttcaat cagccattct tcatcaaaaa ataaatgttt atctgttaat
     3181 ccgagtaatt ttctagtatg attatccatt gagagactcc tttggtagga tgacgttttg
     3241 gtcgactaaa ttttaccaaa agtgagtctc tttttgagtt gaaacgaaga aaaagtgtta
     3301 gtagaaaatc gccttcgatt ttccaccaac acaaaatatt atagaaccct ttttttttgc
     3361 tatatgacag gaatgaaagg tgtttgcaag cttaatgtga ggaacacttt ccatatctgt
     3421 tctatactgg ataggagaag tttggaaata tccagaaaac gaaatcaatc gaggtagcca
     3481 catgaagaat aaatggataa atcgaagtaa aaaaaagaaa gtaacacaaa gatcatcaga
     3541 taaagtcatc cgtctttttc catcgtataa agaaggatta acggcagatc aagtcaagga
     3601 acgaatagaa aaaggtgcag caaataattc agtcgatccc acattcaaaa caaatcagca
     3661 aatcgtattg gaaaacatat ttacgtattt caatttgatc tttcttatct tagccatttt
     3721 gctttgttta gtagaatcct ataagaactt gactttttta ccagttatca tagccaatac
     3781 aggaatcgcc atttttcagg aaattcgatc caaaaagatc ttggacgaac taaatgtttt
     3841 aaatacagca aaagtcaatg tcatacgaga cgggatagaa caaacaatag atatagaaga
     3901 ccttgtaata gatgacattg ttattttaga gactggatat caaattccag cagatgcaga
     3961 ggtcgtggaa gggaaactgc aagttaatga ggctttattg acaggcgaag cagatgaaat
     4021 aaccaaagaa ataggtgatc cattaatgtc cggcagtttc attgtttccg gaaaagctta
     4081 cgcccgatta gacaaagttg gagcagattc gtatatttcc cagctgacag tcaaagccaa
     4141 gacaatggga gaaggagaac agtcggagat gattgcttct cttaatcaat tgatcaaatg
     4201 gattgggatc atcattattc ctatcggtat cctgcttttt tctcaaagtt acttttttaa
     4261 tggaaacacg atcaaagaaa gtgtcgttgc aatggaagca gcattgatcg gtatgatacc
     4321 tgaaggactg tacttgctga cgacgattgc attagcatta agtgcgacta aactagcaaa
     4381 gcaaagagtt cttttacaca atatgaaaag cattgagaca ttggcaaggg taaatgtcct
     4441 ttgtgtagat aaaacaggaa cgatcacaga aaataaaatg tctgttcaaa aaatgatcgt
     4501 ctctaaaaaa caagaaataa tcagtcagtc agatcaattg gaagcatgca ttgctgacta
     4561 tgcaaaagcg atggcaggag acaatgcaac aatggaagct gttaaagcat attttacaaa
     4621 aaccactgat caatcctatc accatgttat tcctttttct tctgtccaaa aattcagcag
     4681 tgtagcgttg gcagacaagg tgtatgtttt aggagcgccc gaaatggttt tgcgcgatca
     4741 attgccggaa tatgcggaag agtttgtttc tttcgctgaa aaaggctatc gcgtgttggt
     4801 ctttggtgtg tacgatggga ttcttgaaga accagtattg acagaagcag tcacaccatt
     4861 aggctatatt ctgattgcta atcctatcag aaaagaagca agagccacat ttgattactt
     4921 caaaaaacag caagtagcca tcaaagtgat ttctggagat aatccgctca ccgtatccaa
     4981 tgtagctgtc caagcaggga tttcacatgc tgatcagtac gttgatgcat ctaaattatc
     5041 agaagaagaa tatgaagcag ctgtcgaaaa atatacagtt tttggacgcg tcaagccaga
     5101 acaaaagaaa atcttcgttc aattgataaa gcgtaacaat acagtagcta tgacaggtga
     5161 tggcgtgaat gatattctag ccatgaaaga agcagactgc agtatagcga tggcttccgg
     5221 aaatgaagcc gccatgcagg cctcacaagt ggtcttactt gattctgact tttcacgcat
     5281 gccagaagtt gttgcagaag gacggcgcgt tgtgaacaat attgagcgat cagccagctt
     5341 gtttttagtg aagaatattt tctccttttt gctctcactg ttttcagtga tctttgctct
     5401 gacctatcct ttagaaccat cacaaatcac attgattagt ttgtttacta tcggacttcc
     5461 atctttcttg cttgctctag aagaaaacaa aaagcgaatc agagggaaat tcatcatgaa
     5521 tgtgatggaa aaagctgtgc caggtggact aacagatatg atcgtagtcg gtgcgttggt
     5581 gatctgcggg gtgactttga atctaaataa aacagatgtt tctacagcat ctaccatgct
     5641 tttgattgca gtagggttcc tcgttctcta taaaatctgt tcaccgctga ataaattcag
     5701 aagtcagatc attcttttct gtgctagcgg taactttttt tcggtcatct ttttgcataa
     5761 gctgttttca atcaccggta tctcagctgt atcacttttg ctgttgagca ttctattttt
     5821 ctcggcagat tcgattttta ggcatttgac cacattggta gaatatttgt ttgaacgtaa
     5881 aaatgaaaaa gagcaaaagc cgtttagtcg cgtagctaaa aaatttctat cgatatttaa
     5941 acgagaaaat tgacgaaaag tatctcaaaa aagagtaaaa aagaacaacc actcgtgtat
     6001 ctttcgaagc aggatgagtc atccaacttt aaaacgcacg agcagttgct cttttttaca
     6061 ttttcggaat ggtaacggtg aaagtcgaac ctgttccaag atgactgtct acagtaatcg
     6121 taccattaag gagttgcacg tagtctttta caatcgctaa ccctaatccg gtcccgcctg
     6181 aatgtcggct tcgcgccttg tctacgcggt aaaaacgctc aaagatccgt tcctgatcct
     6241 caatgtcgat acctatacca aagtcttgga cagaaaacga tagatcatct gttgcctgat
     6301 agcggataat aatctcgctg tcgctttttg aatattggac agcattttca atcagatttt
     6361 tgataattgg ataaaggata tctgttttag tagtaaatgg ttcgttcttt ccaatcaatc
     6421 gaatcgtcaa ccgtttcttt tcgatgattg gctgataatt ttgaacgatt tgctggaaat
     6481 aaggttctgt ataaattgtc tggatctcgt aagaattctc gccatctttt gacagctgga
     6541 tgatttcccg aatcagttta tctaaccgga tcgcatcttt ttgcatgatc tcaagaaaag
     6601 aagtcagagt ttgcgggtca tcttttgcac catccagtaa agtctcggta aaaccaatca
     6661 gtgaagtcac aggagttttt aattcgtgag agacgttgcc aacgaaatcc ttttgtagct
     6721 tttctaaacg tcgcactttc gttaagtcat acgccacccc tagaatctgt ccggtacctt
     6781 ctgaattagt aaagtagcgc aaactcaagt ctaatgtctg ccgaccttct gtaagggtga
     6841 tctcctgatg gatcagcgga tgctcaggag tcacttgatg aatcagctga atgagttttg
     6901 gctcttgaat gacttctgtg taccgctgtt caggttgata gcttaccacg tttaaatgta
     6961 gctgcatctt tggattaagc agtagtaaac gagaatcagc atcatcaatc aggaatacgc
     7021 caatcatcag ttcgttcagt aacgtatata attgctcttc tgtggtagta cacgcccgat
     7081 acatcttact catttgttcg ctcaacgcat taatcgtctg atacaattct tcccattgat
     7141 cgggtgtttg catgattact tccgcctgat taggattttg caccattttt tttagtacgg
     7201 gcagcacggt tttcaatggc tggttttttt gatagatcag ataatagatc attcctgcaa
     7261 gaacaaggaa gaaagacaga aagaagaaaa agacccaacg gcgaaaactc tcagttctcg
     7321 gtaaaaaaac gcttgtcggt tctgcgatac gtaaaatggc ttctaactgg ccactttttt
     7381 tgactggtaa tgcgacatat aaaagatcct cattcaaagt agtacttttg cgcaaagcgc
     7441 tacctaggtt cccaccattc aatacggctt tgatttcggg acgggagttt cgctgttcat
     7501 tcaatgtttc attgtttgtg tcaaataaga tctttccagt agcatccatt aaagtaatcc
     7561 gttcgttcga ctgatggacg aattcttcca aagcagtttt gttttgcgct gcttggaggt
     7621 tttcgacatc taattgccgg atcagcagtt gtgctttttt tgtcaggtag ctttcttgct
     7681 gttccacgat ctgctgttga aaataatggg aaatcaattg ccagccacca aaaaacaaac
     7741 taactaaaag aataagaaag aagagacgtt cataaatttt tttccatttc atcgtttggg
     7801 ttcctgaaac ttatagccaa atcctctaat agtcaccaag tactttgggt gttttggatc
     7861 tcgctcgatt ttttctcgca aatgactaac gtgtacatct acgattcggc tttgtcctgc
     7921 gaagtcaaaa ttccaaatgc ggtctagcaa tgtgtctcgg tcgatcacac ggtcttttcg
     7981 tttcatgaaa taaacaagta attcaaattc tttcggtgtt agttcaatca gatggtcgtc
     8041 tacggtcact tgataattgg tcaaatcagc agtgatttgt ccaatcgata gatattcagg
     8101 ctcagcttca tcttttgatt gcttacgagg ttcaattcgt ctgaatattg ctttcatacg
     8161 ggctaatact tcgcgaggac taaatggttt ggtcaaatag tcgtcagcac ctatttctag
     8221 gccaatgatc cgatccactt ggtcatcttt agccgtcagc atcaaaatcg gtgtttcatt
     8281 tttttcttga cgcagccgct gagtgatctc catgccatcc atcgttggaa gcatcacatc
     8341 taaaatgatg aaatcaaatg attgttctaa tgctaattct aatccttcgc ctccatctgt
     8401 ggcactggtt accttgtagc cttctttttc caaattgaac gtcaagagcg ttacaataga
     8461 aggttcatca tcaacaacta gtattttttt cattcaaatc tcctttaaat aggtttcatt
     8521 ctgtttatat tttagcatag aatcagtaca aagggctatt ttacaaaaga aataaatggt
     8581 ataataggaa ggcactttgc tgaatagaaa ggaagagcag tcaatgattg gaatcagtgc
     8641 atgtttaggc ggtctggcct gtcgctatga tggacagaaa aaagaagtac cagaattgat
     8701 ggaactagtt gaaactggag aagctgtcat ggtctgtcca gaagtgatcg gcggattacc
     8761 aattcctaga gatcccgctg aaatcattgg tggaaatgga ttcgatgtgt ggaaagatca
     8821 agcaaaagta tggacgacaa gcggcgaaga tgtaacggaa gcgtacaaaa atggcgcgat
     8881 caaagcctat caaaaattgc aggaaaaaca cattgatcta gtcatcatga aggaaaaaag
     8941 cccttcatgc ggaacccatt cgatttatga cggaagtttt tccggaataa aaaaaaatgg
     9001 tgccggagtg gctactgctt attttattca gcaaaaaatg actgtgctct ctgatgaaga
     9061 atggctgaaa actagaggtg agcagaatgg aaatcgaaaa gaccaatcga atgaacgcgc
     9121 tctttgaatt ttattcgacc ttattgacag aaaaacaaat gaactacatg gaattatatt
     9181 atgcagatga tttttcatta ggtgagatcg cggaagaata cgaagtaagc cgacaagcag
     9241 tatatgacaa tatcaagcgg acaagcaaga tcttggagga ttatgaaaaa aaactccatc
     9301 ttttttctaa ttatattgtt cgagaacagg tattggaaaa tatgactgca tatattacag
     9361 ataagtaccc ggaagataaa aaactacttg aatatgtaca gcaaattcaa gcaatcgaag
     9421 aataaataaa aggaatgaag aaaatatggc atttgaaagc ttaactgaac gtctccagca
     9481 ggcgatggga aaaataagaa aaaaaggaaa agtatcagaa gctgacgtaa aagaaatgat
     9541 gcgggaaatc cgccttgcct tactagaagc cgatgttaac ttgcaagtag tcaaagattt
     9601 tactaagcgc gtacgcgaac gtgcggtagg ggcagaagtg ctggaaagtt tatcacctgc
     9661 acaacaaatc gtaaaaatcg ttgatgaaga attgacgatc acacttggtt ctgaaacagc
     9721 agagttaaat aaatcaccaa aaataccaac tgttatcatg atggctggtc ttcaaggggc
     9781 aggtaaaacg acctttactg gtaaattagc caattattta aagaaaaatg aaaatgctcg
     9841 tccgctccta atcgcagggg acgtatatcg accagctgcg atcgaccaat tgaaagtatt
     9901 aggtcaacag ctagacgttc ctgtatttga tatgggaact gaagtcagcc cggtagagat
     9961 cgtccgtcaa gggatggagt tagcaaaaga aaagaaaaac gactatgtat tgatcgatac
    10021 ggcaggacgt ctgcatatcg atgaaacatt gatggatgag ttgaaacaaa tcaaagaatt
    10081 gacgcaacca aatgagattt tgcttgttgt cgatgccatg actggtcaag atgccgtcaa
    10141 cgtagctgat agcttcaacc agcaattagg tattacaggg gtcgtcatca ctaaacttga
    10201 tggggatact cgaggcgggg cagcactatc gatccgctca gtgacaggcg caccaatcaa
    10261 attcatcggt tctggtgaaa aattgactga cttagaagta ttccacccag atcgtatggc
    10321 tagccgtatc cttggtatgg gggatatgct gacattgatt gaaaaagcgc agcaagacta
    10381 tgacgagaaa aaagcggaag aacttgcaca gaaaatgcgg gaaaattcat tcgatttcaa
    10441 tgactttatt gaacagcttg atcaagtgat gggtatgggc ccactggaag atctaatcaa
    10501 aatgatccca ggaatgaatc aagtccctgg aatcgaaaac gtcaaggtag atccaaaaga
    10561 tgttgaacgc aaaaaagcga tggtttattc tatgacgcca gcagaaagag caaatcctga
    10621 tctgttgaac ccaagccgtc gtcgccgaat tgctgccggt tctggaaaca gtgttgttga
    10681 agtgaatcga atgatcaaac aattcaagga atcaagaaaa atgatgcagc aaatgtccaa
    10741 aggggatatg aacatcccag gtatggacca aatgtttggt tcaggcgtca aaggaaaact
    10801 aggcaaaatg gcgatgaatc gaatgatcaa gaaaaataag aagaaaaaga aaaaacggaa
    10861 ataagatgaa aaaaacagct tcggtacaga ctgaaaatgt ctactgccgg agcttttcta
    10921 tcagcaattt ttattcttta ctaatacaat tactctaaac gaagaggtgg cacagatgag
    10981 atgtccgtgg agtgaacaaa cagaaagtat gcggcaatat catgatactg tctggggtgt
    11041 tcctgaatat gatgaccaaa agctgttccg aaaattgatg cttgatatta atcaagccgg
    11101 attgagctgg cagacgattt taaataaaag tgaagcattc gatgaagcat atgatcgatt
    11161 cgatattaaa aaagtcgcaa attatggaga aacaaaaatc agtgaactaa tggaaaacaa
    11221 aggaattatt cgtaaccgaa gaaaaatcga agcagccgtc caaaacgcca aagcagtctt
    11281 gaaaattcaa gaagaaatcg gcagtttttc agagtatctt tggtcgtttt ctaatggaaa
    11341 aatcgttgat tcttcttatc attcacaaga agaagtccca acaacaagtc ctctttcaga
    11401 tgaattagca aaagatctaa agaaaagagg atttaaattt atcggaagca caacaatcta
    11461 tgctttttta gaagctgtcg ggatcatcaa tgatcacctt gacagttgtt tccgcaaaga
    11521 agaggtcgta cgtatcggca gaaagggcga ataaagcatg cgaaaatcat attttatggt
    11581 gctataagtt tagatggcta tttggcaacg aaaacggatg atcttcaatg gctttttgac
    11641 actccaacag gtaagcaaac cacttatgaa gctttttatg aaacaatcga tacgaccatc
    11701 atgggcagaa aaacttatca ggaagcaaaa aaatatttgg agaccgaagc aatttatcca
    11761 gagaaaaaga atttcgtttt ttccacccac gcacaattac aattgccaga tgctgaggtc
    11821 gtaaacgaag atcctgttgc ttttctaaaa aatctacaaa tgacagaagg aaaagatatc
    11881 tgggttgtgg gtggcggtcg attgatcaaa ccgctattgg aacatcaaat gattgatgaa
    11941 tggtatatcc agattacccc tgtattatta ggggacggga ttcctttatt ccaaaaagga
    12001 acatatgaag cgcgttttga aatgctagac acgacacgat tcggtgaatt tatcgaattg
    12061 cattatgtac gaaagtagaa gtaaataaaa taaaaaaggc aactatatgc agaaatgtcc
    12121 tgtacatagt tgcctttttt attaccgtta tgctttttac tctgttaatt tgttagaaaa
    12181 atctagatac gcatctaaaa tcgtttggaa gaaacttaat gttccttcaa cgatattacc
    12241 attttcatca attagattcg caacgtttcc gatatatgct tccggttgct gcaaggttgg
    12301 cacattcaaa aagacaagtg attggcgtag atggtggttt gcgccaaagc cgccgatccc
    12361 acctggtgat gcagtaacga ctaaacctgg tttatgatcc caaacactgt gtccataagg
    12421 tcttgagcct acatctaaag catttttcaa tactgctggc acagaacggt tgtattcagg
    12481 agtaacgaaa acgacaccgt ctagtcccct cacttcctca cgaaaacgag tccattcagc
    12541 aggagcctgt tctggagttt cgagatcctc attgtaaaat ggcagatcat cgatttttac
    12601 aaatacactt tcgtaccctt caggaagcaa tttcgcaaaa gcttctgcta cttttttgtt
    12661 gtaagaatct tttcttaaac taccaataaa aaatccaatt ttttttgtca tacgattttt
    12721 cctccttcaa actatctata ataatcttac aaaagataag taactggagc aaataaaaac
    12781 acttactgac aaaagctaca tagaagatag attacttatt tttaacaaaa aagaagtcta
    12841 aaatcttcta ttttctaaaa aaaatcgaat ttaatcaagc tgtaaagaaa aaaagcttta
    12901 cagggtaaaa aaaaactgtt acactacatc ttgtgaataa aacaatggag gtggaaataa
    12961 ataatggcag ttaaaatccg tttaaaacgt atgggttcta aaaagagtcc gttttaccgt
    13021 atcgtcgtag ctgattcacg ttctcctcgt gatggacgtt tcatcgaaac tgtaggtact
    13081 tacaatcctt tgaaagaccc tgcagaagta gttttaaaag aagacttagt tcttgactgg
    13141 ttatcaaaag gtgcacaacc ttctgacact gtacgtaaca tcctttcaaa agaaggcgtt
    13201 atgcaaaaac accacgaagc taaattctca aagaaataag gtgaccggat atgaaagatt
    13261 taagcgactt agtcttaaca atcgttcgtc cgctagtcac ttatcctgat caagtcaagt
    13321 tagacgtcgt agagtcgaaa gacttttacg agtacaatct aacagtggct cctgaagata
    13381 ttggccgtat cattggaaaa caagggcgtg ttgctaaagc aatccgcaca atcgtttacg
    13441 gtgttcggat caatgcaccg aaaaaagtgc gtttgaatat catcgatgat aaggaaaagt
    13501 aaataaacgt gctgtactcg aattttcgag tgcagctttt ttgttttttt tattaattga
    13561 agaaatgatt atttggcaaa acaagcaaga aagcgtatcc ttataggagg agggagtgaa
    13621 cagccaatga gaaagaaaat gttatttatt ccatttgctc tattgttaag cgcttgtagt
    13681 tcggttactc aagaatcaga caatacacat ctatcaagca gtatatctga aactgttacc
    13741 agtccagaaa aaagtagtac caaaacggct tccaccactg aacaaacaat ggctacctca
    13801 aataatgaga aaaagactgc tttagaccag ttgaaagaac aacagccaaa tgttcccatg
    13861 cctctagatg taccagtctc ttcaggctat ctcaatatcg cagcgacaca tacaaagcaa
    13921 ggttattcta ttttatatta cagaacagat cgtcccctcg ggttgaatgc tgacgaattg
    13981 aatcaagaaa cgcctattgc aacctatcta tatcaatatg ggtttgcttc tagtcaagaa
    14041 acaatccaag ttttgcagcc atttgagatc gacacaaatg gtcagcaagt ggacttaggt
    14101 tctcgaatca cagggtatca gcaaggtgca gcaggttcaa gttttcttga atggcaagaa
    14161 ggaaactggc gtattcgaat ccgtgggaat aatatagaag gccaagatcc cttactatta
    14221 gctaaagaaa tcgtagctta tttggaagaa aacagtctac cagcacctga acaatttggt
    14281 aaaattactg ttgatatggg tgatacgacg aatcgagccg tagaagtaag ctggcaagaa
    14341 ccgaaaaatg cctatacgat cactcatcaa gatcctatgt ctgcgttgaa gatggcagtt
    14401 tctatgaaga ggttgtgaaa tcagcgcgct gatctgagcc catcatttaa ctccaagtca
    14461 caggaagtaa tcaaaaatag cacgccgcat ttttgcataa ttttggctta ataccttaaa
    14521 agaaaactag aattgcctct tcacacatgc taaaataaaa cagacaaaaa agaaatgggg
    14581 atataagttg actgaatact tgaatgtagg aaaaatcgta aacacacaag gtatcaaagg
    14641 cgaagtccgt gttatctcaa caacggattt cccagaagaa cgttacaaaa aaggaacggt
    14701 gctaacacta ttccaagacg gaaaagcacc tgtggaatta acagtcaaaa gtcatcgcaa
    14761 acataaaaat tttgacttat tgagttttga aggacatcct tctatcaatg atgtggaaaa
    14821 atatcgggat ggcatattaa aagtgtcaaa agacaactct gtagatctag ctgaaaatga
    14881 attctattac catgagatca tcggtctgca tgtattagaa gatggaactg agttaggtaa
    14941 agtaaaagaa atcttgtcac caggtgccaa tgatgtttgg gtcgttcaac gtccgaaaaa
    15001 agctgatata ttgcttccat atatcgcttc tgtcgtcaaa gaagtcgatt tagaagctgg
    15061 agtggtccgt gtagaaattc cagaaggact gattgacgat gaagattgat gtattgactt
    15121 tatttccaag aatgtttgaa ggtcctatgg gggaatccat catcggtaag gctgtagata
    15181 aaggattatt agatatcaat atctctaact tccgcgatta ttcagataac aaacatcaga
    15241 cagtagatga ttatccatac ggcggtggag caggtatgct gctcaaagtc cagcccatct
    15301 atgataacat ccaagccatt gaaaaagctg caccggatgt caaaaaaaga gtaattcttc
    15361 ttgacccagc tggtaaacga ttcgatcaga aaatggcaga agaattttca caagaagaac
    15421 acttagtatt catttgtggt cattatgagg gatatgatga acgtatccgc acattagtga
    15481 cagatgaagt ttccttagga gattatgtcc ttactggggg agaattaggt gcgatggtca
    15541 tgatcgatgc aacagttcgg cttttgccag atgtgttagg gaatcagact tcagctcaaa
    15601 cggattctta ttcaaccggt ttattggaac atccccaata tactcgcccg gcagaatata
    15661 aagggatgaa agtaccagaa gtattgacaa atggaaatca taaattgatt gaagaatggc
    15721 aattaaaaga atcactaaga aggacctatc aacgtcggcc tgatcttttg gaaacacttg
    15781 aatggacacc tcaaatgacg aaatttttag aagaaatcaa gaaagaagaa caagaaaaaa
    15841 cagcagatga agccaagtga ctttttctaa aatcttcttt acagtaggtt gataatatgt
    15901 tagtatttta tggtgagtgc aaaagcactt aactattacg atattccgct gtgagaaaag
    15961 ctcataagaa tatttggaat aaggagaaga aaaccatgaa tccattaatc gaagaattaa
    16021 caaaagaaca attacgttct gacatcccag cgttccgccc tggtgacact gtgcgtgttc
    16081 acgcaaaagt tgttgaaggt actcgtgaac gtatccagtt atttgaaggt gttgttatca
    16141 aacgccgcgg tgctggaatc agcgaaactt atacagtacg taaagtttct aacggagtgg
    16201 gcgttgaacg tacattccca ttgcatacac cacgtgttgc aaaaatcgaa gtagttcgct
    16261 acggtaaagt acgtcgtgca aaattgtact acctacgtgc attacacgga aaagctgctc
    16321 gtatcaaaga aatccgtcgt taatcattgt tatcagactg agaaagaacc cttggtattc
    16381 aagggttctt ttttcttagc atgtaatttg aacaccctga tccagttttt tctttaatta
    16441 ttggtagggg agaataatgg attagaataa atctacctat aagtagtagt aaaaaaaaga
    16501 gcactatgat aatatctttc ttaagagtaa ccgctttaat aaaaaatcca gaatattcta
    16561 acataaatgg agtattctta gaatcaaaag gagaaagaag atggtttata cttatacgga
    16621 gcaagaaaag atgaaagttt tagacacatt catgaatgat ggaaaagtaa aacaagtacc
    16681 aagttcaaca aaaaagaaat acatcctatt gaaagaagtc ttgaaacgct ttgaccatgg
    16741 agtgacttat acagaaacag aggttaattc gatactgctg aacgtatttt ctagtggcga
    16801 ttatgtcgaa caaagacgtt atctaatcac atttggcttt ttcaaaagaa gttcagacgg
    16861 tcgggcatat cagatgatgg gaattgaaaa ttagaagttt taatttatac tcattgaaaa
    16921 aagttcaagt atgcgatatt ggttctacac cgcatacttg aacttttttt gtcatctgta
    16981 tctataatac aaagccaaga acgttattca aaatatggat agctatgata aaagctaatg
    17041 agcgattcgc agttaaataa gcaatcgtca gtaaaatcgt tccaggagcg tacatgaaaa
    17101 aatcaattgg atgcgttaca tgaataaaga taattctctt ttatttgtac gaataaatac
    17161 caaaacgtac ctcctaaaat tagattttgg acagatgccc tttaaaaaga taacaagatt
    17221 tcgttcttga aacaatatat atgcttttat tccaatgagg ttgtgaaaac agtattttgt
    17281 cctgagcgtt agaaaattga aaatggggaa tagcaactcg tactttacca ttttttaagt
    17341 gaatacaaga tcagctaatt gaacaccgtt tattcgtgat gagggacaca acactgcagt
    17401 cgacacattt ctcttttatg ttcaaagata acaaatattt aaaacaaaga aaaatagtag
    17461 ataatcccgt cctaactctt t
//
LOCUS       DAIGOK010000057        17435 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.057, whole genome shotgun sequence.
ACCESSION   DAIGOK010000057 DAIGOK010000000
VERSION     DAIGOK010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17435
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.057"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..134
                     /locus_tag="NOA01_001504"
     CDS             <1..134
                     /locus_tag="NOA01_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCI0632702.1"
                     /translation="VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            238..2514
                     /locus_tag="NOA01_001505"
     CDS             238..2514
                     /locus_tag="NOA01_001505"
                     /inference="COORDINATES: protein motif:HMM:NF024867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCI0632703.1"
                     /translation="MSLKLKGIKIDSFRAFERQKYFDCVNPVFNEISNLIVLYAPNGT
                     GKTSFFDAIEWSLTGEISRLNENKNVRDIASQERKYILKNKYSSAEVDSVELAFDDGS
                     FYALTTKRLNGKQTTDYVEGNETKKTVDLTAKELKILRQKNLLTHNQIDAFLRSQSAE
                     GRYDALSNFWDKENVSEDYKNLVLFVNEMNKVLGQQETKMNQLTKEIEKLESNAKIWQ
                     TINQLKENYAKLNGKNSKKDPLLKNQLGLQELYIESNKLKSNNQIKLNQFEEERQQLE
                     NLITMIQEYRQIPQQIEWSEKQVADVNEKLAHLTEIETIEEKKKALFDEKIDLSNHLK
                     RCNFLINHEADFQALEMAEQNSNREINEWINQRKKCEQALITLQKEHQEQEVTLSECQ
                     NKRALRTNQLTEIKELNSYSDVKAKRQLLDHEIKQQKKKKIGLEAKLGKIKLTLTKLF
                     SLLNLDDHQLSQKDYSKDPDYSFLLEQISQLDDIWQERKKLEVDLTQKKKERSSLEDL
                     GSQLEQLKKLGYSIVTDTSSSVCPLCLHDHQEFQHIIQKIEASTADLLPIEQLTKEIS
                     HLEGMIDESTRRLTEVVSGVKQQIQIQITVKETEINQLDQDLLQVTLKLKQQEEAYLT
                     NEKHWYKLNEYSQKYGIDCEKIAEETQKRVKSLQEELVKLTNEYQNIKEKINQNEQLN
                     VKNTHTLKTIELHYQQAKKSLNDLKGNEQYQHYQQEQAFYEALGISVISDFKRQCKRE
                     LEKINEQETKLIAEKNND"
     gene            2661..3350
                     /locus_tag="NOA01_001506"
     CDS             2661..3350
                     /locus_tag="NOA01_001506"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCI0632704.1"
                     /translation="MEKNKQKLTTAIYKLNEKNQLLGQLSGLTSDFISETGIETKRSE
                     QAKVKKIYDHLSRKVQELKELKDSKLTEIKKQIKSTFNLYTVNQIFQMIEPHPDFRKI
                     SFKINEANPDKLGLDIMCKRTEDEEDAPILYLSSAQVNILSLSIFLGTALENTDKVNT
                     IFMDDPIQHLDSLNELSFIDLLRILAFKLGTQIIFSTHNRQFFDLCQRKLDANYHNAS
                     FIEMNYTENQL"
     gene            3696..3884
                     /locus_tag="NOA01_001507"
                     /pseudo
     CDS             3696..3884
                     /locus_tag="NOA01_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4139..4870
                     /locus_tag="NOA01_001508"
     CDS             4139..4870
                     /locus_tag="NOA01_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCI0632705.1"
                     /translation="MGNSFKNKALYHQLVDLLKNQMESNMIPHDKLPSERELTAQYGV
                     SRTTVRLALKELENTGYIYRRHGKGTFVSDIKKNVADLAGTYSFTEQMKSLGRTAETR
                     ILAFEKIEADKFIAKQLNLSLGEVVFKLSRLRIADREPLMIEDTYLPVKFFLSLNDQL
                     LRSKPLYDLFSEDFNQTVRLADEELYASVASKEDAKQLIISEGAPVLHLARQTYNMKN
                     EIIEFTLSVARADQFHYQIRHIRNS"
     gene            4883..6055
                     /locus_tag="NOA01_001509"
     CDS             4883..6055
                     /locus_tag="NOA01_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCI0632706.1"
                     /translation="MFTVGKEKLDQLGAEITTREIRQQPELWEETLDHYQTVQKELDN
                     FLAIVKEKANGQRTRVIFTGAGTSQYVGDTLVPYLRKNGDRQAFSFESIGTTDIVAEP
                     EEYLIKEDPTLLVSFARSGNSPESLAAVEIANQMVDTIYHLSITCAVEGKLAQISQSD
                     SNSFLFLMPARSNDSGFAMTGSFSCMTLGALLTFDQTVLVKKQQYLKVLNNLGNEVIT
                     RVNEIEKIVQLDFERIVYVGSGSLAGLTREAQLKILELTAGKIATIFDSSMGFRHGPK
                     SFINQKTIMFGFVNNNPYTRDYDLDVLEEVRGDEIASGVFAITQPGQRNFTGDKFEFT
                     TEAELLPDGYLALSYIMVAQIVALLSSIKVNNKPDTPSPTGTVNRVVKGVTIHKYK"
     gene            6078..7085
                     /gene="lacD"
                     /locus_tag="NOA01_001510"
     CDS             6078..7085
                     /gene="lacD"
                     /locus_tag="NOA01_001510"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016181191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCI0632707.1"
                     /translation="MITLTRGKKAAMDRLSTKDGIISALAIDQRGALKKMIKALNVEL
                     NDEQIERFKELVSSELTPYASSILLDPEYGLPAASARHEDAGLLLAYEKTGYDVTTPG
                     RLPDLLADWSVLRLKEQGADSIKFLLYYDVDEDPEINHQKHVFVERLGSECAEEDLPF
                     YLELLSYDTQISDVHSLEYAKVKPHKVNSMMKEFSKPQYKVDVLKVEVPVNMNFVEGF
                     STDEVAYTEEEAAKYFLEQSQATDLPFIFLSAGVSTKLFQQTLFFAKDSGSTFNGVLC
                     GRATWKNGVKPFIKSGEESARAWLKSEGRENIESLNNVLAQTASSWYDKVQVEKEAAV
                     N"
     gene            7097..8035
                     /locus_tag="NOA01_001511"
     CDS             7097..8035
                     /locus_tag="NOA01_001511"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HCI0632708.1"
                     /translation="MIVTVTMNPSIDISYPLASLNVDTVNRTDQVSKTAGGKGLNVTR
                     VIHDLKGDVLATGVLGGYHGAFIADELKHAGIKQKFTDIKEESRDSIAVLHEGNQTEI
                     LEAGPTVSYEEQQNFLENFKELIKKAKIVTISGSLAKGLPSNFYQTLVQLTEEQSAKV
                     LVDTSGESLHQVLAGKQKPFLIKPNLEELSTLLRHSFSIDRLEEVQEELIQPIFAGIE
                     WIIVSLGKAGALAKHKDCFYRVTLPTIKAVNPVGSGDATVAGFAYGLSQEMDEIELLK
                     FCMATGMANAQEGQTGHVSPVNIQSHIEKITVKKIE"
     gene            8869..10647
                     /locus_tag="NOA01_001512"
     CDS             8869..10647
                     /locus_tag="NOA01_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HCI0632709.1"
                     /translation="MNTFEIKEDFLLNGKPFKILSGAIHYFRVDSADWYHSLYNLKAL
                     GFNTVETYVPWNLHEPKKGDFHFEGILDLEHFLSIAEELGLYAIVRPSPYICAEWEFG
                     GFPAWLLNEGTRIRTNETVYLNHVADYYDVLIKKIVPHQLTNGGNILMIQIENEYGSY
                     GEEKDYLRSIRDLMLDRGITVPFFTSDGPWRATLRAGSMIDEDILVTGNFGSKAEENF
                     SSMEAFFNEHGKKWPLMCMEFWDGWFNRWKEPIVQRDAKELAEAIKEVVLRGSINLYM
                     FHGGTNFGFMNGCSARGVIDLPQITSYDYGAPLDEQGNPTEKYYAIQTMIHETFPDIQ
                     QMEPLTKDTMEMKDIPLIDKVSLFSTLDTISQSVKMKYPETMEMLGQNTGYILYRTSI
                     KKDAEHERLRVIDGRDRSQLFLNQKLQATQYQTEIGEDIIVPMPQEDNQLDILIENMG
                     RVNYGHKLLADTQKKGIRTGVMADLHFITDWNQYCLPLESCEKVDFSKEWHPNQPSFY
                     RYEVTLDEVEDSFIDLSKFGKGVVFVNQTNIGRFWEVGPTLSLYIPKSLFKKGNNEIV
                     IFETEGTFQPEIQLVKAPLYKEMKEG"
     gene            10651..11130
                     /locus_tag="NOA01_001513"
     CDS             10651..11130
                     /locus_tag="NOA01_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCI0632710.1"
                     /translation="MSIIGVRIDGRLIHGQVANLWTTKLNITRIMVVDDEVAKNDIEK
                     SGLKLATPPGVKLSVLPIEKAANNILAGKYDSQRLLIIAREPDRLLKLVELGVPIEEI
                     NVGNMSQTNETRSITKSINVVDQDVEVFNKLQAKGVRLVAQMVPSDKAENFMALLAK"
     gene            11143..12039
                     /locus_tag="NOA01_001514"
     CDS             11143..12039
                     /locus_tag="NOA01_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCI0632711.1"
                     /translation="MDILWWQILLLTLYAGYQILDELQIYSSLSAPVFAGLFAGLVMG
                     DLKAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSVALGMNPEQAIAVIAVP
                     VASLMIQLDILARFANTYFAHRIDKLVEDMNYKGIERNFLMGALSWSLSRMIPVFLAL
                     AFGGGLVEQVVVVLNGDLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHFPYLILGFT
                     LTALLGTVFTNMQVLGTGVAGAVKNFEGIFNSLPMLAIALVGFSLAAISYKNGQLIPH
                     SPAPKDVTSNDSDEGEIEDDEI"
     gene            12026..12835
                     /locus_tag="NOA01_001515"
     CDS             12026..12835
                     /locus_tag="NOA01_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCI0632712.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKLYGDNSPELKEMMKTHTQFFNTSNFFNTIVTGIDLAIEEKEGIDGKDTVSGLKA
                     GLMGPFAAIGDSIFAALIPTIFGALAANMAINGNPTGIFIWIIAQIAVMIFRWKQLEF
                     AYREGISLVTTMQHRLTALTDAATLLGVFMVGALVATMINVKLSWAPSIGDVTLNMQN
                     NLDMILPRLLPAGIVAAIYWMLGKKNMTSTKAIFIVLVVCVALSALGVISK"
     gene            12873..13277
                     /locus_tag="NOA01_001516"
     CDS             12873..13277
                     /locus_tag="NOA01_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCI0632713.1"
                     /translation="MGKQLVLISHGLFCEELKKSTEMIMGPQESIHTVSLLPSESAED
                     FREKFETVIANLDDFVVLADLMGGTPCNVVSKIILEGQVIDLYAGMNMPMVIDFINSQ
                     LIGTELSLIESATSNICYVNGRIFSDDDDEDE"
     gene            complement(13750..14631)
                     /locus_tag="NOA01_001517"
     CDS             complement(13750..14631)
                     /locus_tag="NOA01_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCI0632714.1"
                     /translation="MTFAIYAYLKEKNNLFLWISLYLAFFIFDNTIIYMTEFLNSFAQ
                     SYDQVFMSAPAVKTIIFMGNAFFSISILAELQKEKLSPLHYALLLALAVWMITIPLMD
                     NSAFKVWLYYLGNQLFLFYLGIYCWRGTKKDVPLLNKHYLKQLMFINLFFSILIIIED
                     SFVIFNVDQYSSLVTKIYNRSVSEDIFSIVICVLLLHFFIKKYQPQKEKTQKQDTSLL
                     IQDFCQDHQFTQRETEIFILLLSHRTNQEIADQLFLSLGTVKTHVHNIFIKLDIKKRT
                     QIIPLFEKYKEAIESTV"
     gene            14957..15976
                     /gene="ptcA"
                     /locus_tag="NOA01_001518"
     CDS             14957..15976
                     /gene="ptcA"
                     /locus_tag="NOA01_001518"
                     /EC_number="2.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine carbamoyltransferase"
                     /protein_id="HCI0632715.1"
                     /translation="MGKKDFITTENYTKEEIQQIVDLSLKIKAAIKNGFYPPLLKNKV
                     LGMIFQQSSTRTRVSFETAMTQLGGHAEYLAPGQIQLGGHETIEDTSRVLSRLVDILM
                     ARVERHHSVYDLATNATIPVINGMSDFNHPTQEIGDLCTMIEHLPAGKKIEDCKVSFI
                     GDATQVCFSLGLIATKMGMEFVQFGPEGFQLNEDHRARLDAICKDSGGSYTISDQIDV
                     IEGSDFVYTDVWYGLYEAELSEEERMRIFYPKYQVNTDLIKRAGKQAKFMHCLPATRG
                     EEVTDEVMDGPNSICFDEAENRLTSIRGLLVYLMRDYAEKNPIDETKKAAAKKDLEAL
                     LGETF"
     gene            16213..17250
                     /locus_tag="NOA01_001519"
                     /pseudo
     CDS             16213..17250
                     /locus_tag="NOA01_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355588.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
BASE COUNT         5972 a         2768 c         3526 g         5169 t
ORIGIN      
        1 aagtgaatca gacatttatt tccagtgtca gcaatcaacg taatcatatt ccaagaaaat
       61 cattgaatta cagaacacca atcgaaatat ttttgagcta tgtacaagaa gcattttatt
      121 ctagcttaat ttgacaaatc atagaattaa aaacgatggc taaaaaagag aaaaaagcac
      181 gaatacaaga gtatataaaa catgagctgt caaatcttaa cgtaaaggaa ggaaccgatg
      241 agtcttaaat tgaaagggat aaaaattgat tcgtttcgag catttgaaag gcaaaaatat
      301 tttgattgtg tgaatcctgt ttttaatgaa atatcaaatt tgattgtttt gtatgcgcca
      361 aatggaaccg gaaaaacctc tttttttgat gccatcgaat ggagcttgac tggcgaaatc
      421 agtcgactga atgaaaataa aaatgtccgt gatatcgcct cccaagagcg aaagtatatt
      481 ttaaagaaca aatattcctc agccgaagtg gacagtgtgg aactagcatt tgatgatggc
      541 agtttttatg cattaacgac gaaaaggtta aatggaaagc aaacgacgga ttacgttgaa
      601 gggaatgaga ccaaaaaaac ggttgattta acagcgaaag aattaaagat tttacgacaa
      661 aaaaatttat tgacacacaa tcaaatcgat gcttttttac gatcgcagag tgccgaagga
      721 agatatgatg ctttaagtaa tttttgggat aaagaaaatg tatctgagga ttataaaaat
      781 ctagtcttat tcgtcaatga aatgaataaa gtattaggac aacaagaaac taaaatgaat
      841 cagctaacaa aagagattga aaaactcgaa agtaatgcaa aaatatggca aacaatcaac
      901 cagctgaaag aaaattatgc caagttaaac ggaaagaata gtaaaaaaga tccattgtta
      961 aagaatcaat taggtttaca agaattgtat atcgaatcga acaaattaaa aagcaataat
     1021 caaataaaat tgaaccagtt tgaagaggag cgacaacagc tagaaaattt aatcacaatg
     1081 atccaagagt atcgacaaat accgcagcag atcgaatggt ctgaaaagca ggttgctgat
     1141 gtcaacgaaa agttagccca tttgacagaa attgagacaa tcgaagaaaa aaagaaagca
     1201 ctgtttgacg aaaaaataga cttgagcaac catctcaagc gttgtaactt tcttatcaat
     1261 catgaagcag attttcaggc actagaaatg gctgaacaaa acagtaatag agaaatcaat
     1321 gaatggatta atcaacgaaa aaaatgtgaa caagcattga ttactttaca aaaggagcat
     1381 caagagcaag aagtaacatt aagtgagtgt caaaataaac gagcgttgcg gacgaatcaa
     1441 ttaacagaaa tcaaagaatt aaacagttat tccgatgtta aagcgaaaag acaattactt
     1501 gatcatgaaa tcaaacaaca gaaaaagaaa aaaatagggt tggaagccaa gttaggtaag
     1561 ataaagctaa cgttgacaaa attgttttca ttactaaact tagacgatca tcagttaagc
     1621 caaaaagact attcaaaaga tcctgattat tcatttttgt tggaacagat aagccaactt
     1681 gatgacattt ggcaagaaag aaaaaaattg gaagttgatt taacacaaaa gaagaaggaa
     1741 cgttcctctt tagaagatct gggcagtcag ttagaacagt tgaaaaaatt aggttattct
     1801 atcgtaacgg atacgtcttc gtctgtctgt ccattatgtc tccatgatca tcaagaattt
     1861 caacatatca tccaaaaaat tgaagctagt acggcagatc ttttaccgat tgagcaactg
     1921 acgaaggaaa tcagccactt agaaggaatg atcgatgaat caactaggcg gttaactgag
     1981 gtagtctctg gagtaaaaca acagattcag atacaaataa cggttaagga aactgaaatc
     2041 aaccaacttg accaagatct gttacaagta accttaaaat taaaacaaca agaagaagcg
     2101 tatttaacca atgagaaaca ctggtacaag ttaaatgaat acagccaaaa atatgggatt
     2161 gactgtgaaa aaattgctga agaaacccag aaaagagtaa agtcactaca ggaggaattg
     2221 gtaaaattaa cgaatgagta ccaaaatatc aaagaaaaaa tcaatcaaaa tgaacagctg
     2281 aatgtcaaga atactcatac gctcaaaacg atcgaattac attatcaaca agcgaaaaaa
     2341 agcttaaatg atttaaaagg aaacgaacag tatcaacatt atcagcaaga acaggcgttt
     2401 tatgaagcgc ttggtatttc tgttatctct gattttaaaa ggcaatgtaa acgcgagtta
     2461 gaaaaaatca atgaacaaga aaccaagctt attgccgaaa aaaacaacga ctagaacaag
     2521 taaatgggaa aagtaaaaca gaattagtag aagaacaacg aatccaagca gagcagattc
     2581 ggcagctcgt tgtacggagg aaatatatcg aaaagatatc ttatcaactg tttgaccaag
     2641 taatcattgc accagaagaa ttggaaaaaa ataagcaaaa attgactaca gctatttaca
     2701 aattaaatga aaaaaatcaa ctgttgggtc aactatctgg gcttaccagt gattttatct
     2761 ctgaaacagg tatcgaaacg aaaagaagcg aacaagcaaa agtaaaaaaa atctatgacc
     2821 acttaagtag aaaagtacaa gaactaaaag aattaaaaga cagtaaatta accgagatca
     2881 aaaagcaaat caaaagtaca tttaacttat ataccgtcaa tcagattttt caaatgatcg
     2941 aacctcaccc tgattttcgt aagattagtt ttaaaatcaa tgaagcaaat cccgataaat
     3001 taggactgga tattatgtgc aaacgaacag aggatgaaga ggatgccccg attttatatc
     3061 taagttctgc ccaagtaaat atattgtctt taagtatttt tctaggaacc gctctagaga
     3121 atacagacaa agtgaatacg attttcatgg atgatccaat ccaacactta gatagtctta
     3181 atgaattatc ctttattgat ctattaagaa tccttgcatt taagctagga acacagatca
     3241 tcttttccac ccataaccgc cagttttttg acttatgtca gagaaaatta gatgccaatt
     3301 accataatgc cagttttatc gaaatgaatt atacagagaa tcagttgtaa caaatttagt
     3361 tagtcagaaa aagtgaataa gggagctctt tgatgagagc tgaggttagc tgaaagattc
     3421 agagtataaa acaaaaattg cctacagaag taaataaaga gacgaataga gaatcaatcc
     3481 caacgaataa gggattgatt ctttttttct agattaaata cagataggaa gaatatatag
     3541 attggatgga gagttgtact acctatacga aagcagaagc ttttttaggc tctacacttt
     3601 ttgatattgg cgaaattcgc caacattttt ttatgattta aacggtatag aaaagacatc
     3661 attcactatt tgggggctat ttaaccagag aaaccgaaac ctatacgaca actttgcgaa
     3721 catttagaaa gtttctgaca cagatccgaa atgccctccg ctacaattac tcaaacggat
     3781 tactagaatg tttaaacaag cctatcaaag tatttaaacg aaatttttat catttcaaac
     3841 ggcgaatcat gattcgtcac gataaaactt ttttaagcaa ataaacatac taagattagt
     3901 aggtttctat ttcctgtttt tttcaactgg tatcagattt cagcacttat gactgttatt
     3961 ttcgctagga acaatttctt aaaaatggat agttttatta tttcaataaa ataatcattg
     4021 ctaaatcaac gatttaagac ggttgtagaa tttttttcag attgtttgtt gacaaaaata
     4081 aaaaaaccat cttaccatta gtttgtgatt ataaccagtt taaaagaagg agaaacctat
     4141 gggaaattct tttaaaaaca aagctttgta tcatcagttg gtagatttac ttaaaaatca
     4201 gatggaaagt aacatgatac cccatgataa attaccctct gaacgagaat taactgctca
     4261 atacggagtt agtcgtacaa cggttcgatt agctttgaaa gagttggaaa atacaggtta
     4321 tatttatcgg agacatggaa aaggaacatt tgtatccgat atcaaaaaga acgtagcaga
     4381 tttagcagga acctatagct ttactgaaca aatgaaaagt ttaggtagaa ctgctgagac
     4441 gcggatctta gcttttgaaa aaattgaagc ggacaaattt attgcaaaac aattgaatct
     4501 ttcattagga gaagtagtct tcaaattaag tcgattgcgg atagctgatc gggaaccgct
     4561 tatgatcgaa gatacctatt tgccagtcaa atttttctta tcattaaatg atcagctttt
     4621 aagaagcaaa cctctatatg atcttttttc cgaggatttt aatcagaccg ttcgattggc
     4681 agatgaagag ctatatgcaa gtgttgcctc aaaggaagat gcgaaacagc tgataatctc
     4741 agaaggagca cctgtccttc atttagcacg tcaaacctat aatatgaaaa atgaaattat
     4801 cgaatttact ttaagtgtcg cccgtgcgga tcaatttcat tatcagatcc gccatattcg
     4861 aaatagttag gagaatcgaa acatgtttac agtaggaaaa gaaaaactgg atcagctggg
     4921 tgctgagatc acgactcgcg aaatacgcca acagcctgaa ctttgggaag aaacccttga
     4981 tcactatcaa acagttcaaa aggaattaga taattttctt gcaatagtta aagaaaaggc
     5041 aaatggtcag cgaacacgtg taatcttcac tggtgcagga acatctcaat atgtaggaga
     5101 cacattagtt ccttatttgc gcaagaacgg tgatcgccaa gcatttagct ttgaaagtat
     5161 tggaacgact gatattgtcg cagaacctga agaatattta attaaagaag acccaacttt
     5221 acttgtatca tttgcgagga gtggaaacag tcctgaaagc ttggcagcag ttgaaattgc
     5281 aaatcagatg gttgatacga tctatcattt atcaattacg tgtgctgtag aaggaaaact
     5341 agctcaaatt agtcaatccg attccaatag ttttttattc ttgatgccag cacgttccaa
     5401 tgatagtggc tttgctatga ctggtagctt ttcctgcatg actttaggag ctttgttgac
     5461 attcgatcaa acagtattag tgaagaaaca acaatacctg aaggttttaa ataatttagg
     5521 aaatgaagta attacccgag taaatgaaat tgaaaaaatc gttcagcttg attttgaacg
     5581 cattgtttat gttggttcag gaagtctagc tggattaaca agggaagcgc aattgaagat
     5641 tctggaattg acagccggaa aaattgctac catctttgat tcatcaatgg gctttcgcca
     5701 tggtccaaag tcgtttatta atcaaaaaac aatcatgttc ggttttgtta ataataatcc
     5761 ttacacgcgt gattacgact tggatgtctt agaagaagtg cgtggagatg aaatcgcttc
     5821 cggagtgttc gccattactc aaccagggca acggaatttc actggcgata aattcgagtt
     5881 tacaactgag gccgaattac tgccagatgg ctatttagca ctaagctata tcatggttgc
     5941 ccaaatagtc gctttgttat catcgattaa agtgaataat aaaccagata caccgtcacc
     6001 aacaggaaca gttaaccgag ttgtaaaagg tgtaacgatt cacaaatata agtaaaaata
     6061 aactgggagg aagaaccatg attacattaa ccagaggaaa aaaagcagca atggatcgtt
     6121 tatcgacaaa agatggtatt atcagtgcct tagcgattga tcaacgcgga gcattaaaaa
     6181 aaatgatcaa agccttaaat gtagaattaa atgatgaaca gattgagcgt ttcaaggagt
     6241 tagtttctag tgaattaacc ccttatgcat cttctatttt attggaccca gaatatggtc
     6301 ttcctgctgc aagtgcacga catgaagatg ctggtttgtt gttggcctat gaaaaaaccg
     6361 gctatgatgt aacaacaccg ggacgcttgc ctgatttatt agcagattgg tcagttttac
     6421 gattaaaaga gcaaggagcc gattcgatta agtttctact ttattatgac gtagatgaag
     6481 atccggaaat caatcatcag aagcatgtct tcgtagaacg tttaggtagc gagtgtgccg
     6541 aagaagattt gccattctat ttagaattgc tatcatatga tacccaaatt tcagatgttc
     6601 attcacttga atatgccaaa gtaaaaccgc ataaagtcaa tagcatgatg aaggaatttt
     6661 cgaaaccaca gtacaaagta gatgttttaa aggtagaagt tcctgtcaat atgaattttg
     6721 tagagggttt cagtacagat gaagtagcgt ataccgaaga ggaggcggca aagtacttct
     6781 tggaacaaag tcaagcgaca gatttgcctt ttattttctt gagtgccggt gtttctacga
     6841 agctattcca acaaacattg ttttttgcta aagattcagg ttcgaccttt aatggtgtgc
     6901 tttgtggtag agctacttgg aaaaatggtg tgaagccgtt tatcaaatct ggtgaagaat
     6961 ctgctcgtgc ctggctgaaa agtgaaggtc gtgaaaatat cgaaagcttg aacaacgttt
     7021 tggcacaaac agccagttct tggtatgaca aagtccaagt tgaaaaagaa gcagctgtga
     7081 actaggagga gagctcgtga tagtaactgt aacgatgaac ccgtcaattg atatttccta
     7141 tccgcttgct tccttgaatg tggatactgt caatcgaaca gatcaagtta gtaaaacagc
     7201 aggtggaaaa ggacttaatg taacccgagt aatccatgat ctaaaggggg atgtactcgc
     7261 tacaggagtt ctcggtgggt atcatggggc gtttattgca gatgaattaa agcatgccgg
     7321 tattaagcag aaatttacag atatcaaaga agaaagccgg gattcaatcg ctgttttgca
     7381 tgaaggaaat caaactgaga ttttggaagc gggaccaaca gtttcctatg aagaacagca
     7441 gaatttccta gaaaacttca aggagctaat aaaaaaagca aagattgtca caatctcagg
     7501 tagtcttgca aaaggattgc catcaaattt ttaccaaacg ttggtccaat tgacagaaga
     7561 gcagtccgca aaggttcttg tcgacacctc tggggaaagc ttgcatcagg ttttagctgg
     7621 caaacagaag ccatttttaa tcaaaccaaa tcttgaggaa ttatcgacac tactgagaca
     7681 ttcattttcg attgatcgat tagaagaagt ccaggaagaa ttgattcaac ctatcttcgc
     7741 aggtattgaa tggatcattg tttctctagg aaaagctgga gctctcgcaa aacataaaga
     7801 ttgtttttat cgagttacgc tccctacgat aaaagcagtc aacccagttg gttcaggaga
     7861 tgctacagtc gctggatttg cctacggcct atcacaagaa atggatgaaa tagaactttt
     7921 gaagttttgt atggctactg gtatggctaa tgcacaagaa ggacaaacag gtcatgtttc
     7981 accggtgaat attcaatcgc atattgaaaa aataactgta aaaaagattg agtgaaaaaa
     8041 tcgatctaga taaaatcaat gattttttga gaccaactca atttggtaga agttccattt
     8101 ttgtaggatt caacgtactc cagtagttgt tgtgaatctc tacgtttcgt cactcggact
     8161 tgcagaacgg tcgtttcatc ggtaagaatc tttaatgatt tcgcaacatt taaaggagtt
     8221 ggaaatataa ttttatttgt ttccgtaaaa tcttcctcat tcatgtggat attgtagtct
     8281 tgtttaaagc gctgacagct ggaattgtaa taacacaatt cttgacaatt aggatccaca
     8341 tattgttcgg gcaaataagt ttgctgatag atatatgcag ttttttagtt tttcgtaaat
     8401 gtctgatgta atcataaaat gctaaggctg gcagtttaag cttgtttaaa tagtgtggat
     8461 cattccctcg aacgattgat aaaacttgga cttgatcatt actgatagtg aataattcaa
     8521 tgtcagaaaa ctagaacagt ttttctttac gtgaaacaaa tgagtcacga ccttgatgac
     8581 gaattatgaa tccatccttt attcattcag ggagcgaatg actgtaatag agcttacgtt
     8641 aaatatttgt attaattctg cttctgtata aagtttattg ccattttggg atgtctaaat
     8701 taacccttca aacaacatca aagaattaaa cttttcgtta gagtctttgg ttttgtatgt
     8761 ataatttgat tttaacacgt tctgttatgt tggatatggt tgaatttcta tttcaaatta
     8821 tttaatatat caatatattg aaaacgcttt tatttaagga gggagctttt gaacacattt
     8881 gagattaaag aagattttct tctaaacggt aagcccttta agatactctc aggagctatc
     8941 cactatttta gagtggattc agccgattgg tatcactcgt tatataatct gaaagcattg
     9001 ggatttaata cagttgaaac ttatgtacca tggaacctac atgaacctaa aaaaggagat
     9061 ttccattttg agggaatttt ggacttagaa cattttttgt ccattgctga ggaattagga
     9121 ctatacgcga ttgttagacc atctccatat atttgtgcag aatgggagtt tggtggtttt
     9181 cctgcttggt tacttaatga gggaactcgg atccgaacaa atgaaaccgt ttatttgaac
     9241 catgtggcag attattatga tgttttaata aaaaaaatcg tgccgcatca gctgacaaat
     9301 ggtggaaata ttttaatgat tcaaattgag aatgaatacg gttcttatgg agaagaaaaa
     9361 gactatttac gttcgatacg tgatttaatg ttggacagag gaataacagt gccatttttc
     9421 acttcggatg gaccatggcg tgcgacactc agagcaggca gcatgattga tgaagatatt
     9481 ttagttactg gaaattttgg ttcaaaggct gaagaaaatt tttcttcaat ggaagctttc
     9541 ttcaatgagc atgggaaaaa atggccttta atgtgtatgg aattttggga tggttggttt
     9601 aatcgttgga aggaaccgat tgtacaacgt gatgcaaaag aattagctga ggcaatcaaa
     9661 gaggtcgtgc tgagaggaag tattaacctg tatatgttcc atggaggaac aaattttgga
     9721 tttatgaatg gttgctcagc tagaggagtg attgatttgc cacagattac ttcttatgat
     9781 tacggagcac ccttagacga gcaaggaaat ccaaccgaaa aatattatgc aatccagaca
     9841 atgattcatg aaacgttccc tgatatccaa caaatggaac ctttgacaaa agacacaatg
     9901 gaaatgaaag atattccatt aattgataaa gtcagtttat tttcaacatt agatacaatt
     9961 agtcaatcag taaaaatgaa atatccagaa acgatggaaa tgttaggaca aaatacaggt
    10021 tatattctgt atcgcacctc aataaaaaaa gatgcagaac atgaaagact gagagtaatc
    10081 gatggaagag accgtagcca actattttta aatcaaaaac tgcaagctac acaatatcag
    10141 acagaaattg gtgaagatat cattgttcca atgcctcagg aagacaatca attagatatt
    10201 ctaattgaaa acatggggcg cgttaattat ggtcataaat tattagccga tacacaaaaa
    10261 aagggaattc gtacaggggt catggctgat ctacatttta tcactgattg gaatcagtat
    10321 tgtttaccac tagagtcatg tgagaaagtt gatttttcaa aggaatggca tcctaaccag
    10381 ccaagttttt atcgctatga agtaacactt gatgaagtgg aggatagctt cattgatcta
    10441 tcaaaatttg ggaaaggtgt cgtatttgtt aatcagacca atattggacg tttttgggaa
    10501 gttgggccaa cactttcgct atatattccg aaaagcttat ttaaaaaagg aaacaatgaa
    10561 atagtgattt ttgaaacaga aggaactttc caaccagaga tccaattagt aaaagcacca
    10621 ctatataaag aaatgaaaga aggataaaca atgagtatta tcggagtaag aattgatgga
    10681 cgtttaattc atggacaagt agccaactta tggacaacca agttgaacat cacacggatt
    10741 atggttgtag atgatgaagt tgcaaaaaat gatattgaaa aaagtggatt aaagctggca
    10801 actccaccag gagttaaatt aagtgtattg ccgattgaaa aagctgctaa taatatttta
    10861 gcaggaaaat atgattctca acgattattg attattgctc gggagccgga tcgtttattg
    10921 aagttggtag aattaggagt cccaattgaa gaaattaatg ttgggaatat gtcacagaca
    10981 aatgaaacac ggtccattac taaatcaatc aatgtggttg atcaagatgt agaagtattt
    11041 aacaaattac aggctaaagg tgttcgctta gtagcgcaga tggttccaag tgataaagca
    11101 gaaaatttta tggctttatt agcgaaatag gaggataaat aaatggatat cttatggtgg
    11161 cagatcttat tattaacatt atacgcaggg tatcaaattt tagatgagtt acaaatttat
    11221 tcatcgttga gtgcacccgt ttttgccgga ttatttgctg gtctagtgat gggagatcta
    11281 aaagcaggac ttattattgg tggtagtatg caattgactg tacttggtgt tggtaccttt
    11341 gggggggcat caaaaattga tgccaactct ggaacgattt tagcgacagc tttctcagta
    11401 gcgctaggaa tgaacccaga gcaagcaatt gccgtaattg cggtaccagt agctagcttg
    11461 atgattcaat tagatatatt agcccgtttt gcgaatacgt attttgcgca tcgcattgat
    11521 aaattagttg aagatatgaa ctataaaggg atcgaacgta atttcttaat gggagccctc
    11581 tcttggtcac tttctcgtat gattcccgtt tttctagcat tagcatttgg aggaggacta
    11641 gtagaacaag tggtagtcgt gctaaatggt gatttgaagt ggttaggcga tggattatca
    11701 gtagctggag cagttttacc agctgtcggt tttgctattc tactacgtta cttgccagtt
    11761 aaaaagcatt tcccttacct aattttagga tttacattaa cggcattact aggaaccgtc
    11821 tttactaaca tgcaggtttt aggaacgggt gttgcaggag ctgtgaagaa tttcgaagga
    11881 atatttaatt cacttccaat gttagcaatt gcactagttg gattttcttt agcagccatc
    11941 agttacaaaa atggacagct gataccacat tcgccagccc ctaaagatgt tacatcgaat
    12001 gattcagatg aaggagagat tgaagatgac gaaatctaat tataagttaa cgaaggaaga
    12061 ctttaagcag atcaatcgca gaagcttgtt tactttccaa ttaggctgga actatgaacg
    12121 gatgcaagga tcaggctatc tttatacaat tctgccgcaa ttacgcaagc tttatggtga
    12181 taattctcct gaattaaaag aaatgatgaa aactcataca caattcttta atacatcaaa
    12241 cttcttcaat actatcgtta ccgggattga cttggctatt gaggaaaagg aaggtattga
    12301 tggtaaagac actgtctcag gtttaaaagc tgggttgatg gggccttttg cagcaattgg
    12361 tgattcgatt ttcgcagcgt taattccaac aatttttgga gccttagcag ccaatatggc
    12421 aattaatggc aacccaactg gaatttttat ttggattatc gcacaaatcg ctgttatgat
    12481 tttccgttgg aaacaattag aatttgctta tcgtgaagga atctctttag ttaccacgat
    12541 gcaacatcgc ttaactgcct tgacagatgc tgcaactttg ttaggggtct tcatggttgg
    12601 agcgttggtc gctaccatga ttaatgtaaa attgtcttgg gcaccgagta ttggtgatgt
    12661 gacgttaaac atgcaaaata acctggatat gattttacca cgattattac cagccggtat
    12721 cgttgcagca atttattgga tgcttggcaa aaagaatatg acttctacta aggcaatttt
    12781 catagtatta gtggtttgtg tagcattatc agctttaggc gtgatttcaa aataagtcag
    12841 gctgattcca attagtgatg aggagtggtt caatgggaaa acaattagtt ttaattagtc
    12901 atggactttt ctgtgaagaa ttgaaaaaaa gtacagaaat gataatgggg cctcaggaaa
    12961 gtattcacac agtaagcctt cttccttctg aatcagcgga agattttcgt gaaaagtttg
    13021 aaacagtaat tgccaattta gatgatttcg tagtattagc agatttaatg ggtggcactc
    13081 cgtgtaatgt agtttcaaaa ataattctcg agggtcaagt aattgactta tacgcaggca
    13141 tgaacatgcc aatggtgatt gacttcatta acagtcaatt aattggaaca gaactttcat
    13201 taatcgaatc tgctacaagt aacatttgtt atgtgaatgg acggattttc agtgatgacg
    13261 atgatgaaga tgagtaagat aaaatatatg agtgatgaag ccaaagaaat atttctctga
    13321 tcatactata gataaaagac atttcaaact tgtaatttga gtttggaatg tctttttatc
    13381 tagttttgtt caaaattttg gacaaaaaag agaccctttg tcaaatgatg ttggtaaaga
    13441 gaaattctag aatgattggt gaaagattga gaaaaaaatg ttcagcatca aggaataaga
    13501 aggaatttcc gaaatttgac aagaaacata aatacttttt tttggtgttg attattatct
    13561 acttggtgtg tctcaaattt tttataaatt tcagcgaatt tttgaagatg ccgcttttgt
    13621 ctcattctcc atttttttat tttatgatta acctcataaa aaagtgggac attaattaga
    13681 ttttttctga ttaatatccc agactcgtct acagactgag aacttggaag ggatacttcc
    13741 aggacttttt taaacggttg actcaatggc ttctttatac ttttcaaaaa gggggataat
    13801 ttgcgtgcgc ttttttatgt caagtttaat aaaaatattg tggacatgag ttttaaccgt
    13861 tcctaatgaa agaaagagct ggtcagcaat ttcctgattg gttcgatgtg acagtagcaa
    13921 tataaaaatt tccgtttctc gttgagtaaa ctggtgatcc tgacaaaagt cttgaatcaa
    13981 gagagaggtg tcttgctttt gtgttttttc tttctgaggt tgatattttt tgataaagaa
    14041 gtgcagtaat aaaacacata taacaatcga aaaaatatcc tctgagacac tacgattata
    14101 aatttttgtc actaaggagc tatattgatc gacattgaaa atcacgaaac tatcttcaat
    14161 aatgatcaaa atactaaaga agagattgat gaacatcaat tgctttaaat agtgtttgtt
    14221 cagcaaaggt acatcctttt ttgttcctcg ccaacaataa attcctaggt aaaacaggaa
    14281 gagttgattg cccagataat agagccagac tttaaaggct gaattatcca tcagtgggat
    14341 ggtaatcatc caaacagcca aagcaagcag taaagcataa tggagagggg aaagtttttc
    14401 tttttgtagt tcggcaagaa tagaaatgga aaaaaaggcg tttcccataa aaataatcgt
    14461 tttaactgct ggcgcactca taaatacttg atcataactt tgtgcaaagg aattgaggaa
    14521 ctccgtcata taaataatcg tgttatcaaa aatgaagaat gccaaataca aactgatcca
    14581 gagaaataag ttgttttttt cttttaaata agcataaata gcaaaagtca tcgtgattga
    14641 atatagaatg atcaatagaa tgttgtaaac aaaaatagct atcgtattga atcccctttc
    14701 atttttttcc ttacctcaaa tttcttattt taattataac accgattttt ttatctttac
    14761 ttttaattcc tataaacttc cttctaaact tttgtaaacc cttggaaaat aaatggttta
    14821 tatccaaaaa taaaccagcc tgtagcacgt atagatcggc aatgataacg tttacaacta
    14881 ataacttatc gtaaactctc actttttttc ctaaagtgaa gatgcaaagc aaacaaatta
    14941 cttaggaggt tccattatgg gaaaaaaaga ttttattaca actgaaaatt acacgaagga
    15001 agagattcaa caaattgttg acctgtcatt gaaaatcaaa gcggccatca agaatggttt
    15061 ttatcctcct ttgctaaaga acaaagtgtt agggatgatt ttccaacagt cttctaccag
    15121 aacgcgggtt tcgtttgaaa cagcaatgac acaattaggg ggacacgcag aatatctagc
    15181 accagggcaa attcaattgg gtggtcatga aacgatagaa gacacgagcc gagttctttc
    15241 tcgtttagtt gatattttga tggctcgtgt ggaacgtcat catagtgtgt atgacttagc
    15301 aacgaatgcc acgatcccag ttattaacgg tatgtctgac ttcaaccacc caacgcaaga
    15361 aattggcgat ctttgcacca tgatcgaaca tcttccagca ggcaaaaaaa ttgaagactg
    15421 caaggtttcc tttattggag atgccacaca agtctgtttc tctttaggat tgattgcaac
    15481 aaaaatggga atggaattcg ttcaatttgg accagaaggg ttccagttaa acgaagacca
    15541 tcgcgcacga ctagacgcaa tttgcaaaga ttctggtggc tcctatacga tatccgatca
    15601 aatcgatgtt atcgaaggtt cggattttgt ttatacagat gtttggtatg gactgtatga
    15661 agctgaacta tcagaagagg aacgcatgcg tattttttat cctaaatacc aagtgaatac
    15721 ggacttgata aaacgtgctg ggaaacaagc gaaatttatg cactgcttgc ctgccactcg
    15781 tggggaagaa gtcacagatg aagtcatgga tggaccgaac tcgatctgtt ttgatgaagc
    15841 agaaaatcgg ttgacttcga ttcgcggtct cttagtgtac ttgatgcgag attatgcgga
    15901 aaaaaatcca atcgatgaga ccaaaaaagc agcggctaaa aaagacttgg aagctttgct
    15961 aggtgaaaca ttttagacgc aagagatgag gaaaggaaat aatctgcgta tcatgacgat
    16021 gcgtgaggat tgtctctgtt ctagaggagg ggctaacgat ctgaaaatcg attctttcta
    16081 gcagaatctt gatctcacac taatgtagct tttatgccgc actagaaatc atacgaggtg
    16141 agtccgcagg ttacaagaat cgttcaatcg ctggtccaac cttattccct tgtaccggaa
    16201 aggaagaaaa caatgggaga aaagaaaaaa tttagtttga tgagtgctat attatctgtt
    16261 atttgtgttg tatttgtggc agaagcggct gcaccggtag cagcaatcgg aaattcacaa
    16321 tttttttggt ggatcttttt attgattgca ttccttttgc cctatggatt gatctcgtca
    16381 gagttaggaa caacttatat cggtgatggc ggtatctatg attgggtgac acaagccttc
    16441 ggacatcgct ggggatctcg cgtttcttgg tattactgga ttaattatcc actgtggtta
    16501 gcttctctag cagtagtctg ccctgattta ttgatgacga tcacaggcag cgaattttca
    16561 acagctgctg ctatcatgat cgaactgatc tttatatgga tcattgtttg gatcagtttt
    16621 tatccggtca gtgacagtgt gtggattttg aatggagcag ctgttatcaa aatgctgctg
    16681 gcaatcttga ttggtggctt aggtctctat actgcgttta ctaagggtgt ggcgaatgag
    16741 tttactcttt cttccatgct gccaagtttc aatttaaata gtttgtcctt tatttcagta
    16801 atcattttta acttattagg attcgaagtg atttgtacat tcgcgaatga tatggaaaat
    16861 cctaaaaagc aaatccctca atcgatcatt gccggtggac tggtgattgc agctatttac
    16921 attttttctg cattcgggat cggtgtagca attcctacgg atcagattag tacaggcagt
    16981 ggattgatgg atagttttaa gttattgaca ggatcaacag gcggctggtt cattatttcg
    17041 atggcctttt tattcttact gacattgttt ggcaatatga tttcttggtc gttaggggtt
    17101 aacaatactg cttgttatgc agccgaaaat gacgatatgc cgaaagtatt tgccaaacgc
    17161 agcaaaaaaa acggcatgcc tactggtgcg gcgattatga atggtattgt tgcttctatc
    17221 attggtgcga tcatcgttgt tgctgtagta tcatttttct tgggacgttc aagaggttaa
    17281 tcgtcaaata tatcagcaat tttacctatt atcttaagga gcagcttttc gctgcttctt
    17341 tttttgtata gataaactgc ttctatcact gatcatcgat gttgtctaac tgatgtcgcc
    17401 gaatgcaagc attacctttg ccagattaaa aaata
//
LOCUS       DAIGOK010000058        17399 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.058, whole genome shotgun sequence.
ACCESSION   DAIGOK010000058 DAIGOK010000000
VERSION     DAIGOK010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.058"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(119..664)
                     /locus_tag="NOA01_001520"
     CDS             complement(119..664)
                     /locus_tag="NOA01_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCI0632716.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(676..1368)
                     /locus_tag="NOA01_001521"
     CDS             complement(676..1368)
                     /locus_tag="NOA01_001521"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCI0632717.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1387..1671)
                     /locus_tag="NOA01_001522"
     CDS             complement(1387..1671)
                     /locus_tag="NOA01_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632718.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1684..2247)
                     /locus_tag="NOA01_001523"
     CDS             complement(1684..2247)
                     /locus_tag="NOA01_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCI0632719.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2475..3137
                     /locus_tag="NOA01_001524"
     CDS             2475..3137
                     /locus_tag="NOA01_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HCI0632720.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3261..4457
                     /locus_tag="NOA01_001525"
     CDS             3261..4457
                     /locus_tag="NOA01_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HCI0632721.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4558..5934)
                     /locus_tag="NOA01_001526"
     CDS             complement(4558..5934)
                     /locus_tag="NOA01_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCI0632722.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5971..6837)
                     /locus_tag="NOA01_001527"
     CDS             complement(5971..6837)
                     /locus_tag="NOA01_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HCI0632723.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6926..7951)
                     /locus_tag="NOA01_001528"
     CDS             complement(6926..7951)
                     /locus_tag="NOA01_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCI0632724.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7969..8643)
                     /locus_tag="NOA01_001529"
     CDS             complement(7969..8643)
                     /locus_tag="NOA01_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCI0632725.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIKGYIETKVG
                     QGYIVP"
     gene            complement(8827..9720)
                     /locus_tag="NOA01_001530"
     CDS             complement(8827..9720)
                     /locus_tag="NOA01_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCI0632726.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9794..11491)
                     /locus_tag="NOA01_001531"
     CDS             complement(9794..11491)
                     /locus_tag="NOA01_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HCI0632727.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11484..12797)
                     /locus_tag="NOA01_001532"
     CDS             complement(11484..12797)
                     /locus_tag="NOA01_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCI0632728.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12797..16834)
                     /locus_tag="NOA01_001533"
     CDS             complement(12797..16834)
                     /locus_tag="NOA01_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCI0632729.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FHHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16847..17305)
                     /locus_tag="NOA01_001534"
     CDS             complement(16847..17305)
                     /locus_tag="NOA01_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCI0632730.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
BASE COUNT         4883 a         3900 c         3022 g         5594 t
ORIGIN      
        1 gaaaaagaat aagttttttt aaaataacgt ataaaaaaga gcataaaaca aagtcgtttg
       61 ttttatgctc ttcttttatt attttgtgct ttttatttta tcaaatcact ttgttttttt
      121 ataaaatttc tgcgcctagt gtggactcaa aatgtctcaa tgcccattca tgtccaacgg
      181 gatcgaaact tgccacggaa tctttgaaaa tatggagttt gtatcctaga ttatacgcat
      241 cgactgctgt atgcaacaca cagatatctg tacatacgcc ggtcaaataa atatctgtga
      301 tctggcgttc tcgcaaacgg atgtctagat ctgttccact aaaagcggaa taatgccgtt
      361 tgtctatcca ataaacattt tcctcttgct catagtcttg gtataaactt tttaaagagc
      421 cgtacaattc ccgtccaccc gttcctatca cattatgcgg aggaaataag cggttctccg
      481 gatgaaagga atcttttgga tcatgcgcat caatggcaaa aacgacaaaa tccttttgat
      541 caataaactt ctttgtatga gcagtcagct ttttttcaat tacttgtcct gctgtcccag
      601 tagtcagctt tccatcctct gcaacaaaat catatgtgta gtcaatcgag atcaatgcgt
      661 tcatcttagt cctccttata cgagatgttt gacaaattcg atcaccattt ctgctgatcg
      721 tttgcctgcg tcttcgataa attcgtcaaa gctttgtgtc gccgagtgat ctgctgtgtc
      781 gctcattgca cgtacgatca aaaatgggat attgaactga cgagctgttt gtccaacagc
      841 ggctccttcc atttcgcaag ctaatgcttc agggaaattc gttaagattt ctttgatttt
      901 atctggcgag tcgacaaatg tatcacctgt aacgatcaat ccttcttttg cgttcaaatt
      961 cgttgctttt gctgctttaa ccatttcact gcgcaaatat gtgcttgctt cataatatag
     1021 aggcattcct ggtaattgtc ctgggttgta gccaaatcct gttgcatctg catcaaaata
     1081 agctactttg tctgatataa cgatgtcacc tacttgtaag ccttcaccga ttccgccagc
     1141 tgaacctgta ttgatcacca tgttcacgcc gtattgttgg atcaacagac ttgtggtaat
     1201 agatgctaag acttttccta taccggaacg tacgacgatc acttcgtggt tacctaatga
     1261 acctgaaata aataaagcgc ctgcgcgttc ccatgataat ggttcgctta ggttctctct
     1321 caaaattttt acttcttctt ccattgcgcc gataataccg attttcatct ttttaattcc
     1381 ttctttctat ataaatgcga ttgctaaaaa aaccacacat aacattagga taacaataat
     1441 aagagatttc atcaaaaaag agttccattt ggtcgtcttt tcccgttcgc tgatccttgt
     1501 tttcgtgatt ggtttattct ttttgctttc tttacgataa aagctggcaa tctttttttc
     1561 ttcttgctga taagctgttt ctgcttttcg ctgttttttt aatgactcgc ttgcttgtgc
     1621 ttgttgtcgc ttgcgaagtt cgcttcgcgt aataagcggt cctttggcca taagccgatc
     1681 cctctacttt cctttggtca atagttccca gtattgaatc gcataaatcg tctttgcatc
     1741 acaaatcaac tgatctttca tcgcttgttg cgcctcttca agagttagat gatacaactc
     1801 cagtacttcg tcttcatctt gtgctaaagg attctcgacc ttctctaccc cttgtgcatg
     1861 ataaatatga aggacttcat ttgcaaaacc tggtgaaaga tacatcgaag taagatggct
     1921 cagcgacttc gcacgatagc cagtttcttc ttctaattct ctagctgctg tcatttcggg
     1981 attttgtcct tctcctggat cgatttttcc agctggaatc tccaaaatca ctttttctaa
     2041 aggttttcga aattgtttga caagaagcag acgatcttgt tcgtcaaaag caatgatccc
     2101 gacaccgcca ggatgaaata ccagttctct cttcgaagtt cctccatctg gcaaacgaac
     2161 ttcgtctaca gcaacatcaa taatttttcc gctatagatt tctttgcgac tgattgtttt
     2221 ttcttcaaat tgctcattgt tcagcatatc ttctcctgct ttctttgtaa tcataactgc
     2281 ttttttttca tttttcaatt tttctcaaaa actccttatt agtttaccat agataaacct
     2341 tagattttct ctaaatatag aaaatccttc tttagtccga tactctttct agtagtccgt
     2401 atgctaaaat aagtatgtaa tcgaataacg aaaagaattc aatactcaac gataaatgac
     2461 atggagggtt tattatgata aaacgaaatt ggccgtggct tctagcattg gtcttgctat
     2521 ttctctttgt ccgaaacaat agtttttctt ttgttctagg acttttattg ataggtgtag
     2581 ttttagtttt gtggggactc tttggttcgc gcaggaaaaa gggaaaacaa gaagaaattc
     2641 ctgcactgac tgacgaactt gaaactcact atgaaaaaag cggtatgaca gcgaatgaaa
     2701 tttcattttt tcgacaaaca atgaatcaaa caaaaaatga gatcaatcaa ttgcaaatga
     2761 atatgcagca gacagccaaa cttaaagcaa tcgatctccg ccatgatcca gtcaaagcag
     2821 cgaaagcttt attcaaagaa ttggtgaaag aaccaaaccg actgcatgaa gcaagtcagt
     2881 ttctttatac gcatctgccg aatctagttg atttgacaaa taagtataat gagatcaatg
     2941 accatgagat caaaaacaaa cagacctatg aaaaactaga agaaagcgcg cagatcatcg
     3001 accagctgtc agctttaatc gcacaagatt atcaaaaatt cgttgctgag gatctagatg
     3061 atatcgacgt agagatatct gttgcgaaac agagcttaaa gcgtgataat aaatatgaac
     3121 cagaacaaaa ggattaactc ttttgttcat aaaagaagac tcggaacatt cagatgatgt
     3181 ctggctgttt cgagtacttt ttctaaagta gagacattca tgtaagaatc gagaacagat
     3241 tcaacaagga ggaatttttt atggatcata aaccgactga aacaaaagat gttacaccag
     3301 ttaacgatac acttgaagat ttattgaaca acccgttttc aactccaaca gatacgctga
     3361 ccacttcaca acaagcggag attgatcagc tccaagagca acagacagct gctcgtttaa
     3421 ttgacaaact accggcggaa cgccaagaac aagctaaaca gctagcagca aaaatcgatg
     3481 ctaatgatgc acagtctgtc atcagctacg gatcggctgc ccaagcaaaa ttaagtgaat
     3541 tctcacaatc gatgttgaat catgtgcaag cacaagatat tggtcctgta ggagattcct
     3601 taactgaact gatgtatcgt ctccaagaag ccaatcctga cgaactgcgt gccggagaag
     3661 ggaacatctt ccagcgtgtc ttcgggaagg tcaaacaatc gatctatgaa gtgacagcaa
     3721 aatatcaaaa gattggtgca caaattgaca agatttcagt gaaattagat aaagaaaaag
     3781 acggtctgct aaaagataat ctgatgttag aacagcttta ccagaaaaac aaagactact
     3841 ttgatgcact gaatatttat atcgctgctg gagaattgaa aatggaagaa cttcaaacaa
     3901 cgatcattcc agaagcgatg aaaagagcag aagaaactgg cgatcagatg gatgttcaga
     3961 ttgccaatga ttacacgcaa ttcctagatc gtctggataa acgtacgcat gatcttcgct
     4021 tagctagaca gatcacgatc cagcaagcac cacaaatccg tttgatccaa aacacaaatc
     4081 aggccttggc tgaaaagatc caagcatcta tcgctacagc cattccgctg tggaaaaacc
     4141 aagtcgtgat cgcattgacc ttgcttcgtc aaaaagatgc agtaactgct caacgccaag
     4201 tctctgaaac aacaaacgac ttattgaaga aaaattcaga aatgctgaaa atctcagcca
     4261 tcgaaacagc gaaagaaaat gaacgtggaa tcgtggatat cgagaccctt caaaccactc
     4321 aaaatgattt gatcgaaaca atccaagaga cattgcgtat ccaaaaagaa ggaaaagaaa
     4381 aacgtcgtca tgcagaagta gagcttggac acatggaaga agatctgaaa caaaaactgt
     4441 tggatttaac ccaatagatc acctttcgct tgttactaaa acaaacgccc aacaacagtt
     4501 tattacaact gttgatgggc gtttttctta tctttcttct aacttctcca ttggagtcta
     4561 tttctcattc atcttgctgt gtccatagcc atagccaaaa taaatgatca gtccaataac
     4621 cattacgata ccgaacacga tccatgtcac agtttgcaga cgaagcatca atacaataca
     4681 agtcaagatg gagacgattg gcaatagcgg aacgaatggc actttgaatt ctccagcttt
     4741 tggtttgcca aacatttttc tcaacttcag taagcctaga ctaacaagca tcaaataggc
     4801 gagcgccatg atattcgtta gttccgctaa tacattcaat gggaaaaatc cggcaaagaa
     4861 tacagctgcc acaccagcgg cataagtcgc ttttttcggt gtacgatgct tttgatcgat
     4921 ttctgtcata aacttaggaa gtaagccatc cttgctgaca gcaaacagta aacgagcaag
     4981 tgaatacatc atcgagatcg ttaccgtcag tagcgttaaa attgccccta cagcgatcac
     5041 cgcgcccact gttccatgtc ctacatacct cattgcaaaa gccactgggt cattgactcc
     5101 tagttcagta tacggaacga ttcctgtcaa gatcagcgtc acgatgatgt aaaggacagt
     5161 agcaatcagt atcgaaccaa tgattcctct tggcacatct ttttgcgggt ttttgacttc
     5221 ctcggctgcc attgcaacgg catcaaatcc caagaaagca aaaaacacaa gagctgcacc
     5281 atctagcaca ccggaaaaac caaaaggcat gaatggctgc cagttatcag gtttaacgaa
     5341 aaatattcct acaatgataa acaacgcgat gataccaaat ttgacatata ccatggcatt
     5401 attcaaacgt aatgctttct tggcttcaag cgacacgatg taagtgacga atatcactac
     5461 gagcaccgca atcaagtcaa tgtatgtacc attttccgga ttgtacccgc cagttaatgc
     5521 ttgtggaagt tcggccccta aacttttcaa aaaaccttgc atatagccag accatccgga
     5581 agcaacagag gaaaccgcta gtaaaaattc cccgatcaac aaccaccctg tcagccaggc
     5641 agcaaattca ccaaatacaa catacagata ggcatatggc ccaccaatca ctggcactcg
     5701 cgaagcaaat tctgcgaaac ttagtccaga caaaatgaca acgattgcag ctaagacaaa
     5761 tgaaagtgac agtgctggac cagcattttg attggccgca acccctgtca ccacaaatat
     5821 tcccgtaccg atgatcgcac caattccaag catgatcaaa tcagacgttt ttagttcttt
     5881 tttcatctca ctcgtatgat tgagattgat ctctttctta cgaaacaaat ccattgaagt
     5941 tcctccccat tttaggttgg aaaacagcga ttaaactgtt ttcatatacc ctaaaaggat
     6001 accacctaaa atgactaaaa ggcaaccaac aataacataa atgagctctt ttttcgtttt
     6061 tctttcgcca aggatgaaaa tcccacctaa agtagagata atgatcccca tttgtgaaag
     6121 tgagaagcca acagctaaac caagtgattt gactgttaga agcatgcaga tgtttcctaa
     6181 gccccataat aatccacaga tcatgttttt ccacacaaac tgatcgactt taactttacg
     6241 gaaagcaaag aagcttgcgc ctaagatcat tccgatactt tgaggtaaga taattgacat
     6301 cgcatccaaa ttcgcccagt tgacgatgat ggtatataca ccataaccaa tcgtagagaa
     6361 gatcaatgct ctaaaaccct taccaaaatc aagcatttta ttatctgtat ctatttttcc
     6421 ttcgtcatct tgtcgggcag tcaaatacgc accgcttaca agaagagcta aagcaataat
     6481 accgtataca taatctttcg tttgtgtcca ttcatggaag aaaacggctc ccgcaatcgt
     6541 attcaaaatt aattgaaaac cagtagacaa tggtagccct acagaaacac ccatgtactt
     6601 cattccatgg aactgtccat tttgtccaac actccaagct aagccggata aaaatccgat
     6661 caagaaaatc caaggggtga ttacaggact aacaacgaaa aagacaacta aagaaaacag
     6721 aaatgcaccg atcgtcattc ctagtgtttg ttgattggca tcgccaccaa ttttaccact
     6781 tactaaacca atactacccc atgccaacat agggactaat gctattaaaa tttccataac
     6841 caatctcctt ttatttcaca tgataagaaa cttatcagcg aaaacgttaa caacacaagc
     6901 actttttttc gtttctaacg attttctaac gtttttcatt ataaaaattt aagattggga
     6961 aaaataaagt cattgttgtc ccttttcctt cagttgattc agccgtcaaa tggataccta
     7021 atttttcggc cagatttttt gctaaataaa gtcctaatcc tgttgaatgt gttttgctaa
     7081 gtcgaccatt tctcccagta aatcccttat caaagatccg tttcaagtct tccgctggga
     7141 taccgatccc tgaatcttta actgaaaggc tgactccttg cgcttccttt tctatgctca
     7201 caatgatgtt tccgtgatcc ggcgtatatt tgatggcatt actaagcaat tggttgaaga
     7261 taaaagcgat ccatttagca tcggtcaaaa cagtatgatc ctcaccaacg atctcgtagc
     7321 gaatattttt ttggataaaa taatttgcct gactgcgtat cacaggctga atgattcctt
     7381 ttagtccata ttcttggatg aggtagtccc gcgagaaact gtcgagtctt gcataataca
     7441 aaacttgttc tacgtattca tcgatttttg ataattcatt ttctagtaat gtatactttt
     7501 gatcgtcaat atcgaactcg atcgaacgaa ctagcaattt agaagcggaa agtggcactt
     7561 tgatttcatg gacccatgag tcaatataat ctttttggtc ttgttgatta ctgattgctg
     7621 attgcattac ttcttggtgt tcacggatca gctgattgat atattcttct accagttttt
     7681 cttcttctgt cctcccacca ctcaaataat tttgcaaagg ggaagttttt tgtatgtcgg
     7741 ctaattttcc ccaccatctt ctcttgctca agtaaccacc cagcagataa aaaatcagaa
     7801 agacgccctc aatcaaagct aaataagcaa ttgtgctcaa atcaactttg atgtttggcg
     7861 ccagccacat cacaaagatc gtcaatgcaa taaaaaacag ccatcccaag atcaatgtcc
     7921 actgatcttt caaatattta aaaaaattca tcttaaccgc tcatcctttc agggaacgat
     7981 atatccctgt cctacttttg tttcgatata tcctttgata cctgcctgtt cgatttttcg
     8041 gcgtaaccga ttgatgttga ctgtcaatgt attgtcatct acaaatcgtt cgtcatccca
     8101 cagtgctcgc agcaattttt cccgacttaa gatctttcca ttgtgcttca tcaaaataaa
     8161 taatagacga tattcatttt tgctcaaatc aatgatctct ccgttgattt ccatacttcc
     8221 gttgtcaaca ttcaacgtaa tgccattgtg gctcatcatt tcactagata attctgtgta
     8281 gttgtaagaa cggcgaagta atgcattgat ttttgcaatc agtacatcga tttggaaatg
     8341 ttttgtcaca aaatcatctg cccccatgtt catagccatg atcatatcca tattcgtatt
     8401 cctgctggag ataaaaatga tcggtacttt cgatacttca cgtatttttt gacaccaata
     8461 atagccatcc aaaacgggaa gattaatatc tagtaataca agctgtggtt cttctctttc
     8521 aaagtctgag aacacttgat tgaaatcggt cgttccaaag gtttcaaatt gccatttctc
     8581 aagttcctcc atgatcaatt gtcgaatgat ctcttcgtct tcgactacca tgatttttgc
     8641 cataaaaaat ccctccatct ttcttcttgc tactacaata tcaaagaagc ccttgtgaaa
     8701 caagttggct tacctggaaa atgtcatttt agcaaacgtt aaaaaaaact ggaaatatgc
     8761 gatttaatcg cgtttgtctc ttccaagcag cttaaattta ttatgttatt cttaataatt
     8821 ggtctattat ctgttatttt tctttcgttg tttttgtctt ttaaattttc gataacacca
     8881 ataaccgaaa agcgttagca ctagcaatat acctagaagg agtataagta atcgataaag
     8941 atggtatttt tttgtctgtt ctatctttga agagacttct tctgtaaagg gaactcgtct
     9001 tcctgtcact agtaatcggt gagtgttcac ggtgtaaggt gtacaagtga taagtgtgac
     9061 tagatcctgc ttttctctaa ttgctaatgt atttgttttt gttggaagga caacaatttt
     9121 ctcaacgact tcataagcga gcatccgacc atacacttca agatagaatc ggtcatcttg
     9181 ctccatttct tcaagatccg taaagagttt tttctcagca ataccgctat gggcggtaat
     9241 cacggaatgt gtactctctc ctccagttgg aaaagaggtg ccttgcagga ctgtcgctcc
     9301 tctatctaac aaaagatcat tcgtttcatc aaataaaggc aagctaacat tgattttcgg
     9361 aatatagatg gctccgatca tatgttgttc gtaatattct tttcctggat tttggacatt
     9421 tctaaccgct tgctcaaaag gatctttgac ttgtcccatt cctgggatta ctgatgtatg
     9481 agcaagcgtc tgatttttct tttcatgcac tgctaattgt tgctcatgtt tttcttcttt
     9541 atcgcgttca agctgctttt gataattctc gatccttctt tgatccaaat aattgctgag
     9601 agcatctgct aaaaatggat atgtaaaaac tgcagctccg gcaagaaaca tcactgccat
     9661 aaataattgt aataaaagac tgatttgggt ttgaggtcta ttacctattt tctttttcat
     9721 tttcttcacc ttcttattta aaaaaactgc agcaaccttt ataaaaccga ttgctgcagc
     9781 ttatttttgt tgcttatact tcagacttca ttttagattt acggtaccag ctgtacgctc
     9841 caatcatcaa acttattccg attagtaaga atgccaagag cccgttccca ccagtagcag
     9901 gaagtgaccc gcgtttcata ttcggtactc tttctggccc tggattttct actcctacga
     9961 tctgaatttc ttcattatag cttccataag agatgataaa ttcagtaaag gctgcctcac
    10021 ctgttggaag tacataacct tctggcgcca tggtctcttt catttgatag gttccataat
    10081 ctaacccttg aacctcaaag tatccatttc catctgcgac aaattctgtt gcttgtccgc
    10141 ttgttgccca ctcaattcta tctgctcctg attcataaac agctaaaggc gactcccctt
    10201 tataaaatat tgcgtattca gaacggtctt cattccacaa ttcgaatctt gcatctttaa
    10261 gcaattcact gcttgatgca tcatattttt caaactttct tccacctgtt gtgactttct
    10321 ctggcggctc tactccttct tcataatcaa attcatcatt tcgtccgatt tcaataaccg
    10381 ctgtgttgtt atggaaatca tccgcttcta gttctgtgga aaccttcaaa ttatacgtga
    10441 tcgttaaatc tcctccacgt gcatctgcta atctacctaa agtatctact ttggctactc
    10501 cattttcttc tgtcaaaatc gtcacgacaa acccgttgct tactacttct actatataat
    10561 cttgtccttt tgttaaaatc gtctcgccgg ctcgcacttc tatttcctga tcataataag
    10621 ttagaccaac tgtaggcgta tctgtgattt tgtaattttt gataacagcg cttccgtttt
    10681 caaggacaga atcgatgaaa taaggaatag gtgctgtgat cgtgtaagaa gccgtttttc
    10741 ctctttctag atcaatataa gaaatcgttt cttgttctcc agcttcatta ataatcgtta
    10801 cttgtctaag gtcgctttcc tctacattca tctgtttatc tgcttctctg atttcgtttt
    10861 tcggatacaa atgcacatct gtattgtatg tgtctttcca tgtagaacca tcccacacgc
    10921 catccaccat atcaggcatc atcactggca tcataacaac cattggagac gcgattctgg
    10981 aaatatgagc tggcgaataa gtttctaaga aaagatagac tttatctcta gaagaaggtg
    11041 cttcttccgt cacttggacg gtagagaata ccgcttctcc agcttcattc gttgtttgtt
    11101 tgtcaatact tgtcagtcga tattttaaaa accaactttc tgtgtgccgt ttttgaatcc
    11161 attcaatcgc ttcggcagaa ttaagaccgt cttctcgttc cggtgttaaa ggattatctc
    11221 gaatcaaatc gtaatatact tccgttacgt caaacaaatc aaaatcgact cctggaatat
    11281 tttcttcacc aaaatccgta gaaaccaatc cgctattcgg tcgttcttcc ggaacagatg
    11341 agaaaccttt tttgtgtaat gtaatcgtca cttcacttgg tttttgtgcg acttcttcgg
    11401 ctttgactag ttttccgttc ccgaaaatcc ctattcccgc agtaacgagt aacagtacac
    11461 tcattcccgt gacaatcatt tttttattca tttttttctc cccgttcttt ttttattaat
    11521 aaaacagcac ctaacaataa aagaattcct agtaaagaaa tcaacgttct tgcttcattc
    11581 gtttgcggga atacggacca ttttcctgat tgacctggat tgcttggacc actcggctct
    11641 ttcggtttgt ccgttgtttc ttttacggtc tcgttataaa cacgtggtgt tgcggattca
    11701 taggcagaga ctggaacttt cccggattct aattcagcca tctcctgtcc gttaactact
    11761 acatattgtg gttggccttt tgcatccacc caaaattcag ggatgatgac ctcgattctc
    11821 ttacttgctt catccatctc gtaaccttcc acacctttta attcttcaaa ataataggtg
    11881 ccagaaggca agaaacgttg tcctgtcgta acaaggccct ctttatccga aatgaatttg
    11941 cttacatttt tgtcattcaa aggatcattg tctgctggtt tgatccattg atttttcaag
    12001 tcattagcag gagccatatc taaataatat ttcccccctt ctatcatctg atatagagca
    12061 aaaacagcac cttctaatgg ttttcctttt tcagttgttc ctctttgacg tccatacttg
    12121 aagaagtatg gatctcttag ataaccgaca tttttaggat agatatgaat ctcttgcagt
    12181 tcctcggttg attctgtcgg gtgataaatt ggcaaaatag cagcaattgg gatagctgtc
    12241 tgatcgatat caacatccaa tccaacttct ggatcaatcg attcttcgaa aatcaaataa
    12301 actgcatccc gatcttggtt tttcttaggt gctttgatac gtgcaatccc gtctcctaat
    12361 ccgccaaccg tatctttccc agttgttacc gaagtcccta tttggacaag cctttcttct
    12421 gtgattagct gacggatatt gtttctgttc gtttcagcga tttgctggac gaattcttta
    12481 ggagtcatgt tttcagcagc ttttttttca tgataataat cggtcatgtc gtaaatggca
    12541 aatgtcgctc catttagtgg caccgtctgt gaaatgatat ctgctgtttc ccctgcttcc
    12601 tcagagatag ccaagccatc attttcatgg tattcaaatt gatcgttata atcgatatct
    12661 cgaatcatcc gtttatgcaa cacgaattcc acgacatctt cccctgattc ttctgccgac
    12721 atcgcatgtg gaagaaggac tgctgcaagc ggtagtaaaa caagtaattg aagcatcaaa
    12781 cacaacagct ttttcattac cttgctccct cctttttaga ttgtctgaca aacaaaccat
    12841 aggctgctcc tgcaagcgcc atgaagaatg cagcaagcag gatatagaat aatcgtccgg
    12901 atcctcctgt agcaggcaac ggattttcag gatcgttccc gacactaagc tgtaatctga
    12961 ttcgatttgt ctccaaaagt tcatattcta actcttgctg tagatcaggc gtctctgctt
    13021 cttcgtttgg ataatacgaa atctcaactt ttagtgtacc cgcttctttt cctggaacta
    13081 cccgatcatt ttctatgtca ggttcttctc gataactaat ttctaaaata aagtaacctg
    13141 gaagttttct atagctgtca ggtgacttca cttccttgat ccgatagacc ccttccaaca
    13201 agtctgaagc attgaatagg gaagaatcgt ttggatctgg tgccaagcct ccctcgatga
    13261 tttcatactc tcctgcacta ttttgtttct caattgaaaa ttctgcgcct tcaagacgtc
    13321 ggctattcat actgtcaatt ttcaaaagtt ccaaatcgat tggcgctagt tcatcttcta
    13381 aggttacttg tagtacacca tcgatgattt catagccttc cggcaaggga gcagtttcgt
    13441 cttcgatgac cgtatcttca atgataaact taccttcttc attcagtgca aactggaaca
    13501 cttgatcaga tagccgatac ccatttggtg cgacagattc cgagacctca tattcaccaa
    13561 tagacagatg cagaggttgg tcgtaattta aatcgacata cttttctact cctacggcac
    13621 gagaatagaa gtatccgatc ccaggcaaac gtttcgattc ttctggttct aacggattac
    13681 cagtgtatgc tcgatacagc gggaaggaag aatcccccac tttcctgata tcgaaacctg
    13741 caccatctaa tgtatggcct tcatggtcta attttctaac tcggatctcc cgataatcaa
    13801 acgtattgta agcagtgaat gctacccata aacgatcctc ttcatctgtt gtatacgtga
    13861 gatattgcca attttcttca cctgtttgct tgagttggat agtccctata gcattatcgt
    13921 aaagtcgcaa gatccatgtg tcattttcat caggttcttt gaaaccttct gggttctggc
    13981 tctcaatgat tttgtattct gcatagttgg tagtttcttc actatcctta taaacaaatg
    14041 aatctggctc tccgattgga tgaataactt tctcaccatc cacgattaca tatttcccgt
    14101 cttcatctac tgaatagaac gtgagtctcc ctttctcttc ctctacttcg ttcacaccag
    14161 aaagcccttt cgctactgga tatccgattt ccagtccgtt accatctgtt tcataaagtt
    14221 caaactctgc gccattgatt ggcatattac tgccttcaat tagtttattg atattcatgt
    14281 ggattggctt gaattcattt cgcatttcca gtttgacata caactcatca tactctgtgc
    14341 cttctatgtt aggctcttgt acaacagaaa gcttgtgata ttctccttca taataggaat
    14401 tcggatcttt accttccggt ccgccgactc tcgaaccaga tgtatattca tctacaagct
    14461 cgtagtgacc taacatctca tcttcgttgg ctgcatattt tttgatttgg aattgatacg
    14521 tctcatctac taactgataa cgtggtggtc cagcgatctc tctgatccaa aatatgtcgc
    14581 tgactgctcc agtttcatct cctaaaatcg gcatcttcgt ttcaaagatc gctattccgt
    14641 ccgtggtagt tttacttgtt tcgattggtt cggcttcccc actctctgga ttttggtcat
    14701 ataatgcaaa ttcgactcca gccaacggct cgccattttg acccttcttg ctgacttgga
    14761 aattacggcc tttatgttcg taacgaatgg aagaagatgg gaaatacatc cgtccagtag
    14821 tgatatcatt ctcataatcc tccaggttct gaatataggc catattgttc actgtgcaaa
    14881 aatttccgtc ttgagctccc gcatcaagtt ttacttggta cttgatcctg accatttccc
    14941 cttcacctaa agaaatcgga gaaaatttat aagcttggtt ttcccgtccg acatacgttg
    15001 tcggagtcgc cactacttct ggtggggctt gtccatgacg ttcttcatcc catatttctg
    15061 gctcttggta cttcatgccg ttttctcctg gatctagatg gtaaaactga atatctgccg
    15121 atctgccagc tccaaatggt gagacattaa aaccagtttc atcataaagt gtggcttgct
    15181 ggatcgttct cttgaaggta gaagagatat cctccaaggc tgtaacaatg tcattcttgt
    15241 tgttcgcatt gtaatatcgc ggtgttacgc catcttcttc taaatcagca atatttttca
    15301 atacatttcg tccaatagca tctagtcgtt gtctcccgct aacgtttcca tcgattccta
    15361 aaccaatgga gaagatactt ggcgggttct cttgatactt gtttttcagc caatgatgat
    15421 aagcaactgt gttgatcgca gatgactttt cttgcgaagt aggactgcca ttcgtattgt
    15481 tattagaaga accatcacca acccagtgag tcgctttatt atctccttct tttacctctc
    15541 cataacgata gtgtacattt acttcatctt ctggacggtt aaagtcagat gcataggtaa
    15601 ctggatgagt aaaccctgca ttagaagtat tcccagaaaa ctcctcaaaa tttctgaaca
    15661 aaacgtcatt atcttcagca atcttatctt ccatcacagt ccagttgctc caagctccat
    15721 tagtgctcgt acgataactt gactgaatcg gtgcataact gaaggtcggt gtcccatctc
    15781 ccacaacgat taggattttt tctggatttc tattttctcc gccattgtct ttatacaatg
    15841 tttcatagcc atttttaagc cctagtgtaa gcggagttcc tccgattggt gtacgattta
    15901 atgctgtatt ctcacttccc gtcaaacgat tgatatcatt tgtcaatgga aagatgtccg
    15961 aactgatttg ctcatggtta ttgatagctg tactattatg gtgaaaattc accataccga
    16021 ttcgaatatc cagactcggg tcagagagca gatctttact catttcgttt actgcctcaa
    16081 tcaacgcagc atttttagtt tgactattgg tcccagcggt taattcactc atactcgctg
    16141 acttatctag aacaaaaacg atatcgattg gactgatttc ctttttctcg cccccgatga
    16201 catctagata gacattgtaa agggttgttg catcttcagg atgtagatca gatgctgtgc
    16261 tgcttggcag aacaggttct acccattttt tgatgtacgc cccgttataa tccaagtaac
    16321 tgttttcaaa attccgttca tttccccaaa gctgaacggt ttgagctgga acgtttctat
    16381 cttcttcgcc tgcagttgat ccattattag tagcatagtc gtaatttcgc cagttcgctc
    16441 ccccgtttgt ataatcacta tcatcgatca agttctctcc gtcatatggc gagcctttgt
    16501 ctccttgata gcgttcattg ctttgtgtcc aatttcctaa cacttttgta tagtcattcg
    16561 ttgtaaactt cgggtagcga tgctgttcgt tttcaacttc tccgctagga tagatatatt
    16621 cataggggtc ttgcgtagct gttggcccaa gacgctccaa aataccgttg aataactggc
    16681 gttgaatgcg ctggagtgct tgaccgctgg gtacgattgg ttcgtccagc ttttccatct
    16741 cattttcttc gctacgttga atttcagcgg aatcttctgt atcagtttca ttttcttgta
    16801 attcttggtt atctattctt tcatcaacca ccacttgaag aggtagttac ccttcgttca
    16861 catcggggat cttttctgtt tcaaaaatca atgtgccggt ttctgcttct tttgaataaa
    16921 cttttccgac ataccattct tgaccatcga tcgtctcgac ttttaggtct tctccatctt
    16981 catcatagga ggatagacct gttccacgaa cgttttttac ggtaccaatc ccggcggctg
    17041 ccgcatcgat tgctaaacgt attctacctt cattggcact ttcctttttt tcaaaagcca
    17101 gatgccattc aagggtttct tctttttcaa tatacttccc ttcaattttt gcatagtcct
    17161 catctaagaa tacctcatcc tcctttgttt ctgttgactc agcagctaca tccagggtcg
    17221 tcatatgata gactgactgg aaatatggtc caaatagcaa actgacagtt actaactgca
    17281 aaagcttcaa ttttaaccct ttcacatcct tatactcctt tcaaaaatat ttgtcttctt
    17341 tcacaacaat caacaaaaca atgcccatct aattataatt taatttaatt ttaatttta
//
LOCUS       DAIGOK010000059        17128 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.059, whole genome shotgun sequence.
ACCESSION   DAIGOK010000059 DAIGOK010000000
VERSION     DAIGOK010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17128
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.059"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            332..946
                     /gene="gmk"
                     /locus_tag="NOA01_001535"
     CDS             332..946
                     /gene="gmk"
                     /locus_tag="NOA01_001535"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCI0632731.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            951..1256
                     /locus_tag="NOA01_001536"
     CDS             951..1256
                     /locus_tag="NOA01_001536"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCI0632732.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            1509..3917
                     /gene="priA"
                     /locus_tag="NOA01_001537"
     CDS             1509..3917
                     /gene="priA"
                     /locus_tag="NOA01_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCI0632733.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            3932..4423
                     /gene="def"
                     /locus_tag="NOA01_001538"
     CDS             3932..4423
                     /gene="def"
                     /locus_tag="NOA01_001538"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCI0632734.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            4416..5354
                     /locus_tag="NOA01_001539"
     CDS             4416..5354
                     /locus_tag="NOA01_001539"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCI0632735.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            5354..6712
                     /gene="rsmB"
                     /locus_tag="NOA01_001540"
     CDS             5354..6712
                     /gene="rsmB"
                     /locus_tag="NOA01_001540"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCI0632736.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            6725..7465
                     /locus_tag="NOA01_001541"
     CDS             6725..7465
                     /locus_tag="NOA01_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HCI0632737.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            7462..9531
                     /gene="pknB"
                     /locus_tag="NOA01_001542"
     CDS             7462..9531
                     /gene="pknB"
                     /locus_tag="NOA01_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HCI0632738.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSREESSSSTSSSA
                     STSESSK"
     gene            9696..10595
                     /gene="rsgA"
                     /locus_tag="NOA01_001543"
     CDS             9696..10595
                     /gene="rsgA"
                     /locus_tag="NOA01_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HCI0632739.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            10608..11258
                     /locus_tag="NOA01_001544"
     CDS             10608..11258
                     /locus_tag="NOA01_001544"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCI0632740.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            11259..11897
                     /locus_tag="NOA01_001545"
     CDS             11259..11897
                     /locus_tag="NOA01_001545"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HCI0632741.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            12052..12906
                     /gene="mreC"
                     /locus_tag="NOA01_001546"
     CDS             12052..12906
                     /gene="mreC"
                     /locus_tag="NOA01_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCI0632742.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            12910..13419
                     /gene="mreD"
                     /locus_tag="NOA01_001547"
     CDS             12910..13419
                     /gene="mreD"
                     /locus_tag="NOA01_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCI0632743.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            13674..15248
                     /locus_tag="NOA01_001548"
     CDS             13674..15248
                     /locus_tag="NOA01_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCI0632744.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(15316..17115)
                     /locus_tag="NOA01_001549"
     CDS             complement(15316..17115)
                     /locus_tag="NOA01_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HCI0632745.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
BASE COUNT         5650 a         3229 c         3666 g         4583 t
ORIGIN      
        1 tattaagcca aatttatgca aaaatgtgag aaactatttt cacaaaattt ttcttcatac
       61 tcgaaactaa tcggcttctt cacgacctct tatacacggc gttcaaaagc ttgcatctgc
      121 gataataacc ccaaccaaac gaaaaacatg agaagctgtt tatgtttggc tgttcttatt
      181 acttgatgca ggacagcttt tttacgacct cttggtacag ggtagctttt ttaccaattt
      241 ctagctataa ttaaagataa ccttgaaagt tgcagaaaaa aagtgcaaaa taaacgataa
      301 gtacaaaaag actagttgaa aggacgtcgt catgtcagag cgtggattat taatcgtgtt
      361 gtctggtcca tccggtgtag gaaaaggcac agtacgaaaa gcaatttttg agagtgagga
      421 taatgatttt cagtattcga tctcaatgac tacccgtaaa atgcgtgaag gggaggtcga
      481 aggcgttgat tatttcttcc gaagcaaaga agagtttgaa tcaatgatcg aagctggaga
      541 gatgctggaa tacgcagaat atgtcgggaa ttattatgga actcctttgt catatatcaa
      601 caagacattg gatgaaggaa aagatgtttt cttggagatt gaagtccaag gagcaaaaca
      661 agtcaaagaa aaagtgcccg atggtgtatt catctttttg actccgccag atttagcgga
      721 attaaaatca cggatcgtag gccgaggaac agatgcagat gaagtaatcg aagaacgaat
      781 gaaagttgca aaagaagaaa tcgaaatgat ggctctttac gactatgctg ttgtaaatga
      841 tgaagtgccg ctagcagttg aacgaatcaa aaacattatt gccagtgaac attttcgagt
      901 agatcgcgta atcggtaaat atattaaaat gttaaaggag atgtagtctt atgatgttaa
      961 aaccttcaat tgactcacta ttagatcgtg taaattcaaa atattctcta gtgattttag
     1021 caagtaaacg cgcgcatgaa ttggatgcaa gtgcacagcc tactctagat tcttttgatt
     1081 cagtaaaaag cgtcggtcaa gctttagaag aaatcgatgc aggaaatgta gtcaatgatc
     1141 cacatcctga attaaaacgt gaacgtttga agatggaaga agaggaacgt caagcacaaa
     1201 aagagcgtga acaacacgaa ttggaatcac gtatccgtga agagcaaaaa atgtaataaa
     1261 gaaaaataga gcgaacatct aaaaagaaaa gcaaacccag ctgtttcctt ttcaaacatt
     1321 cgccacttat tgtctggtag aaaaggctgt gtctgaactt tttgacacag ttcttttttt
     1381 tcttatctgc tgttaatttt ttgttaggat ttggcagaag ataagacaaa tccatcgatc
     1441 tctagtaaaa taggcaagga actttttgtc gcttgataag aaaagacaaa aaaaggagga
     1501 aacgaaccat gacattaaca gctgaagtca ttgtggacgt acctactatg cagaccgatc
     1561 agccattcac ttatttggtt ccctctgaaa tggaaacagc tttgcaagtc gggatgcgag
     1621 tagaagtccc ttttggtaat ggcaatcgtc atgtccaagg atttgttatg gctatccaaa
     1681 aatcagaaga atcagctaat ccatctttga aggcagttat tcgtttgcta gaccttgctc
     1741 cggtggtcaa tgaagaatta ctttcattag ctgattatat gaaaaaaatt acttacgcat
     1801 tcaaaatcac ttgtctacaa acgatgcttc cgagtgtgat gaaagcagaa tatgataaat
     1861 tgatctatcc tttggcagac acaccagaag ttcaggtatt gtttgatcag cgagaagtga
     1921 tttcctggaa ggaagcagaa gaagaaggca gcctttctca attgattcgc tggcgccaag
     1981 agcaattagt agatatcaaa tatgaagtcc atacacgaaa taaagtaaaa acgattcgtt
     2041 tggtccgttc tttgttaact gaaaagcaaa tcgaagaaga atgggcaaaa ttacggcaaa
     2101 atgccaagaa acaaaaagaa cttcttttgt gtttatctga actgagccaa gaagaaccaa
     2161 ttgcttattt caaaaataaa ggaatcagta ctgctgtctt gaatcaagga aaagaaaagg
     2221 gctggctgga gtttatagaa tcagaacgtt atcgcgaccc ttataaagac cgagtatttg
     2281 accaaacgac ggcattggaa ctgaatgcag aacaaaaaaa tgctgtcgaa caaatcatca
     2341 cagcaggtca gcagcaaaaa gatcaggtct ttttgctgga gggaatcact ggcagcggga
     2401 aaacagaagt atatcttcag gcaatcgctg atgttttgtc agaaaataaa acggctatta
     2461 tgttagtgcc tgaaatcgca ttgactccgc aaatggtcga gcgattcaaa ggacgcttcg
     2521 gagaatcagt tgcggtctta catagcggac tttcccaagg tgaaaaatat gatgaatggc
     2581 gcaagattga gcgggaagaa gcgcaagtag tagtaggcgc acgatctgct atttttgccc
     2641 cgctgaaaaa tatcgggctg atcattatcg atgaagaaca tgaatcaagt tacaaacaag
     2701 atgagacacc gcgttatcat gcgagagatc tagcaatttg gcgcagtaaa tatcatcatt
     2761 gcccaatagt tctaggaagc gcgacgccct ctttagaatc aagagcacga gctcaaaaag
     2821 gcgtttatca gttattgcag ttaaaccacc gagccaaagc agctgcacag cttccagcta
     2881 ttgagatcgt agatatgcgt gaagagtttc agaatcatcg gacctctact ttttctgcta
     2941 atttgcagga aaaaatacaa aatcgattag acaaaaaaga acaaactgtc ctactgctca
     3001 atcgtcgagg atattcgtct tttgtgatgt gtagggactg tggatttgtt cttccatgtc
     3061 ctaattgcga tatctctctt acactgcata tggatacacg ttcaatgcgc tgccattatt
     3121 gcggacatga agaacctatt cccaatcgct gtcctaattg tggcggcaat aaaatccgct
     3181 actatggaac aggtactcaa aaagtagaag aggaactccg agaactttat ccgcaagcta
     3241 ggattttgcg tatggatgta gataccactc gaagaaaagg ggcacacgaa cagattttac
     3301 aaaaattcgg tgcaaaagaa gcagatatat tattagggac acaaatgatt gctaaaggtt
     3361 tggactttcc agaagtgaca ttagttgggg tgttgaatgc tgatacctcc ctgaatttac
     3421 cggattttcg ttctagtgag catacattcc aattgcttac ccaagtttca ggacgagcag
     3481 ggcgagcgga aaaagcaggc gaagtcgtga tccaaacatt caatcctcaa cattacgcca
     3541 tagaattagc taaaaaacaa aattatgagc agttttatca gcaggaaatg catgtacggc
     3601 atcgtggagg ctatcctcct tattacttta ctgtgaaaat aaccgccagc catcctgaag
     3661 aacaagtagc ggcaaagaaa atctttcaga ttgccaatca gctaaaagaa gtgttaagcc
     3721 ctcaaagctt gctattaggg ccaacgccca gcatgatttt gagagtgaaa aaccgatatt
     3781 attatcaact aatcatcaaa tataaacatg aaccgaatct tcctcatgtt ttggatgaaa
     3841 ttttgaatgg aagccagaaa gaacagcgac aaggtctttt tgtggcaatt gacaacgaac
     3901 cattaaactt tatttaagaa agggccaaat tatgcgctat cctatattga tccaccctaa
     3961 tgataaatta aaaagaactg ctcagccaat cgatgtaatc acggacgaga caatcgcttt
     4021 attagacaat ttatatgaga caatgattgc gaatgacgga atagggatcg ctgctcctca
     4081 agtaggacaa aacaagagaa ttgctgtgat cgaagtagat gaaggcgaga aatttgaact
     4141 gattaaccca gaaatcatcg aagcaaaagg tgaaagcctt gacgtagaag gttgtctcag
     4201 tattccgcat gtctatggaa cggtcaagcg agcagatgaa gtgaccgtgc gttattatga
     4261 tcgagacgga gaagagatag aagtgacggc ttttggctat ttagcccgtg ccttccagca
     4321 tgaaatcgat cacttagacg gtatcttgtt cattgagaaa atgatacagc agatcccgga
     4381 agaagaactt gaagaatata tggaggaaca tcttgatgac taagctagta tttatgggca
     4441 cacctgcttt ttctgtgccg attcttgaag ggctgataga atcaggttac gagatccagg
     4501 cagtggtaac acagcccgac cgtccagttg ggcggaaacg agtaattacg ccgacaccgg
     4561 taaaagaagc ggctctgaag cacggtatcc gtgtgctgca gccagagaaa atctccggtt
     4621 cgccagaaat ggaagaaatc attgaattgg tgcctgatgt gatcgtcact gcggcatttg
     4681 gccaattctt gcctgaaaag ttattgcaag taccaaaatt aggggcaatc aatgtccatg
     4741 cttctttact tcctaaatat cggggaggtg cgcctgttca ttatgccatc atgaatgggg
     4801 agaaagaaac tggtgtaacg atcatggaaa tgatcaaaaa aatggatgca ggcggaatct
     4861 atgcgcaaga aagcatacca atcactaaac aagatgatgt agggacgatg tttgaaaagc
     4921 ttagcctttt aggaagaaag ctattgctag aaacattgcc caatattttg gatggacaaa
     4981 aaccagtacc tcaagacgag tcagaagcaa ctttttcgcc aaacatcaca cgggaacaag
     5041 aagcaatcga ttggcataaa actgcagaag aaattgacaa ccaagtacgc ggaatgcgtc
     5101 cttggccaat tgcattcacg acctatgagg gcatacgctg gaaattatta tcggttgaag
     5161 tattggcaga aactacagac aaagcacctg gtacgatcat ccggaaagac aaaaaaaatc
     5221 tatggattgc ttgcggtaaa cagaccgtct tagccatcaa tgaattacag ccagctggta
     5281 aaggaaaaca ggccgtacat gatttcctta acggaagcgg tcagcaagtt cagattggtc
     5341 agcaggtgaa ataatgaaaa aaatccctaa aaaaatgaaa aaatcagtac gatatatggc
     5401 attactgaca ttagagcgag tgaacaaggg tggcgcctac tcgaatcttt tgttgaatga
     5461 gatgatcaaa caaagcgagc taaatcaaaa agatgtgggt ttatttacag aattgatcta
     5521 tggtacgatt agccatcagc gactattgga attttacgtt gcaccattga tcgctaaagc
     5581 aaaaaaagtg gatgactggg tcaagacgct tctttatctt tctgtatacc aattagaatt
     5641 tttagataaa gtccctgcac atgctatttt aaacgaggcg gtcgaaatcg caaaagtaaa
     5701 aggaaatcct gggacaggga aatttgtaaa tggtgtcttg aggaattatc agcgtcaagg
     5761 agcaccagat ctgcaaggca ttgctgatcc tatcgaaaga ctttctgtca aaatcagcct
     5821 gcctctatgg atgacgaaac gactggtgga tcaaatcgga tacgaagaaa ctgaaaagct
     5881 cggtttatcg ctatatcagc ctagccatgc tagtgcccga gtagatacac gccgactttc
     5941 cagagatcag gccatcactg ttttgcaaga agaagagatc acagctgaaa aaagccaagt
     6001 cagtccatac ggtatagtag cgaaaaaagg gcatttagct tctagctcat tgttccacga
     6061 tggcgtcatg acgatccaag atgaaagttc gatgcttgta gcgccgtcta tgcaaatcga
     6121 gcctgaacat catgtcttag atgcctgtgc agcacccgga ggtaagacga cgcatattgc
     6181 ttctttttta gatccgtcat gcggtggaga agtggtcgct ttagatgtac acgaacacaa
     6241 agtcaaactc attgaagata atgcagcacg tctaggtgtc tcttctgctg tctacgctca
     6301 aaagatggat gctagagaag tcagtaaaca gttcgaagca gagcagtttg atcggatttt
     6361 agtagacgcg ccttgttcag gtcttgggct gcttcggcga aaaccagata ttcgttataa
     6421 aaaacagccg gatgaattag cgaatttacc aaaaatccaa ttggaaattt tagaaagctg
     6481 cgcaccaact ttaaagtctt ccggtattct gacttatagc acttgtacga tcttaaaaga
     6541 agaaaaccaa gacgttgttt ctgctttctt ggaaagacac cctgattttg agaggattga
     6601 tgttttgacc aatcctatgc tacaatcatc aatcgaagag aaaatgttga ctttgtatcc
     6661 gcatcagttt tacacagatg gatttttcat ttgttgtttg cgcaaaaagt aatgaggtga
     6721 aaaaatgcag attgaatatc aatcagatgt aggaagacga cgtaacacaa atcaagacta
     6781 tgccagtgta tttaccagcc gttcaggtct caagcttgct gtattagcag acgggatggg
     6841 tggtcatcgt gccggagaca ttgccagtca gatggcggta acgaatctag gcgaagcttg
     6901 ggaaaaacaa gaagctgttg acgatgaaaa aatcgcccaa tggttcattc aatcgattca
     6961 agaagaaaat atgctgattt atcagcgtgg acaagaacag ccggaatata acggtatggg
     7021 aacaacaatc gtagcggccg ctctgtcaga tgactggttc acgattgcca atgttgggga
     7081 tagcagagct tatttgattc gtgaagataa aatcatgcaa ttgacggagg atcattcttt
     7141 agtcaatgaa ctagtcaaat caggagaaat cagcgaagaa atggcagtga accatccaag
     7201 gaaaaatatt ttaacacgtt ctgtcggcat gcctggaaca gtcgaagtag atgtttctac
     7261 tcatctttgg caactaaaag atcgactgct gttatgttca gatggcttga cgaacatgct
     7321 gtctgaagaa gaaataggaa cgatcatcaa tcaagaaggc ccattgtctc ataaagtaac
     7381 tgagctgatt gatcgtgcca atgaagcagg cggagcggat aatatcaccg ttcttcttat
     7441 cgaatataag gaggacagtg tatgatcgag ttgggcaaaa aattaaatgg ccgttatcaa
     7501 atcactggaa atatcggtag cggtggtatg gcaaatgtat ttttggcgca cgatttaatc
     7561 ttggatcgtg atgtagcagt taaagtctta cgatttgact ttcaaaatga ccagactgcg
     7621 attcgacgat ttcagagaga agctttagca gcaacagaac tcgttcatcc aaacattgtt
     7681 agtgtttacg atgttggtga agaagacaac atgcagtatc tggtgatgga atacgtcaaa
     7741 ggaatggact tgaaacgata tatccaaaca cattatcctg ttccttatga aacagcagta
     7801 aatattatgc agcagatcct ttctgctatt tctttagctc ataaccatca aatcatccac
     7861 cgagatttga aaccccaaaa cgtactgatc gataatgaag gtgttgtaaa aatcacggat
     7921 ttcgggattg ctatcgcttt atctgaaaca tcgatcacgc agacgaatac gatgctggga
     7981 tctgttcatt atctgtcccc tgagcaggcc agaggaagta tggcaacgaa gcagtcagat
     8041 atttatgctt tagggatcat cctttatgag atgttgacgg gtagcgtacc gtttgatggt
     8101 gaatctgctg taacaattgc tttaaaacat tttcaagatg atcttccatc tataaaagca
     8161 cttgatccaa atgtgccaca agcactggaa aacgtgattt tacgggcaac ggctaaagaa
     8221 cctgcagacc gttataagtc agccgaggaa atgtctgatg atctttctac agtattatca
     8281 ccagctcgtg caaacgaaga aaaatggcag cctcatgtga tggataatga aacaaaagtt
     8341 atcacacccc ttacggaaga tgcaccaatg cctgattcat tcaaatctat gcctctgcca
     8401 aaagacaagc cagaaaacga aaccttgcca gaggaggaag aagttctgcc tgatcctaaa
     8461 aagaaaggac agaaaaaatg gtggctgatc ctattagcta ttcttgctgt tttaggaatc
     8521 ggaacagctg tattctttgc ttctggcggg cgaggagaag ttgttgttcc tgatgtttct
     8581 gatctttctg aagcttctgc tagagaaaca ctcgcaaaag cgaaattaga agtagcaaca
     8641 aaaacagaag aaattgcaga tgacaaaatc gaagaaggta atgtagtgaa gacagacccg
     8701 gcagcgggaa cgacagtcaa gcaaaaacgc gaagtcactc tgtatattag tagcggcacg
     8761 aaaaaaatca aattagatga ttacagtggt aaaagttatg aagatgccga agcaagtttg
     8821 aaagaattag ggttttctgc tgacttgatt caaaaagaag aggaattcag ttcagaggta
     8881 gaaagcggaa tgatcatttc gcaatctcca tcagaagata cagaagtcga tcctaaaaca
     8941 gacacgatta ctttcacagt cagcaaagga ccagaaacag tgactttgag tagtcatgta
     9001 ggagaaaact acgaaactgt tcgtaattgg cttgttacac aaggattcaa tatcaataat
     9061 attagtgcta cttatgacta tagcgacact gtaccatcag gttcgatcat cagtcaggat
     9121 ccagcctcgg gtgaagttgt agcagaagaa acttacatca attttgtcgt aagccaagga
     9181 acagagccta aattgaatga tatctccggc tatacaaaaa gcgaagcaca aagttattta
     9241 gctagtgtcg gtgcagaata tatcgggcat gagacttccg aatattcaaa cacggtggat
     9301 aaagataaag tgattcgtac agtaccaggg gcaggaacga caattaccaa aggaatggtt
     9361 gtgaacgtaa tttactctaa aggaccagat ccagcgtctt ctagttctga aaaagactct
     9421 tcagacagtt ctagcagctc aagtagctcc agtagcacaa gcaactcttc aagagaagaa
     9481 tcttccagct ccacaagttc atcagctagc acctcagaaa gttcaaaata agtgctgaat
     9541 aaaatatatc agaagggaat gcgacattga tttgttgcat tctctttttt gtgtttctat
     9601 ctcttttgat caattttcaa gccatatact ttgagaagtt tgcatggaat aacacctact
     9661 ttcctcttcg tatggtaaaa taaacaaaaa cggaggtggc acttatcaaa ggacaaatca
     9721 gaaaagcaat cagcggtttt tactatgtat acgcaaacgg agagacctat caaacaagag
     9781 gaagaggaaa tttccggaat cggaaaatca cgccgcttgt cggagatcag gtgatctttg
     9841 agagtgaaaa tcaaacagac ggctatcttc tggaaatctt gccgagaaaa aatcaattag
     9901 ttcgcccacc agtcacaaat gttgatcttg gaatcattgt cacaagtatc gtagagcctg
     9961 cattttctta taatctactt gaccgctttc tagttacctt ggaatacgag tcgatcgaac
    10021 cgattatttt tttgacaaag accgacttgt cagacgaccc agaaaaaatc gaagagatca
    10081 agcaaaccta tactaaaata ggctatccgg tgattgtacc caatcatacg ggtgatacga
    10141 cagaactgat ccgttacttc ccagaacggc tatcggtctt tatgggtcag tctggagcag
    10201 gaaaatcgac gctactgaat caaatatcac cggaactgaa tttagcaaca ggagagattt
    10261 ctgatgcact tggaagagga cggcatacaa cacgtcatgt ggaactgatt cctatctatg
    10321 gcgggttagt agcagataca ccaggattta gctcgattga ctttttgaca attgaagcag
    10381 gagaactgcc gagacaattt cctgaatttg tagaagcttc tgtccattgc aaatttagag
    10441 agtgcatgca tgctaaagaa cctggctgcg aagtaaaaca tagagtagaa acaggtggta
    10501 tcgcacagac acgttacgac aattatttgc aattcttaca agaaatcgaa aaaagaaaac
    10561 caatgtatgc aaaaaaagaa ggtaaacgaa aataggagga aacaagaatg aagatagcac
    10621 catccatttt gagtgcagat tttgccaatt tacaaagaga cattgagtta gtggaaaaag
    10681 gaggcgcaga ttacatccac gtagatgtaa tggatggcca atttgtccca aatatcacat
    10741 tagggccaaa tattgttcag gcaatccgtc ctgttactaa attaccgtta gacgtccatc
    10801 tgatgatcgt tgatccagaa aactacattc cagcatttgc aaaagctgga gcagatatca
    10861 ttactgtcca cgtcgaagca actcctcata ttcatcgggc acttcaaatg acaaaaagct
    10921 taggagttaa agctggagta gttatcaatc caggaacacc agtctccatg atcaaacatg
    10981 ttttgccttt ggctgatcaa gtactagtca tgactgtcaa tcctgggttc ggcggacaaa
    11041 gctttattga agaaacagta gaaaaaatta cggaactcgc tgatctacgt agagagaaca
    11101 attggactta tgcaatcgaa gtagatggcg gaatcgttcc agaaacagca aaaatctgta
    11161 aagatgcagg ggcggatgtc ttcgttgcag gatcatatat ctatggagcc gaaaatccag
    11221 tcggacaaat cgaacgatta aaagaggcat tagactaaat gaaaatttta ttagctgctg
    11281 gaggtaatcc gcaaaactgg ccgcagtctc tgctgtccaa atacgatgcc tatgtcggga
    11341 tagaccgtgg tgcgctgtac ctgcttcaag caggctaccc agtagatctg gcagtgggcg
    11401 attttgattc attgtcgaac gaagagcaga aggaagtatt tgcaaaagcc aaagagtata
    11461 cccgttcccc tgcagaaaaa gacgatacag atacacagtt aggtatctta aaaacatttg
    11521 aacgttaccc gcaagcggaa gtcactttga ttggagctac gggcggaaga ttggatcatc
    11581 ttttagccaa tctctggtta ggtatggaac ctcgcttcaa accctttgcc catcagctta
    11641 ttatcagaga ttgtcaaaat tatttgtctt attatttgcc tggagagcat atagtaagac
    11701 agataccaga tatgaagtat ttagcttatt gctgcttgac tcccgtctcg aatcttacat
    11761 taaaaggcag caagtatgta ttggaaaata aagaagtaga atatccgaca tcttatgcaa
    11821 gtaacgaatt tctttcagat accgcagaat tttctttttc agatggtatg attgcagtta
    11881 ttcaaagcaa agattgatcc ataaacaaat aaaaaaattt gtctttcagg ctaaaaatga
    11941 cagaatctat tatttgctaa aaaaaagctt aagaggagca acttaccttt tgtttcacgg
    12001 catttagtga taaaattgaa cacaagagtc taattaattc gaggtgtctt tgtgaagaag
    12061 ttcaatccta ataaaaatat tattattacc ttggttattg ttatcatcgt ggtgacgata
    12121 ctaagtgtga ccgctgcgca gcgagcgaat gaaggaaagc ccaatttctt ccaatccatc
    12181 gttaatgaca gtgttgcaat ggttgataaa acaatctctg ccccagtacg gtgggtacga
    12241 aatggagtag attctgtcca taatcttttt tcaacttatg ccgaaaatga gcggctaaaa
    12301 gaaaaaatcg atagctatga tgacgtttca cagcaaaaca agaattacga gagagaaatc
    12361 gaagcgttga aaaaagaact tgagttgaat cagacgctga ctagttacga aaaagtaacg
    12421 gcaaatgtca ttactcgttc gcctgacagt tggcaggaca tgctgatcgt agacaagggt
    12481 tctaaagacg gtatcgaagc aaatatggca gtcatgtcgc aaaaaggatt gattggccga
    12541 gtgatcgagg tcaatacggc ttcgtctaaa atcgaattac tgtcatcctc taatgaaagc
    12601 tccaatcatt ttccagtacg ggtatcttcg gctaatggcg aagcgtttgg tttgcttaaa
    12661 aactatgatg aaaagctcca tgccttagtg gtgacccaat taactggtga tacggatatc
    12721 aaagaagggg atgttgtcca gacatccggt cttggaggga attctccagc taacttgccg
    12781 atcggtacgg ttattaaaac gaaaccagat agttatgggc tggatcggga agtttatgtg
    12841 aaaccttatg cagaaatgta tgacgtgtca gttgtgacga ttgtccaacg gctagcggag
    12901 gattagtcca tgttgaagaa agaaacaatg aagtactatc tgccaatcgt tttgttcttt
    12961 ttgatgttga tagatggtca tttaacaaga atgctagggg agtggtcgaa aggcacctat
    13021 atgtcaaatg cccactttct gatattggca ttattatgtt gcagtatggc gtttgaaaaa
    13081 cgttatttac tgattaccac gattgttctc ggggctatct atgacgctta ctatattggc
    13141 gttatcggta tctatgcagt agctctccct ttaattgtat ggttgatgta tgtaatgaaa
    13201 gacgttatcc atgtcaacat ctttactgaa tttttcagta tgatcatctt tgtcacgggt
    13261 tatgaattgt ttacgatggt ggtccagttg atttttaaat tagcagtagt aaataacacg
    13321 tattttatta caaggttttt aggacctaca ctgctgttga acatgattat atttgtatta
    13381 ttcatttttc cctttaagaa attattcagt gatgaataat ttcttttttt atttttaggt
    13441 cgaaagcgaa aaagaatcgt tgttatctta agaaatgcta gacttaacgc ggctttttca
    13501 tgatcatttt ctgaaacgaa cagttttgtc atttttaaaa gttgttttga aacatttttg
    13561 taacataact attatatcgt tgacacactg ctatggtaca atttttatat cgttaagaaa
    13621 gacttaaata tatttagata tttattaaat taagccagtc ggaggaatga agagtgaaaa
    13681 agagtttaat atcagcagta atggtatgtt caatgacctt gactgccgta gcatcaccga
    13741 tcgctgccgc ggcggacgat tttgattctc agatacaaca gcaagatcaa aagattgcag
    13801 acttgaaaaa tcaacaagca gatgcacaat cacaaatcga tgccttagaa agccaagttt
    13861 ctgaaatcaa cacacaagca caagacctat tagcaaaaca agatacattg cgtcaagaat
    13921 ctgcacaatt agtaaaagac attgcagatt tacaagaacg tattgaaaaa cgtgaagata
    13981 caatccaaaa acaagcacgc gaagcacaag taagcaacac aagctcaaac tacatcgatg
    14041 ctgtattgaa tgctgattca ttagcagatg ctattggacg cgttcaagca atgacaacga
    14101 tggtaaaagc aaacaacgac ttgatggaac aacaaaaaca agataaaaaa gctgtagaag
    14161 acaaaaaagc tgaaaacgat gctaaactaa aagaattagc tgaaaaccaa gctgctttgg
    14221 aatctcaaaa aggcgatctt ttatctaaac aagctgattt gaacgtactg aaaacttcat
    14281 tagctgctga acaagcaact gctgaagaca agaaagctga cttgaaccgt caaaaagcag
    14341 aagctgaagc agagcaagct cgtattcgtg aacaacaacg tctagcagaa caagctcgtc
    14401 aacaagctgc acaagaaaaa gctgaaaaag aagcacgcga acaagcagag gctgaagcac
    14461 aagctactca agcttcatct actgcacaaa gctcagcaac agaagaatca tcagctacac
    14521 aatcttcaat gactgaagaa tcatcatcag ctacacaatc ttcagcaaca gaagaatcaa
    14581 caacacctga atcttcaaca gaagaatcaa cagcgcctga atcttcagca acagaagaat
    14641 caacaacagc gcctgaatct tcagcaacag aagaatcaac aacagtgcct gaatcttcag
    14701 caacagaaga atcaacaaca gtgcctgaat cttcaacaac agaagaatca acaacacctg
    14761 cgcctacaac accatcaaca gatcaaagtg ttgatacagg aaacggcaca ggaagtagta
    14821 ctccggctcc aacgccaaca ccaacacctg aacaaccaaa acctgtaaca cctgctccag
    14881 ctccttctgg aagtgtgaat ggtgcagcaa tcgtagcaga agcttacaaa tatattggta
    14941 ctccatatgt ttggggcggt aaagatccaa gtggctttga ctgctcaggg ttcacacgct
    15001 atgtttacat gcaagtaact ggtcgtgaca tcggtggttg gacagttcct caagaatcag
    15061 ctggaacaaa aatttcagtt tctcaagcaa aagctggaga cttgttattc tgggggtcac
    15121 aaggcggaac ttaccacgta gcgattgctt taggcggagg acaatatatc catgctcctc
    15181 aaccaggcga aagcgtaaaa gttggttcag ttcaatggtt tgcccctgac tttgctgtca
    15241 gcatgtaaga atgattaatc aaaaagagtt agcccattct ggactaactc tttttttatt
    15301 gtgataaaat agaaatcaga taaattcttc tgtcaattcc atgaacgttt ggatatcttc
    15361 agtcaccatg tcgatacctg cttgccaaaa atctggttgg gtaagatcga cattcaaatg
    15421 tttcttcgca agatcttccg tcgtcattga ggcagtatcg cgtagtaaat ggatatattc
    15481 ttcttcaaaa tttgctccct tttggttggc gtaagcatag atgcccaaac taaagaggta
    15541 gccaaatgtg tatgggaagt tataaaacgg tacgtcatca ataaagaaat gcaattttgc
    15601 agcccagaaa tatggatggt aagtagctaa agctccttga tagccttctt tttgcgcctc
    15661 aaccatcatc tctgtgatct tatcttctga gactaatcct tcttgccgtg cttcatagaa
    15721 tcgattttca aaaatgaatc gggaatggat attcatgaac atagctaaag cattttgcag
    15781 ttttgtatct agcaaattga ttttttcttc ttttgttttt gcgactttta atgtagcatc
    15841 ggctacgatc agttctgcga atgtacttgc tgtttctgcg acattcatgg catattcgcg
    15901 atttaaagaa ggtaaatccc acatcgtgct gctatggaaa gcatgtccta gttcatgagc
    15961 aagtgtcgca acctcattaa cagaattact gtaggtcata aagatccttg attcttttgt
    16021 ttctggtaac tcagtacaat aaccaccagg acgtttccca ggacgatctt ctgcttcgat
    16081 ccagctcttt tcaaaagcag attgggcaaa tttagccatt tttggactga atttgttaaa
    16141 gttttcgata ataaatgctg ctgcttcgtc aaaactaaag gttttttctt tcaaatcgcc
    16201 taaaataata ggtgcatctt gatcttgcca ttccattttt tctttaccga aaagattagc
    16261 ttttctagtc aagtaattaa caaggggttg tttgtttttc tggattgtat cccacatgac
    16321 agacaatgtt tctttcttca accgattgta ctctaatggt ttttgtagaa aatctgtgac
    16381 accatgaagt tcatagtcag aaagtctaaa accatctaaa tgattcaaag tatccgtgaa
    16441 gaggggtgcc ttttctttcc aggcttcttc ccatgctgcg aaaagtgttt ctctgacttt
    16501 tggatctgga tcgcccatca tcttattgaa ggcttgtcca gcagataatt cgactacttg
    16561 tccatcttgt tcgaagggaa tcgaaatgct ggctacaatc gtatcataat gactgctcca
    16621 agcatttaga ccatctaaag aaagcgtatt tataatgttt tcttcagctt ctgataataa
    16681 ttgtgagcca tcacgacgaa tctcgtttaa acgaaaagca attgtttcaa acgaagattg
    16741 agaaagcatc tgattccaat cattgtcaga tatttctgca aatttctttg acaatacagt
    16801 ttcggctgat tgcagacgag gcagcattgc gtaaagcttg cctgataaga cctttgcatc
    16861 tgaatcattg acattggcag aaagcaatgc agtaatatag ctgctgcatt gggtaaaacc
    16921 attcgtaatc gtttcttgca attgaagaat agcgtttaac tgctctgcat tgtcagaact
    16981 aggtgaccat tttgttacgc ggtgataata ctcgttcatt tggtcttcca attgcttcat
    17041 ccgttggttc aaagcatcag aatgactgcc tccggaaaaa atagaatcta gattccaatt
    17101 taatgaataa ctcatgaaaa aactccct
//
LOCUS       DAIGOK010000060        17007 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.060, whole genome shotgun sequence.
ACCESSION   DAIGOK010000060 DAIGOK010000000
VERSION     DAIGOK010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.060"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(<1..126)
                     /locus_tag="NOA01_001550"
     CDS             complement(<1..126)
                     /locus_tag="NOA01_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HCI0632746.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
     gene            complement(230..541)
                     /locus_tag="NOA01_001551"
     CDS             complement(230..541)
                     /locus_tag="NOA01_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HCI0632747.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(543..740)
                     /locus_tag="NOA01_001552"
     CDS             complement(543..740)
                     /locus_tag="NOA01_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HCI0632748.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1134..2861)
                     /locus_tag="NOA01_001553"
     CDS             complement(1134..2861)
                     /locus_tag="NOA01_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HCI0632749.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2854..4047)
                     /locus_tag="NOA01_001554"
     CDS             complement(2854..4047)
                     /locus_tag="NOA01_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HCI0632750.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4234..4878)
                     /locus_tag="NOA01_001555"
     CDS             complement(4234..4878)
                     /locus_tag="NOA01_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCI0632751.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4972..5106)
                     /locus_tag="NOA01_001556"
     CDS             complement(4972..5106)
                     /locus_tag="NOA01_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HCI0632752.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5108..7240)
                     /gene="feoB"
                     /locus_tag="NOA01_001557"
     CDS             complement(5108..7240)
                     /gene="feoB"
                     /locus_tag="NOA01_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HCI0632753.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7237..7710)
                     /locus_tag="NOA01_001558"
     CDS             complement(7237..7710)
                     /locus_tag="NOA01_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HCI0632754.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8111..8335
                     /gene="nrdH"
                     /locus_tag="NOA01_001559"
     CDS             8111..8335
                     /gene="nrdH"
                     /locus_tag="NOA01_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCI0632755.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8494..10653
                     /gene="nrdE"
                     /locus_tag="NOA01_001560"
     CDS             8494..10653
                     /gene="nrdE"
                     /locus_tag="NOA01_001560"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCI0632756.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10703..11668
                     /gene="nrdF"
                     /locus_tag="NOA01_001561"
     CDS             10703..11668
                     /gene="nrdF"
                     /locus_tag="NOA01_001561"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCI0632757.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11757..12896)
                     /locus_tag="NOA01_001562"
     CDS             complement(11757..12896)
                     /locus_tag="NOA01_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCI0632758.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13205..13918)
                     /locus_tag="NOA01_001563"
     CDS             complement(13205..13918)
                     /locus_tag="NOA01_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HCI0632759.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14172..14873)
                     /gene="nagB"
                     /locus_tag="NOA01_001564"
     CDS             complement(14172..14873)
                     /gene="nagB"
                     /locus_tag="NOA01_001564"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCI0632760.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15107..16639
                     /locus_tag="NOA01_001565"
     CDS             15107..16639
                     /locus_tag="NOA01_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCI0632761.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16830..>17007)
                     /locus_tag="NOA01_001566"
     CDS             complement(16830..>17007)
                     /locus_tag="NOA01_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCI0632762.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5278 a         3495 c         2910 g         5324 t
ORIGIN      
        1 tgaaaaaaca cattccacta aatttgatgc cggttttcct tgaggttttg ttccagcacc
       61 tcctttaatt tttttaagct cttgatctga acattgttta gcatttagtc tatttttttc
      121 ttccattacg attcccccta caaactataa atacttttat gtcactgatt caatatacct
      181 atttttctaa tcaaaaaatt ttgagacata tttaatatta taagtatcat taaaattgag
      241 atttatctcc ataatctgct cgatataaac catttattcc agcagttctt ccaatttcac
      301 tcaaatttct tattaattcc tcttgatatt cagtgaatct tattttatta gtataagcag
      361 taaaatatag ataattcacc aatctattta ttaaaggact gggatctaat tcatattttt
      421 gtttttctaa ttttaaatat accttttgta aaacctcaag aatatcagaa tatttaggat
      481 ctttacttat agatatatca ttatataatt catgaacaat ttgcttagca ttttttttca
      541 ttttagcact tccctggaat tgctccacct aaaaaaccac ctatagacat tcctgcaata
      601 caagtagttg ccttggccca atcgaccgta catttatttt tagtgcaata cactccattt
      661 ccataatatt ttccactatg agtggtaccc ccatagataa gttgtgtctc tttaatagac
      721 aaaattttta aatgtttcat aatctaatca cccctaaatt aatgttttta gactattatt
      781 ctaacattca ttaaatttca aaaaacaatt ccttcttgaa acgtagcaaa ataattcctg
      841 aataagaatt taatcacttt taagtatata aactatgtat attctttaat attttttgag
      901 aatgcacttc gtacataata aactttaatc acattagtct ttcataccta cgatcgatct
      961 ctttgactcg cacccataaa ttcataatag tttgtcggcg tctttgaacg tttcgaagat
     1021 gagctacgca ttctcttttt ttatagacaa aaaagataga gacgaacatg gagcttccaa
     1081 atttggatat ttgatccaat tttggaagct ccatttattt cgtgtctgtc tttttatttt
     1141 ttccagcttt cgactaattc ttggttctct ttgatccagt cttttgctgc ttgctgtgga
     1201 tcttttccat tgttgatttc caacataact ttttccatat ctttttctga ccaatggaaa
     1261 ttgtctaata ctttgtaagc ttctggctgg tcttctttca atccttttcg aaccatcgta
     1321 tggatctgct cttcgcttcc cattccgttt tcaggatctt ctaagtattt cagatcatat
     1381 ttggcaaaca tccaatgagg tgtccatcct gtaactacaa ttggttcgtg ttttttgatc
     1441 gcttgaccta gtgcaacggt cattgctcct gaagaagatg tctctacttt ccagtctttc
     1501 aagttgtcat atttttggat cgtgttttca gcagccgtaa ctacaccagc tcctggctcg
     1561 attcctatga ttttcttacc tgcctgatcg gttaactcat cgatcgaatt tacatccatg
     1621 taggctggca ctgctaaacc aacttttgct cctgtcaaat tggcacctag gtcttcgact
     1681 tgatctttat attgtgcata ttgtgaacca tgagttttag gtagccaagc tgaaaccatg
     1741 gcatctgact cgccatttga aacagatttc cacatgattg aattatctaa tggcgtcatc
     1801 gttacatcat agcccatttg tttcaatact tcaccgacta catttgttga agcaacttct
     1861 gtatcccatt caacataaga taagttgata cgttttgttt ctttggcaga tgaaaaactc
     1921 cctataccaa tcgcacctaa gatcactact actacagcac caatgatcag tccttgacgt
     1981 tttttacttt tctgtgttgt tgctcctgca gcttttttat tgtttggctg gttcaaatgc
     2041 tgtgtaaaac ggtcaatgat gattgctaag ataactaatg caaccccgtt gacaaatcca
     2101 ttcccgactt gtgctctttg caatgctgat aatacgccgc gtcctaagcc gggtgcaccg
     2161 atcatagagg caataacgac cattgaaagt gacagcattg ttgtttgatt gatccctgcc
     2221 atgatcgtac ttttagctaa aggcaattct agtttgaata gtttttgacg acttgtgctc
     2281 ccgaatgaat cagaagcttc tactaattct ttaggaactt gtcgaatccc taaattcgta
     2341 aaacgaacgg ttggtggcag agcaaagatg actgaggcaa atacccctgg aaccatcccg
     2401 ataccgaaaa atgcaactgc tggaatcaaa taaacgaatc ctggcattgt ctgcataaag
     2461 tcaaggatcg gtttgataat actttgggct ttactgcttt ttgccatcaa gatccctaat
     2521 ggtaccccaa tgatgatcga gatcacacta gataaaagaa caagtgtgac agtattcatc
     2581 aaatcattcc atagaccttg gttataaata aaccatagac cgattaatgt gaacaacgta
     2641 agtcctggac gtttcttaga aataaagaaa gctgctaccg tcaataacaa gataaacaaa
     2701 ggagctggaa tcactaacag taaatttgta atccccgaca tcactgcctg tccaatcgtt
     2761 tgtaaaaatg aaaataggcc agaaaaagtt gtcgtaaacc attctgtgat attttctacc
     2821 cagtttgcca ctggtaattg atagttcaat agattattca tgttcactca cctcgcttgt
     2881 ttctgctaat gcttcaatca cactacctcg gatgacgaca ccttttaatt ttccatcctc
     2941 tacaactgca agtggagtcg gtgagtcata aataatattg aaaatatcac ttaccaccat
     3001 gtctttccca atcaccgtca cattttcatc taggaagtcg atcaatggac gtttttgctt
     3061 cctagcttct aacgcttgat ctgcagtgat gatccctttc aggtgtctct ttctgtcaac
     3121 agccatcagc atgctgactt cttcattacg catccttgtc aatgcaacgt ttggtccatc
     3181 agattcgata ttcgtcgtca atgcaggtac catgatgttt tgagccgtaa gcactttgga
     3241 acgatcgact tcttccacaa attcacgtac ataatcattt gctggattgg tcaatatttc
     3301 ttcacctgtt ccgatctgca tgatttcgcc gtctttcatc aaggcgattc tgtccccaat
     3361 ccgtaaagct tcattcaaat catgggtaat gaaaataatc gttttttgaa cattggcttg
     3421 caggtccagt aattcgtctt gcatttctcg ccggatcagc ggatcaagag ccgagaacgc
     3481 ttcgtccatc agcaagatct caggatcatt tgctaaagct ctggctaggc caacccgttg
     3541 ttgcatccca ccagataact gactaggata ttggtcttta aatgaaagca aactagaatt
     3601 ttctaatgct ttttctgctt tttcttgtcg ttcttcttta ggtacaccac ggacttctaa
     3661 cccgtattct gtattttcca agatcgttcg atgtggaaac agaccgaagt tttggaaaac
     3721 catattgatt ttatgacgac ggacttctcg caattcttct ttgcttaact tcgatacatc
     3781 ttctccatca atatagatac tgcccgaagt tggctcaatc agacgattca atagacggat
     3841 caatgtggat ttcccactcc cggaaagccc catgatcacg aagatttctc cttcttttac
     3901 gtcaaaattc acgtcataca caccaaccgt tgcaccggtt ttctttaaaa tctccgtttt
     3961 cggctggtga tcattcatca tatctaaagc ggcttgtgtc tttttaccaa atatctttgt
     4021 taaatgtttt acttctactt ttaccaaaca aattctcctt ttttatgtaa attcattttt
     4081 ctttctgaaa aaagtgacca cactatgtta acatcatgta gtcactttgt caaaaatgaa
     4141 agaaatttgc ttgtccaaac tgaaaaaaac ttggctaaat ctttgtaaat aaaaggttta
     4201 gccaagttaa tattttttta atatttcaca caatcattct gcaagctgat gctccgggta
     4261 aaaaaagttt gtcatacgct gtagtgtata ttcatttcca ataaaatgaa cgatgtcccc
     4321 tgccgtaaat ttagcatagg ggccaggtga aacaagcagt tcttcattat gagaaattgc
     4381 gataactgtg gcgccagtcg cctgccagat attcaactcg ctaattgaac ggtccagatg
     4441 cattgctttt tctgataaag ataattcaaa cggaagtaaa ggattcatct tatgtattcg
     4501 cttcgtttga ttgaccagct gatccaataa ttgagaaaaa tgattcagct catcttgttg
     4561 cttctccaca cttgccatga tttctgcttt tgtatcttgg atggattgta catcttgata
     4621 ttgcaatagg aaatcttttg ctttttcctt cgaagcaaca aatgccccac ttccatgccg
     4681 tacttccatg attccaagat ctaccagcac attgatagcc ttgcgggtag tctcaggaga
     4741 agcattgtac gtactggcaa gtgttgagcg ggcatggatc ttctcaccca ctttatatcg
     4801 gttttcaacg atacgttcgg ctaaatcaac agcaatctgc tggtagcgcg ggcggacgat
     4861 ttttttcttt ccagacattt tctttcctct ttcttttttt actttccctc atcttactat
     4921 gctctagcaa aataagcaaa aaaggggaga atagcctctt gcaacgactg atcatcgctt
     4981 gatctgttcc ttttttactg ggcaagctgt ttgacagtca tcacaattct taccggattt
     5041 taaacgagta tagacaattg cgcctgcact tccaaagatt aacacactta ataagaatgt
     5101 cgccatccta ttcctctcct tctaatgaca atatagtata aaactggttc ttggattttg
     5161 gctgacggaa caaaacataa acgagtccta tcatcagtaa aatagcaaga tacgtcccta
     5221 acgctacctg tccaccctcc aataagtgtc ccaactgata aatcacaaaa ctaactaaat
     5281 aagctaaacc gcattgatag cctatggcga tccaagtcca tttcttggca ttcatttccc
     5341 gatggatagc cccaattgcc gcaaaacaag gcgcacaaag taaattgaag gctaacaatg
     5401 aataagctgc tgcgggagta tatgtgtgtt gtagcacttg ccagatttct ctgccatttt
     5461 cggatacttc cgcatgacca aacaaaatac caaatgtgcc aataatattt tctttagcga
     5521 ccaatcctgt aatcgctgcg actgttcctt gccagtttcc ccaaccaagc ggctgaaaaa
     5581 caactgccag gattcgcccg aaaaaggcta agatactttg gtcttcagaa acaggttgag
     5641 cataaaaatt ataagaagag ctgaaccaga tcacgatact agaaacaaag ataatcgttc
     5701 ctgctttttt aacaaaagac ttgctgcggt cataggtttg tcttaggacc gctgaccatt
     5761 gaggaaaatg atatgcgggc aattccatga taaaaggagc tgtatctcca gcaaacaaac
     5821 gtgttttttt caagctgatt ccagagaaaa taattgcagt cataccaaga aaataggcag
     5881 aaggagctac ccaagattga tgaggaaaaa aggcccctgc tactaaggca ataatcggca
     5941 actttgcaga acaaggcatg aaggttgtga ccatgatcgt caaacgacga tcttgctcgt
     6001 tttcaatcgt gcgactggcc atgacgcctg gcactccaca tcctgtagca atcagcatag
     6061 gaatgaagga ttttcctgaa agaccgaatt tcctgaaaag tcgatccatg acaaaagcga
     6121 tcctcgccat atatccgcag tcttccagaa agctcaagca taaaaacaat ataatcaatt
     6181 gcggtagaaa tcctaacact gctcccacgc ctgcgatgat gccgttcaag atcagctcct
     6241 gcatccatcc cgctacttgc cattcagcta acaatctgcc aacatgttct ggtaaccatg
     6301 taccaaacaa tgtgtcattg atccaatctg tccccattgt tcctactgtt tgaatagcca
     6361 gataatagat cagccacatc acaaatgcga agatcggcaa ggctaaccaa cgattcgttg
     6421 caatctgatc gattttgtca ctcatcgata attggaaaga atcatttgac acaacacaga
     6481 gtgtacatag ttgggtaata aattcataac gttcattgac cagaatcgtc tcactatcgt
     6541 cgtccaatag tttctctgtt aactggatca gttgctcgat ctctttttgc tgagaaactg
     6601 ctatctcgta atcctctctt accttctgat ctcgttcaaa atatttcatt gcataccagc
     6661 gtttcttgcc gctttgttct cctagtacat cttcaatttc atgtaaagct gcttctaaac
     6721 gtggatcata tgtacaggga acaggaatag aaggccgctt ttttgcagac tcctgaatct
     6781 tttcgattag tttttcgatt ccttgctttt tcaatacgct gattggtaaa actggtacac
     6841 caagaccata ggaaagcttt tctagattga ttttttttcc ttcttttttg atgacatcca
     6901 tcatattcag accaatgatc ataggaatgt ctaaatccaa taactgcgta gttaaataaa
     6961 gatttcgttc taaattggta gcatcaataa tgttgatgat cagttcaggc tgttcctgaa
     7021 ccaaataatc tcttgcaatc acttcttctg gtgtataagg agacaaagaa tagatgccgg
     7081 gtaggtcctg aatatgaatg ttcggatttt ttttcaacga tccttctttt ttttctactg
     7141 ttacgcccgg ccaattccct acatattgat tcgaaccagt caagagattg aacaagctag
     7201 ttttcccgct gtttggattt ccgactaacg cgatattcat tctttttcct ccctttgtat
     7261 caagattagt tcggcctctg actttcgtaa cgtcagttca taccctcgca ctgtcagttc
     7321 gattggatct cccagagggg ctagttttcg gataactatt tttacatttc gcgtcaatcc
     7381 catatccatt aaccgtcgct tcactgcgcc ttgtccgtgg attgctacga cgatcccagt
     7441 ctctcctgct tttaattgat caagagaagt ccagctttca gaggaagcag tctttttatt
     7501 gacaaagatt tgacctagta tttcccgact tactgccact cgatgacttt ttaccagtaa
     7561 aatggcattg ttgcctgata aattgacgac gacgatttta cttccggcaa cgatcccgat
     7621 attttgcaaa tggcgttttg ttttttcttc tgtttcgatt cgttcgactt gataaacgaa
     7681 attctgcttt gcctgcgcta aggtctgcat attgattcct ctcttgtgaa ataaatcatg
     7741 tattaaaatc attcattata tgttctaatt ttcttacgtg ttacgcaaat aaatgagaat
     7801 cattctcaca tagaatactc tcggtaattg agaatgtcaa ttatacttgt gatattatga
     7861 gcgaatcttt agtattgata gataaaaatg agaaaaaaat atttcttcat agttttccac
     7921 aattttttct ctattaaatt tgctattttc ccacacgata cttgttttca gctctttttt
     7981 caaaaacaca aaacttagta ttccacagtt tccacaacac aatatgtagt gtttgttgtt
     8041 tgactttttt actaagagtt ttatactatc tattatagat aataagaatg tgttagagga
     8101 ggcactcacc atgaacgtaa aaatcttttc taaaaataat tgtatgcaat gtaagatggt
     8161 caaacggttt ttaacagaaa atcaaattgc ttttgaagaa gtcaatatcg atgaacagcc
     8221 tgaagcagta gactggttga aagcacaagg attccaaagt gttcctgtca ttacttctga
     8281 tgcagaaaca gttatcggat tccgtcctga tcagttgcgt aaacttgcca gctaacatga
     8341 ctaaaagaat caaaacgtga tacatggatt tgaagggctt gagacagcac cagtttttgt
     8401 cgcaaacttc caaatccttt tttaaagctt aaaaacaatg caatgatttg caatcaagca
     8461 tcatcaaata cgaaatgaag gttgggcgta cgtatgagtc ttaaaaattt acaagatgtc
     8521 agttatttca agctgaataa tgaaatcaat cggccagtca atggtcaaat ccctttgaat
     8581 aaggataaag aagctttagt tgcctttttt gaggaaaatg tcaaaccaaa tacgatgact
     8641 tttccatcta ttatggaaaa aatcaattac ttgatcgatc aagaatatat agaagcagaa
     8701 ttcattcaat tgtattcacc atcatttatt gaaaagctat atcaattttt ggacgagcaa
     8761 catttttctt tcaaatcttt tatggctgct tacaaatttt attctcaata tgcgttgaaa
     8821 accaacgacg gttccgctta tctggaaact tatgaagatc gagtagcctt caatgcgctt
     8881 tactttgcga acggggatga agaattagca cttgctcttg cagacgaaat gatccatcaa
     8941 cgatatcagc cagctacacc ttctttcttg aatgctggac gtaaacgtcg gggcgaattg
     9001 gtgtcttgtt tcttgactca agtcactgac gatatgaact ctatcgggcg ctcaatcaac
     9061 tctgctttgc aattgtcccg tattggtggc ggcgtaggga ttactttatc aaatcttcgt
     9121 gaagccggcg ctcctatcaa aggatatgag ggtgctgcaa gtggtgtcgt tccagtcatg
     9181 aaattattcg aagatagttt cagctattct aatcaattag gtcagcgtca aggagctggc
     9241 gtggtttatt tgaacgtttt ccatccagac attattgctt tcctttcagc aaaaaaagaa
     9301 aatgcagatg aaaagattcg cgtaaaaact ctttctcttg gcgtgatcgt acctgataaa
     9361 ttttatgaat tgacacgtaa aaacgaagat atgtatttat ttagtccata tagtgtcgaa
     9421 aaagaatatg gtgtcccatt ctcttatgta aatattacag ctgagtatga tcatctagtt
     9481 gcaaatccaa acattcgcaa acaaaaaatc aaagcgcgtg atcttgaaaa tgaaatctca
     9541 aaattacaac aagaatctgg ctatccttac gtaatcaata ttgatacagc gaaccgcgag
     9601 aatccaatcg atggcaagat catcatgagt aatttatgtt cagagatttt acaagttcaa
     9661 acgccttctg tgattaacgg caaacaggaa tatgaagtat taggaacaga tatctcatgt
     9721 aacttaggct cgacaaatat cgtgaatttg atgcaaagcc ctaactttgg tcaatctgtg
     9781 cgtacgatga ctcgtgcatt gacatttatt acagatgcat ctgatatcga tgtcgtacca
     9841 ccgatccaaa acggaaataa gctgaatcat acgattggat taggtgccat gggcttacac
     9901 acttatcttg caaaagaaca aatggaatat ggctcagagg attcattaga ctttacaaat
     9961 atttacttca tgctgttgaa ttattggaca cttgttgaaa gcaacaatat cgctcgtgaa
    10021 agaaaacaag ttttccataa ttttgaaaaa tcagcttatg ctgacggcac ttatttcgaa
    10081 aaatacgtaa ctggagaatt ccagccaaaa tcagaaaaag tcaaagcatt gtttgaaggc
    10141 atttttatcc caactgctga agattggaat gctttgaaac aagcagtaat gaaagatgga
    10201 ctgtatcatc aaaatcgctt ggcagtagca ccaaatggct caatttcata tatcaacgat
    10261 acgagtgcaa gtattcatcc tatcactcgc atgatcgaag aacgccaaga gaagaaaatc
    10321 ggtaaaattt attacccagc gccttatcta tccaatgaga cgattccgta ttacacttct
    10381 gcttatgata tggacatgcg taaagtaatc gatgtttatg caacggcaca acaacacgtc
    10441 gatcaaggca tgagtttgac tttattcatg cgttctgaaa tcccagaagg catttatgaa
    10501 tggaagacaa caacgaagca aacgacacgt gatttgaata ttttaagaca ttacgctttc
    10561 cacaaaggaa tcaaatccat ttattacgta cgtaccttta ctgatgatgc agaagaaatc
    10621 ggaagcaatc aatgtgaaag ctgcgtgatc taatatcata gatagattcg cttattcata
    10681 ttttaattaa aggagcattc tcatgtcaga aacatattat gaagcaataa actggaatga
    10741 aattgaagat atcattgata aatccacttg ggagaagctg acagagcaat tttggctaga
    10801 tacacgtatc ccactttcaa atgacctaga tgattggcgt acattgtctc aattggaaaa
    10861 agatacagta ggacatgtat ttggcggttt gacactgcta gatactgtcc aatctgaaag
    10921 tggcatggat cagttgcgta aagatgtccg cactccccat gaagaagccg ttttgaacaa
    10981 tattcaattc atggaatccg ttcatgccaa aagttattct tctattttta gtacactgaa
    11041 tacgaaaaaa gaaatcgaag aaatttttga ctggacaaat acgaatcctt atctgcaaaa
    11101 aaaagctgag cgtatcaatg aaatctacaa acacggtaca ccgctagaaa agaaaattgc
    11161 cagcgtcttt ttagaaacat tcttgttcta ttcaggtttt tatactcctc tttattattt
    11221 aggaaacaac aaattggcta acgtagcaga gatcatcaaa ctaatcatcc gagatgaatc
    11281 tgtacatgga acctacatcg gttacaaatt ccaattagga ttcaacgagt tacctgaagc
    11341 agaacaagaa acgttaaaag actggatgta caatcttctt tatgagcttt acgaaaacga
    11401 agaacgttat acagaagaat tatacgatcc aatcggctgg acagaagaag tcaagacatt
    11461 cctacgctat aatgccaaca aagcattgat gaacctaggg atggatccac tctttccaga
    11521 tactgccaat gatgtcaacc caatcgtaat gaacgggatt tcaacaggca caagcaacca
    11581 tgacttcttc tcacaagtcg gaaacggcta tttgttaggt catgtagaag cgatgaaaga
    11641 cgacgattat ttgattggat tggaataagg atttatttta attcaaaaat ttttcgaaaa
    11701 ataaaaagag tttagcgtac agatatttta aatgtacact gaactctttt ttatgcttat
    11761 tcacttgttt cttccagcac ttttggactg tctaactttt ccgaaacatg ttccattctt
    11821 ctcaataaat agattttccg gatatttaat attatcgtct taatcactgc atagacaggt
    11881 atgcaaatca gcatgcctaa tattcctgcg acacttcctg ctccaatcaa taccaagatg
    11941 atcgtcaaag gatggatatt cattgatttc ccaaataaca acggcttgat caaatttcca
    12001 tctaattgtt ggatcactaa aatcgacaac gccatataca aagcttgaaa tggagagata
    12061 aacaaaccga caatgattgc tggtaccgct ccaataaaag ggccgacata cgggataata
    12121 ttcgtgattc cgcaaatgac cgcaagcaac aagctataag gctgatcgaa tattctcatt
    12181 gccaaaaagc tcattacacc aataatcaaa gcatctaaga tcgtactgct gatataggca
    12241 gataatgttt cgtttaattc ttttaccgtt tggcgtaact ctttacgaat cgctttcgga
    12301 aagaagtgag acaacgcatc aataaatttg tgcccatcct tgaacataaa tagcaaaata
    12361 aatggaacgg taaataacaa aataaaaaat tgcatcataa atgaaatgat ttttgagaca
    12421 cttgcagata aactgactaa tatgatattg aacaaattac taagagataa atttgctgat
    12481 gctaattgtt cttccaaatt gaaattttta aactcttccc gctgcgccaa ttcatttaac
    12541 catttgcttg tttcatctgc atacattggc aagctctggg tcaattgaat tgtctgttcc
    12601 attaactgag gcactacatt catgacagtt aaaacaacta aaatgattac gaccaaaaac
    12661 gtcaataaaa aaccgaacac tctcgggatc cgatgctttt cgagaaaaac aacgattgga
    12721 tcgaacatat aatagataaa accagcaaca agcaatggta aaaaaattgc cgaaagcatt
    12781 ttcaatattg gactaaaaat attcggcatt tgtaaaataa agtacacgcc gatgatcgtg
    12841 agtactagct ccactgtcca gaaaaataat tttgattgtt taaaacgtga ttgcatacat
    12901 ctcctccaag gtcctttgat acttctattc tatcatttaa caaagagtta cgcgctgaga
    12961 ttttcatttg cttaaaaaaa acaaaaaaaa taaacatatt ttaatgagta gacgagtact
    13021 taaacaaaaa cacggtttgg agtagtgaca aaaattatac tgcagtccct ttttccaaat
    13081 aaacggtgtt caaaagcttg catcttcgta ataagcccag ccaaacgaaa aatataagga
    13141 actattttta tttggctggg cttattactt gatgtaggac atctatttta caacctcaga
    13201 tcatttattg cgcaatactg actaaatgca actttgtgat atttttgtgt acggacaact
    13261 cctcgatcac atcggcataa gtgagccctt ttggaagatc aatcgtatag atatttttgt
    13321 acactcggta gtcatcaacg aattttacgt caaaattcac atcttcgatt acgatatttt
    13381 tctcttcaaa ataatcggta agaaaatctt tggtttcttt tctatggata aattggattt
    13441 ctaacttttt agtcgtaggt acatggatca cgcgtttcac tactgtgagt gcaatagcaa
    13501 tcccgataaa actgatgatc gcaatggaat agtaacccat gccgatggcg atccctaatc
    13561 cagcaattgc ccataaagaa gcggcagtcg taagtcccgt caccgattgt ttcgtgacga
    13621 tgatcgttcc cgcgcctaag aaaccgacac cactcaccac ttgagcaatc agccttgctt
    13681 catctgaacg aatcgttcca gcaagctgtg gatactctaa agcatctctt aatgcaccac
    13741 tagcgatctc aacttggatc aatgcaatga tacatgctcc catacaaact aaaatatgcg
    13801 tacgcatacc tgctggacga tttttatatt gtcgttcaaa accgattgct ccgccaaata
    13861 gcatagcgat ccctaaacgt aaaataatct ctggaattgt caataccata tcattcatct
    13921 aaacaccctc cagtccaata tatcatgtct gaaaggagca gacaaaaaaa gtgcaatttt
    13981 tacctcttac gaaaaaagga ctgtgacaaa atttttgtca tagtcctttt tatgaataaa
    14041 cggtgttcaa tcagctgacc ctcggtatta ctcaacaaac ggcaaaatat ggggagctat
    14101 ttttcctttg tcttctctaa tactcaggtc aaaacgctga tttcacaacc tctttagcct
    14161 actattgaaa attacaactt acttgctgct tcttcatcga tgatcacaac aacattgtcg
    14221 tgattttgca atacacttgc tggcaaatga ttcgttacag gtccttcaat catccctttg
    14281 attgcatcag ctttgctttc gccaaatgcc atcaagacga tttctttact tttcataatt
    14341 gaaccgattc ccattgaaac agcttttgtt ggtacatctt ctgctttatc gaagaatcgt
    14401 ttgtttgcat tgattgttga ttctgttaag tcaactactg aagtcgttcc gtcaaatgaa
    14461 gcaccaggtt cattgaaacc gatatgtcca ttttgcccga ttcctaagat ttggatatcg
    14521 attggatgtg catcgatgat ttgatcatac tttttgcatg ctgcttccag atcttctgct
    14581 tttccatctg gtacaaacgt ttctttgaat ggttttttat caaataattg gacatccatg
    14641 aaatgacggt agctttgttc atctgaaccg cctagtccaa cgtactcatc taagtttaca
    14701 gaagtcattt cactgaaatc caaatcgctt gaagtcattt ctttatataa agtaactggt
    14761 gtgcttcctg ttgccaatcc taatactttt gcacctttgt tcataccttc tttgatgatt
    14821 tcaaatgctt tttttccgcc ttcttcagcg ttttttacac gaatgatttc catagtgatt
    14881 gctcctttat attttggtat agcccaatta tagcattctt tttttctttt tacaataatt
    14941 agtatagacc aaattgcatt tttttcattt tcttgacaat aaataggaat ccgtgttagg
    15001 tttcataaga tggttcaatc aaacaatcca tgttgcatga aaaccaagca agtgattcac
    15061 tgtggacgct ttatgtctgc agttttttta tgaaaaagga gttattatga gtatactgac
    15121 attaaacaac attacccagc aatttggcga caaaatcctc tatgaaggtg tcgatctgca
    15181 attaaatgct ggcgaacatc ttgggctgat tggacaaaac ggagccggta aaagtacatt
    15241 gattaaaatc atcactggtg agatcttacc agatgacgga cagatccatt ggcaaaaaaa
    15301 tattcacaaa ggttacctcg atcaatatgt ggaagtcgat gaaacattaa caatcgaaga
    15361 atttctaaaa actgcgtacg caaaagaata cgaaaaggaa gaaaaaattg cttttcttta
    15421 ccaaacatat agcgagacct tcgatgattc cttgctggaa aaagctggga aactccaaac
    15481 ggaattagac caaggagtgt tttatcaaat cgacacctta gttgctgaga tgagcagcgg
    15541 gttggggatc gatgtcttag gtttagattc actgttgaaa aatctaagtg gtggacaacg
    15601 ttcaaaggtg attttagcaa aactactttt agaaaaccca gatgttctga tcttagatga
    15661 accaaccaat cacttagatg atcagcatat ccaatggctg acgcagtttc ttcaggattt
    15721 tgacggtgcg tatatcgtga tttcccatga ccaagaattt cttgatcaga tcaccaccca
    15781 tatcgcagac atcgagtttg gaaagattac taagtatact ggccatctaa aacaagcatt
    15841 gaaacaaaag gaacaaaatc gcgaaagcta tttgcgccaa tatcatgctc aacaaaaaca
    15901 aatcgaaaag accgaagctt atattcgaaa atacaaagcc ggttcccgat caacgatggc
    15961 aaaaagccga caaaaacaat tagataaaat cgaacggctg acgcctccag cttcagcaag
    16021 taaagctgtc tttgattttc cattctcgcc tattgtcacg acattagcag tggaaacaac
    16081 tgatttagtc atcggatatg atcgaccgct acttgcaccc atcaatttga ccatccgctt
    16141 tggtgaaaat attgccattc gaggcttcaa cgggattgga aaatctactt tattaaaaac
    16201 actgatcggt gagattgaaa aaataagtgg cgatttccac ttcccagata acacaaaaat
    16261 caattacttt tcacaagatc tgtactggga aaatccatta gaaacaccac tacaatattt
    16321 aagcagtcaa tttccaaaag cgactatcaa agaattacgt cgccaactag ctaaagcagg
    16381 gttagttaat caattagcaa gtgaacctct cactacatta agtggtgggg aacaaactaa
    16441 ggtgaaactt gcacagttga caatgaacca aggtaattta ctgattctcg atgaaccgac
    16501 gaaccatatc gatcaagaaa cgaaagaaag cttacaagac gggatacaga aatttccagg
    16561 aacagtaatc atcgtctctc atgaacaaga attttatcaa gatcttgtgc aacgagtcat
    16621 tgagattgaa ccaaaataaa cgtttgattg aatagttgaa aaaaaggagc tgtgacaaaa
    16681 gttttgtcac aactccttct tctgaataaa tggtggccaa ttagctgacc ttcggtatta
    16741 gctcaaaaaa cggcaaaata tgaggagcta tttttatttg gctattctta ttacttgatg
    16801 cagaacagct tttcacaacc tcttttcgtc tattttttat tttcgagatt tctctttcct
    16861 ttaaaaacaa ctgtttgaaa gaagaacgca catccttgaa aaacaagccc gctaaccgtt
    16921 acacctaacc agccatactg ctgccacatt gtcgtcccta gcaatgaacc agccgcacct
    16981 ccaataaagt acataaacat aaataca
//
LOCUS       DAIGOK010000061        16265 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.061, whole genome shotgun sequence.
ACCESSION   DAIGOK010000061 DAIGOK010000000
VERSION     DAIGOK010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.061"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(95..1030)
                     /gene="murB"
                     /locus_tag="NOA01_001567"
     CDS             complement(95..1030)
                     /gene="murB"
                     /locus_tag="NOA01_001567"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCI0632763.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(1103..2023)
                     /locus_tag="NOA01_001568"
     CDS             complement(1103..2023)
                     /locus_tag="NOA01_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCI0632764.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2136..2888
                     /gene="xth"
                     /locus_tag="NOA01_001569"
     CDS             2136..2888
                     /gene="xth"
                     /locus_tag="NOA01_001569"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCI0632765.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            3752..4285
                     /locus_tag="NOA01_001570"
     CDS             3752..4285
                     /locus_tag="NOA01_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HCI0632766.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(4775..5299)
                     /locus_tag="NOA01_001571"
     CDS             complement(4775..5299)
                     /locus_tag="NOA01_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCI0632767.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(5292..5765)
                     /gene="tsaE"
                     /locus_tag="NOA01_001572"
     CDS             complement(5292..5765)
                     /gene="tsaE"
                     /locus_tag="NOA01_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCI0632768.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(5888..6868)
                     /gene="pta"
                     /locus_tag="NOA01_001573"
     CDS             complement(5888..6868)
                     /gene="pta"
                     /locus_tag="NOA01_001573"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCI0632769.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(7021..7701)
                     /locus_tag="NOA01_001574"
     CDS             complement(7021..7701)
                     /locus_tag="NOA01_001574"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCI0632770.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            7827..8696
                     /locus_tag="NOA01_001575"
     CDS             7827..8696
                     /locus_tag="NOA01_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCI0632771.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            8722..9237
                     /locus_tag="NOA01_001576"
     CDS             8722..9237
                     /locus_tag="NOA01_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632772.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(9284..9877)
                     /locus_tag="NOA01_001577"
     CDS             complement(9284..9877)
                     /locus_tag="NOA01_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCI0632773.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(9902..10081)
                     /locus_tag="NOA01_001578"
     CDS             complement(9902..10081)
                     /locus_tag="NOA01_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632774.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(10158..10667)
                     /locus_tag="NOA01_001579"
     CDS             complement(10158..10667)
                     /locus_tag="NOA01_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632775.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(10763..11143)
                     /locus_tag="NOA01_001580"
     CDS             complement(10763..11143)
                     /locus_tag="NOA01_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCI0632776.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            11473..12222
                     /locus_tag="NOA01_001581"
     CDS             11473..12222
                     /locus_tag="NOA01_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HCI0632777.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(12406..13683)
                     /locus_tag="NOA01_001582"
     CDS             complement(12406..13683)
                     /locus_tag="NOA01_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632778.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(13762..14406)
                     /gene="pcp"
                     /locus_tag="NOA01_001583"
     CDS             complement(13762..14406)
                     /gene="pcp"
                     /locus_tag="NOA01_001583"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HCI0632779.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(14605..15549)
                     /locus_tag="NOA01_001584"
     CDS             complement(14605..15549)
                     /locus_tag="NOA01_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HCI0632780.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(15551..16237)
                     /locus_tag="NOA01_001585"
     CDS             complement(15551..16237)
                     /locus_tag="NOA01_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HCI0632781.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         4681 a         3378 c         2879 g         5327 t
ORIGIN      
        1 tgtacaagat ttgcgccatt tgcgtaaaca accacaaaaa agagcaggac ggccaccgtc
       61 ctgctctttt acttatttta agttccatta cttattaatt ggctaaactt ctgcttgttt
      121 ctgatattgc ttgtttttct ccaatgatcc gcacttctgt ttctaaagac acatcaaatt
      181 tttctttgat tacttcttgg atgtgcgcaa tcaattcaat ataatctgtc gctgtcgcat
      241 gatcgatatt gacgatgaat ccagcatgtt tttcagagac ttgtgcaccg ccccatttca
      301 ggccttgcaa tcctgcttgt tggatcagct gacctgtata gtgtcctact gggcgcttaa
      361 agacacttcc gcatgaaggg tattctaatg gctgttttga ttgacgcaat tcggtcaact
      421 catccattct tgctttgatt gcttcataat cccctgtttg caaactgaat cgagcttcca
      481 atacgatagc acgtaattct tggactttac tgtgacgata agaaaatacc atttcgtctt
      541 ttgtcaaagt tttcaatgtt ccatcttcaa gcaaaagatc cacttcagca aaaacatctt
      601 tgatctctcc gccatacgca cctgcattca tataaaccgc tccaccgaca cttcccggga
      661 tgccgcaagc aaattcaaat ccagtcaatg attctgccaa agctgcataa gtcgtatcaa
      721 tcagtgaggc acctgcttcc gcaatgacca tcgttccttt gacatgaatc tgtttcattt
      781 cagtcagcat gatgactaca ccgcgaatcc cgccatcttg aacgatcaga ttgcttgcat
      841 ttcctaatac catccaaggt atatcatgga ttcggcaata atcgatgatt tgctttactt
      901 cttcttgttt tttcgggaat gcaaggacat cagctggtcc acctgttttc gtaaacgtat
      961 agttcatcaa tggttcatca aacaatatag taatcgtggc taagctttca actatttctt
     1021 ctttattcac tgaggatttt cctttctttt ctcaaacgtc ctcattattc tatcacgctt
     1081 aatacaatct tgccagtctt gttcaaagtt gatgacggta gtgatcttta agcacttcct
     1141 cacataattc atgacttctt acaacttctt tttgtttaag caattctgtt tccgatttac
     1201 ctaaacggat ctgagaaaag aaggcctcta ccattggctc aaacccgcgt ttgactagcg
     1261 tgtttgtcca gtcgccaaat tctttcactt tttttccatc agcatcttga atgacaagct
     1321 gggtcagatc gtttacttca taagtaccat ttttacttgt tacttgatat ttttctatat
     1381 tagcgccact tgctaaatcc atcgttaaaa tagccgtttg attttgtgtt tctaattgta
     1441 aaatggcata ttccaaaaat tcctttgttt ctcttatctg gtattttgtt ctcaaaatgg
     1501 gttcatctaa taaatacaca gctgtatcaa taagatgtaa aaacaaatca taaaggacga
     1561 actctgtcgt ttcttttgcc gctattcgat ttttttgcag gtggatcaac cgcttatctg
     1621 ggatacgttt caattcttct accataggag caaagcgccg gttgaaaccg atcatcaaaa
     1681 ttatttggtt cttatcagct aattcttgca attcacgaac ttcatttaga tggacactca
     1741 gtggtttgtc aatataaaca tggataccat tttctagaca ttttttggct aattcataat
     1801 gtacttttgt tgctgcgtgg atcatacatg cttcgatttt ttcttctaaa agctcatcga
     1861 tcgtttcata tagatgttgg aacccgtact ctttttgaat tctttctttt gtttctttat
     1921 ttcttgttgc aaaataaaaa tcaagtgttc cttgtttttt cgcataagtt ggtaaatatg
     1981 ctttttctgc gatattccct acaccgatta caccaatctt catctacttt cctcctggat
     2041 catttagtta ctttcatctt acaccatttt ttactaaaat gaggtacaat agtatcgcaa
     2101 aggtaaggaa attaattcga aggagtgtgc ttttattgaa atttatctct tggaacgtaa
     2161 acgggcttcg tgccatcgta aataaaaatt ttttagaagt ttttcatgag ttagacgccg
     2221 attttttctg tctgcaggaa acgaaactcc aagcaggaca aattgattta gatcttccag
     2281 gttattatca atattggaat tacgcggaac gaaaaggcta ttccggcaca gcgatttttg
     2341 ctaaaaaacc agcattgaat gccacttacg gaatgggtat cgatatccat gatacagaag
     2401 gccgtctgat tactttggaa tattccgatt tctttcttgt aacctgttac acgccaaatt
     2461 ctcaaagcga attaaaacgt ttagattacc gattagaatg ggaagaagcc ttttataatt
     2521 atttggaaaa tctaaaaaaa caaaaaccag tcatcgtgtg tggggatcta aatgtcgctc
     2581 atcaaaagat tgatttgaaa aactggaaaa cgaatcaaaa aaatgctgga ttcactccag
     2641 aagaaagagc ggctctttct cgactgttgg ataacgggtt tatcgataca ttccgttatt
     2701 tttatccaac ccaggaaggt gtctactctt ggtggaacta ccgattcaat tcaaggaaaa
     2761 acaatgctgg ttggcggatc gattactttt tgacgagcaa agatttagag ccaggactag
     2821 ctgatgcaaa gatccatacg gacattatgg gcagtgatca ttgtccagta gaattagatt
     2881 tgaaataaag aggttgtgaa aaagtcgttt agtttcgagt attaagaaaa attttgtgaa
     2941 aatggctttc cacattttgc ataaatttgg cataataccg agaaactggc ttttgaacac
     3001 tgtttatcga gagattgtga aaaagctgtc ctgcatcaag taataagaac agccaaacaa
     3061 aaatagttcc tcatattttt cgtttggttg ggcttattac cgcagataca agcttttgaa
     3121 caccgtttat taagaggttg tgaaaaagct gcactgcatc aagtaataag aacagccaaa
     3181 caaaaatagt tcctcatatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt
     3241 gaacaccgtt tattaagagg ttgtgaaaaa gctgcactgc atcaagtaat aagaacagcc
     3301 aaacaaaaat agttcctcat atttttcgtt tggttggact tattaccgca gatgcaagct
     3361 tttgaacacc gtttattgag aagttgtgaa atcagcgtcc tgacctgagt attagagaaa
     3421 caaatggcaa aatagttcct catattttgc tattttttga gctaataccg agggtcagct
     3481 gattgaacac cgtttagtgg agaagttgtg aaaaagtcgc ctactttcga gtcgtaagaa
     3541 atttttttga aaatggttcc tcacaacttt gcataaattt gacttaatac cgagaagtca
     3601 tgatccccat ctaactccaa atcacaagga atgagttcaa aaactagctt ttctcaacaa
     3661 atattacata aaaaattcta tgttttttgt taaatttatt tgcttttttc aaaagtatgt
     3721 tacagtaatt tcccaaggag gttttacaga aatgaatttt gttgccatgg attttgaaac
     3781 tgccaatcat cagccttata gtgcttgttc tcttgcgcta gtcatggtaa aaaatagcca
     3841 gatcgttgat gaattctata cattgataca gcctgagact ccttttttct ggcgaaatgt
     3901 ccaaattcac ggaatccatc aagaagacgt acgcaatgcg ccgaaattcc cagaagtctg
     3961 gcaaactatc caaccatatt ttcaaaaaaa tcgtttagtc gttgcacata atgctgcttt
     4021 tgatacaaag gtcttagcgg gatgcttaga ttattatcat cttgaacagc ctaattatct
     4081 ttctttatgt acagtgaaaa gtagccgtcg cctgtttcct gaatttccaa atcatcgatt
     4141 gaacactgtg tgcgaaaatt tgaacatcca attagatcat caccatgatg cattggaaga
     4201 tagtcgtgca tgtgcggaaa tcctgcttta tcaagaaaaa aaattcggga cagatccatt
     4261 gaaaaaactt gtgactataa agtaaaagaa tacttatgaa acacacaaag aggtcgtgag
     4321 cctgccgttt cactccgagt cacaaggagc aatttcaaaa aatagcttct catgtttatt
     4381 aaatattgtg actggtgacc gaggagctgg caggagaaca ccgtttattg gagaggttgt
     4441 gaaaaagctg ttctgcatca agtaataaga acagccaaac aaaaatagct tctcatattt
     4501 ttcatttggt tggacttatt accgcagatg caagcttttg gacaccgttt accaagaggt
     4561 tgtgaaatca gcgttttgac ctgagtatta gagaaacaaa cggcaaaata gttcctcata
     4621 ttttgccgtt ttttgagcta ataccgaagg tcagctgatt gaacaccgtt tattcaaaaa
     4681 aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg ccttctcaaa
     4741 aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa ctcattttat
     4801 atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc gcttcgattt
     4861 ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga acgtcaagct
     4921 ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct agcataagag
     4981 tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct cccacgtgag
     5041 acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc ccttcataga
     5101 ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct tcttctaagg
     5161 tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc tccttattaa
     5221 cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc cgcaagatga
     5281 tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg tttgaaggtt
     5341 tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc tgtatcactt
     5401 ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag atttccccat
     5461 tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc tgctccgcta
     5521 gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact atattctcta
     5581 ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg ggcaattcct
     5641 ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt gtcacctggc
     5701 attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc tgatagttcg
     5761 atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg taatgaagtt
     5821 tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc tagttcgcgt
     5881 gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta tatacatctt
     5941 cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt agaattggtc
     6001 cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt cctgactgca
     6061 attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc gctttcagtt
     6121 gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc atgtattgtg
     6181 gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct ggcgatttcg
     6241 ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg aacaattctg
     6301 ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga ttcacgttga
     6361 tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg atcatcgctc
     6421 cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg cgaacagtgt
     6481 cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata tacgtaagca
     6541 tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg gatgcctttc
     6601 catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa ttatcagggc
     6661 tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt cttatttctt
     6721 cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg gcagctgcgc
     6781 ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg cgaacaattt
     6841 taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt atatgtatta
     6901 tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc aaaaatgctg
     6961 gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag cgctgtttta
     7021 ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa ggagcgcatc
     7081 attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata acggactggg
     7141 atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt tttcttgtgc
     7201 cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt tttcaatgat
     7261 cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc cttcccgaac
     7321 ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta gatttccatg
     7381 ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc ttaacgatgg
     7441 cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat tttctccatg
     7501 ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca attccaatgc
     7561 ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt tttttaaaaa
     7621 ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt cttgccaact
     7681 gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac tcacattttc
     7741 ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa actaggaaca
     7801 agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat atggacggca
     7861 cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata cgttttgcaa
     7921 atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa gctcgcgcag
     7981 ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca caggtatttg
     8041 ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca atctctgttt
     8101 tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat ggtctttatt
     8161 ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc catttacctc
     8221 accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta ttgcacatca
     8281 catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt ttaaaaatta
     8341 tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag atttctcttt
     8401 taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac cataaaaacg
     8461 ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct ttagaagaag
     8521 tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca ggagtcagtt
     8581 tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca acggacacaa
     8641 accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac ttgtagtttt
     8701 atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca gcttggacgt
     8761 gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat ggttggtcgc
     8821 tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct cgttgctcac
     8881 atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga gttcgaaaag
     8941 gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat tcgtagactt
     9001 cactggacag tccaaggtga tatctataat catcaatatc aaatccatca tttcaatcag
     9061 caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt attggatgtc
     9121 tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga ttactggctc
     9181 taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt aagttaaaaa
     9241 gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata ttcttttgca
     9301 tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag tcatggcgtt
     9361 cgtataattt tttagccttt ggattttttt catccacact tagaccgatg acggagcgat
     9421 tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg ccttgtcctc
     9481 taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac gtttctttat
     9541 caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt accagtggct
     9601 gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca actttaccgt
     9661 ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca gtgattgctt
     9721 cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc agttccatat
     9781 cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt ctcgcaaaac
     9841 gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa tcgcaaaatc
     9901 gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta acgattcttg
     9961 ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta tgttcgacta
    10021 cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc attttttcca
    10081 tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt gtaaaaaaga
    10141 gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc tccatctatt
    10201 aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc tttcactttt
    10261 gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg tttgaactct
    10321 tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt tcgtacggtt
    10381 gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa ctggtattca
    10441 tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat agcagatatg
    10501 ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga gatccgatat
    10561 tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt cataccggta
    10621 atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct tcattctcct
    10681 gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat gaaacgaaaa
    10741 aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg tctggatcaa
    10801 agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct gattcgatcc
    10861 ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa gcaagatgac
    10921 gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt ccaaaaatct
    10981 ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct ttgcggtaat
    11041 tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat ttctgataat
    11101 ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca ctcctattct
    11161 tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc agattgaaaa
    11221 aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag taggtcctcc
    11281 aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat tttcaagaaa
    11341 acaaacagaa aattatcata acacaaatta tttataattg aaagactagt tttttttcaa
    11401 gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta gtgtcttaag
    11461 gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa aggctggccg
    11521 caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac tcttgtcaat
    11581 agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg tgtattgtgt
    11641 attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt gtctgcgatt
    11701 tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga acagattgct
    11761 tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca taatatggct
    11821 tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag tactttcctt
    11881 gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg gccgtgggta
    11941 gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt gcgttacaac
    12001 aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg gtatatttca
    12061 tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta cttgctgaat
    12121 gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga acgtatgcaa
    12181 aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg agcgtgggac
    12241 aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg aatcagaagc
    12301 aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt cgtgaatttc
    12361 ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata gtaattcctt
    12421 cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct gataatattc
    12481 caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt tgtcgattgt
    12541 ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt cttttgagac
    12601 tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca atacttttaa
    12661 aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa aaccatagcg
    12721 acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt ggtttttcgc
    12781 aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct ttctcaacac
    12841 ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat atacgatgga
    12901 ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta catcctttac
    12961 taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt ttgactgccc
    13021 attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat ccgctatgta
    13081 aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta tatccggtaa
    13141 atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt gccatagtcc
    13201 ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg ccagatcaac
    13261 ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca ggtgatcggc
    13321 aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa cgatgtattg
    13381 cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt ttgttcgaaa
    13441 aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat aatgttcttc
    13501 cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc gctgtttttt
    13561 cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag aaattttgat
    13621 gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt gccattcttc
    13681 cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa cgttagcaac
    13741 cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa tatcttcttt
    13801 attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt ctaaagacat
    13861 agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca cgtggatgaa
    13921 cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat acatgatatc
    13981 attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat gatcttgtac
    14041 attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac cgttttcttc
    14101 taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt ctgctagatt
    14161 gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga cacagatcac
    14221 gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt tttcgaatac
    14281 ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca ttttttttac
    14341 tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg ttactagtac
    14401 tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt atcataccat
    14461 tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca gatcaaactt
    14521 aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt gcgacaatta
    14581 ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa tttggaaaac
    14641 tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt ctttcatctc
    14701 cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca gggtcccaca
    14761 aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat gactaaatac
    14821 gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat tccccatgat
    14881 taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca catttccttc
    14941 tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc ttgtaaatac
    15001 cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg tgcccagcat
    15061 atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga acatcaagat
    15121 tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat tggtaaatag
    15181 agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt tcattcctag
    15241 tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc ctttgctgac
    15301 tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca cgattgccgg
    15361 aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg ctgacccata
    15421 acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca atacgagtcc
    15481 catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag gatttgtaaa
    15541 aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc ttttctttaa
    15601 gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact tccactaaag
    15661 caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg actagcaacg
    15721 cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt tccatagctg
    15781 aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct tttgattcga
    15841 tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact ccaccaaaac
    15901 tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca atcataccag
    15961 cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga ccatttcttt
    16021 caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga ttgtttacaa
    16081 aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc aaagctgtaa
    16141 caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac ccgagaataa
    16201 caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca taaaatgaca
    16261 atatt
//
LOCUS       DAIGOK010000062        16153 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.062, whole genome shotgun sequence.
ACCESSION   DAIGOK010000062 DAIGOK010000000
VERSION     DAIGOK010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.062"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(60..557)
                     /locus_tag="NOA01_001586"
     CDS             complement(60..557)
                     /locus_tag="NOA01_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HCI0632782.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(687..1397)
                     /gene="budA"
                     /locus_tag="NOA01_001587"
     CDS             complement(687..1397)
                     /gene="budA"
                     /locus_tag="NOA01_001587"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCI0632783.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(1410..3074)
                     /gene="alsS"
                     /locus_tag="NOA01_001588"
     CDS             complement(1410..3074)
                     /gene="alsS"
                     /locus_tag="NOA01_001588"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HCI0632784.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(3279..3941)
                     /locus_tag="NOA01_001589"
     CDS             complement(3279..3941)
                     /locus_tag="NOA01_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HCI0632785.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            complement(3951..4766)
                     /locus_tag="NOA01_001590"
     CDS             complement(3951..4766)
                     /locus_tag="NOA01_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCI0632786.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(5028..5471)
                     /locus_tag="NOA01_001591"
     CDS             complement(5028..5471)
                     /locus_tag="NOA01_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HCI0632787.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(5605..5943)
                     /locus_tag="NOA01_001592"
     CDS             complement(5605..5943)
                     /locus_tag="NOA01_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCI0632788.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(5931..6308)
                     /locus_tag="NOA01_001593"
     CDS             complement(5931..6308)
                     /locus_tag="NOA01_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCI0632789.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            6532..7725
                     /locus_tag="NOA01_001594"
     CDS             6532..7725
                     /locus_tag="NOA01_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCI0632790.1"
                     /translation="MTTYQRKLLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(7891..8313)
                     /locus_tag="NOA01_001595"
     CDS             complement(7891..8313)
                     /locus_tag="NOA01_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCI0632791.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            8903..9358
                     /locus_tag="NOA01_001596"
     CDS             8903..9358
                     /locus_tag="NOA01_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632792.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQQAAAEQASQQAQAAAP
                     EQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSKCYGN"
     gene            complement(9520..10692)
                     /locus_tag="NOA01_001597"
     CDS             complement(9520..10692)
                     /locus_tag="NOA01_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HCI0632793.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(10898..12327)
                     /locus_tag="NOA01_001598"
                     /pseudo
     CDS             complement(10898..12327)
                     /locus_tag="NOA01_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306286.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            complement(12324..13826)
                     /locus_tag="NOA01_001599"
                     /pseudo
     CDS             complement(12324..13826)
                     /locus_tag="NOA01_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345213.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
     gene            complement(13823..16150)
                     /locus_tag="NOA01_001600"
     CDS             complement(13823..16150)
                     /locus_tag="NOA01_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCI0632794.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSATETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         4599 a         3231 c         2864 g         5459 t
ORIGIN      
        1 ctgtctttcc ggctcttttt attcttgatt tctcttcttt tttgtttcaa tagacgattt
       61 tagttattaa taaatgaaaa agcatttata ttaaaatttg ctaataaaag atctacatat
      121 ttttctagaa atgcttgatc gatttcatca taccctttcg ttactccgca atcgatatct
      181 aaaacaccga ttaaattacc ttcttttacc attggcacta cgatttctga ttgtgcggct
      241 gaatcgcagg aaatgtaatt tgcatgggtt ttgacatcat caacgatcaa tgtttcttgt
      301 ttttcagctg attctccaca aactcctttt cccatcttga tacgtgtgca agaaactcgc
      361 ccttggaacg gtcctaaaat caattcatta ccatcgaata aataatatcc tgcaaagaca
      421 gtctcaggca aagtctcatt caacaatgca gatgaatttg ctaaatttgc taaccagtta
      481 tcttctgctt ctaataatcc ttgttgctgt aataataaaa tttcgtatgc atcttttttc
      541 tgtttttctg tccacatgat aaaaaccgct cctttttgat ctatggagaa gattatagct
      601 cagttttttt gtaaacgcta ggtttcctcc tcaaaaaaac gagatacgtc aggttaatca
      661 ccttgaacgc atctcgctgt ctttttctat tctgcttgtt ggatttctgc tgctaaatca
      721 gcataatcaa tcgtttcgtg aacaaaagtc ggatcatcaa ctggaaaatg ctgtacaagg
      781 gacgagatgc ggctaatctc aacttttcct tcagccactt caaaatccat tacatgtccg
      841 ccaaatgtat gtgtatcatc gataaaatgc aagtgaaatc ccgctgcagc aactccttgg
      901 aataattcag gcgtaaagaa cccgactaat gttccttgga cattgctttg ttggaattct
      961 ggttgagact ctgaaatttt tgctagtctt ctgtaaggtt tttcttgctt cggcatgata
     1021 cggatgtgca tatttttgaa taatccagtt atcttcactg ccaaaaatag gttttccccg
     1081 gcttctctta ctaatatgtc ttttttcaat tgttctccat ctgcagtttt tgagacagaa
     1141 aaactagtat ctggcgtaaa atctgtaatt gctgcatagg gggtaagttc acttccatct
     1201 aattgggcaa ctgagccatc tgatcttcct tgataagcta cgccatctaa aaagatgact
     1261 tcaccatcaa gcccatgcaa tgttcccaat cccagagttc ctttttctaa tatatgagca
     1321 atcgtttctg ttccatccat caagccagcc atcaaagctc ctaatgtccc gtgctggtat
     1381 aagatttttt cagtcatgtc gacacttcct taatagaatt gatctggtaa gattgtttct
     1441 cccaatttct cattatcacg gtaatcaatc ggaatatcaa taattacggg tccttctgtc
     1501 gcaaatcctt cttctaaagc tttttctaat tcctctacac ttgtagctcg gattccttta
     1561 gcgccaaaag cttctgcata tttcacaaaa tcaactggac caaagtctac acctgacgcc
     1621 cgctgatatt tcattttttc ttggaactct accatattgt agtgtccatc attccaaatc
     1681 aaatgaacga tattcaattt tttacgtacc gccgtttcta aatcctgggc agagaataaa
     1741 aatcccccat cgccggaaac tgatacgatt tgtgtatttg gacgcaccaa tgcagcagaa
     1801 atcgcccatg gcaatgcaac accaagcgtt tgcataccat tactgaataa caaatgacga
     1861 ggttcataac ttctaaagtg gcgtgccatc caaatataat gactacctac atctacggta
     1921 acagtcatat catctgttac ttttgactga agcgtgttaa taacttctaa aggatgcaag
     1981 atacctgctt ctccttttga ttgaacaatg tcccgctccg ttaattctgc ttgaagagat
     2041 gctaaatatt cttttgcatc ttctgacact tgttgaggct ctaatgaacc tgtcaataaa
     2101 tcaagagtgg ctgagatatc accgatcaat tctctttcag gctgcataaa aggatcgatt
     2161 tcagctggcg cttcatcgat cacaatgatc cgcgcatctt tttccgcatt ccagtttctt
     2221 gcttcatatt cgattggatc atagccaata gcaataacta gatcacttcg tttcaaaagc
     2281 atatctcctg gttggtttct aaataaaccc acacgaccaa agaaatgcgc ttctaattca
     2341 cgtgagatca ctccagctgc ttggaatgtc tctacaacag gcaatgctgt ctttccaact
     2401 aattgcctga tggctaacgt ttctttttca cttgatcctc gcatacctac taatagtacc
     2461 ggtagttttg cttctctaat gcgttcagct aggtaacgga tatcatctgc agaagcagaa
     2521 cctaatttag gatcgctcaa tggcttgatg acattccctt ggacaggtgc atccactaca
     2581 tcttgcggga tactgataaa gctcgcgcct ttttttgaac tcttagccat acgataagca
     2641 ttggcaatca cttctgaaat tgtttctgga tcttggattt ctgcactgta ttttgtgatt
     2701 ggctggaaca gtgctgcatt atccatgctt tgatgggtca acttcaatag atcgcttcgt
     2761 tttacttgtc cggcaattgc taaaactggg tcaccttctg ctgtcgctgt taccaaacct
     2821 gtagctaagt tactagcacc aggaccactt gttgcgataa ctactcctgg ctctcctgta
     2881 atacgtccga ttgcttgtgc cataaatgca gcattttgtt cgtgacgagt cacgattaat
     2941 tcaggacctt gatcctctaa ttcattaaat acaccatcga tttttgcacc tggtataccg
     3001 aatacataat caacatgatg attaatcaaa ctttctacta cagatttcga accttgtttc
     3061 attttttcgt ccattgaaca tcgtcctttt tatgctttat ttttaattat tagttagaaa
     3121 taatcacaaa taattgatac agtttctaaa cctgtttcat tcaaaagtat agcacgtttt
     3181 atttcatttg tttagccata tgtttcaaaa aatgaaaaag aatgacagtt tttacataaa
     3241 aactgtcatt cttaattgaa agtttcaatt tataaagtct acagatccat agttctcatc
     3301 aatttacgat tgttgaagta aatcaaacca aatacgatag aaggacctag tagaatacac
     3361 cattggggct ttacgaaaaa gaagagaaac aaacttgtca atagtaaaaa atctagtaaa
     3421 ctcacatacc attttttcaa cgtatagtag aacgctatcc gtagaacttg atttggtttc
     3481 gacgctggat ttctcacctg aaaataccag tagttgatgc taaaggaaac tgataaacaa
     3541 gcaagtaaaa caaacaacgg aatcaaccat tttccataag aaaaacgtat aaaaaagaaa
     3601 caatcaaaaa tcattatcac actaactatc caagcaaata gccaataaaa aaatcccttt
     3661 agccaaaatt ggcgaaacgt ctttatgaac aaacgtactg gtgaaaaact tttttctttt
     3721 gctaattctt ctgtcatacc tatcatcacg atgcaacctg gaccaataaa aagtaaagaa
     3781 aaaataaagg aaaagacatt ccttccatct atcgacaaaa ggaacgcagc tgctatatac
     3841 aagatattga cacaccaaaa acaaaggcta cttgccaatg attgatacaa ccaataaaag
     3901 aattccatat aaacattttt gtcaaaacgc tgctctttca taatcacctc ctattccttc
     3961 attcctgatg aagcgatccc ttctacaaaa tatttttgca tcgctaaaaa tataaaagca
     4021 acaggaagaa cagaaacaac tgaggcagcc atgattaatg cgtactctgc atcaaatagc
     4081 ccaataaaca ttttcaaccc taactggatt gttttatttt ccgtagaaga caaataaatg
     4141 aatggcccca tataatcgtt ccatgtatta acgaatgtga tgatcgtcaa actggcaatc
     4201 gccggttttg taagaggtaa aatgatcttg cgatagattc cccactctga taagccatcg
     4261 atccgcgcac tctcacataa agaatctggg atactactgt agtattgctt caacaagaac
     4321 acaccaaaag cattgaacgc ttgtaatagc accaacgacc acaacgtata tgttaaaccc
     4381 acttttctca tcataatgaa ctgcgggatc atataagact gccaagggac agcaatcgtg
     4441 ccgatatatg ctaaaaataa aatatcgcgc cctttaaaat ttagtttaga aaacccgtaa
     4501 gcagcaaaac tagaagtaaa caactgaatc aacgtgatca caacgcttaa aaaagctgta
     4561 ttcttaaaaa atgtcactaa aggaatacga gaccaaataa ccagataatt ctctaaatgc
     4621 caagttttag ggatccattg aatcggaatc gtgaacactt cattatttgc tttgaatgaa
     4681 gcagaaacca tccataaaaa aggaatgatc gtcaataaag ctaatacaat cagcagaatc
     4741 atacccaacc attgaataaa gggcatgttt tttgaacgaa tttttgcttt ttttctttca
     4801 tttcgaacac ctccaaagtt ataatcttca tttgtttgta atgattacaa tccagaacaa
     4861 cggttattat agctgattca tttccgttct tcttatgttt gtaagcgctt atttaacaag
     4921 ttatgtaaat ctctttattt tttttgattt aaaaatgaaa aaatacaaaa gcaagactgt
     4981 catacgtctc gcttttgtat tgaaatttct tcgattagag atacttatta ctgttcaaaa
     5041 gcttctttga aacggtgaat caattcattg actacaggtg ataaaatccg attctttttc
     5101 cagactagca tgcagttcgt ttcaatcatt gggtcaagcg gaacgaatgt cactcgcttt
     5161 gaaagatgac ttacagcacc ttctatgact aaagcagcac ctacctgatt ctcaattagc
     5221 gcaaatacat tgaaaatcaa attgtacgtt cctgtgatca gcaactgctc cttttttatg
     5281 cctaaccagt cagcaaacat tcgctgtatt tctggacgct ttgagatcaa aagaggtaca
     5341 ttcgcaaagt cttcttttgt tatcacgtct ttttttgcta aaacagaatt ttctaaaatg
     5401 agcaatcccc atttttcctt tcttggtaac aaaatcgttt cgtatttctc tgtatctact
     5461 ggatctagca aaatcgctaa atctaacagg cttttttcta gtcactccgt gatatcatca
     5521 cttgttccgc tgtggatatg aaattgaacg ctgggataat cacggatcaa cttttctagt
     5581 aatagtgcca atgtattcga attatcagct tccacacagc cgatcgtaaa tcggccgctg
     5641 aataattgtt tttttctgtt ctcgaattct tgagtggttt gattcgttaa atctaaaatt
     5701 tcttctgctc ggctttttta gaaaataacc tgcttctgtc aagatcattt tgcggttttc
     5761 gcgtacaaac agttctgttc ctagttcctc ttctaactcc ttcagttgtc ggcttaaggt
     5821 tggttgtgta agatgtagaa cttctgcggc ttgagagatt gttccttctt ctgcaattgt
     5881 ccaaaaatat tttagtaagc gtaactccat agcgatgctc cttgattttt ttacttgata
     5941 cattccattt tatttctaga taacacacga tcatatttct tgatttttcc ttgtaaacgt
     6001 tccaaacatt cttgcatttc agcaattttt ttcgtcaaaa gttcactttc ttcaatcaaa
     6061 agcttacgcc gctcaaatac agttgcttct ccttcgctag ataggcgaat atattcaatc
     6121 aaagactcga tcgataatcc ggcatttcgc atacagatcg taaagtcgac ccattttagg
     6181 tcttcttctg tataatcacg gattccgcct tttgtccgat taactggtgg aatcaacccg
     6241 attcgttcat aatagcgcaa tgtatcggcc gaaatgccct tttcttcact gacttgtttg
     6301 atattcatct gatcactctt tctcttaatt tcttgccact ttacttatgt attttattta
     6361 ttctacaact ttaagtgtac tccaagtcaa tactcatttc tattaaaact aagctggatt
     6421 atctatatcg gttgtttgtg tattatttca aataaaaaag tcttgttttt ttattctttt
     6481 ttggatatac ttttaacaat ttagggaaat tttaggaaaa ggggtataaa aatgacaacg
     6541 tatcagcgaa agttattatt atcgacatcg gcgggaattg ctttagagaa tatggatatt
     6601 atgtttttag ctttttcatt atcttcaatg attacttctt ttcatattag tggtacgcaa
     6661 gcgggtttca tttcaacgat cactaatttg gggatgctca ttggaggtat tttttttggc
     6721 atcatagctg ataaatatgg acgtgtgaaa gtattttctc aaacggtcct tctgttttcg
     6781 attgcttctt tattgatgta ttttgcttca aacatctact tggtttatct ctttcgcttt
     6841 atcgcaggtg taggagcggg aggagaatac ggggcgtgta tgtccttgat ttccgaaagt
     6901 ttttcaaaaa aacaaattgg acgagcttct tcggttgctg gtattggtgc gcaggtcggt
     6961 gcggctttgg ctgcgatttt agctgctgtg atcattccaa cgtttggttg gaagatgttg
     7021 tatgtcgtag gtgtactgcc ggtgttgatg gtcttgttca ttcgtcgcgg cttgactgaa
     7081 ccaaaggaat tcaaatctgt acaatctgcc gaaaagaaaa ccaaacttag cagactgttt
     7141 gaatcaaaac atcttgcttg gcaaacatgt ggccttagta tcatggtaac tgtccaaatt
     7201 gccggctatt ttgggttaat gaattggctg ccttcaatta tgcaaaatcg attagggctt
     7261 tcggtttctg ggtcttcatt gtggatggtc agtactattt taggcatgtc tttgggaatg
     7321 atgacttttg gcataatcat ggataaattg gggcctaaac tagccttttc tttgttttta
     7381 atttgttcat ccttatctgt ctatctactt gtgttagccc atagccagtg gagtttggta
     7441 gcagccgctg tgatagtcgg ctactttatc aatggcatgt atggtggata tggcgcagtg
     7501 attagtagtt tgtatccaac agaaatccgc gcgacggcga ataatttcat tatgaacctt
     7561 ggacgagctg ttggtggttt ttcttctatc gtcatcggtt tcttgatgga tcattatagt
     7621 ttaactgccg taatcttgtt tttgagcggt atttacctag tcagtctgct ggtcttgaca
     7681 actcttactg gtgttacagc attaagggaa agcttgcagc attgatagtt gagtctgtaa
     7741 atgacaagaa agaggtgtgg caaaagcttg catctgcggt aataagacca gccaaatcaa
     7801 aaacatgaga cactgtttcc gtttggctgg tcgtattact tgatgcaggg cagctttttc
     7861 aactgctaat ttccttctat ctggcggcta ctattctttg acgtccgtca tttcaaagta
     7921 ctttactaat aacttagcat aacgaaaaac tgtggataca ctcgtatatt gagcgtctgc
     7981 ataatcttcc aaatttttct caggatgttg atacatgaat tcaatcaaat gataattata
     8041 tgattttctg tagaaatgaa gataaataat gtagattggg aagttctcct gcttttccat
     8101 agaaaagcgc cgattattct cattcttcaa aatgacctta tcaggatatt tttggaacaa
     8161 ttcttctaaa tctgttagat aattcaagat agacttactt gataccttca attcgttttg
     8221 tagttcttct attgaacacc aacgttctgt ttcaagcagt aaatgaatca ttttaatttt
     8281 cttttgagaa acttttgtta aaaaagtctg catattttct cctcctgtat ctactttact
     8341 atactacata caagaaaaaa aacggataca aatatcatat tgtcacaccg tcaatagtgc
     8401 ataaacctgt tattatttct ctttttttcc taagtattct attgatatga atcatttaat
     8461 cgtttattat ataaatgtca ataatctaat aataaaataa cgtacatatt ttattattgc
     8521 atacttaata acagaactat agcaaaaaat cccgtgtatc aaaacgggat tagattaagt
     8581 ttcctcaatc actattcata acacaattat tataacaagt aagtaaacaa ttgaaaagga
     8641 catttattga aaacggttgt tttttactta aggaggaaat ttagtgaaaa aattagttgc
     8701 attgtttttg gtcgcaggaa gtttgttgag tgggtgtggt gctactacgt ctactactca
     8761 aagcacaagt tcagctcagg cttccagcag cagtaccacg atctccgaaa gtacggcaga
     8821 ttcatcgaaa gaacaagaac aaattgaaaa gaaaaaagaa gaagagaaac aaaaagcgga
     8881 aaaagctgca ctagaaaaaa aagtgacaga agcggaatct gctttgtcac aagctgaagc
     8941 aaatcctaca aaagaaaact atgcaactgc ttcagcagct atccaagcgc tacctgaaaa
     9001 caaacaagag ttgacaagcc gtcttgctgc aattgattcc acaatcaaag caaaagaagc
     9061 agaagaagaa cggaaaaagc aagaagaaat tgcacgacaa caagcagaac aagcgcaaca
     9121 agaagcaatc gcacagcaac aagcagaaca ggcgcgacag caagcagcag ctgaacaggc
     9181 ctctcagcag gcacaagccg cagctcctga gcaaaataat gaacaaactg tctatgtcac
     9241 accgaccggt tcaaaatacc atacacataa atgtggaaat ggtacgtata cccctgccac
     9301 tttaagcgaa gctcaagctc gtggtttaac cccttgctcc aaatgttatg gaaactaatt
     9361 caaaagttat actataataa taaaattctt ctgaaacata aaaaaataga agctggcgtt
     9421 ccagatagta aaccaaaaat agcagtttac acgtccattt cgtcagcttc tctctttttt
     9481 catttagtct gttccctctg cttttttgga catcttcatt cattctttct caatcaaata
     9541 gtacacctct ttttcttttc gcagtttata tcttcctttt ttcaatttca ttgaaaactg
     9601 tttcgaatgt ttatgtttta catggatttt aagcaggagc tcctcatgtt gcaagcgata
     9661 gttcctgcct tttaaatcag aaaatctcac tagtccgtat tcatcagaag ctttcacaaa
     9721 tggaactttt tctctagtaa tatagtgttt gccgttgagc gtttttacag aaaagaccat
     9781 tccttcgatt ggtcgtccgt ttgtgttctt tacataaaaa gataaaggat agtttcgctt
     9841 agcgatggcc aaagaatcta aataagcgat gagtagccaa actaagccga ttgatccaag
     9901 tagtataccg ataaaccaaa taaaaaattt ccattttttc cattgatcaa ttccttggat
     9961 cgcttttttt gcctcttttg cttgataagg aatacgatgc cccgtcacaa gcagtcgatg
    10021 tgtattgacc atataaggag tacaggtaag caaagtcacg agatctctgc ctttttggat
    10081 acccaaagaa tctacttcat caggtaagac cacttggatt ttttctactt gatacgccaa
    10141 tgtctttcca ttgatctgga tgaaaaactg gtctcctttt ttcaacttag gcaaatctgt
    10201 aaataatttt gcttctggga gtccacgatg cccagaaaga acggcatggg tgtttttccc
    10261 gccagtcgga taagaacttt cttccaataa tgacgttcct ttttgcaaaa atatttccgt
    10321 tgtctgatcg aatataggta agctgacatt gatttttggg atttcgatga ctccaagcat
    10381 atgttccata aaaaaggcat tactaggtaa atcttttaat gcttttgcat caactgcttg
    10441 gttgaaactg cctatccctg gtgatacgtt ttgctctgcc aactgttggt ttttctttgt
    10501 cattttttcc tgctgttttt ttatttcctc tgaattctta ttcgatgctt gcttttgata
    10561 atggatgatc atttgctggg caagaaaatc attcagtgaa tcttctacaa aaggataaga
    10621 aaagatcccg atcccaacga ctaaaatgat tgccatgaag atgtccagta ttcttattct
    10681 gcttttattc atcatgtcca cctattttct aatatgtata tgaagaggtt gtgaaatcag
    10741 cgccttgacc tgagtattcg ctcaacaaac ggcaaaatag ttcctcctat tttgccgttt
    10801 gttgagcgaa taccaagaga ccgaccactt gtcacaacct ttctccccat ttgaatcact
    10861 atcgtttatt tatcgtatga tttttgtttc caatagttta agcttctgtt tcttgatggc
    10921 gttttttgaa gtagatcact gctcctccaa ctaaagcagt acctaccaag atgaatacga
    10981 tgatccccat accaccagtt gaaggcaaga ttcctttagg ttggttatcg atcgttgttt
    11041 catcagaagt tgttggatca tcatctactg taaattcagt gtacgcagct tcattcttag
    11101 gtaatgcgta gccttctggt gcttttgttt cttgaaggca gtatgcccct tgtttcagcc
    11161 cttcgattga aaatttccct tctgaatcag aaatcaattt cacttcttcc cagtcagaac
    11221 ctgtgacttc atctgaccaa gcatatgggt attctcctga ggcaagattt gctatttttt
    11281 tcattaattt aacagttgga tcttttgctc ttagaataga aaattctgca tcggctaatt
    11341 tttcttgcgt gttcacatct tgtttgatga agtggaaatc accgtagttt tggacatttt
    11401 ctacagtaat cgtgttatcc gtagttgtat tttcccgatt gactacaccg tttggattcc
    11461 caagattggc ttcatctgcg ccatggatga taccggttgt ttgttgcaat tttccgtttt
    11521 ttactataaa agtagtttct ttttgtggat tggttaattg ataaccatct ggtgcttttg
    11581 tttcaactaa agtataattg ccgtctatta atccattgac actgatggat ccttcagtat
    11641 cagttgtgaa tgtcaacgct gactcagcag gatcatagag agcgacacca gtgttttcat
    11701 caaaaccggt cacgaattga ttctctgaat ttttgatctg gaattgcgcc cctgctaact
    11761 tatctacccc tgaaaagttc ccagctttct ggattttgat attgctttct ttgatgacat
    11821 ttttagcata taggtgaatc gtgtctaaag gtttgtcagg gtctgatgaa ttcgggttat
    11881 aaaagggtaa gcttaacacg atatttggcg acgtttcgac accgggcttt ggtgtttcta
    11941 caaagacgta tacagcatct ttcccatcag atttacctgg aagagtcagg ttagctaatc
    12001 cttcaccaga agtcgttaca cttgcgaagg gtgtagcgtc tgctggaact gctgtgtctt
    12061 tacttggata gtacgtagca gtgatgtcat atacgttgaa ttcaacgccg ttcaacggtt
    12121 cgctattcgg aaatgtcaat tccgttccgt tgtttatttg attttctggt gctgaagtga
    12181 atttcaattt atgcaaatta accgtaacat tttctggtgt aggtgatacg gcctagaaac
    12241 ctctgcagtt cctaggccga gaacgaaagg aagcagcatg atagcagttc ctaaaaacgt
    12301 ccatattttt gttttgatgt ttttcatttt atttccctcc tattgtttcc tttttgttgt
    12361 tttttataaa accaagctgt ccctgttgaa aggactagca acaaccctaa aattgagaaa
    12421 cctccaagca tctcttcgct tgtttttgga aagcttcctc catttttttc atttgatgga
    12481 ccatttgtct gcatctgaac tggcgttttt ccattattcg tcggatttgc ttttggttcg
    12541 ttgatgattt ccaatggtat aggatgaagc ggatcataac tgcctttttc aacttcaaag
    12601 agaatggttg attgactcaa tatatatcct ttaggcggtt taatctccac tagttcgtaa
    12661 cttccaaaag acaatccatg gacagaaaat gttccatctt tactggaagt aaaaatcgtt
    12721 gctcttgaat catctttggc tacccaatga tttatcccgt tttcttgcgc taagtattgg
    12781 tcattttttt tgactagaaa ttgagcatct gctagttttt gtttcgtttg ccgatccact
    12841 ttgacaaatt gtttgcctcc tgtttggatg gcaagatgtg tttctactgt ttgattgtca
    12901 gtcgtcaaac gtacatcgtt atcaaatgtc gttttttcac tgttttcttt caatgtcatt
    12961 tggtaacgga tcgttaattt tttcccagca aacgaactaa gatcttttcc agaatctgat
    13021 aagacaaaac catgttttgt tttttgtgtg tgaaatgtat gaatcttgtc tccaccaatc
    13081 aaaaaatcga tcgtttctgg ttctagccat aactgaggat cagctgtatc tttgatcgta
    13141 taggttccta tctcgttgat gttctcagga attgttacgg tgatgagata aggaacttta
    13201 tccccatctg caaaatctgt ctgcttattc tctactgttt ttgttaatgt gagtgcagca
    13261 ggtatttttt gttcgctctt tggatacaag tggatggtcg tcaatttatg attagatgaa
    13321 tctgtcacag gaagcacaac gacaaatggt gctgattttt cttttactta atctgaagtc
    13381 tttatttccg caaatcggta aacagcgtca cgaccttgct catctttgtc cgatgcacta
    13441 aaagaagcaa tgccctcttc ctcgtctact gtttttgtca cattttctgc cagtattttt
    13501 tcactatcac ttttttgtgc tagttttctt tgtgcgtctt ccacactact tccttctgac
    13561 cgcagcttgt aaaattcatc cgagacatca tatatagcga aagtaacgcc gttcaaccct
    13621 tttactcctt gaaagttatc cgggatattt attccgtcgt tttgactgat acttggcatc
    13681 ttatcgcttt cgaacattcg attatgcaaa gtaaatgtaa taggtgattc tctagctgac
    13741 acagtcggag aacctataaa aaagaccagt aggataaaaa agagaacagc aaatatatcc
    13801 acacgtacat tttttgtttt tttcatcgct gttcacctgc ttttttctta cgatggataa
    13861 aataaaagaa gccagaaaaa ataaagatca gtcctaaaaa gacaaagggc agtagaccta
    13921 tcccacctgt cgaaggaagc aagagagtcg gattgatcgt caacttagcg gtatctgttg
    13981 cgataatcgt ataattattg tttacacttc cttcgcctaa actaatcgtt actatttgtg
    14041 atccaatatc tgtccggctg ttaactgtta gggtataatc caatgattcg cctaagacaa
    14101 caccggtaac gtggagattt ccaaccgaaa tcgagtgact ttgggcatca taattaaagg
    14161 cagcgtcatc gacagtaatc actgcgttag ctttatagat ggtataagtt cctgatgtgt
    14221 tggctatatc agtaaatgac aacttgggat ttgctgcttt gactttgtct attccgctat
    14281 tcgttagttt aattgtatag ctgccagcat taaccatatt ttttgctgat tgtgcatctg
    14341 ctgtgtaatc catactgcta agtgccactt gtactggagt aagcgaacta gtgacaccct
    14401 ctgctagctt gacatggatt gacgggacac ctgcttttcc atcatatgtc ttactgccac
    14461 tgagctctac cgcaccgctg atttgcttaa tcgtatagac atcagatgtc tgcacgatag
    14521 taaataaata gtttgttctg gcagctagtt tagctagtcc gctttttgtc aattgccaag
    14581 acgtataggt tccaatttca gtaggcgcag ttacttcaac tcccttttca ttcatcgctt
    14641 tgaaatcagt tggattccat gtgatttgat caatactgag atcattgttt aaaagggatg
    14701 ttgattctgt tttatcatta aaggcatatt taacgatcgg tacaacagaa agaccatcat
    14761 agtacttact gccatttgca ttgattgata ctgttgcatt tgtcttataa tataggatga
    14821 cttcagaaat ctgttttcca tttgcgtcct ttacgattac atttccattt gtatctaaac
    14881 tagctaaaac actcattgct gattggttct ttgtttctgt cgctgaaaca gctgtaaagg
    14941 tatatccgtt gatagtaggc gtattagtcg tcaggtcttg tgcagcactt ccgtaagtaa
    15001 gattcattat tttagaagga gcaatcatct gtccaaaata gtcttgatac ttaatagtga
    15061 ttggtgtttg tttgcctgta taggtcaccg tatcgacgtt actttctagg gctgtgaaag
    15121 aaccgtttgt ttgtgcaaaa gaatagcccg ttactgctgt tggagtgata accactttgt
    15181 cacccacatg gattccagat aatgtagtcc cgtacgttcc gatttttgct gctccaccat
    15241 tgactgtata agaaaaagtt cctgtccgat tcgcattgac ctgaaccgtt gctgcttgat
    15301 aggttggaaa atttgaaaca tttcccttac catcaacgat ctgcccattt ttgatcgagt
    15361 accccgtgaa tggaatatca tttgctaact gtataccgat tgaaaaagta ctcatacctc
    15421 ctccggcagg aagccctccc catgttgagg gtgtaaaaga ataactttta tccgcattgg
    15481 gtgtaactga atcgatcgta tttccagcgg tagccacccc catatagata ctttttggga
    15541 atgtgatagt agtaccattt actgtggagc caataaacag attatggata tctggacggt
    15601 tatcaataaa agcgattgct ttggttacgc cttgtaacgc agttttatcc gcactcatcg
    15661 atccgttaac cgcaacaatg actccatctt tggcatatat cactgtatat ccatctggta
    15721 tggtatgtcc gttgaaattc gcagtggccg caacagtact aaatgtctgg cttctacctg
    15781 tttctgaata agctttctcc cctgctgctt ttgctgctgt gatttctttt tctgtcggat
    15841 caatcagttg agtcgaggat gtggttgagg ttgtctcgtt gttgctagac tgattggtgc
    15901 tttctatgga ctgtatttca gaatagggtg gactatttgt ctcctctgtc gtttggttag
    15961 caaaatttga cgtttctgta taaacgttta cataaatact tgataagctc cctaagaaag
    16021 taaaaatcaa gatgatcgaa atcataatgg gatgtaagta ttttctttta gatacagctt
    16081 tgagtctaga aacaaaatgt ttttgacata tacgttggat tttattttga gttttcttaa
    16141 tcattttcat ccc
//
LOCUS       DAIGOK010000063        15818 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.063, whole genome shotgun sequence.
ACCESSION   DAIGOK010000063 DAIGOK010000000
VERSION     DAIGOK010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.063"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(51..353)
                     /locus_tag="NOA01_001601"
     CDS             complement(51..353)
                     /locus_tag="NOA01_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632795.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(573..939)
                     /gene="ssrA"
                     /locus_tag="NOA01_001602"
     tmRNA           complement(573..939)
                     /gene="ssrA"
                     /locus_tag="NOA01_001602"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1110..1883)
                     /locus_tag="NOA01_001603"
     CDS             complement(1110..1883)
                     /locus_tag="NOA01_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCI0632796.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMSNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2027..2377
                     /locus_tag="NOA01_001604"
     CDS             2027..2377
                     /locus_tag="NOA01_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HCI0632797.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2358..3074
                     /locus_tag="NOA01_001605"
     CDS             2358..3074
                     /locus_tag="NOA01_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCI0632798.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3074..4522
                     /locus_tag="NOA01_001606"
     CDS             3074..4522
                     /locus_tag="NOA01_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCI0632799.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4592..5101)
                     /locus_tag="NOA01_001607"
     CDS             complement(4592..5101)
                     /locus_tag="NOA01_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632800.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5091..5816)
                     /locus_tag="NOA01_001608"
     CDS             complement(5091..5816)
                     /locus_tag="NOA01_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632801.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(6039..8159)
                     /locus_tag="NOA01_001609"
     CDS             complement(6039..8159)
                     /locus_tag="NOA01_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCI0632802.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYISAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            8389..9567
                     /locus_tag="NOA01_001610"
     CDS             8389..9567
                     /locus_tag="NOA01_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HCI0632803.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            9583..10341
                     /gene="aroD"
                     /locus_tag="NOA01_001611"
     CDS             9583..10341
                     /gene="aroD"
                     /locus_tag="NOA01_001611"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCI0632804.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            10364..10849
                     /gene="ybaK"
                     /locus_tag="NOA01_001612"
     CDS             10364..10849
                     /gene="ybaK"
                     /locus_tag="NOA01_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HCI0632805.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(10920..12173)
                     /locus_tag="NOA01_001613"
     CDS             complement(10920..12173)
                     /locus_tag="NOA01_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCI0632806.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            12290..13639
                     /gene="gdhA"
                     /locus_tag="NOA01_001614"
     CDS             12290..13639
                     /gene="gdhA"
                     /locus_tag="NOA01_001614"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCI0632807.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            13751..15097
                     /locus_tag="NOA01_001615"
     CDS             13751..15097
                     /locus_tag="NOA01_001615"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCI0632808.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(15405..15791)
                     /locus_tag="NOA01_001616"
     CDS             complement(15405..15791)
                     /locus_tag="NOA01_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCI0632809.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         5173 a         2828 c         3179 g         4638 t
ORIGIN      
        1 aagtatggtg ataaaatccg ttccgggcta ccttttttag gtagcctact ttaatcttta
       61 tacctttctg gatcaacgaa agtatacttc aaatagtcat aacgccgatg atcgctccgt
      121 gcgtctggca catcagtcac gatatcaaac aaaaaataca catctttctt cattctggtt
      181 tttgttgccg gaattttaaa gtagttctta ttagagtaat agagattgat taataagcta
      241 tcttcgattg ctaaaaagaa aacttctgaa tcccacacct tataaaaatc tttgacaaat
      301 ctattcgaag gatcaaattt aaaccataat tgtgtttttc cttccatcaa cataatgttc
      361 acctcaaaaa gagtatacga actaatgttc tttttgtaaa gtaggaatat cattgataat
      421 atggatggaa catatatatt aaaaagtgtt ctttttgaat gctattttga atgccatttt
      481 cattcattta acgaaaatct gcgaaaataa aaaatgctca aacccttata aaataaggat
      541 ttaagcatct agcgaaatct gctgaaatct cacaatggag acggcgggag tcgaacccgc
      601 gtccaaacac atcgccactt aaaaatctac gttcatagtc aactcattta aggattcact
      661 ttacagcttg ccgagtgaca ggcattctgt atcgctagtc tgatgttctc tttttcaact
      721 tacagacgga aagttgaaac gtatcccact aaagttagga cccttactcg agcacatggg
      781 cgatgccgag aggatctacg ctaactgttt ttaggcagct aaagcgtaag agttgttttc
      841 gtttttagca gttatattta actgtaacgt ttttacgtag acgtaaccta cgaaacgcaa
      901 ttcaagctcg actgtgcctg tcgaatccgt aacgtccccg ttagctaaca agggttacgt
      961 gtatttttca tacaaaatta gtatagcata tattgggata aattcaataa aaaagcgaat
     1021 catatcccat tatttatata ataactgccc ctttcttaag aaaaaataag cgattttctt
     1081 aagaaaagag gtagcttcaa aaaaagatct taaatcagat caaaatactt caatgcgtgg
     1141 acaattccgc cttctgtatt tttctttgtg ataaaggtag aaagttcttt taatccgtca
     1201 taggcgttgc tcatagcgat tttgttgtcg caagcttcca acaaggcaaa atcattcgga
     1261 ccatcgccaa atccaaaagt aggaactttt tccagctgaa gttctttttg cagacgtttg
     1321 acacctgccc cttttgagat tcctttcttc actgtatcga tcgagtaagg accattccga
     1381 taaaaagtta gctcaggaaa atgttcgtga tagtattcgt cacctgattg agataagatc
     1441 agcaacatat ttactgtgtt ttctttaaag cctaaaggat caatttccgg aacatctgaa
     1501 tgaatgaagt cgtaagcatt gatcattgtt tggctatgac cggtacacca gattttctga
     1561 tcgttatagt aagaaagttc atgaccgttc aactgtacat gatcatacat tttttgacat
     1621 tcttctgaag aaaatgtttg agaatagatt tcttcgccgt ctactcgaat gaaagaacca
     1681 ttcatggtga ccgcagaagt aatgcccgca tcttccatga tagagtggat ttctgcttct
     1741 gtacgtcctg tagcgatgac ggggagaaca ttgttttctt ttaatgcgtt catagctttt
     1801 gccacttcgg gtgtgatttt tgatttcgca tctagtaatg ttccatcaag atcaaagaaa
     1861 gtgattgcac gataatttga cacaaataaa actccttttt tattggaata agtttatctt
     1921 aacaaagaat gaacaaaaga caaacctttc gtgaagaatt cctgaacata ttggttcagt
     1981 caacaattta gagtatacta taagaagaga atttaggagg gaaagaatga tttttttcag
     2041 tattggtatc ttattcttaa taattggcag tctttttcgg attctgccct caaaaggaaa
     2101 tctgcctttt tatgggtatc attcgcctct tgccgcaaaa acagacgctc attggcggct
     2161 ggctcaaaag acaagcggaa actggttttt tcttatgggc ttactgatgg cattgatcgg
     2221 ctattattta aagacatctg ggcatacgaa ttattttctg attgaaatgc ttctgctagt
     2281 atttccaatt atgcctattt ttattatgac agaaaagaaa ttacagaaat atgacctgga
     2341 aactggaggg aacgataatg aatattttaa tgattgaaga taatcaatct gtttctgaaa
     2401 tgatgcagat gtttttctta aatgaaggtt gggaagcaac tttcaagtat gatggaaaag
     2461 aagggctcga tgccttttta gaaaacccac aacattggga tatgattaca ctagacctga
     2521 atttgcccac actggacggt atgctagtag gaagagagat ccgcaaagtt tcaagtactg
     2581 tgccgattat catgctgaca gctagagatt ctgaaagcga tcaggtgatc ggcttagaga
     2641 tgggggcaga tgattatgtc acaaaacctt tcagtcctct gacgcttatt gctcgaatga
     2701 aagctttgca tcgccgtagt gagttggcag aagcaagaga agaagcagta gtttcagatg
     2761 atcattttga tatcgagact agtcatttca agatgaatac gaaaacacga gaagcgtatc
     2821 tggacaaccg gccaattgaa ggactgacgc ctaaagagtt cgatctattg tttactttag
     2881 caaaaaaacc acgtcaagta ttctctcggg agcagttgct tgaattagtc tgggattatc
     2941 agtactttgg tgatgaacgg acagtggacg cccatatcaa gaaattacga caaaaaatcg
     3001 aaaaggtcgg accacaagtc atccaaactg tttggggagt cggctacaaa ttcgatgatt
     3061 ctggtgttgc ctaatgcgtt atttgtatca gcaactctta gctttctgcg gaatggtcgc
     3121 gttgatctta ttgatcgttg gagtttcttt tacacaactg acaaaacaaa cgattgaaga
     3181 aaacaattac cagcaactgt ttggatatgc tgaatcagtt gaaaagacaa cgcaaaccta
     3241 tgccgatacg ctcccgcagt ttagtcaaga tcaggcgttt caaaatgcat tgctgctgac
     3301 agagcaagta ctgacagaac aaaatgtgaa ctttatattt atcgataaaa acgaacgtgt
     3361 catttatccg actaatacag ctttactgaa ttttacctta acagctgatc aatggaaaaa
     3421 tctgaggaat ggacgtcaag aaaaattcac atcgaataaa aatattttag gacaagcaca
     3481 ggcgacctct tacgcacttg tgccatttaa tttgaatcat gaattctatg gagcacttgt
     3541 agttagtcaa cctgcgcgaa atatcgataa cagcgttcgt ccaattacac tgaacctgtt
     3601 caaaggattt attttttcca gtatcgttgc agttattgcc agctatgctt ttgcagcatt
     3661 ccaagttaag cgtatcaatc ggttaaggaa tgcgacgaaa gaagtgacga atggtaattt
     3721 tgatgtacag ctccccgtac atgacaaaga tgaatttgat gagttagcag atgatttcaa
     3781 taaaatgacg aattcattga aagaatctca agcagaaatt gaagaacaag aaaatcgtcg
     3841 aagacaattt atggcagatg cttctcacga gatgagaaca cctcttacga caatcaacgg
     3901 acttctggaa gggttagaat acaatgcaat ccctgaaaat cagcgagaaa acgcgattaa
     3961 attgatgaaa aatgaaacag agcgtctgat ccgtttggtg aatgaaaact tggattatga
     4021 aaaaatccgt acgaatcaaa tcagtatggt cattaaaaaa ttcaatggaa cagaaacatt
     4081 gaaaaatatt gttgcacagt tagaagctaa agcagaagca gcaggagata cattgacatt
     4141 gaaagccgtt gatgatattg atgtttacgc tgattatgac cgatttgtcc aaatcatggt
     4201 caatattatt caaaacgcga tacagtttac tgaaaacggc caaatcacga ttactttaga
     4261 aaaaggctat cttgaaacga tcatcacgat tgaagatacg ggaattggaa tgtctgaaca
     4321 acaaatgaaa agtatctggg atcgttatta taaagtagat ccttcaagaa aaaatacaaa
     4381 atatggtgaa tcaggattag gtttaccaat cgtgcagcaa cttgtacgct tgcataaagg
     4441 aaaattagaa gtagaaagcg aactaggaaa aggaacaaaa ttcgctgttc gtttgcctga
     4501 cgtagaaatc aaaaaagagt aaaaaacaag cggtataaaa gatcatgaat cgatcttcta
     4561 taccgcttgt ttttactata aaatgattga tttagcccgc gggctctaaa cgatcaatat
     4621 aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga ttttcttcat
     4681 gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta agttcatcaa
     4741 atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt aattgcagct
     4801 ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa ggagagatat
     4861 caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt acatccaaca
     4921 ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg atgatcgcaa
     4981 aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt ttatgctgtt
     5041 tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat tcaggtttca
     5101 ttacgtccag gcctcctact ttgcaaatga tggataaaaa aagtgaaagg gaatagacta
     5161 agaatgagac agcatccact gagcccaata agagtttggg caaatccctt aagtatatct
     5221 gtgaaaaaaa gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc catcggaatt
     5281 gtaagtccgc ttaccgaagt caagatatca cggatatctg gcagttcagt agtctttaag
     5341 acaagaaaat tgatcctttg aagaagcgct tcataaactg actggaagct tacgatccac
     5401 aataaaacca agatacaaca aagaaagaga ggaaagaaca gctgccagaa aataaaaagg
     5461 aataatttgg ttttagaaag accagtcagg tgccaattga tcaattcctg cttgttttgc
     5521 ctgactgaat agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa taaactgaat
     5581 atgcctttta tataaaatcg gctagctaat tctaaagccg gaagaagctc tgtagatgca
     5641 gaagcgccaa taaatgaaag ccgatcttgg acttgattgg ctaaagccga ttgcaaatgg
     5701 taaagagtca ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa acaatagagt
     5761 attgtctgct ttttgtaata aattaatcga gcagtagcat agcggattat tttcatactt
     5821 ttcacctcta taaaaagtat agaaaatgaa tatgaataaa gtatgaaatt cctttagtga
     5881 aatcacgaat taaaaccaga aaatattttg taaaaagcaa gaggttgtga agaagctgcc
     5941 ctattctatt accgtagatg caagcttttg aacactgttt attcgaaaag aggagcagca
     6001 acaataattt tgtcgctgct ccttttaaat ttattttatc attcggaaga acttgtggtc
     6061 gttccgtgaa gttcttggta cgtttgcgtt ttataaagat caacagttga ttgtccgttg
     6121 tgttcactaa agacactagt tgacttgtca ccaagagatt tttcagcttc ttttaatttc
     6181 tctaagccat tcttgtagtc ataatcactg gtatcaattg ggtctaatcc actattcgta
     6241 tagaaccgaa gaagatcacc attattgatt tggtcagaaa cagctaactg tttatcaact
     6301 ttttctttca gtgaatcaat ttcattctgt acttcctcag taggttcagt aatagcttca
     6361 ccagtttcgt tactatagat cgtaccaccg tagtaagtat attttggtgt aacaaaatca
     6421 ccatcacgga aagaaacaag ctgttcatga tctttcgaga atagatcttg tcctaactga
     6481 atataatttt ttgtatctac acctagtaag tgaagtaatg taggcaaggc atcaacttgt
     6541 ccgccgtaag tgtgattgat tccgccctgt gtttgtccag ggatatggat catataaggg
     6601 acacgctgca tttgtgcatt gtcgtagttt gtccaagtag aacttgtttt tccaactaat
     6661 tcagctagat ttttatttct tgaattcgag acaccgtaat ggtcaccgta aaggacgatg
     6721 actgaattat catacaagcc gctttctttc aaataattga agaattcttg tacggcggta
     6781 tctaaataat tggctgtcgc aaagtaaccg ttgatcgttt catcatttgt attggctaac
     6841 gggaatcctg cttcgtcatt ggtgaatttt gaataaggat aatggttaga tacagcaatg
     6901 aattttgaat aaaatggttg ttgcagacgt tccaagtatt gtacggattg ttggaaaaat
     6961 ggtttatcat gcaacccgta ttggaacgaa ttgtcatcat taacatcata ataagaagaa
     7021 tcaaagaaat aatcatatcc taaattcttg tatgtctcgt ttcgattcca gaatgtacca
     7081 gcgttcccat ggaataccgc actaatgtaa ccttgtgttt gacttaaaat atcaggtgca
     7141 gcttggaaag tattttttcc gcctaattgt gtgaacaagg agccttgatt caagccaaac
     7201 aatgaatttt ccatcagcgt ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa
     7261 aagttgtcaa aactgaacgt gctgttgctg tggtaaagac tattgatgaa aggtgtaact
     7321 tcatgctgga gaccattctc atctgtcaat ttataatcga tcaggaattg ctggaagctt
     7381 tctaaatgga tataaatcac attttttcct ctagcgagcc cgaacaaatc gtcatttggt
     7441 gctgcataat gttcttttac ataattttct acttctttca tatcattggg acttgcttct
     7501 gcccgcactt ggtttgtctg gtaagtttga acaccgtcat aaaccatgaa agcattcaag
     7561 ccaagatatt tgaccaaata atcacgagag aatgttcgag tcagcaattc tggacgatca
     7621 gcttccgcca taaaaagatt tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa
     7681 gccattcttg ctcggatcgg gcgagggtcc aacttgattt tttttgcaat cagcagaaca
     7741 gtcaagataa taaaatcgat ccaatagatc acatcatatg ggcgaaacag tcgaatggca
     7801 ctttctccca gaccactcgc tactttccca gcacctaaaa tcgtgttgat cgtaatgaag
     7861 tccgtgaatt ctcgataata agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa
     7921 atgaccatca tcgtgatata agcaactcta gggcgtctga tataaagagc aattccaagt
     7981 aataacaatg tagttgcaat agggttgata aacaagataa agtattgaat gccactttcg
     8041 attccgagat ggaaatcaac taaataggca aaaatcgttt tcaaccaaag taaaacgact
     8101 agtaaagaaa aaaatccgag cctcttattc aaaaaggcag gtgtttttat acatttcaag
     8161 ataatcttcc tttctgcact tcttaatcac agaagtgtcc tgaaaccatg ttacacagct
     8221 cacttattct catttatttt actagtatga caatatagtg tcaattgaaa ccagaagttt
     8281 atgctgtttc attaaggttt tctttaagct tcatgaaaaa atgagcagta agacaagtcc
     8341 tatgtttttt gttatacttg ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa
     8401 gtaaaaaaaa gagcaagcaa tcgattgaaa aaaggatatc cactgatcca aaaagaagac
     8461 cttgctcatc cccaaaaaga aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa
     8521 tttatcgcaa aaggatatct aggaaagcag aataagggaa tcggttgggt attgagccag
     8581 cagaacatcc catttgatca atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa
     8641 agaactttct ttttttcaga tgattctact accgcttttc gtatattcaa tggagaagga
     8701 gacgggatcg gcggtctgat tgttgattat tatgcaggtt atgctgtttt ttcttggtat
     8761 aacgaaacgt tatatgcaca tcgtgaatta ttgattcaag cttttcgtga aatctacccg
     8821 gaaatcatag gaggctatga gaaaattcga tttgaatcag taaatctgcc tgagagccag
     8881 ttcatttacg gaaaagaggc accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt
     8941 gccgtttatt taaatgaagg attaatgact gggatctttt tagaccaaaa agaagtacgg
     9001 ggatcattag cagaaggttt cgcttctggg aaaacagttt tgaacatgtt tagttatacc
     9061 ggcgcctttt ctgttgctgc cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta
     9121 gcaaaacgca gtttaaaaaa aacacaggaa cagtttgaag tgaatggttt ggatgtcgat
     9181 cagcaaaaaa tcattgttat ggacgtattc gagtatttca aatacgccaa aagaaaacaa
     9241 tttttatatg acatgattgt attggatcca ccaagttttg ccagaaataa gaaaaaagtc
     9301 tttcgcgtag caaaagatta tggggaactg gtcaaggata gtctggatat tttagcagaa
     9361 gacgggatac ttattgcgtc tacaaatgct gcgaatgtct cgatagaaca attccaaaca
     9421 atgattgaag aggaattcca tgccaaaaat gtttcttttg aacaacttgc tttatatcgt
     9481 ctgccaaaag atttccagac gacggatacc tttttagaag gcaattatct gaaagtgttt
     9541 gtttatcaaa ttaaaaataa ccattagaag gagcgatgca gaatggttcg ggtaaaggga
     9601 ttgaatatag gaagcggtag accaaagatt tgtgcgcctc ttatcggggc cgtccgagag
     9661 gaagtattac gtgaagcagt tcttgcacgt gaagcagggg ccgatttagt cgaatggcga
     9721 gtcgatcatt atcgtgaagt tttttctttt gatgaagtag cagaaacatt ggttcttatc
     9781 catgagatgc ttgatggact gccgttatta tttacgtttc gtaccttagc tgaaggcgga
     9841 gaacaagaca tatcgatccg tcagtatcgc gacctttatg aacatgtgat acacacagga
     9901 ttagtagata tggtcgatat cgagttgttc aaagtagaaa gtctaggaaa aagactgata
     9961 gaggaaatca agtctttaaa cattccttta attatcagta gtcatgattt taaagaaaca
    10021 ccagcagacc ctgtcttgct ttaccgattg aatgttatgg aacattttgg agccgatatc
    10081 ggaaaactgg cagtaactcc caataatgaa cgagatgttt tgcggttgat ggagttgact
    10141 agacgggcaa atgcttttgt ctccatgcct attattacga tgtccatggg aaatcttggt
    10201 aaaatttctc gtctggttgg cggaacgacg ggttccgtta tgacatttgg ttccttaagc
    10261 agcgattcta cttctgctcc aggacaatta ccggtcaaag cattgaagca aattattcaa
    10321 ttattaaatg aaccgttgta gaacaagttt gaggtgatat tttttggcaa agaaaaaaat
    10381 agtaaaaacg aatgcaatcc gtatggtcga acaaaaaaag attccctata cagagcacga
    10441 atatgagtgg gacgaaagcc acctaagtgc gtcatcggtc gctgaacagc ttccagaaag
    10501 ccaatctcgt atatttaaaa cactggttgc tgtgggcaat gtgacggggc ctcttgttgc
    10561 tgtgataccg ggagaagccg aattgaatct aaaaaaatta gcaaaagttt cagggaataa
    10621 aaaagtagaa atgctccatt taaaagattt ggaagcaacg accggataca tacgtggtgg
    10681 ctgctcgcct ataggtatga aaaaactatt tcctacttat ttagatcaaa ttgcagaaag
    10741 ctatgaacaa atcattgtct cggctggcag aagagggcta cagatggagt tagcacctca
    10801 agatatctgt gcattaacga gcggacaatt tgcagatata aaacaataaa tcatacgtat
    10861 aaaagaaaga aggaggccgg gatgttgttt gtccagtctc cttctttctt ttttatacat
    10921 tatgctcttt gcccggcatt cattcctgct acatgccctg tagcgaaagc cgctgtgata
    10981 ttaaagccgc cagtataacc attgacatcc agtaattctc ccgcaaaata cagaccatca
    11041 atgattttac tctccaatgt tttaggataa atttccttta gagaaacccc tccgccagtt
    11101 acaaatgatt tctcaatagg aaaagttttg ttgattgata gcttaaactc tttgcatagt
    11161 tgaacaaaat cttgaatttg tttttgagag gcttgttgtc ctgtcagatg atccatttca
    11221 agccgctcaa gaaagaaaac taacaatcgc tcaggcaata taccatgcca agcattgacc
    11281 aagttctttt tggttgcttt acttttttct gtcaatatat gaataagctc ttgtttggtt
    11341 tgtgtaggga aacaatccag agaaagtgtc actggttctc ccgttttctc taattcttta
    11401 ttgacaaaac ttgaacaacg cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt
    11461 aaatccatcg tatgttcagt caatacacga ccttttttat ttaaaacgcg taaagtgatg
    11521 tcttgtaaag aaagcccttg aagtgttttc tcctgaatat acggttcatc cgatatcaag
    11581 ggggactctg tcgcataaag aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac
    11641 ccatctccag tagaaccagt agatggataa gtccgtccac cagttgttaa gataatggca
    11701 ggagcatgga actcttcgaa ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc
    11761 aatttctcta ctcgagtaga gaatagaagc gtcacatcta gttctttcag acgattgagc
    11821 aatgcttcga taatcgtttt tgatttattt gttacaggaa acatccgtcc atgatcctct
    11881 tcttttaaat gtacgccttg tgattcaaag aagttcatga tatcgaaatt attccattgc
    11941 gcaaacgtac tatataaaaa ctttccattt ccaggaatat gtgcaatcaa atcatcaaca
    12001 ggacgattgt tcgtaacatt gcaccgacca ccacctgtca tcagtagttt ttttcctgct
    12061 tttttgtttt tttcgatcaa gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc
    12121 atcatcccgc ttgttccagc gccgactacg atcacatcat acagtttttc cattccctca
    12181 cctcaaggaa agtgtagcat atctgaagaa agaaaacaga aaaaaatgtt ttgagccaaa
    12241 agagaaaatg agctaaaatg agggaagata agcaaaggag cgtggaacga tgacaaacgc
    12301 aacagaatat gttcaagcaa ttcaagaaaa gctacatcaa aaggatcaag ggcaagaaga
    12361 atttttacaa gcaatcgatg aattcatgcc cacggttatg tcttttttgg acactcatcc
    12421 cgaatatatc gaaaagaata tcttagaact tttaacggaa ccagaaagag ttattcaatt
    12481 tcgagtgcct tggcaagatg atgaaggaaa ctggcgagta aatagagggt accgtatcca
    12541 atacaactca gctatcggcc cttataaagg tggattgcgc ttccatccta gtgtcaatct
    12601 aagtatcttg aaatttttgg cttttgaaca gattttcaag aatagtttaa ccggtctgcc
    12661 aatcggtgga ggaaaaggcg gaagtgactt cgatcctaaa ggaaaatctg ataatgagat
    12721 catgcgtttt tgtcaaagtt tcatgctgga attgtcaaaa catatcggcc catcgattga
    12781 tgttcctgcg ggagatatcg gtgtaggagc tagagaaatc ggctatttgt tcggcgaata
    12841 taaacgattg aaaaaatatg atactggtgt gttgacaggt aaacctttgg atttttgggg
    12901 aagtaagata cgtacggaag ctactggtta tggattagtg tattatgtca aacatctatt
    12961 gaacgaagaa aaagattcat ttaatcaaaa aacagttttt gtttccggaa gcgggaatgt
    13021 agccatttat gcaatcgaaa aagtgcaaga gctaggcggg aaagttgtta catgttcaga
    13081 ttctagcggg tacatctatg atccagaagg aatagatgtg aaactattga aagaagtcaa
    13141 agaaatcaat agacaacgtt tgacggaata cgcaaaccaa cgtccttctg ctatttatca
    13201 tgctggagaa tctgtttgga cattgaaaga aaaagcggat atcgctttgc catgtgctac
    13261 tcaaaatgaa atagacgaaa gcttggccga aatcttagta gaaaatggga tcaaaatcgt
    13321 agcagaaggc gctaatatgc catgtacagt aaaagctgtt tctgttcttc atgaagcagg
    13381 cgtgtggtat tgtccaggaa aagctgctaa tgctggagga gtagcagtat ctgctttaga
    13441 aatgagtcag aacgtccagc gattgacttg ggaaagagag caagtggatg agcagctgga
    13501 tcaaatcatg gaaacgattt accatacgtg tcgccaaaca gcagccgagt acggcgacga
    13561 aaaaaatctg cttcttggag caaatgttgc tggatttgaa aaagttgcca aagcaatggc
    13621 agcagaaggt ttagtttgaa aaatgaaact ttcttggaaa tattgtcagc attttgttta
    13681 tttgcttgaa aaataataaa aaaaaacgta aagtattacc tgtagagtaa acctataagg
    13741 aggagaacaa atggcacaca ttcattttga ctattcaaaa gtagcaccgt ttgtcaacga
    13801 acaagagtta gggtacatgc aaagccaagt aacagcagcc cacaacgaat tacgggaagg
    13861 tactggcgca ggaagcgact tccgcggctg gatcgattta ccaacgaact atgacaaaga
    13921 agaatttgca cgtatcaaag aagcagctaa aaaaatccaa tctgattctg aagtattagt
    13981 tgttatcggt atcggtggtt cttatttagg cgctcgtgca gcgatcgact tcttgaatca
    14041 cacattcttc aatttattag atagcaacac aagaaaagca ccacaaatct tctttgccgg
    14101 aaattcaatc agctcaacat atattgctga cttgatcgaa gtaatcggtg atcgcgactt
    14161 ttcagtaaac gttatttcaa aatctggaac aactactgaa ccagcgattg cgttccgtgt
    14221 attcaaagag cttcttgtta agaaatatgg acaagaagaa gcaaacaaac gtatctacgc
    14281 tacaacagat aaagcaaaag gtgcagttaa agtagaagca gatgcagaag gttgggaaac
    14341 tttcgtgatt cctgatgaca tcggcggacg tttctctgta ttaactgctg ttggtttgtt
    14401 accaatcgct gttagtggtg cagatatcga tgcattgatg caaggtgccg ctgatgcaag
    14461 caaagcatac tcaagcgaca aactagaaga aaacgaagct taccaatacg cagcaatgcg
    14521 caacattctt taccgtaaag gcaaagtaac tgagttgttg atcaactatg aaccaggaat
    14581 gcaatacttc tcagaatggt ggaaacaatt gtatggtgaa tctgaaggaa aagaccaaaa
    14641 aggcatctat ccttcaagcg caaacttctc aactgatttg cactctttag gacaattcat
    14701 ccaagaagga cgtcgcaata tttttgaaac agttgtgaag gttgaaaaac ctcgtaaatc
    14761 aatcacaatt cctgaacaag cagaagattt agacggtcta ggctacttgc aaggaaaaga
    14821 agtggacttc gtaaatacaa aagcatttga aggaacattg cttgctcata cagacggtga
    14881 cgtgcctaac ttgctagtca aaattccaga aatggatgct tatacattgg gttatacaat
    14941 gtacttcttt gaaattgctg taggtatttc aggttacttg aatggaatca acccattcga
    15001 ccaaccaggt gtagaagctt acaagaaaaa catgttcgca cttctaggca aaccaggctt
    15061 tgaagatctt gctaaagaat tgaacgaacg cctgtaagat aagggttttt cgctagattt
    15121 aaataatcag tgatagaaac atgttcgctt taaaatgaaa actggtaccc atttaggtac
    15181 tcaaacatac tgcatcgttt attcagaata aggggctgtg acaagacgtt tgtcacagtc
    15241 ccttgtttgt atagaaatcg attgattgcc ttgctcttta gtttgtttta ggactttaag
    15301 aacagtgagg ctgacttcga aggttaattc tgtttttttg ttttctagaa tcagtaagca
    15361 aaaaagcact cacttcgagt gcttttttgc gatatattct tagcttaaga taggcctaat
    15421 ttttctttta cagatgaggg taccttatcg ctggtaatta cttttttctc tatgatccgc
    15481 ttttcgcttg ctttcaactg caagatcgtt ccttcggggt aagcagaacc attggcagag
    15541 tcaccagaag taatgacatc gaaatctaac tgtctttttt caccgttttc agcataagca
    15601 tctacgttgt atttaaaacc tcttccaatt acttccccat tatctgcttt gatatcagtt
    15661 tctgcaggca ttggagcttt gactaccgca taataatctt tacctacata agtagactga
    15721 tagtagtccc aacctttcca tcctataaaa gcaatcaata tgataacgat caaccctaaa
    15781 atttttttca tgaaagatct ccttttttat ttttatta
//
LOCUS       DAIGOK010000064        15719 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.064, whole genome shotgun sequence.
ACCESSION   DAIGOK010000064 DAIGOK010000000
VERSION     DAIGOK010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.064"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            140..307
                     /locus_tag="NOA01_001617"
     CDS             140..307
                     /locus_tag="NOA01_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HCI0632810.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(369..2180)
                     /locus_tag="NOA01_001618"
     CDS             complement(369..2180)
                     /locus_tag="NOA01_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCI0632811.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(2177..2542)
                     /locus_tag="NOA01_001619"
     CDS             complement(2177..2542)
                     /locus_tag="NOA01_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HCI0632812.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(2705..3100)
                     /locus_tag="NOA01_001620"
     CDS             complement(2705..3100)
                     /locus_tag="NOA01_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCI0632813.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(3118..4080)
                     /locus_tag="NOA01_001621"
     CDS             complement(3118..4080)
                     /locus_tag="NOA01_001621"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HCI0632814.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(4080..4292)
                     /locus_tag="NOA01_001622"
     CDS             complement(4080..4292)
                     /locus_tag="NOA01_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HCI0632815.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(4313..5011)
                     /locus_tag="NOA01_001623"
     CDS             complement(4313..5011)
                     /locus_tag="NOA01_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCI0632816.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(5031..5573)
                     /locus_tag="NOA01_001624"
     CDS             complement(5031..5573)
                     /locus_tag="NOA01_001624"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCI0632817.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(5705..6709)
                     /locus_tag="NOA01_001625"
     CDS             complement(5705..6709)
                     /locus_tag="NOA01_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCI0632818.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILLSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(6706..7695)
                     /locus_tag="NOA01_001626"
     CDS             complement(6706..7695)
                     /locus_tag="NOA01_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCI0632819.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(7692..8498)
                     /locus_tag="NOA01_001627"
     CDS             complement(7692..8498)
                     /locus_tag="NOA01_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0632820.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            8664..9620
                     /locus_tag="NOA01_001628"
     CDS             8664..9620
                     /locus_tag="NOA01_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCI0632821.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(9697..10215)
                     /locus_tag="NOA01_001629"
     CDS             complement(9697..10215)
                     /locus_tag="NOA01_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCI0632822.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(10303..10452)
                     /gene="rpmG"
                     /locus_tag="NOA01_001630"
     CDS             complement(10303..10452)
                     /gene="rpmG"
                     /locus_tag="NOA01_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCI0632823.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            10680..11126
                     /locus_tag="NOA01_001631"
     CDS             10680..11126
                     /locus_tag="NOA01_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HCI0632824.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(11321..13216)
                     /gene="asnB"
                     /locus_tag="NOA01_001632"
     CDS             complement(11321..13216)
                     /gene="asnB"
                     /locus_tag="NOA01_001632"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HCI0632825.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(13541..14515)
                     /gene="bsh"
                     /locus_tag="NOA01_001633"
     CDS             complement(13541..14515)
                     /gene="bsh"
                     /locus_tag="NOA01_001633"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HCI0632826.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGERKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
     gene            complement(14849..14936)
                     /locus_tag="NOA01_001634"
     tRNA            complement(14849..14936)
                     /locus_tag="NOA01_001634"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14902..14904),aa:Ser,seq:cga)
     gene            complement(15081..15647)
                     /locus_tag="NOA01_001635"
     CDS             complement(15081..15647)
                     /locus_tag="NOA01_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCI0632827.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
BASE COUNT         4901 a         3282 c         2636 g         4900 t
ORIGIN      
        1 atatggtaaa tttataataa aacatttagt aaacattttc aaggattttt tgcaaaggtt
       61 tacaaacaaa cgtaaaacta gccttttttt ctcttttgtt ccataataga tataacaaat
      121 aataaatgag gtggattcaa tgaaaaaatt tgtatctgga atattagtag gaagtctggc
      181 aacagccgct gccgttgctg gtttggttgc ttctgtgaaa aaaacagtga tcgatccaat
      241 tgacgaaaaa gaagcaatga tcgaagaaaa ccgtaaaaaa gcaatgcgta aacgcgtttc
      301 tcgttaataa ttatttaaaa atacgcatga agactttcta aaaaacagat aagtcatcat
      361 gcgtattttt atttgtcagt caatataagt tgtgctattt tatctgcctg tctcagattc
      421 catggatctg tataaggtct agctactgct gcagttagcc aatccacacc ttcaacaggt
      481 attccatagc aataaaagtc agactggatt tcccctgaag catcaatcac gcgattcgta
      541 cctcgctcaa catctatagc ttctgaatga taacttcctg tattatctga aaatgaaaaa
      601 gggcgaacaa gtttgttttc cttcaaattt tttatgacag gattacttga acgagaaaag
      661 cttgtttgtg gcaatcgagc ttcaatcaaa tagcgactgc tgatttcttt atcttgtaga
      721 gaaataacat agcggttgtg cttttgctga atatcaaatg gcggttcgat caaatgaaaa
      781 atacccgcat ctatcagtgc tgctaattct ttcgtccgaa tgattggcgg accgatcgtt
      841 aaaaagctat ttagatgagt aaaccatccc catagtattt ctttgtattc cttagcagaa
      901 aaaacgtgcc attcaatagc ctgccggatt ggatcacgcc agtcttttaa tgcatcaaat
      961 gttgaagcaa atgctcctgt gcaattgcct ttttccgctt cttttatctg cccttcaata
     1021 aatattcggc tagcttctga aaagggctca ttctgatagg caagaggagt ttctaaatac
     1081 gaccaatccc atctataagg gattagtgct ggatgctttc cgagacactc ctctgaagag
     1141 agggagagaa aatcatgctc aaaatttttc tttggaatcg gcaagtgata ttcttcgatg
     1201 attttcttgt aataaaacaa ctcaacgtct tttttcagat agtcaaaaaa tgtttcgcca
     1261 gtcaaaaatc ccgactgatg ccattgctcc aggttttctt ttgttagtaa ccgtggccat
     1321 gccattgctc cgccttgctt ttgatttctt cctcgagggt aataagggag cccccttctt
     1381 gatccactgt atacaatcgg ttccttgcct gatggatgat agaccagttt tcccttttcg
     1441 ttctcgaagc gccctcctct tgatactgta aacaatccga cataatcaaa aaaacttaag
     1501 cctagacctc ttaaaaagac attttcttta ggcggtatct tcgaaagatc tacatctgca
     1561 ggattagaag gaagctgata cagtaaaaca tttttctttg cgtagtctac ccacactctt
     1621 tcttcttctg tctcatcatt ttcccagtga ccgcttgcta aaacaagctg atcgatagaa
     1681 tagctttgct gatctgtctg taaaagataa ccatcttttc ttttgcttac tttttttact
     1741 aaagaataaa caaactgaat agaagctgga aattcttctt gcaactttga gaaaaaccag
     1801 cgttgataga gtccgtataa acatcgagta gattgctgat ctttttccag acgattgatt
     1861 tcttctataa ttagttgctt atttttggtt accgtctgtt cgatataatc tgtagcaggt
     1921 cctttactcc attgaaacaa gttgggtccc tgtgttacca atcctccact ggacaatgtt
     1981 tcatctgtaa aaagcgtcac ttgttgagaa acagaattca ttaataactc cggtggctga
     2041 ttcaaccgcc aaactcgacc tccaggacca ttgggatcga aaagaaaaag ttcaagagaa
     2101 tgtctgtcct gattattatg gagaatcctt tctgctacac ttaatcctct aggtccggca
     2161 ccaatgatcc ctattttcat ttgcatgcct ccatttcgat aaattttttc aataaaacag
     2221 catggttctg atgttcattt tttgcaccat aaacaaaagt aatgactgga tgttctttca
     2281 taagttgact gagtttaaaa atagcttgct tcttttcttc ttcgaaaatc aattcttgct
     2341 ggtatttctt tgcaaatgaa gcaaatcgtt ctggtacatg gtcaaaagtt ttgcgtgctt
     2401 cattactagg tgcaatttct ttcaaccaca gatctaactg cgctttttct tttgaaattc
     2461 ctcgtggcca gaatctatcc acgagtattc tatagccatc ctctttttca gcatcaaggt
     2521 aaacacgttt taatttgatc atagttgttt ccttctttct accttttcgc ttagcttata
     2581 tatgtagata atagcacaaa ataaaaaaaa tgaacggcac aactatccat tgcgccgttc
     2641 atgtgatctt ggtcaaaatc taatgattga ttcggttttt aaagctgtca tacaatcatc
     2701 tgttttatac ttttttactt ttgatttttc cagaccatcc gtcatatcca cccttaagta
     2761 aataaatctc ttgataacct gctttacgta atttatttgc tgtacggatg ctcaaacttt
     2821 tcttttgatc gtaaatatag acaggctggt cttttcgaat agatcctaat gaatctttca
     2881 aaacagtgta agggatgttt cttgcaccta aaatatgccc agcatcaaac acatcttttt
     2941 cgcgaacatc gattacttgt gcttttctca ttccttctcg aaattcttct tctgtcaaag
     3001 tcgttgctga acgtttggcc attactcgta gatagacttc ccaaaggatg attgcaagca
     3061 caatcaatag caaaatgata ttaattaccc ataaaaccat gtaatgcctc cgttttttta
     3121 agcaaatttc aaggctaatg aggcagcacc aatgacacca gcttcattac caagctctgc
     3181 taatttgatt tttgttgatt ctcttacttg cgggaaagta aattctttaa aatatttctc
     3241 tacacggcta cgtaaaaatt ctcctgctgc cgatactcct ccacctaaca cgatacttga
     3301 cggatttaat gtattcccca agtttccgca agctaaacct aagtagaagc atacgcgatc
     3361 aacaaccatc aatgcaaacg gatcgtcttc ttgagccatt tcaaagatat ctttacttgt
     3421 tacttcttct ccgttgtcta gcattgcttt cagttttgaa tcccctgcgt attcttctgc
     3481 caagaaacga cctaggcgta ccacacctgt agcgctagat acagtttcta aacatccgcg
     3541 tttaccgcaa gtacattcga acccttcagg atccacagtg atatgtccga tttctcctcc
     3601 agctccgctc acgccgtgaa tcaaatttcc gccagcaacg atacctccgc caacacctgt
     3661 tcctagagta acgaatacaa cgtctggatc gttttctcca gcacctttcc atcgttcacc
     3721 taaagcagca acattcgcgt cattatcgat agcgaatttg attcctgttc ctgcctcgat
     3781 cagttttttc acaaattgaa cttctgtcca gtttaagtta tacgcaccaa taaccgttcc
     3841 agcttcgctg tccacgcttc ctggggtacc catgccgatt ccgataaagt cttcggcttt
     3901 catattatat aaattcaaac gatgattgat tgattcaatg atttcaggaa cgatatgctt
     3961 accatcatcc aaaatattgg tatcgatgct ccatttttgc tgaatctctc cttctggtgt
     4021 taaaattgca aattttgctg ttgttccacc taaatcaatc ccaataatct tcttgtccat
     4081 tacaaatttc ctttctttcg gctttcttca attcggtgct ctctttttaa tatgcgccac
     4141 gctgtcatgt atgtatcatg atcaatcaaa cggctatcat acagattacg caattcgata
     4201 gtcattaatt caatgtcata gatgcgtgca cccacataga taaagatgcc aaattttttg
     4261 aacagctgct gtacatcata taagctctcc atctttaagc acttccttca tattatacaa
     4321 gtaaaccata tttttttaaa cccagtaaaa tacatattac aaaaagaaat acaaaaacta
     4381 gtgtagaaag aatcctttgg tgtatcgaat agcttgtctg ttgtttcggt agacccaaaa
     4441 cattccctaa cactaatccg ccaattagcc caccgacatg tccccaaaca tctacagagg
     4501 tatccagaag accaaaaaca aatgtcaaca caataaataa aagaaaatgt ctgactagct
     4561 gctggatcgc tgggttatgt ttaaaatgaa accctaagat aaacaacgca ccgaacaaac
     4621 cgaatatcga agtacttgca ccaccagaca gaacattcgc ttcgttaaaa gcaaagcttg
     4681 ctgtatttcc cattatcccg ctgaacatat aaatcaagaa aaaacgccaa tggccataga
     4741 ttgcctcgat ttgctgcccc ataaaataca gtatcagtga gttcattacg aaatgcatca
     4801 gtccaaaatg gatgaagatt ggtgtcacta aacgccacca ttcattgaag tgaacaagat
     4861 aaggaccata cattccggca taataaggga tctcaagtcg cggaaataat tccatcatga
     4921 ggaagaccgc tgtctgtatc cctaaaaaag cataggtcca taacggtcgt cttaaccatg
     4981 cacgcaattt tatttgctgt tgataattca tgaaaacctc ccatttcatt ttagtcaata
     5041 aacaattgtt ggatcttctg atcaaagctc tccggttgcc attgttcatt caattgttca
     5101 cgaaaaacaa ggctacatgt ttttttatga aaattattta aataccggtc gtaataacca
     5161 ccgccaaaac cgattcgata accggctcta ttgtagataa tacctggtac aatgaggaga
     5221 tcgatcatct ccggcaaata gagcttctct gattgtggct caagaacacc aaaactcgaa
     5281 cgtacaagaa cggactgttc atccatttca taaaacgcca tctgacgttt cggcaaagtt
     5341 ttaggtacaa ctattttctt ttgttctttt tctgctcgtt ggataaccgg ctgtgtatcc
     5401 agttcaaatt cattcgataa agtcgtccct atcacttttg ctgacttcca gtactcgctg
     5461 gcgaatagct gtttatagat tcgctgttct ttttcctttt tcttgccact tttggccaga
     5521 tttttcagct tttcgattcc tttccggcgt aatcttactt tcaattggtc caaaaactca
     5581 cccttccttc tctcataaaa tggtatagga aaaaaagaaa aatagcaatc gaactttcaa
     5641 ataaatgcat aaatagaata aaaagaagac ctagaaatga ttggccttcc ttttttaatg
     5701 aatttcaact gttcagcgcg tgtttttgtc tgataaaaag tgcatacaga aaatatggcg
     5761 caccaaaaag ggcaatcaaa attcctgtag gtacttcccc acttagtaga atcactctag
     5821 caaccatatc tgcactaagc agaaaaatgg aaccgaacaa accgctcaga acaaaggtcc
     5881 agttattttc tttacgtacg actagcttcg cagcatgagg agctagtagt cctacaaaag
     5941 ataaactacc tgtcaccgct acactaccgc ttgccaaagc aacagctaac aacaaaagga
     6001 ttttttgact tttagccacg ttaaggccta atccttgcgc gcgttcttga ccaaatgata
     6061 gcaatacaat cctctgtgca taaagtcctc ctagcggaat taagataacc gcccatggaa
     6121 gcaatgccca aacataaggc cagctcgcac cccagattga tcccgctaac catgcattca
     6181 caaatccata attttcctta gataattgga tcgtgcatag caatgttaat gcagaaaggc
     6241 cggcattaac ggcaataccg gacaataaga ttcgattcat acccaaaaag ccatttcgat
     6301 cataggccgc aagatagaca cagcaagcag ctaaaacacc acctagaaag gctacaacta
     6361 gcaatagaaa cggcagcgaa tagtgcgaaa agaaacctaa ataaagcatc actaaaaatc
     6421 ctgctccggc attaatgccc aatatactag aatctgccaa ttcatttctt gttactcctt
     6481 gcaagatata accagaaacg ccaagacaag ctccagctaa taaactgatc agtaatcgag
     6541 gaactcgaaa ttctctaaca atcagcgcca aagaagatga ttctttccct atgaacgtgg
     6601 caaaaagctg atccaggtaa ataaaaggcg taccaatcat taaactacct atagacagga
     6661 tgacaagtac aaaaagcaag aaatagaacg tccaatatat ttttttcacc gttgttccct
     6721 ccttacttgt atcagtaaaa acggaacgcc aatcaatgca atgactaatc caaaaggtgt
     6781 ttcaaaaggg ggattgatca tcctcgctgt aaaatccgcc aacataacca atccaccgcc
     6841 aactaaaaaa gagacaggta gaacataacg atagtccgtt ccaaccattc cgcgaacaac
     6901 tggcggaacc aacagcccaa taaatgaaac aggtccgact aacgaaacgg caatggctgc
     6961 taacactgca actagacaga aaacaatcaa ccgcgttcgt ttaggatgac ttccaagagc
     7021 taatgcatgt gtttcactca aatgaatcaa agatattgct tttcctaatg aaaaagaaac
     7081 caaaacagca agtatatata aaggaaacaa tactgctaat tgtttccatg tgatattggc
     7141 agctccgcct acaaaccaaa aagcaagatc ctgctgcaga ttaaagaatt gggtaagtgc
     7201 ttggctgata gcagtaaaaa aagaactaat agctacgcca gctaaaatca gctgtaaagg
     7261 attcatgccc attttcgaaa actgcaaaaa accaaatacg agcaaagtta cagcaaaaga
     7321 acctgccaat gacattagaa acacagaaaa atcagaagct ttaggccaaa aagcaaaact
     7381 aagggccatt gccaacgaag cacccgcgtt gattcctagt aagcctgaat ctgctaaagg
     7441 atttcttgtc actccttgca tcaatgcacc gctcaatgca aaacaactcc ctacaagaaa
     7501 tgctcccgcc acacgaggca aacgaatatt tcgtatgatt tggtgacttt gttttgatct
     7561 atcaaaatca aacaacgcct gccaaaaatc tgcttttccc acagtcgctg cgccatttga
     7621 aagagcaaca aaaaaaataa tcaaaagaac aaaacagacc aaaagcagga gtctattttt
     7681 cttcatagtc ctcataattg atcctcctgc tcttttggca acaaatcata ggttagaaaa
     7741 actggtttat ttgttcttgg aagcgtgaca atcgttgcat caatctcaaa tatttttttc
     7801 atacactctt ttgtgatcac ttcttcaggt gtaccttctg tgatcaactt accgtttttc
     7861 attgctaata aataatctga aaaacgtgaa gcatgattaa tgtcatgaat cgacatcaag
     7921 atcgttgttt gtttcatttg attgatttca gtcaataact gtaggatctc taattggtgt
     7981 gccggatcta aaaatgttgt cggttcatcc aagatcaaga tatctgtttc ctgagcaagc
     8041 gccatagcga tccagacacg ctgggtttgt ccaccagaca gcgttgcgat atcccgcata
     8101 cgaaatccag ctagacctgt agcatccaac gcccactcaa ttgcttcatg atcttgcgtg
     8161 tccatgccac tgaacagttt ttgataaggg tatcgtccgt aagatactac ttcttctact
     8221 gtcatacctg tagggtgttc actagtttgt gctaataatg caattttttt tgcaagttct
     8281 tttgaactgt atgtagagat atcagagtta ttgacaatga tctttcctga tttcacaggt
     8341 agtatacggg taaaactttt caataaagta gattttccac ttccatttgg cccaatcaaa
     8401 ctagtgattt tcccttcagg aatcgtaaca gacatgtttt caatgatttt tttgttttcg
     8461 tagccggctg caatgtcagc tgctcttaat ctcgtcattt cacccatcct ttcgctgaaa
     8521 atcattctca attatgacgc tgtttcaaca tttcgtcaag cgctactttc atatctatag
     8581 ccaaatgaag cggttgacag cgttcgaaat atcaggaata ataaccaatg agaaagattc
     8641 tcaattatgt taggaggaaa gaaatgaaat taaaaacaaa agtattcaca ttatctagca
     8701 tattgctgct ggctggagta ctgagcgctt gctccccctc ttcagataaa gaaacagcta
     8761 caactgccaa ttcgacgact tcatccaata gtactcatac agtcacagat acacttggac
     8821 ataaagtaga gattccaaac gcgcccaaac ggatcatcgg cagttactta gaagactatc
     8881 tgattgcact aggggaaaaa cctatcgccc agtggactgt tggcagtggt tccattcaag
     8941 attatctgca agatgattta aaggatgtcc caacgatttc ttatgacctt ccttatgaga
     9001 aagtgttaag ctttgagcct gatttgctgt tgatttcttc ttccgctact gtcgaaggcg
     9061 ggaaatacga acaatatagc aaaatcgcgc caacttatgt tgtaaaaaac ggaacagatg
     9121 tcacatggga agaacaacta aaagatgtcg gcaaagcttt ggcaaaagaa aaagaggcag
     9181 aaaaaatcat ttccgattat caaaaatcca tcaagaaaac tcgagaagaa ctagcagata
     9241 aaatcgagaa caagacagct gcagtgttgt gggtgaccaa caactcagcc tttatggtca
     9301 gcaaaaatcg gtcaagtgga agaatcgtat acgacgatct ggaatttggt gttcctgatc
     9361 tagtggaaga ggtctccaaa aaagccactt ctgactggtc tgctgtttcc tctgaaaagc
     9421 tagctgaatt agatgctgac tacatcatcc ttgtgaacag tgacaaagat gctgccatgt
     9481 tcaatgaacc aagctggaag aatctaaaag cagtgacaga caatcatgta ctgcaattcg
     9541 gacctgaatc tagttggcta tataacggac ctattgccag tcaggaaatg gtcaatgata
     9601 tcaaagaaaa actaaattga ttttaactaa aaaaagcatc ttcacgatta gtcgtgagat
     9661 gctttttttc ttattaacaa actagacgca tagattttat tcttcttttg aagcaaccct
     9721 attttttact ctttcccatt tttgaaaacg atgggcatat agattttctt gatccgttat
     9781 cccctcatac tcctctataa gggcgaagtc tgaaaaatca atattcccaa tatacgtatc
     9841 gccttcaaac gtgctatcga tcaatgttcg ccagatccga ccagtgtaag gaagaagttc
     9901 ttgaaaaatc cgactaccac ccgaaacata gatatcttct gagatcgttt gactatacga
     9961 caaaagttca tcgatcgaat gcattacttc cgcattttct ttcgtgcgaa aatttggttg
    10021 cgttgtcaac acgatgatat ggcgataagg aagtgaaaga tcccccattc cttcataggt
    10081 ctttcttccc atcactaaaa ttcgatccat tgtgtgttcg cgaaagaaac gcatatcatt
    10141 tggcaatcgc catggtaaca aaccattttt gccgatcaga ccatttttat cttgtgccca
    10201 catggaaata aacatattat ccgctcctta tgattttaga tgttttgaga taaaaaaagt
    10261 ctcaattctt tgaccagcaa ggaattgaga actttaaaaa cattattttg tttcacggtg
    10321 caaagtaact ttgcgttcgc gtgggcaata tttttgtttt tccaaacgat caggattgtt
    10381 acgtttgttt ttgcttgtta gatagttacg ttctttacaa gaagtacact ctaaagtgat
    10441 gtttacgcgc atgtttttcc ctcccatttt tacaagaatc ctctaagatg actctcagac
    10501 ttgattatct taccatgatt ttttctagat tgctagtgtt tttcacgatt ttgttaagta
    10561 aaactacttg acgataaata caacggtttt acttctcaaa caaaatcttt ttctttttgt
    10621 ataaataccc ttacacttgt ttatatagat tatgtttttt aataaagggg gatactaata
    10681 tgccatggaa catgaaggac tttcctgcat caatgaaaaa cttagataaa ttgacaagaa
    10741 aaaaagcaat cgacatcgca aatgcattat tagatgaagg gtaccctgat aaccgtgcga
    10801 tcccaattgc cattgatcaa gcaaaaaaat ggtatgaaga tgctagtgaa tcagaaaaac
    10861 gaactttcga aaaagaaaaa aatccaagca agacggatga acacgatacg aatcctcgtg
    10921 ctggaaaact gcttgattca gatgtgatcg tagaatatga ggaagaacaa tggatcgtga
    10981 aatctaaagg ggcaaaaaag gcaagcaacc attttgatac aaaaaaagaa gcaatcgaaa
    11041 aaggaaagca agtagcacaa aacaaagaat caactttgtt catttataaa aaagatggga
    11101 caaaagaaaa agaaatcgat tattgaacat atgtaaaggg gttgtgacga aaatcgtgtc
    11161 acaaccccct tatgttcgaa acaatggtgt tcctctgcca actccgaacc ataagtcata
    11221 atatctataa aacgggaaga gctgtttttt gaaattgttt cttatcactc agtgttaaac
    11281 ggtaggagaa aacctctttt ttattgatat ttgattttca tcaaacagtc acatagtcga
    11341 ttgcttctgc ttgcggaatc gtttcatcta caaagtagac tttataccat gtcaagaaac
    11401 tgaagattgt ccagattttt ctttgctctt ctttacgtcc ttcgtgatgg tcatctagca
    11461 acttcatgat tttttcttga tcaaaaaatt cttttgcaaa atctgcttcg aataatgctt
    11521 tgacttgttc gtagccgtgt tcttctttca accaagcttt gattggcaca gggaatccta
    11581 atttcacacg attcgaccat tcttccggaa gatgtttgtt cgcagcttgt ctgaaaatat
    11641 cttttgtgtt gttttgattc aataaaaact tacttggtat ttgttgagcc aatttcatga
    11701 cttcgatatc caataaaggt actcggactt ccaaagaact tgccatagaa agtttatctg
    11761 cctttaacaa gatatctttt ggcatccatt gatgaagatc gacatattgc atcttattga
    11821 cttcatcttt gattccttct gttttctcgt aatgaacagt catgatttca tccacagatg
    11881 gtgctgtttg atattttggt gttaacactt ctgatgcttc tgattcttcg aacactcgag
    11941 catgaccgat aaaatattct tttgcaggag caagtgattc atataaatgg atccgtccat
    12001 ggaaattttt catttttccg attttacgtc ctaaattata gcgtgttcct tttggtaatt
    12061 ttttcaatcc ttgagcaatc acacggatga attttgaatt cgtatggaat ccatacgttt
    12121 ggtatcccgc aaacagttca tccgcccctt ctccagattg gactacccgc acactttgtg
    12181 aagcaagatt tgctaaaaag tacaaaggta cgcaagaagg atttgaatca ggttcatcta
    12241 agtgatactg gatcaaaggg aatgctttga acgacatgtc tccgtcgatc actgctgctg
    12301 tattatctaa gtccaatagt tcggttagtt ttctggcttc aatcgcttca ttataggttt
    12361 tatcatcaaa cccgattgag aaagaatgat ctggtttcaa tacagaggtt acataactgg
    12421 aatcaacccc gctggataag aaagaaccaa cttctacatc actgaccgta tgtgcttcaa
    12481 tagaagcttg aacggtttcg tcgatttttt cgatccattc ttgtcgggaa tgcgtttctt
    12541 ttgtttcaaa atctgcatcc caatattgct gaatgtctat ttttccatct ttatacgtat
    12601 aataatggcc ttcaggcaaa cgatagacat ctttgaagaa tgtttcgccg tttaatggag
    12661 aatattggaa agtcatataa ggtttcaacg cttctttgtt caattctttg ttaaaatcag
    12721 gatgaggcag aaaacttttg atttctgaac caaacataaa tgttccgttc atttctgcat
    12781 aataatatgg tttgatccca aagtggtctc ttgcaccgaa taactcattc ttttcattat
    12841 cccaaatagc aaaagcaaac attccgcgga tcttttgcaa taattctgtt ccccattctt
    12901 cgtaaccatg caaaaggact tctgtatctg catgtgtttg aaaaacgtgg ccagctgcaa
    12961 tcaattcttc tctcaaaggc tggtaattat agatttcgcc attaaaaata atgatctttg
    13021 tccgatcttc attgtaaata ggctgagtac cgccctctaa gtcaataata cttaatcttc
    13081 tgaatcctaa agcaacatgt ttatcgatat attctcctga actattaggt ccacggtgaa
    13141 cgattcgatc catcattgta ttaataattg tttttttgtt gtccttgtcg ttaacaaaac
    13201 ctacaatacc gcacatttaa ctttcacatc ctaatataaa attactgttt atttcattca
    13261 tatgcatctt atcatagaat aaatagcgga cgaatatttt ctctcttaca atttcgtcac
    13321 gtgaaaacaa aagatgaaag tttctgtttt tacagaaaat aaagtcccaa tatctaattt
    13381 accatgtttt cttttttgag gctaccttat tctcttcgtg ttaaaaaaat attagtggat
    13441 gctatttcca aaaaaaccag caaaaaaaga cacgacctaa agatcgtgtc taattatgac
    13501 tgtatcagaa aatatgatac caaacggatc tattcattaa ttaattgaca tagtttatct
    13561 gttgctcttt tatgactgga taactgacca attcatgacg atccaagtct tctttattca
    13621 tatttacagc agtaatttga ctgtcttcat acgtacgata ataataaatc cctttatcaa
    13681 cgttacagca agaagaataa atcgtatatt cgtattttct ttctccagta tcgcatactc
    13741 ctttttgttg ctcgactgag cctagtatat gaaaaaattg actgatactt tcagattctg
    13801 aatcacctga tgcagcattc attttagtaa atgtggcttt tacaaatcga gaaacagaag
    13861 agagatctcc aggtaagccc aatcctccca tcccacgact ataaacattc aagctgatct
    13921 ggtttgaaaa atgattttct ggtgttccat ttgatagcga gcgataattg ttcagattaa
    13981 ataattgata atcaaatggc ggattatttg tcaacacacc gacaggatta tcatataaat
    14041 gaagtccatc tttcatactt tcaattacta tcgatttttc tttatcagct aataaccagt
    14101 gtaaaggtga caagggtagt ttttcactaa aatcgatatt gactagattg atagtagcca
    14161 ataattcttt agcttcgtct atggttgagc attgccctag tatccatgga ataaactcaa
    14221 atggtgcaac gttatctttt ccttcttgta attctttata atcagcattg ccaggaaaat
    14281 tcagtcctgc catactcaac cctttttcat ttgtagcctc ataatagagt ggatagttgg
    14341 ataccccagc tgctattcct atcattgcat aatgtttatc taaatttttt acctttctaa
    14401 aatcgaaacg ataatttctt ggagtaatag tgaccacttc attataagac atctcataat
    14461 caaaattcct tccaaaataa tggtctttcg ttgcataagt gattgcggta cacatcgttt
    14521 cccgtcctaa aacaaatcgt ttttacactt ttatcttaca tctagtattt tttttatcaa
    14581 aaaaaggcca aagaagattg atttactagc atttataaaa aatagtactc agggggaatc
    14641 aaaaaatatt gtaggttaaa agtacaacaa taaggggctg tgacaaaagt cttgtcacag
    14701 ccccttattc cgaacaaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa
    14761 acgaaaaaat aggaaaagct atttttgttt ggccgttctt attacttgat gcaggacagc
    14821 ttcttcacaa cctctaatcg ttgatataag gaaggtacag gatttgaacc tgcgcgccgg
    14881 tattagccgg ttcgccggat ttcgagtccg gtgcattacc actctgccaa ccttccgtaa
    14941 gtactatttt taaacttttt tcacgaaatg tcaagcctat ttctaataga taaataacag
    15001 aaatcgcttt ttttagcaaa aaagtccatt gacagtttgc agccaacgga cttaccgatc
    15061 aaattcatac tgatagaaca ctatctgatt atttcaggat tatctaatgc taaaatcgag
    15121 tcaattgtcc gtttgatcag tgcaatgatt tgttcttctg ttggatgcat gatgtttgat
    15181 gacatcaatg acgctaagcc agtcactgct acccatgtac cagtatgcag agcatcgaca
    15241 tattcatccg ataattgatt gtatctttca tcttctttaa ctatttcacg gaaataattg
    15301 tatgaaaaat catgcatttt ctttccgtcc ccatgttcat caatgtacaa tgccatgtaa
    15361 agactatgat gcttttttgc aaagtagata tagttcaaac cgagatcaat gattttatca
    15421 ccagtacgtt caatagggaa aacatcttct tttagttgtt caaagataga atccagcaac
    15481 gtatttttta aatcttccat gtttttgaat tccagataaa tcggctgtgt cgaaatcccc
    15541 atctttttag caatatttct cgcagtaaat cctttaaacc cttcctttgc aacaacatca
    15601 taagcagctt ttagaatttg atccttggta tagacttttc ttctcattaa aactccccca
    15661 cttctttact gcagtatatg ttgttttatt gtaccctatt tttagcattt tttgcaaac
//
LOCUS       DAIGOK010000065        15299 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.065, whole genome shotgun sequence.
ACCESSION   DAIGOK010000065 DAIGOK010000000
VERSION     DAIGOK010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.065"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(22..252)
                     /locus_tag="NOA01_001636"
     CDS             complement(22..252)
                     /locus_tag="NOA01_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632828.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(688..1254)
                     /locus_tag="NOA01_001637"
     CDS             complement(688..1254)
                     /locus_tag="NOA01_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCI0632829.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1254..2210)
                     /locus_tag="NOA01_001638"
     CDS             complement(1254..2210)
                     /locus_tag="NOA01_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCI0632830.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2210..2866)
                     /locus_tag="NOA01_001639"
     CDS             complement(2210..2866)
                     /locus_tag="NOA01_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCI0632831.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3236..5254
                     /locus_tag="NOA01_001640"
     CDS             3236..5254
                     /locus_tag="NOA01_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCI0632832.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5324..5530
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5681..8095
                     /locus_tag="NOA01_001641"
     CDS             5681..8095
                     /locus_tag="NOA01_001641"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCI0632833.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8443..9996
                     /locus_tag="NOA01_001642"
     CDS             8443..9996
                     /locus_tag="NOA01_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HCI0632834.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10377..12161)
                     /locus_tag="NOA01_001643"
     CDS             complement(10377..12161)
                     /locus_tag="NOA01_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0632835.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12176..13897)
                     /locus_tag="NOA01_001644"
     CDS             complement(12176..13897)
                     /locus_tag="NOA01_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0632836.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLTGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13997..14758
                     /locus_tag="NOA01_001645"
     CDS             13997..14758
                     /locus_tag="NOA01_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCI0632837.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         4978 a         2799 c         3000 g         4522 t
ORIGIN      
        1 tctcattaaa aagcataacc attactccga tctgttttta aaattagttt ctacctctaa
       61 tatatatcga gtttttattt tgccttcaga gaatactgtt tctttttttg cagttacagg
      121 ttgtttattt tcggaaaaag ctaatatagc taagattgaa acatccatct ggaatttatc
      181 ttttttactg ctttgatcat aaaagtctgc atattcatca ctgatatttt ttctaataaa
      241 ttcttccatc ataaaaatca cctcaaaacg attatacgaa cttacgttct tgttgtaaag
      301 cgatatttga ggggcaaaaa aggggcataa tttgactgat attaagttag ctattgatat
      361 atgaatgaca tacaaaaata taaaatcttt taaaaatgct tatatatcaa tgtttttgcc
      421 attcttttat atatctgtta tatgtcaaaa aaatgtgttc aatttcaatt tgtatttgaa
      481 taaagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa tcaaaaacag
      541 cttctcatgt ttttgatttg gttggactta ttaccgcaga tataagcttt tgagcaccgt
      601 gaataaagag gttatgaaat caacgctcta acctgagtat tagagaaaaa aacgcaaaac
      661 atatcattta tgttttgcgt tttttattta tctttttttc tcaatacaat ataaaatagg
      721 cggctggttc ttttggttga taaaccgata attcagcaca ttatatgttt cctgtggcag
      781 tttggaacaa aaagaatcaa ccatatccag ttctttttct ccaccttcat gaccatagta
      841 gacgacaagg atcaatcgtc cgcgaggcac aagtcgtttc aatatttctt ccatagctgt
      901 gcgtgtagtc tctggcatag tgatgattgc tttatcactt tttgggagat accctagatt
      961 gaaaatccct gctttgagat tttgttgttc agctatcact tctcctaacg tttcgtggcc
     1021 ttgtagaaac aatgagacac gttcttctaa ctgacgctgt tctagccttt cacgagtagc
     1081 atcgattgct tgttgctgga tatcaaaact atagacatga ccagtttttc cgatgtgttc
     1141 tgctagaaaa accgtatcgt ggccattgcc catcgttgca tctacgacgt gatcccctgg
     1201 ttgcaagatt tcttgcagta atgtatggct gaaatgcaaa gcagtttgca acatcagacg
     1261 ctcacctttt tccgtacatc atattttcct tggaagctat ctctgcgaag aagttcttca
     1321 tcaattgcat tcaagacttc ccattttttc aagctccaca ttggtccaat caaggaatcc
     1381 cacggcgcat cccctgtcaa acgatggatg atgatctctt ttggtatcat ttccagctgg
     1441 tcgcaaatga cattgacata gtcaggacgg ctcattaatt gaaggcgacc ttcatgatag
     1501 tcccgcaaca ttctcgtttt gcgcatcaga tgcatcaaat ggagcttgat tccttggata
     1561 tctgaatcta aaatggtgcg tctgacattt tctttcatca tttctaaact ttcgcctggt
     1621 aatccgttga tcaaatgcgt acaaacacgg atattatgtt tgcgtaattt agccacgcca
     1681 tctaaataag tttgataatc atgtgctcgg ttgatcagtt ttgaagtttc ttcaaaagtt
     1741 gtttgcaatc caagttctac ccataaatac atccgctcgt tcagttcagc aaggtaatca
     1801 actacttcat ctggcagaca atccggtcgt gtaccgactg ataatcctac gacacctggt
     1861 aaattgacta cttgctcaaa tcgttctttg attatttcaa ctggcgcatg ggtgtttgta
     1921 aaattttgga aataaacgat gtattgttgg acatgaggcc atttttgatg catcatgtcg
     1981 atttctttct ggaattggat tggaagaggg tcttcaggtg caacgatcat gtctcccgaa
     2041 ccagatacac tgcaaaaagt acaaccgcca tgggcaacag tcccatctcg attcggacag
     2101 tcaaatcctc catcgatcgg caccttgaaa attttcccgc caaactcatt tcgtaaagcg
     2161 taattccaag aatgatatct tttatttcct tctgaataag gaaaagtcat taaaagtcct
     2221 tctttctgta agagttatct atttgttttc ttttttctct atacactgga taagtcaggc
     2281 acagccaaga aagactgagt aagtagccgc cgatgatatc agtaggaaaa tgaacgccca
     2341 gataaatgcg gcttatcccg acacttacta taataaacat catcagcaat tgaagcaatg
     2401 tgcgtaaggt tcttttttga acaaaagctg gaagcagaaa tagtaaagaa ccgtaaagaa
     2461 tcatactgcc tgctgcatgt ccactaggga aactgtaact tgtttcaact actaagtgcg
     2521 ccaatgaagg acgctctcgc atgaaaaata gtttgatcac cgggttgatg attccagcaa
     2581 ttacaataaa tccagaaacg aaccaaaatg tttctgccct ttttttctta taccacatcc
     2641 ataaagccgc acaagcaaat aaaatgatga cggtaagagg attaccgaat ttagtcataa
     2701 agagttggta gctgttcaga tcaggataag aagctctgac aatgcctgta aaccactgat
     2761 cgaaaccaga aagccacgac agatggaact ttacgatcca gcccaaaatg aaaaacagga
     2821 caagaaagaa gctaccagcc acctgaaatg atgttttata tttcatacaa ttcctccttt
     2881 gaagcaatga cttattatag catttttggt ccagcgaaaa aaacggtaaa gagataaaac
     2941 caatgtatga ttcctctttg aataaccaat attcttataa aaaaaataac tgtaatcgga
     3001 aagacatttt ttggtaatgc aaatgaaatg ttagggaaat aaaatcagtg tttttcgaaa
     3061 tccttttaaa caagaacgta gacgtaggaa gaacttgaaa atgaacagaa gttgttcatt
     3121 acggtaaatt acaatatgat tacaaaaaac aacaaagctc ttaaataact tttaataaat
     3181 attaagagaa gttataatta ttaataatag ataaattgaa gttggaggat agaagatgga
     3241 aaacattgca agaaaagaac ggagacgttt agaacagacc aaacgttttc gaaaagcaaa
     3301 acgaggggca gcaatcgtgg gaactgcgat ggtaggctgt tctgtcgctg ccccattagt
     3361 acagccggtt cctgttcaag cggatgaaac acctgttcag ttcggcgcaa gaatcaatac
     3421 agcggcattt atcacagaaa ttgctactta tgctcaacca atcgctcaag ccaacgatct
     3481 atatgcatca gtgatgatcg cacaagcagt tgtagaaagc ggttggggca gtagtgcgtt
     3541 atctcaagca ccttattata atctttttgg gataaaaggc agctatcaag gacaaaccgt
     3601 ctacatggat acgcttgaat ttttaaataa tcaatgggta aataaaaagg agcctttccg
     3661 acaatatcct tcttttgctg aatctttcaa tgacaatgcc tatgtactta gaaatacttc
     3721 atttggcaac ggttattact atgccggtac ttggaagagc aacacgaaat cctatacaga
     3781 tgcgacagct tgtttgacag ggcgttatgc gactgatcct ggttacgcag gcaaattaaa
     3841 taatatcatc gcaacttacg gattgaccca atatgataca ccagctagcg gaaacgcagg
     3901 cggtggagcg actacaggtg gcaataatac tggaagtgca ggtagtactg gcggttcaag
     3961 caattctggt aatacaaact caggttcagc aacttcgacc acttatactg tccaatctgg
     4021 tgattctgtt tggggaatct caaataaatt cggtatcaca atggatcaat tgatccaatg
     4081 gaacaacatc caaaacaatt tcatttatcc aggacaaaaa ctgacaatca aaggtagcca
     4141 ggaaaatggc tcttcgacga ataattccgg taacaatacc aactcttcag gaaatgctgg
     4201 tacatcaaac ggaggacaaa caactggtgc gaaatacact gttcaatctg gtgattctgt
     4261 ttggaagatc tcaaatgatc acggaattac gatggctcaa ttgattgaat ggaacaatat
     4321 caagaacaat ttcgtttatc caggacaaca gctaatcgta tcaaatggcg gttcttctaa
     4381 tgctggagca aatactggct caaatactgg gaatacaagt ggctctagtt cgtccagctc
     4441 aagttcttct aatacagctt caggaacgaa atatacagtt aaagccggag actctgtttg
     4501 gagcgtctca aataaatacg gaattacgat ggctcagcta atccaatgga acaatatcca
     4561 aaataacttt atctacccag gacaacaact gactgtttca aacagtggat caacaaacag
     4621 cacgtcaggt aatacaggtt cgtcaaccaa tacaaataac agcagttcag ttaatactgg
     4681 cagcagttcg tctcaaggca cttataccgt gaaagcaggt gaatctgttt ggagcgtggc
     4741 aaataaaaac ggcatcacga tggatcagtt aatcgaatgg aataatatca aaaacaactt
     4801 catttatcca ggacagcaat tgatcgttaa aggtggaact tcggcaaata caaatacagg
     4861 ttcaactact tctgcagcga aaccaaatac accgaatacg tctgctacga caagtacatc
     4921 atccactggt aatacgatgt ataccgtgaa agcgggcgag tcagtttgga gcgtagcgaa
     4981 caaacatcat atcacgatgg atcagttaat cgaatggaat aatatcaaaa ataatttcat
     5041 ctatccagga cagcaagtga ttgtgaagaa aggttcatct caatcaacaa accaacaatc
     5101 agttactggt tcgaaaacct atactgtgaa atctggagaa tctgtttgga gtgttgctga
     5161 cagtcatgga attacaatgg ctcaactaat cgaatggaac aatattaaga acaactttat
     5221 ttatccaggt caaacgttga ttgtagctaa ataaaatagc tgttcgaaga aataacgatt
     5281 gaattccaca aaaatatttg ttactataag gctacttaaa tatgacaaca cttgaaaagg
     5341 aagtagtaaa aagactgaag tttcagagag cgcatggctg gtgagaatgt gcacggatgc
     5401 ttttgaactc gcctttgagt tgcccttgct gaatacccga gtaagcaagc ggcggtgacg
     5461 gccgttacaa cgttcgaggc cttttcacta aggtaaaatt aggtggtacc gcgtagatta
     5521 cgtcctataa aaagagcaat ctttttatag gattttttta tttcttttgt gaagtttgag
     5581 aaaacaaagc aaaaggaaat aaaaataaac agattctgcc ggttttttcg ttatatttat
     5641 aatagaaaca agtgttttca atagaacagg aggacagcaa atgagttaca atcataaaga
     5701 aattgagaaa aaatggcaaa aatactgggc aaaaacgaat actttcaaca cccatgatga
     5761 tcctgaaaag ccaaaatttt acgcactgga tatgtttccc tatccatccg gtcaagggct
     5821 acatgtaggg catcctgaag gttatacggc aacggatatc ctttcacgtg tgaaaagaag
     5881 tcaaggatac aacgtgctgc atccaatggg gtgggatgca ttcggtctgc ctgctgaaca
     5941 atatgcgcta gatacaggaa acgatccagc tgagtttacc caaaaaaata tcgaaacatt
     6001 ccgacgccaa atcaattctt taggatttag ttatgactgg aatcgcgaag tgaatacaac
     6061 tgatccggag tattataaat ggacacaatg gatctttacg aagctttatg aaaaaggtct
     6121 agcttatgaa gcagaagtag cggtaaactg ggtacctgaa ttaggaacag ttatttcaaa
     6181 tgaagaagtg atcgatggga aaagtgaacg tggtggctat gatgtagtca gaaaaccaat
     6241 gcgccaatgg atgctgaaaa tcactgctta tgctgatcgc ttattggatg atctagaatt
     6301 agttgattgg ccagaaagca ttaaagaaat gcaaagaaat tggatcggtc gttcagtagg
     6361 agcaaatatt gaatttaaag tggctggtac ggataaatcc tataccgtat ttacgacacg
     6421 accagatact ctattcggtg caacttactc ggttcttgca cctgaactag atcttgttag
     6481 agaaattacg acccctgaac aaaaagctgc tgtagaagct tatatcgaag agacagctaa
     6541 aaaatcagat ctaaagagaa cggatcttgc aaaagaaaaa acaggagtat ttactggtgc
     6601 ttatgcaatc aatccagtta atggcaagga aatcccaatc tggatcgccg attatgtatt
     6661 atcttcatac ggcacaggag ccatcatggc tgttccagca catgatgaac gtgactttga
     6721 attcgctcaa actttcggac tagaaattct tccagttatc gaaggtggag atgttgaaaa
     6781 agctgcttac acagaagatg gtacgcacat caactctgaa tttctagacg gcatgaacaa
     6841 acaagaagca atcgataaaa tgaatgaatg gctagaagaa aatggcgtag ggaaaaaaga
     6901 agtcagctat cgcttacgtg actggctatt ctcccgtcaa cgttactggg gtgaaccaat
     6961 cccaatcatc cattgggagg acggaacagt cacagctgtc ccagaagaag agctaccatt
     7021 acggttgcct aagacaaaaa acatcaagcc aagcggtact ggagaatctc cattagcaaa
     7081 tatcgaggag tgggtcaatg tcgttgatcc tgtaacaggt aaaaaaggcc gtcgcgaaac
     7141 gaatacaatg ccacaatggg caggaagctc atggtattac ctgcgttata tcgaccctca
     7201 taacaaaaaa gaactagcag attatgaaaa gttgaagcgt tggctaccag ttgacatcta
     7261 cattggtgga gcagaacatg cggtacttca tttactatat gcacgattct ggcataaatt
     7321 cttatacgat atcggtgttg ttcctacaaa agaaccattc caaaaactat acaatcaagg
     7381 aatgatctta ggcgaaaaca acgaaaaaat gtcaaaatct cgtggtaatg tagtcaatcc
     7441 tgatgatgtg gtcgaaactt acggtgcaga tacactgcgc atgtacgaaa tgttcatggg
     7501 gccattagat gcttcgattg cttggagcga aaatggctta gaaggaagcc gtaaattctt
     7561 ggatcgtgta tggcgtttga tcgttgatga gaacaataaa atgcgtgacc gtatcactac
     7621 attgaatgat ggcaagcttg ataaagtgta tcatcaaaca gtgaaaaaag tgacagaaga
     7681 ctatgaaaat cttcatttca atacagcaat ctcccaattg atggtcttta tcaatgaagc
     7741 ctataaagtg gacgctctgc cttatgaata tatcgaagga tttgttcaac tactcgcacc
     7801 gattgctcct cacatgggtg aagaactatg ggcaatcctt ggaaatgatg gtggtatttc
     7861 ttacgcacca tggccaacat acgatgaagc agcacttgtt gaagacgaag tagaagttgt
     7921 tttccaagta aacggtaaag ttcgtgcgaa aagcaatgtt ccccgagact taggaaaaga
     7981 tgagctagaa aaagcagcat tagccaatga aatagtgcaa gaatatattg aaggcaaaac
     8041 agtcagaaaa gttattgcgg taccaaacaa gttagtcaat atcgttgcta attaattggc
     8101 tctttgtcaa taaggactga tgagttgtgc aaaatcaaat ctgggccaga atgaacttca
     8161 ttctgtctca gattttttgt tatttcaaat tgattaattg attgatcaaa aaggttttga
     8221 ttttcatgtt ctcaaacacg ttcaatgtct ttttcttctg aatggtcttg taagagatct
     8281 ctgtaatcat caaatttttg tctattaatc ttagtatttt ttgatgtata aactacctga
     8341 aattgagagt acataccaaa ttcataaatt ttttattagt gggactagtg attgtatgga
     8401 ttttgacgca taatgaatga agtatgagaa aaggagagac aaatggatca agaactattt
     8461 attatcgaag tattatcagc taaacaaaaa attatcgaga aagcgaaaaa aagagcgaaa
     8521 agaataatgt tcaagctttt aagcttagta tttttctctg gtttgctttt tttcggagct
     8581 gtatcgttcc tatatcgcca tatgagtgca gtagtccgtt cagctgaagg gatgcaaaaa
     8641 ttaaataatt tgtatcactt ttcgtgggga gttggcggaa taacaattgt tgtgcttatt
     8701 gtttttcttc tacttatttg gctccgttac catcaattgt cgcgtcattt cgatcaatgg
     8761 cttagtcaaa gagcacaaca gtttttacat aataaaccaa cttctcaaga tgatgactat
     8821 ctttattttg agaagacttc gacgagtcaa gcttttcgga tacaaaaaag tgattgccag
     8881 ttattgctga gatcacttga tgatttacct atctacttag gcgaaagcaa atcaaaattc
     8941 ggaatgaaca cattccatat ttttttacta gaaaacgaac ccagatggga aaaaagccag
     9001 tcttcgcctg gtggatacct gacaactaag atgagtacat tagtcactgg ttttttcctc
     9061 attcttttag ctggaacaat ctattatggg agatacactc agccaaaaat gtcttctcat
     9121 ataaatgctg cttctcacat gacaatgaac tcaactaacg atacgcatat acaaaagaca
     9181 aatgaagggt tgttagctga tcctaagcaa aaggtaaatt tgacaacaca aaagataaat
     9241 gatctcaaac ttgatacaga gacgcactct ctctatatga caacagatag tggtgctaat
     9301 tgggtatttg tgccgattga ttcgcaatgg ttgcgttttg gcgaatacac cctgacaacg
     9361 gggctgattc ctgaaggtta ttggatggac aacacatatg atatttccgc cgatttctca
     9421 tggtttatct attcttctgg ggatgatgta cgtctgctga cttcaagaga taatggtaaa
     9481 acatggcaaa cgtctattat cacccaatca gttcagccga tacgttatcg aaaagcggta
     9541 ttttatggca acgatggtct atttatctgt acatcagcca gtgggatgtc tgcagaaact
     9601 ttgtacattt acactactat ggatggcgga atcacttgga taaaaagtgg aagtacaatg
     9661 atcaatcagc cagtacaaaa tgtcagcttc gttacaactt ctttaggttt tgtttcgaca
     9721 cgtgaaagtt tgttttatac aaacaacgga ggacgctcat tcaaagaatc agttctgtcg
     9781 attccggaaa actatcagac aggggggata gatctttttc aatcaccaaa tgaagttact
     9841 caaataagcg cgaatacgtt agagacaaaa ttttatttgt tgaaaaaagg accaatcgat
     9901 gcagggaaaa tgtttgcctg cctcttccgt tcaactgata acggggaaac atggcaattc
     9961 gttcagcaac tatcgcaagt agaacaagga gattaaagaa gagagattgt gagcctgacg
    10021 ttcagcaccg agtcacaaga aacaaattca aaaaacagct tctcatattt gttgaatagt
    10081 gtaactcgtg attcggagtt gacaagagca acaagaagcg attttgaagt atagcttctc
    10141 gtattttaca agatcgtgtc ttattgccga taggttgtct cttgaacacc gtttattcgt
    10201 aaaaagggat tgtgacaagt cttttgtcac tacttaccga acgaaactga aggagctatc
    10261 caaaaagtat cattagtatt attctttgtg tttctagtgg caccactagt acatttaaaa
    10321 aagctatcga tcatggacta atcatacatg atcgatagct ttttagataa tgtttgttac
    10381 gctatctgaa attgactatt ataaagactg gcataaagcg ttggtgattg gagtagttca
    10441 gaatgtgttc ctttttcgac gatatcgcca ttcttcatta caaggatcat gtctgcattt
    10501 tcaatcgttg acaaacggtg tgcaatcaca aaacttgtcc tatcttttgt cacagcttcc
    10561 attgcttgct gaatcaatgc ttctgttcgt gtatctacgc tagaagttgc ttcatctaaa
    10621 ataacgacag gaggattcgc tagaatgact cgagcaattg tcaataattg ttgctgtcct
    10681 tgagataacg tgccgttttc gctagaaatg atcgtatcat atccgtctgg cagtgttcga
    10741 ataaagtgat cacattgtgc gatttcagcc gcttcgataa tttgttctct tgtggcatcc
    10801 attttcccgt aggcgatatt atcagctacc gttccttcaa acagccaggt attttgtaaa
    10861 accatcccaa aagaacgacg tagttctcca cgtgaaagct tggtaatatc aagaccgtcg
    10921 aattcgatct ttcctttatt cagttcataa aaacgcatca gcagattgac taaagtcgtt
    10981 tttcctgcac cagttggacc aacgatcgct accgtttgtt ttggctttac tgaaaaatcg
    11041 acttgattca tcaatatttt atctgatgtg taaccaaact gtacattttt gaaagctatt
    11101 tttcctttcg gttgttctaa aagtcttgca tctggaaggt ctggaatttc atctgtttca
    11161 tctaatatct caaatatcct atcgattgct gccattgcgg attggatcga gttgatcaca
    11221 taagaagcgg tagaaattgg ttcagaaatc tgattgatgt actgaagata agcttgtaag
    11281 aagccgatcg tcacaccacc tgatagaaca agcatagcac cgaataccgc acttatgatg
    11341 aaggcgagtt gattaatgaa acgaatggcc ggatagatag cacagttcat aaattgagca
    11401 cgtttaaaag cgttgtattg ctttttattt acttcgtgta tagcagcttc tgcatgagct
    11461 tgctgattga atgttttgac cactagatta ccagctaaga tttcttccat tcgattattt
    11521 aaaacaccca gttcatactg attctgttca gaaatctgtt tgtttttgtt cgcaatctta
    11581 gttgtaacaa ttgaactgcc aacgatcagc aataagacga gcaaagtaag cttgctttcg
    11641 atataaaaca gcatcagacc agcagcagct attgttacga cagaagtaaa aaattgattg
    11701 atacctgtca ataacacttg agaaagttga ttgagaccgg tagttgtccg actaatgatg
    11761 tcacctactt gatgattgtc aaaaaacgac atcggtaatg ttttgaattt ttttgtgatt
    11821 tcttttcgca ccctcaatgt gatcttttcg cttaacgctg ccattgtccg ttcctgaata
    11881 aatgaagtga tgctgcttaa gactgaacag ccgatcagca ataagacagg gaacaacaat
    11941 gcttgtttga tatcactgaa agaaagctgt ccaatacctg tttttcttat ttctgataag
    12001 agatcatcga tcccgattcc catgatcaag ggcatggcta cgactaatag gttcccgaat
    12061 aaactgcata gaaacaatcc gtaaaaatat ttatgttcag gtttgatcat tttccaaaaa
    12121 cgtatcaaag cacgcgtgtt caacgttttt ttcttttcca tcgtatccct tctttctata
    12181 ttccttgtga ttttgcaaac tcctgataag tttggcaatt agacagtagt tcttcatgag
    12241 taccttgtcc aacgattttt ccttcatcta aaacaatgat gttatctgct tgttggattg
    12301 tactgaggcg ttgagcaaca atcacgagag ttttctccga catgtgttta tgaagggctg
    12361 ctcttaatgc tgcatctgtt ttatagtcta aagcggagaa actatcatcg aatacataga
    12421 tctctgctgg tttgatcaac gctcgagcaa tacatatccg ctgtttctgg ccccctgaat
    12481 aattagaacc gccttgagca acaaagctgt ccaatccgtc aggaagggtg tccactagtt
    12541 cttctaattg agcgatcttg atcgctttag acaactcttc tgttgaggca tattcatttc
    12601 ccatctgcaa gttgcttaaa attgtcccgc tgaataagaa agccttctga gggacataac
    12661 tgatgtggga gcggatctgt tcttgagtca tttgagtaag aggaatacca ccaagggtta
    12721 cttttcctat cgtagggtca ctaagtctca agagaagttt cgccaaacta ctttttccag
    12781 atccggtccc gccaacgatc gctgttgttt ttcctttagg tattgtgaaa tcaatatctt
    12841 ctagaactgg atcagagatt tcagtatatt taaatgtgac atgttcaaaa gcagctgcat
    12901 ttgtctgatc aaaggttggt tcagctactg ttttgatcgt ctcttcctca atttcgatct
    12961 ctgcttgcag tatttcttct atccgtcgta atgaggcaaa cgatttaggg ataatgacca
    13021 ataccatagc tgcgatcatc aagtaagcaa gtgtcagcat agagtattcg actacagcag
    13081 tcacgacacc gatttgtaat gctccttctg ctacaagatt tccgccaaac cacaaaatag
    13141 aagcataaac gattcccgtg agcaaaaaag caattggagt gacccaagca aatattcggt
    13201 tcactttgat catttgatta gcatagtcag caaagctctt attcgttcgc ttttcttcat
    13261 atgattgatt atcaaaggca cggatcatat taatcccagt aaagaattct ctcaatgtga
    13321 tggtgatccg atccattttt ggctggatac tcaaagataa aggtgcccct cgtttcatca
    13381 gtcgataaac aatccataca taaaaaacaa cagagatcaa tgtaacccat gttagtaata
    13441 gagaataacg aaacatcatg agaatggtga aaacgcaaat aaccggtgct ggtaagatca
    13501 tttggcaaaa taaaacaatc atttgctgga cattatcgac atcattcgtc attcttgtca
    13561 aaagagatcc agaactaaaa cgactgatat ccttcattga cagctgttgg aatttacgga
    13621 aatatttctc ccgcgtttgg aatccaaacc tagcggaagc caatgctgaa aagtaactag
    13681 aggcaactgc agcgattgtt cctagaaacg ctacaagcag catttcaact ccaatttttt
    13741 ttataactga ggcttcgcca cttgctatac ctgtgtcgat cagctttgct actaattttg
    13801 gtacacctaa tgtaccggcg atttctaaac agatcatcac aaaggtaagg agagcgagtc
    13861 ctttgttttt ctgtaagaat gtttttaaga tagacatatt attcctcctt ttttcctgat
    13921 acttttcgat tctatacctt caagtaactt gaaggtcaat agaaaaaaga ataaactgaa
    13981 aaagaggtga gaaaaaatga aaaaaatgta tacgattggc caagtatcgg aactatttga
    14041 tttgccaaaa tcaacgatcc gctattggga tgaacaagga ttaatccgtt caagtagaca
    14101 agaagagaat gattatcgat tgtttgatat cgatgatatt tttatgcttt atgatatcgt
    14161 tttttataga aaattagata tacctatcaa acagatgaag aatttatatg gaaaaacatt
    14221 gacggagtta tatgagacac ttgatgaaac agaaagaaga atccataaag aactggtggt
    14281 gatgaaacaa aaacaaaaag agataaggga gcaaaaaaaa cagttgaaat tgatgatcga
    14341 tacaaacgag gaagaatttc ctgtagaaga gatccctttt gactgtatga tctcaacgga
    14401 gtttgaagat attgtggaaa tcaaaaaatt tctacctaac tattcaagct ttgggatgat
    14461 gagtatgcca gcttccagtt cacagacgat gtatggattt tttatcgacc ctagtgaagt
    14521 acatttattt actcaagacg tgatttggga gaaaaaagat acggctgttt atcgtcgatt
    14581 cttattgaaa tccgagatga accatgcgga acgtaataat attttagaaa tcagagaaag
    14641 aatgtatgaa aaaggatgga aaacaggaga agtgatcggc cagtatctat tgacgaatac
    14701 ggatgaaaac aatatcagaa ctgaatatta tcatgcatgg atagaaatga aaaaatagga
    14761 aatatgtgtt taagataact cgaaaatgat ggttgtttta gttataaaga aaaattttct
    14821 ggttcgatta cgggtttaat gtgggggatt attagtcgtg gatagataga attttagttt
    14881 tttagaggaa atatgtcttt aaaaatggaa tggttgcaat tcttagcgat atatacgatg
    14941 taattgtcct cttaaaaaag tatggaaaaa taattgtatt gggtgttatg tataattcat
    15001 ggtgaactga gactaatgag aagaaaaatt catattgtta tgagtgagat tgtatcagat
    15061 gagaatgata aattaaatag gtgcataaaa gcattacact taaaatacat tgttgcttac
    15121 attattacaa tatgttaata aggcaaaaat aaatagatgg attgaataaa catatttttt
    15181 aatcaaatag tccctactgt gactttcctc agctatcggt gatactaccg atagcatttt
    15241 tttgcttata aaataattat aagtatttag agatttttct tctaacattc ttcaaacaa
//
LOCUS       DAIGOK010000066        14621 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.066, whole genome shotgun sequence.
ACCESSION   DAIGOK010000066 DAIGOK010000000
VERSION     DAIGOK010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.066"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..340
                     /locus_tag="NOA01_001646"
     CDS             <1..340
                     /locus_tag="NOA01_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding domain-containing protein"
                     /protein_id="HCI0632838.1"
                     /translation="IKNNSLSSSVSSFKKMFKSRDGITLTTAHKVKGNEYDTVIAINL
                     LNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSEVGYFTSKGDLLYPTQL
                     LDKIEFDYNI"
     gene            540..854
                     /locus_tag="NOA01_001647"
     CDS             540..854
                     /locus_tag="NOA01_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCI0632839.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            875..1252
                     /locus_tag="NOA01_001648"
     CDS             875..1252
                     /locus_tag="NOA01_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HCI0632840.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1262..2035
                     /locus_tag="NOA01_001649"
     CDS             1262..2035
                     /locus_tag="NOA01_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632841.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2057..3460
                     /locus_tag="NOA01_001650"
     CDS             2057..3460
                     /locus_tag="NOA01_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HCI0632842.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3642..4826
                     /locus_tag="NOA01_001651"
     CDS             3642..4826
                     /locus_tag="NOA01_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCI0632843.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            4823..5113
                     /locus_tag="NOA01_001652"
     CDS             4823..5113
                     /locus_tag="NOA01_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632844.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5110..5331
                     /locus_tag="NOA01_001653"
     CDS             5110..5331
                     /locus_tag="NOA01_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632845.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5373..6152
                     /locus_tag="NOA01_001654"
     CDS             5373..6152
                     /locus_tag="NOA01_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HCI0632846.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6219..6713
                     /locus_tag="NOA01_001655"
     CDS             6219..6713
                     /locus_tag="NOA01_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HCI0632847.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6769..7407
                     /locus_tag="NOA01_001656"
     CDS             6769..7407
                     /locus_tag="NOA01_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632848.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7479..7874
                     /locus_tag="NOA01_001657"
     CDS             7479..7874
                     /locus_tag="NOA01_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCI0632849.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            7858..10311
                     /locus_tag="NOA01_001658"
     CDS             7858..10311
                     /locus_tag="NOA01_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCI0632850.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10308..12335
                     /locus_tag="NOA01_001659"
     CDS             10308..12335
                     /locus_tag="NOA01_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632851.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12332..13354
                     /locus_tag="NOA01_001660"
     CDS             12332..13354
                     /locus_tag="NOA01_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HCI0632852.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13371..14300
                     /locus_tag="NOA01_001661"
     CDS             13371..14300
                     /locus_tag="NOA01_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCI0632853.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14545..>14621
                     /locus_tag="NOA01_001662"
     CDS             14545..>14621
                     /locus_tag="NOA01_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="regulates the transcription of tet(M) by
                     attenuation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HCI0632854.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDK"
BASE COUNT         4960 a         2453 c         2899 g         4309 t
ORIGIN      
        1 gataaaaaat aattcattat ctagtagtgt atctagtttt aagaagatgt ttaaatctcg
       61 agatggtatc acacttacta cagcacataa agttaaagga aatgagtatg atactgttat
      121 cgctattaat cttctaaacg ggaaaattcc tcattggaat gaaattttta atgtttctga
      181 taaaggaatt agttcagcga gaaaactact atacgtaatt tgttcaagag caaaaaataa
      241 tttatttctt ttctcagagg tgggttattt tacgagtaaa ggagacttgc tttatcccac
      301 tcaattatta gataaaatag aatttgatta taatatttaa aatacctagg tagtcgttgt
      361 tttaaatata gtaggaatcg gacgctcagt acggagcgtc tattttttta cccaattttg
      421 aaaggaagtg aacttatgaa aataaaaaat caagaatcaa aaggtcgctc tccccccttt
      481 aaaaccatca cacaaataag attcattcaa acataaaaac agaaaggata ggtaaaaata
      541 tggaacttaa atatgtcatt cccaatatgg aaaaaacatt tggacaatta gaatatgctg
      601 gtgaagggaa cattgaacaa cgtcgaataa atggcagacc aaccattctc tctcgtagtt
      661 acaatttata ctcaacgatt caacgagcag atgatattgt tgtcattctt ccctccgaag
      721 ctggcgagaa acattttgaa tccgacgaaa aagtcaaact catcaaccca catattaccg
      781 cagaaggtta taaaattggc aatcgaggat ttaccaatta cattatgcac gcagacgata
      841 tggtgaaagc ataagaaagg aaaggtgaaa aaatatgaga ttagcagaag gtattgtgat
      901 tgaaaaagac cagacatttg gcacattgaa attttccgcc ttgcgtcgtg aggttcgtat
      961 ccagaatgaa gatggtacgg tttccgaaga aattaaagaa cgtacctatg acttaaaatc
     1021 aaaaggacaa ggacgcatga tacaagtcag tatccccgct tctgttcctt tgaaagaatt
     1081 tgactacaac gcagaagtgg aattaatcaa tcctgtggca gataccgtag cgacggctac
     1141 ttttcgaggg gcagacgttg attggtacgt gaaagcagac gatcttgtat taaaaggaca
     1201 gacaaaacct catcagaaat ataaaaaaga agcgtccgaa agtgaaaaat aggtgttgct
     1261 tatgacaaac acaccattga ttttgaaaga aattccgaag gatgaagcca tttcttttat
     1321 tcggcaatac cattattcaa agatactccc tagattgtgt aaatactttc tagggatttt
     1381 ttcagaagaa aaattattag gcgttgtgga attaggctgg ggtacgcaac ccctacaaac
     1441 catacgcaaa ctctttcccg acagttccct tcaaaccaca gattatttag aaattggaaa
     1501 gatgtgtttt cttccagaaa tgaatcaaac caattatttt ggcagtcaag ctctttcagc
     1561 tctaattaag tggttgaaag aacatacgga ctgtcatttt ttatatacac tggcagatgg
     1621 aattgaggga aaatgtggct atgtctatca agcgtccaac ttcttttact gcgggtattt
     1681 caagacaagt gtctatcgtg ataagcagtc atgggaaaag atacaccctc gaagtgctcg
     1741 ccttttatta gaggaaaatg ctcgctttga acaggtagag aaaaagcatt ggctttccca
     1801 ggcattttgc gaatacaaag ggattgagaa aatcaatgga cgcatgtttc gctatctcta
     1861 tccgttgaca aaagaagcga aaaaactgtt aggacacact ctctatcgac gtcattatta
     1921 tccgaaagaa aaaaatttac gctttgaaaa gcgaatcgct tatcagaaat atgaagccat
     1981 ttctcaacca acttttgata aacaggctcg tatctacaat acacaattat tttgataggt
     2041 ggaaaggagg atttttatgc cattgcttca tgacagagga gagcgaatca aagccaacga
     2101 taagcacttg gtctatcatt ttgtactaat ttcattgctg gtagtttttc tagtcgtcct
     2161 actccctttt cattggagag cgttacaatc ggtgaactgg caagcagttc agttagatac
     2221 atttttctat caacttcata cgccatactt ttggagcagt attctcactg ctctttttgt
     2281 atgcggactt atcagctggc tttattatca ctatcgtata gattctataa aacagttaga
     2341 gcaccgtcaa aagttagctc gtatgatttt agaaaatcat tggtatgaag ccgaagaaac
     2401 tcaatcagag ggattcttta aagatttacc cagcaagaaa accaaacaac gaatccgcca
     2461 ttttcctaaa atatattatc gaatgaaaga tggactaatc catattcatg tagaaatcac
     2521 cttagggaaa tatcaagatc agcttttgca cctagaaaac aaactagaaa caggattgta
     2581 ttgtgaatta gtggaaaaaa cgttgaaaga ttcttatgta gagtatgtcc tactttatga
     2641 tactattggc aatcgaattg gcattgaaga cgtggtagct caagatggac gtgtcaaatt
     2701 gatggaatcg gtctattggg agttcgattc tctccctcac atgttaattg ctgggggcac
     2761 tggtagtgga aaatcctatt ttattctcac attgattgaa tctctcctgc atacaaatgc
     2821 aaagctttat attcttgacc ctaaaaatgc tgatttggca gacttagggg cggtcatgaa
     2881 taatgtctat taccgtaaag aagatatgtt ggcatgtatc aatcaatttt acgaagatat
     2941 gattgatcgc agtgaaacca tgaaacaaca ccctaactat aaaacaggag aaaattatgc
     3001 ttatcttggt ttgtctgcca attttcttat ttttgatgaa tatgtggcaa tgatggatat
     3061 gctaggtcgt gaaagtacca ccgttattca taaactcaaa caaattgtaa tgttaggacg
     3121 acaagcaggc ttctttttaa ttcttgcctg tcaacgtcca gacgcaaaat attttagtga
     3181 tgggattcgg gacaacttta acttccgtgt ggctttgggt cgtatgtctg aaatgggctt
     3241 tggtatgatg ttcggaagcg atacacaaaa acaatttttc ttaaaaccca tcaagggaag
     3301 aggttacgtg gatacaggaa aaagtgtgat tagtgagttt tatacacccc ttgtaccaaa
     3361 aaggtacgat ttcttaggag aaataggaaa agtcattcaa aaaaagcaat ctgagcctgt
     3421 accgcacgaa gtgaaaggta caggttcaga cttgctttga ttggtgtggc gttagccacg
     3481 ccaatccttt agccccctct atctaacagg ggggcacaaa tacaactgaa aacaggcaat
     3541 tctcatcaac caatgttgat ggtacaaagg attgcggaaa atatgaaagg tgtaaactca
     3601 agggtcatag tttacatctt ttttagattg gaggtttgcg aatggaaaac gaaaaaattt
     3661 ggattaagga attgaaagaa aagcgactgg cttatggcgt ttctcaaaat aaactggcgg
     3721 ttgcttctca tattaccaga ccttacctat cggatattga aacaggcaag gcagtcccta
     3781 ctcaacaggt gaaagaagat ttactgaacg ctttggaacg cttcaatccc gataacccat
     3841 tggagatgtt attcgactat gtacggattc gattccctac caatgatgtg gctcaaatta
     3901 tcggtgaggt tttacgtttg aatatggatt atatgctaca cgaagacttt ggctattatt
     3961 cttatcccga acactatcgt ttcggtgata ttgttgtcct agtctctcat gatgtaagta
     4021 aaggtgtttt attagaactg aaaggaaaag gctgtcggca atttgaaaac tttctattgg
     4081 ctcaacatcg gagctggtat gatttctttc aagattgcat ggaacataaa ggtattttca
     4141 aacgcttgga tttagctatc aacgataaaa cagggatttt gaatattcca gatttggcaa
     4201 agaaatgtaa acaggaagaa tgcatttccg tctttcgaag ctttaaaaat tatcgcagtg
     4261 gtgaattggt tcaccgtgat gaaaaaccat acatgggaaa cacactttat attggctcat
     4321 taaaaagtga agtctatttc tgtatatatg agaaagatta tgagcaatac gtgaaaaatg
     4381 atgttccctt agaagacgca gaagtaaaga atcggtttga aattcggtta aaaaatgacc
     4441 gagccaccca agccatgaaa gatttattgg cacatcaaca ggcggaaaaa acagcctttg
     4501 aaattatcaa tcgctatatc cgctttgcag ataaagacga cactaaacgt cggagtgatt
     4561 ggaaaactaa tgaacgttgg gaatggttta tcggtaaaaa tcgtggagca ttacgcctaa
     4621 ccacacagcc agagccttat tcttttgaac gaacattgaa ctggttacat catcaagtcg
     4681 cccctacatt aaaaatcgca agcattttag atgttctcaa cggtacaacc attatctcca
     4741 ccatgattca ggaagccaaa ctgacagaaa aacatgagaa attaattgaa caacaacatc
     4801 tagccatgga ggatttgatt acatgagcct tttaacaaaa gaattaaaga agttagggtt
     4861 tcagtgtggc attgagtttc aagcgtatat acaaaataca gggaaatata cgtctctcat
     4921 tattgaggga aaacggcaag ctggtgatac catctatacc tatgattttt acaaggtgag
     4981 attttataat aattatacca atcgagtaac cgtctatgga gagcatttaa ccccttttca
     5041 attattaagg cgtgtaaaaa gctatatcta ttatcgagaa aaatatctta aggaaaggag
     5101 gacaatcaca tgaattttgg gcagaatttg tacaactggt tcttggataa tgcacagtca
     5161 ttggtattgt tagcgattgt tgtcattgga ctgtttttag gttttaaacg agaattttca
     5221 aaactcattg gcttcttgat tattgctcta atcgccgtcg gacttgtgtt caatgcagct
     5281 ggcgtgaaag atgtgttatt aaacttattt aaccgaatca ttggtgctta acagatattt
     5341 ttcatgataa aataggaagt atatattttg ttatgaaagg atttatgaat atgtttacaa
     5401 atgatcaatt taaatctctt ttaattaaaa cattagaaag aaagagctct tcaaattatt
     5461 atgaaggagg taacgagatt gtttctaaaa tgaaaatatc agaggtaact ttagatgaga
     5521 ctgacgattt tacttattat aaaggatata aaaagggctg gaatacgctt tgcacatatc
     5581 ttaaaataag agttcctttt gatgatttag acttttttga aagtcataaa gatttgatta
     5641 cccagacagc aagctccata tatgataaac aaggtgataa tgtattagtg gatactattt
     5701 tagtaccact tccagagaat tacgaagtaa ttaactttag tcagttgaaa attagtgatg
     5761 tggtttcaca agctattgaa gacgctgagt cttttatgag taatggtgaa tatcaacgtg
     5821 cctttgacag agttcacact gcttttcatg gatatttaat tgaaatacta aaaaaatatg
     5881 aaattacagt tcctagagac gaaaatctat ctaaattata tagtaggata caacaattaa
     5941 ttgaaaaaga aattcaacct actgaacttg ctgatatagt gaagacaacg ataaggtcat
     6001 caaatggaat gattagctct cttaatgaag caagaaatag gcattcactt gcacacccta
     6061 atacaaatat tattggaaag agagaagcaa aactaataat aggtatttca agtacagtta
     6121 cagattatat tagcggatac cttgataagt agcgtcatta actgattgct acttttttta
     6181 tgactatttt tataaaaagg atgtgataag aattggaaat gaaagtctat gtggcaaatt
     6241 taggacggta taatgaaggt gaattagtcg gagcatggtt tactccacca attgatgagg
     6301 aagaaatggc agaaagaatc ggtttaaatg aggactacga agaatatgca atacatgatt
     6361 ttgagcttcc ttttgatgtg gacgaataca cgccgattag tgaaatcaac cgtctatgtg
     6421 aagccattca agaaatcgaa ggtacaccta tttataatga actcaaagaa attcaaggca
     6481 tgtggtttag tagcttggaa gaattactgg aaaacaaaga ggatattcat tgttatagtg
     6541 attgtgattc gatggaagat gtcgcccgtt attacgtgga agaaacagga cagcttggtg
     6601 aagtcccttc caatttacaa aattatattg actaccaatc tttgggacgt gatatggaaa
     6661 tagagggtaa ttacctagtt acctctcatg gggtatttga atattgtcag taagtttaac
     6721 aatccgtgtt ataattaaat taataaatag cacggagagg tataaattat ggtaaatgta
     6781 tttgaaaatc ttaaactatt aaaactagac atgaaagata aaggctgggt gatagattcc
     6841 ttttactttc gttacaaaca gcaaaattat attgtacttg ttaagctatt tgaaaaagag
     6901 gaaaaagttc cagaatatgc tctgttaaaa ctagaatttt taaaggaaaa cgatttttca
     6961 gatatgctag ctgtctatgc taattcggta aaattatata cagacacgaa aacaataaga
     7021 gaatattttg gtatcgaata cagtagtaac ttgggagatg ttttatttca atttagccaa
     7081 actcttgctc gatttattcc gacagaagtt agtgagaaga aaaatgaaga ccaaaaagaa
     7141 gccatgtgtt tctcattaag tcaaagtgat tcagaagatc ccagaaaaaa atattgtttt
     7201 tcagttagaa gaaatccttt aaagggaaat ggtgagctgg gaaaacgaag tccttttaat
     7261 gacaataaaa cgagattgta tagacccagc ttatatgaac gattagggtc agataccaat
     7321 ttaagtttca gatattcaat gaatccagat gatgaagaaa ctgatgaagg tattattgca
     7381 aaatggacaa aaaataaaat tgaatagtta aagcattgaa agagcaatca cctattttga
     7441 ttgttctttt tctattttta cggaaaggac gtgaagacat gaaaaaaata agaagttaca
     7501 ccagtatttg gtcagtagaa aaagtgattt atgcgattaa tgatttacaa ctccctttcc
     7561 caattacctt tacgcaaatg acatggttta tcgtttcttt gtttgtcgtc atactctttg
     7621 gcagtttacc accactttct ctgattgacg gagcactact caaatactta gggattccca
     7681 ttgttctcac tgggttcatg tgccaaaaga cctttgacgg aaagaaaccc tatggatttc
     7741 taaagtctat cattacctat gctctacgcc ctaaagtaac ctacgctggc aaacaaatac
     7801 gattaaaaaa agaaacaatg aatgaatcga ttacgattgt aaggagtgaa caactagatg
     7861 gcatatccga ttaaatatat ggaagataat ctcgtattta atcatgatgg cgaagtattc
     7921 gcttattatg aattaattcc ctataattac agttttttaa gttccgatga aaaaattcaa
     7981 gttcatgata atttctgtca attgattgcc caaaatcgtg atggcaagat tcatgctctg
     8041 caactagcaa ccgaatcaag tattcgagcc acacaggaac aatcgaaaaa agaaatcact
     8101 ggacgattaa aagagatagc ttacgaaaaa atagatgaac agaccgaagc gttaatttct
     8161 atgattggtg acaatcaagt agactatcgc ttttttattg gttttaaact ctacctcaat
     8221 gaacaggaag tatccgtaaa aaatgttggg aaagaaattc aaaaagcatt tgcggaattc
     8281 tttcatggtg tgaatcacaa attaatgggc gattttgttt ctatgcctat ggcggaaatc
     8341 gaacgctttt ctaagatgga acagttgtta gaaagtaaat tatccagacg ctttcagatt
     8401 cgtaaattgg agaaagatga ttttggttat attattgaac atctttacgg gcaaacaggt
     8461 gtcccttttg atgactacga ctaccattta ccgaaacgat acgaagataa aaaagtgttg
     8521 gtaaagaaat acgatattct caaacccact cgctgtttaa tcgaagaaaa tcaacgctat
     8581 ttacggattg aacaggaaga taccactact tatgtggcgt attttacaat taatagtatt
     8641 gttggtgaat tggatttccc taatagtgaa attttttatt accagcaaca gcagtttact
     8701 ttccccgttg ataccagtat gaatgttgaa attgtcgcca ataaaaaagc cttatctacc
     8761 gttcgcaata aaaagaaaga actgaaagat ttagatagcc acgcatggga aagcgacaac
     8821 gaaaccagta ctaatgtggt ggacgcttta gaatctgtcg atgaattaga aagtaattta
     8881 gaccaaacca aagaatctat gtacaaacta agctatgtgg tacgggtatc agcaaagaat
     8941 ttggacgagc tgaaacaacg ttgtgatgaa gtaaaagatt tttatgatga tctgtctatc
     9001 aaattagtac gaccttttgg cgatatgcta ggcttgcata atgaatttat tccctcaagc
     9061 aagcgatata tcaatgacta tattcaatat gtcacgtcgg attttttggc tggtcttggt
     9121 ttcggtgcta cacaaatgat tggtgaaaat tctggtattt acgtcggtta caatctcgat
     9181 acaggcagaa atatttatct ccaacctagc cttgcaagtc aaggaattaa aggctctgta
     9241 acaaatgcat tagcgtccgc ttttattggt tcattaggtg gcggaaaatc attctctaac
     9301 aatatgctgg tctattattc agtactcttt ggcggacagg caaccattgt tgacccaaaa
     9361 gctgaacgtg gtgaatggaa agaaacctta cctgatattg cccatgaaat caatattgtt
     9421 aatctcacga gtgagaacga caataaaggg ttacttgacc cttatgtgat tttaaaactc
     9481 ccgaaagatt cagaaagcct tgccattgat attctcacgt tcttaacagg tatttccagc
     9541 cgagatagcg agaagtttcc tgtcctgcgt aaagctattc ggagtgtaac tcaaagcaaa
     9601 caacgagggt tgttattggt gattgatgaa ttgcgaaaag aagatacgcc catcagcaat
     9661 agtattgctg accatatcga aagctttgtc gattatgatt ttgcccatct gttattcagt
     9721 gatggcagtg tcacccaatc cattagcttg gaaaaacaac tcaatattat tcaagtggca
     9781 gatttggtct tacccgattc caatagctct tttgaagaat acactaccat ggaattatta
     9841 agtgtggcaa tgctgattgt cattagtacc tttgctttag actttatcca ctctgaccgt
     9901 tccattttta aaattgttga ccttgatgag gcttggagtt ttttacaagt ggcacaagga
     9961 aaagctctat ctatgcgatt ggttcgagca ggacgtgcca tgaatgcagg ggtctatttc
    10021 gttactcaaa acgcggacga tttactagat gaaaagatga agaataatat tggcttgaaa
    10081 tttgcctttc gttccactga cctagtggaa atcaaaaaga ccttagaatt ttttggtgtg
    10141 gacaaggaag atgaaaacaa tcagaaacgc ttacgtgaat tagaaaatgg acaatgttta
    10201 gtttcagaca tctatggtcg tgtaggggtt atgcagtttc acccgatttt tgaagagttg
    10261 cttcatgcct ttgataccag accgcctgtg cgaaatgagg tggaagaatg acacaaaaac
    10321 aaaagaaatt tcttcactat agttggataa cgctcctttg tgtgggcgtt attttacttt
    10381 tattaggcac gctgggcaat gcagtacaag cgacaggctt agttgatgaa accattgata
    10441 caagcaatga atactccaag tatggtttga atcaatatca gcttgattat tatgtggaca
    10501 atagctgggg ctggcttcca tggaattgga gtgatggcat aggacaatcg gtgatgtatg
    10561 gattgtatgc cattactaat tttatttgga caatttctct ttatctatcg aacgccacag
    10621 gttatttggt gaaagaagcc tatgccttag atttcattag tcaaacatcc caagcaattg
    10681 gcgaaaacat gcagacgatt gcaggcatta caccgaatgg cttttccagt tcagggtttt
    10741 atgtcggttt cctccttctt tttattctca ttgttggaat ctatgttgcc tatacaggat
    10801 tggttaagcg agaaacgacg aaagtgatac gagctgtttt aaactttgtc gtggtgttta
    10861 ttctatcggc ttcctttatt gcgtatgcac ccgattatat tgcgaaaatc aatgattttt
    10921 ccaaagatgt cagtaccgcc agtctgaata ttgggacaaa aattgttctt cctaacacgg
    10981 atagtcaagg gaaagacagt gtggacatga tacgtgacag cttattctct gttcaagtaa
    11041 agcaaccatg gctattgctt caatatggct ccactgatgt agaagcgtta ggggaagaac
    11101 gtgtggaaag tttattgtct accagtccag atacaaacaa tggtgaagat agagaaaatg
    11161 tagtgattga agaaattgaa gatcatgaca atacctattt aaccctaccg aagacgattt
    11221 ccagattagg aacagtcttc tttttgtttg tattcaatat tggtatctcg atttttgttt
    11281 tccttctaac aggaatcatg attttttcgc aagtgctatt tattattttc gctatgtttt
    11341 tgccaatcag tttcctatta agtatgttgc cgagttttga tggcatgggc aggcatgcca
    11401 ttaccaagtt atttaatgtg attatgacaa gggctggtat taccttgatt attaccgttg
    11461 cctttagtat ttccactatg ctttattcgc tgtcagcaag ttctcccttt ttcatgatta
    11521 tgtttttaca aattgtgacc tttgcaggga tttattttaa actgggcgat ttaatgagcc
    11581 tattcacttt acaaagcaac gactctcaaa acatgggcag acaagtgttt cgcagacctc
    11641 gtcaaatgat gaatcgacaa accagaagat ttcaacgcag tatgagtaag atttttcgtg
    11701 gaaaagcagg gtcaacgaag caaaagacaa tgacaacaaa gcagaatcaa accaataaag
    11761 ctaaccatac tcgtcaaaat gaacggacta caccaaagaa agaaacagca ctttccaatg
    11821 tgaaaaagca acaaggcggt atcgggcaac gattaggtga aaagacagcc catgtattgg
    11881 acacgaaaaa tcgtctagtc aataaaagaa agcaggcaaa acaacagata caggcgacac
    11941 caacccatgt caaatatgcc cttcatcaag gaaaagagaa tgtcactcat aatgtaacag
    12001 actttaaaca atctatggca gataccaaag ctcaacgcaa acaacagcgt atgaaccagc
    12061 aggagcaaaa acgaaagaac attgctcaaa agcgtttgga gctggataaa gcaaaagaaa
    12121 aaagacgaaa agaaaaccca tcatctcctg ttcagaataa ccagcagcat gttcgcaagc
    12181 gtcagcaaac cgtcacgcct gttttgtcac aaaacactaa acgtgaaaat catcagcgac
    12241 cattgaaaaa agagcaaaaa caaaagacac caccgaagca attcaatcag aggaagctaa
    12301 ataaaagaac ggataagcga ggacaacaaa catgaagcta aaaataacag tgatttgtag
    12361 tgtggtcttc tctctcttct tttttctctt attattccta gtcattttct ttgcagatga
    12421 tacggacagt ggcgaaaaca acaaggattc ttctattcca caaggcggtg tgaccgtttc
    12481 accagaagtg ttggcacacc gtccgttgat tgaaaaatat ggtaaggaat atgggattga
    12541 agactatgtt tcttatatct tagccattat gcaagtggaa tcgggcggta cagcagaaga
    12601 tgttatgcaa agttccgaat ctttgggttt accgcccaac agtttgagta ccgaagagtc
    12661 catcaaacaa ggggttaaat atttcagtga attattaacc agtgcagaac aacaaggagt
    12721 ggatatagat tctgttatcc aatcttataa ttatggcggt ggctttttaa attatgtgag
    12781 gagtcatggg aaaaaatata cctatgaatt agccgaacag ttttctaaag agaagtcggg
    12841 tggtcaaaaa gcagactatc ccaatcctat agccatacct gtgaatggtg gttggcgata
    12901 taactatgga aatcagtttt atgttcagtt ggtttcacaa tacctaaccg acacttcccc
    12961 aacagaattt gatgatgaaa ccgtgcaagt cattatggac gaagccttga aatacgaagg
    13021 gtttccctat gtctttggag gggcgtcgcc taccacctct tttgattgta gtggcttaat
    13081 acaatgggtg tatgacaaag ctggcatttc tctccctcgt gtcgcccaag atcagtatga
    13141 cgctacgcaa gaaatctcta tggaagaagc ccaagcaggc gaccttatct tctttcattc
    13201 aacctataat gcaggcacat atgtgactca tgtggcgata tatttagagg gaaatcgctt
    13261 ttatcatgca ggcgacccaa tcggttatgg cgacttaagc agtcgttatt ggcaagacca
    13321 tctgattggg gctagacgtg ttattcataa ctaagaaagg aatgaatcaa atgattataa
    13381 aaatcgagcg aaaagaaaag aaggagaaac caaagaaatc taaaaaaccg agcaagcaaa
    13441 gaaaagttcc gatgattaaa gttggcacgc ataagaaatt aacctttgtt ttatgggttt
    13501 tattgattgg tagtgtcgga tttggaatct ataaaaattt tactgccatt gacactcata
    13561 ctgtccgtga aacagaaatc attaaacagc agattgtcga taccaaccaa gtagaaagtt
    13621 ttgtgaaatc ctttgcgaaa gactactttt cgtggcaaca atcccaagaa gccattgata
    13681 aacgaaatga aaaattgacc cattatttga cggaagaatt gcaggtatta aatgaagaaa
    13741 tgattcgtaa ggatattcct actagttcta gtgtgaacga tatacaggtg tggcaggttt
    13801 ctcaagtgaa tgagaacacc tttgaggtac tgttttctgt tgaacaagtt attaccgaag
    13861 acaaagacaa ggaaactata tcgtctagct ttcatgtggt tgtccatata gatgaatcag
    13921 ataatatggt tattatcaaa aatccgacaa tgagtaagaa acctcaaaaa tcggactacc
    13981 aacccaaaca gctagaaagt gaccatacag tagatacaga aacgatggac gaaatcacta
    14041 gctttttaga aacgttcttc cagctctatc ctaccgccac agaaaaagaa ttgacttact
    14101 atgtaagtaa tcatgtgtta cctatgatta acaaggaata tgttttcgag gaattggtga
    14161 atcctatctt taccagaaaa gataaccaag taatcgtgaa tgtcgctgtg aaatatttag
    14221 atcaagaaac aaaggcaacc caaatctcgc aatttgagtt gatattagaa aaacaagata
    14281 attggaagat tgtaaaataa caaatattgg tacatgatta cagatacttt gtaatcatgt
    14341 actctttttg ataaaaaatt ggagattcct ttacaaatat gctcttacgt gctattattt
    14401 aagtgactat ttaaaaggag ttaataaata tgcggcaagg tattcttaaa taaactgtca
    14461 atttgatagc gggaacaaat aattagatgt ccttttttag gagggcttag ttttttgtac
    14521 ccagtttaag aataccttta tcatgtgatt ctaaagtatc cagagaatat ctgtatgctt
    14581 tgtataccta tggttatgca taaaaatccc agtgataaaa g
//
LOCUS       DAIGOK010000067        14588 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.067, whole genome shotgun sequence.
ACCESSION   DAIGOK010000067 DAIGOK010000000
VERSION     DAIGOK010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.067"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            30..842
                     /locus_tag="NOA01_001663"
     CDS             30..842
                     /locus_tag="NOA01_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HCI0632855.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            839..1639
                     /locus_tag="NOA01_001664"
     CDS             839..1639
                     /locus_tag="NOA01_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCI0632856.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1800..3068
                     /gene="rseP"
                     /locus_tag="NOA01_001665"
     CDS             1800..3068
                     /gene="rseP"
                     /locus_tag="NOA01_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HCI0632857.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3136..4845
                     /locus_tag="NOA01_001666"
     CDS             3136..4845
                     /locus_tag="NOA01_001666"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCI0632858.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5057..9409
                     /locus_tag="NOA01_001667"
     CDS             5057..9409
                     /locus_tag="NOA01_001667"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HCI0632859.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GNTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9553..10026
                     /gene="rimP"
                     /locus_tag="NOA01_001668"
     CDS             9553..10026
                     /gene="rimP"
                     /locus_tag="NOA01_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCI0632860.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10050..11225
                     /gene="nusA"
                     /locus_tag="NOA01_001669"
     CDS             10050..11225
                     /gene="nusA"
                     /locus_tag="NOA01_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCI0632861.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11247..11540
                     /locus_tag="NOA01_001670"
     CDS             11247..11540
                     /locus_tag="NOA01_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HCI0632862.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11537..11848
                     /locus_tag="NOA01_001671"
     CDS             11537..11848
                     /locus_tag="NOA01_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HCI0632863.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11861..14167
                     /gene="infB"
                     /locus_tag="NOA01_001672"
     CDS             11861..14167
                     /gene="infB"
                     /locus_tag="NOA01_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCI0632864.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14194..14541
                     /gene="rbfA"
                     /locus_tag="NOA01_001673"
     CDS             14194..14541
                     /gene="rbfA"
                     /locus_tag="NOA01_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCI0632865.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
BASE COUNT         4974 a         2577 c         3254 g         3783 t
ORIGIN      
        1 ggaatctttt aagtatagga ggaaaaagaa tgttacgttt ttttccgcaa aaaaacaaat
       61 atattcaaga aaaaagcgaa tttcaattta ataccgatgg accaattcct cagcacattg
      121 ccatcatcat ggacggcaat ggtcgttggg cgcaaaaccg tcgtttgcca cgagttgctg
      181 gccataaaga aggcatggaa acagtaaaga aagtcacaaa aaaagcatca aggcttggag
      241 tgaaagtgtt gacgctttat gccttttcga ctgaaaactg gaagcgacca aaagatgaag
      301 taagttttct catgcaacta cctgtcgact tctttgatac gttcgtacca gaattgatta
      361 aagaaaatgt caaagttcat gtcatgggtt atgaaaacgt attgccagaa catacgcaag
      421 atgctgtaag acgtgcaatt gagcaaacaa aaaacaatac tggaatggta ttgaattttg
      481 ctttgaatta tggaagtaga gcagagatcg tgactgctgt ccaagaaatt gccgaagaag
      541 tagcaaaagg agaaatacac gcagaagaaa tcgatgatga attgattgct aagcatttaa
      601 tgacaggttt cttgcctaaa gagttacaag atcctgagct gatgatccgt acaagtggag
      661 aagagcgaat tagtaatttt ctgttatggc agatcgcata tagtgagctg tattttacaa
      721 aagcgctttg gcctgatttt gatggtgcgc atcttgaaga agcaatcgcc tcttatcaaa
      781 atcgagaccg tcgattcggc ggtgtaaaaa aaacagaaga tgagaaaggg gatcagtcat
      841 gaaacaacgt gtgatcacag cagctgtggc gctagtcttg tttattccga ttatatggat
      901 cggaggcata gcggttgagc ttacagcagc aatcttagcg gtagtaggag tttacgagct
      961 gttccggatg aaaggattga cactcttaag ttttgaagga gttttatccg ccgttggtgc
     1021 agtgttactt gttttgccaa aagaaagatg gtttttcttt cttccagaga atgtaagtac
     1081 atttatgtta ttttatctta tagttatgat tcttttagga acatcagtta tttctaaaaa
     1141 tacgtatacg attgatgaag caggttttcc tgtgcttgtc agcttgtatg tgggaatagg
     1201 tttccagaat tttgtcaatg cacgaacaga aagcttggct gttttgatat ttggtctatt
     1261 tattgtttgg gcaactgata tcggagcata tatggttggt cgacgttatg gtcagcataa
     1321 gttaagtcct gatatttcgc ccaacaaaac gattgaaggt gcgctcggtg gtatcggtag
     1381 tgctgttgta gtcgcatttc tttatttatt gctataccca gcaaaagaac tattcaatca
     1441 tggtatgttt gtcatgcttc tgatgacgat tattttttca gcagtcggac aatttggtga
     1501 tttggtcgaa tctgcaatca aacgtcatta tgatgtgaaa gactcgggaa atatcttacc
     1561 aggccatggt ggaatcttag accgtttcga cagtttgctg tttgtatttc ctatcatgca
     1621 tttatttggc attatttagt aagtatacct gcgtttcttt gtgaatttca taaaagacgc
     1681 aggtttttat ttttcattat attgatacta ttggtgtctt tggtatacta tggtaaaatt
     1741 atagtgtttt atcgttgttt tataaaaaac acaaaaaaca agtaaaggag aaaaaagcta
     1801 tgaaaacgat tctgacattt atcatcgttt ttggtatatt agtgattgtt catgagtttg
     1861 gtcatttctt ctttgcgaaa cgatcaggaa tcctcgtccg cgaatttgct atcggtatgg
     1921 gtccgaaaat ttatgggcat caagcaaaag acgggacaac atatacgctt cgcctattac
     1981 ctattggcgg ttatgttcga atggcaggca atggggatga tgaaacagaa atggcaccag
     2041 gaatgcctct ttctctttta ctaaattctg acggcatcgt agagaagata aatttaagta
     2101 agaaaatcca attgacaaat gctatcccga tggaattgag ccgttatgat ttagaagatg
     2161 agctgactat cactggttat gtgaatggag acgaaacaga agtagtaacc taccctgttg
     2221 atcacgatgc gacgattatt gaaaacgacg gaacagaaat cagaattgcg ccaaaagacg
     2281 tacagttcca atcggctaaa ttgtggcagc gtatgctgac caactttgct ggacccatga
     2341 ataactttat tttggcaatc gtgttattca tcattctggc atttatgcaa ggtggtgttc
     2401 aagtgacgaa cactaatcgc gtaggagaga tcatgcctaa tggagctgcg gcagaagcag
     2461 gcttaaaaga aaatgatgag gtagtcagtg tggacggaaa agaaatccat tcatggaatg
     2521 atctgacaac agtcattacg aaaaatccag gtaaaacatt agactttaag atcgaacgag
     2581 aaggtcaggt gcaatctgtc gacgtgacac cgaaatctgt agagtcaaat ggcgaaaaag
     2641 ttggccaact tggtataaaa gccccaatga atacaggttt tatggataag atcattggag
     2701 ggacgcgtca agctttcagc ggttcattgg aaattttcaa agccttagga tcactattta
     2761 caggtttcag tttagataaa ttaggcggac ctgtcatgat gtaccaattg tcttcagaag
     2821 cagcaaatca aggtgtaaca actgttatta gtttgatggc gcttctttca atgaatctcg
     2881 gaatcgtcaa tctgcttccg attcctgcct tagatggcgg gaaattagtc ttgaatattt
     2941 ttgaagggat tcgcgggaaa ccattaagtc aagaaaaaga aggaatattg acattagctg
     3001 gctttggatt tttaatgttg ttgatggtgc tagttacatg gaacgatatt caacgattct
     3061 ttttctagaa aaagttgaca aatagggtat aataacaaac gataactatt ttgaggaaat
     3121 aaaggagtat atagaatgag acaatcaaaa atgttgattc caacattacg cgaagttcca
     3181 aatgatgcag aagtattaag ccatcaaatc ttgctaagag ctggatatat ccgccaagta
     3241 tcagcgggta tttattcata tctgccatta gcaaatcgtg tattggaaaa actgaaacgc
     3301 attatgcggg aagaatttga aaaaatcgga gcggtagaaa tgctgatgcc tgccatcttg
     3361 ccagcagaac tttgggaaga atccgggcgt tacgaaacat atggaccaaa cttgtatcgt
     3421 ttcaaagacc gcaatgatcg aaaaatgatc cttggaccaa ctcacgaaga aacttttact
     3481 gatttgatgc gtaatgaaat caattcatat aagaaattac ctttaaatct ttatcaaatc
     3541 caacctaaat atcgtgacga aaaacgacct cgcttcggtt tgcttcgcgg aagagagttt
     3601 atcatgaaag atgcatattc tttccatgca acggaagaaa gtttggacga gacatataaa
     3661 gattacgaaa aagcctatac cgaagtattc aagcgttgtg gcttagaatt tcgctcgatc
     3721 atcggagacg gcggcgcaat gggcgggaaa gactcaaagg aattcatggc gatctcagaa
     3781 atcggggagg atacgatctg ttattcgact gaaagtgatt atgctgcaaa cttggagatg
     3841 gctacaagct actatgtatc aaaaaaatcg catgaaacac agcttgaatt ggagaaagta
     3901 gctacaccta acgtaaaatc aattgacgaa gtagcagatt tcttttctgt tgaacctcaa
     3961 aaaatcatca agtctgtatt gttcattgcg gatgaacaac ctgtccttgt tttggtacgt
     4021 ggagaccatg aagtcaatga cgtaaaactg aaaaactttt tatctgctga ctttttagaa
     4081 gaagcaacag aagaagaagc acaaaaatat ctaggtgctg agtttggttc tgtcggacca
     4141 gtcggtgttt cagatgacgt aaaagtatat gcagaccgtt atgtccaaga tttagcgaat
     4201 gccattactg gagcgaacga aacaggattc cactataaaa atgtgaatcc aaaacgcgat
     4261 ttcactgtgc tttcttatga agatcttcgt tttgtacaag aaggagatcc ttcaccagac
     4321 aacaacggtg ttcttgcctt tactcgagga atcgaaatcg gacatatatt caaaatcggc
     4381 actcgttaca gtgaatctat gggagcaact gtactagacg aaaatggacg agaaaaattt
     4441 gtgattatgg gctgttatgg tatcggtgta agtcgtttat tatcagcaat cgtagaacaa
     4501 cattcagatg aacaaggaat caattggcca aaaggaattg cgccatttga tctgcatatc
     4561 gtccaaatga atctaaaaga cgaataccaa acaagcctga cgaatgaact agaagaagcg
     4621 atgacaaaag ctggctatga agtactggtt gatgacagaa atgaacgcgc aggagtgaaa
     4681 ttcgcggatt ctgatttgat tggctgtcca atcagaatca ctgtcggtaa aaaagcagtg
     4741 gatggaatcg tagaagtgaa aatcaagaaa actggcgaaa tggttgaagt acgtaaagaa
     4801 gaattggcga atactttacc aatcctcttg aaccaaggcg aataataagc gttaaaacac
     4861 agaaaataaa aatagctgaa tcagctgttt ttgacaatct gatgattcgc ttttaaaatt
     4921 ggaactgcga tttgaatcgc agttccaatt ttttaatgta aaactcagaa aaaacgagta
     4981 tactaaaagg tactgaaaaa gaagaggata agatgcgatc aattgcattg tttctcatca
     5041 gaaagggcgt atatctttgt cagaaaaacg agatttattt gaaaaactgt tggaacaaat
     5101 ccaactagag gaagcagaaa aaaatcatcc actgcttact gctggtgaaa tggatagtgt
     5161 agtggtccat cgtaaatcgc gtttatggga atttacgcta catttttcca aaattttgcc
     5221 tattatgcta tatcggtcat tgactcagca tctgactatc gcttttaaag atattgcaca
     5281 agtcaaactg aatatcttag cagatgacca gacattcgat gaacagctct tgcaagatta
     5341 ctgggcccaa gctttggaaa atcagcaatg cgatacacca ctagcacagc aagtattgaa
     5401 aacgcaggta cctgtgataa aagatcgtaa agtcatcttg ccaatcgaca gtacaggagc
     5461 tatttcttat ttgaagcaac aatacttgcc attagtagaa gagttgttcg tcagttacgg
     5521 attcccaaaa tttcgtatcg aaccagaagt ggatgagcag caagcggaac gtgtattgaa
     5581 actttttgaa gaacgcaaac aagaacaggc agaagccttt atgaagcaag cagctgaaag
     5641 tctagttgtc cacgaacaaa agaaaaagga aagaaaagag caatcgccag ctcttgaagg
     5701 acccatccag ctaggacgaa atattccagc cgatgaacca acgacaccaa tgatcagtat
     5761 cgttgaagaa gaacgaagag tcacacttga ggggtacgta ttcgacaaag aagtcagaga
     5821 actacgttca aaacgcaaga tcttgacttt gaaaatcaca gattatactt catcatttat
     5881 cgtcaaaaaa ttctcgaacg gcgaaaaaga tgaacaagta tttgatgcga tcagtgtcgg
     5941 tagctggtta aaggtacgag gaagtattca agaagatacg tttgtccgtg atttggtcat
     6001 gaatgcgcaa gatatcatag aagtcaaaca tacacctcgc aaagattatg ctcctgaagg
     6061 ggaaaaacga gtagagcttc atgttcatag caatatgagt acgatggatg caacaaacag
     6121 catctctgat cttgtggcac aagcaggaaa atggggacac cgagctatcg ccattacaga
     6181 ccatggtggt gcgcaagcct ttccggaagc acatagtgct ggaaaaaaag caggtgtgaa
     6241 aatcttatac ggcgtggaag cgaatgtcgt agatgatggt gtcccaattg cttataatga
     6301 tgcacatgaa gcattgagtg aagccacata cgtcgtattt gacgtggaaa cgacaggatt
     6361 gtctgcagta tatgacacga tcatcgagct agccgcagtc aaaatgtata aaggaaatgt
     6421 gatcgaaagc tttgatgaat ttatcgatcc aggacatcca ttgtcacgaa caacggtaga
     6481 tctgactggt atcacggatg aaatggttcg agggtcaaag tcggaagaag aagtattgcg
     6541 gatgttcctt gaattttcaa aagatacaat tttggtagcc cacaacgcgg cttttgatat
     6601 ggggttcttg aatactagct acgcaaggta tggtattcct gaagcggcca atccagtcat
     6661 tgatacgttg gaattagcta gatatctgta tcctcaattc aaacgtttcg gattaggtgt
     6721 gttatctaaa aagtttggtg ttagcttgga acagcatcac cgagcaatct atgatgctga
     6781 agcaactggt cacttagcat ggatctttgt gaaagaggca atggacaatc ataacatgct
     6841 ttatcatgat cagctaaatg agcatatcgg agaaggagat tcttacaaaa gggctcgacc
     6901 tttccatgta acgattttag caaaaaatca ggcaggactg aaagatttgt tcaaactgat
     6961 ttcaatgtcg aatgtcgaat acttcgaacg cgtcccacga ataccacgct cccaattgaa
     7021 aaagatgcgt gaaaatctat tgatcggctc agcttgtgat aagggagaaa tattcgaagc
     7081 catgatgcaa aaaggggtag aagaggcaag aaaccgagca aaattctatg attatattga
     7141 agttatgcca aaagcggtct atgcgccttt gatcgagcaa gaattggtaa aaaatgaaca
     7201 tgatttggaa gaaattatcc aaaatttggt agaaatcgga aaaagtttgg ataaaatcgt
     7261 tgttgcaaca ggtaatgtac attatctgaa cgaagaagat gccatttacc gaaaaatttt
     7321 gatcaattct atgggcggag cgaatccgtt gaatcgtcat agtttgccgg atgtccattt
     7381 ccgaacaacc gatgagatgc taactgcatt tcacttttta ggggaagaaa cagcgaaaga
     7441 gatcgtagta gaaaatacga ataaaatcgc agatatctgc gaagaagtga ttccagtcaa
     7501 agatgaactt tatacaccga agattccagg ttcagaagat gaaatatcag aattgagtta
     7561 cacaaaagca aaacaaatgt acggagatcc gttacctgag atcattcaaa aacgattaga
     7621 aaaagagttg aattctatta atggtaatgg cttctcggtt atttatctga tcgcccaaaa
     7681 actagtgcat aaaagtaatg aagatggcta tctagtaggt tcacgtggct ctgttggctc
     7741 tagctttgtt gcgacgatga caggaatcac tgaagttaat ccattagccc ctcattatta
     7801 ttgtccagag tgccaatatt ccgagttttt tgaagatggt acgtatggtt ctggatttga
     7861 catgccagaa aaacaatgtc caaaatgtgg tgcacgctta aacaaagacg gtcatgatat
     7921 tccatttgag acatttttag gattccatgg agacaaagtg cccgatattg atttgaactt
     7981 ctcaggtgat tatcaagcag aagcccataa ctatacgaaa gtattgtttg gcgaagacta
     8041 tgtttaccgt gccggaacga ttggtacagt cgccgacaaa acagcatatg gctatgtaaa
     8101 aggatacgaa agggacaata atctgcagtt tcgaagtgca gaagtcgaca gattagcaaa
     8161 aggcgctact ggagtcaaac ggacaaccgg ccagcatcca ggggggatca tcgttattcc
     8221 cgattatatg gatgtctatg attttacgcc gatccaatat ccagcagacg atcaaaattc
     8281 agaatggaaa acgactcatt ttgatttcca ctcgattcac gataatgtat tgaaattaga
     8341 tattctaggg catgatgacc caactgttat tcggatgctt caagatttat cagggatcga
     8401 cccacaaacg atccctacgg atgacccgga agtgatgcgg atatttgccg gtcctgaagt
     8461 acttggagta agtcaggaac aaatttattc caaaacaggg acattaggta ttccagaatt
     8521 cggcactcgt tttgttcgag ggatgttaga agaaacgcat cctacaactt ttgcagaact
     8581 actgcaaatt tccggactat ctcatggtac agacgtatgg ttaggaaatg cggaagaatt
     8641 gattcgacga ggagatgcga cactggcgga agtaatcggc tgtcgtgatg atattatggt
     8701 ttatttgatc catgctggat tagatagcgg gatggcattt aaaatcatgg aaactgttcg
     8761 taaaggacaa tggaacaaga tccctgatga attgcgagaa acctatctat cggcgatgaa
     8821 agaaaataat gtgcccgact ggtatatcga ttcttgttca aaaatcaaat atatgtttcc
     8881 gaaagcccat gctgcagctt acgtattgat ggctcttcgt gtcgcttatt tcaaggttta
     8941 tttccctatt ttgtattact gtgcatactt ttctgtacgt gcagacgact ttgatctagt
     9001 ctccatgtgc aaaggaaaag atgctgtgaa acaagcaatg aaagaaatca cggacaaggg
     9061 attagatgct tccgtaaaag aaaagaatca actgacagtg ctggagttag ccaatgaaat
     9121 gctggaacga ggattcaaat ttggaatgat tgatttgtat aaatcggatg ccgttaactt
     9181 tgttattgaa ggtaatacgt tgatcgcacc atttagagct gtcccaagtt tgggaaccaa
     9241 cgtggcgaaa caaatcgttg aagcaagaaa agacggtcca tttttatcca aagaagatct
     9301 tgccacccgc ggtaaagttt caaaaacatt gatcgaatat atgaacgaca atggcgtatt
     9361 gaaagatcta ccagatgaaa atcaattatc gttattcgat atgctataag catctgtttt
     9421 cctgtctgct tgtaatttta agcagagtat ggtatagttc ttataggtaa tgatactaac
     9481 tgtgagtgag cggatttttc gctcactctt tttaatggaa ttacgcactt aatttttaag
     9541 gaggcgaaac ttttgagtag cgtcgttgaa acagtcacag aaatggtgac accgatttta
     9601 gatgaacaaa attttgaact tgtcgaagtc gaatttgtaa aagaaggaaa aagctggttc
     9661 ttacgagtat ttattgacaa agaaggcggt atcgatatcg aagaatgcgc ctttgtcagt
     9721 gagaaactga gtgaaaaact tgatgcagca gatccagatc cgattcctca agcttatttc
     9781 ctggaggtat cctccccggg agcagagcgg ccattgaaaa aagaaagcga ctatgaaaag
     9841 gcacttggtg attatgtcca tgtctcctta taccaaccag tagatggcga aaaacaatat
     9901 gaaggatttc ttcaatcatt tgatgctgaa cagcttacat tgaaaatacg cataaaaaca
     9961 cgagaaaaag aattgacttt tgaccgaaaa aatattgcaa aagctcgctt agctatccaa
    10021 ttttaatcac ggagaggaat cagaaaaaaa tgagtaaaga aatgttaaat gcgttagacg
    10081 ctttagaagc tgaaaaaggg atctcaaaag aaatcgtgat cgatgcttta gaagcagcat
    10141 tggtttctgc atacaaacgt cactatggcc aagcacaaaa cgtcgaagtg gaatttgatc
    10201 agaaaaaagg aaagatccat gtctatgcgg taaaagaagt aacggaagaa gtgatggatt
    10261 cacagttgga agtctcattg aaagatgcac ttttaatcaa tccagcatat gagatcggtg
    10321 atacgatccg ttttgaagta acaccaaaag attttggacg gatcgctgcg caaacggcta
    10381 aacaagttat tttacagcgt gtacgtgaag cagaaagaac gattatttat aacgaattca
    10441 gtgcttatga aaaagacatc atgcaaggaa tcgtcgaacg ccaggataaa cgttatattt
    10501 atgtcaatct tggtaaaatt gaggcagttc tttctaaaca agatcaaatg ccaaatgaat
    10561 tttatcaacc acatgatcga atcaaagttt atgtttcccg agtagaaaat acgtctaaag
    10621 gtccgcaagt atttgttagc cgcagccatc cagacctatt gcgtcgctta tttgaacaag
    10681 aagtaccaga agtctatgat ggaatcgttg aaattgtcag catcgcccgt gaagctggcg
    10741 atcgttcaaa agtggcagtt cgctcaaatg atccaaatat cgatgcagtc ggtacatgtg
    10801 tcgggccaaa gggacaacga gttcaagcaa tcgtcaatga attaaaaggc gaaaacatgg
    10861 atatcgtcga atggaatgaa gatccagctg tattcatcgc aaatgcattg aatcctgcac
    10921 aagtcatgga tgtgatcttc gatgagacaa atccaaaggc ctgcacagtt gtcgttccag
    10981 actaccaatt atcgctagca atcggtaaac gcggacaaaa tgcacgttta gctgctaaat
    11041 tgactaacca taaaatcgat atcaaatcag aatctgacat gacggaattt tatgaaaatc
    11101 aagaacaaca aaaagaaaca gaagaactgc atgatgaagc aatcgttcag tcagatttaa
    11161 cagacgatga atatgaaaca attgcattca acaatgaaac ggtcgaagaa aaaccagaag
    11221 cttaacttta gtaaggaggc aaagagatga aacaaaggaa aattccttta cgcaaatccg
    11281 tggtttcagg tgaaatgaaa cctaaaaaag agatggtccg aatcacccgt tcaaaagaag
    11341 gggaagtatc gatcgatcca actggaaaaa tgcctggacg aggagcgtat gtctcacttg
    11401 agccggaaga agtacaacaa gcttgggata aaaaaattct cgatcgagta ctcgaaacaa
    11461 cattaacaga tgagttttat caagaacttc ttgactatgt cactcaccaa aaagcacgta
    11521 aagagttatt tggaaaatga atacagttaa tcggcaaaaa gccatgaatc tcatcggtct
    11581 agcgatgcgc gcgggaaaac taatcacggg tgaagaactg accattgctg aaatccgcaa
    11641 acaaaaagca aaattggtgt ttgtggcaac cgatgcaagt gagaatacaa gaaaaaagat
    11701 aaaagataag agttcatatt atgaagttcc ttgctttgag ctattttctg aagcagaaat
    11761 cacgcagatg atcggaaaac ctcgcaaggt attcggaata atggatgccg gatttgcgaa
    11821 gaagaccaag gagctaattg aaggttagga aggtgattgc atgggcaaca aaagaatcta
    11881 tgaattagca aaagaactca aaaaatctag taaagacgtc gtggaaaaag cacaacaact
    11941 tggcatcgat gtgaaaaacc atatgggtgc gattactagc agtgatgaga agaaattgca
    12001 acaagcgttc aaaaacccaa gcacgaaaca acaagctgca caacaagcaa gcagaaaacc
    12061 agccaatcaa cagccgaacg atcaacgtaa aaaagaaaca acgaacaatc aacaaaaaaa
    12121 taaaaataac cgcagttatc aagatcgcgg tcaagggagc ggtcaagtga atcaaggaaa
    12181 aaaaccaaca acaaaccaaa ataatacggg aaataccagc agccaaaatc gtcaaggaac
    12241 gagccaaggc ggcaatcaaa accgtactaa caataacaac aaaccaaaca ataacaatcg
    12301 cggcaaattt aataataata accgtaatcg ttataataaa aaaggtaaaa aaggcaaaca
    12361 gcaacaatca actaaaccag ctgtaccacc acgcaaattc cgtgaattgc cagatgtatt
    12421 agagtatact gaaggtatga acgtagcaga tattgcgaaa aaaattcatc gtgagccagc
    12481 agaaatcatt aaaaaattat ttatgcttgg tgtcatggtc aatcaaaacc aagcactgga
    12541 taaagatacg attgaactgt tagcgacgga ctatggcatg gaaccacaag aaaaagtcca
    12601 agttgacatt gctgatatcg ataaattctt cgaggcagaa gaagtcgatc cagataaatt
    12661 agtctcacgt ccgccagtag taacgattat gggacacgta gaccacggga aaacaacctt
    12721 attggataca ttgcgccatt cacgtgtaac aagcggcgaa gccggcggaa tcacacagca
    12781 tatcggtgcc taccagatcg atattgatgg taaaccaatc actttcttgg atacaccagg
    12841 acatgcggcc tttacaagta tgcgtgcacg tggagcaagt atcacagata tcacgatctt
    12901 agttgttgct gcagatgatg gcgtcatgcc acaaacagtt gaagcaatca accatgccaa
    12961 agctgcaggc gttccaatca tcgtcgctgt aaataaaatt gataaaccag gtgcaaatcc
    13021 tcagcatgtt atgcaagaat taagtgagta tgaattaatt cctgaagcat ggggtggaga
    13081 aacgattttt gttgagatct cagcgaaatt cggccaaaac atcgaagaat tgctggaaat
    13141 gatcctgtta gtcgcagaag tagaagattt aaaagcagat ccaacacaac gcgcaatcgg
    13201 aacagtgatc gaagcacgtc ttgataaagg taaaggtcct gtgtcaacct tacttgtcca
    13261 agaaggaaca cttcacgttg gtgacccaat cgttgtcggt aatacttacg gacgtgtccg
    13321 tgtaatgaca aacgatcttg gccgtcgcga taaagaagca ggacctgcaa cgccagtaga
    13381 aatcactggc ttgaatgatg tacctcaagc aggtgaccgt ttcgtagtct tcgaagacga
    13441 aaagacagca cgtgcagcgg gtgaagaacg cgcaaaacgt gcattattgg aaaaccgtgc
    13501 tgccacaagc cgtgtgactt tggacaactt atttgaaagc ctgaaagaag gcgaattgaa
    13561 agaagttaac gtaatcatca aagcagatgt acaaggttca gcagaagcac tggctgcatc
    13621 attgaagaag attgatgtgg aaggtgtccg tgtaaaaatc gtccattcag ctgtcggagc
    13681 aatcaatgaa agtgatgtaa ccttagcagc agccagcaac gctattatca tcggtttcaa
    13741 cgttcgtcct acaccgcagg caaaagtgca agctgatact gaagaagtcg atattcgtct
    13801 tcaccgtatc atctataaag ccatcgaaga aatcgaaact gcgatgaaag ggatgctaga
    13861 ccctgaattt gaagaaaaaa tcaccggtca aatgatcgtt cgtgaaacct tcaaagtttc
    13921 taaagttggt acgattgcag gtgcctatgt aacagaagga tatattcgta gagatagcgg
    13981 tgtccgtgta atccgtgacg gaatcgtaat ctatgaaggc caacttgcaa gtctgaaacg
    14041 attcaaagat gacgtgaaag aagtcaaaat gggttatgaa tgtggtgcga tgattgaaaa
    14101 attcaatgac atcaaagtag atgacgtaat tgaaggattt atcatggaag aagtcaaaaa
    14161 tgactaaaaa ctgataaaag gaggccaatc attatggcta actaccgtga tcgccgagtc
    14221 ggtcaagaaa tcttaaaaga agtcaatgat attttgcgca aaaaggtccg tgaccctcgt
    14281 gtggagaacg taacgatcac agatgtgaaa gttacggggg acttacagca agcaacgatc
    14341 tattacagca ttttatctga cttagcatca gataagaaaa aagcacaaga tggattgaat
    14401 aaagcatcag gattgattcg tcgagagtta gggcacaatc taagtattta taagacacct
    14461 gaattgttct ttgaacttga tgaatcagtt gtctatggga accatatcga tgaattgctt
    14521 cgcgacttga acaaagacta atcaaaaaat cccgcaaatc ctgtgaaatc aggggttgcg
    14581 ggattttt
//
LOCUS       DAIGOK010000068        14402 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.068, whole genome shotgun sequence.
ACCESSION   DAIGOK010000068 DAIGOK010000000
VERSION     DAIGOK010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.068"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            15..1355
                     /locus_tag="NOA01_001674"
     CDS             15..1355
                     /locus_tag="NOA01_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCI0632866.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            1573..2016
                     /locus_tag="NOA01_001675"
     CDS             1573..2016
                     /locus_tag="NOA01_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HCI0632867.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(2520..3938)
                     /gene="nhaC"
                     /locus_tag="NOA01_001676"
     CDS             complement(2520..3938)
                     /gene="nhaC"
                     /locus_tag="NOA01_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCI0632868.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(4341..5564)
                     /locus_tag="NOA01_001677"
     CDS             complement(4341..5564)
                     /locus_tag="NOA01_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HCI0632869.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(5595..6434)
                     /locus_tag="NOA01_001678"
     CDS             complement(5595..6434)
                     /locus_tag="NOA01_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCI0632870.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(6451..7131)
                     /locus_tag="NOA01_001679"
     CDS             complement(6451..7131)
                     /locus_tag="NOA01_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI0632871.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(7133..8206)
                     /locus_tag="NOA01_001680"
     CDS             complement(7133..8206)
                     /locus_tag="NOA01_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCI0632872.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(8262..8500)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(8543..8776)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8853..9524)
                     /locus_tag="NOA01_001681"
     CDS             complement(8853..9524)
                     /locus_tag="NOA01_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCI0632873.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(9611..12289)
                     /gene="mgtA"
                     /locus_tag="NOA01_001682"
     CDS             complement(9611..12289)
                     /gene="mgtA"
                     /locus_tag="NOA01_001682"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCI0632874.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            12948..13385
                     /gene="ndk"
                     /locus_tag="NOA01_001683"
     CDS             12948..13385
                     /gene="ndk"
                     /locus_tag="NOA01_001683"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCI0632875.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(13407..14273)
                     /locus_tag="NOA01_001684"
     CDS             complement(13407..14273)
                     /locus_tag="NOA01_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCI0632876.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
BASE COUNT         4139 a         3011 c         2544 g         4708 t
ORIGIN      
        1 gaaagaggtc atttatggaa gataaaaaat tgaaaaaaca acttagctcc agacatatca
       61 ccatgttagc tttaggtgga gcaatcggtg caggtctatt taaaggaagt ggcgaagcga
      121 ttgggatcgc cggcccttct gtattgattg cttttttgat tggtgggctg attttatata
      181 tcgtgatgaa aggtctggga aaaatcgttt taagcggtgg cgacacgcat cacggattat
      241 ctggtttgat acgcccttat ctaggtgctc attctgctga ttttactgat tgggtctact
      301 ggtctatgtg gattatcaat atcatcgctg aagctgttgc agctgcttcc tttttgcagt
      361 tgtggttccc gcacattcca gcctgggtat ttgtttttat gttggctgtt ttaactacga
      421 tcatcaatct ttattcggta cggttattcg cagaaacaga atattggctg gcttttgcta
      481 aaatcagtgt cattatctta ctgatcattt ttgctactta tcttgtcggt cagcaaatgt
      541 tgggcacagg cgttttccct acccttcagc aattgactga ccatggtggg tttactcctc
      601 atggtatgaa gggaatcatc agttctttgc ttgtagttat ctattcttac ggcggttctg
      661 aactgattgc tatcacggta agcgaagcag atgatcctaa acgtgcgatt cctaaagcaa
      721 tcaaaggtgt catgggaaga atcatttcct tttacatcat tcctttgttt ttgcttttga
      781 ttctttttcc ttggaatact ttagcaggca cgaatgtcag tccttttgtg atggtatttg
      841 aaaaaatgaa tatcccattt gcagcggata tcgtcaattt tgtgattgtc cttgctttgt
      901 tctcatctat caattcgggt gtgtatgcat cttcaagaat tttgttcttc cgtttgaagg
      961 atcgaaaagg ttcctctaga aaactagctg ttttaaacaa gcatcaagta ccgcaacgtg
     1021 ctgttttatt ttgtaccagt gttctttatc ttggtgtagc tctttcttat tttgtcggtg
     1081 acaaattatt tggctatctg gcaggttccc tttcgtatac agtattattg atctggatca
     1141 ttatcagtgc ggcatccttt gtactggcac taagaaaagg aacattatgg gataaaggaa
     1201 tcagcttttt tgcattagct gtcttaggat tgattttcct tggtatcctg ttcacgaact
     1261 ccattggggt aactgtattg actggcctgc tttacctgtt catttatttc agttatcaaa
     1321 aaaagaatga tgcgttcgta ttgacgaatg aatgaaatcg atagaaaatt ttattttatt
     1381 tatgtggaat agatggagct attgaagaaa ggagttgtga caaaagtctt gttgcaactc
     1441 ctttttccga ataaacagtc tcatgacctc ttggtaaacg gtgttcaaaa accctctgtg
     1501 caaaataatt gattgttctc catgctcatg acatgataga agaaaggaat gaaaaaaagg
     1561 agtttgtttt aaatgaaaaa gtattggaag tttttgttga tgatcggtgt ttcaacggta
     1621 gttatgtttg gtatgatgta cttgaatgtc tatgcgctcg atcatatctt cttcagtcgt
     1681 acacgagtat tcatggcgtt gatgatgggt gcagtcatgg cagtcatcat gctgttgttc
     1741 atgtggaaaa tgtatgaaaa caaaaagata aatgttatga tcttagccgc tagtcttctt
     1801 gtttttgccg gttccttgtt tatggtacga agccaaacta ctgtcaacga cacagcttgg
     1861 atgaaggcca tgattcctca tcattctatc gctatcctgg caagtaaacg agctgattta
     1921 aaagatcctg aagtaaaaaa attagcggaa gaaatcattc aggcacaaga agaagagatc
     1981 gacaaaatga aaaaaatgat caaagaaatg gaatgaccat tatccgtcca aataaaaaaa
     2041 gaggatgttc cagaagttat cagcttcaag aaataagaag aagctacttc tggaacgccg
     2101 tgaataaggg gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa
     2161 tagcttctcc tatttttcgt ttggttaggc ttattaccgc agatgcaagc ttttgaacac
     2221 cgtttattaa gaggttgtaa aatcagcgtt ctgacctgag tattagagag acaaatggaa
     2281 aaatagttcc tcatattttg ccattttttg agctaatacc gagggtcagc tgattgaaca
     2341 ccgtttatcg agaggttgtg aaaaagtcgc ttagtttcgc gtattaagaa aaattttgtg
     2401 aaatggcttt ccacattttt gtataaattt ggcttaatac cgagaaactg gcttttgaat
     2461 accgtttatt cgtaaaaggg attgtgacaa aagtcttgtc acaatccctt tttatgtttt
     2521 tattgttcta attgcgctgt cttgcttact gcttcttgcg gtttcacaaa tttttttgag
     2581 attccccaac ctgtgaaggc actgaccatc gaaaacacag gagaaagcaa actgaaaaat
     2641 acgaatggca agtaagtgag tgtaggaata ccaaatgtac ttgctacgaa acttccggca
     2701 attccccaag gcactaagta gttgattact gttccaccat cttctagcgt tctacctaat
     2761 acagttggat caaatcctgc tttttcataa gtttctttaa atgcattacc aggcagaata
     2821 acagatagga attgttcacc aataaaaata tttactccga tacttgtcat caatgttgta
     2881 aaaatcaaat ttggtgtgtt cgtcatcttt cttgatactc gttccatcac ggttgagatc
     2941 aagccaaatt cgatcaacaa tccacctaat gacaaagtta gaataatcaa tgacacagta
     3001 ggcatcatac tttcgattcc tccgcggcta agcagtgaat cgatttgttg gtttccagta
     3061 tttgaaacaa accctgattc gatgacccca gccaacttcg ttaaggaagt ttgtgggttt
     3121 tgaataaaaa tcattcctac tgctacagcg atattcaaca aaatcgttaa aattgccggc
     3181 acctttttcc atgcacacag taacattaaa aataaaggaa tcagtgacca gattgaaata
     3241 gtaaagtttg cttctagaac atcagtcact tcacgaatag atgaaagact tgcggcttta
     3301 cttgtgtgtc ctaaaatcat gaataaaaag aaagaaacaa caaatgccgg gacagttgac
     3361 cacatcatat gcttgatgtg tttgaacaaa tcggcatcaa caatagctgc tgctagattt
     3421 gttgattcgg ataatggaga catcttatct ccaaaaacgg cacctgaaac aatagctcca
     3481 acaaccagcg ctggatttaa gcctagtgtt gtcccaatac cgaaaaatgc aatcccaatc
     3541 gttgacataa cagtaaaggc actccctaca gcgctaccta cgattgcaca cacaacaaat
     3601 acagaaggga caaaccactg gacactgatc aatttgaacc caatgaccat tagagttgga
     3661 atgatccctg cttgtatcca tacagcaatc aatgaaccga ctaaaataaa aataaagatt
     3721 gggatgattc ctggtttgat gccattgatg attccttcgt gcagccaatc tgtcgggtat
     3781 ctttttatca ctgcatacaa catgaccatt ccgatcgctg ctaagatcgt gatattaggt
     3841 gagaccttta atccgacaac gccaatcgca atgcctaaaa caataaccat taatacaagt
     3901 gttgcttcta aaaaacttgg tgttcctttt tttcccatga taatctctcc ttttttagtt
     3961 gttcttatgt tctaaaaaag ttaagatatc ggattactcc gccgcaattc atccgcgatt
     4021 cctcctttat aaaaataaaa agacctgttt taaactaagg acgaatgact ccgcggtacc
     4081 accttaatta aaaacagatc tgtttttcac tttcaaactt ttattcaaat gtatggactt
     4141 tccatgtaat tcgtcaatta ggcttgatcg gttcacagcg accaccgact ttctgaacaa
     4201 aacacctaac cgctactgcg tgaaaagttg acttgtacga agtttacctt ccataagatc
     4261 atttgtcaat gaaaaagaaa atattttttc atttttctga aaattatttt tataactttc
     4321 acacacaagt ttatttttta ctagaaagta gcgccctctt gtttttttct tgctactatt
     4381 tcttgatgtt cttgcttgat tctttctaat agctttggct ctttcaacag atcaagagct
     4441 gtattggcga gtagttctgc tgcaatcgca atagaagcaa ggcctttgtc acttttagca
     4501 gcagctttga attcttctgt gtgtccggca atatatgtct cagagattgc taccgtaggc
     4561 tggattgttg gtatgacttg gctgacattt cctacatcag aagaaccgag acttgttctt
     4621 tgttccgttt ctatttcttc atctggaaca cctgcttctt ttgcatgctg gaagaataag
     4681 tcatcaaaag aaggagtaac gatgacatca tcaactgcat tttggaacaa tccaaactca
     4741 aaagtcgtgc ccgtacttat cgcagcacct cgtacgatat tttccacttt ttcatatact
     4801 ttatctaatg tttgccgatt tcctgcacgt aaatagaaac gtccagcagc gtaatctgga
     4861 atgacgttgg cagctactcc tccgtctgta atgatcccat ggatattgac atcttttggc
     4921 aattgcaagc gtagagcgtt gattccatta aacacttgga tcaatgcttc caaagcattt
     4981 actccttttt caggtgctgc tgctgcatgg gaagcgacac cgtaaaattt gatgtcgact
     5041 ggatcattcg ctaaagattc tgtcgttttc ccataacgaa aagctggatg gacgcataac
     5101 gctacatcga catcctcaaa aaaaccttct cgaacaaagc tgcctttagc tgatccgttc
     5161 tcgccccctt cttcccctgg ggtgccatat acacggattt ctccaccagt ttcatcaatg
     5221 aattgcttca ctatgctggc ggctaatacg gagtaagtac caaacaaatt atgcccgcat
     5281 gcatgaccaa tacctggtaa agcatcgaat tctgccaaaa aggcaattgt gggaccgggt
     5341 ttattggaaa tataacgtgc atcaaaggct gttcggtgtc cagctacttc tttttctacc
     5401 caaaaccttt ctttttctaa ttgtttaatc aacgctgctg aggaataaaa ctcatagtta
     5461 cttacttcag gatgttcgtg gatatctaga gcaagtgctt gatattctgg taaacgatat
     5521 tggattagat ccttcaattg gtttgttgag attgttgttg tcataaaaaa ttcctcctat
     5581 gctagtcatt tttcttattt tctcccgaat gtttcccatg caggcacgct tgatcctttt
     5641 gatgttttct cgattacttt gactgtgtct tctgtttgat aagcgtctac tattttttga
     5701 tacgttttat tgttttcatc ttcttttctt gcaacgatga tattcacata aggacgtgca
     5761 tggtcatcca ctggttctaa gaagatggca tcagaagcag gattaaatcc agcatctaca
     5821 gctacaccgc tattgatcac tgaaatgtct acatctgaca aagcacgtgc tgtttgggaa
     5881 gcatctaatt cagaaatgtc tagatttttt ctattctcgg taatatcaga aacagtgggc
     5941 gcttgtcctt ttgccttatc cactttgatc aatccagcag tttgcaataa cagaagagct
     6001 ctgccgccat ttgtaacatc attagggatt gctacttttg ctccgtcttt gatatcagct
     6061 acatccttca aagtgttaga ataaattccc agcggtgcat tgacagtatt tccgatcgat
     6121 actaaatcag ttccgtgttc tttattgtaa ttgtctaaaa agaattgatg ttggaaagaa
     6181 ttcaaatcaa tcgatccatc tgctaaagcc acatttggtt gattgtaatc tgtaaatttc
     6241 acgtattcca aattaatgtt ctcctttttc aaacggtctt tcacgttgtc ccatacatct
     6301 gtatcttctc caataatccc tagttttacg atagtttctt ttttgtcagt tgtatctgct
     6361 tttccttggc tacaccctgc caaccaaata attgctacaa atgtaatgac tacaaaacga
     6421 ataatttttt tcattttttc tcctccactt tcaatgtgta atttttttaa tcagttgatt
     6481 actgataaat tgacttaaaa aaactaagat tagaatcaat aatgtagcca caatcgttac
     6541 atctgtttgg aatcgattat accctctcga tatagcaaga ttccctaatc cgcctgcacc
     6601 tactgctcca gccatggtag tcagtccgat cacgttgatg atagtcaacg ccgagacccg
     6661 aatgatacca ggcaaacctt ctaacaaata cacacgtaaa atgataccga ttggactggt
     6721 tcccatcgct tctgccgctt cgatcacccc aggatcaact tctagcagtg cgttctcgat
     6781 ttgacgagca aaaaacggaa caactccaaa aatcaatggc actaaagctg ctttcgcacc
     6841 aatcgtcgtt ccaactaaga aacgagtagt caatgctaaa agagaaagca agataatgaa
     6901 agggatggaa cgaatgatat tgatcagctt atcaagtatt tcaaatagca gacggttagc
     6961 tagtaatcca ttagggcgtg tcactactaa cgtcacacct aagatgattc caatcatccc
     7021 gccaatgaat gctgtccaaa aagacatata tagtgtttgc ttcgtcgctt cgacaaattc
     7081 atccggaatc aaataaacat tgggcaaata tgttttaaag aaatcagtca ttttatcttc
     7141 ctccttcgat cactttgata cgactatcta aatcgttttt ttgtaaaata tttattttta
     7201 caccagattt gatcaaataa tccaatgctt ttttccgctg tttggtttct ccagctaaag
     7261 taacgatcaa actccctaac ggcgtttctt gtaataactc tacattgcca tataaaatat
     7321 tcgctgttac ttgatatttg ctgtacagat gagcgattag aggttcgttc gtctgatttc
     7381 caatataact taattccacc agccatttat tggcgatctg atcggcaaaa gcagaatggg
     7441 caatgatcgt ttctaatgct tggtcaagat gagtagctgt tcgaataaaa tctttggtca
     7501 attcttcttc aggattgctg aagatctgga tgctgctacc tttttcaacg attcttccat
     7561 cttccataac cgctaccttg ttgcagattt ctttgacaac ttgcatctca tgagtgatca
     7621 ggacgattgt taaacctaac tggcgattca atttttttaa caatgcaagg atctgaaggg
     7681 tcgttttcgg atcaagtgca ctggttgctt catcacataa taatacctta ggttcattcg
     7741 ccaaagctcg tgcgattgct actcgttgtt tttgtccccc cgacagttgt ttaggataag
     7801 cagatgcttt ttcttccaaa ccgacaagtt ccagtagttc attcactttt tgacgacgtt
     7861 cctgtttcga ttttccagaa tactttaaag aaaaatcaac gttgtcaaaa atagttcggc
     7921 tttccattag attgaaatgc tgaaagatca taccgatggc ctttctttct tttcgcaatt
     7981 ctttgggaga gagctgactc agattcgtct gattgacaat gacttctcct gctgttggtt
     8041 tctgcagcaa attcatcaca cgaactaacg tacttttccc tgcacctgag taaccgacga
     8101 ttccataaac atcgcctttt tcaatcgtta aatctacaga atctactgcc tgtatctctt
     8161 tttctttttg tttgaaacat acagaaacat ctcgcaattc aatcattaaa attcctcctt
     8221 atctaaaaaa agcgtcctca agaaaatgaa aatgtcattt tcttgaggac gcttttttgc
     8281 gtgttaccac ctcatgttca agctgtgttc acacacaact cctcttcaag tacgatcata
     8341 ctctagcaag atatcggtcg cagccgtcat cgccttacat ctatgctcgg gatgaattgc
     8401 tcgatggcca ttttccaata gtcgttcctt gcttcttctc agctaccgaa gctctctgtc
     8461 aaaggttctt ctatcgtact taccatttca atgcaaataa tattcaaaaa aaaagtcctt
     8521 ttactaacat aaactatgtc agtaaaagga cgacgtatcg cggtaccacc ttttttcgga
     8581 agtttccttc cctcaagccc aacatctcta aaagagagat tggcgttcgt tttaacggtc
     8641 gaacagttcc gaatttgcct acttatcgac aaattattct ccaaggccat tttcagcttt
     8701 tcttacttga ctcttctcac cagccagagc ctctctgcaa agtttccaaa cttactttcc
     8761 ttttcaacga aatcgatatt ttgttgttgg gattaatcta catgaggaaa actgaaaagt
     8821 caagtacgaa attcaaatta ttttttctat atttattttt ctgttttttt atttaacatt
     8881 tcaataacag ttcgaagatc ttttccttcc gcaagaagtt tcaatttttc cttttcttcg
     8941 acggtataat ttcggacaaa actggaatca ggtatcaaaa caacgcctaa tcctgctcgt
     9001 gaagcagctg ttgctcctac taaagaatct tccactgcaa aggctgaact agcttctaat
     9061 cctgttcgtt ccaaagctgt cagataagga atcggtgaag gcttgttctc cgacacttcg
     9121 tctccgaatg taaacgtgtc caagtaatca gctaatttaa agtgatccaa gatatctgtt
     9181 gctcttttcg taacagtggt cgtagcaatg cctagtttca tatgacgttc ttttaagtct
     9241 gccaatattt cttttgcata tggttttaag tggatggctc cgcttgtcag ctgttgataa
     9301 aaatattctt cacgcttcgc tcggacttcc tgtacttttt cagggctacc tgctgctttc
     9361 actaaaatat caaaactttg tttccagttt tttcctcccc aagcgtcaac gatttctttt
     9421 gggatatgga tatcaaattg ctgaaatccc caaagccagc catcacgata cgttttttct
     9481 gtgtcaacta acaaaccatc taaatcaaat attgcacctt tcatctatac attaatcctt
     9541 tccaaaacaa tacacatagt ttagcacgac tggtccaaaa agaacagaag caaagaacca
     9601 gccttctcgt ttataagagt tcttggaatc gacggatata gattttcttc acagctgtca
     9661 caagcgccag ataagcgatg actgtagcaa ataaccataa ccagtaattg ccaggaagtg
     9721 gcatcaagcc taattcagca ccaaaacttg taaaaggaag gacagaaccg acaataattc
     9781 ctaatgtagt gataacaaac atggcaaatg ttgcgttgct ttggataaat ggtactttcg
     9841 gtgtacgcag tgcatggagc actaaggttt gtgtccatag tgattcgaca aaccatcctg
     9901 catggaataa tgcgatgaaa atcactcgct gtgaactatc caatgtatgg aaacttccgc
     9961 ccactacagc aggacaaata acaaaataca tcaacaagta agtagtaata tcaaatatcg
    10021 aacttgttgg accaaagtaa accatgaatt ccccaatact agatgcattc catttttttg
    10081 gctcgtccaa atattcttta tccatccgat cccatggcaa ggaaatgcag gaaacatcat
    10141 agatcagatt caaaaacaaa agttgtaaag gcagcattgg taaaaatggc aggaacgtac
    10201 ttgcaatcag cacggaaaac atattgccaa aattagaact tgctgtagcc ttgatatatt
    10261 tcatgatatt cccaaaagtt tctcgtccag aaagaaggcc tctttccaaa atcgtcaaat
    10321 ctttttctaa aaggatcacg tctgcagatt ctttcgcaat atccacagct gtatctacag
    10381 aaataccgat atcagccact ttcattgcag gagcgtcatt gataccatct cctaaaaatc
    10441 cgacagtatg accattttta cgtaaagctg tcgtcaaacg tgctttttgc tgaggattca
    10501 attttacgaa tacttcatgg tcttcagcta cttgtgctaa ttcatgatca ctcatttccg
    10561 agattttctc accggtaacc aattcattga tctctagacc tacttgacgg cacactgaac
    10621 gtgtaactaa ttcgttgtcc ccagtcagaa cttttacagc tacaccatgt tctttcagag
    10681 ctttcaacgc ttgtttcgtt gtttcttttg gtggatcaag gaaagcaaga taaccaatca
    10741 aaaccatgtc tgactcatct ttgactgaaa attctccaac gacgcttgga ttggtctttt
    10801 gtgcgacacc aatcactcgt aagccatctt cattaagtga ctgtactttt gcaagtacgg
    10861 cttcttttcg ttcttttgtt aatggtgtaa cagtaccgcc aagatcaata aatttagaaa
    10921 tgttcagcat ttcttcaatc gcgccttttg tgatcatttg tgttttacca gctggatcag
    10981 caaccacaac actcatacga cgacgttcaa aatcaaaagg aatttcatcc actttttgat
    11041 aattgatctt atctgtatct aaagtttctg ttgcttcatc aatgatagcc acatctaaca
    11101 aatttttcag ccctgtttga tagtagctat ttagatacgc atggcgcaaa acacgatcat
    11161 cttcatttcc ttcacaatcc atatgatatt caagaatgat tttatcttgt gtcaatgtcc
    11221 ccgttttatc cgtacataac acatcgattg caccaaagtt ctggatggca ttcaaatttt
    11281 tgatcactgt tccttttttg gccatcacac ttgctccttt gaccaaattc gtcgtaacga
    11341 tcattggaag catttcaggt gttagaccaa ctgctacaga tagtccaaaa agaaaagctt
    11401 ctagccaatc cccttttgta agcccattga tgacgataac tgctggagcc atcaatgaca
    11461 taaatttgat cagtagcatg gatgttttat ggatgcctaa ttcaaagctt gattgaattg
    11521 gtttttcaga tagcgtttct gctacatgac caaatagtgt ttcatttcca acagaaacaa
    11581 tgattccttc cgcacttcca ctgatgacat tacttcccat aaagacaaga ttttcgtaac
    11641 ttgtttcgct ttgctgatcg tcttcacata ctttcgcatg cttttctact ggatagcttt
    11701 cccctgtcaa tgctgcttgg gaaatgaata aatcctttgc ggacaataaa cgcatatccg
    11761 ctggtatcat atcccctgct gaaagccgaa caagatctcc acagacgatt tcttccatag
    11821 gaatctctgt gtactttcca ttacgtttta cagctgccgt tactttcaca agtgatttca
    11881 gtttttcagc cgcttgattg gaacgtacgg actgaatcaa cgtcattgtc ccgctgacga
    11941 taaccatagt gaagataatg gctacaccga aaagatcttt atctccagga gctgctaata
    12001 catattccgt gatgaatgaa ataattccta atgcgatcaa cactaatgta aatggcgtga
    12061 tgtatgcttt caatatttct acaatgaatg gtgttttttt gccatatgaa atcgcatttt
    12121 caccgtaatt ttctcttgaa cgttcgattt gttcttctgt caatccattt gctgatgcat
    12181 ggaatagttc aaataattct tgtttcgttt ttccagcaaa agctgcatag attttttgtg
    12241 tatctttctt tttttccatc tcaatctctc ctttgtgtcc aaaagacagc tgttctcgct
    12301 gcttttggtc tttagctatt catcggatcg atagagaaaa aactgaaagg ggctgagaac
    12361 agatagataa tagccctggc ctttcctcta gatccttgtt tttcgaatga ctgtcatcca
    12421 tttgttctca tctcctttac taagtaattt taattcatga cttccaatca tttttcgaat
    12481 agttattttt ccaattagac cacctccttt ttatttttca ccgatgggga tttgcggcaa
    12541 caaaaaaacc tctcgccaaa tactcagcga aaggtttgaa aaagcatatt aaaaaaagct
    12601 tctcagatct catcgttgag ttttgacact gtacaacgta aaaagcagat acctgctttt
    12661 agctacctta tgaaaagcct tatctcgaca attcctgttc tacccattgg cgtctctcga
    12721 cgtttttggg cagtagccta tgtttgtata ggagcctcac ctaacaaatc ttattcggtt
    12781 ttccttcgtt aatataacaa ctaaaaaaga ggatgtcaaa tcaattttcc atgtttttca
    12841 tacttttttt aaaatacaca cgttatattc ctgtctcacg cggttttatt tgtctcattt
    12901 tccacctccc gctataatag aaatatagta ggaaacgagg tgctgttatg gaaagaacac
    12961 ttgttattat caagccagac ggcgtgagac gccatttagt cggaagcatc atccaacgtt
    13021 ttgaagcaaa gggactggcc attgctgaaa tgaagtttga aactatgact cctgaattag
    13081 caaaagaaca ttatcagcac ttgactgagc gcagtttttt tgatgaactg atcgattaca
    13141 tgacttccgg tcctgtcgtt tatcttgtgc tggtcggcga ggaagtgatc gatatcgtcc
    13201 gtaagatggt tggagcaaca aaagctgccg atgctgttcc aggtacgatc cgaggagact
    13261 acgcgctgcc ggggactgaa aacatcattc acgcttctga ttcccgtgat gctgcggtaa
    13321 aagagatcgc ccgctttttc ccgccattcg atataaacaa accgtctgaa aaagcggcga
    13381 agtgatacaa attctaaact gctggtctag ttgctgggtt cagcaatggc cagcagtttt
    13441 ttctctaagg acctccgttg attctttcct aacatatatt cgagcactgt gaagaattct
    13501 tggtagtctt tcgtcttttc ttcccctaaa gcagcagaaa tcaatctttg ataataatct
    13561 gccatcaatg tttcatagat cactgtactt ctttctttat gtgctaagct ccgtttcata
    13621 tcttcattca cactacgtgc ctttttcaac cgaccatttt ctatataaaa cgaagtcagg
    13681 ttcattaaaa aagtaaagag atactgctca tgattgttca actgtgctaa tcgtcgcatt
    13741 ctttttttga ttttcaaata attgttctca atgaaccgat cactgaatat ccaaggaaaa
    13801 tcagctagaa tagatagttc aaattgacca tagtctggta tctgcatcaa gtattcttca
    13861 atgattcggc attcataacg taaaaattca aattctgtct cagatacttc tgttttttcg
    13921 accatgtgaa gattcaaaac caaataatac agatcttctg ttttcttaaa atgcctacgg
    13981 ttattgctga tctctttata aggatcaaag ctgtcttctt gtacccgatc tagcattcgt
    14041 ctttttttct ttaactttat tggttcatcc agctggtttc gcatccattc atattctgca
    14101 agtgtcatgt tcattcgctc taaaaatgat ctcaatgtta caaatgacat ttttttcact
    14161 gaatttgtct caatcccgac tagagaagtc ttaaaacaga ttccttccgc taattgggac
    14221 tgtgtcaact tcttgttgac tcgaagtttc ttgatcaatt caccatcata cataatagtt
    14281 ctccttttta atttaaattt ttccacattc aataaataga tacgttaaaa aggaaaaata
    14341 cacgaaaaaa ataaaaaaat gtttttttca taaaaaatac caaccttcaa gcattagaat
    14401 gt
//
LOCUS       DAIGOK010000069        14171 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.069, whole genome shotgun sequence.
ACCESSION   DAIGOK010000069 DAIGOK010000000
VERSION     DAIGOK010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.069"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..134
                     /locus_tag="NOA01_001685"
     CDS             <1..134
                     /locus_tag="NOA01_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCI0632877.1"
                     /translation="VNQTFISSVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
     gene            complement(145..555)
                     /locus_tag="NOA01_001686"
                     /pseudo
     CDS             complement(145..555)
                     /locus_tag="NOA01_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(658..1953)
                     /locus_tag="NOA01_001687"
     CDS             complement(658..1953)
                     /locus_tag="NOA01_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCI0632878.1"
                     /translation="MNDSIKKMLRLIDKNLMITGVSYETFQKEKTLIVDAILSPAPCA
                     CSNCGSTVVDGTGKTIVVKNGKKETIVRFEQYNHMPMVMRLKKQRYTCKNCRTHWTAQ
                     SYFVRPRHSIANHVRYKITSLLTEKVSLSFIAKSCQVSLTTVIRILKEFKSYLPKSSK
                     RILPKVLMVDEFRSHASIEDKMSFICADGETGKLIDILPTRKLPRLTSYFLSCTNPEE
                     VEFLITDMNAAYFQLTKRVLPNAKVVIDRFHIVKHINQAFNDLRIREMNELRKAGKKR
                     QAEKLKKNWRFLLKKRSNINHYEYKTWKSFRAPKYPFFTEAMMIDRLLGFSTPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLEELPETLDDAFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLIKVK"
     gene            2134..2444
                     /locus_tag="NOA01_001688"
                     /pseudo
     CDS             2134..2444
                     /locus_tag="NOA01_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009559264.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
     gene            2952..4244
                     /locus_tag="NOA01_001689"
     CDS             2952..4244
                     /locus_tag="NOA01_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family permease"
                     /protein_id="HCI0632879.1"
                     /translation="MDGITIPWYAAIIGLIFAVFLILKKMNPVYSLFLGAIIGCLIGG
                     ASLSNTVDILVTGTQSIMGTAIRVLAAGMLAGVMMESGAAESIAHAIVNKLGSNKAIF
                     SLALSTMIITAVGVFIPVAVLIVAPIALSVGKQTGISKLALLTALSGGGKAGNIISPN
                     PNTIAAANGFKLDLSNVMIAGFIPAIFGLATAVLIASLIKKKGGKVKDSDIQEKSEKE
                     LLPLRKALIAPVLSIVLLLINPIGTMLHIQVLADLKIDAMYILPFASIVGTIAMGQAS
                     QIREYSKSGIYRVTDTVLILIGAGAIAGLISVSDLSKQVVHLISITGISGTLLAPISG
                     ILMGLAVGSTSTAVILATGSFGHAILETGTNSLAAAVMVHTGATVIDSVPQGNYFHIT
                     ANSMNMSIKERMKVIPYEICVGGVMTIVATILYGFILK"
     gene            4379..5524
                     /locus_tag="NOA01_001690"
     CDS             4379..5524
                     /locus_tag="NOA01_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCI0632880.1"
                     /translation="MEKTFVLAPDSFKESMSAKNACEAMERGIRKVFPESKVIQVPMA
                     DGGEGTVDALVDGINGTKIEVMVSGPIPSEKIKAYYGLLADESTAVIEMAEANGIELL
                     AENKRNPLLTSTYGTGEMIKDALDKGVKKIIIGIGGSVTNDGGAGMAQALGVQFLDKN
                     DKELPVGGGALSELAKVDVSTIDPRLKETEVVIASDVTNPLTGPNGASFVFGPQKGAT
                     PDMVKKLDENLVHYAAVIQKNLGIDIKDQPGSGAAGGLGAGLLVFTGAKMESGVDLVI
                     QLTHLEEKIVQADYVFTGEGGMDFQTKFGKAPYGVAKIAKRYDKPVFACAGYIGEQVD
                     VLYEEGMTAIFGILDQAGPLKQALQAGESNLERTVENIVRVLNVCGG"
     gene            5594..6607
                     /locus_tag="NOA01_001691"
     CDS             5594..6607
                     /locus_tag="NOA01_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCI0632881.1"
                     /translation="MSNLLTKEQANMIVSKLMADIPYNINIMNEQGFIIASGKEYRIG
                     ERHRGAERAIKQGEMVEIFKDTSLEKRGTNEPIILNGELLGVVGISGDPEEVRPFTKL
                     VKSIVLLLVEELNEFRKKEKKNQLKADFLKELLSIKDCYSEEMVHQALDDYGINLLRT
                     NRCIGSLEQETLRSCFPLHEVFEWYGMYIVFIDGNTIIDDDLSGLIVSSEQWNLGQGM
                     NEVDNTFLYLKFFQIPINKVFFSEDLYFIHLFDFSIKLDKNLLKKVESIYDEYYETLI
                     YFSSNNGNVKEVSEKLHIHRNTLNYRIQRIQELTRKDPKNWSHLWILMYHLAYCFKVK
                     FRT"
     gene            6999..8198
                     /locus_tag="NOA01_001692"
     CDS             6999..8198
                     /locus_tag="NOA01_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCI0632882.1"
                     /translation="MRDQTSGKMIRFYYTSLQFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFISQVIIKKRKIPLITVLKTMTAGLGVILLAGVLLPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVVFFVILHLLPDVKNQEVFPMTKEQDRPVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSIASISFAVRGFMMLLAGSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTSFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
     gene            complement(8693..9451)
                     /locus_tag="NOA01_001693"
     CDS             complement(8693..9451)
                     /locus_tag="NOA01_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCI0632883.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLF
                     KDDIVIFVSLNGETKELITAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
     gene            9619..10554
                     /gene="pfkB"
                     /locus_tag="NOA01_001694"
     CDS             9619..10554
                     /gene="pfkB"
                     /locus_tag="NOA01_001694"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCI0632884.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            10551..11996
                     /locus_tag="NOA01_001695"
     CDS             10551..11996
                     /locus_tag="NOA01_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCI0632885.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSIIAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAVLFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAATVEMKEETNN"
     gene            12024..12479
                     /locus_tag="NOA01_001696"
     CDS             12024..12479
                     /locus_tag="NOA01_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCI0632886.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            12498..13472
                     /gene="lacD"
                     /locus_tag="NOA01_001697"
     CDS             12498..13472
                     /gene="lacD"
                     /locus_tag="NOA01_001697"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCI0632887.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNQSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(13582..14067)
                     /locus_tag="NOA01_001698"
                     /pseudo
     CDS             complement(13582..14067)
                     /locus_tag="NOA01_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
BASE COUNT         4434 a         2288 c         2987 g         4462 t
ORIGIN      
        1 aagtgaatca gacatttatt tccagtgtca gcaatcaacg taatcatatt ccaagaaaat
       61 cattgaatta cagaacacca atcgaaatat ttttgagcta tgtacaagaa gcattttatt
      121 ctagcttaat ttgacaaatc atattatatt ggacatggtt tggggcgtgt agtgatgccc
      181 atacattttc tcaatcaagt ctgcgatttc cgacatggta ataccttttc ggaagaggtg
      241 aatgacggtc tcatctaacg tgtcattcgt ccgtctataa gcaggaacag tctgttgctt
      301 gaactcgcca ttgcggtcgc gcggaatctg gagatgaagt tccccatact cggtcttgac
      361 cgtacggtca taggagccgt tacgagaatt acccgtgtta aaaccaatgc gatcatactt
      421 ttcgtaatcg aggaaagccg ttaactccgt tttgagaagc gtattgactg ctttctccaa
      481 atggacacgg aataattcat tcaaatcgcc tttgttcgct agagtcttta gaatttctgt
      541 agtaaaatcg ttcataggga agtcctctct tctgggctct ttgtcaataa ggactgatga
      601 agctgtgcaa aatcaaatct gggtcaggat gaacctcatt ctggctcaga ttttttgtta
      661 tttcactttg attaattgat tgatcaaaaa gattctaatt ctcatgttct caaatgattt
      721 aaatccgtag gatactcgtt tcattgtctt tatatgggta ttctttgctt ctatttttcc
      781 attagaataa ggatagatca tcgcattggt gatgccttct tcataggtca gaagattttg
      841 aagtttctca cgaaaggcat catctaacgt ttcaggaagt tcttccaata aagagaaaaa
      901 taagtcaggg tctttgtctc gaaaggcttc tactaactca tgaaaaaagg gatacgcctc
      961 cttcaggggc gtggagaaac caagcaatcg gtcaatcatc atcgcttcag taaaaaatgg
     1021 gtatttaggc gctcggaaac ttttccatgt tttgtattca taatggttaa tgtttgaacg
     1081 tttttttaac agaaagcgcc agttcttttt caacttttca gcctgccttt ttttacctgc
     1141 cttacgcagc tcattcattt cacggatacg taaatcgttg aatgcctgat ttatatgttt
     1201 gacaatatga aatcgatcaa tcactacttt cgcatttgga agaacacgtt tggtgagctg
     1261 gaaataggcg gcgttcatat ctgtgattaa aaattctact tcttctggat tggtgcaact
     1321 taagaaatag cttgttaatc gaggtaattt acgcgtaggc aagatatcaa ttaatttacc
     1381 tgtttcgcca tctgcgcaaa taaagctcat tttatcttct atagaagcgt gcgaacgaaa
     1441 ctcatcaacc atcaatactt ttgggagaat cctcttagag gactttggta aatagctttt
     1501 aaactctttc aatatacgaa taacggtggt caaagatacc tgacaacttt tcgcaataaa
     1561 agataaagac actttttcag tcagtaaaga agtaatttta tatctgacat gattcgcaat
     1621 ggaatgtctg gggcggacaa aataactttg agcagtccaa tgggttcgac agtttttaca
     1681 ggtatagcgc tgctttttta agcgcataac cattggcata tgattgtatt gttcaaagcg
     1741 gacaatggtt tccttcttgc cgtttttcac cacaattgtt ttcccagttc catctaccac
     1801 agtagaacca caattgctac aggcacaagg agcaggtgag agaatggcat cgacaatcag
     1861 agtcttttcc ttctgaaagg tctcataaga gacccctgta atcatcaaat ttttatctat
     1921 taatctcagc atttttttga tagaatcatt catatagcat atcgtcctct cagtggttta
     1981 ttttgtcgtg atttaatcat actagagaac gatatgtttt ttaatatcta aaatgaaaat
     2041 gagactgaag aatcaattct gattcatcag tctcataaat tatagagcca caaaactctg
     2101 tggtcttttt ttgtagtaaa ggaggaattt ggagtggcca aactagatga agaatttgct
     2161 tttttggtat tgtcgatcat atcggagatt cccaaaggca aagtagctac ctatggacaa
     2221 attgccgagt tggcaggata tcctcgcaat tcccgtttag tcggtaaagt attgagccac
     2281 gccgaatatt atggtcgcta tccttgtcaa cgagtagtga atagtcaggg agaatgtgcg
     2341 aaaggttggc cggaacagcc gttactattg caagagaggg tattattttt cgcgagaatg
     2401 gacgagtgga tctgaaacgc tgccattggg atggagaaga gtgatctgta aaggatcatt
     2461 cactttaagc aatacagata tgatgaaata aaatatctat gaattttttt gcgttgcaat
     2521 ggagagattc tttgggagtt tgattctaaa ataaatcata tacaatgggt tatttagtgc
     2581 ccgattttgt taggagtata ggcaaaaaga attagtagtg aaaatcagta ttgtatttgg
     2641 gatatattct tttatttaat atgagttagg tccttagaac taaaagaatt aagagattga
     2701 ttgactttgt aaatagtaaa atacctaata agtatcaatg agttcccaaa tcgtatctag
     2761 ttgaatagaa gaaatttcta ttgaatacta tgtgattcta tgaatttatt taagttgtct
     2821 aaaatagtga aattctgaat ttatattatc gatatgtgca aatgaacaaa taacattgta
     2881 aatataccaa ttttatttat gaaataaagc attaaaatga aaacgtaaac aattaagaaa
     2941 ggatgtttga aatggatgga attacaatac cgtggtatgc ggcgattatt ggtttgattt
     3001 ttgcagtttt tttgattttg aaaaaaatga atccggttta ttcattattt ttaggagcaa
     3061 ttatcggatg tttgatcggc ggagcatcac taagcaatac cgtggatata ttagttacag
     3121 gaacacaaag tattatgggg acagcaattc gtgtgttggc agcagggatg ttggcaggag
     3181 tgatgatgga gtcaggcgca gcagaatcca tcgcccatgc aatcgttaat aaacttggaa
     3241 gtaataaagc aatcttttca ctagctctgt caacaatgat cattacagct gtgggcgtat
     3301 ttattcctgt cgcagtattg atcgtagcgc cgatcgcttt gtcagtcgga aagcagacag
     3361 gtatcagtaa attagcattg ttgacagcat tgtctggtgg cggcaaagct ggaaatatca
     3421 tttcgcctaa tcccaatact atcgcagctg ctaatgggtt taaattagat ttaagcaatg
     3481 taatgattgc tggatttatt ccggcaattt ttggattagc aacagcagtt cttattgcta
     3541 gtttgattaa gaaaaaaggc ggtaaggtta aagatagcga tattcaagaa aaatcggaaa
     3601 aagaattgct tccattacgc aaagctttaa ttgcacccgt tttgtcaatc gttttattat
     3661 tgataaatcc aattggaacg atgcttcata ttcaggtgtt ggctgatttg aagatcgatg
     3721 cgatgtacat tttacctttc gcttcgatag tggggacgat cgcaatggga caagctagtc
     3781 aaatcagaga atattcaaaa tcggggattt atcgtgtgac agataccgtt ttgatcttga
     3841 ttggtgctgg tgctatagct ggtctgatct cagtttctga tttatcaaaa caagttgtcc
     3901 atttgatttc cattacaggg atttcgggta cattattagc gcccatctca ggtattttaa
     3961 tgggcttagc agtaggatca acttctacag cagtaatttt agcgactgga tcttttgggc
     4021 atgctattct tgaaaccgga accaattcgt tagcagcagc tgtaatggtt catacaggtg
     4081 caacggtgat tgatagtgta cctcaaggaa actatttcca cattacggct aacagtatga
     4141 atatgtctat caaagaacgg atgaaagtga ttccttacga aatctgtgtc ggtggtgtga
     4201 tgacgatcgt tgcaacgata ttatatggat ttattttaaa ataaaattgt agagatagcg
     4261 ttttgtcaaa tcttttacaa aaatatgaac ttgaaaaaat atttattgaa aataacaatg
     4321 aagaaggtaa attctgtttg aaaagaagat tttcagcaga attttgagga gaagaacaat
     4381 ggagaaaact tttgtattag caccagattc atttaaagaa agtatgagtg ctaaaaatgc
     4441 ttgtgaagca atggaacgag gaattcgcaa agtatttcct gaatctaaag tgatccaagt
     4501 ccctatggca gatggaggag aaggaactgt tgacgcacta gttgacggta ttaacgggac
     4561 aaaaatcgaa gtgatggtgt ctggaccaat tccatcagaa aaaatcaagg cttattacgg
     4621 gttgttagca gatgaaagta cggcagtgat tgaaatggct gaagccaatg gtattgaact
     4681 attggcagaa aacaagcgaa accctctttt gacctctact tacgggactg gagagatgat
     4741 caaagatgcc ttggataaag gagtaaaaaa aataatcatc ggtataggag gcagcgtcac
     4801 gaatgatggc ggtgcaggta tggctcaagc gttaggagtg caatttttag acaaaaatga
     4861 caaggaacta ccggttggag gcggcgcgtt gtcagaatta gctaaagtcg acgttagcac
     4921 tatcgatcca agactaaaag aaacagaagt cgttatcgct agcgatgtaa ccaatccgtt
     4981 gacgggtcca aatggcgcat catttgtttt tggtccgcaa aaaggagcta cgcctgatat
     5041 ggttaagaaa ttagatgaaa atttggttca ctacgctgcg gttattcaaa aaaatttggg
     5101 gatagatatc aaggatcagc ccggttctgg tgcagcaggg ggattaggag ccggactatt
     5161 agtttttact ggcgcaaaaa tggaatccgg tgtcgatttg gttatccagt taacacattt
     5221 ggaagaaaag attgtacaag ctgattacgt gtttaccggt gaaggcggta tggattttca
     5281 gacaaaattt gggaaagctc catacggtgt agcaaagatc gccaaacgat atgacaagcc
     5341 tgtttttgct tgtgccggtt atatcggcga acaggttgat gttttatacg aggaaggaat
     5401 gactgcgatt ttcggcatct tagaccaggc cggtccattg aagcaagcat tacaagccgg
     5461 cgaatccaat ttagagcgta cagttgaaaa tatcgtccgt gttttaaatg tttgtggagg
     5521 ttaaaagttg tgttatctat caaaaaaaga ttaaactaac tgtataacca gataattctt
     5581 aggacggtaa tcaatgagta atttattaac aaaagagcag gccaatatga tcgtaagcaa
     5641 gctgatggcg gatattcctt acaatattaa tattatgaat gaacaaggtt ttattattgc
     5701 aagcggtaaa gaatatcgta tcggtgaacg acatcgtggt gcagaaagag caattaaaca
     5761 aggcgaaatg gttgagatct tcaaggatac ttccctagaa aaaagaggaa ccaacgaacc
     5821 aatcatttta aatggcgaat tattgggagt agttggaatc tcaggagacc cagaagaagt
     5881 ccgcccgttt actaagctag taaaatcaat cgtactgctt ttagttgaag aactaaatga
     5941 atttcgaaaa aaggaaaaga aaaatcagct taaagctgat tttttaaagg agctcctttc
     6001 aatcaaagat tgctacagtg aagagatggt tcaccaggca ttagatgatt acgggataaa
     6061 tcttctgcga acgaatcgct gtattggatc attggaacaa gaaactttac gtagttgctt
     6121 tcccttacat gaggtttttg aatggtatgg aatgtatata gtttttattg atggcaatac
     6181 cataattgat gacgacctct caggtttaat cgttagctct gaacaatgga atttaggaca
     6241 gggaatgaat gaagtagata atacttttct gtatttgaaa tttttccaga ttccaatcaa
     6301 taaagtcttt tttagtgaag atttatattt tattcactta tttgattttt caattaaact
     6361 agataagaat ctattaaaaa aagttgaatc aatctatgat gaatattatg aaacactgat
     6421 ttatttttct agtaataatg gaaatgtgaa agaagtgtca gaaaaactgc atatccatcg
     6481 taatacacta aattatcgaa ttcagcgtat tcaagaatta actagaaaag atcctaaaaa
     6541 ttggtctcat ttgtggatat taatgtacca tcttgcttat tgtttcaaag tgaagttcag
     6601 aacgtaagaa ataagaatat gtatccataa taaatgtttg gatttacctt atagatataa
     6661 aaagaaacaa atcattctgc aggaatcgat tactcgattc ctgctttttt tacttccttt
     6721 gcagcaaaaa agtgctcgta atctaagcga tgttggatag tttttagaat atatatattt
     6781 tcttcaattc cagaaaaaaa tgaccaagct agtatgctaa aaaagtacga tgcttctatg
     6841 atcttttttc cgtctttatt aacagatgtt ttaattgatc aacgcatatt gtaatctacc
     6901 ctaatttcca tagttcttca ccacttttaa aaatagtaaa acccctacaa gtatgctata
     6961 gtaaattgct aaagaaaaac caataaggtg ggagatgtat gcgcgatcaa acaagtggaa
     7021 aaatgattcg tttttattat acaagtttgc aatttctcta ttggatgatg ttttgttcgg
     7081 tctatgggtt tgcgacattg tttttgattt atttcaagat tgatccagct aaaatcggag
     7141 ggatcttagc attggtcaat attgtttcaa cggtgcttca gccttttatc agtcaggtga
     7201 tcatcaagaa aagaaagatt ccactgatca ccgtgctaaa gacgatgaca gcaggattag
     7261 gagtgattct cttagctggg gtattgcttc cgaatttgcc gatcattact tatatcttgg
     7321 gtgggatttt agtagtttcc atgcaatcat ttctcaatgc gctggcattt gagtattcaa
     7381 ataccggcta caggatcaac tttggttttt caagagctgg cggttcattt tcttatgctg
     7441 taacttcttt tttactggga cagttgttag cgatttattc tgccgcgatt ttgccgatct
     7501 tggttatagt tgaagcagta gtgtttttcg ttattttgca tctgctgccg gatgtaaaaa
     7561 atcaagaagt ttttcctatg acaaaagaac aggatcgccc agtttctttg agaaaaaaat
     7621 atcctttcct gattttgttg tttttaggtt tcagttttct ttttactttc cacaccatga
     7681 tcaacagttt tttggcacag atctttgaaa acatccacgg tggcagtaaa gaagtcggca
     7741 tcgcattgat gatcgccgct ttatgcgaat taccgggaat gatctttttt gaacagcttg
     7801 tcaaaaagaa atccagcaaa ttttggctgt ctattgccag tatttcattt gctgtcagag
     7861 gattcatgat gctgctggcc ggttcgatct tgatgatgga aatgacgcat ctcctgcaag
     7921 cctttagttt tgctttgttt attccagctt cagcttattt attcaatcaa tttctggcgg
     7981 atgacgaccg tgtatttgga caaacgatga tcattatcgc cacaactctt ggtggggtga
     8041 taggaaatgt cttgggcggc agtctgctgc agcatttcgg tgtatgggag atgctgattt
     8101 tcggtactag ttttgcggtg ctaggtgctt tattaactat tttgggcatt cgaaaaatac
     8161 cgaagacagg aaacgatagt gattctcttg ttgagtagca taaaaatact tagatactag
     8221 ttttatataa ataacatata gtgattattg gtgtaattac aaaaaacgag aataacttaa
     8281 gtaagcattt ctaaataatt gagaaacctc atctaaaaag ataagaaaat atccctaata
     8341 aaacttctgt aacattttag ataactgatt gtaagctgaa taataaacta taaggagaca
     8401 gttagacggt ctcctttttt tgcgtattat aattctagaa ttgttggaaa ttgataataa
     8461 ttaacaagaa atattagaca ggaacttcga actgcttatc acattcttat tattatatat
     8521 ttgcgtaagt agtcctggta gtctcaatgc actattctat cttgaaacta agaagttata
     8581 gaaggagcaa tgatagaaag cacgagaata caaatagcga ttaaatattt ttctagacaa
     8641 ccttagttaa ttcacacaga tatattttag gtacagtctt acagatatag agttagtgct
     8701 gtgtgaatcc tgatgttcgc agtgcatagg agtcaagtaa gattctagcc agtatagaaa
     8761 gtggaagtct ggagcgtacc tcataatctg ggaagaagga gccttctgat ttaaagccaa
     8821 caaaaggaat ctcgatatgt tccatcaaac ttgaatgacg attagcagta agcaaaattg
     8881 tcccgatctc agctttataa cagttttcag ctgcggtaat caattccttt gtttcaccgt
     8941 ttagtgaaac aaagatcacg atgtcatctt tgaataattt tctactaatg ttttttatga
     9001 tattgggatc agtatgcatc tcacaatatt taccggtaag ttgaaatttg accatcattt
     9061 cctgagcaat caattctgaa aaacctcggg caaaaatcat gattcttcta gcagccttga
     9121 ttcgttgaat agcatcttca atattcccgc tttctaccat attgatggtg cggattacct
     9181 cttgctcatt tttaagaata gcagtcttta ttccttcatc aactttatca agagcggcaa
     9241 aattgatatt tgtattttct ttttctttta acgaatgttt aaaagacgta aacccctcat
     9301 atcctttttt cttcatagta cgaacgacgg ttgctgttga aacgttggca tattcactta
     9361 gttttacaat ggaataggag gggatttcga gtatgtgact ttgaataaaa tcccagagat
     9421 atttctccga ttcacttaat tttgtatcca tattttgcct ccgattagtt aggataattc
     9481 tattgtaaag cgggagtttc ctttacttca agtttattta atatattttg taaaagtttt
     9541 caatgaattt agtcatttat gaaaacattt acattcgatc ttgatacata atgaatatgt
     9601 aagaaaggag gagcgtaaat gtcagtttat acatgtactt taaacttggc aatcgacctt
     9661 tttatcgaaa cagatgaaat gaaaccatcc caagtcaatc gaacaaacga tgatgatata
     9721 caagctaatg gaaaaggggt gaatgtttca ttgatcatga agcaattagg tatcgatagt
     9781 acagctttag gatttaaagc aggttttacc gggaattata ttgaggatta tttgcatcaa
     9841 aaagatatta gcactgattt tattagcgta ccgggaatga ctagaatcaa cgtttttaca
     9901 caagttaata aaaccaatga agaattcaaa ttggttaata aaggacctga gattcctcag
     9961 gaacaaatca aaacttttct aaatcaaata caaaagctgc aagctgacga ttatttgtgt
    10021 gtttctggca gtttgccgcg aggggtctca cccgaaattc ttattgatat cagtcgtatt
    10081 tgttttgaaa ataaggtcca tctgattatt gattcaagct atccggaaat aattgattgt
    10141 ctgccgtatc agccgttctt attaaaacca aacgaggagg aattgcaatc ttggtataat
    10201 gtcgagctta gtgatcgtga aggatatatt cattatgctg aacgcttgat tgccgatgga
    10261 gcgaaaaatg ttctcctttc tctaggagga gagggaggaa tatttttaaa taaggatatc
    10321 gtttattctg ggaattctcc taaaggtaaa gtagtcaata cagcgtgtgc tggtgacact
    10381 ttgttgggaa catttcttgc aggatattta aaaggtgaac aggtagatat taatttgaag
    10441 aaaagtttag cagctggaag ttcaaccgct tttcgtaaag gattaacaga tttttcggat
    10501 gttgcagagt tggaaaaaca aatcgaaatc aaagagggag cgagtaaaaa atgacaactt
    10561 acaaaattgt tgccgcaaca ggatgtccaa caggaattgc ccacacttat atggctcaag
    10621 aagcattgga gcaggcagct aagcgcaaag gggtatctat caaagttgaa acccatggtc
    10681 aagtagggat agagaatgaa ttaacaccag aagaaataaa aaacgctgac gctgtaatca
    10741 ttgcagctga caaagatgtt caagcagaac gtttcgcagg taagcgtgta atcgatgtgc
    10801 ctgtcagcaa ggggattaaa gatgctgacc aattgatcga agatgcgttg gctggtaaag
    10861 gttcaatcat tgcagaaaac aaaacagttg acgctgctgg tgagaaagcg gagatggggg
    10921 ttgcagcagg cattggtcac agtatttata aaaatctcat gaacggtgtt tctcatatgc
    10981 tgccgtttgt agtggcaggt ggggtaatta ttgcaatttc gtttgccatc tggggtgtgt
    11041 actcttttga tcccaatgat cctacttaca atccgactgc cgctatgtta aagggtgttg
    11101 gtgatgcggc aatgggaatg atggttccta ttttagcagc ttatatcgct gaaggaatcg
    11161 ctaaacgtcc tggtttggtt gttgggtttg ttggcggact gttagccaat tctggcggag
    11221 ccggtttctt aggcggaatt tttgcaggtt tcttagcagg ctactttatc ttactattgc
    11281 aaaaggtcat gaaaggcttg cctaaacaat tggatggtct gaaggcgatc ttcttatatc
    11341 cggtgatcgg tgtcttgttt gttggtgtag tcatgtcttt attagcaaat ccaatggaag
    11401 cagtcaacca aggaatgatg aatttcttgg caggattcca aaattctaat ccattaattt
    11461 tagggattat cgttggctgt atgtcagcat ttgatatggg aggtccagtc aataaagcgg
    11521 cttatgttac agggactgcg ttgttggcac aaggtaacca tacatttatg gcaggggttt
    11581 cggcagcttg tatcgcgccc ccattgatta ctggatttgc cgtactgttt tttggaaaat
    11641 atttcgacac gaatgatcgc aacgctggtt tagtcaactt tatcttgggc tcaacgcaca
    11701 ttaccgaagg agcaattcca tttgcggcta aagatccgct gcgtgttctg ccaactatga
    11761 tgctaggttc atcaattgct gctgtactga catatatgtt tggtgtccaa gttcccgcac
    11821 ctcatggagg ttttctagta ttacctgttg ttactggtaa ggttgcatgg gtagggtcga
    11881 ttttacttgg ttcaatcgtt ggcggagtaa ttttagggtt gatccaaaaa cgtagtgtag
    11941 aaaaacgtga agcagaagca gctactgttg aaatgaaaga agaaaccaac aactaaaggt
    12001 tattctagaa agaaggattt caaatgactt tgatcaaaac aaataaagtt tttatgaata
    12061 aaaattttac gacgcaagat gaggtaatga acttccttgc agatcagtca attgtcttga
    12121 gcattagtga agaccgagat gcagtttacg aaaaattgat ggaaagagaa gaagaaagca
    12181 cgactggtat gatggatggt tttgccatcc ctcatgccaa agatgctagt atcaaggatg
    12241 ccgatattgt gattgttcgg ttaaacggta aggtcgattg gaaaagttta gatggagaag
    12301 gaacggattt tgtaatcgct ctgtttattc cagattcaga ggctggaaca acccatctaa
    12361 aatttttatc tcaggtagcc agacttttga tgcacaaaga tgtgacagaa ggtctgaaac
    12421 aagcccagtc tgctgaagaa atagctgaac ttttaaacca aaaattagaa gaaaactaaa
    12481 aaggaagtag tgaaatcatg cctttaacag aaaaaaagaa aacttatatt cgtcaactaa
    12541 gtaatgaaag tggcatcatc agtgcgttag caatcgatca acgtggtgcc ttgaaaaaaa
    12601 tgatcggtaa atttcaaaat caatcagcaa ccgatgaaca aatcatcaat ttcaaaaaaa
    12661 tcatttcttc tgaattgact ccatatgctt cagcaatctt attagatcca gaatatgggt
    12721 tgccggcagc cgaagttcgc gataaaaatg ctggtctgtt gttggcctat gaaaaaactg
    12781 gttacgatgc ttcaactcca ggacgtttac cggatatttt atctatttgg tctgtaaaac
    12841 gtctaaagga gcaaggggcc gatgcttgta agttcttgtt atactacgat gtagatgaat
    12901 ctgatgatat caatgaccaa aagaaggcgt ttatggagcg gattggttct gaatgtgttg
    12961 ctgaagattt gccatttttc ttagaattgg tttcttatga tgccaacgat cctgatactt
    13021 cttcgaagga atatgccttg aaaaaaccgc ataaagtaaa tgaaatgatg aaggaatttt
    13081 ctaagcctcg ctatggagtt gacgttttga aagtggaagt tcctgttaat atgaactatg
    13141 tggatggtta cactaaaaat gaagttgctt ataccaaaga agaagctgct gcttatttca
    13201 aagaacaatc cgagactacc aacctgccat ttattttctt gagcgcagga gtaactgctg
    13261 aattgttcca agaaaccttg aaatttgcaa aagaggctgg ctctacattc aatggggtac
    13321 tctgtgggcg cgcaacttgg gcgaatggtg tagaaccatt cgttactaaa ggtgaatatg
    13381 ctgccaaaga atggatgcag acacaaggac gcaagaacat cgaagagctg aataatgttt
    13441 tgaaagaaac agcaacagct attaaattgt aagaaagctt taactataac cttaagtttg
    13501 taacctaatg atttttaata atcaatttga gcttttaaat aaagaattgg aagcgtcccg
    13561 acgcttcctt tttcgtttgt cttaaggcat gataggttct atgttgtcgt tcaataagcg
    13621 caagaaggtc agcatttttt tcttagagta tgcaaaattg ctttctaccc aatgatttaa
    13681 aacggagagg cacccttgga tatggtaaat ggtgaagtaa cgcatttctt ctgtgatcac
    13741 ggtttttttg tcttcttgta gccagatttt taaataattt tcttcgatca gattatttaa
    13801 tctcttttgg aattggatat ctccgtggtt gataacgaat aacttgtaca cgaacgtatt
    13861 tgtatagaca tagtcgatca aatgagcgta tagatcctcg tagctgtgtg tcgtgtcata
    13921 ggaaatgatt tcatccaagt cttagaaaaa gctattttct agcttttcat aaagatcata
    13981 gatatccgta taatgtttat aaaatgtcga acggtgaaga tcagcagttt cggtcagttc
    14041 acggatagtg atgttttgaa tttttcgcga attgtaaaat taagttagaa aaataaaaag
    14101 gcatttatgg tacactcaaa ttgtatttcc gacgaaagaa aacaaaggag tgtaaccata
    14161 aatgacctac a
//
LOCUS       DAIGOK010000070        14146 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.070, whole genome shotgun sequence.
ACCESSION   DAIGOK010000070 DAIGOK010000000
VERSION     DAIGOK010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.070"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(23..3697)
                     /gene="rpoC"
                     /locus_tag="NOA01_001699"
     CDS             complement(23..3697)
                     /gene="rpoC"
                     /locus_tag="NOA01_001699"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCI0632888.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(3755..7372)
                     /gene="rpoB"
                     /locus_tag="NOA01_001700"
     CDS             complement(3755..7372)
                     /gene="rpoB"
                     /locus_tag="NOA01_001700"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCI0632889.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            8100..9068
                     /locus_tag="NOA01_001701"
     CDS             8100..9068
                     /locus_tag="NOA01_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCI0632890.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            9086..9991
                     /locus_tag="NOA01_001702"
     CDS             9086..9991
                     /locus_tag="NOA01_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI0632891.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            9988..10794
                     /locus_tag="NOA01_001703"
     CDS             9988..10794
                     /locus_tag="NOA01_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCI0632892.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            10930..11919
                     /locus_tag="NOA01_001704"
     CDS             10930..11919
                     /locus_tag="NOA01_001704"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HCI0632893.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(12236..12760)
                     /locus_tag="NOA01_001705"
     CDS             complement(12236..12760)
                     /locus_tag="NOA01_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HCI0632894.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            13047..>14146
                     /gene="scm"
                     /locus_tag="NOA01_001706"
     CDS             13047..>14146
                     /gene="scm"
                     /locus_tag="NOA01_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HCI0632895.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTT"
BASE COUNT         4005 a         3059 c         2789 g         4293 t
ORIGIN      
        1 cacaacctct tttttaaaat atctattttg tttgattgtt cattgcgtct tctgcagcca
       61 tttgcgcttc aatatctgaa atactgtaga cattttcgct tgctacaccg acttcttttg
      121 gttccatgtc acgataacgt tgcataccag taccagctgg gatgatctta cctataataa
      181 cattttcttt caaaccaaga agaggatctt tcttaccgcg gatcgcagca tccgtcaaga
      241 cacgagttgt ttcttggaat gatgcagcag ataagaaact atttgtttct aatgatgctt
      301 ttgtgatacc tagtaagaca ggtcgagaag ttgcaggaac gcctcctgag actagcgtat
      361 tgtagttttg atctttaaag tctgcgatgt ctaataatgt acctggtaag atatctgtat
      421 cacctggatc cattacacga actttacgca gcatttgacg aaccattact tcgatatgct
      481 tgtcgccgat ttctacccct tgcatacggt aaacacgttg tacttcacga agcaagtagt
      541 tttcaacaga taatacatcg cgaacttgca acaattgttt tggatcgatt gatccttctg
      601 tcaatggtgc accacggtgg atcatatcgc cttctgctac tttcatacgt gctgtataag
      661 gaacagtata cgtacgtgta tctgtttttc ctttgattgt tacttctttg gtacgtgttg
      721 caggatcttc tgagatatcg atgacatccc ctgttacttc tgtgatcact gcttgccctt
      781 tcggattacg tgcttcaaag atttcttgga cacgaggaag accttgagtg atatcgtctc
      841 cggcaacccc acctgtatgg aatgtacgca tcgtcaactg agtaccaggt tcaccgattg
      901 attgagcggc gatcgtacca actgcttctc cgacttcaac gtcagagcca gttgcaaggt
      961 tgcgtccgta acaatgtttg cagacaccat gttttgtatt acatgtgaag acagaacgga
     1021 ttgtcactgt ttcgacacct gcatcaacga tttgttttgc gatgtcttcg gaaatcaatt
     1081 ggtttcctgc aatgatcaat tcatttgttt caggatgacg tacttcttta cgggtgtaac
     1141 gtcccactaa acgatcttct aatgattcga tgatttcatt tccctcgcgg atggcttgga
     1201 tttcaagacc tcggtcagta ccgcaatctt cttcacggat gattacatct tgggccacgt
     1261 caaccagacg acgagtcaag taacctgaat cggcagtctt caaggctgta tcggtcatcc
     1321 ctttacgagc cccgtgagtt gagataaaca tttccaagac ggatagtcct tcacggaagt
     1381 tcgaaatgat tggcaattcc atgattcgac cattaggagc ggccatcaaa ccacgcattc
     1441 cggcaagctg tgtaaagttg gaaatgttac cacgagctcc agaatcagac atcatgaaga
     1501 ttggattttt cgcttccaaa ccttccatca gtttttgttg gatgctgtct tttgcagcat
     1561 tccaaacagc aatgacacgt tcataacgtt cgtcatctgt gatcagacca cgacggaatt
     1621 gttttgtaat agtttcaact tgtttatggg cattttcaat gatttcttgt ttttcattca
     1681 gtacactgat gtcagcgatc cctactgtga taccggcatg tgtcgaatgt ttgtacccta
     1741 agtctttcat acggtcaagc atcttagaag tttctgttac tttgaatcgt ttgaagactt
     1801 ctgcgatgat gtttcctaag tttttcttct tgaacggtaa tactaattct tgctctttga
     1861 tatgtgcagg aatatcagta ccagcttcaa caaagtattt gtccggtgtt tggacggtca
     1921 agttgaagtc tgttggttca ttcagataag ggaattcagg aggcatgatc tcgttgaaga
     1981 tgatcttacc aactgttgtg atcatcgtac gttctttttg ccattcagta aatggttttt
     2041 cacctaatga gttagggttc acaccgatac gtgaatgtaa atgaacatat ccgttgcgcc
     2101 atgcgataac ggcttcgttt tgatctctga agaccattcc ttcgccttcg cgtccttctt
     2161 cttccattgt caagtagtag ttacctagaa ccatatcttg agaaggagta acaactggtt
     2221 taccatcttt agggttcaag atgttttgag cagctaacat caacatacgt gcttctgctt
     2281 gtgcttcttc attcaatgga acgtggacag ccatttggtc tccatcgaaa tcggcattat
     2341 aagcttcaca tacaagtgga tgcaaacgga ttgcgcgtcc ttgtactaaa actggttcga
     2401 atgcttggat accaagtcgg tgaagcgtag gtgcccggtt cagcaatact gggtgttcct
     2461 tgattacgtc ttctaaaaca tcccatactt cgtcttcttg acgttcgatt ttacgtttcg
     2521 catttttgat gtttgaagct agttcgcgtt gaacaagctc acgcatcacg aatggtttga
     2581 ataattcaat ggccatttct tttggaagtc cacattggta catcttcaag aaaggtccaa
     2641 caacgataac ggaacgacct gagtagtcga cacgtttacc tagcaagttt tgacggaaac
     2701 gaccttgttt ccctttcaac atatgagaaa gagatttcaa tgggcggtta cctggtccag
     2761 taactggacg tccgcgacga ccgttatcga tcaatgcatc tactgcttct tgcagcatac
     2821 gtttttcatt ttgaacgatg attccaggtg cattcaagtc taacaaacgt ttcaaacggt
     2881 tattacggtt gattacacga cggtaaagat cattcaagtc acttgttgca aaacgtccac
     2941 cttctaattg aaccattggg cgtaaatctg gtggaataac agggataacg tccataacca
     3001 tccagcttgg ttgatttcct gaagcgcgga atgcttctaa aatatccaaa cggcggattg
     3061 cacgtgtacg tttttgtcct tgtgcagatt tcaattcttc tttcaattcg gctgcttctg
     3121 catctagatc aacattatca agtaattgtt taatcgcttc tgcacccata gcagcttgga
     3181 attcttgtcc atattgttca cgtttttcgc gatattcgcg ttctgtcaac aattgttttt
     3241 tctctaaatt cgtatttcct ggttcgatga ctacatatga agcaaaataa atgatttctt
     3301 ccaaagcgcg tgggctcatg tctaatacaa gacccatacg tgaaggaatc cctttaaagt
     3361 accagatatg tgaaacagga gctgctaatt caatatgtcc catgcgttca cggcgaactt
     3421 tcgaacgagt aacttccact ccgcatcgat cacaaacgat tcctttataa cggatacgtt
     3481 tgtattttcc gcaggcacat tcccagtctt ttgtaggacc aaaaatccgc tcacagaaca
     3541 acccttcgcg ttcaggtttt aaggtacgat agttaatcgt ctcaggtttt tttacttcac
     3601 cgtaagacca gcttctgatt ttttcgggag aagccaaacc tatttgcata ctttcgaatt
     3661 tatttacatc gatcaaaagg ggttccctcc atttcattta atggcaaacc gcaagacggc
     3721 tgatcttcga ccgccttgca gctcatagac cggattagtc taaattatct tctgcggttt
     3781 caaagttttg gatgacatcg tctttttcat cttccacttg ttcagcagct ttcttttcta
     3841 attctttggc agtttgctgt tcggcaaatt ttgtcaaagc atcaacagtg atcaaatcgt
     3901 cgtcttcatc gtccatgtcg cgaagttcga tctctgtttc ttcgatatcc aagacacgca
     3961 tgtccagacc tagtgattgc aattctttca ccaatacgcg gaatgattcc ggtacacctg
     4021 gttttggaat tggttcaccc ttgacgattg cttcgtatgt tttcacacgt ccgactacgt
     4081 catctgattt gtatgtcaag atttcttgta aagtataagc cgcaccgtaa gcttccagtg
     4141 cccatacttc catttcacca aaacgttgtc caccaaattg agctttacct cccaatggtt
     4201 gttgtgtaac caatgagtaa ggtccgattg aacgtgcatg caatttgtca tcgaccatgt
     4261 gagcaagttt gatcatgtac atcacaccga cagacacacg tccatcaaat ggttcacctg
     4321 tacgtccgtc gtacagaaca gttttcgcat cgctggccat accggcttca cgaactgttt
     4381 cccatacgtc ttcatcactt gcaccatcaa atactggcgt tgctacgtgg atacctaact
     4441 gacgtgcagc catacccaag tgtaattcca atacttgccc gatgttcata cgtgaaggca
     4501 cccctaaagg attcagcatg atatcgattg gtgttccatc aggtaggaat ggcatatctt
     4561 cttccggcat aatacgggat acaacccctt tatttccgtg acgaccggcc attttatccc
     4621 cttcatggat tttacgtttt tgaacgatat aaacacgaac taacatgttt acgcctggtg
     4681 acaattcatc tccagcttca cgagtaaaga ttttcacatc gtgaacgata ccgccgccgc
     4741 catgtggtac acgtaatgat gtatcgcgga cttcacgagc tttttcgcca aagatcgcat
     4801 gcaatagacg ttcttctgca gataattctg ttaccccttt tggcgtgact ttccctacaa
     4861 gcaaatcgcc atcttggact tctgcaccaa tacggatgat acccatttcg tctaaatctt
     4921 ttaatgcgtc ttccccgaca ttagggattt cgcgtgtgat ttcttcaggt cctaattttg
     4981 tatcgcgggc ttctgattca tattcttcaa tatggataga agtgtatacg tcatctttta
     5041 ccaaacgacg gctcatgatg atcgcatctt catagttgta cccttcccaa gtcatgaaac
     5101 caactaaaac gttttggcct agagccattt cgccttgttc catagatggt ccgtctgcca
     5161 gagtatcccc tttttcgact ttctcaccca aatgaacgat tggacgttgg ttgtagcttg
     5221 ttccggagtt tgaacgacgg aattttgtta catcgtattt atctaatgca ccgttgtcgc
     5281 ggcgtacgcg gatttcagaa gcatcaacgt attcaacgac accgtcatgt ttacataaca
     5341 aagcagctcc tgagtcatgc gctgatttgt attccatacc agtaccaacc catggtgctt
     5401 gaggattgat caacggcact gcttgacgct gcatgttggc acccatcaag gcacggttgg
     5461 agtcatcgtt ttccaagaaa ggaatacatg ctgtcgcaac ggcaactact tgtttaggtg
     5521 atacgtccat gtagtctact ttatcaatag atacttctaa gttttcactt tgagcacgag
     5581 ccataacaac gtcttgagca aatgttccat cttcattcaa tgggctgttt gcttgggcta
     5641 cgatgtaatg gtcttcgata tcagctgtca agtagtcgat ttggtcagta acacgacctg
     5701 tttcacggtc tacacgacga taaggtgttt cgataaatcc gaatttattt actttcgcat
     5761 aactggacaa actattgatc aacccgatgt ttgggccttc aggagtttcg atcggacaca
     5821 tacggccata gtgggagtag tgaacgtcac gaacttcata accggcgcgg tcacgagtca
     5881 atccgccagg tcctaaggct gaaagacgac gtttgtgtgt caattcgcca agagggtttg
     5941 tttgatccat gaactgtgac aactgagaag aaccaaagaa ttctttgata cttgccacaa
     6001 ctggacggat attgatcaat tgttgaggtg tcaatgtttc agtatcttgg atcgacattc
     6061 tttcacgaac cacacgttcc atacgtgcta gcccgatacg gaattggttt tgtaaaagtt
     6121 caccgactga acggatacga cgatttccta agtggtcgat atcatcgaca ttgccgatac
     6181 cttccatcaa gttcaagaaa tagctcattg aagcaatgat gtcggctgga cgaaccgttt
     6241 ttacagctgc ttctggatag ccgtttccga tcacgtttac ttcacgttca gggtctcttg
     6301 gtgaaaatac tttgatgact tggacagtca ttggatctgt gaccacaccg tcttctgatg
     6361 gatagtaagt cactgaattt aggccattat caatgaaagg agctagtgtt tccatgactt
     6421 ggtgagtcag aactgttcct ttttcaacga tgatctcgcc tgtttctgga tcaactaatg
     6481 tttcagctaa tgtcagattc aatagacgtg ttttcaaatc taattttttg tttactttgt
     6541 aacgaccaac gtttgctaag tcgtaacgtt ttggatcgaa gaaacgagca ttcagcaagt
     6601 tacgtgagct gtctgctgtt tttggttcgc ctggacgcaa acgttcgtaa acgtctttca
     6661 aaccttcttc tgttcttgag tcgcttgcgt ttttatgcaa gtctttttcg actgtattgc
     6721 gtaatgtttc gctgtcgccg aagatttcaa agatcgtatc atcggaacca aatcctaacg
     6781 cacgaactaa tacggttaaa ggaatcttac gtgtacggtc gatacgtaca taagagatgt
     6841 cttttgcatc agtttccatt tctagccaag ctccacggtt agggataaca gtagaaccga
     6901 atccttcttt tccgtttttg tcgacttttc catggaaata gacacctggt gaacggacta
     6961 actgagaaac gattactcgc tctgccccgt tgatgatgaa tgttccttgt tctgtcatta
     7021 atgggaaatc accgaagaat acttcttgtg ctttgatttc gccagtctcg cggtttgtca
     7081 aacgcaatgt tacatgtaat ggtgcagagt agtttgcatc atgcgcacga gcttctgcta
     7141 ctgtatactt tggttctttc aattcataat ctacaaattc caaagataga ttgccattga
     7201 agtcatcgat tggcaaaatg tcttcaaaca tttctctaag tccctcatct aagaaccatt
     7261 ggtaagagtc tgtttgaatt tcgatcaaat ttggtaattc caacacttca ctgattcttg
     7321 cgaaacttct acgttcgcga tgttttccgt attttactac gtgtccagcc aagctcttca
     7381 cccctctaaa atgtcattca aaaagactac ccgatcaatt gttacaataa tatttcaaat
     7441 attattttta ggtaacaaca gacatttttt gcggctttta ggcaaaaaaa aaccaaaaat
     7501 agaacattct cttgaaacct tacttcgtga gcttctactt ttgtttcctt atattaaagc
     7561 cgaaacggac aatatttcgt ttctgccttt tcttcatcag atagtttttg caacttcata
     7621 tactacactt attttctata aaagtcaaca cttcctggcg tttcttctgc tttttcgcaa
     7681 cattttctta atgttttcaa ttttatgttt tcaattatct gacaattctt aattgacaga
     7741 cctttttatc tcggttatac tttagaaaat tcattttgtg gaggaacttc tatgcgaacc
     7801 aacatcaacc aaatcgcacc aacacaactg aataactttt actttagcta ctttagatag
     7861 gtttgtattc atcatgatgg atacaaacgt taaacgacag tttgtatcta tgatggctaa
     7921 agaactttgt attgcagcaa aaagccacgt agacaaaatg ctaagtggct gagcagtgaa
     7981 aatacaaagt aaaagtatca agcattctac tttttcaatt tattttccga cttttcaatc
     8041 catttggaca ttttgtctga atggattttt tgtttcccaa aattaaggag gaaaaagaaa
     8101 tgaaaaaaaa tataatcatc ggattaggcg ctgccctgct actagcttta ggaggttgcg
     8161 cacaaaaaga caattccgca gaggcatcag atggacaaaa aacaattggc gtattgcaac
     8221 ttgtacaaca taactcgtta gatgctgcct ataaaggatt caaagaagga ttagcagaag
     8281 aagggtataa agaaggcgat aatctaacaa tcgattatca aaatgcccaa aacaatcaag
     8341 acaacctgaa aagtatgagt gagaaactcg tcaaagaaga tcctgatttg ctacttggaa
     8401 tcgccactcc agcagcacaa tctttattaa atgaaacaac cactatcccg atgactgtca
     8461 cagcggtcac agacttggaa caagcccatt tagtcacatc caatgaaaaa cctggcggca
     8521 atgtgactgg cacgacagat atggtgccaa tcgaaaaaca aatcggctta ctgttaaata
     8581 tcgtgccaga cgccaaaaca atcggcatca tgtataacaa cggtgaagca aactctaaga
     8641 tccaagctga tttggcagaa aaagaattga aaaaagcggg cgtcaaagtc aaagtactga
     8701 cagcaaattc cacaaatgat gtccaacaag tgacgaaaag tctagctaaa gatgtagatg
     8761 gaatctatat tcctactgat aacacgttcg caagtgcggc aagtgttgtc ggtgaagtcg
     8821 caaaagaaac aaaaacacct gtcgttgctg gcgcagttga tcaagtcaaa gacggcggtc
     8881 tagcaacagt aggaatcgac tacgaaagct tgggcaaaca aacagggatc atggcagcaa
     8941 aaatcttaga cggcaagaat ccaagtgaac ttcctgtcga aacagcgaat gatttggaac
     9001 tgtatgtcaa taaagacatg gccaaagcat taggcatcaa tccagacagc atcaaagaag
     9061 ctgaataaaa cacaggagga ttctcatgac aaatttgctc tctcttttct tatcatctgc
     9121 atcgcaaggg gtcttatggg ccctacttgc gattggtgta tacgtcactt tccgtatctt
     9181 ggacattgcc gacttgactg ctgaaggaag ctttccttta ggtgcaggga tcgcggcagt
     9241 aagtattaca aatggctatc atccattgac agcttgtctt ctaggctttt tagggggtgc
     9301 ggcagcaggg cttgtttccg gactgcttca cacaaaattg aaaattcccg cccttcttgc
     9361 agggatcatc accatgactg gtttatattc tgtcacatcc cgtgtgatgg gtgcgccgaa
     9421 catctcttta ttgggtcagc caacgatctt tcattgggtc gagtcattcg gcataacaaa
     9481 agacaacgct gtactgatcg tcggtctgct ttttgctttg atcgttgtat gtttacttgt
     9541 tttgttctta cgtacagaaa caggacttgc tattcgtgca actggcgata atctggcaat
     9601 gagcgaagca aacgggatca acacagataa tatgaagatc atcggttata tgatttctaa
     9661 tggatgcatt gctctttctg gctccttact tgcgcaaaac aacggctttg ctgatttaaa
     9721 ttctggtatt ggaacgatcg taattggact tgcttctatt atcatcgcag aagttttgtt
     9781 acgtaaccag ccactatttc tccgtctctt gactgtcgtt ttaggtgcgg tcatttatcg
     9841 ttttatttta gcgttagtct tcgaactgaa tgttgaacca agtgattcga aacttgcttc
     9901 tgctttcgtg ctagtgatct gtctttcttt cccgcagatc cagcaaaaat tccatttacc
     9961 tcaaaaaaag aaaggcggta gtgtcaaatg aatgacgtct taacattgga aaatctccat
    10021 catacctttg aaaaaggaac gatcaacgaa aatcatgtcc tgaaaggcat taatctttcc
    10081 ttagatcccg gcgacttcgt gacgatcatc ggtggaaatg gggctggtaa atctactttg
    10141 ctgaacagca tcgccggtac attttctgtc gatcagggtc aaattttatt gaatggaaaa
    10201 aatatcacga aaaaatctgt tgtcgaacgt tcgaaatcca tcagtcgcgt attccaagat
    10261 cctaaactag ggacagccgt tcgtttaacg gtagaagaaa atcttgctct tgctatgaaa
    10321 cgaggaaaaa aacgcggctt cttccgtggt gtcaaaccac aagaccgaac atttttcaaa
    10381 gaccagctgg ttcggctaaa tttggggttg gaaaatcgtt tatcctcaga aatcggtttg
    10441 ctgtctggag gacaacgtca agctattacg ttattgatgg ccacattaca gcgtccagaa
    10501 ctgatcttat tagatgaaca cactgcagcg cttgaccctg ctacttccaa aacggtgatg
    10561 gaattgacag aaaagctgat cactgagcag aatctcactg cttttatggt cactcatgac
    10621 atggaagacg cgatccgcta tgggagtcga ctgatcatgc tgcatcaagg aaggatcgtc
    10681 gtagatatcc agcaagagga aaaacagcag ctcacagtgc cccaattatt agcgttgttc
    10741 aaacaaaaca gcggcacggc attaaaagac gatcaagtac ttttatcttc ataatcagaa
    10801 agaggatggg acaaaaaatg aacgcccacc ctcttctttt ttctctttcc ttctttcgtt
    10861 tttgctttat gtatcaaccg ttgtttcttc gaaaactatt ttgttctctc cttcttcctg
    10921 atagaatgga tgggaaagga gcttgatgcc atgacaacga aagacaaggt acttcaatta
    10981 ttgaaaaaga caccagattt tctttccgga gaaaagttgg cacagcaact ggaagtttcc
    11041 cggacaagta tttggaaagc catcaaagaa ttggaaaagg caggctatcg ttttgagcac
    11101 gggcctacag ggtatcgtta cctgccctct gacgtactag atgcaaaaga aatccagcaa
    11161 gagctacggt cgtttattcc tgatttgaaa gtacaaacga tgttgacttc tgaatctacg
    11221 atgaaagatg ccaaactggc agcagctcaa ggagattctg tttcgaagtt atttgttgct
    11281 gatacacaag aacaagctcg tggacgtttt ggacggcctt ttttcgctga acctggacga
    11341 ggaatctaca tgagtctctt gctccatcca gacaaacggt ttgatgaatt gcctcaatat
    11401 acgatcatcg cagccagtgc ctgtgtgaaa gccattgaat ctgtaaccgg tcgttctgct
    11461 tcgatcaaat gggtcaatga tatctacatg gatggaaaaa aagtctgtgg gattttatct
    11521 gaagcagtca gtgatatgga aagcggcagg atcagtcata tcatcattgg gatcgggctt
    11581 aatttttctg ttcctcaaaa tcattttcca gctgatctcc aagaaaaagc catctctgtc
    11641 tttcccgacg gacaagctag tgtcacaagg aatcagctaa tcagcaaaat ctggcaagaa
    11701 ttgttcactt atctttctca cccagaaaaa gcgttggact tctataaaga aaaatcattc
    11761 gtattgggaa aaaccgtgtc gttcaatcaa aaaggaacga cttatgtcgg tcttgcaaaa
    11821 gaaatcggtg atcatggcga actgatcgtc caaacaaatg aacagaagat gagtttgtct
    11881 tctggcgaaa tcagtctcac atctatccgt acgcaataat tatcagattt cacaaaagga
    11941 cacctgatct catacagtgt ccttttttct gaattctctt caaaagactc tccttattct
    12001 atcagctgac agtttctcta tcctctagaa aaaggtattc taaaaatatc tataaagcat
    12061 ggtacacttt ctttaaatga atcaatgcaa aaaacaagag agtcatcagt ttacacgatc
    12121 aaaagagata gcaggccgct tcctttttca atcttataga tagaccatgg gtaacgataa
    12181 aaacaaaaaa gaacttgcga cttattttgc cgtaagttct tttttagcta gtgccttaat
    12241 ctcttaatcc caacaaacgt ctcgcataga tcacctgacc agagtgcata gcagcatcat
    12301 caataataga aacgagacgc attcctcgtg tgactggtgg tgtccagttc tcgtcaatta
    12361 cttcatctag ctcggaaggt tccagccttc ttaagtaaga aatagcaaaa tcagcagctc
    12421 ctcgcaagta tcctagcaga tcttctattt gattagcttg gatctgcttt gcttgttcca
    12481 gactatgctt ccaatcttgt tcgttatctg ctatcgtcat cggaaaccgg ctttcccagt
    12541 cttgactatg ccaaattgga gaagttcctg ctaaatcagc aatctggaaa tctaattctc
    12601 gagcggtatg ccaagccagc caagtcattg atttgatctg cggggctcta tcttccactg
    12661 gaaaacgatt ggcctcctca atagagacgc cttcaaacaa acgttcaaac cgatcgtatg
    12721 cccgatccac actctcgcat agcatatcta aataatccat ttattttcct cctttttatt
    12781 tatcctactt actttttcct aattctagga tacctactta tatgttacta cacaccctta
    12841 ttttttacac tttaacagtc accatcatca atagtttgga gattgaaaga aaaagatagt
    12901 gtcgaactat caattgaaca agaagcaaca atatgaacgt ttcattaata ttctttaaat
    12961 aaacaatgat ttttcaaacg aacagtttaa gactaggaga aaaagagtat aataggaaaa
    13021 tgtttgaaag gaaaggaagg ggaaaaatga aaaaaagggc aagtttacta gtcctagttg
    13081 ctttgttgtt caatatcttt tcgtatggtg tggccgtttc agcggaaaca tacattccta
    13141 aagaccctgg gaatatttat aatgatctaa ctggtaacta tggcttgtta ggaattgcta
    13201 gtcaattcca cgtatttgct aaaaataaga caactgtgaa tgcacataca gacggaaata
    13261 tagcaacaca tgaacttgat gcaaaaaata attttggtac agagcttcat gaaggtttat
    13321 tgaaccaaga aataaattat atacaaaaaa ctgattcttt cgctaattct tcaggcatac
    13381 ctacaggtag cacaagaatg aataaatttg tcgttggcag taacgtggat gtagcacttc
    13441 aagatggtca tcctacagtc aatggatcca ggcttgatca cttaactcca gatgattttt
    13501 ataaagatcg tgctgggcat gaatatattg atttcgagca ggaatttgct aaattgaatg
    13561 tagcagcaaa tgatcttgca acgatcactc cagctaaaac atatactgct gcagattttc
    13621 cagacatgaa caatcggaca atcgatttaa ctgatttatc agataccggg tttttattgg
    13681 taaatatcga tgcagaagtg ttaacgatga atacgccact tcaaatcatt aatccaaatg
    13741 atcaagtagt tgtatttaat gtcatcaaca gttcttctgc attgaacgta cagtcaccaa
    13801 tcaaatacaa tgatcgttca aatcatgaaa cagaagattt ttcggatgcg aatatctctt
    13861 ggaattttgg aaatgagatg actgatttga caatcagtgc tccttttcaa ggaacaatcc
    13921 ttgcgcctaa tgctactatc cgcattactc aaaaccaaga cggaacgatt ataggaacaa
    13981 atgtgatttt agatgcagct accaatcgtt gggatcctaa cgaaatattt atcagcgaca
    14041 atggtacaga tacgacagac acaagcgaca gtacagatac atctaccaca agtgatagct
    14101 cagattcatc aacttcaagc gacagtacag atactacctc taccac
//
LOCUS       DAIGOK010000071        13938 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.071, whole genome shotgun sequence.
ACCESSION   DAIGOK010000071 DAIGOK010000000
VERSION     DAIGOK010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13938
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.071"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(<1..244)
                     /locus_tag="NOA01_001707"
     CDS             complement(<1..244)
                     /locus_tag="NOA01_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCI0632896.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAF"
     gene            complement(723..1442)
                     /locus_tag="NOA01_001708"
     CDS             complement(723..1442)
                     /locus_tag="NOA01_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCI0632897.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(1491..3137)
                     /locus_tag="NOA01_001709"
     CDS             complement(1491..3137)
                     /locus_tag="NOA01_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCI0632898.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            3316..3906
                     /locus_tag="NOA01_001710"
     CDS             3316..3906
                     /locus_tag="NOA01_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCI0632899.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            3998..5503
                     /locus_tag="NOA01_001711"
     CDS             3998..5503
                     /locus_tag="NOA01_001711"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HCI0632900.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(5587..6939)
                     /gene="celB"
                     /locus_tag="NOA01_001712"
     CDS             complement(5587..6939)
                     /gene="celB"
                     /locus_tag="NOA01_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCI0632901.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(6939..7457)
                     /locus_tag="NOA01_001713"
     CDS             complement(6939..7457)
                     /locus_tag="NOA01_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632902.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(7473..7799)
                     /locus_tag="NOA01_001714"
     CDS             complement(7473..7799)
                     /locus_tag="NOA01_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HCI0632903.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(7812..9791)
                     /locus_tag="NOA01_001715"
     CDS             complement(7812..9791)
                     /locus_tag="NOA01_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCI0632904.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(9812..10126)
                     /locus_tag="NOA01_001716"
     CDS             complement(9812..10126)
                     /locus_tag="NOA01_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HCI0632905.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            10294..10587
                     /locus_tag="NOA01_001717"
     CDS             10294..10587
                     /locus_tag="NOA01_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCI0632906.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(10665..11777)
                     /locus_tag="NOA01_001718"
     CDS             complement(10665..11777)
                     /locus_tag="NOA01_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCI0632907.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            12164..12343
                     /locus_tag="NOA01_001719"
     CDS             12164..12343
                     /locus_tag="NOA01_001719"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632908.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            12537..12707
                     /locus_tag="NOA01_001720"
     CDS             12537..12707
                     /locus_tag="NOA01_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCI0632909.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(13131..13871)
                     /locus_tag="NOA01_001721"
     CDS             complement(13131..13871)
                     /locus_tag="NOA01_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCI0632910.1"
                     /translation="MTKANRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGL
                     TTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTP
                     GGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVET
                     QAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTGE
                     TIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT         4356 a         2849 c         2383 g         4350 t
ORIGIN      
        1 gaaatgcttg atccgaagaa aataaaaatt tgaaattttc ccaacccacc caaggacttt
       61 ccctcaagct agccaagaac ccatctgctg aaatatgaaa atttttgaat gctacgacat
      121 tcgccattac aggtatgtaa aagaaaaaaa tcatccaaag catacccggt acagccatca
      181 ggacaagtgc tttataacgc catagattca taaaaaaatg attgaacccc tttcgttttt
      241 tcatctaaaa acccctttcg attcattcgc aacgtaatta tagcgctttc aataatgaga
      301 ataaataaac aaagtatagg gaaaatgaaa caaagtatag gagaggttgt gaaatcagcg
      361 ttttggcctg agtattagag aaaaaaacgg gaaaatagtt cctcatattt tgccgttttt
      421 tgagctaata ccgagggtca gctgattgaa caccgtttat cagaggttgt gaattcagcg
      481 ttttggcctg agtattaggg agatcgtgtc cttactatct aactctgagt cacaaggaac
      541 aatggggact gtaacaaaag tcttgtcaca gtcccttatt ccgaacaaac ggtgttccag
      601 aagtagcttc tacggaaata agccgaaatt tcacaaaaaa gccatccgaa agtcagacgg
      661 acaagtctgc ttttcagatg gcgctacaac tactctgctt attcttttag ctatccattc
      721 aattattttt ctctcacttg ttctagctct tctttggtca tgggtcgata ttgcccttta
      781 ttcaaatctt taggcagcca aaattttccc atacggattc tttttaaata taagacttct
      841 tttcctactg ctttgaccat tcgcttaact tggtgaaact ttccttcatg caaaatcaga
      901 cggataattg aagtttctgt ctcttgatct acttgatcaa tttggagttt tgccggcaat
      961 gtctgctcat cgccatcaat aatcagtcct tctgcaaata aacggatatc ttcttctgtc
     1021 attataccgc taacctcagc tagatattct ttctccacat gttttttagg tgatagtaac
     1081 tgatgggcaa acaccccgtc attcgttaaa atcaagaggc cctctgtgtc tttatctaat
     1141 cggccgactg gaaaaagatc ttcccgataa tcctggtctt ccaataaatc gattacggtt
     1201 tgatcatgat tatcctgagt agcagaaatg actcctgcag gtttatgcaa aatgtagtaa
     1261 aaatcttttt gatacgtaat gatttctcct aaataagtaa ccgcatcttc gaattcattt
     1321 acttgcgctt tatcgctttt caccacttgt ccattgactt cgatcgttcc ttttttgatt
     1381 aatcctttga cttctttgcg gctgccaagt cccatatctg ctaaaaactt atctaaacgc
     1441 atgcgtttcc cccatttcaa aagagcatca atctagctct tctgattcac ttattttatt
     1501 tttaatttat gtcgtaaacg cgctattcct ggtcctagta gtctttctgc caagcggatt
     1561 ttcaaagcta aataaccata cactaaagcc cctatacctg ctactacaag aatcaacaat
     1621 agagactgga atttgctatc ttcgtttaaa aaagcaccaa aaatcatctt tgtgatccac
     1681 gaagccaaaa gcataatcaa ggtaagaata aagatcagca atgtcctgcg ccagacaaaa
     1741 cttggtttaa agcgggctac ttcatggatt ctctttaaga tcaaggtaca agacacagca
     1801 aatccgatca ttgtcgccaa taaagggccg tatacttcaa ataaacgaat ggctgggtat
     1861 tgaagaagga gtttcactag aaacccaata accaaataat taattgccgc cttgttttca
     1921 aacattcctt gcagcatgtt ggaaactagc atatatagtc ctaggaagag tcctacaaaa
     1981 cttgcttgga tcagtacatt acttcctaga gaatccggtg tataaaataa tgtatttagc
     2041 ggataggcaa gtaacataac accaaatgtc gccggaaaca taaaaaatga aaacaactgt
     2101 aaatttccgc ttgtcaattt tgccagacct actcgatctt tcaacgtaac agcttctgta
     2161 atcaacggta acccagtaga cgcgatcgat gttgccaatg caataactac catcgtcaac
     2221 ttatctggat tggcactaaa aatcgaaaaa agatccagta actgtgcatt ggaatactcg
     2281 gtggatgcag acatgatctt cataaaagtg aactgatcga ctaatttgaa gatcgttacc
     2341 cctgaaccca caataataaa cggaatcgct tctttaacgg tatctaacaa caattccttc
     2401 gttgcaatat ttattttgtt ttcgctgtag tgaataaacg ccgatgtata cgcttgatgt
     2461 ttcttcaaaa aatacaaaag gacaagaata cttgctagca taccgataaa tgccgcgaaa
     2521 gttgattgcg tgaccgccgt cacatagtca ccatccatga ctttcatgat gatgaatgta
     2581 gccaatagca tataaaaaat acgtgccacc tgttctacaa tctgcgacag cgcataaggc
     2641 atcatttctt gattgccttg caaataccct cgtatcacac tcatacaagg aaaaaccaat
     2701 acagcgatac tcaaagaacg catcgttgga atcagttctt ctccgccacc agaagcatga
     2761 gctagccaag gagaggcgat gtacatgaac aaagcaaaca acaggcctaa cccgcccatc
     2821 atctgtaatg cacggataaa taatcgtcgt gaagtgccat attcattcaa tgaattatag
     2881 cgtgcagttt gttttgcaat cgcagcaggt attcctgctg tagagatcag tagaaacaat
     2941 gcatatatat tgtatcccat attgaataat ccattggcag ccttagcatt ttcgcccatc
     3001 caagcatacc agggaatgat atagacagcg cctagcaatc ttgaaatgat attactggcg
     3061 gtcatccaag cagagcctcg tgccattttt tcttgcggtg ttaattgatc taatgctggt
     3121 tttctttgat tatccatcgg tcagattcca actttctaca aaacattact atcattttaa
     3181 ttggtaaaac agaaactggc aatcgatttt atataacttt tataagaaca attgtttttt
     3241 tgaattcggt ttcgtttttt tggtaaagta aagaaatata cagcctttta tggctaacat
     3301 ttataggaga tatcaatgac acaagaaata aaaaaactac ccgttatatt tttaggtctt
     3361 ttctttatca cagttggatt gaattggttc ttgctccctc acgatatagc ttccgccgga
     3421 gtgggcgcca tcggacattt agttgaaacg gccttttcta tccaacgctc agttactgtt
     3481 tggagcatca atcttatgat gctgcttcta gccgctgtcc tattaggaaa atcagtattt
     3541 tttaagactg tagcgggaag tctgcttttt cctgttgttt tagagattgt tccaaaagtg
     3601 gaaatgatct cctcacattt cttttcactg atttcaggaa gtcttttgtt cagtcttggg
     3661 gtttacacat tgtacacagt cggggcctca aatggcggta taacgatacc gccgattatt
     3721 ttccacaaat tttttcggct tccaatgccg caaggacttt tacttaccaa tagtcttatc
     3781 gtctttttga attattatgt ttttggattg ttggagacac tattcgttct cctatctatt
     3841 acagtcagtt cattgtttat gaaaatactc atccgcttgt cccctgtgtc aagaaaattt
     3901 gaataaagct ggattttgtc atagcggaaa caaacgatat attaaaaaga gtgtgctata
     3961 ataaaccaag aaattgaata aagcgaggga tcaatcaatg agcttctctt tagaagacat
     4021 ccgtcattta ctattaaaag aaaatctatt aaaagaattt gtttcaacaa gaggctggca
     4081 tcttgatgta ccagaaaaag ctgaatttct tcagttaagt tatgattcaa gaaaagctga
     4141 tgcttctact ttgtttttct gtaaaggaat gaatttcaaa gaagaatatt tggattcagc
     4201 aatcgaacag ggtattcaat attatgtatc agaacaacca tacgaaaatt cagcagtagg
     4261 aatcatcgta acggatatcc gtaaagcaat ggcgcttctt gctatggctt tttacgacta
     4321 tccgcaaaat aaattgaaag tgatcggatt cactggaaca aaaggtaaaa caactgctgc
     4381 gtactttaca aaagcaatct tggatcacac aacaaataaa aaaacagcat tgctttctac
     4441 tatgaataca acgttagatg gcaaaacata tttcaaatcc catttgacaa cgcctgagtc
     4501 attagattta tatcgtatga tgtcagaagc agttgaaaat ggtatgactc atctagtgat
     4561 ggaagtttct tcacaagctt acaaaacaca acgcgtatac ggcttgactt tagatgtcgg
     4621 aatcttcttg aatatctcgc ctgaccatat cagtccgatc gaacatccga catttgatga
     4681 ctatttctat tgcaagcgac aattgatctt gaattcaaaa acagttgttt taaatcatga
     4741 aagtgactat tttgatttac ttagggaaac agccgatcta ttccagattc caacgatcac
     4801 ttatggtcgt tcggaagaaa gcaattatca agtgatccgt tcagataaag gtactcacgg
     4861 ttttacttta tcttctcatg aagatcgctt agctatctgc gatactgctt atgatatttt
     4921 gctggctgga ggattcaacc aagaaaatgc agccagtgcg atcattgccg cagcactaac
     4981 aggtgcctcc agtcaagatg cacaagacgg attaaaagaa gctcgtgtac caggccgaat
     5041 ggatcaattg atccaatcca atggagcgca tgtctatgtg gactatgcac ataattatct
     5101 aagcttgaaa acattgctga aatttgccaa aaatgaacat ccagatggcc gtgtaattgt
     5161 tgtattaggc agcccaggaa acaaagcaat ctcccgtcga catgattttg gaaaagtgct
     5221 atcagaaaca gctgacatcg cctttttgac ggcagatgac ccagcatttg aagatccgaa
     5281 aaaaatcgcg gaagaaatca atgaagcaat tactaatcca gatttgatcg ttcattatga
     5341 aatggatcgt cctgaagcga ttcgacgcgc tttagcagaa agcgctccgc aagattcagt
     5401 cgttattgcg ggaaaaggcg tggatcccta tcaaaaaatc aatggtgtag atgaacctta
     5461 tgaaggggat tatgcaatcg tgaagcggtt gattgaagaa taatggtatt tatatactag
     5521 tggtttccca tttctaagaa aaaaagggac tgtgattttt tgtcacagtt ccttttttct
     5581 tagaaatcat tttaactttt cactagctaa ttctgctgcc ttttctcttt cttcatttaa
     5641 taatttatta tcatagaatt tgataaatgg gaaccagata atgaatgcga tgattgcaca
     5701 aatgactgct aacacagcag ctttccaatc accaccacta ccaacaaatg ctccgatccc
     5761 gactggtgaa ggccacggca tctgtgcaag catcggtttg acgatttcca gccgaatggc
     5821 aaaataagcg agtgtagcag aagccatcgg agctaagaaa aacggaaccg ctaaataggg
     5881 attgtagacg attggcatcc cgaaaatgat tggttcattg atattgaata atcctggtac
     5941 aagtgaggct tttcctaaca ctttcagctg atcagatttc gcgaggaatg caataaagat
     6001 gatcaatcct aaagtcgcac ctgatccacc aacggttaca taagaattgt tgaattctcc
     6061 tgccaacgga taatttgcgc cttgtgcgtt agctgccata ttagctaata cgatcggggt
     6121 caagaaagaa gtgatgatat tcgctccatg gatccctacg atccacaatg catggatcaa
     6181 gaaataaatc accatgacac caagccatgt attcgtcaac tgcgtaacaa acccaaatgg
     6241 gatagcaatc attttgaaga tatccgttcc tagtgcaact aataatccat tgatgatcat
     6301 caccacaaac gcaatgacaa atgttgggat cagagcagtg aaagaacgag aaactcctgg
     6361 cggaacaaca tctggcattt ttatgaccca atttctcttg acacacatac aatacaattc
     6421 gaccgcaata atcgacataa taatagctgt aaagattcct gaagttccta aacgttcgac
     6481 aaaagagccc atcctaatac catttactac agtttctgtc tcattgatag aagcaatcaa
     6541 gctgatttta ccatcagtca tgatcaattc agggatacac ataaagaatg caaaaacaga
     6601 tagtaatgcc cccgtgagcg gattaacatt taatttttgt tcatctcgtt caattgacgt
     6661 taattcataa gcaaacacaa tggcaaagta aatagataaa atccccatcg tcattgtatt
     6721 gacgatcatg tacaagtcac taactttgaa aaaagttgca ttaaaaaatc cttctaaaaa
     6781 tgggaacggt aatggcaaaa tatttaaaac caaaaacatc gagcctacaa tcgtgaaagg
     6841 aatagaagcc atccccgctg ccatgacagc ccgaacgatt ttaagttgcg caattttccc
     6901 cataggtccc atcaggtatt tctgcaaaaa atcaaacatt agttattctc ctttcctaaa
     6961 tataacgctt tttcatattg tttaatacgg atatactgtt ggtcttggtt gagtgaaatt
     7021 ttctttaaac gatactgtat aaatgcactg gcaaaaatac cgcttacaac aagaatgaaa
     7081 acaaataact gtcctccatt gttatttgcc ataaaaggat acaagaatgt aaatcctgag
     7141 gtaaacaaca ctggaatcac taataaatta aagacactct ggaacaaaaa ataccacttt
     7201 gtccatggaa cgttcgtttg gtgttttcga taaagcttta cttgttctcc tactgcaggc
     7261 aaaatagcta aaatcagaat catcggtaaa ctacttccta aacttttagc tgatagaagc
     7321 agaatcatcc attgcatgtt catgaaaaag aaaaaggcag tcaggtaacg tacgattaga
     7381 taacgattga aatacatgtt tttcaaacta agttgctttt tttctaattc gattttcctt
     7441 tttactgtat cctgcacaat gatccttcct ttctatttat ttttttgata caaataattc
     7501 atttcctgtg caacttccaa taatgtcatc gtcgtcatca gatgatcttg tgcatggacc
     7561 atgataattt ccatttctat ttttgtcccg ctggcatatt ctttcagaag ttgagtttgt
     7621 gactgatgcg catgaagaat ctcttcattt gcttgtcgca acttctcttc tacttcttca
     7681 aacatctctt tacgcatcga gcgaaatgct tcatgtatca gtgtccgtgc atttccgcta
     7741 tggaaaatga tatcaaaaga agtcacttgt agtgtttcac tgtttatttt ttcagccatc
     7801 tgttttcctc cttattcgat cagtttgata aatttactgc tgaaattctc aaaaataggt
     7861 tcagctaaga tttcctgctg tacttttagc tggtcaacta acctaacgat tgctttagtc
     7921 acgaccgtaa ttccttcatt ttcgacataa gaaggcgaaa ctaaaaaaac aaaatgaatt
     7981 tcttgatatt gatcccactg catcccagaa ggaatcaatg caacggctac ctttgtttcg
     8041 attccaactg gaacagcggg atgtgggact gccactgtat cactgaatat gatttgtccc
     8101 atattttcac gttgctggat ctgctttcgc atttctctta tatatccttc ttcttcattt
     8161 atgctcagct tttgcagcaa atcatgaaga atttcttcct ttttcggttc gtacgtataa
     8221 gtcttgaaaa atttttcaga caactgttcg gtgtaaatct gctttttttg ctgtagagaa
     8281 aatgctggta tcggtctatc tgaggaatga tagggtatag attctccaac tacctttcgt
     8341 attttacgaa catcatcttc attcaaaaaa atactgacat gtaaaactgg aattttgaaa
     8401 aacattgtcg aaagatcaat agaagaaata atcaggtcga ttccttttaa agttgtttca
     8461 ttgatctcat aatagccttt tacacttacc actgtaatat ttttaccgaa ttcactgact
     8521 acgcgattct ttaatagttg ggcactgccg taacccgttg cacagataat caatgcatgt
     8581 agtttatgcg cagctctttc tttttccaat gccgccatga gatgcaaagc caaataggcc
     8641 cattcatctt cattgatggt atagtttttc aaagccggca tgttgctgaa atgccttttt
     8701 actgcgtcaa agactttggg attttctttc tttatctcct cagtcaatgg gttctcaatg
     8761 cgaatacctc ggctcaatcg aatcatcatc ggtgacaaat gatcgagtag tccattgaaa
     8821 agctggagat cctctactaa tgaaaaatga aattcctgag acaatctttt cagaactttc
     8881 gctaattcct ttgatagttc ctgatcattt tgattttctt tgtgattcga cttagccatg
     8941 agatgtaatg tcaaataagc aatttcttct tcgggaaacg atacacctgc gattacctca
     9001 atacgttgaa cgatccgctg cgccacttga tactctactc gcttagaaac ttcttgttcg
     9061 atgcctaatt ccttgatttc aaacccttct cggagccgtt tgatccccaa agccaagtgt
     9121 aaagtcagat tctgtatgat aaaatccgaa acttttaact tagcttctct tgtttcatcg
     9181 agaatgataa tgactagctc ttcaaaactg atgtctggga aaaaacggct ttgccctaaa
     9241 tattctttca atgaatttcc ataatggttt ccaaaaaaag tatccatgat aaaatgccgt
     9301 cgatttcttt cttccccttc gatccatgtc ccttgaccat gcttagaaac gatttttagt
     9361 gaatatggaa tcaatcgctc cttgatttct tgcatgtcct ttgtcaaact ggaacgactg
     9421 atataaagct cttctgccaa atcatccata aaaagaatgg catcttccaa aagcaattgg
     9481 ttcaatatat acttttgtcg atcttcggac tcataaaatc cttgcggttc tctagcatat
     9541 ccaggtacta tttcttgccg actcaaaaac aagtcaaatg tcagtctttg taaaatatgc
     9601 agttggtagc cataacctgg tttagcaatg atcttacctc cgtttttttc tactaactct
     9661 ttgatccgct gcaagtaatt acggattgtc cgatcagata ataacaattc agaagctaaa
     9721 cgttgactgg tgacatactc cttcttgtga tcaatcaaat atctcaacag caatttttcc
     9781 ttctgtttca ttatcatcca ctcctattcc atcatatgtt ctcttcgatc acctctgcta
     9841 acttctggat gcctgtaggg attgggacat acgcttggaa agggatacta acaacaggct
     9901 ttccaacaga ttctcctaat tttttgaatt tatccaaaaa catcgtcgtt tgcgggctga
     9961 tcagaaataa atcaaaatca ctattcttaa tcatattatc gccttctgta gctgaaactg
    10021 catctacgag tacctcttct cttttttgct taaagaaatc cgttgtcttt gaggccatca
    10081 tcgaggatga cattcctgcc gcacaaataa tcaatgcttt tttcataaat caatcactcc
    10141 ttttattttt ataaaccaag catatcttgt aagcgttttc tattcaaaat atatatttcc
    10201 gtagcattgc ggaaaaaatg tgtatcatat cttgaataat aatttctggc accgtgatat
    10261 actagtgata gaattttttc aggaggggat ttagtgagta tcacagtgaa tattttgtat
    10321 acaagtacaa atggacaagc cagaaaattt gcagaagaaa tggtcgaaag aaacattgtc
    10381 aaagcgattc gtgcagaaga aggaaacgag aaatatgact attactttcc actagaagat
    10441 ccagaatctc ttttactgat cgaccgttgg aaagatgaag aagcaatcga aaagcatcac
    10501 aaatccgaaa tgatggctca aataaacgaa ctccgtaaaa agtataaatt aaaaatgaaa
    10561 gtcgaacgtt tcactgattt accgtaaaaa gatacacatg gaatgatagc aatttatcat
    10621 ccatgtgtat cttcactttt tccttatttt ttattcgatc tcatctaatg attcaaaccg
    10681 tgtttttctc ttatttttcg gataagtatt tcagtcaccc caatatacat gattcctaaa
    10741 cataccccaa ggatattctc aaaaatgcgg atcgttactg ctcctggaac cccgaataaa
    10801 ctggcagtga ttgccaaagc accaaaagaa ttaaaaactg ttttatatct ataggtagaa
    10861 caaacaccta aagctagacc acctaatata ccgacccagt taaaaggaat aaattggctg
    10921 ataccaataa ataaaacaca tccaatcaat gagccaatga ttcggtctac tgctcgttct
    10981 ttgaaaccaa tcgacatcaa tccatagcta gatacaatag aagaaaatgc aaaccctgcc
    11041 cacatcaatc gctgaaaagg gacatactct cctgcaacga tcaaaagact aatgccaaat
    11101 gcatagatga gcatccaaat tttttcctgc gaatacatgc ctttcaaaaa aatctcttta
    11161 aacaagcttt tgtctttgtt cttttctcga tgttttctgt aaaggagaac tgaaaaccaa
    11221 caaaaaaaca aaaataataa gctacttgtt tgtgtaaaga aattcttgtt caacgaatct
    11281 ttaggtaacg aataaacaat aaagagatat gaaaagccgt acaaagaagc attccccatt
    11341 ttgggatcac tagcggttgc cattaatatg ctgcttagta aaataaaatg aatcaacatg
    11401 cccataactg accatttgat cagctggaca tacggtgcta ctaatagtga gaacatgacg
    11461 attgctaaac ctatgatcga atgggaaatc ttataaccaa agtcaacaaa acgaatactc
    11521 atcaacatac agaataaaac cacagcgtat ggtttatgct tcgattcaaa taatgttgta
    11581 attataatga taaaacaaat ggcaaacaat acgatgaata atgatcgtaa aaacaatgaa
    11641 caaatataca tgcacttttc ttttttagaa gctgcttctc gtattttttg ttttaaaata
    11701 aacggatcta gttgtagaga ctgataaaaa tccacctgtt gtcgtccttt ctttttactt
    11761 ttatttttta cagtcatcta tgacaggttg taaatactat ggaacatcat atggcgttgt
    11821 cattttcttg tcaactattt ttcctattca tgtttttata aaaaaacata tcgttttctt
    11881 gtatgattaa atcaccacaa aataaacaac tgagagaacg atatgcttta tgaatggttc
    11941 tatcaaaaaa attctgagat caatagacaa aaatttgatg attacagagg tctcttacga
    12001 aaccttacag aagaaaaaaa cattgatcgt tgctgcagtt ctcttccctg ctccatgtgt
    12061 ctgtagaaat tgtggttctg ctgtggtaga tggaaatagg aaattaatgg tggtgaaaaa
    12121 caagaagaag gaaaccattg tccactttga ataatataat catatgccaa tggttatacg
    12181 cctaaaaaag cagcgctata cctgtaaaaa ctgtcgaagc cattggaacg ctcaaagtta
    12241 ttttatccgt ccaaggcact cgatttcaaa tcatgtcaga cataaaatca cttctttact
    12301 gactgaaaaa gtgtcgttat tttttatttc gaaaagttgt taggtatctt tgacaaccgt
    12361 tattcgcaca ttgaaagagt tgagaagcta tttaccaaag tcttccaaaa gaattctgtc
    12421 ttgagtgttg agggttgtga atttcgttct cacgtttccg ttgaggataa actgagtttt
    12481 atttccgcgg atggcgagac agaaaaatta atcgagaaaa tttacctaca caaaaaattc
    12541 ctcgattaac aaactatttc cttagctgca ctaatcctaa agaaatagaa ttcttagtta
    12601 cagacatgaa cgccacctac tttcagctca ccaaacatgt tctaccaaat gcgaaattgg
    12661 tgattgatcg attacttggt ttttccacgc ctttcaagga ggcgtaacct ttattcacga
    12721 gttggttgaa gctttccgag acaaaagacc cgacttgttt ttctccttat tgacaaagag
    12781 ccattaaaac tagagaaaaa attacaactg atcgctgatt atttgtagaa agaaaatcaa
    12841 agtattaccc aataaacttg agtcattagg caaaaagcac tgcgaaactc aagaatgatt
    12901 ttcgcagtgt ttttgtatat tttttattaa ctagtattac tacaaaggtt tatttttcac
    12961 ttctcaacgc agcacaaaat tgtctttact tatttactgg ttgggttcaa ccttctctac
    13021 tccgatacta atatttgttt gtatacccct tctaaaatcg gtagtttcca ttcaattgat
    13081 gggtactagt tgattttaaa aactatgctt attcaatttt ttattttagc tcatatattt
    13141 cgtttctttc taaattttat actgatgaaa acaagtagca cacccaatat tgacaatata
    13201 aactggctaa tgatggtttc tccagttttt ggaagatgct tatttccact gcttgttgga
    13261 ttgtttcctc ttatatctga gctatcagca acttttctat tctcttttgt taaaaagatc
    13321 gccacttctt gttctttttc gatcttgaaa gttactggtg tcgcatccaa ttcatacccc
    13381 gttggcgctt gagtttctac cagctgatat gctcctggtg ctaagccatc tatcgccagt
    13441 tttccatctg ctcctgtggt tagacctgac tgcagaacgg ttccattggc atcttgtaac
    13501 tcaaaaacag ctccttgcag gacttctccg ctttggtcat cgatctttgt caacacgaca
    13561 ccgcctggtg taagtcgatt ttctttcgtc aattctacca ccgctgtctg acttctttcg
    13621 atctcaaact cgattggtgt cgcatccaat tcataccccg ttggcgcttg ggtttctacc
    13681 agctgataca ctcctggtgc taagccgtca atcgccagtt ttccatctgc tcctgtggtt
    13741 agacctgact gcagaacggt tccattggca tcttgtaact caaaaacagc tccttgcagg
    13801 acttccccgc tttggtcatc gatctttgtc aacacgacac cgcctggtgt aagtcggttt
    13861 gctttcgtca attctaccac cgctgtctga cttctttcga tctcaaactc gatcggtgtc
    13921 gcatctaatt catacccc
//
LOCUS       DAIGOK010000072        13924 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.072, whole genome shotgun sequence.
ACCESSION   DAIGOK010000072 DAIGOK010000000
VERSION     DAIGOK010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.072"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            79..2008
                     /locus_tag="NOA01_001722"
                     /pseudo
     CDS             79..2008
                     /locus_tag="NOA01_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            2134..2688
                     /locus_tag="NOA01_001723"
     CDS             2134..2688
                     /locus_tag="NOA01_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCI0632911.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQFFVLYAVEYRKMFQRK"
     gene            complement(3418..3732)
                     /locus_tag="NOA01_001724"
     CDS             complement(3418..3732)
                     /locus_tag="NOA01_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HCI0632912.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(3746..4006)
                     /locus_tag="NOA01_001725"
     CDS             complement(3746..4006)
                     /locus_tag="NOA01_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HCI0632913.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            4176..4490
                     /locus_tag="NOA01_001726"
     CDS             4176..4490
                     /locus_tag="NOA01_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCI0632914.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            4492..6147
                     /locus_tag="NOA01_001727"
     CDS             4492..6147
                     /locus_tag="NOA01_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCI0632915.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            6230..8365
                     /locus_tag="NOA01_001728"
     CDS             6230..8365
                     /locus_tag="NOA01_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCI0632916.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            8540..9049
                     /locus_tag="NOA01_001729"
     CDS             8540..9049
                     /locus_tag="NOA01_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632917.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            9046..10350
                     /locus_tag="NOA01_001730"
     CDS             9046..10350
                     /locus_tag="NOA01_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCI0632918.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            10446..11315
                     /locus_tag="NOA01_001731"
     CDS             10446..11315
                     /locus_tag="NOA01_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCI0632919.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(11427..11747)
                     /locus_tag="NOA01_001732"
     CDS             complement(11427..11747)
                     /locus_tag="NOA01_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCI0632920.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            11850..12296
                     /gene="dhaM"
                     /locus_tag="NOA01_001733"
     CDS             11850..12296
                     /gene="dhaM"
                     /locus_tag="NOA01_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HCI0632921.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            12271..13281
                     /gene="dhaK"
                     /locus_tag="NOA01_001734"
     CDS             12271..13281
                     /gene="dhaK"
                     /locus_tag="NOA01_001734"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCI0632922.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            13309..13914
                     /gene="dhaL"
                     /locus_tag="NOA01_001735"
     CDS             13309..13914
                     /gene="dhaL"
                     /locus_tag="NOA01_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HCI0632923.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
BASE COUNT         4664 a         2445 c         2956 g         3859 t
ORIGIN      
        1 gctgtgttct atccttttat ttatctctag gcccttattt attttgggac aggtatctct
       61 tcaagaaaag gagagaatat ggacaacgaa atgattagtc aattacaatt tgatcggatc
      121 aaaaaggaaa tccaagcaag agcaattgga aactatagca aaaaacgaat cagcgatatg
      181 acggtctcga caaacctaca aactgtcttg gatcgtcaag aagaaacaag agaagcacgc
      241 ttgatcttag aaagcagtca gcatgttcca tttatggggt tgccgaggat cgatgcactg
      301 acagaacaag tcaaaaaagg gttgattctg cagccaactg atttgatcga atatgcggat
      361 tttcttagaa gcagtcggat gatcacaaag ttttttgata agaatcaata ccagacaccc
      421 ttgttgtttg cttatagcaa acatcttcct gatttgataa atgtagaaga gttgattgac
      481 cagaaaataa aaaataataa agtcagtgac gacgcgtcac gtaatttgcg taaagtacgc
      541 aaacaattac aactaatcga aaaagaaatc cagtcaaaat tactgaaatt cttgcgtcat
      601 ccgaaaaaca aagaaatgat ccaagaagca atgattgttc aaaaaggcga atactatacg
      661 attcctatca aagccagcta taaaaataaa atagatggaa cgattatcga tgaatcaaat
      721 aaaagaacga cggtttttat tgaaccaact gtgatttcta aattgaatga acactatcag
      781 ttgttaaaag ctgaagaaat cagtgaagaa tatcaagtcc tggcggcatt gactggtgct
      841 atcgcagaaa acgaagaagt gatcgattta ttgatcgaaa cgatcacggt gttagatatc
      901 atctttgctc gggcaaaatt tagccgagaa atcaatggaa gcacacctag aatcaataaa
      961 tcggaacata tcattatcaa gcaaggcaga catccatttt tacctgagca tgcggtacca
     1021 ttggatgttg aaatcggtaa agactatcga ggcttgatta tcaccggagc aaatgccgga
     1081 ggcaaaacag tcgtgttgaa gacagtcggt ttattgactt tgatggcgat gttcggtatg
     1141 caagttccgg caaaagaagg gacagagctt gctgtctttg atgagatttt cgtcgatgtc
     1201 ggtgaccacc aaaatttaga aaatgcgttg agtacatttt ctggtcatat gcaaaacatc
     1261 gctgctattt tgaaaaagat aaaaaggaat acgttggtac ttttagatga gattggcagt
     1321 ggaacagagc caaatgaagg ggctgcacta gcgatcgcaa tcatggaatc tatgtatgag
     1381 caaggggcat tgatcattgc cacgacccat tatggagaaa tcaagaaatt cgccggagat
     1441 catgaagatt ttgtgccggc tgctatggct ttcgatcggg aagcacttcg tcctaaatat
     1501 cagttgcggg ttggaagaaa caggagaaag ccaagctttg tggattgctc ataaaatgga
     1561 tatgtcgatg aaattgatcc atcaagcaga gcgttacttg gcggaaaaag cctatcggac
     1621 acagaaaaaa gaatttcttc ctaagacagc agttacaaat aggaaggaga ataaaaaaga
     1681 acgtcagctc tttgcaaaag gtgatcggat atttgtcaat gaataccaaa aagaagcgtt
     1741 agtttatgaa gatataggtg aagatacgat tgatgtatat ttagacaaaa aaataatcca
     1801 cgtgcctcgt cagcgtgttc gtctggtgcg ttcagcagaa gatctgtacc cgacaggtta
     1861 cgatttagac agtctgttta tcgattataa aacacgtaaa cggcagcgtg acctagagcg
     1921 aggatcaaaa aaagctcata aagtactagt aaaagaaatg cgcaaacgac aggaagaaag
     1981 aagagtaaac gatgaaaaca gcaagtgatt atctcaaatt agctcataat atcagtgtct
     2041 gtaaagaaaa ataaaaacag tgaaatcatt ggttttattc cccgtgtaag ctaaaataaa
     2101 aagaaaagag tgctgaggag gtagggcgat aaaatgaaat taagcacaga ctggcgtaaa
     2161 gaaatacaaa cgattccgaa tcttttatcg atattccgta tttttttgct gccgatttat
     2221 ctttattttg tgctgagaca gtcattttat atagcaggag cggtcattgt tgtctcaggt
     2281 ttgtctgatt atctagacgg ggtcattgct cggagatata atcaggtaac agacttaggg
     2341 aaggtgcttg atccgtttgc agataaattg acacaacttt ttctgatttt gtctatggca
     2401 tggtatcgac cgtggctttg gctgttgttt ggtctattct tgataaaaga aggctttatg
     2461 tttgtagctg gattaatcgg gctatctaaa aatatcaaat taagtggagc gaagtggtat
     2521 ggcaaagtag cgactgctgt catctatgtt ggcatgatcc ttttgctgct tttcccagaa
     2581 ttaccaacgc tgtgggtgag agtcattttt gccgtcatca cttacggtct tttacaattt
     2641 tttgtcttgt atgctgtcga gtaccgaaag atgtttcaaa gaaaataaaa aaggggtgtg
     2701 acctatgtcg cattcctttt tttgataaat agactcccag tagctacttc tcgataataa
     2761 gccaaccgaa ccaaaaacag gagaagttgt ttttgaaatt atttcttgtg attcggagtt
     2821 aaatggtagg ctcacaacct cttggtaaac ggtgttcaat cagctgacct tcggtattag
     2881 ctcaaaaaac ggcaaaatat gagcagctat ttttccgttt gtttctctaa tactcaggtc
     2941 aaaccgctga ttttacaacc tcttccttaa acggtattca aaagccagtt tctcggtatt
     3001 aagccaaatt tatgcaaaaa tgtagaaatc cattttcaca aaatttttct taatactcga
     3061 aactaagcga ctttttcaca atctcttcct taaacggtgt tcaatcagct gaccttcggt
     3121 attagctcaa aaaacggcaa aatatgagga gctattttcc cgtttttttc tctaatactt
     3181 aggtcaaacc gctgatttca caacctcttc cttaaacggt gttcaaaagc ttgcatctgc
     3241 ggtaataagc ccaaccaaac gaaaaatatg agaagctatt tttgtttggc tgttcttatt
     3301 acttgatgca ggacagcgtt ttcacaacct ttgattcacg gttttccaga agtagcttct
     3361 tcgaaaaata agccgaaatt tcttgaagct gatcacttct gtcgcaacct ctactgacta
     3421 aggcttgata taagcataac cttgggattg cagttcaact aaatcaagga cacctgctgg
     3481 tacaacgatt acttgttcag gtaaagaact ttctgaaata tgattagcac gcatagcatt
     3541 ggcgcaagcg tgaaaaacga ctccttgcaa atctaagaat tctgcattgg cagagtctag
     3601 ataaccggta atcgcgatgc catttacaga tacgacaatc gtcgtttctg gagaagcttt
     3661 tatcaggttt tttacgtttt tccctgtttc tgaccatttt tctaattcat caatatggaa
     3721 aacgactttc attgaaatca ttcctttact ttttaataat catcttgatc gcttgttcta
     3781 gtttagccgc ttgttcttct ggattactat caggtttctc aaagcaattt ttcagattat
     3841 ctgcaacgat catgcccatt acgcgatcaa tactagaacg aacagcgctt aattgagtga
     3901 tgacatccat acattctttt tcgtcttcga tcattttttg gataccacga atttgtccct
     3961 ctgtacgttt caaacggttc aggatatttt tattatcaat ctccataaca cattctcctc
     4021 tatttttata attagataat accccttatc ggtattttat gcaaatataa aaagtagtga
     4081 aaaaaatgaa gagtcattct ggttgacata ttaataaagc tcctgtttaa tataccccat
     4141 gaggtattta taaaaatgta aatgaggtgc agaaaatgaa tttgctattt caatcaataa
     4201 aatcgataac agtcccagaa ttaaaggaaa agcttttgga aaatccagcg ttacttgatg
     4261 ttagaacacc tgcagaatac cggggcgggc atataaaagg agctaaaaat gttccattac
     4321 aaagtataaa cagatatgat ggagacaaag aaaagaccgt ctatgtgatt tgtcaatcag
     4381 ggatgaggag taaacaagca gctaaagaat taaagaaatc tggttatgat gtagtgaatg
     4441 tacgtggtgg catgaatcaa tggtttgatc gaacagtagg agggaaataa gatgaaaatc
     4501 gttattgtcg gaggtgtagc aggtggaatg tctgctgcta cacggcttcg ccgattaatg
     4561 gaagatgcag aaatcgttgt ttttgaaaaa ggaccgtatg tttcttttgc aaattgcggc
     4621 ttgccttatt atctttcggg agaaatcagt gaacgggaaa atcttcttgt ccaaacgcca
     4681 gaatcattat ccgctcgttt ttgtttagat gtgcgtccaa atcatgaagt gacagccatc
     4741 tttcccgaaa acaaaacggt agaagtcgta catgagggtc aaaaacacat tgaacagtac
     4801 gatgcattgg ttttatctcc tggtgcaaaa ccagttgttc catcgattcc agggataaca
     4861 gaagccgaca atgttttttc tattagaaat gtaccagata tcgataaagt gatgcatgca
     4921 ttagaaaaac agccaaagcg tgccgtgatc gttggtgcag gattcatcgg attggaaatg
     4981 gcagaaaacc taaaaagaag aggtttagaa gtcatggtga tcgaacaagc accacatatt
     5041 cttccaacac tggatgaaga aatggcagct tttatagaaa aagaattgtc tcatcaagga
     5101 gtagaagtga ttacttctca tgctgtcgct ggatttgaag accacgggaa acgattgcga
     5161 ctggatgatg ggcgtaccat ccctgctgat ttagttattt tatccattgg tgttcgtcct
     5221 gataaccagc tagcagtgac cgctggaatc gaattaggca tacgcggggg tatcctagta
     5281 gacgaacgtt atcaaacgaa tattcctgat atttatgcgg tgggggatgc tatcgttgta
     5341 aaacagcaaa tcactggaaa agatgcactt atttctcttg cttcaccagc caatcgtcaa
     5401 ggtagacaag ttgcggacac gatttccgga atttctcgaa gaaatcaagg cggtattgga
     5461 acagcaatta tacgaacgtt tggaatgact gccgcatcca ccggtttaag tgaaagaaca
     5521 gccaaagaaa acgaactgtc ttttgaagtc attcatgtat caggaaaaga tcatgcaagc
     5581 tattatccag aagcaacaga tattttactg aagttgatct tccatccaga gactggcgag
     5641 atttatggtg cacaaggtgt tggggcaaaa ggtgtggata aacggatcga tattttagca
     5701 acagcaatca aagggcattt gacgatcttc gatttaccgg aattagaatt gacgtatgca
     5761 ccgccatttg gctcagccaa agatccagta aacatgctag gatatgcagc aatgaacatt
     5821 gcagaagggc ttagtgaaac cgtacaatgg catgaattgc cgacagaact agcaaaagga
     5881 aaaattttat tagatgtgcg aacagcagaa gaattggaaa agggcaaatt caaggaagcc
     5941 aaacatatcc ctttgaatga acttcgggac cgattaaatg aattagacag ccaacaagaa
     6001 tatatcgtca gctgtcatag cggattacgt agctatctag cggaaagaat cttgaagcag
     6061 tctggctacc acgtaaaaaa ccttgatggt gcattttctt tatatcaaac tgtccgacct
     6121 gaagaactga tatatcctaa caaatgatac tcgttaaaaa gcttgcattc gactcttaaa
     6181 agaggtaaac taatcggttc caccacttta aaaaaagagg agaatgtgta tggtcaacac
     6241 acaaaaacag ttagaagaag catatctgaa gaacgtgtat caaaagctaa ttgataaaaa
     6301 aaacgaactg cgggaatcga tggatacggc aaaaatagaa ggaaaccagt cgttgaaaga
     6361 aatggctggc gagcttcgat tgaattttga cagttactta gataacctgg atactttttc
     6421 gatgattgaa atgaaaaata gagaaatcga tcagatgaat atcaaacaga aaaacgcaga
     6481 aaatcagcta gcaaagatcg aacgtttatt gaaaagtccc tactttggaa aagtcgttgt
     6541 agactttctg gataatgagc caaaagaatc cttttatatc ggtacagcaa atttcacaga
     6601 tgatgcagat gaaaatcttg tttatgattg gcgttcacca attgccgaac ttttttacaa
     6661 taacatgatt ggtccatctt cttatacggt tcgtcaaaat aaaatcgata cggttattaa
     6721 agaaagacga caatttcttt tggaaagaga tcgtttggtc aagttttttg atacgtctgt
     6781 agctattcaa gatgacgtat tattagaagt cttaggccaa gatgagacca atgaaatgaa
     6841 ggctatcact gctacgatcc aatcggaaca aaacacaatc attcgggatg tgaaaagtca
     6901 aaatatcttg gtaaatggcg tagcaggaag tggtaaaacc tctgctatca tgcagcgtat
     6961 tgcgtatctg ctctatttat atcgagatac gatgacatcc gatgacttgc tgattctatc
     7021 acctaatcat cggtttatcg actatatttc gaatgtccta ccatcattag gtgaaagaaa
     7081 tccactgaat ctaaccatca tacagctggt ttcacagctt tcagcagagg aaatagaagg
     7141 tgaagaggct tatttcaaac gtatcacggg agaaaatgta tcggagcaga cagaacgttt
     7201 gcgtagcaaa aagtttatcg ataatctaaa acaatcagat cctttgtttt tagatcatcc
     7261 gaattttatc cgtggactta ctaaaaatgg aaaaacagtc ctttctaaaa aaacgatcga
     7321 aaaaatatat gagaaagttc ctgctcatcc taaattgatc gataggcttc aagcaacgaa
     7381 aaaggcactg atgagcgaat ggaagaatca tcttttgaaa caagcaaaaa gtcctgctgt
     7441 gcagaatcag gtactctcgt tgacagaaga taggcagttg gaattattcg gtaaattgat
     7501 ttctgatgat tcggaacaaa gtattgtagc ttatgcaagg aaacttttac aaaaaaaata
     7561 tcgaaaaata accagacaaa tcgaagaaat ggcatgggtg gatgagcatc agttgtttga
     7621 aagaatttat gaaaaacgat atggctcagc ttatgcttgg caacccacac gtactgttga
     7681 tgaagcagtc attatattag ctattcgtca tttacttgtt gaaaaggtaa atgtaccagc
     7741 atttcgctat ctattgattg atgaagtaca agattatacc ctggcacaat taggtctatt
     7801 gatcgaatta tttccaaaaa ctcactttac tttagtaggt gacgagaatc aggcaatctt
     7861 taattcttcc actacttttg ctgatattat gcgttgtttc gacgactacc atctgccgat
     7921 ccatcggtat gatcttagaa atagttaccg ttctagcgga gcaatcacag agcttttcaa
     7981 aacctatgct gtggatcaag agaagatcga catcgtatcg atccgaccgc aaggaaaaga
     8041 acccgaatac cgctcttttc gatcctctga agaattgttg aaaatactag ctgagatttt
     8101 ggtttctcta aaaggagaaa aagcggcagt tatcacccaa acagaagagg aaacccaaac
     8161 gctacaagaa tcgattaaaa aaaattcata tggattagaa aactgccaaa tcattccttt
     8221 gagtctagca aaaggactgg aatttgatca tgtaatctta tatccatttg aaaacgatgg
     8281 agatgagcaa agaagacgca gacaaatgta cacggcgata tctagaggaa tgaaaagcat
     8341 tgtcgtattg gaaagagcaa cataattaag agataaaaaa taaaaagaac aactgataaa
     8401 aagaacaaaa tatttttcat ttgctgatga gaaatgtttt gttctttttc ttaggaaaaa
     8461 tttaggaaca ctttatgtga aaagaaatga aatgtatggt aaactcctac gtcgtaacaa
     8521 aaaaacgtga ggtgaggata tgaaaaaagt aacaagagaa gaagttgcat caattttggt
     8581 gaaagataag tatttttcaa aaggtaatta ctggttaaag atttggcaaa cgttagtggc
     8641 tcttttagga tggatgatcg ttgttcttcc ttttatatgg gtctttttcc cattaacgag
     8701 accagaaaga gccaaggatt tttccttgta cgctttttta tcagaaaaga aaatgctgta
     8761 tttcttaatc ggcttttttg tattgatttt tttcagtttc ctgattacct catttgtatt
     8821 gactaggtgg aacaatcgaa atctatacaa aaaagtaaat aaatcttttg aatatgaaga
     8881 tgaaaaattg aaaaaaagac aagaattact tgaagaagtg tatacggaac gttttggaac
     8941 aaaagaagag agagaaacgg tccgttttta ttctgtctcc gaagagaaaa atctagatac
     9001 gacattcatt gcagatttgt ataaagaaaa tggggtggaa ctgaaatgat ctcaatcacc
     9061 catacaggta atttattctt agatacttgt ttgtctatta tgtatttttt tctcgtttct
     9121 tatccgattt taggcggatt tgtatggttt attggtgtct ggtgttatgt gtttttatac
     9181 aaacataagc aaaaagaatg ggtagatgtt cccttgagtg tcgaaccgtt catcacgatc
     9241 atggtgccag cccataatga agaaattgtg atcgaatata caatcgagta tttgatgacg
     9301 aaattgaatt atcacaacta tgaggtatta gtgacagacg atggttcaac ggaccaaaca
     9361 ccagaaatat tagctagact gatgaaaaaa tacgctaatc ttcgtgtggt tcggatcgaa
     9421 aaaaataaag gaaaagccca cgcgtttaat atcggacttg cctttgcaaa agggaaattg
     9481 attttaagca atgatgcaga cacggtaccg gagccagatg cactgattag gtatgtgaat
     9541 tatttcattc gccctggtgc aagacatatt gctgcggtca ctgcaaatat ggatgtccaa
     9601 aatcgtacca aattgattgc aaaatctcaa acggttgaat tctcaagtat cgtgggcatc
     9661 attaaacgaa cacagtcagc tgttttcggt ggtctttatg cgtacagtgg agcaaatacg
     9721 atgtatcgaa aagaagcctt gatcgatgtg ggcggattca gacaagatcg agcgacagaa
     9781 gacatcagta ttgcttggga tcatcagtta aatgactggt tatctgtttt tgctcctgag
     9841 attatctttt tcatggaagt acctgtgacg ttgaagatgc tatatcgaca cagaaaacgt
     9901 tgggcaaaag gtggaacaga agtctggctg acgaatttca aaaaagtgat gctccatcct
     9961 tttaaacata ttggaagaac gatcatcttc attgatcaaa cattgagtat tgtctggtca
    10021 atattctttt gtatttccgt agttcttttt gctggattaa tcgggcatta tgtatatcaa
    10081 ggaaactacg aacagattta tattactttt acattcagtt ttgtgttcat ctgctttgaa
    10141 atggtcgcag gatttttcca gttgctagct tcgctgattg tagatgatcg acgaagaaaa
    10201 ttgaaatacc ttcttttcgc accactatat atgcttttgt tctggatagt gaacgcgata
    10261 acgatcgtga cgactttcat tccagcagtc aaaacgattt taggttatgg aagcgggaca
    10321 tggaaaagcc cggaacgtac aaaaaaataa aagatgcacc gttttttaga caaacagcct
    10381 gttgtgttta tggttcttcc aaacattttt gcttataata agaaattagt taaaaagtag
    10441 taaggatggg aaaagtgatg aaaaaaagaa tgaagccgtt aatctggcca attgctgttt
    10501 ccagtatttt cttattggga cagacagctt ttgcggaaac ttctgaacct gctcaaacag
    10561 aaccttcaac aaccataagc tcttctggtg ctgaaaacgg tgcctcggaa acagaaatga
    10621 cttccaatgt accgacagat aattcaggag aaacgatttc atcttcagaa gatacatcaa
    10681 taaatagttc tacctcttct gaccaaatcg atcatttgca agacttggaa aataaaagaa
    10741 aagaactcga agaagcatta gaagctgcaa aaaacaaatt gaaagaagac caagcagcaa
    10801 tcgatcaaga tttagctgat cttaaaaata aagtgggaaa tgaccgtgca caacttgaga
    10861 aaaagcttgc cgaagcgaaa gcaaaagcgg aagctgtcca aacagatctt gacgggaaga
    10921 tccaacaagc aaaagaattc aatcagcaat taaatcaggt tgctcaaaca gcttctggaa
    10981 agctaagtca aactcaagcg gatatcacag cggccagtca agcaatccac ggaaagatca
    11041 gcgaagcaca aacagttgct aatgattttg aagcaaaaaa agctgactta gcagctagtt
    11101 tccaacgaat aaaagatatt ttgcattcgg caggagaaaa atcagtgacc tctggaagca
    11161 cgacagaaac agttcagcaa acgacagagc catccaaatc aacaagtcag actttgccaa
    11221 aaacaaatga aaaaacagct tctgtcagtt attccttagc aggggcgggt gtgttagtcg
    11281 cagcagctta cggaatcatc cgcaaaaaga aataaaaaag agaggatggg aaggtagagt
    11341 acctcgaagt tagactgaaa aatctaattc ttgagtgcca ctacatgtcc catctctctt
    11401 tagttattaa aacagatgtc ccctacttat ctcgtggaga cagctgttcc actacatgtg
    11461 accatcagca tgccattgtc gctgcctaat acttcatagt ccatttttac gccaatgatc
    11521 gcatcagctc ctaatgcttt tgcacgttcg gccatttcgg caagtgcttc ttctcttgca
    11581 cccattagtt cgttttcata gctttttgag cggccgccaa agatatttcg caaccccgcc
    11641 tccaagtctt taaatgcatg gatcccagta atgacctcac caaaaacgat ccctttataa
    11701 gctactacgg cttttccctc tacactgttc gttgttgttg taatcattcg atcactcctc
    11761 tcttttaatt aaagtataac aaacttcaag aagcactaca catattttcc caaaattaga
    11821 aatttattat atacttgaat tgttcattaa tgaaagcgaa accaatcgtt agcaactatg
    11881 taaaagaaag gaagattctt gtgaaaaaaa gtattttgct tatttctcat agtcaaaaag
    11941 taacagatgg tattaaagaa atgattgagc agatgcaaaa atccgaagag gtgcaaattt
    12001 tttctctcgg cggaataaat ggcgagatcg gatcagaccc gatgaaaatc gtagaagcag
    12061 tcaatgaatc aagcgatgca gctagttatt ttgtttttgc ggatatcggt agcgcggtat
    12121 tgaactcgga actggcaaaa gacatgttgg aagaagagca gcagcaacgt tatcatctgg
    12181 tggatgcgcc tttagtggaa ggagcttttg cagcagccat cactgccggt ataacagatg
    12241 atactcagca aatcttaaca gaagcacaaa atgccggaaa gaaaggatgg gaataaattg
    12301 aaaaaaatca tgaatgatcc aagcaatatt gtcgaagaaa tgctggaagg attagtaaaa
    12361 agctatccag aacttgttca tcgtgtagaa tcttcccgtg ttgtagccaa gaatcagaaa
    12421 gcggaacaag ttggtttggt ttctggtgga ggaagcggac atgaaccatc ccatgccggg
    12481 tttgtagggg aagggatgtt aagtgcagcg gtgcttggtg atgtgtttac ttctccaact
    12541 ccagatcaga ttcagacggc tattaaggaa gccgatagtg gaaaaggagt tttgctgatc
    12601 gttaagaatt atacagggga tgcattgaac ttcgatatgg caaaggaatt agctgctatg
    12661 gacgatattg aagtagaaag tgtgatcgta gatgatgata tcgccgttga aaatagtacg
    12721 tatactgctg gaaaacgcgg agttgcaggt actgtccttg tacacaaaat cgtgggcgat
    12781 gcagcaagaa acggtgcttc cctagctgag ttgaaagaac tgggagaaaa ggtcgtacag
    12841 gcaactaaga cgatcggtct agcgcttcga gcagcaactg taccagaagt agggaaacca
    12901 ggattcgaac taggagaaga tgagatcgag tatggtgttg gtatccacgg tgagccaggc
    12961 tatcgtcgag aaaagatgca gccttctaaa gtactggcaa aggaacttgt tactaagatt
    13021 ttagatgatt actcgaattt accaaaagaa gcaggtgtac tagtcaacgg aatgggcggg
    13081 acgcctttga tggaacagtt cgtgttcatg aatgatgttt tagctctgtt ggaagaaaga
    13141 ggcgtcaatg tagttttccg aaaagtcggt aattttatga cctcactaga tatgcaaggt
    13201 ctttctctaa cactgatcga cttgacggag acacaatgga aagattcttt agaaagcaat
    13261 gtacagacga tttcatggta aaaaatctca agataaaagg gtgataatat gaaactaacc
    13321 gttcaagaaa tccaagaatg gctcacgcaa tttgcagagg caatcaatca gaacaaacaa
    13381 tatctcagtg atctagatac cccgattggc gatggagacc atggaaataa catgggaaga
    13441 ggggtttcag cttatgaaga agcttttcaa gccgatcatc ctgaaacaat cagcgataca
    13501 ttcaaagtat tctctatggc gatgatatcg aaagtcggtg gggcttctgg tccgttatac
    13561 ggatctgcat tcatgaacat gatgaaagcg acaaaagagg tcgattctat tgattctcaa
    13621 gaaaaattag gcgaagtgat cgagcaggga acagcgggta ttcaaagccg aggaaaggca
    13681 gaagctgacg ataaaacgat gttagatgtt tggctgccag tcacagaggc attgaaggca
    13741 gggaatttaa caaaagaaat cattgaccag gcaaaagaac acaccaaagt cttggtagct
    13801 aaaaaaggac gagcttccta tctaggagag cgtgcaatcg gacatatcga cccaggagct
    13861 gcttctagtg ccattttatt ttcaacgctt ttagatgtga tcaatcaacc ttaagttcaa
    13921 taaa
//
LOCUS       DAIGOK010000073        13693 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.073, whole genome shotgun sequence.
ACCESSION   DAIGOK010000073 DAIGOK010000000
VERSION     DAIGOK010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.073"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(<1..902)
                     /locus_tag="NOA01_001736"
     CDS             complement(<1..902)
                     /locus_tag="NOA01_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCI0632924.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(926..970)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1185..2153
                     /locus_tag="NOA01_001737"
     CDS             1185..2153
                     /locus_tag="NOA01_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCI0632925.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2266..2544
                     /locus_tag="NOA01_001738"
     CDS             2266..2544
                     /locus_tag="NOA01_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCI0632926.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2662..2898
                     /gene="secG"
                     /locus_tag="NOA01_001739"
     CDS             2662..2898
                     /gene="secG"
                     /locus_tag="NOA01_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCI0632927.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3001..3756
                     /locus_tag="NOA01_001740"
     CDS             3001..3756
                     /locus_tag="NOA01_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCI0632928.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3759..6116
                     /gene="rnr"
                     /locus_tag="NOA01_001741"
     CDS             3759..6116
                     /gene="rnr"
                     /locus_tag="NOA01_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCI0632929.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKFGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6138..6602
                     /gene="smpB"
                     /locus_tag="NOA01_001742"
     CDS             6138..6602
                     /gene="smpB"
                     /locus_tag="NOA01_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCI0632930.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6904..7623
                     /gene="atpB"
                     /locus_tag="NOA01_001743"
     CDS             6904..7623
                     /gene="atpB"
                     /locus_tag="NOA01_001743"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCI0632931.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7676..7891
                     /gene="atpE"
                     /locus_tag="NOA01_001744"
     CDS             7676..7891
                     /gene="atpE"
                     /locus_tag="NOA01_001744"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HCI0632932.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7982..8506
                     /gene="atpF"
                     /locus_tag="NOA01_001745"
     CDS             7982..8506
                     /gene="atpF"
                     /locus_tag="NOA01_001745"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCI0632933.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8493..9035
                     /locus_tag="NOA01_001746"
     CDS             8493..9035
                     /locus_tag="NOA01_001746"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCI0632934.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9058..10614
                     /locus_tag="NOA01_001747"
     CDS             9058..10614
                     /locus_tag="NOA01_001747"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCI0632935.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            10628..11530
                     /locus_tag="NOA01_001748"
     CDS             10628..11530
                     /locus_tag="NOA01_001748"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCI0632936.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11553..12959
                     /gene="atpD"
                     /locus_tag="NOA01_001749"
     CDS             11553..12959
                     /gene="atpD"
                     /locus_tag="NOA01_001749"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCI0632937.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12973..13395
                     /locus_tag="NOA01_001750"
     CDS             12973..13395
                     /locus_tag="NOA01_001750"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCI0632938.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4526 a         2449 c         2986 g         3732 t
ORIGIN      
        1 ctttcggaaa aaagacgagt atctactttt gtcataattt tactgttagc aggtttctgc
       61 caaaattcat aacgatcaac caatgtttgt ccacgactta ttccctgagt ttccacagcg
      121 gtgaagcttt tgaaaccttc acagatggcc ccctcgatga aataagcaac agctaacgga
      181 tcattgatga cacatcctaa aattctttct tgtttccagt ggaagtcaaa ataaaaacga
      241 gtgatcgctt ttaaatactc tccctcttca ggattcattt gacagcagta ttcgaggata
      301 gtcggtgtca agacgatttc tcttgtcaca tctaacccaa ccatttctat cgtttgtttg
      361 agattctcga atacataaga agctgcttca ggatcacacc agtaattgta ttcagcaact
      421 ggcgaacagt taccatggct tttgtaagtt ccacccatcg agacaaaacg atccatatgt
      481 ttcccaatat ttgggttgac tttcaaagca aaagcgatat tggtcaaagg acccaaagca
      541 atgactgatg tatttgtctt ttctttgaaa taatcagcca aaaaatcaac ggcatggaga
      601 ggttctgcct gttttctcaa gatcatcggg aaatttgttt cccctaaacc atccatacca
      661 tgagtgtctt gtgcactgat gaaaggttgc ttcaaaggtt tttccattcc ttgataaaca
      721 ggaatgtcta aacgatcaca gcgttccagg catttcagca cattctgcgt acctatatct
      781 gcagggacat tcccacaaac tacggtaata gcaactactt cgatatcagg ggatttgaca
      841 gctaatgata aagctaaagt gtcgtcgatc cctggatcgc aatcaataat tacttttcgc
      901 attatttcca atctcctttc tttagagata cttagttttt tattctggga agtttccgaa
      961 ccagtccaag acgagcgtct tgattataat gcacgaaatt agtatactgg cttttattgc
     1021 gaatgtaaat gctctcttac aagtaaatct attgaggaga actttaagta aatgatacaa
     1081 tgaaaaagaa atggaattta cgtgtgccga aaataaagaa tcctatggat cggtacattg
     1141 gataatagat agattatagt ttggcagaaa gcggcgggtg tacaatggtt attttttatt
     1201 tcattcttat tctgataatt atagggatca gttaccattt tctttcttat ttttatgcaa
     1261 agaaattagt aagagcggga gtacagagag gatatgactg gtatgaagac actggtcatc
     1321 gtttgatgga ccctgctgca gtcacgtcag tagaaaaaaa acgacaaaaa ttagaagaag
     1381 agtgtgaaag cttttgggag cagggaacac ccaaaacaat caaaagttat gacgggttga
     1441 aactagctgg tcagatgttc atccatccaa ataagcagaa taaatgggtc atttgcgtac
     1501 atgattaccg aagcacaggg aaaagagaca tgtctcatat aggtaaaaga tatgcagaga
     1561 aaggattcaa cgttcttgta cctgatttac gtgcgcacgg agaaagtgaa ggagaaatca
     1621 tcggaatggg atggctagat cggctagatt taattgcctg gatccaactc atcttagatg
     1681 aacagccgaa tgcgtccatt attttacacg gcggatcaat gggagcttct acgatcatga
     1741 tggcaagtgg tgaaaaactt ccatcagcgg tcaaaggatt tattttagac agcggatatg
     1801 tttctgttta tgcagaattc cgatatatgt taagtaaaat cactgtcttt cctaaaaaaa
     1861 tggtcatgag atatgcgaat cattatgccc agaaatacgc cggttactcc ttaaagcaag
     1921 catcagctac gagacagctt ggcagcaacc atctgccttt attagtcatc catggtgaaa
     1981 gagatcattt tgtccctaca gaagcagcgt acacgattca aaatgctacg gcaggggata
     2041 aagcactgct gcttgttcca gaagcagaac acttggaagc atcgatgaaa gatccgaaaa
     2101 cctattggac agtgattttt tcatttattg aacaaagaat aacgatggat taattttctc
     2161 ttcttttttg taagtaattc gaggataaat aattgtttat agatctggag actgctaaaa
     2221 taaggtagga ataaaaaaat atcagttatt tgaggagtgc ggcatatgga aatcaaagaa
     2281 gaaaaaaatc gtcttgcttt attcaatgat gagcaacaag agattggaga aatgacatgg
     2341 tcagatgcgg gcccagacat catgatcatt gaccatacat ttgtagatcc agcatatcgt
     2401 ggtcaaaagt tagctgaaaa gctagtgtat actggagtag aattagcaag acgagacggg
     2461 aaaaagatca ttcctttatg cccttatgca aaaaaagagt ttgaaaagaa accagaatat
     2521 caagacgtct tacgtcagtc ataagaataa cagctgaaag ttttgaactt tcagctgtta
     2581 ttccatttat atagagttgt cacggacttt tatctatgat aaagttaaag agacgatatc
     2641 ttttgggaag gaggctgcga tatgtataat ttaattttgg gaatcgtgat cgttttatcg
     2701 attgttatgg tcattgcgat tatgatgcag ccaagcaagc aaaatagtgc agcgagtgca
     2761 tttaccggcg gtgcggatca actattcggc aaacaaaaag ctcgcggctt tgaagctgtc
     2821 atgcagcgat caactgcagt tatgggagct atctggatga tcttattatt tgtcctagca
     2881 tatttatctt cgaagtagtt gaataagcct ctcgcaaaac ggcgggcggc tttttttatt
     2941 gcaaagagaa cgcttaagaa gaagttatga aagaaactat ggtaaaatga ggcgaaagag
     3001 gtgagacgag tgagacaatt accaaaaccc ctttatgcaa aacatggaaa gcgagcggtt
     3061 ttgctgcttc acgcttactc aggaagtcca aacgatgttc gaatgctcgc acgatttttg
     3121 gaaaaagcag attatacagt ttatgcgccg ctttttaaag gacacggaac aatggagcca
     3181 tatgatatct tgcaagaaaa agcagaaagc tggtgggcag acactaaaaa agccatccat
     3241 tttttacagt ctgagcagtt ttctgatatt gccgtattag gattatcaat gggcggcatc
     3301 tttgctgtac gagcactaga agaagaatca gtgattggcg gcggtttttt ctgttcgcct
     3361 ttgtctcctg taaaaacaaa cgtgcctgaa aactttgaaa aatacgtacg tcaagtgctg
     3421 aagacagcag gtaaatcaga gaaagaaatc aatgaaaaag cagttgctta ccgctcgtta
     3481 gctgaacaac aattaatgga tatacaagat caggcagcaa tcgtagaaag taggttgtca
     3541 gatatccaac agccgatatt tttagctcaa gctgggaaag atgaaatgat cgaccctaat
     3601 ggcgtatttg aaactgcacg caagttgagc cgtcagcgag tgacacttca atggtatcct
     3661 gaaagtggtc atgtgattac agttggtaca gcaagacgcg aattagaaaa agacgtcttg
     3721 gaatttttag aaaaattacc ttggaatgag gaataaatat gacaaaagaa acgataaaag
     3781 agaaaattct ctttttcatg gaaagcagtc gcaaacgcag tttttctatg gaagaaatcg
     3841 ctgaaggttt agggttccaa aaaagcgatg attttaaatt actagtacaa acgattgctc
     3901 aaatggaaag agaacagagt atagttttca ataaaaaagg aaaagtgaaa cttcctttaa
     3961 aaccaatctt gatcgaagga acatttcgag caaatgaacg aggatttgga tttgtaacga
     4021 ttgatcccga agaagatgac atctatatcc caaaagaagc gactggtttt gcaatggatg
     4081 gagacaccgt agcaatcgat atcatgaagc gagcaaatac agatatggat cgcggagcag
     4141 aggggaaagt agtcgagatt cgagaccggg cgactactca gttagcaggg gaattcgttg
     4201 cttattcaga agaagaaata caagaaacag atctttacgg cgttgttatc ccgaaagata
     4261 gaaagctcaa ccaatttaaa gtatatgcag cagcagaggg agttcgtcca gttgacggaa
     4321 gtattgtgct gatcgaactg acccattacc ctgaaaaagg gtatgcgact agtttagaag
     4381 ggataatcaa gcaagtgatt ggtcataaga atgaccctgg catggatatc ctctcaattg
     4441 tcgtggctaa tggtatccct acgaaatttc ctgatgatgt attgtctgaa gcagatgaag
     4501 ttccagatca aatcagcgaa aaggatataa ctggacgtag agatttaaga aatcagctca
     4561 ttgttacgat tgatggagaa gatgcaaaag atctagatga tgcagttacg gttaaaaaat
     4621 tagataacgg taattatttt cttggagtac acattgcaga tgtttcttac tatgtgacac
     4681 aaggcagtca attggacaga gaagcatatg aaagagggac aagtgtatat ttgactgatc
     4741 gagtgattcc gatgatcccg caacgtttat cgaatggtat ctgttcactg aatccccatg
     4801 ttccaaggct gaccatgagt tgcgaaatgg agattgatcc aaatggacaa gtagtctctc
     4861 atgatatttt cccaagtgtg atccaaacaa cagaacggat gacctacaca gctgtcaatc
     4921 aaatcttaga agatcaagac gaagaaacga tgcaacgcta tgctcagctc gtacctatgt
     4981 tccaagaaat gggtgaattg catcgaatcc ttgaaacgat gcgaatcaaa cgtggcgcga
     5041 tttcttttga ggatcgcgaa gcgaaaatcc ttgttgatgc tgaaggacac ccaaatgaca
     5101 tccagctaag aagcagagga attggtgaac gcttgatcga atctttcatg cttgcggcca
     5161 atgaaacggt agctgaacat tataataaat taaatctgcc gtttatttac cgaatccatg
     5221 aacagccaaa agaagaaaaa atgcagcgct tctttgattt tgcttcagct ttgggcattc
     5281 tggtccgagg aacgaaagga acgatcacac caaaagattt acagcatgtg atcgaaggag
     5341 tagaagacaa accagaagct gcagttatca atactatgtt gctaagaagc atgcaacaag
     5401 cacgctattc agaagacaat tttgggcatt atggcttagc ggcagattac tacacacact
     5461 ttacatctcc tattcgacgc tatcccgatc taatcgtcca tcgtctgatc cgttcttaca
     5521 gtgaagataa aagtgaaagc aatcaaagtt cgtgggctgc ggaacttcct gaaattgcag
     5581 atcatagttc taagatggaa cgtcgtgcag tagaaacaga acgtgaagta gattccatga
     5641 agaaagcaga attcatgatg gacaaagtgg gagaagaatt cgatgggatc atcagctctg
     5701 ttgtgaaatt cggcctgttt atcgaactgc cgaatacagt agaaggattg atccatatca
     5761 atgaattgaa acaggactac ttccattttg ttgaaaatca tttagctctt gtaggtgaaa
     5821 gaactggtct gacttttaaa atcggacaaa aagtgcgcgt aaaagtagtc aaagctgatc
     5881 cagatgaacg agcagtggat tttgaactcg tttccgcaga agaggtcact gcgttggaac
     5941 gtccaaaagc acgcaatgga aaatttggaa aacgagaagg aaaacaagca ggcaaacgct
     6001 cagaaaaaaa tggcaagaag aacaaacgat atagtgatag taagcaaacg ttcggtaaga
     6061 aaaaagggaa aaagccattt tacaaagatg tcaaaaagaa gaaaaagaaa aaataaaatg
     6121 agttggagga aacagctatg ccaaaaggcg agggaaaact aattgcacaa aacaagaaag
     6181 ctcgccatga ttattcgatc atcgacacga tggaagcagg gatggtcttg caaggaacgg
     6241 agatcaagtc gatacgaaac agccggatca atctgaaaga tggatttatt cgcgtccgca
     6301 acggggaagc tttcttgcat aatgttcata tcagtcctta tgaacaagga aatattttta
     6361 atcatgatcc gttgcgcacg agaaagttat tattacacaa gaaacaaatc gttcgtcttg
     6421 aaaacgaatt gaaaaacact ggaatcactg ttgttccttt aaaagtctat attcgtaacg
     6481 gctatgccaa ggtattgatt ggtctggcaa aagggaaaaa atcgtatgat aaacgggaag
     6541 atttgaaacg aaaagatatc gatcgacaaa ttgatcgaac attaaaaaat ttctctagat
     6601 aatttcatta tcctttttcg catattcggt gcttttgctt ttacttaatg ataaaatttg
     6661 ttagcattta atctgataaa acatgattat cacaggtttt ttcttagatc aggagcaccg
     6721 tgcatgggga aaaattcgct tcgtttttga ctgaaataaa aaagtgtcta attttcatga
     6781 aaatgttttg aattttctgt ttcatggtgg tatgataccg tgtggttaga aaccgattgg
     6841 aaaaaaccag atacgcacat gaattgattt tatgtgaatt tcggggaaaa gaggtgaaat
     6901 ggattggatg aacgctcgct aacgtttcac attggaccaa tttggtttga tggtacagtt
     6961 tgtataatgg tgttactgac ctgtttgatt gttttcttct tagcttactt ttttacaaga
     7021 aatcttaagt tgaaaccaac aggcaaacaa aatgcgatag aatgggtcat tgattttagt
     7081 cgaggaatcg taacagataa tctgcctaga aaagaattga gcaatttcca tttgttggca
     7141 tttacattgt ttttatttgt ttttgttgca aacaacatag gtttagtaac aaaaatcgtc
     7201 ttacctaacg aaacaacact ttggaaaagc ccgacagcag acccgtttgt tactttaact
     7261 ttggcattta taatgatctt acttactcac ttattcgggg tgaaaaagtt aggcttcaaa
     7321 ggatatttcg taaattcgtt cttgaaaccc tatagtttta tgtttccgat gaaactgatt
     7381 gaagaattta caaatttatt aacacttgct ttacgtcttt atgggaatat ttatgcgggg
     7441 gaagttttgc taaccttgat tgcaaaaatg atgacaagct taggctggtt ctcattacca
     7501 ttagcaattc cattagagat ggtctggatc gcgttctcct tgtttattgg gagcatccaa
     7561 gcatttgtct ttgtcacttt gtcaatggtt tatatgagcc ataagattga agtagaagaa
     7621 taatactcgg ttaaaaaaca aactactatt tatctatttt aggaggaaaa caattatgaa
     7681 ttatatcgca gcagcaatcg caatcatggg agcagcaatc ggagccggtt atggtaacgg
     7741 tcaagttatc tctaaaacaa tcgaatcaat ggctcgccaa cctgaaatgt ctggtcaatt
     7801 ccgtacaaca atgtttatcg gggtagctct agttgaagct gttcctatct taggggtagt
     7861 tatcgccttg attctagttt ttgccgttta attgaatcag tattaaggca gaaacaacat
     7921 cgtttgttac tgctttttta catggaaact agaaatactc caaagaaagg aaggctcgcc
     7981 tatgctgaat caattggcaa ttgcagaagt tggcaatgcg atgttaggta atatcatcgt
     8041 tgtcagcggc tcatttttga tactactggc tctcttgaag cactttgctt ggggaccaat
     8101 cagcgatatt ttgaaaaaac gtgaagacaa gatcgccaat gatttagatt ctgcagaaca
     8161 atctcgcatc aactcagcga aaatggaaca agaacgcgaa caacaattgt tagcctctcg
     8221 ttctgatgca gctgatatca tcaaaaatgc gaaagaaagt ggagaattaa gccgccaaaa
     8281 tattttgaag gatgctcaag aagaagcagc tcgtctaaaa agcaaagccc aagctgatat
     8341 cactgtagaa cgtgattcag cgctgaactc tgtaaaagac gacgttgcag aactctctct
     8401 tcaaatcgcg gaaaaaatct tgaacaaaga attatctcca gaaatgcatg aatcattaat
     8461 caatcaatac attgaaggtc taggttcttc aaatgaaact agataaatat acagtaggaa
     8521 gacgttacgg gaaagcttta tttgaactgg cgatcgattc aaatcaagca gaagaaatct
     8581 atcaagatct cgtaagttta cgtcaaatct atgaacaagt acctggatta gggaatatgt
     8641 taagtgatgt tcgtctagaa cctcatgaaa aacgagaaat catggacaag cttgtcagcg
     8701 gttatgatgg gatcgttaaa aactttttag aagttgttta tgagtataac cgaatggatg
     8761 accttttgtt catgatcgaa gaatatgaac gtcgatatga tgaacatcaa gggttactat
     8821 tgggaagtgt cacaacagca gtcccacttt cagacgaaca acgaatacaa ttagaaaaga
     8881 atgttgcaaa aacaatggac tatcaatcag ttgaattaaa acaaatcgtt gattcttcta
     8941 ttattggtgg cgcgattgtt gaagccaacc atcgagtaat cgacggaagc attcgcacgc
     9001 aattagaaaa aatgcgtaat caattaaaca gatagtacaa agaggtaggt gaatcgaatg
     9061 gccatcaaag cagaagaaat tagtgccttg attaaagaac aaattgaaaa ttatcaaaac
     9121 gtgctttcag tcgaagaaat cggtaccgta acttatgtag gtgacgggat cgctcgtgca
     9181 catggtttag aaaacgcgat gagtggtgag ttgcttgaat tttcaaacgg ctcatacgga
     9241 atggcgcaaa acttagagtc aaatgatgta ggtatcatta tccttggcga tttcgagtcc
     9301 attcgtgaag gagataaagt aaaacgaaca ggtaagatca tggaagttcc agttggagag
     9361 gccttgatcg gtcgggtagt caatccgcta ggtcaaccaa tcgacggact aggtgaaatc
     9421 gttactgaca aagctcgtcc agtagaagcg atggcaccag gtgttatgca acgtaaatct
     9481 gttaacgaac caatgcaaac agggctaaaa gcgatcgacg cccttgtgcc aatcggacgc
     9541 ggacaacgtg aattagtcat cggtgaccgt aaaacaggga aaacttctat tgcgatcgat
     9601 acgatcatca accaaaaagg tcaagatatg atctgtatct atgtagcaat cggacaaaaa
     9661 gattctacag ttcgtacaca agttgaaaca ttgaaaaaat atggcgcaat ggattacaca
     9721 atcgttgtga atgccggtgc gtctcaacca gcaccattgc tttatatcgc accatatgct
     9781 ggtactgcaa tgggtgaaga attcatgtac aacggtaaac atgtattgat catttttgat
     9841 gatctttcaa aacaagctgt tgcttatcgt gaactttcct tgctgcttcg ccgtccacca
     9901 ggtcgtgaag cttatccagg ggatgttttc tacttacact cacgcttatt ggaacgtgca
     9961 gcaaaattaa gtgatgattt aggtggcggt tcaatgactg ccttaccatt tgttgaaaca
    10021 caagctggag atatctctgc ttatatccca acaaacgtta tttcgatcac tgatggacaa
    10081 atcttcttag aaagcgactt gttctacgca ggtactcgtc cagcggttga tgccggacta
    10141 tctgtatctc gtgtaggggg ttctgcgcaa atcaaagcaa tgaaaaaagt tgctgggact
    10201 ttacgtctag acttagcaag ttaccgtgaa ttagaagcat ttacacaatt cggttctgac
    10261 ttggatgcag caacacaagc aaaactaaat cgtggacgcc gtacagtaga aatcttgaaa
    10321 caaaaattgc atgcaccgct agctgttgaa aaacaagtag tgattttgta tgctttgaca
    10381 catggattct tagatagtat ccctgtagac agtattttag acttcgaaca tgaattattc
    10441 gaatatctgg ataccaatca ttcagatatc tttgaaacaa tccgtacaac aaaagatctt
    10501 ccagaagaag aagcgttgaa cagtgcgatt caggaatata aagacatgtt cttagctaca
    10561 aaaggaaata attcctctac agaagataaa ttgaaatcca ttcaaaatgc atagtgaggt
    10621 gagatgaatg ggtgcttcat taaatgaaat caagacacgt atcgcttcaa cgaaaaaaac
    10681 gagccaaatc acgcgggcaa tgcagatggt gtctgcttct aagttgacaa aatctgaagc
    10741 ttcttcacag aagtttcagg tttatgcaac aaaagtaaga gaaatcgtta cgcatttgac
    10801 tgctactcag ttaaatgata ttgcctcaga aaatcctaga ggggatatca attacaacag
    10861 catgttgctc agccgtcctg ttaagaaaac gggttatatc gtcatcactg cagatggcgg
    10921 tttagtcggt ggatataaca gctcgatatt gaaacaaacg atgtctattt tagaagaaga
    10981 ccaccaatca cccgatgatt acgtgatgat cgcaattggc ggaactggtg cagacttctt
    11041 taaagcacgc ggtatcaatc tagcatatga gctgcgcaat ctatctgatc agccaagctt
    11101 tgatgaagtc cgcaaaatcg taaatatggc aacaactatg taccaaaacg aagtattcga
    11161 tgaattatac gtatgttata accatcatat caattcgcta actagtcagt ttcgtgtaga
    11221 aaaaatgctg ccaatctcag acttagatcc agaagaagca actactttcg aacaagaata
    11281 tatttttgaa ccttcaaaag aagaaatctt gtctcagtta ttgcctcagt atgctgaaag
    11341 tttgatctac ggtgcgatcg ttgacgccaa aacagcagaa catgctgctg gtatgacagc
    11401 aatgaaaaca gcaactgata atgctgctaa tatcattgat gagctgacgg tttcttacaa
    11461 ccgtgcaaga caaggggcta ttacccaaga aattaccgaa attgttgccg gagcttcggc
    11521 actagaatag tattgagatt ggaggaaaaa agatgagttc aggcaagatt gttcaagtaa
    11581 tcggtcccgt tgtcgacgtg gaattttctt tagaccaatc cttaccagat atcaacaacg
    11641 cattagttgt ttataaaaat gacgaaaata aatcaaaagt tgttcttgaa gcagcgttag
    11701 aattagggga cggagtgatc cggaccattg cgatggaatc aacagatggt ttacaaagag
    11761 gaatggaagt cattgataca ggcaaagcaa tctctgttcc tgtaggtaaa gaaacattag
    11821 gtcgcgtatt caacgtacta ggagatacga tcgatttaga aacacctttc ccagaagatg
    11881 cggaaagaag cgaaattcat aaaaaagcac cagcctttga cgaattaagt acaagtacag
    11941 aaattttgga aacagggatc aaagttatcg atttgcttgc cccatattta aaaggtggga
    12001 aagtcggact attcggtggt gccggtgttg gtaaaaccgt actgatccaa gaactgatcc
    12061 ataatatcgc ccaagaacat ggtggtattt ctgtatttac cggtgtaggt gaacgtactc
    12121 gtgaaggtaa tgacttgtat tatgaaatga aagattcagg agttatcgaa aaaacagcca
    12181 tggtgttcgg acaaatgaac gaaccaccag gtgcacgtat gcgtgttgct ttgactggtt
    12241 tgacgattgc ggaatatttc cgtgatgtag aaggtcaaga tgtactgttg tttatcgaca
    12301 acattttccg tttcactcaa gctggatctg aagtatcagc cttgttagga cggatgcctt
    12361 ctgcggttgg ttatcaacca acattggcaa cagaaatggg tcaattgcaa gaacgtatca
    12421 catctacgaa aaaaggttcg atcacatcga tccaagcaat ctatgtgcca gccgatgact
    12481 acactgaccc ggcgccggca acagcattcg cccatttgga tgcgacaacc aacttggaac
    12541 gtaaattaac agaacaaggg atttatccag cggtagatcc attggcttct tcttcaagtg
    12601 ctttagcacc tgaaatcgtg ggagaagaac attataaagt agcaacagaa gtccaacacg
    12661 tattgcaacg atatcgtgaa ttacaagata tcattgctat cttaggtatg gatgaattat
    12721 ccgatcaaga aaaagtgtta gtttctcgtg cccgcagagt ccaattcttc ttgtcacaaa
    12781 actttaacgt ggctgaacaa tttactggtc ttccaggttc atatgtccct gtagaagaaa
    12841 cagtgaaagg attccgcgaa atcttggaag gcaaatacga tgatcttcct gaagaagctt
    12901 tccgaagtgt tggccgaatc gaagacgtcg tagaaaaagc gaaaacacta ggctactaga
    12961 aaggggtgcc ccatggatca atatttaaca gtaaatgtgg taactcctgc cggtttggtc
    13021 tatgatcacc atgcagctat cgtcgttgca cgtacgacag ctggtgagct tgggattttg
    13081 cctaaacatg caccaatcat cgttccattg acgattgatg aagttcgtgt aaaacgaaca
    13141 gattcagata cccacgtcga ttggatcgct gttaatggcg gaatcatgga agttcgtgat
    13201 aatgttgttt caatcgttgc tgatagtgcg gaacgtgaac gtgatatcga tgtatctcgt
    13261 gcggaacgtg caaaacaacg cgcagaacgc caaatcgaag aagcaaaagc aaaacaagat
    13321 acgaacgaat tgaaacgggc aactgttgcg ttgcaccgag caatcaatcg tatcaaagta
    13381 tctaagcact cataaaatga gatgcggatg tttatccgtg tttaagaaca gtaaaaagag
    13441 gttatgacag aagtggtcag cttcaagaca taagaaggaa tttacgaaaa ttgcttctca
    13501 aatttttgtg aaatctcagc ttatttttcg aagaagctac ttctggaaca ccgtatatcg
    13561 gaggttgtga aatcagcgtt ttgtcctgag tattagagaa acaaatggaa aatagttcct
    13621 catattttgc cgttttttga gctaataccg agggtcagat gattgaacac cgtttatttg
    13681 gaataaggga ctg
//
LOCUS       DAIGOK010000074        13667 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.074, whole genome shotgun sequence.
ACCESSION   DAIGOK010000074 DAIGOK010000000
VERSION     DAIGOK010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13667
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.074"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(3..434)
                     /locus_tag="NOA01_001751"
     CDS             complement(3..434)
                     /locus_tag="NOA01_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HCI0632939.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            513..926
                     /locus_tag="NOA01_001752"
     CDS             513..926
                     /locus_tag="NOA01_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HCI0632940.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(964..2112)
                     /locus_tag="NOA01_001753"
     CDS             complement(964..2112)
                     /locus_tag="NOA01_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCI0632941.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(2234..2830)
                     /locus_tag="NOA01_001754"
     CDS             complement(2234..2830)
                     /locus_tag="NOA01_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCI0632942.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(3030..5672)
                     /gene="alaS"
                     /locus_tag="NOA01_001755"
     CDS             complement(3030..5672)
                     /gene="alaS"
                     /locus_tag="NOA01_001755"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCI0632943.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            6060..6491
                     /locus_tag="NOA01_001756"
     CDS             6060..6491
                     /locus_tag="NOA01_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCI0632944.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(6536..7885)
                     /locus_tag="NOA01_001757"
     CDS             complement(6536..7885)
                     /locus_tag="NOA01_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCI0632945.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(7955..9118)
                     /locus_tag="NOA01_001758"
     CDS             complement(7955..9118)
                     /locus_tag="NOA01_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HCI0632946.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            9247..9570
                     /locus_tag="NOA01_001759"
     CDS             9247..9570
                     /locus_tag="NOA01_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632947.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(9753..11102)
                     /locus_tag="NOA01_001760"
     CDS             complement(9753..11102)
                     /locus_tag="NOA01_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCI0632948.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVIAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(11171..12124)
                     /locus_tag="NOA01_001761"
     CDS             complement(11171..12124)
                     /locus_tag="NOA01_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCI0632949.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(12226..13416)
                     /locus_tag="NOA01_001762"
     CDS             complement(12226..13416)
                     /locus_tag="NOA01_001762"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCI0632950.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         3986 a         2864 c         2358 g         4459 t
ORIGIN      
        1 aattagcttt ttcttttttt cgtataagga taaggaattg attctgattt gggtccgttt
       61 ttttcaaaat gatcaaaaag gactacttga tctttttttc gcatcatgaa aacagatggg
      121 gctttctcat cttgcaacat catttgcacc ccagtattag ttggcgtcaa agatcgaatc
      181 tgtccgacag atatttctcg gtctttccaa aaagcagcat aacgtataat cacagagttt
      241 gattttaaaa taaaagaacg ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa
      301 acaaaattgc ttttccaata aggacctgtg ttttctaaag aaagaatcag actaaaaaac
      361 agaatgatca aggtcaatga ccaatagatg atcgtatgtg ccagttcagg ctgccatcga
      421 atattttttt tcattctcta acctctcacc gattcagtaa tgatataatc ttaccataac
      481 attagtttga acaaaaagga gaatctcttt ttatgttaaa agtatacatt gatgcctcta
      541 caaaaggcaa ccctggtcct agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag
      601 aacagctatc tatcccgctt tctataggga caaaccatca agcagagttc gaagtctttt
      661 taaaaacttt agaatttcta aagaaaaacg acttacagaa tgaaacgatt ttctgttttt
      721 cagacagcaa aacactagtt tctacggtcg acaaaaacgc aacgaagaac gagctcttcc
      781 ttccctacct tcaaagaatc caagaacttc tatccgaatt ttctttattg atcctgcaat
      841 ggttgcccga aaacaaaaat aaaggagcag ataatcttgc acgtcaggct ctccataaac
      901 aattaaaaga aaaaaagact ctttaaatta aaaactttaa ggagtctttt tatagtttct
      961 catttaatat aaaagataat tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt
     1021 ttcttctatt tgactacgta agattctttc tagttctgta tcaacaaact ggaatttctc
     1081 agggaccact acatcaaatt gcagcgtttt tccttgatga attactctaa aatcatgaat
     1141 attaagccct gtacctgttt caagaagaat ttgctttaac tgccgcaaga tattatagta
     1201 tttttcgtta tcaacaggca ttggatccaa gtgacagaca agttccacat ccaaagactt
     1261 tttaaagtct ttttctatca gatcaataat cttatgagcc acattcaaat tcattgattc
     1321 atctacttcg atgtggactg atgcaaagcg tttattgggt ccatagttat gcaccaataa
     1381 atcatgatag ccaagtatat catcgcaagc atttaaatgc tcttccattt cttcaatctc
     1441 tgctggcgtt gggcgacttc ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc
     1501 actataaagg atatataatg ctaaagcaaa acctatatat ccatcaatac gccatccagt
     1561 tacccattcg attgctgctg aaagcaatac agccagtgta gtaaagacat cattataact
     1621 atctgttgct gtagctctca atgtttcaga atcaatggat tgcgcaataa cctgatacat
     1681 tttgccttgt gcaaatttca aaaagatgga aatgattagg acaaaaaaga cgatgggtgt
     1741 taaagagaca ttttctggat ggatgatttt ttcagcagaa gactctaaaa attgaaatcc
     1801 tacataagta atgatgatcg aaacaaataa cccactgata tattcgaagc gttcgtgtcc
     1861 ataaggatgt tcctgatctg ctggctttgc agcaatttta aatccgatca aagtcaatat
     1921 ggatgaagca gtatctgata gactgttcac tgcatcagcc ataatggaga cacttcctga
     1981 aaaaagcccg atcattagct tagagacaaa caagatcaga ttcgtaacca atcctaagat
     2041 acccgctagt actccttttt ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg
     2101 tttcttagcc aagtttttta ccccttctag tttgcttgtt gctcaggtaa aaagatggag
     2161 aggacgattg ttttcctctc ctctcttcct ttttctatct atcgtgcgtc ctattactat
     2221 taaaagatgg tcattattct ggtttcaacg gtgttgctgc aaataacgct tttgctagta
     2281 atggtgttat gattcccgca cctattgctt ctggtatccc attgattgct gcaacccctg
     2341 ctaaggtttt aaataaaaga gcagagtcta caccataggc atctgccgtt gcacctgtat
     2401 aaaataatcc catgaatcct aacactaaga ctgtattgac caatgaaccc agtacccctg
     2461 caaaggctga gctgatcgtt attgatttat ttttttgata aatccagtgg aaaaccaagc
     2521 ctgctactaa gccaacaagt actctgggaa ctaccgagat aatcggattc gtgaacacta
     2581 atgtatcaaa tggcgttgtt ggcatggcat aggcacgaac aattgtagcg attccccaaa
     2641 acaagccaat caccatgcca tcttttgggc ctaatactac tgcagcaata attactgtga
     2701 tatggataat tgttaaacta atgaatccta atggaataaa ccccagccaa ggaaccatcg
     2761 cttgtacgat gatgattgct aacaagatcg cacgtaagac tagtcgaaag gtcttatttc
     2821 tactattcat tttttcaccc ctttttattt atactaatcg taaaaatttc tgtcactttc
     2881 gtcaactaaa gaccatccga atccgaccat ttgattagat gcgccatcca attgaaaaaa
     2941 gtatgagggc aagacttgtt tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa
     3001 cttttgtttt tctgtcaaac gtaaacgccc tattgttgcg caacccattc tttcgcagct
     3061 ttaagtgcat ctggaatacc agcaggattt ttcccgccag cttgtgccat gtctggacgt
     3121 ccaccaccgc caccgcctac tttcggtgcg attgctttaa tcaagtcgcc agcttttagt
     3181 ccagcttcat ttgattcttt cgtcattgcc gctagcagac tgacttttcc ttcttgttcg
     3241 tttgctaaaa caagaatatc tgaaatattt ttttgtttcc attggtcagc taattgacgc
     3301 agctgattca tatctttcac ttttacttgt gctgcaatga ttgtttttcc attgatcgtt
     3361 tctggatctt tgaatacatc accagcttgt tgattcgcta acttgctagc taattgttca
     3421 ttttcctttt gtagctctct tacttgctgt tgcaattgtt ccgcttttga aacaacttct
     3481 ttcaattgag gtgatttaac aattgttgct acttgtttta gttgtttttc ttcttcttca
     3541 agtagttcgt acgcttcttt acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt
     3601 cctgattcag aaacgatttt gaagattccg atgttttctg tatttgctac atgtgtgcct
     3661 ccgcacagtt cgattgacca gccgccaata tttactactc gaacgtcatg tccgtatttt
     3721 tcaccaaaca aagccattgc acccatgttt ttggctgtat cgatatctgt ttcaattgtt
     3781 tcaactggga tagcttccca gattttttcg ttgacgattc tttccatttc agctaattca
     3841 tctgaagtga tttgaccaaa gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa
     3901 gaaccagctt gatttgcatg ctcgccaagt acttctttca atgccttgtg cagtagatgg
     3961 gttgctgtat ggtttttgat gatccgattg cgcatcgatt gatcgatcaa tagatgatag
     4021 gtttgtcctt cactcattgg tgcttttact tctacggtgt gcataaattg accattaggc
     4081 gctttttgaa catctgtaac aactgctacg atttcgccta cttcattcat gatccatcct
     4141 tgatccgcta cttgcccacc catttctgca tagaaaggtg tttgatcaaa gatcatttga
     4201 gcttcacctt ctgcgacttc attcaaaaga gactcacctt taacgatgac caataattga
     4261 ctatctgatt cttgttctgt gtatccaatg aatttacttt ctactttgat atctgtcaat
     4321 aaagcagatt gtacccccat tgatttttca gtactgcggg cagagcgagc acgttcgcgt
     4381 tgtgcatcca tttctttttc aaaaccagca tgatcaactt tcagtccttc atcttctgcc
     4441 acctcttccg ttaattctac cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt
     4501 cctgctagag tcgtcccgtt ttcgtttttt acttcttgga tcacttgatt taagatttcc
     4561 aaaccttcat tgattgtttc attgaaacgt tcttcttctg tacggataac tttttcaata
     4621 aattcttttt gttgaagtac ttcaggataa taacttacca tgatttcccc aacaacaggg
     4681 actaaacgat ataagaatgc ttgttcaata cctaattttt tcccatgcat gacagcacga
     4741 cgaagcaaac ggcgtaagac ataccctctc ccttcattcg atggcaatgc accgtcgcca
     4801 atagcaaatg aaagggcgcg aatatgatct gcgattactt tgtaagaaat atctgttact
     4861 gggttttcac catattttac ttgtttgctt agcttttcta cggcgtggat aataggcata
     4921 aacaagtctg tctcaaagtt cgttgacgca tcttgaacga ttgagaccat gcgttccaat
     4981 cccatccccg tatcaatgtt tttatgagga agtggttcaa atgtgttatc aggcttatgg
     5041 ttgaattctg aaaatactaa gttccagatt tctaaataac gttcattttc tcctcctgga
     5101 tagttttcag gatcgtcttc tgctaaatcg ttataagctt ctccacggtc atagaaaatt
     5161 tccgtatccg gaccacatgg gcctgcaccg atatcccaga agttgtcttc tacatcaata
     5221 atgtgatcta gtggcaggcc aacttcttca tgccagatgc gttttgcttc tgtatctttt
     5281 ggataaactg ttacataaag tttttctgga tcgaatgcca tccattttgg atcagtcaaa
     5341 aattcccatg cccagtggat ggcttcattt ttgaaatagt caccaatcga aaaattaccc
     5401 agcatctcaa acatcgtatg gtgccgtgct gttttaccga cattttcgat atcatttgtt
     5461 cgaatagatt tttgggcatt tgtgattctt ggattttctg gcacaactga accatcaaaa
     5521 tattttttca atgttgcaac accagagttg atccacaata aggttggatc gttcacgggg
     5581 atcaaagacg cactaggttc aatcgtatgt cctttagatt ggaagaaatc aagaaacatt
     5641 tgtcgaactt gtgcactact taattgtttc atgtcattac ctgctttcta taaattaaat
     5701 acaaaaaaac accatcacag ccaaggacga aaactcgcgg taccaccttg attgcaatga
     5761 tgtgtaatca ttacctctta aactcgataa cgctgagctg cgtcggtatt tctaccaaaa
     5821 tagaaaaagg gagcaatttg attagtgact attctttttt cagccaagaa agaattttct
     5881 gtgagtctta aaaaacaaat catatccttt tcgtacttaa ttagtatacg cattctattg
     5941 atcactgtca atatctcaat ttttattttc atttcctttt ggtcatactc cacgattttt
     6001 ggtacgataa gaataaagga atcaatacat ctctgctatt catgacaagg aggaatatga
     6061 tgaaaaaaat agttggagcg gatgaatgga tacgcgcagc tagctgcttt ttgagatatc
     6121 aagtttttgt attagaacaa gggattcctg cagaactaga attcgacgga aaagatatcc
     6181 cgcaacaaac ctattatcta tttcttgatc aaaaaagacc agtagctact ttgcgttatg
     6241 aaaagaaaac acctacttgc ttcaagccgg aacgattttg cgtagcaaaa gaataccgag
     6301 gaaaagggtt aggcggtcag ttgcttgaag ctgctgaaaa acgggcggtt tcagaaggtt
     6361 gtcagactag ttatgtagta gctgaaatga cggctgtaga tttttatgaa aaacaagggt
     6421 atcatacttg cacagaacct tttattgaag atgaagtact ttgtatcgga atgaaaaaaa
     6481 gtctagctta aaaacaagag attgtgacac gccttttgtc acaacctctt gttttttatt
     6541 tatgtgaatt tttcttattt ttacgagcag tacggctttg ctgtctgcgt tcgattttgc
     6601 gttctttttt atttttttct gcaattgccc aatcgatttt tttcttataa ccaggcttga
     6661 tctttttctt tttcttcttc acaagaccaa tcagcgtggg atctaataca tcttttgatt
     6721 tttctcgttt ggcacgtcta ttacgatcgt atgttttgac gatttccccg tttttgattt
     6781 cttttggttc aaatgagacg cccaattttt ctatcgcatc aatggcttgc tcatcatttg
     6841 gcgaatataa cgtaatggcc gttcctttta agccattacg tccagtacgt ccgacacgat
     6901 ggataaagaa gtctagatct tctggaattt ctgcattaat aacatgagag acaccttcga
     6961 tatcgatccc tctagcagct aaatcagtcg ctacaacata ttgatactcc agattctgta
     7021 cttgtcgcat cactcgtttt ctctctcttg gggtgatgtc tccatgaatc tttgctactt
     7081 tcaagccttg gtttttcaag taatcggcaa tttcatccac atgctgtttt gtattggcaa
     7141 atacgattgc taaataagga tgaccaattg tcagcagttg ataaatcatt tgattctcat
     7201 ctttaccttt tgttgaaatc agccaatttt caatgtcttc agaaataact gcttttggtt
     7261 tgatttcttc aatgattgga ttttccatgt attttcgtaa gaatggacga agtttttctg
     7321 gaatagtggc agaaaaaact aaaaactgca gcttttctgg taatcgactt gcaatttggt
     7381 ctacttcatt taaaaaaccc atatccaatg tcatatccgc ttcatcgaca acaaatgcaa
     7441 atgcggtgtg aatcttcagc gcttgttcat tcatcatgtc taatattcta ccaggcgtgc
     7501 ctactacaac ctgtggctgt tggtgatgca gacgatttaa ttgacgctgt ttgtctgtac
     7561 cgccgacaaa attcgagaca cggatctccg gtgtagaaaa tttagcgatt tgtgtagctg
     7621 cttcatagat ctgtgcagct agttccctac ttggagctgt aataacaact tgtacttcct
     7681 ctttttctgc attgattttt tcaaacaaag gcaacaaaaa cgtatgtgtt tttcctgtac
     7741 cagtttgaga ttgcccaatg atattttttc cttttgcaat taaaggaatc aaacgttcct
     7801 ggacctctgt gggctgtttg aagccctttg cggttaacgc ttcattgata aacggtttga
     7861 agcggaatat atcaaaatta ttcattcgtg cacctcttct ctttaaatta tcacgacttt
     7921 ggcattataa cacagacaaa gcgtttttgc tcagctaatt tcgagaatca tcatcctttg
     7981 tttggccaat cgtgctaaca gtatatgtgt gactagcttt ttcataaaac agaatccgtt
     8041 tttttctcaa aaatgatagc aagatattta aaataaaaat accttgtagt aacaagacac
     8101 cgagagaaat gaatactgca ctcatctgca acaccgtatt cgttgtcgaa ttcatgattt
     8161 gctgaagcaa ctgccccaac cagcttaaac cataaaaaag aaagtacaga tacccgtagc
     8221 ggatcatcaa tgcttttaaa gaaattcgtt cgcggtcttc ttccaccaaa cggatacgca
     8281 caattttttt gccaagtgtc catccattgg ttataaacgg tacaatcata aagtataccc
     8341 ctacttctaa taaaagccac cagatatgag aagtaactgc ccctgaatcg aacgcaattt
     8401 taaagaaaat cgccgctacc aaattaataa gagataaaaa tgtccagtcg atcaaataag
     8461 cgaccaagcg tcgagtcaat gagacagtct ttcctttttc ataagaaagt tcatccattc
     8521 gtcttcttgt aggaagcata aatgtaaata acggcgtaac gacaaaacca atgattccac
     8581 ccaatgtatt tgtcactaaa tcattcacat caaataagcg gtatggtctt gggtagataa
     8641 aataaagtcc acttagctgc gtcaattcaa aaaatagaga aaggcagaaa ctagcaatca
     8701 tcgttttcat aaaagaaagc cggaaataat atcttaaata aatgccaaat ggtatgagta
     8761 ataaaacatt aaaagctggt tccaaaaata cactctgctt cattgccggc aggtaggtac
     8821 taggttcaga caagaccaat accgtgttgt ctctgaaatg ttgccatgaa gcaaataaat
     8881 gcagctccat ctttggccct gtcatttggg caaccgtctc ctttgccgga agtggtaaga
     8941 tcaccaaaaa atacgcacac aataaataaa aaacgaaaga atacaaaata actgcccttg
     9001 ttactagaaa tgttccatac tttcgatatt cataaataaa aaaaacagat gagatcgcta
     9061 aggctaaaaa tggaaaaaca atcagcgcaa aacgaatcgg ttcgatataa cttgccatat
     9121 gactcctcct tgattctttg tatctctctt atcttagcag tcctttgctg tttcaagcaa
     9181 gtaacgaggc tattatttct catttctttc aaagtaaagt aagataaaca caaagggggt
     9241 gtcgagatgc tgaatatgta ctttgttttt ggcgtaccta tttttttact gttcctttat
     9301 gctacgatcg cttatgtaag aaaaagaact acaattcatt atttaggatt tattctcctg
     9361 attatttcgg gtttcatgct agtcttcaat cttcagactt ggcagcaagc acttcttgaa
     9421 atggataaaa tgactccaca tgctttgtct aaagtgcttg gctaccctgt ctatctgatt
     9481 tggcttccca ttttcatttc tggttgcttg gttttattaa atatttatcg cggcgtacgc
     9541 cgaatagttc agttacgcaa atcaaagtaa aagacagaaa tcggttgagc ccgatttctg
     9601 tcttttactt tgctatttca tcgtagacaa aacgacattt acgtgatcaa aattaacgag
     9661 tacttttgac atttcttcca cgtactcctc atttaaataa aatattttgt ttggcagaga
     9721 agtgcagttc taataaaatt cgaatgtttt agctatctac gttttaaata gagataaatc
     9781 gggataccag caagtgtcaa tactatcccc atcgaagcta aaaaggtctg agtgataagc
     9841 gtgcttgata cgataaaagt ccctccaatg attgctacta gtggaataaa cggatacatt
     9901 ggaactttgt atggtcgaaa caaatcaggt tcttttttac gaagaagaat gacaccaaca
     9961 aagaccatcg tataaaaaat ccaaatgaca aagatcagca tatccgttag tgtatcaaaa
    10021 ccaccgatca tcatcatgcc aatagcgatg actagctcta agatacctgc ggctacagga
    10081 actttagttt tatcatgaag tttagcaaag agcttgctaa aaggcaggtt gttttctttt
    10141 gcaataacat aagggagacg cattccagta agggtatagc cattgatggt tccataaaca
    10201 gaaatcaaga taccaactgt gaccagtctt ccaccgaagc cgccaaaaat catttttgca
    10261 acgtcacttg ccgcattgct gtttcctgcg actccgtcga tagatgcggt tcttaagaac
    10321 accgcattta ctaataaata aacgatcatg attccgatga ttcctaaaga aattgctttt
    10381 ggcaaatctt tcgccggctt tttcaattct cccgaaatat ttcctacgtg tatccaaccg
    10441 tcgtatgcga acatcgttgc tagcaagccc gctcccaagg ctgagaaaaa tgaaagattt
    10501 tctcctgctt ggatcggaaa cagttggaag tctactcctt cttgtcggaa caaaccgaaa
    10561 atgacgatca caaacaaagg aataagcttg cagacaagcg taatcgactg aaaagcccct
    10621 ccagctttag aacctaaaaa attgatcaac aggatcgata cagcagctgt aacagctacg
    10681 ggaacaatca ttgactggga aagaccaaat agattgacga actgtgtacc gaaaatgatc
    10741 gacaaggctg ctacatttgc tggaaaataa atcacgactt gcgcccagcc caataaaaaa
    10801 gctgctgtat ttccgtaaat ccgttcgata tatttaatca tccctcctgt ttctggaata
    10861 gcagctgcca gttcagctcc tgttagtcct gcacagacag aaatcatacc accgagaaac
    10921 caagaaaaca tatgcaaact ggcggatcct gtaacttctg ctacgcttgc ggctttaaaa
    10981 aatacccctg caccgatcac tgtgcccata acagtcgata aagcagtaaa aaatcccatc
    11041 gtacgtttta attcattttc tatttttact tcttttactg gcgtggcttg atattcttcc
    11101 atcccttgtc ccctttcata ccatagccaa tcccaaaagt ataaaaaact ttttggatgt
    11161 ggttcttgtt ttataccgtc tctaccaatt tttctttcag tttttccaac atatcctttg
    11221 tcatcacatc taagtcgtat ttcggcgaaa agccccattc ctcttgcgca caagaaacat
    11281 ctagtgaatt tggccaagat tctgctattg actgacgtac aggatccaca tcataatcca
    11341 tttcaaaatc tggtatgatt ttttgaatag atgctttgat ctcttccggg tcaaaagaca
    11401 tcgccgatat attgaaggca tttcgatgaa ccaatcgatc aggatctgca ttcatgagtt
    11461 ttataatggc atcgatcgca tctggcatat acatcatatc cattgatgta ccttgatcaa
    11521 tgaaactagt gtactttcct tctttgattg ctgaataata aatatcgact gcgtagtctg
    11581 tcgtaccgcc tcctggcaag gttgcataag aaatcaatcc agggaaacgg acaccacgag
    11641 tatctacgcc atatttcaca taataataat cacatagtag ttctccagct accttcgtta
    11701 ccccatacat ggttgtagga cgctgaagag tatcttgtgg ggtatgatct tttggagtat
    11761 ttggaccaaa cgcgccgatt gaacttggcg taaaaaattt cagctggaat tctctagcga
    11821 cttccaatgc attgaccagc ccagtcacgt tcaaattcca ggctaattgt ggttttgctt
    11881 ctgcaacagc tgacaaaaga gcagccaaat gaatcaatgt gtccacttga tattgttctg
    11941 ctatgctgcg cattttttcg taatccatta catctaggat ctcaaagata tcgtttttct
    12001 caatgacttc aggcatgcgt atatctgtcg caatgacatt ttcactgcct agttcttgac
    12061 gtagccgtgt gatcaactct gaaccaattt gtcctaaaca tcctgtcacc aatacttttt
    12121 tcatgatgat cctccctgat cttttttact actatcttca gatgattaac aatatatgcg
    12181 tgaacatgtt ctctaatttt aaatagacga aaaaactaaa tttagttaaa taatttccat
    12241 ttcttttcct attttttgat aaactgctac tgcttcatca agcatttccc tcgtatgttc
    12301 cgcagtcggc atatttctga tcctgcctgt cccaagaggc acagttggat aaacgattgg
    12361 tttagcataa atcccatgtt cgatcagctt cttagagaaa gtctgagtca ctttttcgtc
    12421 tcccaaaatg actggagtaa taggtgtttc agacataccg atgttatagc ctactttttt
    12481 caattcttct ttaaaataat tagcattttc ccaaactttt tcgacaagtt ccggatgttc
    12541 ttgcatcaaa gtaatcgaag ccaatgcagc tgcagctgca cctggagtta gcgaagtaga
    12601 aaataggaat ggccgacttc tagctttcaa ccagtcgatt agtgttttcg atccagctac
    12661 ataaccccca acaacaccta tagctttcga taacgtgccc atttgcatat cgattttgtc
    12721 gcttaatcca aaatgcttga cggttcctgc accttttcca gttacgcctg agccgtgcgc
    12781 atcatcgacg taggtgatca gtccatattt ttcaacaatc ggaataattt ccggcaatcg
    12841 tgcaatgtct ccatccatgg agaaaacacc atctgtgata tacatgattt ttttatattt
    12901 cttactttct atcgcttcct ttgctttttt ctcaaggtct ttcatatctt gatgtttaat
    12961 tcggatgatt tttgctccgg ataagcggca accgtcaata atagatgcat ggtttaattc
    13021 atccgataag atagcatctt cttttgtcat tactgcagag atagcgccca tattgcagtt
    13081 gaatccagat tgaaaggcga ttgctgcttc tgtccctttg aattctgcga ttttttcttc
    13141 taattgctga tggattttca gcgaaccatt gatcgttcgg actgctcctg ctcctacacc
    13201 ataagtttcg gttgcttcaa tacatgcttt tttcaattct tcgcgattgg caaatccaag
    13261 ataattgttg gatgctagat tgatcatctt ttttccgtct acgatgatac aagcaccatt
    13321 ttcactttct agtatgtcga tcgtattgta taaccccttt tctttcagtt gatccaattc
    13381 tttctctaaa tacattgtta gttctttgga agacattgtc tccacctcca tattcatctg
    13441 tcttgcagaa cataccaaac atatatcgtg tcattcatct acaaagcaag tatgctatat
    13501 ttttgataac gcttacactg attatgagaa ttatttaacc acatttttta tttacagtat
    13561 ctttttactt tcaaatgact tatatagtat gaacaattac cttttagata atgatctatt
    13621 ttactctacc tgggcagata aatacaacac aaccattgat agaagcc
//
LOCUS       DAIGOK010000075        13515 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.075, whole genome shotgun sequence.
ACCESSION   DAIGOK010000075 DAIGOK010000000
VERSION     DAIGOK010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.075"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            215..568
                     /locus_tag="NOA01_001763"
     CDS             215..568
                     /locus_tag="NOA01_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632951.1"
                     /translation="MAKIDDYRSDMIEYIELFAKNSNNNIIIQTGGATICGTPIDFDA
                     EVKVNPLIDAMMDSFAEFRSKKIDDIEKDDEQSLVVKSIFLKDVTIIGERTTNIPFLV
                     VFADQISAISLGNLE"
     gene            complement(968..1435)
                     /locus_tag="NOA01_001764"
     CDS             complement(968..1435)
                     /locus_tag="NOA01_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HCI0632952.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQPR
                     EPKVTASYSLVPPSTADHIFEAERMLIDKEEAQEEFEYLHKLFVRGYSAIQHPHKPDV
                     TERRKKIFYDRYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            complement(1510..1950)
                     /locus_tag="NOA01_001765"
     CDS             complement(1510..1950)
                     /locus_tag="NOA01_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCI0632953.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            complement(1980..2273)
                     /locus_tag="NOA01_001766"
     CDS             complement(1980..2273)
                     /locus_tag="NOA01_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632954.1"
                     /translation="MKLKDGFYTDAYGIGGLMMDLPTKNPIKQEESEIKVGDMVRCEA
                     EGFIYPFRGYVEHLYNHSAIIRIENTMECDKRLAKSKENLAVVRLVDMEVIKS"
     gene            complement(2270..2473)
                     /locus_tag="NOA01_001767"
     CDS             complement(2270..2473)
                     /locus_tag="NOA01_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HCI0632955.1"
                     /translation="MNTRHRRVAKLRKQELNVLKTKFEKEYGISAEETYKVASQFVAD
                     ASETIRKFGISILNDDRKWEAER"
     gene            complement(2470..2787)
                     /locus_tag="NOA01_001768"
     CDS             complement(2470..2787)
                     /locus_tag="NOA01_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HCI0632956.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLYQDRFKFVEKYREILDEYME
                     EAE"
     gene            complement(2787..3086)
                     /locus_tag="NOA01_001769"
     CDS             complement(2787..3086)
                     /locus_tag="NOA01_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632957.1"
                     /translation="MDKKAAMKRIIELTHSENWQEDKEIVAEVQKIGKSMWTEKPKRK
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            complement(3089..3250)
                     /locus_tag="NOA01_001770"
     CDS             complement(3089..3250)
                     /locus_tag="NOA01_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCI0632958.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIVNL
                     RREIRRLEE"
     gene            complement(3265..3624)
                     /locus_tag="NOA01_001771"
     CDS             complement(3265..3624)
                     /locus_tag="NOA01_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HCI0632959.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVIDVKGMQTKDFKIKAKLFCHKYQV
                     PLILAKKYRNTFKEERF"
     gene            complement(3621..4472)
                     /locus_tag="NOA01_001772"
     CDS             complement(3621..4472)
                     /locus_tag="NOA01_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCI0632960.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSFVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            complement(4489..5319)
                     /locus_tag="NOA01_001773"
     CDS             complement(4489..5319)
                     /locus_tag="NOA01_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HCI0632961.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLGSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSNEHLRLAKKLQSNLTEDFPKEMTKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            complement(5322..6008)
                     /locus_tag="NOA01_001774"
     CDS             complement(5322..6008)
                     /locus_tag="NOA01_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632962.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVTNFKNKYQIKGIKLSH
                     ETIKKLRIGG"
     gene            complement(6014..6685)
                     /locus_tag="NOA01_001775"
     CDS             complement(6014..6685)
                     /locus_tag="NOA01_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HCI0632963.1"
                     /translation="MSENKGTTTFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            complement(6678..7018)
                     /locus_tag="NOA01_001776"
                     /pseudo
     CDS             complement(6678..7018)
                     /locus_tag="NOA01_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7196..7894)
                     /locus_tag="NOA01_001777"
     CDS             complement(7196..7894)
                     /locus_tag="NOA01_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HCI0632964.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFSEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNIKVMEVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDTVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDENE"
     gene            7949..8614
                     /locus_tag="NOA01_001778"
     CDS             7949..8614
                     /locus_tag="NOA01_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632965.1"
                     /translation="MSLIDFIKQFDWNNIVKTIKDFILPITSLVFSTFAFYISYTDRK
                     NKKFNLKLDFFAECEEWLIDRESDSKPDVYHQNKFRIIDSVLLTNNSSLPVTIIEFSI
                     SGIPDHLNAFTMIGDNYSVTVKSMYDELPHGIRAYSGKSLKKGADLSKFPPLSLPITI
                     PPYESKITTLVFRYDESLVEKNITINVLTSRGTAKFNRFVSSSQISQLDTGYAPPQLD
                     EFD"
     gene            complement(8712..8882)
                     /locus_tag="NOA01_001779"
     CDS             complement(8712..8882)
                     /locus_tag="NOA01_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632966.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEYKTKKPVGRD"
     gene            complement(8869..9207)
                     /locus_tag="NOA01_001780"
     CDS             complement(8869..9207)
                     /locus_tag="NOA01_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HCI0632967.1"
                     /translation="MQYLEAKIPVPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            complement(9223..9426)
                     /locus_tag="NOA01_001781"
     CDS             complement(9223..9426)
                     /locus_tag="NOA01_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCI0632968.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            complement(9441..10187)
                     /locus_tag="NOA01_001782"
     CDS             complement(9441..10187)
                     /locus_tag="NOA01_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HCI0632969.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            10265..10573
                     /locus_tag="NOA01_001783"
     CDS             10265..10573
                     /locus_tag="NOA01_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632970.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            complement(10544..10801)
                     /locus_tag="NOA01_001784"
     CDS             complement(10544..10801)
                     /locus_tag="NOA01_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HCI0632971.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(11099..11239)
                     /locus_tag="NOA01_001785"
     CDS             complement(11099..11239)
                     /locus_tag="NOA01_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632972.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            11539..11859
                     /locus_tag="NOA01_001786"
     CDS             11539..11859
                     /locus_tag="NOA01_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCI0632973.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENK"
     gene            11877..12308
                     /locus_tag="NOA01_001787"
     CDS             11877..12308
                     /locus_tag="NOA01_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCI0632974.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            12435..13019
                     /locus_tag="NOA01_001788"
     CDS             12435..13019
                     /locus_tag="NOA01_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HCI0632975.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            13133..>13515
                     /locus_tag="NOA01_001789"
     CDS             13133..>13515
                     /locus_tag="NOA01_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Arm DNA-binding domain-containing protein"
                     /protein_id="HCI0632976.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVI"
BASE COUNT         3906 a         2586 c         2137 g         4886 t
ORIGIN      
        1 ccactataaa tttcttttct tgcaattatt tttaatatat gctagattat caaccgatat
       61 agtcactgcc tgtactagcg gaaactagtg caggtttttt gttctattta ctcaaaagtt
      121 attacgataa attaatattg tgaaaataaa tactaagtgt ataattttat ttatcagcga
      181 gtggtccgct gaaataattt aaggtggtaa aaaaatggca aaaattgatg attaccgttc
      241 agacatgatt gaatatattg aactcttcgc taaaaattct aacaataata ttattattca
      301 aactggagga gcaacaattt gcggaactcc tatagacttt gatgctgaag taaaagtaaa
      361 tcctcttatt gatgctatga tggattcttt tgcagaattt cgctcaaaaa aaattgatga
      421 tatagaaaaa gatgatgagc aaagtttggt agtcaaatct atctttctga aagatgttac
      481 catcataggc gagagaacta ctaatatccc atttttagtt gtttttgctg atcagatttc
      541 tgcaatttct ctcgggaact tggagtaaca atagattcat ttaaattatc tatattgatt
      601 aaattgctaa tctctcggct attacaaata gctgagagga tttcttttat ttctcctgaa
      661 gtaccttcaa tcgataattt catattctta ccctccaaca cataatttaa tagacagcag
      721 catacgaaga attcagaagg agttgagttc acatccttat tcttaatatt tccgctgctg
      781 tctatcgagg cttaatttaa aacgatgagg gagatttcct cccttacatt ttattttgtc
      841 taagacctat cactaatctt tcgacactat cataatacaa cgttgaatag gtaagtgatt
      901 ggtataaaaa aggtataaaa tggaaaccaa attggtaata aaagggtata aaaagtgtaa
      961 aaactggcta cttgaaagca accagttcta acgatgaagc aaattggatg ataattctgt
     1021 ttgattctac tttaaccgat tcttcgctag tattattcct ttgagcagtt acataaatgg
     1081 gcagaccatt gatataacga tcatagaata tcttcttgcg cctttcagtc acatcaggct
     1141 tatgcggatg ctgtatcgct gaatagcctc gaacaaacaa tttatgcaga tactcaaatt
     1201 cttcctgtgc ttcttcttta tcgatcagca ttctttctgc ttcaaatata tgatcagctg
     1261 tagaaggtgg aaccaaggaa taagatgctg tcacttttgg ttctcgaggt tgccctactc
     1321 tacatctagc tgatagatat gctgaaagaa acacagcgac attatgtttt gtgcgttcca
     1381 tatcaacatc tttcgcgctt ggtgtctcat atttctttac atcgaaaagt accatcctct
     1441 gattccccca attatgatat aatacttatg tcggaaatat tattcatagt cggaggaatc
     1501 cgactttttt tattttgtac gtgaaatgag ttctcctgat ttatacgcct cagcaaattc
     1561 aaccaaagct actgccttca ttctctcgat agttctatcg gaatatccta atttttctcc
     1621 tatttgatac attgacatct tattaggaaa acaataacta taataaagta cctggaaatg
     1681 gattctactt agccgtgtta atgcttcgat aaccgaatct cgagtactta tcgcttcaat
     1741 gatttcttcg ttttgactgt caattgtgaa tttcaaattt ttggtaacag tcatcacgga
     1801 aaagtctatt ttcactggtc cgaccaaacg ttctaaattt ctgtatcttt ttagtaatcg
     1861 tctagcattt ttccttgttt gcttaatatc tatattctct aaaatgttca caattgcctc
     1921 ttctccttcc agttgtggaa taacgctcat tgttgtagga tagttgtaaa aaagcaaaat
     1981 taagatttta taacttccat atccaccaat ctcaccactg ctaaattctc tttgcttttc
     2041 gctaaccgct tgtcacattc catcgtgttt tcaatgcgaa tgatcgctga gtgattatag
     2101 agatgctcta catatccacg aaatggatag atgaaccctt ctgcttcgca gcgaaccatg
     2161 tcaccgactt taatttctga ttcctcttgc tttataggat tcttagttgg tagatccatc
     2221 atcaaaccac caattccata agcatcagtg taaaatccgt cttttagttt catctttctg
     2281 cctcccattt acgatcatca tttaatatcg aaatcccaaa cttacgaata gtctcactcg
     2341 catcagcaac aaactgactt gccactttat atgtttcttc tgctgaaatt ccatattctt
     2401 tttcaaactt tgtctttagt acattcagtt cctgttttct tagttttgct actctgcggt
     2461 gtctggtgtt cattccgctt cctccatgta ttcgtctagt atctctctat acttttctac
     2521 aaacttgaaa cgatcttggt atagtttctt gctccaattt gtttgccgat ccagctcacg
     2581 catctgatcg aacccttttt gaatttcgtt gtaatagaat tcaatgtttg ctgctgcttt
     2641 ccaatgcctg ctacttcgca ctcctgctcc tgtttcagcc atttccaact tgactaattc
     2701 cgctcgttct tttgattttt tatctttctg aatcttcatc atgattttct tgaggatgat
     2761 atcactgtat tgtgtaatga gatccattat ttctcctcca catacctaaa ttgtcgtcct
     2821 tttgaatcaa tccataagct cctagctcta tcccagatga tgtttttgct cagaccagta
     2881 atttcagata actgttcagc agtacctgtt actagaattc gatcaccatg ccagattgca
     2941 atttttctcg gcgttttccg tttaggcttt tcagtccaca ttgatttacc gattttttgg
     3001 acttctgcaa ctatttcttt gtcttcttgc caattctcag aatgtgtcag ttcaatgatt
     3061 cgtttcattg ctgctttctt atccacgctc attcctccaa tctacgaatt tcccttctta
     3121 aattcactat gtgtaaatcg attgcctttc tcgccgtttc attgaccatc actgcctttg
     3181 ttcgttccag atcgtcaatc tcccgttgaa ggcttcgaat acgcatttga atcacttctt
     3241 ctgttgtcat gatggaccac ctcgttaaaa ccgttcttcc ttgaacgtat tccgatattt
     3301 tttagctaaa atcaatggca cttgatattt atgacaaaac aattttgcct tgatcttaaa
     3361 gtcttttgtc tgcatccctt taacatctat gactttgaca agcttgccgt ttttataaaa
     3421 tgtaaagtcg ggaatatact cgatcttgcg atacttcttt ccgtctagtt caaatttcgg
     3481 catcagctca aatcgttcct gaagttttac tttccagccg ttcgcttcag cttgccataa
     3541 ggctagatcg tagtactctg cttctgcgat agaatcgaac ttgatacctc gatgaacagt
     3601 ttttttatta cggtatttat tcatgcgata ctacctttca ctggttttat gcgcttgtct
     3661 gctgtttgtt ggaatttcag cgcataacct tctgaattct taaatatcct agaaacaatt
     3721 ctttcgccgt aggcttctct tagttcagga ccagataagt ttgttgtgat gatcgttgcc
     3781 ttgttctgtc tggcttctaa gagcgtgttt aacgtgttgt ttgtaaactg cctactattt
     3841 gataccccac tacctaattc agctccaata tcgtcaaaaa ccaccaaatc agttgtttta
     3901 atatcggcta taagcgatcc ttcaatttct tttctcagtt cagcattgtt ataagagaac
     3961 tttatttgct ctaataactc ttgatagctt ataaaaagta ttttcttgtc ataatttgag
     4021 cgctcaagta tttcccaagc tgtcgccatt gacaagtggc tttttccgct tcctgatttc
     4081 cctgatagaa tgaaatgtgc aggatggttc agtaggacat catttacaaa gcttttagct
     4141 ctttctaaag caattttcgt ttcttggtcc actacgtgat aattctccat tttgcattta
     4201 aacaaagttt tatctgttaa taccgaacca ttttgaaaaa aactcaatgc tcgtgctttt
     4261 aagctgtcgt tatatatccg ttcggtctgt atatcctctt tcacacgtaa cgctttataa
     4321 ccacaactca tgcatgttgg tttacaacgt tctgaaccat ccttattttt agctcgccaa
     4381 ctatacaaag gttcgctaca ttctggacat tttccgcttt gcactaatac tcttcttatt
     4441 agcttctcca tagcatttgc taggctttcc atgtgatgca tctccttttt aaattggcaa
     4501 gtcgtcatat tcactaggat tgctgtactg tagtttttga ctttgctttt tatgattctt
     4561 cttatctgct ttgatttcga atttgagctt ctcaaatttt tctctcaatt tcttagcact
     4621 tctaatattt ccaaaccaaa attcatttgt aggtagccaa ttgatcacat actcaatcgc
     4681 ttctatagat gctttatctc tttcttccat caacctgatt gtgtctgccc atttttcgat
     4741 atctacttta gtcatttctt ttggaaaatc ttcagttaaa ttactttgca actttttagc
     4801 aaggcgtaag tgttcgttag aatacttacc tttcttttct tctttatcta tatctttatc
     4861 ttcttctata tctttatctg taccgtcacg tgacgtcacg ctaacgtcat tttccaattt
     4921 gagacgttcc tgtctcttcc tttctctgta tttacggttt ctttcagcat tttttagcct
     4981 tactttatcc atcccctcga tattttgatg tttttcccaa ttactgatgg caattagtcc
     5041 atcactgcct agatcaatca tgttgaaatt tgccaatgtg gttagtgcta agcgaaccgt
     5101 atttacgttt ttgccaaaca atgtagcaag catttcttcg gtataaggca tgttcctctg
     5161 gatgtatatt agaccatcgt cgttagtctt tcctgctaaa actagtaatc gaatccatat
     5221 aacgatgatg gcatccgact caggaacagc ttgaattaat cgtatttttt catcgtcaaa
     5281 catagtagtt ttaagtttga tccaacttat ctcagccaaa tttatcctcc tatccttaac
     5341 tttttaatcg tttcatgact taacttgatt cctttgattt gatacttatt tttgaaattg
     5401 gtcacaccta ttttgtgttt ctctgtgtga tggattctgc agagtgctgc aaatgtgtac
     5461 tctgaatgat caacttcttt gcgctttcgt cttcctaaag ctttgtcaaa gtgatcaata
     5521 tcagctcctg ttttgccaca gatgcagcag actctttttg taatgcattt gtagaagtaa
     5581 tactcttgat tcgctggtaa aatctcatag ccttctttga aaggaatatg atgttcaaag
     5641 ataaaatcta ggatgatatt tgctaagacg ttagcatcac tcacggttgt attcgattcg
     5701 tctttgaggc ttattttgcg ccctgtgaca ccttcgaaac ggaagtagaa gaattccttc
     5761 cagaagtccg ttggcatgcc tgtatcgatg aaaatatcgc ctatgagcgc atagatgaag
     5821 tttcgttgct gtacagtgaa acgtctagga tcaataaaac gaatttcaat gactcgatcg
     5881 ccatcgtagc cgtcatacat cgtctttagt cgatcaatgt tcacttcttc attgatcgtt
     5941 gcacctatgt cttttccttt gaactttttc agaaccgctg aatatgaatc gattaatggt
     6001 ttaaacactc atatcacttc ttatctaatt cttttctctt agctgctatt gctcgctcca
     6061 tcaaggcaca ttgctcatag cttaactgtt caatagtttc aacgttatca gctaagagcc
     6121 ctaatttatc tgtctgctca ttaacatatt cgattaaggt tttggtcata tctttaccca
     6181 tctgctcatt gaaagcttct agaatcgtct ctagcatgtt taatttcttc gtatcgattc
     6241 taggtggtgt tggaatatct tccccttgaa atacatataa tcccagtccg tgtagagcca
     6301 atgctttcac aaagcatcgc ttcaatgagt tattgatttg cattgcattt ggtttaacaa
     6361 ctggttggtt tcgataatct aaaacaggaa ataattcggt ttccgtgtgt cctttaaccg
     6421 ttactgagac agatacataa gtcccagttt catccataag aaaaggttta tattcctcaa
     6481 caagaaagtc ttgatgagtt ccagaaacaa ctctgtagtg tttatactca ttaatagtta
     6541 ccgttgcctg tggatcattc tttttcataa tctcccatgc gtgagcccaa gataaataat
     6601 caaaatttcc ttttttcttg agtattttat ttaacttgcg actaaaaagt ttttcaaaag
     6661 tcgttgtccc tttgttttca ctcatcaaat tctgcctcca tttcagcaat gtatttctta
     6721 cctgatccgt aataagagat atcaatcaag ttatctctgt catactcttc tagcgcatca
     6781 atcaagccat cttcgatgac atagatatat tcaggttttt ggacttcctc gataaatgga
     6841 taagatagac atgatcccaa atactcacaa aatttcccaa atcgtcttga tcacatgcta
     6901 gttcttcatc cgtcaaaaga tttcgtctga tttttcgatt atttgtttcc ttgatattcg
     6961 atttgcccca actaggatca gtcaaatatt gatctagagt ggaaagttct ttttccatat
     7021 gctaaaatct ccttatgatg tgttttcttt gtgactcttt gcttgccggc ggagtcactt
     7081 ttttatttgt tgccatgctt tttgcttgtc aatatgttgt tggcttagga tgattggttt
     7141 attgtgtctc caccagcgat tagcaattac cgtcccgatt cttagcgctt cagctctatt
     7201 cattttcatc accgaaaagt ctttgttgtc tgttcagttg atcgatttcc atgcggatcg
     7261 cagtttctgg taaccacatt tcaataaatg aaacagtatc atcgaatctc ttacgaggta
     7321 actcgccata tcttgggatt gaaaaggtac gtttaaattc agaccaaaat tttgagaata
     7381 cttttttgct gatttcttca taagctcggc tttctttacc ccctagaact tccataactt
     7441 ttatatttcc tttttgttta atttcaaact cttgttgccc actaattcgc atagtatctt
     7501 taagcatgga aacatctttt ttaacatctt tcatttcttc tagttggtag atcatcatat
     7561 cttcaattgt ttgaggaaca gtattcttgc gaataacatc ttccatttcg ttgaatgctt
     7621 caatgtattt ttgtttgaag taaatagctt tctttcctgt aaaacccata gccaacaaga
     7681 aaaaaccatc tctactaatg aagaaaactc gtcgatttct gccgtatgaa tctggttcat
     7741 taccttcaga aaacatctgt ccaaaattgg acacatcttt tttcagtgca tcaatatctc
     7801 ttaaaacatg ttgatgcttt ttctcaaaac tttctgcaac ttgcaaactc gtagtcacag
     7861 cttctttatt tttcaaaatt actaattctt gcattatttc ttctctcctc tttgatataa
     7921 tgtagataaa aaatggtggt ggtataaatt gagtcttatt gactttatta aacaatttga
     7981 ctggaataac attgttaaaa ccattaaaga ttttattttg cctattacgt ctctagtatt
     8041 ttcaacattt gctttttata tctcctatac agatagaaaa aacaaaaaat ttaatttaaa
     8101 attggatttc ttcgctgaat gcgaagaatg gttgatagat agagaaagtg attccaaacc
     8161 agatgtttac catcaaaata aattcaggat tatagattct gtattgttaa caaataacag
     8221 ttctttacct gttacaatta ttgagttttc gatttctgga attcctgatc atttaaatgc
     8281 ttttacaatg attggtgata attattctgt aacggtaaaa tcaatgtatg acgaacttcc
     8341 acatggtata cgtgcgtatt ctggtaaatc tttaaaaaaa ggtgctgatc tatcaaaatt
     8401 tcctccgttg tcattaccta ttaccattcc accgtatgag tcaaaaatta ctacgttggt
     8461 ttttagatac gatgaatctt tagtcgaaaa aaacattaca ataaatgttc ttacaagtag
     8521 aggtactgct aaattcaata gatttgtttc ttcatctcaa atttcacagc ttgatactgg
     8581 ctatgcccct ccgcaactag atgaatttga ttaggaaatt ctccagtttc ttttacaaat
     8641 tctttgatga gatcattaaa attttctttc aattgttttt cgtttatagt cattaaactt
     8701 tttttcatta gtcagtccct cccgactggc ttttttgttt tgtactcagc ttcatccagc
     8761 cccataaaaa tccaaaccat gtaaacaatc gttcctatca acgcttgtct gcttccccaa
     8821 agtcctaaag cgtagatgat tagcggtgcg ctgaatacta gcgctctatt aaattttccc
     8881 atccgcttgc ctccttaaga tttccgaaaa atttgtttct aaaaattcaa gtgttttact
     8941 tcttaaaaat agatatgtgt ctcttccttc aactggataa tagacaaatc catttttatt
     9001 tttttcgata tcgataatat ttctatatct tgggttcttt aaaactctag aagtaaacca
     9061 atcatatttt ctgttaatcc gttctagcac ttctggcaac gtcatccatc taccagtatc
     9121 atcagctttt tttaactcct cataatccac ttgggagata attacatagc cttctggaac
     9181 tgggattttc gcttctagat attgcattag ctgttcctcc ctttacaatt cgtacatagt
     9241 aataatcgaa tctatgattc tatttgcttc tgcagaagtc tttttaccgt ttaaaattaa
     9301 agataagtag cttttgctaa ttccaaatct ttcagcaagc atggtgtaag ttaagaactt
     9361 cgaactttcg acatattctt tgattttttc tctatctcgt tgagtgattt ctgcaatgtc
     9421 agtcatacta aaactccttt ctaaccaatt tcctctaaat ccatttgagg gtaatagccc
     9481 tctgcgatta ataagttgta aataaacact cgccctttct gtgtccattt ggtattcatt
     9541 acaactttag tgccgccatc ggctttcggt atctcacttg tgtgcgattt tgtgtatcct
     9601 tggttcatat gttttcggta aagtatccat tgtccgctaa ctttatgctg aacacctaac
     9661 tcgtttagca atttgtttag agcaattgcc gacattccgt aatcagctgc aatttgagaa
     9721 gtagctaccg tatctgtcga tgaaagaatc gtatctaagt agctaatctt tggttcgtac
     9781 tcggcaattt gttgttcgag taattggttc ttttcttcta aatccgctgc taaacgcaat
     9841 gcttgtgcaa aactttgagg tacgtttgaa tagctacctg tttttctaat tgttggaagg
     9901 acttcactcg ttacccaacg tttaaatttt ttggcagagg gaagttttga ttttaagatt
     9961 aaactgtaaa ggcctgactc gttaatcaaa acaactgctg ttccattgac ggtgaacgaa
    10021 tcgttctgcg tcttatcctc caagtccaca tgatctcgaa tagctttttg cggattcgaa
    10081 tatcctagaa cgtctgctac atcttttcct acaaaatatg gttcatcatt tactaaaata
    10141 gttcgaactt cgttttgctc gaaattaaaa atttgtggtg tattcatatt tctcattcct
    10201 ttctttggta taatttcctt atcagtgtga caggctgaaa taattgttaa ggaggtgaaa
    10261 atttatgaaa ccagaatttg taattactat gcttgatgga gaaaaaatca aaatatttgc
    10321 cgatactttg gtcgttggat acgatgatgc gtcaaatatc gaagaaaaag atggaatgtt
    10381 ctattcttct caaatttacg tgaatacctt gcaaggtgat attggggtat ctactcttgc
    10441 aacatcaaga ccgcttgtag gtcttatcgg ctttttgtca aaagcaacat tcttttctct
    10501 cggcgatgat atagattcgg atgaaactgt catctataaa acatcagctg ttaaaagtat
    10561 cggtttcaaa tagtaattct tgctcgttga tagcggaagc caacttttct tccaacacat
    10621 cggcgagctt tctttttgtt ggattcattt caaagttgat agtcttgaga agcatttttg
    10681 catccttaac tttttgtcct tgcaattcct tgatgatttt atcggcttgg ctatcaattc
    10741 catgtgttag atcatctatc attttttgta ccttgattgt ttggctcatt tccttgttca
    10801 ttgtttagcc tcctttaata aatgattttt tgtatttttt aatttccata aattcctcaa
    10861 attgctcttt ggtattatta ccacgaccat acatattgtg aaattcttta tggcaactga
    10921 cacaaagtgt aacgccgttt gaaatcgctg cggatttagt tgttgcatgg ctaaatcacg
    10981 aatcacaagt atctactaaa ggcgaatcaa tttcgccaaa agaaattgtt caagcttatt
    11041 tagatattca ttatgctgtt atctatggtc agcttccaga agaccgtaaa aacgatgact
    11101 aaacgtgagc taaaatctct gctatggctg caaccatggc agagtcttct ttatctaccg
    11161 cgtgttccat cgccactttt gcttgtttca gtatgtctaa tttcaaatct tcgatttttg
    11221 ccgagactgt tttttccata ttcttctctc ctttctttta tattcgtaaa caaatttaac
    11281 aactttttaa aaaactgcgt tgactttatt agattaataa tctatactaa atgcatagtt
    11341 aaataagaca tataaacttt gatttaaaaa gctttcttgg cggttggcat ttattaatca
    11401 atagtatttt ttgttgtctt tttagttgtt aaacttgttt acaaaacata gtatagatta
    11461 ataatgtata taagtcaact atttttacat tatttatcta aacttttttt gtaagcattc
    11521 agaaaggttg atttatcaat gaatacttac gaaataataa aggagttagc aaaagaaaaa
    11581 ggattatcca ttagacagct cgaaatgaat ttcgggtatt cgaatggata tttaggaagt
    11641 tggaaaagac aaactcctaa ttctattgag ttagctcggt tagctgatta ctttggagtc
    11701 tctgtagact accttctagg tagagaagaa agagaaaccc ctaaacatgt ggatttatca
    11761 gaaaacgata ctgtattttc ttttgacgga aaagaaatat ctaaggagac aatgcgtaaa
    11821 gcgattgcaa ttgctaaagc tttagaagaa aacaaatagt tggagtgatg gtttatatgt
    11881 atttgaaatt gaaagaaatg ttgagcgaat acaatttgaa agtagtttat atggaaatga
    11941 aagagcctgg tttctactat ccaaaaccca gaatcatttt tttgaatgaa aatctatacg
    12001 gtgaaactgc agaagccttt catttatctc atgaacttgc acatttcagc gcctctcatt
    12061 ttgaattttc agttttgtac gatacttcta caacttttca ctcaaaattt gaaacagaag
    12121 cagataaaat agctattcta attttattaa atatctatat tgagaacgaa ctgactgatg
    12181 aatctcagtt taatctcgaa aaatttatgg agtattattc tattcagaat aagctcagat
    12241 acacttgtta tgctgtctgc caatgctatt ttaagaaaaa atattcttac gcaaggcaat
    12301 atgtatgaat acatcaagaa ttattaaaaa aaccgatttc agcaaaacat taaaaaagcc
    12361 cgtgctggca cacggactca tacctcattt ctgagatcgc aaatatatta taacaagaag
    12421 tgaggaatat ttaaatggca aaaaaagtta tgggtcaaga cggaaaaatg tataaagtta
    12481 gtaaaccttt ttacaaaaaa gtatggtttt gggtattagc agtaatttta attataatta
    12541 ttggttctgc tttaaatggt ggatcagata gtaataaagc aagtgataat ggtggcgaaa
    12601 aagtaactaa atcttcaacc tctgcttcat cttctaaaga agaaaaaagc gataccttct
    12661 ataagattgg tgacactgta aaagttggtg atgctgaata tacactaaat agtgtagaac
    12721 taactgatga aagaaatcaa tttgaagaaa accaacctgc acaagtagta aaaattactt
    12781 ataccgtaaa aaatgatggc gattcagata tccctgtagg tacagatgtg gaagtatatg
    12841 gaccagatga caaaaaatca gagacatatg ccaacgaaaa tacaatggga tctgttgctc
    12901 caggaaaaca aatggatgta actgctcatt tcacgttaaa ccaaaaggga gaaatagaaa
    12961 tccacttctc tcctttagta tcatttgaaa aagcagctat ttttaaagca actgtataat
    13021 aaataaaaaa cacgcccacc gtccaaagca agcgtgttct aagaaaaaca aacctataca
    13081 gtaggcttct ttatagtgcc tattgtatca gagaaagaga gtagattcaa ttatggcaag
    13141 attagtcaaa cgtggaaata gttggcaata cgaaatttca tacaaaaaag atgacggaaa
    13201 atacacgaag ataagaaaat caggatttaa gacaaaaggc gaagcaaaag atgccgccaa
    13261 cgaattagaa tataacttga acaaaggcct taaaggggat cgcaaaaatc tattattatc
    13321 agattacttt gaggattgga tgcaacttta taaagaagga acagtatctc ctatcactta
    13381 tagaaaatac gaagatacgt taatgaacat aaagaaatat atgccagcgg tattgatttc
    13441 tgatttagat agagttggat atcaacgctt tttaaataag tatgcgaaag accatgtaaa
    13501 atccaccgtt attaa
//
LOCUS       DAIGOK010000076        13432 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.076, whole genome shotgun sequence.
ACCESSION   DAIGOK010000076 DAIGOK010000000
VERSION     DAIGOK010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.076"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            156..2054
                     /locus_tag="NOA01_001790"
     CDS             156..2054
                     /locus_tag="NOA01_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HCI0632977.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(2353..3072)
                     /locus_tag="NOA01_001791"
     CDS             complement(2353..3072)
                     /locus_tag="NOA01_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HCI0632978.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            3409..3741
                     /locus_tag="NOA01_001792"
     CDS             3409..3741
                     /locus_tag="NOA01_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632979.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            3728..5731
                     /locus_tag="NOA01_001793"
     CDS             3728..5731
                     /locus_tag="NOA01_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HCI0632980.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            5750..6220
                     /locus_tag="NOA01_001794"
     CDS             5750..6220
                     /locus_tag="NOA01_001794"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HCI0632981.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            6241..6825
                     /locus_tag="NOA01_001795"
     CDS             6241..6825
                     /locus_tag="NOA01_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HCI0632982.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            6840..7826
                     /locus_tag="NOA01_001796"
     CDS             6840..7826
                     /locus_tag="NOA01_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HCI0632983.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            7816..8127
                     /locus_tag="NOA01_001797"
     CDS             7816..8127
                     /locus_tag="NOA01_001797"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HCI0632984.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            8149..9930
                     /locus_tag="NOA01_001798"
     CDS             8149..9930
                     /locus_tag="NOA01_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HCI0632985.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            9923..11299
                     /locus_tag="NOA01_001799"
     CDS             9923..11299
                     /locus_tag="NOA01_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HCI0632986.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            11306..11941
                     /locus_tag="NOA01_001800"
     CDS             11306..11941
                     /locus_tag="NOA01_001800"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HCI0632987.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            11971..13326
                     /locus_tag="NOA01_001801"
     CDS             11971..13326
                     /locus_tag="NOA01_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HCI0632988.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
BASE COUNT         4358 a         2350 c         2988 g         3736 t
ORIGIN      
        1 aaaaatatag aaggaagctg gacatttatt gttcccagct tcttttttgt atagacctat
       61 ttctattttt ttattatatt ggatatatat attcattgaa aatagagcat aacaacttta
      121 taatgttgag tataccaatt tttggaggcg ttttaatgga ccgctattta gatttacttt
      181 cagagaagtt ccctacaacg gaagatgtca tcacggaaat catcaatctc gaagcaattt
      241 tgcagcttcc aaaaggaaca gagttatttt tgagcgatat acatggcgaa tttcctgctt
      301 ttgatcatat tctgcgtatc ggttcaggaa atcttaaaga aaagatcaaa gaccttttct
      361 ctgaacaaat gacggaaacg gagatcagcc aattcactat ttttacggct tatccagaat
      421 atgtgttgac gactgattgg tacaaaaaac aagataaatc ccagctgatc catcgtttga
      481 tcgatctttt acggtttacg acagtcaaat acactcgatc aaaagttcga aaaggattgc
      541 cgaaagaata tagctatatc attgaggaac tcttgtatat cgatgatcgt atcaacggga
      601 aaaaagacta tgtaaaaaaa atcattgaac aacttgtctt gatgaaggaa gaagaaaaat
      661 ttttaacaaa acttgcgcag acgatccaaa aatctgtcat cgatcatttg catatcgtgg
      721 gtgatatttt tgatcgagga actcaagcag ccaaagtaat ggatcgaatc atggattttt
      781 cctctgtaga tatccagtgg gggaatcatg atattttgtg gattggtgcg tattgcggct
      841 ctgaggcatg tttattgacg cttttacgga ttgccgcacg ctacaactat ttatttgaat
      901 tagagaaaga atatggatta aatttacgtc cattattttc ttttgcccat caaacctatc
      961 agaagaatcc agtatttacc cctaaaaaca ggaaagatct atctgaaaga gagcaagaag
     1021 aattagaaaa gatccatcaa gcactttcta tcctccaatt caaatcagaa catcagttgt
     1081 tgaatcgacg acctgaattt aaaatggacg atcgtaaatt gttggaaaaa atcaactatg
     1141 aggaatcaac gatcgtttta gaaggtcagc tctatgagtt gaaagggact tgttttcaaa
     1201 cgatccagcc agatgatcct aaaaaattct tggaagagga acaaaaagtg atcgatagtt
     1261 tgatgtattc attccagcac tcgttaagaa tgcaaaaaca catgacgttt ttgatcgaaa
     1321 aagggggaat gtatctcacg aacaatcagc atgtactgtt ccatggctgt atacctgtag
     1381 atgaaaaggg gcagctctta gcatttgaat tagaccaatc ttatcgcgga aaagaactgc
     1441 tgggattttt tgaaaaacag ataactgaaa gcgcaaaaga tttgactgca aatgaagatt
     1501 tggcgaccga tttgatttgg tatgcgtgga gcgggccatt ttcgccttta ttcggcaaga
     1561 gtaaaatggc aacttttgag cgctattttc tcacagagcc tcatacgcac gtcgaaaaaa
     1621 gcaatcccta ttatcacttg cgagatgaag agtggttcag tgaaaaaatt ttggcagaat
     1681 tcgatgcaaa agaagaaggt tcagcaatca tcaatggaca cacgccagta aaagtaaaaa
     1741 aaggagagtc accgatcaaa gcaaacggaa aagcatttgt tattgacgga ggtctatcca
     1801 aagcttatca aaaaacaacc gggattgctg gctactcatt actatgcaat tcttacggtt
     1861 tccaaatcgt gacacattat ccgttcttgc ctattgaaca acaatttgca aaaaaatctg
     1921 atcaaacgaa tgtaaaaaaa gtgatcgagc agcagctccc acgcaaactt aatcggaata
     1981 cgacaaaagg aatggaatta cagcaacaga ttactcaact ccaaaggctg ttggattatc
     2041 gaaaggacga ttgagaaaaa tgctagccaa cattgctcaa tagactaaaa agcgactgca
     2101 acaaaaggcg tgtcgcagtc tccttcggaa ttagctcaaa aaacggtaaa atattagaag
     2161 ctatttttct gtttatttct ctaatactca ggtcagggcg ctgatttcac gacctcttcc
     2221 ttaaacggtg ttctcctgcc aactcctcgg tcacaagtca caatatccaa caaacatgag
     2281 aagctgtttt ttgaaattgc tccttgtgac tcagagttga acggcaggct cacgacctaa
     2341 tcagaaaaac gattaaaatt cattcacaaa gaaatcgaat aaacgctgtt cagatgaatc
     2401 tacacgatga gaaagttccg gatgccattg gacaccaagg attttgtgca tattatcttt
     2461 actttggact gcttcaatga gtccatcgtt ggaaaaggcg atggctttta acaaaggaga
     2521 aagatctttg actgcctgat gatgatagga attcacaaag tatgaatcag ggagaaggtc
     2581 acttaataaa ctgtccgaaa cgattttgac ttcgtgagtt gcaaactgcg gggcagtggg
     2641 ctgttgttcg tgttttacgg accattttgg atataatgaa agatcttgat aaagtgttcc
     2701 ctccaacacg acattcaata gttgaagtcc tcggcagact gcaaaaatcg gttttctttg
     2761 tttcaatgct tccaagatca atgcctgttc gaattggtct cgttgaatat ttgtttccgt
     2821 cagttttgga tgagggtctt ccatgtaaag ttgcggacta acatcctgac cgcctgcaag
     2881 aatgagtttg tcgattttct caattagttg aggcgctgaa tctggagaca tgactggcag
     2941 aataagaggt aatccttgag catctaaaac tgcatccaca aatccctgcg gtgtatagct
     3001 tacttgattt ccttggaaag tatctgtcgc tcgaatcagt tgattcccgg caatgccgat
     3061 gattggtttc atttttattc cccctaaagt agactaattg taacaaggat ttaaaaaaat
     3121 ttaaactatg taagacttgc ccctatactt atctgttctt gctgtgttac tatctgtaaa
     3181 gagaattttc gtgtgaaatt catgacgata gttgaaaata ttgcttaatt tgttataata
     3241 aaaagtaagc agaaataaaa aggttgggcg taagcttcaa ggatctagtt cttcaagggg
     3301 tgagaaggac attttattga aggggcctgc ctatactaag ttatctttag aggtacatac
     3361 tttcaatgaa aaaagaaagc atgtacctct tttttaaatc gaaaggaagt gaagcaagtg
     3421 ttagaagctt tggaacaaat ccgaaaagca gaagaagaaa atgagaagcg tgctcgaacc
     3481 ctacggcagc agttgaagac ctatgcagaa gaaaaagagc aagaattgga gataatccaa
     3541 acgaacagtc ggcaacagct gaaacagttg gtagaagaaa aagaacaatt cgaaaagcaa
     3601 gaattagaaa aagaaaaaat gaaattgaaa gcagaagcag acaaggtgca agcaactttg
     3661 aaagaccaat atcaaaagca tcagagtcaa gcaatcggtg caatcattga aagggtgaaa
     3721 gaaacttatg gccgtcacta agatggaaaa agtgaccctc atttcagata aaagaaatca
     3781 ggagaggatt ttacaagcag tacaaggaat ccaaaatgtt gaaatccggg atctgtatca
     3841 aaatagaaca aacaaccagt gggtagaaaa atattttcca gataccacga ttattgataa
     3901 agaagcaaaa ttaagcgctt taggcaatcg gctgaaagac atccaagaag ctatccaatt
     3961 catacaacac cacggcgata gccgtcaaaa gaaactccat ttgaaacgtc aagaactttc
     4021 cttacaagac ttggaagcaa attattccga agaaaatttc atgaataaat tagaagaagt
     4081 ccttgattta aaagaaaagt gggaacagct aactgaaaaa cgtgatcgtt tagaagaaga
     4141 ggaagaatgg cttcttcatt ggcaacagct ggatattgct ccaagtatgt atgcaagtaa
     4201 gacgaccatt tttgagatgg ggactgtgag tgcagcaaat tttgaaagtt tcaaggaaga
     4261 actaagtaag cttgatgacg tttatatgga agaaatagat tatacgccaa aacaagttta
     4321 tctttcgtat gttgctttaa aaaaatcagt tgctgctata gaagaaattg ctgggaaata
     4381 cgggttcaat aaagaaacgt atccttatga acaaaatcct aaagaaaaat tgcaagaaac
     4441 caaacagcaa ttatccattg ttcacgagca gcaaaaaaag ataacgacag cgttaggtca
     4501 gtgcagcggc tatatccaag aatttgaatg ggtagaagaa gtcacacttg ccgttgcaga
     4561 acgggaaaaa gtaaaagaac ggtttatcca tgccacttat ctgatcgttt tgcaaggttg
     4621 ggtagatgca gaagaaaaac agaatttgat gcatgtgtta aacgaaactg tatcagaaga
     4681 agatatatac gttagtttcg aaacaccaaa ctcagaagag attcagcagg aagtccctac
     4741 taaattgaaa aaccatccgc ttatcgaacc ttttgaattg ctgactgaaa tgtacagtct
     4801 gccaaaatat gaagaagtag atccaacgcc ttggatgaca ccattttatc ttgtcttctt
     4861 cgggatgatg gtcgcagatg ttggttacgg attgctgatg ctcattggga caatactagc
     4921 tcaaaaacta tttgttttac cccgcgggat gaaacgtttc gtaaaattct ttgagattct
     4981 ctcggtacca tcgatcatct ggggattgat ctacagttca tttttcggac aggcattacc
     5041 aaaagaacta tttggtatcc gcttgccttt cccaatccta tctacgacgg atgacgtcaa
     5101 tacgattttg attttatccg tgattttcgg tctgatacag atcttggttg gattattcgt
     5161 ggcagcaaag gaaaatatca ggaggaaaga ttacttgagt gctgtcagtg atggttttgc
     5221 ctggcaaggg atcctgattg ggatcgttat tgcattgatt ggtgtcatgt tgataaaaaa
     5281 tacagcattt gtttatcttg gcgcggggct atcgatcatt gctgctttat gtatcttaat
     5341 cgtgccgatt atccaatcgc cgtctaaagt caagggtgct gccaaaggag cgtataatct
     5401 ttacggattg actggttata tcggtgattt agtcagttat acacgtttga tggcactagg
     5461 tatttcaggc ggaagtattg gcgcggcatt caatatgcta gttgctttca tgccgccagc
     5521 cgctcgattc agtgtaggga tccttttgat catcgcttta cacgcactga atatgttttt
     5581 gacactgtta agtgcctatg tccatggtgc acgattgcag tatgtcgaat tttttggaaa
     5641 attttataca ggaggcggac gtgcctttga cccgctgaaa acggcggaaa aatacgtcaa
     5701 catcaatcat aagaaaaaaa acaataaata attggaggat ttttatacaa tgatggatta
     5761 cttaattagt caaaatggtg gaatggtatt tgcagtatta gcgatggcaa cagcgacgat
     5821 tttttcaggg attggttcag ctaaaggtgt tggaatgaca ggtgaggcag cagcagcgtt
     5881 aacaacaagt caacccgaaa aattcggaca ggctttgatt ctgcaattgc ttccaggtac
     5941 gcaagggttg tatggattcg ttatcgcctt tttgattttt atcaacttag gaaatgatat
     6001 gtcagttgta caaggattga actttctagg cgcttctctg ccgattgctt ttacaggttt
     6061 gttttcaggt atcgcccaag ggaaagttgc agcagcaggt atccaaattt tagcgaaaaa
     6121 acctgaacat gctacaaaag gaatcatttt tgctgcgatg gttgaaacat atgcgatttt
     6181 aggtttcgtt atttccttcc tattagtatt gaatgcctaa gcataaagga ggaaaagcag
     6241 atgaacgcca ttgaaaaaat catctctcag atgaatgaag cagcagagca agaaagagcg
     6301 gcgttagagc aagaagagcg aatgaagatc gatcaaaact ttgaacaaaa acgtacgcaa
     6361 atcgaaacag aacatcaaaa acaaaaagaa aaacaaatcg aattacttga aaaaaaatac
     6421 cgtcagctta ggaatcgcca acaagtagaa gtacgacaag aaaatttgaa tgcgaagcag
     6481 gaatttttac gacgattgtt tgcagatgca atagctgaaa tggagaattg ggatgaatct
     6541 gaacaaatcc agtttatcaa aaattcattg tattcattac ctttgacagg caaagtaaca
     6601 ttcattgctg gagaaaaatc agcagcttat ctttctcaga cattgctgga tgaatggaac
     6661 aatgaacttc cctttatgat ggttttaagt gatgaaactg ttgctgatca agcgggtttt
     6721 ctgataaacg atcaaggagt tcagtataat tttcttttca gcagccttgt acaagatatc
     6781 caaggaacga tgagttttga gattgccaat caattattcg aataaggagg gatcagtgta
     6841 tggaatacca cgaactaaat cccttgattc gcggaagaga gcttgaactt ttgtccaatg
     6901 agacatttga acggatgatt caaacaaatt ctgtcgaagc acttggtgaa atattgaaat
     6961 ccacgattta cagtccatat atttatgacg gatatgaata tgacttcgaa cagaatcttt
     7021 taaaggagca gagccgtcta tttcgctggt taaaggaatc agcaccggag cctgaaatcg
     7081 tttggatcta tacgatgcgc tatactttcc ataatctgaa ggttttgaca aaagccgaga
     7141 tgacaaatca aaatctggac catttgtata tcgacgatgg attttattca ttagaaacct
     7201 tgaaggatgc cattcatacg caagtttcaa atgaactccc tgaaagcatt atggacagta
     7261 tacgagaagt acgtgattat tttgaagagt ctaccattct tcagggaatt gacgtgattt
     7321 atgatcgaca gtttttgaca gagcagcgac ggcttgccga taagctagga tatcaagaat
     7381 tgctgacaga agtcattgct tttattgact tgaccaatat cacaacgatg gctcgaggaa
     7441 tattgcagaa aagaacatat gggttcatga cgacggtact ttccagctcg ggaagtatcg
     7501 aaaaagaagt atttttgaac tttgtagata aagatatgga agattacacg aattttctat
     7561 tagagacaga ttatgctgat tttctgcgcc ctgtcatccg tgagggaaat cttgatctcg
     7621 taaagcttga acagttgaaa gatgattatc taagttcatt gtatcaagaa gcacaaaccc
     7681 aggctttcgg accactgcca ctactagcct ttttgaacgc aaaagaggta gaaagcaaaa
     7741 accttcgttt actggttatc ggcaaaagaa gccatttcga accagaaaca atcagaaagc
     7801 gggtgagaca agtctatgac gcataagatc ggtgtagttg gcgacaaaga ttctgttttg
     7861 ccattcaaat tgtttggctt tgatgttcgt tatggtacaa ccaagcaaac cgttcgtcag
     7921 gcaattgaag aaatggcaaa gaatgagtat ggcgtgattt atgtcacaga gcaatgtgca
     7981 gcattgagcc cagaaacgat cgaacgttac aaggaacaaa tgatacctgc tatcgtattg
     8041 attcccaatc atcaaggatc attagggata gggctggaag aaattcaaaa aaacgtagaa
     8101 aaagcagtag gtcaaaatat cttatagtat gaaaaggaga tagaaatttt gcaaattgga
     8161 aaaatcataa aagtctccgg acctcttgta atggctgaga acatgtctga tgccagtatc
     8221 caagacatgt gtctagtggg agatctagga gttatcggag aaattatcga gatgcgtggc
     8281 gatgtggcgt ctattcaagt atatgaagag acttctggaa ttggtccagg cgaaccggtt
     8341 cgctctactg gtgaagcttt atctgttgaa ttaggaccag ggatcatttc acaaatgttt
     8401 gacggaatcc aacggccatt agataccttt atggaaatca cacaaagtaa tttcttaggg
     8461 cgcggagttc aattgcccgc attggaccat gagaaaaaat ggtggttcga accaacagta
     8521 gaagccggag aaacggtgag tgctggagat gttatcggga tcgtagaaga aacaaaagtt
     8581 ataaaacata aaatcatggt gccaaatggg ataaaaggaa cgatcaaaac aatcgaaaaa
     8641 ggttcattca ctattgatga aacagtatgt gtcattgaaa ccgaaaatgg tgacaaaaat
     8701 ttgacgatgc ttcaaaaatg gccagtaaga cgtagtcggc caatcaaaga aaaattaaac
     8761 ccggatgcac ccatgatcac tggacagcga gtgattgata cattcttccc agtgacaaaa
     8821 ggaggagcag cagctgtacc tggtcctttt ggtgcaggga aaacagtcgt tcagcatcaa
     8881 attgcaaaat gggccgatgt ggacatggtt gtctatgtgg gatgtggcga gcgtggaaat
     8941 gagatgacgg atgttttaaa tgaattccct gaattgattg accctaatac aggagaatcg
     9001 ttgatggaac gtacggtatt gattgctaat acatcaaaca tgccagtagc agctcgagaa
     9061 gcttctatct atacgggaat cacaattgcg gaatatttcc gtgacatggg ttatgacgtt
     9121 gctatcatgg cagactccac ttcacgatgg gcagaagcac ttcgtgagat gagcggacgt
     9181 ttggaagaaa tgcctggtga tgaaggttat ccagcttatc ttggttcacg gcttgcggaa
     9241 tactatgaac gatctggaag agtcattgca ttaggatcag aaggtcgcga aggaagtata
     9301 accgcaatca gtgctgtatc cccgtctgga ggggatattt cggaaccagt cacacaaaat
     9361 acattgcggg tcgtaaaagt attctggggt cttgattcca gtttagccca aaaaagacat
     9421 tttccttcga tcgattggat acaaagctat tctctttatt caacagaagt agggaagtat
     9481 atggatcata tccttcaagc tgactgggca tccatggtca ccgagggcat gcgtattttg
     9541 caggaagaac agcagctgaa tgaaatcgtg cgtttggtag ggattgattc cctttcagat
     9601 aatgaccgtc tgacattaga agtggcaaaa tccattcgtg aagattattt gcagcaaaat
     9661 gcttttgatg atgtcgatac attcacttca agagagaaac aattcaaaat gttgaagatg
     9721 atcttgacct ttggtgaaga aggacgccgt gcgttgtcgt taggtgctta tttaagtgag
     9781 atcatggacg gtaccgtatc gattcgtgaa aggatcggac gaagcaaata tatcccggaa
     9841 acagaattag aaaaactaga tcaaattatt ggtgaaatca agcaaacgat gcaaacgatc
     9901 gtttcagaag gaggcatgag caatgattaa agaatatcgt acgatcggtg aagtcgtcgg
     9961 tccattgatg gcggttgaaa aagttgctgg tgtcaaatat gaagaattaa tcgaagtccg
    10021 tatgcaaaat ggcgagattc gtcgtggaca agtattggaa gtccaagaag ataaagcaat
    10081 ggtccaaata ttcgaaggaa caagtggtat caatttacgt gactcttctg tacgattttt
    10141 aggccatcct ttagaactag gtgtatcgga agacatgatc ggtcgagtat tcgatggttt
    10201 gggacgaccg aaagataacg gaccagagat cctgcctgaa aagcatttgg atattaacgg
    10261 agaagtaatc aatccagtag ctcgggatta tccagatgag tttatccaaa caggtatctc
    10321 tgcaatcgat catttgaata cattagttcg tgggcaaaaa ttaccagtat tttctggctc
    10381 tggtcttcct cataaagaat tagctgctca gatcgcgcga caagcaacgg tactcgattc
    10441 atcagatgat ttcgccgtag tctttgctgc tattggaatc acttttgaag aagctgaatt
    10501 tttcatggaa gattttcgtc aaacgggcgc aatcgaccgt tctgtcatgt ttatgaattt
    10561 agcaaacgac cctgcaatcg aacgtatcgc tactcctcgt atggctttga ctgctgcaga
    10621 atatcttgct tatgaaaagg gaatgcatgt tttggttatc atgacagaca tgaccaacta
    10681 tgcagaggca ttgcgagaga tttctgcagc acggcgagaa gttccaggaa gaagaggata
    10741 tcctgggtat ctttacacga acttagctac tttattcgaa cgagcaggaa gaatccgcgg
    10801 actaaaagga tcggttactc aaattccgat tctaacaatg ccagaagacg ataaaactca
    10861 tccaatccct gacttgactg gttatattac agaaggacag atcatcttga ctagagagct
    10921 ttacaagagt gggattcagc caccaattga tgtactcccg tctctttcgc ggttaaaaga
    10981 taagggaact ggacccggaa agacaagaga agaccatgca gctacgatga atcagctttt
    11041 tgcagcctat gcacaaggaa aacaggcaaa agaattagcc gtagttttag gtgaatctgc
    11101 tttatctgat gtagacaaga tctatgcgaa gtttgctgag cggtttgaaa aagaatatgt
    11161 caatcaagga ttctacacga accgttcgat tactgaaaca cttgatctag ggtgggaact
    11221 gctatcgatg ttgccaagaa ccgaattaaa acgaatcaaa gacgacatgc tggacaaata
    11281 tctgccagaa gggaagtgag ttctgatggc acgattgaat gtgaatccga cccgcatgga
    11341 attgactcga ttgaaaaaac agttgacaac tgccacaaga gggcataaat tattaaaaga
    11401 taagcaagat gaactgatgc gtcaattcat cctgttgatt cgaaagaaca accagctgcg
    11461 tcaagaaatg gaagagtcta tgacagaagc aatgtctgat tttgtattgg caaatgcttc
    11521 gatcacggaa cctttcatcg aagaattatt tgttttgccg gcggaaaacg ttgatttgtc
    11581 tatcgtagac aaaaatatca tgagtgtaaa agtacctgtg atgaattttg aatatgatga
    11641 acagctgaca gacacacctt tggaatacgg gtatttgaat tccagtgccg aactggatcg
    11701 gtcaatcgat cggtttacta ccctgcttcc aaaacttttg gaactgacag aaatcgaaaa
    11761 aacttgtcaa ctgatggcga gtgaaatcga aaagacacgt cggcgagtca atgcattaga
    11821 atatatgacg atccctcaat tagaagaaac gatctattat attcgtatga agctggaaga
    11881 aaacgagcgc gcagaagtaa caaggttgat caaagtaaaa aatatgggta ccactgacta
    11941 agtggtatct ttttttaaaa gaggaaaacg atgattaaac aaagcaaaca aataaagaaa
    12001 cgtttgccgg ctgttcagct tctcgcatta ggattttttc tcctgatttt agttggcggc
    12061 ggcctactta ccctaccatt ttttagtaat agtgggcatg ggacaaattt tatcgatgcg
    12121 cttttcactg caacttctgc tgtctgtgta acgggattga caacattgaa tacagcagag
    12181 cattggaaca gtgctggtca atttttgatc atgcttttga ttgagatcgg cggtcttggt
    12241 tttatgatgg ttccgattct atgctttgca ttgataaaga agaaagtcag tctcagcaca
    12301 cgtattatat taaaagaagc gctgaatcta gaagaagtat caggtgtgat gaagctcatg
    12361 ctttacatcc taaaattggc ctttgttatc caattgattg gtacggttgc tttatctttt
    12421 gtatttgtgc ctgaatttgg ctggttgaaa ggtatctggt acagtttgtt ccatgcaatc
    12481 tctagttttt gtaatgcagg gtttgatttg ttaggagaca gtttagccaa tcaccagcaa
    12541 aatgtttatt tgatcatggt ggtatctgct ttgatcattt ctggtgggtt aggcttcatc
    12601 gtatggcgtg atttactgga atatcatcag aaaaagaaga tgacgattca ttcaaaaatc
    12661 gcattaaccg ttacaggtac ccttttatta ggcggattcg ttgtgttctt tttcacagag
    12721 cgcaatgggg caacgctagt ggaagggaat atgtttcaac gattggcaaa cacctttttt
    12781 atgagtgtga cacctagaac ggctggatat tattcagttg attacctcca tatgagccat
    12841 gcaggtctga ttttaacgat gtttttgatg tacatcggcg gaacatctgg gtcgacggcc
    12901 ggcggattga aaacaactac gttaggtgtg ttgctgatcc agatgcgttc gatgttgaaa
    12961 ggacgtacga gagcggaagc atttggccgt accatccgac aaggcgcagt gttgcgggca
    13021 cttactttat tttttgttac gttgacgttg tgtgtagtcg ccatcatgat tttatcggtg
    13081 actgaaacga tacctaaaac ttctggcatc gaatatattg cttttgaggt cttctctgcg
    13141 tttggtacag taggtcttac gatgggattg acgccagact tgactgcaat agggaaattg
    13201 atcattatag cgcttatgta cattggacga gttggtgtat tgactgtggt cttctctctg
    13261 atgacaaaag cacaaagaac acaatcaaaa tacaaatatc cagaagaaag tgtcatgatt
    13321 ggataatttc atcttctgac agaaacctag tgatttagta aaataagaaa ataaaataat
    13381 ttttcttgct tcttggatac tttaagtgca aaatgttcgt ttattttttg ag
//
LOCUS       DAIGOK010000077        13399 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.077, whole genome shotgun sequence.
ACCESSION   DAIGOK010000077 DAIGOK010000000
VERSION     DAIGOK010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.077"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(59..601)
                     /locus_tag="NOA01_001802"
     CDS             complement(59..601)
                     /locus_tag="NOA01_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HCI0632989.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            736..1125
                     /locus_tag="NOA01_001803"
     CDS             736..1125
                     /locus_tag="NOA01_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632990.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            1129..1893
                     /locus_tag="NOA01_001804"
     CDS             1129..1893
                     /locus_tag="NOA01_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCI0632991.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            2022..2969
                     /locus_tag="NOA01_001805"
     CDS             2022..2969
                     /locus_tag="NOA01_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCI0632992.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            3099..3839
                     /locus_tag="NOA01_001806"
     CDS             3099..3839
                     /locus_tag="NOA01_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632993.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(4130..4798)
                     /locus_tag="NOA01_001807"
     CDS             complement(4130..4798)
                     /locus_tag="NOA01_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCI0632994.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            4932..5219
                     /locus_tag="NOA01_001808"
     CDS             4932..5219
                     /locus_tag="NOA01_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0632995.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            5635..7173
                     /locus_tag="NOA01_001809"
     CDS             5635..7173
                     /locus_tag="NOA01_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HCI0632996.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            7170..8135
                     /locus_tag="NOA01_001810"
     CDS             7170..8135
                     /locus_tag="NOA01_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0632997.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            8122..9444
                     /locus_tag="NOA01_001811"
     CDS             8122..9444
                     /locus_tag="NOA01_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCI0632998.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      9507..9611
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            9685..10428
                     /locus_tag="NOA01_001812"
     CDS             9685..10428
                     /locus_tag="NOA01_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI0632999.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            10443..11435
                     /locus_tag="NOA01_001813"
     CDS             10443..11435
                     /locus_tag="NOA01_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCI0633000.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            11447..12184
                     /locus_tag="NOA01_001814"
     CDS             11447..12184
                     /locus_tag="NOA01_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0633001.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            12174..12896
                     /locus_tag="NOA01_001815"
     CDS             12174..12896
                     /locus_tag="NOA01_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCI0633002.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(12980..>13399)
                     /locus_tag="NOA01_001816"
     CDS             complement(12980..>13399)
                     /locus_tag="NOA01_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HCI0633003.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         4412 a         2295 c         2786 g         3906 t
ORIGIN      
        1 aaaactgtct aacatttaaa atttaattta gatattagac agtttttaag tttgtgagtt
       61 attttccttg tcgttttctc acttttttag tccagaaacc acctgaaata tattttggct
      121 cataagaaat gataaaagca cgctcttcga tttctttgat caggcgatac agttcacgtt
      181 cgctttttct aggagaaagt atttcgagaa ctagccgctc accttccagc cccattgcag
      241 aagtctgtgt cacaccataa ccgtgttctc tcagtatacc cggcaaactt ttatcttctg
      301 cgctatttgc tggcaaaatc gttgtgacca tgatgtatcc taatgccaat ttgtcttcaa
      361 tcaaaattcc gacactgata cctactgcgt aacctaacgc atagacaaat aaattcagtg
      421 gattatctaa acgatctaaa acaagggata agccgacgat ataaatcgtg atctcgacca
      481 tactgacaag tggagcaatc agtcgatagc ctttcattgt caacatgaaa cgtaacgtgt
      541 tcagagtgat ataagcaaaa ttaatgagga agatcatccc caacattttt ggatcaatca
      601 tttgtaaaac tcctttcatt tatataacaa caatatcaaa aaaaatcgta agtgaagaaa
      661 aattgaaact ttatacatac aaaaaaatga taaaatgatt ataatagaat tataaagata
      721 ggttaggtga ttaaaatgaa aacggtcaat cgtgtgtttt gtgccagcca cgtgctaagt
      781 ctccttctca gtatatactt aggtgtctat tcgattcaac agccaattga ggtagagaac
      841 attgtttttc agatgtcgct gacagatggt ttaattttaa gcgttttttt ctttttgata
      901 ctgtttattg gtaatatttt cggtagcgtt gccagttttt tgaatttttc tatttatcca
      961 ctcctttcgc tagcattagg agtggtggga ctttgttctc tgtgcttatt tcccgagcca
     1021 ttcagtcctg catttatatt atttggtaca ctgaacctgt tccagacaac tgttggagca
     1081 tggttgctat ggcgatcagg aaatttaatg aaaataggtg agtaacaatt gaatccaatt
     1141 atattgaaac gttctgtcca tcatattcca gtgttagaag ttgttctgaa agaattaaga
     1201 gaggaacgta ttcccgttgt catctattat cacggatggc agacaagtaa ggagttagtt
     1261 ttgacgcaag gaagaaaaat tgcgcagcag ggcattcgtg tgatcctgcc agatgcgatg
     1321 aatcatgggg agcgcaaaca gccggtttct tctattccat cctatacatt ttgggatagt
     1381 atctacggaa atctgtttga atttgacaca cttatcaagc atttagaaaa aagaggtctt
     1441 ctacatgatc agcttgcagt tggcggggtt tcgatggggg gaatgacaac atgcgccttg
     1501 ttggctaaac atcctcatat agtagcgggc gtgtgtttga tgggttcccc tgcacctttg
     1561 gaatatggaa aagaaatcgt aaggcgcgca tcggaatata attatgtcgt accaaaagac
     1621 tattttgatt tgatttcatg ggttaagagc tatgatttgt cattgcagcc ggaaaagtta
     1681 gctggacgtc ctctgttctt ttggcatgga caagctgacg aaaaaatccc ttatatacaa
     1741 acggaacgat ttgtagaaaa aaatcggcat aagtcttacg gaaaagaaat tgtttttcgt
     1801 tcctcaaaaa cacagcgaca tatggtacaa ataccactaa tggaagaagc ttccactttt
     1861 ttagcaaaaa atttgataaa aaagtataaa taattgaaac ggaaaatcac tcatgacgat
     1921 agaggatttt ccgtttttta ttttgggcta taataaactc tgttaggaaa atttggtata
     1981 attgatagta agaatgacaa catgttagaa aggaagagag catgcccact ttagcagatg
     2041 ttgcaaaacg tgccaatgtt tcaaaaatga cggtctctcg agtcatcaat catccggaac
     2101 aggtgactga cgaattgaaa gagttagttt tctcagcaat ggctgaactg gattaccgtc
     2161 caaatattgc tgcaaaagca cttgtcacga atcggtcgca gatcatcaaa ttatttatac
     2221 tggaagaaat cgacacaact gagccctatt acatgaattt gctgatggga atcgctaaac
     2281 ggatcggtaa agcgcattat tctctccaat tagttacaaa tgatgccttc gacgtcggtt
     2341 cttgtgatgg atacatcatc acaggtgtca gaaaaaaaga cttcgattgg atcgaccgtt
     2401 tggaaaaacc agttgtcttg tttggggaaa attctgaagg atatgattat gtagacagcg
     2461 ataatgaata tggtacagca aaagcaacgc aatatgcctt agaaagagat tatcaaacga
     2521 ttatttatat cggtatcgaa gtggacgagc cttttgaaat ttcaagggaa actggttatt
     2581 tatctgtgat gaaggaagtt gatcttccta gtaaaatctt gcggtttcca aatcattcga
     2641 cagaagtaga aagatatatg acagaaaact gggcagattt tacagataaa acttgttttg
     2701 tatgtagttc agatcggcta gcactaggag cagtaagagg gattataaat gctggaggac
     2761 acttgcccga agaatttggt gtgattggat ttgatggggt atttttagac caaatcagtt
     2821 cacctaaatt gacaacgatc aagcaagata ttatacgtat gggtgaagta tgtggagaaa
     2881 tgttgttaaa aaaaatagaa gaaaaagggg cgagtcaagg ttatcgtcgt ttttttcctg
     2941 aactagttat cagagagaca acgaattaag tcttattact atatatatat ttatgaaatt
     3001 catcgctatt ttcttaaaat aatcagcgca ttgctactga aaacaatgaa tctgtgctat
     3061 tattgagaaa agttccattt ttgagaagga tggtttgaat gaaagaaatc agacgaaaac
     3121 atttccgttt tcctgcgtac gatgatgagt taggcgtaaa attaaccaac gacaagaagc
     3181 gagtattatt tgacggacaa gaagatcttt tgactgaaca gaatcagtcc ccgtttgaac
     3241 agatgaaaga atttcatttc acgaacacga acaccgagaa aatccgtcgc aagcgtcgtg
     3301 caacaaatgg attgaagtca gctccgcgta aggaaaattt ggaaaagcat aaaggaaaat
     3361 taccggatta taccggccat taccaatcag aaatcacatc gacaggacga aaaaaacttt
     3421 ttggtgattc ctctcgttct gtcagacaaa gtgaaacaag gacagagtcc aagcatacta
     3481 cacgaaacgc atctgttgac acgaaggtgc gggagcgttc ttattttgtt cctaaatatg
     3541 tacccgcatc gattatccca gaccctagag aaagcaaaat ttctgaggat catttaatcg
     3601 aatcgatgaa aaaaacggat gactcttatc tgttgttcga tacagaaccg acgccgtatc
     3661 aagaaaaaag aaatggtgag ccgtctgtac gaacattcaa tcgaacaaaa acgttagagg
     3721 aaacaaagca gtcagaagaa aaaaaatcga aaaaaagcca tggtatctta gaacgctcat
     3781 tacaaggatt gatcgatgat caaggaaact ctttgggaga aaatagctat tttaaataag
     3841 tgatatacaa atttatattc ggctacagtt ggaaaggaaa cgctgcggcg tttccttatt
     3901 ttatactcta tccgaaagtt tatatcgtat cctcaaattt ttttgagttt aagcgaattt
     3961 tttctcccaa taagtaagaa aataatggca aaataaaaaa acaaactatt ttcgcagatc
     4021 gtttgcaaaa atagtttatt tttttataaa gaagatttta ctgaataata tcattttagt
     4081 ttacataatg atatttctgt aaattaaata aagtagtgaa aaataagaat cagtgctcta
     4141 actgttcaag aggatcaagt ggttcaaatg tttctgtagt ctgttttgct tccaattggg
     4201 gcaaaacaga tccaatttcc agatacttca ctttattcaa tgtataagat agaatagaac
     4261 agctaagtaa tgtatacaga aaatgtgtga gatccaaata aagcaaggag acaactaaaa
     4321 taccaaaagt aatcaagaaa agacttttgc caatcttaat atgaaaatat ttttcaatgg
     4381 cttgataaat gatatctgtt ccgcctgtgc ttccgccgaa tcgaaagacg agaccgcaac
     4441 caatgccaga aagaatacct gtcatcaaac tattgatcag tagactatct acagcaaaat
     4501 gtatatgaag gctctcccat aaatgtaaaa agaaaactag cgcaaatgtt ccgacgatcg
     4561 tccggagaaa agcgtgtttt ccaaaaagaa acaatgagat aagcaataaa ggtaaattaa
     4621 aaagcagtga agtgacagaa ggagcgaatc caaaaagatt atgcaaaagc aaagcaatac
     4681 cagtaacccc accatctgcc agcttagatg gaatattgat tcctgtcaca gctaatccgt
     4741 agattcctgc gcctaaaatg attccgcaca attctgtaaa taaatttaat tttttcattt
     4801 ctagcctctt ttcttttgaa aattttcagc aacttcttta ttatactgac ttatactctt
     4861 acttcaagag gaaaatgaaa aaaaattatt tttggctgtt tctttttatt tatcaaataa
     4921 ggagtggaaa aatgcgagca gcagacttat tgacaattct tacaaaatca cccaaaaaaa
     4981 cagagtatca aggagttttt ttggctcaag aacaaaaact ggtgcctgtg acaatggtcc
     5041 agataaataa agcaaatcaa tttatctttt tatttgaaca cggcaaagca ccacttgcta
     5101 tgaaagacat ctttcttttt ttgatgaaga accgtgagaa agctgtttat tatcagtcaa
     5161 aagaagaact cattcctctt tatggagtga aagagataga aaataaagtg gtgatataag
     5221 taaaaataag aaaacaagac gtttatgatg tcaaaacaac cattttttac atttttggca
     5281 aaaaagtgtc atgatacagt ataatattcg ttgaacaggc caataaaggt gttctccccc
     5341 acgagatttc tactttattg gctgtttctt tttattaaaa taaaagttgt gtgtgtaaaa
     5401 aagatatagc tgattccaag tgacaatttc gtatagattt ttatggtatt cgtagtgaaa
     5461 aaacgagaac caaaaagttg atccgacttt gttgcttgga ttcttttttt gtcagcatat
     5521 ttagaggtat ttttttcttt tttttgttcg tgctgtaagg ttaaggcaag acaagttagt
     5581 tgggaaaaag gaaagaaatg tttatactta aagaataaca gaaaggaagg tcgcatgaat
     5641 aattttatac aaacattaag tgaaagaaaa ggcgagttgc tggaagctgc atttcaacat
     5701 ctgtccattt ctctcatttc tttgctgatt gcagcactga ttgctattcc tttagcgatt
     5761 tgggcagcca atcataaaaa aatggcagaa gtcttattgc agatcacggg tgtgctccaa
     5821 acgattcctt cacttgcctt attaggttta cttatcccat ttgtagggat cggaactgtt
     5881 ccagcattga ttgcacttgt tatttacgct ttgcttccga ttttccaaaa cacgtatatt
     5941 ggtctagctg aaattgaccc gtctattgaa gaagcagcgg tagcatttgg tatgtccaga
     6001 atgagacgct tgctgaaagt agagcttccc atcgcattac ctgtcattat ctcaggtatc
     6061 cgaactgcct tagttttaat catcggtacg gctactttgg cagcattgat cggagcaggt
     6121 ggattaggga catttatttt gttaggtatt gaccgaaata cacctgtttt gacattgatt
     6181 ggtgcgatca gttctgcctt gcttgcaatt atattcagcg gactgattcg ctggttgcag
     6241 cataagaaac ctcgatattc catcattact ttagcagcag tggtgtgttt atttggcggc
     6301 gtttctttat atcagtctgc cttgtttaaa aatgaaacca tcacgattgc tggaaaatta
     6361 ggagcggaac ctgaaatcct gatccaaatg tataaagaat tgattgaaga caacacaaaa
     6421 acagaagttg aattaaaacc gaattttggc aagacgagtt ttttgttcaa tgcactggat
     6481 caaggtcaaa tcgatatcta tcctgagttc acaggtactg tcctagaaag cttggtgaag
     6541 gtacctgaag caatgaaaag tgctgatttg acaaaagaaa aaacatatga cgaagcagaa
     6601 gaattattga ataagcaatt cgatatgaca ttattaaaac caatggctta ccaaaatacg
     6661 tatgccttgg cagtcaagag atcttttgcg aacgaacatc aacttaaaaa gatttccgat
     6721 ttggcagcta tctctgatca gatcaaagcg ggattcacac tggaatttat tgatcgttca
     6781 gatggatata aaggaatcca agaagcatat ggcttgaatt tttcaagtgt acaaagcatg
     6841 gagccaagtt tgcgttacca agcaatcaat aatggagatg tcaatattgt ggatgcctat
     6901 tccacagata gccagctcaa ggagtacgat ctagtgactc tagaagatga tcgagggtta
     6961 ttcccagctt atcaaggcgc accgctgatg aaaaaagatt ttgctaaaga acatcctgaa
     7021 atcgttaaag cactgaataa attaagcgga aaaataacag aggatcaaat gattgatatg
     7081 aattaccaag tcaatgtcga aaagaaaaag ccgacagaag tagcacgaat cttcctgaaa
     7141 aatgaaggat tagtaaagga ggaagatcag tgactaaatt aatcgagttc aaacatgtgc
     7201 aaaaaaagta tgatggaaag tatgtcatcg atgacctaaa tctggcaatc aaaaaaggag
     7261 aaatctttgt tttggttggt ccttccggta gtggaaaaac aactactttg aaaatgatca
     7321 atggattatc taaaccatca gcaggagaca tctacttcaa aggaaaatcg ttaaatgaat
     7381 ataatctgca gaaaatgcgg tggaatatgg gatatgtgct gcagcaaatc gctttgttcc
     7441 cgaccatgac agtcaagcaa aatattgaag tcatccctga aatgttaggt tgggaaaagc
     7501 agaaaagagc agaccgtgta gatgaacttc ttcaaaaagt tggcttatct cctgatattt
     7561 atcgagatcg tatgcctaga gagttgtctg gaggagaaca gcaacggatc gggatcatcc
     7621 gagctattgc tgcaagccca gatgtgattt tgatggacga gccatttagc gcattagatc
     7681 cgatttcaag aaactcatta caagaattgg ttttgtcctt acacgaagaa ttgggcacga
     7741 ccatcgtttt cgttacgcac aatatggaag aagcaatcaa gctaggtgat cgtattgcat
     7801 ttatgaagga tggagaaatt atccaatgtg acacaccaga gcagttattg atgaatccta
     7861 aaaacgacta cgttcggcat ttctttgatg aaccgaaaca gacgaaagag tggcgagtag
     7921 aagatttagt cgtcaatgga tatttcttaa atgaaatacc agaaaatgct cgtcaacaag
     7981 tttgctttga tacgcctatg aaagaagtat attcgcttct ttcggtgtat ccaagcattg
     8041 tgatagtaga aaagcaaaga agtattgggt cgctgacaag taaagaaatc tttgcttttt
     8101 taagtcgaga ggaggaagga aatgaaaaca tatgatgcaa tcatcatcgg ttcaggtgtg
     8161 agcggcttaa gtgctgctta tggattgaaa gaagcaggta aaacagtact agtggtcgaa
     8221 gaagatctat ggggcgggac ttgtcctaac cgtggatgtg atcctaaaaa agtcttgtta
     8281 agtgcagtag aggcacgtaa tcgggtaaaa caattgagtg gaaaaggttt taacgagatc
     8341 ccaactgcca actgggaaga acttcaaaaa ttcaaacgaa catttacgga tccagtccca
     8401 gaaagccgaa aaaaacaatt agcagaagct gaaatcgatc atctttcagg aaccgctcgt
     8461 tttttggatg attcatctat tgaagtcaac gaagaagtct tccatgcgga ttacttagtt
     8521 ttggcaaccg ggcaaagacc tacgatctta ccagtggagg gaaaggaata cttgaaaact
     8581 agtgcagact ttttatcatt gcctgtactg cctaaagaaa tcatttttat cggtggtggt
     8641 tatattgctt ttgagctagc tacgattgcc aatgctgcag ggagcaaagt aaccattgtc
     8701 catcataatc aacggccatt aaaagaattt gaggcgtctt tggtagagga agccgtccat
     8761 caaatggaag catctggcat tcagtttgct tttggtgttg aaacccaaaa gatcatttca
     8821 gaaggtactc gctatcgttt agtcggaaaa gagacggaat tagtagcaga tatgattttt
     8881 tgtgcgactg ggagacagcc gaatacagaa tctttagcac ttgagcaagc gaatatcgtc
     8941 tttgataaac acggaattgc agtcaatgat tatctgcaaa cgagcaatcc aaaaattttt
     9001 gcgtgtggag atattgtttc aaggaaaacc ccaaaattga caccagtcgc cacttttgaa
     9061 ggaaattatg tagctaagcg gataacggat gcaacaagcg aacctatcaa atatccgatt
     9121 atacctacga tcgtctacgc cagtcctaaa cttgctgaag taggagttac aaaaagtcac
     9181 gcctcttcgt cagatcaagt ggtagagatg gatcttacca gttggttcac ctatcatcgt
     9241 gtcaatgaac cagttgctaa ggctgaattg acctttgacc agcaaaatta tttgattggt
     9301 gctgcagtga ttagcgagca agcagacgaa ttgatcgatg atttgacact cgtcatcaat
     9361 caaaaattga ctaaaaaaga gttggatagc tatatcatgg ggtatccgac attggcaagt
     9421 gatctctcct atttattgaa ataatcagca tgataactaa agcgcttgtt gctttttcct
     9481 tcttgtgcta cactttaaaa aatgaatacc tgtttctggg gatgtcgtag acgacatgag
     9541 agtaagttgt gaacttagac ccattgaacc tgtcagttaa tactggtgaa gggagaaaca
     9601 tagcagacgc ttgaagcggg ttactatgtc gtgcccattt caagcgtttt ttgttattca
     9661 tattagaaga aaggaggtaa caaaatgaag aaaaatcttt atccagcagt cagtatggca
     9721 ttcattttaa ttatttggca gattgttgtc atagtttgga aaatacctgc ctttatttta
     9781 cccggacctg ctgctgtatt ttctgcatta gcggcagatg cacaagtatt actttcccat
     9841 tctgtcacga cattaaatga agcagtgctt ggtttggtaa tagctagcgt tctttctttt
     9901 attactgcct tggcaatgga ttattggaag tttttagaaa tgtcttttta tccgttacta
     9961 gtgatttcac aaatgttgcc aatcatggtg ttagggccat tacttaccct atggtttggt
    10021 ttcggtatgc ttccaaaagt gatccttgtt gtcttgataa gctattttcc gatcgtagtt
    10081 tctttttcgg atacattgag gaaagtatca aaagagcaaa tcatcttttt gaaaacgatg
    10141 ggggcagata cagggaagat ttatcgaata tataaaatac cagcgggttt atccggcttc
    10201 ttttccggtt taaaagtggc tgctacttat tgtgtcagcg gagcagtcgt tggagaatgg
    10261 ctaagcgctc aagcaggctt agggtattac atgatacgcg taaaaaacag ctaccagatc
    10321 gataaagttt ttgctgcgat catttgtgtc attctattga gtttattgct gaatggcgct
    10381 tgttcattat tgaaaaaagg gtattatcgt atgctttata ttttttaaga gaaggggatc
    10441 aaatgaaaaa attatggctt ttattaccgc tactgttgct tttatccgct tgtggcactg
    10501 caaaagaaac aagtagcaaa caagaaataa aggatttgaa aaaagtgaca cttgttttgg
    10561 attatgtacc gaatactaat catactggca tctacctagc aaaagaaaaa ggctattata
    10621 aagaggctgg tttaaatgtc caaatcatcg aaccaggaga taattcgaca agtattggtc
    10681 tagtaggtgc ggacaaagca caatttggtg taagttatca agaagatgtc acttatgctc
    10741 acgcagatgg acagaatata ccagtcaaag caattgctac agtgatcaaa cataatactt
    10801 ctggattcgc caccctatca gacagtaaca tccattcacc aaaagatttt gaaggaaaaa
    10861 catatgcagg ttggcaaagt ccaagtgaag aagctgtctt aaaagctgtg atggaaaaag
    10921 acggcgggga cttttcaaaa ctgacaatgg taggaagcaa tggagaaggg ccagaaagct
    10981 taggaaaatc ctcagatatc caatggtact ttgaaggttg ggatatgatc aaagcaaaag
    11041 aagcgggtat tgaactaaac tatatcccat tgaaagaatt agatgaacgg ttagattact
    11101 atactccggt aattattacg aatgatcaac tgatcaaaag tgatccagaa ttggtacagt
    11161 cttttatgga tgccacgaaa aaaggctatc aggaagctat taaggatcca aatgacagtg
    11221 ctaaactgtt gcaaaaatat gcaaaagaaa atgatcggac gtttttagaa gaatcacaag
    11281 cattcttgtc aaaaaattat acggatgatc ctgagaattg gggattgatg gaagaaaaag
    11341 tttggcgcaa ttatacagct tttatgcagg aaaacggatt gatcaagcaa gatgtacctt
    11401 cgcaagaact attcactaat cagtttataa aataggagtg atagatatgt caatccaaat
    11461 cagccacttg aatgtagtca ttgaaaacct gaaaatatta gaagacatcc atttcactat
    11521 ccctaaagat acctttacat cgattgtcgc gccaagcgga gccggtaagt caacattgct
    11581 gaaaacttta acaggagtcc agcctatgac ttctggaacg atcaaagtag acgatcaaaa
    11641 agtaactggt ctccatacag catttagtta tatgccgcaa gaagatatgt tacttccttg
    11701 gctgagcgtc tatcaaaacg ttacgttata tcaaaaaatc aatcatctaa caatcaatga
    11761 agaacaagta agggattatc tggatatctt tgggttaaac ggctacgaac attttttgcc
    11821 ggaacaatta tctggcggga tgaagcagcg aacggccttg ttacgaacaa tcatgaatcc
    11881 ttcttcttac ttgcttcttg atgaaccatt tggagcgtta gatgctatga ccagagggca
    11941 gatgcaagac tggatgttga agctgcctaa aaaagcaaag cggactactt tacttgtgac
    12001 gcatgatatc gaagaagcaa tttatttatc agacaggatt cttgtgcttt ccgcccgtcc
    12061 ggcacatgtg attgctgaga ttcaagtgcc tgaaaaacga cggtcacgtg aatggctact
    12121 gcagcaatca gagttgaagc aaacgattta tcagttattg gcaggtgaat tagatgttaa
    12181 gtgatgctca ttgtcattta gatgggagtc agagtttagc actccttcag caggaacaca
    12241 cgattttgac gatcatcaat tgtgattcgc cagaagaatg gaaggaaaac agacagcttg
    12301 cagcaagtaa gactcaagcc ttaagttacg gtatccatcc ttggaaagca gatagctata
    12361 cgtttgaaca agttgagcct tttttgaaaa aagcaaggat catcggtgaa atagggttag
    12421 ataatatttg gacgaatgta ccgatgacta cacaaaaaaa agtatttgag cgacagcttg
    12481 cttttgcagc cataaatgag aaacctgttg tactgcacac aaaaggatgc gaaaaagaga
    12541 tcttaaatta tatccaagct taccctaatc actatttgat ccattggtat tcttctttgg
    12601 actatcaaca agactatatt gatttaggct gttatttcag cattggtctt gatttgaaaa
    12661 agaatccagc agtctggcaa ctagctaaag cagtcccaat cgaccactta cttattgaaa
    12721 cggatggact acatgcagca gagtgggctt taggggggat tttttcagaa aaagattatg
    12781 tctcactgct tgaagaacag ttaaagacaa tcgctgagat caaagagcta tcagttgaag
    12841 aagttaaaaa aaatacttat caaaacttgg aacgtttcat tagattaggt aaataaaaaa
    12901 gagatgtgac acaactagtg tcgcacctct ttttggcttt ctttgtataa aagtaaactg
    12961 tttgattaat aaatgaatct tagtaccaac cgtttgcttc ccagaatgct ttcgccgctt
    13021 cccaagaacc ataacgtgaa gtaacatatt gttctgctac tctttcttgg tttgcagcag
    13081 agtagtcacc atttaagtaa gaagaatcta attggtaacg tccgatgtaa cgtccgttcg
    13141 ttgctgtgta agaaccgctt gattcttttt gtgcgatcca ttcttttgca gagcttgtgc
    13201 ttgctggagt agcagcagtt tctgttacag caggagcagg ttgtgtttca gcaacaggcg
    13261 ctgcttgttt ttccgtttga accgtttctt gtacaacagg ttgttcagct tggactggtg
    13321 cttcttgaac ggctgcttga ggttcagcag ctgcttgtgc agagccttct gttgggattg
    13381 ttaattgttg tcctgagta
//
LOCUS       DAIGOK010000078        13375 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.078, whole genome shotgun sequence.
ACCESSION   DAIGOK010000078 DAIGOK010000000
VERSION     DAIGOK010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.078"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(130..648)
                     /locus_tag="NOA01_001817"
     CDS             complement(130..648)
                     /locus_tag="NOA01_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCI0633004.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(650..1267)
                     /locus_tag="NOA01_001818"
     CDS             complement(650..1267)
                     /locus_tag="NOA01_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HCI0633005.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(1264..2118)
                     /locus_tag="NOA01_001819"
     CDS             complement(1264..2118)
                     /locus_tag="NOA01_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCI0633006.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(2115..3026)
                     /locus_tag="NOA01_001820"
     CDS             complement(2115..3026)
                     /locus_tag="NOA01_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCI0633007.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(3067..4401)
                     /locus_tag="NOA01_001821"
     CDS             complement(3067..4401)
                     /locus_tag="NOA01_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCI0633008.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            4609..5439
                     /locus_tag="NOA01_001822"
     CDS             4609..5439
                     /locus_tag="NOA01_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HCI0633009.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(5683..5965)
                     /locus_tag="NOA01_001823"
                     /pseudo
     CDS             complement(5683..5965)
                     /locus_tag="NOA01_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011066964.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6123..6317)
                     /locus_tag="NOA01_001824"
     CDS             complement(6123..6317)
                     /locus_tag="NOA01_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633010.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(6822..7007)
                     /locus_tag="NOA01_001825"
     CDS             complement(6822..7007)
                     /locus_tag="NOA01_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633011.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(7857..8198)
                     /locus_tag="NOA01_001826"
     CDS             complement(7857..8198)
                     /locus_tag="NOA01_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCI0633012.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(8198..8428)
                     /locus_tag="NOA01_001827"
     CDS             complement(8198..8428)
                     /locus_tag="NOA01_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCI0633013.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            8715..9053
                     /locus_tag="NOA01_001828"
     CDS             8715..9053
                     /locus_tag="NOA01_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633014.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(9258..9497)
                     /locus_tag="NOA01_001829"
     CDS             complement(9258..9497)
                     /locus_tag="NOA01_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HCI0633015.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(9473..9820)
                     /locus_tag="NOA01_001830"
     CDS             complement(9473..9820)
                     /locus_tag="NOA01_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HCI0633016.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(10552..11133)
                     /locus_tag="NOA01_001831"
     CDS             complement(10552..11133)
                     /locus_tag="NOA01_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCI0633017.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(11316..11942)
                     /locus_tag="NOA01_001832"
     CDS             complement(11316..11942)
                     /locus_tag="NOA01_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCI0633018.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(11964..13295)
                     /locus_tag="NOA01_001833"
     CDS             complement(11964..13295)
                     /locus_tag="NOA01_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HCI0633019.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
BASE COUNT         4179 a         2687 c         2215 g         4294 t
ORIGIN      
        1 ttataacgtt ctcatttaac aaaacttaag ttattaagac aaatgaataa acaactacgc
       61 tttcctcgaa acaaaaaaat aaagcacaaa tgcgcattta cgcactcatg ctttattcgt
      121 acaattaatt cagataacgg cagcgcatag gatgccaata acacttccta aaatagctcc
      181 tcctaaaatg tcagtaggat aatgtaaaag taaaaaaatc ctcgaataag cgatcaaaca
      241 agcaaataaa aaggctaata ttcctgcaac tggacttaat tcgaacaacg cagctactga
      301 taaaaaggct agcgctgtat gtcctgaagg aaaagaagaa gtcttcggtt tacttgtgta
      361 aaggacaagc cgacgatttt tttcataagg tcttttccgt cttgtcgttt ttttgataaa
      421 aagcaaacca acaaaaaaat agatcaaact gccgataaga atggctagac catattctct
      481 agtatcaggt atcaagacta aaacagcagc aaggagcaac caaataaaac caccattaga
      541 gatattactg ataaatttca aaaaattgat ttttcgacta tccgttttcc gttgcttggc
      601 gaacttgttg ataatactat catcgatttt agcaatcaaa cgcaacatgt tattcctcct
      661 ttttaagaac cttttcttgc gtacgatgga taaaaagcag gacttgaaaa taaaacaaga
      721 aactgaaacc taataaagca aaatagattg gtcctgcaaa ccagaatgcg acaagaagga
      781 tcaacgtgcc tgccaaatag aaaagcgcgc ttttgaaatc attaaaaaac aagaaaaatg
      841 cagtaatcca agaagcttct ttttgttcca gttgaagcaa gattttgtaa ctaaagagaa
      901 gcgcgcttaa tagaagcaaa aacaacgtag tatacatcaa tgtatagcca attcccccac
      961 cccaatagcg gaaaatctga taatcgatca atagaagaag aaaataaaca cttgctaaac
     1021 tatcttgctt tccataaaga gagaagttct cacgaaactt tttccagaat agtgaaccga
     1081 gctttttctc ttcatttttt ttgtaatgat aaatcatata cagaactgtt cgacttgcag
     1141 ttcctaatgt gaaaacaggc aagacgcaca ggaaccatag tagctgtaaa tggataagac
     1201 gtggaatcca ttcaatgatt tctaagaaat aatcattggc ttttttacca atcgatcgat
     1261 tcatcatcct tttgaacctc ctgtcaaatc catcccttca atgaaataac gttgtgcaaa
     1321 aaagaacagc aaaatcgtcg ggatcaagac aagtgttgct cctgccatca gataattcca
     1381 ttgtgtcgtt gcttggttct gaaaaacttg caagccaatc tgtaacgtgt atttttcttg
     1441 atcttggata tataacagag gacctaagaa atcattccaa gctgcgttga aggaattgat
     1501 cgcaatggta atcaaggcag gtttcgtcaa tggaagcatc aaatgactcc aaatatacaa
     1561 atgattcgct ccgtcgattt cagcagcttc gatcaattca ttattgatcg ataagtaaaa
     1621 ttgacgcatc agaaatatat tgaacgcatt gccgaagaac gatggaacga tcaggggcaa
     1681 ataagtacct acccagccga tcttggagaa taatacatat tgagggatca tcgttacaaa
     1741 ccccgggatc atcatcgtag aaagaaccaa agcaaacatc agctttttcc caggaaaatc
     1801 aagcttcgca aatccataag caataaaaga atttgaaagg acattaccga tcactactag
     1861 cacagtgatg aataatgtat ttcttgcata gcgccaaaaa ggaaaggcgg caatcgtatc
     1921 cagatagttt tcccatacga ttttttctgg gaaaaaggtc ggaggataac gaacgatttc
     1981 ttccattggt ttcaatgaag taaacaccat ccaaagcaat ggaatgatca aaatgattcc
     2041 tattactgtc atgaggacat aacttagtat tttttttgtt tgccgattca cactggtctt
     2101 tttgaaataa tccatcattt gccgtcacct ccagaataat agacccactt aggtgcaagc
     2161 ttcaaattga caaacgtcag aaccaacacg atcagaaaca ggatcattga catagccatc
     2221 gcataaccca tatcaaaagc tacgaacgcc ttgttccaca tatgcagatt ataaaaaagc
     2281 aaagaatttg caggtccgcc tgttccattt ttcgtcatga cataagcttc ttgaaaaatt
     2341 tggaaagaac cgatcgtaga ggtgatcaca tcgtaaaaaa taataggagt gattaatggc
     2401 aaagtaatac ttttgaactt tccccaaacg cctgctccat cgatttctgc tgattcatat
     2461 aaagatctag gaacattttg taatgtagct aagtaaagta gcattgctcc tccagaactc
     2521 catattttca tcaaaatcaa cgcaggtttc gtccattgtg gatcaaacag ccaagctggt
     2581 ccagggatat ggaaccagcc cagaaatgtg ttgatcaatc ctgcagatgg agaaagcagc
     2641 tgcatccata agatgtagac tgctactcct gataagactg ccggtaaata gaaagctgta
     2701 cggaatacac tgattccttt gattttttga ttgagtactg tggctaaaaa cacactgaaa
     2761 atcgtcgtca gcggtacatt gaagatgacg aaataaacgg tgttgtataa cgatttccaa
     2821 aataaatcat cgtgaacgaa cattcttttg aaatttgcta gaccaatgaa attcatcttg
     2881 gatgtcaaat tataatcggt gaaactacca ataactgaaa aaagcatagg ccctagcata
     2941 aataccaaga acccgataat aaaaggcgag ataaataaat aggcccatct tgcttctttt
     3001 ttctttcgga tagataaacg cttcaaattt tcttgcctcc tctgtaccta gacagtcaaa
     3061 aatggattat tgattttgct tgactaagtt ttctactgct ttttgtgcat ctgccaatcc
     3121 ttcttttggt gattttgttc ctaaaagaat ctggtcaatc tgttcattca ctaatgtcgt
     3181 gtaatccgga gcatctaaag gaactggtgt gatcaccgta tttttaaagt tttcgtccgc
     3241 catttgatag atcatttttc ctttatccgt taggttgtca ttattcacta aattctcatt
     3301 ggctgtcttg ctcgccatga tatcaaaact tttttcgccg aatttttctt gtacttctgg
     3361 agaagaaaga tatttgatga attcataaga ggcctcagga tcttttgctc cgtatggtac
     3421 ttccaagacg aatccgccac cccacgacca atggcctgaa ccttcttctt tttctgggag
     3481 ctgcatcacg ccaaaatcaa aatcatccgg tgcattttcc atcaaactag agtaataatt
     3541 gatattttgg gcacgcatgg ctactaatcc ggaaataaat ggatcagata caccagaccc
     3601 aaattcggct tctaatttat tgatcgtatt tttcccataa taatcttgcc agtcttggat
     3661 ccactccaag gcttccactt tatttttagt atcaatcttg actttttcat ctttgtcaaa
     3721 ccaactagtc ccgttatctg cgtttagcgc ccatacatca gcccctaaat tccataatgg
     3781 ataaaaaccg atgcgtgtaa attcttttcc ttctttttta tctagtttac gtgctgtttc
     3841 ttctaattca tcccaagttg tcggtagatc ttgttcactg attccagctt ctttaaataa
     3901 ctttttatta taaaaaatga cttgtgtatc tgtattgaat gggatcgcat aaggatcacc
     3961 ttcatattct actgtatccc aaagctgcgg ataaaaatcc ttagaagtgt ctttttctac
     4021 atatttggag agattagttg cttgctgtgc ttctgctctt tgggcaacgg aatttatgtc
     4081 ctgtacaata acatcgggtg catttccagc tgttattgca gataaagatt tagtccagat
     4141 gtcaccccat ggctgatact tataatttac atggatctcg ttttgtgatt gattgaaatc
     4201 ctcaatgatt tcttcgatag cttcttttcg tgcaccggat ccccaaaagg accagaaatc
     4261 aatatttttt cttccatcac ttgttttttc agaagaattt gctgcacttg aactggcatc
     4321 gcttgcatta cttgatccgc atcctcctaa taaaaacaat gctgaacttg ccagcaaccc
     4381 tgtaaacaat aattttttca tattttacct ccaaatttta atgtaaccga tgccggtttt
     4441 aatataacga tatcattttg ttctgaaaat aacaatttat aaggtatgat tctaaaaaaa
     4501 ttattaatga taccaatact ttatataatt cgcgagcgct ttctttgaat tttttaatct
     4561 ttcgtagcag aatgagaatg tattaaaaat agattaggag gaattttgat gtttagacac
     4621 cagaaagaat tgcagtttga agcaaaacca gatcgtccga atccattaat cgcgaaatat
     4681 ctgcaggagc tgatcggcgg ccaatatggc gagatgagtg tagctatgca gtacctgttc
     4741 caaggctgga gctgccgagg agaagaaaaa tataaagata tgctgatgga cattgcaaca
     4801 gaagaaatcg gccacgtgga aatgttggcg acaatggttg ctcgtttatt agagggtgcg
     4861 cctgttgaag atcaagaagc agcgatgcaa aaagatccaa caatcggtgc cattatctct
     4921 ggaatgaatc cacagcacgc gatcgtagct ggcttaggac ctcgtcctgc tgacagcgtg
     4981 ggcaatccat ggagcggtgc ctatatcatt gcgagtggca acttgttagc agactttaga
     5041 gctaatttaa atgccgaatc acaaggaaga cttcaagtag cacgtattta cgaaatgatc
     5101 gatgatcgtg gcgtaaaaga tatgctgtct gttctccttg caagagatag ctatcatcaa
     5161 aatctatggg aatgtgcagt gaaagaatta gaagaaaaag aaggttcaat cttagtccca
     5221 agtactttcc cgcgtgacaa agaacgaaaa gacattgctt atgacttcta taacttctca
     5281 gagggagacc aaagtagtga aggttcttgg gctagtggca aagcactgga tggcgaaggc
     5341 gaatatcaat ggttgaaaga acctaagatc gaaggcgctg caccaaaatt agaaccagta
     5401 acaccaaaaa tgtatgggac acctccattc aagaaataaa tatctacccg tagaaacacc
     5461 cgttttttgt tccacacgct ttgtgagcaa aactgactaa agtcattaat cataaccacc
     5521 cttcaaaagg gtggttttct ctagagctat aagctcaaat caccggccag cgcctaaaga
     5581 cgctggcttt catattttat gttcaagcca tattgccttt gccagttttg cccacctctt
     5641 aaaaggttta tgtactactc cttagccctt aaaaggatcg tcttactctc gtacactcag
     5701 cttatctaac gcactatcat gtttttcttg ttcttgaatg tactttttga tggtcgcttc
     5761 attttgtcct acttttgtca catagtatcc ttccacccaa aagtgtcgat acccaaattt
     5821 atattttaga tttgcacaat ctcttgatga tctcctgaat attggctcga tattgattat
     5881 aaattatttt tcttctatac tttggtgtga atacaatgtg gtatttacac atctactttg
     5941 tacgggctaa actatttccc tttattgctc cacccgcata gcaagtggtt ttgtatttca
     6001 atcgctcggc gcgctcaatt aggtctaaag acaataaatc taaaaacccg ctgcttcact
     6061 gacagaaaca acgggttttc agaaaaaaat gaaatgaaaa agatctctta cggcaatcta
     6121 ttttattttt ttgatttttt ataaatggaa aatgcttcaa gaagtccaag aaataaagcc
     6181 gtataaaaac aagaacctat gaaatcttta tttattagat attcaacaga aatcccgata
     6241 acactagcca acaatatatt tatcagaagt gtaaaataag gatgcttttt atagaataga
     6301 actagtgttg ttttcataga ctctcctttc ttcatatttc caattaatca caaattcacg
     6361 gcattactag aaaaattaaa ggggcgcaga agtggaaaaa tactcttcga ctttccctac
     6421 aaaccaggcc atcttgtgtt taataactat accaaatcta aggtacctct aaatctaaat
     6481 tccatttgtg gcggtgacct ccaattttta actcttctat ctcccatcca gtatacataa
     6541 ccttgatact tcgaccactt atatatcgaa cacacaaata cctatccatt gcaggagtac
     6601 tacctaactt agttgctact tgcatctaaa taacagctga aaagatagga cttactctta
     6661 gtaaagtatc tgaactatta atttttaaac tatcattttt atcctcaact acatgcacat
     6721 ctgttgcaac ttcactttct gtctcatcca gataaatgat taaggaatcg ggaattgcaa
     6781 tatcttcagc tgacgctgga atagttgagc taaattagtc atcatatctt tattataaaa
     6841 taaccaacag caaaaaatac aatgaaaaac aaacttggaa ctaatgtatc attaataaat
     6901 gttgaaaaat tcagcaactc attactgtat gattttagca gcccaaccaa catataccct
     6961 ataaaagcta ataagatggt taggaatcct ttagaatttt tactcattca tagacacttc
     7021 cttaaataac ttttattata gaaggtattg taaataataa tattgacaaa aagtgactca
     7081 aaataaaaaa aaccttacat tatactggaa tgaactgtat tgatagtgta cctaaaagct
     7141 ctactgacat aagatcgtgt aatcggtaaa gtaattttat taattatacg ggatgtaatc
     7201 aattagagaa taaactaatc ataaaaggta cttttacatt cataattgtc gctttgagat
     7261 tctactttgg ttcactatct gacccaattg cacattttcg atcattacaa gtattggtgt
     7321 cgttagaaca actaaataaa aactagcggt agatttttca gaatctatgc taatttctgt
     7381 atggatgttc cagtgatggc aaatttctct tttcattgtt tttcttgtcg catgatatgc
     7441 ctttttgtgt tattcagtta aactatagaa attctttcct agacagatct tgatcccaaa
     7501 ttttacgttt ttgagcatcg atatagttta aaatgacatt actcatatct aaagagttta
     7561 gattttttag gactaaaact gtcagttgtt ctttttcttc tttctgttaa agtattgtcg
     7621 cgacttttcg tatacgttaa gatatgtcgt gaaaaaacaa gttttgtgac acaaaaaaac
     7681 gaatgacttg aggaaatcac ctcaatcatt cgtttaaaat gctatatatt ctttagttta
     7741 agatacggaa attaatgcca tcgaccacac tagtagaatg aacgacattt acttggcctt
     7801 tttttaattt cgttatgttt agcatataaa aatattatag agtttactta caatgcttaa
     7861 aacatatact gtataatctg atcaatttta gccatgtctt gcaatggtaa gttttctatt
     7921 tttttgagtt ttcgttcctt aaaatcaaga gattttaatt gtgtaataat tacttgtcct
     7981 tttatatcta gctcttcagg taatgagtaa cgtgtcggta aattctttat agtggaagta
     8041 attggacata tgaccgaaaa cattgtttta cggttaaatt cataacgaga gacgactagt
     8101 cctggtcgtc ttttttgaat ttcttttcct acggagggat caaaatcaat ccatacaata
     8161 tcaccttttt taggaatata aattctttct tctgacacta taaaatttct ctcccttcag
     8221 gagttaaatc ttcccattcg tctttctcat aatactcggc ctcttcccca ccactaaatg
     8281 gatcttcaat ttttggaact aacgtaattg ttccatcatc agaatacaca acgatatatt
     8341 cttggttttc tgaaggtttc tttccgttat cagaaggtaa agttaccact acagaacttc
     8401 cttgtaatct agattttgta acaagcatta tcatcactcc cttaataaaa gtatactata
     8461 taacactaag taacgcaaaa ttcccctttg taaactatct agaaaattgt tttgtttaaa
     8521 gtatatctta ttggatataa atagaaataa attgtagcca gttcattttt tctttaacta
     8581 aaaaaataat ccaagaaaca ttgaacctat ggatccaaaa gctacccaaa tactatagct
     8641 aaccccccgc taccttcaat tttgcagata aaacgctttg ggagaaaatt tcaaagacaa
     8701 caggccatcc tatcatgccc gattataaac aaaaagctgg ctattccttt tgctctgaca
     8761 aatttcaatt gggaaagttc acgaaactag caggtgaaga agtttcaact gttcggatca
     8821 aagaatgccc gcttcaaata gaaacattgg ttcaacgaat ttcaactaga caagactttg
     8881 ctattgtaga acgtgagatc gtagctattt ttgtagaaga agggatttta catgatccaa
     8941 ctcatatcaa tgtggataaa tggcaacctc ttatctataa attcagagaa tatacaactg
     9001 ccaaccagtc gttaactttg aattttagat tccaagaatt caaagagtca taatgataaa
     9061 catcgttagg tataaataaa aaagactcgc agcacgagta aaaaacaaat agaagctata
     9121 cattagaaag gtcgactgtg accctataga tgcagtatat gcagcttatg agcatttctt
     9181 ctgttctctt tgtttttggg atctgtgctg aagtcttttt cgttttttgc cgctctttct
     9241 ttgaaaaatc gattatgtta taccttctcc attactaatt caggcatctg ctgagtaatg
     9301 atttttcgcg caaaagaaat atctgttacc caaggttcga tttgttcttc agtcaagaca
     9361 agcggcattc ggtcatggat tggtgcgact gtgcggttag gttcagtagt cattatgatt
     9421 gattctgcca ttttttcatc ctgatggcta cgataaaaac cgccaatata cactattttt
     9481 tccttatttg aaaacataaa tttgtctttt tcttcattcc actcattggg aaaacacaac
     9541 gattttgcaa aaatggttga tgaaaggtct ttttttcttc tactgtctct gcacgggcat
     9601 taatcatcaa ttgtcctttt ttaaatccgg taaatcccca cttcatgatt tccggagtga
     9661 ggatgttttc ttcctctttt cttaacgtca caacaagatt tgacggaaaa acttctccag
     9721 ctgctaattc tgctgcctgc ttcggttgtt tcgtctgggc ttctcgataa taattctgaa
     9781 ggagctctga ctctagatca aaaaaataac gaccacacat cctaatccct cccaatcttt
     9841 ttttctatca tacactgcaa caagaagttc gcaccaacaa aacgctttca atttttgtag
     9901 aactaaaaaa gttgtgacaa gtgacaaact ctccagtgaa tggtattctc ctgccaactc
     9961 ctcggtcaca agtcacaata ttcaacaaac atgagaagct gctttttgaa attgttcctt
    10021 gtgactcgga gtgaaacggc aggcttacga cctctgggta aacggtgttc aaaagccagt
    10081 ttctcggtat taagccaaat ttatgcaaaa atgtagaaag ctattttcac aaaatttttc
    10141 ttaatactcg aaactaagcg actttttcac aacctcttcc ttaaacggtg ttcaaaagct
    10201 tgcatctgcg gtaataagcc caaccaaacg aaaaatatga gcagctattt ttatttggct
    10261 gttcttatta cttgatgcag ggcagctttt tcacaacctc tccactaaac ggtgttcaat
    10321 cagctaacct tcggtattag ctcaaaaaac ggcaaaatat gaggaactat tttgccgttt
    10381 gtttctctaa tactcaggtc aaagcgctga tttcacaacc tctaatacac ggtgttccag
    10441 aagtagcttc ttcgaaaaat aagccgaaat ttcacaaaaa tttgagaagc aattttcgta
    10501 aattccttct tatttcttga agctgaccac ttctgtcaca acctctaatt attaaagaac
    10561 cttttgcgaa gttaacaatc tttctttgat caattgaaaa ttctgcctaa gtagtgatag
    10621 atcctgtgtg actttcaaca ataagatact tccctggatt tcagcaatca acgctttggc
    10681 ttgacggtct gcttcttcat cggacagttt ttcttcagaa agtgccagga tattttgttt
    10741 cgtatacttg acccattgat tgactaattc tagtagcaac agacggtatt cttcatccac
    10801 tgtactcaac tcaataatca aattacctac aggacagcca tgataagtga tctgtgtctc
    10861 atgatcagcg aaaatccagt ccaacatttc ttctaatgct tcttctggct gttttccagc
    10921 atttaagatg tcttcgaaca attctgtctg ccatacgtcc atatgcgcat ggatcaccgc
    10981 tagacaaact tctttttttg aagaaaagta ataataaaat tgaccttttc ctgtatcaga
    11041 agctgctaga atatctcgga ttgacgtttc atgatagcct tttttataaa taagatcttt
    11101 ggtcgcttcc aagatctttt cctttgtttt caatgatatt ctcctttttc atttctcata
    11161 gtcctatatt atacagtaaa ttcatcattt tttcttctta atcgtttaaa aaagactgtg
    11221 ccaaattatc tgacacagcc ctcactataa aactgttctc atatagctag atgttcattg
    11281 attcaagaac tgcgaggaca aaagcaattc ctatcttatg atagttgctc taatacttcc
    11341 tctgttgtca tcacttgatc tgcaataaag ccaaagttcg tcaacgctgc atccattgct
    11401 tcttggccag gagcaccaat tgcatctgtc gctaccacaa cattgaatcc actctctacc
    11461 aattcacgca tatgggaatc gctacacaag tttccgttca ttccaccaag aattactgtg
    11521 tcgattccat tttttctcaa ttggagcgcc aaatcattgc tttctggtcc aaagatttta
    11581 tgaggagagc tgatgatcgt attttcatcc aaataaggtt tcaattcttc gataaaatcg
    11641 gctcctgaac cttcttccac tgattcatat tgactttttc tagcaaacat ctgctgctca
    11701 agcatcatat gttctcctgc accgccaaat tgccatttat ggtcatgtgg gtaaaaataa
    11761 tgaggagaaa taaagagttt gtatcctttt tcttttgctt gttttaccat ttcaatcata
    11821 ttttcgatcg tattgtattt ttctaagata tcttttgtca aaccaaagcc accgcctgct
    11881 ggtttcaaga actcgacttg cgggtcagtg agaactactg ctgtattttt attattgaac
    11941 atggtggatt ctccattcgt tatttatttt aatacatcat ttagagcttc actcattgtc
    12001 ggatgagaat agatttgatc tctcaataac gtataaggaa gttttccatt catagctagc
    12061 gaaatcaagt tgatgacttc atgagaatct tccgcataga tacttgcccc tagaatcaag
    12121 tcagtttcag ggtcgactaa aactttgaat acacctttag gatcttctaa tacttgtgct
    12181 ttagggacac cgcttgtcat atacttgaat aatttgtaat tgacgccttg tgctttggct
    12241 tgtttttctg ttaaacctac attggataaa gtcggagtga taaatacact gtaaggcaca
    12301 gctttccggt tattcgttgt tcgtttattc tcgcccttca actgatccat aatgattcga
    12361 taatcatcta gagagatata agtgaactgc aatccgcctt tgacgtctcc aatcgcccaa
    12421 atgttgtctg catctgtacg aaggtattca tccacagcga ttgctccgcg atctgttagt
    12481 ttaacatctg tattttctaa tccaagtgat gctgtacttg gttttcttcc agttgcagcc
    12541 aaaacttttg acgcttggat tgttacttct tggccatctt ctgtaaaggt gacggcagct
    12601 gttgattctt ggtctgctac ttgatctaca gatacaccta gatggaattc gattcccgca
    12661 tttgtcatgt cttctaaaat catttgcgcg atatcttcat cttcacgtgg aagaaattca
    12721 gaatgggcat caagaacgac aactttagat ccgtattcat tgaacattga tgcaaattcc
    12781 aagccaatat atccagcccc gatgatcact aatttttccg gtaattgttc ctgatccatc
    12841 gcttgcgtag aatcaatcaa gtagcgactt gtattcaatc ctttgattgg taaaatgatt
    12901 ggttcagaac cagtattgat gaaaatccgt tcgccttcaa caatcacttt ttcaccatta
    12961 ttcgtcactt cgatttgatg gttagaaaga aaacgtgctg taccgtccat cactgtccca
    13021 gttgcctcat cgctgatcat atgataattt ttggcgcgaa gcatgcccgt caatttttct
    13081 ttccgagcaa cagcctctgt aaagtcgatt cctctttctc cattgaaaat caatgattta
    13141 gaaggaatac agccgatgtt gatacatgtt ccgccataca tacgtgttga cttttcaacg
    13201 actaaaaccg attcaccttt tgaagctaac gccttcgcta aagtcttacc gccctttcca
    13261 aaaccgatca caatattttg atatttttcc atcatttttc cctcgcttct tttttaccta
    13321 ccgattggta caaaaatatg ataaccgata attgtctatt ctccaaatca tctgc
//
LOCUS       DAIGOK010000079        13231 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.079, whole genome shotgun sequence.
ACCESSION   DAIGOK010000079 DAIGOK010000000
VERSION     DAIGOK010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.079"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(12..1274)
                     /locus_tag="NOA01_001834"
     CDS             complement(12..1274)
                     /locus_tag="NOA01_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI0633020.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(1267..2166)
                     /locus_tag="NOA01_001835"
     CDS             complement(1267..2166)
                     /locus_tag="NOA01_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0633021.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(2311..4089)
                     /gene="uvrC"
                     /locus_tag="NOA01_001836"
     CDS             complement(2311..4089)
                     /gene="uvrC"
                     /locus_tag="NOA01_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCI0633022.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(4271..4585)
                     /gene="trxA"
                     /locus_tag="NOA01_001837"
     CDS             complement(4271..4585)
                     /gene="trxA"
                     /locus_tag="NOA01_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HCI0633023.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(4672..7032)
                     /locus_tag="NOA01_001838"
     CDS             complement(4672..7032)
                     /locus_tag="NOA01_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCI0633024.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(7164..7694)
                     /locus_tag="NOA01_001839"
     CDS             complement(7164..7694)
                     /locus_tag="NOA01_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016630349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HCI0633025.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELW"
     gene            complement(7752..8189)
                     /gene="zapA"
                     /locus_tag="NOA01_001840"
     CDS             complement(7752..8189)
                     /gene="zapA"
                     /locus_tag="NOA01_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCI0633026.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            8391..9308
                     /locus_tag="NOA01_001841"
     CDS             8391..9308
                     /locus_tag="NOA01_001841"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HCI0633027.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(9564..10475)
                     /locus_tag="NOA01_001842"
     CDS             complement(9564..10475)
                     /locus_tag="NOA01_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCI0633028.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(10878..11456)
                     /locus_tag="NOA01_001843"
     CDS             complement(10878..11456)
                     /locus_tag="NOA01_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCI0633029.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(11476..12144)
                     /locus_tag="NOA01_001844"
     CDS             complement(11476..12144)
                     /locus_tag="NOA01_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0633030.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(12159..13229)
                     /locus_tag="NOA01_001845"
     CDS             complement(12159..13229)
                     /locus_tag="NOA01_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI0633031.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
BASE COUNT         3751 a         2843 c         2187 g         4450 t
ORIGIN      
        1 ttttctatat tttatgcttt ttttcgttca tttctcataa ttgaaatcga ttgtttcaat
       61 gaactccata atccgccttc gctatacacg agtacattcg atttgtacat gtttgcagac
      121 aagatcatca ataagaatgt tacggctaca agtaccaaca aggaaatcaa tgctccaccc
      181 ataccgactg tctcatttgc taagcgaacc ggcataatat atgaagataa gaatggaata
      241 tacgaagtga tccgaataac gatattgttt ggatctgaag ctcctagtat caatccaagc
      301 atatatccgc ctagagatag ataagtcact ggcaaaattg cttttgctgt atcttctgct
      361 ttgttgacca aagagccaca taaagctgct aaaacagaaa aaatcaaaat gccaaagatc
      421 atgaatagaa gagaatacca caagaatggt ccaatcaagt tatccaatga gataccgtct
      481 aatatatttt tgaccatttc catatctttg aattgactgt atcctatacc aaaaataata
      541 ccgtataatg ccatttgcgt taatgcaaca agtaaaacac ctgttagttt tccgtaaaaa
      601 tgcttttgtg cagtcgtact ggacaggatc acttccatga tccgcgtgcc tttttcagaa
      661 gcgatctctt gtgcaatgat ctgcgcatac gtcaaaataa taatgaacaa gacaatcgtt
      721 gctacataac ttacgacata ttggactgtc gagttatctt ctccaactgt catttttcct
      781 tttgaatcaa aattgacttt ttgtcgagtc aaacttgctt gttggctcaa gctagctact
      841 tcttctggtg aaatgcctaa ttgacttgct cgaagcatcg attgcaagcc agtcagttgt
      901 tgttgtatca gtagttgtgt actttgtcct aatgaatttt cgctataaag catcccattt
      961 atcttatcat tctcagttga gactactaaa tacgcatcga ttttttcttc cgaaagctct
     1021 ttttgtgctt tttcttcaga atcaactgct ttgaactcaa aatcatctgt tttgacttgg
     1081 ctcatttgct gactgatttg ctcattatca gccaatacgc cgattttatt gacatcacta
     1141 ttttgttgag caaagtttcc tgctacataa atgataccca gcatgataaa agggactaat
     1201 atcatgatca agaaagaaat cgacttaaca ttttttaaat agacatcttt ggtaataatc
     1261 caaaacttac gcatcgattt cacctgcttt caatttgaaa atctcttcca aagttggcgg
     1321 ttgctggtta aacataggaa tgtagccgaa ctgagtcgca cgagtaaaga tttctttccc
     1381 agcttcagga tcttctaagg taatctctaa cgtattgtct tcatgaagga tcactttttt
     1441 cacgccttcg atttcttcta cttcctgttg gcttaaacca gattccaaga ataacttcgt
     1501 acggccaaat gattcacgaa tctcatgtac ttttccattc aaaaccattt ttccgtttcg
     1561 caacatgatc aaatgatcgc agattttttc tacattatcc atattgtggc tagaaaaaat
     1621 gacacatgaa ccatgttttt tcaattccaa tattccatct tttagcaatt cagcatttac
     1681 cggatctaac ccgctgaatg gctcatccaa aatgatcaat ttaggttcat ggattaacgt
     1741 ggcgatcagc tggacttttt gctgatttcc tttagacaaa gattttactt tatccgtctt
     1801 tttccctttt acctgaaatt tttccatcca aaaatcgatt tttggttcaa tctcttttct
     1861 tgatttccct ctaagttctg caaaataaag cagttggtct tgaatcgtta ctttcggata
     1921 aagtccccgt tcctccggaa gataaccgat gacatcataa tctttctccc caagatgatg
     1981 gccattccat aacacttctc cttggtcttg tgtcaaaaaa tccaagatca aacgaaaagt
     2041 cgttgtcttc ccagccccgt tttggccaat tagccccatg atttttccat cggggatttg
     2101 gaaagagaca tgatcgacag ctgtgtagtt cccaaaagtt ttaacaagat ccttgacttc
     2161 taacatttct tcactccttc ttcaagaaat cacatatatc cttagacacc agacgtaagc
     2221 gatttattat ttaccaacaa aacgtaaacg ctctttttgt tgtacataaa atgataggac
     2281 actttacttt ctttgtaaag tgccctttcc tcattcctga tgtaatttat caaatacgtt
     2341 ttgtgcgaca tttttcggca gtccgatttc ttgtaattct tctgtcgtgg ctgctgtgat
     2401 atttttcaac gatttgaact ctttaagcag catttttttt cgttttggtc ctaacccttc
     2461 aatttcatct aatcgggaag caaaactgtt tttactgcgt agctgccgat ggaaagtgat
     2521 tgcgaatcga tggacttcat cttggattcg ctggagtaag aaaaactctg gtgaatttcg
     2581 ctcaagcggt actacagcca aatctatacc gaatagcaac tcatttgttt tatgtttatc
     2641 gttttttgcc atgccagcaa taggaatatc gattcctaat tgattttcta agacatcttt
     2701 tgccacatct acttgtcctt ttcctccatc gatcaagatc aaatcaggta aaggcaaccc
     2761 ttcgcgaatc actcgtgaat atctgcgata gatgacttct ctcattgatg cataatcatc
     2821 cggtccgttg actgttttta ttttatattt ccgatattct tttcttgccg gttttccatc
     2881 aatgaatacg accatcgcag ccacaggatt cgtccccatg atattagagt tatcaaaaga
     2941 ctcgatacgt attggggtcg ggatattcat cgcgtttcct aaacgttcaa ctgctccgat
     3001 cgtccgttcc tgttttctgg caatcagatc aaaccgttca ttcaaagcaa ctgaggcatt
     3061 cttattcgcc aattcaacta attttttctt ttctcctcgt tttggttgca gcactttcgt
     3121 agctaacaaa gcttctacac tgggcttgtc gatcgtatca ggaatcaaga cttctttagg
     3181 aatgaaatgc tcattttcct gataaaactg accgaggtaa gtcaagaaat cttcttctgc
     3241 ttcgttatag aaaggaaaca ttgaaacatc tcgttcaatt agctttcctt gtcttacgaa
     3301 aaacacttgg acacacatcc atcctttgtc aatcgcataa ccaaatacat ctcgatccag
     3361 taaatcagta tttgtcattt tttggcgagt catgattgtc tcaatggcac ggatctggtc
     3421 tcggtattct gcagcttttt caaactccat gttttcagca gctttttcca ttttctgatg
     3481 gatctctgac tcgatttttt cgtgtcctcc gtttaaaaac tttttcactt catcaacgat
     3541 tttcgtatag actttcggat ctacgtcgaa ataataagga cacagacatt gtcccaaatg
     3601 atagtaaaga caaggctcct tcgtctgact aggtttgcat tttctcagag gaaataatcg
     3661 atcgaggatc ttctttgtct cattcgccgc accgacatcg ggatatggtc caaaatacag
     3721 tgctttgtct tttagtactt tacgagtaat caccaaacgg ggatattttt cattcgttat
     3781 cttaaggaaa ggataggttt tatcatcctt taacataatg ttgtatttag gatcattttt
     3841 ctttatcaga ttgatctcta agagcaatgc ttcgatattc gattctgtaa cgatgtattc
     3901 aaagtcttcg atttcactaa caagccgttc cgttttcgta ttatggctgc cagtaaaata
     3961 tgagcggaca cgatttttta atattttggc tttgccgaca taaatgatcg ttccattttt
     4021 atctttcatc aagtaacagc cgggctgatc aggaagtaaa gctaatttgt ttttgattcg
     4081 ttcgttcatc tggtactccc cttcatattc aattaaagtt attatagcat gtctgtcaaa
     4141 aaaacaatcc agcctgacaa cgtaattata ctcaaaaaat attaatttga ataaccttaa
     4201 ctcaaccgca aaaaaaccga ggacggaact ggattcagtc ccaccctcag cttatttttg
     4261 ctgtaagttt ttataaatat tgatcgatca tttgacgtag ttgttctttt gaatgaacac
     4321 caacagcttt ttctactact tgaccatctt ttttcaacat taatgttgga atgctcatga
     4381 taccgaattg ttgaggagtt tctggatttt catccacatc cattttagca attctgaatt
     4441 cttcttcatc atattcttgt tccaactgat ccaaaatcgg tgcttgcata cgacatggac
     4501 cacaccaagt tgcccaaaaa tcaatcaata caagtccttt atctgtttct tcattaaatg
     4561 tcttatctgt aattacttgt gacatatcaa agacctccat ttttaaaatc tatgattagt
     4621 atagcatttc tatacttgga tagcgaacca tctgcttacc tttagtaagc tttacttaaa
     4681 tttaacaatt gtcgcaccat ttccgccttg gtttgccggt gcaaactcaa agcttttgac
     4741 gctccgatgg tttttaagat aatcagtaat tcccttgcga agtgcgcctg ttccttttcc
     4801 gtggacaata gtcacttgag gataaccagc tagaatggca gaatccaaat attgatctac
     4861 ctcacttagc gcttcttcgt atcgttttcc tcgaaggtcc aattgattcg gcacgtgact
     4921 actagattca gatcgtacag catggataac tcgttgtgtc ggctcttttt gaggagcaac
     4981 aggcgtcagc tcgtcttcgg acacattcat ttttaatata ccgatttcta cttgccactg
     5041 attcccgttt ttacgtaaaa gcgtccctct ttggccgtaa gtcgtaacca acacttcgtc
     5101 tcccggtttc aacgtttttt gttctttcgc cttcttcaat actttatttt tcttcaaagt
     5161 ttcttcttga tgtagatttg ctaattgggt ctttgcatcg atcaactgat gctctttcac
     5221 atttccttgc ccaatctgct gttgcatttt acgaatatca gcaatgattt tttctgcttt
     5281 ttcttccgcc tctgacacga cttcgtttgc ttttttcttt gctttttcca tttctttttc
     5341 acgttcttca aagaaatagc tgtaagcttc tttcaaatca tcgtgaagtt cttgtgcctc
     5401 agaaacaaaa tgacgcattt ccagatattc ggtctctgcc attttccggc gattctctaa
     5461 atctgtgatc atttcgttta aatcttggct ctcatcattc atcaactgct tcgcttcatc
     5521 aataacttct gtatcaagac ctaatcggct agagatttca aacgcattgc ttcgtccagg
     5581 aacgccaatc agcaaacgat acgttggact tagtgtatcc acatcgaact ccatgctggc
     5641 attaatcgta tttgcccgat tgtatccata aactttcaat tcaggataat gcgttgttgc
     5701 catcacataa gcagactttt taccaagatc atctaagatt gcgattgcta gagcagcacc
     5761 ttcttgcgga tctgttcctg caccgagctc gtcaaataat accagacttt tttcattgac
     5821 ttttgataaa atatctacag tgttcgtcat atgagaagaa aaggtcgata gactttgctc
     5881 aatcgactgt tcgtctccta tatccgcaaa tacttcctca aaaataccga tttgactttc
     5941 ttcacctgca ggaatcggta atcctgcttg ccccatcagt tgtaacagac ctaaagtctt
     6001 caacgtgatc gttttcccac ctgtgttagg tcctgtaatg acgatggctt gatagtcttt
     6061 tccgattgtg atgtcattag aaactacttt ttcctgatcg atcagtggat gtcttgcttg
     6121 tttcaagacg acatgattgt cttcactgat tcccggcacg atggctttta cttctttacc
     6181 aaatcgtgct tttgcattca tcagatccat tttaccaatc acataagcat tgtggatgat
     6241 ttcttgacga tggggaacaa gttcagcaga aagttcactt aaaatacgtt gaatctcatt
     6301 tcgttcagcg atttggtatt gacgcaaacg attattcaaa tccaccactt gttttggttc
     6361 aataaataaa gtctgtcctg aagcactttg atcgtggact acaccgccaa atacaccgcg
     6421 gtattcttgt ttgactggga taacgtagcg gtcatttcgc atggtgatga tcgcatcact
     6481 taaatacttt gcatttttcc cacggacgat cccatctaac tgttcacgaa cagcttgttc
     6541 acttcgccga atgttctgtc ttatgctctt taactcagga gaagcgtcat cagtcacgcg
     6601 tccgtcttca tcgattgctt cctttaaacg gcggctcaaa acagggatcg tgactaactg
     6661 atctgcccac atatacaaac gtgcaaattc gatttcactg tcagaaagat catcaatgaa
     6721 acgattcaat tcgcttgtcg tagacaatac tctggaaact tgtgcaagct ccactccatt
     6781 caagtctgct ccgatctcga tccgtttcat atggggacgg atattttcaa gtttaggtat
     6841 agggatcccg cctcgcagac gttgcacttt caatccatcc tcagtttctt gaagccagtt
     6901 tgtgatcgtt tgtttctcac tcactggtac caattctgcc agttcttctt ttccttgtgc
     6961 agtcactaca aattgtctga ccatctgttt gactttttca aattccaatg tttcaaggat
     7021 tcggttattc attttatcac cttatctttc actatgaaaa aagagctgtg ccggacatac
     7081 gctacgcagc cttcttaaat aaactactct gtacaaaaaa cgaaatattt tccagcacta
     7141 ttttccttaa ttcaaaatat tcgttaccaa agctcataca tcttgttggt aagaaatggc
     7201 gttctctcaa cgataaaacg agcgaaacta tgctcgccga attgattttg gaccacatct
     7261 aatggaacaa aggcaagtaa ctgcaaaatc aaaaatatag taacgtaagc catgattagt
     7321 gacaggatgc ctcctgcaat ccagtctacc tgtctcaaga tcggcacata cgtcaatcca
     7381 tggacaaaaa cccccacaaa tcgggtaagc agccagccaa ttagcaaaat aatcagaaaa
     7441 gcagttcctg cataaaaagc ttcatctagg tgaaaagcaa atacctgatc aaaaaatgct
     7501 aactttgatg ttggggtgac agctgggtat ggaatgtata attccaaatg acttgctaat
     7561 ggtttgtaaa aatgctgggc agcaataaat gacaatacat atccaatcgc gaatatgatt
     7621 tgcaaagcaa acccgcgacg tgctccagaa aaaaacgcaa ttagcaataa aaataaaatc
     7681 agcaaactca gcatgctacg ttcctttctc ttctaacttt tttaaatttt tcttattttg
     7741 ataaaaggat cttaattaga agatttttgt ttaatttgtc cttttcgttt ctcgtttaaa
     7801 atctgttgtg cttccacatg atttttaatg gtatgatctg cttgaccatt ttctttcaat
     7861 acttcccgag cttcgttctc gattgcttca atgcgtttca cacgattttc aagctctgta
     7921 aatttgatca tttttttctt taattctgct gtttcttctt ccagctccaa gattctttct
     7981 tgtttcttca attgatccga aagcgcattg acagccatca agattgctgc ttgttcatta
     8041 tccatttgtg gtgataattg tttcaattct gctaactgtt cattgatcag cttcgttacg
     8101 atatccatat gatgcttggt ttcccgtccg ataatagtat atgtttggtt ggcaatcact
     8161 gctttgtagc gtgttttttc gtgcgccata cgaatcattt cctcctattt gatgaaagtt
     8221 ttatttagtg taccatgaag ttggttcttt caccgttctc cacaaatatt tttcacatac
     8281 ttaactatta tatgataaac tttaaaaaaa tgctatgtct cgcaatctct tagaaaacga
     8341 aaagtcgtga gttgcggtat agttttatgt ataaaaacga ggagaatttt atgagcaacg
     8401 tcattatcaa agcaacaaac gaccaactta gtcaaatgaa agcatactat cgctcttatt
     8461 tgttaaataa acagattcct tatactagct ttgctgcaaa aaaagacggg acgaccatta
     8521 ccgcttatac atccggaaaa gtgatgtttc aaggtacgaa tgcagcaata gaagctgata
     8581 aatgggggga ggcacaggat aaacaagcca agccagcaaa aacagcctct tctttgccaa
     8641 aaaatatcag tcaattatcc gttttaggta gtgatgaggt cggaaatggc agctattttg
     8701 gtccgctaac agtatgtgct gcttatgtga aacgtgatca gcttgcagaa ttacgtaaac
     8761 ttggtgttag agattcaaaa gagttaaaag atagccaaat catccagctt gctgacatct
     8821 taaaaaaaac catcccctat aaactgcttg tcttatctcc agaaaaatac aatgagatcc
     8881 aaccaaaata caatgccgtt cggatgaaag tcgctttgca caatcaagcg atccatttgc
     8941 tgctaaaaaa aatcgagcca gaaaaaccag aagcgatttt gatcgatcag tttacgccgg
     9001 aagcaaattt taaaaaatat gcgcgtttag aaaaaaatca attacaagaa aaactgtact
     9061 tcgttactaa aggagaacaa tatcatttag ctgttgctgc tgcttctatc atcagtcgtg
     9121 ccagcttttt ggaagagtta cacaaagcat cagctgaagc tggaatcacc cttccatcag
     9181 gagctggaac gaaatctgac caagtagcgg caaaactatt ggagaaaggc ggcatgccta
     9241 tgcttgaaaa atatgccaaa cttcattttg ccaatacaga aaaagctttg aagctgctaa
     9301 aaaaataatg gaactgtgac aaaagtcatg tcacagtccc ttgttccgaa taaacggtgt
     9361 tctcatgcca actcctcggt cacaagtcac aatagtcatc aaacatgaga agctgttttt
     9421 tgaaattgct ccttgtgacg cggagttaaa cggcaggctc acaacctctt ccttaaacgg
     9481 tgttcaaaag cttgcatctg cgataataag cccggccaaa cgaaaaatat gaggagctat
     9541 ttttgtttgg ctgggcttat ttattactga aaataagtgg tcaatgcttg tgtcaaactt
     9601 gcagcaatct gctgctgata actgtctgtt actaattcct ttaaatcact atcattattc
     9661 atataaccca tctcgattaa aatagaaggt tgaagatttt ctcgaagaac ttgatagttc
     9721 ccagtggaga ttcctcttga atccagaggc agatcagtga aggagtcagc aattgtctgt
     9781 gctaatgttt cctcttgtcc atagtaataa tatgtcgtga ttccagttgc ttcattcgca
     9841 tattccgtag agtctgcgtg taaactgata aagacatctg cctttttttc ttcactgtag
     9901 tcgcatatat ctccaagctg tactaattca tctgtagtat gggtcaaata aaccgttgct
     9961 ccagcatcct ctagcgcctt ttttactttt atggctgtac ttaatgttat atctttttct
    10021 tctgtttgtt catcattgct taatgcccca gtatcatttc cgccatgccc aggatctaat
    10081 acaatcactg catcagatag ctcttccggt tttgtttgta tattctttgt gactttggtt
    10141 ccatcttcga gggctaaata tccagtatag ccatcatttg tcttgatctg ataagcattt
    10201 tttgtttcag aaagaaactc cgccagctct ccgccgttta tagttgcaag tggggtagtt
    10261 agcgagtcat ctgtatatag tgtacggtca ttttttccga ttttgattaa tgtctcttgt
    10321 tctgtatcat caaatgattc ctttgtcttt gtaaccgtac tgttcgtact gctgaccgtt
    10381 tgtccaacta tctttttttg accaaaaaca tctatttgaa aacagataat aatgataatg
    10441 attgcaaata caaaagctcc aattattttt ttcattcttg actctcacct cgtagatcca
    10501 gactaagggt caaaacttaa aatcagctta agtacgcaaa aaaggggctg taaaagatat
    10561 aaccgtagaa aataacatta ctgctaaaat gatttttttc atttttggca tatcctgaaa
    10621 tctccttttg actattcgtg gtgattcatc tacccaataa atagtttcct tcattctaca
    10681 aacaattaat ttcttcgtta ttaaattaaa acacttcctg gaacaccgac ataataaata
    10741 aaaatatacc ctgataatca tgagagaata acaaatttgt atttatgaaa aactacctat
    10801 ataaaaaaaa caagatataa cgatcttcgt catatcttgc ttcttcacct aactattttt
    10861 ctccctatat aacagcttta cgcttctcct aatacaccat tcaaatataa atcgatcaat
    10921 tgcgccacat ttagcggttc ttccacaggt cttttcgacc gagtgatgta aggtgctaac
    10981 actaagaaaa aatgccttgc tgcgatttct tttgaaatcc catttcttag cacagattgg
    11041 tttgctgaaa agatttcttc aaaaggttca cgataatttt tcttccatag aaagaaaaga
    11101 tgatctcttg tttcctttga aagattgctt tcaagatcgt gaagcatcag aaacaaatcg
    11161 accagatgtg tctcaatcaa aaactgcgcc atttttgtca aggttacttg aaatcccgtt
    11221 tcgcttgttt gaacgatttc tctcaaagct tttccaacat cttttaagtg gttttctaaa
    11281 actgccgtat atagtttttc tttatcaccg aaataatgat agagattggg ttgggtgatc
    11341 ccaacttttt gtgcgatctc tcttgttgaa gtgccgagat atcccttgga cataaacaag
    11401 tcagtcgctt ctttcaaaat tttttgctgt gtatctttgt tgtatcgatg gaccattttt
    11461 ccacctgctt tttggttatt ctttttcttt caatacacca tcttccatca taaatattct
    11521 atcgcatttg tcaatcagcc gtgtatcgtg agtaaccatg atcgttgctt tatttttgtc
    11581 ttttgattct tttgccaaga tatcgactac ttctcgtgcc ttttctgaat ccaaactagc
    11641 agttggttca tctgctagaa taatggacgg atcattgtac aatgcacgag cgattgcgac
    11701 cctttgccgt tctccaccag atagttcttc tggatacttg ttgatcaact tatccacgcc
    11761 aagctgctca aataattctt ttgcttcttt ttgacgatgt tcatgtttga tcttatctac
    11821 taataataac tgctttttta ctgtcagaaa aggaaccaga ttggaagctt gcaaaataaa
    11881 gccaatttct ttaaaacgta gtttaacgcg ttcactttct ttttgattac tgaattcttg
    11941 atcattgata aatacagagc cactgctagg cgtctgcaat ccgcctgcca aagtcaaaaa
    12001 agtactttta cctgaaccag aaggaccgat gattgcaaca aattcccctt tatcgatact
    12061 catggttgtt tctttcaatg ctttaactac ttggtcgcca tcttggaaac ttttttctac
    12121 ttctttaaat tctattgctt tcatgctctt cctccttctt atccaattgc cttcaacgga
    12181 tcgattttta cgattgtccg aacagaaaat agtgcgccta acacagctac taccagcatc
    12241 aacaagctaa tgacgccaaa aaacagccaa ttgctttgga atggaaccgc atcaggtaga
    12301 acaagggatg ttcccactgt tcctaataga ccaagaagaa taccaacaaa tgataaaatg
    12361 aatgtttgtg ccactacaga acgcgcaata aatcctcctg tgatcccttg tgctttcatt
    12421 acaccaaaga tattaatctt ttgcatcgtc agtacataaa tgaaaatccc gatcacaata
    12481 gccgcaataa cgatcaaaaa tccgatcata aatccaaaag tcaatacttg cgcattatat
    12541 ccaggtaatt cattgatgaa tttagaaatt ttgatctgtt ctaaatcttc aggtaaatta
    12601 ctgattttac ctctgacaac tattgcgtta atccgtgcat tttcacttgt atcttctttt
    12661 tcgaagcgga tctcttggta agcattgatg gttgtataga gaactggtga gacattgaat
    12721 ttggcatgat ccgtaaagcc agttagttta aacgtcttgt cactaccaga taacttaact
    12781 gtatctccta cggctagacc atattcttct ttcaaactaa tatcgcctac tgcctcatca
    12841 tcgttatcaa aagcttttcc ttcaacaaga ttgggcatga tgaattgatt tttatcgata
    12901 ccgaagaagc taacattgat ttttccttct tctgtactgt ctttactagt cacgactcca
    12961 ggtgtctggg caagatacgc cactttccca ccttcgactt cttctgctat cttcttagta
    13021 atcatggaca tccctagatt tgaatttgca tctttcgaaa gcacaatcga atctgcctcc
    13081 cacttgtcga ctgctgttcg attatcttga gctaagccat aagctaaccc agtcaaaaaa
    13141 aataccaggt aggcgatcaa aaacatcacc cctgcaacta acgcataacg taactttgaa
    13201 tgcataattt catttaatgc taaaaacatt t
//
LOCUS       DAIGOK010000080        12800 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.080, whole genome shotgun sequence.
ACCESSION   DAIGOK010000080 DAIGOK010000000
VERSION     DAIGOK010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.080"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="NOA01_001846"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="NOA01_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCI0633032.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="NOA01_001847"
     CDS             complement(322..891)
                     /locus_tag="NOA01_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HCI0633033.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="NOA01_001848"
     CDS             1077..1979
                     /locus_tag="NOA01_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCI0633034.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="NOA01_001849"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="NOA01_001849"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCI0633035.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="NOA01_001850"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="NOA01_001850"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCI0633036.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="NOA01_001851"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="NOA01_001851"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCI0633037.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="NOA01_001852"
     CDS             8547..8732
                     /locus_tag="NOA01_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HCI0633038.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="NOA01_001853"
     CDS             complement(8794..9990)
                     /locus_tag="NOA01_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCI0633039.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="NOA01_001854"
     CDS             complement(10160..11596)
                     /locus_tag="NOA01_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCI0633040.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="NOA01_001855"
     CDS             11889..12698
                     /locus_tag="NOA01_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCI0633041.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3819 a         2686 c         2263 g         4032 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gacaattttt gtcacaacct tttttgccga gtaaacggtg ttcaaaagcc
    12781 agtttctcgg tatgaagtca
//
LOCUS       DAIGOK010000081        12770 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.081, whole genome shotgun sequence.
ACCESSION   DAIGOK010000081 DAIGOK010000000
VERSION     DAIGOK010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.081"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="NOA01_001856"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="NOA01_001856"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCI0633042.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="NOA01_001857"
     CDS             complement(2260..2511)
                     /locus_tag="NOA01_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HCI0633043.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="NOA01_001858"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="NOA01_001858"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HCI0633044.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="NOA01_001859"
     CDS             3461..3679
                     /locus_tag="NOA01_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633045.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="NOA01_001860"
     CDS             complement(3968..4480)
                     /locus_tag="NOA01_001860"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCI0633046.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="NOA01_001861"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="NOA01_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCI0633047.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7376)
                     /locus_tag="NOA01_001862"
     CDS             complement(6933..7376)
                     /locus_tag="NOA01_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HCI0633048.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="NOA01_001863"
     CDS             complement(7377..8165)
                     /locus_tag="NOA01_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCI0633049.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /locus_tag="NOA01_001864"
     CDS             complement(8162..9103)
                     /locus_tag="NOA01_001864"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HCI0633050.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="NOA01_001865"
     CDS             complement(9280..9867)
                     /locus_tag="NOA01_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HCI0633051.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11395)
                     /gene="obgE"
                     /locus_tag="NOA01_001866"
     CDS             complement(10088..11395)
                     /gene="obgE"
                     /locus_tag="NOA01_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HCI0633052.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11698..12708
                     /gene="gap"
                     /locus_tag="NOA01_001867"
     CDS             11698..12708
                     /gene="gap"
                     /locus_tag="NOA01_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCI0633053.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
BASE COUNT         3530 a         2857 c         2270 g         4113 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgtaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgtattaat tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg
    11701 acagtaaaag taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc
    11761 aaagaagtct cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg
    11821 ttagcacaat tattgcaatt tgactctact catggtactt atcctggcac agttactgca
    11881 acagaagact caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca
    11941 agcaaaatcc cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt
    12001 tatacatctg aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt
    12061 tctgccccag ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat
    12121 gaaaacgaca aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct
    12181 tatttcttaa ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac
    12241 acttccacac aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt
    12301 tcagctgctg acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt
    12361 atccctgagc tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt
    12421 tcattaactg aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa
    12481 gcaatcaaaa aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt
    12541 tctagcgatg tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta
    12601 acaactgctg gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga
    12661 tttacttgcc aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca
    12721 atcgataaaa gaagcctttg actggatcca gtcaaaggct tctttctttt
//
LOCUS       DAIGOK010000082        12738 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.082, whole genome shotgun sequence.
ACCESSION   DAIGOK010000082 DAIGOK010000000
VERSION     DAIGOK010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.082"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(72..755)
                     /locus_tag="NOA01_001868"
     CDS             complement(72..755)
                     /locus_tag="NOA01_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCI0633054.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(773..1390)
                     /locus_tag="NOA01_001869"
     CDS             complement(773..1390)
                     /locus_tag="NOA01_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HCI0633055.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(1400..2224)
                     /locus_tag="NOA01_001870"
     CDS             complement(1400..2224)
                     /locus_tag="NOA01_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HCI0633056.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(2217..2777)
                     /locus_tag="NOA01_001871"
     CDS             complement(2217..2777)
                     /locus_tag="NOA01_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633057.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            2951..3907
                     /locus_tag="NOA01_001872"
     CDS             2951..3907
                     /locus_tag="NOA01_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCI0633058.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(4212..5168)
                     /locus_tag="NOA01_001873"
     CDS             complement(4212..5168)
                     /locus_tag="NOA01_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCI0633059.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            5272..5874
                     /locus_tag="NOA01_001874"
     CDS             5272..5874
                     /locus_tag="NOA01_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCI0633060.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(5962..7461)
                     /locus_tag="NOA01_001875"
     CDS             complement(5962..7461)
                     /locus_tag="NOA01_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCI0633061.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(7477..8001)
                     /locus_tag="NOA01_001876"
     CDS             complement(7477..8001)
                     /locus_tag="NOA01_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCI0633062.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            8299..9048
                     /locus_tag="NOA01_001877"
     CDS             8299..9048
                     /locus_tag="NOA01_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HCI0633063.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            9039..9824
                     /locus_tag="NOA01_001878"
     CDS             9039..9824
                     /locus_tag="NOA01_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HCI0633064.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            9793..10509
                     /locus_tag="NOA01_001879"
     CDS             9793..10509
                     /locus_tag="NOA01_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCI0633065.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(10611..11645)
                     /locus_tag="NOA01_001880"
     CDS             complement(10611..11645)
                     /locus_tag="NOA01_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HCI0633066.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(11649..12707)
                     /locus_tag="NOA01_001881"
     CDS             complement(11649..12707)
                     /locus_tag="NOA01_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HCI0633067.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT         3703 a         2652 c         2309 g         4074 t
ORIGIN      
        1 aaatagtact ggagtacaaa agacgaggaa gagccactat ctgctcttcc tcgtcttttg
       61 attctcacct gttattcatg attctttttc tcagtttccc gatatttttc cattttacga
      121 ctaaaaagct ctccgctact tggtggcgta tatgtgatgg catttgctcc tgcttgaatg
      181 gtctcagcaa tactttcttc cgttggacca cctgtcgcaa tgataggaag ttcaggatat
      241 tttttacgaa aatggcgtac tgcttctgca gtttctttac ctgcactgat attgataatc
      301 gaaataccag ctgctaactt ttcatctatt tctgtatatt tcgacgttac cgtactgata
      361 actggaatat caactactcg acaaacttct tttactgtct ctaaaggcgt aggaccgttc
      421 aaaacgaccc ctaacgaacc ttgcgcttct gcgaacatgc tcatgtagga agaacgagca
      481 ccttgtgtca aaccaccacc tacaccggag aaaactggaa tgtcagctgc ctcaataatg
      541 cttttagtaa tcgctggatg aggtgtaaaa ggatacacag caattactgc attcgcattt
      601 gtattacgta tgatcgcaat atccgtagta aagatgatcg aacggatcaa ttttccaaat
      661 atcttgatcc cgcttgcttc taaaatgatt tccggtactc ttacgatatc ttttctcaat
      721 tccgtcatga tttctggcgt aaattgttct gccatcagcc acttctcctt tgctatctcc
      781 ggtcatactg attcaattga tacgtctcga tgacctggat gattttttgc gcataaccgg
      841 ggtctgtcgc ataacctgca tcttgcaatg cttgtgcggc ttcctgatag ttggtggccg
      901 taatcacttt ttcatacagc gcaggattcc aatctactcc ttggacgaac agttgtgtat
      961 ggtcatccat ggactgttcc catgaatcat acactttgaa gtctccttgg atagttatcc
     1021 attcttcgtt aacaaattcc tttgtttcca ggcttacttt cttctgatcc ccataagcct
     1081 tgatcccgaa aagattattg tatttgctag ccaaggtact ttttccccaa tttgattcta
     1141 atatagcttg tcctaaaatg atactaggaa gcactccata accatcttgt agttcttggg
     1201 catgcggaac aagccgttca ataaattgct gatgggattg cgcttcattt tcttgctgat
     1261 tatcagtaaa aggagaagac agattcttca aggaaaaaac aaaggctaag agtatcactg
     1321 ctacaccggc aaccactgct ggccaacgga tattcttgcg cttttgcttt ttccactttt
     1381 ttttcgccat gatcaattcc tacttttcat ttaattcatt cagatattct tctagcgtca
     1441 attggtgttt ggtgatatac tcagcgtttt ctttaccgac ataacgtaag tgccaaggtt
     1501 catatgtgat cttagttata tcttcgtgtc cttttggata acgaacgata aatccatact
     1561 gcgcagcatt atctgcgatc catttagctc caggctgatc tccgtaactt gcatccaata
     1621 cagtagaagg ataactgttg taccagtttt gatcgacgac atcgactgcc agacctgtgt
     1681 ggtgttcgct gtatcctggc tccgtaatcg tttctttagt aatctttgtc gcctcctctt
     1741 ctgatacacc ttgacgactc atcacatctt ggacattttg ttcgaaaact tgttgttggg
     1801 atgatacaga ccggtaagct gagaccatca ctaatggaaa tccagctgct tcagcagcct
     1861 tggcaaatgc ttcatattct ggagcaatgc gtttgtcgat caagtatccg ttacttaatg
     1921 atgccaattg agtagactca tcaatttctt tttctaattt gtgatctgga ccaaccaaaa
     1981 ctagcttcca atcgtctgtt ttaacatctg gcaattgttt ggacgtacga tcttttgttt
     2041 ctttgtcctt taccttgctg ctggttgttt gactgttctt ttctttacta gcagttgttt
     2101 tggtgcttgt tcgttcgact gcttgattgc cggcttgaac atgcgaatca tcatcgctaa
     2161 aaataacata gcccattgct gcgatcataa tcacaactgc tccaaatcct aaaaatttat
     2221 tcacttgaaa aatcctttcg ttgttcttca cgttccccat tcgcctcatg aacgtaagtt
     2281 tcttccagat tttcattcac ccaatcgaga agttcgattt tttctccttc gatctgtttt
     2341 tgaacatctg ctggcagacg aaaattcaaa atatcatcat agccccattc atcatattga
     2401 atacttactt tcaagtcaag gtatctacca gcctgctgat tatttttcaa agggatcagc
     2461 tcgacttttg ctgctttgct gctaagccat ttttgcgcga tgcgtgtttt gatttgcttg
     2521 tactctgcaa cggcaacgtt tcgtttttcg ggaaaaataa tcgtttgacg caaaatcttt
     2581 tgcgttagca tccattcata atcagtgaaa agatttttta ctttctgcat cgcttcagat
     2641 ggcagatttt tctcgccatc tttccatttt tgccattgtt ctggactgat cagcaagtat
     2701 tcatctaaaa aatttttttc agtttcaaac tgttctaata atacactcat aataagatca
     2761 atatatactt gatgcacgtt tatcccctcc ttttaactat tctacttaag ttttgatcaa
     2821 aaagcacctc ttaaatcgct gctttcattt tttttaatgt ttcaagtcct ctcctcttta
     2881 ttcttcgtga tttttgttat aataatagac agacaagaaa ctcaatccaa ctatgaagga
     2941 gcgttttcag atgaaaaaat atcaatgggg aatcgttggt cttggtacca tcgcacatga
     3001 gtttgccgaa agcttcaatc aagagacgag cgaactaaca gcagttgctt ccagaacttc
     3061 agaaaaagcg gaaaacttcg ctcaccgtta caatattcca aaagcttacg gaagctatca
     3121 agaaatgtta gacgatgctg aaatcgacat cgtgtacatt gctgtaccaa atagacagca
     3181 tatcgaccat atcttggcag cattggaagc aggcaaacac gttctatgtg aaaaagccat
     3241 cacgatgaat aaaaaagaac tggctgacgc tatgcgctta gcagaagaaa agaatttgat
     3301 cttagcagaa gcgatgacaa tcttcaacat gccactttat cagcagttac gctcaataat
     3361 ggatacagga aaactggggg ctttaaaaat gatccaagca cctttcggca gttacaaaga
     3421 accagaccct aaaaaccggt tcttcaatcc agaattagct ggaggagctc tgttggatat
     3481 cggtacgtat gctgtatctt tcgctcgttt ctttttgagc tctcaacctg aagtagttgc
     3541 ctctacaatg gttccttttg aaacaggcgt ggacgaacaa tccgtcacta tcttgcgtaa
     3601 caaagaaaat gaacttgctg ctgtcagctt gacttttcag gcaaaaatgc caaaagtcgg
     3661 tatcgttgcc tttgagaacg gttatgtgac gattgctgat tacccgcgtg ctgatcgtgc
     3721 ggagatctta tttacagatg gtacaaagga attcatcgaa agcgggaata catctgaagc
     3781 aatgaattac gaaataaaga atatggtaca catgattgaa ggtgacttgc ccaatcgctc
     3841 tctcttcttg acaaaagatg tcatcgaaat cttggatcaa atgcaagttc tatggaaaaa
     3901 tatgtaattt taaaaggttg tgaaaaagct gttctgcatc aagtaataag aacagccaaa
     3961 caaaaatagc tcctcctatt tttcgtttgg ctggacttat taccgcagag gaaagctttt
     4021 gaacaccgtt taagaaagag gttgtgagcc taccgttcag ctccgagtca caaggagcaa
     4081 tttcaaaaaa cagcttctca tgtttgttga atattgtgac ttgtgaccga ggagttgaca
     4141 ggagaacacc gtttaaagaa gaggttgtga aatcagcttt ttgacctgaa tattagctga
     4201 attcacaacc tctatttttg gattttttgt tgggagaatt ctgccttgat ttcaataaaa
     4261 cttttaaaag actgcaaaaa ctggaacaat tctgctcgat cctcgaattc agggcgacta
     4321 gtcggcagag gcatttgccg atacaataca taaacttctt caatacgtgt taaaatttgt
     4381 ctgccatcat ttgactctga aaatgtttct gcagtataaa tcaataaacc atagatatgt
     4441 tttccataat aagctggttg ctggattctc tctagatttt ccagcatgtc tttaatcaca
     4501 ttggcctgtg ctcttctcat agagaagtaa gtttcgtagt atagcggctg tctgagccag
     4561 tagttttctt gatgttctct ggcatcttct tggctttcgc gaatgaatgt cagtaaatgt
     4621 tcgcatttga tctgcacatc ttctttattt tctgataaaa gaaattctgc catttgtctc
     4681 aaaatatttc gaaattcttt ttcgatctgt atttggtttt ccttcagctg cttcgtacga
     4741 tctggcatgt aaacatttgc aagcaaagca aatactaatc caatcgccat taatcccgct
     4801 tcattgccga tcagctgcca agacatgttt tcctccacta aataatgagt gaccaaaacc
     4861 gaatttacta ctatcccttc ggttaatttg aagcgagagg caagcgggat gaacaggaat
     4921 aaaaaaagac tgaagccaaa aatagtatac ccaacagcaa aaaaactgaa cagtgctgtt
     4981 gccgtagcta atataaaggc tgcgattcga ttcatgcccg tacgcaatgt agagcgcttg
     5041 gtatttccta cacttaataa tgcaatgatc cctgcagctg gccaatacaa cagcgataag
     5101 ctacccgctg tgaccatcga aaagaagaca ctgatcactg ttttgactgt tcgcattcct
     5161 atacgcattt acttccccct tttttatttt aattatactt gaataatgaa aaactagcta
     5221 tcagacaaaa gtttaggtat gatagtaaaa agaccaaaaa ggagaatctc tatgcaattt
     5281 ggccgttttc gactaggtat gcgtacgatc aaaagtgcct tagctgtttt tttatgtatc
     5341 ttgttttttc atttgactca acgaggaatc ccgatgattg ctgcattatc tgctgttttt
     5401 tctttgcgtc aggacttgac tacaaccgtt tcttttggaa agtctaggat catcggcaat
     5461 acattaggtg gtacattagc tatcatttat ttttttgtca aaagctattt tcacaatgat
     5521 tttttggtag aattgatctt gcttcctatt ctagttatta ttgtgatcgt cgtgtcagat
     5581 ggtattaaca ataattccgg tatcatttcc gcaatagcta cgttgttgct gattgcgttg
     5641 agcatccctc aaggcgaatc cgctttgtat gctatccaac gtgtactaga tacttttata
     5701 gggacattca ttgcaatcgg tatcaatttc tttcttcgtc ctcctgaaac agaaaagaag
     5761 gaagaactat cagaagattt ggtcgaactc caaaagaaag aacgattgct aagaacaaat
     5821 ttacaagaga tagaagctaa gatccaaaaa caaaagaaaa atagcgagga ctaattttct
     5881 tttgcaataa aataaaaaaa tggagagaag aactttaaaa agtcttcctc catttttgat
     5941 tgaacggtga agtgtactac tttattcttc cttcatcgag ttttgttctg cctcttttgg
     6001 cgcttcatac atatttactt ggctagtagt cccacgtgtc gacaaattct ctacgatttc
     6061 cttcaagtca agtcctgttg tcgcttttag tgtttcttga gtagaagaaa gcaaatttgt
     6121 ggcataattt gtcactcggt tcgctccact ggtttctcct gaactgcttc ctgtatcgac
     6181 aacagagatt ttttcgatat ttcctagcgg ctgtgctgct tcgcgcatca attgcggtag
     6241 catatcgata accatactca agacagctgc ttcgccatat tccttgaagg cattggcgat
     6301 cttttgtttt gcttcggctt ctgcttttcc ttttgctagt gctgcttcgg cttctgcttg
     6361 tccagctaaa cgtgtcttat tggcttccgc ttctgccagt gcttccacac ggaatcgttc
     6421 tgcttcggct tcagtcactt ctttgacttt ttgagcttgt gcttcttgtt ctttagcgta
     6481 ccgatcggca tctgcttttt tcttcacttc cgaatcatat tgcttttcac gacgtgtgat
     6541 ttctttttct tccagttcga tttgtttttg gcgttctacg attttaatct gcatttcctg
     6601 ttcgattact tgttgttgtg ctcgggcact ttctaaattg taggcttggt ctgcttttgc
     6661 tttagcaaca tcttgttctt gtttgtaggt tgctaatttc agttcttttt cttttaatga
     6721 ttctgcgatc tctgtttgtc gctgtaattc agctgcttgt gattcttttt ctgcttgagc
     6781 ttttttgata cgcgtttctt ttagagcttc cgcttctgct atatctgcat ctcttttgac
     6841 ttgggcgatt cttggtttcc ctaaagagtc aagataaccg tttttgtctc tgacctcttt
     6901 gatagtgaaa gaaacgatga tcaaccccat ttttgcaagg tctacagaag cgacttcttg
     6961 tacgctttga ctgaacttat cgcgattttg atagatttct tctacagtca tagaaccaag
     7021 aatcgagcgc aagtgtcctt ctaatacttc tcttgcttca ttttccagtt cttctcttgt
     7081 cttgcctaaa aactgttctg ctgctgtagc aatctcttct acagatgaac cgattttgat
     7141 gatcgaagta ccatcacaca tcaccggtac gccttgttcc gtatacactt caggggtgga
     7201 gacgtccaat ttactcgata atagactgat ccgattcgaa cgttgaaaaa ctggcaagac
     7261 gaatgcgcca cctccacgca caattttgat tttgttattg ctttcatcaa tgtgcacatt
     7321 tttgcttcct aaataacttc cactgatgat caacgcttca tccggttttg ctgtctggta
     7381 cttcgctaca aagacgatca gcaacatcag taaaatcaaa gctgcgattg cgatcgcgat
     7441 aaaaacaact ggtatttcca tttaacacac gctcctttaa gcttttatca tgctttcata
     7501 aggcatgaca taacagattc tttccttgat ctcaatgatc aatactttcg ttccccgctc
     7561 agctgtctgt tcttgggggc tgtaaaaaca agctggacga ctaatggaac cggtaacact
     7621 tttcaactga atttctccca tacctcgtac agggatcggt gtgatcacaa cagctacttg
     7681 tccttccaga gttttttcag aagacgacat cgttgattcg gcatgtttca tcggcatcaa
     7741 tatatagaaa ttaagcaaaa ataccaacac tatcgatatg cctccactag caataaataa
     7801 aaataaagat gattgatctg ttaaacgctc acctagaaaa ccaaataagc ttgtgaaagt
     7861 taaccaaggg ataataagca tcggatcaag cgggccatca aaatcgaata catctccaaa
     7921 aataatcaat aggactgcca atcctgcaca aatcagcaat gtataccaat aaatcgtctc
     7981 aagtgcgatt ccccctatca tatacacgcc tccttccatt tctacgtcta tcataccaat
     8041 gtttgaaaat tctgtatagc ttcttttacc gactcagtgg tgttttttat aggaaggttg
     8101 tgtttatcgt tgacaatttg ttaaattttt gttttcatgt taaaattttc acaataaatt
     8161 ccttcgaggt agcctatgtt tccttttttt aagaaaaaac cattaaagct ccagaacacg
     8221 atcatccttg tcgtttgttt ctccacattc gtttctttga ttgtttctgc catattgatc
     8281 aggaatttta ttctttcgat gaaccgatct agtgtgtcag ataaaatctc tagcattgct
     8341 aaaatcgttg ccaatgataa actcatccaa aaaaagctgc agactggtga cccgacacat
     8401 gcgattcaag gaataacaaa tgaaatcatg attgcatcga atttagattt tatcgttgtc
     8461 atggaccctg aaggcattcg atattctcac gcagacccaa gttacatcgg tcttcctttc
     8521 tcaagtcatg aagatgtgaa aaaagtgatt ggaggacaag aatttttctc tgaaaaagaa
     8581 ggaatgttag ggttcggctt ccgctatttc gtccctattt attatgatca agagattatt
     8641 ggtgcagttt gtgttggctt aacaaacaac acgattgaca atcaagtcaa agatggacaa
     8701 aaaatgattg ttttttcatt gattattgga cttagcgtag gcatcatagg cgccgttttt
     8761 cttgcccaaa aaatgaaaaa agtactgctt ggacttgaac cacaagaaat agcagaacag
     8821 ttaagtgaaa aaacgattat cgaaaatgaa gtagatgaag gaatcttagc catctcttct
     8881 gataaaacta tcctcttgat caatacacct gcaaaaaaca ttttctacca aacagagcag
     8941 ctattggatt tcgaagtagg aaaaaaatca gtgataatct atatgacgtt ttgtttaaag
     9001 agacgattga gcatcaaacg accgttcgga ctcaatccat gtatttaaat gggatagaaa
     9061 ctatttctag cgttacacct ctttttagca atgaccgttt tttaggagca gtcgcaactt
     9121 tacgaaatcg atcagaagta atgaaattaa tggaggagct tggggggacg aaacaatata
     9181 taaactccat gagagctcaa acccatgaat tcatgaataa acttcatgtc atttctggtt
     9241 tgattgattt gaagaagtat gaagaagtca cccattttat ccagcagcta aatcaaaatt
     9301 ataaaaaaga gattggccaa gtctcaagtc tgatcaaagt acctgcaatt gccggttttt
     9361 tattaggcaa aataaacgaa gccaaagaac aatctgttga aatgctgtta gaaagcgatt
     9421 cttttatccc taaaatggag atggatgact ccatccattc acttttgcaa gtgttaggga
     9481 acttacttga taatgcaaaa gaagccgtgc tacaaaaaca tgaaagaaac ggacaggtcc
     9541 ggattcattt aaactatgac acagaagctg aagtattcat cgtgaccgtc tgcgatgacg
     9601 gtgtcgggat atcagaagaa atctcagaaa aaatatttac gctaggtttt tcgacgaagg
     9661 gaatcaatcg tgggtttgga ttgaatctca tccattcaat cgtcaaacgc cacggtggat
     9721 ttttagaatt ttccaaacaa acaaatggcg gtacaatcgt ctatctagaa ttaccaaaaa
     9781 aggaagatca aaatgatgac aaatgtcctg ataatcgaag atgatccaat ggttgcagaa
     9841 ttgaatcgta cttttgtgga aagcatccct aatatgaacg taatcgaaag tttcagaaat
     9901 gtagaagaag gaaaaaaata tttcagacaa aaaaacgcag atctgctgct gcttgatgta
     9961 tacatgcctg gcgaaagtgg tatccatttt ttatcttggc ttagacagca aggaaatgat
    10021 atcccagtga ttttgatcac tgctgcagat gatatcgaaa ccataaagaa tgtgatccgc
    10081 tacggtgcct tagattattt gatcaaaccg ttcactttgc aacgttttca gctggcgatc
    10141 gaaaagtatc ttgctttctc tcagacaatc caaacgactg aaaaagccga tcagaaattg
    10201 atcgatcaag tattcaacca caccactccc attcacgaaa taccatcatt catcgatttg
    10261 cctaaaggtc tgtcaaaact gactttaaaa aaaataagtg catttattct aaaagaagat
    10321 actatttttt caacaaaaga actggctcaa aaaagctcac tgtcacgtat atccgtcaag
    10381 aaatatttga attttttagt agaacaagat atattgaacg aaagaatcaa atatttggaa
    10441 atcggccggc cgctaacctg ttatgaattg acaaagaatg ggcggaaaaa gctcacagat
    10501 tactgttgag tcatcgaatt atcccataca aaatagtcca agtatacaga agtaacaact
    10561 gtttacttgg actattttta aacattttat tgacgaatat ccatgttttc ttatcttaat
    10621 tgacgggctt taggaagaga tacctcattt ttcgtttttc cagtttctat tatttcacga
    10681 aagttctcga aacttgtcga aatcatattt gataatgcct catctgtatt tgaacctaca
    10741 tgcggggtta caagaactct agggtacatt tctactaatt gctggatagc agggtctgga
    10801 atcgtctccc atggcttgaa ggatcggaaa aaaacctctg tttcatttgc aaaaacatcg
    10861 gttgcaaaac cagctaaatg attgcttttc aaagctctta aaatagcttc attatcttga
    10921 agctctccac gggctgtatt aatcaagatg ctaccttttt tcatttgagc taaaaatcct
    10981 tcattgatca tttgatcgtt ttcacctggg atatgaggta catgaatact gacaatatcg
    11041 cttttggcta ataactcttc aagcgagtgg aacgtcaata cttttttagc agcttctgtc
    11101 tgatgaatat cgtagccgat cactttggct cctagtccct taaacaaaac agcttccgtc
    11161 aaccctatcc tgcctgttcc tatgatccct actgtgcaat ttctgatttc acgactaaac
    11221 atttgctcat ccactcgaaa atctttgaaa gatgttcgga tcgtcgtata cgcagtattt
    11281 ctcaaaagca tcatggcaaa agtcaaagat aactctgcta tcgcatttgg cgaatacgat
    11341 ggtacacggg ctactgtcat accaaaatca gctgccgctt gtagatcgat atgattgact
    11401 cccacagtcc gtgtaaaaac ataacgaatc ccatattcct gcatttttgc aagattggtc
    11461 tcatctgcta tacaatttcc cctcaacaat acagcatcat ggtcctttgc tgacacgata
    11521 ttgtcatgtg tcaacagttc ttcataaaga ctgagatcat aatggtaaat attcaatttt
    11581 tcaaaaaaat ctatctcgtt cggacgaaca ccgtaacatg cgattctcat actttcttcc
    11641 tccatttctt atgaaaataa acctatgcca tgaatcattt gtaatacaac gatccataca
    11701 ggtgtcagta ccacagcaaa aatagttgac agtaaggatg cattggaggc taaaagtgcc
    11761 tccctatcaa aactgatagc ataggctgct gccactgttg cagctggtgt agccatcatg
    11821 ataacgattg ttgccagtgc ttcaaaattg actggtagaa tattcgttgc cgtcaaaact
    11881 gctagaatca cgatattcaa tgcaggtaca aataccactt tgactagact gtaataccat
    11941 gaagtttttt cagatgcagc ttttttgaag cttacttcac ctaatgttgc gccgattgct
    12001 agccacgcca atggtgaact taaaccagct aaataatcca tcggctgaaa tagccacggt
    12061 gcagtctggt caattcgcaa aaacgctact tggcttatct ctccttctgc acctgtaact
    12121 gacacttgtg gcagataccc ttggaacagc caaatgaaaa ggccggcaaa agtagcgata
    12181 atgattggat tgacgaacat cgctttgata tttttagctt ccatcttcaa gccactcatt
    12241 tttatgtatc cataagaata caaaaagatt cggtagccaa tattgaaaat tgaggaatac
    12301 atcacgccga caggaccata aatcgcactg acgataggcg ttccgaaaaa ggtcgtagaa
    12361 ccaaagatcg tcaggacacg caacgttgtt tgcttatccc ctttatagct taagaaaaaa
    12421 ggttttgaaa ctagaataag aataatataa ataactaatc cccaaatcaa gacattgatt
    12481 ccttgcatca gtaattctgt attgatatct tgcatgaaag ctcgaaatgc cagtgctggc
    12541 aaagccactg ttaagacgac tttcgataga atttttccaa cagattcaga aaaaatcccc
    12601 ttttttctac aaacaaaccc taataatata ataaaaactg ttgaagtgat tgcactgaca
    12661 atcgacatat ctgtcaaagt cgatcgaaaa atgtcaaata agttcatctt tctcctccta
    12721 attatgttca tttattca
//
LOCUS       DAIGOK010000083        12547 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.083, whole genome shotgun sequence.
ACCESSION   DAIGOK010000083 DAIGOK010000000
VERSION     DAIGOK010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.083"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            22..591
                     /locus_tag="NOA01_001882"
     CDS             22..591
                     /locus_tag="NOA01_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HCI0633068.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            578..1438
                     /locus_tag="NOA01_001883"
     CDS             578..1438
                     /locus_tag="NOA01_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HCI0633069.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            1628..2332
                     /locus_tag="NOA01_001884"
     CDS             1628..2332
                     /locus_tag="NOA01_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCI0633070.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2337..4172
                     /gene="walK"
                     /locus_tag="NOA01_001885"
     CDS             2337..4172
                     /gene="walK"
                     /locus_tag="NOA01_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HCI0633071.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4169..5485
                     /locus_tag="NOA01_001886"
     CDS             4169..5485
                     /locus_tag="NOA01_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633072.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5486..6355
                     /locus_tag="NOA01_001887"
     CDS             5486..6355
                     /locus_tag="NOA01_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HCI0633073.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6410..7219
                     /locus_tag="NOA01_001888"
     CDS             6410..7219
                     /locus_tag="NOA01_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCI0633074.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7322..7951)
                     /locus_tag="NOA01_001889"
     CDS             complement(7322..7951)
                     /locus_tag="NOA01_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCI0633075.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8121..9413
                     /locus_tag="NOA01_001890"
     CDS             8121..9413
                     /locus_tag="NOA01_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCI0633076.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9489..10151)
                     /locus_tag="NOA01_001891"
     CDS             complement(9489..10151)
                     /locus_tag="NOA01_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCI0633077.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10376..10660
                     /gene="groES"
                     /locus_tag="NOA01_001892"
     CDS             10376..10660
                     /gene="groES"
                     /locus_tag="NOA01_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCI0633078.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            10711..12336
                     /gene="groL"
                     /locus_tag="NOA01_001893"
     CDS             10711..12336
                     /gene="groL"
                     /locus_tag="NOA01_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCI0633079.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4177 a         2143 c         2741 g         3486 t
ORIGIN      
        1 atgaaaaaag gagcggttat gatggaaaaa atgcgtcagc aattaatgga gtgggggaag
       61 gaactagaaa cattccgtct gcctcactgg gaagaactcc ctgacttaga actttatatg
      121 gaccaagtca gaacgttagt tgatcgttat ctttcacctg tgatacaagg agaaaagcat
      181 ccattgctta cctcttctat ggtaaacaat tacgttaaac taggtttgat accagcacca
      241 gttaaaaaaa gatataacaa agaacacgta gcatttttac ttgcaatcac tacgttgaaa
      301 caagtattga ctattcctga aatcaaagaa gggatattgt tccaaggaaa aacagtagga
      361 attcgtgaag cctataatct attttgcgat gaacaagaag cagctgtgtg gatggtcagc
      421 caattagcac aaggtaaatc tcatccgcaa aaattcgacc aggcaacccc tgtagaatat
      481 attgctgtaa gagcggcaac tttatcgttt gcaatgaaac ttcttgcgga aaaaacgatt
      541 gttctagaaa ccgaatattt aaaagaggag aaaacaaatg aaaaacaata aaatcgcaat
      601 tttagtagat tcaggtacgg atgtgccaga agaattgata aaaaaatacg agatgtatat
      661 gatcccattg aaaattattt ataaagatca ggtatataca gataaaattg acattacctc
      721 tgaagagatc tatcaacgtt tgccagagga aatccctggt acatctttgc cggatggtga
      781 gtcgatcaca gaaatctttg agcaaatcaa aaaagacggt tatgaaaagg tacttgcggt
      841 aacaatttcc agtggattga gcgggacata taatgtggtt cgtttaattg cacaacagca
      901 agaagaattg gaagcatatg tactagatac gaaaaatatt ggtattggtg caggtttttc
      961 tgcgatccaa gcagcaaaat ggctggaaga aggagtagaa tgggatcaac tgatcagtaa
     1021 tctgaatgag ttagtcgaaa gaacaaaagt tttctttaat gttgcgacat tggaatatct
     1081 tcaaaaaggt ggacggatcg gtttagttgc ttctattgta ggaaccgctt taaagcttaa
     1141 cccaatcatc tcatgtaatg aagaagggat ttattacaca gtaggaaaag ctaggggacg
     1201 taaaaaaagt ttggataaaa cggttgctta cgtcaaagaa aaaatcggta atgcaaaggt
     1261 cttcaactta gctgttgccc atggggatgc gaaagaagaa gcaattgaaa tggaagcgag
     1321 acttaaaaga gagttcccac aagcaaacca aatctacttt ggccagattt ctccggcatt
     1381 agtagtccac actggtccgg gattattggg tgtcggtgta caagttttag aaggataaat
     1441 caaaaaaagg aaacactgag gtgaaaatca gtgttttcct tttttattga agttagaaca
     1501 aaaaataata aagtattttg cactttcatt ttaattttct cttttgtttt ataggcaaag
     1561 atgaaacaaa agcagaaata cggtataatg agaaggtatg acaataatat ataagaggtg
     1621 gctctttatg aaaaaagttt tagttgtcga tgatgagaag ccgatttcgg atatcgtcaa
     1681 attcaattta gccaaagaag gctatgatgt ttatactgca tatgatgggg aagaagcctt
     1741 ggaaaaagtt gctgaggtag aacctgattt gattttactt gatttgatgc tacctaaaat
     1801 ggacggctta gaagttgctc gtgaggtacg caaaacttat gatatgccga tcattatggt
     1861 aacagccaaa gattctgaaa ttgataaagt cctaggctta gagttaggcg ccgatgatta
     1921 cgtgaccaag ccattttcca accgagaatt agttgcacgt gtcaaagcaa atcttcgtcg
     1981 cggtgccacg gctgcgaagg aaccagaaga ggcagcacct gcggaattga cgattggtga
     2041 tttgacgatc catccagaag cttacatggt gacgaaacga ggagaaacaa tcgagttaac
     2101 acatcgtgaa ttcgaactac tcttttactt ggcgaagcac cttggacaag tgatgacaag
     2161 agaacattta ttacaaacgg tttggggtta tgattatttt ggagatgtac gaacagtgga
     2221 tgtaactgta cgtcgtttgc gtgaaaagat cgaagataat ccaagccatc caaattattt
     2281 agtgacacgt cgtggtgtcg gttactattt gcgtaaccca gaacaggagt aattggatga
     2341 aaagaaaagt ccgctttttt cgatcagtca attttaagat cgctattacc tttattctga
     2401 ttttattgat ctcgatcgaa attattggtg cctactttat acgtggttta gaacgttcca
     2461 cgatcaatac atttattaaa gatatgaatc aaacggtgga atcattagca acaacaatca
     2521 gcccggaatt gaatcgaaaa gataatgcag atgatgaaga ggtaaatgcg aatatcaaaa
     2581 gatttattga aaacagtgcg actagcgata tcatcgaaat acgggtcgtt gacgaaaaag
     2641 ggatcatccg aggaacaacc gatgtcaatg aacaaagcgc tgtgggaaag aaaaatgatt
     2701 atgttgatat caatgatttc acgactaaac gttatgttgc tttagataac gacgataaac
     2761 gggtgaatat caatgttcaa ccgatcctgt ccccgaccgg cgatgccgtt atcggggctc
     2821 tttacgtcaa aagtaatatc gaacaaaaat attcggaaat caataacaca gcagtcattt
     2881 tctttactgc ctcactaatt gcggcattta tttcaatgat cgtatctgtt ttagttgctc
     2941 gttcgattac acagccgatc ggtgaaatga gagaacaagc cattcgaatc gctagaggag
     3001 actacagtcg gaaagtcaaa gtgcatggac aagatgaact tggacagctg gctgatacct
     3061 tcaatcagtt agctgaacgt attgaagaag ctcaagatac aatggaagct gaacgcaatc
     3121 gtttggatag cgtgctatcc catatgacag acggcgtcat tgcgacagat cgcaggggta
     3181 aagtcattac gatcaatgat atggctgttt ccttgctaga tatcaaaaac gaggaagcaa
     3241 tcggacaatc gattttgaca cttttagata tcgaagaaga atatacgttg cggaaattat
     3301 tagaagatcc aaacgaaatg gtacttgacc gttcaaccag tacattggaa agtgatcaga
     3361 tgatcttgcg agtagacttt tctatgatcc gtcgcgaatc aggttttatc agcggattag
     3421 tagctgtctt gcatgatgtg acagaacaag aaaaaacaga acgggaacgt cgtgaattcg
     3481 tctctaatgt ttcccatgaa cttcgtacac cgttgacaag tatgcgtagt tatatcgaag
     3541 cattgagcga aggtgcgtgg aaagacgagg aagtagcacc aaattttttg aaggtaacgc
     3601 tagatgaaac agaccgaatg attcgaatga tcaatgacct cttaaatcta tctcgcatgg
     3661 atacaggtaa tacccagctt caattagaat atgtcaattt caacgagtta gtgaattttg
     3721 ttcttgaccg gtttgatatg atggtgggaa atcaggaaaa aaattataag atccgtcgtg
     3781 aatttacgca aagagatctt tgggtcgaag tagatacaga taaaatcatc caagtagtcg
     3841 ataatatcat gaataacgcg atcaagtatt caccagatgg gggaacgatt acttgtcgct
     3901 tattagaaac gcataataac gtggttttaa gtatcaccga tcaaggattg gggattccta
     3961 aaaaagatct gaaccgagtg ttcgagcggt tctatcgagt agataaagca cgtgcaagag
     4021 cacaaggtgg gacaggactt ggattagcta tttctagaga agtgattaag gcgcatcgag
     4081 gcgctatttg ggcagagagc aaggaaggaa aagggtcgac tttttatatc tcgctgccat
     4141 atgaacctta tgaggaggaa tggtgggaat gagaatctct gaaaaagtcg ttcgtatagg
     4201 actcattttg atggttgcgt taagcatata cttctcttat gcaatctggc tgagcccagc
     4261 aggaaaaagt tcaatcaacg tagatgagac aaacactcaa atgatcgagt ctcaaagcaa
     4321 tcgaaaaact tccgaagtct ttcttccgct tcatgttaca tggcaccatt cgggaatgat
     4381 ccaagaaacc aacagtgaaa atctcgtgta ccgattgcaa ttgatcatcg ataaagcaag
     4441 atttggaaag ctttctgaag tagtttcagg agacaaatca cagtttgaac aggtgaaaaa
     4501 tctgaactct ggttttgagt tggcctataa tgcgcctttt ttgctaagag aatacaagga
     4561 agcatttgat ttagatttag atgtgtcgaa cttggcaaaa gaaagcgaaa atatgtattt
     4621 tactagaata caattcgatc aaggaaataa aaaaatccga tttttgaatt attcgaacca
     4681 tctcgtatat gaagcaaata tttcgttgaa ttggacagat atcaataaag aattaacagc
     4741 aaaagacaca aaatggacag aaatgacaac agaaccatcg acgatcgaca cgcaatatgc
     4801 tacgaaacat tcgatcaaac tgaaaaagta tagttatatc ctgtcacagc aaccctatac
     4861 actttttaga aatgcttttt tccagaaacc agatagcgtg aaaaataatg atgaaagcaa
     4921 tgaattgatt ttttacggtg ggaatgaaac aatgtctatc cattcggaaa cgcaagtagt
     4981 tgattttcgt ggagagctgc cagagaaaaa agaaaatgct agtgtatttt ctgaaagttt
     5041 cccctatatt agcaaattag gcagttcttt agggaacctc agatattttg accgcagtga
     5101 caatacgatc gattatcgga tattcgtgga aggcttcccg gtttttggga cagacagcag
     5161 aggacagatt ggtgtggaag ttggcggtga aacgaatggt tctgtgcaag tgaatatcca
     5221 aaccagtttg aatacgattc aagtgccgat accttcagac gaagaagtag aacttgcaag
     5281 tacgaatgaa atcatccagc agttaaaagc taagggagca gatagcaaca agattggttc
     5341 agtgatcgtt ggctatacat ggcaaaatgt caaagaaacg aatcgtgtcg ttgatctctt
     5401 acctgaatgg tatgtgaaat ataaagataa ctggtattca gctaacgacc ttctggaaaa
     5461 attacctgga ttggaggcga attaattgga tttcaaaaaa atcgaatgga tatttttcgt
     5521 cgctttttta gggttgaatg tgtttttatt cagcagttat catgacgcgg tataccaaga
     5581 aaacaatgtc attcgctcta atcagactga atcaatccaa caacgtctgg ctagcgacga
     5641 tatcacttac acggggaaga tttcgtctga gaacaagcaa ggctattatc ttagcgcgga
     5701 gcagaccaat cttagcactt cattagcgaa ttatcgcaag gctggtaaaa gcggcttgct
     5761 ggcaagtggt gtgtttttag acggtgatgt tttgacgaaa tctttatcca gttcagatgc
     5821 tgaaaagaac gtgatcgatc cagataaaat tagtagttcc ttggaagatt ttttatctaa
     5881 caaagacaat attttattcg gtaatgatta tgtgtacacg aaaaactttt ctcatttaga
     5941 agaagaacca ctagaaatcc aagcaagcca agagtatgaa ggcatcccat tcaatgatga
     6001 tacagcaaaa atcgttcttt ctttagaaaa aaacgataat gattatagca tcacgaaata
     6061 catgcagacg catttatctg atatcgagca gctgcgcgaa aagacggaac tgtatacgga
     6121 agaggaagca atcaatacat tgtacatcaa taacaaaata ccaagaggat caaaaatttt
     6181 atggcgtcag ctagcttatt cctgtattct gaaagtccgg gaaaaaaatg tgtatgttcc
     6241 tgtttggcat gtagcgatcg aaacaagcga taaagtggtc caaattgaga gtgtcaacgc
     6301 tttcagcaat acgatcgtga caaacaatac tgtaccaaaa gtggaagatc aataaaaaag
     6361 cgagtataat gaaaaaagtt tacaaagtaa gaagggaagt aggagtcaaa tggctgaaga
     6421 aaaaccagct tttaaaatca gtatcctagc aagtggaagt tcgggtaatt cactttatat
     6481 cgaaagtgaa aaaaaacggc ttttggtcga tgcaggactt agcggtaaaa aaatcacttc
     6541 cttgatggct caaattgatc ggacaccgga ccaattagat ggaatacttg tgacgcatga
     6601 acatcgagat catatacatg gcgtgggtgt cttggctcgt aagtatcatt tggatgtttt
     6661 tgccaatcag cagacttggg aagcgatgga accactgatt ggaaatgtac cagtggaaca
     6721 aaaacatcta tttgagatgg ggaaagtcag aacattcggc gatatcgata ttgagagttt
     6781 cggcgtctcc catgacgcag cggctccgca attttatcgt ttccataaag aaaatcggtc
     6841 attcgtcatg ttgacggaca ccggatattg cagtgattat atgcgaggtg tcattgaaaa
     6901 tgccgatggt tatttgatgg aaagtaatca tgatcttgag atgttacgca tgggacctta
     6961 tccgtggaat ttaaaacagc gtattttagg agatagagga catctttcca acgaagacgg
     7021 tgcacttgtc atgactgatg tgatcggtaa tcggaccaaa cgaatctatc ttggccatct
     7081 tagcaaggaa aacaatatga aagaacttgc tcatttaaca atggaaaatg ttttgaaaga
     7141 aaaagatttt ggcgttggac atgattttga aatctatgat acagatccag atgaagcaac
     7201 tgagctattt tcgatttaaa cagttcaatc aataaaaata taaaaagcag aagataaaat
     7261 caatcagggt tggttttatc ttctattttt atttttttag catattaaaa aaataaccgt
     7321 attatcgact gactacactg atgtgctgct tttcatgttt ctcatccagt atctcatcta
     7381 ctaatgcaat cgcgtaatct gccatgctga tcgtactgtc gccagcagcg tttctttgta
     7441 aataatcatc acttaacaaa taagtacctg tacgtttgcc atgtggatca aataaagaag
     7501 ctggactgat ataagtccaa tgtagatttt tagattgttt caattttaac aatccttcgt
     7561 acatattgaa agctgttgga tagaagggtg catttttagg tacttggtca atcatacgag
     7621 tcgtcttttc tgtatctaag aataacgaag aggcaccccc aacgacaatt aagcgtgtat
     7681 cggttccttc aagcatctca atcaaatggg acaaagaagt ttgatgcatt tcttcttttc
     7741 ctttgggagc gttgaaagca tcaacgacaa catcaaaaga tgcaagatca gtagttgtca
     7801 actcaaataa atccttttgt ataaacggaa catctgatgg caatttttct ggatgacgca
     7861 cgattgccgt cacgtctaaa tctctggaca atgcttcgtc taaaataaat gaacctgcat
     7921 ggccagtagc tccaatgagt gcaattttca taaaaaacct ccttttttat tcctgtccat
     7981 tgtaagcagt tgctgaatat tcgtcaaaga atccgatttt tgaaaagctc agtgcgcatt
     8041 cctttcatgg ttcgtgatca ttgggtatag ttaaattaag aaaactgatt gataaaaata
     8101 gaagttgaag gagataagaa atgaaagagt ggttagatcg tgccttttta ccaatgataa
     8161 catctgttgt gaatcttaga cccttaactg ctttgcgtaa tggaatgact tacacgatcc
     8221 caataattat tgttggctcg ctgtttttct tatttgctca ttttcctgtg gattacatcg
     8281 ctcagtggat agacaaaaca ggattttcta tgtattttac acaagtatac caggctacat
     8341 ttgatgtgat agccttttgg atcgtttttg ccattgcata cgcttatgca aaagaagaag
     8401 acatcgatcg cttaccagtc ggcataacag ccttgtctgc ttatctactg cttattcagc
     8461 cactaaatac tcagatagat actgtctgga caggtgctga agggatacta ggagcagtta
     8521 caactgggct catcatcgga tggggctatg cacgtttagt aagacttgcg aaagggtgga
     8581 agcaaagaga gaagatacct gaaaatgttt ggcagtcttt tacttcattg attccaatca
     8641 ttgttatagt cacttttacg cttatcggtt cagcggtttt tcaacagttg ttcagaatga
     8701 ctgttgttca aggagtatgg acatatgtcc agcttcccat tcaatcgttc atagacacgt
     8761 tgtttggtgt gcttttatta ggtttttttg ttccgttttt atggttattt ggtcttcatg
     8821 gatctgcgat ggtcaatggg ttggttagtc ccgtcttgca agcgaattca ttagcgaatg
     8881 cagaaatctt agcctcagga caagagttga cggtggcaaa tggcggacat atcgttactc
     8941 agcaatttct cgatcagttc atgacggtta cgggtgctgg attgacgtta ggggctgtat
     9001 tttttatgac ggtttttgcg aaatcgaaga aatacagaga attaggaaat cttgctcttt
     9061 taccagcctt tttcaacatc aatgaatcaa ttattttttc cacaccgatc gtgatgaacc
     9121 cgatgatggc tgttcctttt attttcgcac cggtcgtttc aggattgatt acctattccg
     9181 ctttatattt tggattcgtt cctttattta cagcagtcca agtcccttgg acaacaccgc
     9241 cgatcctctc gggtttctta gtaggcggag tgcctgcagc gattttacaa ggtatcgtgt
     9301 tgtcaattag ttttttcatt tatttcccat ttttgaaaaa aatagattct gcaaactgca
     9361 aaaaggaaag acagacaaaa aatgatggag cagcagaaaa gatgaacgac taaaaaacaa
     9421 aaatactcct gtcgctttag attgggaaaa tcaccaatct aggatgacag gagtatttat
     9481 agtcatgttt atactagccc tttttggaca gctaattgaa caagaacaac taatgtgttc
     9541 attcctacgt gtgtgatcat cgatgtccag atccgaccag ttgctttgta ctctaggctg
     9601 aagaagaaac ctaggaaaaa gtagattagc agatgaccat cattatgagc aaacgcaaac
     9661 agcaaagagc tgatcactgc tccgacccaa aaatttgaat atcgactgat gatccccagt
     9721 atactgcggc ggaatacgaa ctcttccatg atgggtccag caacagttgt cgccaaaata
     9781 aaagcgggtg cttccatgat catctggatg atattttgtg tattctcgga aggggttgct
     9841 ttgccaaaca atacagattc taacatgaca gcgatgcttt gcagaatcaa tgcgacaaag
     9901 attccaacca atccataaat aacaatcttt ggagcagaag ctttttttac cgctgattcg
     9961 atcgctaagg gttcacgttg cttaaaataa aaaatagcta ggacaacggc gcctagtata
    10021 tacatgactg cagttgctgt gatcaattca gacgtagatt ttacaagtcc tatcgaagct
    10081 aggatcaagg gtgagataaa aattagacca tagcaaaaaa tgctgaggaa gctatatttt
    10141 tttaaagaca tgtaattcct ccttttaagg gataacaagc atgcaaggca agcttatatc
    10201 gattgatttc aattatatca taatttcaat aagcagctgt cagaataaaa tttcttttct
    10261 ttgatttaaa agcttgcaaa aaagagggag aatgagtatt attaaaaatg taggttagca
    10321 ctcgaaggtt aagagtgcta acagattaaa aaaagagtta tcggagggat ttatcgtgtt
    10381 aaaaccatta ggtgatcgcg tcattatcga agtcgcaaaa gaagaagaaa aaactgtcgg
    10441 aggaatcgtt ttagcttctg ctgctaaaga gaaaccccaa actgggacag ttgttgcagt
    10501 cggtgaaggc cgtttgttag aaaacggtga aaaagttcct gccgctgtaa aagcaggcga
    10561 ccaagttatg ttcgaaaaat acgccggtac agaagtaaaa tacgaaggaa aagaatatct
    10621 gatcgtcgca ggaaaagata tcatggcgat tgttgagtaa atttggaaga taaaaaaata
    10681 atcaaaaata atcaatgagg tgaatcaatc atggcaaaag aattgaaatt tgcagaagac
    10741 gcacgcgcag ctatgttacg cggggtagat aaattagcag atacagtgaa agtaacatta
    10801 ggacctaaag gccgaaatgt cgtactagaa aaatcttacg gctctccatt gatcacaaat
    10861 gatggtgtga cgatcgcaaa agaaatcgaa ttagaagatc attttgaaaa catgggtgca
    10921 aaacttgtat cagaagtagc atcaaaaaca aatgatattg caggagacgg tacaacgaca
    10981 gcaactgttc tgacacaagc aatcgttcgc gaaggtttaa aaaacgtaac tgcaggtgct
    11041 aatcctctag gcattcgtcg cgggatcgaa ttggcaacaa aagcagcagt cgaagaattg
    11101 cataatatct caactgttgt tgattcaaaa gaagccattg cacaagtagc agctgtttct
    11161 tcgggttctg ataaagttgg acatttgatt gctgatgcaa tggaaaaagt tggaaacgac
    11221 ggtgtcatca caatcgaaga atcaaaaggg atcgaaacag aactagatgt tgtagaaggt
    11281 atgcagtttg atcgtggtta cttatcacaa tatatggtaa cagataacga caaaatggaa
    11341 gctgttctag aaaacccata tatcttgatc acagacaaaa aaatctctaa catccaagat
    11401 attctgccat tgttagaaca aatcttgcaa caatcacgtc cattgttgat tattgctgac
    11461 gatgtagatg gtgaagcatt gccaactctt gtattgaaca aaatccgtgg aacattcaac
    11521 gtagtagctg taaaagctcc tggtttcggc gatcgtcgta aagcaatgct tgaagatatc
    11581 gctattttga caggtggtac agtaattact gacgatctag gtttagaatt gaaagatgta
    11641 acaatcgaaa acttaggaaa tgcttctaaa gttgttgtag ataaagacaa tacaacgatc
    11701 gtagaaggtt ctggagaaaa agaagcaatc gaagctcgtg tgcaattgat caaaaaccaa
    11761 attgctgaaa caacttctga ttttgaccgc gaaaaattac aagaacgctt agctaaatta
    11821 gcaggtggtg tagctgttgt taaagttggt gcagctactg aaacagaatt gaaagaatta
    11881 aaattacgca ttgaagatgc attgaatgct acacgtgccg cagtagaaga aggaatggtc
    11941 tcaggtggtg gtacagctct agtgaacgtt atcagcaaag tttctgctgt agaagcagaa
    12001 ggagacgtgg caacaggtat caagatcgtt gttcgtgctt tagaagaacc aattcgtcaa
    12061 atcgctgaaa acgctggtta tgaaggatca gtgattgttg ataaattgaa gaacgtggaa
    12121 ctaggcactg gtttcaatgc tgctactgga gaatgggtaa acatggttga agcaggtatc
    12181 gtagacccaa ctaaagtaac acgttctgcc ttgcaaaatg ccgcttccgt ttctgccttg
    12241 ttattaacga cagaagcagt cgttgcagat aaaccagaac cagcagcgcc agcagctcct
    12301 gcaatggatc catcaatgat gggcggtatg atgtaagaaa aagcaacttg cttatctaca
    12361 actgatcatt agtgaaagac agataaaaag aggctggaac agaagcgttt aactccaaga
    12421 aataagaaga aattcacgga aattgctttt caaatttttg tgaatttcag cttatttccg
    12481 aaggagttgc ttctgcttcc gctgtttata cgtgtttaga gcgtgagaca aaagtgttct
    12541 ttacttt
//
LOCUS       DAIGOK010000084        12129 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.084, whole genome shotgun sequence.
ACCESSION   DAIGOK010000084 DAIGOK010000000
VERSION     DAIGOK010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.084"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(<1..142)
                     /locus_tag="NOA01_001894"
     CDS             complement(<1..142)
                     /locus_tag="NOA01_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCI0633080.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHN"
     gene            complement(139..792)
                     /locus_tag="NOA01_001895"
     CDS             complement(139..792)
                     /locus_tag="NOA01_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCI0633081.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1080..1600
                     /locus_tag="NOA01_001896"
                     /pseudo
     CDS             1080..1600
                     /locus_tag="NOA01_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1502..2218)
                     /locus_tag="NOA01_001897"
     CDS             complement(1502..2218)
                     /locus_tag="NOA01_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HCI0633082.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(2268..2477)
                     /locus_tag="NOA01_001898"
     CDS             complement(2268..2477)
                     /locus_tag="NOA01_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633083.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            2754..4027
                     /locus_tag="NOA01_001899"
                     /pseudo
     CDS             2754..4027
                     /locus_tag="NOA01_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(4134..5165)
                     /locus_tag="NOA01_001900"
     CDS             complement(4134..5165)
                     /locus_tag="NOA01_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0633084.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(5172..6002)
                     /locus_tag="NOA01_001901"
     CDS             complement(5172..6002)
                     /locus_tag="NOA01_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI0633085.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(5999..6814)
                     /locus_tag="NOA01_001902"
     CDS             complement(5999..6814)
                     /locus_tag="NOA01_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCI0633086.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(6811..7635)
                     /locus_tag="NOA01_001903"
     CDS             complement(6811..7635)
                     /locus_tag="NOA01_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HCI0633087.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(7625..8725)
                     /locus_tag="NOA01_001904"
     CDS             complement(7625..8725)
                     /locus_tag="NOA01_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCI0633088.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            8903..9688
                     /locus_tag="NOA01_001905"
     CDS             8903..9688
                     /locus_tag="NOA01_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HCI0633089.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            9721..10104
                     /locus_tag="NOA01_001906"
     CDS             9721..10104
                     /locus_tag="NOA01_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCI0633090.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            10180..10311
                     /locus_tag="NOA01_001907"
     CDS             10180..10311
                     /locus_tag="NOA01_001907"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCI0633091.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            10280..10516
                     /locus_tag="NOA01_001908"
     CDS             10280..10516
                     /locus_tag="NOA01_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCI0633092.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(10594..11565)
                     /locus_tag="NOA01_001909"
     CDS             complement(10594..11565)
                     /locus_tag="NOA01_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCI0633093.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         3644 a         2747 c         2219 g         3519 t
ORIGIN      
        1 tattatgaat cttttctttt ctcgtctcca aatatgtttt aggaaagata aaaaaaatta
       61 atgaatgaac taatataatt attattccaa gaacagaaaa tatttgtatg aacatttttg
      121 gaaatatctt taattttttc attttctctc caatttatat cctacatttc ttatagtagt
      181 tatacagtct aattgtaatt tttttctaag ttcttttata tatacatcta ttactcgatc
      241 ataaggagtt tcttcgctat ctttccatac ataatcaata atttgcattc ttgttaacac
      301 ttgtccatta ttatccaata aacattttaa tacttctagt tcttttgcat ttacatctat
      361 ttcttcacca tttattttag ctgtatagct attaaaatta accgatagat ccttatattc
      421 aaacttctct aaatgtccat aattcttctt aattaaagaa tctattctag cttttaagac
      481 cggcaatgaa aatggctttt ctacatatcc gtctactaaa ttagtaaatg catcaatttt
      541 atattcttca tcactaaatg cagtcaagat tagaattggt aaattgcttt tctttctaat
      601 ttctttcaac acttctaaac catttataaa aggtatctga atgtctaaga taaccaaatt
      661 tatatcgcta ttaaattttg acaaggcttc tcttccatct ttagcttgaa taacagtata
      721 tccaaattct gaaagatatt cacttattcc ctctcttatc atattatcat cttcaacagt
      781 taatattttc atatatacct ccacttaagc ctatacacta gatattatac agcatattat
      841 ggctctataa tatctagtgt tggtgacgcc ttctgttgcc aatacttttt ctttttttta
      901 ataaatgcaa acagacacct tgaaatcctt tagaaaaaag gcttctataa tattttgtgt
      961 tgttggaaaa tcgaaggcga ttttctacta acactttttc ttcgtttcaa ctcaaaaaga
     1021 gactcacttt tggtaaaatt tagtcgacca aaacatcacc ctaccaaagg agtcgctcta
     1081 tggataatca tactagaaaa ttactcggat taacagataa acatttattt tttgatgaag
     1141 aatggctgat tgaaaaagaa tataaaggcg ttcaagcgca gtttattaaa gggagattgg
     1201 atgactcacc ttcccattgt gaacactgtg gatcgattcg tattatccgc aacggttcct
     1261 atacgacacg tactcagatg ttgaaagtca aagaaaagtt gactatttta gaattaaaac
     1321 gtacccgttt tctatgctat gattgtgggc agaccttttc tgcgaaaacg gacttagttg
     1381 atgaacatca tcaactaaca aaggaactga agcaggcgat tctcatagaa ctgtacgaaa
     1441 atcaatcacg taatctgaaa atcagaagaa gtaccgtcgg ctcaagcgat attggaaact
     1501 cttactcaaa gattccacga cattggagcc gctcaaacgg cattatcatc gactgtttaa
     1561 acgaccaatt agtcaaacag aaatcgtcga tgaaccgttg tgcacaatgg tgtcctgaac
     1621 gtacagcgac accttccata tccagagcag tcgccacatc acggggatgg atgccatcca
     1681 aattaaaagc aaccacgcca gtatgatgtt caggatcttg cggtccgtaa atggtgatac
     1741 cgccgatcgc ttgtaattta ggtaaaacgt aggcaaccag ttcttgctca taacgatgaa
     1801 tattctccat accaatctca ttcaagtaat cgattgctcc tgcaatgttc ggagtaccag
     1861 cttcaaattt ccatggcagc tctttccatg aactatcgta aagattgaca aaatctatca
     1921 tttcaccgcc gaattcgact ggctccattt gttctaacaa ttgacgtttg ccatataata
     1981 caccgattcc tgtcggcccg cacattttat gcccgctaaa ggcgaaaaaa tctgcatcca
     2041 acgcttgtac atcaactgtc atatgcggtg ctgcttgggc accgactgga ctatcctcaa
     2101 ctacaacagc ctgttcaacc ctcagccgtt tcaagcacaa caaataacct tctggatctg
     2161 gtttgccgac ctgaagatca tcgatcgtca caaaggaatc aattttttcc ttaatcatcg
     2221 gatgtgcttc aaaaaaatag tctagctccc gcagttcaga attgagacta ctccgatttt
     2281 ccagtgctgc tgatccgctg ctcaaaatgt tcagcatcca ctggtctccc aaccgcttga
     2341 cttatccctt cataaaagga cagttctgct gccgtcccaa caccttgttg atagtctgca
     2401 aaaggaattt tatagccgaa ctgctccagt aaataggctt cagtaaatag tgatgaatac
     2461 tctcgtaaaa catccattca ctgtcaatca tcgtcccatc aaaatcaaaa ataagtgctt
     2521 tggtcatctt ctttccccct tacccaaaac aagcaaaagc tgtgtcctgc tctctctaca
     2581 gtatcagcat aaaaactgcc gtctgaaaag tcaaaggtga aagaaatgtg gcgctataat
     2641 ttctaggact gatagtaaat cattctatca gtccttttga gcataaaaaa acaaaacata
     2701 cagaagctcc gttagaattg aatcaccaca acacaatcaa aggagcgttc tgtatgcctg
     2761 atcttcatct tatcaaacta ttactaggaa tcaaagatgg aaacattcaa atcactcatg
     2821 tcgagaagaa aaaaatacat tcaatcaaca gtatcgttgt ttctgccacg ctcattaaaa
     2881 cgattcagcc ctgtcctcag tgtaaacagg tgaatcaaca tgggctgatc gttaagaacg
     2941 gaaagaaaaa atcgctggtc cttctcaata agtgtgcgaa tcaacggact tatcttgctt
     3001 tggcaaagca acggtacctc tgtcgtggct gtcacaccta ttttacggcg aatacctaca
     3061 ttgttgaaaa aaactgtttt atcgccaaac aagtacatta taaaatattg gaggaattga
     3121 ctgaaaaaca agcaatgacg acgatttcta aacattgcgg cgtttcttgg tcaaccgttt
     3181 cgcgaacact cgcttctctg ctccccatga caaacgtgaa aataaattgg ttgcctcgct
     3241 gttcattggt agatgaattt cgttcattga aaaatcaagt cggtccctat tctttttctt
     3301 gtatggatgg cgatactggg aaattactgg atattcttcc ttcacgtaag aagaaagatc
     3361 tcatttctta ttttatgcaa tttgaacgac gtgcacgttt aaacgtaaaa atactcgtaa
     3421 cagatatgaa cgcctcttat gctttgctga taaaagagtg ctttcctcat gccaaactcg
     3481 ttgtggatcg ctttcacatc gtcaagcacc tcattcgcag ttttaaggat atttgactat
     3541 gagtcatgaa aagcttcgac cgaaacgatc caatacaagc caaacactat cgacaagtga
     3601 aggctttatc tcgacttctg atcaaacgac aggacacgct tgtttatgat aaatggacaa
     3661 aatggcgtaa ttttggttgg gcctatttaa ccgaaagtga agtcgtagaa cgtctacttt
     3721 ctacttctga tgaactacga atagcttatg cttactatca agaaattctt caagcttttt
     3781 atgacaaaga agcggacatc ttctttcagc ttgtaaaaac aatgcccaag tcggttccaa
     3841 gagaactgca tcatataaaa aaagcgttta taaactatga atccggcatt cgtttagcct
     3901 tagagttgcc ttactcaaat ggcaaaatag aaaatctgca tacccacatc aaagcattga
     3961 aacgaatagc ttacgggtta ggatgagaac cagaattttc ttaatcaatc atctgatcac
     4021 ttattaaaca taaaaaaaca tctaaagcag aaaatcaatt ctaccttaga tgttcttata
     4081 tcactctatc agtcctattt gacaaagagc cagaaatgtt tgccgactat acactagctt
     4141 tgttgtgcaa aatgctgagt atcaatttcc acagggattg ctagaatatc tttcagctga
     4201 atgtataagg catcgctaat tttctcttcc cgatcgttca accgatccac taaaatttct
     4261 ttgccctcca gcgtttgaaa aacataccgt tgaatgttgc ctaaaataaa tgattccttc
     4321 catagaaccg gaatcttaac cccctgagga accggttcaa attgaatcag ctccggtctt
     4381 aaataataaa tgcagcgatt gcccttagcg gaaaactcgt gggtatactg attcagctca
     4441 taccattcga aacgattata gttgccgata aattccgcaa caaatcggct ctgaggattc
     4501 tgatacaatt ccttcgaggt ggaaacctga gccacccgcc cagcttccat cacaaaaatc
     4561 cgatcactga ttcgcatcgc ctcttcttgg tcatgagtca caaaaatcat cgtcatccct
     4621 agcgattgct ggatagcgcg caaatcccgc tgcagctgtt ttctaattcg ggcatccaaa
     4681 gcacttaatg gctcatccag cagcaggact ttcggttcag tgaccaatga gcgagccagt
     4741 gccacccgtt gtcgttgacc gccagaaaga aactggacat ttttatctgc ctgctccgtc
     4801 atatccacca atgatagcat ctcaaaaacc ttttcctgaa tcacctcagg actcagcttc
     4861 tttaccttca aaccaaaggc tacattttca aaaacagtca tagttggaaa caatgcatac
     4921 tgttgaaaaa caaagccgat attgcgatcc ttcgtaggct tatcggcaat attctcattg
     4981 tccagcacaa tctcaccgct ctttggttgt tccaatcccg tgatacagcg cagcaacgta
     5041 gactttccgc acccgctggg acctagtaaa gtaaccagct cacccttttc aatctgaatt
     5101 tcatcaatag ctaaaatctg tttttcatca aaaaacagat tgagatcatg tatttctaaa
     5161 aacattttcc gtcacaactc ctttaagggc ttctttttcg actgcttctc ttccctcgtc
     5221 tgcttaaggg acagctgttg cttttgtcga taagtcagca taaaggtagt taaagcaatt
     5281 cccaccataa acagcaaata caaaaccatc acagcgctag ccaaatggcc gttttcgttc
     5341 atccgccgca gcatatagat gcgtaccgtc tgaaatcgac cgccaatcaa caggttcgtc
     5401 agcatgtact ccccaaaaac cgaagaaaaa atcatcagag aaactagggt cacaccgata
     5461 cggatgtttg gaaacagcac ctggataata atcgtagaca tcggggcgcc caaggtttct
     5521 gctgcatcca ctaattcctt cagattaatt aagcgcatct ggttgttaag actaagatag
     5581 acaatcggca acgccgtaat aaacaatgca cctaccaaca ctacaaacat tgggatacca
     5641 gtttcagcat acgttcgaag caaagccgtt accaaaatca ctcctggtaa agcatacggc
     5701 agcaggagca gcttttccag ccaacgattc aaacgaggga aatacaaatg aatccaaata
     5761 atggtgggaa tcatcaacaa cagaatcgtc accaagacca cacctgctaa taaaagtgag
     5821 cgacccaccg cagcaataaa ttccctgtcc gtcagcagct gttggaacca ttgcaaggta
     5881 aaatcactag gtaaaagcga ttttgaccat ttagtggagc tggcatacaa aatcgttcca
     5941 ataatcggca gcaagtaata caggacaaag cagaacaaaa tcggttttat taactttttc
     6001 acgtctgcac caccttgcct ttcttttgaa taatcaacat tgccgttacc agcaggacac
     6061 ctaaagcgac tgccaacgca ctgcctaaat taggcttggc gaacacatct cccgacacta
     6121 aagccgcaat tcgaatcgtt aaaagattta cattactgct ggtcagggca tacgctgttt
     6181 cataggtccc catgccatta gcaaataaga tgacaaaggt gctggcaatc gatggtcgca
     6241 aaaaaggcaa agccaccttg aaccaagcat acgctgatga agcgcccagc aattgtgcgg
     6301 actccagcca ttcctttttc acttccttca tgctgggata caaaaacaag acccctaacg
     6361 gaatttggaa ataggtatac gtgatggtca atccccacca agaatacaaa ttgaagtccg
     6421 ctagaaatgg caaaatcatt ttcagcacac ctacattccc caacaaaata ataaaagaga
     6481 atgccagcgg aatcccagca aaattagctg ccaaattgga aacaaaggtg attttctcct
     6541 gcaatttagt cgatagattc attaagcata aacataaaat aaacgtcccg attaacccaa
     6601 tcacactgga gctgacagca atcagtacgc tgttaacaaa ggcctgtgtg taatatttgt
     6661 tgctgaaaat atcactgtaa ttggccaatg tccactggtt agttaaatca ctgtgcaagc
     6721 tgcacagaac cataaaaagc agcggcagca gtaaaaatgc acagaagata aaggaggcag
     6781 gaaccagaac caagcgtttt ttcatctctt tcatcccctc atcaattctt gaatgtcctg
     6841 cattttctct gacggtgcaa ttcctaataa ccagcaaaac aatggagccg ccagcagctg
     6901 tggtacagtt tgttccttca gcttttggcg tttatttgaa atcaaataaa aaggaacttc
     6961 acgatgtgca gccaagctgc cgccatgcag tccgtgcgca tccatcccgt gatccgcagt
     7021 caccacgact tgataacctt tttgcagcca ttcaggaagg taaatagcca ataatctgtc
     7081 cgcctgatta accgctgctg catattcatg agaatcggcg gtaaatttgt gaccgacatc
     7141 atcaatattc atcgaatgta cgaccaaaaa gtctggctga tactgattca ctaaataatg
     7201 ggcatccgca aataaatgcg aatcagggta agtgtcctca ctataaaaga tgccatgctg
     7261 aatcagtcgc tcctcctgat gctggatgcg atcaaccaaa cgattatagg gggcttgatt
     7321 gtacaactcg ctgtaccagt ggtaagcggc ggctcctgtt tttccgccag cttccttaac
     7381 taattcaaac agcgaccact ctttggatcg ctgagcatgg taattactat agatgccatt
     7441 ttcataggct ggaacgccgg tcatcagtac ttcgtataac ggacgtgaat tgctgggcag
     7501 ctcacttttt accttcaata agcttgcttg ctggtgctca actaaatgat tcatgaaccc
     7561 aagctgggcg acggctgtgt cgtagcgaca gccgtctagt ataactacaa tcagtttgtc
     7621 aagtctactt tgcataggtc agtacctccg cctgccaaag ctctggaagc tctacagagg
     7681 tcttgttcca agcttcattg tcttcaactg gcaccgccgc ttcataagca tcctccggca
     7741 gtaatttttc agctacttct ttaggcagct ctaccttgcg aataggtcgc gcatagccct
     7801 ttgccaaatt gatttgccct tcatcagata aaatatattc ccgtgctagc tttgccgcat
     7861 tgggatgagg ggcattttta ttgattaagg ttgtataccc gctaatgatg gagccgtctg
     7921 ttggcacagt tacctcaaaa cgctcggagt caatttggtc acgataattt agtccattga
     7981 agtcccaaac aactgccaca tcgatttcac ctttttcaag atttgctacg ctggcatcaa
     8041 cggtggacaa acggcctttt tctgccattt ttttgtagta attcagacct ggctgaatat
     8101 ccttttcgtc acctccgttg gcaatcgccg cagccaatac agcaaattgt gcttggttgg
     8161 caatcgtcac atcccccacg ctaacttgat agtcaccctc ttgcagctcc ttccagctag
     8221 tcggtgcttg cttcacattc tctttatcgg tgataaagga aatggttccc gtatagctta
     8281 acaaccaatc cccatcatca tcctttgccc aatccggaat ttcctcccaa tagttggtct
     8341 tataaggtaa ggttaactct ttgctttcag ccaacggacc gaagctgata ccgacatcac
     8401 caatatcggc ggttccattt tttccttctg attcaaactt agccaattct tctgcactgg
     8461 acatatccgt atccacgtga cttaagccgt attctttttc aaggtcgttc caagtgccta
     8521 cccaatttgc ccaagtatcc ggcattccca agctggcaat ctctccttct tccttggctt
     8581 tttctataat cgtatctaaa gagtcgtctg ccgcagccga ttgcttcgtt gcttcacctg
     8641 agccgcagcc tgtcagcagg atacttactg ttgctgcaca aaataaaccg atagtcatgt
     8701 tacgaatttt ttcattcatt ttcattcatt cttctcccct ttcgataatt ccattctaac
     8761 aaagctttgt aatgagagaa tcagtttctt gaaaaggttt catgaacaat gtaaaggttg
     8821 tatatggttt gtaaatttcg acgaattccc gttaaccttc taaagctttt gcggtatgat
     8881 agtagaaaaa aggaggctcc ctatgcagta ttatgatcag catctccaca cgtatttttc
     8941 ctttgattca gaggaaaaat ttgaaaacta tttagcctat cagccggagt ttttcgtttc
     9001 tacggatcac tttgatttaa acaatccatc taccggaaaa gatgacattc cggattatcc
     9061 agcctatata aacaaactgg actctttaaa agaaacctat tccaccaaat ttctgcgagg
     9121 tatcgagatt ggcgttgttc caggacaaga agagcggatc gtggattacc tcgctcagca
     9181 tccttatgat ttaaagctgg taagtattca tcaaaatggc cgctttgatt atatggacga
     9241 tgtggtttta accaaagatc ccattcaaac taccaaggaa tattttgatc agatgttgca
     9301 ggtcttgacg gattttccag aaggagatat tttgactcac tttgattatg gattacgccg
     9361 cttcaccttc acagcggaag aattcgcgga gcattttgag gctcagctgc taacgatttt
     9421 caaaaaggtt gttgaacgag aattggcgat ggagttaaat gccaagagct tcttgaaata
     9481 cggcaacgaa gacctttacc gttatgccgt tcctttgtat caaagtgtcg gcggaaagct
     9541 ctttacctta ggctcggatg ctcatgttgc cgaagattat cagctgggct ttaaaaaaat
     9601 gagtcagctg ttggcagact gtcaggtaga aaagttgcta gtcattcaag gaaaagaacg
     9661 ccgcctgacg ccgctgccga ttttgtgatt cctatataat agaagaaagt ggtgaaaaaa
     9721 atgagcaacc tgtgggacta caatcaagag gcacccatcc attatttaat tgcgcgacat
     9781 tgggatgcgt tgaaaattga ggcggtttgc cgatccttgt tagcagccgt tccaaaacaa
     9841 caactggaaa attttttggt ggctgacagc ttgcagcggg aaaaagttca ggcttatttt
     9901 gcagccttta aagatcagcc tttggaatat ttgcatgcgc agtttcatct tttttatcaa
     9961 gtagccgccc cagatgacta caacgattta cgaggacagc tgcaattgac ctttcaagcg
    10021 gatgaaaccg cctataccgt tttattggga atggctcgcc ttggtgatca ggcgaaggtc
    10081 gagtggcgga ttttcgatat ttgatggcag gatcaaggcg tttcagttgc aaaccttcct
    10141 ctctttcaat tagaaaaacc gcagcaggaa gaagtttgtg tgctggtcac cgatgctggt
    10201 gaaattcgtc tccgcttatt tcctgaccaa gctcccctgg ctgtagccaa ctggcgctcc
    10261 ctagctgagc aaggcgttta tgacaacaca cctttcggct cgtgtcatta aggaatttgt
    10321 tattcaaggt ggcgctttgg atggttctgg tgatgaagct gtttccagct atgagggctt
    10381 tttcgctgat gaagtgcatc gcggacttta tcattttaat ggcgccttgg ccttagggga
    10441 ccacggcccc cacaccaacg gcaaccagtt ttttattgtg caaaacacaa aggcgcaggc
    10501 tgacctgcta atgtaaagca agcatacttg gagcttggag gactaccgga gctggatggt
    10561 cgttatactg tttttggaca ggtataccaa gggttagaaa tcgtagcagc gatcgcagct
    10621 cagccgacaa acagtgaaga tgtaccgatt gagccagtgt tcattcgaca aattaacttc
    10681 gaaaaaacat cgtaaaaaga cactcactga cttcagcaaa agctctattc ttgagctggc
    10741 tgcagctagt gagtgtcttt catttatcca taagataaag cttggattca ataaataaat
    10801 aaatgttttg gttaaagctg tgaccagcaa atgaggaacc gtgcgagcca gttgatctgc
    10861 caatgcaaac atttctttag gtgaaggcgc tggcgtgtct gccaacggat actccagtcc
    10921 caatgcttgg taaccgtgca taccatagca gtggtacggt agtaattcta acggtacgaa
    10981 ccgatcaaga tctgccacaa aacaagcaat cgtcagcagc tcggtcaggt tcatattgat
    11041 acctggaacc acaggaactc gaattcgcac ttcgcagtta ggatactcaa ttaaaaagcg
    11101 acgaatattc ttcaaaattt gttggttatc taagccggtc cattgtttgt ggagcttgct
    11161 ggtaaacatt tttacatcca caaacaatgt atccacatag ggagctacct tttggatttc
    11221 attataagca ccacaaaagc tggtttccag tacggtattg attccaatgt atttcgattt
    11281 ttgcaaaatt tctttagcaa aatccgcctg aaccagtgcc tcgcctccgc taatggtcac
    11341 tccgccatcc gagtgaaagt aaaaaacagc atccttctca atctcatcca ttacctcttc
    11401 agcggtcatg gtctggccaa atcctttttc aatctgcttg ctttgtgatt cgggaatcga
    11461 acaccaggca cagcgtaatg ggcagccctt taaataaaca acggtccgta aaccagcacc
    11521 atcatgtaga gaaccctgtt ctattcttaa tactgttcct tccatataga tttttgaatt
    11581 acacactagt ttaaactaaa acacacaagg aaaaacttac catttaatca taaaattagc
    11641 tcatataaat atactaggat gtaaaataga ctggtcaaaa tctggtcaaa aaagaggact
    11701 aatacaggta gtcctctttt ttgttatttt aatattctaa atcatttttt attggtttat
    11761 tcccttattc gatgtagact caaatcacta tatcactaga tatcaaacgg cgtcatacgg
    11821 aatcaaaaac atcaaatttg acctgttaaa tggtcattga ataacattca aaatcaataa
    11881 aacggagtca gacacggagt caaaaacgct gagagtaaaa aatatataca gccttgtagg
    11941 taaagtacca cagggctgta tattttttat ttcagattac cttaaatgat tccatttact
    12001 ttttatctca ttttttccta ctagttagta tcaaatactc tcctattttc tcaataaact
    12061 atttttggat acatggtatc tgaaacagct aaaaccttgt cattacagca aaaaatcacg
    12121 ttatccaga
//
LOCUS       DAIGOK010000085        12070 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.085, whole genome shotgun sequence.
ACCESSION   DAIGOK010000085 DAIGOK010000000
VERSION     DAIGOK010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.085"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(21..578)
                     /gene="frr"
                     /locus_tag="NOA01_001910"
     CDS             complement(21..578)
                     /gene="frr"
                     /locus_tag="NOA01_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCI0633094.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(581..1303)
                     /locus_tag="NOA01_001911"
     CDS             complement(581..1303)
                     /locus_tag="NOA01_001911"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCI0633095.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1439..2320)
                     /locus_tag="NOA01_001912"
     CDS             complement(1439..2320)
                     /locus_tag="NOA01_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCI0633096.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2419..3201)
                     /gene="rpsB"
                     /locus_tag="NOA01_001913"
     CDS             complement(2419..3201)
                     /gene="rpsB"
                     /locus_tag="NOA01_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCI0633097.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3560..4039
                     /locus_tag="NOA01_001914"
     CDS             3560..4039
                     /locus_tag="NOA01_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HCI0633098.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4256..5347
                     /gene="phnW"
                     /locus_tag="NOA01_001915"
     CDS             4256..5347
                     /gene="phnW"
                     /locus_tag="NOA01_001915"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HCI0633099.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5340..6125
                     /locus_tag="NOA01_001916"
     CDS             5340..6125
                     /locus_tag="NOA01_001916"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HCI0633100.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(6471..8162)
                     /locus_tag="NOA01_001917"
     CDS             complement(6471..8162)
                     /locus_tag="NOA01_001917"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCI0633101.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8584..9531)
                     /gene="arcC"
                     /locus_tag="NOA01_001918"
     CDS             complement(8584..9531)
                     /gene="arcC"
                     /locus_tag="NOA01_001918"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCI0633102.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9646..10665)
                     /gene="argF"
                     /locus_tag="NOA01_001919"
     CDS             complement(9646..10665)
                     /gene="argF"
                     /locus_tag="NOA01_001919"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCI0633103.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10756..11985)
                     /gene="arcA"
                     /locus_tag="NOA01_001920"
     CDS             complement(10756..11985)
                     /gene="arcA"
                     /locus_tag="NOA01_001920"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCI0633104.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT         3296 a         2520 c         2178 g         4076 t
ORIGIN      
        1 cctctgtttt tatgatttta ttaaacttcc aataattctt tttctttatc agcagcgatc
       61 ttatctactt ctttaatgct gttatctgtc aatgtttgta cgtctttttc taatccgcgc
      121 aaatcatctt cggtaatatc accatttttt tgttgttttt tatattcatc gatcgcatca
      181 cgacggatgt tacgaatcgc gatttttgca ttttctgctt cttttttcac atctttagct
      241 aattcttttc tgcgttcttc tgtcagttgc gggataacta agcgaatgac atttccatca
      301 ttggttggac tgattccgat atcacttgcc ataatagctt tttcaacatc ttgaagaatg
      361 ctcttgtcaa aaggtgtgat cattaaaaca cgtgcttctg gaatttggat tgatgccatt
      421 tggttcaatg gtgttggcgc accgtagtag ttcacagtaa tacggtccaa aaggctagcg
      481 ttcgcacggc ctgcgcggat ttgcccaagt tcacgttgaa gattttgtgc tgcttttttc
      541 attttttctt ttgcttctgt cataattgca tctgccattt ttatttcccc cttacagttg
      601 ttccgatatt ttcgcctaag attgcacggc gaatatttcc gtgttcattt aaattgaaga
      661 caagcagtgg aatatcatta tccatgctta gtgaacttgc agttgaatcc atgacttgta
      721 atcctttggc aataacttcc agatgagtca gttcttcaaa ttttactgca tttgcatcta
      781 ctttcggatc agcagaataa acaccgtcta cattgttttt agccatcagg ataacatctg
      841 cgccgatttc tgccgctcgt aatgctgctg ttgtgtctgt cgagaagtac gggttgccgg
      901 taccgcctgc aaagataacg atccgtcctt tttctaaatg acgttctgct ttacggcgaa
      961 tatatggctc tgcgatttga cgcatttcaa tcgaagtttg cacgcgtgtc ggtacaccca
     1021 cgttttccaa cgtatcctgt aaagctaagg cattcataac tgtagctaac atacccatgt
     1081 aatcagcttg agcacgttcc atgcccattt gtgctccgat ttgtccgcgc cagatatttc
     1141 cgccacccac aacgattgcc atttcaacgc ccagctcgtg aacttctttg atttcttgga
     1201 tgatttcttt gattactggt ggtttgattc caaatcctgc atccccagct aaagcttctc
     1261 cactcaattt taagactaca cgctgatatt tcggtgtgac catgttcatt cctccaatat
     1321 tttctattgc cattttacca tacttagatg atttcgacac tctaagactg gtttttcttg
     1381 ttttgcttaa aaaaagggag cgcacaaacc gtgcgttccc gtctgttatt caacagaatt
     1441 attttttcac ttggctcata acttcttcta caaagttatc ttcgcgtttt tcgatacctt
     1501 cgccaacttc gaaacgtacg aatgttttaa cagttgcgcc ttttgatgca acgtattttt
     1561 caacagtcat atcaggatct ttaacgaatg gttggtccac caaagcgatt tctgctttga
     1621 atttcttcaa gcgtccttct accatttttt caacgatatt cgctggtttg ccttcgttca
     1681 atgcttgttc tgtaagaaca gttttttcat gttctaattc agcttctgga atttgtgatt
     1741 cgtttacata acgaggattg atagctgcta cgtgcatagc tacgtctctt gcaactgttt
     1801 cgtcagttgt tccatctaga acagtcaata cggcaatacg tccgcccata tgcaagtatc
     1861 caccaaatgc agcattgtcg tctttttcta caacttcaaa gcgacggaag ctgatttttt
     1921 ctccgataac ttgagttgct tcgatcaagt cagattcaat tgtaccttta tctgttttga
     1981 ttttcattgc agcttccatg tctgctggtt tgttttcagc aacaagttct gcaatttctt
     2041 ttactaaatc ttggaacatt tcgtttttag aaacaaagtc tgtttctgag tttacttcaa
     2101 cgattgctgc agtgttacct ttcacagcaa ctgaagccaa tccttcagct gcaatgcggt
     2161 catttttctt cgcagccttt gccattcctt tttcacgtaa aagatcaact gctttttcca
     2221 tgtcgccttc tacttcaact aatgcttttt tcgcatccat cattcctacg ccagtcattt
     2281 cgcgtagttc ttttaccatt ttagctgtaa cgtctgccat gtttaactcc tcctgtgtgt
     2341 ttttcttttt aaaaaaagct gcttcaaagg agagtgaggc ttgtcgccca gcctttgaaa
     2401 cagctccatc attcaaaatt attctgctga agaattgtcg ccttcaacaa catctacgat
     2461 ttcttcgatt gatgttgctg tgttttcttc tgtgaaatct tcttcaacta cttgatcttc
     2521 accttggttt ccttcgatga aagcatcagc catttttgaa gtaatcaatt taacggcacg
     2581 aattgcatcg tcgtttgaag ggattactac gtcgatttca tctggatcgc agtttgtatc
     2641 aaccatagct acgattggga tatttaattt gtgtgcttct tgaacagcaa tacgttcttt
     2701 acgtgggtca acgatgtaca ttacatctgg gattcttggc atatcagcga taccgcctaa
     2761 gaatttttca agacgttcac gttctttgtt caaaccagca acttcttttt taggtagaac
     2821 ttcaaaagtt ccatcttctt ccattgcatt gatttgtttc aaacggctga tacgtttttg
     2881 gatagtatcc cagtttgtca atgttccgcc taaccaacga tggttaacga agtattgtcc
     2941 tgcacgagtt gcttcttctt tgattgcttc ttgtgcttgt ttttttgtac ctacgaataa
     3001 tgctacgccg ccttcttcag caacgctctt catgtaatca taagctgcat ctactaattt
     3061 aactgttttt tgcaagtcaa tgatgtagat tccgtttctt tctgtgaaga tatatttctt
     3121 catttttgga ttccagcgac gagtttggtg accaaagtgt acgccggctt ctagtaattg
     3181 tttcatagaa attactgcca tgtttgtttc ctccgatttg gttttatttt tcctcttctt
     3241 atcttctacc ttgccggcaa actttttcaa gcacctaccg acaatccaat aagaatgtgg
     3301 atttgctgat gcataaagca ccctcagcta ttatacagac aaaagatccg tatcgcaaga
     3361 gcaaatatac ttttctgctc aacattcaat agtttacctg aaagataaaa agaattcaag
     3421 aaaaacagta gatttctctt tgtttcttta ttatcctttt tccataaaat aattttaggt
     3481 ataaaagacg aatattattg ggcaatcctt cttttttttg ttatgataca tgtgaaatct
     3541 ttcgtaaagg agaaagaaaa tgcgcaaagc tcaaagacat ttgctgatta aacaagtcat
     3601 tgaagaattc ccgatccgta cgcaagaaga actattaaac aaacttgggc attatggcgt
     3661 taccgccacg caggcaacga tttctcgaga tatccgtgat ctaaaaatcg tgaaggcacc
     3721 tgatgaaaat ggcgtttccc gcttcgtatt gtttcgcgaa aaaggaactc ctgagactag
     3781 gactgaggaa gaggatcggc tggttcaaat gattgaagac attgtattga aagtcgaacg
     3841 agtccacttt ttgacgatcg tcaatacctt gccagacaat gctcatttat ttgcatctgt
     3901 tttagatgag atcaaacctc cctctattgt cagtacaatt gctggattcg atacgatcat
     3961 ggtcatctcg gaaaatgagg aggacgcaaa acgagtagaa gaatttcttc accatccaac
     4021 tgaagtatca cttttttaat cagagcacgt attcctaata tggaatgcgt gtttttttat
     4081 tttcaatatt ttccttatta aataaacctt tacactcttt acaaaaatac atcatttttt
     4141 attcaatttt ttcaacacct tgattgaatc acaataatca tcatttatat tggatgttaa
     4201 caagagcagc ttcttgctct ttgtgtattt caaaaaagaa atgaaaggaa atcatatgta
     4261 taaattatta actcctggac cattgacgac ttctgaaaca gtgaaaaatg ctatgctgat
     4321 cgatcattgt acttgggatc aagaatacaa agaaattaca caatggataa gaaaaaaatt
     4381 attggatctt gctcaagtat cggaaggaat atacacaacg gtcttaatgc aaggtagcgg
     4441 ctcctttggg gtagaatcgg tattaagttc tgtgatcagg ccagaagata ctgtactgat
     4501 ttgttcaaac ggcgcatatg gacaacgaat gatcaacatg tgcgaatgtc tgaaactgaa
     4561 ctataccgtt tatgaggtat cggaacatca aatcctccaa gctcatgtga ttacacagct
     4621 gattgaagag aatccctcta tctccgcact agcaatggtc catagcgaga ctacttccgg
     4681 tattttgaat aatttggaag agattgctgc tgtcacacaa gaaaaagatc ttttgtttat
     4741 agtggacgcg atgtcaagct ttggtgggat accgattcgt gtagccgaac tagggattga
     4801 ttttttgatt tccagctcaa acaaatgtat tcaaggtgtt ccaggctttt cttttgtact
     4861 tgctaataaa gcggaactag aaaagcgaaa aaatgtgagt cgaagtttgt cacttgatct
     4921 atatgaccag taccgcgtga tggaaaacga gaatggcaaa tggcgtttca cttcccctac
     4981 ccatacggtt ttggcttttc aacaagcgct gaaggaacta gaggcagaag gaggaatcga
     5041 aactcgatat tcacgttatc tcgccaataa tcatgtacta cgacaaaaaa tgacagagat
     5101 ggggttccag cctttcattc aagaatatca ggggccattc atcacctctt ttctttaccc
     5161 taaggactgg cgatttgatt ttaatcattt ttatcagtat ttaaagcaaa acggatttgt
     5221 gatctatccc gggaaaatca gcgatcatga atgttttcgt atcggaaata ttggcgagat
     5281 ctatccggaa gatattttgg aactgacagc aattatcaaa gactataagg agaacgttaa
     5341 tgattaaaac tgtaattttt gattgggcag gaacaactgt ggactttggc tgtatggctc
     5401 ctgtccacgc ttttagaaat gcttttctag aaaaaggaat ccaactgact gacaaagaaa
     5461 tccgcgaacc gatgggaaaa ttaaaatggg atcatatcca acagcttctt tccctccctt
     5521 ctgtcaaaaa acaatggata gctatttatg gacaactacc tcaggaaaaa gaggtggagg
     5581 aattgtatca acaatttgaa cattatctgt ttaaagattt ggtcaagcat tctaccttga
     5641 aacccgacac attggaaacg atcgaacggt tgagaaacgc tgggatcaat attggttcca
     5701 cgactggatt tactgctcct atgatgacaa tcgtagcaga aaccgcaaaa aaagaagggt
     5761 attcaccaga cttcatggca acttctgaag acgtcgaagg gtatggtcga ccgtttcctt
     5821 atatgatcca taaaaacatg cagtacttca aaaacaagtc ggttgatgaa gtaatcaaag
     5881 tcggagatac ggtctcagat atccaggaag ggaaaaatgc cggagtgttg acagtaggtg
     5941 taattgaagg tagttcgcta atggggattt cttatgatga gtaccaaaaa ttagagatgg
     6001 ggaaaaaaga tcgtttaaaa cagtctgttg ctcttagatt ccaacaagct ggcgctgata
     6061 tttgtgtaaa caatttaaaa gagttaacag aatacatttt gtacgctgac aagaatccag
     6121 aatgactttt atacaaaatc agaggtcgtg aaaaagctgt actgcatcaa gtaataagaa
     6181 cagccaaaca aaaatagctc ctcatatttt tcgtttggtt aggcttatta ccgcagatgc
     6241 aagcttttga acaccgttta ttcgaaaaaa aaagggagct gtgacacgac ttttgtcaca
     6301 gtccccttat ttacaaacaa acagtgttta agagacaacc tctccactaa acggtgttca
     6361 ttcagctgac tctcggtatt tgctcaaaaa acgataaaat ttgaaaagct attttctctt
     6421 ttgtttctct aatactcgga tcagaacgct gatttcacat tctctttttc ttacatctta
     6481 tctggtgcat gaagtcctag caaacgaagg tcttctttca ataatacagt cactgcataa
     6541 actagagcta agcgtgcttc tttttgttca tcatcagcta aaatcttcgt atgagcatag
     6601 tatttgttga atgcttgtgc taatttgatt gcatgtttcg caatcactga cggttcgtat
     6661 ttttccccag cagacaagac cgtttcaggg tatttttgaa ctaatttaac cacttcccag
     6721 ctggtatcgt cattcaatgc atagtctgct gtttctgaag ggacaaatcc tgctttttcc
     6781 aacaggctga ctgcacgagc atgtgtatat tgcacataag gtccagtttc tccttcaaat
     6841 cgaacaactt cttccaggtt aaagtcaaat gtattcagtc gatcgttttt caaatcgtgg
     6901 aaaatgactg ccccaacccc aacttgttta gctacagcat ctttgttttc caaatcaggg
     6961 tttttctcgc tgatttgttc tttagcagat tggattgctt catttaatac ttcttctaat
     7021 aagacgatct ttcctttacg agttgataat tttttaccac cttgcgtgat caaaccaaat
     7081 ggaatatggc gcatatcgtc agaccagtca aagcccattt ccttcaagac agctttcaac
     7141 tgtttaaaat gatagctttg ttcattccct actacatata gagattgttt gaagtcgtaa
     7201 gtacgtttac ggtacaatgc agcagctaag tcacgtgtaa tgtatagtgt cgcaccgtct
     7261 gacttcttaa tcaacgctgg attcaggtca taagcagata ggtcaacgat ctctgcacct
     7321 ttatcctcgt tcagcaaatg tttttcttct aaaagtttga cgatttcatc catcttgtca
     7381 ttgtagaaag cttcaccgtt caacgaatcg aaacgtactt ccagcagatc atagatttta
     7441 ttgaattctt tcatcgattc atcacggaac cattgccata attgaatagc ctcttcatcg
     7501 ccttcttcca gacgtttgaa ccaagcacga gcttcttcat ttaattctgg ttctgtttca
     7561 gctacttcat ggaattggac atataaacga agcaattcat tgattggttc tgctttaaca
     7621 gcttcttctg taccccattt tttgtaagcg acgatcagtt ttccaaactg tgttccccag
     7681 tcacctaaat ggttgatgcg gattggctga taaccgattt tttccataat gaaaccgatc
     7741 gaattaccga taactgtcga acgcaaatgt cccattgaga ttggtttggc aatattagga
     7801 gaagacatgt cgattggtac tgttccttga ttcccgatat tgctatcccc ataatgttct
     7861 ttttctttca ctactgtttg caaaactttt ttgctgataa gttctttatt catgaaaaag
     7921 ttcaagtaag gtccgacaac ttcgattttt tcaaagttgg cagaatcgat tttttctgct
     7981 aaatctgcag cgatttgctg tggcgctttt cggtaaactt ttgctaatga aaaggctgga
     8041 aatgcaacat ctccgtgttc agctgatttt ggattttcta gtaattgttc gacttgttcc
     8101 agtgttaaat catctttgac agcgtcatag acagcttttg cgacaagatc tttattattc
     8161 atctttattc ctcctaaagt gctcccttga ttgttaaaca aaaaaaatct ctagcgacgg
     8221 catacttagt atgccatcac tagagacgag atttcccgcg gtaccactcc aattgtccta
     8281 aaaaaggacc agctccatct gatatcggta gacgaccgtc tgtttaaaac agaattctcc
     8341 aagtgcgctt cgttgtatct tcttcattcg gcttccacca cttccgaatt cgctagccaa
     8401 gaatgataca agtactctct tgttcaacaa atcttagtct tattaaactt tcttgaatta
     8461 tatcagaaaa aaaaacgaaa ggctagtcgt ttctgataaa aaaacaatcc agatggccta
     8521 aaggagaaga aataccacct ggatagctat aaatcatttc gtaaatacga aagacttaac
     8581 aaattaatca gctacgataa tcgttccttc ttcagaagct aaaagattct cgatattttc
     8641 taaagaagtg atgattgcct tagcgtttgg ttttgcttca acaaatgcaa tagctgcctc
     8701 tactttcgga agcatgcttc ctggagcaaa ttgcttttca tcaatgtatt gtttcatttc
     8761 tgaaacagta acagtttcta atttcttttg atctggtttt tggtaattga catacacatt
     8821 gtctactcct gtcaatacga tcagcaaatc cgcatcgatg atttcggcta atttttctga
     8881 agcaaaatct ttatcgatta cggcttctcg tccttctaat ccagtagctg tttctacaac
     8941 aggaatccca ccgccaccaa cagagactgt gataactcct tgatcgacca gcgtttcgat
     9001 gacacgtgct tctttaatcg agatgggttt aggggaagcg actacttttc tccatccacg
     9061 tccagcatct tctttgaatg tcacagtgga gtcagcattc atttgttctt tggcttcttc
     9121 ttccgtgtag aatggaccga ctggtttact tggatttttg aatgaaggat cattttcatc
     9181 cacaataact tgagtgacta aagaaaccac atctttatcg atcccttttt ccttcagtac
     9241 ttcgcccatg gcattttgta accagtaacc gatagatcct tctgtcattg caacacatgt
     9301 atctaaaggc attgccggtg ttttctcaga atcagcagct tgctgctgaa tcaataggtt
     9361 accgacttgc ggaccatttc cgtgggagat gatcaattca tctccttgtt cgataaattt
     9421 cactaaatac tttgctgttt ccattaacgc ttcttgttgg gcttttgcac ttgcatccgt
     9481 agacaagatt gcattacccc ctaaagcgac aactactttt cgtttggcca tttcattcgt
     9541 cctttcttca gttctaccga attttctaga taacaaaacg agccagcgtt ttgtgatttt
     9601 ccctcaatgc tgactcgttt tttcgtgaga tgaatgtggt tatgcttaaa cgcgagggat
     9661 aaacaagtta cctaatgttg ctgccatgat cgctttgatt gagtgcatac gattttctgc
     9721 ttggtcaaat tgacgagcat atttgctgcg gaagacttca tctgttactt ccatttcagt
     9781 gatgccaaag cgttctttca tttgttcacc gtaaacagtg tttgtatcat ggaaagctgg
     9841 caagcagtgc aagaagatca aacgatctgt gttgtgtgtt ttttctacca tttccatatt
     9901 gatttggtaa ggtttcaaca atttgatacg ttcttcgaat ttatcttctt cacccattga
     9961 tacccataca tcagaataaa ggacgtctgc tccgtctaca cctttatcaa catcgtcggt
    10021 aatcatcaat tgtgcaccgg atttttcagc aaagccttct gccattttga cgatttcttc
    10081 ttcaggttgt aattcttttg gtgcaacgat acgcatattc gctcctaaaa ttgcacctgt
    10141 gactaataaa gagttagcca tattgtttcg accatctccg cagtaagcaa cagtgatacc
    10201 ttctactgtt ccaaagtttt cttggatagt taaaaagtca gcgatcattt gtgttggatg
    10261 ccattcatca gtcaagccat tccaaactgg aacaccagag aattctgcta gttcctctac
    10321 cattttttga ctgaatccac ggaattcgat tccatcaaac atacgaccta gtactttagc
    10381 tgtatcttct gtggattctt ttttacctaa ttggatatca tttgcaccta gatattcagg
    10441 atgtgcacct agatcgatcg ctgcagtagt aaatgcagaa cgagtacgtg tagatgtttt
    10501 ttcaaataaa agcgcaatat tttttccttc tagatagtga tgtgggatac cacgtttttt
    10561 caagtctttc aagtgttctg aaaaatcgat caaatattgt aattcttctt ttgtaaagtc
    10621 tttttctgct aataagcttc ttccttggaa tactgattct ttcatattaa acattctcct
    10681 ttagattgtt tttatttaag attaaagagg aaatcccgac ggcagctgac agtaagaccg
    10741 ccgtcaggaa acaaattact tcaagtcttc acggacaagt ggttggctca tgcagcgtgg
    10801 accaccacgg cctcgtgaca attcacttga attgatttct aacactttga tgccatagct
    10861 acgaagcaat tcgttcgata cataatttcg gttataagtg actacaacac ctggagcgat
    10921 tgccaatgtg tttgatccat cattccattg ttctcgaggt gcaacgatct catctccatt
    10981 accagttggg atcagtacaa gatcatctaa tccaagagca gctttcatcg tttcgtgtaa
    11041 gttatctgaa tgagtaatct tgatatcatc accatctggt tcgattgtaa agacatccat
    11101 cttaccgtct ttactttgaa ttgctggatg gatagtgaat ttatcgtgat ctaccattgt
    11161 aaatacggta tccaaatgca tcattgcacg gttgtttgga attttgattg ctaacacttt
    11221 ttcaaatcct gaattttttg cgaataggtg gcgtgctaat ttttctaaag ctttcgcatt
    11281 agttcgttga gaaatcccaa cagctactac tttatcgctt aagatcaatt cgtctccgcc
    11341 ttcgatatga tctggatttt cacgatccaa ccatacttcg attcctttat ttgcaaatct
    11401 tggatgatgt ttcaaaatat attctgtaaa catcgattca cgacgacgtg cttcaaaagt
    11461 catcttattg actgtcatcc catttcccat tgatgcggaa gggtcgcgtg taaagtacaa
    11521 gtttggcatt gggtccatgt aaaatggata gtcatcgtct gctgacagat cgtatagact
    11581 gctagaacgt acttcaatat cttttgtacg aaccccagcc atgattttat ctaccatttc
    11641 ttgcgtttcc attgatagaa ggaactcatg aagaccctct cttacagcat tcgatgcaat
    11701 atgagattca tccaacattt tattcaagaa ttgttctttg acattgcctg cgtcaatcgc
    11761 ttctgccgct aatttttcca agtaaagagt ctcgacgcct tcattttgca atgtctttgc
    11821 aaagttgtca tgttcttctt gagcaatcgg aagatatgga atatcatcga acaacagacg
    11881 atccataata tctggtgtca aattttccac ttcttgtcca ggccttttca gtaaaactgt
    11941 ctttaatttc cctatttcag agaaaacgtg aataggttta tccatgttgt gttgcctcct
    12001 tctttttgat tacatgtata ggatatcctc cttttgtaat cactttcata attttgtctc
    12061 attcaaaatg
//
LOCUS       DAIGOK010000086        11772 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.086, whole genome shotgun sequence.
ACCESSION   DAIGOK010000086 DAIGOK010000000
VERSION     DAIGOK010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.086"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(<1..164)
                     /locus_tag="NOA01_001921"
     CDS             complement(<1..164)
                     /locus_tag="NOA01_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633105.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMP"
     gene            complement(180..794)
                     /locus_tag="NOA01_001922"
     CDS             complement(180..794)
                     /locus_tag="NOA01_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCI0633106.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(941..1624)
                     /locus_tag="NOA01_001923"
     CDS             complement(941..1624)
                     /locus_tag="NOA01_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCI0633107.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(1733..2191)
                     /locus_tag="NOA01_001924"
     CDS             complement(1733..2191)
                     /locus_tag="NOA01_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCI0633108.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(2275..4038)
                     /locus_tag="NOA01_001925"
     CDS             complement(2275..4038)
                     /locus_tag="NOA01_001925"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCI0633109.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(4259..5719)
                     /locus_tag="NOA01_001926"
     CDS             complement(4259..5719)
                     /locus_tag="NOA01_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633110.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIVLSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(5927..6853)
                     /gene="glsA"
                     /locus_tag="NOA01_001927"
     CDS             complement(5927..6853)
                     /gene="glsA"
                     /locus_tag="NOA01_001927"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCI0633111.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(6876..8303)
                     /locus_tag="NOA01_001928"
     CDS             complement(6876..8303)
                     /locus_tag="NOA01_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCI0633112.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(8334..9548)
                     /locus_tag="NOA01_001929"
     CDS             complement(8334..9548)
                     /locus_tag="NOA01_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HCI0633113.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(9889..10131)
                     /locus_tag="NOA01_001930"
     CDS             complement(9889..10131)
                     /locus_tag="NOA01_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCI0633114.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(10163..10720)
                     /locus_tag="NOA01_001931"
     CDS             complement(10163..10720)
                     /locus_tag="NOA01_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCI0633115.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(10733..10921)
                     /locus_tag="NOA01_001932"
     CDS             complement(10733..10921)
                     /locus_tag="NOA01_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCI0633116.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(10934..11500)
                     /gene="amaP"
                     /locus_tag="NOA01_001933"
     CDS             complement(10934..11500)
                     /gene="amaP"
                     /locus_tag="NOA01_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCI0633117.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         3452 a         2441 c         1928 g         3951 t
ORIGIN      
        1 ggcatttcta tttccttacc atctaaagta acagtagtaa cgacaaaatg gtcttgcaat
       61 ggcggaactt ctaagtactg cccttgcaga aaatcattca ctgtttcata ttcttttttc
      121 atttcttttg aatctgtcga tggtttatag cttattttta acataactag tgctcctttt
      181 tattcgggac gtgcaatcgt gaaaatttct cctattgctg cataatcatt tcctgccaaa
      241 cggcttacag cattcaattt tcttggatcg atccttcctg tcttatcatc atagactgtt
      301 tcatttaaat gatatttctg tacttctagc aaaaataaat cagctgttac tttattttta
      361 ttccgtatct cgacatgttc atataaggat gtttcaaaac gaacttttgc ttctgtcaat
      421 gctggtactg aaatgattac tgaatcagtt aaagtcaagt cagtcaggta cagttcgctt
      481 tcctcattag gcagattggc tgctgtttta tttacttctt ctacgttgtc ctcatcaagg
      541 atatggataa cagcttctct tgtctcaaga atattctttg ctgtgtcttt tactgcatct
      601 tgttttctct ggatcgccaa ggataagatg ggtcgattcg aactgacgat attgaagtaa
      661 ctgaagggtg cgatattcac tatatctttt gctgatttag tcgttactac tgctactggt
      721 ctagggatga ctgaaccaat cagcaacttg taattttcac gttctgtcaa ttctgaagga
      781 ctccgttcaa acatggtttc gccttctttc tataaaattc gttaaaaagc agatcttgaa
      841 gacaaaatga aaagtaatgc ttttttcatc ttcttagaat ctgctttaaa ctatctatca
      901 atttttcttt tttgtcaaga atctgtgccg tgcgtgtttt ttagttattt gctttacttg
      961 cggcttcgac atatctttta gtgatcaatt gcggacgggt gataatagat ccaactacga
     1021 ctgaaaaagc acctaattca tatgctttct ctacttgttc aggagagtca gtatgtcctt
     1081 ccataattac tgggatatcg acttgttcta tgatttgatt caataaatca aaatcaggtg
     1141 tctgcgtctt tcgtgaatat tctgtatagc ctgctaaagt cgtagacaaa agatcaaaac
     1201 catcttcttg cgcttgaagc gcttcctcaa aagtcgaaat atctgccata gctaaccgac
     1261 cgtgttcgtg aatcatttgg acaagttcag cagttgtttt ttctgtcgga cgtttacgag
     1321 aagttgcatc cagtgcaatg atttctgctt gcgtctccaa aagttcttcc acttcttttt
     1381 ctgtcgcagt aataaacact tctgacccat cgtaatctct tttgataatc cctattaccg
     1441 gtaaatcaac gattcgcttg atttcattga tatcttctgt cgtatttgct cgaataccaa
     1501 ctgctccgcc ttcttttgca gccaaagcca ttctccccat aataaaagaa ctgtgtagcg
     1561 gctcgttttc caaagcttga caagaaacga cgactccgcc tttgatttgt tccagtgtat
     1621 ccacaatatc tcctccagta atgttatata aaaatacagc ttcatataaa aatgtaaccc
     1681 ttttctattc aggagacaat aatgagctat ttctttcagc agacctttct tactaaaaga
     1741 aaattacttc aaattcttcg tatacgacca gcttctcttc tgtaaattct atgttcaacg
     1801 cttctttata agcatgttta aaaaactcag catggacttt ccgccaataa gctaatgtcc
     1861 gatccccttc tccttctttc caagcatgct ctttcgtgac ttcactaaaa ggcaagatgg
     1921 tcacatttat atttttgatg atacaaactg gttcattttt tccattcagg accacgtcta
     1981 attgaccaac ttttggtaaa ggctcattct cttgtttata cagatcgtaa ccacttgatg
     2041 taccagtctt tatctttttt acaactagtt cgctcaactc gtctgccatt ttttctgtat
     2101 ccccaaatga aaagaaacct aatttttctg attgataact aggatatttt tcttggaact
     2161 ttttgacata ttcttctact tgctgataca taacatccac ccctttgctt tttataatga
     2221 aaaaaccatc cagatactag tataaacaat tatctggatg gtgagcataa tgaattattg
     2281 aatattcaag acacgcagac tatcttcaac gatttgtttt ggtgttaaat gataacgttc
     2341 ataaagctgc cctacaggaa cgctatccgt gaattctttc tctgcaccaa aatttaatac
     2401 gttcattgaa gaatcaccat aataacgact gatattttcg ccaaatcctc ctgacaaact
     2461 accatcttct aatgtgatga ctaattcgtg tgtttctttt aatttatcta atgtttctac
     2521 gtcattttct gtgatcgttc ctggattgat caacgttgct tcgattcctt tttttctaaa
     2581 agcttttact gtttcttttc ctaattcaag aaaaccgcct aaagctaaaa ttgctacttt
     2641 tcctccatta tttactgttt gataggctgg aatactataa tcatttgcga tagctgtacc
     2701 atgttctaca ccatgtgtag gaattcggat tgctacaggc tgcgctgttt gttcgaacgc
     2761 ccaatctagc atagagatca tatcctcttt tgttgctgga gcaagatatt tgatgttcgg
     2821 gatacttgat aagtaaggaa tgtcaaatgt tccttggtgt gttcggtcac tagctgagat
     2881 cgttccgcca ccgactaaaa taatcactgg attctcgttg atcgctaaat catgtgataa
     2941 ttgatcatat gcccgttgca agaaagtact tgattggaag acgactggac gtactccctg
     3001 agaagccaaa gcagaagcga aagtgataga gtgctgttca gcgatcccca cgtcaaaata
     3061 atgatctgga tgtttttctt ggaatgtttt cagtccaaag gcgcctggta tcgctgcatt
     3121 gactgcggca ataggagctg tacttgtttg cattttctta tccaaataat ccatgatgat
     3181 catattaaat gtttctccag aagttacttg ctttgcctcc ccggtggaca gatcaaatgg
     3241 tacatgccaa tgatatttca ttttattatc gattgccggc tgataaccgc gacctttttc
     3301 cgtatggacg tgaagtacaa tcggatgatc aatatctttt acttcttcaa ataaacggat
     3361 caccgtttcc agatcgtttc cattatccac gtaacgataa tcaaatccca ttgctttaaa
     3421 taggttattg ctgcttttac cgttggtttg acgaagctca gctaagtttt tatataaact
     3481 gccatgattc tcatcaatag acatctcatt atcgtttact aatacgatta gattggattg
     3541 taaaacacca ccattgttca atccttcaaa agctaagcca ccacttaggg agccgtcacc
     3601 gataaatgct accacatttc ctgattcttt cttcagatct ctcgctttag ctaaacctgt
     3661 tgctaagctg atagatgtag atgtgtgccc cactgtaaaa tagtcatgtt tactttcatg
     3721 ttggttggaa tatccgctca cttcacggta atgtcctttt tcaaaaccat tttttcgacc
     3781 agttaaaagc ttatgaccat aagactgatg ggaaacatcc caaacgattt tatcctttgg
     3841 tgaatcaaat acataatgaa acgcaatcgt taattctact acacctagat tcggaccaac
     3901 gtgcccacct acatcgctgt ccttttgcaa gattttttgg cgcatctctt ctgccaactg
     3961 tttcatttcc tcaacagata attttttgac atctgcgggg ctattgattg catccaatac
     4021 atgtaatact gtactcataa tacattctct cctccgtact atcttctttt aatgaaaata
     4081 gatttgttac actttatcta ttctactact tagagttgac tctaagtcaa tagcatttta
     4141 aaaaaatttc agagaaagct tgttatataa gtagatcttc gcacgaatca ggactcaaat
     4201 aaaaaccttc tacagacaat taaaaagctt tgtagaaggt ctcctaatta aggtctcatc
     4261 aagaaatatg tttttttaat aggtgaagtt cctgagcaat atgatacaaa tggatgtttg
     4321 caccttttaa tgagattgga tagatctttg tcttttcagt ttctaatcca ttttgatagc
     4381 tagcagcatc gcacaaaacc actctttttt tcgcacatag acattcatca ggcaattctt
     4441 ctaatgaacg aaataaaata ggatttattt cagcgatgcc ttcttccgca tacaaatgaa
     4501 attgggttcg ataaaaaaat tgtttgactg aactttctga aacaacaaaa atttctattt
     4561 cctgtctccc cgcttgttca ctgttgatca aagaaagact tttgacgaat tttcgaatga
     4621 aattttcatt ccatcttagt tggatttgat ttttcttttt ccactcttcc aatatttcta
     4681 caatttttcc gtacagaagt tgttcttttt ctgatagaag atagatcctt tccggtaaaa
     4741 atacttgtcc atctgcccac aacgtacgga caaattgtaa aaaggattgc ttgaaaataa
     4801 aattttctgc atcgtcgatt tcagcgatac taataatctg taaaatcaat tcattcagtg
     4861 ataggtcatt gagaacaaaa tttttataaa caacttcgaa atcttttctt aacagctcca
     4921 tgttctcatt ggtataattt ctactgttga acaaagaaaa aatataatag acatcttctt
     4981 cttgtaagtc tagaattcct ttttgctctt ccaatccttg acagactaat tgaaataacg
     5041 gcttcgtttc tgcgcttttc ttatcttctt ctttcagtcc aaggggaaat gcctttcttc
     5101 cgattgcgat ctctattcct cgtatgataa aggacttttc atcttccgaa tatcttcttt
     5161 taaaaaaatg agtctctaca taatcaatca actcgttaca agcttcttct acatacggat
     5221 ttttattttc atagccttcc caattgacat aacgtactaa agctagcaag atgttccgcg
     5281 agtcaaattc tgggataatg atttctttat ttttcaaata atcattttct ttaaaaaaat
     5341 ccaatacgat ttttttgatt gtatatactt tgctcaaaga aataaattca agatcagcca
     5401 gttctgtact cgtgaatata ccattaaaat aataacttaa aaactttaaa aaataagatt
     5461 gaccgtacag aaactgtgtg agctcagaaa gaacaatacc atgtttgaat acgagtttat
     5521 acttatgctt ctctcttact tgttctttta tacaatattc tagacactcg gctagacttt
     5581 ctagatcgtg aaggatcgtt ggtgtagtca cccttagaga aatagctgtt tgttcgatgt
     5641 ctatttcttg gaattcaaat aattgttcac acagatacac ttttctaaat atatttcgct
     5701 cgatgtaatt ttccagcatt cctgatacac gcctccagtt tttcttatga ttttatttaa
     5761 tcatatctgt aaatcgtatt tttgtttcct ttttatttag aaaagccctt tcaaaaacca
     5821 ataaaaaaag aaactgggac aaaagtcaaa caagctcaca atttcagcct cattgtaggt
     5881 agctttgagc cgtttggact tttaccccag cctctttcaa gaatggttat tcaaagatgt
     5941 cagcgtctag ttctttgact acatctttca acaattggat accagctgcg ctgttaccaa
     6001 atggatctag tgccgggctg aagacaccta tgccgtaacg atttggcaca gctgccatca
     6061 acccgccgcc tacaccgctt tttgcaggta caccaacatg gacggaaaac tctcctgatt
     6121 catcatacaa accagcagtt gtcataattg atttgacgat tgtagcactt tcttcagtaa
     6181 tgatttgttt gccattccaa ggagcaattc ctttatttgc cagcacagca gctaattttg
     6241 ctatgcctgt agcggtgaca ttgagggagc attgtctgaa ataagcatcg agtatctccg
     6301 gtacgtctcc ttcgatcatt tgattacctt tcatatagta agcaagagag cgattgatat
     6361 cacctgttct tgattcagaa tgatagattt cttcattcaa tgtgatatta ggatcatcac
     6421 agatctcttt cataaatgac aagattcgct caaatttttc atctgcatct ttaccggcaa
     6481 tcaacgaggt tgttgcaatc gctcctgcat taacaaaagg attcattgga tgtttgcgat
     6541 gattgatttc catattcatg atagaattga aggcaaaacc ggttggttcc gtgttgattg
     6601 tttgaaagac tttttctatt ccgttgtctt tgattgctaa taacaatacg ggaacttttg
     6661 acatgctttc gatggcaaag cgcacatcgg cgtctcctcc ctcaatcagt tgttttgtat
     6721 ccaaatcata aatcgccata cctaattgtt tgggatccac ttcagctaat gctggaatgt
     6781 aatccgccac ttgtcctgtt tgataatagt ggaaattttt tccaatcaat tgttctaatt
     6841 cttttacttc catttgaata accagctttc attttttatt tttctgcatg ctggaattta
     6901 tgacggcatg cgtagatcgt aaatggaata actaagacga taagataaga aacaatcaga
     6961 atcgtttcat aaatcccatg ctgtccagct gcgatactag caggtggaac aaatgagata
     7021 caaaaagcaa aaagtgaagt caaaagacct aaacaagcaa cgattacttt tacaactttt
     7081 ccgcctggaa cctgataggc tctttcaaga ctgtcatctt taaaaaccag cttcaaatag
     7141 cccgcgaaaa acataaagta caacatcagg tagattacaa cagtcaatga catcgctgca
     7201 agaaatgaaa gattattccc cccgccgctt aatgtaaaga cggctgccca gattgttgca
     7261 acgatcccat ttgcgatcat caaatgagca ggaacaccgt gtgtgttttc ttttgcaaaa
     7321 tattcaggaa gtaatccttc ttctgctgct acgtgaagtg cttttgaagg agaggttacc
     7381 caagaagcaa tttctccgat gacacctaaa caaacaagaa tcgcaagaat tttcgcaatc
     7441 catgcaagat ttggatcaat gtgtcttaat aggaattcta acgattgaat cacccctgta
     7501 ttaagagaca aaccatgttg aggtactgta gtagcgacgg tcaatccgcc aaaagtatct
     7561 aaaatgattg ccagaataac caatacaaac attgctaatg gataatcctt tttagggttt
     7621 tccatttcat ttgcatgact ggcagatgtt tctacgccca tatatgctaa aataaaggaa
     7681 acaaaaacaa ccagcgtagg caatttcgaa aaatcaggaa tcaatgtttt catagacagt
     7741 atcgtttgta gtggagcgcc tgaagcgaca taatggatcc ctaagccaaa caagactaat
     7801 gctggcaaca agataccagc tacaaaaccg atccgagcaa tcaatgaagt gtatttggtt
     7861 ccttttaact gtgagagtaa aatagcccag aaaataatca ataggattgc caatttcaga
     7921 tatttattgt tttgtatagc accaataccc gttgcatagg ataatgcacc agttaagaaa
     7981 tacagcatgg tcaagtagcc aactgttatc tcaaaccatt ggaaaaagat tgcggcaaaa
     8041 ccgaatcgtt ttcccaatgt gcgactgacc caagtataga caccgccttt ttcccatcct
     8101 ttcaccgttg ccatctctgc tgcgcacaat gccacaggga taaaccatag caaaccgcct
     8161 aataacaaga aaaatactaa gctgaagcct gaggtcgcga atgtgggata ttcatataat
     8221 gaaacgacca ttgagatggt catagccaaa aagccaaaga acgttaattt ttttgcagga
     8281 attttcgttt tgctttgttg catgaatctt cctcttttct atttgaattt gtttcaattg
     8341 agtttgttgc caaagaagaa aaagtccaat ccttcttctt gttcttcttt tgtagtagtg
     8401 atcgttgtaa agtgcatcgc cgattttaaa atcactgttg tcagcacata ggcatacacg
     8461 gcagtaatga tgacaccaat cacttgaatc cctaagaagt accagctgct tggattcgtt
     8521 agtaaatggc tgttagatgc aaagatccct atcagaatcg tacctgtgaa tccgcccatg
     8581 ccatgaacac cccacacatc aagtgcatca tcccattgtt tctttttacg aaaatcaaca
     8641 gcagcatgac agacgatacc cgcaatgatc ccaatcagca tggcactttt tgcttctaca
     8701 taaccggcac aaggcgtaat ggctgccaat ccagcaacgg atcctgttag tacgtcgaca
     8761 aaatcaaagc catttccttt tacttttgca atgatcagcc aagtaatcat cgcaaatgca
     8821 agagcgatga atgttgaacc aaatgcattg actgcttgtt catctgctcg aagtgcacca
     8881 ccggaattaa aaccgaacca gccaaaccaa agcagtcctg taccgatagc agctgccata
     8941 agactgctat gctgacttct ttctcctaaa ggaatctttc tttgaccaag tgtgaggata
     9001 catgctaaag agccgaatcc agctgtcgtg tggatgactg ctccaccagc gaaatctcta
     9061 aatcccatct gatccaaaaa tccgccaccc catatccagt ggcaaacagg gaaataaatc
     9121 agcagattcc aaaaaataac aagtataata tagcctttta aattcaatct ccctgcaaaa
     9181 gctcctgtca tcaaaggaac ggtaatcacg ataaacatca gttggtaaag aaagaacatt
     9241 aaaaatggaa tcgttgcacc atgaaacatg ttggggaagc ttgtaacatg tctcatcaaa
     9301 aagaaatctg ccgggtttcc aataacaccg ccgatatccc gtccaaaggc caatccgaat
     9361 ccaccaaata cccacataat cgttgttatc ccaacggaga gaaaagattg agacatgatc
     9421 gttagtatat tattcttccg tgctaatccg ccgtaaaaaa aagctagacc aggagtcatc
     9481 aggcaaacca ttgctgtaca taaaatcatg aatgctgtat ctcctgtatt gataggtaat
     9541 gtattcattt ttttcgcctc ctttctacga ttacgagtat aaaagaaggt gtagttgttt
     9601 ttttcttctg tttttttgaa ttaatacttg catttataaa agacagacaa ttaataaaag
     9661 cttgatgtgt tgtgctcttt ttcgtttttc acacaaataa agacaaaaaa taaacggtct
     9721 tctttttata gaaaaccgtt tattttcgtc tttttttatt tttaaagata ttttttcata
     9781 aggaataaag aatacttcat ataaaatata taaaaaagaa ccgcagcgag tgtcgctacg
     9841 gttctcaaat ttgttcttca tttcaacata agtttttctt caatttgctt ataatttacg
     9901 tgcaaagaat gaaacgacta atactaaaat gattgcaccg atcaatgatg gaatgatagc
     9961 cattcctgca attgttggtc cccaagttcc tagtaaagct tgtccaattg ctgaacctac
    10021 taaaccagca atgatgtttg cgatgatccc cattgatgat cctttattcg tgattgctcc
    10081 tgcaattgct ccaatgatac cgccgacgat taatgcccaa ataaatccca ttttaaattc
    10141 ctcctaactg acttcttact tcttattgta cacgtggttc gttattttct ttgacttttt
    10201 gtgcaccttt gcctacagca gattttgctt tatctgtttg gcgtgaagca aattgtcctg
    10261 tggattttgc tgcttctgtt actttgtctt gtactgtttc ttgatctttt tggtattctt
    10321 cttgtgtttt gatgtctgca acattcacat tcacttcgat aacatctaaa tgtgtcatat
    10381 tgtttacttc agtactgatc aattctttga tttggttgta aatatcttcg atgtctttac
    10441 cgtattctac gatcacatct agatccacag ctacttgttt cttacctact tctgtattga
    10501 tacctgctgt tacgttatct gtgttgacta atttttcagc catatttgag aaaaatccgc
    10561 catctaatgt caatagacca tctacatctt tcaatgcata accgatgatt ttttggatga
    10621 ctttgtcttc ataagtcaat tcaccattca tcatgcggtt ttctcctttt tcatttgcca
    10681 aaactgatcc atttgttgta ttcatgtttt tgttttccat ctttgtttcc tcctatgatt
    10741 taaaaaattg atcaactatt cctgactgtt tcaaataaaa accgattcct gcgccaattg
    10801 ctgtcatgat tatgatcaac aatgtcttga aaaatccaat cgttaaccat aaaacagcta
    10861 gcagtagtcc aagaccccca aagataatag ggagcttgta tgctgaaaat agttctttca
    10921 ttatttggcc ctcctattcc actcgagagt gcttgtagtc tcttgtatct tcttgttctt
    10981 cataaccgct gtatgtgact gccactttga ccgtttcttt tgttccgagt actttagtta
    11041 cttcttcttc gatatcttgc atcaatacac ctgttttccc tactaatgaa gaagttcgtt
    11101 tcaagtctcc gttgacatgg atatggattc gatttttcgt tgaacgtatc cgtactttag
    11161 gtgaatgaat gaactcttct tcgtgtaaat gggaacgtac caacccttcg atcgcttttt
    11221 tgtcgattgt tagtttgcca tcttcccgtt tcataacgaa agttcctctt gactttggat
    11281 agaaaagaac gactagcaag atgataatag caagaacggc aaatactaca gctgcccaga
    11341 aaatatattg ttgcatgtag taattcgtta aagtaaagaa gcgaaatcgt tccaattgtt
    11401 caggcataat atacggcgca ttgatcaacg caatcgctag aaatactgaa agtagcagta
    11461 aactcatgat aacaccgatt atttttaacc ccttattcat tttccacctc ctataaactt
    11521 atttttaccc tgtaaataac tctaccgaca atcacagtat agatagcgaa cagttagcat
    11581 agcatcagta aataaagcgc taccaaacat tgctggatca taacggcgtt ttattgccag
    11641 tctgatactg aaataacaat tgcttactta ttctctattt acttttgatt gctggctata
    11701 cttaaaggat attattcaag attaaaaatt gcaaagatta tgctcagtta ttattacaag
    11761 attctattta tt
//
LOCUS       DAIGOK010000087        11715 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.087, whole genome shotgun sequence.
ACCESSION   DAIGOK010000087 DAIGOK010000000
VERSION     DAIGOK010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11715
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.087"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(<1..819)
                     /locus_tag="NOA01_001934"
     CDS             complement(<1..819)
                     /locus_tag="NOA01_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCI0633118.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKN"
     gene            complement(792..1472)
                     /locus_tag="NOA01_001935"
     CDS             complement(792..1472)
                     /locus_tag="NOA01_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCI0633119.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1495..2433)
                     /locus_tag="NOA01_001936"
     CDS             complement(1495..2433)
                     /locus_tag="NOA01_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HCI0633120.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2678..3541)
                     /locus_tag="NOA01_001937"
     CDS             complement(2678..3541)
                     /locus_tag="NOA01_001937"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCI0633121.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(3683..6256)
                     /gene="mprF"
                     /locus_tag="NOA01_001938"
     CDS             complement(3683..6256)
                     /gene="mprF"
                     /locus_tag="NOA01_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCI0633122.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFLSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6397..7203
                     /gene="proC"
                     /locus_tag="NOA01_001939"
     CDS             6397..7203
                     /gene="proC"
                     /locus_tag="NOA01_001939"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCI0633123.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7282..8838)
                     /gene="rny"
                     /locus_tag="NOA01_001940"
     CDS             complement(7282..8838)
                     /gene="rny"
                     /locus_tag="NOA01_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HCI0633124.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9249..10298)
                     /gene="recA"
                     /locus_tag="NOA01_001941"
     CDS             complement(9249..10298)
                     /gene="recA"
                     /locus_tag="NOA01_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCI0633125.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10407..11648)
                     /locus_tag="NOA01_001942"
     CDS             complement(10407..11648)
                     /locus_tag="NOA01_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HCI0633126.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
BASE COUNT         3368 a         2606 c         2034 g         3707 t
ORIGIN      
        1 attttttctt ctgaaggcaa ccaatattcg tttgatgata tttacaatca ggaacaaagc
       61 aataaagact aatgtgattg ttgctcctgg aggtgtatct agataaaacg agctagttag
      121 tcctgacagc atccctagaa atcctacaca aacactgata gcaatcacga cattgaagct
      181 tttgcctaat cgcataccga ttgctgcggg aagaaccatg attgcggata tcaaaagtgc
      241 accggcaatc gggatcatca cagcaatagc cacaccagta atcacattga acatcatcga
      301 catcaaatga acgggtagcc catccacgtg ggcggtatct tcatcaaaag tcagaacata
      361 catcggccgt ttgaataaaa agaacaatat accgatggca acgaataaga ttcctaaaaa
      421 gatgacttgc tcccatgtaa tggtcacgat cgaaccaaac agataggatt ggatacttgt
      481 tgctgaattc cctccgctca aattcatcaa tactaacgct agcgccaaac caccagccat
      541 taaaatggca atggagattt ctgaataggt tcggtagatc gttcgcagat actccaatat
      601 cacagctgct aatacaacga ctagcatggt cgtaagtgta ggattaaggt tcaaaaagaa
      661 tcctaacgct acccctgcta aagagacatg agaaagcgta tcagccatca gcgattgtct
      721 tctgatcact aaaaacacac ctagcatcgg ggcgattccg gcaatgaaga tagcggcaag
      781 aaacgctctt ctcatgaatt catatgaaag caacgccatt gagaatcctc ctttctcacc
      841 agtcggatct gtctgtccgc atactgtttg atatcttcat gatcatgggt gatcattaga
      901 actgatttcc catggacatg cgcactatgt cggagaagac gataaaattc atttctggat
      961 tcttcatcca tacctgttgt tggttcatca agaacaaata agtcagggtc agtagcaaaa
     1021 atacgcgcta aactgattcg ctgcttttgt ccgccagaca attcacctat tcgccgattt
     1081 cgcatatccc acatccctac agcttctaaa gcacgctgta catgttgatg atctttttca
     1141 gagagacgct tgaaccagcg gcctcttgga tatcttcctg aacggactaa ttcgatcacc
     1201 gtactaggaa aacctgcatt gaaagatgca acttgttgag gaatataacc gatacttaat
     1261 ttttttcctt cttgattttt ctcagcaatc tttatcattc cttttgaagg tttcagtaat
     1321 cctaacgatg ctttcaccaa ggttgacttg gctgctccat tttctccagt taaaatgaca
     1381 aattcacccg catctacatg ataagagaca tcttctagta caggttcatc atcatagtaa
     1441 aaggtcaaat cagatatttc gatataacgc accttacaac ctccattcta aaacttaatt
     1501 gatactttct tttaaagctt tcagattttt ttccatgaca gaaatataat tttcgcctgc
     1561 tttgatttgt tcattagtca agctttctaa aggattcaac acttctagct tcactcctgt
     1621 ttcattcgac aatgtctcag ctaccttcga agaggcattt tcttcaaaat aaatcacttt
     1681 gatgttgttt ttcttcacaa attcttttag ctcagctaag cgacttggtg tgggttcctg
     1741 atctggtgaa acgcccgaaa tcgctacttg ttttaagtcg tattgattcg ctaaataagc
     1801 aaaagcagca tgctgagtga caaatgtgcg gtttgttgca tcttttagct catcggagta
     1861 tttttgatcc agtgcttcaa gctttttgat ataagcatcg gcattcttcg taaaagtttc
     1921 ctttttatct ggatattttt cgcctaattc ttcagcaatc gttctgactt cttttatagc
     1981 taataccgga tcgagccaaa catgtggatc gtattcatgt gaatgaccat gcccttcttc
     2041 tgattcttca tgttcgtctg cttcttgcga ttctaaacgg tcgattcctt tagctgcttc
     2101 gatcatcaag gtctgtttgc tatccactga atcctttaac gaatccacaa aaaattccat
     2161 gtctgaacta ttatacacga aaacatctgc atcggaaatt tctgctcttt cttttgcact
     2221 tggttcgaaa tcatgcggct ccgttccagc agggatcaaa agtttcacgt ttccttcatc
     2281 ccctacgatc tcttttgtaa aatcatacat cggataaaaa gtcgtaacga tcgtcaaatc
     2341 ctcttttttg tctgcttcct tgttcgtatt tccacatgct gcaaacagta aagcacctat
     2401 cattaccgta gctgtcaaaa gatatttttt catttatatg ctcctttcaa tgaacaaatc
     2461 ggaattttac gatttattaa cgtgaactaa tgtatcaaaa taaaaaaaca cagtcaatag
     2521 aaatccatca atttctgatt taagcatgta tattcttgat taaatcgtaa aatggatgag
     2581 ttgctgctca tatctagcca tttcactaca ataaaaaact gaagaatacc attttaggat
     2641 tcctcagttt ttcatcacct attttacttc ttatatttta ttgcagggtt cgtactaaat
     2701 aaacttcctc acaaaaacct tttaacccat tatatactct ctgcgctctt gaataatgtt
     2761 gacacaaggc aaaaacggtt ggtccgcttc cactcatcaa tgcagcatct gctccgtact
     2821 tgatcatacg ctctttgatt tgatggatga ctggatgccg ctttcccgtt atatcttcta
     2881 aactgtttcc catttttttg atcatctgct gataatcttg ctctcggatg gcttcagcaa
     2941 gtccgtggat gtcttgatgt tggatggctt gcgtatctac ttctctaaac actgtacgtg
     3001 tagagacact cattcttggt ttgaccaaca caacccagca ctgtggcata gaaggtaata
     3061 cttggatttc ttcccctttg ccaccgacaa atgccgtact tccgtagaca cagtaaggaa
     3121 catctgtacc gacttccaaa ccgatttctg ccagttcttc taacgaacag cccagattcc
     3181 ataattggtt gatccctctt agagcagctg cacagtctgt acttcctccg cctaatccag
     3241 ctgctactgg aatattcttc ttaacaaaga tcttgacacc ggttttgata ttgtatttat
     3301 tttttagcaa taaagcagct tgataaacat tgttccgttc atctaccggt aaaaatgctt
     3361 tatttgtttc tacaatgatt ttgtcttctg ttatttcttc aaaaaataga tgatctgcca
     3421 agtctacgct cgacatcacc atttccaact catgataccc atcttgccgt ctatataata
     3481 catccagtcc taaatttatc ttggctggcg ctttttcaat caattccatc ctctcaccca
     3541 ttcgaaaagt ctaagtcgca agtattctat catagggaaa aataaaagta tagcttatgt
     3601 ctgcctagtg gttgattttg tcatcgtttc aacagctttc acagaatttt ctcttcataa
     3661 tccaaacaag ttctagtgat tcttataatt ctgtgagcac ttcttctgcc aaactcggaa
     3721 catttccttt ttcttttcct atcagactga gtagctgcat ggtagcaaat ccgaaattac
     3781 ttcctttcct ataagcaaaa taacgtcctt cccaatgatc gacatatttt aatttctttt
     3841 cataagcaga caggaaggaa tagaaaaaat taccataatt atatacgata tgcatgactt
     3901 tttcttgaaa gaaagaaaaa tctgtttctc caacattagc taatggagct aagcctagat
     3961 tggcttccga gtagcccatt ttctcgtata caataaacag attggctaac agatagtcag
     4021 ctaattcctc agattcttta ggtaagaccc gcaacaaatc atatcccgcc tgcttcttcg
     4081 tcatcggctg agcggtgatg aagccaacga ctttttgatc tttacggaca ataccgacac
     4141 cgctagataa tacatatgat ttatcaaatc gtcccacaga gaagtatttt tctcgtgcac
     4201 cttgaagcca tttttgtgaa acttcattta cagcttgatg taattcttcc ggcagttgat
     4261 cgtaccaagt aaaatggtat ccttctttca tcaaacgttg atattcaagt tgctgactca
     4321 gccgagacaa gtcttctgtt gctagatcaa caatccctgc ttcacctact ttcgtgaagt
     4381 gaaaacccaa ttcatgaagc atcactacgt agctttctga tatcttataa aacgcaagct
     4441 gatagcctaa acaatctgct tgtttcataa actgattcgt tgctgcccgc atcattttag
     4501 gattgccaac tggatctcct aaaacaaagc atttgtttcc tttgatctga taaccaaaaa
     4561 cgacttgatc ttggtcgtct tcttggtaat aaaaataact gtaacctgac aagcgaagct
     4621 gatggcttgt agctgtgcct ccgtatgttt ccaataaacg gtcaaaacgg tctttttcgt
     4681 actcactgcc aagcttcgta gtatcttcag ctaaatattg ataaagcgcc actaaagcta
     4741 gcaatgagat gcccaaacca ataagccctg agaaccacac atcatctgaa ggaaacaaaa
     4801 taaaatgatt tcctgttatt cgaccgacta aaaagcgtcc gctgtgatag cctgcaattg
     4861 cgtaaacgat aaacaaaaag ccgaacaagc tagcatcaaa gattatagct ccccaagaat
     4921 aaacgaattt tgttcgataa aattcttttc tggataacca cacagctaac agaatcaaga
     4981 catataccaa tatcaactgc caagataacg tacgtgtgat tgtatttaaa ataccaaaag
     5041 atagtaaagc aatcgtaggc agaaatgcct ttttgacttt tactgaaagt gctctagcaa
     5101 ggccgagtaa taaaaagcca accaatagat tcactgtctg atctaaaaaa ttgaatgaga
     5161 acggcaataa tacttgaaat agccgactga ttgcagacag attagtgatt gttgatagaa
     5221 gtaccatcat gatccccgca aaatagacag cacttacgag cacgatatgc gcaacctttt
     5281 ggaaaaacag tcgcggaatg ttatctaaaa atcgattgat tttgactcct gtatgtgtga
     5341 tgaacaatag aagtcccgtc ataaaaggta gaacataata aaacaaccga taatataata
     5401 gccaaactac tgctacatct tgccgaacag gcaattgact cattcctaag atcatcaaaa
     5461 cgtcaaaagt ccccattcca cctgggacca tcgtcagcat tcctatcaat gtagcaatca
     5521 tgaacatagg atagatcgaa agcatcgaaa tatctattct catcaacttg ccgatgatca
     5581 aaaataccgc aagggctccc gtccattgtc caaaggatgc cagtagcaga ccaagcaagc
     5641 cttttggata aaactcgaag aacaatgtct ttctctttag ataagcaaaa aagaaaatga
     5701 ctggtgctaa aaaactaccg ccgaaaagcc agatccaata gcttcgaaaa atactttctg
     5761 actgaataaa aaagacatca ataaatgtaa gaaacgacca tacagacaaa ccagtcagca
     5821 taaatagtgc gacttttgat acagtggcta aaaccatttt tctatttgtc tgctttccat
     5881 aaaaactagc ccgcagactt gctcctacca caccaccaaa accggcgaga ttgttgatcg
     5941 tgtttgttac ccacgcagca agaagaaact cttttctttc catttttggt tttccttgtt
     6001 tttctaagac tctgatcgtc acccaatcat acaacaacat cggagtgaca cccagcagtc
     6061 cagccagaat catgagcagg atcgtcatgc ggctttgttg tcccatcctt tgccaaatat
     6121 cttgccaact catgccatta gcaatatttg cgacttgatt tgccacaaag actaacacgg
     6181 agccgaaaaa aatcagtttt aaaatcagac tatgttcctt tatccattgc agcatcaagt
     6241 atcgtttatt tttcacattt tcccattcct aacttgtata cttttgtgtc ataccccaat
     6301 gttatcttat ttattttaat ttttccactt tagacctgtt ctttgcttgc gatgatagaa
     6361 aaagaaagat atcctaatgc taaaggagag gataacatga aagtaggatt tatcggcaca
     6421 gggaatatgg cacaagctat tattgatggt ttattaaaaa aaggaacagt ctcgaaagaa
     6481 gaggtgttcg tttccagtgg acattttgaa aatgcacaaa aatttgctgc tgaaactggc
     6541 gtccatgcat gcaaaagtaa tcaggaagtt gcatcacaat cagatatcgt tcttttagcc
     6601 gtgaaaccaa aaatcattga aaaagtattg gaagaactaa gagaatatag ccagaaagtc
     6661 ttattcattt ctattgcttc aggaaaatca ttaacagatt tagaaacagc gcttgaagat
     6721 cctcaggcct ccatcgtacg tgtgatgccg aatgtcaatg tcagcgtagg agcaggcgtt
     6781 tctgcgattt gcaaaaatga agctgccacg gaggaacagc ttcagcaagc tacgatgatt
     6841 tttgaagcaa tcggcagcgt ctatccttta gcagaatcag atttcagtac attcattggc
     6901 cttgctggca gctcacccgc attcgtttac atgctgatcg atgcgatggg acggactggt
     6961 gtactgaatg gtttgtccaa acagacagcc actgagattg ctgcaaaagc aattatgggt
     7021 tcttgtcaaa aattcctttc aagtgataaa aatccatggg aacttgtaga ccaagtttct
     7081 tctcctggcg gtacaactgt tgcaggtgta gttgccttag aagaagcagg attcatccca
     7141 gcaatcatca aaggaatcaa tacgacgatc gagaaagatc ttgagatgtt gaataaaaaa
     7201 taatgatagc aatgtttttt cgtgccttac aaaaaaagaa gagctgtggc ttttgccgcg
     7261 gctcttcttt tgtatttttt attatttagc ataatcagtt gcacgagttt cccgaataac
     7321 gattactttg atgtgtccag gataatccag ttcttcttcg attcgtttac gaatatcacg
     7381 tactaaacga acagcgtcta aatccgagat ttcttctggc ttgaccatta cccgaacttc
     7441 gcgtccagct tgaactgcgt aacttgattc tacaccggcg aagctgttcg aaatattttc
     7501 cagacttctt aaacgacgga tgtagttttc taatgattca cttcgcgcac caggtcttgc
     7561 tgctgagagc gcatctgctg cagctacaag gacagagata acagaagtag cttccacatc
     7621 tccatgatga gaagcgatcg cgttgattac tactgggttt tctttgtact tggcagctaa
     7681 ttccgcaccg atctcaacgt gagatccttc aatctcatgg tcaagtgctt tcccaatatc
     7741 atggagcaac ccggcacgtt ttgctaattg gatatcttcg ccaagctctg ctgctaagat
     7801 accagctaat tttgcaacct cgaccgaatg cttcaagacg ttttgtccat aactagttct
     7861 gaatcgcaga cgtccgatga tcttgattag atctggatga agtgtatgag caccgacttc
     7921 aaacgctgcc tcttccccgt attcgcgaat acgttcatcc atttctttac gagatttctc
     7981 gaccatttct tcgatacgcg ctggatggat tcgtccatct tgtatcagtt tttccaaggt
     8041 catccgtgcg atttctcttc ggattggatc gaatccggaa agaacaactg cttccggtgt
     8101 atcatcgatg attaaatcga taccagtcag cgtctcaagc gttcgaatat tacgaccttc
     8161 tcgtccaatg atacgacctt tcatttcatc attcggaagg gtaacgactg aaacagtcgt
     8221 ttctgaaact tgatctgctg cgcagcgttg aatcgctaat gataataagt ttttcgcttt
     8281 gcgatctgct tcttctttgg cacgctgttc agattctttt accattagag ttaactcatg
     8341 attcagctct tcttcagtcg atttcatgat gacatctcgt gcgtcctctt ttgataaacc
     8401 agcaattttt tctagttctt tttgttgatc ttcaatcatt ttttcaacgt ctttttctcg
     8461 ctcttcaatt aattgctgtt ttgaactaag tgcttcttct ttactttcaa gtgagttctc
     8521 gcgtttttct aacgagtcat ctttacgatt cagcgtttgt tcgcgttgaa tcaaacgatt
     8581 ctcttgagat tttaattcta acttactttc tctcagctca ctttcaactt cagaacggta
     8641 tttctgattt tcttccttag cttctaacaa tgcttctttt tttagagttt ctgcttcttt
     8701 ttttgcacta tcgatgattc cagcagctga tgattgtgca ccagctattt ctttctcgtg
     8761 acgtgacttt gcgaccataa accctaaacc aagaccgaca attaaaccga tgatagcgag
     8821 gagaatgttg aataccataa aatccacctc catactatct ttttcatttt tatcatctat
     8881 acagcttcta tgataaatta ttctaaactc gtcaatatgc atcttcgcat atatgtgtac
     8941 aaaaaacatg cacaacttta ttctaatgtt tgtaccccgt agtgtcaact ttaaataaac
     9001 aaagaaacgg ctttctactt attcattaaa cgatggtttt taaaataaat gacactagtt
     9061 tcatataggt aaacctttaa attgagaaac ttttctatta aattattctt tgtagatgaa
     9121 cagatatttt tattttatgt acccactcgt ataattaaat aactcacgat ttctttattc
     9181 aataaaaaaa cggcaggtca aggagagtac ctactcttca gaccagccgt ttttgtttat
     9241 cataattttt attcttctac taaaggcaat tcttcttgtc ctttttcatc ttctgggaca
     9301 gcgacttctt caccaatacc ataagctgct ctaactagag cagaaacttc tgccatcatt
     9361 tcaggatgat tagccatata gattttagcg ttttcccgtc cttgaccaat ccgatcttct
     9421 ttataagaat accaagcacc gcttttatca acaatgtctt tttctactgc catgtctaga
     9481 agttctcctt cttgtgagat ccctaaacca tacatgacat ctacttcagc gattttgaaa
     9541 ggaggtgcta ctttgttttt tacgactttg atctttgtac ggttaccaac aatatctgta
     9601 ccttgtttca attgttctgc acgacgtact tccaaacgga tcgttgcgta gaatttcaat
     9661 gcgcgtcctc caggagtgat ttctggattc ccaaacatca cgcctacttt ttcacggatt
     9721 tggttgatga aaatagcgat tgtttttgtt ttgttgatcg aaccagagag tttacgcaat
     9781 gcttgagaca tcaaacgtgc ttgtaacccg acatgtgagt cacccatttc gccgtcgatt
     9841 tctgctcgtg gaactaaagc agcaactgaa tcgactacta cgatatctac agccccactt
     9901 gatactaaag catcagcgat ctctagacct tgttctcctg tgtcaggctg tgaaagaagt
     9961 agttcatcga tattcacacc taatttttgt gcatattgcg gatctaacgc atgctcagca
    10021 tcaatgaaag cggccgttcc gccatttttt tgtacttctg caatagcgtg tagtgcaact
    10081 gttgttttac ctgaactttc aggtccatat acttcgatga tacgtccacg ggggtaaccg
    10141 ccgacaccta aagcgacatc taacgcaaga gagccacttg gaattgtaga gatttgttgg
    10201 tcgatttttt cgcccaattt cataatagag cctttaccat agctcttttc aatttttttc
    10261 agtgcagcat ctaaggctgc tttacgatca tctgccaata cgataatcct ccttatattt
    10321 acaaatcaga gcaacatttc attatcctta tcatatcttt tttagtgaaa aaaagcaaga
    10381 aaaaaccgaa caaatattcg catttatcat tttcttaaaa gctgttttct caaaagatca
    10441 aatccttgca taaccgcatt atatctgata gcagcacgat ctctggcaaa gtgatatttt
    10501 tccgaaacca cgccgtcttt agatgaaata gcgatccata ctgttcctgc agattgacct
    10561 tctaacgtgt ccggtccagc cactccggtg aaagctacac tgtaatctgt tcttgctatc
    10621 tcacgtgctt gaatggccat ctgctccaca cattctttgc tgactgttcc atattctgct
    10681 aatagctccg gatcaatctc cagaaaacca gcttttgttt ggagcgagta ggtaacgaat
    10741 ccgcctggaa atacttcaga tacaccagca atgtcgccta atgcggcttg gaaggcgccg
    10801 gctgtcaaac tttccgctgc tgttacagtc tgcttgtttt ctttcagtag ttccactacg
    10861 acttttgcta gcgaattatc atcgccgtat ccataaaaat attcccctac tcgttcttgg
    10921 attttttctt ctaatgctaa aagagcttga ttccctgcct gaacatcatt tgttttcact
    10981 gtcagtcgta atgtcacttc atttggtttg gcatatggtg cgatcgttgg attgatctgt
    11041 gtctcaatca gatcttttag ctcagtgacc agtcgcgatt caccaatccc ataaaagcgc
    11101 aaaactttag aaattagttt ttcttcagaa ggaaaacgtt cctctagtaa aggacgtact
    11161 tgttccacaa acattggttt caactcatta ggcggaccgg gaagaaggat atacgcatgt
    11221 gtatctgttt gataaaaagt ccctaaagca agccctgtac gatttggtag aggaaccccg
    11281 ccttcgataa tctgggactg ttgcagattg tttttggtca tttttcgatg ggttgtttca
    11341 aaatatgcca ataatttttt gtatccttcc gtattttgta tgagagactt accgaggtga
    11401 gccgcagttg cttcttttgt caggtcatct tctgtcggtc ctaatccgcc acataagagt
    11461 atgagttcac ttcttgtttc agctagagca atcaactctt ctaaacgctg ctgattatct
    11521 cctacaactg tttgataata aacttcgata cctagatcag ctagctgttc tgacaagaaa
    11581 gtggcattcg tattcacgac ttgtcctagt aaaagttctg ttccgacagc tattatttct
    11641 gctttcatta cttcccgctc ccttatatag tatacgctgt atttttatat ttttttattt
    11701 aaaaagaggg caagg
//
LOCUS       DAIGOK010000088        11412 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.088, whole genome shotgun sequence.
ACCESSION   DAIGOK010000088 DAIGOK010000000
VERSION     DAIGOK010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.088"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(88..1383)
                     /gene="purB"
                     /locus_tag="NOA01_001943"
     CDS             complement(88..1383)
                     /gene="purB"
                     /locus_tag="NOA01_001943"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCI0633127.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1396..2520)
                     /gene="purK"
                     /locus_tag="NOA01_001944"
     CDS             complement(1396..2520)
                     /gene="purK"
                     /locus_tag="NOA01_001944"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCI0633128.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2513..3001)
                     /gene="purE"
                     /locus_tag="NOA01_001945"
     CDS             complement(2513..3001)
                     /gene="purE"
                     /locus_tag="NOA01_001945"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCI0633129.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3417..4724)
                     /locus_tag="NOA01_001946"
     CDS             complement(3417..4724)
                     /locus_tag="NOA01_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HCI0633130.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4727..5308)
                     /locus_tag="NOA01_001947"
     CDS             complement(4727..5308)
                     /locus_tag="NOA01_001947"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCI0633131.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5424..5521)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5880..7172)
                     /locus_tag="NOA01_001948"
     CDS             complement(5880..7172)
                     /locus_tag="NOA01_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCI0633132.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7169..7792)
                     /locus_tag="NOA01_001949"
     CDS             complement(7169..7792)
                     /locus_tag="NOA01_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633133.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7795..8631)
                     /locus_tag="NOA01_001950"
     CDS             complement(7795..8631)
                     /locus_tag="NOA01_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCI0633134.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8648..9535)
                     /locus_tag="NOA01_001951"
     CDS             complement(8648..9535)
                     /locus_tag="NOA01_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCI0633135.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9551..10834)
                     /locus_tag="NOA01_001952"
     CDS             complement(9551..10834)
                     /locus_tag="NOA01_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCI0633136.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
BASE COUNT         3440 a         2565 c         1910 g         3497 t
ORIGIN      
        1 attttgtaca acccatcagt ccttattgac aaagagccgg ttatttttgc tattctattt
       61 taattgaatt ttgcaactca ctcttcttta ttccaaacct actcgtttga atatcacgtc
      121 aacgtttttc aaatgatgat gataatcaaa ggcatcgtct atttcttctt tagataatac
      181 cgatgtgatg gttggatctt cttctaacag cgggcggaac tgcacttggt tgtcccaagc
      241 gtacgcagtt tttggctgaa caagatcata agcttcttct cttgtcattc ccttatcgat
      301 caatttcagc ataacgcgct ggctataaat caaaccaaat gttgcagaca tattccgctt
      361 catgttttca ggaaagaccg ttagattttt aacgatattg ccaaaacgat ttaacatgta
      421 attcaataaa atcgtggtat caggcaagat gattcgttct gctgaagaat gagaaatatc
      481 tctttcatgc cataatgtga cattttcata ggcagtgacc atgtgtccgc gcaccactct
      541 ggctagacca gccatatttt ctgaaccgat cggattcctt ttgtgcggca tagcagaaga
      601 acctttttga cctttcgcaa aaaattcttc tacttcacgt gtttcggatt tttgcaatcc
      661 gcgtatctct gttgcaaaac gttcaatact tgtagcaata agcgccatcg ctgagaaata
      721 ttcagcatgt agatcccgag ggagaacttg agtagagatt tcttgtggac ggatccccaa
      781 ttgtttacat acatactctt caataaatgg cgggatattt gcgaacgttc ccactgctcc
      841 tgagattttt cctgcttcta ctccttttgc cgcatgttca aatcgttcga tgttccgttt
      901 catttctgaa taccatgtgg caagtttcaa tccaaaagta gttggttcag catggacacc
      961 gtgtgtccga cccatcatca ctgtatattt gtgttctttt gctttttcgc caatgatttc
     1021 cgtaaaacgt ttcaagtctt cccgtaaaat atcatttgct tgcttgatga gatagccata
     1081 agcagtatct acgacatccg tacttgttag accataatgg acccattttc tttcttctcc
     1141 caatgtttca gaaaccgctc gtgtaaacgc aacgacatca tggcgtgtta atttttcgat
     1201 ttccaagatc cgatcgatat caaatgatgc atgctcacga atttttttca catcttcttg
     1261 cgggatttca ccaagttctg cccaagcttc atctgctaaa atctcgactt cgagccacgc
     1321 acggtatttg ttctcttccg tccagatatt ccccatttca gggcgtgtat aacgttctaa
     1381 catgtttttt ctcctctaat cccaaatatt cgtttgtttg atttcttcca gcgtttgctg
     1441 gatgtcttct gtcaagatag tgatatgtcc catcttgcga cctttttttg cttgtttttt
     1501 tccatagaaa tggaattgcc agtctggctt attctcaatc aagcgatagc tttccagcag
     1561 ttgttctccc aaaacattga ccattactgc attgcttatc aaacgtactt tttctcctaa
     1621 tggccaacca caaatacctc gaatatgggc atcgaactga ctcatagaac aagcttcaat
     1681 agaataatgt ccagaattgt gaggacgagg agctaattca ttcacatata gcccgcctgt
     1741 ttttgttaaa aacatttcta cccctaaaac acctgaaagg ctcactgctt ctgcaatcac
     1801 acgggcaata cgctcagcct cctcgatcac atcctgatca atcgcggctg gagcaatcgt
     1861 ttcatgaaga atattgttgt ggtggatgtt ttccacgacc ggaaaagtcg tgaaatctcc
     1921 ttgaccattt cctgccacca tgatcgaaat ctctttttca aacgggatcc atgcttctaa
     1981 aacacaggtt ccttctctta aaaggtccat ggcaggagct agatccgccc gacttttcaa
     2041 aacatactgc cccttgccat catagccgcc tcgagtggtt ttcaaaacac aaggatagcc
     2101 gatcccgtca atcgcatctt ggatatccgt cggactgacg atcgttgcat aaggagcaat
     2161 cacgatgtta ttcgtttcca aaaaagattt ttctaacagg cggtcttgcg taatcgcaag
     2221 caagtctgtc ccttgaggaa taaatgacat gggtaaaatc gcatttaacg cctctacact
     2281 gacgttttcg aattcatagg taatgacatc tgttcttctg gcaagctctt tcagcgcaaa
     2341 ggtgtcatca tagacacttt caatgtgcca atctgcaact tgtgcggctg gacaatcgct
     2401 cacaggatcc agcacaccta ctttaaagcc catttctttt gcactaatcg ccatcatacg
     2461 acctaattga ccgccaccga cgatcccgat cgttgaacca ggcaacaaag gtttaaccaa
     2521 gttgatcact actttctata acagttttag ccaacattga acgtctttca gccaatttag
     2581 cctccaattc taaatcatac atggaaagca tctgtatcgc aagtagtcca gcattaattg
     2641 ctcccgcttt tccgattgct gtagtagcta caggtactcc tccaggcatt tggacaatcg
     2701 aaagaagcga atccaaacca tttaatgttc gtgattggac cggtacaccg ataaccggca
     2761 aagttgtttt tgatgcgacc atacccggca gatgcgcggc tccccctgct cctgcaataa
     2821 tgattttgat tccttttttt cgagcgtttt gtgcatattc aaacatcaaa tctggggttc
     2881 gatgagcaga aacgaccttt ttttcataag acacaccgaa ttcatccaat atttgacatg
     2941 cttctttcat tgtttcccaa tctgaagtac tccccataat cacagagatt tttgctgtca
     3001 ttgtttttcc tcccagtttt tctacattca tattttaaca atcacttttt ttgatgtcaa
     3061 agaaatttcg aatgttaaca aagaaaaaca ttttattgtt cgtgtttatt aaagataaaa
     3121 aaaaggaatg tgacaaaagt taggtcacat tcctttttat ggataaacgg tgttctcctg
     3181 ccaactcctc ggtcacaagg aacaatattc aacaaacatg agaagctttt ttttgaaatt
     3241 gttccttgtg actcggagat gaacggcagg ctcacgacct cttccttcaa cggtgttcaa
     3301 tcagctgacc ctcggtatta gctcaaacaa acggcaaaat acgaggaact atttttccgt
     3361 ttgtttctct aacaatcagg tcaaaacgct gatttcacaa cctttctttt gctctattac
     3421 ttcttgaagg cttcttccaa tatttcttca tcttttttca atccattaaa gatcaaattc
     3481 aatatgatag ccgtaatgct actcattacg atgccatttc cagtgaacat ttgcaatgtt
     3541 tgcggcattt gactgaataa tgtcggcgta acattgaaac ccagcccaaa tccaacagaa
     3601 acagcaacaa tcaatagatt tttatcattg ctgaaatcaa cttccaacag catacggatt
     3661 ccttggactg ccaccatccc gaacatgact aacattcctc caccaagtac cggctcaggg
     3721 atgatttgtg ctaacgcacc gattttaggg aataatccta aaacaatcaa gaagaatgca
     3781 gagaaataga tgggttttct tgttttgatt cccgaaagtt gaaccaatcc gacattttga
     3841 gaaaagccag tataagggaa tgtattgaaa ataccgccta agataacggc aagtccttct
     3901 gcacgatatc cctttttcaa ttcttcttct ccaacatttt tcccagtaat gtcaccaagt
     3961 gcaaaatata cacctgttga ttctaccatg ctgaccatgg aaatgatgat cattagtaaa
     4021 atcgaccata cgtcgaaagt tggtttacca aaataaaagg gttgtggaac atggaacaac
     4081 ggcgcatctc caatcgcgct cagatcaatc attcccatga aagcagctaa gatgcttcct
     4141 ccgactaaac caatcaatac cgcgattgaa cgaatgaacc cttttgccag catttgcacg
     4201 cctacaatta ggagaatcgt cacaaatgca agtagcagat ttgttttgtc accaaaatca
     4261 gctgctgttg cattacctcc acccattttc tcgattgcta caggaatcaa agtcaaacca
     4321 ataacggtga taaccgttcc tgttactata ggagggaata gttttttaat tttagaaaag
     4381 acaccagaaa ctaacacaac aaagatccca gaagcgataa tagatccgta gatagctcct
     4441 actccttcat tggttccgat caagatcagt ggagctactg cttggatcgc acatcctaaa
     4501 acgacaggca agccaattcc gaaaaatcgg ttaactgtca attgcaataa tgtcgctaat
     4561 ccacacataa agatatcaat tgaaatcaaa taagtcatct gttcttgatt aaagcctagt
     4621 cctgtaccga tcaaaagagg caccgctact gctcctgcat acatcgctag cagatgctgc
     4681 agccctaaaa ccgcagcttt tccattttgt gtttcttttt ccacgattat gcgtcctcct
     4741 caagaaactc aacttgtcca ttcgtcaagg aagcaatacg cgcaagagaa acgactctca
     4801 atcccatttc ttcaagtaac tgacgtccat cttggaaaga cttttcgatg acgatcccga
     4861 ttccttctac ttgcgctcca gcttgctggc ataattcgat caatcctttt gccgcttgtc
     4921 cgtttgccaa gaaatcgtcg ataatcaata ctttgtcttc tgaagataaa aatttacgag
     4981 aaatagaaat cgtacttgtc acttgtttcg taaaagaata aacagaagct gtcaataatt
     5041 cttcatccat cgtcaaactt tttgcttttc tagcgaagat cattggaaca tctaattctc
     5101 ttgcagcaaa taaagctggt gcgatcccag aagcttcaat ggtcacaact ttcgtgattc
     5161 cttgttcttt aaatacttct gcaaaccgtt ttcccatttg ttccatcaat acagggtcta
     5221 cttggtgagt gacgaagctg tcgactttta atacgccctc gcctaatata cgtccgtctt
     5281 tttgaatacg ttcaactaat tctttcactt tatttgctcc tttatcattt attaataccg
     5341 aaataaccgc ttttgtgcaa aaacctacta tgtttctgtt aaagaaaaac accctccact
     5401 ttatcataag aaaagaaggg tgaaatagcc aatcttttac ttatagtcca gcatttacgg
     5461 tactggctag aaactgtcat ccatattatg acgatatata agtaggtatg taaaactaat
     5521 aatagtgtaa attcattttc ttgcaactgt tttgcacgtg aaggcagttt gccatatttg
     5581 aataaaaaat gttgtttcaa atattgaaaa cagtctaaca ctactttctg aaactgacaa
     5641 cgtggtttac ttattttttt gtaaaatcta atgattttta atgcattatt cctttttgtt
     5701 cgggtttaaa tagttatttt tattttctcc tatctaaaaa agcagcccac ataaaaagaa
     5761 ggcatgacaa aaagcatgtc atagcccctt tttccgaata aatagtgttc aaaagacagc
     5821 ctatcggcaa taaggcaaga ttttataaac tacgggaagt aatttccgta aatctattct
     5881 tattctttta agctgacaag ctcgtcaatc actactgctt ttcctgaagc aatagaatga
     5941 tttgctgcga ttcctgtcaa aatcgacaaa ataccatcta cgtgagttgc tgcccgattc
     6001 cacggatctt cttctttttc ttcaccaaat agatcatcta ataaacgcgg atctcctccg
     6061 ccatgtccgc ccgcttcttg ttcgacgatt acctcatatg gctcaccgaa catcggatag
     6121 acatggatgg actgaagaac ggctgctcct tcatcctttt tatctccacc tgcattgatg
     6181 taagactttt cgatcacttt catttcgatc cgtcctttgc tgccattgaa agaaacattg
     6241 aatccttccc acggcatata cgcgtttagc gaatacgtca aaatcgcacc agtttcatat
     6301 ttgaccgtta cgcctagtgt atcttcaata gagatatcat cggaaaatac actgcggtca
     6361 cgaatatatc cactgtcttt ttctgcttct aaatacattt ctcgcaattc ctcattggct
     6421 gacaggtcta tagcaaatgg atcagcttta gctagttcgc ttccatagca tcgactgtaa
     6481 aatatttcta ctccgcgttt ctccgcatta tgcatcccat aaaagtttaa tccaccgaat
     6541 gcaaatacct tttggggacg gctgttcaac caaaaattca ctagatcaaa atgatgggta
     6601 gatttatgaa caagaagccc gccgctattg tgcttatttc gatgccatct tctgaaataa
     6661 tctgccccgt gttgcgtatt taacagccat tcgaagtgga tagaaaacac atcaccaatc
     6721 acatcacttt ctatcagttc ctttatcttc gtgtgatacg gtgcataacg ataattaaaa
     6781 gtgactcgca ggcttcttcc cgtctgtttt tggaccgtta ttatttcttg agctttttcc
     6841 tcatcgatag tcagaggttt ttcgcaaata acatcatatc cttttttcat tgcacgaatg
     6901 atataatcat gatgcgttcg atctactgac gtaataatga cttcagccgg ccgtacgtca
     6961 tccagtagtt gctcaaattg tgatggcata catgtcttta cttttttctg tccatacttg
     7021 ttcacaagta tccgatttgc ataatccatc cttgtttgag atacatcaca aaaaccgact
     7081 agttcacaag tttgatgata cgtagaggca attgcctcat aaaaaaaacg cgctctccca
     7141 ccaagtccga cttgtacata ttttttcttc atttaaagtc attctccttt tcttttaagc
     7201 aaaatactgt gacaaatggc agccctggat acagtaatgc agtgagtaaa aaatcatgct
     7261 gtatacacca gaacagtcca tacaaaaggc ataaatacca cagaaacccc ttgacgtttt
     7321 ttgacaacag ccagatattc cttttgatca aataaacaag actatctgct ttatcttctt
     7381 tgcagatatc aattagcgta ctaaaaaaac tatgggatac aaaaagcaac agacacaata
     7441 gaaaaggaat cagataccaa gctccaatct ttcccactaa taaataccaa tcaactgcaa
     7501 aaataatgac aaacagctga aacaacgtaa tgatgaaaca catccgtttg atttttcctt
     7561 tcctccaaca tacctttttt tctgccaagt aatggacaga tagataataa gaaggataca
     7621 tcatcaaaaa ataaatcaga tagacaacac gagcagacag cactaatcct aaagtgaaca
     7681 aacatcctag aactaaagcg aaaagagcat tactgataag actggttaaa aaaatccgac
     7741 agacatagta gttgagcttg taaagaaaca tctcttctat tggctttttc atattcagcc
     7801 cttcagaccg cttgtactga tcccctggac aagatattta ttgaagaata agaaaatcag
     7861 aaaaaccggt agcagcgaca acgtagacat cgcaaacata ctcgcgtaag tcgtactaga
     7921 tccagcgtct gcaaataatt tgatggcaat cgataccgtg taagatctcg gcttgtttaa
     7981 ataaatcaat ggtcccatga agtcgtccca tttccaataa aactggatga tgatcgtcgt
     8041 gatgatcgaa ggtttcagca aaggaaaaat aattttggta aagacactat acttgctgca
     8101 tccgtcaata atcgctgatt catctaactc tttggggatg ctgaccatga attgcatgat
     8161 ctggtaaatg aaaaatgctt gtccaaaaaa atgtggtaaa atcagcggca catacgtccc
     8221 aaccaaattc agccgattgt aaataataaa ctgcggaatc aaaatcactt gtgcaggaat
     8281 catcatcgtt actaacatga cgacgaacca gaatcttttt cctttgaaat cgattctcga
     8341 taatgcgtaa gcaatacaag tcgagctcag aactgtccct atcgtcgaaa ctgcagagat
     8401 gacaaacgaa ttcctaaaga aagtaaaaaa tgaaattcca gcaaaccccg cccatccttt
     8461 tgcgtaattt tcccattgaa aagtctctgg aatcaaattc agcgaaccac tcataatctc
     8521 ttgatccgtt ttaaaagaac ctgcgatcat ccataagacc ggataaatca tgactagacc
     8581 taatagcaaa ataaaaccgt attgaaacca tttcgttact gtattattca tttcttcctc
     8641 ctctcattca cacaacgacc catttttttg atgatttgaa aatcagaaag gtgacagctc
     8701 ccataatcaa cagcatgatc cacgaaatag cactagcata ccccatagaa ttaaaagaaa
     8761 aagcttggtt atacacataa agagcataaa agttcgtggc atcattcgga ccaccttggg
     8821 taatgataaa tgcctgcgta aaagtcataa acgccgcgat tgtctgcata accaagttat
     8881 acaaaatgat cggcgaaaga agaggtaacg tgatcttgta aaacgtctgc catttgttcg
     8941 ctccatcgat ctctgccgct tcataatacg agcctgggat ctctttgata cctgcagcaa
     9001 agatgatcat tgccgaaccg aattgccaag cagacatcaa aacaagaggg atcatgacca
     9061 gtttttgatc gctaaaccag gaaagcgcag gtgcgcctaa tcgttccagg aaaatattca
     9121 ctaacccgtt tttagaaaac aactcccgcc aaactagagc gatagaaata ctgccaccga
     9181 tcaaactagg cacataatac aaagaacgaa agaaagtcgt tcctttgata ttcctcgtta
     9241 aaagataagc aatcaataga ccaaagatca gtttcacagg tacagatgca aatacataga
     9301 gaaaagtgac tcggatggag ttcaaaaaac gggcatcact cagcatcttt atataattat
     9361 ccaagccgat ccatgagact ggatttccca aacgataatc ggtgaacgaa taataaagtg
     9421 acaagatcat cggtatcact gttatcaata gaaatcctag gatgaatgga gaagcaaata
     9481 cgtaaccgac cacattgtct tgatccaaga accgttttag tttttctttt ttcatgcctt
     9541 cctcctgctg ctatcctaga atgtttgacg caaactcata aagactctct gctgcttctt
     9601 ctgctgttac ttcttcataa atgacttttt caactaaaag attgtattgg tcttgaattt
     9661 ccggattcaa agcactatca aagacattca cttcgccgtt gtccatatcg gaaatcattt
     9721 ctaaataatc aaatgtcgct tgcaaagttg catcatctgc acgagcgagc tgttctcgaa
     9781 tattcgacat gattggtaca ccacgttctc cttttaaaat ttcattggct tcttcactgt
     9841 tttgaaagaa atcaataaac ttagcagctt cttctttatg ggctgagctt tttgccatac
     9901 tcagcatttg cgaagaacgt aaagggatac cagattctcc atctgctttt ctgcgaggtg
     9961 gattaatcaa ctgaatctcc atatcttcag gagcccctga caatatttct gtcaactggt
    10021 tacttgatag aaacaccatt gccgcttctc tatccctaac cgcctgaaaa gacagatccg
    10081 tcgtacttgc agctccacca cctggttctg gatgggcccc tttgtctacc agatttttac
    10141 gcagctggaa aaaatctgtt agatagtctg gattttcaaa tcccaagctg ttagatagat
    10201 cttcccgatt ataaaaattc aatcctttct caacttgtgg tatttgaata atacagccag
    10261 ctataaaatc ttcgaatctt gaaatagcat agattcctaa ttcttcagtg atttgatagc
    10321 tgatttgttc aaattcatcc catgtccaat ttgaagctgg ttcagggata tttaattgtt
    10381 caaatatatt tttattgtaa gcaatcgcaa acgaattcac cccgttagac agtccaacta
    10441 attgcccgtc gtaagtcgtc gttgctaaat aggattcatc tgcattagaa atatcaatta
    10501 tcccttgctc cacatattca tctaatggtt caatctgatt gatgtattga ggaaattgat
    10561 tgttcagttg gaaaatgtcc cagatatcat ctgatgctgc tagtgtacct aattttgtgt
    10621 aatatccttc ataatcataa aattcataat cgatcgtcac atggggattt ttttcttcat
    10681 aaagctcgat tacttttact gttgcatcgt gtcggacttg cgaaccccac caagccatgt
    10741 tcagcgtgat ttttttgtca tcttcttgcc cacaagccgt caagaagaat aacataacac
    10801 ttaaagctaa gatgattctc attcttttct tcattctctg cacctctact tccattgtta
    10861 tttttcaata tactgtattt ttatacagaa catatttatt aaacgatctt tttcaagggg
    10921 aataaaaacc aagacttgcc ttatcgttct gcctaggatg aacaactcaa tactttcaag
    10981 aaaaattaga tagtgctgat tcttctttca tcgctaaaaa cacttcttgc ttcaatgatg
    11041 ggaggataat tacgcaaaga aaaaaggata tcagacaact gcccaatatc cttgacatag
    11101 ctaaataagc atgcttcttc gtgtgcgctc ttccctaaaa aacagaaaaa taactagtaa
    11161 ttctgtatga aattaggtac aatatacacg tcgatgcaca agcattgact atggcaactt
    11221 gattggtcag ttcccgctga cttttcaagc cccgacaatt tagctgctac taaattgtcg
    11281 ggtgcctttt ttagttttta ttcgtttttc attctataga aagcgcttca ataaatcaag
    11341 acattttttt gttagaatgc taataataac aaaaggtttt aactcttctt atgtacatta
    11401 caggtaaaaa aa
//
LOCUS       DAIGOK010000089        11283 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.089, whole genome shotgun sequence.
ACCESSION   DAIGOK010000089 DAIGOK010000000
VERSION     DAIGOK010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.089"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            22..1653
                     /locus_tag="NOA01_001953"
     CDS             22..1653
                     /locus_tag="NOA01_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HCI0633137.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(1715..2356)
                     /locus_tag="NOA01_001954"
     CDS             complement(1715..2356)
                     /locus_tag="NOA01_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCI0633138.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    2542..2618
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            2638..2946
                     /gene="rplU"
                     /locus_tag="NOA01_001955"
     CDS             2638..2946
                     /gene="rplU"
                     /locus_tag="NOA01_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCI0633139.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            2973..3317
                     /locus_tag="NOA01_001956"
     CDS             2973..3317
                     /locus_tag="NOA01_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HCI0633140.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            3330..3623
                     /gene="rpmA"
                     /locus_tag="NOA01_001957"
     CDS             3330..3623
                     /gene="rpmA"
                     /locus_tag="NOA01_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCI0633141.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            4057..6864
                     /locus_tag="NOA01_001958"
     CDS             4057..6864
                     /locus_tag="NOA01_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCI0633142.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            7055..7546
                     /locus_tag="NOA01_001959"
     CDS             7055..7546
                     /locus_tag="NOA01_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCI0633143.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            7649..8638
                     /gene="manX"
                     /locus_tag="NOA01_001960"
     CDS             7649..8638
                     /gene="manX"
                     /locus_tag="NOA01_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCI0633144.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            8671..9474
                     /locus_tag="NOA01_001961"
     CDS             8671..9474
                     /locus_tag="NOA01_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCI0633145.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            9495..10409
                     /locus_tag="NOA01_001962"
     CDS             9495..10409
                     /locus_tag="NOA01_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCI0633146.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            10492..10866
                     /locus_tag="NOA01_001963"
     CDS             10492..10866
                     /locus_tag="NOA01_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HCI0633147.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            11005..11250
                     /locus_tag="NOA01_001964"
     CDS             11005..11250
                     /locus_tag="NOA01_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCI0633148.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
BASE COUNT         3550 a         1914 c         2495 g         3324 t
ORIGIN      
        1 catgaaaaag gagttagata ggtgttcaaa aattatgttt cctcactaaa aaaagaattt
       61 tcaggttatg atctttcaag atttcagaaa gatcttttgg ctgggatcac agtagctgct
      121 gtagcattgc ctttagcctt ggcttttggg gtatcaagcg gagctgatgc ggccgcaggt
      181 ttgattacgg ctatcattgc cggtatcgtt attggcggtt tatcaggcgg tttttatcaa
      241 atatcaggtc cgacaggtgc gatggcagct atcttgatgt ctattgcggc aacaaaggga
      301 atgcaaggtg tattgcttgc tactttttta gcgggtctat ttttacttgc agcaggactt
      361 ttccgattag gaacactgac ttcttttatc ccagcacccg taattacggg gtttacttca
      421 ggtattgcta tcattattgc attaggtcag atcgataatt tatttggtgt tcactcggaa
      481 ggtacaaacg ttattgaaaa actaagtagt tatcaaacga taggatttcc gataaacaca
      541 gcctctttga tcatgggagg gctagtcatt ttagggatgt ttatctatcc aaaaaaatgg
      601 gcgaaacgaa tgcctagttc gctgctggct atcattttgg caacagcagt tatgctattg
      661 ttccatttac cagtagcaac tgtgggtaag attccacaaa cattgatcag tagtaatcgt
      721 ttgaatatgg gagattttag cttttctgcc ttgcaacaag tggcagttcc agctattagc
      781 attgctttat taggaatgat tgaaagcttg ctatgtggtg cttcggctgg ccgaatggca
      841 aataagccct tagatagcaa tcaagaatta atggcgcaag gaattggaaa tctgttgctt
      901 cctttctttg gcgggattcc tgctactgcg gccatcgccc gtacaagcgt ggccatcaaa
      961 tctggtgcac aaactagatt agctggtatg atacatgcag ttgtcttact gctctccatg
     1021 cttgtttttg cgccagtgat gtcacagatt ccaatgccag cacttgcagg tgttttgatt
     1081 gtgacggctt ggcgtatgaa tgaatgggaa acgatcaagg agctatttac taataaatat
     1141 tggtcagcag ttctattgtt tatccttacg atgatttgta cagttatctt tgatttaagt
     1201 attgctatcg tgatcggggt gatcggcggc tgtgtatttt ttgttgtcaa aagcgctgct
     1261 atcacgattt cattagaaac gatcgattgg caaagaatgg ggctgccaga aacggaaaag
     1321 ctgaataact ggacagtagt gtatattagt ggtccactgt tttttatgtc ttcagaaaaa
     1381 ctcaaaaata aattggaaga actaaatcat cacgatggtg tcattttctc catgcgaggc
     1441 gtaccaagta ttgatttgac ggcacgtgca gtttttgatg aatttcaaga aaaagcagag
     1501 caaagaggac aaacgattat ctatacttcc ttgcagccag aagtagaaaa acaactttct
     1561 cacttgtggg aacatacatt cacgcatcaa catcaaacag ttgctcatgc gttgacagct
     1621 ttacatgcga aatatactgc aattagccat taaaacaatg aaaaagaaca gacaagactg
     1681 taaactgaag attttatggt cttgtctgtt ctttttaata ttgctgtaaa agtgaagcta
     1741 gtacttgcca gtcttgatgg ataacaggta caagggagcg attgtagaaa atagctgctg
     1801 gatgaaaaag tggaagaatt cgatagtttt tttctgacca ttcgtaggca tccttttgtt
     1861 ctgttaattg taagacaggc cctgtataga gttgaccatg atttttggta accgtatatt
     1921 ctttgccaag taaacgttga agaccaatgt ttccaagtgt cgcaattaat tttggctgga
     1981 ccttcatcag ttcgtaatcc aatattggtg catgcgcaaa tacttcggaa cgagtaggtg
     2041 tccgatttgg ataaacagtt tctgactgtt gtgtccgttt attgatccga tgagtcacgc
     2101 gatacgggcg gctccttaca gcactggtga tatacacctc ttctcgtgtt aagccagttg
     2161 aagaaagtgc ctgcatcaat tcttttccag cttgaccgct aaaaggaaca tgcgtatcaa
     2221 tctctgtttt ccctggggct tctcccacga gcatcagttt aggatgaaga ggtccttgcc
     2281 cagcgataaa tccttctaca ggacggccgt tcattcttaa agaagcttct tgtatcagtt
     2341 cttttgggta ttccatttat gctcctcctt tttcttttat tataagaata aaagaaacac
     2401 aatgcgacta ttttatttgg tagaactgtg aggatagaaa agcagaagta ttgcttgaat
     2461 ttttgtttta ctttttgatt ttaaatttag ttgactaacg gttgctgttc tggtaagcta
     2521 gtactgttgt aattgtggac tccacagcta caaccgctca gaacaagtaa taagcacagc
     2581 atatgctgtc acttcggatg gcgagtctaa gtatatcaga aggaggtgct ttatagcatg
     2641 tacgcaatta ttaaaactgg tggtaaacaa gtaaaagttg aagtgggaca agcaatctat
     2701 gttgaaaaac ttgacgttga agcaggagaa aaagttgttt ttgacgaagt tatcttagta
     2761 ggcggagaat ctacaaaagt aggcgctcca actgttgcag gtgcaactgt tgaaggtact
     2821 gttgaaaaac acggcaaaca aaagaaagtc gttactttca aatacaaacc taaaaaacat
     2881 tcacaccgta aacaaggtca tcgtcaacca tatacaaaag tggttatcga cgcgatcaac
     2941 gcttaatagc gtttgagaaa gaaggcctat atatgatcaa aggaacattt aagcgaaatg
     3001 acgcaggtca gatcgtttca ttcactttga ccgggcatgc tgatgctggt ccttatggaa
     3061 gtgatatcgt ttgtgcgggt gtttctgcgt tagcgatcag cactgtcaac gggattgcct
     3121 cattagccgg ttttgagccg attgttgaaa tgaatgaaga agaaggcggt tatctttata
     3181 ccgaagtcac ttcaggaatg acacaagaac aaaacaatat tgctgagatt ctattagaaa
     3241 atcttttatt aggattacaa tcgatagaag ctgaaaactc agaatatatc caaattaaaa
     3301 caattaatga taaataggag gtgcagacca tgttgttatc tatgaatcta caattatttg
     3361 cccacaaaaa aggtggcggt tctacatcta acggccgtga ctcagaatca aaacgtctag
     3421 gtgctaaacg tgctgatgga caaacagtta caggtggatc aattctttac cgtcaacgcg
     3481 gtacaaaaat ctatccaggt gtaaacgtgg gtatcggtgg agacgacact ttatttgcaa
     3541 aagttgatgg cgtagtacgt ttcgaacgta aaggccgtaa caaaaaacaa gtgtctgttt
     3601 atccagtagc tcaagaagct taattgaatc ttatgattaa agaggttgtg acaagacttt
     3661 tgtcacagcc ttttttgttt ataaaaataa aagttgtagt ggaggctacg aagtttactt
     3721 tgtcttttta cacttaatat ttttttgcca atttggaatc aggatgtttt gataactatc
     3781 gttttcaatt gatggaagct atattgtttg ctatcagtga aataattata tggtaaacag
     3841 tgttcttttt cttgtttcca ttataaaaat cgtatcggga tttttcgttc ttttaaattg
     3901 aacatttcta tctatccgcg tgttatcaac aaaacaaaat caggtaaata gttgacgaaa
     3961 cagaaataaa tacatagtat tatgttttga agcctaagta caatagtcta tagaaaagga
     4021 gcgataccat tggcaaaacg cattgaccga atttatatgt atgtcaaaga aaaaaccgct
     4081 catttaacaa aggcagaata tgatcaagga gtaacaacac aggaaattgc tgaagtgtta
     4141 gggattcagc gaacaaatag cagtaaagat ctgaatcagc tagtgcgcga aggaaaactg
     4201 ataaagacag acggacgacc agtacgttac atatatcaaa cactcgttac gcacgtaaag
     4261 ccagttacaa aacatgtagt cagttacaaa gaaacgccgg tctctgaaga aaaaaataca
     4321 ctccaaatag atactaagga tatctttgca aaaatcattg gagctaacgg cagcatgaaa
     4381 aattcagtgg aacaggcaaa agctgcgatt ttgtatccgc caagaggatt aaattgtttg
     4441 attacaggac ctaccggatc aggaaagact tattttgcac acgcgatgta tcattttgca
     4501 aaagcgcatc atgtaataga tgaggaaacg gaactgattg ttttcaattg tgctgattat
     4561 gccaataacc ctgagctttt gatgagtcat ttatttgggt acgcaaaagg tgcttttaca
     4621 ggcgcagatg aagaaaaaat ggggattatc gatcaagcgg atggcggtat gctgtttttg
     4681 gatgagattc accggttgcc gcctgaaggt caagaaatga tcttttactt tatggatcat
     4741 ggaacttaca gtcgtcttgg ggaaacaacg aaatcccatg aagcaaatgt acggatcgta
     4801 ggtgcgacca cagaagaccc aggttcttct ctattagaaa cttttgttcg gcgtatccca
     4861 atcaatatca aacttccggc ttttgaaaaa cgtcctgcaa atgagaaagt cgatttagta
     4921 aaaatcatga ttgcccacga agcaaaccgc acgcagcgaa agatttcttt gacagaagat
     4981 gtagcaaaag cattgatcgg cagtgtgacc tatggaaata taggacaatt gaagtcaaat
     5041 atccagttag tatgtgcacg aggatttcta aatcatatgc aatcacccga aatctcgatc
     5101 acaatcgatg atttgacaga agggattcgt agcggattga ttcaattagc aagcaaccgc
     5161 agtgcaatgg cagagctttc taaaatattg gagccaaaga taactgtagc accaaatgat
     5221 acgatcatga aaatccaatc ggactcctat gagttacctt ataatctata tgatatcatc
     5281 ggagataaag cagctttact gaagtcggat ggtttggatc aagaagcaat caatcatttc
     5341 atttctacag atatcaacat ccatctgaag tctttctata aagaccatgg tttttctttt
     5401 aatgcagaca gcaaattagc agaatttgta gatccgaaaa tcatagaagt gaccaatcag
     5461 atttatgtaa tggtcaaaag atctttacca tatgagtttc agcaaaactt tatttatgct
     5521 atgagcttgc atatcagttc ttttttgaaa aaaatcaact taggagaaga acggcatacg
     5581 aatgataaca tccgtgagat ggcttttgat tttccagatg aatatgctgt tgcgaaagaa
     5641 gtgcgtagtt atatcgaaac gtattttcaa gtggagattc cagaaagtga agactattac
     5701 ttgaccgttc tactcgtctc gttacgggca aatcaagcaa gcggcagaat cggtgtagtc
     5761 gttgcagctc acggaaacag cacagcaagc agtatggtcc aagttgtcca acagttgtta
     5821 gatgcagaga acgtacgagc agtagacatg cctttggata tggatccgaa aacagcattg
     5881 gaccgaattg aacgcaatgt acaagaagta gatgaaggaa gcggggtcat actcttagta
     5941 gatatggggt cccttgcgtc gtttaattcg caaattcaaa gagacactgg aattccagta
     6001 cgtactgttg atatggtaac aacttctcta gttttggaaa ctgtacgaaa ggtctcagta
     6061 ttagggacag atctagatat gttgcatgaa tcattgaaaa attttcgagg ttacgcagaa
     6121 atatcttctg tagaatctgt tcaggaagca aaacctattc ataagaaaaa agctgttttg
     6181 gctatctgcg catctggcga aggtacagca caaagaatca aagaattgat cgaacgagcg
     6241 gtaaacaagc gtcaagaaac agaattagaa gttctagctt tgtcgattgt ggaagtgaaa
     6301 gaagaactgc cagttatcca aaaagaatat cagatcatag ctactactgg gattactgat
     6361 ttgaatatcc aagccccatt tattccttta gaacgtttta ttgatcaaaa tatcgaagtc
     6421 attttggatc agttgctgat ggaatcagaa ttggaggaaa ctgaattcat ctccctagat
     6481 gaagagtcag ctaaaaatac gtgtgttgag tttatctctg ataacttcat ctttattaat
     6541 ggtagtaagt taatcgatcc aatgtggcaa ttttctacac aaatctctca gacgacggga
     6601 atcggcgatg aagaatatgg attcaaaatc aacctagtga tgcacacagc aggaatgatc
     6661 gaacgtatta ttcggaatga accattgact gtggaagaaa acgaattgac aaatacaacg
     6721 aatgatcctc tttatagcca attggcagcc tcagttgttc tgttggaaga tcagataaaa
     6781 gtaaaagtgc cgatagaaga aatgtattat ctgctacgtc tagtccataa tcagttagat
     6841 aaaaaagaat acactgtgcc atgaatacac aaaaacgata cacttttata gtgtatcgtt
     6901 tttgtgtatt ggttaagaat cctttaaatt caattagtgt atcgtaaaaa agttggcacg
     6961 ctttttgctt atagtagtgt gtgctaggag gtggttgttc acacagaaat gataggggtt
     7021 tctgtgaagt agtgaggaag aaggagtttt gcagatggat attcgattgg cacgtataga
     7081 tgatcgttta atccacgggc aagtcgcaac agtttggtcg aagcaaacag gtgttgaacg
     7141 tatcattgtg atcagcgatg tagtcgctca agataaattg agaaagtttc tactaaaaga
     7201 agctgcaccg ccggggatca cagcaaatgt aataaccgtg cagaaaatga ttcaaatcta
     7261 tcatagtgga ttattatcac gagcaaaagt gatgctttta ttcaccaatc cccaagatgt
     7321 tgaagcggtt gtccgctgcg gtgtacagtt gaagtcgttg aatatcggcg gtatgagttt
     7381 tactgaaggc aaatcaatga ttaccaattt tgtttcagta aatcaacaag atattgattc
     7441 ctttcgttac ttgaacagtc aagaagtgga gttagagatt cgaaaagtac ctgctgacag
     7501 gaaaatcctt ttgatggaac tactagcgaa aatcaaaaag gtgtagcaag aatggttgtt
     7561 ggaaaaggtc aaataattct gttgtaatgg ttagcaatta ttaatatttt tgaatttgtt
     7621 acaacaaaag taaataggag gtataaatat ggtagggatt atcctagcaa gtcatggaca
     7681 atttgctgaa ggaatcttac aatccggttc aatgatcttt ggcgaacaag aaaacgtcaa
     7741 agccgtgatt ttgaaaccaa gtgaaggtcc agatgacttg agagccaaat tggaagaagc
     7801 ggttgcttct tttgataatc aagacgaagt gttattctta gttgatttat ggggcggaac
     7861 accgttcaat caatcaaaca cattattcga agagcacaaa gataaatggg caatcgtcag
     7921 cggtttgaat ttaccaatgt tgattgaagc ctacgcatca cgcttttcaa tggaatctgc
     7981 tcatgaaatt gcagcacaca tcattgaaac agcaaaagac ggtgtaaaag taaaaccgga
     8041 agaattagaa ccggcagaag caccgaaagc agcagttgaa gacgcacaac ctaaaggagc
     8101 acttccagaa ggaactgtgg tcggagacgg aaaaatcaaa tatgtattag cacgtgttga
     8161 ctcacgtctg ttacatggac aagttgcgac agcttggaca aaagcagtgc aaccaaaccg
     8221 tattatcgtt gtttcagatg cagtatcaaa ggatgatctg cgtaagagac taatcgaaca
     8281 agctgctcct ccaggtgtaa aagcaaatgt tattcctatt agcaaaatga tcgaagtagc
     8341 aaaagaccct cgttttggaa ataccaaagc attgctttta tttgaaaatc ctgaagacgt
     8401 attgacagct gttgaaggtg gcgtagacat caaagaatta aatgttgggt caatggcgca
     8461 ttcggtcggt aaagtcgttg taagtaaagt attgtctatg ggacaagaag acgttgaagc
     8521 gtttgagaag cttgaacaaa aaggtgtgaa attcgatgta cgtaaagtac ctaatgattc
     8581 acgcgataac atggatgata ttttgaaaaa agcaaaagct gaattagcaa aagcgtaata
     8641 aataatatta aaatttatag gaggcttatc atgtctatta tttcaataat tttagtcgta
     8701 ttagttgcct ttctagctgg tatggaaggt attcttgatg aatttcaatt tcatcaacca
     8761 ctagtggcat gtacattgat cggtttagtt accggtaacc tcgaagctgg tatcgtactt
     8821 ggcggtacgc ttcagatgat cgctttaggt tgggcaaata tcggtgccgc tgtagcacca
     8881 gatgctgcat tagcatcagt agcatcagca attattttag ttttaggtgg acaaggtgtt
     8941 aaaggtgttc cttctgcgat cgcgattgcc gttcctttgg ctgttgcagg gttgttctta
     9001 acaatgatcg tacgtacaat cgctgttcca atcgttcatt tgatggatgc ggctgctgaa
     9061 gaaggaaaca tccgtaaggt tgaaatgtgg cacatcattg ctgtatgttt acaaggggtt
     9121 cgtatcgcgg tcccagcagc agcgttgcta tttatcccag cacaaactgt gcaatcattc
     9181 cttgaatcaa tgccagcatg gttaacagat ggtatggcta tcggtggggg aatggtcgta
     9241 gctgtaggtt acgctttggt aatcaacatg atggctacaa aagaagtatg gccattcttc
     9301 gttattggtt tcgttgtagc tgcaatttct caattaacat tgattgctct aggtgcttta
     9361 ggtgttgcat tagctcttat ttacttgaac ctttctaaaa tgggtggttc atcaaataat
     9421 ggaggcggaa gcaactctgg tgacccttta ggcgacattt tgaatgacta ttaatcttga
     9481 aggaggaaaa taaaatggca gaagaaaaaa tcaaattatc aaaaaaagat cgtttagctg
     9541 ttgcgtggcg ctctaccttc attcaaggtt catggaacta cgaacgtatg caaaacggtg
     9601 gctgggcatt ttctatgatt ccagcaatca aaaaattata caaaacaaaa gaagaacgtt
     9661 ctgcagcatt aaaacgtcac ttggaattct ttaatacaca cccatacatt gcttctccaa
     9721 ttttaggggt tacattggct ctggaagaag aacgtgcgaa tggcgcgcct gttgatgatg
     9781 tagcgattca aggggttaaa gtaggtatga tgggtccttt agctggtgtt ggtgacccag
     9841 tgttctggtt tacagtacgt ccaatgctag gtgcactagg tgcttcatta gctatgggag
     9901 gaaatatcct tggaccaatc atcttcttct taggttggaa cattattcgt tgggcattca
     9961 tgtggtatac acaagaattt ggctacaaag ctggttctaa aattactgat gacctttctg
    10021 gcggattgtt acaagacgta acaaaaggtg cttcaatttt agggatgttc gtattagctg
    10081 cccttgtaca aagatgggta tcaatcaaat tcttaccagt tgtatcaact gtaaaattag
    10141 ataaaggtgc ttacatcgaa tgggataaat tgccttcagg tggtgaagga atcaagagtg
    10201 catttgaaca agttaataat ggcttagccc tttctcctga aaaggtaaca acattacaag
    10261 acaacttaga ccaattgatt cctggtttag ctgcattagg attaacattg ttatgtatgt
    10321 ggttgcttaa gaaaaaggtt tctccaatcg ttatcatcct tggtttattc gtagtcggtg
    10381 ttgttggaca cgtcatcgga cttttataat ccataaaaat atagctcggg cgttgtcccg
    10441 agcttttttt atatagtata ctgttcataa acaaatatct ggagggagaa aatggttcaa
    10501 tcaatcaata caaaagtaga cttggtaatt gatgctactt cgtttacggg tctgacagat
    10561 tacgggcaaa tcatgatagg tgataatggt tttgagtttt accatgcgcg agatagacgc
    10621 aaatatatcc aaattccatg ggaagaagtg gattatgtac tagcatcagt catgctaaag
    10681 ggaaaatgga ttcctcgata tgccattcaa acgaaaaaaa atggtacata cacattttct
    10741 tccaaagaac ctaaaaaagt gcttcgtgcg atcagagaat atgtagatga agatagaatg
    10801 gtacaatcat tgagtttctt tgatgtagtg aaacgtggta ttcgtagtat gtttaaacgc
    10861 aagtaaaaaa taaagaaaac ttctaagagg aggttttctt tattttttta ctttgcagaa
    10921 gaaaatatga aatagcaaaa ccaattattt gtccccttta aatcccatat gctaaaattg
    10981 attagcaaag aaggaggtta tattatgagt tggttatgga cattgattgt tggagccgtg
    11041 atcggagcaa ttgcaggtgc gatcaccagt aaaggaaatt cgatgggctg tgtaacgaat
    11101 atcattgctg gactagtcgg ttcaagtatt ggacaagcga ttttcggcga atggggacca
    11161 aaagctgcag gtatggcact tattccatct attttaggcg cagtgatttt ggtagcagtt
    11221 gtctcatttt tctttggaaa acgatcataa gtttttacaa aatagtaaac taaaaggagt
    11281 tgc
//
LOCUS       DAIGOK010000090        11249 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.090, whole genome shotgun sequence.
ACCESSION   DAIGOK010000090 DAIGOK010000000
VERSION     DAIGOK010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11249
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.090"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(13..1242)
                     /gene="hflX"
                     /locus_tag="NOA01_001965"
     CDS             complement(13..1242)
                     /gene="hflX"
                     /locus_tag="NOA01_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCI0633149.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1244..2161)
                     /gene="miaA"
                     /locus_tag="NOA01_001966"
     CDS             complement(1244..2161)
                     /gene="miaA"
                     /locus_tag="NOA01_001966"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCI0633150.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2165..2902)
                     /locus_tag="NOA01_001967"
     CDS             complement(2165..2902)
                     /locus_tag="NOA01_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCI0633151.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2993..3520)
                     /locus_tag="NOA01_001968"
     CDS             complement(2993..3520)
                     /locus_tag="NOA01_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633152.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3700..4293
                     /gene="clpP"
                     /locus_tag="NOA01_001969"
     CDS             3700..4293
                     /gene="clpP"
                     /locus_tag="NOA01_001969"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCI0633153.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4397..4882)
                     /locus_tag="NOA01_001970"
     CDS             complement(4397..4882)
                     /locus_tag="NOA01_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCI0633154.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5035..5232)
                     /locus_tag="NOA01_001971"
     CDS             complement(5035..5232)
                     /locus_tag="NOA01_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633155.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5327..7087)
                     /gene="efrB"
                     /locus_tag="NOA01_001972"
     CDS             complement(5327..7087)
                     /gene="efrB"
                     /locus_tag="NOA01_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HCI0633156.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7084..8814)
                     /gene="efrA"
                     /locus_tag="NOA01_001973"
     CDS             complement(7084..8814)
                     /gene="efrA"
                     /locus_tag="NOA01_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HCI0633157.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9207..9419
                     /locus_tag="NOA01_001974"
     CDS             9207..9419
                     /locus_tag="NOA01_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HCI0633158.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9421..11100
                     /locus_tag="NOA01_001975"
     CDS             9421..11100
                     /locus_tag="NOA01_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCI0633159.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         3263 a         2429 c         1971 g         3586 t
ORIGIN      
        1 aaaaataaaa ttttattggt tttttcttaa ccattttgaa ttggtttttg caaacccttt
       61 aacttcatat tcgtttgttt cttccatgta cgcttcgtcc aaaaccattg tttgtcttct
      121 caactcgctt aatttctgtc cttcgttgct tggcacagaa agtgtataag gggtcattag
      181 ttccatcagc tgacgtttga ttgcttcgat caacgccatc ttaccttctt tcgtctgagt
      241 agaaatcaat acatttggga atagcgtagg cgtgaacatc gctgggtcga tctggtctgc
      301 cttattgtaa acagtcagta tcggcatttc tttcatattc agttcatcca tcaattttaa
      361 gacagtctgc tcctgtaaaa tacgatctgg agaactagca tcgactacat gtagcaaaag
      421 atccatattt tgactttctt ctaatgtaga atggaaagca tcgatcagtt gcgtaggcag
      481 gtcttggatg aacccgacgg tatccgttac agtaatctca aatccttcag caaaacgcca
      541 tctcttagtc aatggatcta acgttgcaaa caattgatct ttcgaataag tatccgcttg
      601 tgtcaataga ttcaagatag ttgacttccc ggcattcgtg tagccaataa gtccaatctg
      661 aaaaatctcg ctattttttc ttttttggcg attgcgctct cgatgagctt ctacttcttt
      721 caattctttc ttaacagcaa aaattttatt ccgtatatgt cggcggtctg tttctaactt
      781 tgtttcacct ggccctcttg ttccgatccc accgccaaga cgagataatg ttttcccttg
      841 ccctactaaa cgaggcagca ggtattctaa ctgagcaagt tcaacctgca gcttcccttc
      901 tttcgaacga gcacgcatcg caaaaatatc taagataagc tgcacgcgat caattaccgg
      961 aagaccaaca gcatcggtaa tcagttggct ctgtctcgga gttagctcat gattaaagat
     1021 gatcaaatcc gcttcatgcg catccgcttg ttgaactaat tcttgtaatt tccctttacc
     1081 gataatcgtt tgccgatcca cttgtggtct cttttgtgta attgtgaata cgacttctcc
     1141 tgatgcggta ttcgtcagct gaactagctc ttccattgac tctgaaaagt aacgctggtt
     1201 tgcttcggtt tctaccccta cgatgatcac tttttcttcc attttattcc gcctccagcc
     1261 attttttgat tgtttgctca atctgacttt tttgatttgg ctcacttata agatcaaacc
     1321 actgtgcatc caaacggtta cgaaaccacg tcaactgacg tttcgcgtat cttctcgaat
     1381 gtagtttgac ttgttctgtt gcttcttcca atgtttcttt cccttcaaaa taagggaaaa
     1441 attctttata tccaatacct tgactggcct ggacttctcc aagctcatac accttacgcg
     1501 cttctgctac taaaccttct gctagcatca aatcgactct ttgattgatt cgttcataaa
     1561 gaatactacg cttcgtatct aatccaatca tgaggtaatc atacaattgc tttggctctt
     1621 ctttaggtgc cgtgatacta tgtcctgtcg tctctaaaac ttccagagct cttatcactt
     1681 tgcgttgatt gttccagtgg attttctcag cagctgccgg gtctttttct ttcaactgat
     1741 tccataatgc ttgggaacca attgtttcag ctaacgtttc atactttttt ctaatctcag
     1801 gattcgcttc ttctgtcgct cctaactgat aatcatatag aagggattgg atatagagac
     1861 ccgtaccacc agctatgata ggtaggtgcc ctcggttcgc aatctcggta atcaattgtc
     1921 gacctgaatg ctgaaaatca gcggctgagt aacgttgagt aggttcgatc acatcaatca
     1981 aatgatgcgg aacattattc atctcttctt ctttgatttt tgctgtaccg atatccagat
     2041 ttttatagat ttgcaatgaa tcaccactga tgatctctcc tccaaatagt tgtgcgagtt
     2101 ccacacttag cgctgtttta ccgactgctg tcggaccgac gatcactaat actttttcca
     2161 tattttacct acttttcgtt gattgtttcc accgaagagc tttctccgga aaatcagtta
     2221 ttattccatc tattttgagt tcttgacatt tggccatttc ttcttcatcg ttaatcgtcc
     2281 atgcacgaat tgcttttgga aaatcagctg cctggactgg atgttcgaac aaccaagtat
     2341 tttttggatg tactccttct atataagggg tttctgctgc tagttggatc tttttatcag
     2401 aagtactcat caataagtcc agctgtatct ccggttccag taatgcgatt ttttccaaag
     2461 aatccaaatg aaagctgcaa tatgcatgtt gaaaaggcca atctctactt tttaatgttt
     2521 cggctaattt ttcttcgata ccgggatatt catactgatc agttttaatt tcaatcatta
     2581 gcatccctcg gtaatttttt tggaggagca gtcctaacac ttctctcaat tcaggaactt
     2641 tagctgctgg aaattctttg ctgaatttgc cccctgcatt caattttttg agttcttcca
     2701 atgttttttc tcgaatcagc cctttaccgt tagtcgtccg gtccacttct tcgtcgtgca
     2761 taacgatcaa ataaccatct ttgcttagat ggacatctag ttcgatacca tctgcaccta
     2821 ctcgtacagc ttctgcaaat gcaggaagcg tattttctgg gtgggtgccg ctgcttccgc
     2881 gatgggcata gatttgtaac atctttcttc actccgttca gttctattgt atcattaaga
     2941 aaaaaagaaa gcattaccac gctctcatgt gcaacggcaa tgcttcaaaa tatcagctcc
     3001 aaacatcatc tttcgagaag aaaccaatcg gctggcgcgt catagtcttc aactgagctt
     3061 ttttttcatt gatcatttca gtcagactat ccgtaaaatt ctctaaaatc acgcgtccgc
     3121 ctttataagc atatccacca atcttcattg tttcatctgg agataaaatc acttttcgat
     3181 gggagatatt cttaaatagc tcgtctaaaa tataatttgg cagataaaaa ctattttgcg
     3241 ctgaaaatga ttggagatac gcaaaatcgt caatcgtgat ataacaatca tcaaatgcat
     3301 caaagctgac atattcttcc atcagcgttg tccgtttttg caagatctcg ttgatctttt
     3361 tatttaattg attcagcgtc tcgatttctt tgactttcaa ttccaattca cgtctacgct
     3421 gttctttatt ttccaagtga cgcatttgca gtatcatgcc gatatagaca actacagcac
     3481 ctgtcagcac tccgataagg ccagtaatac caactatcaa tctgttgttc ctccttgttt
     3541 tgtttctctg cttatcatac cataaagcgg gtttatgcga ctaatttgcc taagtttttt
     3601 atttgacctt ttttgaccaa aaatgtatac tacattttag gtattagaaa ctaaattatg
     3661 gtacactatt tctaaacgaa atcaaggagg aactctatta tgaatttaat tcctacagtc
     3721 attgaacaat cttcacgagg tgaaagagct tacgatatct actcacgttt attgaaagac
     3781 cgtatcatca tgttaagcgg ccaagtaaca gatgatcttg ctaattcgat catcgcccaa
     3841 ttgcttttct tagacgctca agattctgaa aaagatatct atctttatat caattctcct
     3901 ggtggttcag ttacagcagg tatggctatt tacgacacaa tgaactttgt caaagcagat
     3961 gtccaaacaa ttgttatggg tatggctgct tcaatgggaa gcttcttgct gacagctggt
     4021 acaaaaggta aacgatttgc tttaccaaat gctgagatca tgatccatca acctcttggc
     4081 ggcgctcaag ggcaagcaac tgaaatcgaa attgcggcac gccatatttt gcaaacacgc
     4141 gaacgcttaa acaaaatttt agctgaacgc actggacaac cgcttgaagt gattgaaaaa
     4201 gatacagatc gtgacaacta tatgacagct gaacaagcaa aagcatatgg attgatcgat
     4261 gaagtcatgg aaaacagttc ttcattaaat taatctgttt caaaggaagc ttttttaagc
     4321 tctcttatct aaaccaaata aaaaaggatg gtcaactcaa actgaccatc cttttttatt
     4381 ttatggatgc tactgcctat ctttgctcct tgaattttcc ttgcgcatgc atccgaccta
     4441 tctcgacata atgctggatg ttttctttgt ttttttgtat ttcttctttt gtcaaaggac
     4501 ggatcacttt agctgggcga ccaaaagcca agacattagg aggaataact tttccttctg
     4561 ttactaatga tccagcacca atcaaactat tctcaccaat aaccgcatga ttcaatatcg
     4621 tggtccccat cccgatcagc gccccttttt cgattgtaca gccatgtagc atgcactgat
     4681 gtcctaccgt cacattctcc gcaatatcta caggtgcatc atgatcgaca tggatgattg
     4741 taccatcttg gatattcgtt ctttgtccta tttttatcca attactgtcc ccgcgtaaaa
     4801 cagcttgata ccagatcgtc acatcttctg acaatgtcac atcaccaatc actgtggcat
     4861 ttgatgctat aaaccgttcc atatttattt ttctcctttc attttttgta gaaactaatc
     4921 ctattgaata aaggtatcat agcatatatt tatttcataa gaaaaaaaac aggtaaaacc
     4981 tgctcaggcc agatgtctat ttacagtatc tggtaactga cgttttacct gtttttattg
     5041 tacatctata ttttcttcta ttactttttt taaaattaat tcgtacactt ctatttgtgc
     5101 tgccaagcgt tcgatttgaa actcattttc aaaaaaatca gctgaatcaa aaggcttcgt
     5161 ctgacagacg tgcgttaaac gatcaacttc tcttttggct tcagtaattt tttcatttaa
     5221 aaactgttcc atctccgtgt cctcactttt cttgataact ttactttacc attatcaaga
     5281 atagagaaca aaaaataagt tttaaactgt tttattctta tatgacttat tctgcttcct
     5341 cagcaaattg gctgttatac agtttttcat aaaatccacc ttgtgccaat aaactctcat
     5401 gcgttccttt ttcaatgatc tgaccttgat ccatcactag aatcagatct gcatcacgga
     5461 ttgtcgataa acggtgagcg atgacgaaac ttgtccgtcc ctccatcacg cgatccattg
     5521 ctttttgaat caacgcttct aaacgggtgt ctactgaact tgttgcttca tctaaaatca
     5581 agattttagg atcggagacc actgctcggg caatcgtcag caactgtttt tgtcctaaag
     5641 acacattgtc tccctcagaa ttgatggcca tatcatatcc atctggcatc gttcggataa
     5701 aatgatctac gtttgctgtt ttcgcagcat caactacttc ataatctgtc gcatcaagct
     5761 ttccgaagcg gatattatct gcaatcgttc cttgataaag ccatgcatct tgcaagacca
     5821 taccaaatag cgaacgcaca tcactgcgac tcattgattt ggtgtcgatt ccatcaatcc
     5881 ttattgctcc tttgtctacg tcataaaaac gcatcaatag attgatcaat gtcgtcttgc
     5941 cggcaccggt cggaccgacc acagctacgg tttgaccagc tttcacttca aaattcaaat
     6001 cttggattaa tggtttcttc ggatcatatg caaaatcgac atgatcgaat gtcacatctc
     6061 ctaaaatcgt ttctggcagt gaagcatcta ctttgttttc tttttcttct ggctcatcta
     6121 aaatctcgaa tacacgtttc gttgccgctg atgcactttg caaaactgaa gacaattgtg
     6181 tcacgttccc cattggctga ctgatttgcc aaatatattg gatgaatgct tgtagttgac
     6241 cgacaacgat cgccccagta ataacataaa agcttcctag aacagccata ccaatataag
     6301 tagcataggc agttaattga actagaggca tcatcaagcc agaaatgaat gctgcacgaa
     6361 acccaaagta tttcaagctg tgattaactt ttttgaatcc ttccaatgtt tctttttcgc
     6421 gtccatatac tttcaataca ctaaaacctg tcatattttc ttggacgtag ccgtttagct
     6481 caccaagaga attctgcatg ctttgaaaat atttctgcga agcattgatc actcgttttg
     6541 agatccagat cgatgttgga atcatgatca tagagatcag cgccatcatc ggctggatat
     6601 aaaacatcat tgctgcagcc aacgtaattc ccaatatcgc atttacgata ccaataaaag
     6661 cttgttgtaa agcaccgctg actgcatcta catcattcgt cacacgagac aaaatatttc
     6721 cttgctggtt tttatcgaaa tatgaaaccg gaagacgatt gattttttct tctatgtctc
     6781 ttctcaaatc atgcatggca ctttgaacga catttgtcat caaaacacca gaaagtaatt
     6841 gcgaaataca ataccctatt ccaacaactg tgatccaaat gagacatttg aatatataag
     6901 aaaaattgat tgattcgcca ttggcaatat tacggctgat ttctgtagta ggaagtcctg
     6961 taacatatgg caaagccacg ttgaaagcca ctgtcagaac tgtaaatagg atcactaaaa
     7021 gaaaagtctc acgatacggt ctgatatatc gagctaaacg ttttaaggaa gaaaaggttt
     7081 ttttcatgct aattcctcct ttgataattg agaagcggca atatcataat aaatcgggca
     7141 tttttccaac aactcccgat gcgtccccat accgacgact tctccttcat ttaatacgat
     7201 gatcttatct gcatgcatga tcgttcccac acgttgtgct acgatcaata cagtagattc
     7261 tgtcgtttct tttttcagac gagcacgtaa cttcgcatcc gtttgataat ctaatgcaga
     7321 aaagctgtca tcaaaaatat agatttccgg acggcgtatg atggctctgg ctattgccaa
     7381 tcgttgtttt tgtccacctg aaaaattgga accaccttct gataaatgtt cattaaatcc
     7441 atctggtttt cgagaaatga attctgaagc ttgagcaatc tcagctgcac gttccatttc
     7501 ttcttgcgta gcatcttctt tcccgtatcg caaattatct gcaatcgttc cagtaaatag
     7561 cagcgctttt tgcggaataa acccaatctt atctcgcaac ttgcttagcc gataatctct
     7621 cacgtctact ccatcgatca agatctttcc ttcactcaca tcataaaagc gagggatcaa
     7681 ttggatcaac gtagattttc cgctaccggt acttccaata aaagcaacgg tctctcctgg
     7741 agaggctgta aaactgacat ttcgtatcac tggctcttgc gaatggcctg gataagcaaa
     7801 tgttacattt tgaaattcga tatacccttt agtttttgta tcagatatac catttggatc
     7861 ttctacaatc gaaggtttgg catctagggc ttcttggata cgtccagctg aaacagctgc
     7921 tcttggatac atcatgaaca cactagcaaa taacatgaat gagaacaagg catggaatat
     7981 atactcgata aacgcaatca aatctcctac acgaagctcg cctgcatcga tttgcactgc
     8041 cccgctccag atgatcaata ccattacgat attaaataag aaataaaaac ccggttgtgc
     8101 aacagccatc aaacggaata agctttttga actagaagca taattctcat tgactacagc
     8161 aaaacgtttt tcttcaaact tttcattgac gaatgcacga atcacacgca atcctgaaag
     8221 attttcccgc aagatactgt tgattttatc taagtttttt tgttgctttt tagataacgg
     8281 ttcagacacc ttagcaatcg ctacgactgc caataatagt aaaggtaatg cacctactac
     8341 atacaaactg agtgtcacac ttgtacgcat gaccatgtac aaactgacaa tgaacatcat
     8401 tggtgccata aaaccgatac gtaaaatatt ttgcaggaac aacatgatct gataagcatc
     8461 gtttgttgta cgagtgatca atgaagatac gcctagattt tcgtattcct gatgcgaata
     8521 actttggatc ttttcgaaca aatcatctcg aatatctgct acgatattag tcgtaatcct
     8581 cgaaccaaaa taacctaaca agatattcaa aataataccg attacagtaa taacgatcat
     8641 aaatatccct tgttgcttca cataatcaaa atcatcattg cggattccta catcgatcat
     8701 acgtgctaaa atcgtaggaa gtcctaattc aatcaaaata aaaccaaata cgcatatgaa
     8761 attgaaaaac aagaatttct tataacgcat ggtataatgc cacattagtt tcactttctc
     8821 atctcctttt aatcttttgt tacattttca ttactaaaaa tgatagcaca acgagcctat
     8881 taatagaaca cttctctcat aaaaaagtca ttatgattat agcgggatga taaacattga
     8941 ttttttcttt caatctagtt gatttctata cttatttgct gttttatgct agaataacaa
     9001 ttaattgttg tatatttttt atacagaagg tacggttgaa aaaatatccg aacagataag
     9061 caaaaacgag gcttgtctct ttgactcgaa tccagtattc tagtcaaagc atatgaaaaa
     9121 ccaggtggct tgtcccctcg ttttgtgttg tctaaaaaaa tcaactagct aaatcactgc
     9181 tttacgcaga taaaggagac aaaacaatga tctataaagt ttattatcaa gaaacaaaag
     9241 tacgcaaccc taaacgggaa gaaacaaaat ctctttacat tgaggcaaaa agcgatgtag
     9301 acgctcgcca acgtgtggaa gaaaataccc catacaatat tgaattcgtt caacttctag
     9361 aaggaagtca tctagagtac gaaaaagaaa atgctgattt ctcattagtg gagttttaat
     9421 atgaaacttt ccatcaaaaa taacgagaca ggcgtctttg ctattggtgg attaggtgaa
     9481 atcgggaaaa atacgtatgg ggtacaattc caagatgaaa tcattttgat tgatgccggg
     9541 atcaaattcc cggaagacga tttacttgga attgactatg tcattcccga ctacagctac
     9601 atcatacaaa acttacacaa agtaaaagcc ttggtgatta ctcacgggca cgaagaccac
     9661 atcggcggaa taccctactt gctgaaacaa gcaaatattc ctatctatgc tggtcctctc
     9721 gcattagcgt tgatcacaaa caaattagat gagcatggct tgcttcgaga tgcagagctc
     9781 catgagatca atgaagatac gatcatccgt tttagaaaga cttctgtcag ctttttcaga
     9841 acgacccata gtatcccaga tgcattaggg atcgtggtga aaacaccttc tggaaatatc
     9901 gtagcgactg gagacttcaa attcgatttc actcctgttg gcgaaccagc aaacttgcat
     9961 aaaatggctc gtatcgggga agaaggagtc ttgtgccttt tatctgatag tacaaatgct
    10021 gagattccaa catttactaa atcagaaaaa acgattgggc attctatttt gaagatcttt
    10081 tcaaaaatcg aaggacgtat catttttgcc agctttgctt ctaacatctt ccgtttgcag
    10141 caagcagcag atgccgctgt ggcaactggt cgtaaaatcg ctgttttcgg acgaagcatg
    10201 gaaaatgcca tcgtcaacgg tcaacgttta ggatatatca aagttccaga cggcacattt
    10261 gtagatgcac acgaaatcaa tcgcttgcct gccaatgaaa cgatgatcct atgtacaggt
    10321 tctcaaggag aagcgatggc cgcgttgagc cgcatcgcga atggtactca ccgtcaaatc
    10381 caaatccagc cagacgatac agtgatcttt tcaagttcac ctattcctgg taatacaaca
    10441 agtgtcaatc atttgatcaa tttgctgtct gaagctggtg cagaagtgat tcacggaaaa
    10501 atcaataaca tccatacttc cggtcacggt ggacaagaag aacaaaaatt aatgcttcgt
    10561 ttgatgaagc caaaatattt tattcctgtc cacggagaat tccgcatgtt gaaagtacat
    10621 gcttctctag cacaagatgt aggtgttcct gcagaaaact gcttcatcat ggaaaatggc
    10681 gatgttttag cgttaactag cgattctgcg cgtcctgctg ggcattttgt ggctggagat
    10741 gtgtatatcg atggtagcgg gatcggtgat attggtaatg tcgttttacg tgaccgtcac
    10801 ctgctttcag aagaaggtct tgtattagct gtcgcgacag tggatatcaa gaaaaaagaa
    10861 atcttggctg gtccagatat tctctctcgt ggatttgtct acatgcgaga atcaggagaa
    10921 atgatcaatg aagctcagcg aatcttattc cacgctttaa gagaatgttt gaatggacct
    10981 gactgttcag aatataaatt gaaagaagcg atgactggcg cgcttcagcc tttcttattc
    11041 gaacgtacag aaagacatcc aatgattttg ccaatggtaa tgacaccaga tgaagaataa
    11101 ataagaggtt atgaaatcag caccttgtcc tgaatataag agaaaacaaa tgaaaaacat
    11161 gagaagccat ttttgtttgt tcttattcct tgatacaagg tggctctttc atagcctctt
    11221 aaatttattt actttttcat acaaaaaaa
//
LOCUS       DAIGOK010000091        10936 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.091, whole genome shotgun sequence.
ACCESSION   DAIGOK010000091 DAIGOK010000000
VERSION     DAIGOK010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.091"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..520
                     /locus_tag="NOA01_001976"
     CDS             <1..520
                     /locus_tag="NOA01_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCI0633160.1"
                     /translation="FLEILEDHSAAIFEKPQIQQLTNIVSPAVRGVFLKFLEKAESEK
                     IPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITINLLEEKEGSRFSI
                     ANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELKIEFSKQNHYFFVE
                     MYLASRKSKSAY"
     gene            842..1069
                     /locus_tag="NOA01_001977"
     CDS             842..1069
                     /locus_tag="NOA01_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCI0633161.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            1192..1518
                     /locus_tag="NOA01_001978"
     CDS             1192..1518
                     /locus_tag="NOA01_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCI0633162.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            1586..2299
                     /locus_tag="NOA01_001979"
     CDS             1586..2299
                     /locus_tag="NOA01_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HCI0633163.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            2609..3460
                     /locus_tag="NOA01_001980"
     CDS             2609..3460
                     /locus_tag="NOA01_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCI0633164.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            3581..5446
                     /locus_tag="NOA01_001981"
     CDS             3581..5446
                     /locus_tag="NOA01_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCI0633165.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            5494..6903
                     /locus_tag="NOA01_001982"
     CDS             5494..6903
                     /locus_tag="NOA01_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HCI0633166.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            7011..7712
                     /locus_tag="NOA01_001983"
     CDS             7011..7712
                     /locus_tag="NOA01_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCI0633167.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            7871..8350
                     /locus_tag="NOA01_001984"
                     /pseudo
     CDS             7871..8350
                     /locus_tag="NOA01_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            9198..9773
                     /locus_tag="NOA01_001985"
     CDS             9198..9773
                     /locus_tag="NOA01_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HCI0633168.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            9773..10213
                     /locus_tag="NOA01_001986"
     CDS             9773..10213
                     /locus_tag="NOA01_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HCI0633169.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            10229..10930
                     /locus_tag="NOA01_001987"
     CDS             10229..10930
                     /locus_tag="NOA01_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCI0633170.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT         3701 a         1781 c         2384 g         3070 t
ORIGIN      
        1 atttttagaa atattagaag atcattctgc agctattttt gaaaaaccgc aaatacaaca
       61 gctaacaaat attgtatccc cagctgtcag gggtgtcttt ttaaaatttc tagaaaaagc
      121 agaatcggag aaaatcccga tccaagtagt tattcctgtc gaagtgaaaa caatcccgat
      181 tgatctggtt gtttttattc gctgtttatc tatttttatt tctaacgcat tcgatcatcg
      241 tgtagccgat cagagtccga ttacgatcaa tctgctggaa gaaaaagagg gatcacgttt
      301 tagtatagcg aacaaggcaa atccaacgaa tttgacgcta tcggagatgg tcaaaagagg
      361 aaaaacctct aaaaaaaatg gcgggttagg tctatacagt gcgaaaaaaa tgttggatgt
      421 ttacgaaaat gcagaactaa aaatcgaatt ttcaaaacag aatcactatt tttttgtaga
      481 aatgtatctg gcatctagaa agagcaaatc agcctactga cttactgtca aaggataagt
      541 ttgaggaaag tctacacgga gatagagaca acaacacagc atattcaaaa gaaacagaag
      601 cgtgatgacc gttgtcaccc ttctgtttct tttgctatgt agttaaacaa agtagttgac
      661 acttccgcta cttagtatta gtacgaaagt atacttcatg atactaagta gataagaagt
      721 gaaaagcgga acccacgatc gtcaaaatgc taactgcttt gcttacacca gataaaggga
      781 agagcaatat cgcttatttt aggcgtggag aagcaagcaa atccctacga tcacaaaaaa
      841 aatgctgacc cagcagttaa gagagttaga acaagatgaa agtatccatc gaaaagccta
      901 taaccaagtc ccgcctaaag tagagtactc gctcaccgaa gaaggaaaat cattaagaga
      961 gattctagta gcgttgtctg tttgggaaga agaacgtatc gaaaaacaaa aagcctcaag
     1021 ggaagaagac acgctactgc atacacatgg tggctaccta aattattgat tttctatgac
     1081 atttatttta aataaaagaa aacaggagtt tttttaaaag catcatctat tttttatgta
     1141 aaaaaatggt atgattaatc aagtaagaag tgattggaag aaggcaagca aatgaatagt
     1201 atggaaaaag tcaaccagtt tagagatgaa cgtaattggc gacaatttca taacgaaaaa
     1261 gatttggcta tctctatttc tttggaagca tctgaattgt tagaactttt tcaatggaaa
     1321 acgccggaag aagtaaaaga aacacaatta gaacgaatca aagaagaact ggcagatgta
     1381 ttgatctatt catacatgat ggcagacaac cttcacctgg atctagatga gatcattgaa
     1441 gaaaaattga tcaaaaataa tttgaaatat cctgtagaaa aaagcgcagg tgtacgaaaa
     1501 aaatacacag agttgtagaa atgtgtcatt cggctgttgg aaagagcaaa agaaaaacag
     1561 taaaatgagt ctggagggag agaaaatgaa aaaaaaagga aaagcaaaaa gatggctagt
     1621 caatatcctc ttgttcctct tgttattagt aggactagct ttgattttca atgaacagat
     1681 caaggattat tttgtcagag agacaggaga taaatatgct atagcgaatg taacaaaaga
     1741 agatctgaaa aagaataacg ataaagatgt cagttttgac tttgatgcgg tagaaccaat
     1801 gacgacagaa ggggtcatga ggtcgcaaat gagaggaaca gatctaccag tgattgctag
     1861 tatcgcagtc ccttcagttt caatcaattt accggttttc aaaggcttgg ataatacgtc
     1921 actactttac ggagcaggaa cattatctcc agatcaagaa atgggaaaag gaaattatgc
     1981 cttagcaagc catcgtgcaa cgaaccctga attgctgttt acaccgctag aaaatttaga
     2041 gatgggtgcc aagatctatt tgacagattt agaaaatgtg tatacataca aaacattttt
     2101 taaagaaaaa gttgctccta cagataccca attactgaat gaagtcgagg gaaaagaaat
     2161 tgtcacacta attacttgtg gcgacatgga cgccgtcacg cgactagttg tacaaggaga
     2221 actggaaagt gttacatcga taaaagatgc gacagatgat atgagatctg ctttcaatct
     2281 agagacaaaa acattttagg atactgggac ataagtcgtt acagattaaa aacagccaaa
     2341 ggatatgaat cgataaggaa aaaccaattt gctgaaaaaa agagaaacgc aaagaggtgc
     2401 atctctgatt tggttgtttt ctagatagac ttgtgttcca gtttttttat tttttatatg
     2461 aaaaggcttg cataaataaa aattcgatgg tatgatgatt ttgaataaga aatggattgt
     2521 tactgtacgg caggcaaaac cagaattcag ctaagaaaca gtaggcgaat tctaggtttt
     2581 tttatttttc ttgagagggc gaagcaaaat gaaaattgat aaaattttga ataacaatgt
     2641 cgtcatttcg aaaaatggat tcggggaaga agtcgtttgt atgggaaaag gactagcttt
     2701 tcaaaaaaag attggggacg agatttcgcc agaagcggtc caaaaagaat ttgttttaag
     2761 agattctttt gctaaaaacc aattccagca gctgatggct gatattccgg cagaggagat
     2821 cgaatgggtc aaacaagtag tcgaactggc tgaagataaa ctaaaagttg aattttcgcc
     2881 taatatttat ctgacattga ccgatcatct tcattatgcg atcacacgag caaaagaaaa
     2941 tatccaactg cctaatccat tattatttga aacaaaaaaa ttttatccga aagaatatca
     3001 agcagcgaaa gaggctcttt tgttcgtgaa acaaaagacg ggtgtggaac tttctgagga
     3061 tgaagccggt tttattgcct ttcattttgt gaatagccaa caaggaaacg aagacatgca
     3121 agtgacgatg accgctacca caatggtaag agatatactg agtatcatca gtaaattctt
     3181 cggcatcgtg ttcgatgaag aatctttgaa ttatcagcgt atcattactc atctgcagtt
     3241 ttttgcccag cgttatctca aaggggaagg atctgacgaa caagatgaat ttctctacgc
     3301 attagttcaa ggaaagtatc caaaagcgtt tcattgtgtc gaacggatca atgagtatct
     3361 tcgacaaacg caaaaacagg aaatggggat cgcagagcag atttatttaa cgattcatat
     3421 tcaaagagta gtaagtgaga aaaaaagcat ccaatcataa ggattgttac tgtatggcag
     3481 gcaaaacctg gattgaaaaa ataggttcac gaatgtttgt gactgttttt ttcaatctgg
     3541 gtttttattt tttaaataaa aaagaaagaa ggaaaaaaca atggataatc aagcagttgg
     3601 tcgtcgtgta tgggaagcgg taggaggaca aaagaatgtc aaaagtttag tacattgtgc
     3661 tacgcggttg cgattcagat taaaagatga atctttagca gatacgcaaa agttaaaaga
     3721 agacccggac gtgatccaag tcgtacaaag tggcggtcag tatcaagtag tgattggcag
     3781 taatgtagca gatgtttatc aatcgatcgt ggatgaggaa ggtcttgcag tttcggagga
     3841 tggaaaagaa gagtccaaaa atattttgaa tcgtctgatc gatattatct ctagtatatt
     3901 tacacctttt ctaggggcga tggcagccgc cggggtattg aaaggattct tatcattggc
     3961 aacggtgatg ggctggctga cagctgatag cggtacttat caaatattgt ttgctgcagc
     4021 agatggtgtc tttaccttct tacctgtcat gcttgctttt acagcagcta aaaagttcaa
     4081 agccaaccag tttttagcag tagcaattgc tatggcactt gtgtatccag ctatcacagc
     4141 agttgctggt gcgggagaag ctatcagttt ctttgggatc ccagtgattc tttcaccatc
     4201 tggttatact tcttctgtca taccaatcat tctagccgta tgggttcaaa gtaaattaga
     4261 gccattcgta aaaaaagtga ttccgcaatt tctgcaaatg attttagttc cgcttgtcgt
     4321 tttagtagtg atggttccat taacatttct tgctttagga ccaatcggga cggttgccgg
     4381 taatgcttta ggcggtctct tcaactcgat ttatggattt agtccaatcg tagcaggtct
     4441 aatcatggga agtttatggc aagtattcgt gatgttcggc atgcattggg gattcgtacc
     4501 gatcatgttt ttgaatatcg aacaatatgg gtttgatgta ttgatgccaa tgcttcttcc
     4561 agccatcttg gcacaaggtg gggctgcttt agctgtagca ttgcgaacaa aagatacgaa
     4621 acttcgagca ttagggattt cttctacagt gacttcgtta tttggaatca ctgaaccaac
     4681 tgtgtatggt gtgacattac ctttgaagaa accattcatt gccgcttgta tttctggagg
     4741 tattggtgga gctattatcg gattttccgg agtaaaagct ttctccagca gtttggtcag
     4801 cttattgacg attccgactt tcattaatac ggtggatggt gtagagtcaa atgtgacagt
     4861 agcagtaata gctacaggaa ttgcttttgt tcttgcattc gtaggtacat tgattttagg
     4921 atttgacgaa caaacacaag acaatcaatt agaaaacaaa catgcgaatg caggagaacc
     4981 gattacttca gcacggcata ccttaaaaag tcctttaacg ggtaaagtac tgccattgag
     5041 tgaagtacca gaccaagttt tttcttctgg cgtgatggga aaaggtatcg ctattgatcc
     5101 agaagtagga gaactagttg cacctgcaga cggagagatc acgactattt ttccaactgg
     5161 acatgctgta gggatcacaa cgactgacgg agcggagatt ttgattcata tcgggatgga
     5221 tacagtagaa ttaaatggga atggctttga gatcttagtc aaacaagggg atctcgtcaa
     5281 agcaggagat ttattgattc gttttgacat cgaagcaatt agagcagctg gctatagcgt
     5341 aatcactcca gttgtcatta cgaatactga tgcatttgcc gatatacttg aattagacca
     5401 aaaagagatt attgcaaacg aagatgttct ggcaattgta aaataaggag gaaatcagga
     5461 tgcaatcacg tttttcagaa aaatttttat ggggtggagc aactgctgcc aaatcaatta
     5521 gaaggggctt atgatcgaga cggaaaagga ctatccgttg cagatgctat gcctggaggt
     5581 aagcagcgct ttgcgattat cggcagtgaa gaatttgact ggacgattga tcaagaaaaa
     5641 tatatttacc cgaatcatcg aggaatcgat cattacgatc gtttcaaaga agatcttgcc
     5701 ttgtttgcgg aaatgggatt caaatgttat cgtttttcga ttgcctggac acgaattttt
     5761 ccaaatggag acgaaggtac tcctaacgaa gcgggattag aattttatga tcagctgatt
     5821 gatgagtgct taaaatatga tattgaacct gtgatcacga tttctcatta cgaaatgcct
     5881 cttcatttgg caaaagaata cggtggctgg aaaaatcgaa aactgatcga cttctatgaa
     5941 cgatttgctc agacagtatt agagagatac agcagtaaag tcaaatattg gatgactttc
     6001 aatgaaatca attcggcctt tcactttcca gctttaagtc aaggattagt aaaaagcaat
     6061 ggagcaggag agtaccaaaa tattttccaa gcatggcata atcaattcgt tgcaagtagc
     6121 aaagccgtaa aaatcggcca cgaattgcgt tcggatatcc aaatcggctg catgattatc
     6181 tatgcaacaa cttacagcat cgatgccaat ccagtcaatc aggcagcaac aatgatccaa
     6241 aaccaagaat tcaatttttt ctgcacggat gtccaagtac gtggagaata ccctgcatac
     6301 acagcgcgta cccataaaaa atatggagtc gatccggaaa aattagagca aacggaaaaa
     6361 gacttccagt tgctaaaaga atatccagtg gactacatcg gatttagtta ttatatgtct
     6421 acggcaatca atgagactga tccacaagca gcaacttcag aagggaactt gctgggagga
     6481 gtaaaaaatc cgtttcttga ggcaagtgaa tggggctggc agatcgatcc agaagggctg
     6541 agaatcgcat tgaatgaatt atacaatcgt taccagaaac cattattcat tgttgaaaac
     6601 ggtcttggag ccattgatca tatggaagag gatggcacga ttattgatga ttatcggatc
     6661 gattatttac gtcgtcatat cgaagcgatg gctaatgcag tagcagacgg tgtagatttg
     6721 atgggctata cgccttgggg atgtatcgat cttgtcagtg catcaacagg agaaatgagt
     6781 aaacgttacg gctttatcta tgtggatcta gacgatgaag gaaatgggac actgaatcga
     6841 tcaaagaaaa aatcttttga ttggtataaa gaagtaattg cctcaaatgg cgtaaacttg
     6901 taattaaagc agctgaatga aaaagaacac acttctcaca tgtgatttgt gttctttttt
     6961 tattttttat gtgctattct tttagtaata gtaaagcaaa gggtgacgtt atgaaaagga
     7021 cacgtgtact atacttggaa gtcgcagata aaattaaaga agatattttc tcaggaaaat
     7081 atccagtcgg ttcgatgttg ccgacagaaa gtgaattaga agaactgttt caagtcagca
     7141 aaatcactgt ccgaaaggca atcgaactgc tggcatcaga tgaatatgtc gcaaagaaaa
     7201 gtggtcgtgg gacgacggtt ctaagcaatc gtccttacaa tcgattatcc aaagcggcaa
     7261 gctttacgca aatcttggag aactcccgat atgaagtccg gaaagaaacg ctggatttgc
     7321 aattggtcca tataaagaag gaagatcctt tgttttcctt tttcggtgag caagctgttt
     7381 gttttaagcg cttatattat ttgaacaatc agccttatat ttattttgtt cattatcttc
     7441 cacaagaaat gatgcaattc agcagagaag aatttggcaa agaatctttg tatcgtttgc
     7501 tgacgaaaaa aggatatact attgactcat tttctgatga tttctcggca gtatttttaa
     7561 cagaagaaga acaaggaatt ctgaaaacag acgcacagat cggcatcaaa cggatacgga
     7621 aatcgtctac gcaaattggg gaaatgattg aatattcgga ggcggtctac catacggagc
     7681 tgcatcctta ccatattgat tatgaaacat aaataaaaaa agaggttgtg aaaaagctgc
     7741 cctgcatcaa gtaataagaa cagccaaaca aaaatagctt ctcatatttt tcgtttggtt
     7801 aggcttatta ccgcagatac acggctgttt ctggattacc atttattctg gataaggggg
     7861 tgtggcacga ttttcagaag gaatctatga accaatcact gtattgattt tagcttcgat
     7921 ggtaacccca ttcggcttga cgatcgctta tttcttaggg aaaatcattc gtaaaaatat
     7981 actcaatcgt caagaaatcg atacattgaa gaccgctttt cctatgggga tctgtcagat
     8041 cacagaggga tgtttcccta tcgtattgaa cgacttggca cgcaatgtca tagcaacggg
     8101 agtcggtggg acagtaggtg gcggattaag catgttttgg ggcgcagata gccgtattcc
     8161 ggcatcggga atgtttgcag tcgctacgat gaccagacca tgggcattca ttggtgcatt
     8221 gttagctggt tcttttgtca caggaataac cattttatta ttgaaaaaac cagtagatcc
     8281 gaatgcagaa atcatccaag aagaaaaaga ggaagaagac atatcttggg atgatttgac
     8341 aattagttag gagaggcaga atggattaca agaaaaaagt acaatagatg taccttgaga
     8401 caggaatggt atgaaagaat cagagttaga aagaatcgaa tatacagatt ttggtttagg
     8461 agcgatcgaa actgtccaga gcatcggcac cctccttgaa atgacgatga aggaaaaatg
     8521 agaacctttc gttgtcgaaa gggtctggta taccttatgt cgaaggaaag gaaacaaaaa
     8581 aactttcctg tactattcca aaaaggaaag aaagttatta tacagtaaaa aaagaaatta
     8641 ttcttcatgc tagagatcag tacacgttcg agcccaatat caaacactag ttccaggcag
     8701 gcgcaacagg tgcggttatc actgaatttt cttccagcag tgacgattca agtgatattt
     8761 ttacaaatcc agctatttat cgttaacaaa aagattttta ggagaagctg atgaacaaaa
     8821 atgatcaaat tttgatcgtt tagttcgtcg gcttttttat ttagagaaac atcatttatc
     8881 agtgaaacat aacacgagaa caaaatgatt ttttcgttaa taacagaaac aagtatttgt
     8941 tgtttaaaat aatacacgtt attggcatat aggacgaaat cccagtatct tctataagta
     9001 agtcatttca tatcgatgaa aacacgttat ttatttttgt tttatctata aaatggctga
     9061 aaaatgattt ttttctaaat actatatata gtggcgtcta tcctatatac cactaaaaaa
     9121 ctacaaaatc tagtctttta ttttaaaaaa acacgtgtag aatgaagata gacttttgag
     9181 ttagggagtg tttttttatg aaacttactg ttttttgcgg ttcccgtttt ggtaataaag
     9241 aaagttacaa atttattgcg caaacgttag gaaaatacat ggcacaagaa gaaatcgaac
     9301 tggtttacgg tggttcatac tccgggatta tgggagtgat tagtaaaaca gtactggaaa
     9361 ataacggaaa agtcacaggt atctatccga atgggctttt tgaaactgaa ttgcctggaa
     9421 aagatgttac tacatttatt cctacagaaa cgatcgatga aagaaaggta ttattatttg
     9481 aaaagggaga tgctgtcctc gttttccctg gaggtctagg tacattggag gaattctcac
     9541 aacttctttc ttggatagca atcggtctga cgcccgacaa gccaatcgga atattgaata
     9601 ttggcggtta ttacagcggc ttgcaacaat tattagaaac ttttgcaaaa gaggatttta
     9661 tggataaaaa atggttggat caagtaatct tttccaacaa tccattggaa ctggtcaatg
     9721 aattacgagc agtatctatt aaaaatcagt tattattgaa ggaggcaaaa taatgagtca
     9781 aatatattta gcaggaccat ttttttcaga ggagcaaatc gatcgagtaa gtcgtatcga
     9841 aaaagcgttg gaggaaaaca aaacagtcac aagtttctac tctccacgtc atcaccaaga
     9901 aagcaattat gaactattca gcgctggatg ggcgcaggaa gtatacgaaa aagatatgga
     9961 agaactgaca aatgcagaat ttgttgttgc tatccttgac tttgagcatc aaacaatcga
    10021 tccaggaacg gcttacgaat taggtgtggc aactatgttg aaaaaaccga tgatcattgt
    10081 acaagaagag acggtaccta cgaacttgat gattacacaa agtctccata cgtatttaaa
    10141 atcagaccaa gctgtacgtg aatatgattt tgaaacattg cctgttgaaa cttacgtagg
    10201 cgagtactta tagaagagga agggttgtat gtcagtgatt ttgatttcag gaacgattgg
    10261 tgcaggtaag agcagtttga cggatatgct tgcaaaagag attgattcta aaccatttta
    10321 tgaaaatgtc gaagataacg aagtattgcc gctcttttat tcaaatcctg agcagtacgc
    10381 ttttttattg caaatcttct ttttgaacaa gcgattttta gcaatgaaaa atgctttagt
    10441 gaacgatgac aatgtgctag accgatccat ctatgaagat agcctgttgt tccatctaaa
    10501 cgctgattta ggtcgggtaa cggatatcga agtacagcag tacgatagct tattagatac
    10561 gatgctaaac gagttagatg atgttgctcc aaaaaaacgg cctgatttga tggtccatat
    10621 caaagtttct ttggacacga tgctagaacg tatcaaaaaa cgtgggcgtg actatgaaca
    10681 gctagaaagc gatgagacgt tatataccta ttatgaaacg ttgaacaccc gctataatca
    10741 atggtatgaa gattttgatg tatgtcctaa aatacaagtg gatggggata aattcgattt
    10801 tgtagaaaat ccagaagatg caaaaaaggt gatccgtcag atccaagaaa aattagctga
    10861 attggaagga aagcctgctg gaaaatattt tacggataac cagcatgcaa aaggtcaaga
    10921 attaggctaa aaataa
//
LOCUS       DAIGOK010000092        10918 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.092, whole genome shotgun sequence.
ACCESSION   DAIGOK010000092 DAIGOK010000000
VERSION     DAIGOK010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.092"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            774..1247
                     /locus_tag="NOA01_001988"
     CDS             774..1247
                     /locus_tag="NOA01_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HCI0633171.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1256..1483
                     /locus_tag="NOA01_001989"
     CDS             1256..1483
                     /locus_tag="NOA01_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCI0633172.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2119..2490
                     /locus_tag="NOA01_001990"
     CDS             2119..2490
                     /locus_tag="NOA01_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633173.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2746..2988)
                     /locus_tag="NOA01_001991"
     CDS             complement(2746..2988)
                     /locus_tag="NOA01_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCI0633174.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3020..3922)
                     /locus_tag="NOA01_001992"
     CDS             complement(3020..3922)
                     /locus_tag="NOA01_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCI0633175.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(3935..4123)
                     /locus_tag="NOA01_001993"
     CDS             complement(3935..4123)
                     /locus_tag="NOA01_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCI0633176.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4137..4700)
                     /gene="amaP"
                     /locus_tag="NOA01_001994"
     CDS             complement(4137..4700)
                     /gene="amaP"
                     /locus_tag="NOA01_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCI0633177.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4738..5631)
                     /locus_tag="NOA01_001995"
     CDS             complement(4738..5631)
                     /locus_tag="NOA01_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCI0633178.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5709..6632)
                     /locus_tag="NOA01_001996"
     CDS             complement(5709..6632)
                     /locus_tag="NOA01_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCI0633179.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6681..7031)
                     /locus_tag="NOA01_001997"
     CDS             complement(6681..7031)
                     /locus_tag="NOA01_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCI0633180.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7064..7966)
                     /locus_tag="NOA01_001998"
     CDS             complement(7064..7966)
                     /locus_tag="NOA01_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HCI0633181.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(7959..8816)
                     /locus_tag="NOA01_001999"
     CDS             complement(7959..8816)
                     /locus_tag="NOA01_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HCI0633182.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9150..9959
                     /locus_tag="NOA01_002000"
     CDS             9150..9959
                     /locus_tag="NOA01_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCI0633183.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            9999..10496
                     /locus_tag="NOA01_002001"
     CDS             9999..10496
                     /locus_tag="NOA01_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCI0633184.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
BASE COUNT         3186 a         2054 c         1845 g         3833 t
ORIGIN      
        1 gctatttgat tgtatcaaat ctaggaaata tggtatttgg tatcagtaaa gaattcagtt
       61 ggactactac attatgggaa tctctattct ttttcatctt cgtatttttg ttacaaaatt
      121 atcgaaaaaa ataaacatgt gccatctatc tgttggcaca tgttttagga tttatggtac
      181 tttttattcg gcaatccatt ttatttttct acttcttctt aatacctcta acacttttga
      241 tttataaact aatccaatgc atctacttgc gcttattaag ctatttcagc tagatagtac
      301 caccccatgc gtcaatgctt gattttcctt ttgtataaca ttgtataagc aataaaggac
      361 aaataagtgg tgattacacc aaaccattga ataagaaaaa ttctgatacg taaataaaca
      421 tgaacttgaa aaaattaatg aatcggttag ctactacgta gttgtctttt ttctcataca
      481 gttctgttaa cttacacttc tcttcgctag ataataaaaa atcctccaac tgtattgtcc
      541 aatataatcc caggcatcta aactagcatt atagaagaga cacacataga atctaaaata
      601 actggtttta aatgtttctt acaatcaaaa aaagctgtta aatgcactcc acaccttcat
      661 actctatcat tttttataaa cgatatattt ttattgtttt tcgataaaaa atagttgttt
      721 tacaataatt attctgctat attttagcta tcaaagataa aagagggata aaaatgaaat
      781 ttaaaacatt gctattaaaa tttgtcgttt taggtataag tgccgtattt attttgttcg
      841 gcgtactttt ttatactcag atcgctacaa gcgaaaaagg attcaatttt gattggcaga
      901 ccacattatt tgtttctgtc atttactttg cccttctcct agggatcgtt attgcttatt
      961 tcttattgaa actattgaaa aaaatcgaag ataaccaagc tttttccact caaagcttaa
     1021 acatactaaa aaaaatcgaa cgaaccattt tgtttattgg tttcgccttt tttggcttac
     1081 ttcctaaatt tatgaaagta gcggatttag acgatgctcc aggtttgatg ttggtaggaa
     1141 tcgctgtcgt attcattcct tttgcagtat acacattaac aagcgtgctg gaaaaactgc
     1201 ttgaacatgc gattttactt aagacagatc atgatttgac agtgtgaggt gcgacatggc
     1261 aattatcgtt aatttagatg tagtattagc tcaaaaaaaa atgagtgtga ctgaattgag
     1321 tgagaaagtg ggcattacga tggcaaacat ctctattcta aaaaatggaa aagcaaaagc
     1381 catccgtttt tccactctcg aaaaaatctg ccaagttttg gagtgtcaac caggtgattt
     1441 gttgacctat aaaaatgact cgaatactat accagaagaa taaaataaag tagaacaaac
     1501 agtaaataaa aaataatctg aatgatagat gtgtgagact tttagaagtt atttacgtct
     1561 attattcaga ttttttattt cgattgaata ccgttttatc tataaataag atatgtactg
     1621 actcatacaa agggggcctt ttcttctcca tgtactatca cttttcctga tacaactggt
     1681 ttcctgttac gttgtagttg gatctgcttt tagtataata ctttattcat tccaccagac
     1741 tgatagcagt ttgagcagtc taaataatca tgtagtatta caggccattc tgtttcatac
     1801 cttgaagtgc ttaaaacttc agctaatggc ccattgtgtt aactcttact ccattctttt
     1861 ttagtaagct cccatatata ctgtaaagcc ttctatatat tatgtcatgt tgctttttaa
     1921 catttactat gtagttttga taaaatgatt tgaataacag tgatggacaa ttctttttta
     1981 tttttaacat tattttgata ataatgatta tttatttaaa aatacattag ataacatttg
     2041 ttacgacacg aaaaagcttc acaaacaaat atcgttattt taaaatgagg gcgaactcac
     2101 acaattagga ggaatagtat gccatttatt gtttactttt ttctttcatt actcgtgatc
     2161 tatattccat taccaacatt tttcctgctc gtcaatcgtc tagcagagca acatgatacg
     2221 attgctgctc ttacgaaact ttgtttactt ctatttgttt gtctagatta ctttacgagc
     2281 tattattggc taaaacagac aagagtaaaa aagagatatt atttcttcct tgtctttttc
     2341 aatctctttg agtttctgat ccattgcttt ttacttcatc agtatcatac gatccatttg
     2401 tggatcatct gggttttcaa agtggtcgta ttattttgta tgttcacctt tcctatctca
     2461 agaaaattta gggaggcatt gttttattaa agaagcttct ctgtatcaat aaagacacat
     2521 ttaaggaaaa acttttaaaa atctaattgg gaatttgccc gcataaataa gaggctgtga
     2581 caaaagtctt gtcacagcct cttattccgt ataaacagtg ttcaaaagct tgcatcttcg
     2641 ataatatgcc caaccaaatg aaaaatatga gaagctattt ttgtttggct gttcttatta
     2701 cttgatgaag aacagctttt tcacgacctc tttttaaaag tctttttaca gttttcgagc
     2761 aaagaatgat acgattagta ctaaaatgat ggcaccaatc aatgatggca cgattgccat
     2821 tcctgcaata cttggtcccc aagaacctaa taatgcttga ccaagtgctg atccaactaa
     2881 acctgctacg acatttgcaa taatccccat cgaagaacct ttattcgtga ttgctcctgc
     2941 aattgctccg ataattcctc cgacgataag tgaccaaata aatcccatgt ttcgttcctc
     3001 ctaatgtttt acttccttat tactgaaccc gtggttctgt attttctttt actttttgtg
     3061 cgccgcttcc agctgctgat tttactttat ctgtttgatt cgaagcaaat tcaccggttt
     3121 ttttcgctgc gtcagttact ttgtcttgca ccgtttcttg atctttttca aattcttctt
     3181 ttgttttgat gtccactacg ttagcgttga cttcgatcac ttctaaatgt gtcatatttt
     3241 ttacttcttt gccgattact tcttgcattt cttcaaagat tttttcgata tctttgccat
     3301 attccacaat gacatccata tcaactgcta cttgtttttt accgacttct gtttcgatcc
     3361 cagccgttac attatctgta ttcacaagtt ttcctgccac attagagaag aaaccaccgt
     3421 ttgcagataa taaaccgtct acttgttcaa ttgcgattcc gatgattttt tgcacaactt
     3481 tatcttcata attcagctca ccgcgcattg aatcttcctg ttcagaatca tgctttcctt
     3541 ggttatttgt atgatcagaa ccaagttttc cttgtccgtt tgtgtggtca gaactaagtt
     3601 tttcttgtgc tttcgcatgg tcagaaggat gtttttgctc ttccacaggt ttagaaggat
     3661 cgattgcatc atgttgttct ggaactttag attctggagt tcctggatga tgaggtgcat
     3721 gcttttctgc atcatgtttc ttctcttcta ctccttgttg aacgttaggg actactggat
     3781 ctggatgtgc agatactggt gctgttttgt tcttttcatc gttcacacca tttgattggt
     3841 ttggtgtcgt tgggtctgga tgtgcagaca ctggtttttg gttgttcatt ttattgttat
     3901 tatttttgtt attgttattc atggatgttt cctcctaaga tttaaaatag tgatctaaga
     3961 ttttcgtttg tttcagataa aaaccgatag cgacgccgat cgctgccatg atcaagatca
     4021 ataaggtctt gaaaaagccc acggtgatcc ataaaacggc taatatcaat ccaagtaccc
     4081 caaagatgat agggagcttg tatgtcgaaa atagttcttt catttttggt ccccccctat
     4141 tctactcgtt tgcggtcatt gcttcgttta gctggttctt caaagcctgt gtatttcacc
     4201 gatactttca ctggctcttt tgttcccaat atgtgttgta cttcattttc gatttctttc
     4261 atcaatgttc ctgttttacc aatgagtgct gaagttcgtt tcaactcccc ttttacattg
     4321 acggcgatct tgtttttcgt tgctttgaca tttacttttg gtgaatggat gaattcatct
     4381 tcatgcaagt gagaacgaac cattccttca attgcttttt tatccaacgt caactcccca
     4441 tcaccatgtt tcagtagaaa gtttccgatt gtttttggat aaaacaggaa aacaagtaac
     4501 gtgataattg tcaaaatagc aaatgcaacg gcaagccaaa atacatattg ttgcacgtat
     4561 gaattagtga tggaaaagaa tcgaaacttc tctagctgaa aaggcaatgg ataggttgca
     4621 cttaatagta tcgcaaacaa aagcacagat agcaaaagta atgttccgat cactccgatc
     4681 gtttttacac ctttattcat tttcgcacct cctcataggg gtgaaacgat caacttctta
     4741 taagttgatc gcttctccac cagttactcc gtaaatttgg gctgtaacat aacttgcttt
     4801 attcgatgct aagaaaacat aaactggtgc taattctcct ggctgacctg cacgttctag
     4861 taaactttgc tgaccaaatt cagggatcga atcagaaggt tgtccttcgt ctagttgtaa
     4921 tggtgtccag attggaccag gtgctacacc atttacacgg attcctttag gtgcaagctg
     4981 ttttgctaaa ccgatcgtaa agttagcgat tgaagctttt gttgctgcgt aatctaataa
     5041 atgttcgctt ggattgaatg cttgaacaga agttgttgtt acgattgctc ctcctgctgg
     5101 gatatgcgga acagcttctt tcactaaagc aaacatagcg atgatgttta tcataaatgt
     5161 gtctttgact tgctcaagtg gtaactcagc aatagactga caagaaattt gctgtcctgc
     5221 gtttagtacc atcgtatcta gcccgccaag ttcactcacg gctttatcta tgatttcttt
     5281 aggtgcttgt tcgtcacgaa gatcaaaagg tagtaagaca atcttacgtc ctgcatcttt
     5341 cacgtatttt gctacttcct ctgcatcttt ttcttctcct gggaagaatt gaagagcgat
     5401 atctgcccct tctctagcaa atgcaatcgc tgctgcacga ccgatccctg aatctccacc
     5461 agtgattagt acacggcggt tttctagttg attattgcct tcataggatt cttctccaca
     5521 atcaggaaca ggaatcatat tctgctgtaa agctggtgta tcctgatctt gtttaggaaa
     5581 tttctccgta tggtacaagt ttcgcgggtc gattaatttt ggttctgtca ttgctgtatt
     5641 cctcctagtt tttaaattgt attgccaaaa caaatttctt tgtttgatac ctgttttaaa
     5701 tagattcttt aatttgtata atctttccgg tataaatggt aacctaaaac tactgctgct
     5761 atcaaagagc caaagactac gagtataaag cctagagcag aacttttacc cggtaatcct
     5821 cctgtattca ttccccaaat ccctgcgaac aacgccggaa cggaaatgat taatgcagct
     5881 gaatctagat atttcatgat atgatttaga ttattgtcca tcatcccgtt gaatagatca
     5941 ccaatactct ctaataaatc tcgatagatc tcgatcattt tttctgtttg tcgctgtctc
     6001 aaatggacat catagagtag ctgttcatcg tctaaacgtt tgatgaaagt ttcagaattc
     6061 cataattgat ccattgtttc ccgttgatct cttaaggtat gatcgatata tacgatgtct
     6121 ctttcaagat ctgcctgttt atacagttct tcattttccg ttgttttacg agctgcttta
     6181 tccaaagcgt caattcgctt tttcatctct cttagttctt tcacgtaatg ggtgtaaata
     6241 tctaataatg tatacgtgat gatcttttca aacgagttta aatgatttcc atgctttgtc
     6301 agcagccgat ccacaaattc ggtttttcta tcgatacaag taatcaagag attatcggaa
     6361 agtacaaaag atattggcgt taattttttt tcgatacgat tatctgactg atctagatta
     6421 atcactacca aagaaaatgg atgctctagt ttcgtctcat aaaggcgctc catccgagaa
     6481 acttcttccg gatattcggt tcctacgaaa atatcatcag gaagctcgaa ttcttcgatt
     6541 gcttgctcaa tctcttcggg ctttgcacta tcaagtacga gccaattata ttttttttca
     6601 tctgtttcta ttacattctt ttcttcatcc acaaagtagt atctcaatgt atacctcctt
     6661 tttggacttt ctcgaaacta ttagctgaag aagcagttct ttaatttgat attataatcg
     6721 agtactgcga tttcattttc taatgaaaag ctgtcaagct gtttgttgaa gtccatgttc
     6781 aatcgtttac cgaaataatt ttgctctaat ttagatgtat agatgtccaa taatggatgc
     6841 tcgattttag tcgcatattc tccaaattcc gaaacaaaag gaatgatttg aaaaaagctg
     6901 ccttttgcaa aagcaccttt gtcccttaga aatggatcgg aatcattgat aagcgcgagt
     6961 gcgatttttg tattttcccc aaccttttca attaacgtct ctactgcatt tttagacatt
     7021 gtaatttcca ttcctactcc ccctcttcag ttgttgtctt ctattattta acaactacat
     7081 ctgtttttgg ttgaactttt gcatattttg gatatttagg gaatttagct acacaacgga
     7141 aagccgtcat ccctgcatat ttttcgtaat atttcttcgt tggcccttca aaatccttgc
     7201 tccacggttt cacatgtcct gaaaaatgga taatgtaagg attgttgcga gtttcttcgt
     7261 attcacgttc acctgctgct gttggatgtt tttttgcttt tgccatgata tagccttgtg
     7321 cattccaacg cggatgaagt ggtaaccaac ggtcatgtaa gatagcattt aatgcatctt
     7381 ggtcatggaa tcgtaatttt tctggattat gttcgatgaa atctaatact ttttgcgtaa
     7441 tattttcatc taaccatttt ttcacattga tcagcatcaa acctgagttg aaataacgca
     7501 ttgatttagc tgaaattttc attttttcca gtcgttgatg gaacccagcg tcttcaacag
     7561 ctgctacgat tgaatcaccg aagtcaagac gccataattt agagatatct tgtaaagcga
     7621 tcatgtcgca atccatatat aagatacgtt caacttctgt gccgcggaac aattccggaa
     7681 tcgcaatacg ataataagct gtttctggaa tacgatcgct gattaccaca tttgtaaaga
     7741 attctttatt gattttcaag aattccacat ctgagttcat tcgtgcgttt ttcactgaga
     7801 agcgtaaaag ctgtttactt tcaaaatcga tatcatcgtc aatcacatag aaccgaacaa
     7861 atttagacgg gttgcaatta tctaatactg agacaaataa tgcagctaaa tgtggtacga
     7921 attttgtgtt acaacttgcg actactgcta tttctttttt attcattgat ctcgcctact
     7981 ttcgttagag tactgtgtgc taattttttt aagtataaat tggtatacgg atgatctttc
     8041 aacgtattcc aaggtttatc atgtcctgta aagtgaacga tcgaaggttt ttcatttcct
     8101 tctttatact gacgttcata tttttcattt ggtgctggat gacgttcaaa aatcagcgaa
     8161 gtctgcatgt tccattttgg atgaagttgt tcccaatctt cataaagtac agcattcaaa
     8221 gcatcttggt cgtgatagat gattcgatcg ccattttcac tgaggtaatg gatagttttt
     8281 tctgtaattt cttttttatt ccattgatca atgtcaatca ccatcactcc tgaattgaag
     8341 tacaagtcat cggaatcgat tcctaatctt tccaaagctt tcacttggcc tggatcgata
     8401 atagcgccga ttgtttttcc atttaacggc tcatcatata gttttacgat atcgtctagt
     8461 actaacacat ctgaatctaa gtaaaggact ttcttataat cctctttagc taataagttt
     8521 ggtaaagaaa ttcttaagta agcggtagtt gtgatatggt cactgactag gaagtcttcg
     8581 tagatatctt tttccactgt caggaattgg atacttgcat tacttgaata tttatttacc
     8641 gtttcttcca atccttcttt actatattct gatagaccat cgtcaatcac ataaaatttt
     8701 atgcgtcttg ttttattgca attttctaat gcagttgcga tcatcacgct caagtaagga
     8761 gcataatttt cgtctgaagc agttaccaca ggaactacgc cgtatctctt ctccatcaaa
     8821 atcactcctt tttctaatct ttttctaacc tgttctacat ctagaatatt cctcatttgg
     8881 ttttaaagca aaatatatgc tttgttttca ttgaatattt taaaattttt ttatcacaaa
     8941 acgcatcggt aagcgctaac tattcgtttt tcatggcttt ttattcaaag gtattgacca
     9001 aaaactacta tatatagcca cctttttctt aagaatgttt tttgaaaaaa ttcataatga
     9061 caaagcaagt tagtttcatt tttttaaatt ctcatttaat cttaaggtaa caggaaaata
     9121 gatatgtaaa aagaaagtag gaagataaaa tgaaactagc agcgattgat ttagacggta
     9181 ccttgcttga tcgaaatgga aagataccag aagaaaatat ccaagcactt cggaaatttg
     9241 atgaaaatgg cggaatcgta accgtcgcta ccggaaggaa cagcatttct gctaaagata
     9301 ttttttccga attaggaatc ggtggctatt tgatttcttc caatggttca ctgattgctg
     9361 aaatgaagga tggaaaaatg gcgaaagttc tcagacgttc aaaaatagat agcgatacac
     9421 tgagaaaagc cttcttctat gctcaagaag cgaaaatatc catcattgct agccgagaaa
     9481 cccaagatga tcagctgaca ttcgatgaat caaatctagt caaagatgac ccttattatg
     9541 aacatttcaa tttacagaac cattcttttg atcaaatttt aaatatgttg gaagatcctt
     9601 ctcttagcta tcttaaatta gcattaacag ataagagtga agaaaaacta cagaagctac
     9661 aaagagatct gcaagcagat ggtatccaat cagtattctc tgatccgcat ttcttagaaa
     9721 tcacgcctac aggtatcact aaagctcatt cacttgtttt tctggcggat taccttggaa
     9781 tcgaatcgag tgacatcatg gcttttggcg atcaagaaaa cgacattgct atgttgaatt
     9841 acagcgggtt gagtattgcg atggggaatg cacagcgaca agtaaaagaa attgcaaacc
     9901 aagtaacact gacgaatgaa gaagctggtg ttgcttatat tttaaaaaac ttctattaaa
     9961 aagaataaaa tgacgatatg aatgtaggag gaaagaaaat gttagttatt attgatatgc
    10021 aaaatcatat tcttgatcca aacagcgaat tttatattcc agagtcagat caattggcag
    10081 aacgtattgc tcaaagatta gctaaagcac gccaaaataa tgaatatatt ctcttcacac
    10141 gggatatacc agttgaactg aaggacaaag aagaagaacg agaagatttg caattgatcc
    10201 ctcttctttc tccacaagaa aatgaacgag tgattaaaaa gtattacttt acgattccac
    10261 ctgaaacttt agctgaaatc caggaaactt tatttgaaag caaagaagaa caaaaagaga
    10321 tcgaagtagt cgggattgaa acaaacttgt gtgtactctc taatctgata ggcctgcaga
    10381 gtgcttttcc tgaagcagac ttttttgttg atccaatgtt agtctccagt agaaaacacg
    10441 gagaatccgc tttagagctg ttgaaagatt tcaacgtctc tatcgtcgaa tcataaaaat
    10501 atctatcttc atttacgata tttaacacac aataaaaaaa gcacgcagaa ctgttttagt
    10561 cctacgtgct ttttctatca aagtagattg gcttttttat gacacagagt gaaacggcag
    10621 gctcacgacc tctttcttaa atggtgttca atcagctgac cctcggtatt agcttaacaa
    10681 acggcaaaat atgaggaact atttttccgt ttgttaagct aatactcagg tcaaaacgct
    10741 gatttcacaa cctcttcctt aaacggtatt caaaagcttg catctgcagt aataagaaca
    10801 gccaagtaaa aacataagaa gctgttttta cttggctgtt cttattactt gatgcagggc
    10861 agctttttca caacctctta tgtaccttgt aggactcgaa cctacgaccg gacggtta
//
LOCUS       DAIGOK010000093        10639 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.093, whole genome shotgun sequence.
ACCESSION   DAIGOK010000093 DAIGOK010000000
VERSION     DAIGOK010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.093"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            23..646
                     /locus_tag="NOA01_002002"
     CDS             23..646
                     /locus_tag="NOA01_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633185.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1095..2144
                     /locus_tag="NOA01_002003"
     CDS             1095..2144
                     /locus_tag="NOA01_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCI0633186.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2141..3022
                     /locus_tag="NOA01_002004"
     CDS             2141..3022
                     /locus_tag="NOA01_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI0633187.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3025..3795
                     /locus_tag="NOA01_002005"
     CDS             3025..3795
                     /locus_tag="NOA01_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCI0633188.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4074..4928
                     /gene="aroE"
                     /locus_tag="NOA01_002006"
     CDS             4074..4928
                     /gene="aroE"
                     /locus_tag="NOA01_002006"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCI0633189.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNGTDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5106..6269
                     /locus_tag="NOA01_002007"
     CDS             5106..6269
                     /locus_tag="NOA01_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCI0633190.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6386..6796
                     /locus_tag="NOA01_002008"
     CDS             6386..6796
                     /locus_tag="NOA01_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCI0633191.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6970..9198
                     /gene="topB"
                     /locus_tag="NOA01_002009"
     CDS             6970..9198
                     /gene="topB"
                     /locus_tag="NOA01_002009"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCI0633192.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9369..9653
                     /locus_tag="NOA01_002010"
     CDS             9369..9653
                     /locus_tag="NOA01_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633193.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9813..10049
                     /locus_tag="NOA01_002011"
     CDS             9813..10049
                     /locus_tag="NOA01_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCI0633194.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10046..10444
                     /locus_tag="NOA01_002012"
     CDS             10046..10444
                     /locus_tag="NOA01_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HCI0633195.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3714 a         1772 c         2253 g         2900 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaatgaaa aacaaacgat taataacagt tgtagtaatt attttagttt
     1141 atttagcagg tgcatttatt tatggaaaag cctcaggcga aaaagaaagt acttcgacga
     1201 accagacaaa aaaaacaaca gttgttgggg tattgcaata cgtgagccat ccggcattag
     1261 atgagattta tcgaggaatc aaagatggtc tgaaagaaga aggattaaaa gaaggagaaa
     1321 atcttgagat caaatttcaa aatggacaag ccgatcagag caagctagcc acaatgagtc
     1381 aacagctagt acaatctgat ccagatgcct tgattggaat cgcaacacct gccgctcaat
     1441 cattagcaaa cacgacttca tcgatcccag ttgtgttagg tgctgtaacg gatcctgtgg
     1501 gggctggatt agtcgcttct ttagagaaac ctggtggtaa tgtaacaggt gtttctgatc
     1561 agccgcctgt tacttcacaa atcaagcttg cttctgattt gttgccaaat gcaaaaaaag
     1621 tcggaatctt gtatgcttct tcagaggata attcaaaata ccaagtagca caagcagaag
     1681 aagctgcaaa aaaagaaggg ttagaatcag taaaatatgc ggtgccttcc actaatgaga
     1741 ttgcacaaac ggttcaagtg atgagccgac aagtcgattt tatctatgtt ccattagata
     1801 ataccattgc caatgcgatg caaacagtgg taaaagaagc aaacaaagca aatatccccg
     1861 tcattccatc tgtggataca atggtggaac aaggtggact agcaacaatc gggatcaatc
     1921 aatatcaatt agggcttcaa agcggtaaga tggcggcaga acttgcgagt ggaaaagaaa
     1981 aaccggaaac gacgcctgtt tatacatttg atcaagggga tacagtgatc aatcaatctc
     2041 aagctgatcg cttaggaatc attgttcccc aatcgatgaa ggaaaaagcc aaaatcatta
     2101 cagatgactc gcagcaagaa acatctaagg aggatgacaa atgatcgtat cagctatttc
     2161 acaaggcatg ctttgggcaa tattaggatt agggattttc atgacttatc ggatcttaga
     2221 ctttcctgat atgacgacag aagggtcctt cccattaggt gctgctgtct gtgtgacagc
     2281 tattactcat ggggtagcgc cgattgtagc tacattatta ggcgtaggtg ctggcatgat
     2341 agcaggactc gttacggggt tactttatac aaaaggaaag ataccggtta ttttagcagg
     2401 gattttagtg atgtcaggat tgaactcagt tattttgttt gtgatgcgaa caccaaatct
     2461 ttctttattg aatcatccga ttctacaaga cagcttccat cgctttgggt tacctgatta
     2521 ctttgacaca gtccttcttg gcttcatctc tttgggcatc ttgattgctg tactgctatt
     2581 cttcttcaat acagatttgg gtcaaggcta tattgcgaca ggagacaacg aaacgatggc
     2641 tcggtcgtta gggataaaaa ccgatcgtat gaaaatttta ggtttaactt tgtcgaacgg
     2701 tgtcattgca ttatccggtg cgctgattgc ccagaacgac ggatatgcag atgtaaataa
     2761 agggattggc gtaatcgtca tcggtcttgc ttccatcatc atcggtgaag tgatcttcca
     2821 agaattgacg ctagcagaaa gattgattgc tattgttgtc ggcagtatca tttatcagct
     2881 attgatttta gtagtaatca aactagggtt tgacacgact tatctgaagt tgttctccgc
     2941 tatcatctta gctatttgtc tgatgatccc gcaattgaag aaagcactga acttaaaaac
     3001 gggttttgag aaggaggcat agtaatgact gatatattga caatcaaaaa tggcagaaaa
     3061 gtcgtaaaca atggaatgaa tgaagagaaa gtactattgg atgaaattaa ccttacaatc
     3121 agagaagggg acttcatcac tgtccttggc gggaatggtg ctggaaaaag cacactgttc
     3181 aatacgatcg ctggtacgct tcaactcagt caaggatcga tctcttttaa agatcagctg
     3241 atcacaaaag attcagaaga aaaaagagca cgattccttt ctcgagtttt ccaagacccc
     3301 aagatgggaa cggctcctcg gatgacagta gcagaaaatt tgctacttgc acaaaaaaga
     3361 ggtcaaaaac ggaaattccg tttacgtaac ttgaaaaatc aaaaagaaga ttttttcaga
     3421 ctatgtcaag aagtcggtaa tggcctagag caacacttgg atactccagc gggtgaatta
     3481 tctggtggtc aacgccaagc attaagcttg ctgatggcga cgatcacagc tcctgagtta
     3541 ctattattag atgaacatac tgcagcttta gatcctaaaa cagctaaagc attaatgcga
     3601 ttaacaaatc agcggatcaa tgaacaaaaa ttgacttgtc tcatgatcac gcatcgaatg
     3661 gaagacgcct tgaaatacgg aaatcgtctg atcgtactcc aaaaagggaa aatcgttcaa
     3721 gatatttctg aaaaagaaaa gcaacatttg acaatgggcg accttttgca gttctttgaa
     3781 gaagtagtgg attgatttcg acagaaaaat tcctttcagt agaaggactt gaaaactcat
     3841 tgacatcttc ctttgattca tcctatactt aaaacaataa acaattaaac atatttgggt
     3901 gttttgtagg atcacgtagt caatgatttt aacaattaaa tagaatattc tttgctctga
     3961 caagtaaacg atgagaaaag aatttgctac tttgccacct agacgtttcg tactttttcg
     4021 tatcaaacgc taagtggctt tttttattga agtaagaaca aaggaggaat aagatgattt
     4081 ctggacaaac caaattagct gggctctttg ctgatccggc ttctcacagt ttatccccat
     4141 tgatgcacaa tactgctttt gaagcaaaca acatcgatgc ggtgtattta gctttccaag
     4201 taaatccaat gaatttgaag caagcagtcg aaagtattcg aacttttgat atgttaggtg
     4261 taaatctttc gatgccgaat aaaacagcgg ttattcctta tctggatgag gtaagccagg
     4321 aagcgcaatt gatcggagca gtgaatacga tcgtgcacga aaagggtcgt ttgattggct
     4381 ataataccga tggtgcgggc ttcatgcaat ccgtgcaaga agctgggatt tcgatcaaaa
     4441 agaaaaaaat gactctactg ggagctggag gtgcagcaaa ggcagtcact gttcaagctg
     4501 cgttagacgg cgctgaggaa atcgtagttt ataaacgaaa caaccaaaca ttcgctcaag
     4561 taaaagaaac attcgaacaa ctcagctatc gaacttcttg taagataaaa gttcgtgact
     4621 ttgccgatac gcatcaatta agaaaagact tgaaagaaag ctgtcttcta gtcaatggaa
     4681 cagatatcgg gatgggaaag aaaatcaatc agtcaccgct cttagatata agcatgatgc
     4741 atgagaatct agcggttttt gatttgattt atgcacctag agaaacacgt ttattaaaag
     4801 aagcaaaaaa agtaggagca aaaggataca atggacttgg catgttgatc taccaaggag
     4861 caatcgcgtt tgaattatgg acaaaggaaa agatgccgat ccatgtgatt gaaaagctgt
     4921 ttgattaaaa aaggataata gataaagatc ttttattctc gcataaacgg tctcaagaaa
     4981 agtaatcctt tatgtatcga agcatttatt tcgttatttt ttattaaatt atcaaaaaat
     5041 gataggcttt tgtcgattgg ttatcgtatc ctttatttat cagtaagaaa cgaggaggaa
     5101 ccacaatgag tcggatggat cgctataagg acatccataa gaaggcgaaa cctctgaagc
     5161 gggatacggg gagtattttt gctagaagag aacgaaaacg aacgcgtatg gaaaatgagg
     5221 aagatgctac ccgtatttat gaaagagagc agcattcatt tgaacatttg aaccaatacg
     5281 agcaacagat atacagagat gcaccaagag cagaagaaca acaaaaaaat aaaaaatttt
     5341 tcaatcggca aaagaagaac aaaccgttaa aacctcgaag aaagcgatcg tggaaaaaaa
     5401 tcattctatc gattttgctt gttttgatcc tttattcagg tatctcattt tttattggta
     5461 aatatgtagc tgaacatgac acaagcttag aggcagcgac ttcagaaacc tttcatgggc
     5521 aaaaagcgac gaatggtgcg cataatattt tgatccttgg aagtgataca cgcggagaag
     5581 atgccgggcg agccgatacg atcatggtct tacagttgga tggtccttcc cataagccga
     5641 aactgatttc ttttatgcgt gacagcttcg ttacgatccc aggggtagga caaaataaga
     5701 tcaattcagc ttatgcatac ggaggagccg atctagtgcg tcaaacttta gtagaaaatt
     5761 ttggaatcga ttgtcaatac tatgcgaaag tcgatttcaa atcgtttgaa aaagtgatcg
     5821 atgccttatt catgaacgga gtgaagatcg atgcagaaaa agatctgaat ctggatggtg
     5881 tagatattaa aaaaggggtc cagaagatgg atggacatgt tcttttgcag tacgcacgtt
     5941 tccgaatgga tgaacaagga gactttggcc gtgtcagacg ccaacagcaa gtaatgaacg
     6001 ctattttcag ccaattgaag aatccattga atctgatccg tgcaccttat gcagctggta
     6061 aagctttagg atatacatcc acagatgtca ctgcttggtt cttgacgaaa aacctctttt
     6121 cgattgcacg aggagctggt ggcattgatc gaataagtgt gccagtagac ggttcgtgga
     6181 attatgggca aagtgattat gctggcagta ttcttgaaat tgataatcaa atgaatcaat
     6241 ctgctattac agattttttg aataaatgat cataaaaaag atcaagaaga agctgcgcaa
     6301 ggcttttgtc atgaatcaat caaacgattc actcgtaaaa aacgctttct tatggtagat
     6361 ttaaccaaga gaggagctgt ttacattgga tatctatctg aacttcaaaa atgagtcgcg
     6421 tgaagccatc gctttttatg aaaaagtttt tcagactgaa gcatcaggta tcaccacata
     6481 cggtgaaatg cctggaatag aacaaatgaa cttgccagaa gaagcaaaaa atctaatcac
     6541 gaatgcacgg ctgaacattt atgatacatt agttatgttt gcagatacgc cagaaggaat
     6601 tggtcctgaa ttagttgtgg gaaataatgt ttctttagtc atccagacaa gaaatgaaga
     6661 agaaacgaag cgcctatttg aaagcttagc tgagggtggt aagattgaga tgccactgga
     6721 tgctgttccg tgggctcagt tgtatgggat ggtaactgat cgatttggca tcaactggca
     6781 gattaaccaa gagtgacagc taaagatgaa tattttctgg aaacatcaag cagcaaacgg
     6841 atcatcgttt gtttgcttga tgtttttgtg tctatttcga ctatacggta tctttttttt
     6901 attgccagat gcgaaaatat gttccataat agagaagtcc gcaaaaaatt aagtaacgga
     6961 ggtcagaaga tgaagacggt gatactagct gaaaaacctt cacaagcaaa agcctatgcc
     7021 gaaaccttcc aacagcaaaa gcggcatgaa gggtattatg aaatcagcga tccattgttt
     7081 gctggtgaag taaccatcac ttatggtttt ggacatttgg tagatatggt tgctccagga
     7141 gcttatgaag agcgttgggc aaaatggtct ttagaaaatt tgcctatttt cccagaaacc
     7201 ttccgttacg aagttccaat ggacaaacgt gcacaatttt cgatcgtaaa acgagaattg
     7261 cagtcagccg ataccatcat tattgcaaca gatggtgatc gagaaggtga agccatcgcg
     7321 tggtcgatta ttcgacaagc aaacgcattt tctaaggaaa agaactattt acgtttatgg
     7381 atcaattctc ttgaaaaaga agcaatctat gaaggtttta agaatctcca acctggaaag
     7441 aactattttc caaaatataa agaagcgcaa gcaaggcaga acgctgattg gctgattgga
     7501 atgaatggaa gtccattata tagcttgcta cttcaacgaa aagggattga tggcagtttt
     7561 tctcttggtc gtgtacaaac accgacactc tatatgattt atcagctgca agaagaaatc
     7621 aaacacttca aaaaagaaac gtactttgaa ggagaaggtt taattacgac aaacaaaggg
     7681 cagttttctg gaaaaatcgt accaaaaaaa gcatttaaaa cccaagaaga gttaacgaag
     7741 gaaatagaaa aacttggagc gcatttaggt aagcaaaacg gacggattct tgaagttacg
     7801 aaaaaggaaa agaggttgca tagtccacgt ttattttcgc tatctagttt gcagacaaag
     7861 atgaatcagc taatgaaagc aagtgccaaa gctacacttg atgctgctca aggattgtac
     7921 gaaagcaagt ttctaagcta tccaagaacg gattcctttt acattacaga aaatgagcat
     7981 cagtatctgg ttaaacatct gcaaaattat aaaaaatttt taggaataga agaagtacag
     8041 acaactgaga tcaaagcaaa gaaacgatat gtcgatgcga aaaaagttca agaacatcat
     8101 gcaattattc cgacaaagac gattccgact cctgcacgct ttgcgaagct ttccaaatta
     8161 cagcaagcaa tctatttgca agttttgcgc actactgtgg ctatgtttgc tgcagattat
     8221 atctacgaag agacgaccgt aatgacgggc gtacagcaat taaaactaaa aagcatcgga
     8281 aagatcccat tacagcaagg ttggcaggaa atcttacgca tcaagtcaaa aaagaaagaa
     8341 gctcagactc ttcctcctat cgtagaaaat gaagaagcag aagtagaact gaaaagtatg
     8401 gagaaagaaa cacaacctcc aaaaccctat accgaaggaa cgttgatcac agcaatgaaa
     8461 actgcgggaa aaacgctgga taatgatgaa gcgcaagaaa ttctcaaaga agtagaagga
     8521 atagggacag aagctacacg agccaatatc attgaaaatc ttaagcagaa gcattacatc
     8581 gaagtcaata aaaacgaaat aactgttacg ccaaaaggaa tcactctttg taaggcagta
     8641 gccaatgaac cattactgac gagcgcagaa atgactgcgc gctgggaagg gtacttgaga
     8701 aaaattggta aacaagaggg gacaccatct gtttttttag agaatatcaa aaaattcatc
     8761 ctacatttat tggcagaagt tcctaatcaa atcgacaagg tagacttttc agatgagatt
     8821 agcgggacac gtcagataaa agcgatggag aagaaaaacg atcaattagg aagatgtcca
     8881 aaatgtaaaa agggaatagt catgctttat cctaaaatcg ctacatgctt gaatccggaa
     8941 tgcgacttca aattgtggcc aacagtagct aaaaagaagc tgactaaaac gaatctcaga
     9001 gatttgcttt caaaaggaaa gacaagtaaa gttgtcaaag gttttactgg taaaaaaggg
     9061 aaattcgatg cagttttagt attaaaagaa gatatgacaa tcggtttttc ttttccatgg
     9121 atcgaaaaca gtggaacaaa agcagttgat ggaaacaaag aaacatcaag aaaaaataat
     9181 gaaaacgaag tagagtaaat gaaaacaatg aaatagcgga atatttattc cgaaaagtga
     9241 gaatataaat accaataaaa ctaaaaaata tcagtgaatc aagcaaaaac gttggggtag
     9301 gctgatttta tatagatagc aaggagtttt tttatttgaa aaaagtgcat tcatttttaa
     9361 attatcttat gggagcaatc acgattgtgg ctgtgtacca agcatttcta aagtatcctg
     9421 attacgggac gaagttgttg tcattgacgc acaatcaaac agcatattca ccaacgttga
     9481 ttttatcgac tttgactgca ggagctaaaa gcgctactta tttttttcat aaaacgacaa
     9541 gcatgctaaa gcgaaagaaa cagcgaaaac aattgttggt ggtggtagcg gctatgatca
     9601 ttatttattt tctaccagtt attacacaag tatttcatca atattttggt taagaatacg
     9661 agagtttgtg acaaaagtca tgtcacaaac ttttttcttt gtcatttttt cgtaagaaaa
     9721 agcaaaataa aagtatatac attgattaaa agtatatact tggatataat gtacttacta
     9781 tagaatgaga atggtataga gggtggcgaa atatgaaaaa aatgaaaaag tataaaatta
     9841 gaaaatctgg caatagcgat gtaacaacta tcccaccaga agtaaaagaa ttcatgggtg
     9901 tgcagacagg tgacgcaatc agttatgttt ttcaatctga tggttctgta cgaatgatca
     9961 aagcacaaga agaacctgat attgattcat tagttgattc gatcatgaat caatatgaag
    10021 acgcgttaaa agatttggtt gatttatgag gtatctttcg actaaagaaa ttatcaaaat
    10081 caacgcgaaa gttattttac gtcactcgcc aggcgaaatg atcggtgtaa aagatgcaaa
    10141 tgcattagat atggccgtca aacagccttc acaagcagta ttcaatcaag aactctatcc
    10201 tgaaatctat gaaaaagcgg cgattttagc aattaattta gctaaaaaac atccttttca
    10261 taatggcaat aaacgaactg ctcttgttgc catgttgttg tttcttcaat tgaataattg
    10321 tccagttgct ttttctcgtc aagaagctgt tgattttatc ataatgatta ccactagttc
    10381 attggatttc gattcactaa agtccaagat aaccagttat ttacgacaaa ccgcaataaa
    10441 atgaacgaat taaaaacttt atttctcaat tagatcagca tgtcattaag gaatatgctg
    10501 atttttttat gtttaatcat acaaaatagt tttttcataa aaatatggaa aatatggatt
    10561 aattttgaaa taattgattt aaaaggaata caaaagagtg aatagtagga aatagtggtt
    10621 tagaactagt gggctgatt
//
LOCUS       DAIGOK010000094        10593 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.094, whole genome shotgun sequence.
ACCESSION   DAIGOK010000094 DAIGOK010000000
VERSION     DAIGOK010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.094"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(217..702)
                     /locus_tag="NOA01_002013"
     CDS             complement(217..702)
                     /locus_tag="NOA01_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HCI0633196.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    792..1137
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1182..1823
                     /locus_tag="NOA01_002014"
     CDS             1182..1823
                     /locus_tag="NOA01_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCI0633197.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            1842..2477
                     /locus_tag="NOA01_002015"
     CDS             1842..2477
                     /locus_tag="NOA01_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCI0633198.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2495..3310
                     /locus_tag="NOA01_002016"
     CDS             2495..3310
                     /locus_tag="NOA01_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCI0633199.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3629..5065
                     /locus_tag="NOA01_002017"
     CDS             3629..5065
                     /locus_tag="NOA01_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCI0633200.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5254..7701
                     /locus_tag="NOA01_002018"
     CDS             5254..7701
                     /locus_tag="NOA01_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCI0633201.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7931..8998
                     /gene="rlmN"
                     /locus_tag="NOA01_002019"
     CDS             7931..8998
                     /gene="rlmN"
                     /locus_tag="NOA01_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCI0633202.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9191..10294)
                     /locus_tag="NOA01_002020"
     CDS             complement(9191..10294)
                     /locus_tag="NOA01_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCI0633203.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
BASE COUNT         3499 a         1807 c         2292 g         2995 t
ORIGIN      
        1 atttataatc gaaaaaagaa gcatttttat atatgcttct tttttcgtag aatcaataaa
       61 tatggatttg gctaactatg gaagaatact cgtattagtt ttcagcgttc atactagttg
      121 ttacattgtt tgattggcca aaagaatttc tgatatattt tgtgctaaat aaagaaaaag
      181 acgcttgaat tgggaaataa gaaaactact ctatcattac aaattttcat aaactggcag
      241 atgttctaag taaacaagta tcttttcaaa gtcggaaaaa tccataatca acgtagtggt
      301 attgatattt ggatgaaagc ctacggtatc ttgacgatca atagcagcat cgatcacgac
      361 ttggattttt ttctctttgt cgtgcatcaa tgctaatgga gtcactgtac ctgctggaac
      421 gcctaggagg tcaggaagct gttcttcaga aacaaaagat aagcgctttt cttttaattg
      481 ttcagctagt tgttttaaat cggcgtgctt ttcatcaggc aagatgacaa gatagatttg
      541 ttttcctttt cttgatttca ataatagatt ttttacttgc ggaccaggta gggaaaaagg
      601 gacatttttg actgacgtaa tcgcaggatg atcgactttt tgaaaagaga tacccagttt
      661 ttttagtagt tcataagctt cttgttcggt tgcacgactc atcgcgcatc accctccttt
      721 ttttattagt gtatcataaa aaagagttga caaatgaaaa ataatcatgt aaattacacg
      781 taaagttaaa acaaatgaca tcgaaaagaa gagtagccgt taggtgtttt tagagagtct
      841 ctggttggtg gaaagaggca gcagttaacc gtgaaacaca tcttggagtc gattttctga
      901 agcgtatagt aggaaaatcc ggggagcccg ttacagctgc ggtcgttgct agatgaccag
      961 ataaggctgg cttttccgtg aggagtcggt aaaaaaaggt ggaaccacgt gattaacgtc
     1021 ctttgatctt cggatcaaag gactttttgt tttcatctga acggcaacga ctgtgtgagg
     1081 ttttttctgc aaggaaaaaa taaacagagg tggaaccacg tataatacgt cctcttggca
     1141 tttgccaaga ggattttttt attttaaagg aggaaaagaa gatggagtat ttgattgaag
     1201 ttatgccgca attattagta ggagcactca caacattgaa attattttta tttaccttga
     1261 ttggctcatt accattaggg attgttttag cattaggatt gtctttgcat tttaagccgc
     1321 tacgctactt gcttcaaaca tatgtatggg ttatgcgggg aacaccgctg ttgttgcaac
     1381 tgatttttat tttttatggg ttgccgacga ttgggatcgt ttttgatcgt ttcgaagcag
     1441 cactcattgc ctttatctta aactatgcag cttattttgc agagatcttt cgtggaggga
     1501 tacaatcgat tcctcaaggt caatatgaag cagcgcaagt tttgcaatta agtccctttc
     1561 aaacggtgac gaaaatcgtc atcccacaag tgacaaagat tgttttacct tctgtaggga
     1621 atgaagttat caacttggtc aaagattctt ccttgatcta tatattaggt ttaggtgacg
     1681 taatgcgtgc tgggaaaatc gcgatggaac gcgacgcgac tttactgcct ttgctaggcg
     1741 tggcaatcat ctatcttaca ttgactggta ttctaacaat cgtaatgaaa caagttgaag
     1801 gaaaattaaa ttattaccgc taactgatag gaggaagaaa tatgattgaa ttgaagaaag
     1861 tgaacaaagc atttggtgaa aaaaaaataa tcaaagattt gaatctaatc attccagatg
     1921 ggaaaacatt agctatcgta ggtccttccg gtggcggtaa aaccacgctt ttacgtatct
     1981 tggctggctt agaaagttct gacagcggag aattttattt agacggtgta ccgttcaacc
     2041 cattcgagct taaaatgaaa gaacaagttg taggagtcgt attccaagac ttccagctat
     2101 ttccacatct gaatattttt gaaaacatta ccttagcacc aaaaatggta ttgaaacaga
     2161 caaaagaaga atacaccaaa aaagcagaag aattagcggc ttcattaggg attagtgatt
     2221 tattgaatat ctatccttat cagctttctg gcggacaaaa acagcgggta gcgattgcca
     2281 gagcattggc tatgaatcca cgggtattgg cttatgatga accaacaagt gctttagatc
     2341 ctgaattacg ccagcaagtc gagcgtttga tcctatcatt gaaagaagat ggggtaaccc
     2401 aaattattgt cacccatgat ttagtatttg cagaaaatgt cggtgatcaa ttactaaaag
     2461 tttccccaat tcattaagaa aggagaagat agagatgaaa aaaaccagtt ttctattcgc
     2521 tttgatcgca ggtcttcttc tttttgcagg ctgttcgaac aaaaaaactt cagcagataa
     2581 ttgggcgact atcaacgaag agaaaaaaat cgttataggt ttagacgata catttgttcc
     2641 tatgggattt agagataaag acgggaagct cacaggattt gatattgatt tagccaaagc
     2701 agtctttgaa caatatggga ttacggtaga ctttcagccc attgactggt caatgaagga
     2761 atttgaactg aacaatggaa cgatcgattt gatatggaac ggctattcca aaacgccggc
     2821 aagagaaaag aaagtgcaat ttacaaaacc ttatatggaa aacgatcaag tgctgatcac
     2881 acctaaagaa tcaggaatca caaactttgg acaaatgaat aataagagat taggtgcgca
     2941 aaatgggtca tctggttatg atgtatttac gaaacaacca gaagtattaa aagagatagt
     3001 caaaaaccaa gatgctgttt tgtatgatag ttttaatgaa gcattgatcg atctgaaaag
     3061 cagacgggtc gatggtctgc tgatggataa agtatacgca gactattatt tgaaacaaag
     3121 aaacgaacta gaagacttca acataacaaa aggttcttat gagagtgagg attttgcagt
     3181 tggtgcacgc aagtcagata gtacattagt gaaaaaaatc aatgaagccc tagatcaact
     3241 agagaaaaca ggtgaattcc aaaaaatatc cgataaatgg tttggcgaag atgttacgcc
     3301 gagaaattaa caaatagggg actgtgacaa aagacgtgtc acagtccctt tttccgaata
     3361 aacagtgttc tcctgccaac tccttgtgac tcggagctga atggcaggct cacaacctct
     3421 tggtaaacgg tgttcaaaag ccagtttttc ggtattaagc caaatttatg caaaaatgta
     3481 gaaagccatt ttcacaaaat ttttcttact actcgaaact aagcgacttt ttcacaacct
     3541 cctttacata attttatttt gttgtggaag gggtttcgtg ttattctaaa agaggaacga
     3601 ttgaaggtta taggaaagga agagatgaat ggaacacaaa gaacttacct catttccaaa
     3661 agattttctg tggggctctg cttctgcagc gtatcaagta gaaggagctt ggcaagaaga
     3721 tgggaaagga gaatcagtct gggatcgatt tgtacggata cctggaaaga catttaaagg
     3781 aacgaacggt gatttggcag tagaccacta tcatcgttat aaagaagata ttgctttaat
     3841 gaaagaacaa ggtctgaaag cttaccgctt ctctgtagct tggacacgga tttttcctaa
     3901 tggacgagga gaagtcaatc aagctgggct cgctttttat gagagattga tcgacgaatt
     3961 gattgaaaac gatattgaac ctatgttgac tttatatcat tgggatcttc cacaagcatt
     4021 gcaagatgaa tataatggtt gggaatcacg tcagatcatt gaagatttca ctcattatgc
     4081 agaaacatta tttgaggcat tccgcgggaa agtccattac tggattagtt tgaatgaaca
     4141 aaatatcttc acttcattag gttatttgtt agcagcacat ccgccaggtg tgacagaccc
     4201 gaaacgaatg tatgaagtta accatattgc taatttagca aatgcatctg tcatcaataa
     4261 atttcacgag ttagaaatgc ctggaaaaat cggaccaagc tttgcgtata cgccaaatta
     4321 tccaattgac agtaatcctg aaaatgttct ggctgctgaa aatgcagaag acctgatggc
     4381 caattattgg atggatgtct atatgtgggg aaaatatccg attgctgcca tgaggttctt
     4441 agaagaaaaa ggatgggcac caacgattga agctggcgat gcagaattgc tagaatctgc
     4501 taaaccagat tttcttggga tcaattacta tcaaacggcg acgaatgcat tcaatccatt
     4561 aaacggagtt ggtgctggaa aaatgaatac gacaggaaaa aaaggttcat cagaagaaac
     4621 tggagttcct ggaatgtata aaaaagttga gaatccattt gtcgaacgaa caaattggga
     4681 ctgggagatt gacccagaag gattaaggat cggactgcgt cgtatcacaa gccgttaccg
     4741 catgccggta ctgattacgg aaaatggctt aggagagtat gacaagttga cagaagataa
     4801 acaaatccat gacgactatc gaatcaatta cttacaaagc cacgtcaaag ccatcaaaga
     4861 agcaatttcg gatggagcag aagtcctagg ctattgcacc tggtcttata cagatttgct
     4921 tagctggctg aacggttatc aaaagcgata tggatttgta tatgttgatc aagacgaaac
     4981 acaagaagga tctttggaac gatataagaa agacagttat tactggtacc aaaaactgat
     5041 tgaagaaaat ggagagaacc tttagttcac atacttaaaa caagaaggta cggctggata
     5101 tggccgtgcc ttttcttgat aaaatcattt agaaattaag aaaaactgta aaataagctg
     5161 aaaaatgaaa gaaagcgtca gttctttcaa acaaaacttt agttccctta cttttttttg
     5221 tataatgaaa cttgatgaaa tgagggataa taaatggcac aaaagaaacg gcctactaaa
     5281 aaaaggaaaa agacgaaaaa aacgaaaaag caaaaacagc agcaggaaca aatgctgacg
     5341 attggtgttg ggtttgcctt gattttattt gctgtcttcg gctttttaaa attaggtttt
     5401 ttaggaatat tgattgcaaa cggctttcgg atcattgcag gaaatactta ccaaatcctc
     5461 tgtcttttat tagccgtatt aggattttgg attgtcatta agaatacaga gttttcaatt
     5521 ggtaaaaacc gacgctggtt tggcggcata ctcttttatt tcggtatctt attattgtta
     5581 catgcccatt tatttggcaa actccatact ggggaaccaa atatcatggg gacgacatgg
     5641 gatttgttag ccagtgatat aaaacaaagt caagtagaca ataacgttgg tggtgggatg
     5701 attggagcaa tcctttatca ttttacttac ttcttgattg cacagcctgg aagttattta
     5761 gcagcagtgc tattactggc aggaggagca ttcctgttca gtaatctgga aggctatcag
     5821 ctgttaaatg gtatccaatc aattggagaa cgagtacagg aattattgga aggtgatcct
     5881 gccaagcaag ctcgaaagca agcggcaaag gaagagagaa tgaaacagcg cgcagaagcg
     5941 aaagaagcaa ggcgcttagc tgcaaaagaa gctgcagaaa aagaagctgc agaatatgag
     6001 aagaaaaaag cggtgagcca gcaaagggaa aatactgctg atgaatggca ggaaccagaa
     6061 aatacagagc cggagcagct aagttttgtt ccaatcgaca gttttcaaga aaatatccat
     6121 cctgcgaatc tagagaaacc agtaccagat acaccaaaac agaccaatac agcagaaggt
     6181 ttttcagatg agttgccaga ggatgacggt acctcattag aatttgagat tgaagcagaa
     6241 caggaaaatc aagactatga attaccgaca gttgatttgc ttgattctat accaactgtg
     6301 gatcaaagtg atgagtataa aaaaattgaa aaaaatatcg gcgtattaga acaaactttc
     6361 caaagtttcg gtgtcgatgc caaggtcgtc aaagccagct taggaccatc cgtcacaaaa
     6421 ttcgaagtcc agccggctgt aggggtaaaa gtaagtaaaa ttgtgaactt aacagatgat
     6481 atcgcgcttg ctttagcggc gaaagatgtc cggatggaag caccgattcc aggtaaatca
     6541 ctgattggta ttgaagtacc aaatggaaag atcagtatgg tttcctttag agaaatcatc
     6601 gaagcacaac caaaccaccc agataaatta ctagaagtac ctttaggacg tgatgtttct
     6661 ggacgggtac aaacggctga tctttcgaag atgcctcact tactcgtggc tggttcgact
     6721 ggtagtggga agtcagtagc aattaatggg attatcacaa gtatattaat gcgtgctaag
     6781 ccccatgaag taaaactgat gatgatcgat ccgaaaatgg ttgaattgaa tatgtacaac
     6841 ggcatacctc atctgttgac gccggtcgtg acgaacccta gaaaagcagc gcaagcctta
     6901 caaaaagtag tacaagaaat ggaagaacga tatgaaaaat tcgcggcaac cggagtaaga
     6961 aatatttctg gttacaatga atttgttcaa cagaaaaact tggaaaacgg aacgaaacat
     7021 ccaaccttac cattcatcgt agtgatcgta gatgaattag ctgatttgat gatggtagcc
     7081 agcaatgaag ttgaagatgc gattattcgt ttagcgcaaa tggctcgggc agcggggatt
     7141 catatgatcc tagctactca acgtccaagt gtggatgtta tcacaggaat catcaaagca
     7201 aatgtacctt ccagaatggc atttgcggta tcaagcggta cagattctcg gacgatcatt
     7261 gattctaatg gggcagaaaa gctcttaggg cgaggagata tgctattctt gccaatgggt
     7321 gagaataaac cgatccgtgt gcaaggagca ttcatttctg accacgaagt agaacgggtc
     7381 gttcaatttg ttacagatca gcaagaagct cattatgaag agaaaatgat gccgacagat
     7441 gaagttgaaa cgacaggtgc tccagaacag ccacaagatg agctgtttga agaagcaaaa
     7501 gcacttgtcg ttgaaatgca aacggcaagt atttcccttc tccaaagaag atttcggatc
     7561 ggttacaacc gagcagcacg tttagtagat gaactggaag ctcatggagt tgttggcccg
     7621 tcagaaggaa gcaagcctcg gaaagtattc attgagcagg aagaaacatc agaaacgttg
     7681 ccggacgata atgacatgta aaacttgtgt gatgaagggt tgaaaaacga ataaccaata
     7741 aaaagacaag gcatgatgat accaaaagaa aaaatggttt ttttccatct tttctaatac
     7801 ggtatgatgt cttgtctttt tagtttttgt gagattaaag ttctattcga tttatttgtg
     7861 aagtattgca ttaaggaaat gaatcatgta tagtatgtag cgaaactttg agaaaaagag
     7921 ggaaaatact ttggaaaaac agtccattta tggcttgacg aatgaagaat taatcaattg
     7981 gtttatagaa aacggcgaga aaaaattccg agctgcccaa gtttgggaat ggttgtatca
     8041 aaaacgtgta agtaatttca cagaaatgac gaatctatcg aaacaattga tcgaaaaatt
     8101 atcggagcat tttatcatca atcctctaaa acagatggtc gtccaagaag cttcggatgg
     8161 tacagtaaaa tatttgtttg aactccctga taaaaacatg attgagactg tactgatgag
     8221 acaagaatat ggcttgtcag tttgtgtgac gacacaagta ggatgtaata tcggctgtac
     8281 gttttgtgca agcgggctgc tcaaaaagaa ccgtgatctg actgctggag aaattgttgc
     8341 acagatcatg atggtccaac attattttga tgagcgcaat cttggcgaac gcgtttcaca
     8401 tgtcgttgta atggggatcg gtgagccatt tgataattac gacaatgtaa tggacttttt
     8461 gcatatcatc aatgatgcca aaggattggc aattggtgct cggcacatta cagtttccac
     8521 tagtggatta gcacacaaaa tcaaggaatt cgctaataac ggcttgcaag tcaatctggc
     8581 tatttccttg cacgcgccga ataatgaggt gcgaacatcg atcatgcgga tcaaccgaag
     8641 tttcccaatt gagaaattga tggaagctgt ggatgagtat ttagagaaaa caaaccgtcg
     8701 aatcacgttt gaatatatca tgttgaatca agtaaatgat agaccagagc atgcgcaaca
     8761 attagcagat ctattaaaag ataagaaaaa attagcatat gtcaacttga ttccatataa
     8821 tccagtcagt gaacatgatc aatattctcg cagtccaaaa gctgatgtat tgagatttta
     8881 tgatgtattg aagaaaaacg gcgtaaactg tgtgatacgt aaagaacatg gtacagatat
     8941 tgatgcagct tgcggacaat tgcgaagcaa gcaaatgaaa aaaacagttg ccaaataatc
     9001 gtatcctact cgttttttca attaccataa aaggagcagt tttcttaatt aaggaactgt
     9061 tcaacttaaa taagagaaaa gattctatag cgaaagctat aaagcttttt ttgacaagaa
     9121 aaaccctcat caagcaaaga aatgtcctca atttgatgac agcctgcttg atgggggttt
     9181 tagcaattat ttaactgatt ttcttatatc cttgctggct ttcttgtgta gcatgataag
     9241 tttctacgtc aagaatggcc tgtttaaatt tttctcgggt gatttcataa ggcacatgtt
     9301 taatatcatt tgttgccatg gctttatcta agattgcttc taaatcggtc gtgacattga
     9361 gattcatgtc ggttaacgat actggaagtt ggttcttttt cataaacaca tagattttat
     9421 caaacaaatc gaactgctga tccatcatca ataaggtcag tacaccataa ctgacagatg
     9481 ctccgtgaag gtatccctcc attttttcta gaacagtcgt accgtaatag aatgagtgtg
     9541 ccatagcaga attatagtta ttgttaacta aaacagaaac caatccggta gtaacaatga
     9601 tatccgaaac gacttgttca attgcatatg agggacgatc gtcggcacta tctttcaatg
     9661 cttgcgtacc ataagtcaag agacgatcaa tgcacatttt tccgatttgg attcctaatc
     9721 tatccgtgta gcctagatca tcgccacgag cagaaaaagt agattcgact tctttggata
     9781 aagcatcgcc aatacctgcc cataagtatt tatcaggtgc ttcggcaata attgctgtat
     9841 caatgaaggt gtgtacaggt ggttttgaag gaaagaagag tccgtccatt acgccttctt
     9901 cgttatagat gacacatacc gcagtgacgg gcgaacagtt cgaaccaata gtgggaaagg
     9961 tgaagactgg tttagctagt ttttcaccaa tgacttttac agtatcgcaa gcacggccac
    10021 caccgactgc aaacaggacg tctgcttttt ggacactttc atgctggatc aactgttcaa
    10081 tatttccata agttgaatta cccccgtacc agagactggc gacaacctct atttcctgtg
    10141 tcaaagctac ttttattttg ttttctgcct ttgaaagtgc agtcttccct ccaatcagca
    10201 ctacttttgt cccatattct ttgacgattc catttgcttt ttcatacgca tctgttccaa
    10261 tggtataatt tggtaagaaa acactttgac tcatattcat ttccttcttt cttcgttgaa
    10321 acgggttaaa attttaaaga gtgatcgatc tgactgcggc tgtagatgga gtagaggtga
    10381 acctaagaca ggataagtga tgaataaggt ggcatgaaca ccttgaaatc atcaataagt
    10441 atcctaaaaa cgaacaagta caaaaacatt gaaaacgaca ataatgaatt tttgttcctc
    10501 ttagtatttc taccgattcc ctttgctttt ttgtaattat ctaaaaattc taaaaattaa
    10561 gattattata tgaaaacagg ttttatttgt caa
//
LOCUS       DAIGOK010000095        10590 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.095, whole genome shotgun sequence.
ACCESSION   DAIGOK010000095 DAIGOK010000000
VERSION     DAIGOK010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10590
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.095"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            108..311
                     /locus_tag="NOA01_002021"
     CDS             108..311
                     /locus_tag="NOA01_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCI0633204.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(412..1074)
                     /locus_tag="NOA01_002022"
     CDS             complement(412..1074)
                     /locus_tag="NOA01_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0633205.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(1087..2589)
                     /locus_tag="NOA01_002023"
     CDS             complement(1087..2589)
                     /locus_tag="NOA01_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI0633206.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            3137..4816
                     /locus_tag="NOA01_002024"
     CDS             3137..4816
                     /locus_tag="NOA01_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCI0633207.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            4943..5422
                     /locus_tag="NOA01_002025"
     CDS             4943..5422
                     /locus_tag="NOA01_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HCI0633208.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            5641..6321
                     /locus_tag="NOA01_002026"
     CDS             5641..6321
                     /locus_tag="NOA01_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCI0633209.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            6329..7660
                     /locus_tag="NOA01_002027"
     CDS             6329..7660
                     /locus_tag="NOA01_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCI0633210.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            7675..8637
                     /locus_tag="NOA01_002028"
     CDS             7675..8637
                     /locus_tag="NOA01_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HCI0633211.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            8748..9086
                     /locus_tag="NOA01_002029"
     CDS             8748..9086
                     /locus_tag="NOA01_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HCI0633212.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            9165..9872
                     /locus_tag="NOA01_002030"
     CDS             9165..9872
                     /locus_tag="NOA01_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HCI0633213.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(9975..>10590)
                     /locus_tag="NOA01_002031"
     CDS             complement(9975..>10590)
                     /locus_tag="NOA01_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HCI0633214.1"
                     /translation="STSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVF
                     FELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLI
                     QHLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQH
                     EKISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
BASE COUNT         3392 a         1883 c         2250 g         3065 t
ORIGIN      
        1 acagctctaa ctatcttttt tagaaaacga gcgttctgtt tgtctgtatc tgttgttgat
       61 gttaccctga aggtagatca ataaacaaac taagaggagg aagcacaatg gcagatttaa
      121 aaggacgttc taaagatatg aaagataaag tagtcggtga aggaaaagaa gcatacggca
      181 aagcaacaga cgacaagagc aaagaaacag aagggaaagc acaatcacta ggtgctgaca
      241 tcaaagaaaa agctcgaaat ttaggcgacg aagtcaaaga caagttttct cgaaaagacg
      301 aaaaaaaata aaaaccactt attactaatt gaatcaagca aataaaaaat ccgaaccata
      361 tctgcgagac agatatggtt cggatttttt atttgatcat tatcctgttt tttaaacttc
      421 gattttttga aattttttat tttttagttc aaagacttca tcacagcttt ttgctacttc
      481 aatctcatga gtcacgatga taatacagcg attttgttca tgggcgattt cttggaaaag
      541 tttgacgata tcttgtgaag ttgtttcatc tagatttcca gtaggctcat ccgctacgat
      601 tagttgatgt tcacagcaga tagcacgaac gatagcgact cgctgctgct gacctcccga
      661 aagtttggtc acctgttttt ttgctaatat ttcatcaatt cctacacgag ccagtcgatc
      721 taaagcaact tgtcgataat tctcttgctt ggtcttagtg atttccattg ctgctatgac
      781 gttgtctaga gccgataaat aaggaagcaa gttgtaagct tggaaaataa tcgagacatc
      841 atttttacga taacttctta acccgatttt gttcagtgaa cgatcttgat agtatacctt
      901 tccttctttt ggtgcatcta gtccagaaat aagcgaaaga aaagtcgttt ttccagaacc
      961 acttgctcct aaaatagcgt acatttttcc agcttcgaaa ttcagttcta catctttata
     1021 aagcgcatct tgcggatctt gataccaaaa tcccaattgt tctgtccgta acatcaaatt
     1081 cctcctttag tttaatagga tttttcttgg gtttaatctt aatatcccta tagaagctaa
     1141 aaagacagaa aacagactga ttccaaatgc tactccggct aatagtccca attgtgcagg
     1201 tttgacattg atttccaatt cgctgacttg atcagaaaca gcaaatggat tattcgtggt
     1261 tccttgttga ccatcgcgtt gaccgccttg ttcttgtctg ttacctggca tttgtccacc
     1321 gccaggagct tgctggtttt gtgtcgtggt ttctgtctgt tggtcaagta gctgctgtcc
     1381 cacggcattc gcgacgacgt ttccgctaag cgaagcaact cctaatgcaa gaatcataca
     1441 aatagccact tcggtaaaca attggagaat gattttactt ctggattctc ctaaagatag
     1501 caagacgcct aactcatgcc gacgctcccg aatggtaatc ataatgatca gagtaagtat
     1561 gataataccg gctacagcca ctagtaaaac aacattttta gaaaaacttg ccacgttgtt
     1621 taaaggttcc aacattgact gatacatgga atcatttgat tgtaagctaa atgtatctgt
     1681 gtcgatcagc ttctcagctt ctttgagaaa ctcatccatt ttatctggat cagatagagt
     1741 gtaaatagca gaatctatcg tgttatcgtc agatgtccca tttagttcat ttgcaaaagt
     1801 gtaagaagtg taaagtgtat tcgacggatt catgaagttg aatttcatac ccatactgcc
     1861 gcttgtttcg ctggattcat aaattccttt gatcgtcatt tcataagtgg tatcttcatc
     1921 ttcagtactc gtgattgtga aagtatctcc gactgccagg tcatttgttt ctgctagagt
     1981 agaatcaatg actacattgt ttgtgccttt gtcgctgtca tctattcctt caccatccgt
     2041 gatttttgct gttccatcag aaaaattact attttgtgca gttgctgaca caccactgat
     2101 ttgaaagtct gcttgagtca tttgcggatt cccgcccatt cctccagcag gttcaccgcc
     2161 tgcatcagta gtttctgaac tgtcttcact tgaatcagaa ctagtgatgg cagttattcc
     2221 atcttttgcc atagctgagg cggaactttc aaaggaataa ctctttacat tatctaattc
     2281 agctattttt tccgcgtccg ttaaggaaac tggtgtaaga gaaaaactgc ctggatctgg
     2341 acgttcttca gtggtagaac cgcttgaaga agtttgttcc tgttttttaa atgaagcttc
     2401 tcgattggca cttaaagtca ctgttgctcc aacttctttt tgtgcttgat tcgctgcttc
     2461 ttctgcagcg cttcgaatcg tgaggccagc tagcacaaaa ataagaatgg ctgtgaacac
     2521 ggcaaacaat aacaatgaac gtccccattt tacttttgta cttttccatg cacgtttcat
     2581 aaaattcata ttttgcatcc ctcctagtag atgcatttga taatagacaa cctatcttaa
     2641 agctagctta aatcttatgc tgtttgcaca ccatcaacat gaggttctgt atatataaaa
     2701 agtcactata gttattgcaa aaaatgtggt aaaatgaaaa aagtatgttt caaaccgacg
     2761 aagaagaaat gaaagaggaa tagcaaaaga aatcctagat gctacagagg gatttttacg
     2821 tgcatagttg ttctaacaat tgctgatact agcgatttag tttatttgtt gtataataat
     2881 ggggaacagc tgaatactta tataagtatt ctggctatag cgtttcgcaa aagatccatt
     2941 ttgttaatga agtatatcca aaataaaatg cccatccaac tgtcagaacg cggatgtgct
     3001 ggtaattttc ctgctgattc tccataagtt gagaaatagt gattttgcat atgcttcatt
     3061 ggaatggctc atatatttag ataaagaaga aaatcgacat ctttaaactt ggatgtcata
     3121 agaagaggtg aagtttgtga gtaccattaa aattatccca ttaggcggcg ttcgtgaaaa
     3181 tggaaaaaac atgtatgtcg cagaagtggg cgaggatatt tttattttag attgtgggct
     3241 aaaatatcct gaaaatgagt tgttggggat cgacgtcgta atccctgatt ttacgtattt
     3301 agaagaaaac attgatcgtg tagctggtgt ctttttaaca catggacatg cagatgcgat
     3361 cggggcactt ccttacttgt tgtcaaaaat ttctgtacca gtattcggga cagagttgac
     3421 gatcgagtta gcaaaattga atgtcagtcg taatgaagca gccaaaggat ttaaggattt
     3481 ccatgtggta gatgagcata cagaaataga ctttggtgaa acagtagtga gctttttccg
     3541 taccactcat acgataccag attcagtggg gatcaatctg aaaacaaaag aaggtagtat
     3601 cgtttatacg ggagatttta aatttgacca aacggctatc ccaatgtatc aaacagatta
     3661 tgcacgattg gcggaaatcg gcaaagaagg agtcttagcc ttactaagtg aatcggcaaa
     3721 tgcagaaaat cctcagcctg tagcttctga agcccaaata gcagatgaag tatttgatac
     3781 gatcaaatat tgggaagggc gtatcatcgt cgcttgtgta gcaagtaatt tgcaacgagt
     3841 gcaacaagta ttggatgcag cagaaaaagc acaacgaaaa gttgtcttga ctggtcaaga
     3901 tttcgaacga attattcgca cagcgatgcg tttagaaaaa cttcaattac caagtgaaga
     3961 tctgcttgtt acgttgaaag acatgaaaaa ctatgatcca gaagaactga tcattttaga
     4021 aactggccga atgggtgaac ccatcaagtc tctgcaaaaa atggcaaacg gtacacaccg
     4081 taatgtcaag attgaagaag gagatttggt ttatatcact actacgccaa gcatcgcgat
     4141 ggaaaccatc gtggcgaaaa cggaagatat catttatcga gctggtggaa cagtgaagtt
     4201 gatctctgag aatatgcgcg tttctggcca tgccaatcct aatgacttgc aattaatgat
     4261 caatttgatg aaaccaacat attttgtgcc agttcaagga gagtatcgtg aattagctgc
     4321 tcatgcagat ttggctcatg cagtagggat gccatacaaa aatatctata ttactggacg
     4381 cggagacgtg ctggaatata agaacaagcg tatgagtgtt gcaggaacta caagtgcaga
     4441 aaatgtaatg attgatggat taggcatagg tgatatcgga aacattgttt tacgtgatcg
     4501 aaaaatcttg tcagaagacg ggattttcgt agcggttgtc actatcagtc ggcgagaaaa
     4561 gaaaatcatt tcaaaacctc aaattacttc acggggattc gtttacgtaa aagctagccg
     4621 tgatctgatg aaagaaagca gcaatatgat tgaaacaatc gttgagaaac atttagaaag
     4681 caatgatttt gagtggagca aattaaagca agacatccga gatcaattag gccgttactt
     4741 atttgaacaa acaaaacgcc gcccagtgat cttgccagtc attatggaag ctactcaacg
     4801 tcgaaataaa aaataaacaa aaagagtaag atgaacctct ctatagagtg ctcgtcttgc
     4861 tctttttttg cgaaaaacaa ataatttttg aatacagcat ccctcgcttt ataataaacg
     4921 atagaataaa gggagtgaat caatgatgag tcaaacaaaa gtcgaacgtt atttagaaga
     4981 acaaaagatc agctatcaac cacttgattt tagtcatgtg gtagaagaat ctttcacaga
     5041 agaactacaa aaaagaggga ttgatccagc tttgatatgt aaaactctcg tagtgaaagg
     5101 aaacaaaact ggggtgatca tcgcattgat tcctttgaac gatcacttag attacaaaaa
     5161 aacgagacaa gtgacgaaag atcgtaaagt tggttttcct ggaatggatt ttgtattaat
     5221 gcatacagga tatccccacg gagcaaacac ccctattgga ataaaactcg cacatcctga
     5281 ttattgtttt ttagcagaca gttctttaaa agaaaaagaa gaagtaattg tgtcaagcgg
     5341 tgaaatcggg agaagccttc ggataaaagt agccgattta gaaaagatcg tccatcctat
     5401 ttacgctgat ttaattagtt aagggacgag aagagaagct aagcagagca gagacctcaa
     5461 acttgagatt atatctgctt ggtacttggt gcatcctgta ttatctgtta caattgcttt
     5521 gtacatactg tactatcaat ccaatgaaaa tatatttagc agctatattt tttcattgat
     5581 gtgcaagtga tgaaatcgtt tttaataaaa aagatacaga gaaaacgcga ggtgtaaaat
     5641 atgaaaatta cgtatcatgg acattcctgt ttaacaattg aaatggaaaa tggccaacgc
     5701 gtgatgattg acccgtttat tacaggaaat ccattgacag acttaactgc agaagaagta
     5761 aagacagatt ggttgctgat tactcatggt catagtgacc acataggaga tatgattcct
     5821 attgcaaaag aaaatcaagc cacaattatc agtattgttg agattgctga ttatgcgaac
     5881 agcagaggag tagattcatt cggtatgaat atcggtggaa aacatgcgtt tccattcggc
     5941 gtggtaaaat tcgtacatgc acagcatagt tcaagctatg aagtagacgg tatcgttcag
     6001 tatatgggcg agcctagcgg aattattatt caagcagaag gaaaaacgat ttatcatgca
     6061 ggggatactg cttatttcag tgatctagga ttgcttgcag aagaattcga tattgatgtc
     6121 gctttcttgc cgattggcga taactatacg atggggccag aagatgcagc aaaggctgcg
     6181 caaacaattc gtgccaagaa agtagttcca atccattaca atactttccc tgtcattgaa
     6241 caagatcccg aatcttttgt agagttactg gaagatagtg aaggaaaaat aatgagcgtc
     6301 tcggaaacgc tggaagtgta ggcagaaaat ggcgacaaaa catgatttga ttttagaata
     6361 tattgaaagt ttacctgttg gcaatcgaat ttctgtaaga agcatcgcaa aagaattgaa
     6421 tgtcagtgaa ggaacagcgt accgtgcaat caaagatgca gaaaatgtcg gactagtttc
     6481 aacaattcaa cgagtaggga ccatccgtat cgaacgcaaa ttaaaaaaac atattgaacg
     6541 tttgacattt ggcgaggtcg tcaggatcat tgaaggggac gttttaggcg gggcggcagg
     6601 attagacaag gtcttgaata aattcgtcat cggcgcgatg actaaacatg ccatggaacg
     6661 ctatattacg ccaggatcac taatgatcgt cggcaaccgg gcggaagtcc aaaaattagc
     6721 attggaagat ggcgccgcag tattgatcac aggaggattc aatacgactg ctgagattgc
     6781 aaagctagct gatgatcttg aaatgccgat cttacgcacc tcttatgata catttacagt
     6841 cgcaaccatg atcaaccgag cgttaagcga ccaattgatc aaaaaagata tcatgcttgt
     6901 cagtgatatc tatatgccag ctgaaaagac tcattatttg cagcagacag atacgatttc
     6961 tgattatcag cgtttgtcag aagaaacgca gcattcgcgt tttccagtcg tcaaccgcca
     7021 tcatcgtttg atggggattg ttacggcaaa agatgtttta gggaaatcgc caaaccagat
     7081 catcgatcgg gtgatgacga aagaaccaat cagtgtaaag aaaacaatga gtattgcctc
     7141 tgttagccat caaatgatct gggatgggct agaagtcatg ccggtcgttt ccgatgactt
     7201 gacattggaa ggcttgatta cccggcaaga tgtaatgaaa gcgatgcagc ttgtccagcg
     7261 acaaccgcag attgcagata cgatttcgga tcagatatct ggaaatatcc atacgattga
     7321 tactgaccgt gaaggaaatc gtttggaaaa ccctaagttc aaattcgtag tagcaccaca
     7381 aatggtcaat ggcgttggaa ccatttcttt tggcgtactt agcgaaatca tttctgatgt
     7441 cgcacaaaaa acaatggtga tgaaccaaaa acgaaatatc ttgattgaac aagtgaacct
     7501 acattatttg cggctgatcc aattggaaag cgaattagac attcgtccac gtgtattgga
     7561 aatcggcaga cgttccgcta aactggatat cgaagtgttt attgaaaaca ccatcgtttc
     7621 caaagcaatc gttgtttgtc aagtaatgga acgttcttag aaagcaggag tgaaatgaca
     7681 gtacagcaag aaatagtaga tacaataaaa aaatacgata aaatcattat ccacaggcat
     7741 ttgcgccctg atcctgatgc attaggatca caagtaggat tagccgaact attacgggaa
     7801 agtttcccag aaaaagaaat acgtcaagta ggtgaaacga ttgaagggct tcgcttttta
     7861 gcagaaatgc aagaagttga agatgtcttc taccaaggag cgctagtcat cgtgactgat
     7921 acagctaatt cacccagaat cagtgatcaa agatatcagt tggcagaaaa actgatcaaa
     7981 atcgaccatc atcccaatga tgacccttat ggagatcttg tgtgggtaaa tacaaaagca
     8041 agtagctgta gtgagatgat tgcagaattt gctttgatgt ttcctgaatc tttaaagatg
     8101 aacgttaagg ctgcacggtt actttatgca ggaatcgtcg gagatacagg gcgctttttg
     8161 tatccctcca cgacgtcccg tacattagag attgcttcga tattgagaaa ttatccattt
     8221 gatgcaagtg ctttgaacag agagattgaa caaatgccga tgaaagtcgc taaactttcc
     8281 ggttatcttt atcaaaatat ccaggtggat gaaaatggtg caggaagaat aatccttccg
     8341 caagaaattc ttgataaata tggaatcgat gattcagaga cagcttcgat cgtttcactt
     8401 cctggagtga ttgacgaagt gctggcttgg gggatttttg ttcagcagcc ttcagggtat
     8461 taccgcgtac gtttacgttc taaggggccg atcatcaatg aattggctaa acgccatcat
     8521 ggaggaggcc atccattagc aagtggtgcg aatgcaaaag atttagcaga agtagaggaa
     8581 atctataaag agatccaaga tatatgccaa tcgtataaga gcagaaaaac agaatgatac
     8641 ttcgttcgaa aacgtgcctt ttgtagtaaa aatcacaaat gtatcttttc aagatcttct
     8701 ttttccgtta caatagtgct cactaaagat tttaaaggag aaagaagatg acattaccaa
     8761 attgcccaga atgtggctca gagtacacat atgaagatcg aggcttgttg atttgcccag
     8821 aatgcgggaa cgaatggaac aaagaagaac aagaaactgt catagaagac ggattagtgg
     8881 tattagatgc aaatggaaac cgtctgttag agggcgatag cgtgacagtg attaaagact
     8941 tgaaagtaaa aggtgcgtca ggtgccatta aacaaggaac aaaagtcaag aacatccgtt
     9001 tagtagaagg cgatcataat atcgattgca aggtagaagg atttggaccg atgaaactga
     9061 aatcggaatt tgtgaaaaag aattaaagaa tattcaaggt cttttcacat tcttttccta
     9121 ctactaatga taaaataaag taaacgaaaa ggagtgaaac gaaaatgtat ggttatggaa
     9181 tgtatggttt tttagatccg acatttcttt tggtcattct tggattagtt atttccggaa
     9241 ttgcgtcagc gtacgtcaat agtactttcc gaaaatacga tcaagtgaga agcaaaaaca
     9301 atgtgactgg tacacaagca gcacaatata tattacaaag tcaacagata aataatgtcg
     9361 gtgttcaaca aattgccgga gatctcacag ataactacaa ttcaggtaat aagatgttga
     9421 gtttgtctca agcaactgca cagtctactt cagttgctgc gataggtgta gcagctcatg
     9481 aatgcggaca tgcggtacaa gatgcttcgg gttatgtccc attgaagatt cgtgcagcac
     9541 ttgttccagc agctaacttt ggttcgatga tttcgttccc cttgattatg gtaggtgtat
     9601 tgcttagctg gaactctacg ttaatcaata tcgggatttt cgctttttct ttagcattac
     9661 ttttccaatt ggtgacatta ccagtcgaat ttaacgcgtc tcggcgagca atccaaattt
     9721 tgagtgaagg cggactactg acggaagaag aagtaccgat ggcaagacac gtattatttg
     9781 cagcagcact gacttatgtt gcagcagcgc tttcgacatt cctgcagctg ttgcgcttga
     9841 tcttattgtt cggcggcaac cgaagagatt aaaataaaga agttgtgaaa tcagcgcttt
     9901 gatctgagct aataccgaga gttagctgat tgaataccat ttatttggaa taagaggatg
     9961 tgacaagaat tttgtcacat cctctttgtt ttgttctcat aacctttttt catatacagt
    10021 tcatgaaccg cttttattaa cttgccaaat aacactgtat tgtgcgttgt atccagatag
    10081 aaagtctccg tttcatctga aattttctca tgctgatctg taatgatcaa atcagcatgt
    10141 tctgaatatg cggtaatttt aacagcatct ttgcgaaata actgctgcaa atggctttga
    10201 agatgataat ttcctgtaaa ctgtttggtt atctgcaaat ggatgagtaa ctgctctttc
    10261 gtttctgttt gtagtaagtg ttggatcaat tgactaaggt attgactgat ttggtttctt
    10321 tccccatttt ttgaaaaaat tgtatcgatt ttttctctta atttcttttc ctgtataaaa
    10381 gaactagaat gattggacgt ggcttctgga aagcaaagtg tagaaaaact actgctggat
    10441 tcacccaaca gttcgaaaaa aacaactgcc aacacaagaa aataaaaata aattggtatt
    10501 ttattttcta aatggttgag aggaaactgt tgagttaagt aagtcatcaa ttgaccgact
    10561 tgtcgacaaa taaggttttt gcttgtggaa
//
LOCUS       DAIGOK010000096        10524 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.096, whole genome shotgun sequence.
ACCESSION   DAIGOK010000096 DAIGOK010000000
VERSION     DAIGOK010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.096"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            152..1048
                     /locus_tag="NOA01_002032"
     CDS             152..1048
                     /locus_tag="NOA01_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HCI0633215.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1198..2139)
                     /locus_tag="NOA01_002033"
     CDS             complement(1198..2139)
                     /locus_tag="NOA01_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCI0633216.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2265..3386
                     /locus_tag="NOA01_002034"
     CDS             2265..3386
                     /locus_tag="NOA01_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCI0633217.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3644..5146
                     /gene="eat(A)"
                     /locus_tag="NOA01_002035"
     CDS             3644..5146
                     /gene="eat(A)"
                     /locus_tag="NOA01_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HCI0633218.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5174..5740
                     /locus_tag="NOA01_002036"
     CDS             5174..5740
                     /locus_tag="NOA01_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCI0633219.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            5951..7012
                     /locus_tag="NOA01_002037"
     CDS             5951..7012
                     /locus_tag="NOA01_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCI0633220.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            7015..7911
                     /gene="murQ"
                     /locus_tag="NOA01_002038"
     CDS             7015..7911
                     /gene="murQ"
                     /locus_tag="NOA01_002038"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCI0633221.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7925..9907
                     /locus_tag="NOA01_002039"
     CDS             7925..9907
                     /locus_tag="NOA01_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCI0633222.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(9972..10418)
                     /gene="nrdI"
                     /locus_tag="NOA01_002040"
     CDS             complement(9972..10418)
                     /gene="nrdI"
                     /locus_tag="NOA01_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCI0633223.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
BASE COUNT         3436 a         1854 c         2278 g         2956 t
ORIGIN      
        1 taggcgaatt ttaatagaag aatgatttat ttcatcatat tttcatacaa atgttaaatt
       61 ttcatacaaa tataggaaac gttttactgg ctttttacta tttaaacagg ctatcatagg
      121 aaaaagtgac agagaaagtg aggcggaaaa gatgaacaaa aatgacgtac tgctagttgc
      181 aaaaaataaa tcactttatt ttttgccttc catccgctct ttagatgttt ggtccggaaa
      241 agatttacta cagtcggaat tcattaaatc agctagacaa cagttggcaa attatcagct
      301 gatcgttttt ttagattatg gatttgatat ggagatggct cgacgcgtgc gcccatttac
      361 caatgcaaaa attgtgttat ttttttggaa tcacttcaaa gcagaacacg aggcgatgct
      421 gaagcaggca caagatgaac cggcaatcga tgagatttat cattttgatt ttttggaagc
      481 aaaagaactt gggctgaaac ataatagttc attttattcg aaacatatga atttttcttc
      541 tagcgaacca aagacggatc tattttttgg tgcaacgaat aatgggagaa aagaacgagc
      601 agaaagctat aagcaggaat ttataaaaag agatgttacg accaactatt tcattcttcc
      661 acatcgaggg aatgaacaac ctggatatct tacttatgaa gagtacctga aaaaaacagg
      721 ggaatcgcgc gggattctgg aactgttgcg ggagggtcag caaggagtga ctttgcggac
      781 atttgaatcc atgtattttc aaaagaaact agtgacggat aatcaagcga ttgctcatta
      841 tcatttttat cgtcctgaaa atattttttt gcttcgagaa cgtgatttag atgaattacc
      901 ggaatttctg catacaccat ttaagccaat cctatcagag ctattggaat tttatgatgc
      961 agaaaattgg gcaaaacgat ttatcactaa cgattccact atttatgagc aatacgaata
     1021 tgacctctcc tctgttgaaa acaaataaaa aacctacatt ttgaagagat tatgtactga
     1081 aacaaacaag cacaattctt atggcatcat gagagttgtg ttttcttttt gatcttttat
     1141 tgggcttaaa aaaagagaac cagctgctga ttcagctgat tctcatcaga gagcctttta
     1201 ttctgcgcca aggattcctt cttttgcatg gactaattgg gtcaaaaagg cacaatatgg
     1261 tgtagcaata tcatattttt gtccttttcg ccaaatggca ccattgatgt agtcgatttc
     1321 agtcaaacga tggtttttga tcaggtcttg gtacatagat gggtaatgca acccgatgtt
     1381 gtcaggatca taagtagaag caatatgttg gtacacttct tcttgatcta gtatgattcc
     1441 ttcttttgct gcgatagaag caaattcaga tacgattgtt cgaaccatgc tctctgccgc
     1501 tttttgcgcg cctagttcag caatattaca atccaaaatc gtgcataaac cattcaatgt
     1561 tccatttaca caagcttttc gccaaatgga ataacgaacg ttatggctat agatcggatt
     1621 taaatttgct tcttgaaaga cttctacaac tttttttgta aatgcttcgc cttctggttc
     1681 caagttctct agttcgattt ctccatcacc taacaatgtg acttttccgg gaccagctag
     1741 accagctgtc cacatcgtga tcccaaataa gatatttttc tttggcacat atttttccag
     1801 tacatcttca tgacctaatc catttagtaa acacaaaaca taagtattgt cagtaataat
     1861 ggattggatc gaacaaaaca tatcatctaa ttgattggct tttgttaatg ctacgatcaa
     1921 gtccacgtgt tcgttgcttt cgatgatttc ttctggtgaa tagattggca gttttgcgac
     1981 cacttcttta ccgttaaagt cagcaatcaa cccatttgta cgaatggctt caatgtgagc
     2041 aggccaacga tcgatcaaga ggacctcatt tccgctttga tgtagcatca atccgattcg
     2101 gctgcccatt gctccggccc cagctattgc gattttcatg ttttttctct cctttattca
     2161 atgagttccc ttttagtata tagaaaaatg gaagaaaata gcattgtttt gtttgaaaaa
     2221 aatgaaaaaa agtgtcttac ttaagaagaa agtgagggat acagatggat attttggcgg
     2281 caattgattc atttaaagga agtgcttctt catttgaact aaatcaggca gttctaaatg
     2341 gctgggaaga tcgtaaagga agaaaaatca atgtgccgat tgcagatggc ggagaaaata
     2401 ctgcagaagc aatctattac gcgctcaggg gaacatggcg taccattgat gggatggatc
     2461 tcttattccg tcagcgaaaa atcagctatt tattgacaac ttggcaagga aaaaacaccg
     2521 cagtcatcga atctgcacaa gcattggggt tagatctact tgataaacca acggatcaaa
     2581 ctatccgact tgcttccagt tatgccttag gagaacttat taaagatgca ctgcttcaag
     2641 aagtagaaca aattattgtc actttaggag gaagcgggtg tagcgatggt ggtctaggat
     2701 tactccaatc cttgggtgct tctttagaag ggtacacaga aggaaatcct ttattatcta
     2761 ctaaaaaact tcattttgat cgaagaaacg ttcttgtttc taaagtaaaa ttgattatcg
     2821 ctgcagatgt taccaatcca tatacaggtc aacaaggagc tgcttggcaa tttggaagac
     2881 aaaaaggagg aatagatacg acgctagctt tcttagataa acaagcagga aagattgctc
     2941 agcaaataaa agatttctgg gaggtggatt tagatgatat tccaggaagt ggagcagcag
     3001 gcggtattgg tggagccctt tttttgttgg gagcgaagat ggtttctggt ttcgatttag
     3061 tcagccaaat cactggattg gaaaaaaaga tcgaacaagc agatgtcatc attacaggag
     3121 aagggcggat cgatcagcaa acgatccacg gaaaagttcc atacggtgta gcagtgcttg
     3181 caaaaaaata tcgaaaacca ataatcgcta tttgcggaag tagagaccaa gagctagaag
     3241 gactttctga ttttctccca attgtcttaa gtattcattt gggtccaata ggtttagaag
     3301 aagcaatgga acacaggcaa acattagaaa aaacaaaaat cttaggacag acacttagca
     3361 gatttttcac catcctcccg aagtagttga caaaatcatt ttctttatgc tatcgtaatt
     3421 tcaaatatag gacaaagtga gggatccgtg cattgaaaaa ttaagtcgat tgatgaaagc
     3481 tgcattcaga ctgtaccttg tggagacaag gaagcagtgt gcagattttc gggattggct
     3541 tggttttttc gatgtatggg tctttttgtg tgtatccaac ccttgcatcc tctttagcca
     3601 gttctgaaaa taggactggt ctttttttac gaggtgatgg aaaatgtcta aaatcgaaat
     3661 aaaaaatctg acattcggct acgacagcca aggcacatta ttatttgaac aagcaaatct
     3721 aaattttgac acacaatgga aactaggact tatcggacga aacggtcgag gaaagacaac
     3781 tttactgaat attctacaaa acaaactacc ttatcaaggg caagtaatcc atcagcaaga
     3841 atttgcctat ttcccgcaac agacaaaaga taaagaacgt ttaacctatt acgtgttaaa
     3901 tgatattacg gattttgaga tatgggaaat cgaaagagag ctccaattga tgcaaacaga
     3961 tcctgaaatc ttatggagag aattcagcac actatcgggg ggagagaaga caaaagtcct
     4021 actggcactt ttatttgtgg atgacactca tttcccgtta atcgatgaac caacgaatca
     4081 tttggatatc tctggtagaa aacaagtagc ggcttatttg aaaaagaaaa aacaaggctt
     4141 catcgtggtc agccatgacc ggggatttat cgatgaagta gtggaccatg ttttagcaat
     4201 cgaaaaaagt caactggaac tttatcaagg gaatttctct atctatgaag aacagaaaaa
     4261 acttcgtgat gaatttgaaa tggctcaaaa tgaaaaattg aaaaaagaag tcagtaggct
     4321 aaagaaaaca gcagctgaaa aagccgaatg gtctcgttcc cgagaaggag ataaaacaaa
     4381 gaaacaagtc ggattcatcg atactgaatc tagacgagtg aataaaggag cagtgggtgc
     4441 tgatgctgca cggacgatga aacgatccaa agcaatcgtg aatcggatgg agacccagat
     4501 cagcgagaaa gaaaaactat taaaagatat cgaatatatc gattctttga cgatgaatag
     4561 ccaagcgtct caccataagc gacttttaag cgtagaagat cttcaattag ggtatgaaaa
     4621 tctgttattc gagccaattc attttacaat cgagcctcat cagcgggtgg cgatttcagg
     4681 tcctaacggt gcaggaaagt catccattat ccattatctt ctgggggcat tcaacggcaa
     4741 ggttatagga gaaaaatacc agccaaaaca tctgagcatt agttatgcaa gccaaaatta
     4801 tgaagacaat cgaggaacgt tggcggaatt tgcagagaaa aaccaagtag actaccaagc
     4861 atttttgaac aacctccgaa agcttgggat ggaaagagat gtttttcata acaagatcga
     4921 gcagatgagt atgggccaac ggaaaaaagt ggaattggct aaatctttat cacagccagc
     4981 tgaactatat acatgggatg aaccattgaa ttatttggat gtcttcaatc aagaacaatt
     5041 agaacaactg atcttgaacg tgaaacctgc catgttacta gtggaacatg atcaaacctt
     5101 cctggataaa gtatctactg agattatttc tcttgagaga atctaaccct tgtaatctca
     5161 agaacaacaa ggtatggtta aggggaactt tgccaacgaa ggagagaatc acatgcttta
     5221 tgtaacgaga cacggagaaa caacttggaa cgcacaagga ttagtttgcg gacgggcaga
     5281 tgtgccgctg actgaaaaag gacagatgca ggcacagaaa ctagcagaaa aggtagtaga
     5341 tttgcctgtt ccaatcacga aaatcatcca ttcccctctt caacgagcaa gagatacagc
     5401 acaagcagtt gctgatcgtc tcagcttgcc gctgaccgtt gatgaacgat tggtagaaat
     5461 ggattttggg gattatgatg gactgccaag caaagatgag aactttcaaa aagcacgttt
     5521 agcgttcgct gtacgattcc caaatgggga gtcagtctta gatgtgtatg cacggatcgt
     5581 tcctttattg aaagaatgca tcgaagacga agaaaatgtc tatctgttag tttgccataa
     5641 tgccttgata agagtgatca atgcttattt tcatccgatg cctaatgagg ggttctttac
     5701 attcatggta gataatacag aattggtttc ttatgagtaa ttatgttttt gtgttattta
     5761 aataaaatat ttcaaggcac tgcttttgct tagcagtgcc ttttttaggt tatgacaatt
     5821 cttttgataa tataaactca acctttagaa atgaaattta attattttaa taaaagtatt
     5881 gaaattttgt ttctttagat atatactcta gtcatagaaa tgaagacgga tacagaaaaa
     5941 gagggataat atgtttggct tttcaatatt tatgaacgat tctttgtcag aagagaagaa
     6001 acagtatatc cgccaaatgg catccaatgg ctttgtcggt attttcactt ccatgcatat
     6061 accagaagat gatgtttcgg catatcaaaa gcgattgatc gaactaggaa gcgaagcgaa
     6121 aaagaataaa ctagaattga tggtcgatat ttcaggagag gcattggaac gagcgggttt
     6181 ttcattagaa gatctaacgc ctttgaaagc aattggtgta acaggtttaa gaatggatta
     6241 tcatatttcc aatcagcaga ttgccgactg gtcacatcaa ttgaaaatca gtttgaatgc
     6301 tagcacgatc acgcctaaag atatcgatga attgaaagaa gcagaagctg atttctcaca
     6361 gattgaagct tggcataatt attatccacg tcccgaaaca gggctggaca aagaatggta
     6421 tcagaaaaaa aatcaatggc tcaaaattca aggtctcctt gtccaaggtt ttgtaccagg
     6481 agatacagag ttgagaggcc cactatatca aggattgcca actttggaag aacatcgagg
     6541 gatccatcct cttgcaggcg ctctagatct attagcaagt aacacggata ttgtttacat
     6601 cggagatgca ggtctgtcag aaaacgttca ggagcaattt gcttctttcc aaaaagaaca
     6661 gacagtcttg ctccatacgg aacctgtaga tgaagaattt tacgaatata tattagggaa
     6721 gcatacgaat cggcaagacg atgcaagaga tgtcatacga agcgtagatg cacgttttcg
     6781 agagattccg cctatcccag caagaaatac agctacgcgt atgaaagggt ctattacttt
     6841 agataacgaa aagtatttgc gctatatggg tgagatccaa ttaaccaaat atgatttgcc
     6901 agcggatgaa aaagtgaatg tagtagctaa agttatcaaa gaagagctac cattgatcaa
     6961 ccaaatcaaa gctggtatga actatcaatt tataagaaaa gaaggaagat aaaaatgagc
     7021 aaaatcaatt tagagaacct aacaacggaa cgcagaaacc aagaaacttt tggattagat
     7081 gagatgagta tttctcaagc cgttcaacta atgaacaaag aagatcagaa agtggccgct
     7141 gcggttgaaa aagaactgac aagtatcgaa ccagtgattg ctaacacgat tgagtccttt
     7201 aaaaaaggtg gacgtctgat ttatatggga gccggaacaa gtggaagact aggtgtgtta
     7261 gatgctgctg aatgcgtacc gacattcggt gtagaaccgg aaatggtcat aggtctaatt
     7321 gctggtggag aagaagcaat gactgtagct gtcgaagggg ccgaagattc ttatgagcta
     7381 gggcaagaag atctaatcaa gctgaatttg acaaaaaatg atatagttat tggtatcgca
     7441 gctagcggtc gtacaccata tgtgatcggt ggattagatt acgcaaaatc gatcggtgct
     7501 gtcacaggaa cgatttcttg caataaacaa gccgaaatca gtaaacacgc ggatttccca
     7561 atcgaagtcg atgcaggtcc agaatttttg acaggctcta ctcgattgaa gtcaggaaca
     7621 gcacaaaaac ttatcttgaa tatgatttct actatcagta tgattggtat aggtaaagta
     7681 ttcaataact tgatggtcga tgtcaaacca acaaatgaaa aactaatcga acgctcaaaa
     7741 cgaatcatca tgcaagcgac agaagctgat tacgaaacag ctgaaaagta ctttgaagaa
     7801 gcagatcaaa atgttaagct ggctatcgtc atgattttga caaatagtga taaagaggaa
     7861 gctcaagcaa aattagctga agcaaacgga tttgtaaaag ggaccttatg agggggaaaa
     7921 gaccatggca aaaaacgaac tgactttgga agaactggca aaacaaattt ataaaggggc
     7981 aggcggcatg ggcaatgtag aaagcattgt ccactgcatg actcgtgtgc gtatgaagat
     8041 catcaatgat gatcttgtaa atgtctctga attaaaagcg attccaggag tgcttggtgt
     8101 agtagaagat gaacagcttc aagtcatcat cggacctggg aaagtgaaca aagtagcaca
     8161 gcaaatggtt gatttggcag gcgtgaaatt aggagaaaaa ctttcttctg taagtacagc
     8221 tacagcaaat tcatcagcat ctggaaaaga ccgtgtaaac gaacgggcac aagaaatgaa
     8281 agcggcacaa aaatcgaaac aaaaaccatc taaaatcaaa tcggtattaa aagacatttc
     8341 taatatcttt gtgccaatga ttcctgcatt tgtcggtact gggatcgtgg cagggattgc
     8401 cgcagtttta gcaaacttag tcacagccgg aacacttgat gcagcaacat ggcaacaata
     8461 tatcgatgtg atgaacatct tgaaaaacgg gatgtttagc tacctagtta tctatacagg
     8521 tatcaatgct gcacaagttt ttggtgcaac accaactctt ggtggagtta tcggggccgt
     8581 tgtgatgctg acagggatga atccggaagc accactgaaa aacttattta ctggtgaagc
     8641 tcttgcagct ggacaaggtg ggatcctagg tgtcatcttc gctgtttggc tattgtcaat
     8701 cgttgagaaa aaactgcata aagtagtacc tgatgcggta gatatcatcg ttacaccaac
     8761 tctttcattg attgccatcg gtctgattga aattttcttc atcatgcctt tagcaggttt
     8821 catttctgat ggcatggtcg gtgggatcaa ctgggtactt ggcgttggtg gagcatttgc
     8881 cggcttcgtt ctaggcacat tattcttgcc gatggttatg ttcggactgc atcaaatttt
     8941 gactccaatc catgtgcaaa tgattgatga aactggtcgt acgctattgc ttccaattct
     9001 tgcgatggct ggtgcaggac aagtgggtgc agcattggca ttatggattc gctgcaaaaa
     9061 agataaagaa ttaacagaaa tgatcaaagg agctttacca gtcgggatcc taggaatcgg
     9121 cgaaccattg atttacggag taacattacc tctaggacgc ccatttatta ctgcttgtat
     9181 cggtggaggt atcggcgggg cagtgatcgg aatgatcgga aatgtcgggg ctatcgctat
     9241 tggaccttca ggagccgctt tgatcccatt gatctctgac ggtaaatggt taggctacat
     9301 ccttggatta ttggcagctt atgctggtgg cttcgttgct acattcttct tcggtattcc
     9361 taaagaccgt ttagaaaaag aagaattagc gactgaaaca gccacagtaa atgaagtgcc
     9421 tgaaaaagta gtacctcaaa cttcagctga aagtgtgact atcctctcat cagttgctga
     9481 cggaacagtt gtttctttag ataaagcaag tgatccagtt tttgcacaaa agatgatggg
     9541 tgaaggctac tttgtagaac cagaaaacgg acatatctat tctccagtta ctggggtagt
     9601 cagctctgtc ttcccaacga aacacgctat tgggatcaca acgccaaatg gtttagaaat
     9661 cttgcttcat atgggaatca acacagttga cttaggcggt acgccatttg atgtgaaagt
     9721 aacagaaggg cagcaagtga cacctgatac gcttgtagca gaagctgact tagaagcgat
     9781 caaagcagca ggcaaagaaa catccatgat cgtcttggta accaatatgg atcgtgtaaa
     9841 aaactttgtc ttggaaaaaa caggtaaagc aaaagcaaaa gcacctgtga tggatgtcaa
     9901 agcttaatat gatagaaaca atagaaacga aagaagaaac tgaaatctat ttttcagctt
     9961 cttctttttt attatttatt gttttctgct aaaatctgat aaattcgtgt cacatcagat
    10021 ggtgttcccc gtaattcata atctgccaag aaaggaaagc caaatgtttg tgcatattgt
    10081 ttggctgtta gacaatactg attattgaag tttttatttc cgctaccgac tacaccaaga
    10141 caaagtttat gattgtcttc atgttccaga tattcacgca tcgtttcggt caagatttct
    10201 gtatcaccgt tatctaaacc atttccgccg tctaaataag taggaacgaa agtaaaaaat
    10261 ggttctgttt ctttcgtaaa atcactattt tcatggattt cttttaaagt aacttcagga
    10321 ttgacaggat cttctgcatg cattttttca gcatattctg ctagatgttt ggcaaaggca
    10381 cgcgtatttc ctgaaatcga tatatataaa atgttcatgg atcatcctcc aattctttcc
    10441 ttttagtata gcatcttttt taagtttttt ggtggtgtaa tttggaaagg gactaggcgc
    10501 tttgcttgta taatgagaaa aaag
//
LOCUS       DAIGOK010000097        10354 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.098, whole genome shotgun sequence.
ACCESSION   DAIGOK010000097 DAIGOK010000000
VERSION     DAIGOK010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.098"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            5..304
                     /locus_tag="NOA01_002041"
     CDS             5..304
                     /locus_tag="NOA01_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCI0633224.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            377..1738
                     /locus_tag="NOA01_002042"
     CDS             377..1738
                     /locus_tag="NOA01_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCI0633225.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1760..2020
                     /locus_tag="NOA01_002043"
     CDS             1760..2020
                     /locus_tag="NOA01_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633226.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2034..2786
                     /locus_tag="NOA01_002044"
     CDS             2034..2786
                     /locus_tag="NOA01_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HCI0633227.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2880..3485)
                     /locus_tag="NOA01_002045"
     CDS             complement(2880..3485)
                     /locus_tag="NOA01_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCI0633228.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3538..4512)
                     /locus_tag="NOA01_002046"
     CDS             complement(3538..4512)
                     /locus_tag="NOA01_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HCI0633229.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4890..5609)
                     /locus_tag="NOA01_002047"
     CDS             complement(4890..5609)
                     /locus_tag="NOA01_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCI0633230.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5736..7007)
                     /gene="celB"
                     /locus_tag="NOA01_002048"
     CDS             complement(5736..7007)
                     /gene="celB"
                     /locus_tag="NOA01_002048"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HCI0633231.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7160..8557
                     /locus_tag="NOA01_002049"
     CDS             7160..8557
                     /locus_tag="NOA01_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCI0633232.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8638..9078
                     /locus_tag="NOA01_002050"
     CDS             8638..9078
                     /locus_tag="NOA01_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCI0633233.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9226..9552
                     /locus_tag="NOA01_002051"
     CDS             9226..9552
                     /locus_tag="NOA01_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCI0633234.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9553..10302
                     /locus_tag="NOA01_002052"
     CDS             9553..10302
                     /locus_tag="NOA01_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633235.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
BASE COUNT         3285 a         1826 c         2146 g         3097 t
ORIGIN      
        1 aaaaatgaaa aatcggttaa ttgtcgcatg tggaagtgga gtagcaacga gtcagacaat
       61 cgctagtaag atccaaagta tgttagaaga tgacggcatc gcatttcctg tagaagcagt
      121 tgattacaaa tcgattcaaa atgagcttct aactgctgga atctatgtgt atgtcgcaca
      181 acctgatgag gaagtacttg aacaagcaaa agatttgggt attgaagtat ttccaggaat
      241 tccattttta actggaatgg gtgtagaacc tatttatgac agtatcaaag agttagtaca
      301 gtaaaggagg attgttggat aaaaaagttg gttcatagtt ttatttacta tatttagaaa
      361 gaaagaaggt ttatttatgg aagttttcca aaccattatc aattatgttt tgaacttagg
      421 atcagccatt tttgttcccc tgattatttt gatacttgga ttgttggcag ggatgaaatt
      481 aaaaaaagca ttcatgtctg ctttgacttt agggattgct tttagcggaa tgtctatggt
      541 cattggctat atgtctaacg cagtaagtcc agcatcagaa gcattggcta aaaatacggg
      601 aatcagtttg cctgcacttg atcttgggtg gacaggtgca gcaagcatca catggtcgtg
      661 gagttatgct tttatatttt ttgcagtaac gattggggtc aattttgtac tgttactttt
      721 taattggacg aagacgttaa atgttgatat gtggaatgtt tgggggaaag ctttaactgc
      781 ttatttagtt tattatgttt ccggtagttt ggctgcaggc tttcttactg caatggtcca
      841 agttatattg gaattaaagc ttggtgatat gtttcaaaaa catatacaag atctcacagg
      901 gattccatta gtaacagtga ctcattttat gacatctgcc gctgtcttgt tgctgccttt
      961 caatatgatc atggataaaa ttcctgcttt aaataaacga gcggatacga atgcattgaa
     1021 gaagaaaatt gggatcttct ctgaaaactc tgtaatggga ttcattattg gattggggct
     1081 tggctttgct gctgcgtatg gcgtttcagg ttctcttaat ctagcgatcc aaacagctac
     1141 agcattaagt ttgtttccta tgatctctaa aatgttcatg caatctttgt ctcctctagc
     1201 agacgctatg tcagaattta tgaaaaagcg gtttaaggat cgtgaagtat atatcggatt
     1261 ggactggccg atattagctg gtcgttcaga aatttgggtt actgctattt tacttgttcc
     1321 tgtgtttatt ggttatgcca ttattttgcc aggaaaccaa gtattgccat tagcagggat
     1381 tatcaactat tcgattgcgg tcggcggatt acttttaaca ggaggaaact tggctcgtat
     1441 gctgatcttg ggaatcatca ctatgcctat ttatttgtac ggggccactt accttgcgcc
     1501 aatactcagt gatttagcag aaaaaagcgg agcagtcgag ggaatcaaga aaggacagtt
     1561 gatcacatgg tcatccatag aagggccgga gttcagggtt ttatttgcgg aggcatttaa
     1621 taggaatatt ctcgctattg taggatgcgt gatttttcta ggactgtttg ttctacttta
     1681 taaatatatg ataaaagcga aagtaccgag tgaacgttat gctgaactac aaaaatagca
     1741 ttgaaatggt gggaaagtaa tgaaaagaga agagacagat aaaataaaat ggacagtcgc
     1801 tctttgtggt acacttctgc tatttctata tggactcttt actcagaata tcatcatcaa
     1861 tctattggtc atttttttcg cactagtgat ctataaatat ggaaatcacg ttttattcag
     1921 agagtacgat gaaaaaagaa aacggaaaat agaagaatca atgaaaataa aagaagctac
     1981 caaagaaata ttgagagaaa aaagttttat aaaaagataa tggaggggtc attatgtcag
     2041 atggacgtat gttatttgag agagaacgac aggacatggc acgtatcgta agagtgattt
     2101 tcgaaaggaa aaacacaaat gtcgcaggag ggaatttttc ctttaaaacc actgatgatc
     2161 aagggaaaga ctacatcatc atgacgccga ccatgatgtc agaagcctat ctaggagaga
     2221 tttctcctgc acaggttcta gtagtagaac caagaacgcg tagaatcgtt gacggagtcg
     2281 gtcatttaac tcgtgaaatc aatatgcatg aagcgatgta tgagactaat ccagctatca
     2341 aagctgtcct acatgctcat gcgcctaatg caatgttctg ggcgactact gggttgccta
     2401 tgcctaattt gacagaagcc acagctaaag taaaagagat tcctgtcatg gactttgagc
     2461 ctaattgctc tgaagaactt gctgaattga caagcgacta tgtaaaaaaa cagcatctcg
     2521 aattaccgca tatgttgctg ctcaactctc atggcgtatt gatcaacaca ggtggagaat
     2581 caggaatcga agctatccat aaagcactgc aaatccttga tacagttgaa tggaatgcag
     2641 aaattgccta caagcagacg atttttcaag cattagggat tactgacggt ttttattcaa
     2701 aaggtgagaa aatcggggga ttggaagagt tgaaacataa aacggctatt tataataaat
     2761 ctgttattgc agctggtgga gactaaccta tctgacacaa aaaactacga ctttcacaaa
     2821 atctgatcca gatcgatatc tgcttcaggt tgtattatgt aagtcgtagt tcttttcttt
     2881 tatttagtta agaggtggtc tgtccattcc ctcgcttgct tgatttcgtc catcgtcaac
     2941 tgatgacctg ttacagttag ctgaatagtc acatttgctt ggcgtgtttc gaattgatcg
     3001 gccaattctt taaaggaagc aggggaaacg attggatcgt tatcaccata agaaatccag
     3061 acggttttat cctctaatgt ggaacggctt tgatgaacac ctagagacat cggatggaaa
     3121 aacaaccctt tatttagctc ggtctcacgc tccaataata aatgagcagc gatgtttgcg
     3181 ccatttgaat agccgacaag cagccagtca cttaacggta tcagttcttt ttcgctgatt
     3241 tctttgattt tttcatggag tttatctgtt tctttttcca aactgtccag gtcgaattga
     3301 ttcaatccat tacgcttgaa aaaacgattc atcccatctt cttggaccgt gccacgaaaa
     3361 gaaagcaatg tactgtttgg tgcaagaaag tgagcgatat ccagtaaaga atgttcatcg
     3421 ccacctgtgc catgaagtaa gatcaaatgc ttgccacttg ttcctttttc aaaaatgtag
     3481 gtcattttgt tccctctttt ctgtttctat agatgcaaat gaagtagcat gaagagatta
     3541 ttcacgtttt ttatttgcat cacttgtatc gaaaggacgg acataagctt caatggcttc
     3601 acgacgttct tctaagaatg gaggaagaga tagtttttct cctgctgatt ctgctggttc
     3661 atcttcaagg aatccaggac catcagttgc taattcaaac agtacgtgat gagcggctag
     3721 gaagtactca gattggaaat agaaacgatc gacgaaacca gaatttggca attcaatctt
     3781 attgattact tgaatccagt attccaatgc ttctttgtct gctacacgca atgctaagtg
     3841 atggatattt ccaaatcctt cgattgcttt tggcaagtct gtccgatgtt caataatgat
     3901 gcttgcacca tttccccctt caccgatttc aaataagtag aaatctcctg aatggtctgt
     3961 ttctttgaat cctaagattt tttcaagaat caagcggata tgctctaatt gactgacacg
     4021 gacaaagaca ggtccaagtc ctgtgatcgc aaactctgtc ggtacattgc tgtttttcca
     4081 aggaatgcca gctgctacac cttgattgtt ttcgtcagag atcaattgat aatactgatt
     4141 atcgaaatct tcaaattcga tatatttttt gccgaaacgt tcattgattg ctgaatgagt
     4201 aacgtcatat ttttcaaaac gttccaacca aaagtctaat gctgcgtcac ttggcacacg
     4261 gaaagaagca cgagagatcg tatctgttcc tttttttcct tttggctgat ttggaaaatc
     4321 aaagaaagtc atatctgttc ctggcgcacc agtatcgtct gtaaagtaaa gatggtaagt
     4381 ttcgatgtca tcttggttaa ctgttttttt gattaagcgc attcctaaaa tatctgtaaa
     4441 aaagcggtag atttttgtag cgctagatgt cattgctgtg acgtggtgaa ttcctctgat
     4501 ttgtgtatcc atgataaatt tcctcctgtt aacttacaaa aagtaagtag ttgatttaga
     4561 catagtatag agggtgcatc tctattttgc aattaatttg tcttctaaag tatcaggaag
     4621 gaatcaagaa tggaaaagag gagggacgga tcaataaaat aaaaagactt acgactagct
     4681 gtatcgattt caaaaaaact agctttaaaa agctaacttt agaatcatta cgggtggtca
     4741 taagtctttt tatgctgagt tattttttca tttttgagct aatgcttagg atagaataga
     4801 atgttgattt cacgatctct tcttgttact tgaaactgct taccactgtc acgatctctt
     4861 ggtaaacggc tttttcacaa cctctttggt taagttgaac gcacatccag cacagaatag
     4921 ttccgaacat caaatcggtt tcggctggaa gaatattcaa tcggctgtcc atctttgaga
     4981 tagacgactt gctgaacttc cagtacagga tcgttttctg cacagttcaa atattgctga
     5041 tcatattcat cacttttatc tgcagaaata gcacgataag cccctgcaaa atggacattc
     5101 aattcttgat gaagatgatc atagatagag gctaacaaat gttctttttt taaacccggc
     5161 acaagatgga ctggcataaa ggtatgctct aaaacatatg gctcgccatc taagatcctt
     5221 aaccggtgaa tattgtagac aggttgctcg gcatcgatca tcaatcgttc ttggatatat
     5281 tgattaggga attcaacatc aaaatgaatg atttgactct tcaaagaacg atgtctgcgt
     5341 ttcatttgga gactcaaacc ttcatactcg ttagcgggcg atgtgtcttt ttgcataaat
     5401 ggatgattca agattttcgt tcctgatcct cgctgcgaat agacaagtcc ttccattgct
     5461 aagatattga tggctttttt gattgtcatc cggctcgcac caaattcttt taccaagtcg
     5521 acttgattag gcaaaaaact atcgagagga tactcttttg aacggatacg ttctcttaaa
     5581 atatttgcga tttcttcgta ttttggcacg ttattcacct cttctagagt ataacgaaag
     5641 attatgcttc cgacaactcg aagtagcatc tggaacattg tttattcgaa aaaagaggtc
     5701 atgacaagtt tttgtcacaa ccccttaatg atctattaag caatatctaa atctggttgt
     5761 ttatctagta ctttcaggaa tgggtaccag ataaaggcaa cgatgacaaa gtcgatgact
     5821 tgcagcatac caccaagcca tgaatttgtt gctaagacac cgcttgcaat gattggcaca
     5881 gtccatggaa cagagacgcc agttggtgca gggacaagtc cagcagccat taccaaataa
     5941 ttgaacgtcg ttacgattag aggtgccaca acccaaggaa taaggattgt tgcattcaat
     6001 acgatcggta gaccgaagat cgctggttca tttacattga aaataccagg tgctaaagct
     6061 aatcgtccaa catcacgata ctgtttcttt ttaagaacaa aggccatcaa taaaacgaca
     6121 gctaaagtca ttccagagcc accaatacca acagtgaagg tttccatgaa tggttttgtg
     6181 atgatgtgtg gtaattcttt tccatgttga taagcttcta agttatcaag catcagtgta
     6241 ttccaaatcg ggtccattac agagttgacg atgatttgtc cgtgaagtcc gaagaaccat
     6301 aagaattgga caaagaacaa agcaatcaat gttgcaggta aactacttcc caaaccagtt
     6361 aatggttttt ggatcacttc ataaacgacg tcatgtaagt tagcattgaa aataccagtc
     6421 atcacagcat tgattactaa gaaaatagtt agtgtcacta cagcagggat caaagcggca
     6481 aatgattttg ttactgcagg cggaactcca tcaggcattt tgatctgcca gccgcgtttc
     6541 gtgattctgc agtagatttc tccagccaag aaagcagcga tcattccaat gaacattcct
     6601 tttgcgccaa gacgatcaag tgaaagcaca ccagtcacat cttcaccgcc agcggttttc
     6661 attgcaaaag gcgtaagaat caagaagctg gcaaaagaca cggcaccacc gaaaatacct
     6721 tcaacgtcat aagattttga taaataatag ccgataccaa aagtaacaaa aaccgacatg
     6781 atgctcattg ttgcattttg cccattaccg aataaattcg ataaagttcc ctttgttgcg
     6841 tcactgaaaa aaggcaagtt attgataacg acaacgatcg aaccgaacat cgttaaaggg
     6901 aaggaaagca tgaaagcatc ccgcagcact gtaaggtaac gattttgccc taataaatta
     6961 gctaagggca tgattttctc agaaagcttg tccataaaac cgttcatttt tattcacctc
     7021 aaatttattt gcaatcgatt acatacaaag tataacgcca aaaattttaa atgtctagtc
     7081 attttcataa aaagtattga tttatttttt agggttcgtt atgattacgg tatcaagaaa
     7141 aaagagagga agtaaaaaaa tgaaaaccta cacattccca gaaaattttt ggtggggttc
     7201 tgctgctagc ggaccgcaaa cagaaggaag agtaagtgat gatggtaaag gagaaaatat
     7261 ttgggatcat tggtatgaat tagagcctga aaaattcttc gatcaagtag gacctgaaaa
     7321 aacttcgcaa gtttacacaa aatacaaaga agatgttcag ttaatgaaag aaactggcca
     7381 taacacattt cgtacatcta tccaatggag tcatttgatt ccagaaggga caggtgaaat
     7441 caatcaaaaa gctgtttctt tttatcgaga ttattttact gaattgctgg ataatggtat
     7501 tgaacctttt gtgaaccttt accatttcga tatgccacta ccactgcaag aaaaaggcgg
     7561 atggttatca agagaaacgg tcgacgctta tgtttcttat gcagcagcgt gctttgaact
     7621 gtttggagat ctggtcaaaa aatggtttac tcataatgaa ccaatcgtac ctgtagaagg
     7681 aggctacttg tatcaattcc actatccaaa tcatatcaat atgaaagaag cggtccaagt
     7741 aggctatcat gagaatctag caagcgcaaa ggtaatcaaa gcctaccatg aaggcggaca
     7801 tgatggtgag atcggaatca tcttgaatct gacaccaagc tatccgagag atgaaacgaa
     7861 cccagaagat gtaaaagcgg cgaagatcgc tgacgcattc ttcaaccgat cattccttga
     7921 ccctgcagtg aaaggagagt ttccacaaga gttgatcgaa attgtcaaag aattggctat
     7981 aatgccagag atccatgaag gagacctaga actcatccgt gaaaacacga ttgacctgct
     8041 aggaatcaat tactatcagc ctcgtcgaat caaagcaaaa gaaactccaa tcaatcacga
     8101 acatagcccc atgccagatg attattttga taattacgac atgcccaata aaaaaatgaa
     8161 tccatatcgt ggatgggaaa tttacgaaaa aggaatctat gatattttaa cgaatacaag
     8221 agaaaactat ggtaatatca aatgctatat ttctgaaaat gggatggggg tcgaaggaga
     8281 agaacgattt gttaacagcg aaggaattat tgaagatgat taccggatcg agtttgttac
     8341 ggatcatttg aaatacgtcc atcaagcaat ccaagaagga accaattgtg tgggttacca
     8401 catgtggaca tgtatggaca actggtcttg gacaaatgct tataagaatc gctatggctt
     8461 tatcgctgtt gatttggcac aagacggtaa acggacgatc aaaaaatcag gtcgctggtt
     8521 taaaacggtc tctgaaaaaa acggatttga tgcctgatat tttttgataa aacgttcaca
     8581 aaatgtttgt tctaacactg taatcgtgct acactaacag tgaaggaggc gtttacaatg
     8641 gtagatactt atcgtaatat tttagtagca attgatggtt ctgaaaaggc agaaaaagca
     8701 tttcttgagg caatcacgat tgccaaacga aatcaggcaa cgttgcacat cctgtatgtc
     8761 aatgaagtaa caggcaatta ctttggtgat tttgcatttg ttacaacaag ccttcaggaa
     8821 gaattggatg aggttgcgga aaaccagatg aaagaacatc gaaacttggc aattgaaaaa
     8881 ggcctgacag atatcgagac atatgttctt tacggttatc caaagacact gattgctaat
     8941 ttccatgaat cgaaagaacc aatcgatctg attgtcatgg gggcgacagg tctgaatgcg
     9001 gtagaacgtg cactagtagg ttccactact tcctatgtag tcaaccacgc atcatgtaac
     9061 gtcatggttg taaaataaat aaattaaggg aacagaaagc aaaaaagcat aacgcttctt
     9121 ttgctttctg ttccttttat ttttttgtaa aaaattactt tacctgatga agtaatcatg
     9181 ctattctaat tacaacgaca gatggagtaa tatggaggaa agaaaatgat ttcaagtgat
     9241 ggaatacgcg gatacaatga tgcgattatt ttatcgatcc ttaacaaaaa cgattcttat
     9301 ggatatgaaa tttctaaaac gattcgtgag aggacaaatg attcctatgt gatcaaagag
     9361 acaacacttt actccgtttt tgcccgttta gaaaagaatc aactgattgt ttcgtatcct
     9421 ggagaagtga ctcatggaaa aaagcggaca tattataaaa tcactgaaat agggcgtaat
     9481 cttttagaag aaaaaaagca agagtggctg acgacgaaag acgtagtcga tcaatttttg
     9541 gagggaaatt aaatggatac aatccgtaat tatttagaca gtctttttat cggagtacct
     9601 caaagtaccg agatagataa gctgaaaact gatttgttgg caaatatgga agaccattat
     9661 catgaattga tgggagaagg aaaaaatgaa caggaggcca tcggtacagt aatttcgaca
     9721 tttggttcaa tcgatgaatt attagaagaa ctagatgtcg aaaaaaaaca tcaagctgac
     9781 gagacggaaa caaacacagc ttctatttat ttaagcgaag cagaaaacta ctggaaagaa
     9841 tacagagcgg caagtctcca agtggcatca ggtgttctat tcattagctt atcttttgca
     9901 agttttcttt tcttttgttc agcaggatat gtgttcatgg gtatttcttg tctgattttc
     9961 ggtatagcat tagctgtcgg tttttttatt gcttctggta tgaaaatcac acgtttaaat
    10021 cactttttgc accatcggaa aatacctgaa aaagtattag cagaagcaaa agaaaaagaa
    10081 gaagagtatc agcgaagttt tggcttctca ctgattgcag gaattggatt atgtattttt
    10141 tcacttttcc cacttttggc ttctttaatg tggtatatgg atggtagtat cggtgcctct
    10201 atattttttg ttacggtagg aacgggtgtc ttcttgatca tttatggcag tctagtccgt
    10261 cattcttatc gtcaattcac tcaaagcgct tattactggt gaatgtattc agatttgtaa
    10321 aaatattcta tttatccggc gtagaaaaaa agac
//
LOCUS       DAIGOK010000098        10270 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.099, whole genome shotgun sequence.
ACCESSION   DAIGOK010000098 DAIGOK010000000
VERSION     DAIGOK010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.099"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            184..333
                     /locus_tag="NOA01_002053"
     CDS             184..333
                     /locus_tag="NOA01_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HCI0633236.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            344..1858
                     /gene="dltA"
                     /locus_tag="NOA01_002054"
     CDS             344..1858
                     /gene="dltA"
                     /locus_tag="NOA01_002054"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HCI0633237.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            1855..3060
                     /gene="dltB"
                     /locus_tag="NOA01_002055"
     CDS             1855..3060
                     /gene="dltB"
                     /locus_tag="NOA01_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HCI0633238.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            3105..3338
                     /gene="dltC"
                     /locus_tag="NOA01_002056"
     CDS             3105..3338
                     /gene="dltC"
                     /locus_tag="NOA01_002056"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HCI0633239.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            3341..4609
                     /gene="dltD"
                     /locus_tag="NOA01_002057"
     CDS             3341..4609
                     /gene="dltD"
                     /locus_tag="NOA01_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HCI0633240.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            4750..5832
                     /locus_tag="NOA01_002058"
     CDS             4750..5832
                     /locus_tag="NOA01_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCI0633241.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            5832..6635
                     /locus_tag="NOA01_002059"
     CDS             5832..6635
                     /locus_tag="NOA01_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HCI0633242.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            6705..7445
                     /locus_tag="NOA01_002060"
     CDS             6705..7445
                     /locus_tag="NOA01_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCI0633243.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            7456..8460
                     /locus_tag="NOA01_002061"
     CDS             7456..8460
                     /locus_tag="NOA01_002061"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCI0633244.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(8505..10202)
                     /locus_tag="NOA01_002062"
     CDS             complement(8505..10202)
                     /locus_tag="NOA01_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633245.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT         3298 a         1697 c         2082 g         3193 t
ORIGIN      
        1 ttttttatat tttttaaaag tacgagaaat tataaagaaa aaagcagtga tttgatgtgc
       61 tatttttata aaagctatgt gacaaaactg ttagtaaaaa aacggtttct gtaagctaga
      121 cacatgtttc tttttgtcaa aattagttac actttgtgtg aggttatatg aggagggaat
      181 ccaatgaaca aatggcgttc taatccaaaa ttgaattatt ggggaaaatt catcattcga
      241 acaatttttt atagtgcagt actgctgttc ctgatttatc tgtatcacta taaaaatatt
      301 caaggaggaa cttttatcta taacgaattt taggaggaag agtatggaaa tcaaaacgat
      361 tattgaagca atcgatgatt ggggaataaa tgaacctgat cgagttgctt atcaggcgga
      421 tgagacgcat acttttggtg aattgaaaca agcatcagac gcacttgctt attatttaga
      481 aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc aatttggagt ttgaaatgat
      541 tgtctcattt ctaggggttg tcaaatctgg ccatgcctat ttgccgattg aagagcatac
      601 acccaaggag cgtatcctat cgatctttcg ggtggctaaa ccatctatgg tcatcagtat
      661 cggggactgg atagaagata ttccttcggt tcctgtcata acaaaaaaag aatttcaaga
      721 aatcaagaag ataccagtag gattttatcc cagaaatagc gtaaaaggta cagaaaattt
      781 ttatattatt tttacttctg gtactactgg agaaccaaaa ggcgtacaaa tcagtcatga
      841 taatctagtc agctttgttc aatggacgat ggaagacttt gggatcaagc ctggtatgca
      901 tttcttagcc caagcgccat tttcttttga tttatcggta ttttctattt atcctgcatt
      961 agtttctggc ggaatgctga agccgttaaa taaagcagtt gttcaagact ttcgtcaatt
     1021 attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt tctactccat catttatgga
     1081 tatttgcttg atggaaccta cattcaatgg agaaaatgtg ccggaattgg acatgttttt
     1141 attttgcgga gaagaattga cgaaaaaaac tgcagaaagt ctacttgatc gattcccaaa
     1201 tgcacggata tataatactt acggtccaac agaagcaact gtagctattt caagtattca
     1261 gattgaccag catgtactag atagttatga ccgactaccg attggctacg taaaaaaaga
     1321 cacgaaagtt tctattgtcc aagaaggtaa acctgtttca aaaggagaaa cgggtgaaat
     1381 cattattgca ggaccaagcg tatcaaaagg ttacctgaac aatcccgaaa agacagctgc
     1441 tgcgttcttt tgtcaaaatg acttagcaag ttaccataca ggggatgcag gtagactaga
     1501 tgaatatggg cttttatttt atgaaggacg tatggatcag caggtcaaat tacatggata
     1561 ccggattgaa ctcggggata tcgaacatta tctgctccaa gacaaccgaa tcaaacaagc
     1621 tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag ctggttgctt ttgtcgtatt
     1681 agaaaacaaa acgaaacagc cggattttca gttgactaaa tctatcaaag aacaattact
     1741 agaagttgta atggattata tgattcctca gaagttccat tatgtcgatc tgctgcctca
     1801 gacagttaat ggaaaaatcg atcgaaaaaa attaacagct gaggtgaatc ctgaatgatg
     1861 ttacctcata tgatccctta tgcagagccg ttttattttt ttcttttagc cattgcgctt
     1921 gtaccgatcg ttctatcttt attgatatgg gaaaaaagat taccagttta tcaatcagca
     1981 gtgacacttt tctttttatt tgtcagcttt ggaggtaatt attggcaaca gggagcggcg
     2041 ctgattgggt atgttttgtg gcagacgata cttgtatggg gctattttgt ttatcggcaa
     2101 aggaaaaatc agacaggatg gttttttgcc gccgttattt tagcgataat tccactgttt
     2161 ttaacgaaag tgactccttt ttttgaacat ggaaaagcat cgttacttgg ttttttagga
     2221 atctcttatt taacctttaa atctgtacaa atgatcatgg aaataagaga tggcatgatc
     2281 aaaacatata agccatttcg ttatatccag tttctgctgt tctttccaac aatctcttct
     2341 ggaccaatcg atcgctatag gagatttgaa aaagatcagc tccatccgcc tgttcgtgat
     2401 aaatatgttg atcttttggg aaaaggcgtc caaaatattt ttgtcggttt tttatataaa
     2461 ttcattatcg gttattattt tggtcaagta ctgcttcctg tagtgggacg cattgcctta
     2521 cagcatggtg gtatttcatg ggcattagta ggttatatgt acgtgtacag tatgtatttg
     2581 ttttttgatt ttgctggcta tagcttattt gcagtcggta caagctacat gatgggatat
     2641 cagacaccaa tcaatttcaa taaacctttt ttatcatgga atatcaaaga attctggaat
     2701 cgttggcata tgacgctgtc cttttggttc cgtgattatg tatatatgcg attgatgttc
     2761 ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa tgtcaaatat cggttatttt
     2821 gctttgtttc ttctaatggg tatatggcat ggattgacgt ggtactacat cgtatatgga
     2881 ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga tccgatttaa gaagaagcat
     2941 aaagagcagt tgccttctaa caaatttacg catggcttag ccgtattttt gacatttcaa
     3001 gcagtatgtt ttagcttcct gattttttca ggatttttag ataaactttt ttttagctaa
     3061 cgaatagtga catattaatt tatataacaa aggagaaaag gaaaatggaa gaacaaattt
     3121 tatcaatctt agaagaaata acaggaacag atgaagtaag aaaaaatcgt gatattgatc
     3181 tatttgatga agggttgctt gattctttag catctgttca attattagtc gagctagacg
     3241 gagagctgga tatccaagtc cctgtatctg aattcgagcg agaggattgg agcacaccga
     3301 ataaaatcat tcaacaagca actgcattga agggttgagt atgaaaaaga aactttttgc
     3361 tattttcggc ccggttgttt tagctgttgg cttactgttt gtgtttttta cctcctccta
     3421 tcaagtagat atctctaatc cagaaacgat tagacgtgcc tcttcttcca tgtcagtgaa
     3481 cgtgttgaaa ggagatgcga taaaaaatta tgcattatct gaaaaacaat atattccttt
     3541 ttttggctca agtgaattaa gcagaatcag tccatttcat ccgtctgttc tggcagaaaa
     3601 ataccagcga aactatcgtc catttttgct gggagcaccc gggacacaat cgttaagcca
     3661 atatatgatg atgcgttctg caggagatgc tatgaaaaac aagaaagtcg tctttattat
     3721 ttctcctcag tggttcgtta aaaatggtgt aaaaactgat tactttaata cgtattattc
     3781 tgaacttcaa acatacgact ggttgttttc aatgaagaaa gtaacgcctg ctgatcgcta
     3841 tttagctaga agattattaa ctttctcaaa agtaaaagag aatgatacgt tgacggcgat
     3901 tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa tctttgaatc agctgcgcag
     3961 ccaatggaac atgctgaaga gagaggatga agtattcagc aatattgggt tgaaagatcg
     4021 acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa caataccaag aaacagaatt
     4081 atccatttta gcaaatcaaa ttggagaacg agaaacgacg aataatccat tcgggttgaa
     4141 aaacgatttt tatacgcatc gtatccgagc ccacgagcca gagctcaaac agtcacaaaa
     4201 aaattgggat tatcgttttt cgccagaatt ttctgatttt cagctggtct tggatcaatt
     4261 agcaaaaaac cataacgaag tattgttcat catcccaccg gttaacgaga aatggagcga
     4321 ttataccgga ctatctcaag aaatgttaca aggatttgcc aaaaagatca aatttcaatt
     4381 aaatagccaa ggattcaatc gaatagctga ttttgtcaat caagcgggta ccaactattt
     4441 tatggaagac acgatccatc taggctggaa aggctggttg gctgcagatc aacagatcag
     4501 accgttttta gaagaaaatc atattactgc tagtaagtat catcttgacg atgctttttt
     4561 tagcaaatct tggcaacatc aaataccaga taaactacaa ttaaaataaa gaaaacaggc
     4621 gagtgttgct cgcttgtttt ctttattttt gaatattctg aataaaagat aaaaatacca
     4681 agtttgcctg aacaaaaatc ataagcaatg gtataatcac aatatgaata tagaaatggg
     4741 ggaaaacatt tggatacaaa agaagaacag ctacttattg atttaacttc tgcaaaagga
     4801 gtacctggaa atgaagaaga ggtaagagaa gtattcagag aatatgccaa accattcgct
     4861 gatgatattt tttatgacgg tttaggcagt gtgattgcca aacatggtgc tggcggaggc
     4921 cccaaggtct ttatttctgg acatatggac gaagttggtt ttatggtaac aaaaataacc
     4981 gaaaaaggat tcttggaatt ccaaactctt ggaggttggt gggggcaagt catgcttgct
     5041 cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc acggtgtgat tggttccaaa
     5101 ccaccacatg ttttaacacc tcaagtaaga aataaacctt atgaaataaa ggatatgttc
     5161 attgatattg gcgcatcaag ccaagaagaa gccaaagagt ggggcatacg tcctggagat
     5221 atggtcactc catatattga atacaaacga atgaatggca gtaaatattt actggcaaaa
     5281 gcatgggata atcgtatcgg gacagctgtt tctctgcgag tattagagaa tctatctaaa
     5341 gaagcacacc caaatgtttt attcgcagga agcgatgtgc aagaagaagt aggcttgcga
     5401 ggtgctcgaa caagtacaca ccttgtaaat cccgatattg cttttgcttt agatactggt
     5461 acagcaggag acactccagg aatgacacca aaagaagcag attcgatttt aggaaaaggc
     5521 ccacagattt tgatttttga tgcatctatg atcccgcata aaaaattatt gaattttgtt
     5581 attgatgtag cagaggaaat ggatattcca ttccaatata cagtgatttc tggcggagga
     5641 actgatgcag gacaaatgca tttgacaaga gatggtgttc cgtctgtagc cattaccgtt
     5701 cctgttcgtt atctgcattc ccatacatca gtgattcatg aagatgatta tttgaataca
     5761 gtgaaactag tcacagaatt agtgaaacgg cttgatagtg aaacagttga aaaaattcgg
     5821 agttatgaat aatgaataat agaataccta tttctgaata tcggaaaaaa gcacgagttt
     5881 ctttggaaaa tcaatgggga atcaatactt ggcttgtttt cttatctata atatttacga
     5941 gcgtcatcca gaactttttt gacggtttca gtccttttcc ggaagaaagc atgcagcagc
     6001 acttattgag ttttctagtc gataatttac ttttattcgc attcaactat gcattgtttt
     6061 acattgcgct agtagttgta cggggtggca gaccaaaaag taacctttta ttttcagttt
     6121 tccaaagaaa gtattttggt tcgatacttg gcatcaattt gatcaattca gtcatcaact
     6181 ggctactcaa cagtttaatc tttctgccca tgtttttaat cagcggagtg agtacttata
     6241 tgcagctgct tttcagttca aatgcggtac ctacgggtgt catgccagaa gattttcttg
     6301 atatcagctt tgttttatcg atgataggga tgatttttgt ttcagcactc atcatgagta
     6361 ttgttggcgg tttattccaa tttgctgcat ggacaaagtt cgactatcct gatttgtcgg
     6421 ttattcaatg tttgaaatat ggctggtttt tattgaaaga tagaattggt acgtatattt
     6481 tgttgcagct gtcttttatc ggctggtatt tgctaggagc aatcgctttg ttcatcggtc
     6541 ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga attttatgaa caagcacgga
     6601 ttgaaaaagg aggtccagac gcctatttta cttagcatgt aagcagttta ttcatcaaaa
     6661 aaataacttt tgtggtagga taaatctttg agggggaatg tacgatgaaa ttaactgttt
     6721 taggatgctt aggagcgtat ccttataaaa atcaagggac aacaggttat ttgcttcaat
     6781 ctgacgggtt caatttgttg atcgatgcag gtagtgctac gctgatcaaa ttacaagaac
     6841 accttgatcc acttgatcta gatgctgtga tccttagcca ttatcatcat gatcatattg
     6901 ctgaccttgg tgtattacag tattactggc aacttcatcc taagagaaat ccggagacat
     6961 tgctttcaat ttatggacac acaaaagatt ccattcactt tgaagaattg acgttggaaa
     7021 atgtctcaaa aggacatcct tattttgaag ctgaagaatt aaaaattggt ccttttctga
     7081 ttacttttat ggaaacaatc catccagtag tatgttacgc catgcgtatc gtagaagaaa
     7141 caacggggaa agtattcgta tttacaggtg attcaggtta tttggaaagt ttcgctgatt
     7201 ttgcgaaaga tgctgatttg tttctagcag atacctatct ttttgaagga aatgaacatc
     7261 atcatgccca tttcacttca aaagaagctg gagaacttgc gaaatcagct aacgtaaaga
     7321 aacttgtact tacacaccta cctcagtttg gtgatttaga acaattgaaa aatgaagcgc
     7381 aaaaggcagc aggcaaaggg attgatatag aacttgctgc agttgacaaa gtgtttaaca
     7441 tttaggagga agaacatgat ttttgtacca aatgaaaacc acgacccaag aatcaattta
     7501 gcaattgaac aatatttgct gcaagaaatg cctttagatg aacctatttt gctcttttat
     7561 atcaatgaac cttcaatcat catcggcaga aaccaaaata cgcttgaaga aatcaatcga
     7621 gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct taagtggagg cggagctgtc
     7681 taccatgatt ttggaaacct gaactttagt tttatcatgc ctgatgatgg ggattcattc
     7741 cgtgactttg cgaaagtaac acagccaatc atccaagcac ttcatgaatt gggtgttgct
     7801 ggggcggaat tgaaaggccg aaatgatcta gtcattgatg gcatgaaatt ttctggcaac
     7861 gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa caattatgtt cgactctgat
     7921 atcaatgaag tagtcaatgc attaaaagta aaaaaagaca aaatcgaatc aaaaggaatc
     7981 aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc ttccagaaga aaagcaaaaa
     8041 atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa ttttcggcgt agagtcagta
     8101 gaagaagtaa aaacgtacga attgactgat gaagattgga aaaaaatcaa tcgaatctct
     8161 gaggaatatt atcgaaattg ggattggaat tacgggaagt ctccagattt caatttctct
     8221 cgccaaaaac gtttttctat cggttcgatt gaagtccatt tgaatgtctc agaaggaatg
     8281 atcaaagaag caaaaatctt tggcgatttc ttcggactag gagatatctc tgacgtggaa
     8341 aaaaaattgg ttggacagaa atatgataaa gcatctctat caaaaatagt cagccaaatt
     8401 gatgtgaaga aatattttgg tgcgatagaa ccagaagaat tgcttacctt actttattga
     8461 aaataaaaaa tagcggaatc cacatatatg gattccgcta ttttttatac aggattcggg
     8521 taatttcgag agagttgaac agtgatccga tcaatgaaag tatctttttc ctgcgttagt
     8581 gttcctgtgt aacgatccgc tacgtctttc atcatcatta agtcaaaacg ttcctgatca
     8641 tctaattctt ctgtgatgcc tcgactaaat aggtttgtat cgtaatgtgc actatgttcg
     8701 caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca aataaatttt tcgatacagt
     8761 ggattttcga tggcatgagt ttctctaaag gaatgctcta aagcgtggat caaaagttga
     8821 gccagatcgt catcgttgat aggaagttct tcgggaagat ctgcttggaa ggttatctga
     8881 atattccgtt tggcagctaa cttctggtaa cgcgctaaga taagatttgt cacctggtgg
     8941 ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt ctaatatctt ttccacatag
     9001 tttttagctt ttgtttggtc cgtatgcatt aaatcttgca gattctccat ctcttggaca
     9061 aattccaggc gaagtgtatt tacttcttgg atatgctgtc gaagctgctc ataatgttgc
     9121 tccaacaaat catttttgat ttttaagaaa tctacttgtc tccgatcttt gtcggtattt
     9181 aagatgtgat cgattagatt gtagctgata ataacgatta gaatgataat atataaaatc
     9241 gtagaccaat tgatcgttgt ttgatagata cctaatgccg tttggatgta aatcaagcaa
     9301 tggataatgg ctaccaagac gatccaaggt gtacaagcta cataaaaacg gttattctta
     9361 tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc ttataacact agcaatatta
     9421 gcaatcggca ttaattcagg aagttctgca ggagtaaaag ttgcataaag cagaacgccg
     9481 ataaaaatca aaagttggaa cgtacaaaaa attccgtacc attttttata gtaaaacatt
     9541 cggctccaat agtagcagat gatgaaagct gacgttaaga taataaataa atgagataaa
     9601 aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa tatcttctga tacagcttca
     9661 aatcctaatg ccaaagcaaa gcaacttaag agaataagtg agtattcaag acgtttttgt
     9721 ttatacatca agtagatcgt atagaaaaag gtccctatag cgataaaaac agcgattatc
     9781 atcgtcaata cttgcggcac tgattgtgaa taaataaaac taatcaatgg tccagttgtt
     9841 cctagataaa ctttcggtgg cagtccagca tagttctgat aaggagatga gactgtaagg
     9901 gtcaattttt ttccgatata atttgctggt aaatctatca cagccaatga gagtccggga
     9961 ttgctttctt cctgataatc tttccgttgg tataaaatct tttcgcctag atatacagta
    10021 atccattgat ggtttccttt gattagtaaa tttgcttcgt ccaacggttt ttcaagtgtc
    10081 gtttccataa tgaatgtttc gtctttatcc acggtaggaa tcgtacgagt gtacttgctg
    10141 tcaaacttaa aatcaggatc atcttttgta tagaaaaccc acgaatcttt tatttctttc
    10201 atttgattgc tgtccgcagg agaaaaaata aaataaaaga aataagcaat tcctgttaaa
    10261 aataacaatg
//
LOCUS       DAIGOK010000099        10122 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.100, whole genome shotgun sequence.
ACCESSION   DAIGOK010000099 DAIGOK010000000
VERSION     DAIGOK010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10122
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.100"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(560..1600)
                     /locus_tag="NOA01_002063"
     CDS             complement(560..1600)
                     /locus_tag="NOA01_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCI0633246.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1949..2275)
                     /locus_tag="NOA01_002064"
     CDS             complement(1949..2275)
                     /locus_tag="NOA01_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633247.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2262..3065)
                     /locus_tag="NOA01_002065"
     CDS             complement(2262..3065)
                     /locus_tag="NOA01_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCI0633248.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3418..3708)
                     /locus_tag="NOA01_002066"
     CDS             complement(3418..3708)
                     /locus_tag="NOA01_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HCI0633249.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(3844..4023)
                     /locus_tag="NOA01_002067"
     CDS             complement(3844..4023)
                     /locus_tag="NOA01_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633250.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4020..4370)
                     /locus_tag="NOA01_002068"
     CDS             complement(4020..4370)
                     /locus_tag="NOA01_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633251.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4363..5688)
                     /locus_tag="NOA01_002069"
     CDS             complement(4363..5688)
                     /locus_tag="NOA01_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCI0633252.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLNIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6138..6752
                     /locus_tag="NOA01_002070"
     CDS             6138..6752
                     /locus_tag="NOA01_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCI0633253.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(6917..7741)
                     /locus_tag="NOA01_002071"
     CDS             complement(6917..7741)
                     /locus_tag="NOA01_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633254.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(8378..8635)
                     /locus_tag="NOA01_002072"
     CDS             complement(8378..8635)
                     /locus_tag="NOA01_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCI0633255.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(8628..8897)
                     /locus_tag="NOA01_002073"
     CDS             complement(8628..8897)
                     /locus_tag="NOA01_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCI0633256.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(9028..9207)
                     /locus_tag="NOA01_002074"
     CDS             complement(9028..9207)
                     /locus_tag="NOA01_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633257.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(9588..>10122)
                     /locus_tag="NOA01_002075"
     CDS             complement(9588..>10122)
                     /locus_tag="NOA01_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633258.1"
                     /translation="QTKVTVSHNRLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
BASE COUNT         3098 a         1842 c         1454 g         3728 t
ORIGIN      
        1 ccgttgcggg taataccgta acactgaagg gcacgcatta aggcgtcatc ttaatgcttt
       61 cctgtaggac actgacggag attaccagtc tcccgagcct tacaggggcc tttctttttt
      121 ttgtattttc aattatcaca atttatcgtc caaaagtgca gaacgaaaat caattcatca
      181 caagcatatc agacaattaa atttaaaaca actaaaaata tacaaaaaag tgtaattgaa
      241 tattagcagc actgcctgaa aagaagtaat atcaggcttt tcagcttggg gaaataagtg
      301 aattacctct cttcatgaat tattataact caaaaaagca catatgcaaa cactagttcc
      361 ctattttttt gaatgaaaaa taactaaaat atctaacgat tatgacttta aaacctacgt
      421 ttgtgattgt tttcataaac ttttaatcta aaaactccgg aataatagct atttatttgt
      481 aacatttgta ataaaattaa aatcataaaa aagttctaat ttaaaattga tcatttttat
      541 agtgatcgct ttttatcaac tactctagcc aattttccgt actaactgtt ggtaattcgt
      601 taaatgtttc tctttgttcg tttgcaagct ttgccgcaat agctttttcg acaaaccaat
      661 tttttacata aacgaaaatt agattctgca aattgtttac tttttcagtt ttttgttttt
      721 gataagcttt cagcaaagtc atatataatc ctttgtcagc atcaattcca taagattcca
      781 gttcttcata aacaacaact gtttgctcta attcttcagc ttttttcttt gcattatgaa
      841 tagtttggac ggttttttga gcgtcttcgt aagtactaga aaatgttttt atcaagttta
      901 atacattttc tggtacaaat gtatttatac tgtcctcttt catgatgtcg acgaagttat
      961 ctagtaaaac ttgattttgt aactgatcag tttcagtgtc tattatgtgt ctattagtgt
     1021 ctatagaatt agaatagaat agattgtctg cgattttcgc aattacggtt gagtccttga
     1081 tatgactgga ttttgccgta tttgcgattt tcgcaattac ggtggtatca ttgatttctc
     1141 gcggttttgc cgtatttgcg attttcgcaa ttacggtggt atcagtattg tcttcctctt
     1201 ccatgatttt aaagacgtct tgattcgtta cttctggctt gcctaagtac aataaattag
     1261 gcatggattc aatcttaccg tcacgtttat ttactcttcc tctcttctgt ttcagaagac
     1321 ctgctttttc taattccttt ttgattttag atactttacc ttcgcggcat tgtaacaatt
     1381 tcattaattc ttcaactgtg aatatgaaaa atatattatc atcagaatct acccagttat
     1441 ttttgactga ataatcaaat cgatctttaa gtagcatata agcaattttt gcatcatttg
     1501 atagatccat gtatttttcg ttagtaaaaa ataccttagg catttgataa tatttttcac
     1561 gataaatttc ttttgatttg taataattaa aatcattcat agccaatcct ctttctgtag
     1621 gatagcgttt tgttcaaaac tatgttacaa tgaattaaac aaatatatct atccactata
     1681 tatttttcta ctgctgataa gtcgccaaac tttgttaaag cagtagtctt tttttatctt
     1741 tttttagttt accttaaaaa ataaattaaa gcaaaccgct ctatttcaag gtttaagctc
     1801 atagtaacta tatcacgata ttacaataat attatatcac gatatcgtaa tattacaata
     1861 tgaagataag cgacttatca gctttgatac tactgatttt tataaaaaaa atatcacgat
     1921 attatgatat cgtgatattg aatgtaaatt aaattttatt ttgtttcttt gctagtttat
     1981 atccttcaat caagtattct tcaggtatgg actttaatag cgcatcttct ataacgtctg
     2041 gtttactttt gtctgattca gcaatagtcg tccaatattt gagtgctgac attagaattg
     2101 gatttatacg aatgctatct gctgttctta cactacttcc ttgatctttt atgactttac
     2161 ttatattttt cttggttaat tcgatttctt ctacttcttt tgatttaaaa ttctttttat
     2221 ttttatttcc tagtaatcta cttactgcag cttgactgcg attatcttct gccatcaaaa
     2281 tcaccaactt cttttaaata ttcttctgta aatgctttat agtcatcttc agctgttgag
     2341 ggtttattaa aattgattag atctgatcca gcagcttgcg cttcgtcaac ggttattgtc
     2401 ctacgaatat aagttttgaa aatttttgca ccaatgactc tagaaatttc ggttgctgca
     2461 gacgtcattt ctttagaaat gttataacgc ttattatact tagtaaatag aactcctcgt
     2521 acttcaagct tgtcgttcgt atattctttt accgtttcaa tagtttcact taactttatt
     2581 agtcctttta atgagaaata cgcagcttca actggaatga taatttcatc agaaacagta
     2641 aatgcgttga ctgtaagcat tcctaaactt ggtggacaat caattataat ataatcatag
     2701 ttgtccttga ttggttgaag tactctttta agcctgtgtt cacttcctac ttgagtaaat
     2761 tccctctctg cagaagaaag ggctatgtcg gctggcaaaa catcaattcc tttatagtcc
     2821 agaatagcat ctgtaatgtc agcctcccct tttaatactt cataaattgt aggttggttt
     2881 gttccatcta tatcgaaacc tacattatct gtagcatttc cctgaggatc aatgtccact
     2941 aaaagtacat ttttaccgta aacaaatttc aaacatgctg ctaaattaac gctagtcgta
     3001 gttttagaaa ctccaccttt ttgattggct actgacaata ttcttgccat taaaatatcc
     3061 cccattcatc ttatattata atataattat atcataatat tgtgatattg caatatcgta
     3121 tatgcattat attgtaatat tgtgtgatta ttttgtttta aagatatata tcggttcgga
     3181 tataaagatt attcatatgt tattcgaaat taaaataatc actttttatt actttaaaat
     3241 tttttattaa tgataagaaa tatgttttgc gagcagcaac tagaataatt taaacattac
     3301 taaactgtct atattgttac taataattcc cttcttatag tgcattttct tagaaaaaaa
     3361 caatattacg atatcatgat attaagatat cgtaatatta tacaattgtg attgatatta
     3421 tatatgtttt tttatttttt tatcatgttt aatcttgaag gaatcatatt ctttttctaa
     3481 agacaggagc ttttcataaa catcagacat gtttaaacta gaggccaggc ttaaatcata
     3541 aacgaatgaa gcaggtaatg attttattct acgttgtcgg gttatccagc ttgcaaccgt
     3601 tccttgtggg taggcatata actcacaaaa ttgttctaca gtgattccta aattcttgat
     3661 aatgtaaaca ttgatcgggt gcgagtaaac aaatgtattg atagacatgg tgcgtaagcc
     3721 tactttcttt tattattgcg tttgcaataa tagtttaaca cacgcattat tctagtatct
     3781 actcctattt gtaaaaacaa aaaaggattc atagagataa ctctatagaa tccgtaaaat
     3841 accttatata tataacggga agatataaaa cctaaaatta tgataacctg ctgcagcttc
     3901 tacatagaaa gagaagcgct caacaccaac ctcatttatc aaacgttggc cataacccca
     3961 aatctcttct gatagctgat caaaggttat atactttagc tcatattgct ggataatgtt
     4021 cattctaatc gactccattt attatcctgg taaaattcga tatgacgtat ttcgtcgtag
     4081 tgaattttgt cttttccaac gtagatcccc agctcatcac tacctaagat cttgccaatg
     4141 atatcttctg gatagtatcc ttcagcgttt cgttcttctt tttgaataga tactgatcta
     4201 ttttttatga ctgcagcgtt caatacttcc gcaatctctt cagtcgtcat ttgttctttt
     4261 gctaaatttt gactggttcg ttgttcattg tcggcatcaa tttcttcagt atgatcagat
     4321 aaataaaacc ctgcccactt taaacgtcca cgatcattgt aatcaatcat ttttaattcc
     4381 cccatttccg cccgcatgac caccaacaag tgtgcttcta gtaatgcttc tagcgccttc
     4441 taacattgaa gaagcatgta caatcgacgt aaaaccatat ttttgccgaa tcttgtcaat
     4501 aattttatct aaattttctt cgtcaatttg cttttgtgga tcactaaata aatccagctg
     4561 aagcgaatcg gtgtacacaa gcttgctata ggttattcca acgtgcctta cttcttgtcc
     4621 ttcataatac ttatcaaaca agaaaagaca ataattctgc aattcctttg tattatctgt
     4681 gataggtatt ttcatctggt gtgaaaatcc tggtctcgtt tctaagatcg acgtaccaat
     4741 gtttaaatga acacatcctg ttttacaatt atgctgcctg atacgaatgg ctacttgttc
     4801 cgccatttct tttaccacca gctcgatttc atttcttcta gtataatctc gtggtaacac
     4861 ttgtgaatta ccataagatt tttcctttgt cggttctggc gggattgcta tatctgttcg
     4921 atcgattccg ttagcatgga agtacagctg taatccaatc acacctaatc tattcttaat
     4981 cgtataagga ttccagttag caaggtctct aatgctcaaa attcccattt ggttgagcct
     5041 ttttttcatt cgcgaaccaa ttccccaaaa gtccgtcatt tcgggaatat tccatacttt
     5101 ctcaggaacg ttttcgtacg tccattccgc aataaatcct tcattatgct ttgcttcatt
     5161 gtctaaggct aatttagcca aaaggggatt atcgccgacg cctaccgtag caatcaaacc
     5221 taactcttct ttaatacgtt cttggatcat ttgagcgagc ttcttccttc gctgacttcg
     5281 tgtttcttca gtcgtaaaaa gattcagtga ttttgtcact tttaaaatcg attcatcgat
     5341 cgagtaaatc agtagatctt catcagccac atatcttctg aaaatattat ttacctgcat
     5401 atttcgcttg atatataggt tcatacgtgg tggaacaacg tgtagtgttt taggaaatgg
     5461 ttgtggtaag tcacgtggtc tactcacatt tgtaatacca taccgctttt ttgcttcagg
     5521 agaagaagct aatatcaatc ctgaaccagt attatcagat cgactcataa caacaagttc
     5581 tgttgttaat ggatctaaat ttctttctat acattcgaca cttgcgtaaa agcttttcat
     5641 atcgattaaa aagtaatcat ttactggctc ttttgaataa tccatcataa ataacactcc
     5701 attcttccac attatacaaa catatgttcg atgttgtaaa gaagaaatag ctaaatacat
     5761 aaaaaaagaa gcataggtat gctatcaccc tgcttcttct tttgatttgt atgatcaatt
     5821 tgttctatca ctttactaat taatttgaaa atctaataaa aactctcact ctaaaagagt
     5881 gagagactgc aaattagagt acggactttt ttggagttcc gttctttaat attaacaaat
     5941 gaataaaatc cttacaacat ttgagaataa gttatcagta gacacttaca aaaaattctc
     6001 atactatttc ctccatcatt agtttttgtc tatataaggt ttaccctaaa tagacactgg
     6061 tataacattg aaaaatatgg cttcctcgtg tatgattagg gtgtatttta aatagttgca
     6121 gaaagtaggt aaaagttttg agagttgcat acatacgtgt ctcatcgatc gatcaaaatg
     6181 aacaacgcca gattgaagaa atgaaaaaat tcggtgcaga gagaattttc atcgagaaac
     6241 aatctggcgc aacgattact catcgacctg ttttccaaga agcattagat tacgtccgag
     6301 atcaagatat ttttattgtg gaagctattg atcgacttgg aagaaattat gacgagatca
     6361 tcgcttcagt caattacttg aagaagaaaa atgtgaagct gatcattact agtttaccga
     6421 tcatggctga agcaattggc aatcctctcc tggataaatt cattaaggat ctgatcatcc
     6481 aaattttggc catgatcgct gaacaagaac ggactgaatc gaaacgtcgc caggcacaag
     6541 gaattaagat cgcaaaagca aacggcgtct acaaagggcg gcccaaatta tacagtgcag
     6601 acgcaaaaga tccccaacgt cgccttgttt acaaaagcat cgttgaagat cttaaaaatg
     6661 gtgttgcgat tgctaagatt gctaaagact acaatgttac gagacaaacc gtgtatagaa
     6721 taaaaaaaga cagcatggtc aatgacaaat agttgatcat gctgctttta ttatcttctc
     6781 atctttaatt tcctacatat aattactatc tataaaatta agtcttactc agaacagata
     6841 aagcataaaa ttccaacaaa ccatttttta cttaactaaa taaagagtct gttaatataa
     6901 tcttcactta tttacgttaa tcccctactg tatttgttgg ttccttatct atattttcta
     6961 caaaatcctt attatcttgt gatttagctt cgtataattc tttgtatgtc gacacattgg
     7021 ttatactaat tcctatagct agttcttgag actctacttt tctctctagc tcctttgaca
     7081 aagtgatttg agatttattt ggttccgttg gagtcaattc tatagattct tcataaaaat
     7141 ctctatacat aaagctatca ttaattttat aaaaaaaatt aaatgttttt agcactgcca
     7201 tataacattc aaaacattct tctatagttg gtttacttcc ttgaggagaa tgaaccaatt
     7261 tattacgttt ttctatgaaa tttttaattt cctttcttat ttctttcgga aactcaatat
     7321 taaagtaccc tttaaacatg ctccctaata atttgtctac agggggagat tgtaaattct
     7381 caaatattag agttaaatca ggtttctctt gttgataaaa ttcctttacc cctatttcac
     7441 aggctgtaat tatcgaaata aacctatctc tagtatttga tatattttta cttcttttaa
     7501 ggagtataag agaaaagggg atactttcgt gtccatgcat gtaattaact aattgttgag
     7561 cttcagaagt tatatctcta attatatagt taacggaatt aggaagtgtc tttaactcta
     7621 atgaaatttc tatccaatta ctatcttcta atctgtatat gctcgaagta taatttgatt
     7681 cgtcagcaag catcgctaca tcccaaacac tgatcataaa atcttttaaa cgttcaatca
     7741 tttcagcaat attgctaaaa tcagctaatt tttctttaga ataaaaaaaa catttatatc
     7801 agaatcaata aattctattt ttttattact atatcgtcat cattatcttg accatttatg
     7861 tttattgttc cctgagtggg gagtacttct ttcaaattca agttaaattt atattccatt
     7921 acttctcctc caatatataa aaattactta actgtatcca aatttaatat gtaaataaat
     7981 cattaatttt tagctaaatt ctatgagaag tttatttttg ttgtttttgc taattaacta
     8041 aaactagtac aaaaaattta aaatagatta ttaaacctat tgcttattct attcagtcat
     8101 atatataagc gtcatccttt agtaactaac atcccacact acaatagtat tataacatat
     8161 ctattcaact tcaaattgtt gaaatttaag ccatttatat ttacttttcc tcaactttag
     8221 aaatgatttt actaacctta tatccgtaat acgcgtaaca aacacattca ttaataacaa
     8281 tcaacactaa gctatattag ctttttaaat gactagggta aataaaataa ggatcaaaaa
     8341 cttttaagtt tctgaccctt tccttacttc cgattggtta atagtgatct tttgcagaat
     8401 aaataaatat cgtttcattt tcaactctat atatcagtct atgttcatct gtaattcttc
     8461 tggaccattt tccagataaa tcatgcttta atggctcagg ttttcctaat ccagcaaagg
     8521 gggaacgatc gatatctttt attaacttgt taatcttttt tatattgctt ttgtttcctt
     8581 gctcatgcca ataaagataa tcatcccaag catcatcaga ccaagcctta atcatcagat
     8641 tcaacctcga ttaagtcatg tgttttaaat gcacctttgg agaattgttc atctcctcga
     8701 cgaatttttt ccatgacgta attattagaa agtgttctca acgtttcttg catagaatca
     8761 taatctcttt ttgataatac aacaactgta tcttctacat ctttacttgt tacaataagt
     8821 gtttcagcat cctcattaac ttgtttcata taactacgta aattttggcg gaaatttgaa
     8881 taagctactg cttccattcc tttcacccct tatttatttc tataaaacaa ttgtacatta
     8941 tattgtacaa ttaagcaaat gattaatatt ctaacttctt ataattaaaa gtgctatcac
     9001 ttttagtttt taggataaaa agtgacgtca ctttttgagt gctggtacct caaatcctgc
     9061 tttttcttgg gcaatcaata aatctaacgc aatacacata atcctggatt cacttttgcc
     9121 gtacttctgc tttagttgct gcaattcttt ttgtctcttt tcatccaacg taacgggtaa
     9181 ccgtttattt cctattgcaa ttggcattag tactctctcc tttcatcgaa ataaccctat
     9241 gaaaaatatg gctgcaatca tcttgaatag tagactcaca agggctaata caaagcgcca
     9301 aaagatcaaa caaaatacca gtattgccaa aaatactacg aagtttaaat gcacagctgc
     9361 agcatggcgc ataagtattt cgccaacaaa taggactaac aatagaggaa tgattttatt
     9421 catatggatc tccttctttc tcttaggtta tcgggtgttg cccgcttatt tcgcaccacc
     9481 taagcggcgg gggcggttgg agcgattggt gcgaaataag aaccattccc ttagttacta
     9541 ggttttttgt ccttatttca cacattctca cttttcggta taaatgatta ttgccttttt
     9601 ttcctttcaa atcgttgata tggcattcgt ttacggtcaa gttcattttg gatttcttct
     9661 gtatcgtcgt aagtataggt gtctaaaaaa tcagttaaag tcgtgtacca gtcgtcgcct
     9721 gggttatcta cttcttctac atattccctc aaaaatgctt cgggtacagc gagttcataa
     9781 tccattccca ttaacacgat agattccgtt ccacacgtca aaaatctagt aaatacaagg
     9841 taataatcgt ctatcgtcgc atctttgctg tttagctctt ccatttcttc ttccattaat
     9901 tccatgctct caaaaattga ataatattct gattctgagt aatagtgttt cctgcaaatc
     9961 gtgtagctga caaagttttc gaccagctca ctcctttcta ggtaaacatt catgatgtat
    10021 ttttctgttt ctagtcgtag aagctccgtt ccttttttta ccgtttcagg acgacgttta
    10081 tacacgtcta aacgattatg gctgactgtg accttcgttt ga
//
LOCUS       DAIGOK010000100        10036 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.101, whole genome shotgun sequence.
ACCESSION   DAIGOK010000100 DAIGOK010000000
VERSION     DAIGOK010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.101"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            67..1023
                     /locus_tag="NOA01_002076"
     CDS             67..1023
                     /locus_tag="NOA01_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCI0633259.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            1096..1212
                     /locus_tag="NOA01_002077"
     CDS             1096..1212
                     /locus_tag="NOA01_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HCI0633260.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(1267..2949)
                     /gene="recN"
                     /locus_tag="NOA01_002078"
     CDS             complement(1267..2949)
                     /gene="recN"
                     /locus_tag="NOA01_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCI0633261.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(2967..3416)
                     /locus_tag="NOA01_002079"
     CDS             complement(2967..3416)
                     /locus_tag="NOA01_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCI0633262.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            complement(3523..4341)
                     /locus_tag="NOA01_002080"
     CDS             complement(3523..4341)
                     /locus_tag="NOA01_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HCI0633263.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVTVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            complement(4350..5240)
                     /locus_tag="NOA01_002081"
     CDS             complement(4350..5240)
                     /locus_tag="NOA01_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCI0633264.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            complement(5242..5466)
                     /locus_tag="NOA01_002082"
     CDS             complement(5242..5466)
                     /locus_tag="NOA01_002082"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCI0633265.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            complement(5471..6808)
                     /locus_tag="NOA01_002083"
     CDS             complement(5471..6808)
                     /locus_tag="NOA01_002083"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCI0633266.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            complement(6809..7663)
                     /locus_tag="NOA01_002084"
     CDS             complement(6809..7663)
                     /locus_tag="NOA01_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HCI0633267.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            complement(7760..8209)
                     /gene="nusB"
                     /locus_tag="NOA01_002085"
     CDS             complement(7760..8209)
                     /gene="nusB"
                     /locus_tag="NOA01_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCI0633268.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            complement(8202..8627)
                     /locus_tag="NOA01_002086"
     CDS             complement(8202..8627)
                     /locus_tag="NOA01_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCI0633269.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            complement(8873..9937)
                     /locus_tag="NOA01_002087"
     CDS             complement(8873..9937)
                     /locus_tag="NOA01_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCI0633270.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
BASE COUNT         2809 a         2237 c         1657 g         3333 t
ORIGIN      
        1 acacattcca tgtattccaa ttccctatcc gttataataa aagaaacagg caacaggagg
       61 aaaatcatga tcaaatattt tacattagat aatgagaaac tgcgtaatac cggaaaggaa
      121 actgaccaaa cgatctggta ttgtgtagaa cgtcctaatc aagaagagat tcatcagctg
      181 acacatcaat ttgaaattcc gtcagattac atcacttcca tattggacga tgcagaaaat
      241 tcccgtacag aagaattcaa tcaatcaaag ttgacaaagc cagcgttatt attgttacaa
      301 tatccttttg ctaaaacaag tcctagcggt tattttcaag tggatacata cccgctctct
      361 attattttga cgcccaaaaa gaagcttatc acaattacga atcatgaacc actgttttta
      421 aagaaagtct ttagccagac ctttccaaaa agtgatattt cgcctaatct caatatcttc
      481 tttcaattca tttggcaggt cacactctct tacaacagtt atttagctac gcttcaatct
      541 caatgcgata ctttggaagc caaacttcaa gtatccactg aaaacaaaca actttatcaa
      601 atcatggata tccaaaaaag cctcgtttat ttcagagaag cgacttctgc taatttagat
      661 gcattgaaag aactagcgca aaataaactt ttccaagaaa atcatgcttt gaaaaatcat
      721 ttacgtgatg tattggtgga aacagagcag gcaatgacta cttctcgcat tcaattaaaa
      781 ctagtagaac aaatgaatca gactttttca gcaatcgtat cgaataatct gaataatatt
      841 atgaaaattt taacttcttt aacgattatt ttaactatac cgacaatcgt cggcagtatc
      901 tatggaatga acattaagtt acctattgct gaaagggatg atgcctttat atggctagtt
      961 ctattaacat tatttatttg tatcgtaact acctattatt taaaaaaagg gaagttttta
     1021 taaaaaagcc ctcttgtgga aatggaaaaa agattgtaca cttgaatcaa ctaattaatg
     1081 acgaggttga aaacaatgcg tgaaaaaaaa caaacaagta cttttgctaa agtaacaaaa
     1141 tttgtcattt ggacaatgct gattttaacg attggatctg ttgtattgac tgctgtaatg
     1201 agtgtcatgt aaaaataaaa tatagctatc agctgctgaa aaggacctga accactgcga
     1261 ggtggctcag gtcctttttg cgagtttcaa tagttctttt gcatgttcga gtgtgagttt
     1321 tgtaatctca cttccagcca gcatacgtgc aatttcttct tctctttctt ttgttttcaa
     1381 ctctgaaaca gaagtttgcg tacgtccatc aatcactgct ttcacaatat aatattgata
     1441 gtcggcgact gcagcaactt gaggcagatg agtgatgcac aaaacttgag agtatttcga
     1501 tatttgagaa attttatctg cgatggcctg tgcaactctt ccgctcacac ctgtatccac
     1561 ttcatcaaaa ataatacttg tcacaccttg ctcagaagaa aaaatagttt tcaaagcaag
     1621 caacatccga gacagttcgc caccagaagc gactttcacc aaaggcttca gtggctctcc
     1681 tggattggtc gtaatgtaaa attcgatttg gtcaaatcca tcatgattca attgtctatt
     1741 ttcttctttt aagaaacgta cctcaaactc agtgttttcc atatacagac ttttcaattc
     1801 atgcagaatg gatttttcca attcttttgc gattttttta cgcgcttcat gaagatctgc
     1861 ggcttgttgc tgaagtagaa gctctttttg ctctagttcc tctttcattt tgtccaattg
     1921 accttctgaa aaatcagaag aagctagctc ttcggtgatt tcgtcatagt aagccaatat
     1981 tgactcaatg gattcgccgt atttacgttt caactgtcgg attaattcta aacgttgcgt
     2041 aacttcttct aaacgactct catctaactc caataaatca atctgacgac tcatatcgcc
     2101 gattgcatct tgcagcaaat agtaagcact ttggatattt tcataaattg cttcatactc
     2161 cacatctaga tgggcgatcc cttgcagttc tgaaacggcc aagccaacac catctacact
     2221 gttttgttca ttattcgtca gtgctccgta acctgcagct aatccatcag caattttttg
     2281 ataattgcta agtttttctc tttcttcttt taatttctct tcttctccga cttgcaaatc
     2341 agcagcagct atttcttctt gctggaaatg gagcatatct attctctgaa cataggattt
     2401 ttcattttgc tgcacttttc gtaccctttt ttccagctca cgataagaga tatattcttg
     2461 ctgataactc tttttcttct gttggaaagc tacatctcca aaacgatcca ataaagcaag
     2521 atgtgattca ggctggagca gttcctgatg ttcgttttgt ccttggatat ctactaaata
     2581 actgccgatt cttctcagat tagctaatgt gataatatga ccattcactc gacaaacatt
     2641 ttttccagtt aaggacatat cccgtcgaac aatcagattg tcctcatctg tttcgatccc
     2701 taattcaacc atcagttcag agaatccttc ttgttttggt agttcaaaca acccttcaag
     2761 cacacatttt tctgctcctt gacgtatata atcgcttgaa cctcttcctc ctgctaacag
     2821 acctaaagca tcgataataa tggatttacc cgcaccagtc tctccggtca gcgcggtcat
     2881 tccttcgtga aaagaaagcc gcaattccgg aataatcgca aagttagtaa tactgatttc
     2941 ttgtagcatc acattcgctc ctcaacttaa aggtaatgca ggaagtcttt cttcaataaa
     3001 actgcctggt cttcagttcg tgtaatcatc aaaacattat cgtcatcatt gataatcgaa
     3061 aataattctt tcttgtaacg tttatcaatc aaatttgctg ctgccgaagc atttcccggc
     3121 aatgttttca aaataacaaa tttttccatt tggtcaactg ccacgaaagc gtcttttaat
     3181 agtttttcca atttagtatt tacatcttcg tttgtttcag ctggtatgct ataacggtag
     3241 ccgccatctg aagccggtac cttgatcagt ttcagttcct ttatatctct ggaaatcgta
     3301 gcttgagtaa cagatattcc tttgcttttc aggatttcaa caaattcttc ctgctttcgg
     3361 atatcttgct cactcaacaa tcgagtaatc aaacgatgcc gatctttttt tctcattacg
     3421 tcttcgctcc ttagcatatt cttcttaatc aatgatagct tatttccttg gtataataaa
     3481 gaagaatccg catacttctc acttgatttt cacagttaca atttatttat ggtcaaactg
     3541 ttcgtgtgca tttgaaacca cttctgccat attaatagct gaattgattg ttccgctttc
     3601 gggtactttc ctcaagtgag ccaagaactc gatatttcct tctccccccg tgatcggaga
     3661 ataacttaat tccagcacat catatccacc ttgtgccgca aatgacaaaa tgtcttctaa
     3721 aactttttga tgggtctgag ggtcacgaac aattccgttt ttccctacag cttctctgcc
     3781 tgcttcgaat tggggtttga ttaaagcaat cacttctccc tcttctttta aaatagcatg
     3841 taatggaggc agcatcagtt ttagtgaaat aaaagaaaca tcgataacag taacttctgg
     3901 aattcctaag gtaaagtctt cgggttttgc ataacgaaaa ttcactcttt ccattacgac
     3961 gactctagga tcttgtctta ttttccaagc tagctgattg tagccaacgt ctaaagcata
     4021 actcattttt gcgccatttt gcaaagctgc atcagtaaat ccgccagtgg atgcaccaat
     4081 atctaacaat gtttttcctt caacagatag atcaaatgct ttgagtgctt tttctaattt
     4141 taatccccct cgagaaacat aaggaagttt tttacctttg actcttaatt cactactcac
     4201 tggaattttt tctcccggtt tatcaaaccg ctcgtttttt tcgttgtaaa tcaaaccagc
     4261 catcactgaa cgtttggctt gttctcttgt ttcaaataat ccttgtttga ccgctaatac
     4321 atctacgcgt tctttttcca ttctttccct catttctcca catcaaattg cttgatcatc
     4381 tggcgcatca gcccgccatc aaaagaaaaa acgtcctctt ctaatttgtc tatgatcttg
     4441 ttggcggata acagctcaat ctccaacgcc tcacgtgtct tttctaggcc taataaacgc
     4501 gggtaagtat ttttttccat acgttcatct ttaccagctg ttttccctaa tgttttcgtt
     4561 gtaccaatca catcgagtag atcatctctt atctgaaaag ctaaacctaa atgttcggct
     4621 aatcgttgaa gtagcaataa gatttcttca ggttgcttcg ctaatatgcc accagccagc
     4681 aaggcataac gaatcaaatg ccctgtcttt ctttcatgaa taaatgctaa ttcttctaaa
     4741 gaaagctttt tcgattcgcc ctcgatatct gcagcttgcc cagcaaccat accttgtgat
     4801 cccgcacaaa cagctaattg ctgaagcagc agaagcttag gagaattacc taagtgtgcc
     4861 atactgatta gctggaatgc tcctgtaagc aaaccatctc cggccaatat agctaatgct
     4921 tcgccgaata ctttatgatt ggtaggtttt cctctacgaa gatcatcatt gtccatcgca
     4981 ggcaaatcat cgtggatcaa ggaatacgta tgcaccattt ctaaagcagc tgctacttga
     5041 tagacgggaa catcaatagg ctcatcaaag gaagcaacgg ccgttaaaag taataaggga
     5101 cgtattcttt tcccgcctgc tttcactgaa taactcatcg catcatagag tcctctatcc
     5161 gttgtatgct catctaaaaa agacaagatt tctttttcta cagcaggtag atgaatagct
     5221 gaaaagtcag ttaatctcat ctcagctttc ctcgtttccg tcaaaatcaa cttcttcatt
     5281 tgattctgtc atcattttcg tcaaggtctc ttctgcttta gctaatgtat cttggcaatg
     5341 tttgcttaat tccattcctc gtttaaacga atctagagct gattctaatg gaacgtctcc
     5401 ttgttctaac tgcatgacaa ttttttctaa ttcttgcagt gactcttcaa aagttggatt
     5461 ttccatattt ttattctcct ttgtctatct cttgtacttg tcctttaacg attccatctt
     5521 gataatggat cgtcagcaaa tccccttgat gaatctcggt gacacttttt actacttgat
     5581 catttaacgt cgtatagctg tagcctcttc ccatgatttt caatggactc aacaaatcaa
     5641 gagattgaac agcttgagca aaacgctgct gctgttgttt catatattgt tggattttct
     5701 cttctaagcg tttttctaga taagcagttt cttggcgagc tgctttaact cgataaagcg
     5761 gagccacttg ttccaattgg tggcttaatg cgatcgtttg tttttctttc gcatgataaa
     5821 tttgctgtgt agcttgatat aatcgctgtt tcaactggtc taatttgatg gtctgacctt
     5881 catacaatct ttcaggctgc cgaaatacat aggaacgtcg agcacgctcg aaccgttctt
     5941 gtttgcgctg gatctggcgt acaaacgcct gctgcaatct agcttgccgt tcttgaatgc
     6001 gcatcaactc ttctgacaaa acaggaacag ctaattctgc agctgctgtt ggagtggcag
     6061 cccgaacatc tgctactaaa tcagcgatcg tggtatctgt ttcatgtcct acagatgaaa
     6121 tcaccggcgt attagcttga taaatcgccc gagcaacccg ctcttcatta aacggccata
     6181 aatcctcaat cgaaccaccc ccacgaccga tgatcattgt atcgaaattg cccatctctt
     6241 ctactctctg gatattcctg acaacattgt ccgctgcttg gtttccttgt acaactgttg
     6301 ggaacaaaac caattggaca attggatatc ttcttttcac cgtcgtgatg atatcgcgga
     6361 taaccgctcc gcttggactg gtaaggaccg cgattcgctt tgggaaacgt ggcagcattt
     6421 ttttaggccc ttgaaaaagg ccttccttca tcagcttttc ttttcgttct tccaattctt
     6481 gatataaagc acctattcca tcaggctcca tgtgctctac atagatttga taagaacctc
     6541 ccgcttcata taatgaaata cgcccaacga caaggacttt cattccttct tttggctgga
     6601 atcgtaattt ttggaatgct cctttgaaca taatagcaga tatttttgca tggtcatctt
     6661 ttaagctgaa atattgatgg gcattggcac gtaaacggaa attcgatatt tcgcctgtta
     6721 aatatacgcg ttctaaataa ggatcggcat caaattttct tttcaaatac ttcgttaatg
     6781 tagtgacagt taaatattct tgtgccatct attcacctgc tccaaaagct tcggcagatt
     6841 ttacggtttg atacatcagc atcgtgatag tcattggccc tacacccttt ggcacaggag
     6901 tgatccagcc tgcaataggc tccacttcat caaatttcac atcaccgatc agcttaccgt
     6961 tagcatcacg attcatccct acatctatca ctacagcacc tggttttacg aattcttttg
     7021 tgacaaaatg tccacgtcct attgctacga ctaaaatatc tgcttcttgt gccagttcag
     7081 ccagattttc ggttttagaa tgagcaacag taacagttgc atctgccatc aagagtagct
     7141 gagccatcgg tttaccgacg atattgcttc gtccgataac tactgctcgt ttacccgccg
     7201 gatcgataga ataagcttca aacattttca tgatgccgta tggagtacaa ggaatcttgt
     7261 ccggtttacc cgcaaggagt ctgcctaaat tcatcggatg aaaaccgtct acgtcttttt
     7321 cagggcggat agctaacagg atcttttctt catctatatg ctttggcaat ggtaattgga
     7381 ccaatatacc atgaaaacta ggatcttgat tgtatttttc aatttctgca agtagttcgt
     7441 cttcagaaat cgtctcagga taacgctcta ccaatgaatg gattccgatt tcttttgcta
     7501 cacgttcttt atttcttaca tagatttggc tggctggatt ttcccctacc aataaaacga
     7561 ctaatccggg atggatacca ttatctttca atttttggat tttttcagca atctctgcct
     7621 gcattttttc agctaattct tttccgttaa tcaattctgc catctaacca ctcccagata
     7681 ttttttgtgt tttcattcta ccatagaatt ttcccgattt ataacaggtt tatgatgatt
     7741 catagaaaaa agctaggact tacgccccag cttctaattc tttcaataca ttggataaaa
     7801 caccgttgac gaatttacgt gaacggtcat cactaaatgt ttttgccaac tcgattgctt
     7861 catttaaagc aacagccgct ggtacatctg tcacataagt catttcaaaa atcgcgatac
     7921 gcaagatcac caaatccatt tttgagatcc gattgatgtg ccaattgttt tttaaatgtt
     7981 tttcaatcac tttatccaat tcatcttttt ttgcacagac accgccaact agcgtatcaa
     8041 gataaacagg aacaaactct gattcatctt cgttgatcat gtcgcggtgg tctaattcga
     8101 ttgcgttgaa aatcgcatct tcttttgtta ggtctgcatt gaagtctaat ggaaataaag
     8161 cctgcaatgc catttcccga atctcgtgtc ttgataattc tttattcatt ctcttcctct
     8221 tcgtcttcaa aaagttcttc gattgttggt tgttctaatt tttcaggaac aacagctact
     8281 acatggatat tgacttcaga taaatcgata tccgtcatga aaaggacttg ctggctgacc
     8341 cgttcctgca tttccattgc tactttagga actgaaacac catattctag ataacagtaa
     8401 agatctactt tcaagccatc ttcctcgttt gctaagtaaa caccttttcc gtgagctgca
     8461 cgtcctaata attcagtgac attgctggca aaagttccgc gcattccgta tacgccttct
     8521 actttagaag ctgcaatccc gataattact tcgatgactt caggagcgat cacgatctct
     8581 cctaaatctt ggtttgcgtt taaaatcaaa tttttttcat ctgccattta tttattcctc
     8641 cctttgccta agcacgagat acatccgaat tctctatgat gcggcatagt gatcacgtgg
     8701 taagaaaact gccgccgatc agctgcaaac tgctatgaat tggagaaaac acccaacttt
     8761 attatctctt acagcttaaa agttcttcta tagtgtatca ttaaatcgag tgattttcta
     8821 taacaaagtc tgtgaacact gatttttacg cttacttctt ctattgatga aactataaga
     8881 tgatcaattc ttttggtgaa cgagtcaaca cacgatttcc atcttctgtg atcaacaagt
     8941 catcctcaat acgtactcca ccgattcctg gtaagtagat ccctggttca tcagtgatca
     9001 catttcctgg tacaaattgc ttttctgcac gagaagaaac gtttggtcct tcgtggatct
     9061 ctaaaccgat cccatgtcct gtagaatgtc caaatgcttc cccgtaacca tgttttgaaa
     9121 tataatctct agcaactgca tctaattgga caccagtcat tcctggtttt gcagcatcaa
     9181 taactgctaa ttgagcatct aagactattt gataaatttc ttttaatttt tccccaggat
     9241 caccaatagc gaatgtacgt gtcatatcgg aaacgtaccc ttcgtaatag caaccaaagt
     9301 ccaatgtgat caagtcacct tgttcgatga tttttttgct tgctacacca tgcggcatag
     9361 ccgaacgcaa tccgctagca acgatcgtat caaaagaaac acctgaagcg ccaagagaac
     9421 gcatataaaa gtctagttga tttgcgactt caatctcagt catgcccggt tggatcattt
     9481 tcaggatgtg atcatatgcc atatccgcaa tatgacatgc tttttcgata atagcgattt
     9541 cttcttcatc tttcacttct ctgagttctt cgatcatctg tgaaacaggt actaattccg
     9601 catcgatgat ttcttctaaa accgaatatt ctaaaaatga gactgttgtt tcctcaaaag
     9661 ctaatgagtg cagttcttct ttgcgtacta agtttgctac ctcatcaaaa ataggtccga
     9721 catttttaat aatttcaaac ccttgagcct gcgctgccgc ctgctcggta taacgaaaat
     9781 cagtaataaa aaatgctttt tccaaagtaa taacagctaa tcctgtcgta cctgtaaagt
     9841 tcgttaagta acgaagattg tacggactgg taaccaaaaa tgaatccaaa ttttcttctt
     9901 gcattttttt gcgcaatttt tctacacgta gcatcataat gaaactccct ctttctttgc
     9961 acgctttttt ccattatagc aaacttttct tcgtacacga cagttaaata aaaatttccg
    10021 gaaaacttct ttttat
//
LOCUS       DAIGOK010000101         9974 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.102, whole genome shotgun sequence.
ACCESSION   DAIGOK010000101 DAIGOK010000000
VERSION     DAIGOK010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9974
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.102"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            166..360
                     /locus_tag="NOA01_002088"
     CDS             166..360
                     /locus_tag="NOA01_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633271.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            629..928
                     /locus_tag="NOA01_002089"
     CDS             629..928
                     /locus_tag="NOA01_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633272.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1025..1459)
                     /locus_tag="NOA01_002090"
     CDS             complement(1025..1459)
                     /locus_tag="NOA01_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCI0633273.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1691..2401)
                     /locus_tag="NOA01_002091"
     CDS             complement(1691..2401)
                     /locus_tag="NOA01_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCI0633274.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2391..3251)
                     /locus_tag="NOA01_002092"
     CDS             complement(2391..3251)
                     /locus_tag="NOA01_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HCI0633275.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3256..3894)
                     /locus_tag="NOA01_002093"
     CDS             complement(3256..3894)
                     /locus_tag="NOA01_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCI0633276.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3909..4208)
                     /locus_tag="NOA01_002094"
     CDS             complement(3909..4208)
                     /locus_tag="NOA01_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCI0633277.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4251..5735)
                     /locus_tag="NOA01_002095"
     CDS             complement(4251..5735)
                     /locus_tag="NOA01_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCI0633278.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5755..6216)
                     /locus_tag="NOA01_002096"
     CDS             complement(5755..6216)
                     /locus_tag="NOA01_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCI0633279.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6234..7301)
                     /gene="ulaG"
                     /locus_tag="NOA01_002097"
     CDS             complement(6234..7301)
                     /gene="ulaG"
                     /locus_tag="NOA01_002097"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCI0633280.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7519..8289
                     /locus_tag="NOA01_002098"
     CDS             7519..8289
                     /locus_tag="NOA01_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCI0633281.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            8641..9936
                     /locus_tag="NOA01_002099"
     CDS             8641..9936
                     /locus_tag="NOA01_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HCI0633282.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT         3122 a         2042 c         1633 g         3177 t
ORIGIN      
        1 agaacaatag ccttttatta tatatattat tcaaattagt tatatcgagt gaaaaaaact
       61 ttcaaattga ttctttttat ttatcataaa gatatagata cataatcatt ttcacttaca
      121 tatatttgaa tattaactaa aaacagcaag aaagaagagg atcaaatgta ttatgtagaa
      181 gtaaaaacaa aaggcgtcaa aaacaaacaa tatgtgaaag gtatcagtaa cgaatatcct
      241 ttacttggtt catggaaaga agctgctcct ttttccaaac catgtgccat aaaaataaag
      301 aacgagctag aaaaagaact gacttgtggt aaagctgttg tagatatcat cgaaaagtaa
      361 atccaatgta tagaaaggaa gatcacacat gaaaagcgac gtacaaattg ctaagattat
      421 agaattagaa gaagaagacg cagtgttgac aattgaaaaa aaataaatgt ttgtatacca
      481 tatgattatt tcaatatgta tcctggaatt ggagatctcg tagagctata tcaagatgaa
      541 tcaaatatac ttgtcattca tcatgaaata tagagactta taaaattgtt tatttttata
      601 atgtatcagt atagaaagga aataaaacat ggattatact gttgaatttg acagtgaact
      661 acttaagcaa tttccacaag aagaacattt catgtataaa gaagcaattt cccacaaagt
      721 gacagagtat ttggagcata atttttttcg tataaagcca gtgcgtactc gttcggcaaa
      781 aggaatatac gagatgaaag tcaaaattaa taaaaaaaat tatcgcatag catttgcttt
      841 aaaaaaaaat catgtgttca ttttttatat tagttcgaat ttgcaaaaaa ttgtatttga
      901 taaagaggtg agtaaaaaaa taaaatagct gagcattgga ggatagattg agtgatacta
      961 agtctatcct cttgtttttc taaagaacta agctagaatt ttttcttttg tacgttatta
     1021 tatgttattt gtcattacca aaaagcacct tttttcttct agtatctatg acataagaaa
     1081 taccacccat ttcctttact tgaacaaatc ctactgattc ataaaatttt tttgttttct
     1141 cagtatcatc tgtcaataat tcgatttgga ctggtccata actagtgatc ttctccaaag
     1201 cttcattcaa aagcactttg cctattcctt ggcgctgata cctcggatga atgaggatat
     1261 cttgaataaa tgcgatcgtc gcaaaatcag tgatccaacg aacgattccg ataatcttcc
     1321 cttccccatt acgctttatc agagattcag attgttcaaa tgctctagct aaccttgcag
     1381 tgtcttttgt gtaattcgtc caaccaacgc tcttatataa ttcagaaatc tcatcgatca
     1441 taagggaatc aatattcatt tttttcttcc tttcttatac aattctccct ctaataatac
     1501 agttctttga ctatttaatc tatcacatat aactctcctg taaagattct ctcgattcct
     1561 taaaattcct ctttcctttt agaatgcata ctttttgata aataaaaagg ttgtgataaa
     1621 ttttcttcac aacctctgtt tctctctatc aagtgttatc ttttaatttt ctacccttac
     1681 cctagcatct ctattgcccg tagtatgcat actttccatg tttgcgaaaa taatgtttat
     1741 ctagtaaata ttgcgggatt tcacatatat tggggttgat cagctcgttt tctttggcca
     1801 tcagacaaat ttcatctaag atcaaactgt tttctactgc tttcataggt gaatcccccc
     1861 atgtgaacgg tccatgtcca tagacaagta ctccagggac ttcgtttgga tctaattttc
     1921 tttccttaaa cgtctcgaca atcacattcc ctgtatgaac ttcgtaagct tctcttactt
     1981 cttctttcgt taattggcgt gtacaaggaa ccttaccata aaaagcatca gcatgagtcg
     2041 tgccataagc tggcaaatcg cgtcctgctt gcgcccacat gacggagtgc gtggaatgag
     2101 tatgagtaat agcagttata tcttcaaaag tttgatacaa aacaacatgt gtggggagat
     2161 ctgaagatgg ttttaagcta tcttcttcta ttatatttcc cgacaaatct gttaccacca
     2221 tttgatctgc ttgcatgcat tcatatctga caccgcttgg cttaatgacg atgactccta
     2281 gctcacggtc cacttcactc acattgcccc aagtcaattt gactaatccg tatcgcggca
     2341 atgccagatt tgcttcaagg acacgttgct tcattgctgc aattttttct tcacaagaca
     2401 tcatagccag cctccttcaa tttagaactt aagtagtctt ttgcctgctg gatttctctt
     2461 cgaaagtctt ggcttttttc actccacatt tcaatcagaa atgttccatc ataatctaga
     2521 cgtttcaaat gtcttaataa tccaagaaaa tcaacacaac cgctaccaaa aggaacatca
     2581 cgaaactggc ctgaatattc ttttgttaca gggtaagtat cttttaagtg gatagcagtg
     2641 atataatcga tccctttctc aagttctcga gccggattat tttccggcca tgcggacaaa
     2701 ttgcctaggt ctggatatac ttgtagaaaa ggtgaatgaa tctgttcttt gatttctaaa
     2761 aattttgaaa tggagttcat aaatggatca tccatgattt ccacggaaag aatgatgcca
     2821 tatttagaag cttcctttac gctttctttt attccttcga taaagtcttc tctggaattc
     2881 attgatttct cttcataata aacatcgtat ccagctattt ggattacttt gacgcttaga
     2941 ttatgcgcca aatggattgc ttttttcatt agttgtttcg ctgctttttt cttttctgga
     3001 tcagcagaac caaacggaaa tcgccgatgc gcacttaaac aaatcgaatg gatacgtaca
     3061 ccggagctga acatctcttc tctcagctca gctatctttt cttcagacca atctaatcgt
     3121 gctaaccgct catccgtctc atcaatcgac atctcgataa aatcgaagtc catttctttt
     3181 acaagggaaa aacgttcttt ccatgaaata tctttgggta acgctttttc ataaatccca
     3241 atttgagcca tttccttacc cccagatacg aatgatctca tctttgaagt tttgtgcggc
     3301 cttcatcgga tcatttgctg ctgtaatacc acgaccagtg ataaaggtat acacatcgat
     3361 cccttcaaat aagtgtaatg tgtgtggatt taatccacca gttacagaaa cattaaagcc
     3421 tagttcaatc agttttttga tcttcgataa atctttttca ccccaagttt caccagataa
     3481 cagcgcatca cggctttgat ggtagattac ctgccggata ccgatgttat gccaatccat
     3541 ggcttgttca aaagtccaat tgccgtagag ttctacttgc aattcaccta tttctttttg
     3601 tgctgcctcc atagttggaa tcgttgcaca acaaatgact gtcatccagt ctgctcctgc
     3661 ttttgcaaca ttactcgcca ctgttcctcc agcgtctgca catttcgtat cagcgactag
     3721 ttttttatct gggaacatac tgcgaataca gcgaatcgct ttttgtcctt cctgtaagca
     3781 aagaattgtg cccacttcaa taatatctac tacatcacca actttcataa catctcctaa
     3841 tgcatgttct aagtcattgt gatctaacgc aatttgcaaa ttcggcctgc tcatcttagt
     3901 tccttccttt acttgttttt ttggtaccac tttgtttcag caaatttttc actgatttct
     3961 ttgactgaca tcacattttt gataccgata actgtaactc cttttttctc tgcctgagta
     4021 aacatattca aaaaattgac tggcgtaaaa actacatcgt attggccagc tgaacttttt
     4081 ccctctgaaa tggcacaatg atggattttg gtaattggta cgtttagttc tttcattgct
     4141 ttttccacac ttctcatcat catcaagctt gttcctgatc catttgcaca cgatactaaa
     4201 attctcatct taatccctcc attatgagtg ttgtctaatc agattttctt ttatactctc
     4261 tgcttttctg catatgtgtc atagtcttct acaatcaaaa aataaccatc aggatttttc
     4321 cgatattgta actgcggaat agccaataaa ccaatgacaa ctacggcaat tccagcataa
     4381 cctaggactt tcatgattac tgtaaacaat ggccaagctg ttgcccagtc gaacatcccg
     4441 atataaccac cgtatgttga catcccaatc catgtagcga ataatgctga accgcctact
     4501 tgtatgattc ctgaaagaaa cgggaacagg caagcggctt tgaatccacc tcggttattg
     4561 gcataaactg ctataacggc attatcaaag aacaacggaa tgaaaccggc tatgacgatc
     4621 gttggtgatt tcaataaaat cagtaaacca atcatcaaaa actgtcccaa agcaccaaac
     4681 aagaacccaa tcgttaccgc atttggcgaa ccaaagccaa aagtagcagc tacgtcaatt
     4741 cctggaacag ctcctggtaa aatcgtgttt gagattcctt ggaaagattg agttagttca
     4801 tcgacgaatg tccgaacacc taattgcaag atcgccagat ataccgcaaa atttaaagaa
     4861 gtcgtcataa tgtagaataa gaaactctgc ccttcttgca tgaattgcgc ttgaatcaaa
     4921 taatctttgc ctagtacaat cagaatgatc ccaaagaaaa atagcatgag taacgaagta
     4981 gcaaccatat tctcattgaa aatcgacaag aatcctggta atttgatatc ttcaattttt
     5041 ttgtcttgct tgattgatct atttttcttt tcatcccgct ttttcatcca ctcagctaat
     5101 tttgcaaata taaagattcc gaacatttgc tgatgtgcca ctgcaaatcc ggcaccttct
     5161 gtcaattcct gcgtgattcc tactgttaag ttagaagcta ctgtccaata acaacctaaa
     5221 atcagtccca taaaaatcaa tgcttgtatc ctacctagat caggaaagca aaacagtaaa
     5281 atccaaaaag cggtagctgc ttgttgaacc tgaacgtttc cggtagtgaa aacggaacgc
     5341 agtttcgtat atttctgaaa acggactaat aagatattta aaacaaatgc aatcagtaat
     5401 aaaatcatca catctccaaa ggtacggcca aatgtctcca ttaatccagc atccacagca
     5461 ttttgtccaa aataaggatc tgtaaccatg gcatccaaat tgaaccgttc ctttagtcca
     5521 accaagattg gccgaaaatt attgaccaaa ccaccagagc caacagtcaa aataaagtaa
     5581 ccgaccgttg cctttaagaa tccagcgatg cattcatgaa gcgggcgttt cagcaaaata
     5641 tagcctaata acacgataaa accaatcagg tatgctggct gcgtcaagat atttgttgca
     5701 aaatacgtcc aaatgcttaa caaaaattcc cccatctttt ttctcctcta tcaattaatt
     5761 atattctgcc atcacaactt ggtaatcatc gatggagttc gtagagatga gacgctcaac
     5821 caatccatct gtcattaata attcggataa tttttggata ttttctagat gttcttctgg
     5881 atcttttgct gcgagtgtaa aaaacaaact agccgttttc tcctcattct ccccttcact
     5941 ttcaaaacgg attggctgtt tcaacttggt aaatgaaata gctgttccaa aaacaccatc
     6001 acttttttct gaagaatgcg gcattgctac ttgaggtgcg atcacaatat aaggaccgta
     6061 tttttctaca caatggatga tctcctccac atacgtttca tcaataattt ccttatcgat
     6121 caatacaaga cagctttcgg cgatagcttc acgccaatcc gtgatttctt tatctgggta
     6181 atgaatcaat tgatggtcaa aaaagtattt cagcaagaaa ctccccttct ttcttataaa
     6241 aacgacggaa acggtgtatc attttcaatc gagaagacat cttcaaatcc tcgataaaaa
     6301 ttaaactcaa gatcatcttt gtttgtcgga tagatgaatt tcccgcctac ttgccagata
     6361 taaggtttaa atccatactt caggcgatcc tttttgaatt tccaaatttc agtgatctct
     6421 tttggatcag ccataaaatt agaccaaata tcgtagtgga caggaatcac tacttttgca
     6481 ttcaatgact ccgccatgcg cagcatatca acagaagtaa ctttgtctgt tatccctcgt
     6541 gggttttcac cataggcccc taaacagaca tcgattttgt gttcattacc atgtttggca
     6601 aataaattgg agtaatgtga atctcctgca tgataaagat tccctcctga tgtttcgaat
     6661 aaatagttta ctgcaatttg atccatctcc tgaggcattt ttccttttaa tacctcttct
     6721 ggatcttcac aagtgaccaa cgctgtccga tcaaacgctt ctaaagctac tacattgatg
     6781 tctttgattt tgactgcatc acctggcttt actgtaatcg ttttttcttc aggaattccc
     6841 catttttgcc acgtgcgaac cacttctttt ggcccaatga atgttgcttc aggacaattt
     6901 tgatgaactg ctgcggctgt atggatatct agatgatcgg aatggatatg tgtaacgatt
     6961 aaagcgtcta cattttttat cgcaaaagga tcgattacga aaggctgagt acgtaaatta
     7021 ggctgcatat tttgaacgcc actcatacgc atcatctgat ggcctttttt catcatgcca
     7081 ttcccatgac tacgttttcc cgtcccacac catagatcac ataaaatatt cgttccttca
     7141 tgacttttca gccaaattcc cgtacagccc agccaccaca tagcaaaagt tccaggatcc
     7201 actacttctg tttcgatttc ttcattcaag tatgtgcccc actcaggaaa tgtttctact
     7261 aaccactttt cttttgttac ttcattgaca gttttactca tttgcttgct ccccccatat
     7321 cgttgttcca acttacaagt aaatattaat gtgtatcagc atccatgaaa acccttttat
     7381 atgaccgttt gttttttata tgattttctt tcagtaatca atgattgttt cagtataata
     7441 aagacagata tgaatgttta aatgaacaac tagtcaaata aatccatgga tcaagatgtc
     7501 cctcaggagg caaaagtcat gaaaaactca cttgtcaata ttgaaaaacg tcaaaaagaa
     7561 attttggaac tgcttcagca gactgaacaa ctatcaactt tcgaattagc tgaatccctt
     7621 gatgtatcta ttagtacgat acgaagagat ttaaatttat tagaagaaaa aaacgagatc
     7681 atacgcaatt acggttattg ttcttataat tacaaaaacc agactgattt tgatcaatct
     7741 ggtcctgaaa ggattaaaca agcgattgcc agagaagcta gtaaatacat taacgattat
     7801 gatacgctgt ttatcaattc tagctcgact gcgttgaaaa cactggattt tcttcaatct
     7861 aaccatttga cagtcattac aaacaacgtg aaaatcaatt actctccgca caaagataat
     7921 tgtagttata ttttaactgg tggagagatg cgctttccaa aagaagcact agtgggcgac
     7981 atcgcattaa atacgatttc ttcagtcaat gcagacattt gtatcattgg ctgtagcgga
     8041 gtcgatgtag tacatggggt cacaacaaag atattaaatg aatcaacgat caacgaaatg
     8101 atggtcaaca agacaatcaa atgtaagatt ttagtcgctg atcatcgaaa aatcggactg
     8161 acatcaaagt tcaagattgc tgatatttca gtctttgatt atttgatcac tgataaattt
     8221 tgttctgaaa aagtattaga ggagatcaga aatacgggga taaaaaccat tcaagtagat
     8281 tcattttaag aaccaggacg taaatatgta aataggatta tcaaaaaaat gaataacaaa
     8341 tttatcgata aatgaaaaac acgctacgct caaattgata aaggaatccc aagatgtatc
     8401 catccaatcg ataccatcat aatctattgc ttcttttata accgttatat gttgcctaaa
     8461 taggaatcaa acaatatcga aaaaaggagg tatgacaaaa gtcacacctc cttttttcgg
     8521 ctctatactt tttgatgttg gtgaacttca ccaacatctt ttttgtataa aaaaatatag
     8581 aaaagacacc ctgaaatcct ttagaattaa tttggctacc aattcatagg aggattcaag
     8641 atgtctcata ctcagcttat caaagatacc ctaaatattc tagacctaaa tatctatttc
     8701 gaggaaaatt gtttaactaa agaaaaatac aaagggcaaa tttgcttgat ttacaaagga
     8761 actcttctgt acaagccaga agagtgttcc cattgtcttt gtgtcgtccc ctctaggatc
     8821 attcgttggg ggacgacaac cgttcggttg ttactgaatg atgtgtctga gtatcgcact
     8881 tatctggagc tgaagaaaca acgatttaaa tgtaagtctt gccaaagaac attcgtagcg
     8941 gatacctctg ttgctgaaaa acactgtttc attagtcaaa aagttcgttg gtctgtcatt
     9001 gctcgcttaa aagagaatac ttccatgacg gaaatcgctc gtcagaaaaa tatttccact
     9061 tcttcggtct atcgtgtcat gaagcgattc taccgcccat tgaatccttt caaacagact
     9121 ttgccaaagg tcttatgctt tgatgaattc aaatcggttc gaagtgttgc cagtgcaatg
     9181 agttttatca tgatggacgg acaaagccat gcattattgg atattgtcga aaatcgacgc
     9241 ttgccttatt tagaacgcta tttttctcgg ttttcattag cgactcgtga agccgttcaa
     9301 ttgattgtta ttgatatgta tgcgccttat gtttcgttgg tgaaaaagct tttcccgaat
     9361 gctcagttga tcatcgatcg tttccacatc gtccaacata ttggcagaac tttcttgaat
     9421 catcgagtga aagaaaccac ggtacgactt aaaggaaatt cccactctca acgagggtta
     9481 gggaaaaaat tgaagcgcta ttggaaactc ctacaaaaag aagaagcaaa gttaaactat
     9541 gaaaaacggg tttggcgtgc cagttttaaa gattatttga ctgaaagtga gattgccgat
     9601 cgactgttag ctgcttgtcc caacttacgc caaggctatc agttatacca agacattctt
     9661 tatgcggtaa agaaaagaga tcagtcacta tttgaggatt gtttaactag agacattagc
     9721 ggtctgccag aaacctatac aaccacttta cgaacgttta aaaagtttct gccacagatc
     9781 caaaatgcgc ttcactacag ctattcaaac ggaccattgg aatgcttaaa caaccacatc
     9841 aaagtattaa aacgcaatgc gtacggcttt cgcaactttt acaatttcaa actacgaatt
     9901 atgattcgtc atggcaaaac atttttaacc aaataaacag aacagaaaga ggttttcttc
     9961 tttctgttct gtcc
//
LOCUS       DAIGOK010000102         9841 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.103, whole genome shotgun sequence.
ACCESSION   DAIGOK010000102 DAIGOK010000000
VERSION     DAIGOK010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.103"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            341..1339
                     /locus_tag="NOA01_002100"
     CDS             341..1339
                     /locus_tag="NOA01_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCI0633283.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1451..1960
                     /locus_tag="NOA01_002101"
     CDS             1451..1960
                     /locus_tag="NOA01_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCI0633284.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1989..2648)
                     /locus_tag="NOA01_002102"
     CDS             complement(1989..2648)
                     /locus_tag="NOA01_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HCI0633285.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2650..3414)
                     /locus_tag="NOA01_002103"
     CDS             complement(2650..3414)
                     /locus_tag="NOA01_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HCI0633286.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3422..4099)
                     /locus_tag="NOA01_002104"
     CDS             complement(3422..4099)
                     /locus_tag="NOA01_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HCI0633287.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(4136..5521)
                     /locus_tag="NOA01_002105"
     CDS             complement(4136..5521)
                     /locus_tag="NOA01_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCI0633288.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5731..5815)
                     /gene="ffs"
                     /locus_tag="NOA01_002106"
     ncRNA           complement(5731..5815)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="NOA01_002106"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5838..6353)
                     /gene="tadA"
                     /locus_tag="NOA01_002107"
     CDS             complement(5838..6353)
                     /gene="tadA"
                     /locus_tag="NOA01_002107"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCI0633289.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6460..7317
                     /locus_tag="NOA01_002108"
     CDS             6460..7317
                     /locus_tag="NOA01_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCI0633290.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7398..7733)
                     /locus_tag="NOA01_002109"
     CDS             complement(7398..7733)
                     /locus_tag="NOA01_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633291.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7800..9170)
                     /locus_tag="NOA01_002110"
     CDS             complement(7800..9170)
                     /locus_tag="NOA01_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HCI0633292.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9248..9784)
                     /locus_tag="NOA01_002111"
     CDS             complement(9248..9784)
                     /locus_tag="NOA01_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCI0633293.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
BASE COUNT         2899 a         2027 c         1640 g         3275 t
ORIGIN      
        1 tgtgatagag aaataacaat ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat
       61 ttgattttga tcgggtctag tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc
      121 ttaaatttca tcttgactta tcaccattag actaatattt gcatacttca taataataat
      181 aacataatac ctaacctctc gtaaaaatac tagatttgtt ttcttttatt tttcatcact
      241 agatacattc tataacttac cgtttcgatt tcgaattcaa acatttttga gaagcattct
      301 caattaaatt ctgctatagt gtgaaaaggg aggaattttt atggaaatct atgacattac
      361 aatcatcggt gcaggtcctg tagggatgtt tgccgctttt tacgcaggaa tgcgccaagc
      421 taaaacaaaa atcattgaca gtctacctca acttggtggt caattagcaa ctttgtatcc
      481 cgaaaaatat atttacgata ttcctgggta tccagcaata aaagcaagtg aattaatcga
      541 tcaattagaa aaacaattaa ctacttttaa ccatacattc catttaaaag aagaagttct
      601 ctcgcttact cgtgaagacg aagttatcga gattactaca aataaaggca tccattattc
      661 caaagcagtc attttagctt taggaaatgg ttcattccag ccaagaaaat tgaatttaga
      721 taatgctgaa tcatttgaaa accacggatt ggattatttc gtgaatgatt tgatgaagta
      781 tgctggaaaa aaagtcgcta ttgctggagg tggtgattct gctatcgatt ggacgttgat
      841 gttagagcct attgcaagcg aagtttactt gattcatcgt cgcccggaat ttcgtgcgca
      901 cgaacatagt gtatcacgct tgaaatcttc atctgtcaac ttgcttactc cctatctcat
      961 cgatggatta tcaggcaata atggggaact gacagatatc cggttaaaaa aagtgaaaag
     1021 tgacgagacg attgatttga tgattgattc tctgatcgtc aattatggat tctcttctaa
     1081 tttagaacat ctttcttctt ggggacttga tagtaccaga aatgccatta ctgtaaaatc
     1141 agatatgtct acttctattc caggcgttta tgcagctggt gacatctgta cctatgaagg
     1201 aaaagtgaaa ctgattgcta ctgggctcgg tgaagcacct actgcagtga acaacgcttt
     1261 acactatatt aatccaaagg aacgaactca gcctggtcac agcacaagct tatacgataa
     1321 aaatcttcgt cctagttgat tttttatatc tctttgcatt tcaagttaaa cccgaaagca
     1381 gtttcttgca tttttttcat gttccgttaa catgaaatag agaaagaaaa aataaagggg
     1441 gagcaataat atggtcgtca aaactgagac tttagaagaa aaggcaagag aactattgaa
     1501 ggaacgtggc gtaactgtag aagatatcgc acaacttgtc atgtttttac aaaaagacta
     1561 tatttctgat ttgactttgg cggattgtac tgaaagtgtc aacagcgtgc taacaaagcg
     1621 tgaagtacat aacgccatta tcactggaat ccaattagat atattagcag aaaagaaaaa
     1681 attgatgagt ccgttgcaac atattatcga agatgacgaa ggcctttacg gtatcgatga
     1741 gatcctagcc ttgtcaatcg tgaatgttta cggtacgatc ggctttacga attatggtta
     1801 tatcgacaag gtgaaacctg gtattttaaa agaactaaac agccacgatg gcgttcatgt
     1861 ccataccttc ttagatgata tcgtcggagc gatcgccgct gctgctgcca gtcgattagc
     1921 acacaaagaa ccagaaaaaa gaagtcgcct tactcaataa ttcaacaaaa aagaaagaaa
     1981 acgtctattt agacgagttc tttctttttt gtttgtttca agctattttt tcctaagaaa
     2041 agaaaaacag caaaaaataa ttctaataat acaaaaaata agataaagct atgcatgaaa
     2101 aattcttctg gaagtacatt gatgattgga tcagtaacgg gatcgaataa ccagtcatcg
     2161 ttgttgaaaa acgtttcatg aaaaaggata aaaaagcgat caaatcctat catcatgaaa
     2221 aaaccgaaaa caactggcag taacatgccg atttgaaacg gacgaatcaa ccgccaaagc
     2281 cgatcattct tttgcaaata tttgataaac aaaatactcg ggatcaaagt gatgaaaaac
     2341 acagcataat ccactaaaaa caaaattttc acttcataaa agtgatgggc tccgctagca
     2401 gaaactggga aatctggcaa gctgaggatg gttttgaagg gattattgag ataattcatt
     2461 aggtgatcaa aatttttcag taacgtctct tgatctaaag aagtataatc caaaatttgc
     2521 aaatgtccga tatcaaatac gtatagtggt cgaaagttga tcgtcacacc aactgccaga
     2581 ctgatcagtg tcagaaatag gctgaacaat ccagcatatt ccatccactg ccatctcgaa
     2641 tttttcatat tagaagctcc attcatccag tgattgtttc acataggtag ctgggatagg
     2701 caacccaggt acatcttctt cttttgtaaa acctgataaa acgagcaaag tatctatatt
     2761 attgcgtatc ccagcttgga tatctgtttc atagttatct ccaaccatga ttacttcttc
     2821 tttttgcagc cctaatactt caacggcttt atccataatg atggcttctg gttttccgat
     2881 gtagatcggc ggcgtttggg ttgctgtctc tacaaaagag atgactgacc ctgctccagg
     2941 aagaaggccg cgttcagtag gtatattctt atctgggttc gttccaatga aagtagcacc
     3001 tttttgaata gcaagtgttg ctttaactac tttttcatag gtcagataat tatctaagcc
     3061 taccactaca tagtcaggag cctcttcttc ccatgtaaat ccggcagcta aaatcaagtc
     3121 aatcaatcct gcttctccga taacatagac ttttttccct tttccatctg ctttcataaa
     3181 atcgattgtt gccaaagtag ctgtgtagac tgtctctgga gctacatgaa tgtcaaactc
     3241 atctgctaga cgattagcta ctgtttctgg tgacttagtc gtgttattgg taacaaacaa
     3301 gaaaggtaat tttctttttt gcaattcttc cacaaatcgt ttaccggccg gtatgggttg
     3361 tgtcccacga taaatcgttc cgtctaaatc aattaaatat cctttatagt ccattcatcc
     3421 actactcttc cctttttctg atcgtaaatg ctgtttttgt tttttctact tgtttaggtt
     3481 gttcttgttt ttttactttt tcagaggttt ttgctgactc tgtttttcga tttttaataa
     3541 ccggtttttt gcgattagat gcgactggtc cttttttctc atcgatatac gcacgattat
     3601 ggtttttttt cttttttcgg cgctgagttg tcttttcttt tttaccgccg attctctcga
     3661 tgacaaaata cgcacaacca aaattgcaat attcgtaaag atagtcttcc aaggaatcga
     3721 ttcgctgatc tggatgcgcc cgtcgattat ccgcttggaa aaatcctttt aggcgtagtt
     3781 gttcataacc ccaatctcct acgatataat catatcgggc tagtacatca ctgtaacgtt
     3841 ctccaagctt ttctgcatta aatccttcac gataatcgga cacaagccga taatgtcttt
     3901 cacctatcaa aaaattactg tcatctaaca gtgtcactag ctcgcctttg actttttctg
     3961 cttttggctc ctcaacgatt tcttccaaag cagctgtcag ttcttcggtg actgttgttt
     4021 cctgttcagc tgatgatttt ttcttagcaa tttctgtttt atgctcaacg ttctgctgtt
     4081 cattttgttt tgcagacatg cgttcaccac ctatcttttt tattatacct tgtttttatt
     4141 tgattgggta gtgagctttc aagtaatctc ctaccacact tcggataaat tcagggaaca
     4201 aaaattcatt ttttccagca atctcaatcg ttggaaaaaa tggaacaaac atcaaatgat
     4261 ctacggtagt aaaagtataa tttttattca gatcgattgg cttgttcttc caaaagactt
     4321 gatggttaat agaatcatat gtgattccat tatagaccat ctgcccaaaa atcttaccgc
     4381 gaaatcccat tcccttcata ggatagttac gtaaaaagtt acgatttttt tctatttcta
     4441 aaactaagcg gatcagatca tttccaccca atgtgacatt taacaagtgc atcggatgcg
     4501 gcaacgcagt gtgaagctga tcctgattga cccgcccttt tggaatttca gttaaaaaca
     4561 gaccactgtt taatattgct gcttccgtct ttcctcgttc tttcaccgcc tcaaaggcta
     4621 cttggataaa cgaatgctca ttgaagatat ctagactcag agaaaatggc aaatctgcta
     4681 cttctttttc ttttaataat tcatgcccac gttctagata actatttatt tcttgttcat
     4741 cttctgtaaa agctgtcatt gtttcggtag ggatggcttt ggctgaatgc ttaaggattt
     4801 ttttctcttc atcgatggtt aaatggactt caccgacata ctgcccgtac tttccagcgg
     4861 ctgctatttg gacatcattg attacgtgac cttctctgaa taaatgatgg gtgtgcgatc
     4921 ctaaaataac atcaattgct ggtatttctt ttgcaatttg gaaatcatcc tgaatcccga
     4981 gatgactcat caaaactaaa acatccactt gtgaacgtaa atcttcgact aattctggca
     5041 aaatatcata aggattccgt atgtcccagc cattcggatt ataagtaagc ggaaatggag
     5101 cagtaaatgc aattagtccg attttcgtct ggtgtttcgt tgtaatgatt ttatatggtt
     5161 tcgtccatgc cggtggctgg agcgtatttt tatcgaataa gttatccagg atgatatcaa
     5221 actcagcatg atcgtataaa tgattcaact cttctttgga atttcctacg ccttcattgt
     5281 ttcctatcgt cgctgcatca taaccaactt cattcatcag caagatattt tcctgtccat
     5341 tcgttgcttc tgacaatgga tgccaacgat ccacaaaatc acctagatct actgcaatcg
     5401 ttgtcgtttt ttctttcttt tcgttttctc gtttacgttg atccaaaaac cttctgatcc
     5461 ttggccaatt ttctaaatga gaatgcagat cattcgtatg taaaatcact attttttcca
     5521 tgtaccctct cgcctctctt tatctttatc ataccatagg cagctttttc ttccattgaa
     5581 acaagctcta tttcagagtt tttcctgcct tgaagcaaat tgtataagta caatattcga
     5641 taaatcagtc tggctaaaaa atcaggaaca ttcattttta cagtactata aaaataaaaa
     5701 aaacagaccc gaaagtctgt tgtcgtttca ttataatcag acacatggcg gaacctgctt
     5761 agtgctgctt ccttccagac ctgacacgct tcacaagccc accattgtcc tagcctttcg
     5821 gcaaagaata gtatacctta tttttcattc gttgaccagt ctttttttaa tttctttttt
     5881 cgctggcgga gctttcggaa aaagtcagtc aaaatcatcc cacattcgtc ttctaaaatg
     5941 ccaccttcta catatgcttg gtggttaaac cgctcgtcct ccaaaagatt catcaaggta
     6001 ccggctgttc cgccttttgg atcatacgcg ccgaaataaa cttctgctac acgagacaaa
     6061 atcatcgcgc cgctgcacat aggacaaggc tctaaggtaa caaaaagtgc agcatcttcc
     6121 aagcgccagc ttcccacttt ttcacaggct tccttgattg caagcatttc tgcatgtgta
     6181 gtagcatctt gcgtggtttc tctaaggtta tatcctcttc caatcacttt tccatccaat
     6241 acaaccactg ctccaatcgg aacttcatga agcatttctg ctttctttgc ttctgccaat
     6301 gcaaggcgca tccatttttc tttttctact gcatcaagat ttgtatttac caattaccct
     6361 tccctgcctt tgttttttct tgttttactt tagcatatga taaaaaggct gtaactaact
     6421 tttttttcta tggtaaaata aatacaagag gtgaacaaga tgaaaatgga taaattactt
     6481 gctctttacc cacaagcaca gaaaaaaaac tctccagcat cagaagcgga tatcctttct
     6541 tttgcaattg atgatgcttt tatctggatc aaacaagatt cattatctca acaagaaacc
     6601 agccttttga aagcgttatt ccctgtaatc aacgatcaaa aaaaacaccc atggtatcac
     6661 tatcttttta aagatgcttc ttgtgttcaa gaaaaaagct ttcgtttgat ccagcttcac
     6721 gtcgaatcaa aaggagaatt cttaaaaaaa gaatggcaag atactatttt ggaattattt
     6781 cgctattctg ctgatttctt tttttataca gacacagatg cgctcttagt cgaacaaata
     6841 aaccgtgatt atcttgatgc tactgaaatc aacggtattt ttctatcttt agatgctgat
     6901 tttgatacga caacgtctgt attcgttggt tctttccatt cacctggcac cgacctcgtc
     6961 cctttgtttg ctgaagaaag aaaaattttt ttaacagagc aaaacaatct ttatactcac
     7021 agccgtactt tttctatgca agatgtggct ttacattact atacaaaaga tactgtaaaa
     7081 gatagtaagt tgatcaaaag ttacatagaa ctgatcaaac aaaatgaaat ggaagtgatc
     7141 attcaagaat tatggaatga attaggcaat gtcagctctg ctgcaaaaac actgtttctt
     7201 catcgtaata ctctaaaata tcgaatcgaa aaatttcaag aacagtctgg tttcaatcta
     7261 aaaaaagcca acgatttact gttttgctat cttttattgc ttcatgaaca taattaaaga
     7321 tagattaaag gggttgtgac aataattttg tcacaacccc ttatttcgaa tgaatagtgt
     7381 tcaaaagcta gtttctctta catcattttt tcaacaaaat gcattagccg gtcttttaca
     7441 tcttcggttg aatctttgat tgaatcacca taaacagaga ttttcttttt tcctgatttg
     7501 atttttgcta acattcccat caaagaatcc aagtcactat ctgaaagacc atctacgatt
     7561 tctagtaatt tttcatttcc atcaaatttg tcatagacga atttttttac tttcgtccga
     7621 gtagtcatat gatggatctt ttccattact tttcccgata caacgactgc tgtcgctaca
     7681 ctggcagcgg ctgctacgct taatccgatt gttacttttg ttgatgtttt cataagtatc
     7741 agctcctttt tatctatctc catcatagca tagacaaaaa aataaacgaa ctaatccact
     7801 cagtgaaatc tcaatccttt agggaaaaag ttctttactg ttccttttac ttgtttccca
     7861 aatccgacag gtatcttatc taatactagc aaaacccatc cttgattccc atcacgttga
     7921 aatgtttctc ctgctgtgta agattgccat tccttttgag tgatcggcaa gcaaggtata
     7981 ttctctatct tttttgtagc aagcgctaat gcatagctag gttcaaatcg attctttttg
     8041 aaatctccta aatgcaaacc tgtacgtacg acttttaaac catcgagaga aggggctaac
     8101 tcaggaactt cccaaagatg gtcgttgaac actaaaagtc ttccagtagc ttcataatga
     8161 aaatcatttg aaaactccgt ccagagtttt tcctgctctt ttgtcatttg cactttactt
     8221 ttttttcgtg tcttcttttc tttatgcatc tgattttgtc catgaaaagt cagctttgca
     8281 acaaaatgtc cttcaccctg atctttatga ggccatatac gtattgtttt ttccaaacca
     8341 gctacgcttc cccattcaga acgtcctgaa ctaacggatt gagtcaaagg aatctcctca
     8401 atcgttactg gataattttc tacaagccag ctaatgatct cttcattttc ttctggtgca
     8461 aacgtacaag tcgaataaat cagttgtcct ttatttttta gcatttttat tgctgaagac
     8521 aaaatctctt gctgacgttt ttgacagtat agcggagatt cttctgtcca ctcttttatt
     8581 gcatttggat cttttcggaa cattccttct cctgaacaag gagcatcaac cacgatccga
     8641 tcaaaaaagc ccgaaaaatg agggacaagt tctgcgggtg catgatttgt cactattgcg
     8701 ttcgaaactc cccatcgttc tatattttct gataaaatct tcgcacgttt cggaaagatc
     8761 tcattcgtca ctaataaccc tttccccttc atttgagcag ctaattgggt tgatttacca
     8821 ccgggagccg cacacaaatc caaaactttt tcaccgggtt ttgctgcagc agctgttcca
     8881 acgatcattg cacttggttc ttggctatat tcatacccag cttgatggag gaaagacttt
     8941 ccattgacag tgccaagaaa tccctcattg ctgtagggag cgggttgtaa ttcttcgcta
     9001 tggtaagttt ggaccatgtc caatcctgcc ggttttagtg gattccaccg aaatcctttt
     9061 ttcacgcttc cttgttcaag cgcagaaaaa aaatcggatg cttcttcgcc taaaagaaga
     9121 cgatattttt tgataaattg ctgtggtaac gttgtcgctt cctccttcat tccaatgttt
     9181 cgttagacat tttacaatct tttttacgaa aaataaaggt ggctgatcta ctttttcatg
     9241 ttgctgccta aggtgactgt tcctttaaga tattttccaa gtattcatcc gtcacctcgt
     9301 ttttatccca aacagcgttc atcatttcaa gaaaatcttg agaagaatca tctgagcgca
     9361 aacttcccaa tgcattaagc cataccaatt caagcgactt ttcttctttg atcttcgtgt
     9421 gacctttgac atatgagaaa aatggccgct taaatagcga atccatgtct aaatcagatt
     9481 ttgcaatgat ttcacgaata ctttcatgga tagaaaaaaa atctttcaat aagtagacaa
     9541 tgcttaaaac atcatcggaa ctgcattcag ctaagtagtt ttcaaaataa acagtgattt
     9601 cttctaatat cttctctttt aacccgctga ttgatttgaa atgtgtataa atcgggtatg
     9661 tagaacaacc gcagaactct gctaactctc ttgcagtaat tgacttcatt ccatattctt
     9721 ccacgtatac catacttttg aacagcaaat attctttact ataatttgtt actctcccca
     9781 tcattcttac ctccgtctct tttcatctac ccataaataa ataacataaa taattctaaa
     9841 a
//
LOCUS       DAIGOK010000103         9826 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.104, whole genome shotgun sequence.
ACCESSION   DAIGOK010000103 DAIGOK010000000
VERSION     DAIGOK010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.104"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(<1..658)
                     /locus_tag="NOA01_002112"
     CDS             complement(<1..658)
                     /locus_tag="NOA01_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCI0633294.1"
                     /translation="MTYKNIVFDLDDTLYDHQLPFKRSVEKCFPTIDIQQIDNIYKRF
                     RCWSDVAFPKYTKKLISIEQLRIFRCQKTMEEFGIDSISRTEALDFQADYEYELDQIT
                     MIPEIHQLLLALHKNRIPIGILTNGPVDLQSRKLQNIGAYQYFEKQNIIISQSTHGGA
                     AENSYEGNVKNLQSYQNVQLSKRAIEILKEEIELNHQHIRFNPDYKDNGWIFTSKSRH
                     K"
     gene            complement(663..1430)
                     /locus_tag="NOA01_002113"
     CDS             complement(663..1430)
                     /locus_tag="NOA01_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCI0633295.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            1920..2345
                     /gene="lacA"
                     /locus_tag="NOA01_002114"
     CDS             1920..2345
                     /gene="lacA"
                     /locus_tag="NOA01_002114"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HCI0633296.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            2362..2877
                     /gene="lacB"
                     /locus_tag="NOA01_002115"
     CDS             2362..2877
                     /gene="lacB"
                     /locus_tag="NOA01_002115"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HCI0633297.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            2888..3820
                     /gene="lacC"
                     /locus_tag="NOA01_002116"
     CDS             2888..3820
                     /gene="lacC"
                     /locus_tag="NOA01_002116"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HCI0633298.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            3823..4803
                     /gene="lacD"
                     /locus_tag="NOA01_002117"
     CDS             3823..4803
                     /gene="lacD"
                     /locus_tag="NOA01_002117"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCI0633299.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPAIKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            4831..5148
                     /locus_tag="NOA01_002118"
     CDS             4831..5148
                     /locus_tag="NOA01_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCI0633300.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            5148..6854
                     /locus_tag="NOA01_002119"
     CDS             5148..6854
                     /locus_tag="NOA01_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCI0633301.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            7001..8407
                     /gene="lacG"
                     /locus_tag="NOA01_002120"
     CDS             7001..8407
                     /gene="lacG"
                     /locus_tag="NOA01_002120"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HCI0633302.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            8530..8703
                     /locus_tag="NOA01_002121"
     CDS             8530..8703
                     /locus_tag="NOA01_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HCI0633303.1"
                     /translation="MKLPPLLKCGKWYVLTGKEWICHSDLELSAFEWEFLQSLDVEIR
                     ETIHFEVNYLPFQ"
     gene            8724..9623
                     /locus_tag="NOA01_002122"
     CDS             8724..9623
                     /locus_tag="NOA01_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HCI0633304.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNCPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDKL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         3094 a         1666 c         1976 g         3090 t
ORIGIN      
        1 gtttatgacg acttttggaa gtaaaaatcc atccattatc tttataatca ggattgaatc
       61 ttatatgttg gtgatttaac tcaatttcct ctttaagaat ttctatagcc cttttagata
      121 attggacatt ttggtaactt tgtaagtttt ttacattacc ttcatagcta ttttctgcag
      181 ctcctccgtg agtggactga ctaatgatga tattttgctt ttcaaaatat tgataggcac
      241 caatattttg gagttttcga ctctgtaagt caacagggcc attagttaga atacctattg
      301 ggatgcggtt tttatgtaga gccagcagta gttgatgtat ctcaggaatc atcgtaatct
      361 gatcgagttc atattcatag tcagcttgga agtctagagc ttctgtccta gaaatactat
      421 cgatgccaaa ttcttccata gtcttctgac agcgaaaaat tctaagctgc tcaatactga
      481 tgagtttttt cgtgtattta ggaaaagcaa catcagacca acatctgaaa cgtttgtaaa
      541 tgttatctat ttgttggata tcaatcgttg gaaaacattt ctcaacgcta cgtttaaagg
      601 ggagttgatg gtcataaaga gtgtcatcaa ggtcaaaaac aatattttta taagtcataa
      661 ttctagtccg cttttaaaat agtagtgtac tggctaaatt cctctaagtg ttgagggcta
      721 atctggttat ctgtaatgat ggtatcgagt tgatctagat tatagaagtt aaagaaatcg
      781 tatcgatcga atttagtact gtctactaat aagaattttt ctacagcatt gtttagggca
      841 agttgttgaa tcacaccttc cttgtcacta tatgtagcaa tagaattatg ggtaacagca
      901 tttgcactaa caaaagcttt ggcaaatcta attgctttta aatttgtcga agccattgaa
      961 cctacaaaag ctccagttat ttctctatat tcaccgccaa gaagcaataa atcaatggtt
     1021 tcgctatcat ttagaatcaa gaacacaggg agactatttg taatgacacg aattttataa
     1081 cctttacgac ctttcaattc taatgccagt tgttcaagtg tagttccggg tccaataaaa
     1141 atagtatcgc catctttgat taaagagcag gcttttttag caatgtcaat tttttctttt
     1201 gtatttagac ttttcttctc gatatgtgtc ttttcaagtg gctttttaga ggaaagcaat
     1261 tgtgcaccac catgagtacg tgttaaaagt ccatcagctt ctagagcatc aaggtctcta
     1321 cgggctgtca tatcggaaat atctagttca tctattattt cttttatggt tatggtccca
     1381 tcaatcttta acatatctaa aattttttct aatcgtcgtt ttttgttcat atgaagaccc
     1441 ttctttcata aagtaatttt tttccaaaga taattctctt ttaattgtat cataaaagac
     1501 aatattttca aggtaaaaca aacaatttca aacaaaaaca aatgttagat gatgaaataa
     1561 gaacagtgga ttgacgtata ttagcttagg tcagattttg tataagacga aaataaagta
     1621 ggacctctta atcagtaagt tatagaaagt aaaagacttt tgtaatactt gaatagatat
     1681 ttcacgtcca ttttgtgatg gattaaatga acaaaaatga acaataattt aacggtgtta
     1741 tctatttttt aaaaaaacaa ataaaaaaaa caaaaaataa acaaaaaaag tgttgctttt
     1801 tgtttgaatg cttgatatta tataaacaaa gaaatgatga aaacgttttt tcgaacattt
     1861 tgcaaaatgt tttctacttc tacgtagcat ttctttctaa aatttaggag gtggtcaaaa
     1921 tggctattgt tgttggtgca gatctcaaag gtacgagatt aaaagatgtt gtgaaaaatt
     1981 ttcttgtaga agaaggtttt gaggtaattg atgtgactaa ggacggacaa gattttgttg
     2041 atgttacctt ggcagttgcc tctgaagtaa ataaagatga gcaaaactta ggtattgtaa
     2101 ttgatgctta tggagctggt ccattcatgg tagcaactaa aattaaaggt atggttgcag
     2161 cagaagtttc agatgaacgc tcagcttata tgacacgtgg acataacaat tcacgaatga
     2221 ttacggttgg cgctgaaatc gtcggtgatg agcttgctaa aaacatcgct aaaggtttcg
     2281 tcaatggtaa atatgatggc ggacgtcatc aaatccgagt agatatgctt aacaaaatgt
     2341 gttaagaaaa ggagttaagg aatgagaatc gcaattggat gtgaccacat tgtcacagat
     2401 gaaaaaatgg cagtatcaga attcttgaaa tcaaaaggac atgaggtcct tgatttcggg
     2461 acatatgacc acgcacgtac tcactaccct atctatggta aaaaagtcgg tgaagcagta
     2521 gtaagtggtc aagcagactt gggagtatgt atctgtggta caggtgtcgg cattaacaat
     2581 gcagtcaata aagttccagg tgttcgctca gcgttggttc gtgatatgac atcagctata
     2641 tatgcaaaag aagagttaaa tgcgaatgtt atcggtttcg gtggaaagat tactggtggt
     2701 cttcttatga atgacatcat tgaagctttc attgaagctg agtacaaacc aacagaagaa
     2761 aataaaaaat tgattgcaaa aatcgaacac gttgaaacac ataatgcaca tcaagcagat
     2821 gaagatttct ttacagaatt ccttgaaaga tgggaccgtg gtgactacca cgattaaggt
     2881 ataacaaatg attctgacag tcacactaaa tccttcagta gacatctctt atcctctaga
     2941 aacattgaaa attgatacag tgaatagggt gaaagatgtt agtaagacag ctggtggaaa
     3001 aggcttaaat gttactagag tcttgtatga atctggtgat aaagttactg ctacaggttt
     3061 cttaggtggt aaaattggtg aatttattga aagcgaatta gaacaatctc ctgttagtcc
     3121 agctttttat aaaatctcag gaaacactag aaactgtatt gctatcttac acgagggtaa
     3181 ccaaacagaa attttggaac aaggaccaac aatttctcat gaagaagcag aaggctttct
     3241 tgaccactat agcaatctta ttaaacaatc agaagttgtg acaatttcag gaagtttacc
     3301 ttcaggactt cccaacgatt attatgaaaa acttatccaa cttgcttcgg atgaaggagt
     3361 agcagtcgtt ttggattgtt caggtgcacc tcttgaaaca gttctaaaat cgtcggctaa
     3421 acctactgcc atcaaaccta ataatgagga actttcacag ttgcttggaa aagaagtaac
     3481 aaaagatatt gaagaactca aagacgttct taaagagtca ctttttagtg gtatcgaatg
     3541 gatagtggtc tcattaggtc gaaacggtgc ctttgcaaaa catggtgatg ttttctataa
     3601 ggtagatatt cctgatatcc cagttgttaa tccagtcgga tcaggggact caacggtagc
     3661 tggtattgca tcagctttaa atagtaaaaa aagtgacgct gacttattaa aacatgcgat
     3721 gacattgggt atgttaaatg ctcaagaaac aatgacaggg catgtgaata tgactaatta
     3781 cgaaacacta aactcacaaa ttggagtaaa agaggtataa aaatggtact tacagaacag
     3841 aaacgcaaat ctttggaaaa aatttcagat aaaaacggtg ttatctcagc tttggcattt
     3901 gaccaacgtg gtgctttgaa acgtttgatg gcacagtatc aagatacaga accaactgta
     3961 gctcaaatgg aagaactaaa ggttttggtt gctgatgaac ttacaaaata cgcttcttca
     4021 atgttgcttg accctgaata tggacttcca gcaataaaag cattggataa agaagcaggt
     4081 cttcttcttg cctatgaaaa aacaggttat gatacatcaa gtacaaaacg tttgcctgac
     4141 tgcttggatg tttggtctgc aaaacgtatc aaagagcaag gcgctgatgc cgttaagttc
     4201 ttgctttact atgatgtaga cagctcagat gaacttaacc aacaaaaaca agcttatatt
     4261 gaacgtgttg gttctgaatg tgtcgctgaa gatattcctt tcttcttgga aatccttgct
     4321 tatgatgaag aaatctcaga tgcaggttct gttgaatatg caaaagtgaa acctcgcaaa
     4381 gtcattgaag cgatgaaagt tttctctgat ccacgcttta acattgatgt tcttaaagta
     4441 gaagtaccag ttaacgttaa atatgtcgaa ggttttgctg atggtgaagt ggtttacagt
     4501 aaagctgaag ctgctgactt ctttaaagca caagaagaag cgactaacct tccatacatc
     4561 tacctaagtg caggtgtatc tgctaaattg ttccaagaaa cattgcaatt tgctcacgac
     4621 tcaggtgcca agtttaatgg tgtgctttgt ggacgtgcta cttgggcagg atctgtagaa
     4681 ccttacatca aagaaggtga aaaagcagca cgtgaatggt tgcgtactac tggattcgaa
     4741 aatattgatg aacttaacaa agttcttgtt aaaacagcta gtccatggac tgataaagta
     4801 tagtaaaaac ataaaacgga ggatattgtt atgaacagag aagagatgac tctcttaggg
     4861 tttgaaattg ttgcttatgc tggagatgct cgctctaaac ttttagaagc gcttaaagcg
     4921 gctgaaaatg gtgatttcgc taaggcagat agtcttgtag tagaagcagg aagctgtatt
     4981 gcagaggctc acaattctca gacagctatg ttggctcgag aagcttctgg ggaggaactt
     5041 ccatacagcg ttaccatgat gcatggtcag gaccacttaa tgactactgt cttattaaaa
     5101 gatgtgattc atcacctcat cgaactttat aaaagaggag caaaataatg cataaactca
     5161 ttgaacttat tgagaaaggg aaaccgttct ttgagaaaat ctctcgaaac atctatcttc
     5221 gtgctattcg tgatggattt atcgctggta tgccagtcat ccttttctca tctatcttta
     5281 tccttattgc ctatgtccca aatgcttggg gattccactg gtcaaaagac attgagacct
     5341 tcttgatgac tccttatagc tattcgatgg gtatcctagc attctttgtt ggtggtacta
     5401 ccgctaaagc tttgacggac tcaatgaacc gtgacctacc tgcgaccaat cagattaact
     5461 tcttatctac gatgctagcg tctatggtag ggttcctttt gatggcagct gagcctgcaa
     5521 aagaaggagg cttcttgaca gccttcacgg gaacaaaagg tcttttgaca gcatttattg
     5581 cagcctttgt gacagttaat gtttacaagg tctgtgtgaa aaataatgtt actattcgta
     5641 tgcctgaaga ggttccacca aatatctctc aagtatttaa agacttgatt ccattcactg
     5701 tagcggttgt tctcctttat ggatttgaac tcattgttaa gggaactctt ggtgtaaccg
     5761 ttgcagaatc aatcggtacc cttcttgctc ctctcttctc agctgcagat ggttaccttg
     5821 gaattacact gatctttggt gcttatgcct tcttctggtt tgttggtatc cacggtcctt
     5881 caattgtcga accagcaatc gctgctatca cttatgccaa tatcgatgcc aacttgcatc
     5941 ttatccaagc tggacaacat gcagataaag ttatcacttc tggtactcaa atgtttattg
     6001 ttaccatggg tggaacagga gctacattga ttgttccatt cttgttcatg tggatttgta
     6061 aatcagaacg taaccgtgcc atcggacgtg cctcagttgt tccaacattc tttggggtta
     6121 atgagccaat cttatttggt gcaccaatcg tattgaatcc gattttcttt gtaccgttca
     6181 ttttcgctcc gattgtcaac gtttggatct ttaaattctt tgttgacaca ttgaacatga
     6241 actcattctc tgccaacctt ccatgggtta ctcctggtcc gttgggaatt gtactcggta
     6301 ctaacttcca agttctatca tttatcttag ccggactctt ggtagttgtt gatactatca
     6361 tttattaccc atttgttaag gtatacgatg aacaaattct tgaagaagaa cgttctggta
     6421 aaacaaacga tgctcttaaa gaaaaagtag ctgcaaactt caatactgct aaagcagatg
     6481 ccgttcttgg aaaagcaggc gttgctaaag aagatgtagc agcaaacaat aatatcacaa
     6541 aagaaactaa tgtccttgtt ctttgtgcag gtggaggtac aagtggcttg cttgccaatg
     6601 ccttgaacaa agcagcagca gaatacaatg ttccagtcaa agcggcagcg ggtagttatg
     6661 gtgctcaccg tgaaatgttg ccagagtttg acttggttat cttggctcca caggtcgctt
     6721 caaacttcga tgatatgaag gctgaaacag ataaattggg cattaaactt gcaaaaactg
     6781 aaggagctca atatatcaaa ttgacacgtg atggacaagg tgctcttgca tttgttcaac
     6841 aacagtttga ttaataaatt tgtaaaagtt atttgagacg agattattct ttcagttcct
     6901 tataatattg ttaaccatat cattgcattt catttcattt ccttggtttc cggaactcag
     6961 aatatcgtct caaatgcttt ttttgaaagg atttatagtt atgactaaaa cactccctaa
     7021 agattttatt tttggtggtg ctacagctgc ttatcaagca gaaggtgcga ctcacacgga
     7081 tggtaaaggc ccagttgcat gggataagta tcttgaagac aattattggt acacagcaga
     7141 gcctgcaagt gatttttacc ataaatatcc tgttgattta gaattagctg aaaaatatgg
     7201 cgttaatggt attcgtattt caattgcctg gtctcgtatt ttcccaactg gctatggcga
     7261 agtaaatgaa aaaggtgttg aattctacca taatcttttt gccgagtgcc acaaacgtca
     7321 tgttgagcca ttcgtgactt tgcaccactt tgacacacca gaagttcttc actcagatgg
     7381 agatttctta aaccgcgaaa atatagaaca ctttgtagat tatgccgctt tctgttttga
     7441 agagttccca gaagtaaact actggacaac tttcaatgaa attggcccaa ttggagatgg
     7501 tcaatacttg gttggtaaat tccctccagg cattcaatat gatcttgcca aagtcttcca
     7561 atcacaccac aatatgatgg tttctcatgc acgtgctgtg aaattgtata aagataaagg
     7621 ttataaaggc gaaattggtg tagtacatgc gttgccaacc aaataccctt atgatccaga
     7681 aaatccagca gatgttcgtg ccgctgagtt agaagatatc atccataaca aatttatctt
     7741 ggatgcaacc taccttggac actattcaga taaaacgatg gaaggggtca accacatcct
     7801 agctgaaaat ggtggagaac ttgatcttcg tgatgaagac ttccaagcac ttgaagcagc
     7861 taaagatttg aacgatttcc ttggtatcaa ctactacatg agtgattgga tgcaagcctt
     7921 tgatggtgag actgaaatca ttcacaatgg taagggtgaa aaaggaagtt ctaagtacca
     7981 gattaaaggt gttggtcgcc gaatcgcacc agattatgtg cctagaacgg attgggactg
     8041 gattatttat cctgaaggct tgtatgacca aatcatgcga gtgaaaaatg attatccaaa
     8101 ctacaagaag atttacatca ctgaaaacgg tcttggatac aaagatgagt ttgtagataa
     8161 taccgtttat gatgatggtc gtattgatta cgtgaagaaa cacttggaag ttttatcaga
     8221 cgcaattgcg gatggtgcaa atgttaaagg ttacttcatc tggtcactta tggatgtctt
     8281 ctcatggtca aacggatacg aaaaacgtta tggactattc tatgtagact ttgacacgca
     8341 agaacgctat cctaagaaat cagctcattg gtataagaaa gtagcagaaa ctcaagtgat
     8401 agagtaatat tacttgaaaa tgtaaaaaat tgttatccga tactttagaa aaagtacaac
     8461 acttatttag accaggcttt gaaaaggtat atattgttag tttcgaagat tgtcctatga
     8521 ttcctgagct tgaagctacc cccgttgcta aaatgtggta aatggtatgt tttaactgga
     8581 aaagagtgga tttgccattc tgatttagag ttatcagcat ttgaatggga atttttacaa
     8641 tcactcgatg tagaaatacg tgagactatt cacttcgagg taaattattt gcctttccaa
     8701 taataaaaag aaaggaatta gttgtgacta tcgaattaaa aaacgagtat cttactgttc
     8761 agtttaaaac acttggtggt caactgactt cgattaaaga caaggatggg atagagtatc
     8821 tctggcaagc tgatccaaat tattggaatg gacaggcgcc tattctattt cctatctgtg
     8881 gtagtttacg aaatgattgg gctatttata gacctcaaga aagacctttt tttacaggtc
     8941 ttattcgtag acatggtttt gttcgaaaag aagaatttat tctggaagaa gtcaatgata
     9001 atagcgtgac ctttagtatt aaaccaaatg ctgagatgct tgataattac ctatatcagt
     9061 ttgaactgag agttgtttat accttgaatg gtaaatcaat tagaaccgaa tttcaggtaa
     9121 cgaacttgga aatggaaaaa acaatgcctt actttattgg agcccatcca gcattcaatt
     9181 gtcctttagt agaaggtgaa aactatgagg attattatat tgaatttagt gaggttgagt
     9241 cttgctcgat accaaagagt tttcctgaga caggattatt agatttgcaa gatcggacac
     9301 cttttttgga aaatcaaaag attctcgatt tggactactc acttttttcc catgatgcca
     9361 ttacacttga caagctcaag tctcgtagcg taacgcttcg gtcaaggaag tctggtaaag
     9421 gtcttcgagt agattttgat gattttccga acttgattct gtggtctaca actaataaaa
     9481 gtccattcat tgctttggaa ccttggagtg gtctctccac ttcacttgaa gaaggtaatt
     9541 tcttagaaga taaaagacag gtaacgaaaa tttcacctca agaaacttct agaaaaatct
     9601 atgatattac tatccttaat taaaatttgt tattatttga gtcttcaata agaattgaat
     9661 actcttacct tgtgatgata gattggtctt ctccgtgcca gtcttacatc gcaaggtttt
     9721 taagtttttt gtgggtctat gtcaactgta gtgggtgacg aaaagctaac atctaaagac
     9781 agtctggtag taaaaaaata agattattta ctacttcttg atttaa
//
LOCUS       DAIGOK010000104         9737 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.105, whole genome shotgun sequence.
ACCESSION   DAIGOK010000104 DAIGOK010000000
VERSION     DAIGOK010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9737
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.105"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            59..3337
                     /locus_tag="NOA01_002123"
     CDS             59..3337
                     /locus_tag="NOA01_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HCI0633305.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3878..5785)
                     /locus_tag="NOA01_002124"
     CDS             complement(3878..5785)
                     /locus_tag="NOA01_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HCI0633306.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            5915..6790
                     /locus_tag="NOA01_002125"
     CDS             5915..6790
                     /locus_tag="NOA01_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HCI0633307.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6870..7844
                     /locus_tag="NOA01_002126"
     CDS             6870..7844
                     /locus_tag="NOA01_002126"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCI0633308.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8024..9169
                     /locus_tag="NOA01_002127"
     CDS             8024..9169
                     /locus_tag="NOA01_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCI0633309.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9306..9650
                     /locus_tag="NOA01_002128"
     CDS             9306..9650
                     /locus_tag="NOA01_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HCI0633310.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
BASE COUNT         3173 a         1800 c         2060 g         2704 t
ORIGIN      
        1 aataaattag gtaatacttt aagtaaacgc tttctaattt gttacgggag gaatacactt
       61 gttaaaaaaa cgtataaaat acagtgcgct aatgatgaat ctccttatgc tcagtacccc
      121 ggttttaatt tctgtcgttg aagttgctgc taacgaacaa cagtctgtga acgaagaaaa
      181 tgcacacgtg aacattgaag ggatgtcaac aatggaggaa ataccaagaa ctagtgaaga
      241 tatggatacg gaagaaggaa atgatcctat gatgaaagaa tcagaaaatg aaaagacaaa
      301 aaagcaatct gaagaagcaa atgaagagtg gaaaggggtt gttttcggag aaagcacaag
      361 tgctagtagg tatgccattg aaccattaga agacggcgtg cggctctctt ccacgaataa
      421 cggcggaaaa ttccagtcaa gcggatcaga tggaatgacc tattattatc aagcaatccc
      481 caaagacatc aactttagga tgcgggcgac gatcgaaata gaatcttgga cctatacgaa
      541 tgcccaagaa ggatttgctt taatggtaag agacgcagta ccaaaaaatc atttgtttgg
      601 gcaggcattt tattcaaatt catttgccat actaggcagt cggatcgaat atgtctggga
      661 ctcggataga caagaagtgg tcaacacgag cggcagcaaa tatacgatgc gacttggatt
      721 ggggacacgg gcaatcacag gaatatcatc tgaagatccg caagctgcgc cagctcctgg
      781 gagtgtctca gtggaaacaa cacctttaga gacttcagca gggttcttgg aaaaagaaac
      841 aggttcttat aatatattcg gaaacggtca tgggaatctg tttccggcca cgaactcgat
      901 tactaaactg gacgtagaga tgaaaaaaac gaatactggt ttagaggcat attattacga
      961 tcctgaaaca ggagaagaaa tggcttctga catcatgtat gactgggaaa aattatacgc
     1021 ttcagatgaa tcggttattt atgcaggatt tgctgcagca aggaatatga cgatcaaggt
     1081 agaaggaatc gaagttgctt actcagatcc tgcgacagat cctcctgggc aagaaagacc
     1141 tactcgcttg attgatccga tttacactgt gacctctagt aatcattcag gaaaccaaga
     1201 tcatactatg tcctttagag caaatgctga cggattattg acaattaaaa ataaagcaac
     1261 aggagaggtg cttaagaacg caaaaaactt ggctatcact gcgaatcggg aatttgatta
     1321 tcagacaaaa ctgatggaag gaacgaacga atttactttc agttttacgc cagataaaaa
     1381 ctatccgcca gaagaatacg tagagatgac tagttatgaa acaaaagaga ttcttcatac
     1441 agtggactac cgtaaaccta aatattcgac agtttatgta acaccagaag ggtcagatgc
     1501 aggaaacgga agcagacaaa atccattatc tctgacacaa gcattgagat acgcatatgc
     1561 tgggcagaca atcgtcatgg cgcctggaac ttattccttc gaaagaggat tgacgattcc
     1621 aaaaggcgtt aacgggacaa gagaaaaccc gattcagttg attgcagaga aaaatccaga
     1681 aaacaagcgt cctgtacttg attttaaagg aactggaaat gggatgacac tgttcagtca
     1741 ctactggggt ttacgagggt ttgacattac aaatagtgca gctatgcaaa aaggattaca
     1801 gatttctgga aatcataatt tgatcgaaga gattcatgct tatcggaatg gaaatacagg
     1861 aatccaaatc agcggtagct caaatgatcc tttcgaagca tggcccgcgc ataacttagt
     1921 caagaactgt acatcttttg aaaatgccga tccaggattt gaagatgccg atggatttgc
     1981 ggccaaactg acaataggag aaggaaatgt atttgacggt tgtatcgcgt atcataatgc
     2041 agatgacggt tgggaccttt ttgctaaaaa tgaaacaggt tcgatcggaa aagtaatcat
     2101 caaaaactca gttgcttatc gaaacggctg ggtaccagga attgagggag aaggtaacgg
     2161 taatggattc aaaatgggcg gttcttcttt aacgggccct catgaattga tcaatagttt
     2221 gtctttcgaa aatcttgcga aaggaatcga ttccaatagc ggaacagata ttatcgtcaa
     2281 tagcagtaca agttttaata acggttcata taatgtggct ctttatacct cttcagcagg
     2341 aaacacagac taccatgctt ctggtatctt atctttccga acagagaatc ttgagatgag
     2401 agagcagatc cgcccattga atcaaaacga agatcaaatc taccactcgt tgaactacta
     2461 ttggaatgaa cgagagaaac aatcagaaaa tactcttgga cataccatat caaaagattg
     2521 gatcgagtct ctaaatacat gttcgaaaga aggtcagcag cctgttacgc gctatgaaaa
     2581 tggaagtatc catgttcatg gactgatgca gattaaaccg gggaatcgtc aaacagaaaa
     2641 ggaaagagtg gatggtcttc cattatctgt aggtgctatt tttgacggag aaacctcacc
     2701 gtcttcggaa tatccagagt atgcaatcgt acaagaacca aatcagacaa aaccaaatga
     2761 acaaaataat ccgattatta tacaagaagc aaatggtgtg gcggagaatg cagaaggaaa
     2821 tatcattgaa ctgaagcctt ttacagtttt tgtgtcaact ttcacaccaa atcaaggaac
     2881 gcctgtcttt gaagatatgc gttatttccc agaaaagatc caaatcgttg aaacacagtc
     2941 aagtgctcaa ttttctaatc ataccgctat tttttcagca ccttcaaaag gagaatatac
     3001 gctagaagtc acttatcgtt cggaaatatt tgatggggaa aaatggtctg ctcatacaga
     3061 atctgttacc cagcaaaaag cgatcactgt gcgagaaact gccgatgtgc ccccgggaaa
     3121 tgacaaacca gaaagagaaa atccaggaaa tgaatcacca aatggaaaac caaatggaaa
     3181 accttctcat ccggatagga cagcaaaaga aagtgaaaaa aatggcactc aaggactccc
     3241 aaaaacggga gaatttaata acccgttgct agcattagct ggaggaattt tgttaatcgg
     3301 tgtagttgtt tatgtcatga aacagcgaaa aaaataagtg gaatttctcc ttttatgatg
     3361 taaaacaatt gactgaggaa agtaaaaata gaaggaaatg agaccaatag atgtctcact
     3421 tccttctatc tttttataga tgaggttctc cttgctgctt ttgattggct gttcttatta
     3481 cttgatgcag gacagctttt tcacaatctc tccactaaac ggtgttcaat cagctgaccc
     3541 tcggtattag ctcaacaaac ggcaaaatat gaggaactat ttttccgttt ttttctctaa
     3601 tactcaggtc aggacgctga tttcacaacc tctcgataaa cggtgttcaa aagccagttt
     3661 ctcggtagta agtcaaattt atgcaaaaat gtggaaagcc attttcacaa aatttttctt
     3721 actacttgaa actaagcagc tttttcacaa cctctaatac atggtattcc agaagtaact
     3781 tcttcgaaaa ataagccgaa atgtcacaaa aatttgagaa gcgattttcc caaattcctt
     3841 cttatttctt gaagctgacc acttctggca caacctctta ccagattcgc actctttttt
     3901 ctggcgctag atacatagcg tcttgttccg tgataccaaa tgtttcatag aattcgtcaa
     3961 ggttttttgg ttgcacgttt gctcggagtt tagcaggggc atggacatcg atttgcagta
     4021 aaagttgttg gtattctttt tttgcttttg tacgccaaat cgttgcccaa ttgacaaaga
     4081 attcctcgat agaaagatct gattccgttt ttgctgcttc caatgcacag cttagaccgc
     4141 ctgcatcagc aatattttcc gaaacggtta attttccgtt tacttttccg tcagcaaaag
     4201 gcagaccatc gaattcttcg atcattgctt gtgctttgtt ttgaaattgt gtaagatctt
     4261 cttctgtcca ccagttgttc aagttgccat attcgtcaaa taaagcgccg ttattatcaa
     4321 atgcatggga aatctcatga gcgataacag ctccgattcc accataatta gcagagcttg
     4381 attgttctag actgtaaaat ggtgcttgaa ggatcgctgc cggaaagacg atcacattcc
     4441 gaaatggatg ataataagca ttgaccgtgt cagcactcat ttcccattct gaacgatcca
     4501 ctggtttgtt ccatttgctg aaacgatctt ttaatgtgag tttgctgaaa acaagagtat
     4561 ttgaaagcaa agtaccaccc tgatctgcag gtactgtttt gaactggtcg aataatgctg
     4621 ggatcttatc ggggtagcct acttggatac ctaagttatt taattttatg acggcttttt
     4681 cttttgttgc atccccaagc cagtcatttt cttctagacg ttttttataa acggcgatca
     4741 tttttttcac catttgttct acgtcttttt tagcagcttc gccaaaatat ttttttccgt
     4801 agtaatcgcc aactacttga tcgaattgtc cagaggcaag gtaatacgct gcttttttct
     4861 gaggcattgc ttcgtctgtt ccagacaata cgcgagaata gattccgctg acttggcgga
     4921 attcttctga tagataacca ctaagagaac gtactgtttt aatgatcatc cagcttttga
     4981 ataacggaaa atgagcttct gtcaggatgt cattcaaatg atcgaagtac actggatcag
     5041 ttacgatcac tttatctgga gttgtcccaa tcaaacccgt gacaagtttt ttcaagtcca
     5101 gtttatctgt gtatttaata aatgtatcaa aagattgcgg attatacatt ttgctgtaat
     5161 ctgcgttttc ttctgcactt tttacatgag gagcaatcaa tttatcaaat tgcactgctt
     5221 gttccgtgat tgcttctgcc tgactttttt ctttgccggc aagctctaat aactgaacag
     5281 ccatatcaaa gaaaacgcct attagttgtg caccgttggg atggtctgat gcataatacg
     5341 tcttatctgg aaggaataaa gaaggcggat aagcaaagaa ggcgttcgtt tgcgcattct
     5401 tcatatctgc atctacatcc agattaaatg gcaatggtaa tgtatccaac agccattctg
     5461 gaaattgctt attcaattcg tcgaaagact gcaatgattc aactttttcg attataggtt
     5521 gaagcggttg agcatgcaac tcatttcttc ggtcataatc gtttgccagt tgataatatg
     5581 caaggaaatg tttcattttt tctgaagaca ctttttctgg atgggcaagc agatcgtctg
     5641 tttctcccat caaaagttgg tcgatcccgt caactaaatc ttggaaaccg cctgtagctg
     5701 gtttatctgc agggattttt gctgtttttt gccattcttc attgactgct tcatagaaat
     5761 cttgtttcaa tcgttctttg ttcattcatc gatcgctcct tttttgtttc gtgtgcccat
     5821 tatcttacaa aatgaaggaa ttaacaaata gaacataaaa aattgaaaat tcgaacaatc
     5881 aaactgattt gtttttttca atccttcact tataatgaaa aaaaggaggc gatcccaact
     5941 gaatcaagta gtggataaac ctttatattt caaagtagat aaaaaaatta aaaaattagc
     6001 cagcactcag cagctgcagt caagaaaaag taaattgctt tttttagctc tggtttttga
     6061 agatcaaagt tatgtgatta tcgatcaatc aggacatccg gtagaatatt ctccagcaaa
     6121 atatacttat caagagggtc tttcttgtaa aaaatggaag ctgatcaatg aagagccaat
     6181 cgagttatct agatggatca acagaaaaga agatattccc gttttgatcg aagaaaaaag
     6241 gtcaggaaag gaactcgcaa attgctgggt gggattgcca gaagaacgtt ttttgaggta
     6301 taagaaatgg gctaccccct caggctatct ttgtggtact tacgcggcag cagttctcct
     6361 tgcctattat caagactatc gtaaggggtg gatgcttcca aatgagattc gcaagaaaaa
     6421 tacggctgat tcgttggttt tgacgaaggc attgcgtagc cagatccaac cactaggact
     6481 tcctacgatt ccttttcagg ttagtacagg gatttctagt tttttaaaga agaatggcaa
     6541 tcatgagcgt gcacgagcaa ctcttctagg ttcatggcag cgagctacaa aacgtatacg
     6601 tgaaggcaaa cctgtgatga ttggtatttt gaaagtactt ggcagtacat atggcaatca
     6661 ttgggtgaca gcgtacgctt attttgaaac tgaaacagga gaacgttatt acaaagtaca
     6721 cgacaactgg ggcgattatc ataaagtcat tcctgccagt tggagtaacg gaacagtttc
     6781 acttccttaa gaatttgctg aaattaacag aaaatcttgg aaaaatatgt taaactatcg
     6841 aagaatagag aagttttggg gaggactaca tgagtaataa gtatcaggat gacacgttga
     6901 agatttttag tttgaatggc aaccgtccat tagctgaaaa aatcgcgaaa gtgttcggaa
     6961 cagaattagg taaaagcgtc gtgaagcaat ttagtgatgg cgagatttcc atcaatatcg
     7021 aagaaagtat tcgaggggat catgtgtata tcgttcaatc aacgaatgca cctgtcaatg
     7081 attactatat ggaactactg atcatgatcg atgcaatgaa gcgagcaagt gcgaaaacga
     7141 tcaatgtcgt attgccttac tatgggtatg ctaggcaaga ccgcacggcg aaacctcatg
     7201 aaccgatcac tgcgaaattg atcgctaact tgatcgaaga agcaggggca actcgagtac
     7261 tgactttaga tctgcacacg gttcaagtac aaggattctt tgatattcct gtagataatt
     7321 tatttacgat gccgttgttt gctcattatt accgtcaatt aggtctgacg ggagatgaca
     7381 tcgtagttgt ttcgccaaaa aacagcggtg ttcagcgtgc acgtagtctt tcagaatatt
     7441 tgaacagcac attagccatc gtagatcatg cagatgatgc ttcaactgag ggtggttatg
     7501 tgatcggtga tgttcaagga aagacatgta tcatggtaga tgatatcttg aacacaggag
     7561 caacgcttgc acgagcagca aatgtattaa aagaaaacgg cgcaaaagaa gtatatgcct
     7621 gtgcgtcaca tggattattg tccgatcatg cgaaaagtat tttagacaat gcaccaatca
     7681 aagatatttg tatcactgat tctgttttca cagaagaaaa tcgtcatcca gaaaacttaa
     7741 caatcgttac atgttcagaa ttaatgggtg aagcggtcaa acgcatccat gaaaatacac
     7801 cgatgagtcc gttattccgt ctggaagaaa aagaattcga ataaacaaaa aaaagaggga
     7861 aagaagttgt gacagaagtg agcagcttca aggagctgcg acaagacttt tgtcacagct
     7921 ccttttttct atgcccatga aacgtcctct agacaaaaat gagctcttct ttggtttgtt
     7981 cgctatttta tgctagaatg tgcctagcaa taatttggaa gggatgaatg aagtgaaaac
     8041 tatttatctg gatcatgcgg caactacccc catgcatcca caagtaattg aagcgatgac
     8101 ggcaatcatg caagacacgt ttggaaatcc ttcaagcata catggatttg gtcgccacgc
     8161 gcatgaaaaa ttggaaacag caagacaaat cattgcagac agtctgcaag caaaacctca
     8221 cgagatcatc tttaacagtg ggggaacaga aggggataat acagctatct tagaaacagc
     8281 gttctcgcgt caaaaagaag gcagacatct aatcactaca gctatcgaac atcctgctgt
     8341 tcttaatacc atgaaatatt tagagacaaa ggggtttgac gttacatatc tgcctgtaga
     8401 tgaaaaaggg aatctgtcga tcgaagaggt caaaaaagcg ttacgagaag atacgatctt
     8461 ggtctctgtg atgtacggga acaatgaaat cggaaatttg atgccaatca aagaaatcgg
     8521 tgaactttta gcggatcatc cagcttattt ccatacagat gcggtacaag cgtacggaaa
     8581 ccaaacgatc catccagaag aattaggaat cgatttatta agtatctctg ctcataaaat
     8641 caatgggcca aaaggcgtcg gtttcttata taaacgagac ggagtaaata tccctgcatt
     8701 gcttttaggc ggggagcagg aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat
     8761 ctggggtatg gcaaaagcag ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa
     8821 caaatataaa ggattccagc aattgattct tgatacatta gaagcaaacg aaatccctta
     8881 tcaagtaaat ggtgatttaa cgaataaact ccctcatgtg ttgaatcttc gcttaattgg
     8941 cgaagcaaat gatttgctac tgatgaagct tgatcttaat ggggtcgcta tttctactgg
     9001 ttcagcttgt acagcaggaa atatcgagcc ttcacacgta ttagaagcaa tgtatggaga
     9061 agatacaccg atactaaaag aatccatccg tatcagcttt ggtttgggaa ataccgaaga
     9121 cgacatccag tatttccttg atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc
     9181 attcttatat gaacagatgt ctgagagaac gccttcattt tttagggaac ttaactgaaa
     9241 aacctagtaa aaatcaggat aaacttttat aataaagaga aactaatttt atcgaggtga
     9301 aaatcatggc atttgaaaca acagcaacgg tactgggcgc accaattagc tatcgtttgc
     9361 atcctgatgc aaaaagatat acattgcgcg ataatggatt tacagaaaca aacggaggca
     9421 attatcaatt gatccgacca ttagatgcca cgccgcaaag caaagaagga ttcaaattaa
     9481 aaatcactgt atcaaaagat attcaaacat tgaaaatgtc gatcactaca gcaaatggat
     9541 tgaaagcagt caatattttc aaagatccaa aacagaaaat gagccaagat aaattttatt
     9601 ttctgatgga tgggatgatt agccgtgggt tattcgaaaa agtcgaatga tctgtggata
     9661 taaaaagaca actagttttg aaacggccta ttgttagatg actaactttg taaggacgac
     9721 gcaaattagt tgtcttt
//
LOCUS       DAIGOK010000105         8950 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.106, whole genome shotgun sequence.
ACCESSION   DAIGOK010000105 DAIGOK010000000
VERSION     DAIGOK010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.106"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            69..887
                     /gene="purR"
                     /locus_tag="NOA01_002129"
     CDS             69..887
                     /gene="purR"
                     /locus_tag="NOA01_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HCI0633311.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1390..2763
                     /gene="glmU"
                     /locus_tag="NOA01_002130"
     CDS             1390..2763
                     /gene="glmU"
                     /locus_tag="NOA01_002130"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCI0633312.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2857..3828
                     /locus_tag="NOA01_002131"
     CDS             2857..3828
                     /locus_tag="NOA01_002131"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCI0633313.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3834..3936
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4002..4571
                     /gene="thiT"
                     /locus_tag="NOA01_002132"
     CDS             4002..4571
                     /gene="thiT"
                     /locus_tag="NOA01_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HCI0633314.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4652..5425)
                     /locus_tag="NOA01_002133"
     CDS             complement(4652..5425)
                     /locus_tag="NOA01_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633315.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            5692..7392
                     /locus_tag="NOA01_002134"
     CDS             5692..7392
                     /locus_tag="NOA01_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HCI0633316.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            7362..7580
                     /locus_tag="NOA01_002135"
     CDS             7362..7580
                     /locus_tag="NOA01_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HCI0633317.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            7600..8490
                     /locus_tag="NOA01_002136"
     CDS             7600..8490
                     /locus_tag="NOA01_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCI0633318.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            8508..8792
                     /locus_tag="NOA01_002137"
     CDS             8508..8792
                     /locus_tag="NOA01_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633319.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
BASE COUNT         3018 a         1532 c         1887 g         2513 t
ORIGIN      
        1 gctcgaattt ttgaagaaaa acttatataa tgtaaaacga taaaatttct taaaaggagt
       61 tggaataggt gaaagtacgt cgcagtgaac gtctagttga catgacacag tatttattga
      121 atcacccgca tgaattgatt tccttaacaa gctttgccga acgttatgaa tctgctaaat
      181 cttctatcag tgaagatttg ggaatcatca agaaaacatt taaagaacga gggtatggaa
      241 ccttggaaac aatcccgggt gctgcaggtg gtgtattgtt catccctgaa atttcatatg
      301 aagaggcgaa aaaatatatt gaatctctag cagaacgtct atcagaacaa gaccgtctgc
      361 ttccaggagg atatgtttat ctctctgatt tgttaggaga acctgaactg ctgcgtcaag
      421 ttggaagaat cattgcttct aaataccttg gtgaaaaaat cgatgctgta atgactgtag
      481 ccacaaaagg cgtacctatt gcccaagctg tgtcctatta tctgaatgcg ccattcgtta
      541 ttgtacgccg ggactcaaaa ataacagaag gatcaacagt aagtgtcaac tatgtgtctg
      601 gatcttctga aagaattgaa aagatggaac tgtctaagag aagtttgaaa cgtggttcac
      661 gtgtccttgt agtggatgat ttcatgaaag gcggcggaac agtcaacgga atgaagagct
      721 tgattgaaga attcgaatca gaacttgttg gcatcactgt atttgccgaa tcaaaattca
      781 acggtcatcg cgccatcgat gattatacgt cactgcttta tgtcaaagat gtagatactc
      841 aaacgaaaaa tattacggtt gtgccaggaa attatttttc agaataagag gttgtgaaaa
      901 agctgcttag tttcgagtag taagaaaaat tttgtgaaaa tggctttcca cattttttca
      961 taaatttggc ttaataccga gaaactggct tttgaacacc gtttatctag aggttgtgaa
     1021 atcagcgtcc tgtcctgagt attagctcaa taaacggaaa aattgctcct catattttgc
     1081 cgtttattga gctaataccg aaggtcagct gattgaaaaa cgtttaatga agaggttgta
     1141 agcctaccgt ttaactctaa gtcacaagaa acgatttcaa aaaacagctt ttcatgtttt
     1201 atgagtagtg tgacttgtga ccgaggagtt ggcaggagag caccggttac caaatagata
     1261 ggcccgttat ggtctatcta tttttttatc cagaaaacgc tgggataaaa gaaaatcact
     1321 tgttttgttg aaaaaatggg tatactggga taaactgaaa tcagcaaact atatttagga
     1381 gtgacgcagt tggacgcacg ttatgcaatt attttggcgg cagggaaagg tacccgcatg
     1441 aagtcaaaat tatataaagt tttacatccc gtttctggta aacctatggt agaacatatc
     1501 attaatcggg tcagtgaaac gaaaccagat gaagtgatta cgatcgttgg acacggagca
     1561 gaacaagtta aagcacaact aggagaacgc agtaaatatg cgttacaagc agaacaattg
     1621 ggaacaggac atgctgtttt gcaagctgct tctttcttgg aaggaaaaaa aggaacaacg
     1681 ttagttatca gtggcgatac accattattg actactgaaa cgctgaacaa tctttttgaa
     1741 tatcaccaag gaaaaaatgc aagtgcaacg atcttgacgg cacaagcaga aaacccaaca
     1801 ggatatggac gaatcatccg tgaccatatt gggatcgtag aaaaaatcgt ggaacaaaaa
     1861 gatgcaacac cagaagaagc tcttgtacaa gaaatcaata caggaactta ctgttttgac
     1921 aacgaagctt tgttcgatgc gttaagcaaa gttggaacaa acaatgctca aggtgaatac
     1981 tatctaacag atatcatcga aatcttaaaa gaagaaggcc atacagttgc tgcttatcaa
     2041 acggatgatt ttgaagaatc aatgggagtc aatgatcgga tcgcgttagc taaagcaaat
     2101 gagatcatga gaaagcgaat taaccagatg catatggtca atggcgtaag ctttgtcgat
     2161 tcagcaacga catacatcga tgcaggcgta gaaatcggtc cagacacatt gatcgaagca
     2221 ggtgtacaaa tccaaggaaa tacagtcatt ggttctgatt gtgtcatcgg gtcacattca
     2281 aaaatcgtag acagtaggat tgaggatcac gtcgtcattg aaaactcagt cattgaaagc
     2341 agccatgtca agaaacatgc agatgtggga ccttatgcac atttacgacc aaaagctgaa
     2401 attggcgaaa acgtccatat cggaaacttt gtggaagtga aaaatgctca aatcggcaaa
     2461 ggaactaaag tcggtcactt gacttatgtt ggtgatgcta cgctgggaga agaaatcaat
     2521 gtcggatgtg gtgttgtctt cgtcaactat gatggaaaga acaaacatca tacgactgtc
     2581 ggcgaccaca gctttattgg ttctagcacg aatatcatcg gacctgtcga ggtagctaaa
     2641 aacagttcga ttgctgccgg gtcaacaatt acagacaata ttccagaata tgcgctggca
     2701 attgcgagag caagacaagt caataaagaa ggatatgcaa aaaaactccc ttatctaaac
     2761 taagactttt gcaaaatcac ttgatttatt taaaaattat gactactatt tattaggtag
     2821 aattctagag ttgtgaaaaa gaaagtggag gtccccatgt caaaacatta ctttgaccca
     2881 agattaaaaa tttttgcgtt aaattccaat cgcccattag cagagaaaat tgcagcagca
     2941 gtcggcgtcg agttaggaaa atcatctgtt actcaattta gtgatggaga aattcaagta
     3001 aacattgaag aaagtattcg tggatcgcat gtctatgtta ttcaatcaac gagcagccca
     3061 gtcaatgata acttgatgga gttgctgatc atgattgacg cactgaaacg agcaagtgca
     3121 aaaacaatca acgtagtcat gccttattat ggctatgcgc gtcaagaccg taaagcacgt
     3181 gcacgggaac caatcacagc gaaattagta gcaaacatga ttcaaaaagc tggtgcaaca
     3241 agaatgctga ctttggacct tcatgcagtt caaatccaag gtttctttga tattccagtc
     3301 gatcatttga tgggtgcgcc acttattgcc aattatttct tagaaaaagg aatcaaaggc
     3361 gatgatgttg tcgttgtttc accagaccat ggtggtgtca cacgggcacg taaattagcg
     3421 gaatttttga aatctccgat tgcgatcata gacaaacgcc gtccaaaagc aaatgttgca
     3481 gaagtaatga atatcattgg acaagtcaaa ggcaaaactt gtgtactgat cgatgatatg
     3541 atcgatacag caggtacgat cacattggct gccaatgctt tgaaagaagc tggtgcaact
     3601 agtgtgtatg cttcatgtac acatccagta ttatccggcc ctgctttaca aagaatagaa
     3661 gattcagcta tcgagcgtct agttgttaca gactcgatct atcttccaga agatcgtaaa
     3721 atcgacaaaa tcgatgaagt aagtgttggc ggactaatgg gagatgcaat caaacgtatc
     3781 catgaaaaca aaccagttag tccattgttt gaaacaaaaa ataaataata aataaactca
     3841 caaaggggag tccaattggg ctgagattga atctatttct aaacccttcg tacctgtatc
     3901 ggttatgcga gcgtaggaat tgtgaataaa cagctgtttg actattttcg aaagctgctt
     3961 cctcctttgt gactttttta ttcaaaggag gaagtttttt tatgggaaaa aatagagttt
     4021 gggtagaagg aacgattgtt gcagcgttag cgatggtgtt gtcgttgatt ccgatccaga
     4081 tcggcagcag tttttctatt tcattagggc agattccatt gactcttttt gcgttaagaa
     4141 gagggacaaa ggcagggatg ctggcaggtt tcatttgggg catcctacat tttccgttgg
     4201 gacaagttta ttatttaagt gtcgttcaag tgttgattga gtacccgatt gcctatactt
     4261 ttgcaggcgc agcagggctc atggcttcaa atgtccaaaa aggctatgta gacgataata
     4321 cgaaacaaat tagaaaaagc atcgtgtttg gtgcattgat tggcgcactt tccagatatt
     4381 tttggcattt tattgcagga gtggttttct ggggagcgta tgcactatgg gggatgaatc
     4441 cttggctctt ttctttcgta atgaatggtg ccagcgggtt gagtacagct attgtaacga
     4501 tcatcgtttt attagggatc caacaaacga ctccttcgat ttttgtgcca gataaacgac
     4561 aggagctgta aataaaaaag aagcaagaaa cttgagacag aatcatgtcc tagtttcttg
     4621 tttccttttt attttttgat aagaaataga tctacttcaa tggcagataa gaaagatcgg
     4681 catcttctac caattgccag tgtcgatcgg tgacagagta gattaaagta gcgatgattt
     4741 cctcttctcg ataccgggtg ccttttaatt tccaaatata atcttcaatc acatagattg
     4801 ggatacgata tttaccatcg atcaaaccaa cttgatcaaa agaaaaaatg atcccttcct
     4861 gcatcaaatt acgcatttct ttcaataatt ccacactgat tttttcaaca ggtgtttctt
     4921 ttttgaaaaa agattttttt acattgatct tatctgacaa gatcgaactt aatttactag
     4981 caaaaatcct ataaaattca tctaaatagc gataataaat ccgattcagc tgatcattaa
     5041 gatcgaagta tacaaaatta ttctgtagtt tataaaaaaa cggcgaatga agatgtgttt
     5101 tcatatgacc aaaatataat aattctgaaa tttctaaagg tgtcaattct ttcagcatcg
     5161 ttaaatcagt aaagtcgatc cacttcaatt catcgaatcc acgcctgtta cgagaagagg
     5221 aaaagaaacg ctgaatattt tctgtaccac ggattacttt cagccctgta tgggattcat
     5281 attctccagc agaagcagat ggatcaagca agagcaagtt ttgaggaaaa tgcacgatac
     5341 tgtttgcaaa gtcagtagca gtcagccctt ttgttaaaac agcattactt gtggtatcaa
     5401 tatgtacata aactgactca gtcatcatgt tcctcctctt atttcctctt aatctctttt
     5461 attttacaac atttgcatta aaaaagcttg tttcttttat tttaaaattc cattacaaaa
     5521 tcacgttttc attgaaaaca ctctcatttt caattaattt ttgtgatttt tgtatttttt
     5581 tatttaaagg aggttttatg aattgttttc ttatggaatc tacaaagtcg aatacgagac
     5641 tcatgacctt tttatgacgt actggtatac ttgaagaaaa aggagagcgc gatggagata
     5701 aaaaaagttt acttttcaca gaaaatgtcg ctgtattcgc ctatccagca agcaaaacaa
     5761 ccccttccat tttttcaaag tcatcaagaa agtagagacg ctttttggcg ttctgcaaaa
     5821 aagcaatgtg cttttaaagc agtatcgaaa aaatataccg tctattttac tattgctaaa
     5881 agtagaggag atgttatttt taataatctg ttgattgaag gtgaaacttt tgaatggaaa
     5941 aaattttttc ggtatatgga agcttgcgct cgttttttta ttaaagaaaa ctgttgtttt
     6001 ttatttcaat ctcctctatc agaagaatgg ttgcgtattt ttcataaaaa cggctatcaa
     6061 ggaaccacac aattaaataa aaagctggtc tatcatacag cacttgtttt aggtggagga
     6121 ggagcgcacg gtgcttatca gattggtgtg tggcaggcgc tgaaggaaca tgatatcaac
     6181 tttgagatta tcacaggtac ctcggtaggt gcgttgaacg gcgcattgat tttacaagga
     6241 gatatgaaaa aagctgtgaa tttatggaaa aagctgtcga caagacaagt gcttgcgtta
     6301 ccagaaatgg ctgcagttga agaccttcgt gaacgcttct atcgagaaag acgccaaatg
     6361 acgaaaacag cactaattga aggcggggtt tcttctgctc cattggaaaa gatggtcaaa
     6421 gacaatttgg atttgtcctt actcaaacat aatcccaaaa tacgtttata tacggtgtct
     6481 actaaactgc cagaactagc ggaagtagtg actgatatcc aacagacaaa aacagaagaa
     6541 ataccagatt ggatactcgc ctctgcttca ttttatcccg ctatggctta tcgaaagatc
     6601 ggaaaaaata aatatgcaga cggtgggtat cgcaacaaaa tacctattga tattgcgatt
     6661 aacaaaggag caacagaggc atttgtcgta gatgtgcaag ggccaggccc agcgaaaaaa
     6721 attcgtatgc cagacatttt tatccattgg aaatgtcaaa cattgtggac actaggcagc
     6781 tttctgcttt ttgatagcca aaggaaccaa ttaaatttac aattaggcta tctggaaatg
     6841 aaaaaacgat taggctatta ctatgggaac tggtatacat ttcattctgt taaacctgca
     6901 agaacatact ggcgaggatt tttgtcttac ttaacaaaag aaattcagtt agaattatct
     6961 tttttcaaaa gcataaaatt ttggcaaaaa ctgcgtaatt tatataagaa cagggtcgta
     7021 ccagagacat gcggattggc gatgctagaa cttttagcaa aaaaacattt tttactgccc
     7081 aatgaaatct atcaagtaga catgatgatt caaatgatct gccaacaaga tataaaaagt
     7141 atgccggatg atttattggc acaaattggt caattaagta ctgaagaatg gcgaaggtat
     7201 cgaaaatacc agcaaaaaat acaaaatgag cgcacgaaaa ctgtttattt tgattatctg
     7261 ctacaaggaa aacgcaagga tcgtttgcag catgaattgc ttaagcaacc agttgacaca
     7321 ttactgatcc tatatttata ttatctcaag gaggaacaac catggcacaa gaattttcat
     7381 atgaaatcgt agaagaaatc gctatattgt cagaaaacaa caaggggtgg cgtaaagaac
     7441 tgaacctagt cagctggaat ggtcgcccgc caaaattcga tcttcgagat tgggcgccag
     7501 accatgaaaa aatggggaaa ggcctgacat tgacgaacga agagtttgaa caactgcaaa
     7561 aagcaataga aaatatgtaa tgaggaagga ccctgtcgaa tgaaaagtat gactggtttt
     7621 ggcaaagcca tccgcgaaac aaaagcgtat cagttagata ttgaaataaa aagtgtcaac
     7681 cagcgttttc tagatgttca aatacgaagt cctaaaatcc tgaactttgt tgaaaacgat
     7741 tttcgtcagc ttattaaaca gcagttaagc cgcggtcgaa tcgaagtatt tgtgaatcta
     7801 gcttatattg gcaaaaacga aaaacagatc gtcatcaact gggaattgat tgattcactt
     7861 atcactcaat tgacagacgg aattcaacaa cgatacggta gtcaaaatca agtgtcaatt
     7921 agtcggatgg cggaacgctt cgcgctggac gaaaacttcg taacggtaga agaaaaaccg
     7981 gaagagagca tggaagaatt ggcacagtta gctctagata cattgaaaag cgcacttgat
     8041 tctattgagg cgagtcgaga aaaagaaggt caagctttag ctttagtgtt gaaaaagaat
     8101 actgaagaat tcaaagaagt actaaaaaaa ctcgatagtt ttgtagaaat ctatgaaaag
     8161 gaatttcaaa taaggtatca aaaaaagctg gaggaatttc ttggagcgaa agtcgatgag
     8221 caacggttgt tgacggagct agccatcttg ttggaacgcg gagatattca cgaggaactg
     8281 gatcgtctgg cgatccatgt acaaaaacta gatgaactta tcgaagcaca atgtcctgta
     8341 ggtcgggaat tagatttcct gatccaagaa atgaatcggg aaatcaacac gatcggttcc
     8401 aaatcaaatg caatcgaaat taaaaatcaa gtcgtccagt ctaagacgat cttagaaaaa
     8461 atcagagaac aagtacaaaa tattgaatga cgaaagaggt agagataatg aaatattatg
     8521 acgtaacgtt ccacgaatta agtggaaaaa ctgtggttaa acgggatatc ccttctgaaa
     8581 aaaacggctt tgacgtatgg aaagatgcct gtgcagacta taatgagaac gaactgttta
     8641 ttttgatcaa cgacggtgct tacgtgacga tgaatcggaa atttattgtt cgaattgaca
     8701 cagaagaggt agaagatccg acagaaaaag cacgttctcg aaaagatgag atcatgggtg
     8761 tagtgaatac tttgtccaat atgggctttt agcaaggatg ctgaacaaag aaatacaata
     8821 aataatcaga attggatttc ttcgcttagc ttctttgctc taggaataca tttaaaggtt
     8881 gtgacaaaat tcgtgtcaca atcttttttt attttgaata agtgatgttc aaaaaccatt
     8941 ttctcggtat
//
LOCUS       DAIGOK010000106         8800 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.107, whole genome shotgun sequence.
ACCESSION   DAIGOK010000106 DAIGOK010000000
VERSION     DAIGOK010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.107"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(256..2637)
                     /locus_tag="NOA01_002138"
     CDS             complement(256..2637)
                     /locus_tag="NOA01_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HCI0633320.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2627..3274)
                     /gene="recU"
                     /locus_tag="NOA01_002139"
     CDS             complement(2627..3274)
                     /gene="recU"
                     /locus_tag="NOA01_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HCI0633321.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3338..3877
                     /locus_tag="NOA01_002140"
     CDS             3338..3877
                     /locus_tag="NOA01_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HCI0633322.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3967..4386
                     /gene="gpsB"
                     /locus_tag="NOA01_002141"
     CDS             3967..4386
                     /gene="gpsB"
                     /locus_tag="NOA01_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HCI0633323.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4408..4775
                     /gene="rnpB"
                     /locus_tag="NOA01_002142"
     ncRNA           4408..4775
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="NOA01_002142"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4984..6150
                     /locus_tag="NOA01_002143"
     CDS             4984..6150
                     /locus_tag="NOA01_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HCI0633324.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6196..7692
                     /locus_tag="NOA01_002144"
     CDS             6196..7692
                     /locus_tag="NOA01_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HCI0633325.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7773..8456
                     /locus_tag="NOA01_002145"
     CDS             7773..8456
                     /locus_tag="NOA01_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCI0633326.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         2775 a         1586 c         1864 g         2575 t
ORIGIN      
        1 gaagttgcac taaataaaag actagtgtgt atttagccga tttaattata aacaagtaaa
       61 tggataaagt atatgtaatg ataaaagaaa aatggatttt ctaattttta agctaaatgt
      121 tagatcacac atgtatatgc tggatagtct tataaaaaat catgtaatac agcttggtac
      181 atgattttta tttgtagctg ttttttacat agacacaaaa aagaaaccac gcttgtagac
      241 gtggtttctt ttttactatc tgtttccaga agctgaggtg ctgctggatg gtgtatggtc
      301 atttgcagca tccgctccgc tagttgctgg ctcactggaa gaagcaggag gagcagatga
      361 agacggtgtc gagctttctt cggatgaaga agaaggaacc gttgttgaag acgctggctc
      421 gcttgtttct gctgtcgtgc tttcttttga ggactcagca gttgattcgc tttgactagt
      481 agagctagag ctttgagtcg ttgtctctgt tgttgagctt ccaggagtct ggacatagct
      541 ggaagatgga atagttgttg atggtgtaat tgcattcgat ctagctgtat attgatcttt
      601 gtagtacagt tcaccgccca cacgaattaa tccgcttggc atctcccagt ctgtatttgt
      661 cacgttagct gaaacatact gcataagctc gcgataaaca tcagaggcta cgtgtgacga
      721 ttctgaagtg acaggtgtca tttttttatt gtaacctgtc cacacggaaa tcgaataatt
      781 tggcgtatag cccgcaaata ggatatctgg atacacccca gaagaaatac caagttttgc
      841 atactcgtca tctgtgtaat tggatgttcc ggtcttaccg gcttgatata acccagcgat
      901 ttgcgcattc gtaccagttc cttcagtgat cgtatctttt aaaatgtctg tgatcatata
      961 agctgtttct ggagacatgg ctgtttttcc atctggctca taagtttctt ctgttccgtc
     1021 ttggaagacg attttattaa catattgagg tttgtaataa gtccctccat tggcgaaagc
     1081 cgcataggct gctgccattt ttaaggaaga agctccgtat tttgtcccat cttgttcttc
     1141 cgtattacta gagatcgcat tcgattgatg gatcgtactg tattcgatcc caagattttt
     1201 taagaaagaa gccactttat ctgaaccgac ttcattgaat aatttgactg ctggaacatt
     1261 acgggataag taaagtgctt ggcgtaaagt aatggtgccc atatattgat tatcccagtt
     1321 tcctacagga gtcgaagtcc cttcatagtt atacggtgcg tcagtaatag tttttccagt
     1381 agaatatttc aaatattcaa aagctggtcc gtaatctgtg acaggtttca tcgtagaacc
     1441 aaaatcacgg gaagtattca ctgctaagtt gttgcctaaa gttacgtctt ctgcgatgtg
     1501 acgtccgcca atctgggctt ttactttccc ggtattggtg tcgataaggg tggaggcaac
     1561 ttgcatctcg tcgtctgggt aagagacata ctgatccgtg ttgacgatat catatagttt
     1621 cttttgggcg tccagatcca aattcgtata aatctctaaa ccgtctgtgt agacattctt
     1681 atctgttttt tcttgtactt cattgatgac ttctttgaca taattatcga cgatcttagt
     1741 attgtcatcg gattgagtta gttcttgcag accatccgtt accggtacat taacagcttg
     1801 atcatattct gtttgagaga ttttttcgtt ttgaagcatc gtatacaaaa cagtatctcg
     1861 tcttttcttc gcctgatctg gataaacata aggatcatag gcggaagggg cttgaggcat
     1921 cccagcaagc aatgctgttt gcggtaaaga aagttctgat aatttcttac caaagtacat
     1981 ctcagaagct gtctccatcc cgtacaagcc gtttgacata tacactttat ttacgtagta
     2041 ggtcaagatc tcttgtttcg attttttttg ttccaatctt acagccatcc acgcttcttg
     2101 ggccttgcgt ttcagagtct gatcctcggc actggtggag aagaaggaaa gtttgatcaa
     2161 ttgctgtgtc aaagtacttc cgccttgcag tccgccagag gtaaagttag agagggcaga
     2221 accgatgatc cgaatagggt caaccccgat gtgtttataa aaacggcgat cttctaccga
     2281 cacgatcgca tcttctaatg ttttcggtaa ttcattcgca gaaatttttt cacgcttttc
     2341 tgcgcctaaa tcttcgaata gttcgccatc ttgtgtatat agtttggaag atacggtagc
     2401 gtctaatttt ttatccgtca attcgggcgc atcttttgcg taataccaga aaagtcctac
     2461 acctgctaaa aagaggatac agaaaaatga gagtattcct aaaaggatct tgataaataa
     2521 tcctttttta tgtgttccag aggactttcc agaacctttg ccagaattct tcttcacact
     2581 ctttttagga gtaggctgtt tctgtcgtct agagcttcta gtttgttcat ttgccattct
     2641 tgctttctcc ttcaatagat aatgttgata aatagaagtc aaccgcgtcc aaataaggaa
     2701 tccgcggaga aataccagtt ttgatctcta ttcctagttc ttcgatcaaa gataaaggta
     2761 gtgatttgcg tcctttttcc ttttgggcgt cccagtaagc aatcaaatga acactattca
     2821 aataaaaaca gcgcttcaaa gacgaaaacc ataacaagac aaagcagacc ccattttgtt
     2881 cgaggcatgc tttcatatgt tcgatttgat gggcatggaa gtttttaaag ggaaaagatg
     2941 ttttgttctt ggtttctttt gcttcaaagt ccaagtaaaa gcctcgatag acgccattat
     3001 agtctgtagt tgaggcttgg gtaaagtagg cttccttgat cacagcagca cttctcttag
     3061 gatagtctac tttcacgatt tggatagggg taggcttttt atggataacc gccaacccgc
     3121 gggaaagata atatttatta ctttcattga ttgcttcttc aaagcgcatc ccccgattgc
     3181 cgaattcagt gatctgattt cttttttctt gaattttttc gacgggagag gcattcttat
     3241 aataaggttg gccatttgga tagcgcaata ccatctttaa tcacactcct aggccacatt
     3301 atagcaaaaa agcggataga aagaaaagga atctgagatg gataagtcaa aagtaatcta
     3361 tgtgtctggg tatcgaagtt tcgaactcgg tgtttttaaa gaaaatgatc ctaaaatcca
     3421 agttgttaaa aatgttttaa aaaaagaact ccatcagtta gcagaagaag ggctggaatg
     3481 ggtacttgtc agtgggaatt tgggcgtcga actatgggct gtgcaaatag tagcggaatt
     3541 gaaagaagaa tatccggaac tatctcttgg tattttgtat cctttttctg attttggtaa
     3601 taactggaat gaagggaacc aagaaaaaaa acaattagca gaacaattag cagattatgt
     3661 cgaagctgtt agccatcaac cctaccaatc acctagtcag ctaaagaatc acactcgttt
     3721 tatacttgag catacagtgg gctgtctgct gatctatgat gaagaattcc ctggaaaaac
     3781 gaagtatttt tttgaagatg cacagaatta ccaagcagat catccctacg aaattcggct
     3841 gatcagtatg gacgatcttc aaaattttag tgaataatcg atattgtgtt tgatttatca
     3901 acttttttcc tatacaatga aaaaggtaga aatgttatta cgtaaatgaa aactagaggt
     3961 gtaaatatgg cgaatttggt ttatagtcct aaagacattt tacaacaaga attcaaaaca
     4021 aaaatgcgcg gctatgatcc cgttgaagtt gatgaattct tagacaatat catcaaagat
     4081 tatgaaactt acagcaaaga actccttgct ttacaagaag aaaacgatcg attgagtgca
     4141 aaagtagctc aattatctaa aactcaagga gcagctcaaa cacgcgtgca acaaacagag
     4201 gcaccaaaga gcgcagcagt gaccaatttt gatatcttaa aacgcttgtc aaatcttgaa
     4261 cgtgaagtat ttggtaaaaa gcttgatcaa caagcaagtg cagtcaaacc agcacaacct
     4321 aatccaaata actacacaaa tgcagacaca agcttagatg ataatgaaaa aacacgtcaa
     4381 ttttaatttt tgattaatga atttgcggtt ttcgggtaat cgcggtctga cttgatcagg
     4441 ctgaggaaag tccatgctcg cacaggctgt gatgcctgta gtgttcgtgc ttagcgaaac
     4501 cataagctaa ggtactcttt ggagtaacgg caggaaaaca agctaaggct tcggctatgc
     4561 ttaagtatcc ttgaaagtgc cacagtgacg aagcggctgg ggaaacccat ccggtggaac
     4621 gcggtaaacc cctcgagcga gcaacccaaa caatggtagg ggcgcttttt gtctggaaga
     4681 agaacaggac aaaaaggcag tataactgca gatagatggt taccgctttg ttttttctcc
     4741 ctggaaaagc aaagtacaga acatggctta tagaaaaccg caaacatcag ggaattaggt
     4801 cgtgacaaaa gtaatgcgtt ataagaaaca agaaaaacat ttgaaagcta tttttcaata
     4861 cttcagatga atcgttctta ttacaaaaca aacttttgta tcaccggtta ttcggaattt
     4921 gagtaaaagt gtggggtagg actgatgtcc cacctccttt ttttataaat aaggagattg
     4981 tgtatggaaa aaaacaggaa gttcaacctc gtcgcaactg cggcaagcgg gctagaagca
     5041 ctggttggta aagaattaag agatcttgga atcgaatgcc aagttgagaa cggacgtgca
     5101 cgttttgaag gaacaatgga aacgatcgct acagctaatt tatggttacg tacagcagat
     5161 cgagtaaaaa tcgtggttgg ggaattcgat gcgtatacgt ttgatgaatt gtttgaaaac
     5221 gtaaaagcat tgccttggga agattttctt ccactagatg cagcttttcc tgtagctgga
     5281 aaatcgatca agtcgaaact atatagcaca cctgattgcc aagcgatcac aaaaaaagca
     5341 atcgttgaac gtctccgtac ctattatcac cgtccagctt ctgtaccact gacggaaact
     5401 ggcgcacatt ttcagttgga agtagctttg ctgaaagacc atgtgatggt aactctggat
     5461 acgacaggac caagtttatt caaacgtggt tatcgcttag aaaaaggtgg agcaccattg
     5521 aaagaaaaca tggcagcagc acttattatg ttgacaaatt ggcgaaaaga ccgtccattt
     5581 tatgatcctg tatgtggttc aggtacgctt tgtattgaag cagccttaat aggccataat
     5641 atcgcaccag gattcaatcg tgagtttgct tgcgaatctt gggattggtt cagtcaagaa
     5701 atcatggaaa atgttcgatc agcagccgaa gaaaaagcag attatgatat tgaattagat
     5761 attactggtt cagatatcaa tggacggatg attgaaattg ccaaagccaa tgcagaagaa
     5821 atcggattag gctcttcgat tacctttaag caacaagcag tcaaagactt caaaacggat
     5881 aaagaatacg gggtcatcgt ggctaatccg ccttacggag aacgtctagg agaagaagaa
     5941 actgttcgga aattatataa agaaatgggc gatgtattcc gtccattaaa aacatggagt
     6001 aaatatatct tgactagcga cttagcgttt gaggaatttt acggacaaaa agcaaccaaa
     6061 aaaagaaaat tgtacaatgg tgcattgcgt acggatctat tccagtattg gggaactcgt
     6121 ccgcctagaa aaaaagagaa agacgaataa tcttactaat tgattcattc aattggatgt
     6181 ttggaggaat agggaatgaa agaaaaagag tttttacaag aagtaaaaga aatcaatctt
     6241 ctacaacaag ctttaggaat cctcgattgg gatacacaaa caggtatgcc tgaaaaagct
     6301 agtgcatacc gaagtgaagt agacagctat ctttacagca tttatttttc aaaaaaaatc
     6361 ggtccaaaaa tcaaagaagc cattcgctat ttttctgaac atccggaaga attgtcggaa
     6421 gttggaactg ccgtttttgc caaagtaaaa gaagagtatg atttggaaca tcgcgtaccg
     6481 gaagatctta tgagaaaata tacagctgcc gtttcttcag cacacggaca atggcaaaaa
     6541 gctagagaaa cacaacaatt ttctgatttt caacacgcat tgaatcaaaa tattgaatta
     6601 acaaaacagt tgatacctta ctggagaaaa gatgaaaaga cgaattatga tgttttgctg
     6661 aatcaatacg aacccgggat gactgtcgaa atccttgata aggtatttga tcagcttaaa
     6721 gaaggaattc tttcgatccg ccgtacgctc caagaaaaag ggactgaacc tgaaacagac
     6781 tttttaaatc ggagaatgac gaaaaaacaa cagaagagat ttgtagtgaa agtaatcgaa
     6841 caacttggtt atgattttag tcgtggtcgt ttagatgaca ctgtccaccc atttatgact
     6901 ggaattaatc cgaatgatgc acgaatcacg acacgatgga acgaaacaga tttcaaaatg
     6961 gctgtttttg gagtgatcca cgaggctggt catggtatgt atgaacaaga catcgatgat
     7021 aaatatgcgt atacaccggt ttatgaaggt acatcaatgg gtatccatga atcacaatcg
     7081 ttgtttaacg agatcattat tggaagtagt cgcagcttct ggaaaaagca atatcctttc
     7141 tttcaagaat gtgcagaagg aacattcgat gatattcctt ttgacaaatt ttatcgttca
     7201 ttgaaccata caaaatcaag tttggttcgt atcgaggcag acagtttgac ttaccctctc
     7261 catatcatca ttcgttatga aatcgaaaaa atgattttca atgaaggggt agatgttgct
     7321 gaactacctg aaatatggaa tcaaaaatat gaagagtatc tgggaatacg accaacaaat
     7381 gatgtggaag gcatcctaca agacgttcat tggtcaggtg caagctttgg ttattttccc
     7441 tcttatgcat tagggtacat gtatgcggca caattacttc attctttgca gcaagaaata
     7501 gatgtggaaa atgttctagc gtcagacgat tattctccaa tcaaaaactg gatgtcagag
     7561 catatccatc aatatggtgc atctagaaaa ccaaatcaat taatcatgga tgcaacacat
     7621 gaaccgctta atccgcaata cctgatcgac tatatgaaag acctgtattt taatgtatat
     7681 caggtaaaat aaaatacaat ttacaaagat agctgaaatt catctaatct atagatgttt
     7741 tcagctattt acttaagaaa gaagtggaac gaatggataa attacaagtg atcgctgatt
     7801 atagtatgga ccaattagcg catgatcaaa caggacatgg gagcgatcat accaaaagag
     7861 ttgtcaagtt agctgaaaaa attttggaga cggaaccgca ggcagaccgc tttgtgacgc
     7921 ttgcagcagc ttacctacat gatacgattg atgataaagt cgttaaaaat gagaacgagg
     7981 caaagcaaca gcttcgtgta ttccttcgct ctttgccaat agtagaggaa cagatctcga
     8041 tgattttttc cattatcgaa aatatgtcct tttctaaaaa tctctctgaa gcggccgaac
     8101 tatctctgga aggtaagatc gttcaagacg ctgatcgtat cgaagcatta ggagcaatcg
     8161 gcattttgcg tactgcttat ttcggcggtg gacatgggca cccgattttt gatcctgaac
     8221 tgtatccgca aacattcaag gacaaagaaa attaccgaaa aggcacgaca gtgatcaatc
     8281 atttttatga aaaactgttc ttgctgtctg ataaaatgaa tactgattac ggatatgaag
     8341 aagccaaacg aagagaaacc ttcatgcgtg aattcttaga agaatttttt ctggaatggt
     8401 ctattgacga tgaaaatttt actccagaca attggatcaa aaataacaaa aaatgatgga
     8461 ggttgtgaca gaagtggtca gctccaagat atttcggatc attttccgta aaagttactt
     8521 ctggaacact gtttaccaag aggtcgtgag cctgccgttc agctccgagt cacaaggaac
     8581 aatttcaaaa aatagcttct cgtgtttgat gaatattgta acttgtgatt cggaattggc
     8641 aggagaacac cgtttattcg gaagaatagg actgtgacaa gacttttgtc acagtccttt
     8701 tttcgaattc aacaagtaaa acttttaaga aatattcaga gagtaaaatt aaaaattgct
     8761 gataaacgaa gatggaaatt ttatgtattt ttattggtct
//
LOCUS       DAIGOK010000107         8652 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.108, whole genome shotgun sequence.
ACCESSION   DAIGOK010000107 DAIGOK010000000
VERSION     DAIGOK010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.108"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..308
                     /locus_tag="NOA01_002146"
     CDS             <1..308
                     /locus_tag="NOA01_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCI0633327.1"
                     /translation="HHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGL
                     IALQEQDFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            338..460
                     /locus_tag="NOA01_002147"
     CDS             338..460
                     /locus_tag="NOA01_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCI0633328.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            478..2001
                     /locus_tag="NOA01_002148"
     CDS             478..2001
                     /locus_tag="NOA01_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HCI0633329.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            2025..2264
                     /locus_tag="NOA01_002149"
     CDS             2025..2264
                     /locus_tag="NOA01_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCI0633330.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(2457..2918)
                     /locus_tag="NOA01_002150"
     CDS             complement(2457..2918)
                     /locus_tag="NOA01_002150"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCI0633331.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(3113..3331)
                     /locus_tag="NOA01_002151"
     CDS             complement(3113..3331)
                     /locus_tag="NOA01_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCI0633332.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            3449..5065
                     /locus_tag="NOA01_002152"
     CDS             3449..5065
                     /locus_tag="NOA01_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HCI0633333.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            complement(5306..6646)
                     /locus_tag="NOA01_002153"
                     /pseudo
     CDS             complement(5306..6646)
                     /locus_tag="NOA01_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(6922..7077)
                     /locus_tag="NOA01_002154"
     CDS             complement(6922..7077)
                     /locus_tag="NOA01_002154"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HCI0633334.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            complement(7269..7463)
                     /locus_tag="NOA01_002155"
     CDS             complement(7269..7463)
                     /locus_tag="NOA01_002155"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCI0633335.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKC"
     gene            complement(8219..8410)
                     /locus_tag="NOA01_002156"
     CDS             complement(8219..8410)
                     /locus_tag="NOA01_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCI0633336.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            complement(8504..>8652)
                     /locus_tag="NOA01_002157"
     CDS             complement(8504..>8652)
                     /locus_tag="NOA01_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCI0633337.1"
                     /translation="KEPEQIFLIEKFIFDKTDKEIGKVLGITRQGATNLKHRLYKKIQ
                     KMLV"
BASE COUNT         2800 a         1398 c         1679 g         2775 t
ORIGIN      
        1 aacaccatca agtacatgag tttaaatcaa ttctcttaac ttcagatcaa gacataaaaa
       61 aagatttttt actccaatgg attgactggc tgattttaac caaccaaggg agcctgtatc
      121 gttttaaagg attgattgct ttacaagagc aggattttat agttgccatg caagaggtaa
      181 acgaacaggt taattttcaa tacaccactc gacaaaaaga ccaaaccact attattctaa
      241 tcggaaaaat gttggatgaa gaaaggatca aacagagttt tgataaatta ctggcggaaa
      301 gcaaataatg ataagtagtg attgggggta atttctaatg gagaaaacgg atatatctag
      361 tgcatatcga cgacttaaaa gcccgaatat taaaactaga aaaagggcat taaaaattat
      421 caaagaacat aaacgaaata aaatgaagaa attagcataa ttaaggaagg aaaaagaatg
      481 aagaaaatca gtgtaggact tattgggata gcagctctag gattattggg agcttgctcc
      541 agcactaatg atgcaaaagt atcgaatgat aaagatggaa aattagaaat tgtaacaaca
      601 ttctatccaa tgtatgattt caccaaaaac atcgtcggcg atgaagcgaa cgtggattta
      661 atggttccag cgggatcaga acctcatgac tatgagccct cagctaagga tatggcaaaa
      721 gctcatgatg ctgatgtttt tgtatatcat aacgaaaaca tggaatcatg ggtaccaaaa
      781 gctaaagaaa gttggaagaa agctgggcct aatgtagtag aaggaacaaa agacatgatt
      841 ttattgccag gtagtgaaga agaagatcat gatcacggag aagaagatca ccatcatgaa
      901 ctagatcctc atacgtgggt ttcacccaaa atggcaatta aggaagtttc aaatatcaag
      961 gatcagttag ttaaattgta tccgaaaaaa gcaaaagtat ttgagacaaa tgctgaaaag
     1021 tatttgacta aactgaaacg gttggatgct gactatacca ctagtcttaa agaagcgaaa
     1081 caaaaaagtt ttgtgacgca acacgcagcc tttggatatt tagctttgga ttatggattg
     1141 aaacaagttc ctattgctgg actaagtcct gaagaagagc catcttctgg tcgattagct
     1201 gaattaaaag agtatgttaa aaaaaataag atcaattata tttactttga aaagaatgct
     1261 aatgataaaa ttgcaaaaac tttggccaac gaagctggga taaaattgga agtactgaat
     1321 cctcttgaaa gtcttacaaa agaacaaatg gataatggtg aagattatgt ttcagtaatg
     1381 gaagacaatc taaaagcgtt agaaaaaaca actatggttg ctggtgaaga ggtagttcca
     1441 gaaaaagaag cgaaggatga aaagacagtt gctaatgggt actttaaaga tgccgatgtg
     1501 aaggatcgcg aattgagtga ttatacaggc gaatggcaat ctgtttatcc gttgttaaaa
     1561 gatggaattt tggatgaagt tttcgattac aaagcaaaaa tcaataaaga catgacagct
     1621 gccgagtaca aagattatta tactactggc tataagacag atattgatac tatcaacatt
     1681 aaagacaaca ccattgattt tgtagtcaac ggtgaacatc atcaatacac atacaaatat
     1741 gttggataca aaattttgaa ttatgaaaaa ggaaaccgtg gtgttcgttt taattttgaa
     1801 actgatgatg caggtgctgg tcgttttaaa tatattcaat ttagtgatca tggaattgca
     1861 ccaagtaagg cagagcattt ccatattttc tttggtggag aaagtcaaga aaaactctat
     1921 aatgaattgc ataattggcc tactttctat ccagcttcat tgtctaaaca tgaaattgct
     1981 caagaaatga tggctcatta aaattcaatt tttggaggaa atacatggta agaaaagtat
     2041 tattgtctat ggattccaag aatattgatg cttatcggcg ttatcgattt gcaaagcaaa
     2101 tgagattaaa tgggagtaca ttagctgata ttgctgcaac tttacatgtt agtattgaca
     2161 cagttcatag agtattaaaa cgggatcctg attgttgttt tatcaaggtc aatactggaa
     2221 gaagtagacg tttagcaaaa cggcataata gcttggccaa ttaattgata ataccagagt
     2281 agatcatttt tcccttattt ggatttgatc tgctttttat tatgtcttga attgtcaaat
     2341 caagcgcaac actttatcaa agaagacttc ataaggtgtt ctccaatcta aacactttct
     2401 aggtctgtta ttcagttttt caatcatctg atttatcgtt gagttatcta ccgtagctat
     2461 ctctttcgat tttggcaagt attctcgaat gagtctattc gtattttaat ttgttcattg
     2521 ttgccaaggg catgaggatc tggaaaataa aagggcatat tattcaatgc ttctgtaact
     2581 gcgaaatgat tggagaattc ttttcctcta tctggagtga tcgttttgac cgcctcattt
     2641 ggaatactgc ttaaaagttc aatcattttc tcgctcacat aagcggcttt ctttctgggg
     2701 accttctttc ctgctaaaag gaagcatgat tttctgtctg tcaaagtcac taaacaagta
     2761 ccaccagttt tgccagctac aatatcggct ttccaatgac caaaatcact tctatcatct
     2821 gcggattttg gacggccatg gatcgaatga gtaaggcaaa tctttccacg agactcgaca
     2881 tgattttttg tatgtcttgt ttttcctcga tggtgcaatg aacggatcag gcctttattg
     2941 ccgtgtgaga ggggttctgt atcgaaaaga tcattgcaaa ttgcacgtta gatcgtgata
     3001 aaactaattg aaaagtccat atgttcatgc ttcaaacgat tcgagatttc ctctggcgac
     3061 cactgaaaat cgacaaataa atgacgaaca attgaccaaa ctcacggatc atttagaatg
     3121 tgataacgtc cgcatttttt tcttcgataa gcataatttt tttccgcttt ggaaggtgaa
     3181 tagttttttt cttgtttctt ctcagttcat gtaaaatagt aaacgggcta tgctgaatgt
     3241 cgttgccaat tatactaata gaaaatcctt cttcgtgaag cagaaagatt ctttctcgtt
     3301 cgagtatagt aagatgacga tactggctca taattatttt ccttctagga gtgttctcga
     3361 caaacttata ctagttggat atcgattatg actcattttt atgtgttgca cttaaagtgt
     3421 aaattcaagg tatagaaaga ggttccctat gaaaaaaata cctattacaa tcttaactgg
     3481 ctatcttggt tcaggaaaaa cgatacttct aaatcatatt ctgtacggtg atcacggaaa
     3541 ggaaattgct gtcattgtaa atgacatagg ggaagtaaat gttgatgggg tattagtcga
     3601 caagaaaggt tttaaaagaa ctggagaaaa acttgtttcg atgccgaatg gatgcatttg
     3661 ttgtacatta cgcgaagatt tgattgttga actagatcaa ttagtgcaat tttctgactt
     3721 gaatcaaatt gttattgaag cttcaggaat cagcaaacca attccaatta ttcaagcaat
     3781 tatattagct aaaaaccctt tagacattga tttgacccaa cgattagaga ttggtgccat
     3841 tattatagta gtggatgtaa aacgtttttg ggataatttc gaaacgggtg aaaagattca
     3901 ggaaagaaaa gctgaaggta atgtagttga agaagaacag gatattcgtg gcttgttaat
     3961 tgatcaattc gagtgttgta acatattact tttaaataat agtgacttag tgtcaaccaa
     4021 gacgatttac aaaattaacg gtctaattgc taagttacag tccaatgcca aaataattag
     4081 gacaattaaa ggtgaagtag acttatcgaa aattctaaat acaaatcttt ttaatataga
     4141 tgagacttcc tattcagtgg gctggttcaa agagttagag cttggctatc aaaatcatat
     4201 tcctgaaaca gaagaatatg gtatctcaag ttttatctat cgaaatcggt tactccttga
     4261 cgcacaaaaa tttgcccatc ttacggataa aaattttcct ggcaatatta caagagcaaa
     4321 agggtttctt tggtttgccc aacatagtga tttttcgacg atctttgaaa catcgggtag
     4381 ctataaaagt attgaatcat taaattattg ggtagctagt ttacctaaaa ttgagtgtca
     4441 aatgattata caaaaaaatc ccgaattgaa ggaagcctgg catccgttgt ttggtgaacg
     4501 cattaataaa ctagttttta ttggtatgaa gatggatcaa caatgtatta aggaacaact
     4561 tgagggttgt ttaactgatt ctcttaatgc agataatttc cgaaaaattt caccatttcc
     4621 atggaaaaag tctaatatgt ttagccagat atcgttaatg aaatttttaa gaaaagctgg
     4681 tgtcaagata ggcgatgtca taattattgg cattttgatt attacctctt ttattccctt
     4741 atttgtattc tctatagaaa aggagaatga aacaactgaa gatgctatca aatatgccat
     4801 cgttcgaatt gatggcaagg aaatggatcg cttcgatttg gataagatca atcataaact
     4861 cgtgaattat catcctgcga agggacaata taatattgtt gagattaaag atggaagaat
     4921 tcgagttaaa gaagataata gtcctgatca aatcgcggtt agaactggat ggatctcaaa
     4981 acccggtcaa acgagtattt gtttacccca taagttagtt attagtattg agaaaaaaga
     5041 aagcacagat tactacattt actaatctgg aaatagatga ggaggttttt aataagaaaa
     5101 agatattatt attaataata gcaggggctt tattaagtgt gttaagcgct tgtggacaag
     5161 aaaaagaagc ggtgacaaca ggcacaagta tctttaacgg gacctgcaat ggtttagtta
     5221 acagtcggtt agattactta ttttggctct tcgtcaatga gtgttggtga agaaattcaa
     5281 tagaataatc gagatactca cacggctaga cagctatctt gtctagccgt gttgttgttc
     5341 tggctttagt cagttgggta gtattcttag ttttcttaaa atattttcct cgatataaaa
     5401 agattcgttc tcggtagttt tgaaagtttc tatagccata ggcaattcgt tttagtactt
     5461 tgatatggtt attcaaacat tctatagggc cgttggaata gggaacgcgc aacgctcgtt
     5521 tgatttctgt ttggtatttc ttgaaggtcg tgaacactgg ttggtagtgc tctggcaact
     5581 ccttgtagtc ttcttttagg agcgcgacga attcaggaac atcttgtctt ctgataacgg
     5641 aaagaaaatc ctggtagact tcgtagcctc gctttaagga atcatcataa gataatagcc
     5701 gatcgactat ctctgcttct gtcagatgat ctcgaaatga cggacgccag tagcgatgct
     5761 gacctttcaa ttcccacgca ttcttttgaa gtagtttcca gtatttcttt aagtgcttcg
     5821 accgacgatc ccctttttta aaggtcttca ttacttgaat tcggtggttg gtaaaagccc
     5881 gaccaatgtg ttggctaata tgaaagcgat cggcgacgat tcgggcattc ggaaaacact
     5941 ctttcaccaa tgtgcgatag ggtgtataga aatctgaaac cactagacgc acttgttcac
     6001 ggacttttat agggtagcgc aagaaatagt cgcgtaaagg attgagttgc cgattttcca
     6061 caatatcaat cagttgatgg gtcttgccgt ccatcatgac aaagctcatg gctcccgaga
     6121 catttttcac tgatttgaat tcatcgaaac acaccacttc tggtaacagc tggctcaaag
     6181 aaatggtttt aggccgaagg ctgcgaagta ttcgaatgac agtggtcgga gaaatgtgct
     6241 tcatacgtgc gatcaaagac atagaaagag gttcgcttaa tagctcaaga atggcttgtt
     6301 tgactctgcg agcaatggag cagcgacgat cactaactga atcttctgct aaaaaagtct
     6361 ttccacagtg tttacatttg aagcgctgtt tgttaatccg cagaatggtc ttgtagttac
     6421 tgacatcatt caataagatg gatacttttt tccagcccta tttaatgatc gtttgttcat
     6481 tttgatagtg gcgatgaaag cattcctccg gttgataggt caatgtgcca tcaaaaacaa
     6541 aacatgtttc gtcattgatt cgctctttgc gaaaggcatc ttcatgaaag ataagattta
     6601 ggtctagaat atctagtaac tctttggtaa actctgaata ggacatcttg aaacactcct
     6661 ttgaatggtt ttcgtcgact ttattctaaa ggattttaag gtgtctgttt gcgtttatta
     6721 gaaaaaagaa aaagtattgg caacagaagg cgtcaccaac actagatatt atagatccct
     6781 tattttcttc gcttcaaatt gtcaaggagt catcataaat tttgtatgga ttcttttgtt
     6841 tgggtagtag tcttctaaat aatcttggat taacaccttc atctctattt tacttttata
     6901 tttgttggag tcgattaatt ctcatttgat tgatcgatga aacgcttcaa ttactgcatt
     6961 gtcaacagga gtcccagttt ggctcactga atgtgtgatg tggtggtcgt tcaacagctc
     7021 ttgataaata aaagaccgat gctggctacc tctatcggaa tgaattaccg taggcaattc
     7081 tccttaataa atttgtaatg gacggacgac aatctctttt ttcatgtgtg aatctaccat
     7141 atagtaacgt agaatacgag ttgctaagtc aatataggtg gacaaataga ggcgaccgtc
     7201 attacatgga atgtaagtaa tatccatcac ccaaacagca tcaatttggt tctgttcaaa
     7261 ttatcgattc aacatttttt taccatttct ttgattgttt tttctctacc atgagaagat
     7321 ttgcgtcgat gaaatcctct tgtgcataaa ttcatttcac gcataagaac agctacaaca
     7381 cgtttattga tttcgattcc ttcatcgtaa agtttttgag tgatccctgg agatccgtat
     7441 cgatctttgt gttcgtagaa cactcgtctt cctcttttag tcgtttattt tcctgttcaa
     7501 gaatgtagaa atctgcttta tcaggtaatc tttttccttg tccagcaaaa gcatttttcc
     7561 cgtatcgacg atattctgaa acccatcggg aaattgttgg acgagagata ttgtattatt
     7621 cagcaatttt cttggtagtt acttttttct caaggatatc ctgtgaaatc tttagtttaa
     7681 atttacgatc atatgttttt ctcaatagag ttcattcctt ccggtaacgt aactgatagt
     7741 caagaaaagt gtatcatagc ccaacttagt atcagggttt gtcaactaaa ctgtggaagt
     7801 tgatagataa gagtttttaa caaccaaaat tctcttgact atttttgaaa tcagacaaat
     7861 tttgattgga cacatagttg aataaatcta ccgtcgttac ggaaggtaaa tcgcatgctt
     7921 ttcaattcta gaggagaagt cctcgttgaa gtaccatagc ccagttctgg attcgacctc
     7981 ctgcccactt catacgtaaa taaagtaatt ttaggagtga gactgtgaca aaagttattt
     8041 ttaagctaaa aacgaattaa aaaacgttga taattaaaat cattcgtttt agattctatg
     8101 aatcattata gaacgctgta aaatcgatac tttataatta taaagaagat agtttcaaat
     8161 ttattgttcg aaatttgaaa ctatcttttt acttatgtca cagactccct tagttgagtt
     8221 attctttaat ttcgaatttc gagatagcta gatatattgc aagcatacat tcttgataac
     8281 aatcttgatc atattttcca gttctccagg attgcttatg aatcaaagag ctaaatcttt
     8341 tgagaattgc ctcaacagct tcttcatttc cagcctttgc taaaatgaaa tcttctttta
     8401 aagaattcat aaatctacct tcttcccatg gacttataat ctatttcttt gggaaggagg
     8461 agaacgagca aagaagcata caaaattagt tattatgcag atattaaacc agcatttttt
     8521 gaattttttt gtaaagtcga tgttttaaat ttgtcgcacc ctgtcttgta atccccagaa
     8581 cctttccaat ttctttatca gttttatcaa agataaattt ttcgataaga aaaatttgtt
     8641 caggttcttt ca
//
LOCUS       DAIGOK010000108         8603 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.109, whole genome shotgun sequence.
ACCESSION   DAIGOK010000108 DAIGOK010000000
VERSION     DAIGOK010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.109"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(132..1085)
                     /gene="menA"
                     /locus_tag="NOA01_002158"
     CDS             complement(132..1085)
                     /gene="menA"
                     /locus_tag="NOA01_002158"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCI0633338.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1098..2201)
                     /locus_tag="NOA01_002159"
     CDS             complement(1098..2201)
                     /locus_tag="NOA01_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HCI0633339.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2291..3247)
                     /locus_tag="NOA01_002160"
     CDS             complement(2291..3247)
                     /locus_tag="NOA01_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HCI0633340.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3528..5462
                     /locus_tag="NOA01_002161"
     CDS             3528..5462
                     /locus_tag="NOA01_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCI0633341.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5919..6335
                     /locus_tag="NOA01_002162"
     CDS             5919..6335
                     /locus_tag="NOA01_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HCI0633342.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6349..6915
                     /locus_tag="NOA01_002163"
     CDS             6349..6915
                     /locus_tag="NOA01_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HCI0633343.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKRDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6938..7924
                     /locus_tag="NOA01_002164"
     CDS             6938..7924
                     /locus_tag="NOA01_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCI0633344.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8079..>8603)
                     /gene="scm"
                     /locus_tag="NOA01_002165"
     CDS             complement(8079..>8603)
                     /gene="scm"
                     /locus_tag="NOA01_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HCI0633345.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
BASE COUNT         2527 a         1657 c         1751 g         2668 t
ORIGIN      
        1 tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat
       61 aaggagctat ttttatttgg ctgttcttat tacttgatgc agggcagttt tttcacaacc
      121 tcttcatgct gttaaatggc ttgcataatg attgttagta atagccccaa tacataactg
      181 gcattgaata agaccatatt tttgatggaa tagctgaaac tgataggctg gggtaacgtt
      241 tgcttgaatt gatccaaatg cttcttcaca attggcaagg tcaagaaggt gaccaagatc
      301 ggccattcat agataccgct caacaacccc attaaaatca cgaaatagca agcatacatc
      361 aatagctgga ataagactgt tcccgcagga cgtccgatat aaaaaactag tgtatagcga
      421 tgattttcaa tatcacggtc aagatcccgc aaattattag ccaacatgat attggcaatc
      481 gtaaacacca agggtaagga agctaatacg attgctgcta tagcccaaag atttcctacc
      541 aaatcaaatt gtccagatga aaagttgact gtcagatcat agacatctga accgaatgta
      601 ttgacataaa cgaccatggc aaaaattcca aggcccatag tcaccccact gaaaatttcc
      661 cctaaaggca ttcgagataa aggaatcggt ccgaatgtat agaatactcc aataaaacaa
      721 cagataaatc ccatcaaaag taaaagccaa cccgtattga tcgttagaat aacgccgata
      781 acaaaaacaa atgcaatcat agctaaaatc atgtttcgaa tcaatacagg agatagattt
      841 ctgcggccaa tgacattttc ttcatactta tatacttcag aatgagcctt ttcataatcc
      901 atgtaattat tgatagcggt agtcgctaaa tcaaaaacta gcattccaat aaaaaataaa
      961 aaagtgttta gccaatgtat ctcatgaaaa aaagcgatag agaataatac ccctaccgca
     1021 aaaggaaata cactagccac ctttgttcgt atttcaacta cttctaaaaa gatttttagt
     1081 gtcatggttc gtactcctta ttttagttca gaacggtcac ccatcgtgta atgggagtcc
     1141 tccgataatt tgaaggtgtc tttgattgga tcggtcacat atactttatc gtcttttgtc
     1201 acgaagatcg cttccgtccc gttattcaat tcgctttcga cgtattccag tccttttttc
     1261 acacccattg aaaagactgc tgtagacaac ccgtcaccat caattgattt atctgttatg
     1321 atcgtgacac ttgcgatatc attgtcgaac ggataacccg tttttggatc aaacaaatga
     1381 tggtattttt tgccatctac ttctaaataa cgctcgtaaa ttccagaggt aactaatgtt
     1441 ttgttccgtt ctttaataga acctaaaaca gatccgcgag ccatgttcgg atcttggatg
     1501 ccgactgtcc agtcttggtt ttcgcctctt gggctgtggc ctaagacata gacattaccg
     1561 cccaagtcaa cgatggcagt ggtcactcct tgttttttca agactttcac tacttcgtcc
     1621 gtaatgtaac ctttggcgat tgcacctaaa tcgatgatca ttcctttttc ttttagataa
     1681 acagtttttt ctttgtcatt tagttcgatc ttatgataat ccactaattt caatgcctga
     1741 tcgatttctt cttgggaagg ttttctggcg tcatcaaaac cgattcgcca taattgtgtg
     1801 attgcaccaa tcgccatatc gaatccgcct tgagaatctt ggctatattc gtaggcccgc
     1861 ttgactaacg tgtagacgtc atcagaaact tttactggtt tgatcccagc ttgctcattc
     1921 acttcatcga tctcagaacc tttttgattg atcgtgattt tatcaccaag ttcttttact
     1981 cgatcgaagg caggtttcaa tgctgattct ttgccttcat catacacacg gatacgaacg
     2041 taagtcccta acaagaactg ttcatcagaa tatggatctt tcaatatctt ggtagctgct
     2101 gaactttcag tggttgtttt atttgctgag ttgctgcagc cagttagcac cataaaagag
     2161 atgcccagtg tgattaagga aaatagtagt tttttccgca tctttttcct ctcttcattt
     2221 tgaaaggtga gacgactgcc taatctggct atcgtcccac cctccaactt ttattagtat
     2281 gattatgtga ttatttttct gttacgatat tatctacttc gattgtttga gtgtcgcctt
     2341 tttcagctgc attcactaat tgttgtgcgt acatgatgaa tgaatgtgag ctgtgtgtag
     2401 cacctgtaac aacttctaca ttagcaggtg agccatcttc ttcgcccatt gctttgacga
     2461 attcgtcgtt cagagtaggg atgtaagttt ttggttctgt accgcttttt gctttcatat
     2521 tttcattata ttctgtatca tcttgttttg atttaccgtc tttgtcgata taatcaaagt
     2581 ttgattcagt tactttgccg ccagcaactg tcatttcgaa ttgcacacgg tagccgttag
     2641 agtagttctt ttcttcaagc ttgtaagtgc cgtctttcaa atcagcacca ttgtcgattt
     2701 cgattgtatc tgtgttacca gcttgagcag cttggatcaa ttgttgtgcg tagttttgga
     2761 agctttcaga tgagtgagta gctccagtta cgacttccac accgcttgcg ctttgtgctt
     2821 ttaagaattg gtcattcaac tcaggaatgt atttttctgg gtttgtgcca gattttgctt
     2881 ccatgttttt gttgtattct gcatcttttg tttttgattc gccgttttca ttgacgttat
     2941 catattttga ttcagtgatt ttgccgtctt ttacaaccat ttcaaagact gcacggtaac
     3001 catttgaata atttttttct tctaatttat atgtgccgtc ttgcaaatct ccgccagcta
     3061 caacttttgc agatgattct gttgtgcttt ttgcagtcga agattgtgct gtagatgatt
     3121 cagaagaaga gtttgagctt ccgttgtcat ctgcgccgca agcgcctaac actagagctg
     3181 aaaatgctaa tacggcaaat ccagttacaa aacgtttttt cttcatttta ataccccacc
     3241 attccatttc tagtccacaa tttttctttg tgattcatta tacattctaa tctttttagc
     3301 ctataagtca actatttttg ataaataacc catgtaatat ttcaaacgtt ttctttcagc
     3361 aaatttcgtg aaaaaacgct ttattttaaa atttgaaaaa aatccaaaag gagttagaca
     3421 aaagaacaga acatctatat aatatatgga gattgtgtaa taagtatcaa actaaaagaa
     3481 ttatcatata ttcattatat agatagtcta taaggagcgg atgatcaatg gcaaagcaaa
     3541 atattgtcgt tgtaggagca ggatatgccg gtgtttcagc tacaaaatac ttggcaaaga
     3601 aactgaaaaa agaagatgta gcaattactc tgatcgatcg tcattcgtac catactatga
     3661 tgaccgagtt gcatgaagta gctggcggac gtgtagaacc agaagcaatc caatacgatc
     3721 tgcagcattt gtttgctcgt caaaagaatg tacaacttgt cacagacact gtaatcggta
     3781 tcgataaaga acgaaaaatc gtcaagacca aattaggttc ctatcctttt gatcagctga
     3841 tccttggaat gggcggagag ccgaatgact ttggtacacc tggtgtcaaa gcacacggat
     3901 tcaccctttg gtcatttgaa gattctgtaa ggattcggga acatattgag aagacagtag
     3961 caaaagctgc attggaacca gatgatgaac tgcgtaaagc catgctgact ttcgttgtct
     4021 gtggttctgg atttaccggt attgaaatga ttggggaact gatcgactgg aaaaatcgcc
     4081 tagccaaaga ttataaatta gatccaaacg aaatcacact gatggtcgta gaggcaatgc
     4141 cgacgatctt gaatatgttg gatcgaaatg atgctgcaaa agctgaacgc tatttgcgta
     4201 agcacaacgt ggaattatta ttgaatgcac caatcgtaga agtggctgct gatcatatca
     4261 aattgaaaga cggacgcaac attccgactc acacactgat ttggacagct ggtgtcaaag
     4321 gaacttctga cgctgctgac tttggattag aatcagcacg cggacagcgg ttgatcgcca
     4381 atgaatatat gcaggcaaaa ggctacgaag ataaaaacat ctatatcatt ggtgatttag
     4441 tcttttatga agaattccca ggaacaccga caccgcagat cgttcaagct gctgaacaaa
     4501 caggacacac agccgcagcg aatatcgtgg cttcgatcaa aggcggagaa aagcataaat
     4561 ttaaaggaaa ctatcaagga tttatggttt ctattggtgc caaatggggg gtagctaacc
     4621 tatttgataa gatccattta agcggatttt tagctatcgt aatgaaacat atcgtgaatt
     4681 tgaaatactt ctttgatatt cgttctggtt attatatgtt ccaatattta atgcatgagt
     4741 ttttccatat caaagatgat cgcaacgtga ctcgaggaca ttcttctcgt tacggcaatg
     4801 tcttatggag tgtgccactt cgtattttct atggtttagt ttggttcgtt gaagcttcca
     4861 aaaaaatcat tgggaatggt gaagtgttca aaccaagtac ttggtttggt gaaggatcat
     4921 ggttcactgg aaacagtgtg ttcccttgga catgggaata caatgtaaca gatgttgcca
     4981 gtggtgcttc tgctgctggt gatgcaacca cagctgccag cggagcaggg gcaagtgccg
     5041 gagaagcagc gactcaagct gcacattttg gtttgagtta tgcatacggt gaagaaccaa
     5101 tggctgtgat tgagaagtca ccagactggt tcacaaagat catgcaagtg atcatgccaa
     5161 acaatgatgt agcaatgttc ttccaaaaat tcatggtatt ctttgaaatt gccttggcat
     5221 tagcattgat tgctggatta ttcacatggc tatgcagtgc gacaacgatt gtattggtga
     5281 tcacgttctg cttatcaggc atgttttact gggtaaacat ttggttcatt tttgttgctt
     5341 tcgcattgat gaacggttcc ggacgtgcga ttgggttgga tcgctgggta attccatggc
     5401 tacaacgtac actaggtaat tggtggtacg gtagaccaag ttcccagtac ggcaagaaat
     5461 aaatagataa agaacgttat ttgaatgcaa gatttcaata gaaaggggct gagacaaacc
     5521 gtcacagcct cctttttatg taaaattaaa ggaataagat gcccgagata agcctccatg
     5581 agcaagacac aggtgtcaca acctctgtta caacctcttg ttataaacgg tgttccagaa
     5641 gtagcttctt cgaaaaataa gccgaaattt cacaaaaact tgaaaagtaa ttttcgtaaa
     5701 ttccttctta tttcttgaag ttgcccactt ctgtcacaac ctcttgttat aaacggtgtt
     5761 ccacctgctg cttctcggta ataagcccaa agaattagaa aacatggaaa gctgttttct
     5821 attcttcgtt cttattactc aaagcaagac acaggtgtca caacctcttg tttttatgct
     5881 aaaattgggt gagttaatct ggaaaaagga gaacaaaaat gagccttaaa gaatttgctg
     5941 aaaaaagtct gctcaaacct tgggatgttt ttattatttt gcttttgact gttctttctt
     6001 ttttaccagt catgattttt ggtcttcagc aaaacaccgc aacagcagaa aaagaagcgg
     6061 tactgcgtgt ggatggcaca gaaatcaaaa cattcccttt gatggctgga gaagagagca
     6121 acacatatac ttacaaagat gaacacggag attataactt gatcgagata gatggcgatc
     6181 gagtacggat aaaagaagca gattgcgatg accagatctg cgtacgtcga ggctgggcaa
     6241 cgaaaaatgg cgaaaccatc gtctgtttgc cacataaatt agtgatcgaa gtccgttcaa
     6301 cagatggagg tgacgaagac agtctgattt attagacctt cacacaccat gagtaaatta
     6361 caaaaaatga tttatatttc gctacttgtt gcacaaggag tgatcattgg tttattggaa
     6421 aatatgatcc cttatccttt ttcttttgcg cctggtgcaa aactaggctt agccaatcta
     6481 atcacgatcg ttgccatttt taccatgaaa aaacgagaca gcttcttact actttggcta
     6541 cgactattcc tgactacttt gctaggcgga acgatttcca cattccttta tagtatgagt
     6601 ggtgctttgc ttagttattt tggaatgtta ttggtcaagc aattaggacc aaaacgtgtc
     6661 agtatcatcg gaattagtgc ggccggcggt ttcatgcata atgtcggaca gcttttgatg
     6721 gcttctttca ttgcacaatc atggacagtc atgttgtact tgccagtcct ttcagtttta
     6781 gggattttat cagggattgc aatcgggatc gcagcgaatt atcttttacg acatgtgcag
     6841 acacttcaac gattccaaaa agattatgag cagggaacca aacaaaaatg gagcccaatg
     6901 tttaccaaaa aataatcgtc acacaaggag tttttttatg aagctacatc caatgtggga
     6961 aaactatcct gctttaagta aagaattgac agaaacacta aaacggatgg aagaaactgt
     7021 ccgcctgaaa aacaaacctg tcgaacaagc aatcaaagaa acgatccatg ccggcggaaa
     7081 gctgttgcgt cctgcctatc agctattgtt ctcacaattt ggaccagagc aagatcgaga
     7141 caaagccatc gctttggcag cttctatcga aatgctccat acggccacac tgatccatga
     7201 tgatatcgtt gatgaagcgg atctgcgacg tggtcagcct aatatccgta gtcagtttgg
     7261 taatgtcgtt tctgtctatg caggagacta tttatttgtc tgctgtttta aactattgtc
     7321 tgattactcc acttccttga aaagtctcca attgaattct cgcagtatgg aaaaagtatt
     7381 gaatggcgaa ttagggcaga tggatgatcg ctataattat gaactttctg taaaagaata
     7441 tttggaaaat atttcaggta agacagctga actgttccaa ttaagttgtt ctgttggcgc
     7501 ttttgaaagt ggaacaagcg agcgcttcgc aaaaaaagca ggagatatcg gactttctat
     7561 cgggatggcc tttcagatta tcgatgatat tttagattat acaaaagaga gccaggaaat
     7621 cggcaaacct gtactagaag acatgcgtca aggggtctat tctttaccgt tgatctactc
     7681 tttgcaaaaa aacaaaccaa agctactacc ttatttagag aagaaagcgg caatgacaga
     7741 agaagatgtc gacgccgtac gcaaaatcgt tgaacataca ggaggcgtgg aagaagctag
     7801 gaagttagcg gcctcttaca cagaaaaggc tttgaaggaa ataaagaaat tacctgccac
     7861 ctctcttcga acaaaagaga atctcttttc ccttacccaa ctgattttgg atcgcaaaga
     7921 ctgaataggg aaaggttcga tacgtaaata aatttctcag aaggaaatct acgtgttttt
     7981 tgacaaattg aattgtagtt cttgtattta tgataaaaag caaaaataaa gagcaacgtc
     8041 cgttagcgac gttgctcttt tttatcttga atattttttt accttttcga tttcttataa
     8101 ctcataaaag tgattcttaa accgattaca agtaatataa cgcctgcgag tgttatccaa
     8161 ttattcgatt gacttccagt tttaggaagt gctctttttg atttgtttcc attgtcattg
     8221 cttgttttat tagttaagtt aaagttcgtc ctgttatccg aacttgtttt tgagaaggag
     8281 acacttgcat gacgtgtatc gcttgtggta gacgacgtgt ctgtactatc gcttgaagtt
     8341 gatgaatctg agctatcact tgtggtagag gatgtatctg tactgtcact tgaagctgac
     8401 gtgtccgtgc tatcacttgt agtggtagat gaatctgagc tatcacttgt ggtagaggta
     8461 gtatctgtac tgtcacttga agctgacgtg tccgtgctat cacttgttgt ggtagatgta
     8521 tccgtactgt cgcttgaagc tgacgtgtcc gtgctatcac ttgtggtaga ggtagtatct
     8581 gtactgtcac ttgaagctga tgt
//
LOCUS       DAIGOK010000109         8438 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.110, whole genome shotgun sequence.
ACCESSION   DAIGOK010000109 DAIGOK010000000
VERSION     DAIGOK010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.110"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..461
                     /locus_tag="NOA01_002166"
     CDS             <1..461
                     /locus_tag="NOA01_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HCI0633346.1"
                     /translation="IGASIEAAQRFREFLDHMTFTVKGKTIEVYPLNHPDFNMVNWVF
                     KEQGCTDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMG
                     LTKEEWKKEQKITLLRAAIMTPYLNDDRIFNFYTKEIAKAMEKKLNEIIQ"
     gene            766..2244
                     /locus_tag="NOA01_002167"
     CDS             766..2244
                     /locus_tag="NOA01_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCI0633347.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPKKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLKNPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            2210..3541
                     /locus_tag="NOA01_002168"
     CDS             2210..3541
                     /locus_tag="NOA01_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCI0633348.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYSLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELAAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQMIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCTHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            3655..6207
                     /locus_tag="NOA01_002169"
                     /pseudo
     CDS             3655..6207
                     /locus_tag="NOA01_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
     gene            complement(6214..7651)
                     /locus_tag="NOA01_002170"
                     /pseudo
     CDS             complement(6214..7651)
                     /locus_tag="NOA01_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            7997..8359
                     /locus_tag="NOA01_002171"
     CDS             7997..8359
                     /locus_tag="NOA01_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCI0633349.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
BASE COUNT         2694 a         1384 c         1757 g         2603 t
ORIGIN      
        1 tgattggggc ttcaatcgag gcagctcaaa gattcagaga atttctcgat catatgactt
       61 ttactgttaa aggaaaaaca atcgaagtct atccattgaa tcatcctgat tttaatatgg
      121 tcaattgggt attcaaagaa caaggctgta cagatttgaa cgctatcaat gaattaaatg
      181 aaaaaatgtt cgatcggtct tcttatatgg atggcgatgt ttatggtgaa cggtttatta
      241 cttctcatac tacatttaca caagaagatt acggtgattc tccgattcgt tttgttgaaa
      301 gaatgggtct aacgaaagaa gaatggaaaa aagaacagaa aatcactctg ctacgtgcgg
      361 ctatcatgac tccttatttg aatgatgatc gaattttcaa tttttataca aaagaaatcg
      421 ccaaagcaat ggaaaagaaa ctaaacgaaa tcatccaata gaactgattt ttgtgtgaaa
      481 gaatagcaag agacatatgg agaacagaca attatttgct tcatcaaaac aattgtattt
      541 ccccctgttt ttcatatatt cctcgccaag atattctttt ttataaaacg ttctataagt
      601 tggatcgttc gtttcatgta tcaaacccag ttcactaaga tcgttcaatg gatgtttcgg
      661 tgatactggg ttttgtactg aatcgatgaa accttgtcat ttctgttctt gaacaaaaat
      721 aaaagaaaac aaaaacatat gtcgctattg aaagaaggaa taaagatgaa agatactggt
      781 tcatcaaagc aaatcacgac catgaccttt atcggaatga cctgcgccct tgtagcaagt
      841 gtacgaaata ttccagatgt agcagcaacg agctggacga tgatttttta catgctggtt
      901 gcagtattat tgtacgcttt tccaatctca ttgatctctg gtgaatttgc cggaatgttt
      961 cccaaaaaag gtggtccaga actttgggta tcgaatgctc taggaaaaaa atgggggttc
     1021 gttgtctcat ggctgttatg ggtccaaatg tttcctggga tggtgatggt cgcttcagca
     1081 cttgctccgt tgtttggtaa tattatcgat aatgtgccgc ttggtttgaa cagcaaattt
     1141 acattagttg ttattttagt cgtttattgg atcatcactt ttttgaattt gaaatttgat
     1201 atggcaaaaa tcggcggaaa agtcggtgtt tggttaggat tgtacgtgcc tttgacgatg
     1261 atgctcttat tagggtttgc tgcgtggatc aaagttggga tcgtaccaac cggaacatta
     1321 ggcagttttt catgggacaa attaatacca gatactacga ctgcatcctc atttgtttat
     1381 tttgcgccga tcatgtttat ttttactggt atcgaaatgt cctctgtgta tatcactcga
     1441 ttgaaaaatc cagttaaaac gtatattcgc ggagtatttg ccgcgttaat ttttatattt
     1501 ttcgtcaata ttttgaatgc tttagttgta gcaaatgtcg taccaaaagg acaaatggaa
     1561 ttgaataata tcgcgcaatc tatttcgatt tattgccaaa ttcttggttt gccacatctt
     1621 attgtgaatc tgtttagttt acttgtcttc attggtgtag cagtccaatt atctgcttgg
     1681 gcctcaggac cagcaaagac agtgacagaa agtgctagaa aaggtgcgta tccaccaaaa
     1741 ttcaattttt ggaagacgaa tcaatttgac gtatccaaat ccgttatttt gactcagtct
     1801 gtcattatct caatctttgc acttttttat ttgctgattc ctggcgtcaa tcaagcattt
     1861 ttgatgctcg taaattcaac tactgttatc tactgtatcg tctatgtcat catggggatc
     1921 gcagttttga gattgcgtta tacacatgca aagttgaatc gaccatttag aattggaaaa
     1981 aaagagaaaa agagcaatct tggagtttgg actgtcgtta ttgttttatt tgcggcaatc
     2041 ggattttctg tcggactgac gatgaaagct ggaacatgga tcaatctaat tgcagtcaca
     2101 gccatcagtg tcattttgtt tgtagtacca ctctgtattg aaaaaataaa gaaaccttct
     2161 tgggaacagg aagtcgccat gctgcttgaa aaagaaaaag gtgaacttga tggaaaaacc
     2221 ggcaatgaaa cggactctaa ctaatcgaaa tatccagtta atcgcattag gtggcgcaat
     2281 tggaacagga ttatttttag gttcagcaaa ggcaattgca tcagcagggc cagctgttat
     2341 tctcgtttat ttgatatgcg gtttggctat ttatagtttg atgcgggcca tgggtgattt
     2401 atttttagca aatagtacat ttaactctat cagtgatttc gttgactatt atttaggcaa
     2461 aaagtgggca ttttttgtca attggactta ttggctttgc tggcttggag ctggacttgc
     2521 ggaattagca gcaataggat tgtatatcca tttctggttt gtacatgtac cagctttgat
     2581 aagcggaggt agcgcactaa tgatcttaat ggcagtcaac cttgttgcag tcaagtggtt
     2641 tggtgaattg gaatcttttt tctcaatcat caagatccta gggattttcc tttttatctt
     2701 attgggtata ggattgagtg tctcagattt tcatacccag tctatcggaa tcaaagaaaa
     2761 ttttggacag atcgatgaat tactaccgtt tggctttgct ggaatggtag tctcatttcc
     2821 tatggtttta ttcagttttg ctggaattga aatgatcggg ttaaccgttg gcgagacaag
     2881 tgatccgcaa aagaatttga aaaaagcaat caactgtctg ccttggcgta ttctcttttt
     2941 ctatatagga acgattttgt cgattttatt tgttgttcca tggcattgtt tagatttaaa
     3001 agaaagtcct ttagtgacta tgctgcaaat gatccattgc caaggcggag cagcaattgt
     3061 caatctaatg attttggttg cttctttgtc ctcagcaaat agtgcagtct acagtactag
     3121 caggatttta tatcaaacag caaaagagaa aagtttgccc cattggtttt gtgcgctttc
     3181 taagaaaggc gtacctttac acggtatttt aattacagga atgttatttt ttgccggtct
     3241 ttgtctacat ttttttgtag ctgatagtcg attgtttttt cagcttttgg caggcatgac
     3301 aacgagttgt tttctatttg tgtggtcttc gatcttgtgt acacatgttc aatttattaa
     3361 agaaaagaaa cggaacacta cacaaagata taaagatctt gctttgttgg tgttttttac
     3421 ttcaattgct ttcagcttgt tattcgaaag cacgacacgc tttattcctt ttttattggt
     3481 tatctggttt gtattcttaa attttgtata taaaagaata agcaatagaa atatcagtta
     3541 ggcccagata cattctgcaa aacgaattat ttcagacccc cctgaaataa tcgatacgcc
     3601 tgaaaaaact tccctgcgta taataacagt atcaattaaa caggagggga taggatgaac
     3661 tggtacagat tgacggtaga agatgtattg aaaaactatc aaacatcaac agaaggcttg
     3721 tcgcaagaag aacgacagaa acgcctgact gatcaaggat ataatgaaat aaaagcaagg
     3781 cagcagacaa aacgatggaa aaaaatagca aagcacttta ctgatctgct aatgatcgtg
     3841 ttgatcattg cctctttttt gaaatttgct tctagtgaat atattgaagg aagtattatt
     3901 ttattcgtag tgatcgtcaa tggactcgtt agttactggc aagaaagaaa agcggaagaa
     3961 tcattgaatg gtttgaagca gctgatggga caagaagcga tagtactatc aaacgggatg
     4021 caagaaaaaa tccctgcgaa agaactggta aaaggagatg tcattgtctt acgtgccgga
     4081 gatgtcgttc cagcagatat ccgattaatc gaagcacatg atttattagt ggaagaatcg
     4141 attttgacgg gagaatcaga agcatctgaa aaaagtcatg ccgttttgac agaagaagaa
     4201 tcgatcggcg accagaaaaa tatggctttt tcaggaacgc ttgtacaatc tggttctgca
     4261 gtcggtgtcg ttgtagaaac aggaaacacg acagaaatcg gtaaaatcaa tcaagcattg
     4321 caataggtcg aacaacaaac gacgcctctg attaagaaaa tcaaccgatt aaacaaacaa
     4381 atttttcaag gcattctttg tttgagccta ttcttagtta tttttacgac atttcgatat
     4441 ggtatggatt ggcatatcct tctttcggct atgattgctt tggtggtgtc aatggtaccc
     4501 gaaggattgc cagctgtttt gaccatgatc ctatccgttg gagtacatga aatggcaaaa
     4561 gaaaaggcga tcatcaaagg acttccttcg gtagaaacat taggttctat gacagtgatc
     4621 tgttcagata agacaggaac attgacaaaa aatgaaatga cagttgtgga tgtagctgca
     4681 aaagaagaag cctgcgtgtt gtccattatg aaaaactgtc aggaattaaa aacaaaagaa
     4741 gaacagaaaa cagaaaattt aagtggtaat ccgacagaag ttgcgctgat tcgacacaca
     4801 gaaacagcca atctgccttt gaaaaaaata gaagcaaaga ttccgttcag ttctgaatac
     4861 aaatatatgg caacgatgca tgcggaggaa aaaggagcgg tgatttacgt caaaggtgca
     4921 tcagaagtat tatttgctaa atcaactctt tcttctgcag aacaagaagc atggagtcag
     4981 acagctgcag aatttgcgag aaaaggtcag cgtgttttag gatttgctta taaaaaagta
     5041 gattcgaaac aggaactcac gcatgagact ttaacccaat taacttttgc agggatcgcc
     5101 ggattgatcg atccgccaaa agaaagtgca gtcaaagctg taaaggaatg tcaacaagca
     5161 gggatctcag tgaaaatgat tacaggggat cacaaagaca cagcaaaagc gattgcggaa
     5221 caaattggct taaaacatac tgccaaagtt ttagaagaga tcgatttgga tcttatgtct
     5281 gatgaagagt tgattcagca agtgccaata gtcgatgtct ttgctcgaac gacgccagaa
     5341 cataaactgc gaattgttaa agctttgcag aaaaatggat aaatcgttgg aatgaccgga
     5401 gacggcgtaa atgatgcacc tgctttgaaa agatccgatg taggaattgc tatgagaatc
     5461 aaaggtagtg aagtaagcaa acaggcagct gatatggttt taggagatga taatttccat
     5521 acgattgcaa aagccgtaaa agagggccgg cggattttgg ataatttgca aaaaaccatc
     5581 aatttcttct tgccaactgc attagctcaa ggattgatct tgatttgggc gcttatgctg
     5641 aatcgcccgc tacctttgtt cccagtacag attttatggg tcaatatggt aacgacaatc
     5701 actttatctt atgcactagg gttcgagtcg ccagatccag aaatcatgaa gcgcccacca
     5761 agagatccaa agcaaggaat cctatctggt tatcatttgt ttcggatcat ctatgtctcc
     5821 ctactgatca tggttcctgc ctatttattg gctatgcagt ttgaagggca aagcttacag
     5881 caaaccattc ttctacaaaa tatcgtctta gctcaagctg tttacatgat caattgcaga
     5941 gagttgtcca agccgtcatt gaaccagggt cttttgcaaa acaaagcgct ttttctttca
     6001 ttaggtatcc ttgctttatt gcaagggatg gttatttttc taccagtcgg ccagcagctg
     6061 atcggtacag taagtttaac gatgacacaa cagttattga ttggagcaaa tgtgattctt
     6121 ctattcttag ttgtagaaat agaaaaaggg atcatgaata aactgtttcg caaaaaagaa
     6181 aaaacagcta gagaatacag tcgctagccg ttactataaa aaagttttga aagtgagttc
     6241 agtcaaacga ttgagctcat ttttcgtagg aatagaagaa atgtatacga tagggatatc
     6301 tagagaatcc agcgggaaat tggaaatcaa gatatctgtt ttttcgaaag aaatagtgga
     6361 aagatcggtt agttcgatag gtaagaagca gacacgtttt cccaggtaat cttttaattc
     6421 ctgctgaaag aatgccttcc atgaaggttc tccttggaaa ataaaataga cattgaacaa
     6481 attcggtttg atagaaaaaa tcgtttgttg gatcagtaaa gataagagtt gaaggaagaa
     6541 attcggttca ttgatttcca tttctttttt tagatcgttc agtaaattct ctttctgttg
     6601 agacaaatgt gcatattgct gatcatatgt ttctaaaaaa aatggtattg acggtccaat
     6661 gcttcaatca atatcggcga ttcatgatat tttaatacga gaagagccaa gttatcagtt
     6721 agagatgttt ttttagttga tcaagagaca gctctgaagc tagttcctct acaaacgttt
     6781 cgaggcgttc gaaaaatgga gaatgatgca ggcagcaagc ggtttcttgt ccatagttat
     6841 tgttgtaatt ccaactttcc agaaagcaga aagaagcaaa agaaagctca gtgtctttga
     6901 gataaatacc ttccgttaaa tacaattttt tcaatgcaga gtcatacagg gaggagagtg
     6961 tactgttgat atggatagaa gaatctaatc ggcaattcgc ttttatacgg atcgtattga
     7021 taaagaacca acatgttcca aaaatctctt ctgtttcaac cgctagctcc gttcgattta
     7081 ttgtttttaa aaattgaaaa taatgggatt catgaatcga ataatcttcg aaaaaatagt
     7141 tgttatgagt aaaaggaatc agcagtcgat gatagaaagt gcgaatcgca cgctctttcc
     7201 caagaataga agcggtatat ttcgtcaaat cgatctgcaa gtcgtaactt gctaaacgct
     7261 gattgatttt agtcacatgt cttttcaatg tcccataaga aagcccatgg tcggctagaa
     7321 aatcagggac agcaactttt tttgtaaaaa gcagactttt caatagttcg acactgactg
     7381 attgtttgaa aaaaatctcc caaaggtcat ttgctaataa attttgctgg ttttgcaaac
     7441 taatccctgc ttgatcgcta atgatgtccc agccttcagg cagttgagag cgaatagatt
     7501 gaaggtcaga ataaatcgtt cgttcggttg tccctatgat ttcagctaat tcatttacgg
     7561 ttatctttgt atgattcagc aattgttcta ctaaagaaac atgacgtcga atatcttttt
     7621 cggtaatgat ttttttcatt agtgaataca tcaatcagac tcccttctat tctaagaata
     7681 cggagaaaat aaacggtggt caagtttcaa ctgatttttc aatcattgtt tatttttctg
     7741 gagcatttac tgatgataag aatttcatca tgaacttgaa aaatgatacc actaaaatca
     7801 aaaaataggt gggcaaacaa taatcaacat tttaatagta gatgatagca agaccaacac
     7861 aggcggattg aacatttaag agttgataaa aaagaaaaat atcgaagtat ttgattagca
     7921 gctacttgta ttcatttttc tattcttacg tggtatcttt gaataaagcg aatcaataag
     7981 aaagagatgg aagcagatgc taacgaaaaa agaactaatc gatcacatca atgaaacata
     8041 caatttgtct ccagaatata tttttaaaaa atttcctgat tattgcgtat tcagacatca
     8101 aaaaagcaaa aaatggtttg gggtcctgat gtctgttcca gaagaaaaaa tctatgatga
     8161 tgcaagtgac cataggatca tcaatattct tgatttgaag gtcgatcctg aacttggcgg
     8221 aattttgcga ggaaaagagg ggtattttcc agcctatcac atgaataaag aacattggat
     8281 ctctattgat ctacgtaaaa tagaaaagct agatgaacta gcagatttga ttgatggaag
     8341 ttttaaatca acgcaatgaa tgtctaggac aatagagaag aggttgtgac agaaatgttt
     8401 agcttcaaga aataagaagg aatttacgaa aattgctt
//
LOCUS       DAIGOK010000110         8183 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.111, whole genome shotgun sequence.
ACCESSION   DAIGOK010000110 DAIGOK010000000
VERSION     DAIGOK010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.111"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(<1..661)
                     /locus_tag="NOA01_002172"
     CDS             complement(<1..661)
                     /locus_tag="NOA01_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCI0633350.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EE"
     gene            722..1462
                     /locus_tag="NOA01_002173"
     CDS             722..1462
                     /locus_tag="NOA01_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCI0633351.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(1548..2306)
                     /locus_tag="NOA01_002174"
     CDS             complement(1548..2306)
                     /locus_tag="NOA01_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCI0633352.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(2303..3331)
                     /locus_tag="NOA01_002175"
                     /pseudo
     CDS             complement(2303..3331)
                     /locus_tag="NOA01_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(3351..6518)
                     /locus_tag="NOA01_002176"
     CDS             complement(3351..6518)
                     /locus_tag="NOA01_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633353.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(6742..7626)
                     /locus_tag="NOA01_002177"
     CDS             complement(6742..7626)
                     /locus_tag="NOA01_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCI0633354.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(7721..8158)
                     /locus_tag="NOA01_002178"
     CDS             complement(7721..8158)
                     /locus_tag="NOA01_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633355.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
BASE COUNT         2353 a         1730 c         1408 g         2692 t
ORIGIN      
        1 attcctctag atgatggtaa tattcttcat ttatttgata cagttgaagc gattcctgat
       61 gttgtctttc ttttacttgg aaactgatca acataatgga aaaaaacaaa gaaaaaaaac
      121 tgatgactat cgacaaaaga gatagaagca gataattaga tgagcggaag tgaacacttc
      181 ccaataccaa gtaaatcacg ccccagctta ataaaataca aatacctcca aaatagtgtt
      241 cagttggata agtaaaaagc aagttgagaa atctcgtctg tttgtctata ctttttacta
      301 atagatagaa aataaatgcg ataagtagtg caaaaaaact tgctgttcca agatgtctct
      361 ttcctaaata atccatcaga tcaaaaacca gaaaaaagct gagtccagtg atagcaaaaa
      421 aaagattact gatcaaacag ctgataaaca ggtttttcgt agtaaaataa aaaagaaaca
      481 tcaataataa gccaactagg taaggaaagc tcactttagg gataggaagt aaaagtgtca
      541 ccaccatcaa agctatacaa ccaaagctgt tttttactag cagttgtttc gaaaagaata
      601 aatagatacc taaatctaaa atcaaagtaa aggtcaaata aagtgtcact gttgcgacca
      661 tcttattcct ccattgtttt gtttcaaaat gtacacatat ttttttaata ggagcctgca
      721 tatgaaaaag tattttatca tagaagataa ccagtttcac agcgaatgga tcatcagaga
      781 attggtcaat catcatatcg agaatatcgt gcagctcact tctatctctc aaatcaatca
      841 gttgatccag ttgggcacta ttgataatca cgatgttttc tttatcgata tcgacttgaa
      901 gcaatcactc acaggtattg atgtcgccga acaaatacgt aaaaaaaatg cacatagtaa
      961 tattgttttt ttcaccgctt atacgaattt tgcagtagag atcatcaatc ggaaaatcct
     1021 tccgattgga tatatcagaa aagacaggaa tttatctgaa caaattcgtc aagtgctaga
     1081 acagatcaat tggaaagaac agcagatcgt aaatgtcgat aaaatcttta ttgatcaaaa
     1141 aggtggaagt ctcgtcctgt atcctcggga aattttatat attgaaagta tcaaaggttt
     1201 gaaaaaccaa gttatggtca aaacaactac agaagataaa ttagtgaata cttctttaaa
     1261 acaattgaag acagagttag aaaagtatgc ttacttttta cttttaaaat cttactgtat
     1321 caacttatcg caattgcaga aaattgattc tgctcattat gtgttagaat ttttcaacgg
     1381 tgactcctta ctcgttggtc gaaagatctt tgaaaagaca aaggaacgtt tccatgactt
     1441 ccaaaaaact gcttcgagtt gatgaataag agctgagacg taagcatagc agatttcagg
     1501 gagtgggcct tgaatcggct ccactccctg aaatcgttga tatgatttta cgcttcctct
     1561 tctgtaggag catctgttaa cgaccatgtg aatgttgaaa cgtagttgtc tacagcataa
     1621 ttggatacag gaacaaataa ttccactgat tgtctccctt gttccacatt ctctcctaat
     1681 gaataaatcc atgtgccaat cccttcattt gctcctgcag ttactagatc tactgcctga
     1741 tctgctggga tttcgattcc ttcagcgcca gttccagaaa cggtagatgg cattccactg
     1801 ctattagttg cgctatttcg aatcacggcg tttttgaatt tcacctttga accagttagt
     1861 ttattcgtat cagatgttgt accaataaat tctgttcgtt tgactgataa tttccaaccg
     1921 gcataatttc cgcttttatc cgttacttgt acgaaattct gtacttgtgt ttgtgctcca
     1981 gtactgtccg ttccgctttg taatgcagca tatactgttt ttgctgtacc taacttgatt
     2041 gttccaaaat caagatctgg agcttggtca atcgtcagta atccggatgt tccctgattt
     2101 ggttcgtcag gtgtaaaagg agtatcagga tcatttggat caacaggagg aactggaaca
     2161 acgtcttcag gatttgcagc atctggtttg gtcacagaaa tcgttccttc tgtagtggct
     2221 gagctcttat tctcagcaaa aacactttgt gtacataata atagacttcc agctagaacg
     2281 gtacttaatg caattgtttt tttcatttct ttttccttct ttcttttaaa ataaataatc
     2341 cagcaaataa gccaataaga atgactccaa caagtatcag ccatcgccag tcaaatgttt
     2401 ctggctgatt ttttaaatca atatcctttt cattcaattg gtccgctgca tcatcagtta
     2461 tttcaaaatc ggtatggaga ttccagcgtt tttctttcca agcacccttc acaaccgctt
     2521 gataatgtcc cggagctaat ttttttcctt ctaatgggac aagtaaatca aaatttgtat
     2581 tgggtgctac atctattttt tcttttttta ttgaaaggta aggctctttt gtttcttttt
     2641 tgtagatttc aaccgatgta gtcaagccga acatataaga agcatttgga ttttgaagat
     2701 tcgccttaat cacatttctt ccatcgcttt gatccgcata agcttttttt aatttcaaat
     2761 ctggggaaga ctctttttcg ctatttcaaa tcaagattgc tttcacttga gaaaactgat
     2821 tgacgacacc aactgtactt ttgttagaaa cgttctcttt tttttcttga aacgtgatcc
     2881 ctcctgcgac cacaccagcc agctcttttt ctggcgcttt cagtgaaagt gtctcagtga
     2941 ccgcactttg aggaggaaca gttatttctc ccactgtttt tgctaagttt ttcatatcat
     3001 aacttaatgt ttcatcaaat ttactttttg attctccata ttcgatcaca ccgtttgaat
     3061 tcgtcgtagc ggagtggatc aatgtctcaa ctgttactga tttatctgtt cggttaaaga
     3121 ccgtgtaggg gatttcctgt gttttcccag gctccatcct tacatttata tatgttttgt
     3181 tcttatcaat ctgattagcc ggtaattcaa aatctaccgc aaaattgaat tcggaggcat
     3241 ggacaggtgt ctgccaaagg atgaatccaa ggagaaggat cataagtaaa gagcagttgt
     3301 ttcgatgact tttattccat attcgattca tcaatcatcc tccttgatct ttattctgtc
     3361 ggcgcatctg ctaatgtcca tgttatctct gctgagtaat taccagctat ctgattttct
     3421 ttggttaaga caagtttaaa cgcccgaccg ttatttaaat attctgaata atctgcttgc
     3481 acattcgttg aattttgtcc tctgacaatt tccagttctt cttcattcac taattgatac
     3541 tcctctcctt tcttgaaata taggacatcc tgcatagaaa atcctgcatc atttgtgaaa
     3601 ggctgagata attttatcgt taagatccac ggatttttcg tttctcgctg atcataaatc
     3661 cataattttt cctgtatatc catttggcca aatgcttctt gtgtcaaacc agttgctttg
     3721 atctgaccaa aatcgagttt ggaaggggca agaaatgaga gaccaccttc tactacgatc
     3781 ttcgtagcag gtgcaaaagg cggggcatca tgataaacaa ctggcgtatc acttggattg
     3841 tgattcccaa tttcatcgtt ggtacttggc ttgttctgca cacccttagc agtagcttct
     3901 cctgcttggt cgtttaatac aatttctaat acatctcctg caaaaagttg attttctggg
     3961 atagaaagaa caaattcccc tgtatcatca atagttgttt ctgacaaaac agcttgattt
     4021 tttttcaatg aaaccaaggc tcctgcttca cctgtacctg tgatcgtgcg agaagctgca
     4081 ttgatcaatg tggtacttaa attagcaggt tcaggaggaa tcacatcgac tgtcgttttg
     4141 gcagagtcca gttctgtcac ttcttggcca cctcttactt ttttagcagc aacaacttgc
     4201 acttgatcct ccgtttttaa tctctcatca ttgggtactt gaacttcgaa caagccgcct
     4261 tgttcgcctt ctccccatgg gttatatcct gccccttcat tttcatctgt ttgtccttgc
     4321 gtgagagcag taatttctac agatgatttc gtcgtatctg cgtaagtaat acgtaaagtc
     4381 acttctactt cgcctgtcca agcatctctt ggctttgcct cgacaccttc gggaacaacg
     4441 acatgcccat agattttttt atctgcattc gttggcactc ttaaggattc aatcatcggg
     4501 agctgattat tggcactgat tcgattataa tttttcattc cggcacctgt ctgagcaaat
     4561 tcatttgtta aacctgcact agggcttgat acgaaattgc ctaagtcggt cccattgatc
     4621 gataagtctg tcatatcaga aaaataatcc gggtcttttt caaagttatc gaatgttgcg
     4681 cctgtttttc gccataaagc gatttctgaa tgggcgatct ttaaagaaga atctccattt
     4741 ccttggaata cccagccgcc tatgccattg gtctcgcttc caatccgttt gttttggaaa
     4801 tcaaaatagg taggagattc taagtccaca ttcaacacat ccccactgat gatgccggtc
     4861 ttatcttccc ctgttttccc gaccatgact aaataagttt tttcacctgc cgtaaaattt
     4921 aaatcaatat ctccagttgt atctacagtt gctccggaat ctgcgcgtat atcgatattt
     4981 gatttttctc cagtgactgt aaaatcagct gaacctggac tttctagaaa accagaagcg
     5041 tcataaaatt ggatcgcttg atttccttga ttgagtctgt tcacgtaaga tttgccatca
     5101 cctgtatttc ttaccctcaa tgagcctcca ttggttactg tcagcttttg cgtaccattc
     5161 tcaaaacgaa ctgcgctgga acgcccactc tgcttatcga tgtcgataac tgatccggct
     5221 ccttctacga tcaaagaaag gccttttcct acaaaacgca tcccattgtt cccgggttga
     5281 tcgtcatctc cagccatttt gaccgagatt tgtccaccac ttgatacatg gatttgacta
     5341 gaatctcctt gcaagcgaaa tccgcccgta gttgctgtat tgatggtgat tttcccacca
     5401 ttcgatacag tgactttcga tcctgaacct ctcatttgga tgattccgcg attgttgtca
     5461 ttggtaatat tgatctttgt atttaccgaa aattcgcttc ccgaaccact gacttcaaac
     5521 atgacattac caggtagcgt atcgactgcc tctccttcat cgctaacgcc atctgcccga
     5581 tcataatcaa gagagacctt tgaaccgctt aagatatgga ttcctggcgc tgtttttcca
     5641 ctaccagtct catctcctga atctagaaaa ccgtagaatt tccctagata cggtccataa
     5701 agttgactac cattgatcac attcaattga tagcttctac aaaaataggc tttacccgga
     5761 gcggcattca gtccttcctt cactggttgg acatccaaca caccggaaac accatctaat
     5821 gtgacagttg cacgaggagc gtaaatcagt cctaatttgg acccatcata caaatcaagt
     5881 gtcccggtaa attttatttc tcccgtaaaa tgatatctgc ttccccaaaa atcggtcata
     5941 taagcaatga taatggcatt tccatcgata tttggtgtca atccacctgg atgtgcagct
     6001 gttcctttaa aattctggaa ctcgatcgac tgaggtatct cattgatata aatatggtgt
     6061 gtgccgaaat tgtgagcgaa tccaccaaaa tcaataacga tatctcgttt gatctctaat
     6121 tccctgtcta aagtcaaagg ctgaatcagt cggatcttag ttactgattc gtctgcgaga
     6181 agctgcgcca gttcttctgt tgtggcagct tcgctgtacc catccgctga aacagctctt
     6241 gccagtgccg cttcactgct atcagacgtt tgttcagcta cttctgaggt ctcacttgtc
     6301 ttattttctt cttgcctttt attagtagaa gcctcacttt gagattctgt agtttcagaa
     6361 gtaatcgatt cttctgaaaa agtagtcttg tcaaaacttt gattctctga ttgcgttgat
     6421 tcgtttgttt tctctacatc ggcaaaaacg gatgtcacca tcaagagagg gaagacaaac
     6481 aaaatcgacg caatcagctt ttttacacat tgattcattc tccaccatcc tctctgacgt
     6541 tttgacaatt ttattttatc gttttaatct atttccaatt gtactttcat atttaaaata
     6601 tgtattttta tggaaaaatt tttctattag gtgtaaattt cttttttgta caaaaaaaag
     6661 gctgtgacaa aagtcttgtc acagtccctt atcccgaaca agcggtgttc tcccgccaac
     6721 tcctcggtca caacctccat tttaacaata aacctgtaaa tgtgagggca atactttgat
     6781 tgtcacaggt aatttatccc cttcatctcc atcaacattc gtcgtcaatt cttcctcatc
     6841 agctaatgac acaactaatt gctcacaagt aaggtaggct agatttttcg tagattcatc
     6901 tactcctttt aataggtctg gcaatgtttt aagcgtatcg atcaaagaag aatcttttaa
     6961 ataaaccaaa tggagcttac catcatccac ttttgcattt ggaagtaatg tttcaaaacc
     7021 gccgactgag ttagttagtc ctatcagcaa ggtactgctc tcaatttctt ccttctttcc
     7081 atcaacttct acgtgaaacg agtaggattg tgtggaagct aactgtttga ttcctgacat
     7141 gaaataagct agttttccaa atttcgtctt cttctccgca tctacatcat tgattgattc
     7201 agggatagag ccaattgcga cgacattcat aaaatagtcg tcatttattt ttccaatatc
     7261 caatggcttg actgattcaa tcgaaaaatg attgatcgct tcctcaggtt ctagcggtat
     7321 ccctaatgct cgagccaagt cattcaccgt acccaatggg aaaaatccga aatttggccg
     7381 atgttcttgt tcagcgattc cactgattcc ttcgttgact gttccatctc cgcccatgac
     7441 aaacacacta tgataccctt ccgtagccgc ttcgcgagta aagttttttg catcccctgc
     7501 tttttttgta tgcaagacga ccacttcatc aaacaccgat tccaattttg ctatcgctaa
     7561 ttgctcaaat tcctttgctt gttcaccgcc tgaactgggg tttaccacta ataaaacttt
     7621 tttcatcact ttctttccct tctgattgat ttctttaata taacgatagc ataaagaaaa
     7681 aaattaggct gtgatgaagt actgttcatg aaacattcat ttattttttg acaagataaa
     7741 acatccatac tatgaaactg aggaaaatta aaacaatgat ataggaaata aaagaaaatg
     7801 ataggcattg tatacctaat aaagtaaaaa tcttaggtgg cagataaaaa cacaaaaata
     7861 gcaagagaat gtaatagcgt ttcttccact ttttgttatc agaccttata gcgatcagag
     7921 gaataccaaa tgcataactg aaaaactctt gatgtactgt taatatgtca cggccttgaa
     7981 taataaatgc gacaatcccc gctataatta gtgtgcctaa caccaaaacg gaaaattgat
     8041 gaatatattt tatatacaaa tgatccattg tacttcctgc ctttttattt gtgagaaaaa
     8101 caaatccgta tacgtgaata acttctagaa ataaaagaaa tttgcaaaac cactccattt
     8161 atctacttct acgtgcattt ttt
//
LOCUS       DAIGOK010000111         8139 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.112, whole genome shotgun sequence.
ACCESSION   DAIGOK010000111 DAIGOK010000000
VERSION     DAIGOK010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.112"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            44..652
                     /locus_tag="NOA01_002179"
     CDS             44..652
                     /locus_tag="NOA01_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCI0633356.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            820..2457
                     /locus_tag="NOA01_002180"
     CDS             820..2457
                     /locus_tag="NOA01_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCI0633357.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELYLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(2529..3641)
                     /locus_tag="NOA01_002181"
     CDS             complement(2529..3641)
                     /locus_tag="NOA01_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCI0633358.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(3716..5089)
                     /locus_tag="NOA01_002182"
     CDS             complement(3716..5089)
                     /locus_tag="NOA01_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCI0633359.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            5310..5927
                     /locus_tag="NOA01_002183"
     CDS             5310..5927
                     /locus_tag="NOA01_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCI0633360.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6025..6480
                     /gene="argR"
                     /locus_tag="NOA01_002184"
     CDS             6025..6480
                     /gene="argR"
                     /locus_tag="NOA01_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCI0633361.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            6498..8000
                     /locus_tag="NOA01_002185"
     CDS             6498..8000
                     /locus_tag="NOA01_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HCI0633362.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
BASE COUNT         2792 a         1541 c         1692 g         2114 t
ORIGIN      
        1 tagcgtcgaa agaataaaaa acggagaaaa aaaggagttt aatatgaatt catttaagaa
       61 aattgtttta ggtacaactt ttgctgctgg tgctactgca atgtttgtag gaactacaaa
      121 tgctcacgct gatgaagttt atacagtaaa atcaggtgat tcattatcta agatctcaca
      181 aaaatttgca ggagacaact caatgatcga tgcaattgct gaaaaaaatt cgatcgcaaa
      241 catcaaccgc atttatgttg gtgaacaatt aacaatccca acttcaaatg attcttcagc
      301 aacaacagaa aacaaaacaa cagaaaatac agcatctaca actgaaacag caacgcaaga
      361 acatacatac gttgcacctg ttgaaacagt tgaagtagct cctgctgccc cagctgctgc
      421 aacagcgcca acttcaagct ctgcaaaaga atggatcgca caaaaagaat caggcggttc
      481 atacacagct actaacggac gttacatcgg ccgttatcaa ttagatgctt catacttaaa
      541 cggcgactat tcagctgcca atcaagaacg tgtagcagaa caatatgtaa catctcgtta
      601 tggttcatgg gatgctgcta aaacattctg gttagcaaac ggctggtact aagccgataa
      661 aaggaaaatg atgaaaccga gaaacttgtt tattcaagtt ctcggttttt ttgtgtgaaa
      721 aataactcgt ttgcttccca cagcgcagca gtacaaataa aaactcgaat aatgtataat
      781 aaagaaaacg tttttttgtt tagggaatga ggaggagcca tgagtacaaa agagagtctc
      841 gaattaaacg aaatcacaag tggaaatcca ctgttatcca gtattcgatc gattgtagaa
      901 acgacatttt acggtaataa tgttcatgaa gtttttgata ggaagacagc ttatcaacta
      961 gcaaaaggga gtccaggaac gatcatcaca gatttaacaa tctcacatgc tgaagaacta
     1021 tatttaccag ctgatgttag gacactagta ttcaatgatg gaagcattgt gggaagaaca
     1081 gcaagtgctc ggcggatctt tgaggactta gacaaggaac aaagcaaata cgagaaaatt
     1141 ttaagagaag ctgtgtatca aagtcgcaag cgccagtttt accatacaaa agtaatcgtt
     1201 ggcctatctg aagaattttc tgtgcaaagt catttgctga ttccagaagg atttgagaac
     1261 aatttgtatt cttatttgct gaattttcag ccagcgaatt cgaagatctt agagacttat
     1321 caaaagtctg tttcctttga tgagggagat ctttatatct tttgtgatcc cgaatggaca
     1381 catgaaacat ttccaaatgg actcattttg tttgatgctc tccataatac agcggctgtg
     1441 ctaggattac gttactttgg agaattaaaa aaagcgactt tgacgcttgc ttgggcattg
     1501 ggaaatcgca atggctttat tgcctgccac ggtgggatga aacaatatgc tagtaatgac
     1561 cagacctata cgatggctgt atttgggttg tccggttctg gaaaatcaac gattacctta
     1621 gcaaaacata cggaaggtca ctctgtttcg atcttgcatg acgatgcttt cgttatttct
     1681 cgaaaaaatg gttttgctac tgccttggaa ccttcttact ttgataaaac acaagactat
     1741 ccgtctgatg atccggccat ttcttctttc cttacttgtc agaatgtggg cgtgacatgt
     1801 acaagtaaag gagaaaaagt cctagtaaca ggagatatac gaaacggaaa tgggcggaca
     1861 gtcaaatcac gttatgcgac acccgaccga gtcgatcatc ttagcgaaaa gatcgatgct
     1921 gttttttgga tcatgaaaga tgattcgctt ccgccaatta tcaagatcaa tgacccagtt
     1981 ttagcagcgt cttttggtgc aacattagca acaaaacgat cagacgcgga aaatatcact
     2041 acaaaaagac agctagatga gctagtcatt gaaccatttg ctaatccttt ccgtgtctat
     2101 cctcttaggg aagactatca agcttttcga gagttgtttt tgaaacagca aacagcctgc
     2161 tatgtcctga atactggata tttcgatgat aaaaaaatca aacctgaaac gacattgtca
     2221 atcgttgaaa gaatcgtgca acaaacggca gaattcaagt cgtttggtcc atttgaagac
     2281 atccactatt tacctatacc agattttcct gtttcttttg aggatacgtc ctataaaaat
     2341 ctggtgaaac aacgacttga tattcgttta cgattcattg aagctcagat aggaaaagat
     2401 ctcttgccag atgaagcaaa agaaagtttg gagaatttga ttcagaaatt gaactaaaat
     2461 aacagctttt tcaagaagta atccatgcgc aatatcgctc gggatcctta cttgaaaaag
     2521 ctgtttttct atgatggatt tttcgtaata taggtggaga tgtccttttg ataatcttcc
     2581 gtaggccaat gaccagtatt taagaagttg gctaatagat agccgatata aggacctgtg
     2641 gtcaagccag aagagcctag accgctagca gccagtaagc ttggatcatc tggcagtacg
     2701 ccgaaaaatg gggaaaaatc tgacgtataa gcacgggtac ctacacggaa ctcgtatggc
     2761 cagtttttta tctgatctgg atcagtcaag aactgttctg tactcttagt caatccctta
     2821 taagcttcgc tagtaggagc caaatcccag ccttgatcgt tttcatgggt agcacctaaa
     2881 aggatctttc catccatgaa aggaatgaaa tcagcttctc cgtcaagcat agcaacaggc
     2941 cagtcttgac tcatcttata agatgtctga aatgctaaga gctgcccttt ttgcggacgt
     3001 atatctgcag catagcctaa ttcttgtaat aaaggcttaa gtgccgggcc aggtgtcaaa
     3061 attacacgat cagctgtgta ttctccaaac ggtcccttga tgagccaaga atcgctcttc
     3121 ttatgcaaag tgacttttgc ctggatctgc tccatttgtt tttgtgctaa acgctgacca
     3181 agatgttcta aatatgcttt tccatccaga cgaccaccac cactgatacg cagtgaaact
     3241 gctggtttca ataacggaag tgcttgagat gtctgttcag gtgaaagcca ttcgatgttt
     3301 ccaatttctg gcgcttcttc ttttcgtttc tcggcaagaa cagccaattc agctaaatct
     3361 ttttcaggtc gaagcaaaag tgtccctgat tgtgcgtaaa tcgtttgatc aagttggaaa
     3421 tcttttataa gtttagggaa aaaagcagcg ccgtctttag ccagacgata ccattgtttg
     3481 tttctgcgtt tcgacaacca aggggagata atcccagcgc tagctttagt tgcctgtcct
     3541 gtcccttcat caaataaagc aacattgaat tttgatgtat ctaagtaact ggctagggtc
     3601 ataccaatga tcccgccacc gataatggca attttttcca taaaaaagcc tccttttaca
     3661 aacaaccatt ttcacacaaa aaaacggcag atgcaacacc tgccgtatat ggaatctacg
     3721 cttttttagc aaagaacaga taataggcga atcgagcaaa gcttacgaga agcaacagca
     3781 acaagacgcc taatgctgct tttgatccta atgtagtaga cacttcatag ttatgggaag
     3841 tatgatgctg gacgtaattg ataatgaccg ctactaatga agtagccaca gcgccagaaa
     3901 actgctgcaa ggtattgaac attgcgttcc cgtctccttg tagttcttca ttcagtgcat
     3961 tcattccggt ggtcatcata ttgctgtaag ataatccgat accgatcata taaaacacat
     4021 gacaagcaat caatacaccc actgaagcgt gtcctatgag taggaataat gctagccaac
     4081 caatcaaaga aagaatcagc ccagcaaaaa ttggcttctt cggaccatat tgatccagca
     4141 ttttcccgga aaaaggagaa agcaaagcac caaccgctgc gccgggaagc atcgctaatc
     4201 cagcgacaaa aggactactt ccttgaacga tctgaaggaa attcggcaag acaaaagata
     4261 caccaagtaa taaaaactga taaactaaga agccaatcaa aaacaagcgg aatgttttat
     4321 ttgaaagaat agacagtcgg atcagtggat gctccacttt tttacttcga tagtaaaaaa
     4381 ggaaccagcc aataccgccg atgattaaag caatccagct ggaaactgtt cccaacgtac
     4441 tcaaaaatgt tagacttcca ctaaacaaaa gacaaattcc taaaacgctt atccaatcca
     4501 aataaccact tttttgtact gccatctcgg gaatagcaaa ccagccgata atcaaagaaa
     4561 gaaacaaaac gggcacaaga aacaggaaga tatgattcca tgtaaaatgt gaagtcataa
     4621 tgcctccata agtggggcca attgctggag caatggccgt tgtcaaagta ccgacaccca
     4681 tcatcgcgcc tcgtcgattg aggggggcaa atgtcaagat aatatggaac atcaacggaa
     4741 gagcgatacc tgtcgctgca ccttggagca atcgaccaaa caataggacc atgaaggtag
     4801 gagagaagaa atcgatcatt atacctgcca agaaaagcag atttgaagct aaaaagagtt
     4861 ttttgagact aaaattgcga ttcaaataag tagacagcgg aacaatgatc gaaatcatca
     4921 aaagataaat cgttgttacc cattggactt gtgcagtcgt taaatcaaac tgtctgataa
     4981 gagttgggaa agttacattc atggccgtct cgatcaatac gcccgcaaaa gacatcatcc
     5041 ctgttgcaat tactgcaggt actaaaagca gctgtttttg ttttgtcatt tacatcacct
     5101 ctaaaaaatt tcgtaaaaaa agagagtgat ttctattata aaaatagaaa ccgcctctct
     5161 ctcgacacgc cgaaacgtgt tatctgtacg atttatttac ttcacttatt ctagcacaaa
     5221 ctgaaaaata gtgtcaacac gattagccaa gtcactcaaa aagaggtatg ataataaata
     5281 actaaatgga atttcgcgga ggaagaaaaa tggaaaaaat tgaatggcgt aaagataaga
     5341 gctatagtat gaaaaaaagc ccgcaaatac tagaggttcc taaaaaatca ttctttagca
     5401 tcagagggat cggcgacccg aatcaggaag actttaaacg aagagtagcc tgtctatatg
     5461 cagtcagcta tgcaatacgc atgtcgccta aaaaaaattg gctgattcct gattatcaga
     5521 tgtatactgt ctatccactg gaaggacaat ggggactgca agaaaagtat ttgcatgaac
     5581 cagtgatgaa aaaagaacat ttttcttatc aattgatgat ccgacagcct gactttgtta
     5641 cagaagcgat agcacaagaa gccattcagc gttctagcaa acttccggaa gacttgaaag
     5701 aacaagtagt attcaaaaag atggaagaag gtctttgtgc ccaaatctta cacgtcgggc
     5761 cctatgccga agaaccagca acgtttgaga aattagaagc attcatcgca aaagaaggct
     5821 atgaaagagc ttctaaagaa cacaaagaga tttacatcag cgatccaaga aaatcagcac
     5881 ctgaaaaaat gaagacgatt ttgcgtgtta aaatagaaaa aggttagcaa cctgaatgaa
     5941 aacaaaaaat aaaagtaaaa aataccaaaa tatgaagaaa tattacttat cttttgctat
     6001 aatcaacatt gagaggtgtc taatatgaaa aagaaacagc gacaagcact gatccgccag
     6061 atcattacag aacaacctat tgggacacag gaagagctgc ttgcgcggct gcatgaagca
     6121 ggcgccgatg tgactcaagc aacgatttct cgagacattc gagaaatgaa gttagtaaaa
     6181 agtcaaaatg aaaacaaaat agtacgctat acattattta accagccatc tgtttcttta
     6241 aatgaagaac gtttgcgtac agcaattaga cgcgaggttt tgcgtatcca gtctgttcag
     6301 ttcatggtta tcgtattgac tgaacgaaat ggtgccgatg tggtaacgaa ttggctagat
     6361 gaagtggctt accctgaagt tgtcggaacg atggccggtg tagatacatt cattattatt
     6421 tgccgctcag aagaagaagc acagcgattt gcagaaaagc tggaaaaaat gagggaataa
     6481 atgaggagga cttttatatg aaagatatga aagcgctgca aaatggatcg gatattcgag
     6541 gaatcgcatt accagttgaa ggatatgaag taaacttgac gacagacatg gtaaaaaaga
     6601 tcggctgggg actagcaaac tggataaaga aaaagaagaa atcaaccgat ccaaataaaa
     6661 aatggacgat tgggattggt catgacagta ggataagcgg accagcactg aaagaggcgt
     6721 tgatccaaag tctgaaagaa caagggatac atgtcatcga tttcgaatta gctactacgc
     6781 ctgctatgtt tatgtccact caatttgaac agttctcgtg cgatgctgcg gttatgctga
     6841 ctgctagcca tcttccctat tattataatg gaataaaatt ctttacctca gaaggaggag
     6901 cagaaaaaga agatattgct tttatcctat cacagcagca aaaaataaaa agcgaacaaa
     6961 ctggaagcgt agaaaaagca gaccttttga caccttatgc gcaagattta gtcacaaaaa
     7021 ttaaaaaggg gattggaaca ctagaaagca aaccactaaa tggttggaaa atcattgttg
     7081 atgccggaaa tggtgctggt ggattctttg ctgaaaaagt attgaatagc ttaggtgcgg
     7141 attcaacagg ttcccaattt ttggaaccag atggccgatt tcctaatcat atcccaaatc
     7201 ctgataataa agaagcgatg gaaagtatca agcaggctgt tttagcaaat gaagcagatc
     7261 ttgggatcat tttcgataca gatgtggatc gttctgcagt tgtcagtgct tctggaacag
     7321 tgatcaatcg aaataatttg atcgcagtat taagtacaat cgtattaaaa gagcatccag
     7381 gaacaagaat cgtgacgaat tcccctactt cttctcatct acaaagattc attgaagaaa
     7441 aaggaggcat ccaagttcgt tatatttccg gctatcgaaa tgtaatcaat aaagcattag
     7501 aacttaaccg tcttgggaaa gatacacagc tagccatcga aacaagcgga cacgctgcat
     7561 tcaaggaaaa ctattttctg gatgatggtg catatgtcat cgcaaagatc ctgatgcttt
     7621 tgccagaatt aaaaagaaaa ggtatcacat tagatgaaca aatcaaagag ttgaaacagc
     7681 ccgtagaagt acaagaaatc cgattaaaat tgttcagcga tgaaccacgc gaacaaggaa
     7741 acaagatatt agaagaaatg aagcgactgt tggaagaaga aaaagggatg aacttcgacc
     7801 cggaaaatga agaaggtatt cgatatgatc tagaagagcc atacggaaac ggctggtttt
     7861 tgatccgttt aagcttacat gagccgttac tagtattaca gatagaaaat gatgaattcg
     7921 gaaagaacaa gttggcgtta gtaaaattag caaaattatt cggacatttt tctgatcttg
     7981 aatcattaac agcagaataa accagtcttt ttgaaaggct agttgcttgg acggcaaaaa
     8041 aagtggtatg gttaaatcga gtgctggtag ggagaccagc aaaaggagga aaaaatggta
     8101 acgaatatgg ttattttttt aacagtattg gtttatggg
//
LOCUS       DAIGOK010000112         7833 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.113, whole genome shotgun sequence.
ACCESSION   DAIGOK010000112 DAIGOK010000000
VERSION     DAIGOK010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.113"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(625..1971)
                     /gene="gor"
                     /locus_tag="NOA01_002186"
     CDS             complement(625..1971)
                     /gene="gor"
                     /locus_tag="NOA01_002186"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCI0633363.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2105..3358)
                     /locus_tag="NOA01_002187"
     CDS             complement(2105..3358)
                     /locus_tag="NOA01_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCI0633364.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(3364..4287)
                     /locus_tag="NOA01_002188"
     CDS             complement(3364..4287)
                     /locus_tag="NOA01_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCI0633365.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4508..7000)
                     /locus_tag="NOA01_002189"
     CDS             complement(4508..7000)
                     /locus_tag="NOA01_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCI0633366.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(7007..7474)
                     /locus_tag="NOA01_002190"
     CDS             complement(7007..7474)
                     /locus_tag="NOA01_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HCI0633367.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7610..7683)
                     /locus_tag="NOA01_002191"
     tRNA            complement(7610..7683)
                     /locus_tag="NOA01_002191"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7647..7649),aa:Arg,seq:acg)
     gene            complement(7741..7814)
                     /locus_tag="NOA01_002192"
     tRNA            complement(7741..7814)
                     /locus_tag="NOA01_002192"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7778..7780),aa:Pro,seq:tgg)
BASE COUNT         2181 a         1704 c         1260 g         2688 t
ORIGIN      
        1 tttccacatt tttgcataaa tttggcttaa taccgagaaa ctggcttttg aacaccgttt
       61 atcagaggtt gtaaaatcag tgttttgtcc tgagtattag agaaacaaat ggaaaaatag
      121 ttcctcatat tttgccgttt tttgagctaa taccgaagga cagctgattg aacactattt
      181 gttcggaaaa agtgactaac tatgacacaa tttttttcac agtccctttt ttcacttgat
      241 ttgattcgta ccagcgaact tgacgagaac tttcttggtt tggtaactat atggtattct
      301 tttaacatcc tcgtttgctt cttctaattt atttatacaa agtcacactt ttttaaacat
      361 ctgttcttcg atacatttct atttctttta aacatcgata ccaatatttt gaacttaaga
      421 gtgcatacca aatggattca tttactcttc gtcagttacg aagataatca aaatctccaa
      481 cttccctact tatttcgttt tgagaattat cgaagcaatc ttacataaat aactaaaaaa
      541 caaaaaacca gaaaatcagt atactgattt tctggtttta catggtttgt tcaataaaaa
      601 gctgttggaa ctgttaatat taaattatcg catcgttacg aattcttccg ctcctgttgg
      661 atggatggct actgtattgt caaagtcttc tttagttgca cccatcttga tagccacggc
      721 aaaaccttgc agcatctcat cgacaccaac accaatagca tgtaagccga cgattctttc
      781 ttcttttccg acacagatca atttcatttc gcatttttgg cgataatcat ttaatgcaaa
      841 gtacattggg gtaaagcgtg aacgataaac tttcaattca ttttcgccat atttttccaa
      901 tgcttcttct tctgtcaaac caattgttgc aattggtgga tgcgtaaaga cgactgtcgg
      961 aacaagttta tagtcaagat aagaatcttt ctttccatta aataaacgtt ctgataaacg
     1021 tctaccagct gcgattgcaa caggagtcag atctaattta ccgatcacat caccaacagc
     1081 aaaaatatgt tcatccgttg tattttggta tttatctact ttgataaacc cttttttgtc
     1141 caattccaca tttgtatttt ccagacctaa tttttcggta ttcggtacac gtccaccggc
     1201 aaaaagtaca caatcaccag ttaaggtttc accattttca aatagcaccg tatattcgtc
     1261 attgttttta gtaatttctt tagcaatatg atgtgcgtaa gtttttattc cagcttcttg
     1321 gtacatttgg atcaaattgt ccgaaagcat tttatcaaac gttctaagtg gtcgttcttt
     1381 tcgatatgcc cacatgactt cactacctaa accacgaaat acacctgaaa gttcagccgc
     1441 aatgtatcca gcacctaaaa cgattgctct cttaggtaac tctgttaatt caaaaaatcc
     1501 atttgaatca attgcgtatt ctccaccagg aatggtcatt ttacttgcac ggcctccagt
     1561 agcgatcaag attttaggag cacgataggt ttcaccgttt acttcgatcg tttgactatc
     1621 gataaaagta gcgtaacctt ttatagcctc gacatgattg ctatctaatc ctctttgata
     1681 agcaccgtgt agaaaatcaa tatatttttc tcgtctttct actagaccgg cgaaatcaac
     1741 attttttaaa tctgcttgga taccgtaact tgaggcatct cgttcaattg tttccaaaat
     1801 cgtacttgct tgccacataa cttttttagg tacacaaccg acgttgacac atgttccgcc
     1861 caattcattc gcttcaatca gcaaaacttt tgcgccatgc atccctgctc tgtttgctga
     1921 agcaataccg ccagagccgc caccaattac gatgtaatca tatatttgca ttttgttcct
     1981 ccttattgaa gagatcaatc aaaaccttta tttaacgatc ctttttctac agaaaacaag
     2041 tgtaacactt ttcttgaatt ttgtatgcga agatgcttaa aacagggata tatcagcttt
     2101 ttccttattt acgttttcgg atcatatctc caggtttcac cggctgtgcg actggcatag
     2161 ttaaaatcat cattggattt ggtgctcggt caatggattc gccttcttca ttatgcataa
     2221 cttcaacggt ttgactgaaa tgactgaatc ctggaccata aaattcgatt tcatctccta
     2281 cagaaaacaa gtttctctga cgtatcgtag caatctgtgt tgcctcatca taagataata
     2341 cttcaccgac aaatttatat actgggattt tacgacgttc accgaataat tgttcgtttt
     2401 cagttggtgt attgtagtaa aaacctgtag ctaattctcg ttgtgctact ttccataact
     2461 cgtcgatcca ttcttgcttg cagacatagt tttcaggatc ttccatataa ctgtctacag
     2521 ccgctttata gacatttgac accgtagata cataatggat cgatttcatt cttccttcga
     2581 tcttgaagct gtctacgccg ttttcaatca attctggaat atgttcgatc attgacatat
     2641 ccactgcact catcgagaat tcttcttcca cttcgcctgt atcagttaaa ctgtttcgtt
     2701 cttgaccaaa aggcatatcg aacaatccat acttccagcg acaagattga gaacatccgc
     2761 cacggtttgc atcccgcatc gacatatggt tggacaatgt gcatcgacca gaataagaga
     2821 tacacatggc accatggata aatgcttcga tttcggtatc cgtattttta cgaatctctg
     2881 ccacttcctc cattgaaact tcacgagcta aaacgacacg ttccaaccct tcttctttcc
     2941 agaattccaa tgtctcaaaa ttagttgctg aagcttgagt ggataaatgg atcggcagac
     3001 ctggggcttc tgtcgcacaa atttcgatca aagctggatc agaaacaatc actgcagaaa
     3061 taccgacatc acgcaaactg cggaaaaatt cccctgctcc atcttcattt ccttcatggg
     3121 tgaccatatt agcagcaacg tataccttcg catcatgttc ttttgcaaaa gcaactcctt
     3181 cagccatttc ttcataagta aaattacctg cacggcttct taaaccatat gcattcccgc
     3241 caatatatac tgcatctgct ccataatgga tcgcggtttt taacttttcc agtgttcctg
     3301 ctggcgctag gatttctggt cgtttcaatt ttctttttac tgtcatttct ttcatcatac
     3361 tccttatttg atagcagaag gatcaatata aaagaatcct gtatctaatt cacggttttc
     3421 aggatgcaat ttactgattt cgctagtcat tttttccgct ttttcttgat cccagctacc
     3481 ttctaaaatt tgtttttttg cttcggcgaa tagtcgagtg atgtttacga atttttcgcc
     3541 tggagtaaat aatccgtcca atttccatgt acagtaatga tgctgataaa gttcattcaa
     3601 ttcattcgag agattcagat cattgctggc aaaaatatgt gtgccatgac tatcttcata
     3661 aatagaataa tgtgtctctt cttttttagg ttccgagata aaaagacctc tttcacgatc
     3721 tttttgttcg tcttgtttcg tatagttata gtaattctgt agcagtggac gtttggattg
     3781 atggatgcat gtagcaccat acacaagggt ttctacagga atatcgagtt tttcttccat
     3841 cgccttcatc tcttcaaaag gaacttcacg agcaagtact gctccaactg cgcctttttt
     3901 cgcccagaaa ttgatttgac gtgcgctcgt tactaatgtt tcagcatcat aaatatacgg
     3961 tatacgcaat tcaggatttt ttttcatcgt ataaataata cctgtatcac caactgtgat
     4021 cacatctacg tttatttctt ttaaaaaagc caaatattca ggtacatttt tcattttttc
     4081 aggatgaatg atcccattta cagcaatatt cgctttttta ccatacttgt gtgctaattc
     4141 taccaattgt cgctgttctt ctcgtgtaaa tgaagcaggc agacgcaatc caaattgctc
     4201 ttcaccgaaa tatagtgtat ctgcacctgc agcaagcaac tgttctgctt gttcaatagt
     4261 ttctactgtg gttatgagtt cgatcataat tttctcctcc tacaacttca taatagtagc
     4321 atagctctaa aaactttcca atacttcttc tatttaaagt cggatttttg aaaattattt
     4381 cacaaaaaat caaaataaag ttcgatatat tctgtccaaa tataaaaaga gattgtgaca
     4441 aaaatcttgt cacaacctct taatcaagtc ttactattat aaaaattcag ctacacaggc
     4501 caagtaacta tagggaacct actgtttcct ttttaggtcc gcgaacattc aatgtgatct
     4561 taccttttga agcacctact gttacgtggt cacctaaacg aatttctccg ccaagcaaag
     4621 cctcgctcaa tcgatcttca atttcttttt gaagtgcgcg acggattgga cgtgcaccat
     4681 attcaggatc aaatccagct ttcccgatca catcgattgc agatggtgtg attttcactt
     4741 gaatatcttg ttctttcaat cgtttgataa tcgctttgct catgatttgg acgattgtat
     4801 ggatttcatc tttactcaat gaatggaaga cgacagtctc atcgattcga ttcaagaatt
     4861 caggtcggaa agctttcttt aactcttcta agatacgttt ttgcatggct ttgtgatctt
     4921 tagtaacatc ttggacattg aatccgacat ttttttcttc tcgaatttgt gtcgctccga
     4981 tatttgaagt cataatcatg attgtatttc taaagtccac ttttctacct ttagaatctg
     5041 ttaaatgacc atcatccaat acttgcagaa gtaaattgaa aacgtctgga tgtgctttct
     5101 ccacttcatc taagagaatc actgaatatg gtttttgacg aattttttct gtcaactgtc
     5161 caccttcatc ataccctacg tatcctggag gagacccgat taatcggctt gtactgtatt
     5221 tttccataaa ttctgacata tcaacacgga tcagggcatc ttcacttcca aacatgactt
     5281 ctgaaagtgc tttggccaat tctgtttttc cgacaccagt tggtccaagg aacatgaatg
     5341 aaccaattgg acgatcagga tctttcaatc cgcttcgtgc acggcggatc gcacgtgaaa
     5401 ctgctttgac agcctcatct tgaccaacga cacgttcatg cagaagtcct tccagttcta
     5461 acaagcgttc gctttctttt ttctccaact gctgtagagg gacaccagtc cactccgata
     5521 caactgtagc aacatcttct tctgttacac gatcagaata accttgctcc tgttttgctt
     5581 caattgcaga aactttttgc agctttttac gtagtgcttt ttcttgtctg cgtaattgag
     5641 cagcattttc aaagtcttgt ttttggatcg ctgcttcctt ttcttgtact agttcatcga
     5701 tttctagttt gatcacagtt gattttgtta agtgatcggt ttgatccaaa cggactttcg
     5761 ctgctgattc atcgatcaaa tcaattgctt tatcgggtag ttgacgagaa gtgatataac
     5821 gaacagaaag attgactgct gctcgtacag cttcatctgt gatctccact ccatggtgct
     5881 cttcatatct tgtacgtaat ccttgaagaa tcacttctgc ttcttcagga gttggctcat
     5941 ctacttgaat gcgagcaaaa cgtcgctcta atgcagaatc tttttcgata tatttttgat
     6001 attcatctag ggtcgttgct ccgatcgttt gtaattctcc tcgagctaac gccggtttca
     6061 agatattcga agcatcaatt gctccttctg ctccacctgc accaattaag gtatgcaatt
     6121 catcaatgaa taagattact tgaccatcat tatagatttc gtcgatgacc tttttcattc
     6181 gatcttcaaa ttcaccgcga tatttcgtac cagcaaccaa agatcccata tcaagcatca
     6241 tcaggcgctt ttcttgcatg tcttctggca cttcacctaa aatgattttt tgagctaacc
     6301 cttcagcaat ggctgttttg cctacaccag gttcacccac cagtacaggg ttattctttg
     6361 tccgtcggct aagaatctgg ataagccgtt tcacttctct cgaacgacca acaactgggt
     6421 ccagcttgtt ttcttttgct aatttcgtca aatcacgtgc taatgaatct agcgtaggtg
     6481 ttccttgcgg tgtttgccgt ttatcttgta catttacatt tctacgacga ttcattcctt
     6541 ttccagcaga atcaccagct agccccattt tttgtttcag aacttgacgg gcttttgaaa
     6601 ggctgatacc taagttgatc atgattcgag tagccatgat actttcatct tttaataagc
     6661 tcatcaaaat atgttctgtt ccaacttttt gagaaccagt tttcttcact tcgctgctag
     6721 ctaaagcaaa aacttgtttc attcttggtg aatatggaag aaagactcct ttagggtaag
     6781 aacggatacc accataacca atcagatgtt caatttcttc ataaatttcc gtttcattca
     6841 aatccatttc acgtaatact tttccagcaa ttccattttg ctcgaccact aaggctagta
     6901 aaacatgttc tgaactaact gattgatgtt tcaaccgttt tgcttcttct tgtgcaattt
     6961 ctaatactgc ttgggctctt tcagtaaaaa gttcttccat ctaatctcac ttcccatctt
     7021 cataacttaa acgttccaga aatgctcgga gaatccgagc acgtgtatga tcttcatatt
     7081 cattgaaatt caatgtgttt tttgcaattg cactcaacat caagtttcct tctttttttg
     7141 taattatttg gtcttcataa agtttttgaa taatagcaaa agcatttttc tcactgattg
     7201 tatcatcaaa taactgattt atctgctcaa gtaattgttt tttatctgaa atccttactt
     7261 ttgcaattcg tatataacca ccaccaccac gtttgctttc tactagataa ccacgttgga
     7321 tggtaaaacg ggtattaatc acatagttga tttgtgaagg aacacagtta aagaggtctg
     7381 ccatttcaat ccttcgaatc tcgatcattt cactattttc cagaattttc tttatatatg
     7441 attcaattaa atccgaggta ttctgatgtg ccatcttatt cattcccttt gactaatatt
     7501 gaccttaatt ataacgaatt tgataataga aagaaagaag aacgcttttt gttaaataaa
     7561 tcctttagaa tcaacaaaaa aaaatacgga atatactatt ccgtatcgac gcgcccaaga
     7621 ggagtcgaac ccctaacctt ttgatccgta gtcaaacact ctatccagtt gagctatggg
     7681 cgcatattca atttaaaaaa attataaatc atttgaaaac aaaaagcaag ataaaaaaaa
     7741 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
     7801 tgagctactt cccgtttaaa aaataaaaaa gac
//
LOCUS       DAIGOK010000113         7823 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.114, whole genome shotgun sequence.
ACCESSION   DAIGOK010000113 DAIGOK010000000
VERSION     DAIGOK010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.114"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(12..2183)
                     /gene="copB"
                     /locus_tag="NOA01_002193"
     CDS             complement(12..2183)
                     /gene="copB"
                     /locus_tag="NOA01_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HCI0633368.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(2203..4389)
                     /locus_tag="NOA01_002194"
     CDS             complement(2203..4389)
                     /locus_tag="NOA01_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HCI0633369.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="NOA01_002195"
     CDS             complement(4389..4598)
                     /gene="copZ"
                     /locus_tag="NOA01_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HCI0633370.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(4611..5051)
                     /locus_tag="NOA01_002196"
     CDS             complement(4611..5051)
                     /locus_tag="NOA01_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HCI0633371.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(5125..5664)
                     /locus_tag="NOA01_002197"
     CDS             complement(5125..5664)
                     /locus_tag="NOA01_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HCI0633372.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(6279..7745)
                     /locus_tag="NOA01_002198"
     CDS             complement(6279..7745)
                     /locus_tag="NOA01_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCI0633373.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         2125 a         1725 c         1370 g         2603 t
ORIGIN      
        1 ttttttcatt attatttgat tttcaatgtc aaagcattta gagctacaac taccgtactt
       61 aacgacatca agacagcacc aactgctgga cttaatatga taccaattgg cgcaagaatt
      121 ccggctgcta gcggaattgc aagaatattg taacctgccc cccaccagag gttctggatc
      181 attttctttc gcgtttgctt ggctagatca agaaaatgca aaatatcttt tggatcgctg
      241 tttgtcaata cgacatccgc agaatcgatt gcgatatctg tccctgcacc gattgccatc
      301 ccgattgaag cacgggctaa acttggcgca tcattgatcc catcacccac catgattact
      361 tttttccctt ggtcaaggta tttctggaca acagcttctt tatcatctgg taataaaccg
      421 ccataatatt cttcgatccc taaataatct gctactgctt gggcagcgtt tttattgtca
      481 ccagtaagca tgacaggagt gatttttctt tctttcaatg ttcggataaa ttcttttgct
      541 tctgttttta ctacgtcacc taaagcaacg attgcagcta gatgattttc taaaatcagg
      601 aagctgatgg tatttccttg ttcttggtaa ggtttcaata atgcttgttc tacgtttaat
      661 ccgagacgct caacttcttt ttcattaact attttcactt gttgtttttt gactgttgcc
      721 tctagcccga ctcctgaaag attttttaga ttttcagctt gataaggctt gatttcatgt
      781 tctttcaaat aattcataat gccaacagcc aatggatgat tggcattcgc ttccaaggct
      841 cctagatatt gaaggatttc atttttattg aacgcatcat caagtacttc gacacccgtt
      901 acagtaaatt ttccttctgt tagtgttcct gttttgtcca gcatgatcac atctagatca
      961 ttggcttgct ccagtgcatt gcggtttttc aaaagtaatc cgtttttagc tgctattgat
     1021 gtggagcgag caacaactaa tgggatagct aaacctagag catgaggaca ggcgatgata
     1081 aagactgtta ccatacgttc taatgcatta ggaagatctg tcaagaacaa ccaggcgatg
     1141 aatgccagga tacccacaat taatgcgaca taaaacagcc attttgccac tttgtcagat
     1201 aatgactcta gcttggattt ttctccttgt gcttttcgca ccatttccat aaccttcgct
     1261 agataaccat tttcaccagt tccagttact tcgatttcga tcgttccatc cccattgata
     1321 gaaccgccaa tcactttgtc attggcttgt tttttgattc ctcttgattc gccagtcaca
     1381 gctgattcgt caacaattgt ttcacctttc aagattacac catctgttgg cattttatca
     1441 ccagaccgga cgatcaaacg atcaccttcg tttacttctt tcaatgaaac tgtctcttct
     1501 tttccatctt tcgtcaagcg tttcactgat tctggcagta attctgctag tttttgtaaa
     1561 gcattgctgg cattcgacac agcattcatt tcaatccagt gacctagtag cataataacg
     1621 atcaaagtag ccaattccca gaagaaatcc atgacgtgtg tatgtggatt gagcagattt
     1681 gcgataaacg aatagacact ataaatatat gcaactgtaa tccccatcgc gatcagtgtc
     1741 atcatagcag gacttttttg tttcaattcc atcttagctc cacttaaaaa tggctgaccg
     1801 ccatagataa atagtatagt tgctaagatc aatacgagcc aatcagaacc aggaaacgtt
     1861 acttgaaatg gaaattccat tcccatcatt ggtgaaaaaa gaatgattgg gatagctagg
     1921 ataagcgata gccaaaattt ttgtttgaaa ttccccatgt gcatggaatg atccatgccg
     1981 ccgtgttcgt gatccatgtg catcgaatgg tccatttgtt tgctgtttgc tatatctcca
     2041 tggttatgtc ccatgtgcat ggaatgatcc atctgactat ggtcatgttt catctccatc
     2101 tgtttttcat gagattctgt actttctttg tttactactt cttttttatg ctcatgtttt
     2161 ttctccatat cgttcccatt catttttact ccatcccttc tcttactttt tcatgtgtcg
     2221 atttaagctc agggagttca acaatacact gacggaacta aacgccatcg ctcctcctgc
     2281 aatgattgga ttcaaaaagc ctagtgcggc aaaaggaata ccaatcgtat tatagacaaa
     2341 agcccagaaa agattttgtt tgatttttct caatgtcaac ctagacaact cgattgtttg
     2401 tgcaatggat gccaaactgc tattcatcag cgtcacatcg gctgtttcca tcgcaatgtc
     2461 cgttccactt cccattgcca taccgacatc agctaatgct aaggccggtg catcattgat
     2521 tccatcgcct gccataccga cttttttgcc atctttctga agtttttcta cgtaggctgc
     2581 tttatcctct ggaagtactt cagcaaagat atgctttgga tcaataccaa cttgtttacc
     2641 aatcgtttcg gctgccagtt tgttgtctcc tgtcagcata aaaacatcta atcctttatt
     2701 ctggagctgc ttgattgctt gtttggcttc tagtttgatt tgatcagcca cggcaatcca
     2761 cccgatcact tgcttttcat cagctaaaaa catgactgtt tttccctgct gttctaattc
     2821 taacgcgtgt tcttggtatt catcaaatga taggttcaat tcaattaatc gtttacgtgt
     2881 acccgcaaaa tagcgtactc cattaatcgt accgctgatc ccagcacctg ggtgggctgt
     2941 aaaatcaatc atatcgtatg ttgctgcttc ttgcagtttt ccgtattcca cgattgcttt
     3001 acctagtgga tgttcagaag cttgttctaa agtataaaaa atagatagga cttcttttga
     3061 accgactaga tctgtaactt tgggctttcc ttctgtaatc gttccagttt tatccaaaac
     3121 aatgctgtct aagtgagcag ctgcttctaa tgcttctccg cctttaatta aaattccatt
     3181 tcgtgcacca acacccgttc ctaccataat agctgttggg gtcgctaaac ctaatgcaca
     3241 tggacacgca atgactaata cagagacact atggatcaat gcgagttgcc aatcaccagt
     3301 gatcaaacct gtagctatga gcgtgataaa cgcgatgact aatacgatcg gaacaaaaat
     3361 ccctgagatc cgatctgcga ttttttggat tggggctttg ctcccttgag catcttccac
     3421 catttgaatg atttgcgcta atactgtctg cttgccgact tgtgtgactt ccatgtgtag
     3481 cagaccattc gtattgattg tcccgccaaa aagcgtgtca tccggattct tttctacagg
     3541 aagattttca ccagtaagca tactttcatc tatcgcactg cttcctgaaa taattcgacc
     3601 atctgctgga atctgctcac caggacggat gactagttga tcaccaacga ctacttcttc
     3661 aatcgctaac gtttcttctt tcccgtttct tatcacttgg gcggtttttg tttgaagaga
     3721 catcaattgt ttgattgcat tacctgtttt cgtttttgct gtatgttcca aatatttgcc
     3781 gagcaaaatc agcgtgatga tcatactgct gctttcaaaa taaagatctt gggggtgtcc
     3841 acggaaaaat ccgttgtaaa tgctcaatgc aaaggcagcc gatgttccaa ttgctaccaa
     3901 tacatccata tttggtgctt tcgtcttgat tgcatggtac gcacctttat aaaaacgagc
     3961 accgacataa aattggacag gagcagacag cactaactgg acgattggaa aatgaaaaaa
     4021 gtgaacaatc gccgcatggc ttcctaacat catagcaatc attgacaaca tcaacggcaa
     4081 agtcaaaacc gtactcaata taagatcgaa tagcattttt ttcaaatacg cttgcttttc
     4141 ttctgcaatt ttttgtttgt gggcttcatc gtacaaaatt gcaccataac cgatgttttc
     4201 tacgctttgg attaattttt cagtggtcgt cccttcatat ttgacacttg ctttttcagt
     4261 ggccaagttg actgtggcat tgaccacacc aggttgtccg ttcagctctt tttcgatcct
     4321 agcagaacaa tttgcacaag tcatacctgt aatgacgaac gtatccattt tggtttctgc
     4381 tgttgccatc aaatcacctc tgctttataa cccaatccat tgattgcttg acaaatcttt
     4441 tcggattgaa cttttgtttc atcaaacttg accgtaccgt ttgctttttt caaatttact
     4501 ttcacttttt gcacgccgtc aagtgcgctt actgtttctt ctactcttgc tgcacaatga
     4561 ttgcagctca taccttcgat tgaaaattgt tgtttcattt tttcttcctc ctattgatgt
     4621 tgtttacatt cacattgacc tgggatacag ttacattcga tcgtatctac tggttgcttt
     4681 tgttccagtt gtttcaaaat ctcattgata tcttcttgag tcaaagtggc ttcttctact
     4741 agatccgcaa tcgtctctcc aatccttttt gcacagatat gggaaaaaag attttccgtc
     4801 gcactgcgaa ctgtttccat ctccgagaca gctggatgat aaatgaattt ttttccttct
     4861 tgttctgtcc agatcacttc tttttttacc aaacgtccca gcaaagtttt gatcgttgct
     4921 ggtttccaat ccatcgaatc agctaagatc tgcgtgattt cttgggctgt cgtctgtcca
     4981 agtgtccaaa taacacgcat gatctcccac tctgaatcac tgatctttac tggcgttttg
     5041 actttttcca tttatttcac acctttcgtt tacaaatgta atcattagct tatgaataca
     5101 gattacgctt gtaaacaaaa actgtcaatt atttttgctc tctattttca atttagttaa
     5161 aaattcttgt ttctctcgtc ttgttttaag gatgatcagt ttatttcttg cattagctac
     5221 ttggatcagc tgctgtattt tcggttcatt gttttcttca aaatgccaga taaaagataa
     5281 aaattctaag tattccctgt ttagtttctc tgaaaatgat tcaggcatat ctggccgaga
     5341 ttttttgttt atctttcttt ttagagaacg ccaaactact cggtagatgg cgagataacg
     5401 cggcacttta agccaaatga tcttatctgc ttctttaatc ctttcctcta atgtagaagt
     5461 ataatttcct tcaacaatcc atgacggatt agataccata aacgattgct gttcttgcaa
     5521 aaaccacttt tttgcctgaa tactgtagtc tgttgtatgc cataaaaggt ctagcggcag
     5581 tagcggaaag tcggtgattt ctttgatctt ccttgcgagg gtcgattttc ccgacccact
     5641 ttgtccaatc acgatgatct tcattcttct ccctcacttc tctttggaat ttggttcgat
     5701 cataccactt ataacgccgc aaaaaaacaa gggactgtga caaaagtcgt gtcaaagtcc
     5761 cttgtttcga ataaatggtg ttcaatcagc tgaccttcag cattaactca aaaaacggca
     5821 aaatatgagg agctattttt tcgtttgttc ctctaatact caggtcaaat cgctgatttt
     5881 acaacctctt ccttaaacgg tgttctcctg ccaacacctc ggtcacaagg cacaatagtc
     5941 aacaaacatg agaagttgtt ttttgaaatt gctccttgtg actcggagtt aaacggcagg
     6001 ctcactacct ctttcttaaa cggtgttcaa aagcttgcat ctgcggtaat aagcccagca
     6061 aaacgaaaaa tatgaggggc tatttttgtt tggctgttct tattacttga tgcaggacag
     6121 ctttttcaca acctctgatt cacggtgttc cagaagtagc ttctgcgaaa aacaagccga
     6181 aacttcacaa aaatttgaga agcaattttc gtaaattcct tcttatttct tgaagctgac
     6241 cacttctgtc acaacctctt gtaacaagta tttctatttt aaggctgaac cactgtttct
     6301 ggttcagtga tttctttgtt tttgtctcga cgagcaattg ctggcaagat catacctaat
     6361 aagatcaaga caattggtgt gatgatattc gtgaataatt ggaaccacca tgctttagga
     6421 tcagcagtat aatctacttt tggcaccatt cctaggatac atgcaaacgc agtgaaggca
     6481 aagcaccaag caccaaagat aaaaccgatt tttggatttt taacaaattt atattcagaa
     6541 ttgaatttct tataagcttt attcaacatc atatatgcca agaacaccca taggtaacgc
     6601 attggcatga caactgagtt caagttcgtc aaccatttta ctaattcatt catatctttg
     6661 ataccaaaga tcggcaacaa aataatgatt cctactagta tacctgtcag aagataacca
     6721 ttcaccaatg tacctttacg attacgttta cgtagccagt ttggtacaaa ttctggatcc
     6781 gcatctgcta acaagatttt tagtggtgca tcaattgaaa aggctaatgc ggagatttgt
     6841 ccaatcatgt ttgtcaatgc gtaaatgatc atcaatgagt taccgatacc atagtaatta
     6901 cctaaaattt gaaaagcact gtatgcccca tttgccatca agtctttagg aatattatcg
     6961 ctagcaaaca gcatacccat tgcgactgag cctaatacag cacaaatccc taccattgct
     7021 gctaagaaga tcatcccttt aggaaattcc tttgcaggat ttcttgtaga gttgacataa
     7081 ggggatattt tttccgctcc tccaactgca aatactagca tcgaaacggt tgtgaaataa
     7141 ctgaaatcaa atgttggaat atatgttttc acttgattca tatgtggtgt ggcaaattcc
     7201 atcgttggat tgatgaacgg tgccgttaca gctaaaagaa taaataagat cgacatgacg
     7261 aacatcgctg ttccggccag tccgccaatc actttcaaag tagaaagtcc ttttgttgat
     7321 aaatataaaa aggcaagaaa tataataagt gaaataactg ctaccatttg tactgacatc
     7381 gtatttacga tatttccatt tccttggact gcccaaccgc cagcaatcag gattgcttga
     7441 ggcttttgtg ccaaataagg aatatggacg acccagtaag tccaagcggc atagtatgcc
     7501 agtcgtttat tagatgtcgt attttcgacc catgaactga cgcctccttt actatccttg
     7561 aaggctgacc ctaattgtcc aacgatcaat gcgtatggaa taaaataaat ggccaaaatc
     7621 aatagccaag aggtgacaac tgatactcct tgctgagcat agttattaac tacattcccc
     7681 atgccccaaa ccatgttaaa tgcaattaac gcaacagtaa accagcgtaa ttgtttgttg
     7741 ttcattcgtt ctctccttta tgataaaaat ttgttatcca gatgtaatca tattcaattt
     7801 ttaacaaact tacaagacag tgt
//
LOCUS       DAIGOK010000114         7815 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.115, whole genome shotgun sequence.
ACCESSION   DAIGOK010000114 DAIGOK010000000
VERSION     DAIGOK010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.115"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            74..775
                     /locus_tag="NOA01_002199"
     CDS             74..775
                     /locus_tag="NOA01_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCI0633374.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1347..2363)
                     /gene="tsaD"
                     /locus_tag="NOA01_002200"
     CDS             complement(1347..2363)
                     /gene="tsaD"
                     /locus_tag="NOA01_002200"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCI0633375.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2360..2824)
                     /gene="rimI"
                     /locus_tag="NOA01_002201"
     CDS             complement(2360..2824)
                     /gene="rimI"
                     /locus_tag="NOA01_002201"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCI0633376.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2831..3373)
                     /gene="rimI"
                     /locus_tag="NOA01_002202"
     CDS             complement(2831..3373)
                     /gene="rimI"
                     /locus_tag="NOA01_002202"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCI0633377.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3357..4079)
                     /gene="tsaB"
                     /locus_tag="NOA01_002203"
     CDS             complement(3357..4079)
                     /gene="tsaB"
                     /locus_tag="NOA01_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCI0633378.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDKIIVPMFDARRKNVYAGAYRWKENRLEIVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFYEIKEILPSAMINTVPAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4270..5250)
                     /locus_tag="NOA01_002204"
     CDS             complement(4270..5250)
                     /locus_tag="NOA01_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCI0633379.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5274..6758)
                     /gene="galT"
                     /locus_tag="NOA01_002205"
     CDS             complement(5274..6758)
                     /gene="galT"
                     /locus_tag="NOA01_002205"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HCI0633380.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6770..7759)
                     /gene="galE"
                     /locus_tag="NOA01_002206"
     CDS             complement(6770..7759)
                     /gene="galE"
                     /locus_tag="NOA01_002206"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCI0633381.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
BASE COUNT         2176 a         1690 c         1389 g         2560 t
ORIGIN      
        1 tatgttaaaa ctttacaaat tctttaaaaa aagtgacaat aaatatagtg aacaataagg
       61 agaggacttt ttaatgcgtg ttaataaaat cgattttcac ttttcaaaac tcagatatca
      121 acctgatttt gaacatttct cggatgaaga gttccaacag atcaaggaac atactgttct
      181 tcgttcttat aaaaaaggac agatcctttt cgacgatggt gatcccagac aacggttgta
      241 tatcttgacc aaaggactcg ttcgtttaga acgttatgat gaaagtgcaa cttattacta
      301 ctatgactat gtgaatacaa ataacctttt tcctatgtca ggaatgtttg gagaagagac
      361 ctacacttat acagctcaag cgatgactga tattgaaaca ttctaccttc ctgtttccta
      421 ctatgaaaaa ttagcacgaa aaaatcccga acagttgatc tatatgatac aaaaaatttc
      481 taaagtagtt gaaatacatg aattgcgtat ccaaatcgga ttgacctcta gcgcctttga
      541 ccgggtaaaa caaaatttat tcattttgaa agaagaattg ggcgtaccaa caaaagatgg
      601 tcatatcacc attccttatc cgatcacctt aaaagaatta gcagtgaata gcggcacgac
      661 acgtgaaact gctggacaag taatcaaaca actacgtgat tccggactgt tagattatga
      721 caaaaagcgt tttactttct gtaagacagc tactactcca gctatgagag aataacgcga
      781 acaaaaataa gcagtgaccc tgatatttat cagggtcact gcttattttc ctccctaaaa
      841 cttctgaaag atccggcagt tttagatcac tgggcaagac tcaaagattt tctatgtgtg
      901 tttcagttat gtaatattca ctgtttgcaa aaaatactga tagattaact agaggaagcg
      961 tcttgtcctc atccgaccta gattattcga tattcctcga caataaacga ttaatcagtg
     1021 agggcaatca gaggaacgga taatgatgaa taagctgtaa aaaatagggc tttgacaaaa
     1081 gcgtgtcaca gcccttattt cgattaaacg gtgtgtacct gtcaactctt cggtcacaag
     1141 tcataatgtt cataaacatg aggagctgtt ttttgaaatt gttccttgtg aatcagagtt
     1201 aaacgacagg cttacgaact cttgattaaa cggcgttcaa aagcttgcat acgcggtaat
     1261 aagaacagcc aggtcaaaaa catgagaagc cgtttttcct gggctgttct tattacttga
     1321 tgcagggcag atttttcaca acctctttat tcagataaaa ccaagcttgg atcagcattc
     1381 aactgatagc tagcaaaatt tcttttttgc atttccacat gtgctgctgc tccaatcatt
     1441 gcagcattat ctccacaaag tcgaagaggg ggaatcacta gttctacctc agggagttta
     1501 gttgccaaag catcctgcaa tccttcacgc aacccttgat tggccgctac acctccagct
     1561 acaatcagtt gcttgactgg ataattttga caagctcgaa gtgttttagt aatcaataca
     1621 tcgatcacac ttgcttgaaa gctagctgct aaatcatttt tgtctagatc ttctccacgc
     1681 tgttgtgcat tatgcaccaa attgataaat gcacttttta atccgctaaa actgaaatca
     1741 taattatctt catggatcat cgctcgtgga aaatggtaat tatcttttcc ctgatgagct
     1801 agctgatcga tttcttttcc gcttgggtaa gaaagaccta atactcttcc aactttgtca
     1861 taagcttctc ctgctgcgtc atctcttgtc tccccaatga tttcatatga tccatcttct
     1921 tgcatataga ctaattctgt atgtcctccg ctaacaagaa gtgccatcaa tggaaactgg
     1981 aatggtttaa ccagacgggc tgcatagata tgccctgcca tatggtttac aggaataagc
     2041 ggcaattggt gtgcccaagc aaaagcctta gctgcagaaa taccaatcag caacgagccg
     2101 acaagtcctg gtccataagt gacagcgact gccgataaat cttctgccga cacaccagct
     2161 tctaccagtg catcttccaa gcataaggtg atttgttcta cgtgatggcg gctagctact
     2221 tcaggaacta cgccaccaaa acgtttatga ctgtttattt gagaagcaac gatgttagat
     2281 aagatctctg ttccatttct aacgaccgct acactcgttt catcacaact tgattcaatc
     2341 gccagaatca attcttcatt catttcatcg tctccgtttt taactttctg ctcataatga
     2401 tcgcatcttc cgtcggattg cgataatatg atttccgttt acctaatata cgaaactttt
     2461 ctgattcata aagttttctt gctgcttcat tagatagacg tacttctaaa aaaacatgca
     2521 gcgaatcttt tatttgttcc gtaatcagta attgtaacaa atgccgtgca tgcccttttc
     2581 cttgttctga aactgcaaca gcaatatttg tgatctcgac ttcatctatt actttcgagt
     2641 atccaataaa gccacagatc tggttatctt caactaaaag cagataatca gagtgaggct
     2701 gttggatgtc tgccaaaaac tgcattttcg tccaaggtga accatgtgga taagcatttt
     2761 cgctaatcat ccatacgcat tctgccaagt acgcttcatc aaaatctttt tttctgcaaa
     2821 tcaactaatc tcactcattt tctaaaagtt ttatcatatc caacgcatct tcttttgttt
     2881 cagtgtaata tctttttttg atcgttgttg cttgaaaacc taacttccga taaagtcttt
     2941 gtgcatcttg attactcata cgcacttcta atgacatcgt ctcacagcga ttcattatag
     3001 caaagttctc gatttcgtca ataagcagcg aaccgattcg tttacgctga tatgccggtc
     3061 gaacggcaat attcgtgata tggcaatcta aaccgaacgc tcttgaaccg ataaaaccaa
     3121 tcattcttcc attatcaaag atacctagat atagatgagg aagcggtgac ttcaattctg
     3181 atataaaggc actttttgtc catggcaatt cgccattata gacttctcgt tcgacatcca
     3241 gcagctcttt gatatcttct gtagtcaatt ctcttagtac gtaacgacga tcgatttgtt
     3301 cgattgtttt ttctgtataa atcattttat cttgaaaaaa gcttctggca tacgctctaa
     3361 attttttcaa cataactttc gtctccaggt gtgtgcgtct ccaaccattt ttcttctgct
     3421 tctacccgtt ttaaatagcg aggaagaaaa gcttgtttct ctgcaactgg ttcagctaaa
     3481 gcacctaatt gagccacagt tacaccactt ggaatatccc atgctggaac tgtattaatc
     3541 atcgcactag gcaatatttc ctttatttca tagaagaatt tgtgacaatc actcccgacg
     3601 aaataaatag cttcattcag cttttgtagt tgtgtgaaca aatcagaagc agaaatatgt
     3661 acatcttcaa gtacaatttc taatctattt tctttccagc ggtatgcccc agcgtataca
     3721 ttttttcttc gagcatcaaa catgggcaca ataattttat ccacaccgat gcaattagcc
     3781 gcgatggttt tcagactaga tactcctact agctctttat ctaatgtata agcaatcgtt
     3841 tttgctgtcg ttacacctat acgaagccct gtataagaac cgggaccatc agaaaccgcg
     3901 attcggtcaa tgtcttttgg acttatttga agatctgcaa acaattggtc gattgcaggc
     3961 atcagtgtta cactgtggtt gcgtttgata tttgtttgta tttgtccaag aatttgttcg
     4021 tcttctacca caccgatcgc caaagtttgg tttgcagtat ctattcctaa tgtaatcata
     4081 tatttgatcc tttctgctat gacattctcg ccttccattg tagcatactt ttttattctg
     4141 aggatgaaaa taccaaaggc aaaactagta caatgataca tgattatctg ctggcgggca
     4201 aattttgccc cttcctgaaa cacaaaaaag ctgcggtatc actccatttt cccacagctt
     4261 ttccatcttt tatttcgttg attctcgaat aatcaactct gtttccacaa taatctttct
     4321 tgaaaccggt gctttggaat tacttaattg aagaatcgtc tccactgcta gctctcccat
     4381 ccattcggta taaactttta cagtagaaag cgagggcgtt acatatttcg ctacactgac
     4441 atcattgaag cccatcacag aaatatcttc tggtaccact aaaccttttt cttgaatcgc
     4501 tttcagtgcg ccaatcgcta atgcatcatt agctgcaaaa agcgcatcag gaacatgcgg
     4561 attcgttttt aaatactgct tcactgcttg atatcctgac tcgacagaaa aatctgcttc
     4621 gataattgga ccttcgtcgt agcctttttc tgccaaaact tgttgaaatg ccataattct
     4681 gggatctttt agccgctgat ggtttttctt cgtatattct gttccagcaa gcatagcgat
     4741 atgttgcgct ttatgggaga caaagtgttc aatgactttt tgaacacttg acttaaagtc
     4801 gacaactaga gagtcgattc cttgagaagt gccgtcagaa tctaccaaaa gcaaggcatt
     4861 ttgggatcct ttcatttcta ataactgagc ttcgtcgaat tttcctagtg cgatcgttcc
     4921 atctgccgga agatcactaa tggaatccca cgtttctttc aataaagtaa cgtttgcttc
     4981 ttctgccttt ttttcgattc ctaggcgaat agataaataa taaagatctt caagctcttc
     5041 cgtatcgtcg taccactgga tcaggcgaag cgtttgctta gtatttgctg catttttacg
     5101 atgtttcgta taatttaagc gttctgctgc ttcaaaaatt ttttgctttg tggctaagcc
     5161 aacggaaaga tcaggatcat agttgagaac tcttgaaaca gtagcaggag aaactcctgc
     5221 caactgcgca atgtctttaa ttgttgccat ttgtctctcc tcctttttta cgtttataaa
     5281 atcgcagtaa atcgttcaaa cgctgcctgt cctttttcat ctcgcttgaa tactccagcg
     5341 tcttccagta cacgtgcaaa aacctgacct acagattttt gtacgacatc ggtgaccgta
     5401 tctttcgtca aatcaggatg ttcttttttt aattgttctg cccacggacg atgatattca
     5461 gccatctggt tttcttctcc taataagaaa tgctctactt ctactagttc tggtttcaaa
     5521 cgaggtggta aaacagcaag ccccattact tcgatcaacc caatgttctc ctttttgata
     5581 tgttggacat ctttatgtgg atgaaaaatc ccatcaggat gttcttctga aacattgttg
     5641 tctcgaagga caaggtccaa ctcaaagtaa ttgtcccttc ttcgtgcaat tggcgtaatc
     5701 gtatggtgtg gtgtaccatc tgctgaaaaa gctctgatag atactgtttc atccgaatat
     5761 gctttccatt tttccaatac tgagttagct gcttcaataa gcaagttttt ctctgttgat
     5821 tgcaaacgga tcacagacat cggccatttc acgactcctg cgatgacttg cggataatct
     5881 tctagtttga taagtttttc tatcggtgct tttgccatag gaaattcatg acgtcctgcc
     5941 tgataatgat catgagaaag aatcgatcct ccaacaattg gtaagtcagc attcgaacca
     6001 acaaagtaat gaggcaagac ttcagtaatc cgaagcagtc tagcaaatgt ttcctttgtg
     6061 attttcatcg ggcggtgttg ttctgataaa atgatcgaat gttcattgta ataagcataa
     6121 ggggaatatt ggaatcccca gctttcaccg tctagattca ttcgaataat ccggtgattg
     6181 gtgcgggctg gatagttcaa gcgacctcta tatccttcgt tttccatgca aagcatgcag
     6241 gtaggatagt ctacttttac cgcctcgcgt tctgctgcga tttgttttgg atctttttct
     6301 ggcttagata aattgattgt gatttccagt tcaccatact ccgactctgc cggaaatttg
     6361 atgttttttg cgatggctct tgttttgatg tagtcatttt tcttacttaa attaaagaaa
     6421 tactcagttg cttcctctgg gcttttcgaa tagtgctgtg caaagaatgc attgacgact
     6481 gaaggtggcg gtgtgagaaa atccattaat tgcgcttcaa aaatctcttg ttctgctggc
     6541 aattctgaaa gaacaccatt ttctcttgcc ttagcgacaa gctgatccaa taagtctaca
     6601 gattcttcct taactggtct tacttcaatg ctttctagag catcttctcc taccatccct
     6661 aaaagacgat tctgtaaata taagcggtcc atttccatcc atccgccagc ttcgatcgca
     6721 agtgtggcaa aatcacaaat cgtttgactg attgacatat ctcattcccc tattcttcat
     6781 aaccatttgg atggctgaca tgccaatccc atgctgtttt gataatcgct ttgatatctg
     6841 tgtattcagg tttccagccc agaatctctc tcgctttttc acttgaagcg actaatcggc
     6901 taggatctcc agctcttctt ggtgctattt ctgctggaat ttcttttcct gtcacttctc
     6961 tagctgcttc tagcatttcc ttgactgagt atcctttgct gctgcctaaa ttgaagaagt
     7021 tgctttcatt tccttctttc aagtattcca aagctaaaat atgtgcagca atcaaatctt
     7081 ctacttgaac ataatctctg atacatgttc catctggtgt atcataatcg tctccataaa
     7141 cagccaaggc ttttctttga ccaagagcta cttgtaaaat gattgggaca agatgcgttt
     7201 ctggtgtatg atcttctccg atagatgcat ctgcttttgc tccggctaca ttgaagtagc
     7261 gcaaagcgac atagcgcatg ccatatgctt ggtcacacca cttcatcatt ttttccatca
     7321 tcaatttact ttcaccatac ggatttttag gattagctgg tgtatcttct gtgattggag
     7381 actctttcgg ctctccataa gttgctgcag tagaagagaa tacgatttta ttcacgttga
     7441 actcgtgcat gacttcaagt aagatttgca taccgtatac gttgttgttg aaatacatca
     7501 atggtttttc gacagattct cctactaaag agcttgcagc aaaatggatt actccttcaa
     7561 tcggttcctt ttcaaatacg gaacgtagaa attctttatc ccggatgtct ccttcataaa
     7621 aatgagcatc tggatgtact gcttgtttgt gacctgtgag caaattatct accacagcta
     7681 cttgataccc cttttgaact agctggtcta cagcgtgcga accgatataa ccggctccgc
     7741 ctagtactaa aattgtcata aaaaacactc ctttgattag taaaattgtg tttgttgatt
     7801 ctatataatt taaaa
//
LOCUS       DAIGOK010000115         7792 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.116, whole genome shotgun sequence.
ACCESSION   DAIGOK010000115 DAIGOK010000000
VERSION     DAIGOK010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.116"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            636..1199
                     /locus_tag="NOA01_002207"
     CDS             636..1199
                     /locus_tag="NOA01_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCI0633382.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1478..1765)
                     /locus_tag="NOA01_002208"
     CDS             complement(1478..1765)
                     /locus_tag="NOA01_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCI0633383.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(1755..2084)
                     /locus_tag="NOA01_002209"
     CDS             complement(1755..2084)
                     /locus_tag="NOA01_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCI0633384.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2389..2718
                     /locus_tag="NOA01_002210"
     CDS             2389..2718
                     /locus_tag="NOA01_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCI0633385.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(3005..3490)
                     /gene="vanZ"
                     /locus_tag="NOA01_002211"
     CDS             complement(3005..3490)
                     /gene="vanZ"
                     /locus_tag="NOA01_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HCI0633386.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3643..4554)
                     /gene="vanY"
                     /locus_tag="NOA01_002212"
     CDS             complement(3643..4554)
                     /gene="vanY"
                     /locus_tag="NOA01_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HCI0633387.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            complement(4982..5590)
                     /locus_tag="NOA01_002213"
     CDS             complement(4982..5590)
                     /locus_tag="NOA01_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HCI0633388.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(5596..6627)
                     /gene="vanA"
                     /locus_tag="NOA01_002214"
     CDS             complement(5596..6627)
                     /gene="vanA"
                     /locus_tag="NOA01_002214"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HCI0633389.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(6620..7588)
                     /locus_tag="NOA01_002215"
     CDS             complement(6620..7588)
                     /locus_tag="NOA01_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HCI0633390.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
BASE COUNT         2377 a         1548 c         1297 g         2570 t
ORIGIN      
        1 cactctcctt gtatgcttta gcgggtatta caatttgagt gtaacataaa tggctttttt
       61 atttgtctcg cttaattata caaacggcga agttaaaact tttttagcag attttaaagt
      121 ttcgtcaaaa ctaatatatt ctggtagctg agcattcaaa tagctttcag acttcagaag
      181 aaaattacga gcatttttat agtccatctc taataaagtt attctttttt tatccaatac
      241 attcagctca attccttata atattctatt ccttagtatc tcaacattat ttactttata
      301 aaacgaataa attttgtagt tctaattcaa atattgcatg ttatatctaa tatgggagca
      361 ttttataatt ttcattgtga taaactcgtt attaacttta ggatacacta gttaggatta
      421 aattaggtat ctagaaattt acttaagatt tttttgttta tttcgtatac acactcgtat
      481 caaaaacaat cgattatgag acaaaatgaa atgacctctt ttattaaata aaagaggtca
      541 tattttgtat cataacttgt tgcattaact atgtatctat aactcccttt tatgatacaa
      601 tcaagccaga attaaagtac gaaaggaaga aaagcatgag tggatacatt tatggttatg
      661 cccgagtgag tacacgttca caagaactaa atcgtcaatt agacttactc aatgaacaaa
      721 attgtaatga aatcctaact gagaaaatga cgggaaccaa agcggttcgt ccccaactaa
      781 atcgtttaaa agataaaatt cgtccgggag atacggtaat tgtagaaagt ttttctcgat
      841 tgggtcgtag tacaaaggat ttgattgacc ttgttaccta ttttgaagaa caccatgtta
      901 agctggtgag cttgaaagaa aacttcgata ccagcacgcc tcacggacgc ttaatgatga
      961 ctgtcttcca agcatttagt cagttcgaac gtgatttaat tgttgaacgt accaaagaag
     1021 gtttgaaaag tgcacgtgcg agaggtcgta aaggtggccg tccacgagtg aaccagaaag
     1081 atgttgatcg agcagtaaaa ttgtataaaa gtcaggttta tagcgtaaaa gaaattacgg
     1141 aaatgactgg tattagcaag gcaacactct accgctactt aaaagacaat agggaataat
     1201 ctattttaat ctttaggaat ctataatatt tatgggagaa ttagagtgat ttttttatta
     1261 ttactaaaaa acaatataaa ataacgcatt ttaagccctc tttgtgcata aggatgaagc
     1321 ttttaatttg aaagaggctt aaaatgcgtt ttaatttttt ctatttttca taaatatata
     1381 ggttagtggt ttaaaattat agggtattac aactctcagc tgttaaaaat tatcttttaa
     1441 agtgtcgtct atcctctcaa gttgaaacat tgggttctta cctaatcttt tttaattgtt
     1501 cataaaaatt ttcatgcgtt ccacatagta agataggtat cactgcatta tctttaattt
     1561 catatgcaac gcgatatgtc gtacctgcat acttgaatcc aatagcccaa attcctgata
     1621 aatcacccgt tttttgttct cccgaatgag gacgaactgc tatctcatca taaattatcg
     1681 ttaaaaattg ttcttttaat gttttctctt ttaatttttt tagatacttt attacgtgtt
     1741 tagaaggtag aatctcataa gccaatttct gctgccaact cctcttttgt catcggttta
     1801 gaatttgcaa cagtatcttc agcaatagat ttaaatgcag aagttaacgc attttttcta
     1861 tttctaaatt cttttaagat ctcttgtttg ctataacctt ctgaaagaat atcagccaaa
     1921 atatcttcac taaaatcaaa aaattctttt ttaggctcta caaactcata aaggatacca
     1981 ttctctacaa gcttagcttc tacttcaaca ccattgggca catcgaattc tttagggacc
     2041 gttaacataa tagaatttcc ttgttttctt gtttttgtaa tcatattatt tcctccatta
     2101 ttttacattt cgtaattact tagtaattac attgtacgct atttttagta gaaatacaaa
     2161 ataataaaac tcctttagaa caattttcaa aggagtttta tttgattagg aaattatgta
     2221 gaataataat atgtttttaa cttggaatca aaaacgcact tgcaagtaag tttacttcaa
     2281 tacatgaaat tgcttataat agtgtattgg aattaaggta gattgacaaa agagtgatca
     2341 aataggtata cttcattcaa ataatcgttt gaatgtgagg tgagatgaat gaaaaaagtt
     2401 tgccaagtga ctgtcgtaca taaagaaaaa gtaaatggaa taaaaaataa attggatcaa
     2461 aaagattttt caaacttact tgtgttaggg aaatgtttca gcgatttttc tagaattaaa
     2521 attttctatg ctttagaaac ctataaggaa atgtgcgtct gtgatttagc agaaatactt
     2581 actgctagtg tcgctacaac gtcacaccat ttacgttttt taaaaaaaca tggaatggca
     2641 aaatcacgtc aagatggaaa agtagtttat tactctttag cgaatgaaga catcctttcc
     2701 gctattcgtg ttttttaaag ttatcagaaa atctatctat gaaatcttag tttgggggta
     2761 ccgtcaggaa aatgcggatt tacaacgtta aggaaattta ttaaagagac caattcctca
     2821 gcactaaaat tgataattgg gctttctttg ttactatagt gttaggtaag ctttttactt
     2881 tatcaccggt tttcccctca cttcacacct acgggcgagt ttccccacat cccgttttcc
     2941 atctaatcca attcattcaa tatctttatg tgtatgaaag atcagattgc ttgatttaga
     3001 catcttacac gtaatttatt cttaaatggg tacggtaaac gagcaataat acgagcaaaa
     3061 gtatacctac aaaaataata actctgtcta attttttttg attcatatgc ttattgctta
     3121 aaccatataa tttcagtcca agaaagcctc caacagtatt tgtaattaca tctgttatgt
     3181 ctgtcgctcc aatagcgaag ataaattgaa ttatttcaaa agtaagactt aaaaccagta
     3241 caaaagcaaa cttaggtaaa aatccgattt ctttaaaatt gacattcaaa agcaagccaa
     3301 atggaataaa gattataaca ttatctatca tctctctgaa attcccagta gcagtaaatg
     3361 gagtcaagtt aagacttctt tgatgataat taaatactga taaaatattg tattgtaatt
     3421 tgaataacac taaccagatt agtgtcacta aatataaagc tagcaatcct ctagataata
     3481 tttttcccaa tataatttcc tctaattctt ttgtttaagt tgtatcatta gtatagcaat
     3541 gtcctagtta acgtgggttt taaattaaaa agctaacgtt accccaaaag tgctagtaca
     3601 ataatgctgt tcattaattt cgttggtttc attccgccat ccttacctcc ttgaattagt
     3661 atgtgttgat ccgggaaaca ctgtcacaat tacaccgtct atattgtttc ctgatatctc
     3721 ataacgaaga ttagtcggca catgaatggt ggtattttta gtaacaggat aataaaagat
     3781 ctcatatttt tccccattta cactaacaga aatggttttt tcttctttta ggtaatccat
     3841 atattcctcg agaacgaaat tcttttcttt cataatcgca ctatgtggta aaccaacata
     3901 gcgaatatgc catagttcat attgaattcc tgttaactct gttttgtcct ctggataacg
     3961 taaaatgaac ccgtatttcc aagcattttc ttctatccac tttccttcag gggctcgttc
     4021 cattttcgtc aagcttgatc ctacatctag tgataaacct gaattatgct cactataacc
     4081 tgctggtaag gcatactcag cccccatttc ttggtaaagc acactttgct catcaaagtc
     4141 tcgatagcca ctattaataa taaaatgact aacgccaccc tttacagcat cattgaccat
     4201 ctctgaaaat ttttgtgcta tttcttttga catataaata ttactatcaa gcaacccgta
     4261 tccatttatt aattcgtcat gtttagataa attcacgata tctgacttca cactttcttg
     4321 gcgaacagga tatttactat tgattaatag cagatttcct tgataaacct gttcttctgt
     4381 aattgttttc tcttgggtat tttcagaagt cccactattt tctaaatgtt ctttgggatt
     4441 ttgatcataa ttttgaaatt cgactttttc ctgagaaaac agtgcttcat taacgtagtc
     4501 ataacctaag tatattaaga ataacaataa caataaaaaa aacaacttct tcattttcag
     4561 tctcctcctt aactatatag ttggaagtat gaaatatttc tagaatcttc cccttaaacg
     4621 gcacaatctc gtttgagatt gtgccgtttt gccccgtaaa ttacagtcaa ttatttcgcc
     4681 ctactggatt tactattatc accaatgtag atgaattttc ataagtgata cccatgctga
     4741 atttaacgag gctgaactgc ctgttgacaa tgggcggtgt gaaataccac ccctctatgc
     4801 gatcagtctt attatcaggc tatcttgccg cagcgcacgc agataatgac cgcaatatcg
     4861 ctctgcctta catcttcaat actatctact atatataaat tccctaagaa ccagttacat
     4921 acgtcgggtt tcctgccgaa agagttagct tatatagttt gtccgtgcaa cggttaaaag
     4981 tttatttaac ggggaaatca aaatagctat tggggtatgg ttcgtctctt aatacatagt
     5041 gccaccattc gaggctatat gcttcaaacc cactgttttc catgatggag cgcaaacgtc
     5101 tgcgattttg cgcttcattg catgatattc catttgccgc atgatgagag cgttcatcca
     5161 taaaatcaaa tcggctcccc attggtacaa gctcacccgt gtctaatcga taaagcgtaa
     5221 gatcaatggc actgccgcgg ctatggcttg attttgaagc cacgtatcct tttgaaatca
     5281 tctcagttcg gtcaatattg ggataataac tttcctttgt caggttattt tccggctgtg
     5341 cagcccattg cataaaacag tttacagcac gattaggacg gtaaccgtcc catagaagca
     5401 atccgtaccc ttgggtagca gccagttctt ttgccttcaa aagcgattca gccaactcgt
     5461 atgtccctac aatgcgattt acttcataac cgtcaaccgg ttttccggtg aaattatccc
     5521 aagtggcata tttagcgtcc caacgaacac cgtgtactat ttcatctaaa aaagtaaatc
     5581 ctatttccat gcttatcacc cctttaacgc taatacgatc aagcggtcaa tcagttcggg
     5641 aagtgcaata cctgcagcgg ccatcatacg gggataacga ctgtatgacg tgaaaccggg
     5701 cagagtattg acttcgttca gtacaatgcg gccgttatct tgtaaaaaca tatccacacg
     5761 ggctagacct ctacagccga gcgctttata tatttttttt gccgtttcct gtatccgtcc
     5821 tcgctcctct gctgaaaggt ctgcgggaac ggttataact gcgttttcag agcctttttc
     5881 cggctcgact tcctgatgaa tacgaaagat tccgtactgc agcctgattt ggtccacctc
     5941 gccaacagct aacgcggcac tgtttcccaa taccgcacaa ccgacctcac agcccgaaac
     6001 agcctgctca attaagattt tgctgtcata ttgtcttgcc gattcaattg cgtagtccaa
     6061 ttcgtccgcg ctattgactt ttttcacacc gaaggatgag cctgaacgcg ccggcttaac
     6121 aaaaacagga taggtaaacg tagctgccac cggcctatca tctttattaa taacccaaaa
     6181 ggcgggagta gctatcccag catttttcgc aacgatgtat gtcaacgatt tgtccataca
     6241 aattgctgag ctttgaatat cgcagcctac aaaagggata ccggacaatt caaacagacc
     6301 ttgtatggat ccatcttcac ctgacttgcc atgcaaagct gaaaatgcta catcaacatg
     6361 gttgatttca tattcatggt tctttttaac aagtaatccg tgcatttttt tatccggcga
     6421 gagtacagct gaatagcaat tgtcgttttc ccattccgcg caaggttttt cgcacatttt
     6481 ccatacacca gatttcgtaa ttccaatgta taacggctcg tatttttctt tattaatgtt
     6541 agcggctatc tctattgcag attttaccga tacgtcatgc tcctctgagc aacccccaaa
     6601 cagtattgca acttttattc tattcatgct cctgtctcct ttcaaaatcc aaacagtttt
     6661 taatggtttt ttcaacggta tcacgcaacg cttgctcggt ataataggcc gtatgcggtg
     6721 tgattatcac gttaggcatt ctttgaagtt taagtaaaaa ttgattatca attggttttt
     6781 gggtgcaatc agagtagaaa aactcttcct ctccttccaa tacatccaat gcggcaccgc
     6841 ccagtttccc gttttctaat gctttaacca actcataggt atctacaagt ggaccgcgcc
     6901 cagtattgat aagaaatgct ccttgcttca ttctctgtat ttgttcgtgg ctgataatat
     6961 agtgcgtatc cgtattgagc ggcacatgaa gcgtaacgat atcgctattt tgcagcaact
     7021 catcaaacgg tacatagttt acctctatac ttcggctgcg actataagcc aacactttac
     7081 atccaaatcc tcgcagccgc tcaataaccg ctttgcctat ctggcccgtt cccaccacac
     7141 caactgtcat gtcgctgagt accttgccac ggtcgctgtc caacctgaaa tcatgttttt
     7201 ccacagagcg cacaatcgat tttacgttgc gtactgccat aagaattagc atcatagtat
     7261 aatcggcaac gctatccggc gagtacgcca cattgtcgac agtgatgccc attctcttag
     7321 cagcagttgt atctatatga ttgcagccga tgcttcgggt agaaatatat ttcacaccgg
     7381 ctctcttcag cgcaagaaga atagaggcgg aaatctctga tttatgtccc acactgatac
     7441 attgattgaa aggcgcggat ttggcgttgg attccgacac gttggcgtta attatcgttg
     7501 ccataacgcc aaagcgaggc gaaagagcat ggaatgcatc tgcctcatcc tgctcacatc
     7561 cataaacagt aatgccgatg ttattcataa ttaagaccaa ccctttctgt gaaaggcacc
     7621 aaataaaacg gggatgccaa tggtaatctt gtcaaatctt tctctgaaga acgaaaacgg
     7681 ctcgttctta cggtattaag tctaccgtgt aagaacgagc cgtttcttaa tattaaattg
     7741 ttaatttcct aagaaaaagt aaagataacc ttgagatttt cctaaaaaat ta
//
LOCUS       DAIGOK010000116         7622 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.117, whole genome shotgun sequence.
ACCESSION   DAIGOK010000116 DAIGOK010000000
VERSION     DAIGOK010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7622
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.117"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(11..703)
                     /locus_tag="NOA01_002216"
     CDS             complement(11..703)
                     /locus_tag="NOA01_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCI0633391.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEIGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1140..1709)
                     /locus_tag="NOA01_002217"
     CDS             complement(1140..1709)
                     /locus_tag="NOA01_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCI0633392.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1783..2106)
                     /locus_tag="NOA01_002218"
     CDS             complement(1783..2106)
                     /locus_tag="NOA01_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633393.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2260..3072)
                     /locus_tag="NOA01_002219"
     CDS             complement(2260..3072)
                     /locus_tag="NOA01_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633394.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3426..4274
                     /locus_tag="NOA01_002220"
     CDS             3426..4274
                     /locus_tag="NOA01_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCI0633395.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4419..5360
                     /locus_tag="NOA01_002221"
     CDS             4419..5360
                     /locus_tag="NOA01_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HCI0633396.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKTFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5350..7521
                     /locus_tag="NOA01_002222"
                     /pseudo
     CDS             5350..7521
                     /locus_tag="NOA01_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
BASE COUNT         2271 a         1512 c         1369 g         2470 t
ORIGIN      
        1 gggggatttt ttatcgttgt ggaatattta aataccaacg cttgtcatga aaatcttgtg
       61 ctccaccctt agtatttcct tctggatcgt ttgtcgcacg catcatgacg tatactttct
      121 tattagggaa atcacgcata ttgaaagata catgatagcc aacatttcct aaagtattat
      181 aagcttgatt tacgtctggt ctataaatcc catcagctct tactctagct agttcttttc
      241 cagtattgta gtccataatg aaaatatact cgtatttata attagcaatg tgccatccag
      301 ctacatgcaa gtttgcgttt tcgatttctc cgaactgatc aatgtgggcg taatttgttc
      361 catctgtcag cgtagaattt gcagcacctg ctcgagttgg atcaattact ggtttatcct
      421 ccgaagtagt tggattatca tccgtaaaac catgagccaa atcataggct agtttttctt
      481 tacttacacc tatttcagaa agataaccgt aaggatctgt atgatcgccc caaatatttt
      541 gtgttaccca taaatgcgat ttgattcctg gttggttata aggagtgtct aatgttaatg
      601 gaataccata tttcattgct gaatctcttg ccaattcaac gtatacttta tagttctttt
      661 caaaagtttc tttatcatgt gtgtgttgta actcgatttg cacaggactt ttggctcacg
      721 aggttgtcct actctacatc ttgctgatag atatgctgaa aggaaaacac caacattatg
      781 ttttgctttt tcagctagaa cgagacaagc aggaatacct ttagaagata tcaaaggttt
      841 tttaggtcat aaagatgttt ccactacaca agtctatgcc catatttctc ccgaagtgaa
      901 aaaaagatca atgaatcaac tagaaaacta tatagaagag caaataaaaa agcactcaaa
      961 ttgagtgctt ttttaagctc actatcacta aaactatcac taattttatt tataagcgaa
     1021 catgtttcta tcactgattt tttaaatcta gcgaaaaacc cttatcttaa aggcgttcgt
     1081 tcaattcttt ggcaaggtct tcaaagcctg gcttgcctag aagtgcgaac atggttttct
     1141 tatagctcat gtagaaaaat tttcttgatg atgattttat ccatcaattg agattgactg
     1201 ttttgctctc ctatgatttc agctatgatt gtccgatttt gcggttttct tcttaactgg
     1261 tagttgattt tcccagtttc catcaactcg ctaagcatat ccacgactcc gccaaatccg
     1321 gtctttttcg taataaaacc tagtgctacc attcgttcat ctagattttt tgctgtaatc
     1381 gtcacaccag taatgagcga gtgctgtaat gtttgggcca gctcttccat cccttcagca
     1441 ttggatacga cggaagctgt tttgttaata ggttcttcgt ttatttcttt tggttctttc
     1501 ctctccactt tcactgtttg acgaaggaca tctagttctt gcaagagcgt ttcgtattgt
     1561 tcttgggtca gcatcacgcc agctactttt tcacgattaa atatatatac gccagttccc
     1621 gcgtctcgcg cttgagcaaa tacatccatc ggcgatcgtt tcacttctgt gatcgaagat
     1681 gtgggaactt ctaatttctt taattccaat ggatttcctc ctttactaat cgctttttat
     1741 tatagcacag actgacttgt tttgatcttt tttccaaagg ttttatcggt ttttgcctaa
     1801 ccagtaattg atatcggtaa gaagtctttc gataccaaat gaaggatggt acagatacgt
     1861 tttttgatct tcgttatggt ctagtggaaa aaaatgatcg atgatcacta gatctgcata
     1921 ccgaggcggc ttatttcgag tacatacatg tatagagatc cgttttcgat gtcttagcgt
     1981 tttgttcagt accccagcaa gtcttcttgc ttcttggtgt tcatagcgaa aaaaaaagac
     2041 gattttcact tcaggagggt ggacatctaa ataccctcgt tcctggagtg ttttcagtaa
     2101 aagcatcccc cactctactg cggggtgtgc aagtgcttgt tggatcgcta gccgaaattg
     2161 atatagttta tctttggaat tgggaaattt ttctaatgtt tcctgataat aacgaacttc
     2221 ttctatcagc cggtcatgga taccaattac gtaacgaagt cattcttggt aaagtacttt
     2281 tgtcagcttt gatttgcttt cgctctcaaa ttcgctattc agccaaaaca gcgaatcaag
     2341 cttttcagtc acaatcgtca atttccgata gtcttgtgtc tcttctatcc gtttccaccg
     2401 cttttcagta atctggttac agctcaagcg caaaattgct agataatttt tttcttttat
     2461 tggcaaaatc tgctcctctc taaatagcaa ttcaggaaac aattcaaaga atagtgttcg
     2521 actgtctcct aaaatctcga tttctgccgg atcctttctt tgcttcaata attggatgaa
     2581 catctcagaa aaggactgct ctccatcatc tgcaaaaaaa tgcggaaaat ctttcaaaaa
     2641 gcaaaacagc agatatctta cttttctttc tgaaccaaag atctgtgctt ttttataaga
     2701 aaaagaaagg tggtactcgc caaaccactt tctcaatcgc tcttgcgtag aacgatcgta
     2761 cttctgctcg taaggttcat tcaatagcag atttttcagt aaacaaaacg ggggtgattg
     2821 gatcagaaaa acaaacaaga gataaggttc gctatatcct tgatcataaa ctaactgaac
     2881 ggttttctta tgaagaatca gctgaatggg acagtcgctt tccgccaaac gtttcagatg
     2941 ccgatccact gttttaacat cgattttcag ttcttcagct aaatcagctt ttcgccagat
     3001 caaatctttc attagattga ttagtgaaaa taaattctgt gcattttttg ataatagttt
     3061 ttgccatctc atcgatcatt tctcacctga tttctaaaca tattgtttga tcaaaatggg
     3121 ttcactccta aaaagataaa aaactccctt tctatttatt taaatacgca aatcttcctc
     3181 attatttttt attttactca atttaatcgt attctcattt caaaacgaat gtactttaaa
     3241 aaaacataat aagatcactt tgcttgtagg atgtattccc ttccattaca atgaaataaa
     3301 tagcgatttt atgagaaggt gatgtgaaaa tggaagaaaa aatagaagaa tccaaacaaa
     3361 aaatcgatca gttgatataa gaattagaaa aagcggtcat cggcaaacgt tcaactatcc
     3421 aattgatgat cgtggcccta ttagcaggtg gtcatgtctt attcgaagat attcccggtg
     3481 taggtaaaac gttactggtc aaagcactag caaaagctgt acagggaacc ttcagcagag
     3541 tccaatgtac tcctgatctg ctgcctagtg atattcttgg tttttccgta tacaattcac
     3601 aatcaaaaga gtttgaattt cgtccgggac cgatctttac cacgatcttg ttagcagatg
     3661 agatcaatcg tacgactcct cgtactcagt cagcactttt agaagcaatg gccgagaatc
     3721 atgcaacgat cgacaacaat acataccctc tagacaatca tttttttgtc ttagctacgc
     3781 aaaatccaat cgagtacgaa ggaacatatc ctttgccgga agcacagctc gatcgttttt
     3841 tattccgact acaaatcggt tatccttctt ttgatgacga acttttgcta cttttggata
     3901 aattcgaaaa agtgttggat aacttaaatg aagtattgaa cagttatgaa atagaagaaa
     3961 taaaaacaaa tgtggcgaat gtctttgtca gtcccgaagt ggcatcttat gccttgcagt
     4021 tagtgtcagc aacaagaagt catgctgcca tccagcttgg catcagtcca cgaggaagcc
     4081 tttcctttat tcaggcagca aaagcttatg ctttaatcca tgggagaaat tacgtcacgc
     4141 ccaaagattt gcaggattta gttccttacg tgttttccca tcgcctgatt ttttacgatc
     4201 gttcattgaa ggaagacgaa aagaaacaaa tcattcatac tcttgttgca caaattccta
     4261 ttcctgtgag gtagattgag gtagacaatg gaaaaaatca aagattattt cattaatttt
     4321 gtgttatttc tggtatattt catcattgtg ctttttgctc ttgtattcaa caattcttta
     4381 ggctggttct tgttggcctt ttttactctt tatgtatcat ggagcttgat ttcattaagc
     4441 agctttctaa agaaactttc ggttacttgt acattgcagc cactcgttta tcgcaaaaaa
     4501 agtatgcagg ttattttttc catagcaaaa aagagaactt tttctttacc tctccctcgt
     4561 ttacaaatcg gcttcccagc aatctttgca gaaaaaaaca agagattttg attcttacaa
     4621 ataaaccgca agaagtccaa ttgctttggc agccaaaaga aagaggatgc catgaaacat
     4681 taactgtcat ttttacagcc tatgatagtc taggactttt cagaaaaagt gcaaaaagag
     4741 agatctcttt tcctgtgact gttctgcctg ctttttgtaa gattcaagca gaaaaattac
     4801 agagagttct ggaaaaaaaa cagcagctga acgcgtacca aagaggtctt gattttcggg
     4861 aacatcgcgc ttatcgtcca ggtgattctt tcaaccgaat cgattggaaa ttgtctgctc
     4921 atactcaaga gtggttatac cgtgaatacg attatcaaga agaagaatgt tctcctcttt
     4981 tttgcttttg gggttcgccc agtccaaaat ttgaacctct gctagacttg tatttttctt
     5041 tatggcattt gagaaaagaa aagcagccgg aacttctgat attaggcaac cgcgcctttc
     5101 atggcaaaga ccccggtcat acctattttg cttctttgga agctggcgat gagaaaactt
     5161 ttttcgcaca cttgaaaaaa tatgcaaaaa aacaaatcgt cctctttgtt cctgagaatt
     5221 ctcctgaagt ctcagaagct attgaaacat tgtcaaaaga tcgaacggtt tatcttgttt
     5281 atttcgtaga aaaacaactc atggtccaag aaaaagacaa gatatgttca ttgccaggag
     5341 gtgaatggat tgacgattaa acgggaaaaa tggctgcgtg gcgtttactc ctttgtgata
     5401 ctaagtgtag gtgctccgat atttgtagta gctaatcaat tgcaaaacag ctaccttctt
     5461 atcctaacta ttggtgtcct atgccttatt gtctgctgta ttcctaaaaa atgggtacgt
     5521 ttgctgcttt ctttgccagt aatgataggt tgtttgtttt tatattttcc ttcgaagaca
     5581 ttttccatat tatggatctt acagtttttt tcgtcagtta cagaggaatt cttccatcta
     5641 tcccaaggta aactttatta tttgccagaa aagacagctt tttttctcat aatgttgctg
     5701 atctatttat ttattacatt gaccgtggat tacgactatt ggtacgcacc ctttctttct
     5761 ttgatggctt acttcatgat gcttaccgtt tttcggaaaa aagatttact gtacgaaatg
     5821 attgcggtag taactgtttt gttcgtgtat ctggcagcaa ggcaattttt ctccatccgg
     5881 ttgttgtcga gaaacactcg attccagtca ttgactacag ttctgcttat ttgtttctta
     5941 acttttagcg ctatcttgcc tttatatctt ccgaaagtac accaatctct cgaggaaacg
     6001 agtgaaccga tccgagaata tcttaatgat gaagggcttt atgacacact ccatgagtat
     6061 caattaaccg gaagtgcaag aacaggcttc agtgagaatg atgaacagct tggtggacct
     6121 ttatatgata atggcgaagt tgtttttaca gcgaaccaaa aaggaaatgg ctattggaag
     6181 atcgaaaata aaaatcatta caccggttct ggatgaatag aagatcagga cgcacccatc
     6241 caaccaatcc aagaattccc tgttgacatc acgagctacc aaaccgttat agaagacaaa
     6301 gaaaacatta cattgaccct aaaaaaacaa gctttcttac cttatcctta tggaaatact
     6361 accttgactg atttttcatt agctagtgga caaacgcttt actatttgcc tgaaagccag
     6421 cgattcattc ctgtcccgaa tgaatcgaca cgttctgcaa ctttacaaac agaaaggtta
     6481 gtttttacga gagaagaatt aaaaagcagt cagttaaaag ctgatgattt agaaaatcag
     6541 cagacaaatc ttcaattgcc aaaaagtttg cctaaacgta ttgaggactt ggcggtcgaa
     6601 ttgaccaaaa atgaagcaac tttaattcgt aaagtcgaag ctgtctaaca gtatttaaaa
     6661 acaagcggaa atttccgcta ttccaaaacg gataccgggc atactccaaa agaacacgat
     6721 tatgtggatt atttcctctt tgattcaaaa gtcggctatt gtgacaactt ttcaacttct
     6781 atggtcgtcc ttcttcgaac acttggtatc ccagctcgct ggacaaaagg ttttacaaat
     6841 gggctcagaa caggcacaga taatcaaggg aatgaggaat acagcattct caatagtcat
     6901 gctcattctt ggccagaggt atactttcct ggatttggct ggcttccatt tgaaccaacc
     6961 ccttcatttg ccaatccaat gggttcacaa ccagttgctg aaacaaccga aaccgtccag
     7021 cctgctatcg aacaaccaaa tgaaagcgaa aatatggcag aaactatttc cagtgagaga
     7081 tctgaggtca ttccatcaga agaaacaaca ctcacaccag cttataaaat cgtcttattc
     7141 agccttggag tgtgtttatc cgtcttgttg ttctttctat tgttattcag aaaatctctg
     7201 cgctggacaa gttatcttat ctatcttcat ttttttgctt ctactaatac gattgcatat
     7261 caaaaacttc ttaacccttt ggaaacattg gtaccgcgca aacccgcaga atctttgcag
     7321 gaatatagcc tgcgaataga acaagaattc aagctagatc tgcgtttcat tcgattaact
     7381 actcaatatg aagcagaaat atatggacga caagcagatc cggtaaaatt cgataaaaaa
     7441 acctatttag aaataacgca tataataaga aaaacggtat acaaaaaaat tctgacaaaa
     7501 gggagccgct tcaaaaaata agataaaatt tctggagaat ctacttctcg aatgccgaga
     7561 accagctgat tgagtatcga ttactcgaaa aaaaggttgt gacacgtttt ttgtcacaac
     7621 ct
//
LOCUS       DAIGOK010000117         7536 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.118, whole genome shotgun sequence.
ACCESSION   DAIGOK010000117 DAIGOK010000000
VERSION     DAIGOK010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7536)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7536)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7536
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.118"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            12..923
                     /locus_tag="NOA01_002223"
     CDS             12..923
                     /locus_tag="NOA01_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633397.1"
                     /translation="MGERTFEDVKDYVELQSQEKCTVLSAKPEQEFNDLGTEVTVWNV
                     KTDTDGDWWVVEGDNVPMNLYPQSAYYFGTDEVYSFHMGLMERMRVLHEKYDPEDFVR
                     SVTLDTEIAPQLFRKLKNVATLIDSAEEIEDFQSIGVQCREILIELGNHIFIADMAGT
                     KEQPQKSNFKKKAELFIQFYLTGSDNSDYRSIIKKLTESTWDYANKLTHSSTTTKYEA
                     STCVMLVTSLVGVYENITQKVFDPVSQYICSSCKSKKLTIDSDDSDENGMVESLYLKC
                     EECGDITKVVFLTSDKDSSYTKGKIIE"
     gene            complement(2231..2896)
                     /locus_tag="NOA01_002224"
     CDS             complement(2231..2896)
                     /locus_tag="NOA01_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCI0633398.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            3076..5058
                     /locus_tag="NOA01_002225"
     CDS             3076..5058
                     /locus_tag="NOA01_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HCI0633399.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            5062..5700
                     /locus_tag="NOA01_002226"
     CDS             5062..5700
                     /locus_tag="NOA01_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0633400.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            5728..6156
                     /locus_tag="NOA01_002227"
     CDS             5728..6156
                     /locus_tag="NOA01_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633401.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            6613..7524
                     /locus_tag="NOA01_002228"
     CDS             6613..7524
                     /locus_tag="NOA01_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCI0633402.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         2598 a         1138 c         1435 g         2365 t
ORIGIN      
        1 ggaggtaaat aatgggtgaa cgaacttttg aagatgttaa agactatgta gaattgcaga
       61 gccaagaaaa gtgtacggtg ttaagtgcca agccggaaca ggaatttaat gatcttggta
      121 cagaagtaac ggtttggaat gtcaaaacgg atactgacgg cgattggtgg gttgttgagg
      181 gtgataacgt tcctatgaac ctttatccgc aaagtgcata ctattttgga acagacgagg
      241 tatattcgtt tcatatgggt ttaatggaga gaatgagagt tttacatgaa aaatatgatc
      301 cagaggactt tgtaaggagc gttactcttg atacagagat tgcaccacaa ctctttcgga
      361 agctaaagaa tgttgctact cttatagaca gtgcagaaga aattgaagat tttcaatcta
      421 ttggtgttca gtgtagagaa attctaatcg aacttggtaa tcacatcttt atagcagata
      481 tggcaggtac taaagaacaa ccacaaaaat caaacttcaa aaaaaaggcg gaacttttta
      541 tacagtttta tttgacaggt tccgataact ctgactatcg aagtattata aaaaaactaa
      601 ctgaatcaac atgggactac gctaataaat taactcattc tagtacaact actaagtatg
      661 aagcctcaac ttgcgtaatg cttgtaacgt ctttggttgg tgtgtatgaa aacataacgc
      721 aaaaagtctt tgatcctgtc tcacagtata tatgttcgtc gtgtaaaagt aaaaaactca
      781 cgatagattc tgatgattct gatgaaaacg gaatggttga atccttgtat ttaaaatgtg
      841 aagaatgtgg tgatattaca aaagtagtat ttttgactag tgataaggat agttcataca
      901 caaaaggaaa aataattgag taatgattat ggatcatgtc aaaaacatat cacgcaattc
      961 aaagtgcaca cgtttttaaa tttgatacct caaataactt ctctacctgc ttttttgggt
     1021 acgatattgg atacgttctg ggggatatcg agtgctgtga taggctgtca gtgatatata
     1081 ccagagcggg aaaaatgctt ttataacgcg aatcatgtac caatgctatt gaaaagagtc
     1141 attggaaggg tctgtggggt aatacttaaa taaaggctag gaacctgata acatcaaggt
     1201 tcctagcctt tttataataa aatgaagcga aaataatttc ttttaaagag ccgagattat
     1261 cagctatata agagtgaatt tgatgcaaaa aacagagccg aaacagtctt ggtgttcgta
     1321 gctctgctta tatgtttcga atttaagaca aagagttggc aaaagatgtt gataaggaag
     1381 ataagataac tgtgttatat aaactaggta actaactgta acaagctaat tttaagagat
     1441 tcttattaga agacgtttag gaatataact aagaaaatga ttatacaggt aattgtcatt
     1501 tcaataatgt tttctaattt tcgcgtcttt gttttaagaa aaaggccaga atcagttgga
     1561 aaaaagttcg tacgatagtg aagaggggca aaatttccta ctaatattcc cactaacaag
     1621 agataaaacg gccaggttgg tgtgattcct ctataaaaaa atgcccatgc taccgaaatc
     1681 ccatagatta acaactgaac aatttttaca atgaaaaaat tgttctgcca ttactcaaag
     1741 tctcttcctc cttgtatcgt ttatttgttt cattttatca ttatgatagg cgcttgaaca
     1801 atccattttg tattttattg agtactcttg gataaactac cagcgattat catatttatt
     1861 tatgttactg taagccatca tataaaagta aatgagcaga tcatagaaat ataaaaaaga
     1921 actaaaattg atctaaatta tgtgcagctt gactacgtac caatctatat tacttactag
     1981 attggtcgtt aatctattcg gatcgaatga gcaaaatatt tttgtgaatg tccatccttg
     2041 attatgattc caaaaagaat cattgggagg ctgtggcgta aatattatac tataagccag
     2101 aaatacctat cattctgaac ttttttattt aggaactttc atctcaaggc tttttcaatt
     2161 aatttgtacc cctgtttttt ggataagatc atgtgcattg attttttcta tctctacgtt
     2221 catctcctac ttaatgccta tctggctcct gagggcttct ttttaaggcg tcgtcatagt
     2281 cttggctgag gatttcttgg taatctgagg gaatggcttc ccaagtgagc aggcgaacgt
     2341 tcatgacgtg ttttcctttg tgtttgatta cagcatggtt tttctctttt tctacctctc
     2401 cagcgtgaaa agacagccat gtaccatggc caaagatatc attacatacc caacgttcga
     2461 tggaattgtc cacgtatcct acagggagtg tggtatagcc gatttgtggt ttcaaaaaag
     2521 gattgaatcg atcgatcgtt tcatttttgt attgataatt gataagaaaa agatagacca
     2581 aacaaaagac gagcagaaag aaagatagtg tctttttgag tgagaagggt tgttttgatg
     2641 aataagattt cttgttaaca agaggttcat ctggcaatgt tggttctatt gattcggtag
     2701 ctgattcaat agaaggaaaa tcaatcggca gacctaaagt atgatgtaaa agtgacagca
     2761 tttcaaggtc tggcattgta cgagaatttt cccaactaga aatcgtttta tcggaaacac
     2821 caattttttc gcctaattct ttttgcgtaa gcttacaatt tctgcggtaa gttcgtattt
     2881 tttctccggt gttcatcttc ttctcctttc gattctacag tatatagatt tattctacat
     2941 gacctataca cttgatagat acgttttttt gatgaaaaac ggtatgattt tatcaaagaa
     3001 tcatttaagt aagggagagt ggtgataatc aaaaaaaagt tcacagtttt actcacaatc
     3061 atagtgaccg gtatcatgag tggttttgcc ttggttagta taaataaaca ggtactctat
     3121 cggacaaatg aatttttcca tcaaacagat acttatactc cgattcgatt gccacaacat
     3181 tttattgggc aagaagaata tgagcagcta atcatggtat ttgaaaatgt ctcatcagaa
     3241 actgagcttt cttatatgaa aaaaacagta aatgaatcca atgaagtaga tacatcttgg
     3301 aacttcaaga tacagccaaa gcttgaaatg acattgtatt atcaagggga tgtcaaatct
     3361 tctcacgaga aggtacccta ttctaatacc tcctttcgcg taacgtccat tgaagaatcg
     3421 atcaaaataa aagattttga aggtgaattt ttcttgatga cgagagaaaa agaagcctat
     3481 gaacggttca tttcactatt cactgatcga ttcaatcaag catttcacac tgattatgcc
     3541 actaatattt ttgaagactt tgaaaatagc tattcgtatt cggttatttc aaatacagac
     3601 gtatataatt atcgagtgta tttaactatt ggattgattc ttttattcct tttgttatca
     3661 tcctttttct tattgatgaa taaagaaatc agtttattgt tgaataatga cttttcatta
     3721 catcaagtat tgtggtgttt attggggaaa aagaaaatca ttattacatt cataattgca
     3781 ggaatcattg gtggttttat ttttatgtat tataaggagc aatttcttta tttgttccag
     3841 atgtacgttt tcttgttcag cggaatatat ggtatctctt tgatttctct tttattagtt
     3901 gaaaaaatcg cttcgttaag agaagcgttc aactatatcc aatacattct ttttacattt
     3961 ggattgttgt tttctcttct tttgaccgtt gccaatgtcg aattagcaac tatcgttttt
     4021 tcttcttcac agttagctgt ttcccatgaa ccaccaaaag aaatcgctgg tgaggactat
     4081 catgtatttt atccagtaac catcgggaaa aaccacgtgg attttattta ttccaatcgt
     4141 tttgcatcag cagcagatca ggaattatat gaaactttga ataaaaacgg aagtatatta
     4201 gtcaatgtat cagattactt gaatgaagaa aatgaacagt ttggcagagg gataaaaata
     4261 aatctgaatt atcttaagaa atttcctttg attgatgtct ctggaaggtt gatccaaata
     4321 accgaaaaag aaacgcaccc tgttatattg atccctgaac gatatagaca tacagattta
     4381 aaaaatgacc tggcgcaaat aacagaatat tatcaagaaa tcagtgaaaa agaaccgaaa
     4441 tttctattta taaaaaacaa tcaacccatt tatacattta ttccttctat cccctggatc
     4501 gaacattatc cggttgttga aatactgaca ttgaaaaaca gtatgtattt ggaaagaaat
     4561 attctcagcg gagaaatcta tccaccgtta aagatcaaga taggctcttt gtcgaaagaa
     4621 aggttatttg agctgattga agaaagtcag ttacgtgata acttgcagtt atcatttccg
     4681 tatactcaga ctgaagagat agctgtcaaa gtgttaagca gatcatttca ttacttgata
     4741 cagatatttt tactcacttt cttttctttc ttcttgttga gctatgtcat gttatgcata
     4801 tattttgtct acaattgtcg gaaaatcgct atctttcgtt caagtggtta ttcgctcttt
     4861 gaaacgtata aagatttctt catgatgaac ctcatcaaat ggggaacgac aagtgtaatc
     4921 ttcctttttc ttatagaaag agagcctaaa tatcttttta atattttttt ctttagcttc
     4981 attgatttta ttctttcagt tgtttttatt ttgtctacag aaaagaaaag tcagttattg
     5041 ctgatgaatg gaggttaaca attggagccg ttgatagtga taaaaggtgc gaaaaaaatc
     5101 attcgaaaca aaacgattct tcatttggat cgtttcactg cggatgctgg ggatcttgtg
     5161 actgttgttg gagaaagtgg agcaggtaaa agtacgttat tgaatatatt aggattgaat
     5221 gatcagttca gttcaggaca attctatttg ttcgatcaag agacgaaaca attaactggg
     5281 agagagaagc gcttgataaa aagaaatacc atatcttttc tttttcaaga ttttgggttg
     5341 attgaatatg aaacaatcga ttttaattta gatattggat tgaagttttc gaatttaaat
     5401 gtttcagata agcgaacagc aaagtatgag gcattgaaaa aagtaaatat aaacaagaag
     5461 ctaagtacgc ctatctacac attatctggt ggtgagaagc aaagaatcgc ctgtgcgaga
     5521 gtcttattga agaatagcaa aatcatttta gccgatgaac cgactgggtc attggataaa
     5581 agaaataagg ataaagtagt tgaaatctta tcgcaacaag cttcccaagg tacgttagtt
     5641 atcgtagtca cgcatgatcc atatctgacc aaaataggaa acaagaagat catgctttaa
     5701 aaatcaaaaa ggaaaggatg tcgttttatg aaaaaagcag ttattatttg tgcactaggg
     5761 ataggttttg ttggaggaaa cgctatgtca tctgaagtgg aagcaaattc agttcacggt
     5821 gaaatctatt acaaatcttt aaagccagga gagattcatt ttgatgctaa aggtaatatt
     5881 attaagtcaa atttaccagt agaccgggaa atttctagaa cagcaattaa tcatagctct
     5941 ggaagatggg tttattatag tgatacaact tgtacgagaa aaacaggtca ttctaaccac
     6001 tatagtcgtg tctataatcg tcacggtgca aaagcgaaag ttggaaactc tattcgatca
     6061 gcaaatgcaa caaaaggagt atgggcctat gcaagtgcga caggaaatag aactgacaca
     6121 ttctcatgtt ggtacaatcc aaccggttgg tattaagtat ccaaaagttt atataaaata
     6181 gaaaaaacat ataatgaaac agcgttaaaa gttatttttg gtagttttaa tatattcatt
     6241 tttagttgta taaaaatata tcataataaa aacaacaaaa aaatgtctgg aaaatcgaac
     6301 ataaaagtgt ctgatttgaa tatcccagca gcggaagaag aaaaacaaaa tcgataacat
     6361 ttgttctaag aattagttac gttaaatacg gaacgcaaga tagacttgaa aaacggtaac
     6421 aaaagaagta gtatattaag gtgtgatgtg ctacttgaca tttattctgc tgagcgaact
     6481 ttctggattg tgtagcctta tatctattca ggacgtatcg agcagattag taaaggtgtt
     6541 cttgatgatt ttcatcttga acacctttgt ttttagcaat tcgcaaaaaa cgaaaagtag
     6601 gagaattgat aaatgaaaaa aagattttta gcacttgcca tcgtactagg cactggctta
     6661 ttgtcaggct gtacgaatgc cggtgaaaaa acagccgtta gttataaagg cggtacaatc
     6721 agcgaacaag aagtaatgga tagcttgaaa aaaatgcaag gagcagattc cgctgtgcaa
     6781 cagttgatcg tttatcaagt atttgaagat aaatatggag atgacgtatc aaccaaagaa
     6841 atcgattctc aatatgatca aacaaagaaa caattgggtg attcatttga ttcacaattg
     6901 aaatctgcag gttacacaga acaaacattc aaagacagta tcaaacaaag tctagccttt
     6961 caagaaggtc taaagaaaca tatcaaattg accgatgaag atttaaaaac ggcttgggaa
     7021 tctttccatc cagaagtgga agctcaaatt atccaagtag caagtgaaga tgatgcaaaa
     7081 gatgtgaaga aagctgctga taagggagac gatttctcta aattagccaa agacaagtca
     7141 actgatacta cgacaaaaga agatggcgga aaagtgaaat ttgattccac tactacaacg
     7201 gttccggcag aagtcaaaga agcagccttt aagttgaaag atggtcaagt ttctgatgtg
     7261 atcacttcta ccaatgcttc aacttatact acggaatatt atgtggttaa aatggtgaaa
     7321 aaccaaaaca aaggcaacga catggataaa tacaaaaaag aattgaaaga aattgcaaca
     7381 gacacaaaat taagtgacag taccttccag aataaagtaa ttggcgaagt attgaaagac
     7441 gctaatgtaa aaatcaaaga caaagacttt gaaaatgttt tgagtacatt cacttcagat
     7501 tctagtacag caagtagtaa ataagcggta taaata
//
LOCUS       DAIGOK010000118         7408 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.119, whole genome shotgun sequence.
ACCESSION   DAIGOK010000118 DAIGOK010000000
VERSION     DAIGOK010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.119"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            1..369
                     /locus_tag="NOA01_002229"
     CDS             1..369
                     /locus_tag="NOA01_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCI0633403.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            549..1490
                     /locus_tag="NOA01_002230"
     CDS             549..1490
                     /locus_tag="NOA01_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCI0633404.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1559..2290
                     /locus_tag="NOA01_002231"
     CDS             1559..2290
                     /locus_tag="NOA01_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HCI0633405.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2434..4614
                     /locus_tag="NOA01_002232"
     CDS             2434..4614
                     /locus_tag="NOA01_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCI0633406.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4634..5116
                     /locus_tag="NOA01_002233"
     CDS             4634..5116
                     /locus_tag="NOA01_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HCI0633407.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5132..5893
                     /locus_tag="NOA01_002234"
     CDS             5132..5893
                     /locus_tag="NOA01_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCI0633408.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6024..7358
                     /locus_tag="NOA01_002235"
     CDS             6024..7358
                     /locus_tag="NOA01_002235"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCI0633409.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
BASE COUNT         2490 a         1287 c         1636 g         1995 t
ORIGIN      
        1 atgtttacga atgaaataaa aatcatgctt tatgtggacg atgtagaaaa aaatgcaacg
       61 ttttggagag cagttggctt tgcggaaaaa gatcgtcaag aaatggatgg gacgcttata
      121 attgaagtag gagttgagga cagtcaaacc tcatttctgt tatatgatcg agcatttgtt
      181 gagtcccatt cacctggcac agctactagt gccccatctt tgatgtttta cagtaaagat
      241 atctttggac tatataaaaa aatgcagacg caatcagtca cattaggtga tatggtacag
      301 cttggagaag aatatgtctt taactttgct gatcccgatg gcaactactt tgcggttaca
      361 ggtaaataat agaaataaag actttgtttt tctgtgcgaa acaggaaagc gaagtctttt
      421 taattcaaat aataatatta tgtaaacaaa aaactttcca tgcgtaatta tacacaagca
      481 aataaatcgt caaatgcgaa cgccttcttt atactgttta ggaacgaaaa gaaggaagga
      541 agaaatacat ggattatcaa gtactgcttt attacaatta tacaaaaatc gaagaaccag
      601 aggaatttgc caaaaatcat ttagcctttt gcaaatcttt ggatcttaaa ggtcgtattc
      661 tagtagctga agaaggaata aatggtactg tgtctggaac cgttgaaaac acgactgcct
      721 atatgaaagc aatgaaagct gatcctcgat ttgctgatac gatgttcaag atcgatccaa
      781 gcgaaggaca tgcctttaag aagatgtttg taagataccg tccagagctt gtaagtttaa
      841 agctagaaga ggacattgat ccaaaccaag tgacaggtgc ctatctttca ccgcaagaat
      901 ttaaagaagc tattttagat gaacagacag tggtgattga tgcacggaat gattatgaat
      961 atgacttggg acattttcga ggggctgtac gcccagatat acgcagtttt cgagagcttc
     1021 cagactggat ccgcgagcac aaagaagaat ttatggaaaa acgggtagtg acctactgta
     1081 ctggtggcat tcgttgcgag aagttttctg gttggcttgt ccgtgaagga ttcaaagatg
     1141 tcggacagct gcatggcggt attgctactt atggtaagga tcctgaagtc caaggagaat
     1201 tatgggacgg caagatgtat gtgtttgatg aacggatcgc tgtcgatatc aaccaagtag
     1261 ataaacaaat cgtcggtcgc gactggtttg acggaacacc atgtgaacga tatatcaatt
     1321 gtagcaatcc tgagtgcaat cgtcagattc ttgcttccat tgaaaatgaa gaaaaacatt
     1381 taggagcatg tagcatagag tgtgcgaaac atccgcgcaa tcgctatgtt caagaccatg
     1441 gaatctctcc agaagaagca gaactgcggt ttacagcttt aattaaatag aaaacaatag
     1501 ttttgattgt cgaccatttc caatatgcgc tatactagcg ttgaggatgg tgatccacat
     1561 gaaatcattg atttatgcta ttggggatgt ccatggcaaa catgagcttt ttgaaaaact
     1621 tattcactat tatgatccaa agatccatca gcttgtttta ttaggggatc tcaatgatcg
     1681 aggtcctcgt acaaaagaat gttttttatt aggaaagcaa ttagtcgaag aaacaggagc
     1741 catttacctc cggggaaacc atgaagaata ttttttgcaa tttctgcaat caccggagga
     1801 ctggtatgcg ccttatatga gaaatggtgg aaaagaaacg tttgaaagcc tgattcaccc
     1861 aggggcatcg gaggaatact ccccaactga aatctcgatg atgatccaat cgagatattc
     1921 agaactgatt aaatttttag tccagcgccc cttgtattat gaatgggaaa attatctttt
     1981 tgtccatgcc ggtgtggatt tgacaaagaa agactggcgt aagacaagtc taagagactt
     2041 tatttggata agggaaccat ttcatgaagg gaaaaacaat acaggtaaga caattgtatt
     2101 tggacatacg attacaccaa tgctccatgg agatatgcag acaacagacc tctggattca
     2161 ggatcataaa atcggaatgg acggtggggc tgtattcgga gggtcattgc atggggttgt
     2221 ttttgacaag gaaggtattc ttcaagatat tgaatatcag aacaacgaag gaccttggca
     2281 tggaatctga ctaatggaaa gaatgaagga gctgtgactt tgttgcggct ctttttattt
     2341 ttatagagaa tattgtttct ttcagtagtt taacccagga taaaaagtat gatagaataa
     2401 agaagcaatt tttagcagaa aagaggcatt ttaatgacgg aaaatttgaa ccagacgatc
     2461 attcagcttg tacaaaaaga actttcagac tatcgtccta agcagttgac tactgtactt
     2521 aatttgttaa atgaaggaaa cactgttcct tttatcgcgc gttaccgaaa agaaatgact
     2581 ggaagtcttg atgaagtcca gattcgtgaa atcgaagagc ggtatgccta tcttgaaaac
     2641 ttagaaaaac gtaaaactga agtgattcgg ctaatcgatg aacaagggaa actgactccc
     2701 gagttagaag cagaaatcac ccaagccgtg aaaatgcaac aagtggaaga tttgtatcgt
     2761 ccatacaaac aaaaacgacg gacaaaagca acgattgcta aagaaaaagg acttgaacca
     2821 ctcgcgaagt ggctgatgca gcttacagat ggagaggtgc aatctgaagc agaaaaatat
     2881 attgacaaag aaaaagaagt ctcttctgcc gaagaagcgt tgcacggtgc tcatgagatt
     2941 attgctgaac aagtcagcga taatgcaaaa tttcggactt ggattcggtc ttatacgtat
     3001 aacaaaggta tgtatgtcag caatgtcaag gatgagcaag cagatgaaaa aggcgtatat
     3061 gaaatgtatt atgactttgc agaacccgtc cataaaatgg tttctcatcg aatattagct
     3121 actaatcgtg gagaaaaaga agatattctg aaagtattct ttcaagtaga tgaagcagca
     3181 attttagctt acttggatcg ccaaatagtc aaaaatcctg cctctccttc ttcttcattc
     3241 gtcagagaag cctatcagga tagttataaa cgctttattc agccagcaat cgagcgagaa
     3301 ctgcgcaatg aattgactga aaaggcagat gagcaagcaa tcgctatttt cggcgaaaac
     3361 ctccgtaatc ttttgttgca accgccacta aaaggtaaag ttgtcctggg atttgatccg
     3421 gcttatcgta ctggatgtaa actagcagtc gtagatgcga cagggaaagt tttagcaatc
     3481 gaagttattt atcctcataa acctgcagcg caagcaaaaa gagaagctgc tggaccagct
     3541 tttatccaat taattaataa atatcaagta gatatggtag ctatcggaaa tggtacagcc
     3601 agtcgggaat ctgaactttt tgtggctgag cagttgaaat ccgccgacca taaaacctat
     3661 tacgctatcg tcaacgaagc aggcgcttct gtttactccg ctagcgaaat tgcacgaaaa
     3721 gaatttccgc atctacaagt tgaagaaaga agtgctgttt ctattgcccg tcgcctgcaa
     3781 gatcctttag ctgaattagt caaaatcgat cctaaagctg tcggagtagg ccagtaccaa
     3841 catgacgttt ctcaaaaacg tttggctgaa caattggatt ttgttgtgga aacagcggtt
     3901 aaccaagtag gtgtggatgt gaatacagca agcccgcagc tcttgcagca tatatcagga
     3961 ttgaataaaa caactgccca aaacattgtc atttatcgtg aagaaaacgg agaattcacc
     4021 gcacgaactc aattgaaaaa agtgccacgc ttaggaccaa aagcatatga acaagcaatc
     4081 ggattcttgc gagtccctgg tgggaaaaac attttggaca acacaggtat ccatcccgaa
     4141 agttattcta ttgcaaaaga aatattaact tcagtccaac tttcagaaaa agagcttggt
     4201 acagaagaag cagtagaaaa attgacacga ttatccgtag agaaactggc tgaaagccta
     4261 tcagtcggag aagaaacgtt agccgatatt ttagctgggc tgacacaacc tggacgagat
     4321 atgcgtgatg agatgccagc gcccttatta cgtacagatg tattgagtat ggaagacttg
     4381 aaaccaggga tggaattgac tggaacggtc cgtaatgtca ttgacttcgg cgcatttgtt
     4441 gatatcggtg tgaagcaaga cggacttgtc catatatcga aactcagcaa aaaattcgtc
     4501 aagcatccta cagatgtcgt gtcagtagga gatatcgtaa cagtttggat agaacaagtc
     4561 gatacgaaaa aaggtagaat cagtttgacg atgctttccc cttatgaaga gtaacaatga
     4621 ggagtaacaa aaattgacac aagaagaact gcagcagtta gtagaagaga tatcattaaa
     4681 aagcttccag cttcctttca agcaccaagc aatgttcaat ccacgcttac gtacaactgg
     4741 cgggcgctat catttaaacg atcatcattt agattttaat gcgaagctat ttgaagagtc
     4801 agacgaagag acgattgcag gaatcatcaa acatgaactt tgccattatc atcttcatct
     4861 agctggaaga gggtatcagc ataaagatat ggatttcaag tatcttttga aaaaaacagg
     4921 agggttgcgc tatgcaccag ccttgacgaa gaaacagata aagatggagt attatcgatg
     4981 ccaagattgt tctgccacga tctgccgcaa gagaagaatc gatgtacgca aatatcgttg
     5041 tggaagatgt catggagaaa tcaaatggat tgagacaaaa agagaagact ttaatgaagc
     5101 acaaaaaatc agatgaaagg atagaaggcc tttggaaaaa agtgagcaga ggaatctaaa
     5161 agactggaat cagctattaa attttttagc cgcacctgaa gagggaaatt ttgaggaact
     5221 gacggcacct acacttattt tagctggaaa ctgtcttcct atcttagctg ataaagcagc
     5281 acaaatgtat ttaaatggcc aagtagatca attgtttttg gtcggagggg tggggcatgc
     5341 gactagaatc ctttatgaga attttgaaaa gcaaggattt cattttgaag atggcatgag
     5401 tgaaagtgaa atctgtcgtc agtatctaaa agaagtctat gatttgcccg ataaggcttt
     5461 tctgatagaa tcgaagtcta caaattctgg agagaacgcc actttttcac tggagattct
     5521 tcattcctta ggagctgtac cagaaaaaat cttattgatg aatgatccga cactgcaacg
     5581 caggactcga gcaacttttg agaaagtatg gcaaaatgaa caaactattt tcgcgaatgc
     5641 tgtgccattt gtgccggaaa tccttcactt ttctgaagag atcatcttta cagcaaaaga
     5701 actgaatcat caatggccta aggaatattt ccatgcgcta gtattaggag aaatggaacg
     5761 tctccatgac gatgagaacg gctatggtcc gaaaggaaaa gattttattc ctcacattga
     5821 tatatcagag gaagtttggt cttcttacac cagaattaaa gagtcaataa aaacggattt
     5881 ttctcgtact tgaccatttt agcaagcgaa tcctcctttc caaataaatt tggttgaaat
     5941 tttcaatcgt ttataagata atgaggagcg tgttgaatgt aatagctaaa agaatggacg
     6001 ctagataaga gaagaggatt caaatggaaa atcaaaataa attgtatcat tttgttggta
     6061 tcaaaggttc aggaatgagc tccttggctc ttgttttaca tgaacaaggt ctaaatgtcc
     6121 aaggatcaga cattgaaaaa tatttcttta cacaaagaga tttagaaaaa gcaaatatca
     6181 cgattctgcc atttaacgca gataatgtaa aaccagggat gacgatcatt gcaggaaatg
     6241 catttcctga ttcacatgaa gaaatccagc gagcaaaaga attaggtttg gaagtgattc
     6301 gctatcacga ctttattggt cattttatcc aaaattatac gagtatcgct gtaacaggct
     6361 ctcacggaaa aacaagtacg actggactgc tctctcatgt attgagtggt gtccgtccaa
     6421 caagttatct aattggagat ggaacaggac atggtgatcc gcaagcggaa ttcttttcat
     6481 ttgaggcctg tgaatatcgc cgtcatttcc ttgcctattc accagactat gcaatcatga
     6541 ccaatatcga ttttgatcat ccggattatt atacaagtat cgacgatgta tttacagctt
     6601 tccaaacaat ggctggacaa gtgaaaaaag ctatttttgc gtatggtgac gatgcttatt
     6661 tacgcaaatt aaaagcaaat gtgccgattt attattatgg tgtcacagaa aacgatgata
     6721 tccaagcacg aaacatcgaa cgcacaactt ctggttctgc ttttgatgtc tatcatgggg
     6781 atgaatttgt tgggcacttc actgtaccag cattcggtaa acacaatatc ttaaatgctt
     6841 taggtgttat tgcagtagct tatttcgaaa aactggatct gaaagaagtt gcagaagaaa
     6901 tgctgacttt cccaggagta aaacgtcgat tcagtgaaaa gatcgtagca gacatgaccg
     6961 tagtcgatga ttatgcgcat caccctgcag agatcaaagc aacgattgat ggcgcacgcc
     7021 aaaaatatcc agataaagaa ataattgcag ttttccaacc ccatacgttt acgcgtacga
     7081 ttgcattgat ggatgaattt gcagaagcac ttgatttagc tgataaagtt tacttatgcg
     7141 atatttttgg ttcggctcgg gaagaacaag gaaacgttaa gattgaagac cttggagcga
     7201 aaatcaaaaa aggtggagaa gtcatcaaag aaaataatgt atctccattg ttagattatc
     7261 atgatgcagt agtaatcttc atgggtgctg gagatgttca aaaatttgaa caagcgtatg
     7321 aaaaactttt aagcagcacg acgaaaaatg tactgtaata caattactgc agtgtcctta
     7381 taaaaataga aagaggggga gtggcaaa
//
LOCUS       DAIGOK010000119         7378 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.120, whole genome shotgun sequence.
ACCESSION   DAIGOK010000119 DAIGOK010000000
VERSION     DAIGOK010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7378
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.120"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            127..579
                     /locus_tag="NOA01_002236"
     CDS             127..579
                     /locus_tag="NOA01_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633410.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="NOA01_002237"
     CDS             593..1282
                     /locus_tag="NOA01_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633411.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPILPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="NOA01_002238"
     CDS             1407..3758
                     /locus_tag="NOA01_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HCI0633412.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="NOA01_002239"
     CDS             3838..4233
                     /locus_tag="NOA01_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633413.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="NOA01_002240"
     CDS             4558..5175
                     /locus_tag="NOA01_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCI0633414.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="NOA01_002241"
     CDS             5216..5515
                     /locus_tag="NOA01_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HCI0633415.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="NOA01_002242"
     CDS             5549..5716
                     /locus_tag="NOA01_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCI0633416.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6366)
                     /locus_tag="NOA01_002243"
     CDS             complement(5761..6366)
                     /locus_tag="NOA01_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCI0633417.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
     gene            complement(6626..7306)
                     /locus_tag="NOA01_002244"
     CDS             complement(6626..7306)
                     /locus_tag="NOA01_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element ISS1N family transposase"
                     /protein_id="HCI0633418.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWVQEYSKVLYYLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVK
                     YLNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGITA"
BASE COUNT         2488 a         1076 c         1426 g         2388 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattttgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttccat
     6301 cattgccaat tgtcgatcca aattttgatc ttgtgaagat actcgaatat agccaatttt
     6361 tcccataaaa aactcctttt taaaagtgat ataatttaca tgtaaaattt acttgtagtt
     6421 ttactgctat catagcattt ttaaaaataa attttatgta gacttaaaat ttacttcatg
     6481 gagggtttaa atggatgaaa tcaaaagtaa caaaaaagca tttggcattt atttaggaga
     6541 acctagtaca gaacaattaa accaattttt tggttctgtt gcgaagtttg aaaatcaaac
     6601 gcgccctctc tgaactccaa atatcttagg ctgttattcc cattaatacc ttgatttcag
     6661 tagacaccga aaagccgaag agcgttccat ttcttcggtt ctttttatat attcctcgaa
     6721 tggtctccat gcccttaatc gtggaagagg ctgtacggag actttgataa aatttatttc
     6781 gtcgtttaat aggtcgatgg tcttgttcta ttaaattgtt aagatacttc acagttcggt
     6841 gctctgtctt agtatataaa cccacactct gtaactttct aaaggcggag ccaagagaag
     6901 gtgctttatc ggtcacaatt gctttcggct caccaaactg tttatggagt cgttttaaga
     6961 aagcataggc tgcttgcgta tcccgtttct ttcgtaacca gatatctaag gttaagccgt
     7021 ccgcatcaat tgcacgataa agataatgcc aacgtccctt aattttgata taggtttcgt
     7081 ccattttcca tgaatagaag gattgtctat ttttcttctt ccaaagataa tagaggactt
     7141 tgctgtactc ttgcacccaa cgataaatcg tagtatgaca aacatttatt ccacgatcat
     7201 ataacaattc ctgaacttca cgatagctta gattgtaacg caggtagtaa ccaacagcga
     7261 caataatgac gtcttttttg aattgtttgc ctttaaaatg attcattact ctgtcctctc
     7321 tgtctttttt ctcaatttta cactaaaata gattttttgg aaaactttgc aacagaac
//
LOCUS       DAIGOK010000120         7350 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.121, whole genome shotgun sequence.
ACCESSION   DAIGOK010000120 DAIGOK010000000
VERSION     DAIGOK010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.121"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(141..1085)
                     /locus_tag="NOA01_002245"
     CDS             complement(141..1085)
                     /locus_tag="NOA01_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCI0633419.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDMD
                     KQVTFIEGDVSDSQLMERIMKEYQFAYIFHLAAVASVADSVERPVETHRVNFDSALLL
                     LELARKYQSNLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKAEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILSGMERYLKTEI"
     gene            complement(1219..2610)
                     /locus_tag="NOA01_002246"
     CDS             complement(1219..2610)
                     /locus_tag="NOA01_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCI0633420.1"
                     /translation="MLNERFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFVINIFIQFS
                     FIALSGSRTTLVCLLITTFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAHAWAHVPHITEVKKDTSDSSGTTSSNKVDLKRPDTDSGDVSNLRFELWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKALRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            2736..3146
                     /gene="tagD"
                     /locus_tag="NOA01_002247"
     CDS             2736..3146
                     /gene="tagD"
                     /locus_tag="NOA01_002247"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HCI0633421.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGHFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            3322..3735
                     /locus_tag="NOA01_002248"
     CDS             3322..3735
                     /locus_tag="NOA01_002248"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCI0633422.1"
                     /translation="MISISHSIKELFTIKDKNIEINSFQETFYKDKKAIVIEGPLVRT
                     FRHCLFCKESAKQIEKNGKKISMIILNRSGNKQTYLRLRKQHYHCQSCGKYFTAHTYL
                     VAPSCFISKQVHYTILEELTERQALKTIAKRCDFQ"
     gene            3892..4149
                     /locus_tag="NOA01_002249"
     CDS             3892..4149
                     /locus_tag="NOA01_002249"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCI0633423.1"
                     /translation="MTFCPAGEKKDLIAYFMRFTRKAHMGVRYIVTDMNAPYFSLVKE
                     PFPNAQIIIDCFHIGQHLNKFFDRVRKRVMKQLNQKCQRHL"
     gene            4236..5030
                     /gene="istA"
                     /locus_tag="NOA01_002250"
                     /pseudo
     CDS             4236..5030
                     /gene="istA"
                     /locus_tag="NOA01_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343025.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            5114..5308
                     /locus_tag="NOA01_002251"
     CDS             5114..5308
                     /locus_tag="NOA01_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633424.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
     gene            5298..5651
                     /gene="tnpB"
                     /locus_tag="NOA01_002252"
     CDS             5298..5651
                     /gene="tnpB"
                     /locus_tag="NOA01_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCI0633425.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            5753..7300
                     /locus_tag="NOA01_002253"
     CDS             5753..7300
                     /locus_tag="NOA01_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCI0633426.1"
                     /translation="METTDTLLQLLQEAHKTNQAQQQTIQNLTTEIQLLNEKVNYLTN
                     KLFGRSKETLFEETNGQLNLFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLP
                     ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEK
                     EGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRD
                     NITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKD
                     SQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFF
                     EAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWM
                     ATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHS
                     TSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDV
                     QQHFSRN"
BASE COUNT         2518 a         1326 c         1273 g         2233 t
ORIGIN      
        1 agttctcttt ttcatgtaaa atatttaatt atatatttcc taaataaaaa gtaaaacaag
       61 aattaaatag ttggttatac aactttaatt cttgttttac tttttagaat tatttatcca
      121 aagatgcaat cattttttca tcaaatttct gtctttaaat accgttccat tccgctcaat
      181 atatcaaatt taggctgata gcctaaagaa cgaatttttg aaatatccgc taaggaatca
      241 cgaatatccc ctgaacgctc cgcttcatat ttaagcgcta gctctgttcc taagatttga
      301 tcaattgaat ggatcaattc aagtagcgta gtgctttttc ctgtaccgac attgaattgc
      361 tgtcctaaag ctttttcttc cttggcaaca agtaaaagtg cttgtataac atcttctata
      421 taaacaaagt ctctagactg actcccatcg ccaaataaag taaattccgc tttttctcct
      481 gccaattgtt ttttgtaacg atccactaga atcgaaatga ctccgctata tggagagttt
      541 ggattttgat ttggaccata aacgttaaaa aatcggacag cactagtcgg aacatcatat
      601 aaatgacagt aatccaaaac atattgttct gctgcaaatt tatcgatagc atatggtgtt
      661 aatggacgga tgactgattc ttcttttttt ggcagtgttg gttcatcacc ataaactgca
      721 gctgatgaac taaatactaa tcgttttaaa ttagattgat atttccgtgc taactcaagc
      781 aataaaagcg cactgtcaaa atttacacga tgtgtttcta caggccgttc tacagaatct
      841 gccacactag ctacagcagc tagatgaaaa atataggcaa actgatattc tttcatgatt
      901 ctttccatca attgagaatc tgatacatcc ccctcaatga aagtcacttg tttatccata
      961 tccaagtttt ctttttttcc cattgataaa tcgtcaacta ctacgatttt gttttctttt
     1021 cccaaataat tcgctagcgt tgaaccgata aaaccagctc cgccagtaat caatatagtg
     1081 tccatcaaat tcactccaat tgcacatttt cgtatcaaat aatattagtt taccattttt
     1141 atattgtaaa aactataaaa tttgttgaag ttgggataac tagttttctt ttctactttc
     1201 atatacctaa aaatagtttc aattagttac tccttcttct ttagatatag ttttttggat
     1261 agaacccaga tatccccaaa aaataaatga acctatcgta ctaaccaaaa tgagttctgt
     1321 gataaataat gccgaaactg ctattgtaag aatcattaaa atagcaaaaa agtgcttatc
     1381 ttttactaga tttggttgtg tgaaacaata acgcaaggct ttaaagcatt ggataaataa
     1441 gaaggttaac aatgtcaata gacctaatcc tcctgtagca gccatcacta agaaaataaa
     1501 attatgagca gttaaagtat cttttcccat taatgtatat ggtagtttat catgtgcata
     1561 ttcaatatag tagcctggag aacctccgga tatcggctta ctcttgaata tttcaattgc
     1621 acttttccaa agttcaaacc ttaaattaga aacatccccg ctatcagtgt cagggcgttt
     1681 taaatcaact ttattgctgc tagttgttcc tgatgaatca gatgtatctt tttttacttc
     1741 tgtaatatgc ggaacatgtg cccatgcatg agcagttata tctgttacat aatacatcgc
     1801 aattatactt agcaatccta ctactaatgc agttagccat ctttttaaag tattagtttt
     1861 aagaaaacga ctactataac ttgtaaagaa actaaaaaca aaagttgtta ttaataaaca
     1921 aactaatgtg gtgcgtgaac ccgataaagc aataaacgag aattgaataa aaatattaat
     1981 tacaaaaaat attttaaata gaagatttaa atgcttgtaa gtgaccaaat aatatatagc
     2041 aataatgatg acgattaatg atataaccgc accaaaattt ggatctgtat atacaccaaa
     2101 taaacggttg cctaaccacc ctaaacgaac tgggtaatac ttatatttta ttggaatttt
     2161 tatgccatat tgaatgacaa accagtataa tgatactaaa ttagctaaaa accataccaa
     2221 tgaaagtatc caggtaaata ctttgaaaaa aaagttacta ctatagtctt tgccaaactg
     2281 ataaatcata aagaaaaaga ttaattgcca catgattaac ttaatatttt cgctatatcc
     2341 atattttaaa ttcattaaac tgcttactag catcgcgcca ataaaagcaa ttaatagcca
     2401 atcatatttt tttagatttt tttctctaaa tgcaataatt gcatctataa tgatcaagac
     2461 aactcccgct attccagcag gccaaaaaag taaagtatca tatctagtag gtaatagtaa
     2521 atacgggcct attgctcgta acattaaaat aaaaccaaaa taaataaaat accaaccttt
     2581 cacatatttt aaaaatcttt cattcaacat ttttcctcca tccacctatt tctcataaat
     2641 atttcttatc ctttttagga taaactggtc ctttcaattc taacaaaaaa atgctaaact
     2701 aacaatgtat attaatgaat ttcgagaggt gaaagatgaa aagagtaatt acttacggta
     2761 cctttgattt gcttcattac gggcatatca atttactgaa acgagcaaaa caatacggag
     2821 actatcttat tgttgccctt tctacagatg aattcaattg gaatgcgaaa caaaaaaaat
     2881 gttattttag ttatgaaaaa agaaaacagt tacttgaagc tattcgctac gttgatttag
     2941 ttattcctga agaaaattgg gaacaaaaag taaaagatat aaaagaatac catgcagatt
     3001 atttcgtgat gggcgatgat tgggctggac attttgatga acttgaacaa ctaacagatg
     3061 cacaagtgat ttatttacca agaacacctg agatctctac aactcaaata aagaacgaac
     3121 ttaattcttt aaatagtgat aaataatatt ttttaaacaa taaaagaaca atatatctgg
     3181 ataagacgta aaaaaatggc tctataattt ctaggactga tagaattttc tgtcggtcct
     3241 agattttttg taaaaaaaaa cagacataaa aaaattccga tagaatagag tcgtctaaaa
     3301 catcaatctg aaaggaagtt tttgatatcc atttctcatt ctatcaaaga gctcttcact
     3361 attaaagata aaaatattga aattaattct tttcaagaaa ctttttataa ggacaagaaa
     3421 gccatcgtaa tcgaaggtcc attagttcgc acttttagac attgtctatt ttgtaaagaa
     3481 tctgcaaagc agatcgagaa aaacggcaag aagatttcaa tgattatctt aaatcgcagt
     3541 gggaataaac aaacctattt gcgtcttaga aaacaacatt accactgcca atcatgcggg
     3601 aaatatttta ctgctcatac gtatcttgtc gctccttcat gttttatttc gaaacaagtt
     3661 cattacacga tattagaaga gttgaccgag cgtcaagcat taaagacaat tgcgaaacgt
     3721 tgtgattttc agtaacgaca gttcaacgga cattatctgc tctatcggca acgctgcgac
     3781 agaagctaga ctatttacct cattgtttac tattcgacga gtttcgctct ctttcaattt
     3841 tccatgggaa gttctccttt tcttgtatga attgagagac agggaagctc tttgacattt
     3901 tgtccagccg gagaaaaaaa agatttgatt gcttatttca tgcgttttac aagaaaagct
     3961 catatggggg ttcgatacat tgtcacagat atgaatgccc cttatttttc attagtcaaa
     4021 gaacccttcc caaatgcaca aatcatcatt gattgctttc acattgggca acacttgaac
     4081 aagtttttcg atcgtgttcg aaaacgtgtg atgaagcagt tgaatcaaaa atgtcaacgg
     4141 cacctttaaa atgtaggaaa acggcaattt aaaattgttg gtttacctac atttttgtat
     4201 gctcttctta tcccaaatga aatggaggct cagggatgag aaaagacata cgcgaaggag
     4261 tgatgatcta cgtgatcaat gaaatcaaac ccaattatgc tgcactagca aaacaatacg
     4321 actgcgacta ccgaacagtt aagcatgctt acgaagaagc ccaagttaaa gaatcaaaac
     4381 ctccagaacg taagaagcgt ccaagtaagt tagatcccta cagagaaatc atccaagaca
     4441 aaatcaaaga ccaatgcagt gcttacagca ttttcaagtt tattgagcac aaaggatttg
     4501 gaggaagcta cagtttagta aagacctatt gtcgccagat taagcaggcg aaagtacaca
     4561 aggcaaccgt ccgagtcgaa catagccctg ggctttctgc tcaggttgac tggaaggaag
     4621 atatgcgact aatcagtaat cagggcgaaa tttttgagtt caatatcttt ttgtacgtct
     4681 tgccatattc caagaaaaaa tatgtcacac taactttcga ccgaaaacaa gacacactct
     4741 ttgagtgctt agatgatgcg ttttatcaaa ccggtggcgt tcccaaagaa atctggttcg
     4801 ataatatgaa gacagtcgtt gaccattctc gtagtcagtt ttccaaaccg catttcaaca
     4861 aacgttttta tgctttcagt aaggatgccg gcttcacacc aattgcctgt cggccatttc
     4921 gtccgcaaac aaagggctgt gtcgaagctt tagccagaac tgttgaccgc ctacgggtct
     4981 ataatcatga attttatgat gccgttgagc tgtgtcattt agtgaataac cgtaagcgcc
     5041 ccatagaaca gtaccttact aacaaatgca cctcaagata tacttgagct atcaagaagt
     5101 ctggaggttt tctatgtata aattaaatga gattgttcct gtccaattag gaaacgaaaa
     5161 gagtgcacca gaaaaaggta aacccgcggc tgccaagtta gcgatgcgca tcaaaagaga
     5221 gaatggttct gaagttttga tctataacgg tattaacaat tatattcttc aagctatttt
     5281 gaaagaactg ttgtccaatg ctttatgatt tacttcaaac caaacatatt tatattgtgt
     5341 gcggaaaaac agatttgcga aaaggaattg atggtcttgc cagtcttatt caacaagagt
     5401 atcaactcga gctttacgaa gatgccgttt ttttattttg cgggaaccgt caagaccgtt
     5461 tcaaacttct ctattgggac ggtgatggtt ttcttctctg ttataagcgg atagaaaacg
     5521 gcaagttaaa atggcctcgg acgaaagacg aagttcgaac cttaacgaac caacaggttc
     5581 gatggttgtt agaaggatta agtatcgatc aacctcgagc tattttgcca ggaaaaaaag
     5641 gagtttttta agctgggggg ttaacacaaa aaatgtgttg aacccctatt tcttgtgcgt
     5701 ttcttgtggt attctgtaac aaaatcagtt cgattcaagg tggtgaaaaa ttttggaaac
     5761 tacagatacg ttgcttcagt tgcttcaaga agcccataaa accaatcaag cacaacaaca
     5821 aaccattcag aatcttacaa ctgaaatcca actattgaat gaaaaggtca attatttaac
     5881 caacaaatta tttggacgct ctaaagagac tctctttgag gaaacgaatg ggcaattgaa
     5941 tctttttagt gatgaagaaa tttctgtaag tgttccagaa gcagctgcca ccattattcc
     6001 agtaaaagga catcaacgag ttgtaggaac gaaagcagac aaaatcaaac acttacctat
     6061 aacagagaaa gaacatctcc ttcctttgga agaacaattt tgtgaacatt gcggttcaca
     6121 gatgaaagat atcggacgga caaaagtacg agaagaaatt cgtttccatc aggctatgtt
     6181 ggattgtctc acccattatc aacataccta ttgttgtaaa agctgcgaaa aagaaggact
     6241 ttcttctttt aaaaaagcga tcgttcctaa acctttgatt tctaatagct taggttccaa
     6301 tagtttagtg gcggaaacga tccggatgaa atttggtcag aaagttcctg cctatcggca
     6361 agagaactat tggaaacaaa cacatgggtt ggatatttcg agagataata tcaccaattg
     6421 gcatattaaa gcagtgcaaa acgcgttaga ccctttagga gaacgattaa gagtctatct
     6481 taatcaagaa gagattctac acggggatga aacaagttat cgtgtgattg aaagtgccaa
     6541 aacagatacc tattactggc agttttgtac aggaaaagac agtcagcatc ccatcgttta
     6601 ttatcaccac gacgaaagtc gtgctggaga tgttccgaaa acttttctca aggagtttac
     6661 agggtattta cactgtgacg gctatagcgg gtataatgcc gtggaaagtg tacgacttgt
     6721 ctattgtttc gcgcatgttc ggcggaaatt tttcgaagca attccaaaag gaaagaaaaa
     6781 cacggatatt cccgcagctc aagcagtcaa acaattggat aaatggtttg tcctagaaaa
     6841 gaaatggaaa gacttttctc ctgagaagcg attaagctgt cggcaacaag aactgcgtcc
     6901 attatttatt gctttttatg agtggatggc aacgatagat cctgttgcaa aatccaagtt
     6961 ggatgcagcc gttcaatatg cgtgtaaact acgaagtggt tttgaaccta tttttgaaga
     7021 tggtcgttta gaactcacaa ataatcgtgc ggagcgaaat atcaaagagc tggtgatagg
     7081 acggaaaaat tggttgcatt ccacaagtct tgaaggagca cgaacttcag gaatcattct
     7141 gagtgtatat aaaacagcag aacttaacgg attgaatcct gtaaagtatc tagagtttct
     7201 atttgataag ataccaaatc ttcccgtact ttcagcagaa actttagatc aactgttgcc
     7261 ttggaacaag gatgtacaac aacatttttc tcgtaactaa aaacgccaaa cattcccgat
     7321 tctattctat aggtcgggtt atttgacgta
//
LOCUS       DAIGOK010000121         7115 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.122, whole genome shotgun sequence.
ACCESSION   DAIGOK010000121 DAIGOK010000000
VERSION     DAIGOK010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.122"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(<1..500)
                     /locus_tag="NOA01_002254"
     CDS             complement(<1..500)
                     /locus_tag="NOA01_002254"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633427.1"
                     /translation="MVEILKEITTYEIIEKTTGLTCYTYQQANEKYRLHFFCYVFDNQ
                     DELLANYEEINDQIAYSFQSQLKRKIERWNIYFFLFVKAKVAKPIKASIEQDKYATRK
                     VIVDNFREFATNDAQKEAQKEAQKEAQKNKIQKRLLLNLEQTKPVKKLAKDERQNDEK
                     IIHLLA"
     gene            complement(516..1355)
                     /locus_tag="NOA01_002255"
     CDS             complement(516..1355)
                     /locus_tag="NOA01_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633428.1"
                     /translation="MYVEREGKCYLTGIITKLQSAGQQGIVEGIHIAHIVAFFLEEFD
                     IMLVPRCLNDFSEYLASIINELREVGGQENKLIFLIEKCYRECFSDITPKLIHNRLSD
                     YLFLPSQKNEDYTNKFLWIAWLEILLYKLLQTNIAFEIDDCFKLLTQEKERAGIHVLF
                     TNHLTLDRFIGSLFRSTLYDKLDKQDVLFVTNQRRKFRGGSIAKREQIEGIVVNIDHP
                     EVSDRLTIDNPNEKKLFPIVHVDYIENEIDQILHDTKGLTAVEFSQRFPEELTRLFEE
                     IAN"
     gene            complement(1808..2902)
                     /locus_tag="NOA01_002256"
     CDS             complement(1808..2902)
                     /locus_tag="NOA01_002256"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633429.1"
                     /translation="MQGQDCNEREMITVKALPAYDGDCLIVQYGEEDQRTNLFVDGGQ
                     GQQVVRQLKEEIATISQKGECIDLLVLTHIDADHIRGFLSLFSQSSFDKSCIKRVFFN
                     SRKLLSQKFDTKVVYDDQLEIVQEKSEISFKQGESFDRYLENLRIEKMTVIDNSCQPK
                     LLNGAKMTILTPDEASLRKLYTDWEKAIQKETRDQLISGRNVNHQGSVEELIKKAFQE
                     DRAVANGSSISFILEYEGKRLLMLADAHPSTVTAKLKELYGEKELHFDLVKVAHHGSK
                     ANTSDDLLKQIECENYLISSNKGNRHGFPHKEALSRIVYHNYTKKRPTNFYFNYSDAS
                     EGLFTNEEIKKYGIQLIKKKMNERVLTIDI"
     gene            complement(3321..3731)
                     /gene="arsC"
                     /locus_tag="NOA01_002257"
     CDS             complement(3321..3731)
                     /gene="arsC"
                     /locus_tag="NOA01_002257"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HCI0633430.1"
                     /translation="MKKIYFLCTGNSCRSQMAEGYARQLLSASDVEIKSAGIETHGLN
                     PRAVQVMAEDGIDISNQTSDLIDQDYFTQADLIITLCGDAKDKCPTIPVGVQHIHWDL
                     TDPAQAVGTEAEITSAFRKTRDRIKAMVIELKNQ"
     gene            4273..4848
                     /locus_tag="NOA01_002258"
     CDS             4273..4848
                     /locus_tag="NOA01_002258"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633431.1"
                     /translation="MVSLLFGFIALMFFLPLTKTHADVTVTNDCTDPSHSFTASINAD
                     NGKDVSSDYYKVTVGQTESWRRNDPRGHILAIVYLEQLRTYYTPTNAAYSFKDGKLYS
                     QGSTTEITPLKVETNSLVTRSMNVNMINLSTSLTTVSLSKWKSGGSTDYFALNHLDSE
                     TWSRNDDPRGFLVSVLYNGGFSKEYIYYLPI"
     gene            4920..5959
                     /locus_tag="NOA01_002259"
                     /pseudo
     CDS             4920..5959
                     /locus_tag="NOA01_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341471.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            6537..7115
                     /locus_tag="NOA01_002260"
                     /pseudo
     CDS             6537..7115
                     /locus_tag="NOA01_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738373.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2207 a         1497 c         1161 g         2250 t
ORIGIN      
        1 tctgctaaca aatggatgat tttctcatca ttttgccgct catccttagc cagtttcttt
       61 actggttttg tctgttccag attcaataat aatctttttt gaattttatt tttttgtgct
      121 tctttttgtg cttctttttg tgcttctttt tgcgcatcat tagtagcaaa ttctcgaaaa
      181 ttatccacga tcacctttct ggttgcatac ttgtcttgtt caatactggc tttgattggc
      241 ttcgctactt tggctttgac aaaaagaaaa aaataaatat tccatcgttc aatcttcctt
      301 tttaattgac tttgaaaact gtaggcaatc tgatcgttga tttcttcata gttggctaat
      361 aactcatctt gattatcaaa tacataacaa aaaaaatgga gtcgatactt ttcgtttgct
      421 tgttggtacg tgtaacaggt caatcccgtc gttttttcta ttatttcata cgtagtgatt
      481 tcttttaata tctctaccat tgttacctac ctccattagt ttgcaatttc ttcaaacaaa
      541 cgagttaact cctctggaaa cctctgagaa aactcaacgg ctgttaaccc ttttgtgtcg
      601 tgtaaaatct gatcaatttc attttcgata taatcgacat gaacaatagg aaataatttt
      661 ttttcattgg gattatcaat cgttaaccga tcagaaactt ctggatgatc gatgttcacg
      721 acgattccct caatttgttc tcgcttggca atactcccac cccgaaattt tctcctttga
      781 ttagttacaa acaaaacatc ttgtttatcg agcttgtcat aaagtgtact tctaaataaa
      841 ctcccaatga aacggtcaag tgttagatga tttgtaaata atacatggat accagctctt
      901 tccttttcct gagtgagtaa tttgaaacaa tcatcgatct caaaagcaat atttgtttgt
      961 aataacttgt aaagcaagat ctccagccaa gcgatccaca agaatttatt ggtataatcc
     1021 tcatttttct gagaaggaag aaacaggtaa tcacttaacc gattatggat caatttaggc
     1081 gttatgtcgg aaaaacattc acgataacat ttttcaatca gaaaaatcag cttattttct
     1141 tgtccaccta cttctcgtaa ttcatttatg atagatgcca aatattcact aaaatcattc
     1201 aagcaacgag gtaccagcat gatatcaaac tcttctaaaa agaaagcaac gatatgtgcg
     1261 atatgaatcc cttcaacaat tccttgttgc cccgctgact gaagtttcgt aataatccca
     1321 gtcaaataac acttcccttc tctttcgacg tacactcctg atcctgagca acctcgtatg
     1381 ttctcatact ccgtgttgtt agacgatcct atttcattgt ccagtaccaa tttaaaaata
     1441 tctggcttac tgatttgagt tgcctttcct cgataaggag ttccaccgtc ttctttcata
     1501 cactctggat aaccataaaa tacgatttct tgcttatttt ctggttccat tataagcagt
     1561 tcaggataac ctttttttac cttcactttg ataatcgcta aatctagact atctgagcat
     1621 cttggcggtt ctaagatttc tagttcagat tgggtataat caaacagttt gaagtcatta
     1681 attgtttccc caaccacaca atgttttgcg gtgatcacaa aacccgtttc acaatcctgc
     1741 tgatacaaca aacctgaacc ataagtattg gttcctctaa tattcactga taactcttca
     1801 aaatcattca tatgtcaatc gttagtaccc tctcattcat ttttttcttg atcaattgaa
     1861 tcccgtattt ttttatttct tcatttgtaa ataacccctc actcgcatcg gaataattaa
     1921 aataaaaatt cgttggtcgt ttttttgtgt agttgtggta aacaatcctt gataaggctt
     1981 ctttgtgcgg aaaaccatga cgattcccct tatttgaact aatcaaataa ttttcacact
     2041 caatctgttt taataaatcg tcactagtat tcgctttact cccatgatga gccactttta
     2101 ccaagtcaaa atgcagctct ttttccccat atagttcttt taacttcgca gtgacagtac
     2161 ttggatgagc atctgctaac atcaacagtc gtttgccttc atactccaat ataaagctaa
     2221 tactgctccc atttgcgaca gcgcgatctt cttggaaagc ttttttgatc agttcctcta
     2281 cgcttccctg atgattgaca tttcgaccgc tgatcagttg atccctggtt tctttttgaa
     2341 ttgccttttc ccaatctgta tataatttcc ttagacttgc ttcatcaggt gtcaaaatcg
     2401 tcatttttgc cccattgagt aatttgggct gacaagaatt gtcaataact gtcatctttt
     2461 caatcctcaa attctccaaa taccgatcga aagactcccc ttgtttaaaa ctaatctctg
     2521 acttttcttg aacgatctcc agttgatcat cataaacaac ctttgtatca aacttttgcg
     2581 ataacagctt tctagaatta aaaaacactc gtttaataca tgacttatca aaactcgact
     2641 gagaaaacaa actcaaaaat ccccgaatat gatcggcatc aatatgtgtc aaaaccagta
     2701 agtcaatgca ctcgcctttc tggctaattg ttgcaatttc ctctttcaac tgcctaacaa
     2761 cttgttgtcc ttgacctcca tcgacaaaca gattcgtccg ttgatcttct tctccatact
     2821 gaacaatcaa acaatcgcca tcataagcag gtaaagcttt cactgtaatc atttctcttt
     2881 cattgcagtc ctgtccctgc aatctcctcc ttcttctgct ttcttttcgc tgatcttgtt
     2941 attctttact aaccaaaatc acacacaata aacttctatt catttgttat tggcactgga
     3001 attcagctga caagaagagc tttcagtcca ttagttaatt gcgcgaaata cactcttttc
     3061 tttcttatct tactccccat aacattgtta ttgtaaataa attttgattt ttaaacgcca
     3121 agcattctca taaccagaaa taattgcttg attcataact cgccaaataa ttgagaaggc
     3181 gaccaaaaaa tagcacaagc aaaaaaagcc agcaagacca ttttaaggct tgctgacttc
     3241 aattcttgat tgttaaaatt tagcactata cagacaaatg aagctcaaat gatcactcaa
     3301 ttcccaaaaa atactgactc tcactggttc ttgagttcaa ttaccattgc tttaattcta
     3361 tctcttgttt ttctaaaagc tgatgttatt tctgcttcgg ttccaactgc ttgtgcgggg
     3421 tctgttaaat cccaatgaat gtgctgaaca cctacaggaa tcgtcggaca tttatctttt
     3481 gcatctccac aaagcgtaat aatcaagtct gcttgtgtaa aataatcctg atcgatcaaa
     3541 tcagacgttt ggtttgaaat atcaattcca tcctcagcca taacctgaac cgctcgtgga
     3601 ttcagtccat gagtttcaat acctgcgctt ttaatttcca catccgaggc agataataat
     3661 tgacgtgcat atccttccgc catttggcta cgacaagaat ttcccgtaca taaaaaataa
     3721 atttttttca tttttatttc tcctcctgga tatgagcatt caatatagct cggtcattat
     3781 tttattctct aactaaaagc aagcatacag gaatttttgt aggaaagcat caaaaaagag
     3841 tgaaatgatt gaaaagttac gtcatcaatt atcagtttgg tatgttttgg ccatcagttc
     3901 tgatcttctt tttacctgat aaataataaa tggcattatc catggatcgc ctaaaattga
     3961 agaaaattca tcatattcga tgtcatgaat catttcctaa aagaattttt ttactaacga
     4021 atgctaaatg aacgctttgt tttaaacggt agtttgaaga accttattct attgaaagaa
     4081 gctagaataa ccgtagctat tagcacccca cccataattt cactacattt ggttttatac
     4141 taaaaaaatg aaataaattg attgattgaa cacattgaaa cctaaacttg ttcctttaaa
     4201 cttaaattgt aagcgaagat cgtttacata aattatatta aagggaggaa taccataaaa
     4261 aaaaaattac cattggtcag cctattgttt ggtttcattg ctttaatgtt tttcttacca
     4321 ctaaccaaaa cacatgctga tgtcaccgta acaaacgatt gtacagatcc gtcacattca
     4381 tttacagctt ccattaacgc agataatgga aaagatgtct cgtcagacta ctacaaagta
     4441 acagttggac aaacagaatc ttggcgtaga aatgatccta gaggccatat tttagccatt
     4501 gtttatttag aacaacttag gacctactat actccaacta acgctgccta ctcttttaaa
     4561 gatggaaaac tttattccca aggtagcaca acagaaatca ctccgttaaa agttgaaaca
     4621 aattcgcttg ttacgcgaag catgaacgtt aacatgatta atctttctac aagtcttacg
     4681 acagtatcgt taagtaaatg gaaaagtggc ggatcgacag actactttgc gctaaaccac
     4741 cttgatagtg aaacttggag cagaaatgat gatccaagag gctttttagt ttcagtccta
     4801 tataatggcg gattctcaaa agaatacata tattatttac ctatataaat tgagttaaat
     4861 tgtattcgtt tttcctccta taggcgtata ctaaagaaaa cacttgagga gtggggaaca
     4921 tggaaacaga acgccaaatc cgtgaattgg aaaaagcgat gaatacgaca gacaaccgcc
     4981 ggttgtacga gcgtttcctg gcagtcaagt tggttcttga aggccacacc cggaaagaag
     5041 ccgggcacac catcggccgc gacgagcata ctgtcagcca ctatgtgaaa aactactgcc
     5101 ataatggaat ccaaggttta gtctccaaaa aacaaagcgg acgaccttca cggctgacag
     5161 atgaacagga agcggaactg gtccaaatcg tcgcctacca cactccggag gaagtaggtt
     5221 tcaaatcccg tgccaattgg acattggcga ttgtgtgtga cctgattctc cgggaatgga
     5281 gttatggata tacgcaaaaa ggcgtggcgg acctccttca ccggctagga ctaagctgga
     5341 cccgtccgac gtataccctc aaaaaagcgg attccaagaa acaacaggta tttttggaag
     5401 aaaccttccc gagcctaaaa aaaaattgct gaatagcgat attgaccacc tcctattcga
     5461 ggacgaaacc atgatccggg attaccaagc cattggctgc acctggtttc tccgcggcca
     5521 ccagcgaaaa atccccactt atggacaaca caaaggggta aaattgatag gcaccttgga
     5581 ttatgaaacc ggtcggatca tttgtacaga agaaggggca tacgacgcag cggccttcct
     5641 ccgttttctg gagcggacgt tgcgggaata ccccaccgga aaaatcgtga tggtattgga
     5701 taatgccctt atccaccacg ccaagctgat ccagtctttt cttgaagaaa acgctaggcg
     5761 cctggcactc atcttcctcc ctccctacag ccctcagttg aacctaatgg aaggcgtttg
     5821 gaagtggctc aaggaatcgg tcatcaacaa tgtttttttt gaccacgtcc agaaaatcaa
     5881 acagtcggtc cgcggtttct tagccgatgt aaacgagcgt ccccttgagg tgattgaccg
     5941 actgtgcgtg cggatgtaaa gtatttaatt caacctatat agcatacaat acctaggcta
     6001 caaaaatttt ctgacaatca gaaaagatgc tatctatgat tgggatgaaa cacagaaatt
     6061 aaaagtagtt aatgtgattg actactaagc atgaagaacc attaaagcga tcaaataata
     6121 actaatgatg agcctaggga gaaaagattc cctaagcttt ttcattaagt acaaaaataa
     6181 ataaccagtg tttttgaaat tgaacttcac acaatagaaa taccgtcttc taaagtataa
     6241 aaaacgttta taaagactat ccgcttattc actcatttta cagtactttt tccaacctct
     6301 ttctagactt cagccagctc ctccgctatt caagaactga tcgatattcc aaagaaaaat
     6361 tccactatac ttgactctaa aataaaaaaa atgaataaaa tagtaaatta gaatcattga
     6421 aaaatagtct gcatccttta gacttaaatt gtaagcgaaa acgtttacga ctatattata
     6481 ttaacaggag gaatatcatg aaaaaaaaat catcactggt cagtttatta ataggaatgg
     6541 tcgctttagc catcttttta atcccgacca aagcagcaca tgctgacgtc actgtaacaa
     6601 acgattgtac ggacttttca agcatccagg cttcaattaa tgcgaattct tcaggacagg
     6661 gtaacaccaa ttacttcaat attgaacctg gtcaatcaga ctcttggagt agactttctc
     6721 gtactgggtt tatcttggcg attgtccata aaggaatgct aagaacctat tatacgccaa
     6781 cgagtgcttc ttactcttat taagatggta aactttatca aaaaaacaat tcagctgaaa
     6841 tcacaccatt gaaagccgta gtattctctg aggaaaaagc tactaaaatt gtaaatatgg
     6901 tcaatctctc tgatggtaca gttcaggttg cattaagcaa atggaaaact ggcgggtatg
     6961 cagattactt tgaactttct cgtccagccg ttgagagctg gagcagaaac aatgatccaa
     7021 gaggcttttt agtttcattg agagctaaag gcgaaatgaa tgaatacata tattatttac
     7081 ctagcattga aaatatcggt ggaaattatt taact
//
LOCUS       DAIGOK010000122         7022 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.123, whole genome shotgun sequence.
ACCESSION   DAIGOK010000122 DAIGOK010000000
VERSION     DAIGOK010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.123"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..813
                     /locus_tag="NOA01_002261"
     CDS             <1..813
                     /locus_tag="NOA01_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HCI0633432.1"
                     /translation="KKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSD
                     NGQIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAIKRVKSVSSG
                     DNFIMINWRIAETQTSFPWCAAYVEAAILCNKTDVHATSARAIMHYTYPNLNNSQIES
                     KSLNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHAL
                     ALFGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            807..1229
                     /locus_tag="NOA01_002262"
     CDS             807..1229
                     /locus_tag="NOA01_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633433.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            1531..1911
                     /locus_tag="NOA01_002263"
     CDS             1531..1911
                     /locus_tag="NOA01_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCI0633434.1"
                     /translation="MSEQNQNLASNYSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASG
                     DAQRLSENLKIMQKELQKIWQQLR"
     gene            1893..2807
                     /locus_tag="NOA01_002264"
     CDS             1893..2807
                     /locus_tag="NOA01_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCI0633435.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRIAIDQTMKNKKTRTYDQFCNSLKTLGIHCFNRGKNFTYELIHKKK
                     KVRSNRLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTLTP
                     EQPEIRPARRNHEQHVERGTDHQQELSL"
     gene            3047..3736
                     /locus_tag="NOA01_002265"
     CDS             3047..3736
                     /locus_tag="NOA01_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633436.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GFIFYLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQALAQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMETLTRLYRNLPEEQQKEYQEKYEQVKKDVQYVKDTKV
                     EEACSDLFGDTIPFSTSEQERKERQQSVTYERYEILLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(3857..3943)
                     /locus_tag="NOA01_002266"
     CDS             complement(3857..3943)
                     /locus_tag="NOA01_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCI0633437.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            4580..5524
                     /locus_tag="NOA01_002267"
     CDS             4580..5524
                     /locus_tag="NOA01_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HCI0633438.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            5545..6081
                     /locus_tag="NOA01_002268"
     CDS             5545..6081
                     /locus_tag="NOA01_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCI0633439.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTPILSEET
                     ESIEKHTKKKQKWWQLWK"
     gene            6097..6267
                     /locus_tag="NOA01_002269"
     CDS             6097..6267
                     /locus_tag="NOA01_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633440.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            6681..>7022
                     /locus_tag="NOA01_002270"
     CDS             6681..>7022
                     /locus_tag="NOA01_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HCI0633441.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTV"
BASE COUNT         2652 a         1212 c         1238 g         1920 t
ORIGIN      
        1 aaaaaaaata caaatggaga atattcagga agtatctcac aatttttagc aaaagaactt
       61 caaaatctag tagatagtaa tataacagat acaaccttat tttttcaaga taatagcatt
      121 tattattccg ataatggaca aattaaacta ctttggaaat cacctttaag tacagaaaaa
      181 aatggtgttt cttcctttac agaaaaaaat gatcttatat ccgtaaatat aaaaaataaa
      241 attaaaaatt tctctaaaaa acaaaatttt cctgcgatta aacgtgtaaa aagtgtctcc
      301 tccggagata actttattat gattaattgg cgtatagcag aaacccaaac aagttttccg
      361 tggtgtgctg cttacgtaga agctgctatc ttatgtaaca aaacagatgt acatgctact
      421 tcagctagag ccattatgca ttatacctat cctaatttaa acaacagtca aattgaatca
      481 aaaagtctta atcaagatca aataataaaa tatgcaaatt actgtggtgt ttatcctaaa
      541 aaagtagcac gaatactaaa cctttcagag gtccaaacac aactaagaaa aggaaatgcc
      601 atttatatgg gaactgctgg ttatggaaat tatagtaaca gccgtcatgc acttgcatta
      661 tttgggtggg tcaatcaagg tggttatcaa acttactatg tgtggaatcc ttggtataac
      721 tacccaactg taataagtgc aaatgcagga ccagttacaa ttcctgtacc tggaggtggt
      781 tttacttggg acaataccat tacaaattgg taaataaacc cattgcttta gttatgggtc
      841 tagtgttagt tcttttctta actagttgtt caaaaaaaca agaaatacaa aagactgatc
      901 tgattccttt tctaggaact tggcaaacta aagatcattc agaacgtcta gtaatcacta
      961 atttaaatac ttcaaaaaat atagaacaat ataccatctt agtaaaagag aacaatcata
     1021 aaattcaaat cattgataaa aatttaaata taaatgaaca agttttcatt tacaaagcaa
     1081 caaataattt actaacactg cgctctaata aaaaacaatt agattacact tttagtttgg
     1141 attctccaaa aaagctcgta tttattttga caacgaatga tgggacagaa gggacagcta
     1201 aaccaattga attttttaaa gctgattgat gaatagttgt ttgatattac aaaacaataa
     1261 tttcaccgaa ggtgaaacaa gcgaaacttt acaaaaagtg gctagcattt gctagccttt
     1321 tttgtattgt ggagcgtggg ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1381 aagcggttat ttgcaccaaa tacggaggca tttggctagc aaatatagag cttgccaaac
     1441 ccttttagtg agctttactc attgtacctt tttaggtaca atggtattgc gaacgatacg
     1501 tccgtaggac gtactgaaag ggtgggactt atgagcgaac aaaatcaaaa tttggctagc
     1561 aattattcta aaaaatatac ctatcgatcc gagccaaaac aaatcagttt tcgagtgagc
     1621 gaatccgaat ttgcaaagct gaagcaatcg gctgaagctt tgcaaatgag tgtgccggct
     1681 ttcgtgaaag ccaaggcaca aaacgcgcgt ctggtgacac cgaaagtggc gccagacatt
     1741 gcccaggcaa tcgcgcgcga tttagccaaa gctggaggga atatcaatca aattgctaag
     1801 tggtgcaata cacaccaaca tgacgttgct tctggtgacg ctcaacgttt atccgaaaac
     1861 ctaaaaatca tgcaaaagga gctacaaaag atatggcaac aattaaggta agccgaacca
     1921 cttcttgtag tcgtgcaatt aattatgcag aaccacgagc aaccattaaa acaggaataa
     1981 actgtgacgt cgattatgcc aaaagtgaaa tgaaacaaat tcgcatgctg tatggaaaag
     2041 acgaccgtgt acaggcacac ttattgattc aatcttttcg accaggagaa attaccgctg
     2101 aaaaagctaa tcaactaggg gcagagtatg caaaaaaaat agcccccgat caccaaatag
     2161 ctatttacac acatacagac aaagaccata ttcataatca cattgtcatt aactccgtta
     2221 atctggagac tggaaaaaaa tttcaagctc atggtcaagc atttctaaac aaatgttatg
     2281 acctaaatga cgagatttgt cttgcacata acttaccaat cactgagcga ggaaagaagc
     2341 cagaaaaacg aaccatgtct gaaataaaat taaaagaaaa aaatgaacct gtttggaaag
     2401 acgaaattcg gattgcgatc gatcaaacaa tgaaaaacaa aaaaaccaga acctatgacc
     2461 aattttgtaa ttccctcaaa acattgggga ttcattgctt caatcgcggg aaaaatttca
     2521 cttacgaatt gatccacaaa aagaaaaaag tacgatccaa cagactagga aaggactacg
     2581 aaaaggagac gattttacat gagctggaca gacgagaacg aacctataac caacagcgaa
     2641 ccaacgagct acgaagagct cgagctagca ttcgcgaaac tgcagaagaa gtacgaagag
     2701 aacaacgaac tcttacgcct gaacaaccgg aaattagacc ggctcgaaga aaccatgaac
     2761 aacacgttga aagaggaaca gaccaccaac aagaattatc gctctaaaac gttaaaagac
     2821 atagaaaacg aaataaagaa acaacgtgct tttctcgttc cttatacgca agaaaggata
     2881 caaactacga ttgatttaca agttaaaaaa ctccatcaag agctattaga ccagcgaaat
     2941 aaggaacaga aaaaggtgct tattcaacga tcattggatt ttcttgcccc cttatgttgc
     3001 ttagtcctga cactgttcgc attttggtta gtatggggag gctaaaatgg cattttattt
     3061 accctatcta ctcatttttg ttagtatttc aggaagtatt tggctcatct ataaaatttt
     3121 tcaaacaaga tattcattaa aaggctctaa aattcgattt aagcgatttt tctttcttgg
     3181 ctttatattt tacttgataa tcgtttcaag cggcttattc ggcgttttag agggaaataa
     3241 gcgcgtttct agaagtattt tattaggaaa tgtcactcaa aagtatgaat cagcgcgtga
     3301 taaaaagaaa aaagagcaag cattagcaca aaaaatggaa gagtttacca cttgttatga
     3361 ggaaatgaac gatatttttg tgaatcaaga aaaacgtttg acggataaaa atatggaaac
     3421 actcactcga ctttatcgga atttgccaga agaacagcaa aaagagtacc aagaaaaata
     3481 tgaacaagta aaaaaagacg tgcaatatgt gaaagatacg aaagtggaag aagcttgtag
     3541 tgatttattt ggagatacaa ttcctttttc aactagcgaa caagaacgaa aagaacgaca
     3601 acaatcagtc acatatgaac gctatgaaat cttgcttcaa caagcaacga acatacaaaa
     3661 tccaacgaaa aaagaaacgg cattaaatta tctaaaaagt gtcaaggaat ggttagatca
     3721 gcaacagcaa aattgaattt gatcggcaat catgttaaac taaacatgca aatgatcaaa
     3781 cattagatac acgaaagccc cagagaggtc ggattctctg gggctttttt tcaggttgat
     3841 gtcgccttta ttgattttac ttcttgtttc ggttattcaa ccaataactg aataaagaga
     3901 cacaaacttc tataagaagg gggcaaatga acaattcaaa cattagatac acctccttcc
     3961 agtcgcaaga ctgtcggata ggcgacaagt tattatatct taaaaaatta aacttgtgtg
     4021 gtgtttttaa gtatcgctta ttttagggtt gttagggctt gccctgaccg cctgtaaggc
     4081 gctttaaagc tagataagga tttttatcca agactgtctt aaaacggctt agataagcat
     4141 tcagaaccgc tcaaaggcct ctttaccaac atgtttttag caaaggagcc tttaggcgac
     4201 tgcgcagtac aatgtgagag cgtagcgatc acaccttttt ggcgaccgta ggaagcaagg
     4261 ttttggattg gcagttttga aaaatgcgcg aacctgttta aagaaaaaag aagtggtttt
     4321 taggcgggaa aattagcccc cctcttttta tatctctttt taaacctctt tttaaacctc
     4381 tttaccgtaa gctgtgtcct tactctacca atggatacag aatgttatga aggacatttt
     4441 gtctgtttat gaaggacatt ttgtctgttt atgaaggaca ttttgtctgt ttatgaagga
     4501 cattttgtct gtttgaacgt caaatatgaa tgtcttgaca tttaaaaaat gtcattcata
     4561 attggattga ggtgattata tggctaatga gattgtaaaa taccaccatg aattaaatac
     4621 aattccttta agaaaattta caccggttga aatgaatttg tttttttcca tcgtgtctag
     4681 aatgcgtgaa aaaggggata agacagttcg ttttacgttt gaccaactaa aagatttaag
     4741 taactacaaa gcaactgcaa atgtacgatt tgtagaagat ttaaaagcaa cttatgaaaa
     4801 aatcttatct ttacgctttg gtcgtagaag tgagtctggg ctttcttacg atatgtttgt
     4861 aatgtttaca gaatttaaaa tcaacgggga cgtagaaaat ccttacgtag atatacgaat
     4921 ttacgaaaaa gcactgcctt tgttaaataa tttagacgag tgggtacgct attctttaca
     4981 acaattcaac gagctacaaa gtggctatgc aaagactatg tttcgtctgt taaagcaata
     5041 cagaaccaaa ggtttcgcat atttctccaa agaagacttt cttgaattat tagatattcc
     5101 taaaagttat aagcaacctg atatagataa acgagttata aaacctatac gtcaagaact
     5161 aactgcaatc ttcaaaggac taacaatcaa aaagaaatac ggaaaaggac ggggaaaacc
     5221 tgttattggc tatcaattta cgttcaaacc tgagatcaaa aaagcagacg actttaacaa
     5281 acatgcacca accaaagaag accaaaataa agcattggct ttagctgata aacaaaaaga
     5341 atacgaagaa cccgctacaa caattaacga aaaaatcaaa cagaagaaac gagaacttga
     5401 actcctccaa caaatcaaac aactagaaaa agagaaaaaa gctcttgaaa aacaaacaga
     5461 gtccgcactt tcggaaaaag agaaaaaaag tttattatct gatttagata atttattcca
     5521 gtagcaacct aggaggcaac cagtatgcaa ccacaagaaa cctttacaat aaaggaattg
     5581 tcagatcttt ttaagatgag taggcaagca atatcgaaac atatccaaaa attagattct
     5641 tccatgatag ctaaaaatga gcgtggttat aaggttgtat tgcgttctgg agtattgcaa
     5701 cttgctcgca acttagacaa tcaaaggttg ctagataagt tgcataaaca tgaacaaaac
     5761 aacgataaaa atgcttatat accaaacaaa aacgtggttg caaacaagtt gctagatcag
     5821 ttgcgtgaag aaaatgaatt tttacgagaa caacttgccc tcaaagagca aaataattct
     5881 aatatgcaaa aattactcga ccagcaacaa cagctcaccc tacaggcaaa tcaacagatc
     5941 gaaaaattgc aagaacagtt acaattagct tatacggaag aaactccaga agatgaatca
     6001 actcctattc tttctgaaga aacagaatcg atagaaaaac acacaaaaaa gaagcaaaaa
     6061 tggtggcaac tttggaaata actggaggga acattcatga caagaaaaaa tcttctattc
     6121 ggttctattt tactaggaat tctaggattc agtccgatgt tattgtcagt gatcaataaa
     6181 gggaataaac atggtgaatt gaaattgaat ccaaattatt acaacagtcc taacgaagaa
     6241 ttgtttgtaa aagaaacgga taaataataa aaaagactgc tttttcgagc agccagttaa
     6301 aaaaataatg agagtttatt cttgaaaaag cagcgcactt ataaagcgct accaaaaatt
     6361 tatcatattt tatgaaaaat aacaacccag taaaaaatga ctggtaaaaa ttcacaaaga
     6421 aacggaaaga agcaactgca gaaaatcaag ccttaaaaag agctttttac ctagaccaat
     6481 ccaaaatcac aaagtgagcc cctgtatcgg ttgatacaag ggctacttgg catactgtac
     6541 attatgttaa ctagctcgat aaataacgat tatgttccca aaacaatatt ttatgaacta
     6601 taacaaaaat ttgttatcgc atattgtata ttttaattat ataattatag gtgtatacca
     6661 cttaaattta acacaaagac gtgattattt tgaaacccaa aaagaaaatt tatctaggtc
     6721 tcctcttttc cttgatgatt ggaattttta gtataggaat tacaaatacc tatgcttcag
     6781 aaaattctaa tttcaatatc aactcccaac caattgaaga acaagcaatt aattttgcga
     6841 aaactgattt tgaacactcc ataacttcaa ttcttttaat ggaaaataac accgaaactg
     6901 atattaacta tactcttggt tcgccattta aaatgctcga tggagaaaat acggcaaata
     6961 gttctatttt ctttccagta ttttcagacg ctgataatac gattaaatat atttataccg
     7021 tc
//
LOCUS       DAIGOK010000123         6486 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.124, whole genome shotgun sequence.
ACCESSION   DAIGOK010000123 DAIGOK010000000
VERSION     DAIGOK010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.124"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            152..2095
                     /locus_tag="NOA01_002271"
     CDS             152..2095
                     /locus_tag="NOA01_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCI0633442.1"
                     /translation="MLSKRESQLIELLLEEENFQPASFFSEKLNVSNKTIYQDIQKLG
                     QFLNRFELKIEKKPRMGIYLIGTSTKKNDAKIFITSKEAMSTKVKYSSNYRRVYLFAN
                     LIFKTDKKRYEYFEKKFFVSIATLRKDYEEIIKYCRLAGIRITSNHVIDTQKTPEELI
                     QRTFVNYFEKNIGVGNEKCQYHFVFDQAMVKFVDDFVKNFYQQSKQQPTNYIVHSLKL
                     SLLTLIGRVKHGYHSQTNKNPLFVEIKKMELYMVALDFSNGVKDELNCSFTINDLYFI
                     CSLFMGHGVRPFVNLATHSQQIMETTKQFIQDMSTLVEKDLGQDSQLLQSLFAHIEPM
                     VYRLQMGITIKNPLKEDIIRQYSTMYTLATYCIPKLEKAMGIRVTDDEITFLTIHFQL
                     AFEKIVNTKHVFLICPTGLGTSQLLFQRIRHSSPSTVVYEVIDVQKLQDYNLDSVDLI
                     ISTVKLENEYQTPVIYVRPLPTKEEIALINRHLLTLNQQDNTFLPSIAPDKTILKKYL
                     KDGWMFLNQDFDSRESVLSFITEKYKEEQLVKDNFLEVVLQREKLGATGLSSGVAIPH
                     ANPKYVVKTKISIITLKNKITWGDVDIRVVLFLAIAEEDMIQVKNMIALLYDVFVSPE
                     RIKSIASKETRNEIINEIIGKRK"
     gene            2097..2558
                     /locus_tag="NOA01_002272"
     CDS             2097..2558
                     /locus_tag="NOA01_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCI0633443.1"
                     /translation="MTSYFGKKIALFQSEVKSQKEALSIIATCLYQTGLVKDSFAENV
                     LKREEVFPTGLLINDVGIAIPHTDAEHVNDSQIAFMSLKTPVTFYEMGTADKEISVSL
                     IFMLALKEPHAQLEMLQKLITMFQKKEVVQKLRAVNNTEEYYQIIEKEGFV"
     gene            2582..3928
                     /locus_tag="NOA01_002273"
     CDS             2582..3928
                     /locus_tag="NOA01_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCI0633444.1"
                     /translation="MLDLLQKFVDLGSIVVLPILIFVFGLILGTKPSKAFISALTVGV
                     GFVGLNLVIDLLTNSLGPAAQAMVERFGLNLTTIDVGWPAAAAISYGTVLGSLAIPVA
                     IVVNVLLIIVGLTKTLNVDIWNIWHAAFVASLVYAVTNDFAMGMLATVIYTMMILLFG
                     DILGPIINKFYGFPNITFPHGTSAPGFVFALPLNWLFDRIPGLKDWHADPETIQKRFG
                     IFGDSTVMGFLIGLVMGILAGYDVAGIGQLAVKTGAVMVLMPKMVALLMEGLTPISES
                     ANSFVQKRFPGRELYIGMDAALSVGHPAVLSSSLILVPITILLAVILPGNTTLPFGDL
                     ATIPFLICLMAAVFGGNIIRTILGGTIYMVTILYITSWVAPFLTKAAQGANFDLQGHS
                     SVTAMAEGGLWTTLLYVGGAKLFGYFALVGIGLIITGLLFYVNRILPKRAAKQTEA"
     gene            3951..4235
                     /locus_tag="NOA01_002274"
     CDS             3951..4235
                     /locus_tag="NOA01_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCI0633445.1"
                     /translation="MKKLLIMCGTGVATSTVVTGKVKEWLKSKGLENEVKLYQSKVAD
                     EIGKIDDYDIVVSTTVVPDNIKDKVIMGLPLLTGIGIDGLFDEIEAKIKA"
     gene            4262..5089
                     /locus_tag="NOA01_002275"
     CDS             4262..5089
                     /locus_tag="NOA01_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005233590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCI0633446.1"
                     /translation="MLVTTKEMFEKAQKENYAIPAANFFDLDSARTFVEVAEKMQKPL
                     ILAFAQAHIEECPLEEAALIATYLAKKASVPVALHLDHGQDEEIIKQAIELGFTSVMI
                     DASQDEIETNIRRSKAIAEYAHERGVVVEAEIGHVGTGNSYEFKETTDTIYTEVEEAA
                     RFAKETGVDSLAVSIGTAHGQYKGTPKISFERLEEIKNEVSIPLVLHGGSSSGDENLH
                     KCAVSGINKINIFTDFITAAMDVINEEKPEDYFTLKRLANQAIADTLSHYYEVFATK"
     gene            5102..5797
                     /locus_tag="NOA01_002276"
     CDS             5102..5797
                     /locus_tag="NOA01_002276"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCI0633447.1"
                     /translation="MRTVRKPFFVVNPKSYLYGEQALKLAKTADQLAAAYDIDIIFTC
                     QHVDVRLIKEQTTQLIVSVQHIDGIVPGRGMGHVLPEGVVAAGAEATFLNHAERPLSV
                     SELCDAVKRAKELGLLTIVCANSLEEAKLVASLKPDVMVCEPTELIGTGQASDISYMI
                     QTNEAVRKMDPEIFVLQAAGISTVADVRKALDSGADATGGTSGIVCAKDPVDTLKAMI
                     EEVSKWKKEQSTK"
     gene            5794..6309
                     /locus_tag="NOA01_002277"
     CDS             5794..6309
                     /locus_tag="NOA01_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016380999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCI0633448.1"
                     /translation="MITYTEQLVLTSNGQRVTYHNITEQVKEIVAKSGIKNGICLVQS
                     PHTTCSVIFEEFMHDLDFNGDEFLQVDLNRILDRIIPRELTENTDYRYPGPKHLDFLL
                     SLDDPDYAGDAGTILNGDAHIRASFFGASENFIVKEEILMTGTVGSIYFIDFDQNRVR
                     NRKCHILVMGE"
BASE COUNT         2149 a          986 c         1375 g         1976 t
ORIGIN      
        1 ttaaaaaaat tgtcgtgagt tttactgaca tcaaaaagta tagggtcata ataatctgtt
       61 ccacattaat gtggaacaga ttacgtattg atctttagtg agaaaaactc tatgatatag
      121 acaaggaaaa cgttttctga ggtgatgatg gatgttatca aagcgcgaga gtcagttaat
      181 tgaattactt ttagaggagg agaattttca accagcaagt ttctttagtg aaaaactcaa
      241 tgtttcgaat aaaacaattt atcaagatat tcagaagctg ggacagttcc tgaatcgttt
      301 tgagttaaaa attgaaaaaa aacctcgcat ggggatttac ttaattggaa cgtcaactaa
      361 aaaaaatgat gccaaaatat ttatcactag taaagaagct atgtcaacaa aggtaaagta
      421 tagttcaaat tatcgtagag tgtacttatt cgccaatcta atttttaaaa cggataaaaa
      481 aagatatgag tattttgaaa aaaagttttt tgtcagtatt gcaactttgc gtaaggacta
      541 tgaagaaatc atcaaatatt gtcggttagc tgggattagg attacttcaa atcatgtaat
      601 agacacacaa aaaacacctg aagagttgat ccagcggaca tttgttaatt attttgaaaa
      661 aaacatcggt gtagggaatg aaaagtgtca atatcacttt gtttttgatc aagcgatggt
      721 taagtttgtg gatgattttg taaaaaattt ttaccaacag tctaagcagc aaccaacaaa
      781 ttatattgtc cattcactca aactttcgtt gttgacgttg attggtcgag taaagcatgg
      841 ctaccattca caaacaaata aaaatccttt gtttgtcgaa ataaagaaaa tggaattata
      901 tatggtggca ctagattttt caaatggggt caaagatgaa ttgaattgtt cctttacgat
      961 aaatgatctt tactttattt gttcgttgtt tatgggacat ggagtgagac cgtttgtgaa
     1021 tctggcaacc catagtcaac aaatcatgga gaccaccaaa caattcattc aagatatgtc
     1081 gacactggtt gagaaggatt tggggcaaga tagtcagctg cttcaatctc tatttgccca
     1141 tattgagcca atggtttatc ggttgcaaat ggggattacc attaaaaatc ctttaaaaga
     1201 ggatatcatt cggcaataca gcacaatgta tacgctagca acttactgta ttccaaaatt
     1261 ggaaaaagca atggggatca gagtaacgga tgatgaaatt acttttttga ctattcattt
     1321 tcagttagct tttgaaaaaa ttgtgaatac gaagcatgtt tttcttattt gtccaacggg
     1381 gttgggaact tcgcaattat tatttcagcg tattcgtcat agttctccta gtaccgtagt
     1441 ctatgaagtg attgatgttc aaaagttgca ggattacaac ttagatagtg tcgatttgat
     1501 tatttcaaca gtcaaattag aaaatgagta tcaaacgccg gttatttatg ttcgaccatt
     1561 accaacgaaa gaagaaattg cattgattaa tcgtcatctt cttaccttga atcaacaaga
     1621 caatacattt ttaccatcca ttgcaccgga taaaacgatt ttaaagaaat atttgaagga
     1681 tggttggatg tttttgaatc aagattttga tagtagagaa tctgtacttt ctttcatcac
     1741 tgagaaatat aaagaagaac aattagtaaa ggataatttt cttgaggttg tacttcaaag
     1801 agaaaaacta ggagctaccg gactttcatc aggcgtagcc atcccacatg caaatcctaa
     1861 atatgtagtc aaaacaaaaa tttctattat cactctaaaa aacaaaatta cttggggaga
     1921 tgtggatata agggttgttt tattcttagc gattgcagaa gaagatatga ttcaagtgaa
     1981 aaatatgatt gcactacttt atgatgtctt tgtctcacct gagagaataa aaagtattgc
     2041 aagtaaagaa acaaggaatg agattattaa cgaaattata ggaaagagga agtaagatga
     2101 cgagttattt tggaaagaaa attgcattat ttcaatctga ggtaaagagt cagaaggaag
     2161 ctttatcaat tattgcaact tgtctatatc aaacaggttt agtgaaggat tcgtttgcag
     2221 agaatgtact gaaaagagaa gaagtgtttc ctactggctt attaatcaat gatgttggga
     2281 ttgctatacc acatacggat gcagaacacg tgaatgattc tcaaattgca tttatgtcat
     2341 tgaaaacacc agtaactttt tatgaaatgg gaactgcaga taaggaaatt tcagttagct
     2401 tgatcttcat gttggcgttg aaagaaccac atgcacagtt agaaatgtta cagaagttaa
     2461 ttacgatgtt tcaaaaaaaa gaggtggttc aaaagcttcg agctgtaaat aatactgagg
     2521 agtattatca aattattgaa aaagaaggat tcgtttaata accattttga gggggagcat
     2581 tatgttagat ttattacaaa aatttgttga tctcggatca atcgttgtat tgccaatctt
     2641 aatttttgtt tttggtttga ttttaggaac aaaaccgagt aaggctttta tttcagcatt
     2701 aacggttggt gttggatttg tcgggttgaa tttagttatc gatttattga ccaatagctt
     2761 aggaccagca gcacaagcga tggttgaacg ttttggacta aacttaacaa ccattgacgt
     2821 tggttggccg gcagcagctg cgatttctta tggaaccgtt ttaggtagtt tggcgattcc
     2881 agtagctatc gtagtcaatg tgttattaat tattgttggc ttaacaaaaa cactgaatgt
     2941 tgatatttgg aatatctggc atgcagcatt tgttgcttca cttgtctacg cagtaacgaa
     3001 tgactttgca atgggcatgt tggcaacggt tatttataca atgatgattt tattgtttgg
     3061 cgatatttta ggaccaatca tcaataaatt ctatggattt ccaaatatta ccttcccaca
     3121 cggaacatca gcacctgggt ttgtttttgc cttaccttta aattggttat tcgataggat
     3181 tccaggattg aaagattggc atgcagatcc agagacgatt caaaaacggt ttggcatctt
     3241 tggtgattca acagtcatgg gctttttaat tggtttggtt atgggaattt tagctggcta
     3301 tgatgttgcg ggaatcggtc aattagcggt gaaaactggt gctgtaatgg tcttaatgcc
     3361 taaaatggtg gcacttttaa tggaaggctt aacacctatt tcagaatcag caaattcatt
     3421 tgttcaaaaa cgtttcccag gaagagaatt atatatcgga atggatgccg cattgtcagt
     3481 aggacaccca gctgtattgt cttcttcgtt aatcttagtc ccaatcacga ttttattagc
     3541 agtgatttta ccaggtaaca caaccttgcc tttcggtgat ttggcaacta ttccattctt
     3601 aatttgtttg atggcagctg tctttggtgg aaacattatt cgaacaatct taggtgggac
     3661 gatctacatg gtcaccattt tatacatcac ttcatgggtg gcaccatttt taacaaaagc
     3721 agcacaaggg gcaaactttg atttacaagg acattcatca gtaactgcga tggcagaagg
     3781 tggactgtgg acaacacttc tatacgtggg tggtgcaaaa ctatttggtt actttgccct
     3841 agtcggaatt ggtctgatta ttacaggctt actattctac gtgaatcgca ttttacctaa
     3901 aagagcagct aaacaaactg aagcttaata caaaatagga gagatgacag atgaaaaaat
     3961 tattaattat gtgcggaaca ggtgtggcta cttcaacagt ggtaacagga aaagtaaaag
     4021 aatggttgaa atcaaaagga ctcgaaaatg aagtgaaact atatcaatca aaagttgctg
     4081 atgagattgg caaaattgat gactacgata ttgtggtcag tacgaccgtg gttcctgaca
     4141 acattaaaga taaagtaatt atggggcttc ctttattaac cggaattggc attgatgggt
     4201 tgtttgacga aattgaagcg aagattaaag cctaaaaaat acttgaaaag aaggtaatca
     4261 aatgttagtt acaacgaaag agatgtttga aaaagcgcaa aaagaaaact atgcgattcc
     4321 ggcagcaaac ttttttgatt tagattctgc aagaaccttt gtagaggttg cagaaaaaat
     4381 gcaaaagcca ttaattttag cctttgcaca agcgcatatt gaagaatgtc ctcttgaaga
     4441 ggcagcgtta attgcaacat atttagccaa aaaagcaagt gttccagtag cacttcattt
     4501 ggatcatgga caagatgagg aaatcatcaa gcaagcaatc gagcttgggt tcacttctgt
     4561 gatgatagat gcttcgcagg atgagattga aacaaatatt cgtcgttcaa aggcgattgc
     4621 ggaatatgct catgaaagag gcgttgtggt agaggctgag attggacacg tgggaacggg
     4681 aaatagttat gaatttaagg aaaccacgga caccatttat accgaagtgg aggaagcagc
     4741 acgttttgcg aaagaaaccg gcgtcgattc attggcagtt tcgataggta cggctcatgg
     4801 gcaatacaaa ggaacaccga aaatcagctt tgagcggttg gaagaaatta aaaatgaagt
     4861 ttcaattcca ttagtgttgc atgggggctc atcatctggt gatgaaaatc tccacaaatg
     4921 tgctgtttca gggatcaata aaattaatat ctttactgat tttatcacag cagcaatgga
     4981 tgtaattaac gaagaaaaac cagaggatta ttttacactt aaacgattag cgaaccaagc
     5041 gattgcggat acgttatcgc attattacga agtatttgca acaaaataga ggagggttct
     5101 catgagaaca gtaagaaaac cattttttgt ggttaatcct aaatcttatt tgtatggtga
     5161 acaggcattg aaactagcaa aaacagcgga tcaattagct gcagcatacg acatcgatat
     5221 catttttact tgtcaacatg ttgatgttcg gctaattaaa gaacagacaa cgcagttgat
     5281 tgtgagtgtt caacatatcg atgggatagt ccctggtaga gggatgggac acgttttacc
     5341 tgaaggcgtt gtagcggctg gagcagaagc gaccttcctt aatcacgcag aacgtccttt
     5401 atcggttagt gagctgtgcg atgccgtcaa gcgagcaaag gaacttggtc tattgactat
     5461 tgtttgtgca aattctttag aagaggctaa gctcgtagct tccttgaaac ctgatgtgat
     5521 ggtttgtgag cctacggaat taataggtac aggtcaagca agtgacattt cttatatgat
     5581 tcaaaccaat gaagctgtac gcaagatgga tcctgaaatt tttgttctgc aggcagcagg
     5641 gatttcaacg gtagcagatg ttcgcaaagc acttgattcg ggggctgatg caacgggtgg
     5701 aaccagcggg attgtttgtg ccaaagatcc tgtggacaca ttgaaagcga tgattgaaga
     5761 agtaagtaaa tggaaaaagg agcaatcgac aaaatgataa cttatactga gcaacttgtt
     5821 ttgacttcaa atggacagcg agtgacgtat cataatatta ccgaacaagt aaaagaaatt
     5881 gtggcaaaaa gtgggataaa aaatggcatt tgtcttgttc agtcgccaca tactacttgt
     5941 tcagttattt ttgaggaatt catgcacgat ttagatttta atggggatga atttttacag
     6001 gttgatttaa atcggattct tgatcgaatc attcctcgag aattaacgga aaataccgat
     6061 tatcgttatc ctggtccgaa gcatcttgat ttcttgctgt ccttagatga tccagattat
     6121 gctggtgatg ctggaacgat tttaaatgga gatgcacata tccgagcatc attcttcgga
     6181 gcaagtgaaa atttcattgt caaagaggaa atactaatga caggaactgt cggctccatt
     6241 tatttcattg attttgatca aaaccgtgtt agaaatcgaa aatgccatat tttagtaatg
     6301 ggtgaataga aaagtaataa gaaacaaaca tttgggaaac taattgaatg atcacaccta
     6361 taaaaattaa tacgaaagat aatagagtca agtccagact cctgtgtaaa atgctataca
     6421 atgtttttac catttctact tatcaaaatt gatgtatttt cttgaagaat aaatccattc
     6481 atcatg
//
LOCUS       DAIGOK010000124         6353 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.125, whole genome shotgun sequence.
ACCESSION   DAIGOK010000124 DAIGOK010000000
VERSION     DAIGOK010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.125"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(98..1228)
                     /locus_tag="NOA01_002278"
     CDS             complement(98..1228)
                     /locus_tag="NOA01_002278"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCI0633449.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="NOA01_002279"
     CDS             complement(1877..3079)
                     /locus_tag="NOA01_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCI0633450.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="NOA01_002280"
     CDS             complement(3139..3342)
                     /locus_tag="NOA01_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HCI0633451.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="NOA01_002281"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="NOA01_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4879..5691
                     /locus_tag="NOA01_002282"
     CDS             4879..5691
                     /locus_tag="NOA01_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCI0633452.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1898 a         1302 c         1003 g         2150 t
ORIGIN      
        1 aataaataat ataaaaggta aataaaaaag agtggatttc tccactccat gaaaagccga
       61 ctttttggga cgcttttttt tgccaagcct cacattttta tagccaaact tcttttgcga
      121 cttgctgcaa taattggatt ttcttccatt gttcttcttc tgttaaatgg tttccttctt
      181 ctgttgaagc aaacccacat tgaggactga tacttagatt ttccaatggt acaaattgtt
      241 gtgcttcttt tattcgttct tttattttct ccgtatcttc caaatctgga aacttagaag
      301 taactaatcc taaaacaact ttactttctt tgttactcca atgtcttaaa ggagtaaaat
      361 ctcctgctcg gtcagaatcg tattccaaaa agaaaccatc ataatttgtt gaaaacaact
      421 cttctgcaat gggttcatat ccaccggaaa acagccaaga agaagcgtga ttcccgcggc
      481 agatatgtgt tgtgatcgtc aaatcttctg gtttgtcttc cagtgcgtta ttgataatct
      541 ctgtacaaat atgggccatt tctttcggat caattccttt ttgaagaagt gcttctcttt
      601 tagtttgatc agctagaaac gcccaatttg tatcatcgaa ttgcaggtaa cggcaacctt
      661 tttcataaaa agcatgaatc gctgctttgt aagtagagga caagtcctca taaaaatcag
      721 atagatcagg ataaatctcc ttgatttttg aagaaccatc attagatagg atctcttgtc
      781 gtaaaatcat cgtgggacta gggatcgttg cttttgcaac aaaagtatca tctgtttcca
      841 ctacttcttt cagaaaagca aaatctttta aaaatgggtg atttggattg aaagataatt
      901 tatctactac ccgaatatta taagctggtg ctgcttttcc gtggaatgtt tgattatatc
      961 cagtttctgg aacatagcct tcgataccat ttagttcttc taaaaaatca agatgccaaa
     1021 atccacgccg aaattcacca tctgtaattc ctttgatgcc gactgctttt tggtcttcca
     1081 ctaatttttt tatttcctgg tcttcgattg cacgaagttc ttcttttgaa agtaagcctt
     1141 cttgaacttt ttttcttgct tctttgatcg attgcgggcg taagaaactt ccgacatgat
     1201 ctactgtaaa tggtctattt ttgctcattt cactcactct tttctttgtt ttttgtaaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattatgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta gaaccggggg ctttattcct gcagtccggc
     5821 tacgccttcc tgcaatagac aaaactccag atcgggtaat tcgcttgtgg cgaaacccga
     5881 cctggagttt gtctctgtat tttgaaccct aaaaattaat caaacagact gatattctca
     5941 taatcagcgc gaaataagcc attaagtaca gttttcctac ctatttttga taacccgctg
     6001 gttagttaat attccttttc cgagagagat aaatgattgg ttaaatctat atacatctca
     6061 acctttaaaa atgttttcga aatacttcat cagtacatgt catacaaatt accagtgcat
     6121 tctcaaattc tagttcttta attgatttca gttgaggagc agttactttt ggcggaaatt
     6181 tccatttttg tccaaacctc ttcccccgat gaaatttctt tgttgcaatt attgcaatga
     6241 aaaatttttg tttcaaaaat atacataatt acttcctcca ataaaaacta tctacctctc
     6301 aaattatttt tctctaataa gaaaaacaga aaaggtaata agatacaaca gtg
//
LOCUS       DAIGOK010000125         6326 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.126, whole genome shotgun sequence.
ACCESSION   DAIGOK010000125 DAIGOK010000000
VERSION     DAIGOK010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.126"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            13..1152
                     /locus_tag="NOA01_002283"
     CDS             13..1152
                     /locus_tag="NOA01_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCI0633453.1"
                     /translation="MNELFRVHLEKAVNTLLKTELTAFLDYEKYDRIGFNTGNSRNGS
                     YDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEETVIHLFRKGITMSEIAD
                     LIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAIYMDATYIPLKRKTVAKE
                     AIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERGLKNVLLFITDGLKGMVG
                     AISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKEVALEARGTFA
                     EKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIESFNKQIKKYSHRKEQFQ
                     NEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQLIEN"
     gene            1286..3475
                     /locus_tag="NOA01_002284"
     CDS             1286..3475
                     /locus_tag="NOA01_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCI0633454.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            3472..4074
                     /locus_tag="NOA01_002285"
                     /pseudo
     CDS             3472..4074
                     /locus_tag="NOA01_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            4182..5369
                     /locus_tag="NOA01_002286"
     CDS             4182..5369
                     /locus_tag="NOA01_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HCI0633455.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
     gene            5498..6326
                     /locus_tag="NOA01_002287"
                     /pseudo
     CDS             5498..6326
                     /locus_tag="NOA01_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
BASE COUNT         2216 a          986 c         1194 g         1930 t
ORIGIN      
        1 aacaaaggcg atttgaatga attattccgt gtccatttgg agaaagcagt caatacgctt
       61 ctcaaaacgg agttaacggc tttcctagat tacgaaaagt atgatcgcat tggttttaac
      121 acgggtaatt ctcgtaacgg ctcctatgac cgtacggtca agaccgagta tggggaactt
      181 catctccaga ttccgcgcga ccgcaatggt gagttcaagc aacagactgt tcctgcttat
      241 agacggacga atgacacgtt agaggagacc gtcattcacc tcttccgaaa aggtattacc
      301 atgtcggaaa tcgcagactt gattgagaaa atgtatgggc attactacac gccccaaacc
      361 atgtccaata taacaaaatc atttacagaa gaggtaacgg cgtttaatgg gcgggagctt
      421 catgaccgtt atgctgctat ttatatggac gcaacgtata ttccgttaaa gcggaaaacc
      481 gtcgccaagg aagctattca tatcgcagtt ggcattcgcc cggacggatc aaaggaagta
      541 ttgagctatg cgattgcacc gactgaatcc atcacgattt gggaggaaat tttattggac
      601 cttcaagagc gcggtttgaa aaatgtcctc ctgttcatca cggatggctt aaaggggatg
      661 gtaggagcga ttagtcggtt ctatcccaaa gctcgttttc aacattgttg tgtacacgtt
      721 tcccgtaata tcagtcataa agtgcgtgtc gatgatcgta aggaagtctg tgatgatttt
      781 aaaatggtgt atcaagcctc atctaaagag gtggcgttgg aagcacgtgg tacttttgcg
      841 gaaaaatgga aaaccagcta tccaaaagtg gttgaatcga ttctttcgaa cgatcacttg
      901 ctcactttct atgatttccc cttggccata cgcaagagta tttattctac gaacttgatt
      961 gaatccttta ataaacaaat caagaaatac agccaccgca aggaacagtt ccaaaatgaa
     1021 gagtcgatgg aacgtttctt agtctcgtct tttgatactt acaaccaaaa attcctaggt
     1081 cgcagtcata aaggtttcca acaggccgaa ggcgagctcg aacaaatgct aagccaactg
     1141 attgagaatt agaaacaatc tgcagtaggg aagaatcatt tacacaaaat tattgacgct
     1201 cccttataat gagaactttt actaacataa tttatgataa acttactata taatcatatt
     1261 aattaaatag ttaggagatc tgcttatgtt tatatctact cttcaaattc gaaattttag
     1321 aaattatgaa tccgaaatct ttcgattcga taatgagaca aatactatta taggtgagaa
     1381 tgattcaggg aaaaccaatg ctcttactgc attgagaatc ttattagatg atagttatta
     1441 ctatagtagt aaaacactaa aagaatcaga tttttttcat ggaattcaaa atgggtggca
     1501 agggcattgg attattatct ctgcgacatt tgaaggaatc agcgaagaag aatttgacaa
     1561 tgagatttgt gcttccattt ctttaaattc tgaaagtcaa acaatcttag aagaattgat
     1621 ttcaaatgca gataaaggtg taggtagtat ttctctattt attagaccta acaaagctat
     1681 tagaaagcaa ttatttgata tttccactga attagactct catcaattta acgaatttag
     1741 aaattctatt cgtctatccg actatgaatt ttactacaca tctaaatcaa atctaaattt
     1801 ctgtatcaat gaaaattatg ataaattggt aggaagactc aatgacaata tagctagtaa
     1861 tcctgaagaa gacgatgaag ctctgttggg ctcaagaatt gacatgtcag atattttcaa
     1921 gcatatttcg gttgtttatg ttgacgctct tcgagatgtt cttagagaaa tgaaaaacaa
     1981 tcgaaaccca gtcaaaagaa tcatggaaac aattgaatca aaaatatcat cagataatgt
     2041 tgagtcatta aaaacaatta tacagcaatt aaatgagact attaccagtg ttcctgaaat
     2101 taggaaaatt ggcgaaaata ttaatcggca actaaatagc ataattggtt cagtgtattc
     2161 tcctaatctt cttctttcct caactatgtc agatgatatg ggttctttag caaaatttct
     2221 ttcaatgaag ccagaacaaa atatcgactt agacttacta gggttaggac atctaaatat
     2281 gatttattta gctttgaaaa tagtcgaatt tgaagcgtgt cgttcaagag aactacttaa
     2341 tattatgttg attgaagaac cagaagctca tattcatcat catattcaaa aaactctgtt
     2401 cgaaggatta aatcttcaaa aaaactatac tcagattctc atgaccacac attctgtaca
     2461 tttagctgaa gtgtctgaaa tatctcggat gaatgtatta aaatcgttta atggcaaatc
     2521 tatcgttatg aatccagcaa ataatctgaa ctcatttgct aaagaaaagt taaaaaagaa
     2581 caatctaaat ctaataaacg cagtagagag atatttagat gttaaaagaa atggattgct
     2641 tttttctaaa ggtgttatct tagttgaagg agatgctgaa gaaatactta ttcctcaaat
     2701 agtaaaaaaa ttatttggta taaacttaga cgaacttgga ataggtttga ttaatgttgg
     2761 tagtacttcg tttgaatata tcgctagctt gtttgacgat gatagaatac agagaaagtg
     2821 tgctattgta actgatttag atgaacaggc aatcccatca gaacatcgat tgtataaaag
     2881 cactgcacaa gagaaaggtc aagcaagaaa agaaaaatta tcctcccttt ttaatgaaaa
     2941 caattgggtt aattgttttt atgctaatac aacatttgaa attgagtttt tagatgtgaa
     3001 taaagaaaat attctaactt atgtcagtcc aattatagat gacgcattca aagataaaaa
     3061 aacaataact gcttataaag aagaaataca aagtgaacaa tatttagaaa gaaatttagc
     3121 gatgttaaaa ctagccaatc atgttggtaa ggggtggttt gcaattgagt taggtgaaaa
     3181 aattgacttt ttaatcaaaa ttcctaagta tattcttaat gctattgctt ttgcatgtca
     3241 ggaaatagta gatctctcaa tttataaaaa aattctactc catacaatcg acttgtacga
     3301 ggaaactaca gaattgtcag ctttatcagc tagtttaaaa acaagtgata cagctgattt
     3361 gaatatagca ataatttctt tattagagta ctttggtaaa gatgaggatg ttgtgtatct
     3421 aatcaatcag atagaaaatt acacaaaaat actttatgct gaggtttcaa aatgacaaat
     3481 atcttcgaaa aaatcgtaaa tgatttaaat ttagaacaaa aagatgttgt ggaaagtgaa
     3541 gataaccttt atgtaaatgc atgtcctggg agtgggaaaa caagggtact tacaaggaaa
     3601 attgcttacc aagcaattaa acacgatgga tcactaaaaa gaataattgc tatcacatac
     3661 actaatagag ctgctgaaga aattaaggaa cgcctaagtt tacttagtat agatgatgat
     3721 attaatattt gggtggggac aatacatcag ttttgtcttg aatttattat ttatcctttt
     3781 aaaatgaatc tctcaagagt atcaaaagga tttacaatta ttgatgatta tactcaaaga
     3841 gcttacgtca gccaaattaa taatatgctg aatttaaatc tcaaaacgta tgaactgaac
     3901 aaaattgatt tgtcactttc tcatgatttt aaaattaatg aagttcagta tactcaagtt
     3961 tctgaaatgt atcacactat gttggaaaaa aataaagaaa ttgattttga tatgatttta
     4021 ctagtagcgt ataaaatttt aactgtaaac agtaacgttg cacgtaatat tgcgattacg
     4081 tccatataat tgtgtaaaag tgaagcatca aaaaagccat tgatctcagt taggagtaca
     4141 atgttttttg cagaaaacat actcgaatag gactgatatc aatgactcaa gtacatttta
     4201 cttttgaaag cgaagaaatt caagcaatta taaacgaaag cggtgcgaat gataccgcaa
     4261 aaacacttat gactatcatg tttaatcagc tgatggaaga acaacgaaat caatatattc
     4321 aggcaaatgc ctatgaacgt tcagaagaac ggcaatcaca aagaaatggt tactacgatc
     4381 gttcgtttac aactcgaatt ggtacgttag aacttcatgt tccacgaacc agagacggta
     4441 agtttagtcc tactatcttt gaaagatatc agcgaagcga aaaagctttg atcgctgcca
     4501 tgattgaaat ggtcatttct ggtgtctcca ctcgcaaagt aactaaaaca gttgaactgc
     4561 taacggatgg tgcgactgtt tctaaatcat tcgtttcaaa tctgatgaaa cagttggatc
     4621 catttgtttt tgagtggaga aaccggagcc tagaaggctc ggaatatcca tttttcatgt
     4681 gtgatgccct gtatatgaaa gtaagggaaa atcatcgtat tgtctcaaaa ggtgtatata
     4741 ttggtatcgg cattgattct gatggacgac gtacgattct tggttttgat gttcaagacg
     4801 gcgaatcgga agataattgg gataccgttt ttcaatcgtt cgttcaacga ggtttgttcg
     4861 gtgtaaaact ggtcatttct gatgcacaca aagggttagt taaggctgtt cgcaagaact
     4921 ttttaggtgc gagctggcaa agatgccaag cccacttttt gagaaatatt tttgataagc
     4981 tgccaaagaa agtttcttct gatgtaaaag acgagttgaa gagtatcttt aaagcctctg
     5041 aacttgaatt gacacgtgaa agaaaagagc acttcttgga aaaatatggt tgtgattcaa
     5101 aattaagtgc tgcttgcgac attttagaaa acggctttga agatgctatt caaattttgt
     5161 cttttcctga aaatatccgc cgaaggattc gtacaaccaa tgtgttggaa cggttgaacg
     5221 aagagattcg ccggagagaa cgagtgattc ggatttttcc aaatatcaat tccatcactc
     5281 ggataattgg aacacttttg atggaaaaag acactgagtg gctggcttct ccacgaaaat
     5341 atttagaatt taattcgaat aacatttagc accatgaacc tgtgcgagtc tcttctttct
     5401 gaggagagac atcaagagtc gctgcgctac tgctcctgac ctctctcctc agaaagaaag
     5461 gcatggcagt taggttcaca acgcaaaaca ttgtgctttt gatgaggttg aacttttaca
     5521 caagattgtg gacttgacta atattgctag taatataagg gcgatttatg tggatgaatt
     5581 tcaagatact agagaattac aatacaatgt aatagctaaa ttacttcaaa acaaccctca
     5641 aattcagtca atgtttgttg gagatattga ccaagcaatt tatggatcat tagatggtat
     5701 agctaaatca gttgaagaac tagaaatgct aactagtcac acttttcaat caaagacgct
     5761 acatggctgt tacaggtcta atcagagatt agtagacttc tactctaatt ttcagagttg
     5821 tccttacgag attgaaagca gagggaataa taaaaacgac cgtggtttta taagttatga
     5881 ttgcaaagtt actaaggagg atttacccaa cataataaaa aatataataa aggaaaagat
     5941 tgaaagcggc attcatgaaa aagatatttg tgtacttgct cctcaacact atcctctgtt
     6001 ttcactgggt gagtatttaa agaaagaatt accgtattgc aattttgatg ctataaatat
     6061 atcaccaatt aaggtaaata atcatagttt atttttcaag ttagcaattt tgtactttac
     6121 tgaatcaggt gaaaaggttc gaagaagaaa gctaattgca aacgatattt taattatatt
     6181 aaaaaatgaa tttggaatag aagttaaaga ttattttatt gacttagatt tattacagct
     6241 aataaacagt gtgaaaagaa tgtatagtgg agacgacaac ggtgttgatt tatacaaatt
     6301 tgttaccaaa catatatttg ttaatc
//
LOCUS       DAIGOK010000126         6321 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.127, whole genome shotgun sequence.
ACCESSION   DAIGOK010000126 DAIGOK010000000
VERSION     DAIGOK010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.127"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            58..477
                     /locus_tag="NOA01_002288"
     CDS             58..477
                     /locus_tag="NOA01_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HCI0633456.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            489..959
                     /locus_tag="NOA01_002289"
     CDS             489..959
                     /locus_tag="NOA01_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCI0633457.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            976..1755
                     /locus_tag="NOA01_002290"
     CDS             976..1755
                     /locus_tag="NOA01_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCI0633458.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            1742..2563
                     /locus_tag="NOA01_002291"
     CDS             1742..2563
                     /locus_tag="NOA01_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCI0633459.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            2580..3629
                     /locus_tag="NOA01_002292"
     CDS             2580..3629
                     /locus_tag="NOA01_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCI0633460.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            3642..4646
                     /locus_tag="NOA01_002293"
     CDS             3642..4646
                     /locus_tag="NOA01_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCI0633461.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            5279..6298
                     /locus_tag="NOA01_002294"
     CDS             5279..6298
                     /locus_tag="NOA01_002294"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCI0633462.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         2080 a         1024 c         1383 g         1834 t
ORIGIN      
        1 attttggcat gcaacttgct tttttagtat gtgtaagtga aaagaggaga tgaaaaagtg
       61 gatagagaaa ttatcttagc ttcccatgga aaatttgctt cagggatatt agattcatta
      121 gagctgatct atgggaaaaa tcattctatt actgtattcg attgctatac tgatgagaat
      181 tttgatttag cggaaacagt aaggcagctt tttatgaggt acaaagataa agaaatcatt
      241 gttctgacgg atctttttgg cggaagtgtc aacaacgaat tcttacaata tatcaatcgg
      301 gatcatgtct atctcgttgc cggattgaat ttgccactat tgattgagct agtatctcgg
      361 ttggacgctg aagtagagac aactgttttg atccagcagg tgctaactga ttcaaaaaaa
      421 atgatccagt tttgtaacga gcggctcaat cagaaaatag aagaagacga attttaggag
      481 gaagaaaaat gattgtattg acaagagtcg accatcggtt attacacgga caagtagctt
      541 tttcatggac acaaacaata ggcgcagact gtattttgat tgctaatgat gatgtaccta
      601 cgaatgagat acgaaagaca acgatcaaat tagccaagcc acaaggagtc aaactagtga
      661 tcaaatcaat tgatgactcg attgccgcgt tgaaaagcgg ggtgacagat aaatataagt
      721 tattcatcgt agtggaatcg atcgaggatg catataagct ggcagaagcg tatccagaaa
      781 tcaaaaccat caacttagga gggatcaagg caaaacctgg tactcgcagc atcggaaaag
      841 cggtaaatat tttaccagaa gaggaggaac gcttgaaacg attaattgac aaacaaatag
      901 aagtagaaat ccgtcaagta ccagcagata aaaaagtgga tgtaagagaa gttttgtaag
      961 aaaggaagaa cctcaatgat tgtacaagca gtcttattag gaattgttgc atttgtcgca
     1021 caatcagaat atgcactagg gacatcgttg ctttctcgtc cgattgtgac ggggctgttt
     1081 acaggaatcg tgttaggtga tgtaaaaact gggatcatta tgggcgcaac tttagagtta
     1141 gcatttatcg gatcgttctc agtaggtgct tcgattccac ctgatgttgt gaccggtggg
     1201 attttaggga cggcttttgc tattacagct ggagccggta cagaaaccgc gcttttacta
     1261 ggtttgccga ttgctacgtt gacattgata ttgaaaaata tttacttagg gcttttgatt
     1321 ccaattatga accataaagc agacaattat gcagaagaag gaaattataa aggagtcgag
     1381 catatgcact tgcttgccgg tatcggtctt tccttgatgc ttggtgttgt ggtaatggtt
     1441 tcttatatgg tgggaagcaa tacgatcgga aatctattaa atatgattcc tgacttcgtt
     1501 caaaaggggt tgtcagtagc tacagggttg attccagctc ttggttttgc gatgcttgct
     1561 cgtttgctga tcaacaaaca agttgcacca tattttttct taggttttgc tatagcttct
     1621 tacttagaga ttcctgtgac cggagtagct atttttggtg cgattttagc agttgtggta
     1681 gtcaatatta tgaatgtccg tcaactacca cttcaaacaa ctgcagggga ggtagaagaa
     1741 gatgaagaat tctgagatca aaaacagcaa aataacgaaa aaagagctaa atcacgtatt
     1801 ttggcgttct tttcaaatgg agttttcttg gaattatgaa cgtcagatga atatggctta
     1861 tgtttatgca atgattccga ttttgaaaaa gttatatcag tcgaaagaag aaatggcagc
     1921 agctttgaaa cggcatctag agttttttaa cacgacccca catatcgtta cactgatttt
     1981 aggaatcacg gctgcaatgg aggaagaaaa taaggatgat cctgaatttg atgtgactgc
     2041 gatcgatagt atcaaaacat ctttgatggg acctcttgca gggttaggag attctttctt
     2101 ttggggaact ttgcgattga ttgccacagg tgtgggtacc tctttagcca tgcaaggaaa
     2161 tattttaggc cctatcttgt tcattttgat tttcaatatt ccgcacattc tgtttcgcta
     2221 tctagcaaca ggttggggat atcgcttagg aacgggtttt ttgaaaaaga tccaggcaaa
     2281 cggtatgatg gaaagtttga cattaggcgc gtctattatt ggactgatgg tcgttggagc
     2341 aatgactgcc acgatgatca ccatcaatat tcctttaaaa atcggttcag gagaaaatgc
     2401 tgtgactgtt caaagtattt ttgatgatat cgttccaaat atcttatgtc ttggagcatt
     2461 tggcatagtg ttttatctat tgaagaaaga agtgaaacca ttaacgatct tgatcggact
     2521 agctttcttt ggaatttttg gttcgtggat agggattttt taaaaaagga gcagagaaaa
     2581 tgtttacgat gcaagattat atctatgaag aaaaggaagt actaagtacg atcttgaaaa
     2641 agaatgattt ttcgacaaga gaacatatga aaaaaacagt gaatttactg atcttagcaa
     2701 cgggttcatc ttataacgct tgtttagcag cgaagccagc attggaatct tatggtgatc
     2761 ttactgtcga tattcaagaa cctttttatt ttttcaatta tggaaaactt tctccttcaa
     2821 tcgatactgt gattgctgtg tcacaaagcg ggaaaagtgc ttctactgtt gaagcagtaa
     2881 aaatgataca aaaacaaggg ttacctgttg ttgctatcac aaatgacgtg caaagtccac
     2941 ttgctttaga agctgcacaa attattgatt taggtgccgg ggtagaatca gtaggcttcg
     3001 ttacaaaagg ctattctgcg actgttttac agttactact cttagggata ggaattggaa
     3061 taagtaaaaa taaaatcagc aaaaaaatag aacaagacta catgcaacag ttgcgcaaaa
     3121 taatcaacca tttaccagcg attattcaaa aaacggaaga attttttgat ggatatcaaa
     3181 gtttatttcg tttagcacag cgtttcgtca ccattggata tggtccaaac tggggaacag
     3241 ctaaagaagc agagacgaaa ttgactgaaa caatccgcgt tccgtcacaa ggatttgagt
     3301 tagaagctta tatgcacggc ccatacctgg aggcagatgc ttcgcatttg ctattcttta
     3361 ttgaagggga aagtgtaaat aaggaacgtt cgcaaaagtt gcaaagatat atgagtcggt
     3421 acgttggaga gactctcacc attaccacaa aaaaagcaag aaacgagaaa acacttggat
     3481 tagcaataga atgtgatgaa tatctgtctg tattagcgct tgtcgttccg tttcaactat
     3541 tcgcttataa aaccgcagta gctaaaggta ttgacttgaa caaaaagatt tttgaagact
     3601 ttgatacagt cttgaaaagt aaattataag aggtggaaat catgctgaaa ttcaatgaag
     3661 aagaacaaat caaagcaata aaaggcgcac tagctttacg tccgcaagta gaagaaatca
     3721 tcgataaact ttatgcggaa aaatttgacg ctgtctatta tctaggaatc ggtggaacgt
     3781 atgcttcctc gatgcaagct gtcacttata tgaatgggaa aagcaacttg cctgtctttg
     3841 tacagcatgc tgcagaatat tacactactg gaaacaaaag attaacaaaa gattctatcg
     3901 ttattttgtc ctctgttacc ggtacaactc aagaagtagt gaaagcagta gaacaaatca
     3961 agaaagtagg agcgacactg attggattta tcgataaagc gaacagtaag ttgtctcagc
     4021 tatgcgattt tgtggtcact tatccagctc ctggaacaga acaaatcaaa ttttttatgg
     4081 cagcagatcg attgatgtat ttgaatggag aattcgaagc ttattcagaa tactatgaac
     4141 agctcgaacg ttatttacca acagggttag tggaagcaga gaaaaaagca gatgcatttg
     4201 gcttatcatt tgcagaaaaa catcgacgtg atagtatgca ttattttatt ggagcaggga
     4261 atcaatgggg agctgtttat tcatatgcca tgtgttactg ggaagaacaa agctggcttc
     4321 catcgaaatc tatccatgct gccgagtttc ttcacgggac gttagagatc gtagaagaaa
     4381 caaccccagt cacattattt ttaggcgaag atgaacagag agtgttatct gaacgtgtgg
     4441 ctaaactttt gccaaaaatt tgcagcaatt atacattgat agacacaaaa gactatccag
     4501 tagaaggtat cagtgaaaaa tatcgcggac gtgttttgtc tttcttattg atgcatgtag
     4561 tgacacaacg catcgatgct catgtggagc agttgaactg ccatccatta gagatacgcc
     4621 gctactatcg tcagtttgat tattaggata gactggataa aaagaatata aaaaataaag
     4681 agagcagcga taactgctcc ctttattttt ttaaaatatt ggcatcaatt acgttataaa
     4741 acacattttt tgcattatct acagttcaac ttataatctg tttaatggga catgtgggtg
     4801 ccaaccattt tttgttatgc aatagtacta tttagttgtt gcatggatcc gtagtaaaag
     4861 tccaagtaca acgagttgcg ccagtttaca tagcgttttg tatccagaat gttgactaaa
     4921 acaaaagttc ctatgtcaac cgtcaacaga gacaatctgt ccgttgcagg acctgacaga
     4981 tttagaaatg ttcaatctgt ctcttatgta ttttaaatca atactcacta ggagaagttt
     5041 cctaacctcc taatagtaat gctggtagta acattgtcaa aactgcttcc tcttaggaac
     5101 aagcaaagca taacgagtgt aaaaataaaa tgtagactat tgtaacatat cattgattat
     5161 ttcaagtaaa aacgataaaa gaagtaaaca aacacttaaa aacaatggaa attagtccca
     5221 actttaagaa atgtatacta taatggaaat tatcaattat ttatttggag gaatatccat
     5281 gaaacaaaaa atcgcagttc ttggtcctgg atcttggggc actgctttag cacaaacatt
     5341 ggcagaaaac gggcatgatg tccgtatttg gggaaatgta cctgaacaga ttgatgaaat
     5401 caataagtat catacaaatc agcatttttt acctgatttg acgatcccag aatcgattat
     5461 cggatataaa gaattggcag aagcagtgga taacgcagat gctattttat ttgttgttcc
     5521 aacaaaagcg attcgttcag tcgcacagga acttattggt aaaatgaata ctaagccaat
     5581 cattatacat gcaagtaaag gactagaaca agatacgcat aaacgaatct cagaagtttt
     5641 ggctgaagaa attccagaag aaaaaagaca ggcaattgtt gtattatctg gtcctagcca
     5701 tgctgaggaa gtggcagtac atgatatcac aacaattaca gctgcaagta ttgatcaaaa
     5761 agcagcagcc tatgtacaaa aactgttcat gaatgattat ttcaggattt atacaaataa
     5821 tgatgtgatt ggcgtagaaa ctggtgcagc actgaaaaat atcattgcaa ttggtgccgg
     5881 cgctatccat ggattaggat tcggagatga tgcaaaagca gcaatcatga ctcgaggatt
     5941 ggcagaaatc agccgtcttg gtgtcgcgat gggtgccaat cctctgacat ttatcggttt
     6001 gagtggtgtg ggggacttga tcgttacttg tacaagtgtt cattctcgca attggcgtgc
     6061 cggtaacttg ttaggacagg gacataagct ggaagaagtt ctagaaaaca tgggaatggt
     6121 cgttgagggt gtggcaacga caaaagcagc agtagagcta gctcaacagc taggtgttga
     6181 gatgccaatt actcaaacaa tctataatgt tttgtataat ggagaagata tcaaaaaagc
     6241 agcaaaagaa atcatgttac gtgacggaaa aatggagaat gaattttctc ttcgttaaat
     6301 agatgatcaa tagttaatcg t
//
LOCUS       DAIGOK010000127         6306 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.128, whole genome shotgun sequence.
ACCESSION   DAIGOK010000127 DAIGOK010000000
VERSION     DAIGOK010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.128"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(67..255)
                     /locus_tag="NOA01_002295"
     CDS             complement(67..255)
                     /locus_tag="NOA01_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCI0633463.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            525..887
                     /locus_tag="NOA01_002296"
     CDS             525..887
                     /locus_tag="NOA01_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCI0633464.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            939..2621
                     /locus_tag="NOA01_002297"
     CDS             939..2621
                     /locus_tag="NOA01_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HCI0633465.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2738..4774
                     /gene="recG"
                     /locus_tag="NOA01_002298"
     CDS             2738..4774
                     /gene="recG"
                     /locus_tag="NOA01_002298"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCI0633466.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4823..5824
                     /gene="plsX"
                     /locus_tag="NOA01_002299"
     CDS             4823..5824
                     /gene="plsX"
                     /locus_tag="NOA01_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCI0633467.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5946..6188
                     /gene="acpP"
                     /locus_tag="NOA01_002300"
     CDS             5946..6188
                     /gene="acpP"
                     /locus_tag="NOA01_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCI0633468.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
BASE COUNT         2099 a         1097 c         1431 g         1679 t
ORIGIN      
        1 ccacccttaa aaatttttat taaataaaaa accgttcact atctgaacgg tccaagtatc
       61 attcaattaa acgcgttcaa cttttcctga tttcaaagca cgagttgaca cccaaacttt
      121 tttaggtttt ccgtcgacca taacgcgaac tttttgtaag ttaggtttaa cagtacgttt
      181 tgttgagttc atagcgtgtg aacggttgtt tccacttttt gtcttgcgac ctgtaaagta
      241 acaaactttt gccatggtaa tttcctcctt tgccttgatt tggagcaata gaataattca
      301 gctcatacca gactaacata tcatatagaa tgggaatttg caagtacatt ttcatagata
      361 caattgacaa ttgagaagaa taattgtaaa ttaaacaagg ttatacctga aattttgcaa
      421 ggtttttgag tactttgggt aactgccttt tatttcaagt tgcgctatga taaaatatga
      481 tagaagaaaa atgggagttt tttacccgtt aaggaggctc attcatggct gtaaaaattc
      541 aaacgtcagc tggaaccatt gaaattacca atgatgtaat tgcaacagtt gttggtggcg
      601 ccgcgacaga tgtgttcggt gttgtaggaa tggccagcaa aaatcaaatt aaagataata
      661 ttactgagat tttgcgtaaa gaaaattact caagaggagt agttgtccgc caagaagata
      721 atggtattgc tgtagatgtt tatacgatta tcagctatgg tacgaaaatc tctgaagttt
      781 cccgcaacgt ccaagaaaaa gtgaaataca atctagaggc attattaggc gtaactgcca
      841 attcagttaa tgtctttgta caaggtgttc gtgtactgcc agactaggca ggagactggc
      901 tggtaactca gcagtcgatc tgaaggagga ttatctaggt gagtgtaaca gaaataagtg
      961 caagccagtt ccaggaaatg gtccaggcag gtgcgaatcg tctgcaagta aatgcagaat
     1021 acgtcaattc attaaatgta ttccctgtgc cagatggcga tacgggaaca aatatgaatc
     1081 tatctatgac tagtggagct aaggcagtag tagattcaac ctcagaaaaa gtaggagaat
     1141 tagcagcaat gctttcaaaa ggattgctga tgggtgcgcg cggaaattcc ggcgttattt
     1201 tgtctcaatt gtttcgtgga ttttctaagc aaattccaga tgttgtctca ttaaatgctt
     1261 ctgatcttgc tgcagccttc actcatggag ttgaaactgc gtataaagca gtaatgaaac
     1321 cagtagaagg aacgattttg acagtagcta gagaagcagc gaaagctgga gaaaagaaag
     1381 caaaaagtac tgatgatgtc gttgaagtaa tgacagctgt tgtaaaagga gcaaagcgtg
     1441 ctttagctaa aactccagac ttgcttccag tcctaaaaga agtcggtgtc gtagacagtg
     1501 gagggcaagg actgctgttt gtttatgagg gctttttaag tgcattgaat ggagaattcc
     1561 aagcggatga aacatatgaa ccttctccag cagaaatgga tgatatggtc aatgcagaac
     1621 atcatcgcag tgtccaaggt caaatggcga cagaagatat ccattttggt tactgtacag
     1681 aaatcatggt aaaaatcgga gaaggaccta cagtcgatag tacattcgat tacgaagaat
     1741 tccgtaacta tttagatggt ataggagatt ctttacttgt ggtcaatgat gacgagatca
     1801 tcaaagtcca cgtgcataca gaaaacccag gagaagtctt gaattatgga caaaaattcg
     1861 gttcattagt caaaatcaaa gtcgacaaca tgcgcttgca gcatgagacg attttggaac
     1921 acgatgaaga agtgaacgag tttgcgaaaa aagaaatacc acgtatcccg tatggtatcg
     1981 tagcgatcgc tgctggtaaa ggcgtccaag agctgtttga aagtttaggc gctaattatg
     2041 ttatcagtgg tggacaaaca atgaatccaa gtacggaaga cattgttaaa gcgattcaag
     2101 aagtaaacgc agataaagtg attgtcttgc caaacaataa gaacatcttc atggcagctg
     2161 atcaagcagc agaagtagca gatatcccag tagcagttgt tccttcaaga acgatttccc
     2221 aaggattgac agctatgctt tcattcaatg atcaagcttc tttagaagaa aacaaagaaa
     2281 cgatgacaga aatgttgtct agcgttgtga gcggtcaagt cactcacgcg atcagagata
     2341 cctcaattga cggtgtagag atcaaagaag gagacttttt gggcatgatc gatggaaaaa
     2401 tcctgatctc taacccagat atcacagcaa cagcgttaag tacactagaa caaatgatca
     2461 atgaggacac agagatcgta acgatcttgg caggtgatga aggaacagtt gaacaagcaa
     2521 atgaatttgc tgagaaacta acagaaaaac atcctgattt ggaaatcgag atccaccaag
     2581 gagatcagcc tgtctatcct tatctatttt ctgctgaata atttctgtgt cagaatatct
     2641 ttctaacaca gtattcattc ataaaaggcc ttcggttagt gatcaggtat gatcgctgac
     2701 caagtccttt ttttgttaaa ggagggagga aaaatttatg cgttctttag atgatccaat
     2761 cactatgctg gcaggagtcg gagaaaaaag agcagctagt cttgccagct tgggcatcca
     2821 aacgattgaa gacttactga cttattatcc ttttcgctac gaagatattc aggaaagaaa
     2881 tctaaacgag atccaggatc aagaaaaggt tactttgaaa ggaatcgtag tctcgcctcc
     2941 tgtgatgaat cgatttggtt ataaaaaaag ccgattgcaa tttcgtatga tgcaagaaca
     3001 tgatgtgttc aacgtttctt tttttaatca accttatcta aaagaaaaag tcattttatc
     3061 ggaggaaatc gccgtatacg gtaaatggga tgcaaagcgt aaagccttga acgggatgaa
     3121 gattttaggt tctcaaacaa tggatgactt ttcgccgata tatcacgtca acaaatcaat
     3181 tcgtcaaacg acattagttg atctaatcaa aaaagggttt caagaatacg gcacattgat
     3241 tgaagaaaat ctgccaaaca accttgtgga gaaatatcga ctgcttgacc gagcttctgc
     3301 tgtaagaagt atgcattttc caaaagacca cgaagaaaac catcaagcca aacgacgtgt
     3361 cgtttttgaa gagttctttt tatttcaaat gcgtcttcaa ggattgaaaa aagcagaaaa
     3421 agcagaaacg aatggtttgg agatccttta tgatgtccaa aaattgaaac aatttactca
     3481 aaagctacct tttgaactga caaatgcaca aaaacgtgta acgaatgaaa tctgtcggga
     3541 tcttcgcagt ccgcagcata tgcagcgact tttgcaggga gatgtaggta gcggaaaaac
     3601 agtggttgcc gcgattgcgt tatacgctac cgttacagca ggtttccaag gtgcgttgat
     3661 ggtgcctaca gaaatcttgg cacaacaaca tatggaaagc ttgcaacagc tgtttgatcc
     3721 caatgaagta accatcgcat tgattacggg gtcaacgaaa gcaaaagaac gacgtgagct
     3781 ccttgaaaaa ctagagcaag gggaaattaa cgtcgtgatc ggcacacatg cattgatcca
     3841 agatggagtc gaatttcaga atttaggtct agttattacg gatgagcagc accgttttgg
     3901 cgtgaaccag cggaaagtac ttcgtgaaaa aggctggcga ccagatgtct tatttatgac
     3961 agcgactccg attcctagaa cattagctat tacagcatat ggtgagatgg atgtatctat
     4021 cattgatgaa cttccagctg gacgtatccc gatcgaaaca cgctgggtaa gaacacctca
     4081 attggacagt gtactggatt ggacgaaaaa agaactggca agaggacatc aaatgtatgt
     4141 catctgtccg ctgattgaag aatcagaagc attagatgta aaaaatgcag tggaaatcta
     4201 tgaaaaactt cgtgaactat ttgcaccaaa atatgaagtt ggcttgttac atggtaaaat
     4261 gaagaatcag gaaaaagaag taatcatgga agccttcaaa gacaaccaga tgcagatatt
     4321 agtttcgact actgtaattg aagtaggggt aaacgtgccg aacgcgaccg tcatgctgat
     4381 catggatgcg gatcgatttg gattggcaca gctgcaccaa cttcgtggac gtgttggacg
     4441 cggaagtgat gcttcctact gtatcttagt tgctaatcct aaaaatgaac taggtgtgga
     4501 acggatgaaa atcatgacag agaccaccaa tggttttgta ctaagcgaaa aagatctgga
     4561 attacgtggt cctggtgaag tatttggctt ccgtcaatct ggtctgcctc aatttgcgat
     4621 tgctgacttg gtgacggatg gaaatgtttt ggaggtagcc agagaagagg ctgcagccct
     4681 ttggcaaata aaggattggc agatgcttcc tgagtatcag cctttagcgc aaagcctcca
     4741 aatagaaaat caggataatc gttatttcga ttagcagact gtgctatact ttatactgat
     4801 gacggaaaaa ggagtggaaa caatgaaaat tgccgtagat gcaatgggcg gagatcacgc
     4861 acctcaagca attgtagaag gcgtggcttt agcgcagcaa gattttccag aagtagaatt
     4921 cttactttac ggaaaagaag cagaaatcaa gaaatactta acaaatgaaa aaaacatcac
     4981 gattatccat acagacgaaa aaatcaatag tgatgatgag ccagtaaaag caattcgacg
     5041 aaaaaagact gcatcaatgg tccttgctgc acaagcagtg aaaaacggtg aagcagatgc
     5101 tgtattttca gcagggaata caggcgcgct actagcagca gggttgttca tcgtaggacg
     5161 tatcaaaaat atcgaacgcc ctggcttgat gtctaccttg ccagtagtag ggaaagaagg
     5221 tgccggtttc gatatgctgg atcttggcgc taacgcagaa aacaaaccag aacatttgtt
     5281 gcaatacggt attttaggtt ctttttatgc acgtaaagtc cgaggtatcg gacgtcctcg
     5341 tgtcgcactc ttaaacaacg gaacagaagc aacaaaaggt agtgaagtga cgaaaaaagc
     5401 ctatgaactc cttcagaatg aaacaagctt gaattttatt ggaaacgtag aagctcgtga
     5461 gctactcaat ggtgtggctg atgtagtggt cacagatgga tttacaggca acgctgtttt
     5521 gaaatctatt gaaggaacag caatgaatat gatgaatcta ttaaaatctg ctattctaaa
     5581 tgaagggatc aaaggaaaaa tgggcgctat gcttttaaaa gacggcttac gttctttgaa
     5641 agcagaaatg gattattcca aacatggcgg tgccgttttg tttggcttga aagcaccagt
     5701 cattaaaact catggagcaa caggccccga tgctgttcgc tatacgatcc gccaaatcca
     5761 cacaatgctt gagacagatg tagttggaca attagtcgaa caatttgaaa aaagcgaaga
     5821 atagtatttg gatatctgaa gcgatccgtt ccattgaaaa aaaggtggac attcctgaaa
     5881 aatgccagta aaattagcca tggtgtaggt aattgacaag tgtatgattc ttggaggtga
     5941 aaaagttgac tcgcgaagaa gtatttaaca aagtagcgaa aattatttca aatcattttg
     6001 agatagatac cgataaagtg acggatgaat tgaacatcaa agatgattta aaagctgatt
     6061 caattagtat tatggaattc gttttagagc ttgaagatga attcggtacg gaaatttcag
     6121 atgaagacgc tgaacaaatt gaaacagtcg gtggcgcagt agattatatc agcagccatc
     6181 ttaaataatt tcaaataaaa gcaaagaagg ataaaaagct gcatgcagct ttttatcctt
     6241 ctttttttaa tctttttgac acgtaaaagt catttttctg ataattctgg cgataaacag
     6301 aaatta
//
LOCUS       DAIGOK010000128         6161 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.129, whole genome shotgun sequence.
ACCESSION   DAIGOK010000128 DAIGOK010000000
VERSION     DAIGOK010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6161)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6161
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.129"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            41..1198
                     /locus_tag="NOA01_002301"
     CDS             41..1198
                     /locus_tag="NOA01_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCI0633469.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(1224..1664)
                     /locus_tag="NOA01_002302"
     CDS             complement(1224..1664)
                     /locus_tag="NOA01_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HCI0633470.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            1824..2330
                     /locus_tag="NOA01_002303"
     CDS             1824..2330
                     /locus_tag="NOA01_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCI0633471.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            2498..4410
                     /locus_tag="NOA01_002304"
                     /pseudo
     CDS             2498..4410
                     /locus_tag="NOA01_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
     gene            4415..5416
                     /locus_tag="NOA01_002305"
     CDS             4415..5416
                     /locus_tag="NOA01_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCI0633472.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(5618..5824)
                     /locus_tag="NOA01_002306"
     CDS             complement(5618..5824)
                     /locus_tag="NOA01_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HCI0633473.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
BASE COUNT         2285 a          941 c         1216 g         1719 t
ORIGIN      
        1 atcaatcctt agaaaatctt agtcaaaaaa ttatataagt ttgcgtacga ttcaaagaaa
       61 attaagagaa cttaatacta ttttgaaaaa ctatcattta tcttgcagct tgaaaaaagg
      121 taatccgata gaaggagaag aataccgtat ccgatacttt tttcacttga tgtattggca
      181 gtcattcgac gaaaatcatt ctgatatatt atcaagtata aaaaataaac gtcaactatt
      241 agaaaaattt gcgaaacatg cgccatatac gcgtagtata gatagaaaga aatggatcag
      301 cctactaaga atttcgttgc aacgaatgaa acaaggccac tcgattacgt atgtcccaga
      361 agagatgaac aaatttgtgc atccattgat aacaaaagaa gagtttacgc attacatact
      421 agagcctttt tttgaagcaa acttctggtc gatgaatcac atcactccaa cagagcaagc
      481 ttacctttat tttatgtttg gtgtaatgaa cacctatttg gaagaagatt tgttggatac
      541 tccagataaa gtacaagaaa gttggggctg gattgaatcg tctattgtag aaaattttga
      601 aaaaaacttc aagatttgtt ttacaacttc agaaaaaaat tatctatatg tcaatttggc
      661 aatgatccat ttgtattcaa gggtttttgg gacaaaaaag aaggtggatg cttttggaaa
      721 atcagcagaa gacagagatt ttcagcatat atttccagtg atgtatccaa tcatgcaaca
      781 gttttttagt ggggtagctc gcagaattcc agagcttaat acttattatg aaaagaacag
      841 acggttgatt tttcaatact gtatgttagt cagggatatt tttataaaac acgaacaacc
      901 tgtcatattc tatattcaat caaagtatgg taaaacacag gaattatggg caaagaaaag
      961 gatattatct atgactgaac gcccaataaa atatagtgca tccgctgatc agttacctga
     1021 tattgtcatt agcgattatc ctatcgacaa aaaggcatat gatcaaacga atacacagat
     1081 attttactgg aatggacaac ctacaaaaaa tgattgggcg cgtctgaaag ctgaaatcat
     1141 aaaaatcagg aatgagaaaa atagatccat attggaaaaa ttggatttcg ttaattaaga
     1201 aaagcagatt gtttatgcca aaattaagac attgcaaaaa gattattttc tttaaaatcg
     1261 acaagatgac cagaaagcaa gagttcgttg agctcttgct ttcttttagt tttagaaagg
     1321 aacatcctga ttttatcttc gctaaatcca gtcaaaagtt ttgcagattg gtcgtagata
     1381 atcggttttt tcaataaaga aggggatgta cgaatctcaa tatatagttc actcaaactg
     1441 agttcatcta ttctgtgaac aagattttta taactaatag aatgagtaga gagcatttcg
     1501 gtcacgccgt tgtttgtcaa ttttagtaag tggataaatt catttttggt tagtggttga
     1561 cgaattaagt tctgttcgat aaacggtata tcatggacga taagccaacg ttttgcgcgt
     1621 cttgaagacg agcagccaga acctgtatac aatttaatca tcatacattc ctccaatagc
     1681 taagtattta tgaaataata acatgaagaa atagtcgaaa agtgccattt agaggtgtaa
     1741 aataagaaaa tgttgtcacg tataatggaa ctagctagat aagaaatact attaaatcaa
     1801 gaaaaaagaa agaaggcaca agtatgtcaa acacagccga cgcaatagta gtgatcgatt
     1861 tacaaaaagg ggtatgtttt gattcttcgc cgatacacga attagaaaca gtgacagcat
     1921 tagtgaataa aagaatcaaa acatacgaaa cgattgggaa accagttatt ttcgttcaac
     1981 acgaagatgc tcaattagtg ccacaaacaa aaccatggga gctattgtcg gaattatcta
     2041 ttcctgagca cagttatttt gtgagaaaaa cccatgcgaa ttcattttat aagactaatt
     2101 tgagtgtttt attaactgaa ttggactgtc attcgatcga attatgtggg gcacaagcag
     2161 agtattgtat agatacgact gtgaagtttg ctcatggatt aggataccag cttatgatgt
     2221 gtagaggagc aacgacaact tatgataatc cttttatgaa agcaaaggaa acaatcgctt
     2281 tttacgagaa aatttgggat aaacgatttc tgacattttt ggagcaataa gttgtttctt
     2341 cgtttaaaaa aagcagaatg gatactggtt tatccattct gctttattac gtaaaatgag
     2401 aaaataatcc tgttctattt ttatgggata aaccatttat tttttgtata aaaaatgttt
     2461 gcgtttacat ataattctcc tagaaacata aaaaggagtg aatagcttga gaacaaaaca
     2521 agtattaaag ggagcgttgg tcatcttatt tgctgtaagt acaagcgtcc cgcaatggac
     2581 agtatatgga gagagcattt cttccgaatc ggaatctcgg gaggtagaaa ccacagaaac
     2641 tactgatgct ggaaatgaaa cgaaatcgag tacttcacaa acagaaacaa atactgaaac
     2701 aacggttgag acatctgtta ctagcacaga tcaagaaacg accgctacca cagataacca
     2761 acaaacagaa gaagcaacag ctacaaaaat gtcaaacgac gaatcctctt ctgctaaagt
     2821 aagtgacaaa gagaatggga cagaaaatga tgatatcggt aaaggtgaaa atttcaatgg
     2881 taaagaatat ttgaattact tcggtaaaca gatgacagaa atacttaatg gaccaacaga
     2941 agacatcaag tcaaaattaa tagagttatt agtcactgag cttgctgtta atgattatgg
     3001 tgtagataaa gagattatca aacatctgat tgctacttat ctattgtacg atatcgcttt
     3061 tgcaaaaaac agtgtgttga tgtcgatgct tggaggtaca gatgatggga caacgcctac
     3121 agaaaacaca ggcttctatc caaatacaaa acaacttcat caaactgtac taaacaaaca
     3181 agatggcaaa atgatggatg tatatgcata ttatgtcgat caaggttcag ataaaacagt
     3241 catgatccat ggcggatttc gagggaattg gaataacggg atcgttacag aagaatacaa
     3301 tgatttttat aaggccgggt ataatttatt atttgttgat tccagagcga ctggtaatag
     3361 cggaggcgat tatgtgacct atggacaata tgagtcagat gacgtccttt actggataaa
     3421 tcaagaagtt cgagagcggc catctcaaaa gattttactt tatggtggtt cgatgggagc
     3481 agctacgatg atgtctgtat tagcgaaaga tattcctgtc aatgtcaaag gaattattga
     3541 aaattgcgga tttgcatcaa ttgatgaaca actacgtttt acgtacagcc agacagtagc
     3601 ccccgcacta ccggatgcca taaaaaatca gttagacatc attggggatc aagagcatga
     3661 agatttattt atgggccttt tgaaacaata ctattttgat caggagatgc atttggatac
     3721 aaaagctgct ctacctacga ttggaatgtc agattcattg cctaaattga ttatccatgg
     3781 aacagcagat gatgtagtac ctgtgtctaa cgcgcaaaaa ttatatgagt tatcaggagg
     3841 gtataaagat cttttattag tagaaggagc agggcatgga aaggcacagg aagttgacca
     3901 tgctgcctac acgaaacatg taacggactt tttgaaagta gtgttccatg atcagataaa
     3961 cgtaaagtat gtggatgaaa acaataaaag tctactcaaa gaccaggacg aaatccagct
     4021 ttacggagct tatggagaaa attatatgac agagcaaaag acattcgaag gatacgaatt
     4081 agcaaatgtt gaaggaccga ctgaagggat ttttaatgag acgataccaa ctattatttt
     4141 ctattacaaa aaaatcccag tagctccacc aaaaaagcaa gatccgacag agaatgcaga
     4201 caataaggga aagaatgaac ctcgagagga tcaatcaagt gatgatacgc aaccgattca
     4261 gatgacagtg aaagtacaaa aaaagacaga tgcaaaaaaa gaatgttgcc gaaaactgga
     4321 gaaaagaaac attcgcttct catttattca ggtggatttt tatctgttag cacgattatc
     4381 acatggattt ggcgaagaaa gagagggtaa atgaatgaaa tggttcaagc gaatagtgat
     4441 tgcagtacca gttatcatcg tagttatttt agtcactgta tttttgatta atcaaatcac
     4501 accaaagccg attagcttgc ttgttagaaa acaatttgac acaagtgaaa aagaacaagt
     4561 atatgcccga ccagatgatt tccagcaaaa aatcgaacaa atcgatataa aaaaagaaat
     4621 atcctatcct tcaaagtaca aaaataataa tatggatatc tacacaccga aagtggaaaa
     4681 gaaaaaatta ccgattcttt tctggatgca tggaggcgca tatgtaggag gggataaaaa
     4741 tgattgtcgt gattatttgg aatatctatg ttcagacacg cagcaaatca tcgtaaatat
     4801 agattatgaa cgatcaccgg aagcaaaaca tcctacacca gttatccaat tgaacgaagc
     4861 cattcaagca gctaaaaaag agataaaaga tcaagcagat tggtcgaata tttcgattgg
     4921 tggaaattcg gcaggtgcac aaattagtgg agaatatttg ttagcacttc aaaatgaaga
     4981 aattaaaaaa gctgatcatc ttgatccgac tttggagaac aagcagatta cgaagtttat
     5041 ttctttgtct ggtctgttgg atccttctgc acttactcaa gtaagtgata aaatctctag
     5101 ctttctttat gaaaaatgtg gatgggctta ctttgataat aaggactttg aacaatcaga
     5161 gaaggtcaaa agtttagcct tggtcagaca tgcggatcgt tggacgcaaa acacattctt
     5221 aacggatgga aatacaaata cgtttacgaa acaaatggaa gaagtaactt ctgtatttga
     5281 gaaagcgggg gtgaaagtca caaaagtaga ttatgcggag aaaaatgctg ttttgaacca
     5341 tgaatatcag tttgatttca gcaataaaca agcgcaagaa acatatcaaa agttagtaat
     5401 gtttttcaag gagtaaagtc ttatgatgca aaagaggttg taatctgtca cttagctcca
     5461 agtcacaagg agcaatttca ataaacaagc ttttcatggt ttatgaatat tgtaacttgt
     5521 gactgaggag ttagagatgg caaggggcac cttttattcg aaatttggga atatcacacg
     5581 attattgtca cgatctcttt tacgtaaatt ttataagtca gatttgttgt tttgatcgtt
     5641 taaaaaaagc aacaaatccg aagcctgcgg aggcaagaag ggacaaaaag ccgagtggta
     5701 agcaaaagaa tgctggttcc actaatattc cgttgttatt gatataaggc tgggaacaaa
     5761 gatgaatagc aaataaaatc aatgcactag cgaaaaaaag caaagaaata tggatagctt
     5821 tcataagcag cacctccttt tttataattg tatgataaat agattaagaa tctataaatg
     5881 tgacaagttt gttaggatgt gaataaaatg atcatctagc gaacttgaaa caaagatggc
     5941 taatgataag caattaatga ttcagcaatc agataggtac aaagtttacc atcatacgtc
     6001 tagaccaata atagaaaata tgggaatata atagaaaaga tttaggatac ccccaaaaga
     6061 atttccccaa aaattcgatg tatcctaaac ttttttattt tacataacaa tcatatcaac
     6121 tgtgaaataa aattacaaat gataacctaa aatgaggcca a
//
LOCUS       DAIGOK010000129         5930 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.130, whole genome shotgun sequence.
ACCESSION   DAIGOK010000129 DAIGOK010000000
VERSION     DAIGOK010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.130"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            287..1246
                     /locus_tag="NOA01_002307"
     CDS             287..1246
                     /locus_tag="NOA01_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCI0633474.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            complement(1210..1668)
                     /locus_tag="NOA01_002308"
     CDS             complement(1210..1668)
                     /locus_tag="NOA01_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCI0633475.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLNFVCQIKLDKSSFVT"
     gene            complement(1681..3012)
                     /locus_tag="NOA01_002309"
     CDS             complement(1681..3012)
                     /locus_tag="NOA01_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HCI0633476.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(3023..4465)
                     /locus_tag="NOA01_002310"
     CDS             complement(3023..4465)
                     /locus_tag="NOA01_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCI0633477.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(4452..5102)
                     /locus_tag="NOA01_002311"
                     /pseudo
     CDS             complement(4452..5102)
                     /locus_tag="NOA01_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
     gene            complement(5121..>5930)
                     /locus_tag="NOA01_002312"
     CDS             complement(5121..>5930)
                     /locus_tag="NOA01_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCI0633478.1"
                     /translation="KKLNHNAVLIDDDGIEKIAMGKGIGFSAKVSKPFDVEDADKLFV
                     LDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARISSPLDSNIYITLTDHIYFAIQR
                     KREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHRYQTELGSNEVGFITMHIINAEL
                     GEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLTYHRLMVHIKFLVKRLIYQEETP
                     VENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQENEIIYLAIHLARIY"
BASE COUNT         1860 a         1205 c          921 g         1944 t
ORIGIN      
        1 ttctaaatca taggagcaac aaaaaaattc gataattaac aaaaatcaaa atgattaaat
       61 ctagtgattg aaacatatca tttcaaaaaa attttgtaaa ttcacccata aatatcaccc
      121 tatttaactt ttttcatcgt acactaaaat tcaaaacaac tatctttaat ctttacttct
      181 tggttttgtt ttcgccgatt gtaaaattaa gctagacaac taaaaaaagt catttgtgct
      241 acactcaaaa tagttccgac gaaagaatta taaggagtga gtacaaatga cctataacca
      301 tcttacacca actgaactcg taatgataga agcttatttc aatcaatcac aacctgtttc
      361 aaaagtagcc aatctgttac aacgttctcg acaaactatt tataaggtct atcgttttct
      421 taaatctggt ggaagcgcaa tgagctacta taaacaatat aagaaaaaca aacgtaactg
      481 cggaagacat ccgattgttt tacctgatga gcaacaggaa tacattcaaa aaaaggttgt
      541 tcaagggtgg gctcctgatg tccttattgg tcgagcagag tttcctattg cttgttcggt
      601 acgcacactg tatcgaatgt ttaaagataa aacctttgac actcacgatt taccaatgaa
      661 gggaaaaaga aaaccaaacg gtcataaaga aaaaagagga aaacaagggt ttcgccggtc
      721 aatccgagac agaaagacag actataccca gtttgatcat gaatttggtc acctggaagg
      781 tgacactatc gtgggtggaa aacataaaag cgcggtcatc acattagttg aacggctatc
      841 taaagtcatc attaccttga aacccgaagg cagacaggct aaagacattg aaaataggct
      901 gaatcaatgg tttcgttgtg tcccaaaaaa cttatttaaa tccatcacat ttgattgtgg
      961 aaaagaattc tcaaattgga aaaagattag taataccaac gatatttcaa tttattttgc
     1021 ggatccagga acaccttctc aacgaggatt gaatgaacat tcaaatggct tattacgtaa
     1081 agatggtttg tataaagcta tggattttaa tggtgtatct gaaaccttta tccaatcggt
     1141 ggcatcgaaa cgaaatcata ttcctagaaa atcattacac tatcggacac caatggaagt
     1201 atttttgagt tacgtcacaa aagatgattt gtctaactta atttgacaaa cgaaatttaa
     1261 taaatttcct tcaaaaatgc ctggagaaac tacaatgatc accgtatctt tttgtgcttg
     1321 ctccaacttc ttcaaatcta taatagctaa cagtgtccct tcttttacct cctggttact
     1381 ttcaacccgt atctgaaacg gacttccttc gagttcaact gtatctaaac ccatatggat
     1441 cagtattttt ctgccagatt cactttcgat cgttatggca tgtcgggtag gaaatatatt
     1501 atcgattatt cctttaactg gcgcatggat atatccatca tgattcgtta tagcgtatcc
     1561 aactcctatc atttttgaag aaaagacctc gtcttttact gctgttaatg gaataatttg
     1621 accactagct acagctttta gaatgtttct tgttttaaag aaatgcactt tttatcacct
     1681 ctactttaat gctggccaat attttttgtt tactttgatc atttcatcta gtattttttt
     1741 tgcgactctg gcatttggta cggtttgatt caatgtaaat gcttgtaaag ctttttgata
     1801 agaaccttca aagtaagcat ctactagtaa cttctctact gcaacttgtg cctccatcaa
     1861 tcctttatga aaatcactga ttggttttct tagtgtaatt ggctccacac cagttgcacc
     1921 aacatatgcc ggtatttcaa caactgcatc tgaccgtaga ttcggaatcg ccccttgatt
     1981 aggaacgatt agcataaagc gacgacgatc atcattcaac aaggaaatag ctatatcgac
     2041 gatatattgt ccatgctccc caaaattaaa gttcaatact tcttccacaa taccgcggcg
     2101 tattttatca gccatttctt tggtattttt ctctcgtcca tccatgacca tattagcacg
     2161 tgtatactct ttgtcagtat gctcaacgac catatcagga tataagtaat actctaagta
     2221 attattcggg atattacttg gaaaattttt cgtgatgact gacatcattt gaaaggtttt
     2281 ttgccaagtt ttatctccta tattaaaatc ggcgacttgc atttcttttg ttattagttt
     2341 ttcaataatc tctggcataa tttctcgttg caattcttta tcataaatcg atgtatacca
     2401 gccaaagtgg tttaaaccat aatacgtaac gatccaattc ttacgatcat aaccgtaatt
     2461 cactgcaatc gtttcttcaa ttgaaatagt catatcacag gcattaatca tttttacatt
     2521 cggatattta cgacgaacag cttcagaaac gatcgactct ggatttgtat aattcaaaat
     2581 ccatgctttg ggggcatagt cttgtatatg atcgacaatt tccaataaac ctttgattga
     2641 gcgcatccca taagcaaatc ctccgagtcc acacgtttct tgtcctacta atccatgttt
     2701 aagtggaatc ttttcgtcct tttctcgcat ctccaatcct ccaacacgaa tttgtgagaa
     2761 aacaaaatcg catcctgtaa aagcaagctt tggatcatcc gttgcacgaa tgcgaatatt
     2821 tgaaaaacct tttcttttca gcataaattt aacaatcaag tacatatcca tatttctttc
     2881 ttcatcaata tcatataaac gtatttcaga taatggcaac cgtttttcat tgttcaaaat
     2941 ggcttgaacg attcctggtg tatacgtact cccgcctcca gctatagtga taatctgttt
     3001 tttcatcatc atcttttcct ttctattctt ctaataattt attgaattct gccataacat
     3061 aagtcacttc taagccgatg ataatttgat aatttttttc agttggttta atgatcccgc
     3121 ttatccctaa ttgcttgatt cgctgttcct cgaccatggt gggatccact acctctaccc
     3181 gtaggcgtgt attacaacta tagacatcca ggatattggc ttttcctcca aacgcttgta
     3241 gcatttttgt tgccatatat tcataatttt tacttgctaa ccctaactct gtttcttcta
     3301 aggttacttc attgccaaac tctgccttag cttcacgtcc taatgtctga atgtttctct
     3361 tattgatcaa ataataaaaa gtaacgtaat acaaggcaaa aaagaaaatt ccgattggaa
     3421 gaatcaaaat agcattggta gcgattcgaa aattgatgag ataatcaaca atacatgctc
     3481 cgaatgtaaa accaattcgt atattgaata agtaacagac aatccctgct aaaccggcaa
     3541 aaagtgcatg gatcacgtac aacataggag aaagaaacat gaaactaaat tcaacaggct
     3601 cagtgatatt tgcaataaat gaagttcctg caccgcttcc catgattcct ttgaccatct
     3661 ctttattttc tttataagct gctttataga tagctagaca tatagctggt ataccaaaca
     3721 tcattactac aaaaaaaact gataaaaaca gtccagcctg aggatctccg ttgataaagc
     3781 gtgtcatctc tcctctgaaa acttccttgc taggagatgt atagcttcct aaatcgtaat
     3841 aaatatacgt attcaacaca tggtgcagtc caaaggggat cagcaaacga ttcaaaaaca
     3901 taaatatccc cacacctaat gctcccatat caacgagcag ttttgcaaaa ttatcaatcc
     3961 caatttggat gagcggccag ataaagccca aaaaaacagc tgcgattgtt tgtagaatca
     4021 tcaccatagt caatgggaat ttttcgcccg caaaataaga aaaaactact ggtaattttt
     4081 gttctttaaa gcgattatac gtccatgccg ctactaaacc ggaaataatt ccaccaaaaa
     4141 tccccatatt cacttctggg tccataattt ctaagccttt tttcaaggta gcaatcgcta
     4201 aaaaaccagt aagtgcaggt attcctttgt ccttattttt gacaaaaccg attgcaactc
     4261 ctatagcaaa taagatatcc aagtttccaa aaacggcatt tccagctgca ccaattactg
     4321 ggatgttcag catatcgtca gctgataaac gcatgatcaa tcccgctaat ggcatcgctg
     4381 caactggaag tagcatcgct cttccaagct tctgcacatt tgatttaaca ttcattttta
     4441 ttcctcctct attaaatatc ctcatatgtt actaactcaa tcaaaggatc atttttcact
     4501 ttttgagcaa atgctgaatg aattaaaaat tcgacttggt gggtacgagg atacacaaaa
     4561 cttgaaccat ttaacaattc ctgatccaaa tacgctgcgt gacacatgac ttcgatcgtt
     4621 ttatcttctc tcaatatcat gagtaaactt gccaaatatt cttccacact cgtattgatt
     4681 tgtcgttttt tttcatcagt ttctcctacg acatcgaaac aaggctcaaa ataatcgaca
     4741 tgacgaactt catcttttcg ttcaaagttg ctccgaaccg gcaaatcata tttttttgct
     4801 aatgtaacaa ctacctcctg attttcatcc aaagtatgag tatgatgatg actgtctaaa
     4861 tgagttggta tgatccccgc atgataaatt ttttgaatct gagcattcca ttcctgatac
     4921 agttgatctt tgtcaatgtt aaatggatgc tgataataag ataaggatct gaactctccg
     4981 ctttcgataa gagtatcgac agttttgagg agtggtttgc cacacgtcaa tgttaaatgg
     5041 actcctacac ctagcctagg catttccttt cttagtttca cagcatgctc gaaacccggc
     5101 atgatagtta cgcccttatt ttaatagatt cttgctagat gaattgccaa atagatgatt
     5161 tcattttctt gaatggcaag ttgaaattgc tgttcgatga tatctttaat ctttagtgct
     5221 gtacaatacg ctttgctatg tttaaatgtt tcagtaaaaa aaccaatatt ttctactggc
     5281 gtttcttcct gataaatcaa gcgtttaacc aggaatttga tatgaaccat taagcgatga
     5341 taagtcagtg attctttatc ataaacgttt gaataggatt tttctagatg ggacaaaatc
     5401 gtggtcgtca tttctactat ttttaagctc tttaaactat taggctctcc tagctcagca
     5461 ttaatgatat gcatagtgat gaatcctact tcatttgacc ctaactcggt ttgatatcga
     5521 tgattgattc gctcgaccac ttctactgct atatcatatt catttggata aaggaatctc
     5581 atttctggca acataattgc tgtgacatct ctatcttctc tttttcgttg aatagcaaaa
     5641 taaatatgat ctgtcaatgt aatatagata tttgaatcta atggagagga gatcctagct
     5701 gttatcagtt caatcacttc ttccgcaaaa tccataaatt cgattggaat ctgcgagact
     5761 atctcactga atcgtttgat cttttcttta gaatctaata caaataattt gtcagcatcc
     5821 tcaacatcaa atggcttact cactttagca gaaaagccaa tccctttccc catcgcaatt
     5881 ttttctattc catcgtcatc gatcaataca gcattatgat tcagcttttt
//
LOCUS       DAIGOK010000130         5902 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.131, whole genome shotgun sequence.
ACCESSION   DAIGOK010000130 DAIGOK010000000
VERSION     DAIGOK010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.131"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(49..918)
                     /locus_tag="NOA01_002313"
     CDS             complement(49..918)
                     /locus_tag="NOA01_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HCI0633479.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(1210..2820)
                     /locus_tag="NOA01_002314"
     CDS             complement(1210..2820)
                     /locus_tag="NOA01_002314"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HCI0633480.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(4013..5353)
                     /gene="glnA"
                     /locus_tag="NOA01_002315"
     CDS             complement(4013..5353)
                     /gene="glnA"
                     /locus_tag="NOA01_002315"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HCI0633481.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(5381..5773)
                     /locus_tag="NOA01_002316"
     CDS             complement(5381..5773)
                     /locus_tag="NOA01_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCI0633482.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         1733 a         1268 c          973 g         1928 t
ORIGIN      
        1 tcaaaagtcg ttctccattc ctatatatcg tatctgttat gtggatgctt acgctttgtt
       61 agctgaaccg aaccagtcga tatgagattt agcatcttca acgattgctg ctttacctgg
      121 agctaataat ttacgagggt cgaatccttt accttcttta tctttaccag cttcgatata
      181 tttacgagta gctgcagcaa atgataattg gaattcagtg ttaacattta cttttgagat
      241 acccattgag atagcttttt cgatttgatc ttgaggaata cctgatccgc catgtaatac
      301 taaaggcatg tctggaccta cagcatcagc aattgcttgt aagtggtcaa aagctaatcc
      361 tttccagttt tctggatatt gaccatggat gttaccgata ccgcaagcta agtagtcgat
      421 acctgtagca accatttgtt tgcattcttc aatatcagct aattcgccgt taccgatgat
      481 tccgtcttct tctccgccga ttgaaccaac ttcacattca actgagatac cttttgcatg
      541 tgctttttca acaacgtctt tagctaattt caagttttct tcaaatggta gatgtgaacc
      601 atcgaacatt actgaagtat agcctaattc gatacattcc aaagcagctt cgtaatcacc
      661 atggtctaag tggatagcaa ctggaacagt gatgttcatt gcttcaacta aatcagtgat
      721 taaatcttta cagattttgt aaccacccat gtatttagct gcacccattg atgtttggat
      781 aagtactggt gcttgttttg cttcagccgc ttcaaggatt gcttgagtcc attccaagtt
      841 gtttgtgttg aatccgccga cagcgtaacc gcctttacga gcagctttta aaaattcagc
      901 tcctgatact actggcataa taaaattcct cctaagtttt gtaaaactaa atttgttaag
      961 acaattctta accaacgacc ctattttacc aaacatttcg gtagttttct actaaaatca
     1021 atgaaaatga aaattttcaa gaagtttttt gcaaaaaata aaatatccaa tagcgcttta
     1081 atcgaaaaaa gtcttttgct ttctatcttt tttcctacaa ataataacaa atcagaaaaa
     1141 gagaaaagtg acaagaaaat tgcttatccc cttctcttgc tacttttctc tttctatcaa
     1201 gttcaaaaat tattttgttt cgtgatctgc taatgatgct ccgacaaatg ctttgattaa
     1261 tttttgcggg cgatttggac gagaaatcaa ttctgggtgg aattggcatc ccacaaagaa
     1321 ttttttctct ggcagctcta cgatttctac taaacggtta tctggtgaga cacctgaaaa
     1381 gactagccct tgttcttcaa acaattgacg atatttgtta ttgaactcat agcggtgacg
     1441 gtgacgttct tgcacaacat cggctccatc atatgcttca gccgtcttcg ttcctttttt
     1501 gattttacat gggtataatc caaggcgtaa agttccaccc aaattctcga tgttttcctg
     1561 atcagccatt aaatcaataa tattgtttgc tgtatctgga ttcgtttcag ctgatgacgc
     1621 atcttctaat ccaacgacgt tacgtgcaaa ttcgacacaa gccatctgca taccaagaca
     1681 aatacctaaa aacggaacgt cattttcgcg agcataacga atcgcttcga ttttcccttc
     1741 tactccgcga tcaccaaatc caccaggaac caagattcca tctgcgtctt tcaaacgttc
     1801 ttctacattt tctctcgtta attcttgtgc tttgatccaa tcgatctcga tgtctgaatt
     1861 ataggcgaaa ccagaatgtt tcaatgcttc aactactgag atatatgcat ctggtaactc
     1921 gacatattta ccgacaagcg caatccgtac ttttttcttc aagttcaaga cacgttcttc
     1981 taattctttc cattctgtca tgtctgcttc tggtgtatct agttttaaat ggtcgcaaac
     2041 aatttgatcc attccttgtg cttgtaaaac taatgggatg gagtaaagtg tatctacatc
     2101 tggcgactcg ataactgctt ctggttcgac atcacaaaat tgtgccaatt tgtttttcac
     2161 gctttgagaa acaggttgct ctgtacgtac caccaaaata ttcggttgga tacctaaacc
     2221 acgtagctct tttacactgt gctgcgttgg tttagttttc atttctccag cagctttcaa
     2281 gtaaggaatc aatgttgtat ggatatacat aacattatct gtaccgacat ctgctttcat
     2341 ttgacgaagt gcttcaagga atggcaaaga ttctatatcc cctactgttc cccctacttc
     2401 tgtaatgatc acatctgaat cagtcatttt agctgctcgc atgatttttt ctttgatctc
     2461 atttgtaata tgaggaataa cttgaacagt tgctcccaag tattcgccct tacgttcttt
     2521 acgcaaaact tcagaataga tttttcctgt cgtcacgttg gagtatttgt tcaaattgat
     2581 gtcaataaaa cgttcgtagt ggcccagatc caaatctgtt tcagcaccat catctgttac
     2641 gaatacttct ccgtgctgat atgggctcat cgtccccgga tccacgttga tgtatggatc
     2701 aaatttttgg atagttactt ttaagccgcg gtttttcaat aagcgtccta aagaagctgc
     2761 aacaatccct tttccgattg atgaaacgac accgccggtc acaaaaatat attttgtcat
     2821 aagttcaaaa actccttttt aatttatgtg caggaaaagt ctcagacaat aaacaaagct
     2881 ccctattctt caataagaat agggagccga aatttctgta aacttctgac cctctaaaag
     2941 ggtgcccaag gtgtataata caagctgact aagggaaagt caagtactca aatgaatact
     3001 atccgcttac aattttttct ttttcgatat cctttgagaa attatttctt aagtccttta
     3061 tgagtgcaaa aaaaatttaa cccacataat aagcttgata taaacatagc taacgttaaa
     3121 cctaagcaaa tcagaaaatc gttactgaaa aattttaagt gaaataatag ataaaaaaat
     3181 aaaacaaaca ttatgataga taatatccca cctaacaaag aactactttt tcctacttgc
     3241 agcaacagtt tatatagctt atcgtagaaa caatcttttt ctttcaccct tatttacctc
     3301 ctttacgtaa aaaacgtagt ccacagttct cggatttttg gcatatcttt tctattaaat
     3361 aatatcaaaa acaaaacaat tccactaatt ttttcaaaga tgaccttttt ttcaacaaaa
     3421 gtgccttgta gggattcatt gaaggtaacg aatcacataa gtaaaaaata aggagatgcg
     3481 acaaaagtcg tgcctcctta tttctaaata aacggtgttc tcctgccaac tcctcggtca
     3541 caagtcacaa tatccatcaa acatgagaag ctgtttttga aattatttct tgtgattcgg
     3601 agttaaatgg taggctcaca acctctggat aaacggtatt caaaagccag tttctcggta
     3661 ttaagccaaa tttatgcaaa aatgtagaaa gccattttca caaaattttt cttaatactc
     3721 gaaactaaac gtctttttca aaacctcttg gtaaacggtg ttcaaaagct tgcatctgcg
     3781 gtaataagtt cagccaattc aaaaacaaga aaagctgttt ttgcttggct attcttatta
     3841 cttgttgcag ggcagctttt tcacaacctc tccactaaac ggtgttcaat cagctgaccc
     3901 tcggtattag ctcaaaaaac ggcaaaatat gaggaactat ttttccgtta gtttctctaa
     3961 tactcaggtc aaaacgctga tttcacagcc tctaattcac ggtattcctg ttttaataaa
     4021 gttctagata ttgttctctt tcccactcgg aaacagtttg tctgaatgcc gcccattcga
     4081 gacgttttgc ttcaacaaaa tttgagaaga tatgttgtcc taatgcgtca atcattacat
     4141 tatccttacg caattctttg atagcattat ggatagttga aggtaagtcc tggatatgtg
     4201 cagcttctct ttcttcctca tccattacat aaatgttacg gtcaacagct gctggcggtg
     4261 tgatctgatt acggataccg tctagtcctg cttgcaacaa tactgccatt gccaagtatg
     4321 ggtttgcaga tggatcaact gaccgcaatt ccaaacgtgt ggataaaccg cgagattctg
     4381 gtacacgtac taatggcgaa cgattacggc cactccatgc gacataaaca ggcgcttcat
     4441 aacctggaac gagacgttta taagaattga ctgttggatt gcaaacggct gtgtaggcac
     4501 gagcatgttt cagcaaacca cctaagaaat gataagctgt ttcacttagt tccaattcac
     4561 cgtctttatc atagaaaaca tttccttcat cattgaacaa agacatattg caatgcatac
     4621 cagaaccatt gatcccaaat aacggtttcg gcatgaaagt cgcgtgaagt ccatgcttgc
     4681 gtgcaatcgt tttgacaacg agcttgaagg tttggatatt atcacaagca tctaccacat
     4741 ctgcatattt aaagtcaatt tcatgctgtc ctggcgctac ttcatgatgt gaagcttcca
     4801 cttcaaagcc tagactttct aattccaaca cgatgtcacg gcggcaattt tcacctaaat
     4861 ctgttggtgc aaaatcaaaa taaccacctt tatcattcaa ggtcgttgtg atcccaccgt
     4921 cttcgtctaa tttaaataag aagaattctg gttcaggtcc taaattgaaa gaggtaaaac
     4981 caaggtcgcg catatctttc aatgctcgtt tcaaattccc gcgaggatcg ccggcaaatg
     5041 gtgtcccatc cggattatag atatcacaaa ttaaacgagc aacttttcca tgagcacttt
     5101 cccacgggaa gatcatccaa gtggagagat ctggatacaa atacatatcg ctttcttcaa
     5161 ttcgaacaaa tccttcaatc gaagaaccgt caaacatcat tttattttct aatactttgt
     5221 cgatctggct cactggaact tctacatttt tgatcgtacc taagatgtct gtgaacatca
     5281 atcgtaaaaa tcgtacgttt tcttcctcaa tgattcgttt gatttcttca cctgttatct
     5341 gtttctttac catggtatcc ctcttccttt tttaatttat tcagtcaaag aattgtcttt
     5401 gcaatccttg cgactgataa tgacctgttt tgcttaaccc accttgcgcc aaaatctcat
     5461 cacgaaaaat ttgtcgaacg tcatgatctg tcaacggctt cttgtttttt gcttcttgtt
     5521 ttttcgcctg tgccatttcg tatacacgtt tgatcccagc catattcaat ccctctgaaa
     5581 gatagtcttt aatctcaagc aacatatcaa tatcattcag cgaatacata cgtcggtttc
     5641 cttcgcttcg ttccggatga actaaatctt gttcttcata atagcgaatt tgccgagcag
     5701 acaaatcagt cagcttcata actgtaccaa ttggaaatac ggacatagat cggcgcaatt
     5761 ccttttctcc catcagacgt cccctttcgt tttataagtt ggctaaagtg taccaattaa
     5821 aaaaaatatt gtcaactgca tatgttaggt tttctaacat gagatctcta ttttttataa
     5881 aataacaaaa aaataagaac ac
//
LOCUS       DAIGOK010000131         5858 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.132, whole genome shotgun sequence.
ACCESSION   DAIGOK010000131 DAIGOK010000000
VERSION     DAIGOK010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.132"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(461..1090)
                     /gene="udk"
                     /locus_tag="NOA01_002317"
     CDS             complement(461..1090)
                     /gene="udk"
                     /locus_tag="NOA01_002317"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCI0633483.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1174..1869)
                     /locus_tag="NOA01_002318"
     CDS             complement(1174..1869)
                     /locus_tag="NOA01_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HCI0633484.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1882..2598)
                     /locus_tag="NOA01_002319"
     CDS             complement(1882..2598)
                     /locus_tag="NOA01_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HCI0633485.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2928..3533
                     /locus_tag="NOA01_002320"
     CDS             2928..3533
                     /locus_tag="NOA01_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HCI0633486.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLCEHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3858..4181
                     /locus_tag="NOA01_002321"
     CDS             3858..4181
                     /locus_tag="NOA01_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HCI0633487.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4363..5733
                     /locus_tag="NOA01_002322"
     CDS             4363..5733
                     /locus_tag="NOA01_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HCI0633488.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
BASE COUNT         1666 a         1154 c         1096 g         1942 t
ORIGIN      
        1 tagggggtgc gacaaaagag cttcttcaaa aaaataagtc gaaatctcac acaaattcga
       61 gaaataattt tcgtaagttt cttcttattt attgcatctg accacttctg ttacaacctc
      121 tcgttaaacg gtgttcaaaa gcttgcatct acgataataa gcccaaccca acgaaaaacg
      181 tgaggagctg ttttatttgg atgctcttat cgcttgatgc aaagacagct tttttacaac
      241 ctctacttca cggtgctcaa tcagctgacc ttcggtatta gctcaaaaaa cggtaaaata
      301 taaacagcta ttttccgttt cttcttttta atacttaggt caagatgctg ttttcacaac
      361 ctcttgttat aaataaaaaa acagttggct ggtgactgtt caaaaagcag aatccttgct
      421 tttcaaacgg tcgcagtccg ttcaactgtt tcgctgcgca ctatttatgg tttaagaaac
      481 tagctacttt tgttgtaatc aaatcaatag caacgtggtt ttctccgcct tctggaacaa
      541 tgatatccgc atagcgcttc gttggttcga taaattgatg atacatcggt ttaacaactg
      601 tcaaatattg ttcgatcacg gagtccaatg tgcgtccccg ttcttccata tcccgtttga
      661 tacgacgaat gatacgaatg tcatcatctg tatctacgta tactttgata tccatcaagt
      721 cacgcagacg ttcgtcttct aaaatcagga ttccttctag aatgatcact tcttttggtt
      781 cctgaatgat cgttgcctga cttcttgtat gcgctacata gtcatacacg ggtttttcaa
      841 tggctttata gttcagtaag tcgcctacat gctgaatcag cagatcatta tcaaaggcaa
      901 atgggtgatc gtaatttgtg ttcaatcgtt cttcaaaact caaatggctc tgatccttgt
      961 aataagaatc ttgttcaagc atcatgatgg aatggtccgg gaagttgttg aaaatcgcac
     1021 ggctgacact tgtctttccg cttccggatc cacccgtcac accaatgata attggcttgc
     1081 tttttgtcat tgactaaaac ctctttcgta tccgtttttt cattctaatc tattatagcg
     1141 aaaaaacacg aaaatgctat atttttctga aaattaatcc ttttcaattt ccaataatga
     1201 ataaagttcc tctaatgaat cgattcgata agtaggctgg atttcttcga tttctggtaa
     1261 tttatttcta ttgaaccaaa tcgtatcgat ccctgccata tttcctcctt tgatatcaga
     1321 agtcaaagaa tcaccaatga tcacagtttg ctttttatca aaattaggaa tgtgggaaaa
     1381 gacatgatca aaaaattctt tcatcggctt ttgcgctccg acagcttctg agacaaagat
     1441 atccttgaag taatcaagca ttcctgcatc tgttaatcgc cgatgttgcg tgtgcgacac
     1501 accgttcgtc acaatataaa gatcagcatg cggatggacc tttttcagga tttctagact
     1561 tcctggaaca agatcatgac cttggttcaa gtagtggcgg tagcgttttt ccatttgatc
     1621 actatctact gttttcccaa attgctggaa aagtaaccca aaacgactgc ctattatttc
     1681 ttctcgtgat agtctgcctt tttcaaactc ctgccataaa tgatgattca aagccttata
     1741 gctggtttcg atttctggtg tcaatgtgat tccttgttct gcaaacaaag cacttaaagc
     1801 attggcttca gttaattgaa aatctaacaa tgtatcatct acatcgaaca ataaagtttt
     1861 atatttcatc tttttgacct cctatttaaa ataaatactt aactttttca tctctgctaa
     1921 tgtcaacgat cgatactcgc ctactcctag ctgttcatct aattccaatg gtcccatgct
     1981 tagccgtttc aatgcggtca ctttctttcc ttgggctaag aacatctttt tgacttgatg
     2041 gaattttcct tcttggatcg tcaataaaac cgtactttgg tgcttgtctg ctttgagtat
     2101 ttttaaatca gccggcttac attgataccc atcagcgaat acgattccat tttcaaatgc
     2161 tgttacgtct gccggtgtca ccctttcgtt cacaactact tgataagtct ttgaaacctt
     2221 tttttctgga agtagcattt catatcccaa ttgtccattc gaagtgatca gcaatagtcc
     2281 tgttgtatct ctatctagtc ttcccactgg atataatggt tggaaacgat cggcacttgc
     2341 aatcagatcg attactgtcg tcttttctgg atctgataca gctgtaacag ctttttcagg
     2401 tttattgaga agataatagg actcatttgt ttctaagtgt tcacctgcta cttcgatttg
     2461 atgcagtaaa ctatctacat ttcgattttg tcgaaattcg actgtgccat ctatcatcac
     2521 ttgcttcaaa gcaaacagcc gcttcatttc cttgcgtgtc gtttctaatt tttcttctat
     2581 taatttatct aaacgcatgc tgtttccttt cttattacta ctaattaatt ataccttgaa
     2641 tattcttgct tgcaaataca tcatcatagt tattccttga cttaattaac agttcacgtt
     2701 acggttaaat agtaaagtga attggatttc tcatacacag cgtaatgacc tggtggagaa
     2761 aagttgaatc ctgactttct aaggggagat gcgcgcatct ctccttttgc gcttttttaa
     2821 gcgccctgcc ttcgatgatg tgcacgagat tagcctttag aattcactta aataaaatct
     2881 gtccgttacc aaagcagaca gtcacatttt aaggaggaat tttggttatg tcagtatcat
     2941 tagaagtaag taaaagagaa gtacgtcccc gttcattaag aaacaaactt cgtcacgaag
     3001 ggaaagttcc agctgttgtt tatgggtata aagtagaaag tacccctatc tcattcgatg
     3061 gtttgacact aagcaaattg ttgtgtgaac atggagcgaa cacagtcatc acgatggaca
     3121 tcgatggaca aaaagtcaat acattggttc acaagatcca aacgaacact tttaccaatc
     3181 atttcgaaca tgcagaattt ctatctgtaa acatgtcgga agaaacagaa gtcgaaacag
     3241 aagtcgttct tgttggcgaa tcagtgggtg ctaaatctgg tggagtgtta gctcaaaact
     3301 tgtatacagt agtcgtatct gctacaccag acaaattgcc tgaacgaatt gaagttgacg
     3361 tgacaaactt ggaattaggt gattctatta cagtagccga tcttccaaaa aacgacgatt
     3421 acaaattcgt gaccgacagt gaagagcaaa tcgctgctgt agtcgctcaa agtgaagaag
     3481 aaagcacagg tgaaggtggc gaaccagaag tgatcggttc aacagaagaa taatactgaa
     3541 acaatcaggt ggtgttagaa gcggcgggtc agcagctttt aacacgcctt ttttatttct
     3601 aatcgtttat tttcatatgg ggcttccact tttgtaaatg gctttacaaa cgaaaaatcc
     3661 taatttacca agcttttcct cgcatacttt tatcgtacct ttataaaaat ttatactttg
     3721 aaaaaaagta ttcaattatt gttgcatttt tcctgattct ttattagaat gaaccctcgt
     3781 tgaagtaagt atttctagtt tatcctagga ttaattataa aaataaaaca ttcgttgatt
     3841 cgaaggaggt taaacagatg tttgaaagtt ttgatagcag ccgcaaccgt tacgcttcag
     3901 ttggtgtcgt atcaagtttg ccagatgaat tgattgacag tatatggttt attattgacc
     3961 tagatcttaa gggagtcatt ccattagaaa atatattaac ctttgatttg atcgaaaatc
     4021 gtggaaaagt gacgatgcat ttttctcaag caggaagcaa tgtggaaatg gatattgatt
     4081 tgccgtttgc ctattcgaac caatttcctg ctcaagtata cgcctatgat gacggtacga
     4141 gagaaacgat ccttttacca agtgagatta gacaatatta aggtgataga taaacgtgga
     4201 tgtcccttat tttacagacc aggccaagtt gttgattctt tgactcaatt cttggcttct
     4261 ttttatggag tttcataaat tatcattgat ttctaattgg tattctcagg ataaacgcta
     4321 tatactacta actaaatttt aaagatataa ggagtttaaa agatgcgcga tttaacaaaa
     4381 ggaagccccg ctaaactgat tttgatgttc actgttcctt tactgatcgg aaatgttttt
     4441 cagcagttct acaatatgat cgatatgatc atcgtgggtc agacgctagg taaaaatgcg
     4501 ctggcagcgg ttggtgcaac tggaagcctg acatttttga tcattggctt tgcccaaggt
     4561 ttaacagctg gtttagccat tattaccgca caacgttacg gtgcaaaaga ttatcggggt
     4621 ctgaagaaaa gttttgctgc tagtgttgtc attagtcttg tcgtcactgt gattttaact
     4681 gtcctaagtc tcctgtttat tcgtccgatg cttcaattga tgcaaactcc tcctgaaata
     4741 cttgatcaag cacaaacatt tatttcaatt attttattag ggatctttgc atcagtcagt
     4801 ttcaatttgc tttctaatgt cattcgcgca cttggtgaca gccgcacacc actattcttt
     4861 ctgatcattg cggtcatcat caacgtcgta cttgatttga tctttattat ctattttggc
     4921 atgggtgtgg aaggtgcagc tattgctaca gtaatcgcac aagtttcttc cagcgtactg
     4981 tgtctagtgt atatcaagaa aaaaatgcct ttgttacagt tacgcaaaaa agatttcaaa
     5041 tttgaaaaag aagagtttgc tgtacaccta aatgctgctt tgccaatggc gtttcaatct
     5101 tcgatcatcg ctgttggtgc tatcgtgtta caagcagcac taaatagtct agggacagat
     5161 gtcgtagctg ctcaagccgc tgctggtcgt atcgaccaat ttgcaaatca gccgatgatg
     5221 tcatttggta ttgcaatggc aactttcact gcacaaaact atggtgcgaa agaatatggc
     5281 cgaatcttgc aaggggtacg tcaaacttta ctgatgagtg tcggatttag tctcttagca
     5341 ggtgctatcg tcattttctt cggtcatttc tttgttcaat tgtttgttag cccatcagaa
     5401 acacgtgtat ttgaattagc acaaacctac ttcaatatca atgggtctct ttactggatc
     5461 ttagcagtcc tgttcatttt acgctacaca cttcaagggc ttggacaaag caagatccct
     5521 acgatcgccg gtatgatgga attgcttatg cgttcttttg cagctatcgt tcttacagga
     5581 atgtggggat acccaggagc agcagcagct tctcctttag cttggatcgg ttctgttgcc
     5641 gtgttgcttt actcttatat gcgttcgatg aaacaattga aacacatggg tgaagaggaa
     5701 catttcacat tagaacatgc agaagcaaga tagccttgat taaaagaaat cgttgcggct
     5761 aaaaatgaaa acttttattg aaaatatctt cttcgtttac cgaaaacagg ttttttagat
     5821 aacaaaaagg ctgtgacaaa aaatgtcaca gccttttt
//
LOCUS       DAIGOK010000132         5760 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.133, whole genome shotgun sequence.
ACCESSION   DAIGOK010000132 DAIGOK010000000
VERSION     DAIGOK010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.133"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(28..507)
                     /gene="rlmH"
                     /locus_tag="NOA01_002323"
     CDS             complement(28..507)
                     /gene="rlmH"
                     /locus_tag="NOA01_002323"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCI0633489.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            936..2225
                     /locus_tag="NOA01_002324"
     CDS             936..2225
                     /locus_tag="NOA01_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCI0633490.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(2264..2869)
                     /locus_tag="NOA01_002325"
     CDS             complement(2264..2869)
                     /locus_tag="NOA01_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HCI0633491.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(3076..3540)
                     /locus_tag="NOA01_002326"
     CDS             complement(3076..3540)
                     /locus_tag="NOA01_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCI0633492.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(3562..3882)
                     /locus_tag="NOA01_002327"
     CDS             complement(3562..3882)
                     /locus_tag="NOA01_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCI0633493.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTASWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            complement(4025..5104)
                     /gene="pepA"
                     /locus_tag="NOA01_002328"
     CDS             complement(4025..5104)
                     /gene="pepA"
                     /locus_tag="NOA01_002328"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HCI0633494.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            5304..5654
                     /locus_tag="NOA01_002329"
     CDS             5304..5654
                     /locus_tag="NOA01_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCI0633495.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
BASE COUNT         1678 a         1156 c         1080 g         1846 t
ORIGIN      
        1 ggataatttt tctcttaaaa aatatcatta tttgtggtat ggagcccccg cattgatccg
       61 aaaggcacgg tagatttgct cgacgaggat cagacgcatc aattgatgtg gatacgtcat
      121 tttgccaaaa gagatctgag cattgcttct tttcatgacg gcttcgctga gtcctaaaga
      181 gccgccgata acaaaaacaa ggtggctttt tccttggata cctaattggt cgatttgttt
      241 agcaaaagat tcgcttgtgg gatttttccc ttcaattgct aaggcataga catattcttg
      301 ttctttgatt ttcgctaaga tccgttctcc ttctttttct tttacttgtc tcatttgcgc
      361 ttcacttaaa ttctccgggg ctttttcgtc aggaacttcg atcaattcga tttttgcata
      421 ggcattcagc cgtttgacat actcattgat accttggatc aagtattttt cttttaattt
      481 tcctactgaa ataattttga tattcatgtt ttctctcctt tggtcttcat gctcactttg
      541 tttattataa tcaatttttt cgtctatatg tttattttgg ctatttttgt taaaaagatt
      601 tccccaacag ttatgcacaa catccacagg tttatccaca gaaaacagtg ttattttacc
      661 ttatttcatc acattcatta ggcaaacata cgttttataa gaatttatcc actttataca
      721 caggctgtgg atatgtttta tcccttattt ttccaatcct atccgcgtag ttttacacag
      781 tttctttgga aaaaagcaaa tcaaatattt tcatctgaaa acaatggttt ttcctaaagt
      841 ttttcataaa aagttcacag tctcaaagta gactaaagtc aacaaataag agtatactaa
      901 cagtagataa atgaatgtaa ttggaggagg aacacatgga tagaaaaaat gtgacaccaa
      961 agatgaaaaa aaataaaaat aatagcctct ggcgtaagtt aggtctgggg cttgttggag
     1021 ggatcgttgg aggactactg acagctggca ttttttatgc agcgatgggc actggcaaca
     1081 atagttctac tgctacaagc ggcaatcaga attctgccgg tgaaacagtt gtggaaaatg
     1141 tcaaagtcaa tgtagattct gatatcacca aagcggtaga taaagttcaa gacgctgtcg
     1201 tttctgttat caacttacaa agtcaaaacc aatcaagcgg ttttggcgga ctattcgggc
     1261 agcaagaaga aagtagcagc tcagatgata gtaatttgga agcttatagt gaaggaagcg
     1321 gcgtgatcta caaaaaagac gggaatactg cttatgtagt cacgaataat cacgtagtag
     1381 atggccagca aggtcttgaa gtcttgatga aagacggtac gaaggtaaaa gctgaattag
     1441 ttggaacaga tgcttattca gatttagcgg tgttgaagat caattcagac aaagtcgaaa
     1501 cagttgcctc ttttggtgac tcaagtgctc tgaaggttgg tgagcctgcg attgcgattg
     1561 gttccccact aggatctgaa tatgcaaatt ctgtcacttc aggaatcatt tcttccttga
     1621 accgacaagt gacaagtacg aatgaatcga acgaaacagt caatatcaat gcgatccaaa
     1681 cagatgcagc aatcaaccct ggaaattctg gtggtccttt agttaatatc gaaggacaag
     1741 ttatcggtat caactcaagt aagatcgcaa gtacttctga atctacttct aatgtaagcg
     1801 tagaaggaat gggatttgct attccaagta acgatgtagt gaatatcatc aatcaactgg
     1861 aaaaagacgg aaaagtgaca cgtccagctc taggtatcac aatggtcgac ttatctgccg
     1921 tttctactca gcaacaagaa caaatcttga agattccttc atctgtaact aacggtgtca
     1981 tcgtgacaaa tgtccaaaca gctacaccgg ctgaaaaagc tggattgaaa caatacgacg
     2041 tcattactaa gatcgatgac aaagaagttt cttctggtgt agaacttcaa tctgctttgt
     2101 ataagaaaaa agtgggagat tctgtcaaaa tcacttacta tgaaggctcg aaagaaaaaa
     2161 caactactgt tgaattgacg atcgatcaat cggcattaaa acaaaatcaa aatagtgaaa
     2221 attaataaaa aagaggttgt gacaaagact tgtcacaacc tctttatttt ggaaatgctt
     2281 ctccgactac ggcatcctca ggcaatacta agattccttt ttttgctgga gcatttggca
     2341 aatgcaattc acgagcggaa cagatcattc catagctatc tacccctcgc aaagttcctg
     2401 gccagatcat cattccatct ggcatcatag ctcctggttt tgcaacgaca actttttgtc
     2461 cagcttcgat attgggtgca ccgcaaacga tttgcaatac tgtaccattg tctacttctg
     2521 tctcagtaat cgataaatgg tcagaatcag gatgtgggac gcatgttttt acgtacccga
     2581 caacgatctt cggttcatga tcagcttcaa gctgttcttc gaatcctgct tctttgatca
     2641 atcgattcaa tttcgctaat tcctcatctg ttaagcttac ttggccgttc ccttcgattg
     2701 ttagatgatc ggagatatgg aaaaagttcc aagcaacaac ttgtccttgt tcatctttga
     2761 tccgagcaac attccatttg cgttcaacgg tgttttctgt tcctttatcg tcggctataa
     2821 taaccatcaa ggtgtctccg acgtgtgctt tgttataagc aaaaatcatg ttctttcctc
     2881 cattatcctt tgctgagatt tatcttaaca aaaaattgca ttcactgcat gagatttttt
     2941 tcatgcttta tttccgaaga agcctcttct ggaacaccat ttattcgtaa caagggactg
     3001 tgacaagact tttgtcacag tccctttact gttttttagt taattgatta aaaaggtcgt
     3061 acaagttcca cttgcttaat tacttggctg ttcttcttcg tcagaaacga tcaagacatc
     3121 acaaggtgaa tgacgtatca cgtagctggc tacacttcct gtcataaagc gttcgactgc
     3181 atttagacct gactgcccaa ccatgattag atcgattgca tatttttttg gcaatttgac
     3241 tgccatggat tcacgaacag aaccatatgc aataattcct tctatatttg tgtaatcaac
     3301 agatttccca tattctttac attcttcgat catttgcttg gctgtttcag tttgctgatc
     3361 gactagattt tgatcaatag gtgcatagcc cataaagtta taaaattgct gatcgattac
     3421 gtttgctaca tatactttcc cattattacg gcgagccact tctaccgctt ttttgaaagc
     3481 agcaagtgct tgttcactac cgtcgatacc tactaaaatg ttcttatatt cttgattcat
     3541 cttgacccct cctaacagat gtcagccttt tgttacactt tctaggaacg attcgatttc
     3601 ctgttttgtt ttacgatctt tattcactag acgacctaat tcttttccgt cttggatcac
     3661 gacaaaactt gggataccaa aaatgttcca ttcagcggct acatctatgt acttgtcacg
     3721 gtctacttca ataaattgcc aatcagaaaa gtcattttca atttcaggca tttgcggttt
     3781 gataaaacgg cagtctccgc accagctggc agtaaagaaa aatacactct ttccagtttc
     3841 tacgtagcct gctaattctt ctaaactatt tgggataatc attctttgtc ctctactttc
     3901 tgtttatgta tttttcaatt aaattataca cctttctttc gaactaacaa acattgatcc
     3961 tctttttata aaaaaaatct ggcaaaagca atcttttgcc agattttata gtttcactat
     4021 cgcattattt tccgtatacg atcgtattca ctgttgattt atccaaacct ttcagtgttt
     4081 gaatcaacat ttctcgtgca gcttcaaaat cgcgaatact gaacatcgtt tgatgcgtat
     4141 ggatataacg tcccactacg ccaatcactg tacttgggat tccttcgttt tgtgtatgag
     4201 cagcacctgc atctgtaccg ccttttgaaa caaagtattg ataagggata ttgtttgttt
     4261 cagcgatatc cagcaagtac tcgcgcaaac gaggaagcat gatcaaacct ggatcttgga
     4321 tacgcatcaa tgtaccttct cctaaatgtc cgaatgtgcc atttttcgtc actgtatcat
     4381 ctgctgcaga gcagtctact gcaaagaaca gatctggctt gaatttattg acagaagctt
     4441 tagaaccacg caagcctact tcttcctgga cattcgcgcc tgcaatcaat gtatgaccta
     4501 atgtttcatt ttgcaacgct tctaaagctt ctaataccat tgtacagcca tagcggttgt
     4561 cccatgattt gctgatgata ttctttccgt tggctgtttt gatcgtttca gcgtaaggaa
     4621 cgattgtgtc tcctggaagg acaccgtaac tcattgcctc ttctcttgat tcaaacccag
     4681 catcgaacaa aatatcggtt acttctactg atttttgtcc gcttgttcct cgcaataaat
     4741 gcgggggaac ggaagaagaa atacatggat aattcttacc agtagaggtt ttaagtgtaa
     4801 aacgctgtgc agaaacaacg taaggattcc agccgcctag aggtacgact ttaaaaagcc
     4861 cgttttcttg gatttgtgtc agcatgaatc cgacttcatc catatgtgct gcgaccatta
     4921 cacggggtgc atccgcttct tcatgatggc gcagaccaaa aatgccgccc aaaccatcta
     4981 actgtaactc atctaccagc ggttccatat gactttccat ataagcgcga acatcttgtt
     5041 caaaaccgct tgttccttgt aattctgtta actctttgat tcgttgaaat gttttttctt
     5101 ccatatgcta ttcgtctaaa tttcagacta aactcctctc agacttatat tgatctgcgg
     5161 ataaaatatg attttttctg cagaccgatc gtatttatta tagcttattt tttctggtag
     5221 cggttattta tttcaaagaa aatatgagag tgtggtaaaa taagcacaag gagtaacaag
     5281 tgagaagaag gaggtatgtc cacatgtatg aagaaagcga actaagctat tttaaaggcg
     5341 ggttagcagt tggaatcggt ctaggattat tgagcggggt agcttcaacg atttggtatc
     5401 atcgcaaaaa agtattgtca gcagaccaag tacttgaaaa tatcaagtca tcttttctaa
     5461 aagaaggtgc aatcgaaggt tcttggatct cttttgaaaa ggaacctatt aggaaattcg
     5521 ccgttcggtc gagtgcttat cggggaggtc tttctcgaat agaagatggc gaaactgttt
     5581 attatgaatt tttagcagat gctcatacag gaaccgtatt agaaatttca agaaaaaaag
     5641 gtgcaacaga ttaatacgtc aaaaaagtga gagaacgaat acgcacccac tattcaaaag
     5701 agaggtatcc tatacttctt ttatagtggg taatttttat aaaaataggg ctatttcctc
//
LOCUS       DAIGOK010000133         5674 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.134, whole genome shotgun sequence.
ACCESSION   DAIGOK010000133 DAIGOK010000000
VERSION     DAIGOK010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.134"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(<1..430)
                     /locus_tag="NOA01_002330"
     CDS             complement(<1..430)
                     /locus_tag="NOA01_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HCI0633496.1"
                     /translation="MTYVFLPKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISV
                     FGDVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILAVATVGLYGIGDCI
                     FTGLFSINTNESSWNLSTWIHNTGSGLGYAGFLLFPLLLVL"
     gene            complement(488..1540)
                     /locus_tag="NOA01_002331"
     CDS             complement(488..1540)
                     /locus_tag="NOA01_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCI0633497.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(1553..2491)
                     /locus_tag="NOA01_002332"
     CDS             complement(1553..2491)
                     /locus_tag="NOA01_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCI0633498.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(2941..3591)
                     /locus_tag="NOA01_002333"
     CDS             complement(2941..3591)
                     /locus_tag="NOA01_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633499.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(3691..4200)
                     /locus_tag="NOA01_002334"
     CDS             complement(3691..4200)
                     /locus_tag="NOA01_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCI0633500.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(4517..5080)
                     /locus_tag="NOA01_002335"
     CDS             complement(4517..5080)
                     /locus_tag="NOA01_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCI0633501.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(5095..>5674)
                     /locus_tag="NOA01_002336"
     CDS             complement(5095..>5674)
                     /locus_tag="NOA01_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633502.1"
                     /translation="VAALRLIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQ
                     LVSGNFTLKGEGQLTFYLLPTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHY
                     LLASRSYGPYTSELLVSERDSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYRE
                     KANPVLANVLLLDLATLLHANKRPKKIAATKK"
BASE COUNT         1802 a         1170 c          853 g         1849 t
ORIGIN      
        1 gcagaacaag taacaatggg aaaagtagaa agcctgcata gcctagccca gaacctgtat
       61 tatggatcca agtcgataga ttccaagagc tttcattcgt attgatactg aataacccag
      121 taaaaataca atcacctatc ccatataaac caactgttgc aacagcaagt attgcaagtg
      181 tttttgatgt tccaacaaat aggtgataca acgcaggcaa cgaaagaaca aaaaaaagtc
      241 cagatacaac tgaccagacc aagaaagcac gtctgactgg actatctacg tcaccaaaca
      301 cactaattac ggtagtcatt tgattcagtt tgggataaaa aaggccaagg atatacggtg
      361 tcgataaatc actaatcaca cctaataata aaaaataaaa accatatttt ggcaaaaaaa
      421 cgtaagtcag atgttgttga catctcactt acgcatccat tgattgaaaa tttcttagta
      481 aaatctacta gcctaaaaat ttgaagactt cttctcgatc atacagttta aatactttct
      541 cgcataaaaa ttgagtaatt agtgccgcaa aaacagggat gactaaccaa caaagaacaa
      601 caagaaagcc atttagccct gcatctaaag aagccaacgg accaacaagt ccgaccacac
      661 cgaaaccagc agaagcaggc gtacctgaaa tagaaaatag agcgacagga atagctgtaa
      721 tcactgcact aactaaacaa ggaataagaa tcacaggttt tctaaataga ttaggcatca
      781 tcatcttcat agcgcctaaa gcaatcgcaa ttgtcacacc tgacttatta accttccaag
      841 agttcactac taaaacaaaa gtggttgcag ctacgcccat cgcagccgca ccagcagaaa
      901 caccatctag ttggattgcc attccgattg ctactgttga aataggagag ataatcaaaa
      961 aggcaaagga acaaccaatc aagatagaca ttaacagcgg ttgtaaggta gtgaagttat
     1021 taatcaatga tccaattgca gaagtaattg ctgttacaaa agggaataaa taaaaaccaa
     1081 gtaaacctgc acctacacct acaacaatcg gtgttaacac aatcgacact gacccaaaac
     1141 tatttccaat cagtaaaatc agcccgacag caatcgatgc tgtcagcatc gtattaataa
     1201 tatcgcctgt accagcacca acgtaaacac ccgtttcacc caaatcaggt gtgaatttga
     1261 ctacaccaga accaacaaaa gaagctccgg ctacaaccat cattctcatc ggatcaaatc
     1321 caaattgtaa ggcaatcaaa ccaccaatga tcaaaggtgt tgctaattga aaaataactg
     1381 ctaactgtgc gatcgttacc gcaaagggat acgcactaaa atattttaaa atagcaccta
     1441 atactgcatt aggaatcaat ccaataatga ttcctgttgc tgtaccagcg aggactttat
     1501 tcagaaaaat tttcggtgat attttttgtg aagtttccat tcaatctctc ctttatttta
     1561 cgttcaaact atcttctttt gcatggacaa gctcagtcaa gaaatcacaa taaggcgtcg
     1621 caacaccata tttcttccct tttctagaaa ttgcgccatt gatataatcg atttctgtta
     1681 atcgatggtt cttaatcaag tcttgataca ttgaaggata atgtaaccca attgtttccg
     1741 ggtcaaaaca agattcacaa tgtgcaatga cttctgggac atctagttca atcttctcta
     1801 ctgctgctac tttggcaaac tcattgacaa tcgtcgccac cattttatga gcagtcgatg
     1861 tttttcctag ctcagccata ttaacgtcta agattgtgca tagtccgttc attgttccgt
     1921 tcacacatgc tttgcgataa atagaatagt gaatattgtc agaaaaatga gcatttaatc
     1981 ctgattcaga caatttgtct gctaattttt ttgcagctgc ttcttttcca tcacctaaat
     2041 tttgtaattc tactgaacca cttccaaata atttgacctg accaggacct tcaagacctg
     2101 ccgtccacat ggtatttcca atatagatat tttccattgg tacaaatttc tcaataatat
     2161 cttcatgccc gataccattt aatagacata agacctctgt atcttttttg attaatgatt
     2221 ggatatcctg cagcattttt tccagctgca tcgctttggt aaatagaata atcagatcaa
     2281 cttgatcttc tttttctact tcggattgaa gaacgattgg tagttttgct tctacctctt
     2341 taccattaaa atttgcttgt aatccatgtt ctttgatttg ttggacatgt tctgcccagc
     2401 catcgattaa taatacttcg ttcccacttt ggtgaagcat taacccaaag cgacttccca
     2461 ttgcgccagc gcctgcaatt gctattttca ttgattcttc ctcctttttc tttctattat
     2521 acttatttga gaatcatttc acaaccttgt tttgttattc ttctcttatt tatcatgttt
     2581 atatccacca ttcaagttat tttatcagca aatcaataaa actggaatag aatcccaatt
     2641 tcatcgtaag agaaactaga ctagcaataa gaaaatagaa acattcatag aaaaaacctc
     2701 actcctctga ttataaaaga gggggcatta acctcgtgta tactaaaacc ttccattcat
     2761 tcgatggatg attttctttt tgtgaaaaaa tattcattgt agtacgatgt tgcgcaacac
     2821 agacggatgg tattgcttat tatttattaa aatcgaacaa tacacttatt caaaaacttc
     2881 tttcccagat tttcttccat ttttacagtt ataactagta ttatcatgaa aaatagcctt
     2941 ttattgtcta tgggaataat aatttaaaat tggtactttt ttattaatta gttcatcgcc
     3001 caataatctc tccagtttct tagcatctcc tgactcaacc ggaattatcc aagcccagtc
     3061 caaataattt tttaaaatct catcatattt tgcaaatatt tcattgccat attccgtcct
     3121 taaaattata ttttcagtgc cagacaagtg atttgtattg agttgataaa aaagatctcc
     3181 atctaaaaaa atatcattag aagaagattt ccccacagta atgaccattc catcagctct
     3241 atgtgttaca tacaaataat tgtatgacct catttcacct aatgaatcaa ttatttcttg
     3301 ccctttagct ctaatatatt gatctctatt acctatctca ctcttattta acaaaagttt
     3361 tttcatgtca aatttaatcg gctgttttat ttttgtaaat tcgcttctac tactaatcca
     3421 atccgagatt aatttttcaa catgtcccag agtaaactct gatttattcg ctaaatactt
     3481 tttttgttgt attaaagaca tctttccccg aagctcaaaa ttgagcctac atcgattctc
     3541 tatctcttca aaaagcatct tatcttcatc attcttatat tcatcattca taaaaccaat
     3601 ccagctcctc gattaagata atattttaat ctcatacaaa aactagctct catcaaaaca
     3661 accattgttt tgatttctca tgaaatttct ttatactttt ctccgattat aaattcctaa
     3721 ccccttgaga aaattacgca tatatttatc tccgcaaggc ttaaaatttc gttggccttc
     3781 ttttctcaaa aatgccccga tttcgccttt tgatggatag atttctgcct cagcaaaaac
     3841 gtctaaaata tcatcagtgg tcatagacaa ggcgattttt aattttttta gcaacacatt
     3901 gttgatatat ttgatttcct tgcttttttg cggcgtgtct actatcggct tatctttttg
     3961 cggaccacgt gccaagagaa tcaggccatt taggaatgcg tcaaaaactt ggttgttgat
     4021 ggttttttca tacacattat tttctgcatt atctttgttc ttatcctgta ctttggtcag
     4081 taccgcctga gcttcttctt tagtcagtgg tacaccgcct agctcaaaag ctttaatcaa
     4141 atccgtatct ttcaaatcaa tcgcataacg caatcgcaat aacctatcat tgttattcat
     4201 ttgttcctcc gaagtattta gtctttactt tcttacgtac tttttgtctt gctaagattt
     4261 ttaggatcaa tgataaaaac cgcatcactt atgataaggc gctgcgtatc atgagtgaat
     4321 gatattagga aaacttcata aaataaatct tttatacatt tccacttcta gatggtcatt
     4381 catgttagaa gaatcaaact atttttttac tgtcgtaata atattaaaac actagaacaa
     4441 atcaattaaa ttccataaaa tataaataat agacctagca tcatctggta taaaatggat
     4501 gctaggtccg ttatatttac tcaaaattga ttctacgaat agatcttctt atcatttcta
     4561 ttttagtagt catgcgttgt tgttcttcta aaggtggtaa tggtataagt agtttagata
     4621 aatttgtacc atttacattt ggttgcccag ttccgtaaga aattttttct aataacttcc
     4681 aatataatgg cgagtctaag aagcagtcta cgtattctga taataatttc tcaactaacc
     4741 taattcgaat caagtaagaa gcaaaaacag attcctcact tatttcctta accaagaaac
     4801 tttttcccat agttcctcct gtccttgcta tcaaaatgtc attttcttcc aatcttaaat
     4861 caactagttt agaattacta atatcacagt aaggcacact ggaccaattt acacgtccct
     4921 cttgtatatc tgtgattctc aaaaacttaa cattgccttg ttttttcgcc gaatctgtat
     4981 agccatattg aatcttagta gaaatacttc ctaaataaac ccatagtcga acatcgctag
     5041 atgatttttc ttgtcgttct ttatgaacaa cagtgatcat cttgagttgt ccgactattt
     5101 tttagttgca gcaatctttt tgggacgctt atttgcatga agcaacgttg ctaagtctag
     5161 taataaaaca ttggctaaca caggattggc tttctcgcga tacgctgcgg cgccactttc
     5221 aaacaatact gctagacgat cttctacaac aacgatttgt tccaaatacg gtggaaattt
     5281 gattcgtttt aggatccggc taggagagtc ccgctcggac actaagagtt ccgaggtata
     5341 aggaccataa gaacgactgg ctaagagata atggccataa aaagccacac cttgaatctt
     5401 atcagatgtt tttctgctca ttgtcacggt tggttggatt ttgtctttgc ggcgaattgc
     5461 tgttttcatt tcagctatcg tcggcaatag atagaaagtc aactgtcctt caccttttag
     5521 tgtgaagttg ccagatacca gttgatcatt gttttgagtc aaatacgatg cttgtggaat
     5581 ctctgagaga tcagttgtca gccaataagc gattggctgt cccgtctctt cagatgtatc
     5641 tgctaagata tcgatcaaac gtaaagcggc tact
//
LOCUS       DAIGOK010000134         5466 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.135, whole genome shotgun sequence.
ACCESSION   DAIGOK010000134 DAIGOK010000000
VERSION     DAIGOK010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.135"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            118..1107
                     /locus_tag="NOA01_002337"
     CDS             118..1107
                     /locus_tag="NOA01_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCI0633503.1"
                     /translation="MRDKEMRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAG
                     QYLFDKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGA
                     MTICLSNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWET
                     CLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFME
                     MQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVL
                     DQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1089..1550
                     /locus_tag="NOA01_002338"
     CDS             1089..1550
                     /locus_tag="NOA01_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCI0633504.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLVLMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1600..2064
                     /locus_tag="NOA01_002339"
     CDS             1600..2064
                     /locus_tag="NOA01_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCI0633505.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2078..2827
                     /locus_tag="NOA01_002340"
     CDS             2078..2827
                     /locus_tag="NOA01_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCI0633506.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2827..3657
                     /locus_tag="NOA01_002341"
     CDS             2827..3657
                     /locus_tag="NOA01_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCI0633507.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3657..4304
                     /locus_tag="NOA01_002342"
     CDS             3657..4304
                     /locus_tag="NOA01_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCI0633508.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4402..5178)
                     /locus_tag="NOA01_002343"
     CDS             complement(4402..5178)
                     /locus_tag="NOA01_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCI0633509.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5260..5403
                     /locus_tag="NOA01_002344"
                     /pseudo
     CDS             5260..5403
                     /locus_tag="NOA01_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
BASE COUNT         1871 a          739 c          974 g         1882 t
ORIGIN      
        1 attttattat ataaaattgt tataaaaaat tttttttgaa aaatagtatt gcaaaataaa
       61 gcaatttcat agataatagg tatcgtaaag ttatataact ttataatacc tattagagtg
      121 agggataaag aaatgagaca actacacaaa gaacagattg aaacagcaat ggcagcgtta
      181 aaggaaaaga caattgatca tgtatatttt acagcatgtg ggggttcaca agccgtttta
      241 atggctggtc aatatctatt tgataaagaa ttaactgtac cttgtcatgt ttatactgct
      301 aacgaattta actatgctat accaaaagga ttatctgaaa attctttagt tatcacttgt
      361 tcccattctg gcacaacacc agaaactgta gaagcaacaa aaaaagcaag agaatttgga
      421 gcaatgacta tttgtttatc taatgaagaa ggatctcctc tatgggaagc agctgaatat
      481 cctgttcact atgattgggg taaagatgtt gatgctagtg atttaaataa aggaattctt
      541 tatggtttat tatttaatat tttacttgtt ttagatggct ctaaaaaatg ggaaacttgc
      601 ttgaatgaat ttgataagtt aaatgatctt gttattgctg aaaagagcaa atttgaaata
      661 actgctaaag aatggggtaa aaaccaaaaa cgagataaaa ttatttacac aattggtagc
      721 ggtattaatt acggagaaat ttactccaca gcagcttgct ggttcatgga aatgcaatgg
      781 attaattcta atgctattca ttctggggaa tttttccatg gtccttttga aatcactgat
      841 tatgatgtac cattcatctt agtgaaaagt attggagata caagattttt agatcaacgg
      901 gtagaagact tcactaaaaa attcagtaat aaattaatgg ttttagacca agctgatttt
      961 gacttagaat ctatagatag tgtagcgaaa gaatatattg cagctgtact tagtggcgtt
     1021 gttattcgcc aaatggtaga agcgattgct tttgaaagag gccattcctt agatgttaga
     1081 cgctatatgt ggcaaatgaa atattaatta ataggaggtt gcaaaagatg gcaaatcaat
     1141 tatttcacat tgtgattaca acacattcta ccctttgtga gggttattta aaggcaacaa
     1201 aattagtttt aatgaatgac gccacaggta ttagtgctgt tccatttaaa gaaaatacta
     1261 gtccagatga ttttgaggag agtatgaaag aaataattag tcagcatcat ttgcaacctc
     1321 taattatttt aacggattta attggaggaa cacctaataa tgtttctatt aaacagttga
     1381 acaacaatag aattcaaatt attgctggga ttaatttacc tcttttactg gagttattaa
     1441 tggcccagtc agctggtaaa tctatagaag aaatagattt taaggaatta ctgaataact
     1501 ctcaaaaaag tatggttcat attaattcgc ttttaaaaga tgtcagttaa ggtggcaatt
     1561 tctattctat aaattttaaa aattgaagga ggaaatagaa tgattaaatt agtgcgtgta
     1621 gaccacagat tacttcatgg acaagtaata ttttcttgga ctaaacagta tgatattaac
     1681 catattattg tggctgataa caaagttcca ggagatccaa tgtctgtgat ggctttgaca
     1741 attgctaaac ctgctgattg caaattagac attgttaaaa ttagtgatgt taaagggatt
     1801 atggaaacat ccccaaatga taattacatg attttaatta aaggacctaa ggaagctttt
     1861 gaattagttc aaaatttacc agaaattaaa gaaattaatt ttggcggtgt gtctaagaaa
     1921 agcgagtcca aacaatatgg aaaggcagtc tttttaactc ctgaagaatt agaagccaca
     1981 aaaaaaattc ttgatcaaga agtgaaaata tttgtgcaac aagtcccatc ttcaagtatt
     2041 gaaaatgtag attttagtaa gtaagggggg aaaataaatg ttattaaaag ccattttatt
     2101 aggtttagtt ggtgtggtag ctgttattga ctccaggtta attggtcgac aaaatattgg
     2161 tagaccacta attttgagta cacttgccgg acttgtgcta ggtgacttgc atacagggat
     2221 tgttcttggg gcatctttag aattaatttc tatgggattc gtctcaattg gggcggctgg
     2281 acctcctaat atgcaacttg gtagtattat tgccactgct tttgcaattt taactaatag
     2341 tagtacagaa acagccttaa caatagctat cccagtggct gtagctggtg aatttatgag
     2401 tattatcatg cgtatgataa ttgctcaatt tggacatgct gcagataaag ccattgaaaa
     2461 taataaattt agaaaagctc aaattattca tatttattgg tcatttattt ttaatgcttt
     2521 tgtttacttt attccaatct ttttaacagt atattttggt gctgacgttg tggcagattt
     2581 agtagataaa attccagaaa tgattacaga cgccttgact gtctctggaa atatgctttc
     2641 cgcattaggg tttgcaatgc tattaagcac aatgttgagt aaaaaattat atccttactt
     2701 tatttttgga ttctttattg ttgcctattc aggtctttca ctaattggcg tgacaatttt
     2761 tgcagctctg attgcctttg taatggatca agttaaatat ggaaaaagag aagaggcgca
     2821 tggttaatgg aaaatagtac agaaaaaaaa cgtttaatca caaaaaaaga tcttcataag
     2881 atgtgtcttc gttcatggac aatcatgtgt tcgtggaatt atgaaagaca aatgcatatg
     2941 ggatttatgt atgggatggc gccaatttta aataaagttt ataaaaatga tgaagagaaa
     3001 aaaagagaag cttatactcg tcatatggca ttttttaact gtacgcctca aatgactcca
     3061 tttattatgg gactttgtgc ttctatggaa gaacaaaatg ctcaagcaga agaaggaaac
     3121 tttaatactg aatctatctc catgattaaa accagtttaa tgggtccttt tgctggaatt
     3181 ggtgattcat ttttccaagg tactttaaga attattacat ttgggattgg attgtctttt
     3241 gctaagcaag gtagtatttt aggaccgatt ttagctgttg gcctttttgc tataccttct
     3301 attttagctg ttttttacgg cggtaaatta ggatatgagt ctggcaataa ataccttact
     3361 aaattgtatc aagaaggtct tatggatcga gtaatgcaat ttgcttctat cattggtttg
     3421 gctgtagtag gtggaatggt ggctagcatg gtgccaattg tcaccccttt aaaattcact
     3481 acagggggaa cagaacttgt tttacaagac ttacttgatt ccattattcc tggattatta
     3541 ccacttgtat gtactttggg aatatataaa ttgattaaga aaaatgttag tacaaatatg
     3601 ctcttattag gttgtatttt ggcagggttt gttttaagta taataggtat cttataatga
     3661 ttaaggctct aatttttgat atggacggag ttttaattga tagcgagatt atgtatcaaa
     3721 agtgggtatt taatttttta aaacaagaaa atttaagcta tcctttgtat tcttatcaaa
     3781 aaaagattgg aactacagca agtatttttg atgatataga aaagtacaat gagggatgta
     3841 acagtttaga attgaaggga aaatttacta aatattggaa atcgcaaaag attgattact
     3901 tggatatttt tcctaatata ctaattgaac agcttgaatg gttaaaaaag aaaaaaataa
     3961 aaatggcaat tgcttcttcc agtccaatgg atactataaa tgaagttgtt accacttgta
     4021 aaatagagtc atattttgat tgtttaatta gcggaagaga tttgccagaa agtaaaccta
     4081 atcctactat attccttaaa gcttcagaac agctcagaat tccagttgaa gaatgtctgg
     4141 tgatagaaga ttcttataat ggtataaaag ctggtaaaag agcaaatatg aaggttcttg
     4201 caattaaaga taaacgtttt tctcaagatg taagtctagc agatgatgtt atttacgata
     4261 ttaaatatct gcgacaagaa gtacaggtga aatttggaat ataaataagt tttttgatgt
     4321 atttagagaa tattagtaaa aataaaaatc atgcccaaat ctaatcaaaa ttgattgctt
     4381 tgggtatgat ttcttttaat atcaatctat tactttagga tctgaatcat ttttatgcac
     4441 cgtatctaaa gtgatatttt ctgtgacttc aataacataa ctaattctat cacaacgaac
     4501 tgtggattga gaataatgaa atggttgatt atttttatct ttgccaacct tttctaaaat
     4561 aaatagcgga tctcctacta tacattgcaa taagtcggaa agctcaggtg ttgcagataa
     4621 accgtttatt tccataatag agctcgtagc tttgacatca tagtcatctt ctagaagttt
     4681 atataatgaa gtatattggc tacctttttc aaataaagtt ggaaattttt cttctgatgc
     4741 gaaaaaatta tcaattccca tcggggcacc gtcttcccac attaaacgtt taatattgta
     4801 aactctatca gttacattga tttgtaattt attagctaaa aactcatcag cctttataat
     4861 tttagaatgt aaaacagtat atctaatttt atgtcctcgg gtagaaagag aatcgctaaa
     4921 tccatgaact ccagctaatt ttaaactttc ttttttttga gttacaaagg tccctttccc
     4981 ccttaatcgt tcaactgttc cactttctgt taaaagttta aaagcttttc gaatggtaat
     5041 cttacttact ccataaattt tacataattc ttcttctgta ggtaattgct ctcctggaag
     5101 atattttttt tcacgaatat cgttacgtat agaagcttct acttgtagat ataatggtct
     5161 attatcttga aaattcatag tacactccct aaaaagttat acttcattat atcacaattt
     5221 attaaaataa aagattaaga tataaatata aatgaactag tctttgacta accaatgccc
     5281 gcttttgaaa gaaaacaaat tcattctgtt ttaagtaaaa atccttatgt taccactcat
     5341 tctgaagggg atgagcctta tcgctacttg gttgtggaac caagcgataa gacaatttta
     5401 taaattcaat attaattaaa ataaaaacaa acaaagtaga ttattttact ttgtttgttt
     5461 ttttat
//
LOCUS       DAIGOK010000135         5414 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.136, whole genome shotgun sequence.
ACCESSION   DAIGOK010000135 DAIGOK010000000
VERSION     DAIGOK010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.136"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(152..427)
                     /locus_tag="NOA01_002345"
     CDS             complement(152..427)
                     /locus_tag="NOA01_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HCI0633510.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(399..1352)
                     /locus_tag="NOA01_002346"
     CDS             complement(399..1352)
                     /locus_tag="NOA01_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCI0633511.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            1964..3457
                     /locus_tag="NOA01_002347"
     CDS             1964..3457
                     /locus_tag="NOA01_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HCI0633512.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            3592..3888
                     /locus_tag="NOA01_002348"
     CDS             3592..3888
                     /locus_tag="NOA01_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HCI0633513.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            3989..4840
                     /locus_tag="NOA01_002349"
     CDS             3989..4840
                     /locus_tag="NOA01_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633514.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            4861..5144
                     /locus_tag="NOA01_002350"
                     /pseudo
     CDS             4861..5144
                     /locus_tag="NOA01_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1864 a          834 c         1009 g         1707 t
ORIGIN      
        1 atttagtata gtaaaaacta gaaacaataa aatttgtgaa tgttgtatac ccccaaacaa
       61 tacacatatt ttgaaggatt aggattaata aagctaaaaa tatagtaagt atactaaata
      121 tatttactat atctagtata cttactatat tttaatcttt tgatcgtcta ttatatataa
      181 tccgttcctc gtcgtttaaa gtatcaacat aggcgtccat tgcaagtttg actatttcat
      241 acataggaat atcttttgca taactaagat cccgaatggt ttttagtacg ggaggatcta
      301 cttgaactgg ttttctttct ttcgcagtgt actttcgttc ccctaactcg acattaatat
      361 catctttaat tgaaatatct tcagcaccaa ttttcttctt atctttgttc gttggtaata
      421 atgccattta gtttaatctc ctttccaaga ggtaggatat gattggccat tgagtcagca
      481 tatttaggtg tataatcaaa tccttcaata tcaccatgtt gttcatagta ttcaattctt
      541 tcttctaatt ctgtaaagat gtctgcaaaa atagcccacc atcttcgatc tacataatca
      601 tttaattgga taccattctc accatacatt tctaaccgat ccgagccttt aacaatgctt
      661 ttaaaaatgt ttgattcacc atacttatca attgtttttt gatagtttgc gtgttgagag
      721 gaacgtcgca ctgagaaaag aacaggcaaa attccaatga tttgaagatg agactcttca
      781 aaattcgtga caatcggaac taaaacttta ttaataaaat cttctgttcc ttccattgcg
      841 taccgtttta attcttgcat tgcaataata taatctgcag cagttaagaa tgcacgaacc
      901 acattatctg ttgatggtgg gcagtcgata aagatataat cgaaattttt tctcaattct
      961 tctaaaggtt taatgaaggc caatgatctc ttacgacgat caggatattt ttcggacaag
     1021 tattcattaa gttcttcaga tccaggagag ccagccataa tatataaatg tggacttact
     1081 tgagtaatac ctgaaaccca gtcccctttt ttgattgctg cagacatgga tcgatcaaag
     1141 ctagtaattt gatacgttga tccaatgttg gaagtagtat ttctttgttg gtcataatca
     1201 attagcaaca cgtctttttc aaaaagttga ctagaaacaa tcgctaataa atcattgtct
     1261 gtagtcttac caatgccgcc ttttctacct gtaagcatta caacttttcc tagtttatct
     1321 gatgttttaa atgcagtccg taagttttcc atagttccct ccaatttcct atactaaata
     1381 tatttagtat acttcatata cttaatatat tcgatatatc taatatactc caaattgata
     1441 agaattcaga ctttataagg aatatagtgg atatactaaa tatatttgct atattcagta
     1501 tatcagatat attcaaaatg tcaaaatata tttgtgcgtt ttgcgttgac aatttagagt
     1561 gaaaagaggt aaacttaatt ctatagaaac cacagataat tcactattta caaagtagca
     1621 aaagcagtgg tactctttaa acagaaaaat tacataaaaa aaagagacgg aaataattga
     1681 ttttagcaga atcaattatt tctgaatcac atggttagga catgtgttca cgctcgtctc
     1741 aagaatatgt aagtagctac caactactta tattaaaagt tgatcggata ccttttccga
     1801 ttgcctttat tctaccaaaa aaaggcgagc aactcaacag aagaaagttt atttagtgta
     1861 cttaaattaa atattcttaa acactgaaga gagaacacat actatggctg agattaaagc
     1921 taaactgtgt tctctttttg tttgagaaag gacgttacaa tcgatgttga gcgaaattta
     1981 ttcagctatt cttaaaaacg gcttacgtac atacaaatat cgaaacagca ccatgaaacc
     2041 tgcaggtcat attgattcca gtaggagtgg tgctatcttt gcgtaccgta gcaagcaatt
     2101 aatgaatgtt gggcgtggta tggtcattac ttcggaagaa gctattttag agaacgaaaa
     2161 gaaactaacg cattggacac ctaataccta tcgctttggt acttatgcgg atgattatcg
     2221 aacagtggtt aaaggtcatt ctgagaaaaa cttaagtcaa atcaacacat ttgtagttga
     2281 tatcgattca aaagagaacc atcaaggcga aattatttta gcctgtttag atcaagtggg
     2341 ctacatgcca acgctaattt tagaatcaga tcatggttat caagtttatt ttgtactaaa
     2401 gacaccaggc tatgtcacaa agaaaagcaa tttcaaggtg atcgatgtag caaaaaaaat
     2461 ttcaaaaaca attcgtctac agttggctga aaaattgtcc ggagtggatc taggatgtaa
     2521 ccacttcgga attgctcgat tcccacaaga aaataatatt gtttttaaag aaatgaacta
     2581 tcagtattct tttgctgact ggattcaatg gtcgatgaag cttgaatcag atcaaaaaac
     2641 aaaagaagat cgagaacgaa aactaattgt ctttcctgag aaaaaagaaa ttcgtcaagt
     2701 agatgaaccg tggttcgata tgttacttca taaagcaaac atcgtcggcg gagaaggacg
     2761 tcttggtcga aataatgtga ttttcacgct ttctttagct tattactctt caggttatgc
     2821 attggatact tgcgaataca atatgttcac attcaacgaa cggttacagg aaccgctatc
     2881 tgaagcagaa ttagtaaaga ttgtacgtag tgcttattct ggtaattatc aagcagcaag
     2941 cagagaattc attattgaat tgtgccagca atgggtttca ccaaccttaa aaaataatga
     3001 tctgttcatt caacgacgtg gctggtggaa atttaaaaag ccacgagatc aaagaaaata
     3061 tagtcataaa aatgaatgga aagaagatct attggcttat ctaagcgaaa aaagtcttgc
     3121 tagtgagcct tatttgaacc tgtcaaaaaa agaattggct tcacaactaa cgatgcccaa
     3181 gcgaacactt gataagatct taaatgagtt acgagaggag aataaagtct tctacaaggt
     3241 aacacgagga agaaatggcg gaatgatcat tgcttcagtc aaaacgttgt tcgcgagggt
     3301 tattcaacta agtaaagaaa agagactaga ttattcaaca aggatcacag aggtatttgg
     3361 actcaacgag agcttagtag aagagacatt tagacaatta tcaaccccct tgaaccctct
     3421 taaagaagtg aatctttttg aagtagatac aggttgaaaa ataaaaggtg caaattgccc
     3481 tatacaatat atctatgata cttctgtagg tgttgctccc tttgcctagc tacgtgtagg
     3541 tggtatactt ataactgcgt atgcagtctt atgtatcatg gaggaagttg tatgtcgtta
     3601 aaaaattatg tctattcaca tcctgttaat gtgtttatca ttggtcgttt gggattatct
     3661 gtggaagtgt tctgtgagct ttatggtttt aagcagggga ctttgtcttc ttgggttacc
     3721 agggaaaaaa cagttgcttc tttgccgatt gagtttcttc atgggttgtc cttagcttct
     3781 ggggagacta tggatcaggt atatgattgt ttgtgtgtgt tagagcagga gtatattgag
     3841 tataaaatag ctaacgagtt aagaaaaagg aagaagtata tccagtagta gtttttattt
     3901 acttaaaaaa ttggttcatt gagcaaccta tcgctgctaa agtagcaaag gaaaacgtta
     3961 aaggaaagaa tttagggaag aaatcgaaat gaaatcggac gactggattg aacacacctg
     4021 gcttcctctg aaaagaaata gcaaacgaaa gaaattttgg gtattgcaat tatggccatt
     4081 aggaaaactt tgtagttacg gggcacgact tattcttgat ttaaccgttt actcgattgg
     4141 agtatttgga acggcttatt acttgataaa tgatcccaat aggattgcag catcagacat
     4201 ttttacagtg ctaatgacgt tgatttttgt catgctattg tctattttta ctcaattagt
     4261 catgcaattt accaggcaaa aattaacagc aggaaaactt ttcaaaagcc tattcgattt
     4321 aattgttgta aatgcaattc tagcgacttt aataaaacaa attgaacaat ctggaagaac
     4381 aacaaaagga gtgaaggatt ttttattgac gtttattcac tttgtaaatg gacacatgta
     4441 tttgtttgaa gtgggtatta ttctcttttt tgtcgggtat gtatccacga aacttattaa
     4501 acgttcaatt gaaaaagaaa ttgaaattga cctgattccg acacttcttg gttatgagta
     4561 caatgttgat tttggaagat atgacgaatt gatcaatacg gatatcgttc acacgagtat
     4621 ccatgaatct actgttaagg gcatcagatc tattttaaaa aatggccagg aaatgaaagc
     4681 aatagatcga agaaaagaga tctacaattt ttatgaagtc gaagcaagcc tacgatatgg
     4741 catcggaaaa ttgtggttag atggaagtaa tattcgtcaa aacaaaccat acattttttg
     4801 gaacagtagt gatttagaac ttccaatgaa agataaataa ttatggagaa tcgtttgaaa
     4861 atgattcctc tatacaggat tgtgcttata gttggcatgg agttaaaaac cttaaagaag
     4921 gagcagttat tcaaagtgaa agaacgtcat aatcagttac tatgtagtaa aacgggtacg
     4981 atgaacattg aataccgaac agaatattta gaattaacga atcaaactga ttttttgttg
     5041 attcgaaagc aacaagagca agaaaaaaat taatggctac ttatagccaa ttgattgatg
     5101 aatgggaaca attcaaggaa tctaaaattc cagaacgtct ctaaaagcct ttatttgtaa
     5161 agagcataag gaaacgtggg aacacttttt aaaaaaagtg ttccaatagg ctgtgcctat
     5221 tacgtttctc aaaaataaat gtgggaacaa aatatttaaa aataggatag tgaaaggata
     5281 gtgaaggata gtaaaaggag ggtagtagga tagcaaaaag gataggctcc ttttcgctat
     5341 cctttttaaa ttggaggaat taatatggaa gtaagaataa gaaatgtgga tgagaaaatc
     5401 gtaagagaaa ttga
//
LOCUS       DAIGOK010000136         5381 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.137, whole genome shotgun sequence.
ACCESSION   DAIGOK010000136 DAIGOK010000000
VERSION     DAIGOK010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.137"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(142..1290)
                     /gene="nagA"
                     /locus_tag="NOA01_002351"
     CDS             complement(142..1290)
                     /gene="nagA"
                     /locus_tag="NOA01_002351"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCI0633515.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1366..2202)
                     /locus_tag="NOA01_002352"
     CDS             complement(1366..2202)
                     /locus_tag="NOA01_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCI0633516.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2386..3603)
                     /locus_tag="NOA01_002353"
     CDS             complement(2386..3603)
                     /locus_tag="NOA01_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCI0633517.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3752..3931)
                     /locus_tag="NOA01_002354"
     CDS             complement(3752..3931)
                     /locus_tag="NOA01_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633518.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3986..4069
                     /locus_tag="NOA01_002355"
     tRNA            3986..4069
                     /locus_tag="NOA01_002355"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4020..4022,aa:Leu,seq:gag)
     gene            complement(4309..5307)
                     /locus_tag="NOA01_002356"
     CDS             complement(4309..5307)
                     /locus_tag="NOA01_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633519.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLIFLLTYRKC
                     QNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLS
                     LFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVW
                     ASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         1681 a         1088 c          847 g         1765 t
ORIGIN      
        1 ttatctttac attgttactt attgaacaat aggaaattaa agaaactttt gaaacttatg
       61 taaagtgatt tttactagaa cacaaaaaaa accttaaaac caaacttaag aagtttagtt
      121 ttaaggttaa cttaaattat attatgcgtg gaaacgttcc acgccatcta gataagttgc
      181 ttccaatgtc atatcagggt tcaatacgat aaagtctgca tcgcgtcctt gagcgatttt
      241 tccacatgta tcatcgattt tacagctgat tgctggaatc agactagcca tcatgactgc
      301 ttgttctggt gttgctaatc cccagtctac aacattttta attgcttctt tcaatttcaa
      361 tatacttcca gctaagttgc cagattctag acgtgctgtt ccttctttta ctactactgg
      421 aaattcgcct aaattgtaat ttccatcagg catcccaccg gccatcatac agtctgtaat
      481 taatgcgaca tgatcatgtc ctgctttttc cattaggatt cccgcagcat ttggatgtac
      541 atgatggcca tcacaaatca attcagaaaa gacatgttct aaagataata acgcacccac
      601 catacctggt tcgcgatgat tcaatccacg cataccattg aaagcatgga caaatacact
      661 tgcgcctgct tcaacagctt tttgcgcttc ttctagtgta gcattactgt gtcctagaga
      721 aacaactact ccttcttcag tcacttgttt gacaaactct tctacacctt gacgttccgg
      781 tgctaaagca atttttttga ttaaaccacc tgaagcttct tgccattcat ggaaagtatc
      841 caaatcagga tccccgaaat aacttgggtt ttgtgcccct ttgtgttctt ctgtgaagaa
      901 aggaccttca aaatagattc ctttgatttt agctcctggt gcttcctgat acattttgcc
      961 gattgtctca gctacatctt ttaaacgttc tttgcttgac gtcaatgttg ttggtaaaaa
     1021 tgaagtcact ccacaagata acagaccttc agacatcact ttgatgcctt ctgcatcatt
     1081 atccattaca tcatgattca tatagccgtg aatatgtgta tctaccaaac caggagcaat
     1141 ccatttgccg ctttggtcaa tgatgttgac ttctccctca ggtagttctt tggtgtattc
     1201 accaaaaagt ccatcggtaa tttcaagata gcctgcatgt ttgacacctg acgctaaaaa
     1261 gaatttgtca gcatagataa aagttctcat tctattctct ccctttgttt ttctacctct
     1321 aaagtactct tatttagaga agaagtcaag aatggtctac accatttaca aattttttga
     1381 aatttgtttc acagcttgtg ctaagccttc atagaatgct gctctttctg gttcttcttt
     1441 acgcttgtat attaagcttt taagcacaaa taaatcattg aggtaatagt agctattctt
     1501 ctcttgagcc caatagattc cttgattcac ctgttctaac gcttcgtcga tttcattttg
     1561 atggaataag aaatttccat aattataaaa aatttgcgaa aattcacttt ttgatcgttc
     1621 atcagctgat tgatgcagca gttggatgct tcgactcaaa tagtagcgtg cttcagccgt
     1681 ttttcctgcc acaccatata tttttccgat acaactaatc agttgtattt ctgtgtcaga
     1741 aacatggatt ctttcttttt ggctagagta gcttaatgct tctttcaaat aataaaggga
     1801 ctgagagata ttttgacgca acgtgaattc gcaaacaccg agataataat aatagcgttg
     1861 aaagtcagtt gcttgataca gattttcaga aatatcagaa gattttagta cttgttctaa
     1921 cttccgataa tcacgttttt ggaaacaact atccaacagt tcaaataatt tatcgtttgt
     1981 ccgagtagtt tttcgagaga gattcatcac atgatcaatc gttaccccta gccgttgaca
     2041 aatctgcatc atcaccaata cattcggcag ttcctcatta ttttcgatcc gacttaatac
     2101 actttgtgaa caaatatcat gcgaaagcat tttttgcgtc attttttgtt cttttcgaat
     2161 ctctttaatg atcgttccaa aggactcgat taacgtttcc ataagtatcc tccccaaaat
     2221 atgcgtattc gcattctatt cgtattttac tttaggaagt cgcaaacttc aagaaaagac
     2281 ttgcaactaa ataatatact ttagttattt aagaaaactc cactagaaag agagcctcag
     2341 tgtgtttgtc tgtggccctc ctactgaaaa atttggtttt taaaatcatt tttggcgata
     2401 ggttgctagg aattctctca ttttcttagc tgtttgtttc aaatcatcca ttttaggaag
     2461 ataaacgatt cgaaaatgat ctggctggtt ccaattaaat ccaccaccat gtacaagtag
     2521 aatatgatgt tcatgtaaaa agtccaaaac gaatttttca tcatccaaaa tattgaatcg
     2581 ctttgtatca atttttggga atatataaaa agcagctttt ggttttacgg ctgaaagacc
     2641 aggaatatca ttgattgcat tatagatata ttctctttgt tcatagatcc ttccgcctgg
     2701 caggagcagc tcatcgacac tttgataacc gcctagcgcg gtttggataa tttgctgaga
     2761 caatacattg gaacacaaac gcatggagga caacatattc agcccttcta tatagccttt
     2821 gacgtgtgac ttatctccac ttaggaccat ccagcctact cgaaatcctg ctacacgatg
     2881 agatttggat agaccgttca gcgtaacaac gaacaaatca ggcgctaatg tagcaatcgg
     2941 aatatgttct agaccatcca tcactaagcg atcatagatt tcgtcggaat aaataatcag
     3001 gttattttgt cgagctactt caacgatttc ttctaataat tcttttggat aaagtgcccc
     3061 agtaggatta ttaggattaa tgataacaat agcttttgtc cgactcgtta tttttgattt
     3121 gatatcatca atatcaggat accattctgc ctcttcatcg caaatataat gaacaggtgt
     3181 gcctccagct aatgcaatag aagccgtcca caacggatag tccggcattg gaaccagtac
     3241 ttcatctcca ttgtccagca acccctgcat acacatcgtg atcaattcgc tcactccgtt
     3301 gccggtgtag atatcattga tcgtcacatt cggaaaatgt ttcagttggc aatattgctc
     3361 aattgctttt ctagcagaaa agatcccctt ggaatctgag tagccttctg agtcacgggc
     3421 attcatgatc aaatcacgaa tgacttcgtt tggtgcttca aatccaaaag gtgcgggatt
     3481 tcccgtgttc aattttaaaa tactaatgcc ctcttcttgc atccgctccg cttcttctag
     3541 aacgggaccg cgaacatcgt aactgacacc ttctaatttg ttggattttt caaattctct
     3601 catcacttag ccaatccctc atttcaaaaa gttagaactt tccaaatttt ttgttattta
     3661 ctatttcaat atcatactcc atcctagaaa attttgccac ttatttagga aaaacgtaag
     3721 tattaaaaaa acacaaacaa gcgttgagaa cttaattgtc atgatagtca ttccatagat
     3781 tcgctgccca atccattgca attccacaaa tgattcctag cataaaacca agcaaaaaac
     3841 gtccgaatgc aagtccaatc aatacaccta agatcattcc cagtcctgaa aattctccgt
     3901 catatttctc catcttaata agtcctccca tttttgagct tgacaactat gtaaaacctc
     3961 gctatcatta gtgtgttatt attacgcaga tgtgttggaa ttggcagaca agcatgattg
     4021 agggtcatgt gagcgttagc tcgtgtgggt tcgagtccca tcatctgcac tacaactcgt
     4081 gaagttaatt ttgcttcacg agtttttttg tttgaacgtt tctttatgcc attatataca
     4141 tatcactgta tcctttacaa aaagaacgta caatcatgta aacagcctga ggtgagtata
     4201 tgaaggtcgg aaacaagcta ctcgtagaaa cttgcaagtg ttaaaacaat taatcgaaaa
     4261 acgagtaaaa tccctcacct aatactaaag cgagggattt tttgtatatt atctgtttat
     4321 caccgttatt ataaaggtac aaataccaag cacggttaat ccaaattgca taaattcatt
     4381 tttttgtaca ctcatgcttt tcattagctc tttaactgcc attagtacta agatggcgat
     4441 aacaacataa agtatatagt acgtccgcat tccggaagcc catactgtgg gagaaaatcc
     4501 cataattaac cttgaacaaa atcccattat catcaaaata actaaaaaaa tagaagtttt
     4561 cttattgtca aaacttaaat aaattccaac aattaaacat agtaataatg ccaagactac
     4621 taaagtagct atccaagtac ctggataaaa aagtgacagt ttcgttccta actctgtaaa
     4681 gagattattc aggttactac tactccaaat cattgccctt ttatttccat tgacgtaagt
     4741 aaagctttga cccattgtat taccaaaata aataatcagg tttaagaaaa acggtaatgc
     4801 agtcaatata cgcacataat agttttgaca tttcctatat gttagaagga aaattaagaa
     4861 aaatagcaat aaaaataaga tattcatgtc taaaaaaaac ggctttccaa atgatgaaat
     4921 acctaaatct aacttattaa gaaagttgaa atttttatat tcaggaaacc atttattaat
     4981 ttcatgtgca gcacgtaatg cattccctgg agttgtcaaa gagaaaatta attctgctaa
     5041 gcttattatt gaaaatactg ataacttata attatatcgt ccattgacaa tcaaatataa
     5101 actaactata cttgttagaa caaaaaaaca ggcgttgact tgttcttgat tagttaaaaa
     5161 tatcaataaa ggaatactta cccaatatat ttttctattt atttcttctc ctcttaagag
     5221 ttgaaaaaac ggataaaaag ttagtaaact aaatgctact ggccactgat agttcaaggt
     5281 ggttgctctc caaccggttt cccccattac tgtcaatggt attaaacagt atatagagca
     5341 aaccaataat aaatttttaa catttatttt ctcaaaaaca t
//
LOCUS       DAIGOK010000137         5280 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.138, whole genome shotgun sequence.
ACCESSION   DAIGOK010000137 DAIGOK010000000
VERSION     DAIGOK010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5280
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.138"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(<1..229)
                     /locus_tag="NOA01_002357"
     CDS             complement(<1..229)
                     /locus_tag="NOA01_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633520.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEF"
     gene            complement(527..1501)
                     /gene="sgrA"
                     /locus_tag="NOA01_002358"
     CDS             complement(527..1501)
                     /gene="sgrA"
                     /locus_tag="NOA01_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HCI0633521.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(1697..2536)
                     /locus_tag="NOA01_002359"
     CDS             complement(1697..2536)
                     /locus_tag="NOA01_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCI0633522.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            2721..3608
                     /locus_tag="NOA01_002360"
     CDS             2721..3608
                     /locus_tag="NOA01_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCI0633523.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(4202..4897)
                     /locus_tag="NOA01_002361"
     CDS             complement(4202..4897)
                     /locus_tag="NOA01_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCI0633524.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(4881..5279)
                     /locus_tag="NOA01_002362"
     CDS             complement(4881..5279)
                     /locus_tag="NOA01_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCI0633525.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         1677 a         1067 c          775 g         1761 t
ORIGIN      
        1 aaaactcgac ttttatcatc ttgttttctg aaagaaaaga taatttatat tctggatacc
       61 tttcgcaaaa cgattctgct tgttgaatca ccgaattcat cttagccagg gtgtagttta
      121 gatctaaagc gatatccttc accacatata atttctcgtc tctttcataa caatagttca
      181 aaaattgata tacttgcctt tcattgatat caataaattc ttcaaacaaa gtacatactc
      241 ctttttatct attatttaga cttattccga cagctacatg aataacgtac aaatagattc
      301 gttactgatt tttcagagat actaccatgc gcagtgtgca ttgtcaatta ttcttgctct
      361 tattttccat ttgtgttcct gttatttcct gtttctcaaa tatgttagta aacaaaaata
      421 gatctggcaa agctacaact tcgccaggca atacagcact ctcgaatgaa atattcactc
      481 aaaaatctat cgttttattc aattttagat ctgttgcttt tattctttat ttcactcgat
      541 ggaagagaga acgaaaatag cttgctccta caatcaaaat gagtaacaca cccatccatg
      601 taattagctt gttcaaggtt ctactaccag tttctggtaa atcgcttttt ctattatctg
      661 tttctttcgc tttttcttga tgatcaattc cacgagaaac aaaatcaggc tgttttagca
      721 cactttttag gttgctccct gcatttctct tttgttcctt agttggtatg ataggtacca
      781 aatcaagttc attatttact tttcctgtgc tgctttcgct gctcgtgctt cctgtgctac
      841 tctcactact catgcttcct gtgctacttt cactactcgt actctccgta ctgctttcgc
      901 tgctcgtgct tcctgtgcta ctttcactac tcgtactctc cgtactgctt tcgctgctcg
      961 tgttttctgt gctactttca ctgctcgtac tctctgtact gctttcaccg ctcgtgtttt
     1021 ctgtactgct ttcactactt gtgctatccg tactgcttgg cggtccaaca actgtccaac
     1081 tgcttggttt caaattctcc gtgatcgtca tctgtccatc ataaaaaaca atcaaatcaa
     1141 ctttatcttt actcttgatc tcatcaatcg atagttctgc agaaatcatt attgtatact
     1201 caataccagc ggtccattgt acgtctttta aataaacttc cccaataact ttttctgaac
     1261 ttccattacc atcccaacca tatgttagaa cagcatgtac atcactagca ttcacgcttt
     1321 tgtttggttt gactggaata gtaatgactt gttgtgtcat gtcataaaca atccgatcag
     1381 ttttcacagt aaatgaaaga ttctcatttg cccgttcatt tgcagaagta ctgattccaa
     1441 tcattgctgc aattatactc agcccaagaa taacaaataa tacggtcgct gttttcttca
     1501 tttctatcac tctccatata attaccttct atttgataaa ggaattatat aaaatgaaaa
     1561 tgatgagaac acgacagtca gttgtgaaaa tctaacaaaa gatgtcgtgt attcgcttga
     1621 atttgtttac tttttcgttt aatcaatgac agacttaaaa aagcaagacc gatatcagcc
     1681 ttgctttctt catcttttat tgataaacag cagtgtattt cttcatccgc tttgttttat
     1741 atgcatagag taacaaacca acaatcaaga ctaacaagaa taatgtagtt gatatagatg
     1801 cttcagcccc aaagtttccc ccagtaatta ttgtattttg cgattggatc agagtggaga
     1861 aaaaaccttg ttcttgcgtt ccgctgactg gtatacccaa aatagctcct tgtgtccaat
     1921 tccaagtaat atgagcacca atagccgtaa ataaacttcc tgtactgtag ataagtgcgc
     1981 tgaaaaagca acctgctaaa aagatattga aaactgccag aaatgtcatg ttaggattca
     2041 atccgtgcaa aagagcaaat aatgcagcag atccaatcat tgctattaac ggaccaaatc
     2101 taagcatctg atgctgaaaa aagccacgga ataaaagctc ttctgatgcc cccactgcaa
     2161 taaataaaat aaaactcgtt atcagcggca caatgtttgc gtttcctgaa tctaaccgat
     2221 acgcccccgt caaatataat ggactagtaa taagcagaaa cactccaatt ccaacaacga
     2281 gacctcccgc taaatacccc cagtcttttc ctcgaacaga aacaccataa tcttctattc
     2341 tttttccttc gaccttacta gcaagaaata aagctagtaa aaaaacagct aaatgtccga
     2401 ccgtaccggc aattgctcca atccattcac tatttccaat aaacatatcc gcaagtgcat
     2461 tattgacaag aattcctaaa acaaaaccaa ctaagactat taaaacagat ccaatcaacc
     2521 gaataatcct gatcatcttt catccctcct ttatattaag tttaacaacc cttttctttc
     2581 ttgtctcgtg aaaactatgt aaatgttctc catcatagag agaatagatt tttacgaact
     2641 aagaatatct acagcaacct tacgaaaatg aaagactgtc tccctaattc ttctgttaga
     2701 ctgtttttaa taaggaaagg atgggaaatt ttttgaaaaa acaactactt attatgtgcc
     2761 tttcagcatg tgcaatttta ctattctcag gttgtagcaa gtccaatgaa acgattgcaa
     2821 cgtttaaagg tggaacactt acagttcagg atttttatga taacccaaaa gttaaatcaa
     2881 ataatcaaac actattaaaa aaaatgattg tcttcaaagc atttgatgat gtatacggaa
     2941 aagagatttc ttcaaaggaa gtaacaaaag aatataatga acaaatcaaa aaactcggtc
     3001 caaactacaa ggaacaatta aaagcagttg gccaaacaga agagacatat aagaaactct
     3061 ttaaacagat gctggctttc caatacggtt tgaaagccaa tgtaaaatta acagataaag
     3121 atctagacac tgcttggaaa gaattctatc cagaagttag tacacagatc attctatttt
     3181 cgactaaaga agaagcagat aaagcaaaaa aagaagcaaa tgaaggagaa aatttctcca
     3241 agcttgttca agcttatgga aaaaataaaa cattaaagga aactgatgga aaaatgaatt
     3301 ttgattcaac caatccagag attcctactg aagtaaaaaa agcagcattt aaactaaaaa
     3361 acggggaagt ttctgatatc attcctgtaa cagatccaac gacttatcag caaagctatt
     3421 atctagtaaa aatggtaaaa aaacaggata aaggttctaa taaagacaaa tacaaatctg
     3481 agctagaaaa aattgctacc gaagcaaaaa ctcttgatac ggaattcatg gataaaacaa
     3541 tccgtaaagt gatgaaaaaa gacaatgtaa ctattaaaga tccttatgta aaaaatatat
     3601 tcaaataaac aaatttgacc caattgggga tgtggcaaaa tttgagaagt gatttttgga
     3661 aattccttct tatttttgag gcaaaccact tttgccacaa cttcatcggg gccttttttt
     3721 atttgtacca aaacaaatca ctggatttgt tacaatcatg aggatataat ttaaaaaagt
     3781 tttttatttc tatcacatga atcaatgcgc cattaagtag attctttcat gcttccctat
     3841 tttttcactt gccgtatcgc ttcaaaaaca actatcataa actcaaactc gactgaaaag
     3901 ttattttaaa aaagactaaa aatatccctt ctggacttgt tcttcttatt ctgttatact
     3961 agagaaacac gaaagatatt tttatgcgac acttttcatt tgtttttatg aaatttattc
     4021 aaaactggcg taacttcaga gttacaagga tgtattagag gttagggaat acatcgttta
     4081 aatgagaaat aagtaatagt tagtaatagt cacccaaact ttaaattaat gaatattgaa
     4141 tcgtcagata gcctcctcga gttttcgaga aggctatcgt ttattttatg gagaaattga
     4201 ttcagaagta atcagcgacc ttttttagtt caccaatcgt ttctgcttgg aaagtttgca
     4261 cccaatcttc ttggaagccc ctcactgctt tatcaatcga gggcatctct aatacttgat
     4321 ctagtagctc tttacctaac gttatcgcat gagctccaga tcgaatggct gtagtgactt
     4381 gttcacaatt tttgaaacta gctgctaata tttttgttgg tctttgcgta cgttcgatct
     4441 cagtccgcaa cgaatcaatc actttcatag catcaatgcc ttgattttgc atcctgttat
     4501 aataaggtgc aatataatct gctccattat taatagctaa atacccttgt aattctgtgt
     4561 aaatagctgt accggtaatc aaataacctt cttttttcaa ctttttgatc gcttgaaggc
     4621 ctgcttgatt caccggcacc ttgataaacg tatcttttcc cagttcttct atgataactc
     4681 gagcatcctc tatcatttct tcttccgttc ttcctattgt ctgaatatgg ataggaatct
     4741 gtccgattat ttttttgatt tctttcatat gcgagaagaa gtttgacgct ccttcttttt
     4801 tcagaattgt aggatttgtt gttacgcctg ttaaattcaa aaactgttga caagaataaa
     4861 tactttttgt atttgctgta tcaagcatga tttccatata gctttccccc attctgatcc
     4921 tcttgatcga ttttaatgat tgtccccttt cttaaatgcg gcatttcctt atcttctaca
     4981 tacattgttc ctggaagttc cggctcatga gatccatcaa attttatcgt gatatgtcct
     5041 aattcattca aattagtttg gacaacatct cctactgctg tgattacata catttgttta
     5101 tcaaaacaga gcttcatatc acaagttatt tcttttctta cctgattcaa ttcaatctta
     5161 taacaataat ctgttaatgt ttccggtgct tcatctccaa ataaaatcat tattttttct
     5221 ttcctgaata cttctgcttc tataccaaca tctgttacaa ccgtttcaaa gatcgtcatc
//
LOCUS       DAIGOK010000138         5201 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.139, whole genome shotgun sequence.
ACCESSION   DAIGOK010000138 DAIGOK010000000
VERSION     DAIGOK010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.139"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            25..696
                     /locus_tag="NOA01_002363"
     CDS             25..696
                     /locus_tag="NOA01_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCI0633526.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            755..1195
                     /locus_tag="NOA01_002364"
     CDS             755..1195
                     /locus_tag="NOA01_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633527.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1207..1875
                     /locus_tag="NOA01_002365"
     CDS             1207..1875
                     /locus_tag="NOA01_002365"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCI0633528.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1939..2562
                     /locus_tag="NOA01_002366"
     CDS             1939..2562
                     /locus_tag="NOA01_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HCI0633529.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2618..3004
                     /locus_tag="NOA01_002367"
     CDS             2618..3004
                     /locus_tag="NOA01_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HCI0633530.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3217..4608
                     /locus_tag="NOA01_002368"
     CDS             3217..4608
                     /locus_tag="NOA01_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HCI0633531.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1729 a          861 c         1095 g         1516 t
ORIGIN      
        1 aatgatgaaa aaagggggag aaaaatgaat tttttagttg ttttaaaaga tactctgatt
       61 gagcgatcta catttctata tgttcaattg caggaaaata aaacattgct gcatttttca
      121 ccagagttcc atctagaggg gctaacatta ggagaaatct atcaagcaga agggctgtcg
      181 gctgttttgc acttttttaa ggtagaattg gaattaccag taaaagatta tatcgagctt
      241 tctcttgaat cttgggatcg agcagtggtt gaactgtttc cagaaggttt aatgatcgat
      301 acaaacgatg ggaagatcca tctgactgca gcagaactgc aaaaacaaat gcgttatcaa
      361 atagatgacg aaaattcttt ttctattttt agacgtcagc aaaagattct aaaaagcttt
      421 ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga cacagctgtt gaaaaaatat
      481 ccaggtctgc ttcacacgtc tattcaattt cctcaattga ttacgatgat ccatcgtttt
      541 atacgtatgc aacagctacc atctaagaag ctgtttcttc cattgacgga cacatttcga
      601 gtagtcaaca gagaagataa aaagaaagtg attgtgattg actttaccag aaatcggaat
      661 gtccttcacc aagttgcaat ggaaaaggcc aattaaggct ttttcttttt ggattttttt
      721 gtcataataa atattagatg atggaggaga taatatgatg caaacatggt tagaaaggct
      781 aatacgattt cagcagaact atcacaaact tatgcaggga agatatgcga aatttgatca
      841 gttgaatcgc ggtctattga tcgctgcgct tgtctttagt ttgctaagtc gttggttgcc
      901 ctatagaatc ggacaattgt tgttcatcgt ctttttcgga tggatgatat ttcgtttcct
      961 atcaaaaaaa atctacccca gactaaatga gaatcaacgt tatttgaaat atcttgatag
     1021 agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa gtcaaaatgg atcgaaaaag
     1081 ttatactttt tttgagtgcc cgaattgtca tcaaaagcag cgtgcaccta aaggaaaagg
     1141 acgaatacgt gtgacatgca aaacatgtgg tataaaattc gaaacgaatg tttaagggag
     1201 aaaagaatga aattagtatt attacgtcat ggcgaaagcg aagcaaattt tgaaaattac
     1261 tggacaggat ggctagatgt cgccttaaca gaaaaaggaa aagaacaagc agcagttgcc
     1321 ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg tttatacctc tgttttgaaa
     1381 cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg gtcagttatg gcttcctata
     1441 ataaaaacat ggcgtctaaa tgaacggcat tatggggatc ttgtgggtaa aaacaaagat
     1501 aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat ggcgcagagg atatacggaa
     1561 gttccgccgt taaccgcaga aaatagattt gaccgcagat atgatcactt ggatccgcat
     1621 ttgatcccat acggagaaag tctggaaatg acagtgaatc ggatcattcc cttatggcaa
     1681 gatcatctag caccaaaact aaaagatcgg caagatttac tagttgtggg tcatggtaat
     1741 agcatccgag cactgacaaa atatttagaa gatgttcctg aagatcaaat ggatacgatc
     1801 gatatcccaa atgcacaacc aatccagtat acattagcag atgatttgac aattctggac
     1861 aaaaaaatac tttaaagaac acctttatct aaaagtgaga gatagaaaaa agaagcaaag
     1921 gaatggaaca aaaggaacat gaaaaattat atatgggact ttgatggtac attgtttgat
     1981 acgtatcccg caatggtaga tggtgcatgg caagcactaa aagattttgg tatcagtatg
     2041 gacaaaaaag aaatctactt caaaatgaaa aagtattcta ctagttattt gatcaatgaa
     2101 tcaaacttga atgccagaga gtttaatgaa ttatttcacc gatatgaaaa agaaagtacg
     2161 gaagtttccc gtccatttcc agaaacgaaa caagtactgg aaatgctaaa agacaacggc
     2221 ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa catggggcct gttaaaagaa
     2281 catcgattgg cacatctgat tgaggaagta gtagggatcg atcaggactt tcctcgtaaa
     2341 cctgatccag catctttaaa ttatttgatc gatacttttc atttagaaag aacagacacg
     2401 atgatgatcg gcgatcgtcg tttagatatc gaagcaggga aaaatgcagg agtcgctact
     2461 tgtctttacg acatcgatca ttttctgggt gagatcccgg cagattatgt ggtaggaaat
     2521 ctgaatgaaa tccttacctt gcagagcatc attaaaaagt agtatcccct ttcaaaggag
     2581 atatgttata attttcataa aggagtgata aaattatatg gaaatcagaa gacaaaaaga
     2641 agttgtagaa gcatttcatt atgatatgag attgccgaac caagaagtag aaacagattt
     2701 gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac tatccgaaag aacatagcat
     2761 cgtaggagca cgcttagagt tccgtttagt gtttgataca tatgttttat ctggaagtgt
     2821 gagtcagatc aatcatatcc tcaatcggaa aattgaaggt caaaatgatg tcacacaaga
     2881 agaagtggac gagctagtag caccgttatt cgatatcgta cgccgaatgt cttatgaggt
     2941 gacagagatc gccttagatc agcctggggt aaaattgaat tttcaatcag aaaaacaaga
     3001 atagacagta aaaacatacc tgcgatagat taaagctatc acaggtatgt ttttttatta
     3061 ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg tctaaaatat aaaagtcact
     3121 gaaaaaaatt ttttcaggag gatggttgaa gaatcattct cgggaacctt tacttccttc
     3181 gatggacttg tcattaataa tatgaggagg agaaaaatga caacactcat cacagtttta
     3241 gtggttctag tttttgcggc attattatat gtgttctatc gtatgcaaaa gcgccataca
     3301 aaattttcaa cacgcgtctt tacagcatta ggagttggta tcgtctttgg cggtctgctt
     3361 caattgatct ttggtgtaga cgacaaagtc gttactcaat caatggaatg gatcgggatt
     3421 gtaggtcaag gatatgtatc attcttgcaa atgctagtta tgcctttagt gtttatttcg
     3481 atcattggtg cattcacaaa aatgaaagtt tctgaaaagc tagggaaaat tagtgcgact
     3541 gtattgactt ctttacttgg aacaacagct attgcagcat tgatcggaat tggttcaaca
     3601 atgctgttcg gactccaagg agctaaattc acacaaggag ctgcagaaac agcacgaatc
     3661 tctgaactgg caacacgtca ggaaacagtt gaaaacttat cgattccaca gcagatttta
     3721 tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta gtcgtgcaac aagtacgatt
     3781 ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc taggcgtgaa aagaaaagcg
     3841 ccaaaagaag cagcattctt ttcagattta gtcgacagtc tgtataaaat cattatgcgg
     3901 atcgtgacgc ttgttttgcg tttgacacct tatggagtat ttgctttgat gacaaacgtt
     3961 ttggcaacaa gtgattttgc agctatcgtc aatttaggta aatttgtcgt tgcttcttac
     4021 gtagcattgc ttgctgtttt gattgtccac tcactgattt tgattggtgc aaaagtcaat
     4081 ccaatcaact atttcaaaaa agcctttccg gtattgagct ttgcgtttac ttcacgttca
     4141 agtgctggag ctttgccatt gaatatcgag actcagacaa aagctttagg agtagatgat
     4201 gcaacagcta atttctcagc aagttttggt ttgtctattg gacaaaacgg ctgtgctggc
     4261 gtttatccag ctatgttggc aacgatcgtc gcaccagcag ctggagtgaa tgtctttagt
     4321 ttggaattta ttttgatgct tgtggcaatt gtaacaatca gttcttttgg tgtagctgga
     4381 gtcggtgggg gcgcaacatt tgcttcactg atcgttttag gggcaatgaa cttgccagta
     4441 gcaatcgttg ggttagttat ctctgttgaa ccattaatcg atatggcacg tacagcagta
     4501 aacgtcagcg gtagcatggt agctggtatc gtgacaagtg cacgcatcaa agatttagat
     4561 cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga atgcatagtc aaaaagaggt
     4621 tgtgatagaa gtggtcagct ttaagaaata agaagaagtt tcctaaaaat agtttctcaa
     4681 atttttgtga aatttcggct tatttccgag aagctacttc tgaaacaccg tgaataagag
     4741 gttgtgaaaa agctgccctg catcaagtaa taagaacagc caaacaaaaa tagttcctca
     4801 tattttacgt ttggttgagc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa
     4861 agaggtcgtg agcctgccgt ttcactctga gtcacaaaga gcaatttcaa aaaatagctt
     4921 ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt gtcaggagaa caccgtttac
     4981 cagaggttgt gacttgtttt tagtcacgac ctcttttttt atttattcca tataaataca
     5041 cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa caaccaactt atttattatt
     5101 aaagaaaagc gtatttaagt aggacgattc tgttgagagc tttcgtagat tttcccattt
     5161 tcctatggta aactgacaaa gttgagaatg ttagaacaga t
//
LOCUS       DAIGOK010000139         5064 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.141, whole genome shotgun sequence.
ACCESSION   DAIGOK010000139 DAIGOK010000000
VERSION     DAIGOK010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.141"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(<1..833)
                     /locus_tag="NOA01_002369"
     CDS             complement(<1..833)
                     /locus_tag="NOA01_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCI0633532.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            complement(847..1644)
                     /locus_tag="NOA01_002370"
     CDS             complement(847..1644)
                     /locus_tag="NOA01_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HCI0633533.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            complement(1677..1961)
                     /locus_tag="NOA01_002371"
     CDS             complement(1677..1961)
                     /locus_tag="NOA01_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCI0633534.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            complement(1958..2959)
                     /locus_tag="NOA01_002372"
     CDS             complement(1958..2959)
                     /locus_tag="NOA01_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HCI0633535.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            complement(2961..4028)
                     /gene="gnd"
                     /locus_tag="NOA01_002373"
     CDS             complement(2961..4028)
                     /gene="gnd"
                     /locus_tag="NOA01_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HCI0633536.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            4027..4875
                     /locus_tag="NOA01_002374"
     CDS             4027..4875
                     /locus_tag="NOA01_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCI0633537.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
BASE COUNT         1521 a         1096 c          834 g         1613 t
ORIGIN      
        1 taaaatccgt catcatagtc atcttcgtca tcaaaagatg ctgaattgtt attgcttgtg
       61 gcaggctgta cttgtttccc tttttgcgac tggacaaaga tcaaagcgat aataatccca
      121 aaaatggcat aagtaaccat cgtaatatct aaagatttca aaacaacact cataaaataa
      181 gcaaggaaga aaaagaccag ataatctttt cgaccgataa cgtatacagt tgtcgcgata
      241 ccgattgccg ctaaccctcc acctactact tcaagaatgt ggataacgac tgttccctct
      301 aaagctttga ttacatcagc gatcaatggc gcccctaaat ataaagcaat gaaaacaagc
      361 ggaacgaata ataaaaatga ggcaaccgtt ggataaagaa taattgatcg ggccatagct
      421 tttgtattca aatcttctac agctttgtct gtatatttac ctaaaaaagt attgatgaaa
      481 aaacgaaact gatacaagta acttccgagc aatccgactg gcaccgcaac ggcaatcgct
      541 gcttctggcg acagatcccc taataatgcg actggtactg cgatagctgc tgcaatagat
      601 ggttcagctg gcattgatcc tcctggagaa aaaaccccca tataaatcat ttgcaaagcg
      661 gctgtaacaa tcatcgcttg tgcaacttcc cccatgatca acccgacgac taatccagtc
      721 atcaaaggag aaaaacgtaa ggtcaacgtg gctcctccac ctaaccagcg ggacataacc
      781 gcggctaccc ataaagcaat caacaaactt tgtactacac ttaatgtttc catgtgtcct
      841 cctccttcag atctttctga tataaccttc cagttccatc attgactgta tcagtgattt
      901 ctgtgttacc tctataggaa tcaaatgcac tttctctttc gaatcaataa attcagccat
      961 tttatctatt atctgtttat ctttcggaaa taatcccatt tgttctagag aaaccggcag
     1021 ttctaattcc cgatacaaag gatataactc gtctattttt tcccagcgac cttctagtgc
     1081 cagttgatag aaaatcccat acgctacttt ttctccatgt agaaaacgat ggctttccgg
     1141 aatatattta ctgatagcgt catgcatagc atgtgctgcc gcatttcttg cgtatttatc
     1201 tcctaatcct ccaactaaac cagcaacagc aaagataatc tctgaaagct gtttgaactc
     1261 cgatgtcagt tttctttgtg ccatatcttg gattgcttta gcagaatgat tcaaaatctc
     1321 ttctttgcag atcttcgcag tatcttgtgc tagcttcaaa aatgattcat cttgcaaaaa
     1381 atcttgttct aagatgagct cagattcata ccattttgct agtgtatctg ctaacccggc
     1441 aatgaaatat ggaacagggg aatcaatcac taattgggga tctgttataa aaaaagcagc
     1501 ttgtctgtaa aagtgttctg ttttcccttc tgcttgtcca ttctccttat acatgacagc
     1561 aagaggtgtc catggagcac aattactagc taaagtaggg atgactccga aatcgattct
     1621 tgcaagattg gcactataac ccactagatc ggtcagtttt cctccgccaa cgccaactac
     1681 gaaatcgatt tcattttttt gatgattgta gctattcgtc tggcttcagc ataactacat
     1741 tctccagaat aacgatgata aaaaaattga atcgtttcgt cattcaaaaa tactaattta
     1801 ggtagcgcct tttcccatga taccgttccg tgggtgacca gtactctttt cgcgccgtat
     1861 gatttcaaga gatcagaaac ttttgaaata ccaccttctt catataaata aaattgaggg
     1921 cctgttttca cttttaaacc gttcaccatt ttcttcatca tccttttata tagttgctct
     1981 ttgtctggac agaacgttca ggaagaaggt gctgactgat ctgttcgaca tctctgacac
     2041 atttttcacc cattgcttct aaacattccc ttgtgtaggc cgatatatgt ggcgtaatca
     2101 ctacttgttc aaacgctaaa tacggatgat cttgacgtcc cggttcttct tctaaaacat
     2161 ctgtggcata tcctgcgatc tttccgcttt tcagaccatt aatcatcgct tcttcatcaa
     2221 ctagagcgcc tcttgcacta ttagatagat aaacattttc tttcatctga tcgacttctt
     2281 tatcagaaat catgtgatag ttctcttctg tcaaattcgc acataaacaa atgatatctg
     2341 attgttccag taattcttga agttctactt tttttgcacc aaagctttcg atgtataaat
     2401 ctgatttgta aggatcataa gctagaacat cacagcgaaa accgttacgc aaaatttcga
     2461 ttactccgct tccggtattt cctgcaccaa tcacaccaac tgttttgtta aacagcgtcc
     2521 taccgacgaa ctccgcccga tcttcccatc gatcgtcctt gactcttgct tgtgcttgtg
     2581 tcgtgcgtcg aagcatggac aacaaattag ttatattatt ttctgctaca gcatttcgtt
     2641 caactaaagc aggtatgata gatacgattg tctggtgttc ttttgctgct tttaaatcaa
     2701 tattgttgta accaatacca tgtcgggaaa tcaatagcag ttcatctttg ttttcaaaaa
     2761 aatctcgtcg aaaaaatggc gtaacacttg caataatgat attgaatcct ttaagtaact
     2821 ccgctagttc ttttcctgga atctcttgat caacagtaaa gaaatgaacg tctccaactg
     2881 actttaatct ttccaagtgg tgtggaaaga ctcttccgaa actgctggaa ttaacgattg
     2941 ctatatgata atctggcatt ttactcaccc ttttcaacta tttcatgtcc gccaaaccca
     3001 tttcttagtg ctgaaactac ttttgctgaa aaactatctt cttcctgtga aagattcctc
     3061 atgaacaaac tagttgcaat tactggaact ggaacagcca tttctagcgc tgtttcaacc
     3121 gtccattttg cttcacctga tgcagaaacg attccccgat aattacttaa attgggatgt
     3181 tcgtttaact gatcttccat gatttccatc agccagccgc gaatcaccga accatgattc
     3241 caaacacgtg cgacttgttc caagtcaaaa gcatactcac ttttttgtac aagttggaat
     3301 ccttcaccga ttgcttgcat cattccatat tcgattccat tatggatcat tttcaagaag
     3361 tgtccgcttc cggatgcacc agcatacaga tatcctcttt caattgctac atctttgaat
     3421 aattgttcca cggatagaaa agtttcttta tctcctccaa tcattaagca ggcttgatgt
     3481 cgtgctcctt cgattcctcc agaagtccca caatccaagt aaccgatttc atttttctga
     3541 aactcttctg ctcgacggat tgaatcttta taataagagt ttccgccttc aataataatg
     3601 tctcctggtc cgagttttgg aaaaagtttt cttaacatgt tttcggttat ctctccagca
     3661 ggaagcatta accagatgat ttttttctgc tcaaaagtag ctagaaattc agagatctct
     3721 tgataagcag gaatgtcatt ttctcttgcc tgactaacag ctgtacttga aatgtcgagt
     3781 ccttgtactt tgtgatggtg ctcttttaag tttaaagcta aattcaatcc catttttccc
     3841 aaaccaatta caccaatttc catgatagcg cctccatttc ttataccctc attatacaaa
     3901 aaaatttttt tgtaaataga ttttacaaaa atattttttg tttgattttt tcctactctc
     3961 cttgtatctt taacattaat gagaacggaa aaaatgataa tcttattatg aaaatgagga
     4021 attctcatga gcgtctccat aagactacaa atcaaaacaa tgtacaacga cttcagccca
     4081 aaagaacagg ctatcgccga ttatatttta gaaaatccaa gcaaagtaag tcatagtcct
     4141 attagcaatt tagcaagtga actgggcatt gctgactcta ctttttttca atttacaaaa
     4201 aaacttggtt ataatggatt taaagatttt aaaatggcca tgttgatcca agaaaatgat
     4261 ctatctgcta tttctatcca cgaaaatatt cagaaaacag atactgagct cactatggcg
     4321 caaaaggtgt tcgagtctag tatttcaact ttaaatgaca caaaaaagtt gttacaacaa
     4381 aaggatctaa aaaaagcggc tactattatc aatgaatcta aacgtgtcta ttttttcggt
     4441 gttggcggtt cagagattgt agcaactgac gcttatcata aatttttgcg ttcccctatc
     4501 gcaacttctc acagcacgga ttaccatatt caattgatgg aagcttcttt attgacggaa
     4561 aaagactgtg ctgttttgat ttctcacaca gggcaatcaa aagaaacaat ccatattgct
     4621 gaaaccgtaa gaaaaactgg agcaaaaaca atcgttatta ctagccaagc taattcttcc
     4681 cttgctaaac ttggcgatgt cgtttttatt tctatttctg aagaaacaga attccgatct
     4741 gaagctttag cttcccgtat ttctcaacta agcattttgg actctctata tgtgattttg
     4801 atgttttaca acgaaaaaga atcacgtcgt tctatcgcta aagttcgaca tactatttct
     4861 gaaatcaaag aatagcacca taacagaaaa agcgaaaaat ccataaaaca aaaaaagcca
     4921 ctaccaaata tttggtagtg gcttttttgg ttctgcaagg gtggtacaga acatcaatag
     4981 tcggtgggtt agaacgtatg tcatcgcatc tttggagaag gttgtattgg tattattcga
     5041 gttacttgtt agatgcgata acaa
//
LOCUS       DAIGOK010000140         5059 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.142, whole genome shotgun sequence.
ACCESSION   DAIGOK010000140 DAIGOK010000000
VERSION     DAIGOK010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.142"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(<1..365)
                     /locus_tag="NOA01_002375"
     CDS             complement(<1..365)
                     /locus_tag="NOA01_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HCI0633538.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHV"
     gene            complement(672..1769)
                     /locus_tag="NOA01_002376"
     CDS             complement(672..1769)
                     /locus_tag="NOA01_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HCI0633539.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            complement(1877..3904)
                     /locus_tag="NOA01_002377"
     CDS             complement(1877..3904)
                     /locus_tag="NOA01_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HCI0633540.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            complement(4049..4780)
                     /locus_tag="NOA01_002378"
     CDS             complement(4049..4780)
                     /locus_tag="NOA01_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HCI0633541.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
BASE COUNT         1427 a         1034 c          805 g         1793 t
ORIGIN      
        1 ctcacgtgat gctgtttagc aaaattttct actgagatat atttttcctg aaagaaagcc
       61 aataccattt tcatagataa agaatccgtt atataacgag ataacagagc tttgctagaa
      121 cattttccat taatttccag acatatatcc aattcactga cggttactcg caaatttttc
      181 tctagatcga acttttgtaa atcatgtttc agcttatcga tcgttttttt caagagaaat
      241 gtcgatagcg acaactcttc ttgaacattc gatattgact ctgataaatt tgagcttgct
      301 tctaaatatt taagtaatga ataacttctg tgatcttctt tgcttaaaaa aaactcataa
      361 ttcattagtc atcagctctt tctacgtata ttactttcca tgcattcacc aactagctgg
      421 ggataaaact gatttcctgg atccgagtca ctgttgtcag cgattcttgt ttgtataata
      481 tacatcgtcc tgtctttttt ttaacatttt ggagagcgtt ttatttggcc tttctcgcga
      541 tcaaacctgt tattagaatt atatcattta ctagtatgca tttttattaa taaaatggta
      601 tattttatat tgttataaaa agagcccaac catcctacaa taataaaaat ttactatttg
      661 ttatcttaac cttacttttc tttctcttct ttttttctca aaacaaacaa aagtatgcat
      721 aataaaataa acaataagaa aagtgaagct actaagatat aaatccaatt attgtctttt
      781 ttaatcgtaa catccgtcgt gttcaattcc ttggctactt ttccgtctat tgtaaaatct
      841 ttttcaaata cccattgatt ggtatagttg atcgtagtat ttcctttttt tctcgaaaat
      901 tttccatttt cattttcatt tcctaaaaca gtcattgtaa ggtgatattc acctggggtt
      961 agcggttgac catttaaagc agttggaaaa gaaaaatttg tattcggcgc aatctgcaat
     1021 ccttcttttt cctcttgata taaaacttca gaatgtcctt tttttgtaat ttttgtttca
     1081 ataactactt gattgatata ggttttttga tcattttgaa cgtttgctaa gatgacattt
     1141 cttgcattga tttgatctgg tttaacttca tgaagagtaa ggttcggagc aacttcgttc
     1201 agtgtctgtt gcatcaacaa agcaataata taggtgtatt cattctcaat cgataagctt
     1261 tgatccttag tttctgtttg gtccttttcc gtctggattt cttggaatgt gatcccacca
     1321 gccaatacac catcaaatcg ctcattcggc atcctgacat gaaaagcaac ggtttggaca
     1381 cttttaggtt ttagcgtcac tatctgtgga tagctcacat aatctaccag atcaaagatc
     1441 aggctcatgt ctttttcaat ctcgtttttt ccgtattcaa ccatcacatt tgaattggtg
     1501 gttgcgctat tcacagaaat atcgattttc acttcttttt ctgtatcatt tctcaattcc
     1561 gcttttactt ctacttcctg atccggatca agttgaagat cgaagtaagt ttttctcttg
     1621 tcgatttgat tctctgaagt tatcggcgtc actgaaaaat taaatgcact agctgacact
     1681 gatatattgt taaaaagtgc ccctaaaata actaggagtg ttataaataa ttgttgtttt
     1741 ggtctcaaga aaatcgctcc tttaatcata tgtaagagaa gactataaca aaatcttgtc
     1801 tctgtgggac acaagagcct atggtacctg tgcccttctt gaaactcaat aattttgtta
     1861 tattcttctc ttcctgttaa tttcctggga catctgcaag tatccaagat agtttcccct
     1921 caaaggttcc gattctttgg ttttctacag gaatatctat tctgattcca cgatgagctg
     1981 tagaatctaa aagagaggac aaatcgcttt cacccttttg atcactttcg aaaatcgtaa
     2041 cagcggatgt ccctataacc gttgtatttt cagaggtaac aaaggaaaga gcttctggaa
     2101 gctcaaaatc tccatttgta agaactttct cttgtttcaa ttgtaatttc caacttccaa
     2161 aagcccgttc atctataatt ttcaacccac ctgaaaactg tccgaattca cttaatggtc
     2221 tactggatat tttatgtttt ccaaaattga catattgagg agcagttgag agataaagtt
     2281 tgccttttct gtaataaaaa tagatatcct ctgtattttc ggagaagatg ccttctgtat
     2341 tcgctgaatc cgagtcaaaa acataccctt caatatcaat aggagtttct ttatacttct
     2401 ctcctatttt cccagtgatt gttttcattt cagcaatatc aatctgactg tctttatctt
     2461 tataataaac atggatatct atctccttca gctttgcgat ctttaggttt atttcattcg
     2521 attgctcaat caaattcccg ttttcaaggt attcaaaatg catagtgagt actttttcag
     2581 gaattacttc ctgcaaagtt gtctccaatt cgattattaa cgtatctcca gcaataaaat
     2641 aatctgtcag atttagcgtg actcgatttc cggttatcaa gatatctttg gcatcaattt
     2701 gatttccatt gatcaagata tgctcgggaa tagttaattc ttgtgggagg tcgatactag
     2761 tctgcatttt cttatattca agaaaatccc cctcgacctt caatgtattt ctgatagtta
     2821 tcttatcgtt tcctccagct tcagcgatgg ccccataatc actatcgttg acttttacat
     2881 cagctgttga tgtgacattc gatggaagtc gcgtgacagc gacagcattt tcttgaaaaa
     2941 aagtacctgt tgaagaagta aacccccaat ttacttctgt agaaccaaac tgcctaatga
     3001 gatcaggtat aatccgagaa gagtgaccga caccttccaa atcataagta agacttcttt
     3061 gctccgcgtc ccatttaatt attaacaagc gccatgtgcc attagaaaga aatgtaggag
     3121 ctaccgtcac gccagtatgc tctccagaat aattattatt atttggttta ggcgtgacaa
     3181 aagcgacatg tccatgccct ctattatcag cagttatctc tcgatccatt cgatcagaag
     3241 aaccactatt tttatacgta tcaaactcaa tcgagagcgc attcgcaatg taaggttggc
     3301 cagaacgagt agaatatgag ccaattccca tacctggcga tccaataact tgactgggat
     3361 tactagccat tctaggatcg ccagtcaatg taaaggtcat gccatctccc gctctatctc
     3421 gttgatttcc taagtataaa taagatttaa atgtaaaatc cgagagtaaa ctaatcttct
     3481 tatttgacca aatagctcct ctttgagacc tttctgctgg gttcaactgc agtaacttcc
     3541 cattttccaa tatggacgtg ctagtaccga taggtgtttg aaaaatttta tcgatagaaa
     3601 taatattggg atcgtcaggc agctcaaccg cagaaattgg tgcatcttct agtccttgtt
     3661 tgtccgaacc ctcagcttct acgttgtcag tggtctctcc actactcgaa atacttaaat
     3721 cctcactgct ttcttgtgtc tggttggatt ctattaaact gtcgaatgtc gaagtgagtt
     3781 tgctttcttc gtctcttcta tttccatgct ccaactgatt caattccaaa gagtacccta
     3841 tgatcggaaa gatattagtc actaagagac tgcttaaaat gagcacgatc ctcctctttt
     3901 tcaatatatc ttttcctcct gatcgttaaa ttcctagatg aactattcgt atccaaacaa
     3961 atttagccta gcctttgaca atttttgctc gattacctcg caagacaata tgactacgta
     4021 tttattttta acagttcttt tcagtaaact agttgttagg tgtatcttct aatgtccaaa
     4081 ctaatttagt ggaatattgt tgcgctaact tgacagattt accgggtacc gaaagctgta
     4141 ccgcatcttg attttcattt tcatttgcac caaatcgata gacccaagtc cccacacctg
     4201 ttcctgattt agcattaatt gctaacgtat tgtttcctcc cggtgtcaac gttaattcag
     4261 aattcaccgt ttgcggcgta tatgttggat caaccaaaga aacagcctgc cctttcgtaa
     4321 atttcagctg tgctccaact agttcatccc cactggcagt tttaaactgt tctggttgag
     4381 agacacttaa tttccaccca gataaggtac ttctcctatc tgtcacttgg acatatgtcg
     4441 gttttctaga cccatcgttc atcacatcag cagctgcata atacgtttta tccgcagtag
     4501 aaattttttg agtcccaaat ttgaattttg atccataatc aatcgataat gcccctcctg
     4561 ttccagggct tggaggatct gatggatcga caggtttatt gggatcagga ttctctggat
     4621 caacaggagg agttacaccc tcatctcctg cttcaaatgt aatcgttcct tctgtgttat
     4681 attcctttgg gtatacttca gttgcagaaa caaaagtcgt acttgctacc atgctgaata
     4741 acatcattgc tgctgttaat acgatttctt ttttcatcat tgacattcct ctttctttat
     4801 ttactaaaca tatcagtcga ttggattaat ccatagcaaa attctgtttt acgatactta
     4861 tgaaataaaa gaacatagac ctatatttgg tcctatgtat cgattagtta taaatgatta
     4921 tcagcccgat ttgatttcta gtttttattg caacttgatg caaacacaat ctagaaatac
     4981 agctgtcata gcctttaaaa taattcgtaa gtccccctcc tatctatcaa aatgtgactt
     5041 gactgcctag acttcaatc
//
LOCUS       DAIGOK010000141         5018 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.143, whole genome shotgun sequence.
ACCESSION   DAIGOK010000141 DAIGOK010000000
VERSION     DAIGOK010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.143"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..863
                     /locus_tag="NOA01_002379"
     CDS             <1..863
                     /locus_tag="NOA01_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0633542.1"
                     /translation="QGLQKKFGKFQALKDVSFTVNAGEVVGFIGPNGAGKSTTIRTLL
                     GIINRDEGDVQIFGKDVWKDSLEIHKRISYVPGDVALWGSLTGGEIIDLFIKLHGGGS
                     KAKRDYLIKRFELDPKKKAKGYSKGNRQKVGLIAALSVESDLYILDEPTSGLDPLMEA
                     VFQEEVEKIKNDGKAILLSSHILSEVERLADKVAIIRRGEVVETGTLDELRHLTRSTV
                     TLVTEGDIEKLATLSGVHDFVQKDGKATFSADNEAMNTILTEATKLGVTKIESVPPTL
                     EDLFMRHYEG"
     gene            887..2500
                     /locus_tag="NOA01_002380"
     CDS             887..2500
                     /locus_tag="NOA01_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HCI0633543.1"
                     /translation="MNEKFARWNVLFIQYVKRDWKKIIVWVLGLGLFSGAFVPAFEEI
                     AKGQGLLGMFETMQNPAMISMVGPTPIKIGTDYTLGAMYAQEMLLFCGLFAMIISALH
                     VVSHTRKEEELGLTELVRSFRVGRQANSLAVISEMLLINLLLGLLIGGLMMSFGVKTI
                     DAEGAFLFGGSIALAGIIGGVLALVMSQIMATSTGATGSTLSLIGLLYIVRAGTDVSN
                     LDLSMFNPMGWIYLTYPFTENNWLPLLFALIFSLVFTVLAFVLEEHRDMGAGYLPERE
                     GRATAKKSLLSVPGLFFKINKGVMIGWLIAFVVMGAAYGSIYGDMQVFLGGNELMKQM
                     FTQSGVSIEESFTATIMMVMIGLVTILPIAVVNKLFAEETRLHLSQLYVTKITRGQLY
                     WTTIFLAIFAGVVGIGLASAGLGGTAISAMKNESTMDLTDFLAAGYNFLPSILFYIGL
                     AALALGWLPKFGKVIYAYLGYSFALNYFGGILDLPDWFSKTAIQSWIPRLPMEKFDGT
                     IFAVITVISIVFLFVGYLGYKRRDMVEGA"
     gene            complement(2572..3084)
                     /locus_tag="NOA01_002381"
                     /pseudo
     CDS             complement(2572..3084)
                     /locus_tag="NOA01_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            3347..3463
                     /locus_tag="NOA01_002382"
     CDS             3347..3463
                     /locus_tag="NOA01_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta-toxin"
                     /protein_id="HCI0633544.1"
                     /translation="MVQNQHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI"
     gene            complement(3726..4463)
                     /gene="erm(B)"
                     /locus_tag="NOA01_002383"
     CDS             complement(3726..4463)
                     /gene="erm(B)"
                     /locus_tag="NOA01_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HCI0633545.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(4588..4671)
                     /locus_tag="NOA01_002384"
     CDS             complement(4588..4671)
                     /locus_tag="NOA01_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HCI0633546.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
BASE COUNT         1547 a          869 c          992 g         1610 t
ORIGIN      
        1 tacaaggctt gcaaaaaaaa tttggtaaat tccaggcgtt gaaagatgtc tcattcacag
       61 taaacgccgg tgaagttgtt ggttttatcg gaccaaatgg agcaggaaag tcaacgacga
      121 tccgtacact gctaggaatc atcaacagag acgaaggaga tgtccaaata ttcggaaaag
      181 atgtttggaa agatagtcta gaaatccata aacgaatttc gtatgttcct ggagatgttg
      241 ctctttgggg cagcctgact ggtggagaga tcattgatct atttatcaaa cttcatggcg
      301 gcgggagcaa agcaaagcgc gattatttaa tcaaacgatt tgaacttgat ccaaagaaaa
      361 aagccaaagg ttactctaaa ggaaatcgtc aaaaagtcgg tttgattgct gcactttcag
      421 ttgaatctga tctgtatatt ttagatgaac cgacttcagg actagatcca ttgatggaag
      481 cagtattcca agaagaagta gaaaaaatca aaaatgatgg caaagcgatt ctattatctt
      541 cacatatttt aagtgaagtt gaacgattag cagataaagt agcaatcatt cgacgtggag
      601 aagtagttga aacaggtaca ttagatgaat tgcgtcattt gactcgctca acagttacat
      661 tggtgacaga aggcgatatt gagaaacttg cgacgctctc tggcgtgcat gattttgttc
      721 aaaaagacgg caaagcaact ttttctgctg acaatgaagc gatgaatacg attctgaccg
      781 aggcaaccaa attaggtgtg acaaaaatcg aatctgtacc gccaacgctt gaagatctat
      841 tcatgcgtca ttacgaaggc tgattgtcgg aacggaggaa aagaaaatga atgaaaaatt
      901 tgcccgttgg aacgtattgt tcattcaata cgtgaaacgc gattggaaaa aaataattgt
      961 ttgggtttta ggtttgggtt tgttctcagg agcatttgta ccagcatttg aagagattgc
     1021 taaaggacaa ggtcttttag ggatgtttga aacgatgcaa aatccagcga tgatctcgat
     1081 ggttggacct acaccaatca aaataggtac ggattatact ttaggagcga tgtatgctca
     1141 agagatgttg ctgttttgcg gattgttcgc aatgattatc tcagcacttc atgtggtgag
     1201 ccacacgcga aaagaagaag aattaggttt gactgaattg gttcgctcat ttcgagtagg
     1261 acgacaagcc aattcattag ctgttatcag tgagatgctg ttgatcaatc ttttattagg
     1321 tcttttaatc ggcggactca tgatgagttt tggtgtaaaa acgattgatg ccgaaggagc
     1381 tttcttgttc ggaggatcaa ttgcattggc gggaattatc ggtggtgtat tggcacttgt
     1441 gatgtcgcag attatggcga cttctactgg agcaaccggc tcgacattaa gtcttatagg
     1501 acttttgtat atcgtgcgcg ctggaacaga tgtgtctaat cttgatttat caatgttcaa
     1561 tccaatggga tggatttact tgacctatcc tttcacagaa aataactggc taccattatt
     1621 atttgctttg atttttagtc ttgtttttac cgtacttgcg tttgtgttgg aagaacatcg
     1681 cgacatgggc gcaggttatc ttcctgaacg agaaggacgt gcgacggcga agaaatcact
     1741 actttctgta cctggtttgt ttttcaagat taataaagga gtaatgattg gttggctgat
     1801 cgcatttgtc gttatgggag ctgcgtatgg ctccatttat ggagacatgc aagtctttct
     1861 tggcggaaat gaactgatga aacaaatgtt cactcaatct ggcgtttcca ttgaagaatc
     1921 ctttacggca acgatcatga tggtaatgat tggattagtc acaatcttgc caatcgcggt
     1981 ggtcaataaa ttatttgcag aagaaacaag actgcatctg agtcaactgt atgtaacgaa
     2041 gattacgcga ggccaattat attggacaac gatattttta gctatttttg ctggagtcgt
     2101 aggtattggc ttagcatcag cgggattagg tggaacggcg atttctgcga tgaaaaatga
     2161 atcgactatg gatctgaccg atttcttagc tgctggatac aattttctcc cttccatctt
     2221 attttatatt ggtttggctg ctttagcgtt aggctggttg ccaaaatttg gaaaagtaat
     2281 ctatgcttat ctaggctatt cctttgcttt gaattatttc ggcggaatct tagatttgcc
     2341 ggattggttc tcaaaaactg caattcaaag ttggattcca cgcttaccga tggaaaaatt
     2401 tgatggaacg atttttgcag taattactgt tatcagtatc gtcttcttat ttgtcggcta
     2461 tttaggatac aaacgccgtg atatggtaga aggcgcttaa aaattgaatt tctaaaaaac
     2521 cgttcatatt tacgaaagtc gatttgagcg gtttttttat ggcttcaatt gtcaaattaa
     2581 gtgcaacgat tcattaaaaa atacttcata aggggttttc caatttagac atttccgtgg
     2641 tcgcgtgttt aatttattcg cccattcttg aatttgacta tcgaaaatac ctgaaatatc
     2701 ttcacctttg ggtgaatatt ctcgtaataa accattagta ttttcgtttg taccacgttg
     2761 ccatggagca tgtggatctg gaaaatacac tttagtgcca tttaattcta gcgttattcg
     2821 actatgttgg gagaattctt ttcctctatc gggtgtaacg gttttacatt tatcttctcc
     2881 tacatttctt agtagattga tcatacaatc aaccacattt ttagagattt tcttttcaac
     2941 cttaccaagt agggagaaac gagatctacg atcgactaga gttacaatac gagccctacc
     3001 tgttttaccg gctacagtat cagcttccca attaccaatc tcaaggcgct cattggcagc
     3061 agatggcctt tcatggatct tattgtatgc agatgatcca atgacagcta gggcaccacc
     3121 aaaacaagcg catgatattg ttgtcaaaaa cttaccgacc aatttagaaa cccttcataa
     3181 aacgggctta tttagcgata taaggcttta caatagagaa ggagtgaaac tctattcaag
     3241 cttagaaacg ccttccatta gtccgaaaga gaccttagaa agagaattaa atcgtaaagt
     3301 atcagggaaa gaaattcaac cgactttgga gagaatagag caaaaaatgg ttcaaaatca
     3361 acaccaagaa acccctgaat ttaaagcaat tcaacaaaaa ttggaaagct tgcagccacc
     3421 tacaccacca atacccaaaa cacctaaact tccaggtatt taaaccttaa aaaaggaaaa
     3481 gagtagttac caaaaaacgg taactactct ttttttataa aaacatttcc tcatgttggg
     3541 gtcccgagcg cctacgagga atttgtatcg ataagaaata gatttaaaaa tttcgctgtt
     3601 tttttctaat ttcattttga ggtgaattaa ttggtagtta ttagaggtgc cctataccta
     3661 ataatttatc tacattccct ttagtaacgt gtaactttcc aaatttacaa aagcgactca
     3721 tagaattatt tcctcccgtt aaataataga taactattaa aaatagacaa tacttgctca
     3781 taagtaacgg tacttaaatt gtttactttg gcgtgtttca ttgcttgatg aaactgattt
     3841 ttagtaaaca gttgacgata ttctcgattg acccattttg aaacaaagta cgtatatagc
     3901 ttccaatatt tatctggaac atctgtggta tggcgggtaa gttttattaa gacactgttt
     3961 acttttggtt taggatgaaa gcattccgct ggcagcttaa gcaattgctg aatcgagact
     4021 tgagtgtgca agagcaaccc tagtgttcgg tgaatatcca aggtacgctt gtagaatcct
     4081 tcttcaacaa tcagatagat gtcagacgca tggctttcaa aaaccacttt tttaataatt
     4141 tgtgtgctta aatggtaagg aatactccca acaattttat acctctgttt gttagggaat
     4201 tgaaactgta gaatatcttg gtgaattaaa gtgacacgag tattcagttt taatttttct
     4261 gacgataagt tgaatagatg actgtctaat tcaatagacg ttacctgttt acttatttta
     4321 gccagtttcg tcgttaaatg ccctttacct gttccaattt cgtaaacggt atcggtttct
     4381 tttaaattca attgttttat tatttggttg agtacttttt cactcgttaa aaagttttga
     4441 gaatatttta tatttttgtt catgtaatca ctcctgaagt gattacatct ataaacaaat
     4501 acagaagtta aacgatttgt ttgtaatttt agttatctgt ttaaaaagtc ataagattag
     4561 tcactggtag gaattaatct aacgtattta tttatctgcg taatcactgt ttttagtctg
     4621 tttcaaaaca gtagatgttt tatctacata acgcatttgg aataccaaca tgacgaatcc
     4681 ctccttctta attacaaatt tttagcatct aatttaactt caattcctat tatacaaaat
     4741 ttttaatgca ctatcaacac actcttaagt ttgcttctaa gtcttatttc cataacttta
     4801 gggttaacca tacgcaagac caatcactct cggacaatac tcatgatttt aatgataaaa
     4861 tccatgttaa acccatagat aagaaataca cctgcaataa ccgttacctg tttgtgccaa
     4921 tttaaaaatg caccactttt ttataattaa aacggttgaa aactgtacca ctaataactc
     4981 acaatagaga gatgtcaccg tcaagttaaa tgtacaaa
//
LOCUS       DAIGOK010000142         4980 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.144, whole genome shotgun sequence.
ACCESSION   DAIGOK010000142 DAIGOK010000000
VERSION     DAIGOK010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.144"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(316..2208)
                     /locus_tag="NOA01_002385"
     CDS             complement(316..2208)
                     /locus_tag="NOA01_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0633547.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2205..3932)
                     /locus_tag="NOA01_002386"
     CDS             complement(2205..3932)
                     /locus_tag="NOA01_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0633548.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3948..4598)
                     /locus_tag="NOA01_002387"
     CDS             complement(3948..4598)
                     /locus_tag="NOA01_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HCI0633549.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4802..>4980
                     /locus_tag="NOA01_002388"
     CDS             4802..>4980
                     /locus_tag="NOA01_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCI0633550.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLY"
BASE COUNT         1427 a         1053 c          897 g         1603 t
ORIGIN      
        1 taccaagagg ttgtgagcct gccgtttaac tccgagtcac aaggagcaat ttcaaaaaac
       61 agcttctcag gtttgttgac tattgtgact tgtgaccgag gagttggcag gagaacaccg
      121 tttaaggaag aggttgtaaa atcagcggtt tgacctgagt attagagata caaaacggaa
      181 aaatagcttc tcatattttg ccgttttttg agctaatacc gaaggacagc tgattgaaca
      241 ccgtttattc ggaaaaaggg actgtgacac gacttttgtc acagccccta aaaaaaataa
      301 taaatttgta ctattctatg aagcttcttc ttcagaaaac tgactgttat aaagatctgc
      361 atagaatccg tctttttcca tcaatgtatc gtggtttcct gtttctacga tcgaaccatg
      421 attcatcaca ataatgttgt ctgcatcgcg tattgtagac aagcggtgag caacaacgaa
      481 acttgtccgg ttttccaata ggcggttcat cgctttttga atcagtattt ctgttcgtgt
      541 atctacacta gatgttgctt catcaaggat caggacatct ggatttgcta agaatgcccg
      601 cgcaattgtg attagctgac gctgcccttg cgaaatattg cttgcatcct cgtttaatac
      661 agtgttatat ccttctggca atttacgaac aaaatcgtct acgtgagcag ctttagcagc
      721 ctcaatgatt tgttcgtctg ttgcatcgtc gttcccatat ttgatattgt catagatact
      781 tcctgtaaac agccaagtat cttgcagtac cattgaaaac tgtgcgcgca attcatcacg
      841 tgaaagatca cgggtatcta caccatcgta acgaatacta cctccagaaa catcgtagaa
      901 ccgttcaaga aggttgatca gtgtcgtctt ccctgcacca gtcggaccta caatagcgac
      961 catctcacca ggtttgacat ccaagttaaa atcagtcatt agcaagttat ctggtgtgta
     1021 gccgaattga acattttcaa aacgaacttt gtatggtgta tctttttctt ctggaaggcc
     1081 agatttttca ttggacatct cttcttcatc caacacttca aatacccgtt cggctgaagc
     1141 aactgttgat tggatagtgt tcatcaaact tgcaagttga gtgattggtt gagagaactg
     1201 tgttgtatat tgcaagaacg cttgaacatc ccctaatgtc atgttcccat ttgcaacttt
     1261 tactccgcct agtacagcga cgaacacata gcctagattt ttaacgaaat tcatcaacgg
     1321 catgatgatt gccgaaataa attgagcttt ccaaccagcg gcatacagat tctgattttc
     1381 tttttcaaat acttcctggt ctttttcagt atgattgaat gttttaacaa tggtatgacc
     1441 accatatgtt tcttctactt ggttattcaa taaacctaaa cttttttgct gatctgcgaa
     1501 aaacacttgt gatttcggcg ctacgatcat cacgatgatc aagcttaaag ggatcatcaa
     1561 taacgcgact aacgtcaatt gccagctgat tgaaagcatc atcacgatga ccccaatcaa
     1621 tgtgactgca ctagttacaa attgagtcaa attttgctgc agcgtgcttg cgatgttatc
     1681 catatcattg attgctcgag acatgatatc cccatttgta tgtgtatcat aataggaaat
     1741 cggtacacga ttcattttgg cttccaaatc atgacgtaat tcataaactg ttcgttggga
     1801 aacacgtgtc ataataaatt gctgtaaaaa gttgaataca gcagaaatca aatacatcac
     1861 gattactgtc gcaatgatct gcgcgatttt atcaaagtct atcggaaatt ttgttacttg
     1921 ttggccttgt tgcatctgtt gagcgccagc cattacacct ttgaatattt ctgtggttgc
     1981 tttccccaag acctttggtg tttggatttg gaagatcacg gctgctattg ctagtacaaa
     2041 tacagcgatg atcgcaacca tacgtttaga catatagcca aacaatcggc gtaccgttcc
     2101 ccagaaattt ttaggctttt caccttttac accgagattt ctgccaggtc caccatgtcc
     2161 tggaccgcct gctggtccat gagagggacg tgtgtttgtt ttactcatgc taagtcctcc
     2221 tctcttagtt gtgaatgaac gatttcttga taagtttcgt ttgttgctaa tagttcttca
     2281 tgtgttcctt tgccaaccag ttttccttca tctagcacaa ggatcatatc tgcatccatt
     2341 actgtactta ctcgttgagc aactaaaacg gtgatacttt ctttcatatt cgttttcaat
     2401 gcttggcgaa gattggcatc cgttttgaaa tccaacgctg agaaggaatc atcaaacaca
     2461 tagatatctg ctgttttgac taatgcccgt gcaattgcca gacgctgtct ttgtccacca
     2521 gagaagtttc ctcctccttg ttccacatgg ctatctaagc catcttccat ctctgaaaca
     2581 aaatctttag cttgtgcgat ttctaatgct ttccaaatca tttcatctgt tgcatccggc
     2641 ttaccatact gcatattgtc tcggatcgtt ccagagaaca gaactgcttt ttgaggaaca
     2701 aagcccatta attctcgcaa attatattga gtcatgtcac taatgtccgt tccatttatt
     2761 tggatcgaac cagattcgat gtcatataat cgagggatca aattgactaa ggtcgtttta
     2821 cctgaaccag tcccccctat gattgcgacg atctcgcccg acttggcttg gaaatcaata
     2881 tcttccaatg caagtttttc tgctccatga tatcgataat tgacatgatt gaaggccaat
     2941 gttgcgtttt ttccagcaaa agaaactgtt ttaggatttt cagggtcatg gataccgatt
     3001 ttttcatcca acacttcatt aatacggtca gctgaagctt gtgcacgagg caccatgata
     3061 aaaatagcgg aaagcatcat aaagctgatc aagatctgca tcgcataagt cataaatgca
     3121 atcagattac cgacttccaa agtcatatct gctatgtagt gtccaccaaa ccaagtgatt
     3181 gctacgtttg taccgctcat gatcaatgtc atcattggca tcatcaatgc gacgatcgta
     3241 ttaactttga ttgccgtatt tgtataatct ttattcgcta aatcaaaacg attttcttca
     3301 aagcgtgtct tatcgaatgc tcgaatcact ctgacccctg tcaatccttc tcgaaatacc
     3361 aggtttaaac ggtcagtttt cttttgcata cttttgaata atggaacagc aaagtacatg
     3421 attccgccga tcaaaataat cattactggg atgacaacta agaagatttt tgtcaactga
     3481 tgatcttttt gataagcaag tatgctcgcg ccaatcaacg tgattggtgc attgatcatc
     3541 atacgtaaaa acatttgtgt aaccatttgg atttgagtca catcattggt cgttcttgtg
     3601 atcaaagagg ccgttccata tttatcaaac gcatcttttg tcagactctc tgatttttta
     3661 tagacatccg tacgcaattg cttacccagt ttctgtgatt cacgagtagc aaaaaatgta
     3721 tttgcgatag cagctaagat actgatcaaa gaaaagccga tcatcacaaa acctgtatgc
     3781 caaatataat cgacatctcc ttgcgctacc cctttatcaa taatattgga ggtaagtgtc
     3841 ggcaaatata aatctgccag tacttggatc accatgaaaa ctgcagctgc aattgctgaa
     3901 gtcaaggaga tccgttttac gatttttagc atctagttcc cttctttcta tttatatact
     3961 ccgtttttca gccaagaaag tttcaaattg aattcactga ttgcttgctc tactgagtaa
     4021 tcttcagatg ttgaaaataa gcgatacgaa tgcgcaattg ttgtaaatac agtattcatc
     4081 actaattgga tcagtaattc caattcatga tcatttttta ctttcagctt cgtccgatcg
     4141 atcaattcaa gcaactcttt tgcatcttgt ttgtgttctt tatgtggatc gtgaggcgtt
     4201 ttttggcgac tcgtatgagg agcgactttt cttgaggcac ggtaatccat gttcataaac
     4261 aagttacgat aaaaagaagc attctcgcct tccaaaatct cataaatcat tttagaaaaa
     4321 taggattcga atcctttgaa tagatcacca tcggattctt tcattagttg taacatatct
     4381 ttagagctgt cacgtctcat tgtctcaaag taataaaaat aaagatcctc tttatcttca
     4441 aaatactgat agaaactgcc tctagggata tccgctaatt tgacgatatt agcaatcgaa
     4501 gcttctttta agggtactct agaaaactcg attctcgcag cctcgattag tcttttttgt
     4561 ttttctttag gcaaatggaa aaatgtctgt gtcggcatat ggattctcct ttctttcaag
     4621 atgacacatt gtcatatgac gctatgtcat tataacacct cctacctgtt ttgcaagaca
     4681 tttgcatagt ttttttcatt atcatccgat acattaaaaa agtgataaga ggaatccttc
     4741 catttgaatt aatgagagtt gcccattaaa atagccacat actaaacaaa ggatgagata
     4801 aatggcccaa tcaattagtg tccaagactt caataaattg cctctcgatg ctgacagcat
     4861 cgttttagat acaagaaata ccttagatta cacaaaaaac cacttaagcc actcgctttc
     4921 tatccccgct gctatcttgc caagacaatt aaaaaatcta gatccaaaga aactttatta
//
LOCUS       DAIGOK010000143         4976 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.145, whole genome shotgun sequence.
ACCESSION   DAIGOK010000143 DAIGOK010000000
VERSION     DAIGOK010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.145"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            329..1252
                     /gene="truB"
                     /locus_tag="NOA01_002389"
     CDS             329..1252
                     /gene="truB"
                     /locus_tag="NOA01_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCI0633551.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1256..2197
                     /gene="ribF"
                     /locus_tag="NOA01_002390"
     CDS             1256..2197
                     /gene="ribF"
                     /locus_tag="NOA01_002390"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HCI0633552.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2592..3377
                     /locus_tag="NOA01_002391"
     CDS             2592..3377
                     /locus_tag="NOA01_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCI0633553.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3390..4241
                     /locus_tag="NOA01_002392"
     CDS             3390..4241
                     /locus_tag="NOA01_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HCI0633554.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4365..4817)
                     /locus_tag="NOA01_002393"
     CDS             complement(4365..4817)
                     /locus_tag="NOA01_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCI0633555.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
BASE COUNT         1683 a          815 c         1038 g         1440 t
ORIGIN      
        1 attaagaaac tgaaaaaata aataaaaaaa aacggaaaaa agactggaag aatggacagt
       61 cttttttttc gtttttggga agtgaaaacc aaactattta cctgtaagcc acccataaat
      121 ccaatcaaat ggtgcaaaga aaaaatcttc cattctattc cctcctttaa aaaaaacata
      181 acatgatttc tatttacgtg agcatgaatt ttgttacgtt tttattgccg aaaaataaaa
      241 accgttcatt atgatgtcaa attcgtcagt aattgatgca atgtgttata ataattcagt
      301 taaatcaaaa atgaaatgga gaaaagacat ggacgggtta ttaactttat ggaaagaacg
      361 cggaatgaca agtcatgatt gcgtattcaa attaagaaaa atcttacata cgaaaaaaat
      421 cggacatggg ggtacgcttg acccagacgt agacggtgtg ttgcccattt gtattggaaa
      481 agccacaaaa gttatcgaat atttgacaga ttctgggaaa atctatgtag gcgagataac
      541 tctaggtttc agtacaacga cggaagatcg atccggagaa cgggtagaaa caaaaccagt
      601 agatatgtct ttatctgaag aaaaaataga tgaagtgatg gcttcattta ttggtgagat
      661 cacccagata ccaccaatgt actcagcggt aaaagtcaac ggtaagcggt tatatgaata
      721 tgcccgcaat aatgaaacag tggagcgtcc tgttagaaag gctcagattt accgttttat
      781 ccggacaagt gaattgacaa gagacgaagt gacaggaacc atttcttggc gcttcgaagt
      841 agaatgcggg aaaggaacct atgtccgcac attagctgta gatacaggca agaaacttgg
      901 ttaccctgcg cacatgtctg atctgacccg aacagcaagt gccggtatgt cccagaaaga
      961 tgctctgaca ttagaagaag tagctcaagc tgtgaaaaat gggacaatcg atgattgttt
     1021 gctgccaata gaaaaaggag tgaaacgatt caaacgagta gatattactg atgaagtatg
     1081 gcagaaagta aaaaacggta tgagacttaa ttatactgta tttgcgctcc cagaaatgcc
     1141 gtcagaagaa attgctttgt tttataaagg caaagttgtc agtatctatc agccaaaccc
     1201 gaaagaaaaa aacaaattaa aacctagtaa agtattgaga aatgaggaat aacgaatgaa
     1261 tatcatcaag atcagacatc cttaccaatc agaacaaatt ccagaagacg atgtcgtttt
     1321 agtactagga ttttttgacg gcgtgcatat tggacaccag aaagtcataa agacaggtat
     1381 ggaaattgca caaaaagaag gattgaaatt agcattgatg actttcaatc agcatccatc
     1441 gattgtcttt aaaaagatcg atcctagcag cgtgaaatat ttgactagcc ttaagcaaaa
     1501 agaagaaaaa atggccgctt tgggtataga ttatttatat gaagttgatt ttacttcttc
     1561 ttttgctcat ttagcaccgc aggaatttgt tgatcaatac atcgtagggt tacatgcaaa
     1621 atatgctgtt tccggttttg attatacgta tggaccaaaa gacattgcag atgtcgctca
     1681 ttttcctgaa tatgccaaag gaagattcaa aatcgtcaca gtttctaaag aagaagaaca
     1741 aggtgaaaaa atcagctcta cccgtattcg aggattacta gacagcggac aagtaaaaga
     1801 agcagcaaaa ctattaggtt gcacatatga ggtagaaggg gtagttgtcc atggcgatgc
     1861 acgaggcaga ctactaggat tccctacagc aaatgtcaaa gtaaagagca cagttcattt
     1921 gcctaaagaa ggtgtttatg tatcggaaat aaaaattggg gaaatttggt atccagccat
     1981 gggttcaatt ggacataatg atacattcgg tgaaggacgg gagctgactg tagaattgta
     2041 tattttggac tttaatcaag atatctatgg tgaacatgtc gcagtacgct ggaatcaatt
     2101 tttaagagat caagtgaaat tttctagtgt agaggaattg attgaacaac tcaaagagga
     2161 tgaacaagca acagctgaat atttttctca gcaataaaac attatgcata aaatctcttg
     2221 tttatgtata actagcaatt tcaataaaat gggtgataaa taagcccagg aaaggggctg
     2281 tgacaaaagt cttgtcacag cctttttttc caaataaatg gtttttcaga agtagcttct
     2341 tcggaaataa gccgaaattt cacaaaaatt tgagaagtaa tttttgtaaa ttacttctta
     2401 tttcttgaag ctggccactt ctgtcacaac ctctttcttt atagaaataa aaggacacta
     2461 gcaattcatg aaaacgattg ctaaagcgta tcatttgctt tagactatat atcttggtgt
     2521 acactcactt taagacggaa ataaaagtta tccgtaataa caggtttagg tatgaaaaag
     2581 gagtgggtac tatgtataag gaattagaaa acaaagtcgc agtagtaact ggtggctcaa
     2641 aggggattgg aacagcgatt gctcaacgat tcgggaaaga aaaaatgaat gtagttgtca
     2701 actataactc ggataaagaa ggagcagaaa aagcagtcaa agcaatcgaa gaggcaggcg
     2761 gaaaagcagt ctctgtacaa gcagatgtca gttctgaaga aggaatacag cgtttacttg
     2821 atacggcttt agaaaatttt gggacactgg acgtttggat taataatgct gggatggaaa
     2881 atcagcatcc aacccatgaa cttagccttg aagattggga aagagtatta aaagtcaatt
     2941 tgactggcgt ttttatcgga acgaaagcgg ctttaaatta ctttacagag aatgataaaa
     3001 aaggaaatat catcaatatg tcttctgttc atgaacaaat cccatggcca acgtttgctc
     3061 attacgctgc ttcaaaaggc ggagtaaaac tattcaccga aacggtagca atggagtacg
     3121 caccaaaggg aatcagaatc aatagtatag gacctggtgc tatccgtaca cctattaatg
     3181 cggcaaaatt tgatgatcct gaacaaaaag aactcttgga aagtatgatc ccactaggac
     3241 gcgtaggtga accaaaagaa atagcggcga cagctgcatg gttggcttca gacgaatcca
     3301 gttatgttac aggtattaca ctatttgcag atggtgggat gactttatat ccttctttcc
     3361 aaggtggaaa aggataaggg tgaataaaaa tgggtttatt gattgcattg atcccggcag
     3421 ttgggtgggg gatccagcca ctgattttga aaaagattgg tggcagacca acaaatgaga
     3481 tattaggaac tggtattgga gccgtgctcg ttggcttact tgttcaatta ttcgcttctc
     3541 cagctggtat ttcagtgact acatttttga ttagtttact ttctggtgca ttctgggtca
     3601 tcggacaaac aggacagtat acatctttca acttgattgg tgtttctaaa acgatgccaa
     3661 tttcgactgc attacagtta gtcggaacct cattgattgg tgtcttcgct tttggagaat
     3721 ggtctggaag tatgggaaaa atcattggtg cagcagcaat cattcttctt gtatttggtt
     3781 ctgctttaac ggcaatcagt gatgatcctg gaaaaaaagg cggtatcaca aaaggaatca
     3841 gtctattagc atcaacaagt attggttatt gggtttacag tgcattgccc aaactagttg
     3901 atgcaagcgg actttccatt ttctttccgc aaatgatcgg tgtcttttta ggggcagtga
     3961 tttatgtgat tttccgaaaa ccaaccgcat tttctgaaga caagagttgg aaagcaacaa
     4021 tagttggagc catcttcagt gtgtcagcat tggcttatat tttttcagca aatgaaaatg
     4081 gggttgctac tgcatatatc attacacagc taaacgtagt tatctctaca ttaggcggta
     4141 tgtacatcct gcatgaaaat aagacaccaa aagaaaaaaa attaacgatt atcggattat
     4201 tgctgatcgt agtaggaagc atggtcacgg tctttatttg aggataaaga tggtaaaatc
     4261 tcatttatta aaaaaatatt tgtttgaacc aaacagtaag tacttataaa aaaatagcgc
     4321 tagaatttcc aacatctctg gaatttctag cgctttttaa ttgtttaaga tacaagtgaa
     4381 atatctgtct gcatcaataa tttttcattg atccattgac taaaatttac gacatgattg
     4441 aagttaccac cgattagtat ctccgcatga ttgtctttat aaagatcgta ttgtaaaaga
     4501 cggtcttttt tatttgcgta aacatcgtta tggtactgga ctaattgttc tcggatttga
     4561 ttgattgctg taaaatcacg atactgaagg ttgaaacgca tcgtgattgg tgcatttagc
     4621 atgaatcgct gcatttcttt tgctgcttcc tctgcttgat ctccgtatag gaactcaaaa
     4681 agattacgtt cagaaaattt ccattgcccg ccaacctttg ttccttttag ttttccttca
     4741 tgaagataat ttctgataga acgtgtagta acacctaatt gttcggctaa ttcatttact
     4801 gagtaaaaat ctttcaatgt tcaccctcct atgtgaatgg ttctactgac ttagttgata
     4861 tttgcttaat cacgaaatgt agaaagaacg attttaatta aggtcattgt aaaaatcaag
     4921 tcagaaaatt ctaacttttc tttctctaaa gaaattcgtt ctctcctaaa gtgata
//
LOCUS       DAIGOK010000144         4823 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.146, whole genome shotgun sequence.
ACCESSION   DAIGOK010000144 DAIGOK010000000
VERSION     DAIGOK010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4823
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.146"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            98..898
                     /locus_tag="NOA01_002394"
     CDS             98..898
                     /locus_tag="NOA01_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCI0633556.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            917..2785
                     /locus_tag="NOA01_002395"
     CDS             917..2785
                     /locus_tag="NOA01_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCI0633557.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPTNRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            2778..3269
                     /locus_tag="NOA01_002396"
     CDS             2778..3269
                     /locus_tag="NOA01_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HCI0633558.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            3257..3811
                     /gene="srlA"
                     /locus_tag="NOA01_002397"
     CDS             3257..3811
                     /gene="srlA"
                     /locus_tag="NOA01_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCI0633559.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            3829..>4823
                     /locus_tag="NOA01_002398"
     CDS             3829..>4823
                     /locus_tag="NOA01_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCI0633560.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         1678 a          756 c         1021 g         1368 t
ORIGIN      
        1 acacattttt tagctgaagc tgtaaatttc atgtcttaat tttttttagt gagcttttat
       61 actggatttg taagaaaaaa gaaggagagg atcacatatg tcggattggt tggatattgc
      121 aggaaaagta gtaattgtaa ctggaggttc ctcaggaatt ggaagaagta tcgtcgaaaa
      181 tttgttgaag cagaatgcac aagtagcgaa ttttgatgta actgaatgcc gtatacagca
      241 tgaaaaccta ctatctttaa aagtcgatgt gtcttctaaa acggacatag aagaagggat
      301 atataaagta atgaaacatt tcaagacgat cgatggtcta gtcaataatg caggaataaa
      361 tattccttct cttctaatag atagaaatca tccaaaaagc aagtatgaat tatcagaaca
      421 agtttttgat aagatgatcg ctgtcaatca aaaaagtgta tatttgatga gccaagcagt
      481 tggaagaatc ttggtgcaaa agggatcagg agtgatcgtg aatctttctt ctgaatcagg
      541 attggaagga tcagaaggac agagctgtta tgctgctaca aaagcagcga tgaacagttt
      601 tactcgttct tgggcaaaag agttaggaaa gaggaatgtt cgtgtggtag gtgttgcacc
      661 agggatactg gaagaaaccg gtttgagaac acaagaatat gaagaagctc tttcttatac
      721 gagaggtata agcgtcgaac agctgagaaa cggttacagt cgaacaagta cgatccctct
      781 aggaaggagt ggtaagctgc aagaagtggc ggatctagtc tgctattatc tttcagaaag
      841 atcaagttat atcactggag tcacgactaa tatttcagga ggtaaaacaa gaggataaag
      901 gatagggggg acgcacgtga cgctagtaaa cagatggtac caattattga aattactagt
      961 cactcacaag aaagtttcaa tccaggaagt gcaaaaagaa atcaaaacca gtaatcaaac
     1021 cataaaaaaa gatattgggg agttgaatga agaaattgcc ggtattgcaa aaatcatcca
     1081 aaaaaacaaa cattttgaac ttgaaattta tgattttgat ttttttgatg agatcatgca
     1141 aggaaaattg aaaacagcca gtgatttcaa ttcggcatcc aaaagaatcg cttatttact
     1201 aaataggctg atcgaaacag aggatttctt acgtatggat gatttgtctg aagaagtcgg
     1261 tgtcagcaga ggaacagtgg tcaaggatct aaagacatta aaagagatga tcaatgattt
     1321 tgatgtaaag attacaggca caccaaataa aggactaaga ataataggag atgagctaga
     1381 attacgacta cttttatttt attttgtttc tccctacttt tcagagacga aaacagaaca
     1441 gtttctagaa gaaaaacaag caatcaaaca ttttcagaag aagacgaatg ctagtaaaca
     1501 agaaatcacc cttttaacga aagttatcgg tatttctttg aatagaatct ctagtaacta
     1561 tctgttgaag aagcccacca atcgttacag tgggtgtttt gaatatatcc aagaattcaa
     1621 tgaactgatc aatcagttgg aagaaatcta tgaactaact ttgagccaat atgaaaaaga
     1681 ttttatcagt tttcctttga acatcatcaa cacaagtata acgatgataa ggaaagctaa
     1741 ctctgatttg cgcttatatt tcgaccccat gatgatgcgt attcgacgat tagatctcat
     1801 tgatctggat gagtccttct tatatcaaga gatgaaacag cacctctctt tattgataaa
     1861 tcgggtaatt tttcgttatc gattgactga tttattttat ggtgagatag aaaaaaagta
     1921 tcctttttct taccaaatcg ctagcgaatg catacaagca ttagaagcta tcttgcaaca
     1981 taagatctct aaagtagaga tcagctacct aacgttgtat ttggagttag catttcgata
     2041 tcaaaaagag catttttcct ctaaaaaagt agccattgtt catacgacag gaaaaggaac
     2101 agcattaatg atccaaagac aaatcaaaag gatattgggg agggatatac aaataacaca
     2161 tgtttcagaa gaacaatatg aaaaagtaga tctaaacaaa tatttcgctg tttttacaac
     2221 gattccgtta aaaaatgtaa atgaagagac accggtgatc catttgacta atctttttga
     2281 tgactcttgg ttaagggcac aatggcaaaa agcaaaaaaa aagcgattag ccgggtttca
     2341 ttctactgtt tttttctatc aaatatggaa agaaaaaaag acgtatcaag aatatttaaa
     2401 caaattagtc gctcctttga tcagaggcta ttatgtggat gaagaatttg aaaaaaggat
     2461 cttgaaaaga gaatatcagc aaagcacatt tgttaacaaa gaatttgctt ttcctcacac
     2521 aaccaaccag ctatctcaac acattgtttt atcagtaggg ctaataactg atcaaggttt
     2581 agcagaaaca ggaggaaatg gagttatttt tttatttgcc atacctgaag aaatgacggc
     2641 tgaagttgag aatgaattat ttgagctata cgatatccta tttgaactca taaaaatggg
     2701 aatagatgag tttattgaat tagcaccgac acaagaatca tttttaaaac taatcaaaag
     2761 taaaggagag agacttcatg aatgatatgt atctattcat agttttcgca gcttcagctt
     2821 atgtgctgca agcagttatc agttggaagc aactaaagca tttcaatcaa acatatagcg
     2881 aactgcgaag aaaaggaaaa gtagcgattg gtaaacgacc tgggaaaatc cgttcaggca
     2941 cgattgttat gctagctata gacgatcatg atcaagtttt agaagtcaga aaaatgcaag
     3001 gggttacatc tttggcaaaa tttcattcat tacctgtcta tgcgggtgaa gatatccatt
     3061 acatcgatcg ttaccatccc tcagttcgag cggaagatcg tttgacacaa gcagctttag
     3121 aaaatgccag agaagtatac ttacgaatag aggcaggaaa tgaaaaaagt gaacaacctt
     3181 cattgccttt tattgctatg ctcaataatt ttcggatatg gaaaagtacg atcaaagaaa
     3241 aaaggagtga caaacgatgg atatcataac aaaatttgct gagggattta tgaggctgtt
     3301 ccaaacaggt gcagaaacat tcataagctg gatgacagga atcgtaccag ttgtcttgat
     3361 gctgatggtt gcaatgaata cgctgattgc ttttttaggt gaagaccgag taacgaaagt
     3421 agccaaatta tcagcgaaaa atccgcttac tcgttacctt gtgttaccgt tcttatctgc
     3481 ttttatgttg ggaaatccta tgtcatttac aatggctcgt ttccttccag aatactacaa
     3541 accaagctat tatgctgctc aagctcaatt ttgtcatacg agcaatggtg tattccccca
     3601 tattaatcct ggagaattgt ttgtatggat ggggattgct caagggatcg aaaccttagg
     3661 gctaaattcg atggagctgg ctatccgata cttgcttgtg ggactgctga tgaactttat
     3721 tggtggttgg ataactgatt ttactactgg atttgtatgc agacagcaag gaatcgttct
     3781 tagcaaaaaa gtagagcttt cagttgatta gagaaaggag agttttaaat gtatggtgat
     3841 attaagatcg taagaggaaa cggcgggtat ggaggtcctt tgattattag gccgacaaaa
     3901 gataaacaca agttcatcta tgtcacaggt ggaggtgaga ggccaatgat cgtagataaa
     3961 atagaagaac tgacaggtat ggagtctatc aatgggttca aaacatcaat acctgatagt
     4021 gaaatcgcgc ttgcaatcgt tgactgtgga gggactctta gatgtggaat ttatcctaaa
     4081 aaagggatac caaccatcaa tatcgtttcc acaggaaaaa gtggcccatt agcacaatat
     4141 atcactgaag atatttatgt ttcagcagta gggttagaac aaatacagcc actaatggaa
     4201 actgatgata acaaagaaac agtaaatact gcatctccag ataaaaagta caaatatgat
     4261 acttctaaaa agataaccga gcaaaaagca gagcagcaaa actggatagc aaaaattgga
     4321 atgggcgcgg gaaaagtagt tgctacattc aatcaatcag caagagaagc agtagatacg
     4381 atgatccgga cagtgatccc ttttatggct ttcgtttccc ttttgattgg tgtgatacaa
     4441 ggttctggga tcggtaatgt atttgctaac attatggcac ctttagcagg aaatgcagtt
     4501 ggattaacaa tcattggctt tatttgttca ctgccatttt tatctccttt gcttggtcca
     4561 ggggcagtta ttagccaagt gattggaaca ttgatcggtg tagagattgg gaaaggaaat
     4621 attcctcccc aattagcatt accggcacta tttgctatca atactcaaaa tgcatgtgat
     4681 tttattccag tcggtctagg tttggaagaa gcaaaagctg agacagtaga agtaggtgta
     4741 ccatcagttc tttattcaag atttcttaac ggcatcccac gagttatcat tgcttgggta
     4801 gcaagttttg gattatatag ctg
//
LOCUS       DAIGOK010000145         4809 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.147, whole genome shotgun sequence.
ACCESSION   DAIGOK010000145 DAIGOK010000000
VERSION     DAIGOK010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.147"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..850
                     /locus_tag="NOA01_002399"
     CDS             <1..850
                     /locus_tag="NOA01_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCI0633561.1"
                     /translation="PKRKMVINKKAIAPLIGTSLLVGPMMVTAVPQHVHADEQGSQTV
                     ADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLASAPNYNLFGVKGSY
                     NGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTSFSTGDYHYAGVWK
                     SNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTGSATTTTSTTTTTT
                     TTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMAKNGMSMSSYSLLV
                     GQELNV"
     gene            990..1916
                     /gene="cysK"
                     /locus_tag="NOA01_002400"
     CDS             990..1916
                     /gene="cysK"
                     /locus_tag="NOA01_002400"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCI0633562.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2152..2598
                     /locus_tag="NOA01_002401"
     CDS             2152..2598
                     /locus_tag="NOA01_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCI0633563.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2871..4223
                     /locus_tag="NOA01_002402"
     CDS             2871..4223
                     /locus_tag="NOA01_002402"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HCI0633564.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4394..4681
                     /locus_tag="NOA01_002403"
     CDS             4394..4681
                     /locus_tag="NOA01_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633565.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1653 a          800 c         1006 g         1350 t
ORIGIN      
        1 acctaaaaga aaaatggtga tcaataaaaa agcgattgct cctttaattg gaacgagtct
       61 tttagttggg ccgatgatgg ttacagcggt accgcaacat gttcatgcag atgaacaagg
      121 atctcaaaca gtcgctgatc ctcaagcgtt tatcgatcaa atcggctggt ctgcgtcaga
      181 agttgctgca agtaatgact tgtatgcttc agtcatgatc gctcaagcgt tgttggaaag
      241 tgcatatggc acttccggat tagctagtgc gccaaattat aatctattcg gtgtgaaagg
      301 aagctataac ggacaggtcg tttatatggc aaccaaagaa tatgtaaacg gacaatgggt
      361 aacgatgaat gagccattcc gaaaatattc ttcttattgg gaatccttcc aagaccatgc
      421 gaatgttctt cgttcaacca gtttttccac gggcgattat cattatgctg gcgtgtggaa
      481 aagcaataca acaagttttt atgatgcaac agcgtacttg acaggtcgtt atgctaccga
      541 tccaaattat ggggccaaat tgaactcatt gattaatact tataatttaa cccgttttga
      601 tacgccatca acaggttcgg ctacaacaac cacaagtaca acaacaacga ctactacgac
      661 gacttcaacg tcatcatctt caacaacaga tgtaaataca aataatggtc agacatatac
      721 agtcgtgtca ggtgatacgc tatgggatat tgcacaaaaa tttggcatgt ctgttgatga
      781 tctaatggcg aaaaatggca tgtctatgtc cagttattct ttattagttg gtcaagaact
      841 aaacgtttga tttagataaa aaggcaaaga aattaccata tatggtatat ctttgtcttt
      901 tttttgctag tcaaagacga atcactcgcc ataaatgaac agattgtatt atgataagta
      961 agaagagcaa agacaaggag gaattgaaga tgacagctat ctataactca gtcaccgaat
     1021 tgattggcaa aacgccaatc gtcaaattaa ataaaatcgt accagaagat tcagcagatg
     1081 tttttgtaaa gcttgagttt tttaatccag gaggaagtgt aaaagatcgt attgctttaa
     1141 gtatgataga aaaagcagaa cacgatggtc tactgaaacc aggtgatact atcattgaac
     1201 ctacttctgg aaatacagga atcggattgt cgatggtagg agttgcaaaa ggatataaag
     1261 taatcatcgt gatgcctgag acgatgagta tcgaaagacg tttattgatg aaagggtacg
     1321 gcgcagagtt gattttgact cctggtgcgg atggtatttc tggatcaatc agagaagcag
     1381 aacgtttggc aaaagaaaac ggctactttt taccattaca atttgaaaat gaagcaaatc
     1441 ctcttgtgca tgagaagacg actggtcctg agatacatca agcatttgga gtaaatggct
     1501 tggacgcgtt tgttgcaggt atcggtacag gaggaacgat cactggcgct ggccgagaat
     1561 taaaacgtgt gtatccgaaa atcgaattaa tcggggtaga accagcagaa tctgctattt
     1621 tagaagggaa agaagcggga cctcacaaaa tccaaggaat cggtacaggt tttgttccta
     1681 aaacactgga tacttctgtg tatgataaag tcctttctat tagtggagac gaagcgatgg
     1741 aaacagctag agaagttggg cgaaaagaag ggattttagt aggcatctca tctggcgctg
     1801 caattgctgc cgcgctaaaa gtcgcaaaag aattaggaaa aggcaagaag gtattagctg
     1861 tggttcccga caatggtgaa cgttatttat ctacagccct ttaccaagag atataaggca
     1921 cttggttttt ctccagatag gtttatgtaa aaaactttta aagatgaaaa gaacacacaa
     1981 aaatttatga tgagaaaaat tatttgaata gagacgtaga atagtgaacg cgactgtttt
     2041 acgtcttttt tcattaaaat acaataaaat tgaattttct tttcatgtta agtggatttt
     2101 aaaatgtaaa aatagtatga taagacgaga ataacaacgt ggggtgttta catggaaaat
     2161 aagttagtcg aagaggttat cgaaaagtta aaaaaagaga acattcgaat cacacctcaa
     2221 cggtacgcgg ttttagaata tttgattgaa catcattccc atccaacggc tgatgagatt
     2281 tataaagcac ttgaacatcg gtttccaaat atgagtgttg cgactgttta caataacctt
     2341 cgcttattta cttcgattgg atttgttcaa gaaatgaagt acggcgatgc ttctagccga
     2401 tttgatttca gttcaaagcg tcattatcat gtaatttgtg aaaactgcgg taagattgtt
     2461 gattttcatt acccagggct tgaagatata gaaatggctg caagcagact gactgatttt
     2521 gagattaatg aacatcggtt ggaattgtat ggactctgtc cggattgtaa agagtcagca
     2581 aaaaaggcgc aaatgtaaaa acagtttcta caagtgttat acctaaaaaa agctagctgt
     2641 gtaaaaaaaa cgctagcttt tttatttttt tgtcttttat tcacaatctt agatataaaa
     2701 gtgaatatta ttaaaataag aatcttgtag atattgggtt cttatgcttg aaaaggaatg
     2761 aaactacaaa caaacctttg tgaattcaca agcattaaac tgttgtatcc gttttatttc
     2821 tgttatagta gttttgtcga aggacaaaat gttaatcgga gggaccataa atgaaagtag
     2881 tagtaattgg ctgtactcat gcaggtactg cagctgtaaa aagtatttta aaaaatcatc
     2941 cagaagcaga agtgacagta tatgaaagaa atgacaatat ttctttctta tcttgcggta
     3001 tcgcacttta tgtcggcgga gtagtaaagg acccagcagg cttgttctac tcaaatcctg
     3061 aagaattatc ttcattagga gcaacagtta aaatggaaca cgatgtaaca gacgtcgata
     3121 cagaaaataa aacagtaacg gcaaaagatc tgaaaacagg agaaacagaa acggtttctt
     3181 atgataaatt agtgatgaca actggttcat ggcctattat cccaccgatt agtggaatcg
     3241 atgcgaaaaa tgtactccta tgcaaaaact acaatcaagc aaatgaaatt attgcacaag
     3301 cgaaagaagc aaaacgtgta gtaattgttg gtggtggata tatcggtatt gaattagtag
     3361 aagcttttgt cgaatctgga aaacaagtga cattgattga tggattagat cgcatcttga
     3421 ataaatatct agataaacca ttcacagata tcttagaaaa agaattgaca gaccgcggtg
     3481 ttactcttgc gctaggcgaa aatgttactg aatttatcac agatgaagaa ggaaaagtga
     3541 aacaagtagc tacaccaaca gatacatttg atacagatat ggtcatttta tgtgtgggct
     3601 tccgtccaaa tacaaaactg atcgaaggaa aagtagatac actgccaaac ggggcaatca
     3661 aagtgaatga atacatgcag acaagtaatc cagacatttt tgcagctgga gactctgcag
     3721 tagtcaacta caatccaagt ggcacgcaaa actatattcc tttggcaaca aatgccgttc
     3781 gtcaaggttt gcttgtggga aataacttga cagatcataa aatggcgtat cgtggtactc
     3841 aaggaacttc aggactttac ctatttggct ggacgatcgg ttcgacaggt gtgaccaaag
     3901 aaagcgcaac tttgaacgat ctagaagtcc aagcgactgt atttgaagat aattatcgtc
     3961 cagagttcat gccaactact gaaaaagtaa tgatggagct agtctatgaa aaaggaacga
     4021 atcgtatcgt aggtgctcag ttcatgtcca aatacgacat tacacaatca gcaaatacca
     4081 tgtctttagc tgttcaaaat aaaatgactg tagaagatct cgcactttca gatttcttct
     4141 tccaaccaca ttttgaccgc ccatggaatt acttgaacct acttgcacaa gcagcgctag
     4201 gcgaaatcga aactgcacaa taaagaaaaa gggttgtgac acaattttgt cacgaccctc
     4261 tgtctttttt tgtgaaaagt acaattagtt gtctttcttc gaattttttg tcacaataag
     4321 cagcaaagat aaagctgtta cataaaagaa caagcagctt tatttgattt tgaaaagaaa
     4381 ggtgagaaag aagatgaata aagaatattt tgtccaacaa gatgcatttt tagaagatta
     4441 taaaagtgct tcgaagaaat taacgatcaa tattgatgag ttggcagatc aagtactctc
     4501 tcagttgaat gaaacgcaag aggaatatta cacgatgcca gcaaatgacg ccaaagataa
     4561 tgtttctcat cgttttcctt tcagtagacg catctactca gatgaaaatg acggcacatt
     4621 gaatacagag tacacatatg aaggtcagcc atatgtgcta gaaactgatg atgatcttta
     4681 aagacagttt ttgaaaaggc cgctcatcct taagtgatga caaaaaatca tcacaattac
     4741 cgatgactgt ctctttacta tcttttaatc agaaaaaggg gttatgacaa gctttgtcaa
     4801 ccccttttt
//
LOCUS       DAIGOK010000146         4804 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.148, whole genome shotgun sequence.
ACCESSION   DAIGOK010000146 DAIGOK010000000
VERSION     DAIGOK010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.148"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            6..863
                     /locus_tag="NOA01_002404"
     CDS             6..863
                     /locus_tag="NOA01_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCI0633566.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQRRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            885..1394
                     /locus_tag="NOA01_002405"
     CDS             885..1394
                     /locus_tag="NOA01_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCI0633567.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            1431..1832
                     /locus_tag="NOA01_002406"
     CDS             1431..1832
                     /locus_tag="NOA01_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633568.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            1944..2297
                     /locus_tag="NOA01_002407"
     CDS             1944..2297
                     /locus_tag="NOA01_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633569.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            2331..2774
                     /locus_tag="NOA01_002408"
     CDS             2331..2774
                     /locus_tag="NOA01_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCI0633570.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            2791..3876
                     /gene="uxuA"
                     /locus_tag="NOA01_002409"
     CDS             2791..3876
                     /gene="uxuA"
                     /locus_tag="NOA01_002409"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCI0633571.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            3869..4582
                     /locus_tag="NOA01_002410"
     CDS             3869..4582
                     /locus_tag="NOA01_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HCI0633572.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         1659 a          734 c         1084 g         1327 t
ORIGIN      
        1 gggaaatggc tgaaataatg gataatcaaa caggtgctcc tgaagaaatc acgaaaaaag
       61 atgtaacaaa agcctatctg agatggcatt ttgcaaacga aattcctcac tcttttgaac
      121 gttacttggc gccatcacta ctatacggaa tgatgccaat cttgaaaaaa ctttataaag
      181 atgaagactc tttaaaagca gcatataaac ggcagttact gttcttcaac actcaattaa
      241 gctggggtgg cggtgtgatt actggcctaa tgtcttctat ggaacagcga agagcagaag
      301 aagagtatga agaaaaagag atcttgatgc aggatgactt gatgtataac acgaaagcgg
      361 gattgatggg ggcactagca ggaatcggag atgcaatcga ttcaggaacc gtccaatata
      421 tttttatcgc aatcgcggta ccttgggcac aacagggaag tcctttaggc gcactttttc
      481 cttttatctg ctttgggttg tatcaggttt tactaggggt atttttcgca agacaagctt
      541 tcactatggg ccgtaatgct acaggcctaa tgcaaaacac tggtgttcag acagcgattg
      601 aaatgctatc agttttaggg ttgtttatga tgggggtgct tgcgggaaac tatgtaaaag
      661 tagaatcttc attgaaattt gcgatatccg gacgtgagtt tgttatccag gaaattcttg
      721 atcaaatcgt gccagggata ttacctttag ctgtcgtaat gggtgtgtac ttgttctata
      781 cgaaaaaagg attgaaagtc acacaagcat tattatggct gactggaata ttaattgtgc
      841 tagcaggaat cggtatttta taaaaagaag ggaacgtgaa taagatgggt gtagtcaatt
      901 tagcgcgtgt ggatgagcgt ttgatccatg ggcaagtaat ggtaacgtta tctcaaaaaa
      961 gtggagtgaa ttccattttt gttgtagacg aagttgtagc aaaagataaa tttatgagag
     1021 atctttacaa aaatgcagga agccgtacag gacaaaaaac aattgtcatt acaccagaga
     1081 aagctaaatt ctattgggat gaatatcaat ttaaagaata caattgtatt ttgatcgcaa
     1141 aaacagtcca aacgatttac gacttagtaa aacacggaat ccccatggaa gaattgaata
     1201 ttggagggat tgctcaaaaa aatcctgata cagatctttt agtgacgaaa tctgtttatt
     1261 taaataaatc agatgctgaa aaattgaaag aaatgaatga acaatatggc gtaaagaata
     1321 tttattttca agccactcca tctgcaccta aaacaggatt aaaagatgta ttgaacaagt
     1381 ttgatttgtc ataaacaaat gcttagaaaa aagataaaga gggcgaaaaa atgaagaaga
     1441 acgagctgtt tcgtgattgg gaatttcgtt atcgctatat ttaccgtaag cgccgtacaa
     1501 aaaagagtaa acagcgtttt ctttcagcat tagtatcaga tatttacagt atgagaacag
     1561 atgtgacagt cattgcttac gatacgctag cctaccgatc aaaaaatatt tatgttggag
     1621 atatcgaaaa agcagagaaa gttatctgta cttattacga tacacccgtt catgcattag
     1681 gaagttattt catgtttgat tggaaagacc aaaggaaaaa aacaatctac tctatattat
     1741 tgtccttcat tttgttgttc agtttaggtt ggtggggaat gatgatatat aacaaaaatc
     1801 ctcatcacgt attcgatctg ctatccgtat aaacaggtat cactgtacta gcatttggga
     1861 gctacttttt cttattggga aaagcagctc gcggatggcc tagcagacaa acttttattc
     1921 gaaatacttc atccatatta acgatgctag aaatgatccg tacgattgat gatccgagtg
     1981 tagcgtatgc gtttgttgat gaaggctgtt atggagaaaa gggcttagac agcgtgagat
     2041 cgggtatgaa aaaagaggct attctttttt atctagactc tgttggggca gatacaccgc
     2101 tacagttttc gggaaattat ttttcaaata aagaacagtg gctgaaacaa gttgataagt
     2161 tgaaagaaaa aaacgtcaat tatattttta gtgcaagaaa aaagcaagcg cagttcttct
     2221 atttgaccaa aacagattta cgagggaaaa catttaattg gcaaaatgcc aatcagatta
     2281 ttgctctttt tcgataagta aaaagtgcaa aacaaggagt gagaagacta atgttggaaa
     2341 tcatgatcgt tacacatggt aagttgagcg aaggactgaa agatgcggcg gaagtaattg
     2401 tcgggacatc gagccatatt gaaacaatgt gcttagagtc tggggaggac atccaaacat
     2461 tcggagagaa ggtcaaagaa aaattaacta tgatcaacac agcagaaccc atgctagtat
     2521 ttgttgatct aattagcgca agtccgtaca atcaagcgct cttggcagtc aatgaactga
     2581 ctgaagaaaa gaaagcagcc acttatgtca tagggggcgt gaatctgcca atgttgttag
     2641 aagcaatcaa tcatcagatg cttcacacac cgatagaaga agcggtggaa caaattgtca
     2701 aacaaggaaa agataattta actgtttggc atgcttcagc ggaagaaaaa aaattagatg
     2761 aggacgattt ttagaaggaa ggctgaaaag atgaagtggg gatttagatg gtatggtgaa
     2821 aaaggggatt cgatcccatt aaaatatgtt cgccaaatac cgggaataaa tgggataatt
     2881 ggaactttgc tgactaaaat gcctggagat gtttgggaga tttcagaaat tgaggagttg
     2941 aaagcatctg ttgagagcca aggactggaa ctgcttggaa ttgaaagtgt agcgatccac
     3001 gatgcaatca aggcagggac agatgaacga gattactata ttgataacta tatacgaaca
     3061 atcaaaaact tgtctcgatg tggggttcga ttgatttgtt atagcttcaa accaattttt
     3121 ggatgggcga aaactgattt gtttaatgaa aacaacgatg gaagctattc tcttttatac
     3181 gatcagaaaa tcgtcgatca gatggagcca ggagaaatgt atcaattgat acacagccag
     3241 tcaaagggat tcaaattgcc gggttgggaa gaagaacggt tgaaaaaatt cgctcagctg
     3301 gaaaaactgt atgaaggaat gaccgaagaa gaattatttg aaaatctgac ttatttttta
     3361 gaaaaagtga ttccagtgtg cgaaaaagca gatgtaaaaa tggctatcca tccagatgat
     3421 ccgccatggg aactttttgg ttttcctaga gtgacgaaaa atcttggcga cttgaagaaa
     3481 attctaaatg cagtagattc tccatataat ggactaacgc tgtgcacagg atctttaggg
     3541 gcaaatccgg aaaacgattt agttgaaatc atccatgagg taggttcgcg catccatttt
     3601 gtccacttca gaaacgtgga gtttttagga gaaagaaagt tcaaagaaac ggcacatccc
     3661 tctacagaag gctcccttga tatgtatcat attatgaaag cgttggtgca agaagggttc
     3721 gacggtccta tacgccctga tcatggtaga accgtgtggg gtgaagtggc tatgcctgga
     3781 tatggcctgt atgatcgtgc aatgggactc acttacatgc aaggtctata tgaggcgata
     3841 agtaaggaaa aaccactggg agagcattat gaataaaatc aagctcttca tctttgatat
     3901 ggatggcctt ttattagaaa ctggacgttt agcttatcgt gcttatgtaa aatcagcaca
     3961 aaagtatgat tatgaaatgc gtaaggaagt ctattattta ctaactggcc aaacagaaat
     4021 ggcgataaga gaacaaatgg gcattttgta cggagaagat gttccatata tcaaatggcg
     4081 agaagcaatc aatcaatata aagaaaaaat cgtgaaagaa gataagagag tatatacgaa
     4141 aaaaggagca gaagaaattt tatcttttgc aaaagaaaga ggaatccata caatcgtggc
     4201 ttcgtctaat acacgggtaa aagtagaaat gtatttaaaa atggaaaatc tatatgcctt
     4261 gtttgatcaa atcatttcag gggatgatgt aaaaaaagga aagccggaac cagagatctt
     4321 tttgaaagct tgttcaaaaa tgaatattcc tccaagtgaa gcacttgtat ttgaagactc
     4381 gattgcaggg atcgaagcag caaggcgagc gggcattttg tcttttctca tcaaagatca
     4441 tttgataggg ttgcccgatc ataatgggaa gcataagttg aaagtagatg taagtttata
     4501 tgaatccaat aaagcagact atgaatttta tgacctgcat caagcaaaac gatttttaga
     4561 aagtaaaggg ttgtatctct aataaagagg actattttct agtcctcttt atttattgtc
     4621 ctgcacgact gagggaacag atgaaaaaga agtaattttt tattttgaag tatctacctt
     4681 atttgtagtg aacaatttag aacaaacaca aaacgcatct actgttatct accagtagat
     4741 gcgtttaaag agaagaacga gaatcgtcca tatgagggtt tccactcctt atgcagattt
     4801 tttt
//
LOCUS       DAIGOK010000147         4800 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.149, whole genome shotgun sequence.
ACCESSION   DAIGOK010000147 DAIGOK010000000
VERSION     DAIGOK010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.149"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            37..588
                     /locus_tag="NOA01_002411"
     CDS             37..588
                     /locus_tag="NOA01_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HCI0633573.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
     gene            1264..1518
                     /locus_tag="NOA01_002412"
                     /pseudo
     CDS             1264..1518
                     /locus_tag="NOA01_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1588..2100
                     /locus_tag="NOA01_002413"
     CDS             1588..2100
                     /locus_tag="NOA01_002413"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCI0633574.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLC
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            2097..3134
                     /locus_tag="NOA01_002414"
     CDS             2097..3134
                     /locus_tag="NOA01_002414"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCI0633575.1"
                     /translation="MISFSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
     gene            3294..3470
                     /locus_tag="NOA01_002415"
                     /pseudo
     CDS             3294..3470
                     /locus_tag="NOA01_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            3525..4800
                     /locus_tag="NOA01_002416"
                     /note="aph(2'')-Ia"
                     /pseudo
     CDS             3525..4800
                     /locus_tag="NOA01_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
BASE COUNT         1826 a          702 c          916 g         1356 t
ORIGIN      
        1 agcgagcaag acaggaacga gagaacaaac atactcatga aaagactaga ggcttcgacc
       61 tcgaacttta ataataagtt agatgttttt gaagaaaaga ctaagcatgc aagattaaac
      121 tttgaaacaa ctgcaaagca ctatattaaa cgacttgatg aagataattt gaaattagat
      181 tttcaacaag ctattcaaga tgaattaagc gatactaaag acgaaatacg tgaagtgacg
      241 aaacaagcga gagaagaaac aaaagagtat aaagaaatct tagaaagtaa aataaaagac
      301 cataacaaag tggttgataa atcgaataca gcgctaaaag tgatgacaaa aggcgttaca
      361 aatatatttt ttgtgctaat tatttttgta cttgtaatgc ttgtgacagg tccgataggt
      421 catttctttg gtattgaaca tttatatagc tttattaatg gttttattga tgaccacgaa
      481 agtgcatggc gatatttgat gttgatactt tatgcagtac cctatgtatt cttcgctatc
      541 attctgtggc tcatagcatt gttttttaga gtatttaatg acctttaggt agatttatat
      601 ataaaaaggg ctaaatacgc aaaatacccc cttaaaatgc aaaataaatg caaataaggg
      661 ggtaactcct tatgaaaaaa caactaatag tattataaca cgtttttatg ccgagaaaac
      721 ttattggttg gaatgggcta tgtgttagct aacttgttag cgagttggtt ggacttgaat
      781 tgggattaat cccaagaaag taccaactca acaacacata aagccctgta ggttccgacc
      841 aataaggaaa ttggaataaa gcaacgaaag gagttgagta aaatgagtga gaaaacgaag
      901 aaaatcattc gagtatcatt agctttttca caagtagtag tgcaatcatt gatagtttat
      961 tatacttacc agcaatataa attatctaaa gagaattaag ttttgattat gtaataagtt
     1021 ttggtactaa aaaagacttg atctaattag accaagtctt ttgttagtgt tatattaata
     1081 acaaaataaa aataagaagt cgctcactcc ccgaccaaag tttgtgagca aaataaacag
     1141 tcgatgtttg tttcttaatt gtatatcttg atattaaacg atttaagatt attcttcaag
     1201 atatatattc gcaaatgtaa ttggaaaagc gacttcttaa acaaaattaa ggagtcgctt
     1261 tttatggcaa aaataacgaa aaatgagcaa gaaaatttgg tggaaaatta cacaaaaaaa
     1321 ccggcgtact ctaatagccg gttagacgca catttcgtgt gcacttctaa tccgaaattg
     1381 agctttgacg ctatgacgat agtcggaaac cttaataaaa acagcgctaa aaagctatcc
     1441 gattttatga gtttagatcc acaaattaga ctttgggata tacttcaaac aaaatttaaa
     1501 gctaaagcac tacaagaccc cctatgttag gctagttgtc gtataggata ataagttata
     1561 ataaagacta tacggaggat gattaccatg acaaataaaa acaatgaacg ttactctacc
     1621 gaactgaaag cgacagccat caagaaaatg atgccacccg aaaatagggg gattcgccaa
     1681 ttaagtcaaa ctttgggcat tccagaagcg actctttgtt cttggcgtaa aaaagctaga
     1741 attcaaggga atactacacc cggaacgaaa aaaaatgtcg aaaaatggtc aagtgaagat
     1801 aaatttttaa ttgtgatgga atcattctca atgaaccaag tagaacttgc ggaatattgc
     1861 cgaaagaagg gcttatttaa agaacaaatt gaggcttgga agaaaacgtg cttatctgcg
     1921 aatgaacagg aagaaaatcg aacgagagaa cttgcaactg aactaaaaga agagaaaaaa
     1981 caagcgcgtc aattagagaa agaattacgt cataaggaaa aagcattagc ggaagccgct
     2041 gctctcttat tactaagaaa aaaagcacac gcgatttggg gggaccaaga ggaagaatga
     2101 tttcattttc agatcgcaaa caagctgttg aacttattca agaagccaat cgcaatgggg
     2161 cccgtctcac ctatgcctgt aatgagctga atattagcgt tcgaacctac gaacgatgga
     2221 cgcgtgaagg tacgatagcg catgatcaac gccctttggc gaaacgtcct gtacccaaaa
     2281 ataaactgac ggatcaagaa cgcgaaaaaa taatagaaac cgtttcaaaa aaagaattta
     2341 tgaatttgcc acctagccaa attgtaccaa aacttgcgga ttgctcgatt tatattgcat
     2401 cagagtctag cttctatcgt gtattgcgag aaaaaaatat gcaacatcac cgtggccgaa
     2461 gtcaagtttc acaaaaacgc ataccgccaa gtcaccttgc gacgaagcca aatgaagttt
     2521 ggacttggga tattacttgg ctaaaaggtc caataaaagg actcttttac cggttatatc
     2581 tcatcattga tttatttagc cgtaaaatgg taggctggga gatttgggaa tcagaagaag
     2641 ctcgtcatgc cgaaaaactt attaaacaga cggtgcttaa ggaacgtatc caaggacagc
     2701 cgttggtttt acactccgat aatggcagcc ctatgaaagc cgcgactttt caagtcttac
     2761 tcgaaaaatt aggaattcaa agttcttatt caagaccacg agtgagtaat gacaatcctt
     2821 attcggaagc ggctttccga acgttaaaat atcgaccaga atatcccaat aacggcttta
     2881 aaacaataga tgatgctcga aaatggacga aagagtttgt cgattggtat aacgattctc
     2941 atttgcatag tgggattcaa tttgtgtctc ctgcagcgtg tcatgaagga cgacatctcg
     3001 ctattttaga gaaacgaaag caggtttacg aggacgctaa aaagcgccat cctgaacgct
     3061 ggtcaaaagg tgttcgaaat tggactccac atcaatcggt ggcgttgaat cctttaaaag
     3121 aaacagaaag ttaacaggag cataatcttt caagattatc aaataaccct cgtcacccaa
     3181 taggggaaaa agttattctt cccctattgg ataacgaggc caaccgacca tagggaggtt
     3241 ctgctttttg ataggtggaa aataaaaata cgacaactat attgacaaac accgaccatc
     3301 gtccagtaaa gagacgcaat aaattctatc gaagtttacg cactgcctct acaacgatta
     3361 aaggcatgga agccatccga ggattatata agaaaacccg aaaagaaggc actctcttcg
     3421 ggttttcggt ctgtactgaa atcaaggtat tattgggaat cccagcttaa atcatagata
     3481 ccgtaaggga ttttattctt tatttaaaac tttgcaacag aaccgaatca ttaaaaaaac
     3541 attatacaga gccttgggaa gatgaagttt ttagagtaat tattgaatat aacaatgttc
     3601 ctattggata tggacaaata tataaaatgt atgatgagtt atatactgat tatcattatc
     3661 caaaaactga tgagatagtc tatggtatgg atcaatttat aggagagcca aattattgga
     3721 gtaaaggaat tggtacaaga tatattaaat tgatttttga atttttgaaa aaagaaagaa
     3781 atgctaatgc agttatttta gaccctcata aaaataatcc aagagcaata agggcatacc
     3841 aaaaatctgg ttttagaatt attgaagatt tgccagaaca tgaattacac gagggcaaaa
     3901 aagaagattg ttatttaatg gaatatagat atgatgataa tgccacaaat gttaaggcaa
     3961 tgaaatattt aattgagcat tactttgata atttcaaagt agatagtatt gaaataatcg
     4021 gtagtggtta tgatagtgtg gcatatttag ttaataatga atacattttt aaaacaaaat
     4081 ttagtactaa taagaaaaaa ggttatgcaa aagaaaaagc aatatataat tttttaaata
     4141 caaatttaga aactaatgta aaaattccta atattgaata ttcgtatatt agtgatgaat
     4201 tatctatact aggttataaa gaaattaaag gaactttttt aacaccagaa atttattcta
     4261 ctatgtcaga agaagaacaa aatttgttaa aacgagatat tgccagtttt ttaagacaaa
     4321 tgcacggttt agattataca gatattagtg aatgtactat tgataataaa caaaatgtat
     4381 tagaagagta tatattgttg cgtgaaacta tttataatga tttaactgat atagaaaaag
     4441 attatataga aagttttatg gaaagactaa atgcaacaac agtttttgag ggtaaaaagt
     4501 gtttatgcca taatgatttt agttgtaatc atctattgtt agatggcaat aatagattaa
     4561 ctggaataat tgattttgga gattctggaa ttatagatga atattgtgat tttatatact
     4621 tacttgaaga tagtgaagaa gaaataggaa caaattttgg agaagatata ttaagaatgt
     4681 atggaaatat agatattgag aaagcaaaag aatatcaaga tatagttgaa gaatattatc
     4741 ctattgaaac tattgtttat ggaattaaaa atattaaaca ggaatttatc gaaaatggta
//
LOCUS       DAIGOK010000148         4788 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.150, whole genome shotgun sequence.
ACCESSION   DAIGOK010000148 DAIGOK010000000
VERSION     DAIGOK010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.150"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            564..989
                     /locus_tag="NOA01_002417"
     CDS             564..989
                     /locus_tag="NOA01_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633576.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            1019..1183
                     /locus_tag="NOA01_002418"
     CDS             1019..1183
                     /locus_tag="NOA01_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633577.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            2141..3118
                     /locus_tag="NOA01_002419"
     CDS             2141..3118
                     /locus_tag="NOA01_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCI0633578.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="NOA01_002420"
     CDS             3128..3625
                     /locus_tag="NOA01_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCI0633579.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="NOA01_002421"
     CDS             4001..4282
                     /locus_tag="NOA01_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633580.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="NOA01_002422"
     CDS             4284..4700
                     /locus_tag="NOA01_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633581.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1757 a          817 c          786 g         1428 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtcgacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DAIGOK010000149         4755 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.151, whole genome shotgun sequence.
ACCESSION   DAIGOK010000149 DAIGOK010000000
VERSION     DAIGOK010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4755
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.151"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(82..1890)
                     /gene="pepF"
                     /locus_tag="NOA01_002423"
     CDS             complement(82..1890)
                     /gene="pepF"
                     /locus_tag="NOA01_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCI0633582.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(2066..3064)
                     /locus_tag="NOA01_002424"
     CDS             complement(2066..3064)
                     /locus_tag="NOA01_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HCI0633583.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(3290..3943)
                     /locus_tag="NOA01_002425"
     CDS             complement(3290..3943)
                     /locus_tag="NOA01_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HCI0633584.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="NOA01_002426"
     CDS             complement(4275..4673)
                     /gene="spxA"
                     /locus_tag="NOA01_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCI0633585.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT         1394 a         1001 c          743 g         1617 t
ORIGIN      
        1 taaaaaaata cgtcgactcc cttacaagag agccgacgta tttttttcgt ttctattcga
       61 gaaaatcaaa aatagaaagc cttataaacg atcgatcaat tcttctaatt catttagacg
      121 ttgttcaaac atcgacattg catcttcaat atatgcagcc tgtgtcatat caactcctgc
      181 ttttttcatt acttccacag gataatcgct gttacctgct tttaggtaag ccaagtagtt
      241 ctctagtgcc tctggttcct ggtttagtat tttcttcgcc agtgctgaag cagcagaaaa
      301 gccagtcgag tattggaaaa catagtaatt ataataaaaa tgcggtatcc gtgaccattc
      361 gaatttaatt tctgggtctt cttcgactgc tggaccataa tattttgcat tcaatttacc
      421 ataactatca cttaggtatt cactagtcaa tggcacacct ttttcatctt ccgtatgcat
      481 gaaatgctcg aattcagcga attgtgtctg gcggaaaact gttcctttaa acccatccaa
      541 gtaatggttg agtacataag cccgtacacg agggtctttt tctgtttcca ataaatactc
      601 cgttaggata ttttcattcg ttgtcgaagc aatttctgct aaaaagatgg agtagtcgcc
      661 gtacacataa ggctggttcg aacgagtgaa ataactatga acactatgtc ccatttcgtg
      721 gacaagcgta aatagctgat ccagcgtatc atgccaattc aataaaatat atggatttgt
      781 gtcatagctt cccgaagaat aagcaccgct tcgtttccct ttattttcga caacatcgat
      841 ccaacgttca gagaatgctt tttctacgat ggccatgtat tcttcaccca ttggtttcag
      901 tgcttctaaa gctttttctt ttgcttcttc gtacgtaaag gtaattggag cttcccctaa
      961 gaccggtgta taaagatcat acatgtgcag tttttccact tctaataaac gtttccgtaa
     1021 ttccatgtat cgatgtaaca aaggcaaatg cttgtttacc acgtctacca aagtatcgta
     1081 tacactttca ggaatatgat tattgctcaa agacgcttct ctggcagagc tgtaattacg
     1141 gactttcgct ttaaaattat gtccttttat atgtgtgcct aaagtagaag caaatgtatt
     1201 tctaaattgt tcgtaaacac tgtacaaccc cttaaatgct gcttcgcgca cccttcgatc
     1261 cgtgctttct agcaactgac catacacgcc atgagataat tggactattt caccattttc
     1321 tccttcaatc gttggaaaaa ctagatctgc attattcaaa acagcaaatg tatcacttga
     1381 agcatcaaag atttcacctg ctccagcaag aagagattcc tgctcagcag ataagacatg
     1441 ggctcgatta tctactattt gctggatata atggcgatag acttccaatt ttggttcttc
     1501 tttgaaatat tgccaaatct ggtcatctga caattgcaat atttctggtt caaaccagga
     1561 aacggcttca ctaactttcg aaaacagact gcttgctctt gcataaagcg cttggtaatc
     1621 tgtatttcca gtgtcttgat cgtttttaag gtgcgcatat acataaatga cttcagtttg
     1681 gcgataaacc ctcaatacga attcaatcgc atttaaaaat tgagaagcgc cttgatcaag
     1741 tgttcctttg tgtttttcag attgttttaa ctcttctgat aattccaaat atttctcatc
     1801 aaactcttgg tcgcttgaaa agatcttggt caagtcccaa gttaaatttt caggcaattc
     1861 ttctcgtttt ggcaactgct ttacttccat gaaatccact ccttttcttc ccttagctct
     1921 atctatcaaa atagatcata gtctaacatc cacatgtcac agcatctaac ttttctactc
     1981 attcatgtgt ctgcttccat caattttagc ataactaaat cttgttttac atagaaaacc
     2041 gattcctagc ctatttttat acttactatt ttttcaaaga agacaagtgt atgcattcgt
     2101 taaaaaactg ctgatagacc atctcttcat caatcatagg aaaaagccat tcccttttat
     2161 gttcttttat ttggcaaagc catgagttga aatcaggaac tttctggttt tccaaggctt
     2221 cttcgtacaa cattcgataa agaaaaacct gttcttgtaa ataaaagaaa aaccgacttt
     2281 tttgatagat ccacgatgat agatgcaaaa gatgtttatg acgcaagtag cattgttctt
     2341 gtatggatac tgtattcttt tgcttgtgaa taagttggcg tgtcagccat ttcttcccat
     2401 cttcactcac tttttctatt tttttcattt ggttcatttt aatttcgtta ctagtgaata
     2461 tttctttaag gtccctacta gaaaaagtcc agcagctact gcaaaaggaa atatgccctg
     2521 atacagtctc tgtaatatga tgatacaaat aaagcttttg tttcttgaga tcagctttcc
     2581 agcaatgcac tcctcttttt ctattatagt agcagcaact tttttctatc aatgagaatt
     2641 gcgattgtcc aagaaaagaa ttaccaagta tccaccatac ttggtaactg ttattcaaat
     2701 aattttgggt tctttcaata aatctctggt gagacagtct agaacattgg atttctattg
     2761 ctagattcgc caacaaaata tccggccttt gccgcaacct cggcaaatag gcttccaaaa
     2821 atactggttc tactgactgt cccagccaat ccatgaagca ttcctttaac tgtaagtgct
     2881 cttttgattc tctttcacta aagaccgaac agttatctga tttttgatgg gcaaaatgag
     2941 cacatttttt ctttcccttt ttcaaaatca ctctttttcg acagccacga caataatatt
     3001 tctttccttt ttctgcatgt ttagcaaaac agcacttatt atattcattc attgcaagca
     3061 gcatttttct ccctctgaac ttatttacgt ctaagaattt atttacacga aaaaagaggg
     3121 cgtgaaaaag ctattctgca tcaagtaata agaacagcta aacaaaaata gttcccaata
     3181 tttttcgttt ggttgggctt gttaccgcag atgcaagctt ttgaacacat ttatacgaaa
     3241 aaaagactgc gacacaattt ttgtcacagt ctcttactat aatgctgatt tatacaaaat
     3301 aaaatttcgt tcgctcgata gcattacgcg ccatcagaca ttgtccatat tcattcaatg
     3361 tttctggtgt acgatcgctc ttttcagcaa actccaaaat tcgagcaatc tgattctcta
     3421 catccgcttg gttgagattt tccatccaga agtgaacgac taggtaatag cgctcttcga
     3481 ataaatacaa atcagtccaa gcagactcta gttcgacatc tgttgcaagt tggatcactt
     3541 gttcaaaatc ttcaaaagca aaagttgctt gtctttcctg ttcttctagg gcgcttccag
     3601 ataattcttc ctccggatcg ctgccaataa tctgctgacg aagaaagtca ctcacatctt
     3661 ctgaatcaac agtagaatct tctgattgag aaatttcttc atccggaaga tttttactga
     3721 taaacagttc taatccgtct ccttttggca aaacttggaa agtgacagct tcgcttcctc
     3781 ggaactcctc ttcgatatca acttcttcca aaatactata aaagaaattt tcgatttctt
     3841 tgtgattgcc aagtaaatcc aaaaatgaaa cgccccgttc ctctaaatct tcatgaccaa
     3901 ttagtacacg gatcgtattt tcattaatat gttccatttc cattttgcta cacctcactt
     3961 tgccacaatg tttaatatag attcattgta gcgaaatcag cttaaatgta aagaaaaaaa
     4021 atctcgctgt taagcgaatt taataaaacc agcggatttt tcaacctttc aagctatctt
     4081 atttaactat aacatttttg gcagacgaat actatatagc cgacttttca gctaattttc
     4141 cctctctatt tttcgtgcaa tcatcatctt ctactacctt tttatacatc taatcaagaa
     4201 tactttttgt aaaaacaaaa aaacttggaa aaactatctt tgtaatagtc ttttccaagt
     4261 ttcgtattat ccttctataa tccagccatc aattgtgctt gacgtaattc taattgtcgt
     4321 acatcgcgcg gtaggaaccg acgaatttca tcttcattaa aaccaacttg cagacgttta
     4381 tcatcaatca tgattggacg gcgaagcaat ccaggattgt tttgcacaag ttccaataat
     4441 tcttgtaacg gcagatcatc taaatccatg ttcagttttt ggaatacttt ggaacgtgtc
     4501 gagatgattt cttctgttcc gtcttctgtc atttgtaaaa tcgcttttaa ttcagaactg
     4561 ttcaatggtt ctgaaaaaat attacgttca acaaatggaa tctgatgttc ttgcaaccac
     4621 gcacgagcct ttctgcagga agtacaactt ggggaagtat ataatgttaa caacgtgcac
     4681 cactcctttt cgtgataaga atcacttttt tgtaacagaa gatcataata atctcccatc
     4741 tgtttctatt ataca
//
LOCUS       DAIGOK010000150         4702 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.152, whole genome shotgun sequence.
ACCESSION   DAIGOK010000150 DAIGOK010000000
VERSION     DAIGOK010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.152"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..183
                     /locus_tag="NOA01_002427"
     CDS             <1..183
                     /locus_tag="NOA01_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HCI0633586.1"
                     /translation="QRKTVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLS
                     RTQRRFEGTLRYSLGY"
     gene            270..1124
                     /locus_tag="NOA01_002428"
     CDS             270..1124
                     /locus_tag="NOA01_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCI0633587.1"
                     /translation="MENSLYLVKEPNTHLRDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINEQKYYLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGELVEYY
                     LKEIGVGKENLTFKVENLNSFKGIIFETLSYDQDNLISELSRQTQVYKFLELLLNSAS
                     YLKEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKSDMGISI
                     KEYISEIKLSIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGAYRKRRL
                     GLGIINGE"
     gene            1221..3470
                     /locus_tag="NOA01_002429"
     CDS             1221..3470
                     /locus_tag="NOA01_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCI0633588.1"
                     /translation="MYKNNEMNIIYRSENQQFHLFNQEISYIFKISDDGKLLHLYYGE
                     NLPDNDYTHLIEMHHRPMTTYRKENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLTDFIYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3473..4648
                     /locus_tag="NOA01_002430"
     CDS             3473..4648
                     /locus_tag="NOA01_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCI0633589.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTRVTRGAS"
BASE COUNT         1646 a          676 c          863 g         1517 t
ORIGIN      
        1 caaagaaaaa cagttgagac tgttttttct tccttagaaa aattaggttg tcaaaatttc
       61 aactcacgtt ctgttaaagg gctagagagt agatttgaaa gcatattact ggcttacagt
      121 gttctattaa gtcgaacaca acgacgtttt gaaggaactt tgagatattc tttaggatat
      181 taaaattaac tagcaccacg ggtgatatta tgtaataata taaacatgat gaaacaaaaa
      241 ttatccaaat ctaacttagg aggactaata tggaaaattc tctatattta gtgaaagagc
      301 ctaataccca tttgagagat tgttacttta atttttgtgg atactcaaaa acagaaccta
      361 gacatagttt tggaccagct attcgagaac agtacttaat acacatagtc ttagaaggtg
      421 aaggttatta ttcaattaat gaacaaaaat actatctgaa aaaaggacaa ggttttgtga
      481 ttccaccagg agtatctact ttctatcaag cagatgaaaa aaatccttgg tcgtatgtat
      541 ggttgggatt aagtggagaa cttgtagaat actatctaaa agaaatcggt gttggaaagg
      601 agaatcttac ctttaaagtc gaaaacttaa attcttttaa aggaataatc tttgaaactt
      661 tatcttatga tcaggataat ctaattagtg aattatccag acaaacacaa gtttataaat
      721 ttttagaact cctcttgaac tcagcgagtt atttgaagga agggataatt actaaaaaga
      781 tgaatccttt tgttcgtcaa acaattgaaa taatacgtga gaatgcgtat caaaaattat
      841 ctgttaaaga tattgctgtc agtctatcga tacacgaaaa ttatttatca cgattgttta
      901 agagtgatat gggaatatca ataaaagagt atatcagtga aattaagctt tcgattgcaa
      961 atgacctact atcatcaact gaatattcaa ttcagcaaat cagtgataag ataggattct
     1021 caacagttca atctttttct aaagctttca agaaggccaa tggtgtttca cccggggcgt
     1081 atcgaaaacg taggttaggt ctaggaatca ttaatggtga ataaactaaa catttgattt
     1141 tgataaatat tcgttacttt aaacgcttac tatttatgta agcgtttttt ataatgcaat
     1201 aaagaagtgg aggagagcgt atgtataaga ataatgaaat gaatattatt tatcgatcag
     1261 aaaatcaaca atttcatttg tttaatcagg agataagtta catttttaaa atatcggatg
     1321 atggtaaatt actgcattta tattatggtg agaacttacc agacaacgat tatactcatc
     1381 taattgagat gcaccatcga cctatgacta cttatagaaa ggaaaacgat ttattatact
     1441 ccctagagca cttgaaacaa gaatttcctg aatatggtac aacagatttt agacatcctg
     1501 ccatttcact actacagaaa aatggctctc gtctaacaga ttttatctat caagggtacg
     1561 aagtggaaga aggaaaacca aaattagaag gtttacctgc tacctatgtt gataataatg
     1621 atgaaagcaa aacattgaaa gtttatattt tagattcagt tacaaatgtt caagtagaat
     1681 tattatatac aatttataga gattatcctg ttattactcg tagcgcgcga gttatgaata
     1741 aaggaaaaga tattcaaata atcgagaata ttagcagttt atcattggat ttacccgatg
     1801 caaattatga atggcaacaa ttatctggtg cttggggtag agaaagaact attaagtcgc
     1861 gtactttaca acaaggaatt caatcaatcg aatctactcg aggtatttca agccatcagc
     1921 ataacccatt tgttacttta aaaagaaaaa atactgatga aaatcaagga gaagctattg
     1981 gtgcagcact agtttactca ggaaacttct taattcaagc agaagtagat acatgggatg
     2041 ttactagact acaagttggc atcaatccat ttggatttaa ttggaaactc aatccaggag
     2101 aaatatttac agcaccagaa gcgattctgg tttattcaag taagggttta aatgccatga
     2161 gtcaaacttt tcatcaacta tttagaaaac gcttagctcg tggtaagtgg cgagaaaaag
     2221 accgtcctgt cttaattaat aattgggaag caacatattt tgattttgac gaagaaaagt
     2281 tggtaaatat tgctaaaaaa gcatttgatg ttggtattga attatttgta ttagatgatg
     2341 gctggtttgg agaaagagaa aacgattgtg caggattagg agattggcat gtaaatccta
     2401 aaagactacc tagaggaatc ggcagccttt cagaaaaaat tcgtagtttt ggtatgaaat
     2461 ttggtttatg gtttgaacct gaaatggtga ataaggatag tgagttatat cgcacacatc
     2521 cgaattggat tatttctaca cctaatcgaa aacctaatca tggtaggaac caatatgtat
     2581 taaattttgg gttagatgag gttgttgaaa atattttcga tcaaatgtgt aaaatcattg
     2641 atgaatcaaa cttagattac attaagtggg atatgaatcg tccattgact gatgtatttg
     2701 acagtcactt gtcagcagat caacaaggag aagtattcca cagatatgta ctaggcgtat
     2761 atcgcttata tgaaaaactt ataacaaaat atcctaacat actatttgag tcttgttcat
     2821 caggtggtgg gcgctttgat cctggaatgc ttcactatgc acctcaagct tgggctagtg
     2881 atgattcaga tgcaattgaa aggttaaaaa ttcaatatgg aactagtatg ctgtatcctc
     2941 tttcgtctat tggtgctcat gtatcaattg ttccaaatca tcaaacaaac cgtataacac
     3001 ctataaaaac tagaggaaat gtagcttttt ttggtgcatt tggatatgag cttgacttaa
     3061 atgaattaag tgaaggcgat ttgaggattg taaaagaaca aattgccttt tataaaaaat
     3121 atagaaccat aattcataat ggtacttttt atagactatt atcaccattc gatgatgata
     3181 atcaaaccgc gtggatggtt gttagtcatg ataagaaaac ggctattgtt gctcattata
     3241 aaacattaaa tgaggtaaat gctccttacc gccgactgaa attaaaaggg ttagataatg
     3301 acggacttta taatgtagga caaaatagtt tgcgatctgg aatggaattg atgagagcag
     3361 gactagtctg ttcagatgca tctagtggac aaataaatga tcagaatcga cgcccagaaa
     3421 catttgattt tgattccaag atttgggtta taacacagga ggaaaagtag agatgaataa
     3481 ccaaaaaagc ttacttcaaa aaggagcaat cttaagtatt tcactaatgt taacaagctc
     3541 tcaagcaatt aatggtgtta ttccacaaat gaaacagtct ttggggatat cacaaagtca
     3601 aagtgaactt ttaggaactg caccaagtat aactgttatt ttgtttatct tattatctag
     3661 ttactttgcc aaaagaatag gaatgaaaaa gacaataatt ttaggtttat tattagctgg
     3721 tataggagga attattccag tatttacagc taccttccaa accgttctta ttagtcgagt
     3781 aatattaggt gccggtttag gagtatataa ttcattggct gtgacatata tcagctcttt
     3841 atactctggc aatactagag caacgctatt aggtattaga aattcaatgg aagctttagg
     3901 tcagacaatc cttatttttc ttgctggagt tttagtgaac attaattgga cggcttcgtt
     3961 tttagtctat gcaattgcgt ttcctgtagc tctcttattc gcgctaaaag ttcctgaaat
     4021 tagagatgaa aatagtgaca tacctgaaaa tcatgtaaaa gaaaagatga atccaatggt
     4081 ttttgcccta gttctatttg caatcttact cgtaatgaat agtattgcta tttctgtacg
     4141 atttgcatca attgcaactg aaattaaagg ggtaaacttt aatgctagta actatttagc
     4201 tctaatgcct atcttaggaa tactagcagg atttatgttt ggaccaataa ataagtggat
     4261 aggaaaagga accctatact tgggaattat attctttata atttcaaatt tgttaattgc
     4321 tgtgtcaaac ggtaatatga cgttcctgct aacgggttta tttttatcaa gtataacagg
     4381 atcatggtgc ttcccattta tctttagtaa cttagataaa gtcactacaa agaatacaat
     4441 taattttgct acttcattga tttttattgg ctgtaatatt ggtaatttta tagctcctat
     4501 tgctatgcaa ttagcacaat ttctaacaag aagtacaaat ttgactgctc cattttttgt
     4561 attcgcagga atatttattg tgatcctatt tgtaacattt tttgcgacaa ataaaaaaaa
     4621 tactagagta acccgtggtg ctagttaatt ttaatatcct aaagaatatc tcaaagttcc
     4681 ttcaaaacgt cgttgtgctc ga
//
LOCUS       DAIGOK010000151         4653 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.153, whole genome shotgun sequence.
ACCESSION   DAIGOK010000151 DAIGOK010000000
VERSION     DAIGOK010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.153"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(613..1431)
                     /locus_tag="NOA01_002431"
     CDS             complement(613..1431)
                     /locus_tag="NOA01_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCI0633590.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1592..2281
                     /locus_tag="NOA01_002432"
     CDS             1592..2281
                     /locus_tag="NOA01_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCI0633591.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2295..3797
                     /locus_tag="NOA01_002433"
     CDS             2295..3797
                     /locus_tag="NOA01_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCI0633592.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            3810..4286
                     /locus_tag="NOA01_002434"
     CDS             3810..4286
                     /locus_tag="NOA01_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCI0633593.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKYGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(4383..4493)
                     /locus_tag="NOA01_002435"
                     /pseudo
     CDS             complement(4383..4493)
                     /locus_tag="NOA01_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1559 a          666 c          823 g         1605 t
ORIGIN      
        1 tctgaagctg tattaaatac agatagatta atataaaaat caaaaggatc ataaactttt
       61 aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttatctca taacattgaa
      121 gtgttttgca attttagcat tgaaacactt ttttaatctt caacgttgtt cttataaaca
      181 agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt tatagaaaac
      241 attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc agtaggttcc
      301 cgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt atttaagaga
      361 ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta cacgtttttc
      421 tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc aagaaccgta
      481 ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa taagctatca
      541 agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta ggtttagcta
      601 agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt tttctcttaa
      661 ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat cacaaatata
      721 aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa tggctgtttg
      781 taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta tagcaataat
      841 ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg ttttcccaga
      901 taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt gggcttgaaa
      961 atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat tttctaaatc
     1021 aagactagta tttccactag atcccactcc aaatatatat atgttttttg attgagtgat
     1081 cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg aatagagagc
     1141 ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat gttctttttt
     1201 ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag aaaaaccaat
     1261 tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct taatatcact
     1321 catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga cttttttttc
     1381 agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca tacaaactcc
     1441 tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact cctaaatact
     1501 ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag ttgttatcca
     1561 aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt tagatcttat
     1621 ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt acaaagagga
     1681 agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag ttggtatacg
     1741 tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac caatgattgg
     1801 aattattaaa agagattatc caccagaatc tccattcatt actgccacta tgagagaagt
     1861 agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc ttagaaatcg
     1921 acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat atcctcatca
     1981 gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata aagcaggaat
     2041 agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac aagatgggcc
     2101 agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg ctgagggaag
     2161 gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag gtattgttgt
     2221 gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag cattaagtta
     2281 gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc atgttaccaa
     2341 ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta acaaatgaga
     2401 gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt ttaagtatta
     2461 tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca attggtattg
     2521 ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga gtagcttatt
     2581 tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa gatgcaacac
     2641 tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt ttacacaatc
     2701 gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca agatttattc
     2761 cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta atttggccac
     2821 caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt agtttaggaa
     2881 cttttttata tggtttttta ttacgtttaa caggtgctgt tggactacac cacaccattt
     2941 accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga tcaacaattg
     3001 ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact ggtctgttta
     3061 cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttggg ttaccagcag
     3121 catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat ggtgggcttt
     3181 attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt gaatatatgt
     3241 ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga ttatcttttt
     3301 attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt ttgatcgact
     3361 ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa ttaattcctt
     3421 ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc aaaaaattta
     3481 aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa gtaataaaca
     3541 aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt ggaaaagaaa
     3601 atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttaca aatggggaga
     3661 ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa gtgaagggtg
     3721 gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc aatcaaatat
     3781 taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag aaaaaacaag
     3841 aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta aatgatcctg
     3901 tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt tcaaaaattt
     3961 atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct attaatataa
     4021 aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt gaattacaag
     4081 gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa acctcgttgg
     4141 ttgatgtgga ttttgacaaa ataataaaat atggtaaaga tacttctgtt atagttgttt
     4201 ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt gctcatggga
     4261 attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa aataagctaa
     4321 ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt ctgttcattt
     4381 ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt aaagttataa
     4441 aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt caaatataaa
     4501 atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg tttactcaac
     4561 ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga accttagcac
     4621 tatataccaa atatagtaaa atgctgaaag atg
//
LOCUS       DAIGOK010000152         4623 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.154, whole genome shotgun sequence.
ACCESSION   DAIGOK010000152 DAIGOK010000000
VERSION     DAIGOK010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.154"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(52..522)
                     /locus_tag="NOA01_002436"
     CDS             complement(52..522)
                     /locus_tag="NOA01_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCI0633594.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(681..1139)
                     /locus_tag="NOA01_002437"
     CDS             complement(681..1139)
                     /locus_tag="NOA01_002437"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCI0633595.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1310..2455)
                     /locus_tag="NOA01_002438"
     CDS             complement(1310..2455)
                     /locus_tag="NOA01_002438"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCI0633596.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2468..3385)
                     /locus_tag="NOA01_002439"
     CDS             complement(2468..3385)
                     /locus_tag="NOA01_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HCI0633597.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3813..4376
                     /gene="efp"
                     /locus_tag="NOA01_002440"
     CDS             3813..4376
                     /gene="efp"
                     /locus_tag="NOA01_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCI0633598.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
BASE COUNT         1448 a          844 c          874 g         1457 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tcttttttta tttttattaa gttaaagaac
       61 ggattcaatt gcagcggtca ttttcaatgg agaagtttgt ggtgcaaatc gttcgattac
      121 tttgccattt ctatcaatca aaaatttagt aaaattccat tttattgctt tagtgacagt
      181 accaggagct tcatttttaa gataagtaaa aagaggatct gtattttctc catttacgtc
      241 aatcaattga tgcataggga aggtaacgcc ataagtggtt ttacaagctt ctgctgcttc
      301 agtcgctgaa tctagctctt gtttaaattg attggaagga aagcctaaga taaccagacc
      361 ttgtgtctga taagcttggt aaagttcttc taattgttta aattgaggtg ctagaccgca
      421 ttttgttgca gtgttaacaa tgattatcgg tttgcctgca tattttttca atgaatagac
      481 agtgccgtct tctaaagtag tttcaaaatt ataaatagac ataaagtttt ctcctttcta
      541 ttttatataa taaaaagagc gctaaactga aaactagcta tttgttttcc cgtttagaac
      601 tcttttcaga caataaatat ttaataaaat acgaagataa gatagctata ttgcttctca
      661 tctttacgtt cttttagaaa ctatgcttct acttcagacc attcatcgcg ttttgctaaa
      721 aattcacgag ccaaagcttg tgcaccttct agtgtatggc tggcagccca gccacattgt
      781 gtttcattgc ttgcaggtac ttcagtagct tcaagaacat ctttcattgt tgcttcaaga
      841 acatctaaaa tgtcttcgta gctttcatga ttcaatacag tcaagtaaaa gcctgtttgg
      901 catcccattg gagaccagtc tacgatgtaa tctgcgtgat tgcggattag ttcggcagtc
      961 aagtgctcaa gtgaatgtag acttggcatg tccatgtgtt ctttatttgg ttgtttaaag
     1021 cggacgtcgt atttaatgat gacatcgcca tgcgcacccg tttttcgatc agctacacgt
     1081 acataaggtg ctttgacttt tgtatggtca agattaaaac tttcgacatt cattctcatt
     1141 tgtaagactc ctatctgttt gttcattcgt tttgatgctt ttcactaaag aattatttag
     1201 tgaaatttac aaaaacgtct ttctacttga ttctaacact tatcgtttcc aaggtgtagc
     1261 aatctgaaat attttttaga tttttctgct aagtcgttta ttgggacttc tagttcttga
     1321 ttctgtcaaa tgcctgagct aaatcgttta tgagatcctg accatactca attcctacag
     1381 aaagacggat caagccgtcc tttatgccag cctcttctcg tttttctttt ggaatagacg
     1441 catgagtcat gacagaagga atttccacta aactttctac gccacctaaa ctttctgcta
     1501 aagtaaatag ttggagtgat tcaacaaatg gaatagcaga ttcttcattt tttaaagtaa
     1561 aagaaatcat tccgctaaag ccgctcatct gctgcttagc aactccatgg agttcatgat
     1621 cgggtaatcc tggatagtag accttttcta ctgctggatg agaaaataga aaatcagcta
     1681 ccacaaaagc atttttttga tgttcctcca tacgaacact tagtgtcttg attccgcgct
     1741 gaagtaacca gctatcttgg ggaccaagta cggctccgat agcattttgg taaaatccga
     1801 tctgatcggc tagatcttta tggttagaag tcactaaacc agctactaca tcgctgtgtc
     1861 cgcctagata tttggtaccg ctatggacaa cgatatcaga accgagatct aaaggtcttt
     1921 gaaaataagg agtagcaaat gtattgtcag caataactat caatccatgt tgatgagcca
     1981 gagtggcact tttttctaaa tcagtaatct ttagaagcgg attgctaggt gtttcaagat
     2041 aaagtgcttt tgtattgggt ttgatggatt gttctatttt atcaagatta ctcgtatcta
     2101 tgatcgtgta ctcgagacca tttttagtaa gaactttgtc aaatagacgg aatgttcctc
     2161 catagacatc atctcctaat aagatatgat cgccagcttg aaaaagagaa aaaacggcat
     2221 gaatcccaga aagaccagaa gaaaaagcga atcctcgaac accgccttcc aaatcagcaa
     2281 tcagttcttc caatgcaaaa cgagtaggat taccagatct tgaatattca tattgtttag
     2341 gctggccgac gccatcttgc cgataagtgg atgtttgata aataggtaca ctgacagctc
     2401 ctgttgtggg atcttcactg atgcctccgt ggatcaattt tgtttgaatg tgcatgtttc
     2461 tttcctccta taagtaaatg ttttttgata aatagcggtc tgctgcgtct ggaaaaatcg
     2521 taacgacacg atgaccaggt ggtaaacgac gaatttctct taatgctgct gcaaaagccg
     2581 caccactcga acttccaact aatagtcctt gttctcgtgc taactgccta gtatagttaa
     2641 acccttctac atcagagatt gtctcgatct gattgattga aaggggagaa agaaacggcg
     2701 gaatgaattc tacaccgata ccctcaattt cgtgaggggc tggatcacca ccatttaaca
     2761 cagagccctc tggttctaca ccgattattc gaatgtcggg atatttttct ttcaaaaagg
     2821 tagaagttcc agcaaaggtt ccaccactcc caattcctgc tacaaaacta tggatatttt
     2881 ctttgatttc atgaaagatt tctggcccta acgtatgata gtaagcagca ggattatctt
     2941 tattttcaaa ttgaagaggc agatagctgt ttgatatctc ctctgcaagt tttttacttt
     3001 tgctgatggc accgatgatt ccttgctcac taggagaatg gatgatcttt gctcccaatg
     3061 ctttcatcaa ttgttgtttt tctaagctaa acttctctgg aacaacaaaa accgttctta
     3121 atttgtatgt gagtgcggcc agagcgatcc caattcctgt atttccggca gttggttcga
     3181 tgatcgtcgt tttttcattg atttttcctt gggcaaatcc ttctttgatc aaataactgc
     3241 ccaatctatc tttgatactt cctccaggat ttaaatattc tagttttgca taaatagcag
     3301 aacctttagg catagaatag tgctgattgt caaagcgata aagcggtgtg gaaccaatag
     3361 cttctgtaat tgtcgagata atcatgtggg cctcctaaaa aaagataata aaaaaaggag
     3421 ctgactatta aatcagctcc ttgaaagaca tagagaaaaa ttgagtagat aaaaagataa
     3481 gttacctggt actcattcta tgaagggcaa ttaatagaca tgacaaatag tatgctgctt
     3541 ttatgcacca tacaacagca acgattcaat tgtttttggt tgctgactgt catgtgtatc
     3601 cctccaagta aaatttttat taacgatagc atgaaaaaaa ataaaagtca aaggcttgaa
     3661 agaaaaaaat atttcgatag catcgtttac ccgtgtttat ttttctgaca aataggcatg
     3721 agaccttaaa agatagagaa tactctagtt ttctggccgg ttgtctgata caataaagtt
     3781 gattatgata aagaaaagaa ggagcaaaaa taatgatctc agcaagtgat ttaagagccg
     3841 gtatgacttt cgtacaagac ggcaaactga ttaaagttct ggatgcaagc caccataaac
     3901 ctggtaaagg aaatacagtt atgcgtatga aactgaaaga tgtccgttca ggtgcaacat
     3961 acgacacaac gttcagacca gaagaaaaat ttgaaaaagc acatatcgac acaaaacctg
     4021 tacaatattt gtacacgatg gatgatacag ctttcttcat ggatttggaa acatatgagc
     4081 aatatgaaat tcctgtagaa acagtagaag aagaaatgaa atatatccta gaaaacatgg
     4141 aagtaaaaat ccaatttttc ggtagcgaag tcatcggcgt tcaattacca acaactgtcg
     4201 tattacgtgt tgtggaaaca caaccttcca tcaaaggtgc aacagtcact ggttcaggta
     4261 agccagcgac aatggaaact ggcttagtag tcaatgttcc tgacttcatc gaaacagatg
     4321 aattgttgga aatcaataca gcagaaggtt cttatgtaaa acgtgcgtca aaataattcg
     4381 ctaacggatt gataagaatt tgaaaacaga gcttgacctc ttgaaaggca gatcatcgga
     4441 tctgcctttc aagaggtttt ttgtggaatc agcaaattga aaaataaaaa agtatttgat
     4501 tttatttata ttttgaaaga tgaatggcaa aagctagaga aagaaaaaac tgcacttatt
     4561 tatgaagaaa ttgaaatgat tcataaaatt gaaaatatcc gaactattcc gaactatatt
     4621 gaa
//
LOCUS       DAIGOK010000153         4527 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.155, whole genome shotgun sequence.
ACCESSION   DAIGOK010000153 DAIGOK010000000
VERSION     DAIGOK010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.155"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(<1..437)
                     /locus_tag="NOA01_002441"
     CDS             complement(<1..437)
                     /locus_tag="NOA01_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCI0633599.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAVRIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVY"
     gene            751..1173
                     /locus_tag="NOA01_002442"
     CDS             751..1173
                     /locus_tag="NOA01_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633600.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1183..1386
                     /locus_tag="NOA01_002443"
     CDS             1183..1386
                     /locus_tag="NOA01_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633601.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            1716..2333
                     /locus_tag="NOA01_002444"
                     /pseudo
     CDS             1716..2333
                     /locus_tag="NOA01_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            2507..2716
                     /locus_tag="NOA01_002445"
     CDS             2507..2716
                     /locus_tag="NOA01_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633602.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            2923..3123
                     /locus_tag="NOA01_002446"
                     /pseudo
     CDS             2923..3123
                     /locus_tag="NOA01_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3223..4524)
                     /locus_tag="NOA01_002447"
     CDS             complement(3223..4524)
                     /locus_tag="NOA01_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCI0633603.1"
                     /translation="MENSAFIMKLNDILSPIAAKIGNQRHLKAISSGMMFGLPFIVIG
                     SFFLIFANPPINIDKYNPDTANIFMKFMASWKDFAVANYDLITAPYNLTMGIIGFISV
                     FGIAFSLANEYKINPSMNGMVASVIYLMVCTPVEEGAINLNFLGTNGLFVAIIIGLLV
                     VEINRLFEVKNIKLKLPDTVPPMVATFINMLVPLLTNIFIFYGINLIFLAATNKIFPE
                     AVMEVLTPATNIAGSLGGFLLIVTLGNILWLFGINGSSIIFPIVFTLGMAQTGLNAEQ
                     LANGETMTHLMNLQMFRISVLGGAGGTLGLVLLMMRSKVPEYRTIGKLSLVPGICSIN
                     EPVIFGLPIVFNPILAIPFLITPIISLLLTYFAQQLGWIGIGFIVDPSFTPFFAQAYL
                     SSMDWRNILFCLLLIVISIFIYYPFFKVMESNKTKLVDEKL"
BASE COUNT         1584 a          708 c          847 g         1388 t
ORIGIN      
        1 ttataaactc catttgcttt cgcaatttta atgccttgcg cctggcgtcg tttagattcg
       61 gtacgctctt gttcagcgat catggccaaa atttggatga tcagatcctt aatgaattta
      121 tccaggagag ggttgccaat tgcttcagcc atgatcggta gactggtgat gattagctgt
      181 acgtttttct tctttaggta attgactgaa gcgatgatct cgtcgtaatt tcttccaagt
      241 cgatcaatag cttccacaat aaaaatgtct tgatctcgga cgaaatccaa tgcttcttgg
      301 aaaacaggtc gatgagtaat cgttgcgccc gattgtttct cgatgaaaat tctcactgca
      361 ccgaattttt tcatttcttc aatctggcgt tgttcatttt gatcgatgga tgagacacgt
      421 atgtatgcaa ctctcaaaat ttttacctac tttctgcaac tatttaaaat acaccctaat
      481 catacacgat aaaaccagat ttttcaatgt tgtggtcgtg tctagttagg gtatacctta
      541 tatagacaaa aactaatgat ggaggaaata gtatgtgtca cttttctttt taaatggcaa
      601 tattttttgt aaataaatgt caatcaagaa aatttaaacg attatgacat taaaaatata
      661 ttttatgaat tttctagtat ataacttgat ttatctgtta agatttatta agaaaacgtt
      721 ttcttatttt tgatttcgag gtgatagaca atgaaaaata agtttatttt atctttaggt
      781 attttaacta ttggaattag tgtaactgga actacaattc ctacatttgc tgatagtaca
      841 aatgaaccaa ctactgtagt agataataac caagaattaa ctcctttaga taggaacgga
      901 atagccattg gtaacccaac taacgactta actgttactg aaaattattt aatagtttct
      961 ccattacttg ctcgagcagc ttctaaaacg atacgagtga caggaacgat gatttttgat
     1021 aaagttggtt ggccttctag acctcctcaa tcacggaatt ggaaagaaaa aagaaatggt
     1081 tattggtact atggaaaatt gtatttacaa agttataaat cagctggaaa caattggatt
     1141 gcgacttatt caggaacctt aactagaaag tagggatttt ttatgccaga atttgtgaac
     1201 acaccaaatt tttatcaaga tagcgatatt ctaagtaaaa ttaatttacc gaaagaagaa
     1261 tcagctttac aagttcagcg aactttacag gagacacaaa aaattcaaaa atcaatttct
     1321 aatctagaag attctgaaga aaaaagaaaa aaaagagacc aaaaaacata tcaaaaagaa
     1381 aaataatgat tttaaaaaaa gagtgtctag tcgacattct ttttttctaa ttgaaagtgt
     1441 tcataaaatc acccatttct ggacactttc tgtcattttt atatttaatt ggcacagttt
     1501 tttctaatta tttgtcatat tcatagtcaa ctatagctac aaaagcaact aattttggag
     1561 atagattgag atatttaaaa atctatcatg aaaaattagt tgcttttgtc acttagcgct
     1621 gaaaatttat gccatttatc tagaaaaatg acaacagagt tgggcaacag aatttaaaaa
     1681 tgatccggat ttcagaattt taaaggagtt ttattatggc gaaaattggg tatgctcgag
     1741 taagcagctt agattaaaat ttagaccgac aattagaatt attatcagag tgtgataaaa
     1801 tttttagtga taaagcaagt gggaaagata ccaatcgaga aggatttcaa gaaatgatga
     1861 aattcatacg tgaaggtgat attgtcgttg tgacagaatt aaaacgactt gaacgaaata
     1921 acaaagagct aacagaaacg atgaatttaa ttcagctgaa gaaagctaca ctggaagttt
     1981 tgaatttgcc agcgttaaca ggtatcactg atgataattt acgtcgatta cttaataact
     2041 taattatcga actatacaag taccaggccg aagaagaacg tcggtatatt cgcgaaacgc
     2101 aacaacaagg aattgcactc gctaaagcaa aagggaaata caagggaggt acgccaaaat
     2161 atcgtgagaa tgatccgcga cttcaattag catttaattt gtttttgact agatctacag
     2221 ataaagaagt tgaagagcta acagggatta atcgtagaac gtttagaaga tatcgaatga
     2281 aacatggtat tactaatgaa gttagggagc aaaaaaataa atctttcagt tgaggctaat
     2341 ataattttgc aagaaaggga tttttatgaa agaaattaga ttttcaaatc aaaatatgca
     2401 gaattactat aatgaaatta tgaatatgga aactagtgaa ttgaatttaa ttatagccga
     2461 aaaaaaagtt gcattggata ggaataaaaa cttattaaat gtagttatga tctgttatat
     2521 ttcatttcta atactaggtc ttccacaagc agtgtttcct gaaatgtttt ctggagactg
     2581 gggtattgtc agctattcta tattaacatt gattgctatg gttttaggta cttcatttta
     2641 taatgctttt gtaagtaagc atgttgagga atatgaagta ttaatgtact taaaagagaa
     2701 aaaaacaagt aattaatatg agaacaccac gcatagtttt tattttgaga tatcctagaa
     2761 tgaaacacat taagaacgaa agcaaagcaa aagacggcag aacttctact accttctaag
     2821 ttttttcata tttgtttgtt atgctataca tagatctgca taattagact tgcgctgccc
     2881 gttattgaag tttcaggcag cgctcttttt gggggatcga taatggaaga agaaaaaata
     2941 tttgagaaac gctggcagct agcatcaagt gaacaaagag caagatacaa taatttgatc
     3001 acctcctacc ccactgttga ctagacatat aaggaaaaga aatacttact ttggctgtgc
     3061 cagttagata tagatacctt tgaaacattt gaagtgatcc tagataaaat taaacagaat
     3121 tgatcagaaa caagcgaagc cttctctcgg ttcgcttgtt ttgctattta ttagttggct
     3181 cagggcaatt ctaagctcgt ttgaaagaaa accgaatgac gactatagtt tttcatctac
     3241 taattttgtt ttattgctct ccatgacttt aaaaaatgga tagtagatga agatgctgat
     3301 gacgatcaac agtaggcaaa agagaatatt gcgccagtcc atacttgaaa gataagcttg
     3361 tgcaaaaaaa ggtgtgaagg aaggatcgac gataaatcca atcccaatcc atcctagttg
     3421 ctgagcaaag taggtcagca ataatgaaat gatgggcgta atcaaaaatg gaattgccaa
     3481 aatagggtta aaaacgatag gtaatccgaa aatcactggt tcgttgatgg aacagattcc
     3541 aggaaccaat gacaattttc caattgtgcg atattcgggt actttgcttc gcatcatcaa
     3601 taaaaccaaa cccaaagtac caccggctcc accaagtaca gaaatacgga acatttgtaa
     3661 gttcattaag tgtgtcatgg tttcaccatt tgccagttgt tcagcattca aaccagtttg
     3721 agccattccc aatgtaaaga cgatagggaa aataattgac gagccattga taccaaatag
     3781 ccataggatg ttacccaaag tcacgatcaa taagaaacca cctagactgc cagcgatgtt
     3841 cgttgctgga gtcaggactt ccatcaccgc ttcagggaag attttgtttg ttgctgccaa
     3901 gaaaatcaga ttgatgccat aaaagatgaa tatatttgtc agcagtggaa cgagcatatt
     3961 gataaaggtt gctaccatgg gtggaactgt atctgggagt ttcaacttaa tattttttac
     4021 ttcaaatagt cggttgattt ccactactag caagccgatg ataattgcga cgaacaatcc
     4081 gtttgtccca aggaaattaa gattgatggc accttcttcc actggtgtac aaaccattag
     4141 ataaatgaca gatgcaacca tgccattcat ggaaggatta attttgtatt cattggcaag
     4201 ggaaaatgca atgccaaaaa cactaatgaa tccgatgatt cccattgtca aattataagg
     4261 ggctgtgatg agatcataat ttgctacggc aaaatctttc cagctggcca taaatttcat
     4321 gaaaatatta gctgtatctg gattgtattt atcaatattg atgggtggat ttgcaaaaat
     4381 cagaaaaaaa gaaccgataa caataaaggg taggccaaac atcataccgc tggatatagc
     4441 ttttaggtgg cgctgattac ctatcttggc tgcgatgggg ctaagtatgt cattcagttt
     4501 catgataaac gcagaatttt ccatata
//
LOCUS       DAIGOK010000154         4508 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.157, whole genome shotgun sequence.
ACCESSION   DAIGOK010000154 DAIGOK010000000
VERSION     DAIGOK010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.157"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(<1..738)
                     /locus_tag="NOA01_002448"
     CDS             complement(<1..738)
                     /locus_tag="NOA01_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0633604.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQNPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIE"
     gene            complement(749..1417)
                     /locus_tag="NOA01_002449"
     CDS             complement(749..1417)
                     /locus_tag="NOA01_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCI0633605.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(1548..2132)
                     /locus_tag="NOA01_002450"
     CDS             complement(1548..2132)
                     /locus_tag="NOA01_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCI0633606.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(2463..2696)
                     /locus_tag="NOA01_002451"
     CDS             complement(2463..2696)
                     /locus_tag="NOA01_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HCI0633607.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(2711..3658)
                     /locus_tag="NOA01_002452"
     CDS             complement(2711..3658)
                     /locus_tag="NOA01_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCI0633608.1"
                     /translation="MRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVVT
                     NSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETGG
                     NAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKNI
                     EKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYFS
                     KAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDTN
                     IPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(3658..4500)
                     /locus_tag="NOA01_002453"
     CDS             complement(3658..4500)
                     /locus_tag="NOA01_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCI0633609.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
BASE COUNT         1553 a          797 c          572 g         1586 t
ORIGIN      
        1 ttcaatcgaa ctattaaaaa tgtcaaattt ccttaatgcg ttattgtttt cagtttttaa
       61 caactgaata gttgtagttt ttacagttct atttttaaca aatagaactt gatcaattaa
      121 ttctttcaaa tagtaaaaac gatgttcagc tataacaata gtaaatcctt tattttttaa
      181 atcacaaagt aagtcagcaa gtttcttagt cgctttgaag tccaaatttg aagacggttc
      241 atccaatata attaatttag agtttagcga taaactacat ccaatagaaa ttttctgccg
      301 ttcaccactt gatagcgaaa aaatgttttt atctaatata tttgaaagat ctaataattc
      361 agctaattca ttgattcttt tatccatatg ctcttttgta aatccaaagt tttccatcga
      421 aaatactaac tcactcgttg tatttgttgt aaagaattgt cctctaggat tttgacttac
      481 gtagccaata ttttttgata tactttcttg tgttaaggat tgtagcgagt tattttcaaa
      541 taaaatttcc ccaaccaatt ttccgtcata aagtcttgga ataagtccat tcattaactt
      601 taatagtgtg gatttcccac tcccagaaga accagtaatc aatgtaagac tgcctttttt
      661 tattgaaaaa ttaaggtcaa caagcgcttg ttccttaaca tccttatatg aaaaagaaac
      721 attgttaaat tcaaacaatt ctttcacctc aaatcaaaat acttaagaaa aaaatagtaa
      781 cggaaaacag gatgatatag tctaataagc tcatgtttgt tttatagtaa gaacttcttt
      841 tcacaggaga ttctatcccc tttgttattg cattagcggt catttcttca gcaatcatag
      901 ttgcacgtat aagaacggga actaacagaa attccaaata ttgcagaggc tgaaaaaaag
      961 acaagcgaat gtttctcatt tttaaagatt ctttaatcat tcttatttca gaacgataag
     1021 tcggaaagaa tctgatcact actgtaatac ttaacattat ttttttcggt aaatgtattt
     1081 tttctaaaga ggataataat tcattgctag tatagacact aaccaacaac catgagatca
     1141 ttacgaccgg caacattcga aatacaatat ataaggatgt cccaaaaaat atagtaaaaa
     1201 actctatctt gatagcatca ataattttca ttactaaaaa cagaactgaa taaatcaaga
     1261 tgaaataaat acatgtcttt attttacgaa acatcaatac taaaactgta gcaataatga
     1321 aacaccccac ttgaaatata tttccctttc ctaaaaaaat aatgaccgga aaaaaaatat
     1381 ttaaagtaat caaggttaac ggattgattt ttgacactct ttcacctcca aaaaataaga
     1441 tcgcactgtt atcttaagct gacaaaaatg caccgttatt cagataacag tgcgatctta
     1501 tcaaaaagca taactgagaa caattctcaa tgcaagcttt tttttaatta actcagtaat
     1561 tcaatccacc caggaacaga aaattgatac aatcttctgg ttaatagcat agtcttttct
     1621 tttgaatagc tatgcttcaa tatatcatag aatgtagcgt agaatgagta aatgtagaag
     1681 tggatttctt cttcattaaa gactaaattt tgattgatca ttccgatagt ctttaactta
     1741 tctagacaac agactgtctt ttttgtcaca aatgatgtaa ttttttcaac aatatcttga
     1801 tatttggttc cagctgattg gaataaaacc aaatcgaata tacgtttatt ctcaaaaata
     1861 taattaataa atggatctaa ttcattgaag ttctctctcc aacttttctc tatatctata
     1921 ttttctaagt ttaagaggta tttggtaaac tcttcattat acatattaat aagcgcatta
     1981 atatgcggct gaataatttc ttcaaataac gcttccttag aagtaaaatg tttataaaaa
     2041 gcacccgtag ttacgcctgc ggtcaagcac agttgcctta tcttagtttg ctgaaatcct
     2101 tctttttcaa aatggcttag tccactttct attaattttt ggtgagttgt aatataatct
     2161 ctggtttcta tgggtacttc ctcctaataa gatgaattaa ctatattatc ttacacagga
     2221 cttgaagttt caatcgttaa cgtctattac tgaactcatt ccttagagct aaactaatga
     2281 gaaagagcaa aataactgat acaaaaagca atcctacaag aattaataga ctcatctaaa
     2341 tcattcacct tttctaaaat aatccgagaa agatttaaaa aaattatatc cagctattag
     2401 ggtaagtcct atcataatca ctccaatagc cattttctta tcctccctaa aaataaagtc
     2461 ttttacttaa aacctttttt gtaatcgtca ataaattttt tccctttatc agttaattga
     2521 aatcctatat ctaaatagtc ctcatatcta agtttagcca gcatctcact tacagatgct
     2581 ggactaacac ttagaacttt tgctaaatca ttatttttta atttcttttt tctttttgat
     2641 aattcctgaa ttgctagtaa ataaattagt atagtagtcg ttttaatttg taccatacct
     2701 accctccaaa ttatttcgaa aggccttcag caattttatc caggttccat ttcatcatcg
     2761 catagtaact atcaccatct tgtccttttt ctgcaattga atcagtaaaa atcgttgaat
     2821 agataggaat attggtatct tttgatactg ttttcattgg tctatcgtcc acactgcttt
     2881 ctacgaataa ggagggaact tttgttgtgc gtaatttttc aactaagtgt tttatttgat
     2941 ctggtgttcc ttcttcttca gtgttgattt cccaaatgta agcagaaggc acattatacg
     3001 ctttcgagaa atatttaaag catccttcac ttgtaacaat cattttttta tcattcggaa
     3061 ttgaagcaaa tttagattta gcttctttgt ctagagaatc caatttttca atatacttat
     3121 ctagattttc tttatagaat tttttattat caggatcttt ttccgctaac tgtttttcaa
     3181 tatttttagc gtaaataata ccgttttcta aatttaacca agcatgggga tcttccttcc
     3241 ctttctcact ctgaccctct aagtaaataa catctatgcc atcacttgct gcaaaatagt
     3301 ctttgttttc ctctttgttc gcatttttta ctaattttgt aaaccaagca tttcctccag
     3361 tttccaagtt aacaccgtta taaaaaatca aatctgcttt tgaagttttt tgaacatctt
     3421 caggcaaagg ttcatattcg tggggatctt tcccaatagg tacgatactg tgtaaatcaa
     3481 ttttatcttt tgctatattt tcagtaatat ctgctaaaat cgaattagta actactacct
     3541 ttaatttttc gttcgaagcc actgtatctt tgtctttact atttgttgaa catgccccga
     3601 atataaaaag agcaatcaaa atccccagac ttcccagtac tgctttccat tttctcatca
     3661 taactttctc tccttctttc ttaaaaattt ttgttttggt gagattagaa aactaattac
     3721 aaataagaat gccgctgtta aaacaatact tgatcctgct gctatgttaa aactataacc
     3781 aatgaacaaa cctagcaatg atgagcacgc tccgactgtc gccgaaataa atatcatagt
     3841 ttttaagctg tttgcatata aatatgctgt agcagctggt gtaattaaca ttgcaacaat
     3901 taatatagtt cccacacttt gcatagctgt tactgctact aaagttaata gaatcattaa
     3961 tagataatga taaaaactga cattcatccc cattgctttt gccattaatg gatcaaaaga
     4021 agtgattaat aattctttaa aaaatagaac aactgtaacc aacactaaaa cagaaacgcc
     4081 aattgtaatc cacttatcaa tatcttgtac agctaaaata tttccaaata agatatgaaa
     4141 taaatccgtt gagctatttg caactcctat aaggataact cctaatgcta aaaaagaact
     4201 gaaagtaata ccaattgctg tatctccttt tattacacta ttgcttttta taaaagtaat
     4261 aatgatagaa gctaatagac caaatacaat cgcaccaata aaaaaattaa tacctaaaat
     4321 ataagaaatt gctactcctg gcaaaacagc atgagaaata gcatcaccca taagagacat
     4381 tcctcttaaa ataataaaac aacctactgt tccagaaaca atccctataa ctacagatgt
     4441 aattagagca ttctgtaaaa agtgaaagtc atacagacca ttaataaaat tttgaagcat
     4501 attattcc
//
LOCUS       DAIGOK010000155         4461 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.158, whole genome shotgun sequence.
ACCESSION   DAIGOK010000155 DAIGOK010000000
VERSION     DAIGOK010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.158"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            574..1008
                     /locus_tag="NOA01_002454"
     CDS             574..1008
                     /locus_tag="NOA01_002454"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633610.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
     gene            2298..3221
                     /locus_tag="NOA01_002455"
     CDS             2298..3221
                     /locus_tag="NOA01_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HCI0633611.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            3237..3776
                     /locus_tag="NOA01_002456"
     CDS             3237..3776
                     /locus_tag="NOA01_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HCI0633612.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
BASE COUNT         1664 a          667 c          758 g         1372 t
ORIGIN      
        1 aaaaaaaact gtgttggtac tgggttctat ttctctagga atattaggtt ttagccccat
       61 gctattatcc gtaattggaa aaaacaattc caacagagaa cttaaattga attcaaatta
      121 ttacaacagt cctaacgaag aattgtttgt aaaagaaacg gataaataat aaaaaagact
      181 gctcttccga gcagccagtt aaaaaataat gagtgtttat tcttgaaaaa gcaacgcgct
      241 tataaagcgc taccaaaaat ttatcatatt ttatgaaaaa taacaatcca gtaaaaaatg
      301 actggtaaaa attcacaaag aaacggaaag aagcaactgc agaaaatcaa gccttaaaaa
      361 gagcttttta cctagaccaa ttaaaaatca caaagtgagc ccctgtatcg gttgatacaa
      421 gggctacttg gcatactgta cattatgtct actaaccttt aaaataacac gttttataaa
      481 ataaaccctc ttgacaatat ttaattttct gattattcta aatatataca gaaagcgctt
      541 tcctaggatt ttatatctaa ggaggaagta acaatgaaaa ataaaattat taaaattatt
      601 acttgtctat ttttgttcgg tatgattgtt ggagcaagta ctccgttaaa tctgtttcct
      661 tttagtgcta gaggtataaa agtggaagct gcaagttcac gatataatca taatcatcgt
      721 ggatttactt gctataaaat gaactattat gttactaata aaatgtgtag agatttgaaa
      781 aaaaattata ataagctaaa gactcctgct caaattgcat cttttattcc tattggtgga
      841 gttggaacat gggttattac aaatgctttt agtagtgctg ttgatgcaat gaatgtcttt
      901 gtaagagctg caaatcaagg aaaaggtgta caattaacat ataatgctca ctttagcaat
      961 acaacatcat atcaatacaa tgattacgct cgttatgtaa taaaataatc agaaagtgaa
     1021 actatctcat gaataaaaag attttagctg taattatttc aataatagtt ttaatcacta
     1081 tgttcttcat atttaggcta attttcaata taagcttaca aaaaagtatt ctttatctaa
     1141 ttcctatagc atttatctta gcaattttta gaagtattta cggaaataaa aactctaaat
     1201 agctaaaaat aaatcatact aaattttaca aaaagtggct agcattttct agccactttt
     1261 tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg agcttgcgcc cctttgacaa
     1321 gcggttattt gcaccaaata cggaggtatt tggctagcaa atatagagct tgccaaaccc
     1381 ttttagtgag ttttgtagaa agtaccttta aaggtacttt cacattacga acgatacgtc
     1441 cgtaggacgt actgaaaggg tgggacttat gagcaaatta atgcaaaatt tggctagcaa
     1501 aaaagctaaa aaatatatgc taataatcga gcgaaataaa cactttttca agtgagcgaa
     1561 tccgccatga caaagctgaa gcagtttatt tcagctttgt catggcgtgt gccgactttt
     1621 ttaaaagtct aggcacacga gcaaatatct ttatcaagct ctaatttgcg ttttaagacg
     1681 tttgaaacgc ctaagtatat aaatatataa gaataggttt aagagtgctt aaatcgaatt
     1741 ttagagggca tttagacaat tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg
     1801 ttttaagaac aagataaggg atttatacta ggactgtctt aaaatgtctt aaacgaacgc
     1861 tcagagcttc tcaaagggtt ccttatgaac ggcttttagc aaaggagccg gaggcgactg
     1921 cgcagtataa tgtgagagca gagcgatcac acctttttag cgaaccgtag ggagcaaggt
     1981 ttggaattgg cagttttgat aaattcgcga acctgtttag agaaaaaagg agtgattttt
     2041 taggcgggaa aattagcctc ccctcttttt atatctcttt ttatatctct ttttatatct
     2101 cttttatgtc ggctgtgtcc ttactctccc aagcaattca gagttttttc aagtaggttt
     2161 tctacgcttt tcaagtaggt tttctacgct tttcaagtag gttttctacg cttttcaagt
     2221 aggttttccg acattattga gtatttttat aaagtatgtt acttttattt catactttat
     2281 aaaagtagga gaagcttatg gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata
     2341 ttcctctgag aaaatttaat agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg
     2401 tacgtgaaaa aggtgtagat gagattattt tttcatttca cgatttgaaa aaactaagcg
     2461 gttatacaga acgtggggaa caatttataa aagatcttag tagcacgtat gacaaattat
     2521 taagtattaa tgcttggaat gatgatggaa atactttaac taaatttgtt gctttcacaa
     2581 aatatagcat tatacgagat aaacaacttg ttataatttc agttaatcca gaatttaaag
     2641 gactttttaa tcaattaaag aactggacaa gatttaatct agagcaattt actaatttgg
     2701 atagcacata ttcaaaaact atgtttcgat tattaaaaca atatcgaact caaggctgga
     2761 ctgaattttc caaagaaaaa ttttttgatt tactagatat tcctaaaagt tattggaata
     2821 gtccgtcaaa tattgacaaa tttatcttaa ggccaattaa agaagaactc actcccttat
     2881 ttaaaggatt aactattcgt aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt
     2941 atcgttttac ttggaaagct gaaattaacc atgctgacga tttttccaaa gggaaacaag
     3001 aagatttacg tataaaatta tttaatattg atcacaatgg agaattaaca cgagaagaaa
     3061 aatggcgcgc taaagatcga atactaaatc ttcccttagg cactcatgaa gctgatttta
     3121 aacaacaaca acaaactgaa gaaaaacagg cagaaaaacg agaactttca aacaaactca
     3181 aacaagattt actaggaaat ttacaaaact tattcgatta agcttaagga gtatttatgg
     3241 cagataaaac aatcaaagaa cttgcggaag aattaggagt tagtaaaaca gcaattaaca
     3301 aaaaagtttc tgacaaaaat cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga
     3361 ttaacgaaac cggtcaaaaa gctataaaat caatgttttt agtcgataac gcaaacttaa
     3421 atcgaaaact agttggcgaa aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt
     3481 ccgaattaaa actcctttct ttcctagaaa aacagttaaa tcgaaaagat atacagctac
     3541 aagaaaaaga taatcaaata aaacaaatgc aaaaattact cgaccagcaa caacaactca
     3601 ctctacaagc gaaccaacaa attgagaacc tacaagaaca gctccattta acttatacag
     3661 aagaaactcc aacaaccgaa tcaactgcct tttctgaaaa aaaagcagac aatacacaac
     3721 cacttattga aaaaaagtgg tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt
     3781 ttctagaagt tagttcggat agtagaggaa aaagttgtac tacctgatgg ggagatcact
     3841 caaattttta tgagaaagaa aaaatagttt acaactgtaa acaatcaatg tttatagttg
     3901 taaactatca aactatatgg tgacttattg cttaaaatag gttgaaatct cactcaattt
     3961 ttggtggaaa aacaaaataa attctaatcg attttgttga aaaagtcata aaaaaacaaa
     4021 tgtgatttta acaaaatttc aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat
     4081 tttgatcatc gtagggtcat tgtagaatca ttatgagatc atcgtgggat catcgaataa
     4141 agtatgggaa aggcttgcgg tgcttggtat taagggcatt ctccgcctcc caggttcttg
     4201 accattttct cttataagtg ggatttttac cacgttgaga gaaaatggtc aagaacctgg
     4261 gaggcggaga aaacatacca agcaccacaa gcaaaaaaag ttagagtaac ttccaaaaaa
     4321 gaaaaatttt ttagaacaac ttttgaaaat ttaattttga tttatatccg ttcaatttaa
     4381 acatttgata aactgaaggc tagtatttaa aatgtgtata caagtggtag cgttaaaaaa
     4441 agtggaggta taacctatgt t
//
LOCUS       DAIGOK010000156         4430 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.159, whole genome shotgun sequence.
ACCESSION   DAIGOK010000156 DAIGOK010000000
VERSION     DAIGOK010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.159"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(543..2048)
                     /locus_tag="NOA01_002457"
     CDS             complement(543..2048)
                     /locus_tag="NOA01_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HCI0633613.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="NOA01_002458"
     CDS             complement(2045..2731)
                     /locus_tag="NOA01_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCI0633614.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQFKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="NOA01_002459"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="NOA01_002459"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCI0633615.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          918 c          729 g         1544 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgaattggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat
//
LOCUS       DAIGOK010000157         4421 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.160, whole genome shotgun sequence.
ACCESSION   DAIGOK010000157 DAIGOK010000000
VERSION     DAIGOK010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.160"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            65..1216
                     /locus_tag="NOA01_002460"
     CDS             65..1216
                     /locus_tag="NOA01_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HCI0633616.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(1325..3364)
                     /locus_tag="NOA01_002461"
     CDS             complement(1325..3364)
                     /locus_tag="NOA01_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HCI0633617.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKIAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFKTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(3422..3979)
                     /locus_tag="NOA01_002462"
                     /pseudo
     CDS             complement(3422..3979)
                     /locus_tag="NOA01_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
BASE COUNT         1157 a          860 c          811 g         1593 t
ORIGIN      
        1 tattttttca ataatgtatg ttatgctaag ttcgggctat attttataca aaggaatgat
       61 gagaatggaa tttattggta ttttatgcct gatattgatt tcaacaacga ttggctcaca
      121 tatctcgcgg agattaggta tcccagcagt gattggacaa ttacttgttg gtgttttgct
      181 aggccaagct ggattgaatt gggtcaaacc tgatattcta gtccatgact tttcggagat
      241 tggtgtgatc ttattaatgt ttttagctgg tctggaaagt gatttatctc ttttgaaaaa
      301 atatttccga cctggtatgt tcgttgcttt gcttgggatc atcttcccgg ttgtttttgg
      361 ctggttgaca ggcaaagcat tccaagtttc gggaaatgaa gcgattttct ttgggattat
      421 tcttgcggca acttctgtca gtatttcagt agaagtatta aaagaactaa acgtagtgaa
      481 tacaaaagaa ggatcaacaa ttttgggtgc ttctgtggta gatgacattt tagtcgtttt
      541 agttctaagt ttcagcctat cttttctaac gggggaagct tctagcaatg cttctttacc
      601 gcttgttttg attgaacagc taatttattt tgtttttatt ttcttattag tcaaatggat
      661 tgcgcctttc ctactttcat ttgcagaaaa agtgtacgca aattcttcta ttatcatcgt
      721 atcgctagtt atttgtttag gaatgtctta cgtagcggat cttgttgggc ttagctctgt
      781 cattggtgct ttttttgctg ggatagctgt cagtcagaca aaggttaaag aagaagtata
      841 ccacaatgta gaggctttag gatatgcagt atttatacca gtattttttg tcagtgtagg
      901 attagaagtt gatttttctg actttagtga acaactactc tttatttcta tattgacgct
      961 tgtagcgatt ttgaccaaat tgcttggagg ttattttggt gctcagattg ccggtttttc
     1021 tcaacatagt tcattgatgg tcggtgctgg tatgatttcg cgtggagaaa tggctttgat
     1081 cattttacaa attggacagc aaagtcaatt gatcgaacca cagtactatt cacctttagt
     1141 tatcgtgatt ttattaagta cacttatttc tccacttgtt ctcaaatatt ttacaaaaaa
     1201 agtttatgaa gtgtgagtaa atcaaaaaga agttgtgaaa gagacatcgt gaaaaccgtt
     1261 tatttgaaaa aaggactgtg acgataacgc cacagtcctt ttactgtact taaaaagcaa
     1321 ttttttattg ataattttgg ttgaggtatt gcaatagttc cgatgctcgt ttggttgctg
     1381 aatcatcatc ttctttattt tccaacatgc tgaccatcat ataaccttgg ttatcaagat
     1441 tgaaagcaaa caagaaactg ttctcttgcc cctttacatc ctgtttttct ttgatttccg
     1501 ctgtaccagt tttcgctgcc aacggaatcc ctaaagcaga aagagaatgt gctgtaccat
     1561 ttacatcttg cacaacttct ctcagatctg gcacgatcgt ttgtactgct gtttcgctga
     1621 tgacattctt cttatctttt gtctctttat ctgcaatcaa tttaggatag acaagtgtgc
     1681 cattgttggt aaaaacagaa tacattgctg cttgctggat aggagtaatt agaagttcgc
     1741 cctgtccata tccagtatca gctagcaaga tatctgagtt aaagctatct tcattagaaa
     1801 tttgtgctgg attcatacta attggcaaat caaggtcttc accaaaaatg aatttatcta
     1861 agcctgtacg aaattttttc tcacccattt tcaacgtttc ttgtgccgca tatatattat
     1921 cggaatagat caaagcagtt tttaagtcta cttgtgatac atcactactg acacgcgttg
     1981 ttttaaaaga tccccaagaa ctgtcttttt gccatttaag cccgttgatc gtcaacactt
     2041 catttggatc gatagtgccg ttgtcgagac cgattgctgc tgtaatcatt ttaaacgtag
     2101 atccaggagc ataacctgtc gcaaatcggc tgatgaatgg atgttcagga ttttcttcat
     2161 aagctttgta atcttcttgt gagatcccgt ttgtcatttt gtttggatca tagcttggag
     2221 agctagcaag cgcaagaaga tcaccggttt ttggcgttgt cgcaacagtt gatgcagctt
     2281 ttcctcctag actgtcaaaa gctatttttt gtgccttcgc atcgattgtc aatttgatat
     2341 ctttgccatt ttgaacttca tgttcgatca gaactttttt ctctacaccg tctgcatctg
     2401 taatgcttaa tttcccacct gttgtcccac gaagatcctt atcaaaagcc atctccaaac
     2461 ctgagcgacc gatttttcca ttactgctta attctggatt cttatcaata tcttctgctg
     2521 tgatatctcc cacgtaacca atcagctgag cagctgcttc acccaaagga taatatctgc
     2581 cgtctacttc ttgaatggta gctccagctg gaagttctgg cgttgctcca tcaatgattt
     2641 tcaatgggac aaagtaatcg ggttgtaccc agctttgtga aatcgcctga ttgatagcat
     2701 cttctgttaa gtcgaaggag gaagcaatcg ctttgatatt ggctgttttt tcgcctccat
     2761 ctccaagttt acttggaacg actcctaatt gttttagttt gcctgttgtt gctaatggtt
     2821 caccatttcg atctataata ttccctcttg ctgcttcttg tgtggtcagg cttactttgt
     2881 catttccttc catttctgga aaaaccaagt tgggttgcca gttgatcgtg gtttgaccat
     2941 catttctgtc taatgttcct ttataagaaa gatctttcaa ttcacctaat gaggtattca
     3001 tctttgcttt atatgaaaaa ctgtaagtag aatcgtcttt tttatctact tttagattcg
     3061 atatctgaag tcctttgaca tcagcagcac cgtatatatt ctggtattta tctaagatct
     3121 ctttttcaga taatgcactt ttatttgccg ccttttttga tgtcatttct gcagctttgt
     3181 tataatctcc cttgttcaaa gcttggacaa attgctcaac tgtcttttct ccagcttcta
     3241 ctgcttgggt ttcttgatag tgctggtagt aaaaataacc gccacccgca gctgctatta
     3301 agatcactgc gccggcaata taagcgcctg ttcgattttt gccgtgcttg tcacttcttt
     3361 tcattttttc ctccttctct tgcttgtcat tcctttattt cactatttat acctgtttat
     3421 tctaacatat gtctttgagg aaatccatac cacaatccag aagcacactt tctggaaggt
     3481 tcgtttggat actaccaagt aatttcccaa ccgctttggg tagcttgaat attgaagtta
     3541 cgtctttcag ctgctgagag atagctttga tcacttgctg ctgccttctg atcctcccga
     3601 agtctccttc tttgtcttcc cgaaatcgag catactggag taaagtatta ccatccatca
     3661 cttgctctcc tttgtttata tatacgccat ctaaatctag agatttttct gcatcgatct
     3721 tgactccacc aggaaacagt tcattaacgc aatcgataaa atcttgaaat gtgatacttg
     3781 cataataggg agcttcaaat ttgaaatttt cctttaatgt ttgatttaat aaatcaactc
     3841 ccccataaga gtaagctgca tttatcttat tcattccgta acctggaata tcgacatatg
     3901 aatctctcat gatagagatc agttttgctt gttgtgtttt ctggttgtat tgtgcaacca
     3961 gaagtgaatc cgagcgacct ccaccaatcg atcttttttc accgcatctc ctcctcacac
     4021 agcaatccac ttgtcaggtg aggagcgagt ttgtctcatc aaataagtca gtcacaaaaa
     4081 tataaagaaa aggtcagaaa ccttttcgaa caagaattct gacctagcat catctcatcg
     4141 tccgagtttt agcactatac aacgtaaaga aaacattctt agctattgga aaaagcctta
     4201 atccaacagt ttctgtttta cccattggcg actttcgacg tttctgggca acagcctatg
     4261 tttgtatagg agcctcacct aacaaggtta ttactatatt ctatttacga gttcatgata
     4321 gacttttcct aatgattagg caatggaatt tgaaatgaaa tagtgaaatt taacaatctt
     4381 taatattttt tagaaaaaaa taacaaataa gttgaaagaa t
//
LOCUS       DAIGOK010000158         4389 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.161, whole genome shotgun sequence.
ACCESSION   DAIGOK010000158 DAIGOK010000000
VERSION     DAIGOK010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.161"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            60..1361
                     /locus_tag="NOA01_002463"
     CDS             60..1361
                     /locus_tag="NOA01_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCI0633618.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            1388..1648
                     /locus_tag="NOA01_002464"
     CDS             1388..1648
                     /locus_tag="NOA01_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCI0633619.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(2647..3882)
                     /locus_tag="NOA01_002465"
     CDS             complement(2647..3882)
                     /locus_tag="NOA01_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HCI0633620.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
BASE COUNT         1280 a          696 c          898 g         1515 t
ORIGIN      
        1 cattctttgc gttgtcatgc aatgaatggt tcatatttta ttgaaaagga gtggttttag
       61 tggaaaagct gatgaaatgg gtcgaagaga aacttgtccc accaatggca aaaattggta
      121 cacagcgtca tttattagca attcgtaatg gggttgtttc aacattgtca ttgattttga
      181 ttgggacatt ttttatggtc tttatcaatt taccgtttcc tggatggaat gaatttattg
      241 ccccttattc ggcaacgatt gttttaccct ttaggatgac gatgggattg atggctattt
      301 atgcaacttt tgttatgggt tcggatctag ctaaatcata tggattagat agtgtgacag
      361 gtggtatatt atctttagga acgttcttta tgttacaagt tcctgtaaac gttttaactc
      421 ctgaagaggc acctcttggc tgggtcttac ctatgagttc tttgggagca tctgggatgt
      481 ttgcggggat cttatctatg atttttgcag tggaagtata tcgtttcttt aaaaatcgaa
      541 atattactat caaaatgcca gaacaagtgc cacctgctgt tgcccgtagt tttgaggcat
      601 tgattccagg tgcagtcatt ttaacaatta cttggctgat tcgaagcgtc gcgggctttg
      661 atgtaaacgc tgccttatta tcgttattcg agcctttaac caatatttta gggaataatt
      721 tgcttggtgt tttactacca atgtttttga tccatttgtt gtggtcattt ggtatccatg
      781 gcatgagtat tgtaggggct gttgttcgtc caatgtggct gatcatgttg gatgagaatg
      841 caaaggcatt ggcagatggt acagctgcca cccaattacc ctatatcgct cctgaacagt
      901 tttatcaatg gacggtaaca attggtggtg caggagcaac gatcgtggta tctgttttgt
      961 ttatattctt ctgtaaatca aaattcttga aagaggttgg tcgtttttct attattcctg
     1021 gtatcttcaa tatcaatgag cccataattt ttggagctcc tatgatgttg aatccttaca
     1081 tgttcatccc gttcaactta gttcctttag ttttgactat tatcagttat tttgcagtca
     1141 aattagaaat ggtcaataga tttactgttt tgccaacatg gacattacca gctccaatcg
     1201 gcggttatct ttcagcagga aatgactggc gtgttgttat cttgatcatg atcaacacat
     1261 tgatagcatt tattatctat tatccatttg taaaagctta tgataaaaag atgctgtcag
     1321 atgaacaaag tcgtggagaa aacgaagtcc agactgtcta agtttggttt ttagaaggtg
     1381 ggaaacaatg tattggctga ttttattttt tgtttttatc tttttattaa cggctagtca
     1441 ccttatattg aatatgctag ctacatatca tattcaaatc aatcgatgga tttgggcact
     1501 ggcatcattt ttgatcgtga ttcttccaaa gatcattgta ccgcatatga atgttttgtt
     1561 ttcatggggg acatatgtac tttgtggaat ttttgctatc aattttatga tcgagcagca
     1621 tagatggttt gtcacatcga agctttaaat gttctaaaag tggttgtaac agaagtggtt
     1681 agcttcaaga aataagaaga aatttacgaa aattgcttct taaatttttg tgaaatgttg
     1741 gcttattttt cgtttggttg aacttattac cgcagatgca agcttttgac caccgtttat
     1801 tcggaatatg ggactgtgac aaggcttttg tcacagccct tttttacata aagattatta
     1861 ttgttttaac tgttttggca gaaagaagga ggaaaaaatg gaaattttat cagcaatgga
     1921 tttagaatta gtagatctac taccaaagtg atcaagtcaa atatagattt gggagcttac
     1981 aaccatatag taggggctgc agtatggtgt atgaatggta atatgtatga tgtgagtgtt
     2041 tattcaaatc atgaaacgtg tatagaaata attgtgctgg gaatagcaat cagtcatggt
     2101 gaacgtgact tttagtgtat cgttgctgac gacatagaac attctgacat gatctactca
     2161 atatgtgggg attgctggca gtttatatag gagtatgcat cggattgcga gatcattgtc
     2221 tcaacagaag agggaggttg attatcaaag ataaagtggt aaatcctaca tttgatttag
     2281 taattgttga ggggactagc ggcacaaatc tctacaaagc gatgcttcta gatctatatg
     2341 acacatttta agaaaatgta tttgcctaat tattatggtc tttcttttga agaaacgttg
     2401 agatgatcca accagagaaa ggtgtcaaag aatttgacta agaataaatg gcagacgcta
     2461 cctatgctca tttggatagc gtcagatctt ttattttaag gatgattttg tgttttttag
     2521 acaccgtaat ttaagtaact ctaacttttc aatccactat ttactttttt tgtattttca
     2581 ctgtctgggt ctcgaatagc gaaattactg atgagtctct attaaaaaag ctttttctac
     2641 ttgaaactat attgggtggt tgctcaggaa ggtttcgatt tcttgaattg cttgttgtgg
     2701 accatttatt ggaaaaacgg cttgcagcgt gcgtgcctgc tgacgaatct tgggtgaaga
     2761 aacttcgcgc agagcaatct gcagttttct ttcagtcact ggttttgtga gggcgattgc
     2821 gtttccgtat tttacacaat agtcaatatt acagctttgc tcaaattgca tccccacacc
     2881 gataaacggt ttgccggatt cacaggcagt ttggacggtc ccttccccgc cgtggataaa
     2941 agaaaaatca atgaattcat gcaaaagatg actagacaga tattctgtta ttaaaagatc
     3001 ttctgagtca gattgaatgt catcaagcag atagtcaata ggtgcaataa ccgctagatc
     3061 cggttgtttt tgtaagaaga acaaaatttt gcgtaacata cgcggattgc ctgaactacc
     3121 cattgcgaaa tagataatac gttttctttc ttgttgcttt cgctgtataa aagttagcac
     3181 ttcttcaggc aaggaagtac tgagcttagc aaaaattgga ccaacgattc gataattttc
     3241 cggcagctgt tcttcttcca aaaatagttt aggcgttgtg atgagattta gatcagcatc
     3301 catcatttgt aaacttgttt ttggtaaagg aagcatttct ttttgtgcag cgtgtttgaa
     3361 ggcattgggg atccatcggg tatttactat acgctttttt gtccagtgcc aaaggggatt
     3421 gcttaaaggg ccgaagaaac gtaactcgtg gggcatcagc gcatgctctg attgaaaaaa
     3481 aggttcactc aaagcatagg gtttaacata tactaaagga atctttttat tgcgggccga
     3541 taaaaaaatt gtcacattcg aacctgtcac aatcagtcga ggctcaaact tagcaatcag
     3601 actctgttca ctagtgatac gagaggcaac catttctttg gtaaaagggt ttttaagtgt
     3661 acgcaactgg tcaaatttta tgatttgttc ttcttgtttt ttcgtaagga tcggcgctag
     3721 taaatgatac tcaaagccac tttctgtgat caaaggagca aaagttttag aaaagccaaa
     3781 gaatacacag taataacggt ctttcatagc tttggcaacc tcaatcattc gtgttgtctc
     3841 agctaaattg aatactgcag gagcaaaaag aattgtcttc atgaaatcct ccttattgga
     3901 atgagtctat cccttcgttt cttactagta tactaaaaaa cacagcgttc gagtagccta
     3961 tgtatatttt gaatattaaa ctgtctctcg ctgttttcac aacttctaaa tttaaatttt
     4021 gtaatcaagt tgaaactcca attttcaaaa aaacacgcaa caattttttg atcgatttat
     4081 atgttgtgcc agataaatac gagaagttgt gtcattaaaa ataaacatga gaacataaaa
     4141 aaataaaata attatattaa ttgataggtg tgctgtcgtc attatacacg taagaaagaa
     4201 atagttatgt ttaaaaatat tcttagactt tctcgccatg acaattttca ctatgtgtta
     4261 taactgtaaa cgataattat tcagataaca caaaaagaaa tcaaggagca gatacgccag
     4321 ttgtttaatt tgaatattat tttaaaatta aaatttttat tctgttctaa acatttttgg
     4381 ttgtttaga
//
LOCUS       DAIGOK010000159         4386 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.162, whole genome shotgun sequence.
ACCESSION   DAIGOK010000159 DAIGOK010000000
VERSION     DAIGOK010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.162"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            2..74
                     /locus_tag="NOA01_002466"
     tRNA            2..74
                     /locus_tag="NOA01_002466"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="NOA01_002467"
     tRNA            78..150
                     /locus_tag="NOA01_002467"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="NOA01_002468"
     tRNA            155..225
                     /locus_tag="NOA01_002468"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="NOA01_002469"
     tRNA            238..311
                     /locus_tag="NOA01_002469"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="NOA01_002470"
     tRNA            323..394
                     /locus_tag="NOA01_002470"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /locus_tag="NOA01_002471"
     CDS             660..1853
                     /locus_tag="NOA01_002471"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCI0633621.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="NOA01_002472"
     CDS             1983..3455
                     /locus_tag="NOA01_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCI0633622.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="NOA01_002473"
     CDS             3538..4326
                     /locus_tag="NOA01_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCI0633623.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
BASE COUNT         1282 a          732 c         1021 g         1351 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 atagga
//
LOCUS       DAIGOK010000160         4381 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.163, whole genome shotgun sequence.
ACCESSION   DAIGOK010000160 DAIGOK010000000
VERSION     DAIGOK010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.163"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            54..935
                     /locus_tag="NOA01_002474"
     CDS             54..935
                     /locus_tag="NOA01_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0633624.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            948..2558
                     /locus_tag="NOA01_002475"
     CDS             948..2558
                     /locus_tag="NOA01_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HCI0633625.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(2635..3294)
                     /locus_tag="NOA01_002476"
     CDS             complement(2635..3294)
                     /locus_tag="NOA01_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCI0633626.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(3577..4242)
                     /locus_tag="NOA01_002477"
     CDS             complement(3577..4242)
                     /locus_tag="NOA01_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HCI0633627.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT         1173 a          856 c          868 g         1484 t
ORIGIN      
        1 aaactattta atcttgttta gtatatattt ttagaaaaag gagtggggtt tttatggcag
       61 aaatcgtcaa agtcacacat ttgaaaaaga gctttggtcg tttcgaagct ttaaaagatg
      121 tctctttcac cgtacattca ggtgaagtcg ttggatttat cggaccaaat ggttctggaa
      181 aatccacgac gattcggaca cttttaggga ttataaaaaa agacggcgga acggcaacga
      241 tttttgggca agatgtatgg gaagatagtt tatctatcca tgaacgtctt tcttatgttc
      301 caggcgatat ctctttgtgg ggaaatttga ctggaggagc gatcatcgat ctcttcatga
      361 agcttcatgg aaaagggagt gctcaaaaaa aagatgaact gattcaacgt ttctctcttg
      421 atcctaagaa aaaagcaaaa aactattcaa aaggaaatcg ccaaaaagtg ggcttgattg
      481 ctgctttggc tgttgattcg gatctctatt tatttgatga gcctacttct ggtttagatc
      541 ctttgatgga aacgattttc caagaagaag ttgaaaaatt gaaagaacag ggcaaatcca
      601 tcctactttc ttctcatatt ctcagtgaag tcgagcgact agcggataaa gtcgtgatca
      661 tacgcgaagg aacactcgat gaagaaggaa cactcgatga attgcgtcat ttgactcgtt
      721 cgaccatcac tatagaaaca gaagcagaga atagtcactt agcacaattg aacggtgtcc
      781 atgactttgt gcaaaaaggt cacctcgctc aatttgctgc tgataacgaa gctttcgatg
      841 tgattttagc tgaagcgtcc aaattaggtg tgaaaaaatt tgaatctatt ccaccgactc
      901 ttgaggattt attcatgaga cattatgaag gatgaggagg aagcgaaatg actgataaat
      961 tttcacgctg gtccaccctt ttttctcaaa accttagccg tgactggaaa aagatcctga
     1021 tatggctagt cggtgtcact gctttttctg gtggttttgt ccctgcgttt gaagaattga
     1081 cttccggaca aggattagct ggattatacg aaaccttgca aaacccggca atgattgcaa
     1141 tggtcggacc aactccagct tcgaatgctg ctgagtatac tttaggagcg atgtatgcaa
     1201 atgaaatgtt gcttttttgc agtttgtttg ctgctgtgat ctctgtttta cacgtggtga
     1261 gccaaacgag aaaaaaagaa gaacttggtc tgattgaact tattcgttcc tttcgagtcg
     1321 gacgtcaagc aaactcttta gctgtcatcg ctgaaagtat tctgatcaat ttgcttttag
     1381 caatatcgat cagctgtacg atgatttttt tccaagcgga ttcgatcact gcagaaggtg
     1441 cttggctttt tgggctatct gtaggtagta tggggatttt ggggagtgcc gttgcactgt
     1501 ttgctgcaca actgatgcca agttcagcca gttcgaatgg tctgtcttta gcgtttttag
     1561 gtggacttta ccttctacga gcaataacag atgtagctga ttcctccctt tcgatgtgga
     1621 atcctttagg ctggtcttat ttgacctatc catttacaac taacaactgg attcctttgc
     1681 tttatttgtt tttgttcagt ctaatcttga taatcagttc atttgtttta gaggaacatc
     1741 gagatttgaa caccggctat atccccgaac gtgaaggaaa accgtatgct aaacgttctc
     1801 ttttatcctt gcgtggttta ttattctatc taaataaagg gatgacaatc agttggctta
     1861 tcacttttgg tttgatgggg gcggcatacg gctctatcta cgggaatatg caacagtttt
     1921 tagaaagtaa cgacttaatg aaacagatgt ttatccaatc tggcgtctca attgaaaaaa
     1981 actttacaag cacgatcatg gtagtcttga tttgtctagc tgctatttta ccaattgcta
     2041 gcatcaatcg tttatttaca gaagaaaccc acggacgtat gaatcagctg ttcgcaacaa
     2101 aaatttctag gaaccaactt tattggacca atatgttact agctattcta tttgctatat
     2161 tggcaatcct gattagttct ggtgcactag ggctttctgc tttggcttcg atgaaggatg
     2221 cgaaagacat ggatctattg atgtttctta aagccgggtt caatttcctg cctgccgttt
     2281 tagtgtttat cggaattagt ggctttctat tcggatggct tcctcgaatc ggtaaacttg
     2341 cttatatcta cttagcttac tcctttgcac tcaattactt tggcggaatt ttggatttac
     2401 cagaatggtt ttccaaaaca gctgtcccta gctgggttcc acggatgcca aacgattctt
     2461 ttgatctttc cgtgttcctt acgttatcat gtatcagcct tgcactgttc gtccttggtt
     2521 ctattggtta taggcgaaga gacttactag aaggataata aaaaagcatg tacaatttca
     2581 tgctagcctc caaatctggg tcaaaaatcc aacatttgga ggtcattttt ttatttacaa
     2641 gtcttctaac tgctctttat caatgatcca gacttctcga ttctttcttt ttacataacc
     2701 attctcctct aataatttaa attttctaga taaagtttcg ggggtcgtac cgataaatgc
     2761 agcaagttct ttcattttca ttggcaggaa aaagtgatcc ttccctgtcg ctttagctaa
     2821 gtcaagcaga tagacagcga ctcgttcttc tactttttcc atcatcaaaa aagttgtctg
     2881 ctgctgtact tttgttaatt tctcaacgtt gattgctaaa agtttcagac taagggcggg
     2941 ataatgcagt aagagttccg agaaatcttt ttgtctcaat atgcaaattt ccgttttttc
     3001 tagtgcttcg ccaaataaag actcatttgg aacgcctagc aactggctct ctccctcata
     3061 tccgcccggc tctattactc gtaaaagctg ttcctttccg ctagcagata attgatacac
     3121 tttcatactg ccggaggcca cgatcactaa ttgaggttct ccattcggcg caagaatctg
     3181 ttctcctttt tccacatagt gatgtttgac aagtgcgtgt actttttctt gatcttgctg
     3241 tactaagtga ttgaacaaag gaacaaatga aacacataaa tgatcatgac tcataatctc
     3301 tcctccttct aaacagctat ttctaataaa gaaacataac ttcccttatt ccaactgata
     3361 attttctttt atcatagcat aaacgtcaag cgaaccagat accagcaaat agagtggatt
     3421 tctttttgtc ccttgcttta ttttccttct tttgagacct taagtaattg attcctatca
     3481 attttttaaa tatttacttt ttgaataatc aaaaacaaac cttcactcgc aagccgagag
     3541 taaggtttgt ttttcgcatt gcttatttcg tgttgtctat tgaaattttt ccgcacctag
     3601 cttttgatat agtggtgcta gttcttgtaa ggtttcagcg gttatttgct caacgtgttc
     3661 gatttcttgc ggatagaaga atagtttacc catttgaaag gttcggtctc tcatcgcttt
     3721 tttcaactct tcttgtttct ttgtatcgga caaatagtca gacaaagcta agtggtcgtc
     3781 aagttcatgt tctggttgcg gccatatgta ataatccttg atatcgactg ttttgctcca
     3841 ctctttcagt atcgcaagca actggttata cgctgcaatg ctaccatatc gttcttcgct
     3901 tttataatgt tggtacatca aatagatttg gtattgtttt tgattcaaca aaactgctag
     3961 acaggcattc tcgtcttgac gagattcgct tcggtacgcg ctccaaaaat gactgcgtaa
     4021 attccagcca ttcgtccagc tttcgatttt aggtttgctc atgccgtaca tatcaggcaa
     4081 aagtgcagct gtctggagct gaatctcttt ccatttttgc cagtgttctt tatattcttt
     4141 cttgatttgt tcaatcttat ctgttggatg gctctctttg agctgtttga atgcgtagat
     4201 tggtcgatca aacaactgat aatctgattt tccaagttcc attgtttcgc ctctttccgt
     4261 tatcttttgt attctacctg aattttgcga tatggacaaa tagaggttgt gaaaaagctg
     4321 ttctgcatca agtcataaga acagccaaac aaaaaactgc gatgagcatc accgcagttt
     4381 t
//
LOCUS       DAIGOK010000161         4375 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.164, whole genome shotgun sequence.
ACCESSION   DAIGOK010000161 DAIGOK010000000
VERSION     DAIGOK010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.164"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(83..1051)
                     /locus_tag="NOA01_002478"
     CDS             complement(83..1051)
                     /locus_tag="NOA01_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCI0633628.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLNVQTLVQTIKLNWSIDRITIVG
                     KLNQNIFYHTQHDVLILDFEQLMRVNEGNGYLKAVGNNGWQLLDQHDENIAYIEISKW
                     QEGKGRIDFNPNKISRFLADSMKNFIHDLFLEPHFSRADVACDLIDVPDEFITQYRII
                     DPVSFKLIYGRSGKLETVYWGSRASERQIRLYNKKIEQEMKRKVVPSEINTWWRLEMQ
                     LRRGKATEWYAMVKESLDSFVSPHFLPTDIKPTDKVMIDGLLANHSNWAILSRNSKYK
                     YRNLLKRESQNDELTNHLRETFEESADDLKKELDTWLQGLDVTEEE"
     gene            complement(1500..2906)
                     /locus_tag="NOA01_002479"
     CDS             complement(1500..2906)
                     /locus_tag="NOA01_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HCI0633629.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQTNIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKLEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPNLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDVHEYKEIMSEISELRQTAVKERESVSDELRYISRQREE
                     NEREKALVASERVKIEEEKKNMLNVKKEMAVIFQKDIVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVATLEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQRKLERSEDEIEW"
     gene            complement(3127..3489)
                     /locus_tag="NOA01_002480"
     CDS             complement(3127..3489)
                     /locus_tag="NOA01_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633630.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENATMGKALRKR
                     FGFESFADFERWLSSISDDQQHEKTVDNEWLADEKSAPSERDYPDESDGLFGVITNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(3493..3831)
                     /locus_tag="NOA01_002481"
     CDS             complement(3493..3831)
                     /locus_tag="NOA01_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633631.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERIMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            complement(3810..4229)
                     /locus_tag="NOA01_002482"
     CDS             complement(3810..4229)
                     /locus_tag="NOA01_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633632.1"
                     /translation="MTDLPEKMTIKNLADELGISKNTVRNQLVNAGYDLARYKDNGVI
                     MLDKELIGVVRSIYRSKGGQLTGDLPVNDAGNDFPLEKENEFLREQLAEKDRHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(4222..4362)
                     /locus_tag="NOA01_002483"
     CDS             complement(4222..4362)
                     /locus_tag="NOA01_002483"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633633.1"
                     /translation="MSGKLLDVTQYELILKNNKIDSPIIIPKHAVKYVYGVAESNGED
                     ND"
BASE COUNT         1251 a          978 c          603 g         1543 t
ORIGIN      
        1 aaaaaaacaa cgacaacgtt atcacctgtt aaattcacca accaattttt cttaccaaaa
       61 tgcttttgtt tttctgccat tactattcct cctctgtcac atcaagtccc tgtaaccaag
      121 tatccaattc tttttttaaa tcatctgctg attcttcaaa tgtttctcgt aaatgattcg
      181 tcaactcatc attttgactt tctcgcttta ataaatttcg atatttatat ttcgagtttc
      241 ttgacaaaat cgcccaatta gaatgattag ctaacaatcc atcaatcata actttatccg
      301 taggttttat atcagttggt aaaaaatgtg gactcacaaa gctatccaaa ctttctttca
      361 ccatagcata ccattccgta gctttaccac gtcttaactg catttctaac cgccaccaag
      421 tattaatttc tgaaggcacc acctttcttt tcatttcttg ctctattttt ttattgtaca
      481 aacgaatttg ccgctcgctt gcccgactac cccaatacac cgtttccagt tttcctgagc
      541 gtccataaat taatttaaat gaaactggat caataattcg atattgcgtg ataaactcat
      601 caggcacatc aataagatcg caggcaacat ccgctcgtga aaaatggggc tctaaaaata
      661 aatcatgaat aaaatttttc atggaatccg ctaaaaatcg actgatttta ttaggattaa
      721 aatcaatccg cccttttcct tcttgccatt tagaaatttc aatataggca atattttcgt
      781 catgttggtc taatagctgc caaccattat ttccaaccgc ttttaaatag ccattgcctt
      841 catttacccg cataagctgt tcaaaatcta atattaatac atcatgttga gtatgataaa
      901 aaatattttg attcaacttt ccaacaatgg taattcggtc aatcgaccaa tttaatttta
      961 tagtttgtac caaagtttga acatttaacc gcctgttaga gggggctgtt aatttttccc
     1021 actcatttat gatcgtgcct tttttactca aattttgttc ctcctcaaag aaaaagagac
     1081 ttctggaaag aattttcaaa agttcacgta actcttaaaa attcattacc aaaaaatctc
     1141 tagtcttgaa gaaatctaaa aatattcgta taatgattaa gaagttacgg agctagtgcg
     1201 attagtttcc gtaaaggtcg agaacgtgtc cagcgttgct cgactttatt tttttgctct
     1261 tttttcagtt tatttatttg ataactcaat aatagcactt ctctttagct atgcaaatat
     1321 ccaaaaaata aaaccgcacc ctttgaaaag ggcgccccca aacttcgcta acgctaggtt
     1381 ggccaaaaaa tggacttgcc attttttagc aaatgtcagc ttcagggatt agtggtttag
     1441 ccgtcaactc cttatcctcg gcacaaaccg cctccggtac cgttgactgc acatcgtaac
     1501 taccactcga tctcatcttc agaacgctct aatttacgct gtgagacgtt ttgacgataa
     1561 aataaggttt tctcctgctc taacacttct aattcctcag aaagccccat atccttttct
     1621 gcaagaccaa aacggataga caaacgagtg atcgattttt gaaatgcgtt ttttaatcgc
     1681 tctaaacgct ggcgtaaaga gcgattttct tctttcagct ttttattttc cacttgtaaa
     1741 tctgctaatt gttttctacc agacaaatga cgctcttctt ccaatgtagc tacatactca
     1801 tttaattctg tattttgctc aagcatctgt aaatattgct cactcatgtt atcagcattt
     1861 ctcttggcat ttgttgctct cgtagctcgt tgatacaaat caacaaaatc attcccagat
     1921 aaatgatacg aaccatcagg aagcctcttg aaactttcta aaggaacaat atctttttgg
     1981 aaaatgaccg ccatctcttt ttttacattc agcatatttt tcttctcttc ttcaatcttc
     2041 actctctcag aagccaccag agccttttct cgctcatttt cttctctttg cctagaaata
     2101 tacctcaact catcagaaac gctctcacgc tcttttacgg ccgtttgacg caattctgaa
     2161 atctcgctca tgatttcttt atattcgtga acatctttga ttttgttagt accgactaac
     2221 tcacgctccc aaccaaaatc tgccatcaaa cgttcaattg actcaacttc ctgattccta
     2281 aaattattcc aaatcactcg accattctct ttatctgctc gtaaaccctg ctgttgcaag
     2341 gctttgttaa aacttggttg cttttgaaca ccccgtttat acccttcagc aacaggaaca
     2401 atattcaaat gcaggtgcgg acttacttcg tcattatgaa taacagcatt gtagacggat
     2461 aaattaggat tccgttcttt aaattctttg aaatattcaa ctaaaatttt gttggcaatc
     2521 actcgatttt gttctgcttg ttgttccgat atgccagtag aagaaccatc atcacgagta
     2581 gtacgaaaat catcaagagt accaatttgc acgataaatt ctcgctgtgg ttccaatttt
     2641 ttatccttgc gaacctttgc taaatagtcc tcgatttttc tatcggctct tttctgcttt
     2701 gcattatatt tttctacggc ttcaccgaat aattcttcat atttttcacg aatatttgtt
     2761 tgttctaaat agatattttc atccgatttt gtgaaatcaa tatgcttatg ccagtcattg
     2821 attttttggc tatcagttaa ctcacgatta ttatgagaaa tgcttgtatt ctttgttccc
     2881 tttttgaacg aaatactgaa ccccaagtta aaaccctcct tcggctgtcg ctaataaaaa
     2941 gagtgtaaac ctttctgtta ctctttgtca aagagtaaca ttttcctaat tgtgacatgg
     3001 ctgtcacaat tccaaatgct ctccgagggt attcgctgtc acaagtgaca acgaaaaaag
     3061 gagccgaaag cgtgtcacgc tctcaactcc ttttacgctt tattttgctt cacgcaaaat
     3121 aatcgcttat cgtaaattca cgtcattaaa tattgacgta aatttactct cactcatctg
     3181 acttttctta ttcgttatca ctccgaataa gccgtcagat tcatcgggat aatctcgttc
     3241 gcttggcgct gatttttcat cagcaagcca ctcattatcc actgtttttt cgtgttgctg
     3301 atcgtcagaa attgacgata accaccgctc aaaatcagca aaagattcaa acccaaaacg
     3361 cttgcgaaga gccttgccca ttgtagcatt ttctttctca atcaagcgtt ttttctgttg
     3421 acgaagtttc gcttgttctt tttttaattt ttcttcttgc gctttaatat ctgctaaagt
     3481 cattcccata aactacatct ccttttcttt ggaattaata acggaaattt ggtttaactg
     3541 cataacatga ctcattaatt tcaaccaaaa ttctttttct gattcatcag ttgtttgcgc
     3601 taaatgctta tgaacaaatt cattagtcaa aactgaagat tttgctaaat cagcattgat
     3661 aaccgagttg agaacatcca taattcgttc aagcatttca ctataattat cttcatccgg
     3721 caaattcaaa taatcagcta accgttcata catcaattca attctatcga ctgtttcttc
     3781 tctcaaatca attttatcca tgtcacacct cacttccaga attgccacca ttttcgattt
     3841 tttaattctt gattttctag taacaactga tcgttttttt gttgctgaag caaagtcaat
     3901 ttttgttggt cacgaatcat ttccgttaat tcagaaatat gcctatcttt ttccgctaat
     3961 tgttcacgca aaaattcatt ttctttttct aacgggaaat cattgcctgc atcattgacc
     4021 ggtaaatcac cagtcaattg accacctttt gaccgataaa ttgaccgcac aacaccaatc
     4081 aattccttgt ctaacatgat aaccccatta tctttatacc gagcaaggtc ataacctgca
     4141 ttgaccagtt gattgcgcac tgtattttta gatataccca actcatcagc aaggtttttt
     4201 atggtcattt tttctggcaa atcagtcatt atcttctccg ttactttcag ctaccccata
     4261 aacatattta acagcatgct tagggataat gattggggaa tcaattttat tatttttcaa
     4321 aatcaactca tactgcgtta catcaagtaa cttaccggac atttttttgc tatcc
//
LOCUS       DAIGOK010000162         4338 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.165, whole genome shotgun sequence.
ACCESSION   DAIGOK010000162 DAIGOK010000000
VERSION     DAIGOK010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.165"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(<1..665)
                     /locus_tag="NOA01_002484"
     CDS             complement(<1..665)
                     /locus_tag="NOA01_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HCI0633634.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENE"
     gene            780..2492
                     /locus_tag="NOA01_002485"
     CDS             780..2492
                     /locus_tag="NOA01_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0633635.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            2495..4267
                     /locus_tag="NOA01_002486"
     CDS             2495..4267
                     /locus_tag="NOA01_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0633636.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         1341 a          753 c          923 g         1321 t
ORIGIN      
        1 tgctcgtttt ctcttctttt attttgttgg cataagcctt caatataggt ttgtgcactc
       61 gttagaaaga gtgctttttc attcaattct gcatgggact gcatactttc tttaaatccc
      121 tctaattgat tttcaactac ataattacgc ataaatagaa ctgtttcttt caaaaattca
      181 tttccgtcca taattttttg tcgaagctgc tgttcatgct gctgatccat taactgtcca
      241 atcaataaat tgtggagaga ggcaatggcc tgatgacttt gggtgactga aaatccttca
      301 cgaacaagta attggatcat ttgctccaaa tgttctaatc gactcggaaa agcgggcaca
      361 gtattgattt ctaattctgc cccacaaggg aaacgtgtat aaaggtcata gtaattttcc
      421 atatactgca gcaactgttc attccaaggc aaagttggat ctggaagcgc taattcttct
      481 tccattgttt cagccatttt ttgcaagagc tgttgtttgt ttttgaaata ccaatataaa
      541 gcaggtgctt gaacatgaat tctgtttgcg atgtttcgca tggaaagctg cgaaagagct
      601 tgtgtttcag ccaggatatc aaaggcggcc tgaatgatga ttgcttgaga aagctttatt
      661 tccattttat caacctcgtg tattgcatta tctttttaaa catggtaaat tgttagtcgg
      721 tctaatttta cagtgttaaa ttagtcctgt caatttaaac aaaggggtgt aatcaacgaa
      781 tgaaattaat cttacgacat gcgaaaaaat ataaagtagc tgtttttatt tccttgctgt
      841 ccgtagcagt catggttgca gcagcattat ggcaacctaa gttactacag caagtattgg
      901 aagctattat tacagaagat aatgatgaaa tgaaaacaat tggtatttac ctgatcagtc
      961 tggctttgct aggattagcc gcaggtgtaa cgaacacgat tttctctgca aaagtagctc
     1021 aaggcgtaag tgcagatatc cgagaagaag catttcgaaa gatccagaca ttttcatttg
     1081 gaaatatcga acagttctct gctggaaatc ttgttgttcg tctaacaaat gatatcacgc
     1141 aaatccaaaa tttagtgatg atttctttgc aatcactttt tcgaattcca ttcttattta
     1201 ttggtgcctt cattttagct atgatcacga tgccgcaatt atggtggatc atcgtagcgc
     1261 ttatcgttac ggtatttctg attacggcac tttcatttac aaggatgggc aagcacttca
     1321 tgatcattca aaaactgatt gatcgtgtga atagcattgc aaaagaaaac ttactaggga
     1381 ttcgtgtagt gaaatctttt gtacaagaag aaaaccaatt gaagtcattc agcaaagtaa
     1441 gtgaggactt aacaaaacac aacatcatag tcggtacatt attttctgtg atgatcccgt
     1501 ccttcatgct ggctgccaat ttggctgtgg ttggggcgat cttttttgtg agcgatcttg
     1561 ttaaagatga tcctacgtta attggtggaa ttgcttcatt catgaactac ttgatgcaga
     1621 ttatgatggc aatcattatt ggcggaatga tgatgatgat gacttcaaga gcagccgtat
     1681 cgctgaagcg gattgcggaa attttagatg ctgaaccaga tcttacttat ttagacgttc
     1741 cagaacaaga gttgacgggt tctgtcactt ttgaccatgt ttcttttcgt tatcctggtg
     1801 aggatacaga tacgcttaaa gatatcagct tttctatcaa acctggagaa atggttggta
     1861 tcgtcggggc gactggtgcc gggaagtcaa cactagcaca attgatccct cgtctatttg
     1921 atccgacaga aggcaaagtt gaagtaggtg gtgtagatct tcgtcaagta aatgaaaaga
     1981 gtttaagaca aacggtttcc tttgttttgc aaaaagcaat tttattctca ggaacgattg
     2041 ctcaaaactt gcgccaaggg aaaaagaatg caacagaaaa agagatggaa catgcttctt
     2101 ctattgctca agcaaaagaa ttcatcgaaa agttatctga tcgttacgaa gcaccaatag
     2161 aagaacgtag cagtaacttt tctggtggtc aaaagcaacg tttatccatt actcgtggcg
     2221 ttatcggtaa tccaaagatc ttgatcctag atgacagtac gagtgcactg gatgctcgct
     2281 ctgaaaagct ggtccgagaa gcactcgatc gagatttgaa gggaacaacg acgattgtca
     2341 tcgcacaaaa aattgcttct gttgtcaaag cagaccgcat cttagtattg gatgaaggca
     2401 gattagtcgg cgaaggaacc catgaggaat tggtagctgg aaaccgtgtc tatcaggaaa
     2461 tctttgaaac tcaaaaagga acggaggagt aagtatgact gatctaataa aagcaagtaa
     2521 atttttctat cactatttga agcggtacaa actctccttt ttctttatct ttatcacagt
     2581 gatcattgct acctatctac aagtaaaggc acctcaatac atcggggaag cttttcaaga
     2641 attagctaat tacgtaggag gcttgatgca aggcgtagac gataaaagca aatttgtttc
     2701 tgtaatatgg aaactcttgc tgttttatgt actagctagt gcagccaact ttatctatag
     2761 tattttgttc acacaagtag tagggaagtc aacgaaccga atgcgtatcg gtctattcaa
     2821 caaattggaa aaattgacga tccgtttctt tgattctcat caagacggtg aaattttaag
     2881 tcgttttacc agtgacttag ataatattca aaatagtttg aaccaagctt tgcttcaagt
     2941 catcacgaat gctgttttac ttgtcgggat cttgatcatg atgttccgcc aaaatgtaga
     3001 gttagcttgg gctacaattg cttccactcc agtagccgtt ttgatagctg tcttgattat
     3061 ccgcaaagca cggaaatatg tggatcttca gcaagatgaa gtggggaaac taaatggtta
     3121 tatggatgaa aaaatcagcg gacaacgtgt catcatcaca aatggcttac aagaagaaac
     3181 gattgaagga ttcttagagc ataatcaaag tgttcgcgaa gcaaccttca aaggacaagc
     3241 ttattctggt ctactatttc cgatgatgca aggaatgtca ctggtcaata cagcgatagt
     3301 tatctttttc ggcggctggt tagcattaaa tggtgatttg gaacgttcgg tggcattagg
     3361 cttagttgtg acattcgtcc agtattcaca acaatattac caaccactga tgcagatttc
     3421 atctggatac agcatgatcc aattagcgat tacaggggca agacgattga atgaaatatt
     3481 tgatgaaaaa gatgaagtca aaccagaaaa cggtaaagca tttgatggtg ttgaaaaagg
     3541 tcttgcgttg cagcacgtag attttggtta ttctccagaa aaactagtct tgaaagatgt
     3601 ctcgatcaat gtggacaaag gagaaatggt tgctcttgtt ggtccaacag gatcggggaa
     3661 aacgacgatc atgaatctgc taaaccgttt ttacgatgtc aatgatggtt cagtagtcat
     3721 cgatggaata gatatccgag attatgatct tgatgcatta cgtagacatg taggaatcgt
     3781 tttgcaagaa tctgtattgt tctccggaac gatcaaagac aatatcacat tcggcaaacc
     3841 ggacgcttca gatgaagaag tcatcaatgc agcaaaacaa gcgaatattc atgacttcat
     3901 catgacgtta gaaaaaggtt atgatacaga gatatccgaa gaaaataact tattcagtac
     3961 aggtcaaaag cagttgatca gtattgctcg aacgatcatc acaaatccat ctttattgat
     4021 tttggatgaa gcaacaagta atgtagacac ggtaacagaa gcaaggatcc aaaaagcaat
     4081 ggatgaagca atcaaaggaa gaaccagctt tgtgattgct caccggctga aaacaatctt
     4141 gaatgcagat agaatcgttg tcttgcgtga tggggaagtc attgaagaag gcagtcatca
     4201 tgagttatta gcagaaaatg gattttatgc ggaactttat cataatcaat ttgtatttga
     4261 aagttaaatt aaaagaacct cgttataacg gggttctttt tatatatttt atgttgtttg
     4321 tttttataag gggcagaa
//
LOCUS       DAIGOK010000163         4221 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.166, whole genome shotgun sequence.
ACCESSION   DAIGOK010000163 DAIGOK010000000
VERSION     DAIGOK010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.166"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(<1..2650)
                     /locus_tag="NOA01_002487"
     CDS             complement(<1..2650)
                     /locus_tag="NOA01_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633637.1"
                     /translation="MKIIGKKIANRKINWLLISFLILIAMIIFTSNVAEGSIFNWFDD
                     TKSQTEFLTNPLYQNYLKKYNGNFYSQFGMWIGWSVIKSMFAVVDGIQNMIPEVLDLF
                     SFVESAGLNKVYQSVLSTIVVGLMILSLVIVGYKMIIGKGTVDLKSVGMNVVMSISLI
                     LLMPTLIHSGIEFSKTFYNDSTTITDSDEGVAWSLIEQGVTDLAYINKTDQYSMIDKT
                     NDRNNLTKENFWKTDLTQVLTDNVLDKMEKENPIADNLRYELVENSDNEFVATKFSDN
                     FLSTFVDGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRVLGVLV
                     FATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFVNYLNAGEGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRSKGGKN
                     SGGNDQAEGEERKPERNFGQSLSQKANKTGKTLGYARERGLSGLASDGATMASERATK
                     PFKSMRDIANDTKNKFKEGLDDGTVSAINKNSKPIVAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNTNHASAIQQKVKQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEIMNREEMLRKRVEGRTGANMDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQDRRSVDVRENLQNTTSSQSGNRIVDVRENVQSSASNQSRTVDV
                     RENLQNTTSSQSGNRTVNVRENVLSSGTSQPRTVDVRENLQNTTSSQSGNRTVDVREN
                     VQSSTSSQGFKTVDVRENVQKDSGEMKEKAQKINIVEDRKSAKKFDSNHETVIIDSEI
                     RENDKGSKSRRRFTYEDNELFRDTLND"
     gene            complement(2875..3744)
                     /locus_tag="NOA01_002488"
     CDS             complement(2875..3744)
                     /locus_tag="NOA01_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaD"
                     /protein_id="HCI0633638.1"
                     /translation="MAEKRMLSKTFLNNASFMKMSDSAKLLYVYLNTNADDDGIVEAY
                     PYVKMLGTPDDDVKILFTKGFVFFLNEDLLAFLPHWLQNQNIRIDRYKPSIYRFKLLN
                     LKPEFFSKLKPIKLDVILKSGQVEPLRVDLMEKWYPVGIPSINHLEVQSSQDQISSDK
                     SSLVKNSIDKINQVDTIINEEIINEKGIQLIHKLGDRFTKSQREKFVQLIVDMKNSTE
                     REFKIRIDGPHYYEQTNKMIIRLFNTINEKLNLGKELNEMKYVNTVSRNHWKEIALEE
                     QDNRMYIIDALSK"
     gene            complement(3898..4200)
                     /locus_tag="NOA01_002489"
     CDS             complement(3898..4200)
                     /locus_tag="NOA01_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCI0633639.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGESFERK"
BASE COUNT         1189 a          866 c          562 g         1604 t
ORIGIN      
        1 gatcattaag tgtatctctg aataactcat tgtcttcata agtgaatctt cgtcttgatt
       61 ttgatccctt atcattttca cggatttcac tatcaataat cactgtttca tgatttgaat
      121 caaatttttt cgctgacttt ctgtcttcaa caatattgat tttttgtgct ttttctttca
      181 tttctccact atctttttga acgttctccc gaacatctac tgttttgaat ccttgactac
      241 ttgtagaact ttgtacattt tctcgtacgt ctactgttcg atttccactt tgactacttg
      301 tagtattttg caagttttct cgaacatcta ctgttctcgg ttgacttgtc ccactactta
      361 gtacattttc tcgtacattt actgttcggt ttccactttg actacttgta gtattctgca
      421 agttttctcg aacatctacc gttctcgatt gatttgacgc actactttgt acattttctc
      481 gtacatctac tattcgattt ccactttgac tacttgtagt attctgtaag ttttctcgaa
      541 catctactga gcgtctatct tgactacttg tagaattttg tagattttct ttgaccaatt
      601 tttctaatcc ggtatttgaa tttttagctt cttgaattct ttctttggtc aacgcttctt
      661 tttcgtccat attagcccct gtacgccctt ctacacgttt cctcaacatt tcctcacgat
      721 tcattatttc aggcgtgtat tttgcttcct gtcgcttctg attaattaat tcttcagcag
      781 acttagcttg accgtgcata gcatttttac gttcttcaat atcttgttta actttttgct
      841 gaattgcact tgcatgatta gtatttgatt cagcattatt tttcattgct tcacgcatac
      901 gttcagaact agacataatt ctatcagcgt tttcaacttt agcgccatct tttctatccg
      961 aaagacgcgt tggcatagaa tcagcatatt ttccagtttc atcttcttta tttttagcta
     1021 ctataggttt gctattttta ttaatagcgg acacggtacc atcatctaaa ccttctttaa
     1081 atttattctt ggtatcatta gctatatcac gcatactctt aaatggtttt gttgctcttt
     1141 cactagccat tgtagctcca tccgaagcta agcctgataa tccacgttca cgagcatacc
     1201 caagcgtttt ccccgtttta ttagcttttt gagaaagtga ttgaccaaag ttgcgttcag
     1261 gttttcgttc ttccccttcc gcttgatcgt ttccaccact atttttccca cctttagatc
     1321 ggttcatacc acctgttcct ttacgaaaca gcatagctcc cataccaaat gctgaagcaa
     1381 gctggccata gccttctttt aaaccaatat ccacgccaaa gtaacgcatg actgttccac
     1441 taccctttat tagaacgaac acggcacaga tataagcaat tgtttttata aaaatattcg
     1501 ttgagatacc ttcaccagcg tttagataat taacaaacat tgcaaacata gacaatccga
     1561 atccctgaaa accgacagtc aaataacact gtagaatatc tgacaaaact tgttttgatc
     1621 gttgtcctgt ttctaagtca gtagcaaata ccaaaactcc aagtacgcgt ttaaatgcta
     1681 actcgagtat tgctgtaatt ataatgaaag cactaaaaac atacgctact gctaaagcaa
     1741 tcaatccaaa tgaaattccc cacaagttcg cttgatagcg ataatatcct gatttgatac
     1801 catcaacaaa ggttgacaaa aaattatctg aaaatttagt agcaacaaat tcattatcag
     1861 aattttcaac taattcataa cgcagattat cagctattgg gttctctttt tccattttgt
     1921 cgagtacatt atctgtcaat acttgcgtaa gatccgtttt ccaaaaattt tcttttgtta
     1981 gattgttccg gtcattcgtt ttatcgatca tggaatattg atccgtttta ttgatataag
     2041 ctaaatcagt aaccccttgt tcaatcagcg accacgccac gccttcgtct gaatctgtaa
     2101 tcgtagttga atcattataa aaggttttag aaaactctat tccgctgtga atcaaagttg
     2161 gcattaaaag aatcaatgaa attgacatta caacattcat tccaacagat tttagatcaa
     2221 ccgttccttt acctataatc attttataac caacaatcac tagtgaaagt atcattaagc
     2281 caacaacaat ggtacttaaa actgattgat agactttgtt caatccagca ctttccacaa
     2341 agctaaacaa atctaaaacc tcgggaatca tattttgaat gccatcgaca acagcgaaca
     2401 ttgattttat aacagaccag cctatccaca taccgaactg actgtaaaaa tttccgttat
     2461 attttttaag ataattctga taaagtggat tagttaaaaa ctcagtttgg ctttttgtat
     2521 catcaaacca gttgaaaata ctgccttctg caacgttact cgtaaaaata atcatagcta
     2581 tgagaataag aaaactaatc aacaaccaat ttatttttct attggcaatc ttttttccaa
     2641 taattttcat tgttacctcc ctcagcccag tttctgaggc tctcagagag cttttgagca
     2701 attttttggt caatgatgat atgagttaca taactatcat tcactgctct taaatcgaga
     2761 acaatagtgt tgaagcagat acactcgatt aattcaagtg tatctgcgtt ttggtcaaga
     2821 cagcagattt gatctattag acgatttcca atcatattaa gagtcctttc ttgtttattt
     2881 acttagagca tctatgatat acattctgtt atcttgttct tctaaagcaa tttctttcca
     2941 gtgatttcga gataccgtgt ttacatactt catttcattc aattctttac ccaagtttaa
     3001 tttctcgtta atcgtattaa ataatctgat tatcatctta tttgtttgtt catagtaatg
     3061 tggcccatcg attcgaattt tgaactctct ttctgttgaa tttttcatat ctactattaa
     3121 ttgaacgaat ttttctcttt gacttttagt aaatctgtca cctagtttat gaatcagctg
     3181 tattcctttc tcgttaatta tctcttcatt gataatagta tcaacttgat taatcttatc
     3241 tatactattc ttaactaaac tagatttatc tgaactaatc tgatcttgac ttgactgaac
     3301 ctccaaatgg ttgatgctag gtataccaac tggataccat ttttccatta aatcgactct
     3361 tagcggttct acttggccag atttcaaaat cacatcaagt tttattggct ttaattttga
     3421 gaaaaattct ggttttaagt tcaataactt gaagcgataa atagatggtt tatacctatc
     3481 tattcgtata ttttggtttt gtaaccaatg cggtaggaat gctaacaaat cttcatttaa
     3541 aaaaaataca aaaccttttg taaaaagtat ctttacatca tcgtcaggag tcccgagcat
     3601 tttcacgtat ggatatgctt caacaattcc gtcatcgtct gcatttgtgt tcagatatac
     3661 atataataat tttgctgaat ctgacatctt cataaaactt gcattattta aaaatgtttt
     3721 ggacagcatt cgtttttctg ccatggattt cacacttctt tctatttgaa tggtaaaata
     3781 aattcaaata aagttttatc agaactttat tgttgggtgt ttcccttcgt ctacaaacta
     3841 tcgggagcac tcttttatat atttttttca attcttttca agtctaaatt tgttatttca
     3901 ttttctctca aaactttcac ctcttttcat tcgagcaaac ataaactcat taactgcctt
     3961 tttcgaataa aattttcttg cccctaattc aacaacgggc aatccctcat ttctcaccgc
     4021 attttttaaa aagttttcgc tcacatttaa gtattctgcc atttcttttt gatttagata
     4081 ttctctagtc atacccgcat tttcttgagc cttctcaatc gcttttgtag caatttgata
     4141 aaactgcttt agaaacattt cctcctgttc ttctgaaatc attaatttta ttgcttccac
     4201 aaaaacctct cctttttaat t
//
LOCUS       DAIGOK010000164         4171 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.167, whole genome shotgun sequence.
ACCESSION   DAIGOK010000164 DAIGOK010000000
VERSION     DAIGOK010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.167"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..470
                     /locus_tag="NOA01_002490"
     CDS             <1..470
                     /locus_tag="NOA01_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HCI0633640.1"
                     /translation="HSISPGIFGLLSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMG
                     GAYLPWFISSISMAILADIILSVFGYDRAIPQVVSWVLMQLGSAAGQWIPIWFFTDRF
                     RQDWIDRGQSAATMDAMIHYAVGIWGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            580..2334
                     /locus_tag="NOA01_002491"
     CDS             580..2334
                     /locus_tag="NOA01_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0633641.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQYLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHRVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            2294..4060
                     /locus_tag="NOA01_002492"
     CDS             2294..4060
                     /locus_tag="NOA01_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0633642.1"
                     /translation="MQMIGGLTMKNFYKKKFALTDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENAKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
BASE COUNT         1393 a          589 c          788 g         1401 t
ORIGIN      
        1 gtcacagcat atccccaggg atttttggat tattatctgg aatcatattt gtttatatta
       61 gctataacta ccctagaaaa ggaattttta ctatttacac aattgtttta ttgttatttt
      121 ttacgttaat gggcggggcg tacttacctt ggtttatcag ttcgattagt atggcgatct
      181 tagctgatat tattctatct gtatttggat atgatcgagc aattcctcaa gttgtgtctt
      241 gggttctaat gcaactaggc agtgctgccg gacagtggat tcccatatgg ttctttacag
      301 atcgttttcg tcaagactgg atagatagag gacaatctgc tgcaacaatg gatgcaatga
      361 tccactacgc ggtgggtata tggggaatta tttcagtgtt agttgtagca agtttatcta
      421 tgattggtgt attgattgga agaaaagtct taaaaaaata caaaaaatag aacgtagttt
      481 accttctatc tgtttgtaca tctctaattt acgctattac ggaaagcatt aacagttgta
      541 aacgaataag agaagaaatt gaatgcgggg gaggaaaaaa tgaaagtata caaaaaatta
      601 tttagttatg tccctaaaat gaaatactac ggatggttag ccactttatt ttctgatttt
      661 tctgtcgtat taacagtcta tggatattac agtatttaca aatttcttca gtctctaatt
      721 attgtaggag atgaatattt agctaagagt tatgcggttc aaactgttgc ttggttaact
      781 ttaggagctt tattttatat tttttctggc ttattttctc atatccttgg gtttcgctta
      841 gagactaatt tgagaaaaaa gggaattagc ggtttaacag aagcaagttt tcgttttttt
      901 gatttaaatt cttcgggata tattcgaaag acaattgatg acaacgcggc taaaacacat
      961 atggcaattg ctcacttgat tccagatagt gctcaagcca tagtaacacc tgttttatca
     1021 gttgccttag gctttttcat taactttaaa attggattgg ttctaatcgc catgttagta
     1081 attggtgtat tcttgttaaa gaaaatgaca gagaatacaa attttattca aaaatatcaa
     1141 gaatcgttag atgtgttaag ctctgaaacg gtagaatata tccgcggaat tcaagttata
     1201 aagatatttg gtgctaaagt aacatcattt aaagccttga ataaagcaat taacgattat
     1261 gctaagtttg catataagta ttctttgagt gggaaaacac cttttgttct ttttcaatgg
     1321 ctattttttg gcatagtttg cattttgata gttcctataa ccttttttat aacttctctt
     1381 accactccca agatcttagc tgttgacttg ttgatgttgt tttttttaag tggaattatt
     1441 tttgtttctt tcatgagaat aatgtatgta agtatgcact tgtttgaagc aagatatgca
     1501 gtcgaaacac tagactccct ttatttagaa atgaagcaag accaactaaa atatgggaac
     1561 gagacacaga ttaaaaattg tgacattgca tttgaaaatg tcagcttttc atacaaagaa
     1621 gagtgtgttt tagaaaattt caacttagtt ttagaacaaa aaagaattta tgcattagta
     1681 ggaaaatcag gaagcggaaa gagtacgata gctaaactaa tttcaggttt ctacaatgtg
     1741 gataagggac agattaaaat tggtggtaag cctttaaatc actattcaaa agacacaatt
     1801 attaacacaa ttgcttttgt ctttcaagat acgaaactat tcaatcagag tatttacgat
     1861 aacgtttcta tagcaaatcc tcaagcgcca gcaagtaaag tatttgacgc gctaagatta
     1921 gccggttgcg aatcgatttt agaaaaattt cccgaaagag agcatacctt aattggtagt
     1981 aaaggagttt atctgtcagg aggagaaaaa caacgtatag ctattgccag agcaatttta
     2041 aaagatgcaa agattgttat tatggatgaa gccagtgcat ctattgatcc agataatgag
     2101 tatgaactac aaaaggcttt tcaatatttg atgaaagaaa aaacagttat tatgatagct
     2161 catcgattat ccagcattaa atctgtagat gaaattcttg tcttgtcaga aggaaaaata
     2221 gtagaacgtg gaaatcataa tgaattaatg gctaaacatc gagtgtataa acaattggtc
     2281 actaattatg agaatgcaaa tgattggagg cttgacaatg aaaaattttt ataaaaaaaa
     2341 atttgcttta actgatcagg gcgcagaagc tttaactaaa gcttctattt ctagtttttt
     2401 tgtatactgc attaatatgg taccagcatt cattataatg atgctgatag acgaattagt
     2461 tttagaaaat gcaaagcccc gttggcttta ttttgcagta tcgtttgtta ctttgctttt
     2521 catgtattgg ttgttagata gggagtatga aaacttatat aatagtactt ataaagaaag
     2581 tgcgcattta agagtgcaaa ttgcagacga tttgtcaaat ttaccattat cctatttttc
     2641 aaaacataat ttatcagatt tatctcaaac tatcatgtct gacgttgaag gtattgagca
     2701 tgcgatgagt catgcaatac ctaaagccgg cggtatggct ctgtttttcc cttttatttc
     2761 agtgatgctt ttggttggta atgtcaaaat gggattagct gttattttgc caacgttatt
     2821 tagttttgtc ttaatcttgt tatcaaagaa atcccaaacg aaagccaata ctaaatatta
     2881 cgatactttg agagaaaact cggaagaatt tcaagaaact attgaattgc agcaagagat
     2941 taatagcttt aatctatcta aaaaagttca agacagactt ttcaaaaaaa tggaagagag
     3001 tgaaaggatt catttaaagg tagaattaag tactttttca gtcatggcct tatcctctat
     3061 tttctcatat gttagtttag cagtagtcat tctagtaggt gttcacttac tgttaacggg
     3121 tgaagtgact atactctacg tcgttggtta cttactagcc gcaataaaaa taaaggattc
     3181 cttcgattct atgaaagaag cagtgcttga aatattttat ttagctccta aaatacagcg
     3241 aattagagct atgaaggaaa ccagtatcca agagggatca gatagtcctc tgaaatcttt
     3301 tgacattgag ttgcgagatg tctctttctc atatgataac aataccccaa ttttagatca
     3361 tatctctttt acggctaaac aaggagaagt gactgcgtta gtaggtgcta gtggttccgg
     3421 gaaaactagt atcttgaaat tagtgtctag actgtatgat tatgatgaag gctgtatttt
     3481 aattgatggg tatgatatta aacgtgtatc tccagcttct ttgtttagca aaatcgctat
     3541 tgtttttcaa gaggtaactt tgtttaatac aagtattttg gaaaatatac gtattggaaa
     3601 tagtcaagcg tcagatgagg aagtgaaaaa agcagctcgt ttagctaact gcgaagactt
     3661 tattgaaaaa ctacctgacg gttatcatac attggtcgga gaaaacggaa gttcactttc
     3721 tggaggagag cgacaacgcc tatccatagc acgggctttt ttgaaaaatg cgccaatttt
     3781 aattttagat gaaattactg ccagtttaga tgcggaaaat gaaaaacgta ttcaggaaag
     3841 tttaaatcgt ttaatacaag ataagacagt actaattata tcgcatcgct tgaagtctat
     3901 tgaaaaggta aataaaattg tagtgatgga tcaaggaaaa ctagttgatc aaggtactca
     3961 taatgagttg tatcgaaggt cagaaatata caaaaatctc ataaagaaaa ctaaactatc
     4021 tgagaaattc gtctatgaaa aagaagctca atcacgatga tgaaataggc tttaaattaa
     4081 cctacaattt ttgggatata gttataattc tttttcaaaa agaataattt tattaaacag
     4141 catctaattt tgaatacacg aaagaatcag t
//
LOCUS       DAIGOK010000165         4121 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.168, whole genome shotgun sequence.
ACCESSION   DAIGOK010000165 DAIGOK010000000
VERSION     DAIGOK010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4121
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.168"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(45..1022)
                     /locus_tag="NOA01_002493"
     CDS             complement(45..1022)
                     /locus_tag="NOA01_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCI0633643.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            complement(1025..2134)
                     /gene="pdhA"
                     /locus_tag="NOA01_002494"
     CDS             complement(1025..2134)
                     /gene="pdhA"
                     /locus_tag="NOA01_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HCI0633644.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            complement(2595..4019)
                     /locus_tag="NOA01_002495"
     CDS             complement(2595..4019)
                     /locus_tag="NOA01_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCI0633645.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
BASE COUNT         1132 a          874 c          722 g         1393 t
ORIGIN      
        1 aatttaaatt gataagccat gactttgttt cgtcccttct tttcttaaaa ttccgcgatt
       61 tcttttactt tgtcttcgat atcttttgca tttggcaacc agacattttc tgcttgtcca
      121 aatgggaaga ctgtatcagg tgcagaaact cgtccgattg gggcttctaa tgaaaggata
      181 gcgcgttctg agatctcaga aactacttgc gcggcaacgc ccgcttgtcg ttgtgcttct
      241 tggacaacaa ctacgcgacc agttttttcc acggattgaa tgatcgtttc tacatctaat
      301 ggcgcaactg tacgaagatc aatgatttca acagatatat tttcttttgc aagattgtct
      361 gctgctttga ttgcttcacg caccattgct ccgtatgtga taatagatac atctgttccc
      421 tcacgagtaa ctgctgcttt atccaaagga acttcgtaag cttcatccgg cacttcttct
      481 cggaatgagc gatatagttt catgtgttct aggaaaacaa ccggatcgtt acttctaata
      541 gaagaaatca atagtccttt tgcatcataa gggttggaag gaatcactac tcgaatacca
      601 ggagactgag cgatcaaacc ttctaaatta tccgaatgca attctggtgt atgaacaccg
      661 ccaccaaatg gcgaacgaat tgtgatgggt agatttctag tgccgctcat tcgatatctt
      721 gtacgagcca tttgagctac tacttcgtcc attgcttcga aaataaaacc aaagaattgg
      781 atttcaggaa ctggacgaaa tccttctaac gcaagaccga aactcaatcc tgcgattcca
      841 gattcagcta aaggagtatc gaatactcgg tcttctccga acttttcctg caatccttca
      901 gttgcccgga aaactcctcc gtttttccca acatcttcac caaaaacaac gacattctcg
      961 tcattttcta gttcaagtgc taaggcatct gtgattgctt ggatcattgt tttttgtgcc
     1021 atgattattt cgactccttc gcttcataaa ttgcaatttg ttctttgatt gattgtggag
     1081 atacttcgaa catgtttttc aagaaatctg aaaccttttg ttttggtact ttgtcggctt
     1141 cagcaatagc tgctttgatt tcttctttag ttgcttcgat gacttgttct tctttttctt
     1201 cagaccaaag tcctttttcc gtcaaatatt tacggaagcg gatcaatgga tcttttttct
     1261 gccaatcgtc atctgtgtct tttgtacggt agcgggttgg atcgtcacca gatagtgtat
     1321 gtggtccata acgataagtc agtgtttcga ttaggactgg tccatttcca ctgatcgccc
     1381 attcccgtgc cattttagat acagtatata ctgcaagcgg atccattcca tcgacttgaa
     1441 taccaggaat cccagctgcg acagcttttt gtgcaagtgt ttttgctgca gtttgttttt
     1501 cacgaggtgt agagatcgca tacccgttgt tttggatata aaatacggca tttgcatggt
     1561 aagcgccagc gaaattgatt gcttcgtaaa aatcaccttg tgatgaaccg ccatcaccag
     1621 tataagtgaa gacgacattt ttcttgtttc gtttcttcaa acctaatgca acaccggctg
     1681 cttgtacata ttgtgcaccg atgataattt gtggtggtag agctttcaat tcttccggat
     1741 aagaattacc tgcagcatgt cctcgagacc ataaaaaagc ttgagataaa ggtaaaccat
     1801 gttttactag ttgaggtaca tcccgataac ctggaagaag aacatcttct ttatcaaatg
     1861 cgtaagcact agccagctga cttgcctctt gtccagcagt tggtgcaaaa aagcctaggc
     1921 gtccttggcg gttcagcgct gttgaacgtt ggtctaatac acgagaccat accatatgag
     1981 tcattaattc aacgagttca tcatcggata aatcaggtac taaatctttg ttaactacgt
     2041 ttccatcttg atcaagtgct tgatatactg ggaaatctgc attgacttca tctagcagtg
     2101 cttggaagtc gatcggtgct tttttgtttg ccatttgtca cacattcctc tctcatctat
     2161 aagaaaatct cttcattctg cgattgaaac agtgaataaa actgtatcac tatttaaccg
     2221 tcttcattaa taaattatca tggatttatg agaaatacaa gctaaaagca caaattttca
     2281 cgatgaaagg aaattatgcc aatccgcata ttaacagcat tagtgaataa aatcacaata
     2341 aggaaagcgt tttatatttc atctgcattt tttctatttt ctaatcaaac ttacaaccat
     2401 ctgtttctat ttaaaaacgt tggtgttata cagttttgtt gcaaacattt attttattta
     2461 cacatcgtga acggacgtac aaataatggt tttttcttag tttctttata tcgtattctt
     2521 tttccgaaaa aaaagaaaca gcttgaaatt taaatccaag ctgttatatt ctttaatcta
     2581 tttactttgt gaaattattc tgttaaccac ttttctagaa ttttcatctt tgaataaaac
     2641 aacggtattt ggttttcatc cagcctactg taattgattc tgattccatt tatatcttgc
     2701 ccaaaaagta gacttggtgc aattccaaga cccatatcta ctactttttc tgctagttcg
     2761 gggtctagtt ttttcccatc ccaagtaatc caggcatagt aaccacctaa aattgaatga
     2821 agcttgaaaa acgattgtga atctccccaa gcgcttatca gtcggctttt ttctttcaac
     2881 ttttttctta catcttgcat tttcaaatca attgcttcat taaatacatt agccactgtg
     2941 atttgtgtaa ggatattcat cgaaaactcc atcatttctt gcgcttgagc tagttgcgag
     3001 ataaaagtct caggtcctat cacccaaccg attttcgttg tttttcctaa aaccctagat
     3061 aacgaaccga cgtataacac attatccgga tcaatcgttc gtataggaag gacttcttct
     3121 tggtttagaa atgaaagatc cgtaaataca tcgtcttcaa taattggaag ccgatactgt
     3181 cgacacaatt ctactagctt ttctcgtcgt ttttgactca tcacttttcc tgtagggttt
     3241 tgaaaattcg ggttgataag cactgctttc aatttatttt tatggatcga ttctttcagt
     3301 ttttctaagt cgatcccttc ttcatccatc ggtatcccaa ataaacgcgt tcctgaagct
     3361 ttgaaaagag gtaatcggta aaaaaaagaa ggtgactcta cagctatccc ctctccagct
     3421 gaaagaatcg tttgtaaaat caaaaagatt gcttgctgac cgccaccagt cacaagaagc
     3481 tggttggggg aaaatgccaa ttcaaattta ctttccaaat accgtttgat tttgttacgt
     3541 aacggagtat agcctaaaag atccatttct ttttcttccg ctaagatttc ttcaagagaa
     3601 taagaaggaa actcaaaatt cggcaaaagc tcatctggca tctcgctagc gtataaatca
     3661 agaaacgatg ttttttggat ctgctttgct tctcttattt tttgctcata ccagtccgtc
     3721 tgctcataac gaggagagat catttgccgc caatcgattc ttggaacagc gaatttcccc
     3781 cattcagtct gattaacaaa ccatccgctg ccacgtttgc tcgttagtac cccaagctcc
     3841 ctaagctctt ccaatgcatg gacaacggtg gaacgattca caccaaaaag ttgccctagt
     3901 tttcgttctg attcaagttg acttccagga ttgatccttc cgttttcgat ttctctcata
     3961 atctgttcca taatttgacg ataaatcggt ccttgttttt tctctacttt ttcccacata
     4021 agcttctctt ttctattgtc tattttttct tttgatttta ccgaatttac gagaaaaaag
     4081 aaaaactttt tttgcaaaac ataagggact gtgacaaaaa t
//
LOCUS       DAIGOK010000166         4080 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.169, whole genome shotgun sequence.
ACCESSION   DAIGOK010000166 DAIGOK010000000
VERSION     DAIGOK010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.169"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            56..1480
                     /locus_tag="NOA01_002496"
     CDS             56..1480
                     /locus_tag="NOA01_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCI0633646.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(1661..2434)
                     /locus_tag="NOA01_002497"
     CDS             complement(1661..2434)
                     /locus_tag="NOA01_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCI0633647.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2431..3336)
                     /locus_tag="NOA01_002498"
     CDS             complement(2431..3336)
                     /locus_tag="NOA01_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCI0633648.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(3490..3810)
                     /locus_tag="NOA01_002499"
     CDS             complement(3490..3810)
                     /locus_tag="NOA01_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HCI0633649.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
BASE COUNT         1227 a          751 c          818 g         1284 t
ORIGIN      
        1 aaaaaggttt gttatacttt ttgataaaag cggttttatt ttggaggatg cttacatgga
       61 tacagagcaa cagaaaaaag gaattggttt aattccactt gctgccttag taattggttc
      121 agcgattgga ggtggcgtat ttggtattat gacggacatc tccggatcgg ctggcggccc
      181 agctattttc agttgggtac tcgtaggaac agcaatgttg atgctctcct tgtcgatcaa
      241 taatatcaat aaaaaacgtc cagatttaaa aggcgggatc tttagttacg cagaagaagg
      301 ttttggccgt ttcgctggtt ttatcagcgg ttggggttac tggctcacat gctggttagg
      361 caacgtagct tttgcaacat tgttgatgag cgcattagga tatttcttcc cagtatttga
      421 aggtgggcaa aatctggcat cgatcgtgat gagtacagtc tttttatggg gatatgcatg
      481 gctagtgaat cggggagtcg aaaatgctag tttcgtcaat gctatcgtca cattctgtaa
      541 attagtgcca ttatttttat ttattgtagt agctatcagc acgttttcga tccaacaatt
      601 cattgataac ttttctagcg acattatatt gaatgcgaat ggacagccga tcccgttttc
      661 cagtcaagta ttgagttgca tcatggtgat gctttgggta tttgttggta ttgaaggagc
      721 ctctgtcgta ggttcacgag caaaaaaaca atcagatgtt gggaaagcaa cgattctagg
      781 gatattcggc ttgattatta tttacgtctt ggtttccatg cttccatatg ggacaatgac
      841 gatgaatcag ctgcaaacga tcaaggcgca gcctgctatg ggctatgtgt ttgaaatgat
      901 ggttggtaaa tggggagcag tcgtgatcaa cggcggtttg atcatttctt tagttggtgt
      961 ttggctctca tggacgatct taccgattga aacaatggga aacatggctg aagacggact
     1021 tttgcctaaa gcatggggaa aactcaacaa aaatggggca ccaacttatg ctatcgtatt
     1081 gacgacgtta tgtacgaatg tctttttact ttcattatta ttcactgaca gtgcttataa
     1141 ctttgcttac acacttggta cagcagctat tttcttcacg tggcttttct taggattgta
     1201 ccaaatgaaa ctttcttatc agcggaaaga atgggtccaa tttttagtag ggcttttagc
     1261 aagtgccttt caagtatggg ctatgctagt cgtagccttt aatgaagtaa tgcttgttct
     1321 tgttcttttc ttgccaggca tttattttta cctcaaagca cgtaaggagc aaaacgagcc
     1381 tgtacaacat atccacttag ataagatttt acttataggt gtgacgatgg tcggagtgat
     1441 tgcaatggtt ttatttgcca ctgggatgct tcacgtatag aagtataaaa gaagtataaa
     1501 gagaggttgt gagcctgtcg tttattcaaa aacatactag gaaaggagct gtgacacaaa
     1561 tgttgtcaca gctccttttt cagaataaac ggtgtttaag agacagccaa tcggcaataa
     1621 ggctgaacgt ctctttcaca gcctcttttt tgatgagtca tcatacattt tttcgcttaa
     1681 atacgagata tcctaatgct aagaacaaaa cgatgtaagc taaattaccg aatagcattt
     1741 gattagtaga aagatgagtc aattgcttca tgatctcttc gtttccaatt tggttttgca
     1801 gattcaacat actgatcgga ttccatttga tccactccca tttttggatg agtgcaataa
     1861 ggataccaga tatgattgac gaagcaaaat aaaagacgat tcctgctgag atggatgcgc
     1921 cggaactatt gataaagcaa gcaatcatca acactaaact taaaatcagc cataaaccga
     1981 tataactacc caaagtataa gtgaacaagc tttggatcag tgtttgacca gtagaaacct
     2041 tctccgtgaa ggaaatcgaa gggaaaagaa tcagtttcat caacacagtg acaataatag
     2101 ctaaaaggta taaataaaca gaataaataa ctaatgtgat ccatttgctt actaatattt
     2161 ctcctcgaga atactttcga taaaggagat ttttcaatgt accatattgt gcttccatcg
     2221 aaatgatcgt gcttgcagct gcaatcatga taaagacaat ccaagatgtg gcactaaaaa
     2281 gctgagcagt catttcaact ggatcaatga tcgtcgtgta ttttttggct agaagtgctg
     2341 ttcctatgag caaaacaact aatagaacag ataaaacagc agttgacttt ttcttgatca
     2401 atttaaataa ttcttgtttt acaagcaatg tcatttttct tcttccccct ttactgaatc
     2461 cagaatttcc aatagagaag attccaaatc attcgtcaga tgctggacgt ctgtcacatg
     2521 gatattttgt gcagacaata gctggatcac ggttcctaat aattcttcgt tttcttttaa
     2581 aagcttgatt ttatcaggat cttccaaagt atatccagct tctgtcaaga tcgttcgagc
     2641 agctagatca tttgttgtag agataacgat gaaatcttgc gctgtttggt tcaattcagc
     2701 cattgtacat tgtttgatga tctttccttg attgatcagg atcagctcat cgaccatttt
     2761 ttcaagctcg ctgagtaagt gactggagat caagaaagag gtgccctgag tagccaagtc
     2821 ggtaatgatt tttcgcacat ctcttgttgc ttgtggatcc aaaccgttca tcggctcatc
     2881 taaaataacg atagacggat ggttcagtaa ggcaagagca attcccaatt tttgtttcat
     2941 tcccaacgag taggatttcg cttttttgtg aatgtacttt tccatcttga gctgattcac
     3001 gatagtgtcg atagcagata catcgttggt ttcagagaat aacttcagat gatcatagcc
     3061 tgttaagaat ggatagatac ccgggtattc gatcaatgct cctacttgtt ccaacccttt
     3121 atgactagag ggagaaatct cattttcacc aatacagatt gttccatctg agtaatggat
     3181 cagtcctaaa atagctttca tgatcgttgt ttttccagcg ccattaggac ctactaatcc
     3241 aacgattttg ccaggggata agctaaaact gacatcgctg agggcttgtt tgtgaccaaa
     3301 gttcttggat aattcttgga ccgaaagact tttcaaaaat attcactcct tttctataga
     3361 tagtacaagc atacacctga tgagtggaga agtcgctagg tcattggact tatttcttat
     3421 tttttcataa aaaaattgaa tattaaataa aaaggcttat tttttctgat cagtacttat
     3481 tccaagtgtc tagtggccgc tagtaatctt gatgccaatg atactgatga gtagcatccc
     3541 cacaaagaac caagtggaaa gattcagctt atcatgaaac aagaccacac caattatgac
     3601 cgaaccaact gcgccaatcc ctgtccagat aggataagag agactcatcg gcaaactttt
     3661 gattgccttt gacaaaaagt aaaaactagc aatcatgcca atcactgtgt aaaaagaata
     3721 attgatttta gaaaaaccat cactccattt catcatcgtt gaccagaata cttccaaaat
     3781 tcctgctatg attaattcca tccatgccat acttacattc ctccttaact ataaaaaaat
     3841 acacaaaaag aggaaaatca tcccttcaaa gacctaatgg aatgatcatc ctctttatcc
     3901 tacccggtgg cagattgtat atctttttct gtagtatagc acacctatcc agatatcggc
     3961 aatctcatac tttttagtaa agtgtaccaa gcactgacgg atatatcgtt agcggaaaag
     4021 aagatgaatg ctttgcaatt tcatagaaag aagttgttat ttattaatca tttaaaaaaa
//
LOCUS       DAIGOK010000167         4073 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.170, whole genome shotgun sequence.
ACCESSION   DAIGOK010000167 DAIGOK010000000
VERSION     DAIGOK010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.170"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..679
                     /locus_tag="NOA01_002500"
     CDS             <1..679
                     /locus_tag="NOA01_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HCI0633650.1"
                     /translation="EIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDTIR
                     SEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIVYY
                     TKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMHIT
                     IYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLGLV
                     DNFNNIS"
     gene            696..1130
                     /locus_tag="NOA01_002501"
     CDS             696..1130
                     /locus_tag="NOA01_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633651.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            1747..>4073
                     /gene="esp"
                     /locus_tag="NOA01_002502"
     CDS             1747..>4073
                     /gene="esp"
                     /locus_tag="NOA01_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HCI0633652.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMEDDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
BASE COUNT         1492 a          565 c          777 g         1239 t
ORIGIN      
        1 agaaatatat ctgtttcttt atgaaaataa aatactggaa tatgttaaaa atggagatat
       61 tcgaaattta gaaaacatgg tttttaatct gagtaatgga ataattccaa gtgttagtgg
      121 agatacgatt cggtctgaaa aaaattattc tatcattgtt tttgaaaagt tagcacaaac
      181 gtctataaca ttaggaatgg atataataga agcgtatcaa agtagagatg ctttgataca
      241 agaaaacgaa ttagctgtat cgttacctga ggtcttaaaa gtgagggatt ctgggattgt
      301 ttattatact aaggaaatcg gaaaaactaa gatagagcat ctttctccct taatttcttc
      361 tgtcgttcaa ttcatcggct taaatatcta taaaagaatt actgtaaagg aaatagctaa
      421 ttatttttct gtaagtgaaa ctaaattaag agaaagcttt aagaatgaaa tgcatattac
      481 gatttataat tatatctcta agcgaaaaat ttctacggct aaaataatgt tgaaatcaaa
      541 ccatactatc agtgaagttt ctttaggttt aggattttca gattcttcac acttttcgag
      601 agtattcaaa aagtacgcag gtgtatctcc taagcaatat caattaggtt tagtggacaa
      661 tttcaacaac attagctgag aggttttatt ttattatgag actatatcat ttacgtagtt
      721 tggaggagtt gaggaaattg gattttaaca aaaaattttt ttgggaaatt tctgatttga
      781 gttgtttcgt agaatctgag cgtctaagta atggtaataa tagtgtgtta aatgaatatg
      841 acaaagtagt aattaataaa gtaaaaagtt tatgtgaaga agctagagaa tctatcagtc
      901 atattaatgg tagcaatttt aaacaaacat tatttaatct gattaagcta gatgctaaaa
      961 tatcaagtta tttattcttt ttgatacatg atgaatttat ggattatcaa aaattggatc
     1021 agttaattga aagagaaagc tgggaagatt attacgtggg tctaactttt tcagaaaagt
     1081 taaacaacta caagttaata gattataagt atttgagtga aagcaattaa ttattcatgt
     1141 agagtactag acactttaaa tatggacaat tcaattataa agaaaaagaa tcaataattt
     1201 aatgaggctg tgacaaatgt cattttttgt ttcgctaaaa acgagtgaaa aaatgttgat
     1261 aactccaatc attcgtttaa gattctagga atcattcaga aatgctgtga aaccgatact
     1321 ttataattat aaagaaggtg atttcaaatt ccgaagaagg aatttgaagt caccttttta
     1381 cttatgtcac agtctcattc agtaatgcgg gataaggctc cttattccgt tatttgaata
     1441 ctctgaagtt taatctaaga aagggtaata ttttgaaaac atgcttcatc gcatagaaat
     1501 gaacttaatt tcagaaagaa tacatacata tctttttata tgttttcagc tgctgaatgg
     1561 ttaaaaagca caaatatccg aaaaaaacat gaagaaatgt actttttttg ttgtaatgtt
     1621 catgaaattc tctaagtaaa ttagttaaca taaggttagt aagattccta ttctgtgaat
     1681 aatacgtatc agatttatct tttattctta ctaggaaagt aattattaag ggagttgatt
     1741 tgaataatgg ttagcaagaa taataagaga gtatttctag aaaaaacaaa gaaacgagta
     1801 ttgaagtaca gtatcaaaaa attaagtgtt ggggttgcat ctgttttgat tggtgttggt
     1861 cttgtcttgg gaacaactga actagttaaa gcacaagatg aaatttctcc aagcagccca
     1921 cttgaaacag cgataagctc cgtacaagta ggtgacaaag tagcttcggg gaatactttt
     1981 caggagaatc ctggttatac gaaaaattat aacttttcgg atttacaatt tagtcctcaa
     2041 gaactaactg gagacacact aaaaggcaat acaattggat ttgaagttta cggaaaacat
     2101 aatattgcag catcaactaa aaactgggaa atccgtcttc aattagatga acgattagct
     2161 aaatatgtag aaaaaattca ggtcgatcca aagaaaggaa ttggatcttc tagacggacc
     2221 tttgtaagaa ttaatgattc gctgggtaga cctacaaata tttggaaggt caattatatt
     2281 cgtgcaagtg atggattatt tgctggggca gaaacaaccg atacgcaaac cgctcctaat
     2341 ggggtaatta cgtttgaaaa gagcctagat gaaattttta aagagatagg catagacaat
     2401 ctcaagaccg atcgtttaat gtatcgaatc tacttggtaa gtcatcaaga cgatgataaa
     2461 attgtaccgg gtattgatag tacgggctac tttttaacag attcagatga tttctataat
     2521 agcttagatg tatctgaaaa taatcctgac cagtttaaac atggatcagt aagtgcaaaa
     2581 tatgagaaac ctaatactca aacaaaagat ggttcaggat ccacaggtgc taatggagct
     2641 ataatcttag atcataagtt aacgaaaaat tataatttct catattcatc ttccgcaaag
     2701 ggcacgcctt ggtatgctaa ttacaaaatt gatgaacgat tagttccata tgtagcgggt
     2761 atacagatgc acatggttca ggctgacaaa gtaacatatg atgtttcgtt tgaatccgga
     2821 acaaaagtag ctgatttagc aatcgagcga cgggaaggtc atgaaaatta tggcataggt
     2881 tcaataacta ataatgattt aactaaactt atcgattttg ctaatgcaag tccacgtcca
     2941 gtcgttattc gatatgtttt acaattgact aagccattag atgaaatctt agaagatatg
     3001 aaagcaacag cacaagttga agaaaataaa ccatttggtg aagatttcat ctttgattct
     3061 tggttgtcgg atacgaataa aaaattaatc cagaacactt atggaacagg ttattattat
     3121 ttgcaagata ttgatggtga tggaaaccct gacgataaag aagagagcgg agacacgaat
     3181 ccatatatcg ggaaacctga attagaagaa gtatatgatg ttgacacaac agttaagggg
     3241 aaagtattca tccacgagtt agcgggaaca ggtcacaaag cccaacttgt tgataaagaa
     3301 ggtactgtat tagcagaaaa aactatcgct ccaaatgaaa aagatggggc tccaatttca
     3361 gatactgtag aatttgaatt tacgggtgta gattcaagta aattaatcgc gaaagatgaa
     3421 ttaaaaatcc aaatcgtttc tccaggtttt gataaaccag aagaaggttc aaccgttatt
     3481 aaggaatcac caaaagcggt tgataaacaa accgtggtag ttggatttaa accagatgct
     3541 aaagaatcaa ttcggaataa taaaaactta cctgaagatg cagagtattc atggaaaaca
     3601 gagcctgata cttctaacgt tactgatagt acgaaaggta ttgtaactgt taagatcgga
     3661 aatcgaactt tcgacgtgga tgtagagttt gctgtaaaag cttctcaagc tatggaagat
     3721 gatgcaacat acgtacctat aacaacaact ccagaaacga cagttcaaag tggaaaacct
     3781 acatttgata aaccagatgt tcctttagct aaagatgcct tttcaatttc agatgtctat
     3841 aataaggact tcggtaatgc aagtgttgat gcaaacactg gtattgttac attcactcca
     3901 gctaaaggtg taggagaatc ggagccgatt actggaacaa ttcctattaa aattgtttac
     3961 caagatggtt ctgtaggcac gaccgattta gcagtaactg taagtaaaga tatttatgaa
     4021 aatccaggag aaaacattcc tgcaggctac cacaaagtaa ccttcaccgc agg
//
LOCUS       DAIGOK010000168         4039 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.171, whole genome shotgun sequence.
ACCESSION   DAIGOK010000168 DAIGOK010000000
VERSION     DAIGOK010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.171"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            179..775
                     /locus_tag="NOA01_002503"
     CDS             179..775
                     /locus_tag="NOA01_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCI0633653.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
     gene            899..2698
                     /locus_tag="NOA01_002504"
     CDS             899..2698
                     /locus_tag="NOA01_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCI0633654.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESMKQTDEEITYSDKLLNFVLGVG"
     gene            complement(2976..3188)
                     /locus_tag="NOA01_002505"
     CDS             complement(2976..3188)
                     /locus_tag="NOA01_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633655.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            3650..3799
                     /locus_tag="NOA01_002506"
     CDS             3650..3799
                     /locus_tag="NOA01_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633656.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
BASE COUNT         1188 a          689 c          711 g         1451 t
ORIGIN      
        1 tttaaaaaaa acaagttatt aaataagtct ttagttggat gtttttttga gatcaatctt
       61 ttatacttat gatgaactta caaaattgtt tgttttctgt catatcaaaa aatggcgcag
      121 ttagttgctt ttctagtaaa gtatagttaa caaagaacct ctaaggagtt gatgaataat
      181 gaatattgct gcctctcgaa aactgataaa tttcatatcc attatcggat taggcctatc
      241 aatcgcgctg acgatttatt ttattaattt aggtgttttc aaagatttga atgcgttaag
      301 aggattagta ggtgattcca tcattttagg accgattatt tttgtcttca tccaaatcct
      361 gcaagtagtt attccaatca ttcctggagg gatcagtact gcagctggtg tcctgatatt
      421 tggcccttat gctggattta tctataacta tattggaatc tgcattggtt cgattatcat
      481 ttttctgcta gggcgtcgtt atggaaagcc attcatactt tccatgatta gcgataagac
      541 atataacaaa tatattggct ggctggataa tcaaaaccgt tttgagaagc tgtttgctct
      601 agctattttt cttccagttg ctcctgacga tgcgttatgt ttgatggctg gattgacaaa
      661 catgtcagtg aaacgatata cactaatcat tttgattgct aaaccactgt cgattttctt
      721 atatagtatg gccttgattt atggaggtca atatctaagt ggtcttcttg gataatattt
      781 attttgtaaa attagtcatt gattgacaat gttaaatctg ggaaagggtc tgcaaaacac
      841 actgcaccct ttcctttttc attagatttg cgctacaatg tgaagagcag gtgataggat
      901 gaattatttg aaacgtagct taggtaatgc ttgggacttt ttaaaaggaa cacaagctta
      961 tttccgtgat gtcttgctga tgcatggctt tattttattt atttgtttac ccttattgag
     1021 cagtacgacc cgttttattt tacaccgagg ggcaattgac tatttgtctt atgacaatat
     1081 tccttcaatt ttttctcaac atcctggggt tctactctcg ttaatcgtag ttttgatgac
     1141 gattttactg ttagtttatt tcgaatttac ttttcttttg atgagtgttt tttttattaa
     1201 aaagaaagaa ccaatctctt tgaagcaact cttacacttg acgatacttc agctgaagaa
     1261 agtgagaccc attacttttt tgtttttcct tgcttatttt ttattgattc taccgatcag
     1321 cggattaagt tttaattctg atctactgtc aaaaataaaa atccccgctt tcattatgga
     1381 ttttattttt gctaatcgtt ggatcatcgt cagttccttt ttactcgttt atattttcct
     1441 tggctatatt gggattcgac tgatttttgc tttaccggaa atgattctac gggatcgtcc
     1501 ttttcgagca gccattcggg aaagctggtc attgaccaaa tcgcgtcttt tggccatcac
     1561 gggccaattt attgtgattg gcggtacgat tttactcctt tcttctttag gatatatcgt
     1621 tgttattttg gcacaaagta tggttgaaca atttttccca gactatgcat tgatcagcgc
     1681 tgtgtttgct atgacgcttt tacaagggat tttattattc aatatcgtga tgtctaccgt
     1741 agggattttt tatatcattg ttgatttcat ggatgacgaa ggcttcttgc ctgagatccc
     1801 taagtggttt atcccacaag cccccaactt gcgtttctct gcgctgaaaa acactggttt
     1861 gactcttttt gcagtttttt tcggtattgg cgtctgtctt tacaatatgg attatctgac
     1921 ttcagctgtt cagacaaaac cagtgactgt ctctcacaga ggtgtgagta cccaaaatgg
     1981 ggcgcaaaat actttagaag cacttgaaaa aacaagcaga gactatcatc ctgattatgt
     2041 agagatggat gttcaggaaa caaaagatgg tcatttcgta gtgatgcatg atgcaaatct
     2101 tcgtcactta acaggggtaa atggaacacc acaggatctt acattgaaag aattgacaaa
     2161 tcttacagtt actgaaaacg gagtgcaagc ccctgtttgt tcttttgatt cttatttgga
     2221 aaaagcacat aagctaaacc aaaaattact tattgagatc aagacctcta aaaaagattc
     2281 ggatgacctt gtcgaacgat ttgtccataa ataccgcaaa gttatcttag agaaccatca
     2341 tattttgcaa agtttgacgt accaaaccgt ttcagattta aaagaagcag aaccaaactt
     2401 ttatgtgggc tacatcttac ctttcaatat tgttgggccc cctgtgacgc ctgctgattt
     2461 tctgacaatg gaatatagta cgatcaaccg taacttcatt aattctgccc accaagacgg
     2521 aaaagaagta tatgtatgga caatcaacga ttcagatgct atgagtcgca tgatgtttta
     2581 tggtgtggat ggtatcataa ccgatgagat gggactacta aatgaaagta tgaaacaaac
     2641 agatgaagaa atcacttatt cagataaact gctaaatttt gttttgggag tcggctaatc
     2701 aaaaatcgtt aatgaaaaaa gtctttatac aacaaaaaac gcataacctg ccaccgcagg
     2761 tatatgcgtt ttttgtttga cgctcataat gtgtgcttaa ctttctcttg attccttcat
     2821 cgcacgaaca atagctaaaa tagcaggaat tgcagagatc actgcaagaa taattcccat
     2881 tttccctgaa ctttttcttt ttggcatatc ttctcgctcc cttctgtctt tatttatatt
     2941 ataagggagt tcttgcgaat tctcaaaaat actgtttaag aaaccaattc agcaggtgcg
     3001 ctaatatcac agaattttac gagcatgcga tacttgcttt cattgataag ttgatagtct
     3061 cgttggtcat aactttcaac atacacgaca gccgtgttga tatatgtacg ttcaatacag
     3121 ccgattagtt catgagatac tccgatcggt ttgcatatat aagatcttcc tacttctgct
     3181 tttaccattt cattccctct tttctttatt tttattttgt tttttcttta ttttataata
     3241 acaaattacc ttgtatttga aaacgttttt atccattttt ttatttttta ttttgagtat
     3301 ctttctgctt tttagataac tattttttaa cacgatctta gacgttgtga caaaaacgtt
     3361 tgtttatatt cctatgttgt tcgtgcgttt ttttctttat attgctaagt tatttataaa
     3421 gaggtgagca tatgatttat attcaagcaa tagtatttgt aaccttgatg attgggctac
     3481 ttatcggatt aaaagaaaat agctaaacca tttaagaaaa aataaataag gaaaatgata
     3541 aatgcttttt aattgcatca aaaaacgtac gatgctaaac ttggtataag ttcgaaagtt
     3601 taaaggaaac tttcaagaac taaattacat tacaaaggag atgaatttta tgacgtttac
     3661 aaaaacaaat atgcaatacc gcgtagcttt tgatacaact tctaaccgtt ttatggcgat
     3721 tgatgcacaa aacgaaaata acgttgcgtt tggtattaca atcgaacaag cagtccgagc
     3781 attaaacgaa tctagataag gagtgaacca ttccaaatgg cgactgatga gatctcggaa
     3841 aatcaataaa agacgtttgt aacggtaatc cgcgacaaac gtctttttgt attcattaca
     3901 tatatttgga aattgtctct ctttatctct agacctaaac aaaaacacaa gttctaatat
     3961 caaccatctc gttttttatg acaattttgt tagctgtttc gatgataata ttccgttatt
     4021 atctgtataa tatttaata
//
LOCUS       DAIGOK010000169         4034 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.172, whole genome shotgun sequence.
ACCESSION   DAIGOK010000169 DAIGOK010000000
VERSION     DAIGOK010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.172"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            73..234
                     /locus_tag="NOA01_002507"
     CDS             73..234
                     /locus_tag="NOA01_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCI0633657.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            253..1530
                     /locus_tag="NOA01_002508"
     CDS             253..1530
                     /locus_tag="NOA01_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI0633658.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1540..2196
                     /locus_tag="NOA01_002509"
     CDS             1540..2196
                     /locus_tag="NOA01_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0633659.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2196..3572
                     /locus_tag="NOA01_002510"
     CDS             2196..3572
                     /locus_tag="NOA01_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCI0633660.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3702..>4034
                     /locus_tag="NOA01_002511"
     CDS             3702..>4034
                     /locus_tag="NOA01_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCI0633661.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1612 a          440 c          704 g         1278 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
     1261 gagttaatat tcatatcgat accaagtata atatcttcct tatttttagg aaatgtatta
     1321 ctaaaagtaa ttgtagatgg attcattaac tcagaggatt caatgatttc cggtggaagt
     1381 ttaataaata atagtagttt tatgtcaaat ataacaacac ttggacaaag ttatttaata
     1441 ttaataagta ttattgtttt atcagttgta tttgcttctt cattaatgtt aatcaagaag
     1501 cctaaagaaa tcttatcaaa aataagttag gagaaaataa tggatatatt agaaataaag
     1561 aatgtagcat atagttatgc aaattctaaa gaaaaagttt tgtcaggagt aaatcaaaaa
     1621 tttgaacttg ggaagtttta tgcgatagta ggaaagtcag gaacaggaaa atctacactt
     1681 ctttccttac ttgctggact tgataaacct caaacaggaa aaatattgtt taagaatgaa
     1741 gatatacaaa ataaaggata tagtaatcat agaaaaaata atatatcttt agtatttcaa
     1801 aactataatt taatagatta tttatcacca attgaaaata ttagattagt aaataaatca
     1861 gcagatgaaa gtatcttgtt tgaactaggt ttagataaaa aacaaataaa aagaaatgtt
     1921 atgaaattat ctggtggaca gcaacaaaga gtagctattg ctagagcatt ggtatcagat
     1981 gctccaataa tactagctga tgagcctact ggtaatctag acagtgttac tgctggagaa
     2041 ataattaata tattaaagac attagctaaa gatagaaata aatgtgtaat agttgtaaca
     2101 catagtaagg aagtagcaga ttctgcggat atcattttag aactaagtgg taaaaagttg
     2161 aaaaaagtaa ataaaatgaa tttggaggtt gaataatgat aaaaaatgca tttgcttatg
     2221 taactagaaa aagtttaaaa tcattaatta tcatattagt tattttatct atgtcaactt
     2281 taagtctcat tagtttatct attaaagatg ctacggacag agcttcaaaa gaaacatttg
     2341 ctaatataac aaatagtttt tctatggaga taaatagaca agtaaatcca ggaacaccta
     2401 gaggtggggg aaatgtaaaa ggtgaagata ttaaaaagat atctcaaaca gatagtatag
     2461 actcctatgt aaaaagaata aacagtgttg cagatttagt tgattatgat attattgaaa
     2521 ctcaagagac tcttgcgaat caatcacctg aaagagcaaa gaattttaaa agaacagtta
     2581 tgttaactgg ggttaacgac tcaacaaaag aaacaaaatt tgtatcagaa gcatataaat
     2641 tagtagaagg aaagcattta gaaaatgaag ataaaaataa aatcttaatg cataaagatt
     2701 tagctgaaaa aaataacctt aaagttggag ataagataaa aataaaatct aatttatttg
     2761 atgctgataa tgagaaaggt gcagatgaaa cagtagaagt agaaattaaa ggtctattcg
     2821 atgggcataa tagcggtgta gtaagtgcag cacaagaact atacgaaaat acattaatca
     2881 ctgatattca tacagctgct aaagtttatg gtaataccga agatacagct gtataccaag
     2941 atgcaacatt ctttgtaaaa ggtgataaga atcttgatag tgtaataaaa gatcttggga
     3001 aattagatat aaattggaga gaatataatt tgattaaaag ttcatcaaat taccctgcat
     3061 tacaacaatc tatatcaggt atttactcaa tttcaaataa attatttttt ggctcattaa
     3121 tatttgcagg agttgtagtt tcattattat tattcttatg gatgaatgct agaaagaaag
     3181 aaatagcagt attactatca ttaggtatat caaaattaga aatttttggt caattcctaa
     3241 ttgagatggt atttatatca atccccgcat tcgttggttc ctatttctta gctcaatata
     3301 cagctgacaa gcttgggaat aatatattga ataaggttac tggtgatata gctaaacaaa
     3361 tagctaaaca atctgcatct agccaattag gtggtggggc agaagctgaa ggatttaaca
     3421 agacattatc aagtttagat atcaatgtat tacctaaatt catgatttat gtagttatat
     3481 ttatgagttt agtacttctt gtatcattga ttatttcttc attcaatata ttaagaagga
     3541 atccaaaaga attattaatt gacaataatt aaaattttcg gtgcttttta gcaccaaaaa
     3601 tgagagtgta atataaaccg tgtaagtaac catcaatcct aggcgggtgg ttgcttcacg
     3661 gtttttattt ttctaaaata agatcaagag gtgattacac gatggctaga aagaaaagaa
     3721 atcctgatgc tgagaagtta gctgaatcca ttttgaatac ttatcaaccc gaatctgtag
     3781 aggatatgca agatgtttta aaagatgtgt ttggtccctt atttgaaaaa atgcttcaag
     3841 gagaattgaa taaccattta gggtatgacg cccattctaa agagccaaag aaacatgaca
     3901 atcgacgaaa cggttatggc aataagacgc ttaaaacgag ctttggtaaa gtaaacattg
     3961 atgttcccag agaccgggag gcttcttttg aacctgagtt gattcctaat agaaaacgag
     4021 atgtctccga catc
//
LOCUS       DAIGOK010000170         4022 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.173, whole genome shotgun sequence.
ACCESSION   DAIGOK010000170 DAIGOK010000000
VERSION     DAIGOK010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.173"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            18..515
                     /locus_tag="NOA01_002512"
     CDS             18..515
                     /locus_tag="NOA01_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633662.1"
                     /translation="MSYLIVCLIIIICLHHLFLSKTIPRYSKNKKAEKFCLLLNKFTL
                     IAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFLMSLKPPKSISLC
                     LVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYIGYFPNKNNNFYF
                     YRNKL"
     gene            complement(641..1318)
                     /gene="nth"
                     /locus_tag="NOA01_002513"
     CDS             complement(641..1318)
                     /gene="nth"
                     /locus_tag="NOA01_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCI0633663.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1325..2023)
                     /locus_tag="NOA01_002514"
     CDS             complement(1325..2023)
                     /locus_tag="NOA01_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCI0633664.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2291..3475
                     /locus_tag="NOA01_002515"
     CDS             2291..3475
                     /locus_tag="NOA01_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HCI0633665.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3481..3924)
                     /locus_tag="NOA01_002516"
     CDS             complement(3481..3924)
                     /locus_tag="NOA01_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCI0633666.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT         1057 a          785 c          655 g         1525 t
ORIGIN      
        1 tgaaagaagg cttcattttg agttatctga tagtatgctt aattatcata atttgtctgc
       61 accatctttt tttatcgaaa acaatacctc gttattccaa gaataaaaaa gctgaaaaat
      121 tttgtttatt attaaataaa tttactttaa ttgctcctat ccttgcattt attatttttt
      181 ctgtactttt aagcactact cttaaaggaa aatttatgga aagaagctct catgcaatga
      241 ttctcacttt tttatggttg ttgttcacca gaatctatat ttttctgatg agcctgaaac
      301 cgcccaaatc cattagttta tgtttagtta taaatggtat ttttttatta tcacttatca
      361 tttttattac acctttagat agatacgtca cttatttata taatcctttg gaatactgga
      421 cttattttat aggtatattg gaaggcatta ttttctacat cgggtatttt cccaataaaa
      481 ataacaactt ttatttttat cgcaataaat tgtaataagt taccatccta gctactgagt
      541 atgtacattt actttttcat gcaaaaaaga taaacgaaac agcttttctc cataataaca
      601 agataattca tacatttatt tgcaattaaa gaaaccacgt ctacaggcgt ggtttctttt
      661 ttagcgcctt ttcttttaga cgcatccgat ttttcccatc ttgacatatt gatagcaaag
      721 gacatacctc gcacttggga tttcttgccg tgcaatgata tctaccaaaa aaaatcagtg
      781 tatgatgtgt ctttacccat aattcttgtg gtactttccg catcagtgtt tcttctactt
      841 ccatgaccgt agcatctagt ttgcagatcc tcaggcgttt tgagacacgt tccacgtggg
      901 tatctactgc gattgctggt atcccgaatg catcccccaa tacgacattt gctgtctttc
      961 tgcctacacc aggaagagac atcaattctt ctcttgacgt tggcacttgc ccatcgaaac
     1021 gttcaatcag ctgctgtgca cacgctttga tgttttttgc tttatttcga tacaaaccaa
     1081 tcgtttttat tttaagaatg atttcatcga ttgaagcttc tgctaacgca tcgggtgtcg
     1141 gaaaagaggc aaacaaatct ggcgttgctt tattcactga tacatctgtc gcctgggcac
     1201 ttaagatcac tgcaatcaat aactcaaatg gattattgtg cttgagttct ccatgtgctt
     1261 cgggaaacat tccatacatc gtttctagag cttccattgt tttttgtttg cttaacatat
     1321 tccgctactc ctccaaccaa tttttcagtg agactttcgg caattcttct tgttgagctg
     1381 actgttctac ttctctttga agaagctgct gttttcttcg tttttggtct tcttccactt
     1441 gttgttttgt acgtagattt tttctttccc acgatagcaa aatgcggtca acgtaattaa
     1501 aactataagc ctgatttaat acggcttccc gcagtgctag cttcaaaatt tccggctgat
     1561 aatggtcttc ttctagccat tgtccgatcc gctggaactc gatcgaagaa agcgggcgtc
     1621 caaattcttg ttcaaataat tgatacactg actggatttc tttttcttgt gttttttctt
     1681 cgtatttttt ttcttgtgtt tttaaatact cacctaatag atcatagatg gggtaaagat
     1741 tatatcgatc tgctttttta ccgttgacaa tcgtcgtttc tatcttgata aatccatttg
     1801 ttacgagacg atccagtatt tgaaaaatct gatcttgttt gattcccata tccatagcaa
     1861 tcatctgcaa atcagggaaa gtatctcctt caagctgtgc catatgcaac tgtagaacaa
     1921 ataaaaattc ttcagattgt agaccgatcc tatgataatt tttaagtaaa agattagata
     1981 tgactgtttg tccagcgctt aagtaatttt ctaaattcaa catgatttct cctccgctca
     2041 atcacagtat acgtaaaaaa aacatgggaa gcaagaacag gtttcttttc tatttacttc
     2101 actgatcggt cagatttaca atgtcgtcta cttgattttt gtttgttcat gtttttgatg
     2161 cgtatcttca cttttcggaa taaataaatt aagtagattt cgctgttcct tagaatgttg
     2221 acaaggaaac aaaattacta tacactattt ttgtttccaa gttaacatta ttgcataaag
     2281 gagtttttac atgtacagtt tcacaaataa acattctaaa gtttttggat acgtctctgt
     2341 cttattcact ggattaggat ttactatcgt cagtcctgta cttccgtttt taacgctgcc
     2401 ttattctcac tccatacatc agcaagcttt ctatatcact ttgctcatgt ctgtttatgc
     2461 tctagctgcc ttcttatctg cccccattct cggtagctta agtgatcatt ttgggcgtca
     2521 tccgatcctt attatcagtt tattaggatc aagtatcgga tatttgcttt ttggtttagg
     2581 aaattcgata tggatgttgt tccttggtcg tatcattgaa ggattgacgg caggagaaat
     2641 ctccacctta tatgcttact ttgccgacat cacggaacct aatgaaagaa ccaaagtttt
     2701 tggatggatg ggtgctttag tcggtatagg aacaacgctg ggtcctatta tagggggtct
     2761 attagctgag ttggggaatt ctgttcctat atttattgga gcactcttta ctttcttaaa
     2821 tgccgtttat ggctacacat ttatgctaga aagccttcca ataaaaaaac gttcagtaga
     2881 tttaagtttc tcccatgtaa gaccttttca ccaattacaa caattattca agatttcatc
     2941 cgttattcct ttattgacag caggttttgc tgtttggttg gcagctggct ctcttcaatc
     3001 tatcttttct caattttcga ttgacgcctt tcagtggaag gctgggttgg ttggtttaag
     3061 cttctctttg attggaatct tggatactgt ctcgcaattg ttcattatgc ctcgattgct
     3121 caaaaaattt tcagaacagc agatcacaag aatagggatg ttcagtgaaa tcctcgccta
     3181 tctattcatc acattatctg gaattcttct actgccgatt ttgtttttat ttgggattat
     3241 ctgttatggt ttcggtgatt ctatttttac tcctgtattc aatggccaac tatccaattc
     3301 tgtctcagat aaccagcaag gtcttgtaat gggcggtacc caaagtatac aatctctatc
     3361 gcgcgtcatc ggcccgctaa tcgcaggaca gctttatgct gcagcaccat ttcttccgac
     3421 ggtattcgga tttttgctta tgatttttgc ctattatcac tacaataaaa aatagtagct
     3481 tcatttgcta cttcgtataa attctctgta tcgaccgata aaacgaaata ctgtgtcaaa
     3541 ttcttctttt gacatatttt caaatacagt agcatctcgc tgtacccatt tttgatggag
     3601 ttgctcgtgt ctattcctta ttttttgtcc gattggcgtc aattgaaaat agatttcttt
     3661 tttattctct tcgctttgat aagtgtttat gatccctcta gctatcagtt tttttgttag
     3721 cttactgatg gctcctctag tcatgtaact agcattcgct aattttgtca cgttaggatc
     3781 agggattttc ccgatatgtt caatcacttc tagttcattt agagtaaatc ctgcaaattc
     3841 tttttccatt tctggtttat tacgatgaat aatctcatta gctaaatcca gatattcttc
     3901 tattagcagt tgtcttttat ccatttctac accttctttt tctttcatta tattgttttt
     3961 ttgtttccta ttcaacaaag ataaaaatat tttgtaggaa aacccaatct gaaaccaaga
     4021 aa
//
LOCUS       DAIGOK010000171         4013 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.174, whole genome shotgun sequence.
ACCESSION   DAIGOK010000171 DAIGOK010000000
VERSION     DAIGOK010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.174"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            225..668
                     /gene="rplM"
                     /locus_tag="NOA01_002517"
     CDS             225..668
                     /gene="rplM"
                     /locus_tag="NOA01_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCI0633667.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            684..1076
                     /gene="rpsI"
                     /locus_tag="NOA01_002518"
     CDS             684..1076
                     /gene="rpsI"
                     /locus_tag="NOA01_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCI0633668.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(1165..2391)
                     /locus_tag="NOA01_002519"
     CDS             complement(1165..2391)
                     /locus_tag="NOA01_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCI0633669.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGITSEKYQKVFPTRQLAIKA
                     ENAMKRKIETVLREENANSLELKGKIKFKEFYESKWLPRYELGQTTRSKRAPSYVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQCITAPKKIALEERRIREGNQALSSEELIAWIEAVNTDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFEKQTVRLKHALDKYQKKKFTKGRKDTV
                     IQVPEIVMALLSKWKSVQAEQLLRLKINQTPDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPELAHLSPHKLRHTYATIARQGGANMNQISSALTHSDISTTRIYVNTPDVV
                     DKTVFEAFQRGLNKCD"
     gene            complement(2463..2621)
                     /locus_tag="NOA01_002520"
     CDS             complement(2463..2621)
                     /locus_tag="NOA01_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCI0633670.1"
                     /translation="MNKKQTCEFLGIDNNTLDKWIRIYNFPVIRIDGIVRFDIEDIKD
                     FMNTFKTI"
     gene            3309..>4013
                     /locus_tag="NOA01_002521"
     CDS             3309..>4013
                     /locus_tag="NOA01_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCI0633671.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFI"
BASE COUNT         1307 a          645 c          690 g         1371 t
ORIGIN      
        1 ttgtcacagc ctctttttgt ttttcatttt gacaggaaac gaccctttta tataaaagaa
       61 agaaaagaag taaagtttca tataaatttt tcaatcaagc agaaaaacgt tgacattgac
      121 tagattagcg ggtaatatgt taaatggtat tgtttgcccc acaaagagcc ccggaaactc
      181 gaagtgtatg tcaaacatca gaacttaaat tggaggaaaa taacgtgcgt acaacatata
      241 tggccaaacc aggcgaagta gaacgtaaat ggtatgtagt agacgcaact gatgttccat
      301 tgggacgtct ttcaacagtt gttgcatctg tactacgtgg taaaaataaa ccaactttca
      361 cacctcattt ggatactggc gatttcgtca tcgtcatcaa tgcagacaaa gtgaaattaa
      421 caggtaaaaa agcgactgac aaaatttatt accgtcacag caactaccca ggaggattga
      481 aatcaatcac tgctggcgaa ttgcgtgcta aaaattctcg tcgtttgatc gaaacttctg
      541 taaaaggtat gcttcctaaa aacactttag gccgcaaaca atttacaaaa ttgaacgtat
      601 acggtggagc agaacatcca catgcagcac aacaaccaga agttttagac atcacaaact
      661 taatctaata ggagggaatt tcattggcac aagttcaata tatcggcaca ggccgtcgta
      721 aaaacgcagt tgctcgcgta cgtctagtac caggaactgg caaaattact gtaaataaaa
      781 aagatgttga agaatacatt ccacatgctg acttgcgtga agtaatcaat caacctttcg
      841 ctgttactga aacaaaaggc gcatatgacg tatttgttaa cgtaaacggc ggaggatacg
      901 ctggacaatc aggagctatc cgtcatggta tcgctcgtgc attgctagaa gttgatcctg
      961 acttccgttt agctcttaaa cgtgctggct tacttactcg tgacgcacgt atggtcgaac
     1021 gtaaaaaacc aggtctcaaa aaggctcgta aagcatctca attctctaaa cgttaatatt
     1081 atttatatat caatgtttca aggcactttt caattgtttg aaaagtgtct ttttcttgtt
     1141 taagaacaca tttaagaaca catactaatc acatttattc aatcctctct gaaaggcttc
     1201 aaaaacagtt ttatctacta catctggtgt atttacgtat attctcgttg tagaaatatc
     1261 tgagtgtgtt aaagcactag atatttgatt catatttgct ccgccttgtc tagctatagt
     1321 tgcataggta tgacgaagct tatgtggaga taaatgagcc aattcaggat gtcgtctttt
     1381 aattgaattg attcgatagt ttaggtaatc agcgtgaaca gggcaattta cttctccgga
     1441 tggtttagta taggtaaaga gatactgatc tggtgtttga ttaattttta atctcaacaa
     1501 ttgctcagct tgcactgatt tccatttgct tagtaatgcc attacaattt cgggtacttg
     1561 aattacggta tcctttcgac cttttgtaaa tttctttttt tgatatttat ccaaagcatg
     1621 tttaaggcga accgtttgtt tctcaaagtc gatatatttc cattgcaaag catacgtctc
     1681 actttttcta tcaccaagat atagggtaag catgaaaaga gtatagtcat gaagagtaag
     1741 caagtgatta ttcaagtcag tgtttactgc ttctatccaa gcaataagct cttctgacga
     1801 taatgcttga ttaccttccc gtatccttct ttcttctaaa gcaatttttt taggtgcagt
     1861 gatacattga ataattttgg tagttcggtt aaattcaata taatttagta tttctgcaat
     1921 atcaaacata gaacggacat atcctttgat aatttttata tttgcgtatt ctttcgattt
     1981 ttttgttaat tcatctgata ttatctcagt atttgaattt agatagttca ttgcatactc
     2041 accaaacatt ggaaggatgt gcaatcggaa tatatcttta gtattactaa ttgttacgta
     2101 acttggagct ctttttgatc tagtagtctg acctagttca tatctaggaa gccattttga
     2161 ttcatagaat tccttaaatt ttatttttcc ttttaattct agtgaatttg cattctcctc
     2221 tcttaaaact gtttcaattt tccttttcat tgcattttcg gctttaatag ccaattgcct
     2281 agttggaaat actttttgat acttttctga tgtaattcca gtgatttctc tgaatttttt
     2341 tggatacctc agattagttt tatatttccc atttgaaagt tttgtaggca aaattcctca
     2401 ctcccttaaa aagtaaggca gtatatttcg ctattcttag tatatgcggc gagaagaaat
     2461 tttcaaattg ttttaaatgt attcataaaa tctttgatat cctctatgtc aaagcgaact
     2521 ataccgtcaa ttctgattac tggaaaatta tatatacgaa tccacttatc taaagtatta
     2581 ttatcaattc ctagaaactc acaggtttgt tttttattca agtagactct caattttata
     2641 aaatcatctc ctaaaaaaga ttagaatttt tgaattgctt tgggtaagca cataggaatt
     2701 tcacctctcc ccagtttacg gcgagcctta cggaagtatc attatttgct gctattgtca
     2761 tggcattttg ttttcccaga acaaaaagaa atctctaagt atttgtcgct aatcaggtca
     2821 tggcgtactt gagtttctag ctcgaatagc agggatcgtt attcaaaaat tgtatttaat
     2881 tgtcaatgat caaaaatagg ttttttccta ttcactatta ataacgataa acatgtataa
     2941 aaagacacac tacgaaaaaa gttaaatgga gtttactgat tttcatattt gtatatggaa
     3001 cagacatagt cttgaataat ttttataaat gttttgatgc ttatcaactt attcaacccg
     3061 ctatccactt taagtataat ttttccatac gccaataaag tagttttagg agcatgttct
     3121 catttgagaa cgctcctttt ttatatattt tgaaagctac ttactatacg cgtagtttca
     3181 aatagttttg tcattctaat cttcagcaac agaaaaaaat ctaaattata aaatttaggg
     3241 ttgactaaaa tataattttt gtataccata tgatcgtgat aatcattatc atttagaagg
     3301 agaaagttat gttagaagtg aaaaagctta atgtttgtta taacgatttt ttagcattga
     3361 acgagatttc tttaaaaata caagaaggtg ctttaacagg tattattggt ccaaatggtg
     3421 ctggaaaatc tacattgctt aaagcaatgt taaatattat ccctcatcag ggagaggtct
     3481 ccattaataa tgaagatatc aataaaaaac tggtgaagat tgcatatgta gagcaaaaag
     3541 ctgatattga ttttactttt ccaataaaag taaaagaatg tgtatctatg ggaacatatg
     3601 ccggaatgaa agtttttcaa agaattaaaa atgcagaatg gcaaagagtt tcaaaggctt
     3661 tggaaaaggt cgatatggga aaatattcta atcatcaaat aggagaatta tctggaggac
     3721 aatttcaacg tgtactttta gcaagatgtc ttgctcaaaa tgcggatttc atatttttag
     3781 atgaaccatt tgtagggata gacttagtta gtgaacgaat aattatggat acgttaaaag
     3841 aattgaaaca tcagggaaaa acagtgctaa ttgtgcatca tgatttaagc aaggtaaaga
     3901 agtattttga taatattatt cttttgaatc gaaatctaat tgctcacgga agtgtagaat
     3961 ctgtatttaa tgaagagaat ttaaaaaaag cctatggaga tactatattt att
//
LOCUS       DAIGOK010000172         3894 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.175, whole genome shotgun sequence.
ACCESSION   DAIGOK010000172 DAIGOK010000000
VERSION     DAIGOK010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.175"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(<1..353)
                     /locus_tag="NOA01_002522"
     CDS             complement(<1..353)
                     /locus_tag="NOA01_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633672.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRINESDYNKRDQVLL
                     VKLNEEISVEEAVRT"
     gene            complement(613..1161)
                     /locus_tag="NOA01_002523"
     CDS             complement(613..1161)
                     /locus_tag="NOA01_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633673.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1162..2019)
                     /locus_tag="NOA01_002524"
     CDS             complement(1162..2019)
                     /locus_tag="NOA01_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HCI0633674.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2305..2592)
                     /locus_tag="NOA01_002525"
     CDS             complement(2305..2592)
                     /locus_tag="NOA01_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCI0633675.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            complement(2582..2911)
                     /locus_tag="NOA01_002526"
     CDS             complement(2582..2911)
                     /locus_tag="NOA01_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCI0633676.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            complement(2988..3260)
                     /locus_tag="NOA01_002527"
     CDS             complement(2988..3260)
                     /locus_tag="NOA01_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCI0633677.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            complement(3260..3523)
                     /locus_tag="NOA01_002528"
     CDS             complement(3260..3523)
                     /locus_tag="NOA01_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCI0633678.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            3757..>3894
                     /locus_tag="NOA01_002529"
     CDS             3757..>3894
                     /locus_tag="NOA01_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HCI0633679.1"
                     /translation="MRSSKYTEHYTDTFITFKEIMRTVSNIYHNCVPDKIKNRRNTDQ
                     LK"
BASE COUNT         1165 a          784 c          498 g         1447 t
ORIGIN      
        1 aatgttctta ctgcctcttc cacactaatt tcttcgttca atttcactaa taatacttga
       61 tctcgcttat tataatcgct ttcatttatc ctaagagtca aatctttttc caccataact
      121 tttacaccca ataaattttg cggtttattt ttctctgttg aaattctttg tgtagccttt
      181 aaactgacaa ttagctcatt gggtacatac tctatttgag agtcatctaa ttggctcata
      241 atcctttctt tttggttagt atctgcagtt acttgtttag tatgagccat gaacccaaac
      301 atactcaccc caacaaccaa tcctaaatat attttctttt taaacttttt catctacatc
      361 tcctcctaat agttttattt tagagttagc tctattactt atttactaac tcaacaacag
      421 attatcatac acttgttaat gaaatcacaa tatctcatta aaacacaccc taaaatcata
      481 accgttccta tgctcatttt ttggattttt taatcacgta accagaaaaa taaatcccct
      541 ctatttcacg caaatcaaag aagaactaag taactaccgt ttaaaggtag ttacttagtt
      601 cttctttgat tattatctaa ctttattatt cattccccac cctttgcgca gtgagtcttc
      661 ggtatcttgt tttatctgac tggtagttct tattatttct ccacgatcat ctaattctgg
      721 aacaattgga tatttttctc tgaacaagtc tagttctttc accacgtcat taaacaaatc
      781 ttgcttatct tcttgcgtaa gattctgttt aatatactca tctaaagctt ttctaagcaa
      841 ttcatctctt gtcatattag tctcatactt ttctactaaa actctggaag tcgcatttaa
      901 caaagtcgac agttttaaag gatgcttggt ggaataaacc ttatcaaact ttctgattgg
      961 tgcgccaact ttttttgttc ttcctctact ctgtctttca acattggagg aagtttttat
     1021 ttcttgaact tggtgcactt ctaattgctt attttcagat ggtgtaattg cttgctgctt
     1081 tttctttgct tctgcttctt tttttatttt ggcgagtcct ttgttgttcc caaaaatatc
     1141 aagtcctttg atgtcagcca attactccac ctccgcatac caattctcgt gttcttccaa
     1201 ttctttcaaa atactgataa ataattcgtg gcttttgcga tcccattgtt ttaagagacc
     1261 tttctttgtc ttctccatcg taatacctga tccgtcgtat cgttttaagc gcgcattttt
     1321 gagtacaaca tttttaatca aatgttcccc ataaatctct tgggcttgtt tatacatttc
     1381 ctgatctact gcgtcaccgc tttccaacat gaaaggaata acaccagcaa catataaatt
     1441 tgtatcaaat tctttcacaa aaaagtccat atactcaaga tattgcatcg catttctcat
     1501 cgattttact tgggtctgta agacgacgat tgcccaatcc gcaatgtaca tcgcggattt
     1561 actatataca gaaatagaag gtgggacatc taaatagatg acgtcaaact catcaaataa
     1621 tggttctagc ttttctttca tatatttgaa ttgggataat gaatcattat attggtcttt
     1681 gatccaatca tcatacattt caaaggacat gtctgctgga agtatataga ggttattgat
     1741 tactttatag acagcctctt taggtggaag attgtttatc aacacgtcag tcacagtatt
     1801 ttcgtaaaca atcggttcac cttctccgcc tccttcttcg ttagtgaagt tcatatgcgt
     1861 taagttcatc acatcagaag tatttccttg catatccaag tcaattatca aggttctttt
     1921 tccttcatta actgctgatt caaaccctaa catttgcgcg cacgtacttt tacctacgcc
     1981 acctttaaaa ttaccgatta catatttctt cgctaccacg ttcctacctc ttttctttta
     2041 gcaatattca tttgatacta ttataacata tttattttta gttactacca aaaaaaagat
     2101 atatatgtac taccttataa ttaataagta aaaggtagta cataaataag gaaaagctga
     2161 aatatattga tttatagctt tcctcaaagt aatgacgtta tttaatttat cgagaagcag
     2221 agaatactcg cgactttagt catgtagatg aatcagatct tcgataaaaa agaagccgtc
     2281 cttcttaaga acgacaacaa atcactagat taattttttc aactgttcat aaaagttttc
     2341 atgcgttcca cataacaata ctggaataac cttgttttct ataatctcgt aagctactct
     2401 atatgttgtg cccgcatatt tgaacccact agaccaaata cctgataaat ccccagtttt
     2461 ttgttcaccc ttatatggat caatcgcaat ttcatcataa attaaattca aaaatttttc
     2521 tttcaatggc ttctctttta gcttttttaa atacttaatg acatgttttg aaggaacgat
     2581 ttcataagcc aatttctgtt gccaactcct ctttagtcat tactttagaa ttagtaagtg
     2641 tatcttcagc aatatctcta aaggaagaat gcattttgtt ttttctattc ttaaattcta
     2701 caagaatctc atctttatcg tagccttctg caattatatc agacaaaata tcttcgctaa
     2761 aatcataaaa ttctttttgt ggttcaacaa attcataaag aataccattc tctaccaact
     2821 ttgcttcaac ttcgactcca ttaggaacat taaaatcttt aggtactgtc aacatgattg
     2881 aattgccttg ttttcttgtt tttgtaatca tttaaatccc tcatttctaa cagtatgtaa
     2941 ttacttagta aatacattat accacgagat aatgtattta caagtaatta ctgtgactcg
     3001 taatgcgtcc ttgctgaata aatttcaacc acgtgttcat cttcatctac cttataaaca
     3061 acccggtgtt gtctattcaa tcttctagaa tacctacctt catgtgtagg ttttaacttc
     3121 tcaaatgatt gggtagggag atagggatct tcctttaaag tctgaataac cttctcaaat
     3181 tgaggtttca aattagactg tttgattttc tttaaatcag ctttagccga gtttttaatc
     3241 atcacagaat agttattcat tataagttat cccaatcaat ttcatcaaca ttagtaaatc
     3301 cactatcatc tttttctcgc ttacgaacaa cacccaaagt gcctatttgc tcaagtaata
     3361 gcgtttcttt gattgctttc caatcatcca aaccaataag caccgcatta ttttcactgc
     3421 gctcactaat gatttcaatt tccgtatgat tctcatttac atctttcaac aacttataaa
     3481 aatttttccg tgctgagcta ggatttaaga ctgtttttgc catatctact ccccctctaa
     3541 tttatatctc cattctaacg tacttaatta agtacgtaaa ttcgattgtt ttgtttaaat
     3601 tggagtacca aaacagcatt gtaaagtgtt atccgtttcc tttatacccg tggtgctagt
     3661 taattttttc ctagaggaaa atgtctaaac atgttggtaa atcaacaaaa aaacgcatag
     3721 agtagtctca aaactcccaa gaaatgagga ctacatatgc gttcatcaaa atatactgaa
     3781 cattatacag atacttttat aacattcaag gaaattatga gaactgtttc taacatatac
     3841 cacaattgtg taccagataa gattaaaaac cgaagaaata ctgatcaact gaag
//
LOCUS       DAIGOK010000173         3807 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.176, whole genome shotgun sequence.
ACCESSION   DAIGOK010000173 DAIGOK010000000
VERSION     DAIGOK010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.176"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..859
                     /locus_tag="NOA01_002530"
     CDS             <1..859
                     /locus_tag="NOA01_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCI0633680.1"
                     /translation="DENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVKLLVKRKN
                     GFNKSKMQMFPNFYYLLKPVLCKDDVYKINKITHQENRQKNLIVQNLNYGTNQYSVDN
                     SKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLLESLSTKNNKLD
                     EELIETFIEENLLKQIWGESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAEKEQKIQL
                     KIIYEICNNKYFVDHVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNKALELIEL
                     QISEGIDKQ"
     gene            935..1153
                     /locus_tag="NOA01_002531"
     CDS             935..1153
                     /locus_tag="NOA01_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633681.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            1140..>3807
                     /locus_tag="NOA01_002532"
     CDS             1140..>3807
                     /locus_tag="NOA01_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633682.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGL"
BASE COUNT         1466 a          516 c          739 g         1086 t
ORIGIN      
        1 tgatgagaat caaaaaattt ttttgatatt tgaaatcaaa aatctcgtag aaatttcggg
       61 aatgtcaaaa aatacaatct tatcagcact agatgaactt caaaaagtta aacttcttgt
      121 gaaaagaaaa aatggcttca ataaaagtaa aatgcaaatg ttcccaaatt tttattattt
      181 gttaaagcct gttctgtgca aagatgatgt ttataaaatt aataagataa cccatcaaga
      241 aaatagacaa aaaaacttga tagttcaaaa tttgaactat ggaacaaatc aatattctgt
      301 tgataattca aaatttgaac tatgcgacac atcaatgaaa acaagagaca aaaacaattt
      361 tgctgatagt tcaaaaaatg aactagaatt atataagtct tataaagata gtaaagatat
      421 taaagaatca cataatgatg atcttcttga atcactttca actaaaaata ataaattaga
      481 tgaagaattg atcgaaacat ttattgaaga aaatcttctt aaacaaatat ggggagaaag
      541 tattgtgaat tgcttaaaac tggctagcaa tagtgattat cgtcaattcg acaactggta
      601 caaaaaattc aaatatgcga ttagatctgc tgaaaaagaa caaaaaattc aactgaaaat
      661 tatctatgaa atttgtaata acaagtattt tgtagatcat gttagagaac agctatcaat
      721 gactttacgt gacttgatac gaagagcaaa aacagatcat cgtatcaaac aaaaagacga
      781 ttatatcttt gcttctctaa aaaataaagc gttagaacta attgaattgc aaatcagcga
      841 aggaatagat aagcaataag aatagaattg agtcaaatga aatggagggg tatctccact
      901 tcatttgaga actttgaata taggaggggt tattttgatt aaaaatcgtt taatagatca
      961 agtttggtgt ctagatcaaa atacagatag tctcgaatta atcgagacta tttgcttcaa
     1021 cactatagtt ctcgatttaa gagctgaaaa tgataattgt gtaactcatg tcataattaa
     1081 tcaaaaaatg gctcagaagc tctctgagag cctcagaaaa tgggctaagg gaggtaacga
     1141 tgaaaataat tgaaaaaaag attgccaata aaaaaataaa ttggttggtt gttaattttc
     1201 ttgttctgat agctatgatt atttttacaa gtaatattgc tcatgcgagt attttcaatt
     1261 ggtttgatga cacaaaaagt caaacagagt ttttgaataa ttctctttat agaccttacc
     1321 taaaaaagta caatggagat ttccttagtc agttcgggat ttggctcgga tgggctgtgg
     1381 taaaaggcat gtttactgtg acagattcga ttcaaaatat gattccagat gtgctggatt
     1441 tgttcaattt cattgaaagt acagggctaa ataatgttta tcaatcagcc gtaaatacaa
     1501 ttgtcattgg attaatgatc ctttcattga tgtttgttgg ttacaaaatg attactggaa
     1561 aaggaacagt ggatttaaaa tcggttggaa tgaacgtagt gatgtctata gcattaatac
     1621 ttttaatgcc aaccatgatt tctagtggta ttcaatttgc taaaacattt tataaggact
     1681 ctactacgat tacaaattca gatgacggag tcgcttggtc attgataaaa caaggagtaa
     1741 cagatttagc atatattaat aaaacagatc agtactcaag cattgataaa acggaggatc
     1801 gaaataattt aacacgaaaa aattttcaac agacggatct cacacaagtg ttaacggata
     1861 aagtgattga taaattggaa aaagaaaatc caattgcgga caatcttcgt tatgaattag
     1921 ttgaaaattc taataatgaa tttgttgcta caaagttttc tgacaacttc ttatcaacat
     1981 tttctgatag cttgaaatca ggctattacc gataccaagc aaatctatgg gggatttcaa
     2041 ttgggttgac agcgttagca atcgcctatg tgttcagcgc gtttgtgatc attacagcaa
     2101 tactagaact tgcatttaaa cgtgtgttag gtgttcttgt ttttgcaaca gatattgaga
     2161 caggacagcg ttcaaaagtt gttctatcag atattctaca atgttactta actgttgggt
     2221 ttcaaggatt tgggttatca atgtttgcta tgtttatcaa ttttctgaac tcaggacaag
     2281 gtatctcaac aaatattttt ataaaaacaa ttgcttatat ctgtgccgtt tttgttttaa
     2341 tcaaaggcag tggaacagtc atgagatact ttggtgttga tattggactc aaagaaggct
     2401 atgggcaact tgcttctgct tttggaatgg gcgcaatgct gtttagaaaa ggatcaaatg
     2461 gatttaatcg tgctaaagga agcggagaag gagaagatcg aaaaccagaa aagaatttcg
     2521 gcgaaactct atcaaaaaaa gctggcaaaa ctggaagagc tttgggatat gcccatgaac
     2581 gtggattatc cggtttagct tcagatggag ctacaatggc tagtgaaaga gcaacaaaac
     2641 catttaagag tatgcgtgat atagctaatg ataccaagaa taagtttaaa gaaggtttag
     2701 atgatggtac agtgtccgct attaataaaa atagcaaacc tatgttagct aaaaataaag
     2761 aagatgaaac tggaaaatat gctgattccg tgccaacacg tctttcagat agaaaagatg
     2821 gtgctaaagt tgaaaatgcc gatagaatta tgtctagttc tgaacgtatg cgtgaagcaa
     2881 tgaaaaataa tgctgaatcg aataataatc ctgcaagcgc aattcagcaa aaagtccaac
     2941 aagatattga agaacgtaaa aatgctatgc acggtcaagc taagtctgct gaagaattaa
     3001 ttaatcagaa gcgtcatgaa gcgaaataca cgcctgaagc aatgaatcgc gaggaaataa
     3061 tgttaaggaa acgtgtagaa gggcggacag gcgctaatat tgacggaaaa gaagcgttga
     3121 ccaaagaaag aattcaagaa gctaaaaatt caaatacagg attagaaaaa ttagtcaaag
     3181 aaaatccaca aaattctaca agtagtcatg gtggccgatc agtagatgtc cgagaaaata
     3241 ttcaaggttc actgaatggt catgttggac ggaatgttga tgtaagagag aatttacaaa
     3301 gttcttcaaa tgctcaaggt actaagacaa ttgatgttcg agaaaatctg caaagtagca
     3361 gtacaagtca accaaaaaca gtagatgttc gagaaaatgt acaaaattct acaagtagtc
     3421 aagggtttaa atcagtggat gtccgagaga atgtgcaagg tagcagtgca agtcaaccaa
     3481 aaacagtaga tgtccgagaa aatgtacaaa gttctacaag tagtcaagga ttcaaaacag
     3541 tagatgttcg ggagaacatt caaaaagata gtggagaaat gaaagaaaaa acacaaaaaa
     3601 tcaatatcgt tgaagacaga aagtcagcga aaaaatttga ttcaaatcat gaaacagtga
     3661 ttattgatag tgaaatccgt gaaaatgata agggatcaaa atcaagacga agattcactt
     3721 atgaagacaa tgagttattc agagatacac ttaatgatcc taatccactt ttcgataatt
     3781 tattaagaaa taaaaggggg ggattgt
//
LOCUS       DAIGOK010000174         3735 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.177, whole genome shotgun sequence.
ACCESSION   DAIGOK010000174 DAIGOK010000000
VERSION     DAIGOK010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.177"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(521..745)
                     /locus_tag="NOA01_002533"
     CDS             complement(521..745)
                     /locus_tag="NOA01_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633683.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            936..3713
                     /locus_tag="NOA01_002534"
     CDS             936..3713
                     /locus_tag="NOA01_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HCI0633684.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEVKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
BASE COUNT         1291 a          631 c          713 g         1100 t
ORIGIN      
        1 aatgtttata gatgaaactc tatttacttc ttttttaatt tgttcttgca atcttttttg
       61 ctacgtaatt tttgtaaaaa agtgggttga gagaaatcta attgattttc aaagaaggag
      121 tatgaaaatg agaaaaatat ttattcgttt attgtgggta ctatcttaga ggaataaata
      181 atagtggaga aaactatggt ttggcgcaga ttctatcagg tttgatggaa cgaagacaac
      241 ctcccgacat caagtcaaaa gctacttgag attttgatct gaaaataaaa aaagaaaaca
      301 aggtaggaac atcaatgttt ctaccttgtt ttcgtaatca atgggaaaaa actaagagtg
      361 gtgtacgtaa tcaataaaaa ggatggtgac aacagtacct ctttactctc tgaaagcatc
      421 ataactcgtt tttcctcatt atgccaataa attgttgtta taccacatat aaggatttgt
      481 aatagctaaa aacagacagg aaagttgaaa tctgtgaatc ttattttgta tggaaggatt
      541 tatagaacgc aaaaatcaag ataaagatga ataaaaatcc tgtttcggct atatattgcg
      601 tatctacgcc aataaaacga gtttgttctg tcatagccat ataaagcaaa ggcaaaaata
      661 caactacagc gagataagat ccgatgattt ctcgaaaagt tttccaggaa gtatgtttcc
      721 tgtttttata tttatccgat ttcattgaat agtcatagcc tccttattct ttcatatgtt
      781 gaaattccag tataaaaact ggaaggaact gtacgttttt tagaataaaa attataaggg
      841 ataagtaggc tgctgtctat aataagatat atttttcaat tctttattaa agattttatt
      901 gacaaaatga atccgttttc tatgatggag gaggggtgaa taagttgttg aagaaggaaa
      961 ttttatgtta cttagagaac cagacagcct ttttcgattt agaacatatg aacgaggtct
     1021 ttacagcaca aaggttagct gatcattttg gggtaaaaag aaacacagtc agtcattatt
     1081 tgaaccagtt aaatgatgag caactactag tcaaaatcaa ttctcgacca gtgatttatt
     1141 tccacaaaga agcttttgaa aaacaatttt ttaagttacg cacgaatttt tatttttcta
     1201 tcgatgatct caaagaggaa cagccttttt tcgcgcaacc aaaagactta ttctctttta
     1261 tgatcggata cgacgccagt ttgaaagaaa gtatcgaaca aatcaaaaca gctctttact
     1321 atccagatgg cggattacca cttttgatta caggcgaaag tggcacaggt aaaagttatt
     1381 tagttaattt ggtctatcag tattgtctgc ttcatgatct gctagaagat tctgcaccat
     1441 tcatcacttt taattgtgca caatatgcag ataatcctga acttttgact agcaatttgt
     1501 ttggacatgt taagggagcg tacactggtg cagaagaaaa taaaaaaggg gcttttgaag
     1561 cagcagatgg tgggatcttg tttttagacg aggtacatcg cttaacacca gaaggtcagg
     1621 aaaaactgtt cacctatctt gatcaaggtg tgatttatcg tatgggagaa cctaatttgt
     1681 cacgccggat caaaacacgt ctcttttttg ctacgactga agagattact agtcatttct
     1741 taacaacttt cattcgaaga atacctatcc gaatcgaact acctgcactt aatcagcgta
     1801 gtcgtaatga acggctagaa ctgatttact ctttttttct cgaagaacaa agaaaaatgg
     1861 ggagcccttt gtcagtcagt ggaccagctt tgtcactttt aacaagtcag aaattatccg
     1921 gaaacatagg agagctgaaa aatatcgtca aagtttcagc agcaaaagca tatgctgaac
     1981 agcgtgaaca gcctgaaatc catgtcacga tccatcattt gcaaaaagaa ctacttgcgc
     2041 agcctattac taaaaataca gcacagcaag gaatctatat tacgcaagac cagaccttag
     2101 agcaattaat ggagaagcaa cagcccgaac agcagcgaat cgttaaaagt tttgagcaaa
     2161 tcctaattga tttcaagaaa aataactgtc tgctatattc ttgcgaagta aaattaaagc
     2221 aagaagtgat tcaattattt gattttttgc tgtttgagac cagtagacaa gaaaaacacg
     2281 agctgctggt ttattggaca caatctatcc gagagacctt tcgacaaatg gaaactgcct
     2341 atcaaatcaa attcgatgga aacagtgtct atgccttgag ttactatctc tatcagcgaa
     2401 gcactatcaa atggttaccg gaagaacatg atattatcca actaatcaaa gaattagaac
     2461 aacaagtttc tcagtcttat ccatctagtt atcactatgt ccaacgaatt ctggaattaa
     2521 gtaagatcaa attagatatt gaagacacaa gtatggatcg gattttactg acgatctatc
     2581 taaaaaaagc gaaatggtca aaagaaaaac gactgccaaa agcgatgatt gctgctcacg
     2641 gttatgcaac tgctagcagt atggcaaatg tagtaaatcg tttattacaa gaggatctat
     2701 ttgaatcatt tgatatgcca ctagatgtca caccacagca gattgcttca gaaatccttg
     2761 attattgcga aaacagcgat gtttcaaatg gattgatcat attagtagac atgggatctc
     2821 tgaaagaaat ccatcagttt tttaaaaaac agttatccgt accgcttttg attttgaata
     2881 atgtgacgac acctttagca atcacagtag gggaatgcct gcaaaaaaat gtatcattag
     2941 aagagatagc agaagagact gttcgacaga tccagccaga atggcgactg ctctatccag
     3001 aggaaaacaa acccaaagca ctcattacta cttgcttcac tggaatcggt acggcggtcc
     3061 acttaagtga actgctagaa aaaagcttac ctacggcttg tcagttgaaa atcatcccat
     3121 atgaatatca gcaattaaaa gataagaaaa acagtgaccc attattttcc atatacgaag
     3181 tcgtaggaat gattggcacc acagatcctg atatcaccaa tatcgactat ctttcattag
     3241 aagacctcat ctcgggggaa aagatgtctg tactagctga atggttggac actactatga
     3301 atgcttcaga gaaggaaata ttcaatcagc gcatcattcg taatttttca ttggaaaaag
     3361 tactggattc cgtgactatt ctagatacag agaaggtgat gggagaaatc gatcatttta
     3421 tgagagaatt agaggtgcag ttgaatcgaa gcatcagcaa tcccaaaaaa ttagcgttgt
     3481 atgtccatgt gagctgtctc attgaacgat tgatccgaca aatgccaatc gaaacctatc
     3541 aaggattaga gaaatggaag cagtgtcaaa aagaagggtt atctgctatt aaatctgcgt
     3601 ttagtgtcat tgaagagcat tatagtgtca tgatccccaa ttctgagatt gcctatatct
     3661 atgatatttt gagtgaagat acagagtttc tctcgataga agaagaattt tgaacataag
     3721 caagttgttg ccact
//
LOCUS       DAIGOK010000175         3682 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.178, whole genome shotgun sequence.
ACCESSION   DAIGOK010000175 DAIGOK010000000
VERSION     DAIGOK010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.178"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(379..804)
                     /locus_tag="NOA01_002535"
     CDS             complement(379..804)
                     /locus_tag="NOA01_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633685.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            1178..2149
                     /locus_tag="NOA01_002536"
     CDS             1178..2149
                     /locus_tag="NOA01_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCI0633686.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            2181..3578
                     /locus_tag="NOA01_002537"
     CDS             2181..3578
                     /locus_tag="NOA01_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HCI0633687.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
BASE COUNT         1263 a          598 c          763 g         1058 t
ORIGIN      
        1 tctttgattt gtaaactgaa atgatccact gtttgtttta ctgatgaagc ttgatcaaag
       61 gaagaagctg agtttgaagt tcataaaaat cagccatagt gagttttgct tggatttgtt
      121 tatcttcttc tactttttac ataattttga acaggataag attcaaatgt tttaaatgaa
      181 tctttgacta gtttgtgaca gttgtcgcat atgctcaagc ttccttggtc attaaattct
      241 gtatggatat ttttgttgtt attacatgta caataatcag gactatcgat catccatttc
      301 gtctccttta caatcatctt gcttctttac tatagcagaa aagaagggga gaaacaaaag
      361 aagatccaac agacggcact atagactgta gatgttgcca tcctcatcct tcaaacggta
      421 attaccgatt tttgtaaata tgactgttgt ttctttttta ttttcttttc ctgaaataat
      481 atattcgtca tctttggtag tgatcttgat atctgtcagc actgtttttt cttcagatga
      541 ttcagaagaa tcagttcctt gtgtctgata cgtgcgtgtc cattccattt ttttatctgt
      601 aaatgtcaac tgtcccgcgg ataaatccat atctttacgt acttttagcg tatatacact
      661 gttggtggta tctgcttttt ccgcttttaa gacagaagaa tcggttgttt cgtccgagga
      721 ttggtcagag ccgccaaaca aatcacagcc agtcaatgtc aaaagtcccg cagacaaaat
      781 aaatagtacg attaatttct tcaaaatact tcccccattt gataggataa agaaattata
      841 ccattggaat gtaaaacttc aaacggatgg aagagaaaaa cgcacaaaac acccacaaat
      901 agagtaaatt ttagaataaa attttaaaaa ggcaaaaaat ccgccacttt cagaaagcgt
      961 tttaaaaaag tgttgcaatc ttttaaaaaa atgctatatt aaaaatgaac cggttcaaat
     1021 acttgtttaa gtataattaa tagtgagagg aagttgctct actgaagact ttagttatat
     1081 ggatattgat gacaagcaac actatgcaaa gaatgcggtc atcataatcg aacaacaaga
     1141 aagagaaatc aaatgtaact ggcaggagtg agaatgtatg gcatcgatcc gtgatgtggc
     1201 taagatggct ggtgtttcag tgggaacagt ttcccgttac ttgaatggtc agcaactgaa
     1261 ggaaaagaac atgaaaaaaa tagcagaagc gatttcagcg ttggactata aagaaaacat
     1321 cattgctaaa gggttgaaga ataatcgaag cttctctatt ggacttttga taaacgggat
     1381 ttccagccgt tttgggtcgg aagttgtctc agggatcgaa agagtagctg aagaaaatgg
     1441 gtatagctta ttattaagcg ggtttgccga tcagccagaa aaaattgaac aaaaaattga
     1501 atacctgatg aagcatgtga tcgatgggct gattgtcttc ttatccgaag aagagtggtc
     1561 tggatttgaa atgctcacaa aaatgagtat tccagttatc gcattgaatt gtccagaagg
     1621 accagcaggt attgatacaa tattagtgaa tgatcgagaa agtgtagcaa aagtgatcgc
     1681 tcatcatggt gaacaaggac ataaaaaaat cggatttatt gctgccactc aaacagacta
     1741 cgtagcaaga gaacgtttag caggagccaa agaagcggtc caaaatgatc catcgatcca
     1801 attagaggtc tttacaggag actattctag aaaaagcggc tattacgggg caaaagcatt
     1861 attagaaaaa ggggcaactg ctatttttgt cagcaattac aatatgtcga ttggtgccat
     1921 cgaatgtttc aatcaagaag gtgtcagaat cgggaaggat attgcgttca gtcactatga
     1981 ttatttagat aaaaacgatc tgagtatcct tccgaaaatc acgatcacgc caccaacaga
     2041 taaaatcggg gaacgagcag cagaacagct gttaaaacga atcaaaaaag aagaggaaga
     2101 aaatgaaaaa gtgattgtga tggaaaatat catacatgga atcaactaaa acgaactaaa
     2161 aaagaaaaag aggtgctttg atgaaaaaat atcaatttcc agcaaatttt tggtggggtt
     2221 ctgcagcaag tggcccacaa acagaaggaa ggatttcagg cgatggaaaa ggagagaaca
     2281 tttttgatta ttggtacaaa aaagaaccgc aaaagttctt tgatcaagta ggacctgaaa
     2341 aaacctcgca agtttatacg aagtatcaag aggatgtaca gttgatgaaa cagactgggc
     2401 ataattcatt tcgaacatct atccaatgga gccgtttgat acctgatgaa tctggaacaa
     2461 tcaacgaaga agccgttcgt ttctatcatc gttattttga tgagttgatt gaaaacggta
     2521 tcgaaccctt tgtgaatctg tatcatttcg atatgccgat gtatctgcaa gaaaaaggcg
     2581 gatggttgaa ccgagaaacg gttgaagcct atgaaaaata tgccaaaagt tgttttgaat
     2641 tgtttggttc gaaagtaaaa aaatggttta ctcataatga accaatcgtt ccagtcgaag
     2701 caggttattt atatcgatgg cattatccag aagagtcaga tatgaaaaaa gcgatccaag
     2761 ttggctatca tgaagcgttg gcttcagcgt tagccatcaa atcttaccat gaaatggagc
     2821 aaggtggtgt tattggtatc atcttgaatc tgacaccaag ctatccaaga gacgaatcaa
     2881 accctgaaga tgttaaagca gcgcaaatag cagatgcgtt cttcaatcgt tcattccttg
     2941 atccagctgt aaaaggcgag tttccaagtg aattgattga gatagtaaaa gaacttgatc
     3001 tcatgcctgt gatccatgca gaggatttga cggttatcaa agaaaataca gtagatcttt
     3061 taggtatcaa ttattaccag ccacgccggg taaaagcaaa agaaacacca atcgatacta
     3121 aaaacggtcc aatgcctgag gattatttcg acaattatga aatgcctggt cgtaaaatga
     3181 atccttatcg cggatgggag atctatgaac aaggaatcta tgatattttg atcaatgtac
     3241 gagatcatta tggaaatatc gactgcttta tttctgaaaa cggtatgggt gtagaaaacg
     3301 aagcacgatt tatcaaagaa gatggcatga tccacgatga ttatcggatc gaatttgtcc
     3361 aatctcatct gaaatatgtc catcaagcca ttcaagaagg tgccaattgt ttaggctatc
     3421 atatgtggac atgtatggat aactggtcgt ggaccaatgc atataagaat cgatatggct
     3481 ttatttcagt agacttagca caagatggaa aaagaacaat caaaaaatcg ggtcattggt
     3541 tcaaagacgt atcagataaa aacggatttg aggcttaaga aagaatagtt gagagcaaat
     3601 gcgtgaaatt ctccattctt accattttgt gaaaaagaag ttcaattatt tacgatcttt
     3661 ttcaactttt ttgtctttct at
//
LOCUS       DAIGOK010000176         3601 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.179, whole genome shotgun sequence.
ACCESSION   DAIGOK010000176 DAIGOK010000000
VERSION     DAIGOK010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.179"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(<1..709)
                     /locus_tag="NOA01_002538"
     CDS             complement(<1..709)
                     /locus_tag="NOA01_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCI0633688.1"
                     /translation="MTYTHLTTDELVMIESYFKINQSVAKTAHFLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIR
                     CSARTIYRMFKKGLFNPSDLPMKGKRKPNGHQERRGKQAFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIENKLNHWFESVPKNLFK
                     SITFDCGKEFSNWKQISN"
     gene            975..1319
                     /locus_tag="NOA01_002539"
     CDS             975..1319
                     /locus_tag="NOA01_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCI0633689.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            1320..1739
                     /locus_tag="NOA01_002540"
                     /pseudo
     CDS             1320..1739
                     /locus_tag="NOA01_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            complement(1831..2181)
                     /locus_tag="NOA01_002541"
     CDS             complement(1831..2181)
                     /locus_tag="NOA01_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633690.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(2147..3478)
                     /locus_tag="NOA01_002542"
     CDS             complement(2147..3478)
                     /locus_tag="NOA01_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HCI0633691.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAAFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
BASE COUNT         1268 a          650 c          677 g         1006 t
ORIGIN      
        1 cattactgat ctgtttccaa tttgaaaatt cctttccaca atcaaaagtg atggatttga
       61 atagattttt cggtacagat tcaaaccaat gatttaattt gttttcaata tcaatcgctt
      121 gtctaccaca cggtttcaat gtgatgataa cttttgataa tcgctcaact aaggtaatta
      181 cagcactttt atgtttcaaa cctacgatag tgtcaccttc aaggtgacca aactcatttg
      241 agaattggct ataatctttt tcacgttcat gaatagagcg acggaaagct tgttttccac
      301 gtctttcttg atgtccattt ggtttacgct tgcctttcat cggtaagtca gaaggattaa
      361 atagcccctt tttgaacata cgataaatgg tacgagcaga acaacgaata ggaaacgctg
      421 cacgaccaac aatcacatcg ggtgtccatc cttgaacaac ctttctttga atatattctg
      481 attgttcctc aggtaaaaca agcgggcgtc taccacagtt tgatttgttt ttcttatact
      541 gttgatagta ctctaaggct gattttcctt gcttgaagaa caggtatact ttatggatcg
      601 tttgtcttga acgattcaag aaatgcgcag ttttcgcaac agattgattt attttgaaat
      661 aagactctat cattacaagt tcatccgttg taagatgggt ataggtcatt tgtactcact
      721 ccttataatt ctttggacgg aactattttg agtgtagcac aaatgacttt tttagttgtc
      781 tagcttaatt ttacaatcgg cgaataaaaa aagcgttagc aactatgctt aaaaagcata
      841 tcgactgaat aaactaagca ttggacaggc tgaaagacta catttaaaat gaaatgcgaa
      901 gaaacaatag aaaataaagt tgccttagaa ccatagttga atcacctaac agtcgtgaag
      961 ggagaataag agaaatggca gaaaaacaaa ccgcaggacg agatcaatta ggagattttg
     1021 caccaaaatt tgcagaattg aatgatgatg ttttatttgg acaagtatgg tccagagaag
     1081 aattatctgc tcatcagcgt agtatcatca ccatatctgc attaatttca ggaggaaatt
     1141 ttgaacaatt gccagctcat ctgaagattg gcaagcaaaa tggcattact agtgaagaaa
     1201 tagcagaaat cattacccat ttagcttttt atgtaggatg gccaaaagct tggtcagctt
     1261 ttagtttagc aaaagaaatc tatttagaag aaaatagcga agataagaaa gaggcgtaaa
     1321 tgacgacaaa acacgaagaa atacacaatg gcgtgatttt tccagcaggt gaaaaaaacg
     1381 aagcttacgc tcaagtattt accgggtaaa gctatctgaa tatgttagtg aatgatcccg
     1441 aagtttctgt tggtgtaggt aatgtaactt ttgaaccagg ttgtcgcaat cactggcatg
     1501 tccataaaga cggctatcaa atcttacttg tgaccggagg agaaggctgg tatcaggaag
     1561 aaggaaagcc agcaagacga ttaatggctg gagatgtcgt cgtaacgcac aaagggatta
     1621 aacattggca tggtgcgaca aaagacagtt ggtttgaaca tttggcgatc actgctggta
     1681 attcagagtt tttagaacct gtaacagatg aagtgtatga aggattggaa aatcaataat
     1741 gtcaagcttg aacagctcct gaaagtagat tttaatcgaa ctttcaggag ctgctttttt
     1801 gattcagtct tttataaata gaaaataggg ttaataatag gaatgagaag cacgtcgaaa
     1861 gatcttaata ttgataatcg gctgggcata catgatttcc acgtcatcaa tcaaactacc
     1921 taacagatga taatcttccc gatcatgtcg gggattacct aatagttctt gataatagat
     1981 ttccatacta gggtcccgag gattgttcag caaatcagca agtacatcta aatcttctgg
     2041 ctgagtttct gccgtaccct gtatctctat gaaaagccct tcctcagcaa attctactcc
     2101 aatacgtatt tcagttattt catgggaata taaataagta agaagctcat cgatgattct
     2161 ttttgataat tctttttcca agtgtctttt tttcctttct gacagcggta aagttatcta
     2221 gtatcggtac aaggatacct gcaacgaaac cagttgaaaa accactgtta tatagattta
     2281 gtcctccgtg caaagtgctg acgttatgga caagagcaat atgtagtaca ccagctatta
     2341 caccgtaaaa actgccgtaa aaaccgctta tgggcgcgag acctgtccca aacacagctg
     2401 caacaagcat gccagtactg gtgatctcat gaaaagttcc aaacaaagaa gcaataaata
     2461 ttccgactag tatcggaaaa ctgtttttta aatgacaacc aaaagcactg aaacccaccg
     2521 cagataaaat agcaccgatg acaggtccat ttaactgacc gccaaccagc aaaacataag
     2581 aagacagcat cagaccaact aagcctatgt tgatcaaagt agcaccaata ccagaattag
     2641 caatgaaatc agttgtcaat tttccagatg aatcgaaaat ttctcgaatc cccaaaagcc
     2701 gaaaggaatt ataatagaaa ccaattccga ctgtcagcga aaaaaagcca aaaataatcc
     2761 aaatgagagg aaagtgatat tcattgaaaa ccaatgtatt ttcgacgatt gaatagctga
     2821 ataacctcaa aacagcagta aaagtcatcg caatcaaccc agaggtgaat ccaatattat
     2881 aaagtgtgaa tccacgatgg aaaactaaag tttgagcagc taagggcgga agaagtagac
     2941 caattgctat gccagctaaa ttccccaata aaatacctat cagtaaagga aaacgcatac
     3001 caaaagtgat ataactaacg accggtgata atgcgctacc aaacagtccg atcatgatgt
     3061 actgagaaaa aggtttattg acaaatgcag cgtatagata cacacctaaa atgattgaga
     3121 tagaattata aaggtttttg ccaaaaaaag agaaaccggt aactgttaga atcgaagcaa
     3181 ataagggacc gtttaaaatc accttgtgcc gataagctaa aagcacagaa agtaaggtca
     3241 aaatccctac atttatgaat gcgcttccga aaccgcctaa agcaaaataa tctgtcaaaa
     3301 gattgcttgg cgaggtaaga atagttagca atccactcca attatcagta ggagtattag
     3361 aaagcaagcc aataaaaaga aaaaaaacac ctgtaaaacc aagataaaaa tactttcttt
     3421 tctgtgaaac gattcgtttt tgaggatgaa tataaataac gtcagtttcg ttttccatta
     3481 cacaccttct ttttcatttt ctttaactat aacttgaaat gaaagatttg gacaactatt
     3541 atttaggtca aaactaaaaa tgtaagggaa cccattgaat ttaaccaaat ttttttaatt
     3601 t
//
LOCUS       DAIGOK010000177         3486 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.180, whole genome shotgun sequence.
ACCESSION   DAIGOK010000177 DAIGOK010000000
VERSION     DAIGOK010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.180"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..612
                     /locus_tag="NOA01_002543"
     CDS             <1..612
                     /locus_tag="NOA01_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633692.1"
                     /translation="RPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVASKESV
                     DNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQYQFKD
                     KHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTTVSSQ
                     KNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            1361..3454
                     /locus_tag="NOA01_002544"
     CDS             1361..3454
                     /locus_tag="NOA01_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HCI0633693.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT         1168 a          616 c          698 g         1004 t
ORIGIN      
        1 agaccgtttc ttctcttttg gttattcggt tttttcttac tgcttatttt atccgcagca
       61 ttttcaggaa aaatgacagc agatcttgct cagaaagaaa cagaaaaaat cgtagcaagc
      121 aaagaatcag tagataattt caaaaaagaa ttctcaactt attggtgggc ggagtataaa
      181 ataactgcca attacgaaaa aggcgaattg gacatttatt tgccaaaagg aaaagaagaa
      241 aatcaattta ttaatatcga tgacgtactt agtgcgattt ctctcagaca atatcaattt
      301 aaagacaagc atttgaccgt tttgcttttg actcatgatg gcgacaaagt agcccgtttg
      361 aaaaatggaa aagtgatcac cgcttattct gaaaaagtag acaaaaaagt ggtcgccgat
      421 cgtttagcgg aatggaaaaa gacaaatgaa caagtcaaga aaacaactgt ctcttctcaa
      481 aaaaacgagt cgacaggaac aagtccttca gattctggca gtggacaaac gataacggga
      541 agtgaagaaa atcaagcctc aattattacg aatgagtcat cacaaggaca atatcaatcc
      601 tatggacaat gaaaaacaga ggttgtgaaa tcagcgtttt gacctgagta ttagagaaac
      661 aaacggcaaa atagttcctc atattttgcc gttttttgag ctaataccga aggtcagctg
      721 attgaacacc gtgtatcaag aggtcttgag cctgccgttt aactctgagc cacaaggagc
      781 aatttcaaaa aatagctcct tatatttttc gtttggttgg gcttattacc gcagatgcaa
      841 gcttttgaac accgtttacc aagaggttgt aaaattagcg tcctgacata atctaatacc
      901 gagggtcagc tgattgaaca ccgtgtatca agaggtcttg agcctgccgt ttaactctga
      961 gccacaagga acaatttcaa aaaacagctt ctcatgtttt ataaatattg tgacttgtgg
     1021 ccgaggagtt ggcaggagaa caccgtttaa ggaagaagtt gtgaaaaagc tgtcctgcat
     1081 caagaaataa ggagctgcga caagaatttt gtcacagcct ctttttttgt acgaataccg
     1141 agaaactggc ttttgaatac cgtggagtga agcggtcacc acaagacttt tatctcttca
     1201 ttagtttaat cgcttacatc gcagcggttt tatgctgtga aaagtggacg gaataatctt
     1261 tagaaagaat aaaaaaatct taagataaca acttttttag gaaattttca tttttcagga
     1321 gcaaaataag aaaaaatgtg aatagagaga agtgatatac gtgacacaac ttaaaaaatt
     1381 gtgccaaaat cgtttagcaa tcgttttgac agctgttttc tttttctggc tgaagacgat
     1441 cattgcctac tatgcagatt tttcattagg tgtggaaggt acgatccagt actttatctt
     1501 gtggatcaac ccaattgcga ctaccttatt gttttttggc ctttctttgt atattaaaaa
     1561 gccaaagcca gcgttagcta ttcttttgat cattgatatt ttaaatacgc ttttgttgta
     1621 cttgaatatt attttttatc gggaattcac agatttcatt acagtcaaat ctgtattagg
     1681 cttttcgaaa gtgtcgcaag gattgtcagg aagctccttt tctttgatga agccccatga
     1741 tgtcatctac tggctggata tcgctgtgtt cattgggcta ctggtttggt tgaaggtcaa
     1801 gaaaatacct atcaaaagca accctgtcgg caaaccgatg gctatcgctg ttacctgtct
     1861 ttcagggctt attttttcag gaaatctagc gctgagcgaa gccaaccgtc cacaattgct
     1921 gcagcgaaca tttgaccgtt catatattgt caaatatcta ggaatcgatg cgtatactat
     1981 ttatgacgga atcaaaacag gaatgactag cagtgttcgc gcgcatgcaa gcagtaatgg
     2041 gattgatgaa gttttagatt atacaaagaa acattatgca gagccaaatc ccgaaacatt
     2101 tgggatcgct aaagggaaaa atgtgatcgt tctccattta gaaagtttcc agcagttttt
     2161 gattaatatg aaggtagacg gacaagaagt cacaccgttt ttaaatagta ttttccagaa
     2221 tcaagcaacg atcagctttg acaatttctt tcatgaagta ggacagggaa agacaagtga
     2281 tgctgaaaat atgttggaaa ctggcacatt tggtttacct caaggctcat tattcacaga
     2341 acttgggtca gacaatgtct ttcaagccgc accagcgatc cttggacaaa agcaaggcta
     2401 tacaagcgct gttttccatg ggaatgtagc tagtttttgg aatagagatc atgtatataa
     2461 aaacttaggt tatgacaatt tctttgatcg atcttatttt gatgaatccg acgaaacgtt
     2521 aggttatggg atattagata aagatttatt cagagaatct gcccaatatc ttgagcatct
     2581 acaacagccg ttttacacga aatttctttc cgtaaccaac cacacgcctt attatacgga
     2641 tgacaagaat ttcgactttc catctttgaa tactggcaat agcacagtag ataattatgt
     2701 tcgaacggca cattatctgg atcaatcatt ggaacaattt tttacgtatt tgaaaaaatc
     2761 tggtatttac caaaattcaa tctttgtgat ttacggggat cattttggga tctcgaatac
     2821 agataataaa gatttagcaa gcgctcttgg taaagatcca gatacatggg atgaatttga
     2881 caacgcacag atgcaacgag tgccattgat gatccatatg cctggttata ctaaagggac
     2941 agtcaatcat gaatacggtg gagaaatcga tgttttgccg acattactac atctattggg
     3001 aatcgatgat aaagattatc ttcattttgg tacagactta ttatcttctc agcatgatca
     3061 agtagttgct tttcgaaatg ggaactttgt gacacccaaa tacaccgtcc taggaggaaa
     3121 agcttacaat aatcagacag gagaaatcat cgatacgaaa gcagcaggaa tcgatgagga
     3181 aatctccaaa gatcaggaaa aagtcaagac tgcgttaagt ctttcagata agctgaatca
     3241 ggaaaatctc ttgcggtttt atgtgccaaa cggatttgag acgattgatc cgaaaaaata
     3301 tgactataaa aatgaagcgg aacgtaatga agcaatcgaa aagcaaaaag gcgaacattc
     3361 gacaagtgtc ttttcaaaaa atggtaataa aacaacaacc aacttatatc cagtaacaag
     3421 aacagacgtt gaacagcaaa aaaataatca ataaacaatc aaaccaacct tcagtagtta
     3481 gacaac
//
LOCUS       DAIGOK010000178         3483 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.181, whole genome shotgun sequence.
ACCESSION   DAIGOK010000178 DAIGOK010000000
VERSION     DAIGOK010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3483
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.181"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(<1..2232)
                     /locus_tag="NOA01_002545"
     CDS             complement(<1..2232)
                     /locus_tag="NOA01_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633694.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTG"
     gene            complement(2904..3172)
                     /locus_tag="NOA01_002546"
                     /pseudo
     CDS             complement(2904..3172)
                     /locus_tag="NOA01_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT          978 a          771 c          549 g         1185 t
ORIGIN      
        1 accggtttgt agcgtttctc cttctcggtt ctgtaactca aaaacagctc cttgaaggac
       61 ttcaccactg ccttcagcat gtttaattag ttcaacacca cccgtatcaa ggatgttttc
      121 aatggttaac gtcacttctt ctatggagta ttcatttata aaaacatcat gtaccgtcgc
      181 atctaaaaga tacccttccg gtgcttctgt ttctcggact tgatagctac ctacaggaag
      241 cggttcactt aaaaattctc cgttttctgc cgttatacct tcttgtatca cttgtgaatc
      301 gcttgataaa atttcaaatt ttgctcctgc cagcctttgg ccattctcat cagttttaat
      361 tactttaata cgcccatcga ttttccttgt tcttacagta ctcgtattca atcgattgtc
      421 actattcaca agcgttgcaa agtttaaaat ttgatcgccg accgcatacg cctctacatt
      481 gaccttaaaa gagagggtaa aacttgtcgg accaccaatc gtattattcg cactgaaacg
      541 aactattcca ttattgactt cggcatcaat gaaagaggga atatctgata gaaatgacac
      601 aaattccatg ccttcttcca aaacatcttc aataacaagc gtatttcttg ttccagaaaa
      661 catcgagaca tgcagagtat acgttagttc attttcattg agattgataa catttttgtc
      721 cacacttttt cttaaagaga ttgccggaag attactttca gggattctca agcggtagtc
      781 tcggctcatt acctgattat tgtaggtcat actcgctcga ttccgagaaa acccatcctc
      841 ttgtcttcct tcattgaaca agtcgacatc ggtcacttca agagcatatt caataaaata
      901 cgattgaccc tctgctagat taccaaagtc cacaacaagt gtattcgttt ccttattaaa
      961 agatactttg tccacaaatt cctcggaaac ataatgagtt ctaacgtttg cttcacctgg
     1021 attcgttact tctgcgatcc gaaccccacc tgaaataggt tctctatttc cagtggaact
     1081 ccatgtagtc ggattcggtg ccaaagttgt tccttgcggt agtacatcgg taaccactac
     1141 accgtctttt cgtgatcctt cataatttac ctgcaaaata aatttattac tgttggtcac
     1201 gtcttgcccg tcaatcagca gattcccaag atttccttgg ctgtcgattt ctctagagcc
     1261 atagtataat ttcgaaaaaa tgggtcttga tgaaggaata ggcgtaattg aaaattccgt
     1321 gtttgctgct cgctcagcat aagtaagact cacttgaaag tcattatttg gctcagtacc
     1381 gcctgattga aagaaagcaa cttcaaacgt gatactgaat gtatacgact gagcggcttc
     1441 atttaatcct tcataatcaa aggtaaatgg aaaaacaacg tctgtgcctt cttggtaaaa
     1501 tgccagtggc tttaaaggtg aatcatcagc gacctccact ctaatgaact caattaagcc
     1561 aatcgatgct ctaggaactc gtacgaccat ctccccatat tcttcaagaa tatcgccagc
     1621 gcctccttga atgtatccct ttaacgtttg tgtatgcctg ctcggcaaag tggggcgctc
     1681 agcagagatg ccgatgtcat aattcaagag ctcctcttgt ctttcaatct gagattcctg
     1741 cgttatagat ggctcagatg atctggcttc attaaaagat tcacttaagc tttcttctgg
     1801 tatattaaaa gattcacttg tgctttcttc tggtacatta aaagactcac ttgtgctttc
     1861 ttctggtaca ctgatttcta ttgagaccgt atttgattga atatcaccgc ttcttgcggt
     1921 caattcgtat ttacctgaat gctgggaaga ataagtaatc agacgttcgt aatcattgag
     1981 ttgcttcact tccccttcaa tcaaatcctc actcttatca atgaccaatg catttagcag
     2041 gtctggtgtt cctctgactc gcaactggaa ctgctcaccc aaagcagcct cctcttgatc
     2101 aacttcgatc gtcagcgaac gctctggctg ttctgctaaa gactcagcta gaaccgttat
     2161 gttcccaaat tgattgatca caataagcat aactagaaac atacttaaca gtttatgaaa
     2221 tttgttcctc actattactc ccccttataa ttttgactaa acaacaaggt acccgataat
     2281 tttctaaaaa gattccccaa ctcaaaatgg ctgcgttacc tccctcgtat tttcggttca
     2341 actcaagcgt tgcaatgtaa gctgtaaaca aagctaatga ttgccgattg tcttctgttg
     2401 attgattcga tagctttgta tgacttttag ttgtccgtgt ggcttaacca taattatgat
     2461 ttcacgattc ctcctgccag tgccttgcac aagctggagc gtaaccctaa tagttatttt
     2521 gttaatgtac ttatatactt ttatactttt ccattagaaa caatcactaa atcgttgata
     2581 ccaaggtcag actctagaat ttcatcagtg agtccctgta ttggttaacc ttcatcaatc
     2641 ccaaaaaaat atatcaatgc ttagcatcaa aatgaatata tagtttagaa tacttgggat
     2701 tcatcgataa ctgatcgctt atttggccat attatttggc acttggttca actaaacgca
     2761 tttaggatgt tccttcattc tcgtcatgga tgttctcaaa ccgtcattac taataaactg
     2821 tccaccgaat cgatggttca tttcttgcat tttccacgtc caattactct ttttgctctt
     2881 tatttgggcg tagtttcact ttcttaactt gtatcgtctt ccatcaactc cttaatcatc
     2941 ttcttagctt tattggctcg gtttccttgt aaaaggtatc tacaaatagt gacaatctgt
     3001 attaaatctt catcattttc tgtaatgtca ataatttcta tattctgtac catatattgc
     3061 aaagggtttc actaagttca taaccgaagc gaattaaaca acttttgtat agaataacta
     3121 ccttttctat ttttccattg gtaattctat ctattaattg gtttaatcct tttgttgtag
     3181 ttgattccac taccaagatg attctctcga attttaaagc ttgaagatgt attgctattt
     3241 ttgctaactg gcgtatttgc aactattttt cccttctatt cttccttcat tgattcccct
     3301 ctaaaattac tatccataaa aaattgcata aatgacaata gtatttatat tagtcatact
     3361 gctttttaac acagcaaatt ccagaaaaac attcgttaca tatttgtgaa tttttccaaa
     3421 aaataacatt tttataaaac gacgaaaaat aaatcactac cagtcaactt ctataatatt
     3481 acc
//
LOCUS       DAIGOK010000179         3460 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.182, whole genome shotgun sequence.
ACCESSION   DAIGOK010000179 DAIGOK010000000
VERSION     DAIGOK010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.182"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(<1..182)
                     /locus_tag="NOA01_002547"
     CDS             complement(<1..182)
                     /locus_tag="NOA01_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCI0633695.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
     gene            517..951
                     /locus_tag="NOA01_002548"
     CDS             517..951
                     /locus_tag="NOA01_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCI0633696.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            974..1294
                     /locus_tag="NOA01_002549"
     CDS             974..1294
                     /locus_tag="NOA01_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633697.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            1435..1626
                     /locus_tag="NOA01_002550"
     CDS             1435..1626
                     /locus_tag="NOA01_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633698.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(1697..1954)
                     /locus_tag="NOA01_002551"
     CDS             complement(1697..1954)
                     /locus_tag="NOA01_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HCI0633699.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            2112..2375
                     /locus_tag="NOA01_002552"
     CDS             2112..2375
                     /locus_tag="NOA01_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633700.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            2399..2587
                     /locus_tag="NOA01_002553"
     CDS             2399..2587
                     /locus_tag="NOA01_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633701.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(2670..2855)
                     /locus_tag="NOA01_002554"
     CDS             complement(2670..2855)
                     /locus_tag="NOA01_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633702.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            3070..3441
                     /locus_tag="NOA01_002555"
     CDS             3070..3441
                     /locus_tag="NOA01_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCI0633703.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
BASE COUNT         1293 a          493 c          697 g          977 t
ORIGIN      
        1 gaatttgatt ctgcaatggc attgatcaag gagttatcac caacgtattt ttgagaaatc
       61 gttgatagag tatcacctga ttgaacggta taagcttcgt ccgcatgagc agtcgttccc
      121 atgaagaatg cagcgccggc agccaaagtt gttccaaaaa gtaaagtttt aagtgatgtc
      181 atgaaaaatc tcctttattc gttaaaaagt tttatttgtt gtgttcaacg aggaatactt
      241 taacacgtga aaatatttca taggtaaaag aagcatgaca atttatgaca aatccacgtt
      301 ttttgtaaca gaatgatgtt cgctcgatgt gaaaaatgat gaggaatttc acataatgca
      361 gaaataatgg gattagaaca ggtgttctga tgagaaaaga aatgtataaa taacttgaaa
      421 gaaattgctt ttgttcatca ttttatttac atattgcgta tattttttgt atttttgctt
      481 gagatgttac aataaaagaa aaaggaggcg gttccaatga gtaaaagata ccagaacatc
      541 atggtagcag ttgacggatc aagacaatcg atcaaagcgt tcgctgaagc ccttgactta
      601 gcaaaggaca atgaagccca cttatttatc gtgtcaatca tcaacaaagt tgagctgacc
      661 catagtgcct atgccttttc aaaaatctat gcagatgaaa aacaaagaac agaagtagcc
      721 atgctgaaaa aaatcaatga tgccaaagag tacggcattg ctgagatcca cgcaatagtt
      781 gaaactggag atccaagaaa tttgattgcc aatgtcatcc ctggacaaga aaatattgat
      841 ttgatcgtta tgggagcgac aggaaaaggt gctatacaac aagcattagt tggatcgacc
      901 gcttcttatg tcgtatcaca tgccccttgc agtgtgttgg tcgtgaagta atgagataag
      961 ggaggaatag aaaatggttc gtcctcaaga agtccgtgcg ccaaaagaaa aaatagaaat
     1021 tttagcaatt attgaagacg gcacacaaac aaagaaggga tactcgatcg ctttagtaaa
     1081 gtggaaagga aaaaaaggtg ttgctctgag atgggatgga gataaccaac aagataaagg
     1141 attccctgtc acagctaacg gttatcatcc agcgtggttt gttctgcctg ataaatttat
     1201 ggaattatac agctatgatt atgcaagagc cgcagcatca atcaaagcat tagataaatt
     1261 aacagaagag cagaatgaaa tggaggaaaa ataaaaaaga cgaaccaatc agcctttaga
     1321 aaactgatta gttcgaggag taaaaatgaa aaaaggttat ttgggatgaa actagaataa
     1381 tgcgtataac ttaaataaaa cttaaagcaa taaaatggca agagggtgag gcaaatggaa
     1441 gcaggaaaag tattacagga agtaaaagca ctatgtgcag ataaaaaata tgaagaagcg
     1501 aaaagcctga tcgaaaagaa caaagaaaat ttggaagaaa agtacccgat cgcacaagaa
     1561 tttatagaat taaaacaagg aacgatcctt caccgattca aacatttttt tggtataaca
     1621 gaatagaaaa aacgattaaa gatcggcaga aatttgctgc atctttaatc gttttctttt
     1681 taaacatgcg agggattcaa ttctgattct tatataaaaa atagatccaa ataataagaa
     1741 aaattactga aagcgtcaat tcgatgatgt gtgttattaa tacagacgtt gtttcccaat
     1801 gcctaaaaga aaaaatttga taagtaaaat atacagcagg taaagaggcc agtaaaactg
     1861 aaaacttctt ttttgtccat tttatgtaat agaaaagaat gcttatcata ataaaaaaag
     1921 gaatataaaa aacaaattga ccgatcgcga tcatactatt gcctccgata aataaagtcc
     1981 atgatacaaa ataacacttt gtgcgaaatg gcgcaagcag ttttgtggat ttatccggta
     2041 aaatctgtat cgattttgaa attagaggag aaacttgcta taatcaaagt gaaagaagga
     2101 gtgggatttt catggatcat gtagtgttag aaatcgattt gcatttgatc aataatattc
     2161 gggtgattta ctatgttgta agtaacagtg tcgaacaacg tgtactgacg aataaaataa
     2221 atggtatttt agcaaagaaa gacgttcacc gattcaataa tggtgaagga agttattata
     2281 gtatcccagt agaaaaaatt atttatacga cagtcaaagt cagagaagat ctagatacca
     2341 aaaaagcata tgaacctatt tttacaactt attgattgac agcagatgga ggaaaaaaat
     2401 gaatccatat gaacaactta ttcaagattt gatcgatgga aaaatcgaaa agattgatgt
     2461 aaaaagagaa gaattaatgg cttttcgaga agcgtggctg aagttggaga atcgtaaata
     2521 tgtggtggga aaagcctcac tgaacggcaa tgtgacttat cattacgatc cgacacgttt
     2581 attttagtat gaagtattca gaaaagaacc aaaggaggac gatgcagcag cattgtcctc
     2641 ctttgatttt acaaaagtga aagttttact tatttgaata gatttttaaa tttgtttttg
     2701 acactgtcga tatttacatc ttttaaaaga ttttgggctt tctctttata aggtcctaat
     2761 tgttctttat gttcttcgat aaatcgtttt gcgccatcga aattgccgtt cttaatcatt
     2821 tccttgactt tttgaaataa ttcttcttga ttcattttga acacatcctt tgctatcaac
     2881 atgatttttt acatggacaa aacgagaaaa actgatttat ccacacgtca ataatatcaa
     2941 ataacgatca tttttactaa aaaaacactc gattctcgct tctaaagtag gagaaaaatc
     3001 tccaacttgg gacggatgga atttctttaa ctgacccata tactgaatat ataacaagga
     3061 ggtagaaaga tgaaagtgat taaatatttg attggactgg cggcgattag tggtattttg
     3121 ctatttggac tgaaatatta tactgaagga agttatggag aaatccctgg tatacttgat
     3181 cagctaaatc cattagttga aaaaggagag gtctatgtaa agacacaaaa accagaggaa
     3241 gtaaatgaat atggtacagc aaggtatgtg cagaaagctg ctgatgccaa tgggaaagaa
     3301 cgaacggttg aatttaatgg tctttctgtg ttaaaagaaa atcattattt gaaaattagc
     3361 aataaaggag cacacataga aacgtatgag gaagttccta gagaaaaagt tccggagaaa
     3421 gcattagcaa ttattgggta aatcagtttt tgtattaaga
//
LOCUS       DAIGOK010000180         3425 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.183, whole genome shotgun sequence.
ACCESSION   DAIGOK010000180 DAIGOK010000000
VERSION     DAIGOK010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.183"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            36..926
                     /locus_tag="NOA01_002556"
     CDS             36..926
                     /locus_tag="NOA01_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCI0633704.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(999..1823)
                     /gene="nadE"
                     /locus_tag="NOA01_002557"
     CDS             complement(999..1823)
                     /gene="nadE"
                     /locus_tag="NOA01_002557"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCI0633705.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(1835..3307)
                     /locus_tag="NOA01_002558"
     CDS             complement(1835..3307)
                     /locus_tag="NOA01_002558"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCI0633706.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
BASE COUNT          971 a          718 c          618 g         1118 t
ORIGIN      
        1 aatatataag aaaaaccttt taagaggtga tatatatgca actacaaata aacgaagaat
       61 ccttaccggt atatgaagca ttagctagta aaacaaggat caagatcatc cagctacttt
      121 ccaaaaaaaa gatgaatgtc aaagatttgg caaaagaact tggagtgagc agtgctatca
      181 cgacgatgca cgtgaaaaag ctagaggaag caaacatcat caaaacagaa aaagtaggac
      241 aacaaaaaat ttcgagcctt cgtgtcgata aaatcgatat cagttttcca gaaaaaatat
      301 tcaatgcttt tgatacgaaa gaaacatcga tacctattgg tcactataca aattatgcaa
      361 ttgaaccaac atgcggttta gctacgatcc atgattttat cgggaaagtg gatgaaccac
      421 gctattttat ggatcctcga aggatggatg cccgcattct ctggtttacc agcggatttg
      481 tcgagtatca ggcacctaat tttttaaata cagaggatac attggagatg ttggaagttt
      541 ctgtggaaat ttcttctgaa tttccttttt caaatgacaa ttggccttca gatatcactt
      601 tcagcctaaa tggtgtggag ctaggaacat ggacgagccc tggagatttt gctgatatcc
      661 gcggtaaata tacgcctgat tggtatccag ataatcttaa tcaatacggc ttgctgaaga
      721 cgatccgaat taccaaacac ttgacgaata tgaatggaga acccttgtca aacatcacga
      781 tcaacgacat ccctaaagag caagacactt ggcatttgcg aatcgaagtg aaagacgatg
      841 ccaaacatgt ggggggctgt actttatttg gtaaaggatt cggtaattac gatcaggata
      901 tcaaattaaa agtttactac agctgatatt tttcttttct attttataaa acaaaaacag
      961 ccaatcccta tgaagggacg gctgtttttg ctttattgtt atttccagaa atcatccaaa
     1021 acggaaattg gcaaatgtcg tttatgttgt gtcttgttcc accatgcttc gattttttct
     1081 tgtgcttgag cgggaacttc ttttccttct agataatcat caatttcatt atatgtgaca
     1141 cctagtgcga cttcatctgc aatcattggt ttcccatctt caagatctgc agtcggaatt
     1201 tttgtgtata atttttccgg tgcattcaat gcttgaagca attgtttccc ttgtcgtttg
     1261 tccaaacgga ataatggcaa aatgtcggct ccaccatcac caaatttcgt gaagaatcca
     1321 gtaatgtttt ctgcagcatg atctgtccct aatacggctc ctgctctttc tccagctacg
     1381 gcatattgcg taatcatacg ttgacgtgct ttgatatttc ctttgttaaa atcgctgatt
     1441 cccacacctg cttcgcttaa tacttgtact tgtgcatcaa cagctggttt gatatttact
     1501 cgcagactaa catcgggctg gataaagtca agagcagctt ttgcatcttc ttcatctgct
     1561 tgttcaccat aaggaagacg aacagcgata aattgatagt tttcatcttt tgtttcttca
     1621 cgcatttctt ccatcgttag ttgcgccaaa cggcctgcta aagtagaatc ttggccgccg
     1681 ctgataccta gtacaaaagt tttaagaaac ggatattttt tcaaatatgc tttcataaag
     1741 tcaatacttt tacgtatttc ttcttttgga tcaattgttg gtttgacacc taattcattg
     1801 ataatttctg tctgcaggct catttattct cctcctatgc ttctgtatga agttctgcta
     1861 cgcttttttt cattcgttcc atgatagcca ttttatggtt ccagcattca gtagacagat
     1921 cgactgggta tttttgcgga ttcaagtctc gtttatactc ttcccatagt gaatctagat
     1981 tttcttttgt atattctttg atttcgttta gtgcaggtaa ttcgtaaact ctttttcctt
     2041 cgataaagat atcttgtaaa actgggcgag ctgtaaagtc tcgaacagtt ttattgataa
     2101 acgtatgcac tggatggaac atgaagatag cttcttcttc tcgagggtct tcatcccata
     2161 atgtcacgta gtctccttca gatttcccat caaaatttcg agtgatgcgc cacacttgtt
     2221 ttttccctgg agtagttact ttttctgcat tgcttgataa cttgatcgta tccatcattt
     2281 ttccttcatc atcttcgatc gatactagct tgaatactgc accaagggct ggctgatcat
     2341 aggctgtgat caattttgtc ccaacacccc atacatcaat tttagctttt tgcatcttta
     2401 aattcaaaat cgttgcttca tccaagtcat tcgaagcata gatttttgct tccgtaaatc
     2461 cggctgcatc taattgttcc cgaacacgct tagatatata ggccatatct ccactgtcga
     2521 tgcgtacgcc ttggaagttg attttgtcac ctaattcttt cgccacgcga atcgcacttg
     2581 gcacacctga tttcaacgta tcataagtgt cgactaagaa cacgcagtct ttatgggttt
     2641 tagcgtaggc cataaatgct tcataatcat ttccataaga ttggactaac gaatgagcat
     2701 gggtcccgct agctggtatc ccaaatatct tcccagcacg tacattgctt gttgcatctg
     2761 ctccgccgat atatgccgct ctagttcccc agactgctgc atccagttct tgtgcccgtc
     2821 tagttccaaa ttccaatagt ggatcttctc cgatgacaga tttgatacga gctgcttttg
     2881 tcgcgatcaa cgtttggaaa ttgaccatat tcaacagtgc tgtttctact aattggcagt
     2941 gcgctaaagg cccttccact tggattaatg gttcatttgc aaaaactagt tctccttcac
     3001 gagcagaacg aactgttgct ttaaattcaa aattttgtaa atactccaaa aaaccttcag
     3061 gatagacttc taatccacgc aagtaagcaa tatcggaatc attgaacgtc agattctcta
     3121 aataattgac taaccgttcc aatcctgcaa aaaccgcgta tccgtggtta aaaggcattt
     3181 ctcgaaagta acattcaaaa acagcatgta aatcagctcg acctaattcc cagtatgtct
     3241 gcatcatatt gatttgatat agatccgtat gcagcgtcaa actgtcgtct gggtaagtat
     3301 tattcatctt gtattactcc tcttggcaag ttttcatttc ctacccatta taacaaaaaa
     3361 aattaatagg aaaagaaatc ttccattttt tctgaaaaag cacatgtttt acggatattt
     3421 cttgc
//
LOCUS       DAIGOK010000181         3323 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.185, whole genome shotgun sequence.
ACCESSION   DAIGOK010000181 DAIGOK010000000
VERSION     DAIGOK010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.185"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(464..826)
                     /locus_tag="NOA01_002559"
     CDS             complement(464..826)
                     /locus_tag="NOA01_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HCI0633707.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            1049..1801
                     /locus_tag="NOA01_002560"
     CDS             1049..1801
                     /locus_tag="NOA01_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCI0633708.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(1941..2864)
                     /locus_tag="NOA01_002561"
     CDS             complement(1941..2864)
                     /locus_tag="NOA01_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCI0633709.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFSFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(2879..3151)
                     /locus_tag="NOA01_002562"
     CDS             complement(2879..3151)
                     /locus_tag="NOA01_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633710.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT         1090 a          623 c          541 g         1069 t
ORIGIN      
        1 cgagaaagaa tacttatttt attcaatgta tccgagacac gaagaaatta gcgttttcta
       61 tttttcttac ttgataaaat aagtagattt tcctactaca atcgtagccg ttaagatacc
      121 gatgattgaa caaatataac caaatgtctc tacaacataa tcaatggcaa aagtttttga
      181 agcaataata cgtctttcct tttttgcttc ttatccccaa agaaacggat ataacttatt
      241 atgtattgaa tttttttgga aaggcgaatt atgacaagat ataccttgcg ttgaaaaaac
      301 tcgcggaaaa aatatcacac gtttttaaaa agatacaaag taagaaagca gctgtcaata
      361 gaatggaaac aaaaatggcg agacatcaat cagtctcgcc ttgtttttta ttgtatacat
      421 tagatatgga gcaggttacc ataaagatga ttattagtcg ttattaaata gaatccttcg
      481 tatttttttg ttctatcact acaatatctt tatttggacg tttgaattgt attccggcac
      541 gttctagctc ctgtttcact cctacatgga ctttgacatc ttctcgtgtc atttttaaaa
      601 cgtgatgaat cgcatagtcg atttcttgcc ttgtgatttt tttcttcgta ttgatcatca
      661 cgacttgctt tttatcatat gtgtctacca atagcagttg tgttcgatct aacgaaaata
      721 atttgtaata attaatagcc gtattcccaa aaaagaattc cactacttta ggataaaatg
      781 tcttaatatt taaattacta ttcacaggtg tttcgattag cttcatctca ttcctctttt
      841 ctatatagtc gatttagatc caatcttaag ttatgttacc atctttccta gcattaagga
      901 atcggttaca attgaacctt taaagacaaa aggatttacc ttttatcact taatagtata
      961 acataatttc gtgagaaaaa aatacaattt tttcttgacc atctcgttac gtaaaggatt
     1021 atgcttacat ttgtcaggag gaaaaataat ggagtacaca ataaagaaaa tgtcagagat
     1081 atcaggagtc agtccgagaa ccttgcgctt ttatgatgag attggactgc taaaaccagc
     1141 taggatcaat tcttcaggtt atcggattta tggcaaaaaa gaagtcgatc gtctgcaaca
     1201 catcttattt tatcgaacaa tggcattcaa gttggatgat atccaagagg tcttagacaa
     1261 tccatctttt gaccaccaga aagctttaat caagcatcag caaatgttgc tggaaaaacg
     1321 ggcacaaatc gataccctct tgacaactgt ccaacagaca cttgatatgt acgaaggagg
     1381 aagaaaaatg agtgacaaag aaaaatttga agggtttaaa caacaaaagt tgaaagaaaa
     1441 tgaagaatcc tatggacaag agattcgaca aaaatacgga aaaaaaaagg ttacagcatc
     1501 caatgaaaaa tggatgaata tggataaaga aacgtatgaa gaaatgcaaa ctgttgaaaa
     1561 gcagatgtta tttgatctca ctctttactt aaaagaacca gaagatgaaa gcttagcaga
     1621 ccgtatcttc caagctcata agaagtggtt ctcttacagc tggcctgaat atcagccaaa
     1681 agcgcataaa ggactaggaa tgatgtacga agccgatgaa cggttcactg catattatga
     1741 cgagcgaagt ggtcatggtg cagcaaaagc tttaaatgaa attatccaaa aaaaggcgta
     1801 atcttttaca tgatccaaca aaaaagtaca cgtaaaatga cctaataaca aaacagcaga
     1861 cacccaagtt agatttttat gctaactttt ggtgtctgct ccattctttc acaagttttt
     1921 tcatcctact ttaaatatat tcatcgttga ataaacgtat aaggtatttt cactttatcc
     1981 tttttttctg taagaaacaa ttgaaatgcc atttctccgc aagcttccag atggtggtcg
     2041 atggttggga aaccaagaag ctcgctgatc agcaaattat cgcgtccaat caccactggt
     2101 gtcttatctt gcagatagct ttgcaagatg cctgctgcaa ccatatccga atttgtaaag
     2161 acaccatcaa ctccaagctc tttaaaagag aagcctgctt gttttccttt ctctgcatct
     2221 atacaatccc aaaaaacaag tgattcatca aaagatggaa aaagttgatg acatagctgc
     2281 aaagtgattt ttgagttata acttagcctg ccacttcgcc ccaacgtgat gcccaattgc
     2341 tttatacctt gctgcttcaa ataagtaaga ccttctgtaa ttgattgttc tcgatcaata
     2401 aagacacagc ttgcctgttt tcccttgatt tcttcacaaa aaactagagg tccatatttt
     2461 tgatatgcca ataaatcatc tagtgtattg gcacgagacg taatgatcaa tccatcgaaa
     2521 gctttagctg caaattcttc aagatacctt cgctcttttt ctttatgata gcccgtcggt
     2581 aaaagtgtca ctttgtaatc ataatcaaac gctgcttcaa tgatcccact gatcaactga
     2641 tcaaaatacg gatgattggt ataaggcaga atcactccaa tgcttttcgt ttttccataa
     2701 cttaactgtc tggcatttgc attagggata taatcgactt ctttcatgac cgcaagtatt
     2761 tctttccttt tttcctcatc tacatatgga tgtccattga ttacacgtga aatagtagaa
     2821 actgaataac ctgtgagctt tgcgatatct cgaatgtttg ccactgctgc ttcgccacct
     2881 atacaaaaag attttcttgt aaatagtcta ccacagctgg accgcattca aaaaactgat
     2941 acatttttcc ttcatgcgaa aaactgatcc gctgcccgcc ccaatatata tcttttgtca
     3001 aattttcgat ttcattcatt ggtatttctt ttactatatt cctattcaac ccttgaaaac
     3061 tgtacaacgt taatacggat cccttgaact cgacaaacgc ataaaaataa ttgttcgttg
     3121 tgccatctgt tatctctaca taagtattca ttgcaagccc tccttttgat actttgagta
     3181 tagtgtatga aacgcgtctc atagcaagga gtttgttttg ccaaagaaaa gttttattcc
     3241 tctttcatta ttaccaaagt ctcttatttt aaattgaata cattataatg atactgtaaa
     3301 tatatcttta ttattagtga atg
//
LOCUS       DAIGOK010000182         3283 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.187, whole genome shotgun sequence.
ACCESSION   DAIGOK010000182 DAIGOK010000000
VERSION     DAIGOK010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.187"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(41..571)
                     /locus_tag="NOA01_002563"
     CDS             complement(41..571)
                     /locus_tag="NOA01_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HCI0633711.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(670..1020)
                     /locus_tag="NOA01_002564"
     CDS             complement(670..1020)
                     /locus_tag="NOA01_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HCI0633712.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(1177..2706)
                     /gene="ahpF"
                     /locus_tag="NOA01_002565"
     CDS             complement(1177..2706)
                     /gene="ahpF"
                     /locus_tag="NOA01_002565"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCI0633713.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     RTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(2716..3279)
                     /gene="ahpC"
                     /locus_tag="NOA01_002566"
     CDS             complement(2716..3279)
                     /gene="ahpC"
                     /locus_tag="NOA01_002566"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HCI0633714.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
BASE COUNT         1029 a          672 c          550 g         1032 t
ORIGIN      
        1 tgttggtttt tatacggtct attttaataa ctaaaaatga ttaacgtata gtcatttttc
       61 ttattctgat gaaattgata atctcctgga tgcccatgaa aatcaaaata aagccgaata
      121 tttggaatag aagcatcaaa cttttgaatg gattgaacaa taagacgaat ccgccaatca
      181 acaacagaat actactataa acactccaac ttttagcttt tgtattcatg ctgaagaaca
      241 actggaacaa gctgttgatg ataacagcta atccaagaag aatcggcagg atagaaacga
      301 ttgcaggtgc aaaagcatag acgatcagag ctaagatcag gaaaaaaaga ccactgaaca
      361 gaccgatact ccaagaacct gttttccttt taaaacggta agcctcaaaa agattcaaca
      421 acccaagcaa aacaagatag gcggtgatca gataaccgat aaaatgaaaa acagcagaag
      481 ggtttagcac aatcgcaatc ccagaaataa tataaacagc agctcttaat aaggcgtatt
      541 tttgaatatt ttgtttaatc gtctcaaaca tataagaaaa cctcctttga cttattttaa
      601 cgtgaactag tcattttacc aaattaaatg ttacaaaatg agcgaaggag aagaatatct
      661 ctggaaaaat caacttctga ggatcttttc catttttttg ccttttgcta gctcatcgat
      721 cattttatct aaataacgaa tttctttcat caagttatcc tcgatgtctt ctactcgtac
      781 accacaaaca acacctgtaa tcaaggaacg tgaaggattg gggacagggg cttccgcaaa
      841 aaattgttcg aaagtcactt gtttttgtag ttgatattct agttctgttt gggaataacc
      901 cgtcagccaa caaatgattt catctacttc ctcttttata cgatcttttc tctccgcttt
      961 tgctacatac ataggataaa cgcttgcaaa acttgtcgta aaaatacgat tttgcgccat
     1021 aatcagtcac ctttcttttt ttcaaattat ttactactta tagtatataa gaaaaagaga
     1081 agaaaaagcg attattcaaa aaaacaaaaa ggattgtgac aaaattcttg tcaccatcct
     1141 tttttccgct ctctgacttt ttacaatctt catagctcaa gtgcgcatca gataatcgaa
     1201 tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca atgatttgtt tgtatggact
     1261 atctgtacaa tcaccagctg cgaaaacgcc ggggatattc gttgcgccat ttttctcggt
     1321 cactatttca tttcttggat tcatttcgat cgttctatct agccaatccg tattagggag
     1381 taagccgact agaatgaata ctcctgaaac agctagtgta tgcatctcca tcgtacggcg
     1441 atcgatatag gagagagatt ctacctgatt cgtaccgctg atttctttcg tcgctgcatg
     1501 tttgataaca gtgacattcg caagagagac tagtttatcc tgtaagattt gatctgcttt
     1561 taattctggt aagaattcta gaacatatac atgttttgct aacccagcca aatcgattgc
     1621 tgcttcaaca ccagaattac caccaccgat aacagcaatc tcttttcctt taaataaagg
     1681 gccgtcacag tgtgggcaat aagcaattcc tttattttta aactcttttt cgcctggtac
     1741 attgatagaa cgccagtgcg cacctgtcgc gatgatggcg gtttttgctt taagtgatat
     1801 accgttagct aagccaatat taataaaatg ttgctcttta tttaaggaaa cggcctgttg
     1861 attcatcata atgtcgatag ggtaactacg cacatgactt tctacttgct gcatcaattt
     1921 tggaccttct gtgtaagagg taccaatcat attttcaatc cctagtgttt caacgacttg
     1981 tccgccgaaa gaatcagcga caagtcctgt acggattcct ttacgtgctg catagatagc
     2041 tgcactacta gctgctggtc caccgccaat cactaataca tcataaggtt ctttttcaaa
     2101 caactcttct tcaatcaaag ggccagtgat tttttcaaca atttgttcga tcgtcatgcg
     2161 tccgctacta aattcttctc catttaaaaa cacagttggc actgccatga tttttttatc
     2221 atcgatttct ttttggaaca tcccaccttc aatcatcgta tgagtaatat ttggatttaa
     2281 tacagctaat atattcaatg cctgtacaac gtcggggcaa ttatgacaag tcaagctaac
     2341 ataactgtca aaatgcaatg tttgtgttat ttttttgatt ttaagactca aagattcact
     2401 gattttcggt gcccgtccac taatttgtaa catagccagt aaaaaagagg aaaattcatg
     2461 accgaggggg ataccagcaa aaacgattcc actttctttg ccaactggat tgatagaaaa
     2521 actaggtgtg cgcatcgaat ctcttttttc ccaagagatt ttaggagaca tttctgctat
     2581 ttccttcgta aattcagcta gtttttgact attttcacta ttatccaaac taacagaaaa
     2641 aacaatagga gattcaatca gtgctaaata ttgttcgaga ttttgtttag tttctttgtc
     2701 taacatatcc accacctaaa tctttccaac aagatcaaaa cttggtttca atgtttcgcc
     2761 actttctttc cattttgccg gacaaacttc acctgggtgg gtacgtacat attgagcagc
     2821 gcgaatctta tcaatcagtg cactagcgtc acgaccgatt ccatctgcat tgatttccat
     2881 agcttgaacg attccgtctg gatcaataat aaatgttcct cgctgagcta atccttgctc
     2941 ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac ggatcaccga tcatcacgta
     3001 ttcgatttta ccgatagcat ctgatgtatc atgccatgct ttgtgagtaa agtgggtatc
     3061 ggtagagcaa gaaaagactt caacacctaa agatttcaat gtactgtatt gttcttgtaa
     3121 gtcttctaat tcagtaggac aaacaaaagt aaaatcagct ggatagaagc aaagaatact
     3181 ccagttcccc ataagatctt tttcactgac ttctaaaaat tctccttgat gataagcatg
     3241 tgcattaaat tcttcgattt ttttcccaat caatgacata gta
//
LOCUS       DAIGOK010000183         3263 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.188, whole genome shotgun sequence.
ACCESSION   DAIGOK010000183 DAIGOK010000000
VERSION     DAIGOK010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.188"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            50..958
                     /locus_tag="NOA01_002567"
     CDS             50..958
                     /locus_tag="NOA01_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCI0633715.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            1099..2712
                     /locus_tag="NOA01_002568"
     CDS             1099..2712
                     /locus_tag="NOA01_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCI0633716.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKKPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         1080 a          573 c          559 g         1051 t
ORIGIN      
        1 agtcattaat tataaataga aagcttttta tataaaaaga gaaaggaaag tggttctttt
       61 gttaacaaac aaccaatcct ccaatcttgt caaaaaaaca ctaacctatt ccatagatac
      121 ccatcttcat ttatctgctg ctccctattt tcgactacta ataaatgaaa gcgctaattt
      181 atatgctgaa tttgcacata aatctataga actagataat ccttcccttt tattcattga
      241 taaacagcaa gaagtccaaa tcttttctac tcaaaaggaa acacaaactt ttatcattat
      301 ccagttttat tttgatgagc aattattagc acatattacg ggacaatctt tttctataga
      361 tcaagaaata acatctatca ttacttttct gaaaaatgaa cagttcaaaa ttgattatct
      421 aaaacatagt aagccagcta tttcctatga tcaggaaatc aattcttttt tcatactatg
      481 ccaacagatg agcattcatt tgctttcctt attagttact tattgtctgc aaaaaattca
      541 attgtcttcc ttaccaaggc attatcaaca ggaacttgct ttattgacaa taccttcaaa
      601 tgagaaagtc attcctaaaa aaaagcatat tctttattca gatgaattag ttgatccaat
      661 catacgatat ttagaagaac atctttcaga aaattttttg cttgaacaat tagcccaaat
      721 tttttttatc agtccagcca gcttaaaaaa acattttaga aaagtgaatg gaaaaagtgt
      781 tattagctat tttaaagaac taaaaatgga aaaagcaaaa cgtctcgtgc tagaaaatga
      841 attaacctat acagaaattg cagatatatt aggatttcat tcgatccatc atttttcttc
      901 agcttttaaa aaatatacag ggttatcccc ttcaaagtat tatctatcct taaaatagaa
      961 aaaaattttt aggttttatc cacaaattat ttctctatat tttctgtcta ttcaaaataa
     1021 acaataattt tccggcacaa ttatttgctg ttttttacca taacatagta cgatcaacgt
     1081 agaaaaggag atgtttttat gaaagttgtt gtcattggcg gtgtagctgg cggtccttca
     1141 tttgccactc gtttccgtcg attgaatgaa gcgcacgaaa tcattatcta tgaacgcgga
     1201 gaaaatattt cttatgcaag ttgtgctttg ccttattatt taggaggtgt gatcactgac
     1261 cgtgactcgc tgatcgaacg tacaccagaa atattgaaaa caaaaaacaa catcgatgtc
     1321 tttactaaac acgaagtaac agcaatcgat ccttctacta agcgattaac agttaaagac
     1381 ctatccacaa atgaagaaat aaaaacagat tacgataagt tgatcatctc ttctggtgct
     1441 agaccagatt atccggatat tcccggagtt tttgaagcag aaaacggctt tgtcctccgt
     1501 agtgtgacgg atgcggatcg aatcaaatcg ttccttgaag aaaaaaaacc acaacatgtc
     1561 gtcattcttg gtgcaggtgt tatgggtctg gaattagctg agaatttcaa gcatcgcggc
     1621 ttagacgtga ctttaatcga tcaattgcca caagtcgctt tcccttatga tccagaaatt
     1681 gctaatttag tttatgacaa attgctgaaa gaaggattag ccgttcattt agaaacaaga
     1741 gttactgaga tccgtgataa aggtcgagaa atagtattat cagatggctc cgtcctttct
     1801 gctgatatgc taatttttgc tgttggtgtt tctccgaaga atgaagtggt gaaagcaacc
     1861 ggcatacaat tatcagatac aggtcagatc attgttgatg accagttaca aaccaatctg
     1921 ccggacatct atgcgattgg cgatattatc gaaacgacta gtgtagtgac tggtcagccg
     1981 atccaaagta tgctttccag tgcggccaat cgtcaaggac atatgctggc agatatttta
     2041 aatggtacgc ctatgcgcta tcgcggatat attggtgcag gtgtcgcaaa aatctttgat
     2101 catacagcaa gttatgcggg aatgacagaa catgaattaa aagcatcagg cataacaaat
     2161 tataaaactg tttttatcac tccctttgac catgcctatt tctatccaga agctacaaga
     2221 ttaaatctaa agctgatttt tgatgcagat agcggtcgta ttttaggtgg acaagcattt
     2281 ggagaaaaag gtgtcgataa acggatggga gaactttctg tagcaatcac cggaaacttg
     2341 acagtctttg atttgcctga tttggagttg ccttactctc caccgtattc tactacccgt
     2401 gacccgttga atatagctgg ttatgtcgca atcaatcaaa tgacgaatat cgtagaaacg
     2461 atcaaagcaa gtgatatacc cgaaaacgat ttgaaagaag cgttcttttt agatatacgt
     2521 gaacctaata aagcaccttc tggcagtatc tcggcaacaa aaaatattcc aatgaatgag
     2581 ttacgagatc gaatcaatga aatcccaaaa gataaaaaag tttacattac tttcagaaga
     2641 ggattgaata cttatacttc tgcccgaatc ttggcaggtt tgggtatcaa agcgattttg
     2701 attgaagaat aaaaaaacga ccgcggtggg tagccgcggt ctaggagtta tttttacttg
     2761 aggagtaaaa atgaaaaagt atatagggtt gttggttggt atgtatatag aatagaagat
     2821 aaatgtgaag aaattgtgca ttatttcttc acatttatta ggttatagtt taagaaagtt
     2881 taaacattgc gcaatttaat tattttagct gtttcttttc ataaaaaaac atgttgtgac
     2941 tctgagacaa atcatagtcc atagtgatgt ctctagagta actacgccta atattacgag
     3001 gcatcatatt aatctatcag cactagtacc taaactagca tccctttcga atattttttc
     3061 ttatataata aagtttgctt tcactcctct aatctcacca aatcgactgg cttgcgttgt
     3121 gtgatactag gaactgattg gtttagaacg acttttctat tgtaatcata tttttcgcca
     3181 tcctcgttgt cttctaatcc ttcaatgaat gatccctaat gtgtgtatta aataatttaa
     3241 tttttttgtt cctatttatt taa
//
LOCUS       DAIGOK010000184         3254 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.189, whole genome shotgun sequence.
ACCESSION   DAIGOK010000184 DAIGOK010000000
VERSION     DAIGOK010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.189"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(471..1085)
                     /locus_tag="NOA01_002569"
     CDS             complement(471..1085)
                     /locus_tag="NOA01_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCI0633717.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(1691..2068)
                     /locus_tag="NOA01_002570"
     CDS             complement(1691..2068)
                     /locus_tag="NOA01_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633718.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(2368..3249)
                     /gene="galU"
                     /locus_tag="NOA01_002571"
     CDS             complement(2368..3249)
                     /gene="galU"
                     /locus_tag="NOA01_002571"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCI0633719.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          989 a          667 c          499 g         1099 t
ORIGIN      
        1 acttataaat atccattatc tatccttcaa tttttgtgta ttttatttct attagaaaaa
       61 ggtatcctgt tggacaattt tgttatatca ggtaaacttc attgcaataa tgataacaaa
      121 tgtactataa ttactttgta aagagttttg gggaagactc tttcatcaac gtgtattaag
      181 agacaagaaa gaatccatat ggattctttc ttgtctcttt ttccactact ttttattttt
      241 ctgacactac atattcttat aactcatttc tcataactgt gaaacaactt ataaaaataa
      301 caaactttaa acaaaacaag cttaaaatca aagatgatcc atcttaaaat aactaaaaca
      361 aaaaaagtag tcgttgttaa tcctcattaa taatcgtttt ctttttttca aacgctccat
      421 ttttatgtgg aacattattt ttttaaggaa ctttgtcaac atgtggataa ctattttcca
      481 cttgtgtcct ggactagctg ttttgctgta aaatacaaaa cttgctgcag caaaaagatc
      541 gttgcaagag aggctgttcc gcagatcgct ggatctagat ttggcaaagt caagactgca
      601 tcatgtaaat tggcgtggtt gtccaaaacc aaatcagcaa attcaaataa tcgcaaacca
      661 ctgctatgtt gcgatttgat acttcgtgac aaatcaaatc cagtaacaat gattaatgga
      721 tgaccatttt ctttgatcca ctgagctagt tcgatgattg ccgggtttct tccttcattc
      781 gaaagaagaa gaaaaatgtc ttctgatttg acagaaagct gatcaataat tgcctgtcct
      841 gcaccttcta gcgtttcata gacacctccg gctggatttt caatcaatat acttggtaca
      901 agatccgggg tttgatccca aaattcaaat gctacaccac ttagtgtgcg gctagcatat
      961 atctgcaatc taccaccttg attaatttgt ttcgcgatcc aagtgccagc ttgaagcatc
     1021 agcggctctt ctatccttaa tttctcttcc aattggtgat aatagtcaaa ataacataaa
     1081 ctcatttatc tctcccgcct agctatactt gacatgaata gaaacagctg cctaatctaa
     1141 ggaagtacat aaaaaaacag tagttagaca gtaacacatg tggtctttgc tgtttcaaac
     1201 acaaaaaagg cattgaacga atacgcccaa taccttgaca tagcaaataa aacaacgtgc
     1261 agaccatgga aaatatctac ataggggtag aattttcacc ctagattagg tacaataaat
     1321 taggtcgatg cactgcattg actatggcaa aatgatcggt cagggtgtat gtgtgtgact
     1381 ggtcattccc tgattcttag cgccaactaa gggtcaggga gccttttttt attcagctgt
     1441 ttctatctga atttattgta agtaatctct tatccaagtg caagcaagaa aatacaaaaa
     1501 aacattggta tgatttatat atatataaat aacataaaaa aaggaatggc ttatcccgat
     1561 acaacaagca ttcctttaaa aaagattctt ttatataagt gaaaaaagct tttttccacc
     1621 aattaaataa atctctcatc tcaatagttt atccattatt ttatcttttt ttgcgtatac
     1681 ctactaaata ttaccgtctt cttctcgttc ttctactttg ttcgagagaa tctgtactag
     1741 aaaaagtagt tgtatgtcta ccttttctgc ctatccgaga ctttcttttc atatatggga
     1801 tataaatgat tagatacagt acagcaaaaa ataaagcaaa tacgcaagct tcccaactac
     1861 ctaacgccaa ttcaaccatc aaggtagcaa ccccaacgga tgctgcatac cagataaaaa
     1921 tcagaaacgt ttcttttttt ctgaccatgc gaaacatgat aaatagacta ataagcaata
     1981 gtggcaacac aaatatccaa ttgcttcctt ccatcagcca cttgagtata tcaagcagcg
     2041 actgtgcaac agcgactaat tttcccatag tgaacacaca ccttttttta taattaaaaa
     2101 attcgccttt ttattctact atgaatcttc tggcttggct ttcatttctc ttatgtaatc
     2161 attttctttt atacaactta aaaaaaaatt aaaccattag cataatattt tctgaatcta
     2221 ttcacgaaag tctttttcta tctttcgttt tccacgactc aactgatcta gagtcaaaaa
     2281 actaaaacga cagttgcgca aatgacgcac aagctgccgc tttaataaca aatagccttt
     2341 cattctcagc aggccactta tttccagtta actttctgtt cttagctttt tactcaactg
     2401 gataatatat gcacttaatg aagtcccaat ttccgaatgt tgaagtccat attcaatatt
     2461 tgtcttcata tatcctaact tatcgcctac gtcgtatctt tttcctttaa actctctcgc
     2521 atagaccgcc tctgtctggt taagagtctg aagtgcatcc gttagctgta tctcgtttcc
     2581 tgctcctggt tcttgagttt ctaatatctc aaatatttta ggagtcagta gataacgtcc
     2641 gataatggct aaattacttg gtgcttttcc catttcaggt ttttctacaa acccttttac
     2701 tctgcaacga cccttttcgc tatctgtttt aggatcaata attccgtatt tagatgtttc
     2761 attttctggt acacgcatga cggcaacagc ggaagcttgt gtttcttcat aatccatcat
     2821 caattgttga gtcaatggga tatcatcttt catcaaatca tcccccagca tgacgacaaa
     2881 cggttcattt cctataaatg cttttgcttg aagcacggca tcccccagtc ctcttggatg
     2941 agattgacgg atgaagtgaa gattgacttg agtcgtttct tcaactaaag cgagtaattc
     3001 tgttttttcc ttttcccgca aattgttttc caattcgata tttgcatcaa agtgatcttc
     3061 aatgggtctt ttttcttttc ccgttacgat caagatgtct tcgattcccg aatccaatgc
     3121 ttcttccaca ataaactgga tcgttggttt atccacgatt ggaagcattt cttttgccat
     3181 ggcttttgtt gccggcaaaa aacgtgttcc tagcccagcg gcaggaatga ctgctttttt
     3241 gaccttcact tgct
//
LOCUS       DAIGOK010000185         3182 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.190, whole genome shotgun sequence.
ACCESSION   DAIGOK010000185 DAIGOK010000000
VERSION     DAIGOK010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3182
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.190"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            108..1847
                     /gene="yfcC"
                     /locus_tag="NOA01_002572"
     CDS             108..1847
                     /gene="yfcC"
                     /locus_tag="NOA01_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCI0633720.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            2004..>3182
                     /gene="iadA"
                     /locus_tag="NOA01_002573"
     CDS             2004..>3182
                     /gene="iadA"
                     /locus_tag="NOA01_002573"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCI0633721.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     S"
BASE COUNT          971 a          537 c          748 g          926 t
ORIGIN      
        1 tttctaataa attattcatg tggaaatgtc atttttactg tataattcat ttgattatcc
       61 attttttatt catcaaaatt actgaaaaaa gaaaggaaga aataaatttg aagaaaacaa
      121 attcttcagg aaaaccgaaa cgcgacttca ctaaattatc gacaccccat acgtatgtca
      181 ttatttttgg tgtagtcatc tttgcatgga tactgacttt tgtcgttcca gcaggaaagt
      241 tcagcacgca agatattgaa tacaaagatg caaatggcga aacaagtact cgtactgttt
      301 tacgtcaaga ttccttccgt tatgcttacg agctggataa gtcgtatgtg tttgatcaat
      361 tagaagaact gcaagatcat ccagcagaac gtgaaaaact ggatgttccg gaaaaaggat
      421 tggaaaaagt cattgctgac ggagaaaaaa atcttacgca ggaaaaacta gatgaaatct
      481 ccttgacaga tgatgtgctt tacgatcagt acggcgaaaa tatctatgat acttctaaaa
      541 aattgcataa aacagccaaa atatggggaa cagatgattt tggcggcttt ggtttcttaa
      601 attttgtttt tgaaggacta gtatctggag ataagtatgg atctgctgta gggatcgctg
      661 cattgatcct tgttgttgga ggtgcttttg gtattattat gcgtaccgga gcgatcgacg
      721 caggtatcta tgcgtttatc agtaaaacta agggactgga gcgtttagct ttaccgctat
      781 tattctttgc tttttcattc ggtggcgcta cattcgggat ggcagaagag gtaattccat
      841 tctcaatggt gatggttcca tttgtcattg cgttaggata tgactcgatc gtagcagtaa
      901 ccgtgactta tgtcgcatct caagtcggaa atgcgacctc ttggatgagc ccgtttagcg
      961 tagcggtggc tcaagggatt gctggaatcc ccgtgttgtc tggtgcgaca ttccgattga
     1021 ttatgtgggt agttgttact gcattagccg ctggttatat gatgatatat gcagaaaaaa
     1081 tccgtaaaaa accagaaagt tcgttgactt acaaatcaga tgattatttc cgttctcata
     1141 tcaaaaagac atctgatgaa aataaaaaat tcatgcttgg gcataaattg atcttattag
     1201 agatgttagt tgtccttgtc tggatcgttt ggggcgtgac tcaaaaaggc tattacattc
     1261 cagaaatcgc ttctcaattc tttgtgatgg gattagttgc tggaatcatt gctgttcttt
     1321 tcaaattaga tggaatgaaa gtcaatgaca tcgcttcttc tttccaatct ggtgcagctg
     1381 atttagctgg tacagcaatc gtagtaggta tggccaaagg gattcttctt gtcttgggag
     1441 gatctgatgc atctgttcct tcagctttga atacgatttt gcatgggatc gggacggcgt
     1501 tgaccggtgt tccagctgtc attggtgctt gggcgatgta catcttccaa agtttgttca
     1561 acttggttgt tacatcaaat tcaggacaag cagctttgac tatgccaatc atggctccac
     1621 tggcagattt agtaggtgta tccagacaag tagcagttct tgcttatcag cttggttcag
     1681 gttttatgga cgcttttaca cccgtatcag ccagcttgat tggtgtgcta ggtgttgcac
     1741 ggatcgaatg ggcaaaatgg gcaagattcc aaatcaagat gcaaggattc ctgtttgttt
     1801 taggaactat tttcatcatg attgccattg caataggtct gcagtagtac tggagacctg
     1861 taaataaaaa agtaatccaa acagtagata aggactccaa gaaccgtcat tttacggtaa
     1921 aatcaaagga gtaaagatcc tatcgtttgg attatttttt aagatcctat catcagttat
     1981 acttaaagaa agaggaaaaa tgtatgaaaa tcattcgaca aattgaagtg tttgcaccag
     2041 attacttagg aaaaatggat gtactgattg ccggtgggaa gattttggct gtagaagaac
     2101 agctagaagg cggctacgaa ggagtggaag tagaagaact ttcaggtgag ggaaaagtac
     2161 taaccccggg tttcattgac tgtcatttcc atattttagg tggaggagga gaaggcggtt
     2221 atcaaaatcg aacgccagaa gtcacattga gccaattgac tacagctggt gtaacgacag
     2281 ttgtcggttg tttgggaaca gacggagaag gtagagatat gactgcgttg atcagcaaag
     2341 cgaaaggtct ggaagcagaa ggaatctcca cttatgtata cgaaggttcg taccgattgc
     2401 cggtaaaaac agtaacagat tctatcatca aagacttttt gacaattgat aaaattatcg
     2461 gaatcggaga aatcgcagta tccgatcacc gttcttctca gccaagtttt gaagagttcg
     2521 ctcgtgcagt agcagatgct cgtgtaggag gcatgttatc cggaaaagct gggatcatca
     2581 atgtccactt aggcggagga aaaagaaagc tagagctatt gacacgaatc gtagaagaaa
     2641 cagaaattcc gatcacacaa tttttgccta ctcatgcaaa tcgcacacca gaattatttg
     2701 aagcttgcgt ggcatttgcc aaacgtggtg gcacgattga cttcactgct agcgaagatc
     2761 ctgatttttg ggagaagaca gatggtgaag ttcgcttcag caaagcactg aaacgattga
     2821 tcgaggaaga tgtttcttta gataatttca cgatgacctc agatggacaa ggaagcttgc
     2881 cttattttga tgaaaacaat catttcctag ggttaggagt aggaagtgcc aaggcattgc
     2941 tagtagggat caaagaagct gtgcaaaaag aaagtatccc tttggaaatc gccttacgag
     3001 ctataaccag taatccagca cgtatattga aattggataa aaaaggaaaa atcgaaattg
     3061 gtgctgatgc agatctgtgt atcttggata aagaaacgct tgatattgat acagttatcg
     3121 ctaaaggaga aataatggtc caagaaaaag aagtcaaagt atgggggact ttcgaaaaaa
     3181 gt
//
LOCUS       DAIGOK010000186         3157 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.191, whole genome shotgun sequence.
ACCESSION   DAIGOK010000186 DAIGOK010000000
VERSION     DAIGOK010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.191"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(61..2424)
                     /locus_tag="NOA01_002574"
     CDS             complement(61..2424)
                     /locus_tag="NOA01_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HCI0633722.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
     gene            2872..3093
                     /locus_tag="NOA01_002575"
     CDS             2872..3093
                     /locus_tag="NOA01_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCI0633723.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
BASE COUNT          857 a          654 c          571 g         1075 t
ORIGIN      
        1 cttaatttga caaataatac aatgtaaaaa attctgtttt gcgggatcga ttttatcatt
       61 ttattggtca agatttttcc atttccaatc caatacttct ggcagatcaa taccattttc
      121 acgaatatag ttttgatgtt cttgtaattt ctgatccatt ttttccgcaa aatcttctgc
      181 ttgacttcct ttgattttta aagctgcgtc ttttgccaaa tgataacgat caagttcatt
      241 tactacacgc atgtcaaatg gtgtcgtgat atctccattt tctttgtagc catgaatcat
      301 taaatgatgg ttatgacgat caaagaaaat cgtgcggacc aatgtttcat aaccgtggaa
      361 agcaaataag atcggtttgt cttttgtaaa atattgatca aattcttcat cgctcaaccc
      421 acgcggatct tgacttggat gacgtaattt caataaatct acgacattga tgaaccgaat
      481 ttttagtttt gggaattctt ccagcagcaa agttacagct gctaatgctt ctaagttaga
      541 ttccgttcct gcagcagcaa tgaccaattc aggttcctcc tcttttgctg tcgaagccca
      601 atcaatgatt ttcaagcctt cattcactaa gacggctgct tcttctgctg agtaaaattg
      661 agcacgcgga tgtttggaag agatgatcaa attgattttt tctgacgagc ggaatgcttt
      721 atccatgact gcgagcaatg tatttgcatc cgcaggcaga tattcacgga tgaactccgc
      781 cttcttttct gccaaatgtg tcaatattcc tggatcttgg tggctatatc cattgtgatc
      841 ttgctggaag actgttgagc ttgcaatgag attcagcgaa ggataatctt ttcgccaaga
      901 aaggtcatgt gatttccgta tccatttgaa gtgctgagtc aacatagagt caaccactcg
      961 caagaatgat tcgtaacttg caaaaaaacc atgccgtcca gtcagaacgt acccttctaa
     1021 gaacccttca tcttggtgtt ccgataattg actgtcgatg acgcgaccag atggcgaaag
     1081 ccattcatca ttttctggtt cgattttttc catccattgg cgatttgtcg ttttgaaaac
     1141 ttgattcaat cggtttgatt ttgtttcatc tggtccaaat atacggaaat tttctggatt
     1201 tttctcaatg atctcgcgaa cgaatttacc aagttcggtc atatcttctg ctttaacgga
     1261 gcctggtttt tcaaattgca aagtatattt tttccaatct ggcataataa gcggtcttgg
     1321 atcgattcca ccattggtga taggattttt tgccattctt tggtcaccag taggcgcaat
     1381 ttctctgatt tcttctaaaa tacgtccttg tgcatcaaat aatttttctg gttcatagga
     1441 tttcagccaa cggagtaaag catctgcatg atccatatgt tcttgatcta caggaatcgg
     1501 tacttggtgt gctcggaagg ttccttcaat tggttccccg tcccattctt ttggaccggt
     1561 ccatcctttt ggtgtgcgta cgatcaatac tggccatttt ggcatcacga catctgttgc
     1621 tgtcttcagg cgagcttctt tttggatgga tttgattttc tcgattgctt gatccatttt
     1681 ttcagccata acaggattta atttttctgg atcatttcct tcaataaaga aaggctccca
     1741 gccaagtccg ttaaagtaat ttgctaattc tccatctgat ttacgtgcca agatcgttgg
     1801 gttggctatt tttgcaccat ttaaatgaag gatcggtaaa acagctccat ctatcactgg
     1861 attgataaag acatttgaga accagcttcc agctaaagga ccggtttctg cttcaccatc
     1921 accaatcacg acagcagaga ttacttcagg attgtctaat acagctccga ctgcatgtga
     1981 aagagagtat ccaagctctc cgccttcatg gatagaacct ggtgtttctg gtgctgcatg
     2041 agaagcaatg cctccaggaa atgaaaatcg cttgaataat ttttgcattc ctgtctcatc
     2101 ttccgttact tctggataga tctctgtgta acttccatct aaataagcat tcgataccat
     2161 gacttgtccg ccgtgtcctg gcccttcgat ataaaacatg ttcaaatcat atttattgat
     2221 gactcggttc aaatggacat aaataaaatt ttggccagga atcgttcccc aatgaccgat
     2281 tggatgtttt ttgacatctt ctggtttcag tgtacgtctc agtaaggggt tatctttaag
     2341 ataaagttgc ccaacagaaa gataattagc agcacgccac caagccgtca ttttatcaaa
     2401 gtattcttta ctagaataat ccatacataa cactcctttt cattattact cttattctag
     2461 cgcagatact cccaattgaa acctaataag cacttctgtt catttgtttg gattttatat
     2521 ctagccaaat gaacaggtat aaaaaaccac gaaatttttc ctttcgctga cgttttttac
     2581 attgatccaa aaaacttgtc gttatcacaa tggctgttcc gcagttcatt tcgctcctca
     2641 tcatgcataa tttgctgaat gacgctaggc cagttaacac attgttggaa aactggggct
     2701 ggatcgagca attcctttcc tgattgatcc gttttatgta aaagtctctg ttatctttgt
     2761 caatatgtga attggtatcc cagctactat gctaatttct acagggatta tccaaaattt
     2821 ggatcgtgac caaatcgaag cggcgtaatc ggaaatatca ataacttcaa cgtgattttt
     2881 cttttaactg tcaacggacc gacaaactca aacttttatg gtgcaggttc cactgatctt
     2941 ctagttactt ggctatacct gactgtcact aaaatagatt acaatctagc ttccgttatc
     3001 ggtatcttga tctttatcta tatgcaaaaa tttatatact atagaaaaaa gaaagtcgaa
     3061 ttttttactt ttctttttct cttttttggt tagctgacac gctaccagac taaagtcatg
     3121 gaactcttag gtaatacgtc ttaatgaaaa caaaaaa
//
LOCUS       DAIGOK010000187         3112 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.192, whole genome shotgun sequence.
ACCESSION   DAIGOK010000187 DAIGOK010000000
VERSION     DAIGOK010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.192"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(254..622)
                     /locus_tag="NOA01_002576"
     CDS             complement(254..622)
                     /locus_tag="NOA01_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HCI0633724.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            772..1254
                     /locus_tag="NOA01_002577"
     CDS             772..1254
                     /locus_tag="NOA01_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HCI0633725.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            1291..2301
                     /locus_tag="NOA01_002578"
     CDS             1291..2301
                     /locus_tag="NOA01_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HCI0633726.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(2416..3102)
                     /locus_tag="NOA01_002579"
     CDS             complement(2416..3102)
                     /locus_tag="NOA01_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633727.1"
                     /translation="MVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAEKSSTQKSAS
                     TETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLAGYSDLQIEY
                     ARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVMLTGKYSYQGV
                     LVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAIPTGNPNDVK
                     QLINVIVMDN"
BASE COUNT         1049 a          500 c          615 g          948 t
ORIGIN      
        1 tattttctcg tattttttcc ttattcaaaa gttttgcagg aaaattgctg aaaaaataat
       61 tttactaagt ggaaaattca aagccattca atcagtgatt tttttcagca caagaaagac
      121 tccttataaa aatagcagat actttgtcaa ttaagtgttt aaccagggat gataaaaagg
      181 ataggattga acaaaacctg tataagctga tgaatagttt cctcgccagt aaaaggttta
      241 ttcgtataaa gctttatcgc caaaatttat catgaccttt tttatcttca atattaaatg
      301 aaatcaattg ttcaatcaat tcccagttga ttacactttt ccaaggaact ttgatgacgt
      361 tatccgtata tacgtagcct gcttcttgga tatttttttc aagggcacga atcgctgctt
      421 tttccggtgc gatagaaaaa tgatttttag caaaactaaa tgcaataata aaggtaccat
      481 ggtcagtaaa cataggttgg ttccatttta cagttgtgtc tagctgggga tacgtttctg
      541 ttatcttagc aaaaaggtct ttgatctttt gttgatgttc tttttctttg atctgattga
      601 tgatcggctg aaatttttcc attctaaaac gcctccatta agtggtttga tttattttac
      661 cagaaaaaca atagttatgt ataaaaaacg aacaaaacac aagtaatgtt gtaaccgcta
      721 tatttatatg ataagatgtc tcaaataata aaatagaaaa gagacataaa aatgattgac
      781 ttggcaaaga aattcttaca cgaaaatata tattttgagt tgtatcatca tcgagccatt
      841 tatacaaatg aagatgcttt ggtaataaag aaagaacaag gatttacagg tacagagacc
      901 aaaagcttat tcttaaaaga taaaaaagat aataactacg tatatctgac ttttactacg
      961 aaacaaacgg atttcaaagc attagggaag atcgtcggta aaaggctttc tattgtttca
     1021 tcagaacgca tggaagaaca aacgggacaa aagcagggcg ctgtttctcc gtttggttat
     1081 gaaaaacaga ttccgattat tattgatgaa gagctcttat caaatgaaaa acttgttttt
     1141 gcaccaggta gaccagatca aacgatggtc atccaaacgg ttgacttaaa caaaatcatc
     1201 cagctacttg agatgccgat atttttgatg ccggcaaacg aaaaaaacaa ttaaccaagt
     1261 aaaatagtgc atacttggag gtgaagcaac atgataagtt atccattatt agaaagaaat
     1321 gcaacgattg gggttactgc accatcctca ggcgtatcag aggaacacca ttcgttactc
     1381 gaatcagctt taaagagaat ggaagaaaaa tctttccaag tagttactgg aaagacgctt
     1441 tggacgcaag ataaggaaaa atcaggtcca gcaagaaaac gagcagatga attcaatcaa
     1501 atgatgcaag atgaaaatat ccaaatgatc tttccgcctt ggggaggaga attattgatt
     1561 gagattctcg attttgtcga ttttgatgct ctacaaccaa aatggatagt aggttattca
     1621 gatattagta cgttgctact tgctgtcaca ttaaaaacag ggatagccac tgcacatgga
     1681 accaacctta ttgatttaag aggagagtac ttggatgaaa cgacagcaat gtgggaaagt
     1741 gtactgaaaa caagagaagg agaatcgatt ctacagcaac cttctgaaaa gtatcaaaaa
     1801 gagtggcccc atgagcattc atcatcttct gttttccatc tcactgaacc aacaaattgg
     1861 caatcagtca gcggagatgc agtaaggatg aagggacgat tattaggtgg ctgtattgat
     1921 acgatcagtc atttaatcgg taccccttat ggagatgtcc aatcttttaa aagaaagtat
     1981 aatgatggaa agccgatctt gtggtttcta gaaaattgtg agctatcgtc agttgattta
     2041 agaagatcac ttgttcagat gaaactggca ggatggtttg atggcagctc ggggatcatg
     2101 ttcggtagaa gtgcagcaga ggatttgtta gattataaaa tgaaggatgt atatgaagat
     2161 ctgtcaaagg aattaaaact accgatcttg tatgatattg attgtggtca tgtaccgcca
     2221 caactgacat tgatcaatgg tgcctatgca gaagtagcgg tagaagatag aaaagcgact
     2281 gttttgcaaa cgtttattta aaaaattttt tcctgccttg gagtttatca aagaccaaca
     2341 aatctaaata gaaaagaaaa tgaaagaagc tgacagtatt ttgccagctt ctttttgtat
     2401 gatctattag aaattttaat tatccataac tatcacattg atcagctgtt tcacatcatt
     2461 tggattacct gtaggaatag ctactacaga tgcattatca ataatttctt gagttgtttc
     2521 tctaatgaac gctggataag atgtttgatc tgcaggcatt tgccaatgtg aaggaactgg
     2581 ataactagtg attgtcccgt catggttact tgaatacact aaaactccct ggtaactgta
     2641 tttcccagtg agcataacca catctttcgg atagactgca ctccctgcat cataaggggc
     2701 gataggtgtt cctgcaggat aatgaataac atgtagttcg aaatcacccg atgtatctct
     2761 aaaagtatta cctctaattg ctagccaaac acgtgcgtac tcaatttgta gatcagaata
     2821 tcctgctaac ggatctattg actgagaaga ctgtgaggaa gttgtgtcta ttgttgaaga
     2881 agaaatattt gtctcaactg tactactaga tgtagaagaa gtattgggct ctgagacttt
     2941 actgcttgtt gaagatgtat tcgtttcagt agaagctgat ttctgagtac ttgatttttc
     3001 agctgaactg gtttgtttga ttgaactttt gtctgtggtc gcagatggat cactattttc
     3061 tttactagga gacgtacatc ctacaaggaa taaagagacc aacaataaaa ta
//
LOCUS       DAIGOK010000188         3046 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.193, whole genome shotgun sequence.
ACCESSION   DAIGOK010000188 DAIGOK010000000
VERSION     DAIGOK010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.193"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(308..670)
                     /locus_tag="NOA01_002580"
     CDS             complement(308..670)
                     /locus_tag="NOA01_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633728.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(744..1124)
                     /locus_tag="NOA01_002581"
     CDS             complement(744..1124)
                     /locus_tag="NOA01_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HCI0633729.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1188..2381
                     /locus_tag="NOA01_002582"
     CDS             1188..2381
                     /locus_tag="NOA01_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HCI0633730.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKCDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2458..3042)
                     /locus_tag="NOA01_002583"
     CDS             complement(2458..3042)
                     /locus_tag="NOA01_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCI0633731.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          951 a          581 c          500 g         1014 t
ORIGIN      
        1 ttagtaacat gtcaataact attttttaaa ttttttagaa atgtgatata acatccactt
       61 cagaatcaac gctgttaaat ttatcatgta tttcttcctt ggtcaactat aaattaagca
      121 ttactttgtg aaatgatttt tcgatcaatt attattattc gttatacaac cgttttcaac
      181 atattttgaa aataaaatgc actaaaaaac tatctttagc agaacatttc attgtatttt
      241 ttggaccaaa caataaaaaa agaagtttga aaaaaattca aacttctttg ttcgcttaat
      301 ctgtatttta gaagaaaggc actccctcta cttcgtaagt tccaagaaca gagcgcattt
      361 gacctggttc ttcccaaata tatccttcgc ctaattcttc tctttgattc ttttgttcag
      421 tttcaaagac aaacaacgga acattttcct ctttaatatg tgtatttgtc aattccatca
      481 aactaatctt gctaacatcg atatgaacta tttcttttaa gaaattaagt atattcgcta
      541 aaccagtctt tccttcagaa agttccgcta acgcaaattc caacttgtca tcaattgaat
      601 ggaccaaaaa ttttttagta ccatcctcca aatgcaacat aattgtacca gcaactcttt
      661 tgcttgccaa aatttccacc tcgatcaact tgttctatgg cttacataaa aattctagca
      721 cattttcaga aaaaatacac tttttatttc ttattttctt tccgttctag ttcagataat
      781 gcttcttcta tccaaatagg taactgctcc gcaatagtgg taaatccgat cttacgattt
      841 ttattcgtga aatacctttt tctgcgatat ggaacagctg gatttgtttt ttctaacgtt
      901 cttaaagata aactgatttt tttggaatat tcatcaatgt cgatgatctg aactgttact
      961 ggctgtccga cttttaacaa tgaatggata tttttcgtat aaccggattg tacttctgaa
     1021 acatgaataa gtccttgcgt ctcgttatca agagaaacga atgcgccata aggctgtatc
     1081 cctgtcactt ttccttcaat tatttgtcca attttataag tcatctactt ccctctcttc
     1141 gcatttatca ttatactgta aatagctcaa aaaaagaagc gacgaaaatg actggaggtc
     1201 ttaagatgat cgaatttgat aaacagattt caagaaaaga tacagaaagt gtaaaatgtg
     1261 acgcaattgc tgagacatat caaatggatg acttacttcc tttgtgggta gcagatatgg
     1321 acttcttgtc tcccggtggg gtagctaaag cgtttagtga atacatcaaa catggaatct
     1381 ttggttatgc cactctgtct gataaactat atcaggctat cattcattgg gaacgccaac
     1441 accatgctgt ggagttgacg aaagaaaata tcgtttttac aagtggtgtg ttaaccagtt
     1501 tagctgcagc agtacaaact tttacaaaac caggcgattc tattttgatt catgacccag
     1561 tttatcctcc attttcctct attatagaaa caaatcaacg tcacatcgtt cgaagctctt
     1621 tgctagaaaa aaataatcat ttcatcatgg acttgtctga catggagaaa aaaatcgaag
     1681 aaaacaatgt aaaagcaatg attttatgca atcctcacaa tcctggaggg cgtgtttgga
     1741 gcaaagaaga attgaaacag ttaagcgaat tgtgtttaaa acatcaagtg attctgttta
     1801 gtgacgagat ccatcaagat ttaactttat tcgatcatac atttacttcc atgctaacga
     1861 ttgatcctaa tttagatgat ctactagttg cttttacttc cgcaaccaag actttcaatc
     1921 tagctgcaat caaaaattca atggtattta tcaaaaatcc agagatgaaa gctgattttg
     1981 aaaaacattt agtgatgaac caacagcacg aaatcaacac ttttggactg attggaaccc
     2041 aagcggctta tgagacgggt ggagaatggt taagccaact gattccttat ctagaaaaaa
     2101 atgttcaaac agttaatcag ttttttgaac agtatcttcc gaaagtgaga ataatgaacc
     2161 ctgaaggaac ttacttaatg tggttagatt ttaccgccta tatttcagac gaccaagaat
     2221 tggagaacac tcttgttcgg aaaggaaaag tcgtattaaa tcctggcatc acgtttggtc
     2281 cttccggtca tgggcatatg cgcttgaata ttgcttgtcc agaagaaact ttactggaag
     2341 gacttaatcg aatcaagtat gctttagaga caatcgatta aatcatttat tcaaaataaa
     2401 aggacgagac cttactaggt ttcgtccttt ttcataatta ctccgtacca taccaaacta
     2461 ttctgaaatt tcaattgttt caatcactac atcatgtaca ggtcggtctt gtggaccacg
     2521 ttgtacattt gcgatttcat ctactgtgtc catcccttct agtacatgtc caaagactgt
     2581 atgtcggaaa tctagccaag gagttccgcc acctttatac gcttcaatga tttctgatgg
     2641 gaatccagca ccttctagtt gtccaagcat attagctggc acatttgtgt tatttacaat
     2701 aaagaattgg cttccgtttg tgtttggacc tgaatttgcc atagataaag ctccgcgaag
     2761 gttgaacagt tcacgactga attcatcttc aaaactttcg ccataaatac tttcgccacc
     2821 cattcctgtt ccagttggat cgccgccttg gatcatgaaa tcaggaataa cgcggtgaaa
     2881 aatcacgcca tcataataac cttttttgga aagttcaatg aaattttgaa ccgtcttcgg
     2941 tgctagttca gggaataatt gaacagtaat gtcaccacgg tttgttttga taacagcttt
     3001 tggtcctttt tcacttttta aatctagttg tgggaatgcc atattg
//
LOCUS       DAIGOK010000189         3001 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.194, whole genome shotgun sequence.
ACCESSION   DAIGOK010000189 DAIGOK010000000
VERSION     DAIGOK010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.194"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            236..1627
                     /locus_tag="NOA01_002584"
     CDS             236..1627
                     /locus_tag="NOA01_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCI0633732.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAESAFPKEKTYR"
     gene            1665..2495
                     /locus_tag="NOA01_002585"
     CDS             1665..2495
                     /locus_tag="NOA01_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCI0633733.1"
                     /translation="MENKNISILLSLYDKEKPEYLKEALASIFEQTVMPAEIVLVYDG
                     PINEKLQAVVTYFQQKVPHLFTVVKLEKNQGLGIALATGLEHTKNRLVARMDTDDIME
                     PHRLEKQLEIFQEYPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFN
                     HMTVMFDKNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYAR
                     RGGSKYLLPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN
                     "
     gene            2467..>3001
                     /locus_tag="NOA01_002586"
     CDS             2467..>3001
                     /locus_tag="NOA01_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCI0633734.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLRRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNYGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNHPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIV"
BASE COUNT         1045 a          427 c          589 g          940 t
ORIGIN      
        1 tatgaatatt gtaacttgtg aataaggaat tagcaggaga atactagttc agcataaagg
       61 aagtgatggg acttctgaga caactttctg ttatgcagaa atacacatat aaacagacaa
      121 taaacgcttt tccccgtttc ttttaatttc ctttacaatt tggttattca ctaggttttt
      181 aaactttgct atggtaagat gatagaggca ttattttatg gttaggagtg tatatatgaa
      241 taaaaatggg gagtggaatg cggcacgcag aatattcatt attcttatgg atgtacttgt
      301 attaaatgta tcgatctatc ttagtttttt gctgaaattc cagggagaag taccagtgag
      361 aaacttagaa acctttcagc attcagcaat ttttatttcc atcatcttta ttgcgttgaa
      421 tattttgtta ggagcttatg tattctataa ccgaatgatc agtgatattg tcttcattac
      481 tgttattgga caagtactaa tagcattggg gatcatggta gttacttttg ctggccgatg
      541 gtttgcattt ccgcgtggcg tcattttgat ctcgtttatc ctaggtacga tattacttag
      601 tgtttaccgg atagtcgttt acaaactgta cttaagagtc agtcgtgata agaaagtcct
      661 tatcgtgggt ttggagaaag atgtcgcacc agctatccaa aattttcaat ctaagaaaaa
      721 caataaacat aaggttgaag cagtggcaat cgataattat tacgaaaata caaaaaaatt
      781 gatcgatgat atggatatcg tctatttggc ttctaaaatc ccagaagatg aaaaaatcaa
      841 catttatcaa ttagtgacta aaaaagagaa gaaattattt ttaaatacga cttttgagaa
      901 tctgacaatg ataaatccga atattatgaa ttttgaagat gaaagcatca ttgaagcttc
      961 tgggttcaga atccaaccag aatacgaact attcaagcgt ctctttgata tcatcgtttc
     1021 gcttatttta ctgatcctcg cctcaccttt tatgctaatc acagcgattt tggtaaaaac
     1081 aacttcacct ggaccaatca tctataaaca agtacgtatt acgaaaaatc aaaaggaatt
     1141 ttcaatctat aaattccgtt ccatgagtgc gacagctgaa gcaaaatcag gaccagtctt
     1201 ggctaaaagt aacgatgcac gtgtgacacc agtaggtaaa tttatccgtg ctgtacgttt
     1261 tgacgaatta cctcaaatct ttaatgtatt gaaaggcgat atgtcgattg tcggtccaag
     1321 gccagaaaga ccatttttcg ttgatcagtt caatgaagaa aatccttatt attacttaag
     1381 acacaatgta cgtgcgggaa ttaccggata tgcacaagta tacggaaaat atgtatcaga
     1441 ttataacagt aaactacgtt ttgatttgtt gtatatcaaa aagtattcct tactattaga
     1501 tttgaagatt ttacttcaaa caatcaaaat cttatttgat aagatgtcct cacaaggact
     1561 agatgaagat gaaaatatca gttcattagc agaaagtgca tttccaaaag aaaaaacgta
     1621 tcgttagatt ggaaaaattt tgagcaaaga agtaggagag aacattggaa aataaaaata
     1681 taagtatact gctttctttg tatgataaag aaaagccgga atatttaaag gaagcattag
     1741 ctagtatctt tgagcaaacg gtgatgccgg cagaaatcgt attggtttat gatggcccta
     1801 taaatgagaa gcttcaggca gttgtcactt atttccagca aaaagttcct cacttgttta
     1861 cagttgtaaa attagaaaaa aatcaaggat tggggattgc tttagcaact ggattagaac
     1921 atactaaaaa tcgcttagtg gccagaatgg atacagatga catcatggaa ccacacagat
     1981 tggaaaaaca attagagatc ttccaagaat atccagaaat agcgattgtt gggagtaata
     2041 ttgaagaatt tactggcaca cttagcaatg ttatcggaag aaggattgta cctgagtata
     2101 atgatgaaat ccgtgtgttt tcgaaaagaa gaaacccttt taatcatatg acagtcatgt
     2161 ttgataaaaa tgctatttta gaagtgggaa actatcgccc attaattgga tttgaagatt
     2221 attatttgtg ggtaagacta ttgaaggcgg gatataaagg atataatatc caagagattc
     2281 tagtacatgc tagagcaggt gctgacatgt atgctagacg tggaggaagt aaatatcttt
     2341 taccaggaat taaaggccga tatcgcgtat ggaaagatgg tctaggatca ctaaaagatt
     2401 ttctgatagt tatttgcgga catcttgtaa ttagtttatt accgaataaa attaggggaa
     2461 aagtatatga atcaaaactt agaaaaacaa actaaacttt ctataatcat acctttttac
     2521 aatgcaaaga atactttaag aagagctgta gacagcgtct taacgcaagg ttatcccaat
     2581 attgaaatct tgttaattga tgatggatct acagatggta gtagtgacat tggtgacgaa
     2641 attgctaatc tagagagtaa agtagcatgt tatcacttgg aaaacgctgg tccatcagtt
     2701 gctaggaact atgggataaa agaagcaact ggaaatttgg taggattttt agatagtgac
     2761 gattattttt tacctgattg ttttaataat gttttgaaag actaccatag gaatcacccc
     2821 gatatcattg cttttggatt aagtaaagga aaaaatatag aaaatgcaaa tttatttgtt
     2881 cccgaaaaga agattggttg ttcaagtgaa gaagcaattg agcaaatgtt tagaagtaag
     2941 gcagtggact tttacgtatg gaataaattt tttaaaaggg aattattcaa tcatatagtt
     3001 t
//
LOCUS       DAIGOK010000190         2921 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.195, whole genome shotgun sequence.
ACCESSION   DAIGOK010000190 DAIGOK010000000
VERSION     DAIGOK010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.195"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            343..1272
                     /locus_tag="NOA01_002587"
     CDS             343..1272
                     /locus_tag="NOA01_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633735.1"
                     /translation="MRSDSRFFVSNLQDDELIRQIDSLLETITESDKRIFLNYVELTR
                     HIIELDKLFNVFRYNLTNLLKHFTIFTNDLIESTGEKLTEDQYYYQINALTINLISSA
                     KTLTESIEVCMKNFLAEKDFKSFKNKILSKPYDEHFSYRFLLHVRNYSQHGHLPVNIE
                     QQRVYFDLDEILTMPHFDLNEKLKSEIDEIKEDISARFEDFPRISYVYTIAKFNLITT
                     EIYLNYLKEVKPILMEMDKEKNELLLNTKFKLTNSDGKSSDVVFYDFDGENYHCFNRT
                     DNSLSMYASIKKEVKKILREEEQYYKEIKNKNQ"
     gene            1441..2871
                     /locus_tag="NOA01_002588"
     CDS             1441..2871
                     /locus_tag="NOA01_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633736.1"
                     /translation="MDDIKEVFLEVLSRYIQDNLLEYQNNGKKYEFSFDIKNYNDSLH
                     VVFQYNKAGKIGITDVHSFQIFLNEINIEASKSHLNYTTILDTNSLFFSKKLLPYLHS
                     YEWALRKLIYLLAPTYFDNWTKDSIPDEMISAVKGKQKQAIGKYDLENLLQWIDLYDF
                     EEYLFGENYLFISTGNEKNIVRYKEIIVEELLNLFRETDDLSLSDPFSLWSEVFSKYM
                     YVDLEEIRKDMELIREGRNIVSHNKEIKYSLYKDLIKNLKKYVKQLNEAFKKILIGEI
                     ADEKLFDMTNDFEAYIGNHYSNINLPNATKLLTSLSAMTVLNTDSTKKMMETIGPALS
                     VQRAVMQNIDTEGFRRATEGMKQSLSAMTVLNADSTKKMMETISSALSAQRAVMQNID
                     TEGFRRATEGMKQSLSAMTVLNTDSTKKMMETIGPALSAQRAVMQNIDTEEFRRATER
                     MKQSLSPKQALKSDYKIADDIEDSNK"
BASE COUNT         1153 a          363 c          496 g          909 t
ORIGIN      
        1 aaacatttga aatagatcca gactattatc aaccacaaga agtttttcga gagtttagaa
       61 atcagattat tcgttcaagt aatattgaaa attgagtcaa taaaagaagc gttgaagaaa
      121 agttccttct ttcgacgctt ctttttaaaa gattttacaa atgtcttatg gagatttgct
      181 tcaatgacta cttgtagtca tttcgtttaa aataattatg ttacaccctt atgtgtcaca
      241 gagaaatact gatataataa gaaataaggt atgtaacaat agagtatact tctatgttac
      301 atattgatta ttttgaaaaa atcatattag gagaatataa ttatgagaag tgatagtagg
      361 tttttcgtca gtaatttaca agatgacgag ctaataaggc agattgattc attattagaa
      421 acaataacag aatctgataa aagaatattt ttaaattatg tagaattaac acgtcatatt
      481 atagagttgg ataaactttt taatgtgttt agatataatt taactaattt gctcaaacac
      541 ttcacaattt ttactaatga tttaattgaa agtacaggtg aaaaattaac ggaagaccag
      601 tattattatc agataaatgc tttgacgata aacttaatta gttcagccaa aactttgact
      661 gaatccattg aagtttgtat gaaaaacttt ttagcagaaa aagattttaa atcttttaag
      721 aataaaattc tgagtaagcc ttatgatgaa catttctcgt acagattttt attacatgtt
      781 agaaattatt ctcagcatgg tcacttaccg gtaaatattg aacaacaaag agtatacttt
      841 gatttggatg aaattctaac aatgcctcat tttgatctta atgaaaaatt aaaatcagaa
      901 atagatgaaa ttaaagaaga tatatctgct cgatttgagg attttccacg tatctcatat
      961 gtgtacacaa ttgcaaagtt taatctgatt acaactgaaa tatatttgaa ctatttaaaa
     1021 gaagttaaac caattttgat ggaaatggat aaagaaaaaa atgaattgct actcaatact
     1081 aaatttaagt taactaattc agatggaaaa tcaagtgatg tggtttttta tgactttgat
     1141 ggagagaatt atcattgttt caatcgaaca gacaatagtt tatcgatgta tgctagcata
     1201 aaaaaggagg ttaaaaagat attaagagaa gaagaacaat attataaaga aataaaaaat
     1261 aaaaaccagt aaatatcaca atcatcaata ttagtgaaaa attcatttgt ggttgttgtt
     1321 tgaggcttac ttagtagtta tcaagttatt tggaaaattt taaatcgttg aaagaaaaga
     1381 aggcgaggac atattagcaa gcaagttgtt gaatatttat attaccctaa acaaggcaaa
     1441 atggatgata ttaaagaagt ttttttggaa gtattatcac gatatataca agacaacctt
     1501 ttagaatatc aaaataacgg aaaaaaatac gaatttagtt ttgatattaa aaattataat
     1561 gattcgttac atgtagtatt tcagtataat aaggctggta aaatagggat aaccgatgta
     1621 cattcatttc aaatttttct aaatgaaatt aatattgaag cgagcaagtc acatttaaat
     1681 tacactacta ttttagatac aaactcatta tttttttcaa aaaaattact accatatttg
     1741 catagttatg aatgggcttt aagaaaattg atatatttat tggctccaac atatttcgac
     1801 aactggacga aagattctat tccagacgaa atgatttctg cagtaaaagg aaaacaaaaa
     1861 caagcaatcg gcaagtatga cttagaaaat cttttacagt ggatagattt atacgatttt
     1921 gaagaatacc tatttggaga aaattaccta tttatttcaa ctggaaatga gaaaaatatt
     1981 gtaaggtaca aagagataat cgtagaagaa ttgttaaatt tatttcgaga aactgatgat
     2041 ttaagtctat ctgatccttt ttcattatgg agtgaagtat ttagcaagta tatgtatgtt
     2101 gatttagaag aaattcgaaa agacatggaa ttgatcagag aaggaaggaa tattgtgagt
     2161 cataataaag agattaaata ctctttatat aaggatctga taaagaatct aaagaaatac
     2221 gtgaaacaac ttaatgaagc atttaagaaa atactaattg gtgaaatagc agatgaaaaa
     2281 cttttcgata tgacaaatga tttcgaagca tatataggga atcattatag taatataaac
     2341 ctacccaacg ctactaaatt attgacgtct ttatcagcga tgacagtatt aaataccgat
     2401 tccactaaaa aaatgatgga gacaatcggt ccggcattat ccgttcaacg agcagtcatg
     2461 caaaacattg atactgaggg gttcagacga gcgactgagg gaatgaagca gtctctatca
     2521 gcgatgacag tattaaatgc tgattccaca aaaaaaatga tggagacaat cagttcggca
     2581 ttatccgctc aacgagcagt catgcaaaac attgatactg aggggttcag acgagcgact
     2641 gagggaatga agcagtctct atcagcgatg acagtattaa atactgattc tactaaaaaa
     2701 atgatggaga caatcggtcc ggcattatcc gctcaacgag cagtcatgca aaacattgat
     2761 actgaggagt tcagacgagc aactgagaga atgaagcagt ctctatcacc aaagcaagca
     2821 ttgaaatccg attataaaat tgctgatgat attgaagata gtaataaata aagagtccag
     2881 aactttttcc aattaataaa ctattaaaag aattagagag g
//
LOCUS       DAIGOK010000191         2908 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.196, whole genome shotgun sequence.
ACCESSION   DAIGOK010000191 DAIGOK010000000
VERSION     DAIGOK010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.196"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(<1..330)
                     /locus_tag="NOA01_002589"
     CDS             complement(<1..330)
                     /locus_tag="NOA01_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepB"
                     /protein_id="HCI0633737.1"
                     /translation="MNKNITKARHFGFLLYPESIPENWREQLESLGVSMAVSPLHDMD
                     EKTNKKDWTSSDVIRNGKHYVKPHYHVLYIARNPVTIESVRKKIKRKLGNNSVAHLEI
                     IDHIKGSY"
     gene            complement(411..560)
                     /locus_tag="NOA01_002590"
     CDS             complement(411..560)
                     /locus_tag="NOA01_002590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633738.1"
                     /translation="MAESERLVVILPSEIKRKLEEKAKDKSLSMSTYVKLLVTEDLKK
                     SEKGN"
     gene            complement(1047..1226)
                     /locus_tag="NOA01_002591"
     CDS             complement(1047..1226)
                     /locus_tag="NOA01_002591"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633739.1"
                     /translation="MEKDIYKQLTRQIQKEKEKGLLDHLDELELKADEINKNRMSAKE
                     KGIKLKDSSLQSDKS"
     gene            complement(1351..1596)
                     /locus_tag="NOA01_002592"
     CDS             complement(1351..1596)
                     /locus_tag="NOA01_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633740.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLKYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1941..2225
                     /locus_tag="NOA01_002593"
     CDS             1941..2225
                     /locus_tag="NOA01_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633741.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            2228..2644
                     /locus_tag="NOA01_002594"
     CDS             2228..2644
                     /locus_tag="NOA01_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633742.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNQPKNHQKGTNKTVTSSNFDPTKFLKR"
BASE COUNT          882 a          583 c          433 g         1010 t
ORIGIN      
        1 atacgagcct ttgatatggt caattatttc taagtgagca accgaattat ttcccaattt
       61 tctcttgatt ttctttctta cgctctcgat tgttacagga ttgcgagcaa tataaaggac
      121 atgataatgt ggctttacat aatgcttacc gtttctgata acatcactac tagtccaatc
      181 ctttttattt gttttttcgt ccatatcgtg taacggacta accgccattg atactcctaa
      241 gctctcaagc tgttctctcc aattctctgg aatagattcg ggatatagta aaaaaccaaa
      301 atgtctagct ttcgttatat tcttattcat gatatactta cctcaattga acactatcag
      361 ttaagcggaa acggtgcgcc aacacatatg tttctgctct ttttttgcgc tcaatttcct
      421 ttctctgatt ttttcaagtc ttctgttact agcaatttaa catatgtcga catactcaaa
      481 cttttgtctt ttgctttttc ctcaagtttt cgcttaattt cactcggtaa aattactacc
      541 agtctttctg attccgccat gcctttcagc ccctttagtt aattgttaat atcatggtaa
      601 acttttgaca actgtattgt caatattttt ttctattttt caaaacggcg gtcaaaacgg
      661 cggtcaaatg gcggtcgaaa aaaaaaggct ctatccattg gtacagtaag gataaagcac
      721 tttttggcac aaaaattggc actcggcaca ctatgggggg gtcgtagtag cccccccaga
      781 ttttccaaat tccaaatttt ttccaaaaat tttccagcgc tatcgctcgg caaatcaaga
      841 ccgtaccttt ttgaaaaaag gcaccccaaa aactttccac tcgccgttaa ggcgtcaaga
      901 acgttcccta tggtcacctt cttgtgtttc cagcttttgg gttttgttta tagccgtcaa
      961 ctccttaaag cctcaaagag acgctaagag ggcttattag cctctctaag cgtctctaat
     1021 tcgatttacc gttgaccgca cgttatctag cttttatcgc tttgcaacga cgaatcttta
     1081 agttttatac ctttttcttt tgctgacatt ctgtttttat tgatttcatc agcttttagt
     1141 tctaattcat ctaaatgatc tagtaagcct ttttcttttt ccttttgaat ttgtcgggtc
     1201 aattgtttat aaatatcttt ttccatcccc catcgcctcc ttaaaagcta ttattattcg
     1261 atttaagacg ttttatttag tttttggtgt tatttcattg gtccaagcta aaaaactctt
     1321 agaagcgatt ttagagccat ttaaacccac ttaattatat tctccatgaa attcgctcca
     1381 tgcgtcaaaa gctgcgtctg aaattccgcc gtcttcgttc actagttgca ttaatttttc
     1441 caacgatcgt tttctttcgg gcgtcatatt tttatatttc aacaattttt cgtctgttaa
     1501 atcgaacttg attgcttgaa ttgcttgctc taatttttct aagttttgaa aatattcgta
     1561 aggaaatttt tccatttttt ctttctccag tctcacaatt tttctagaaa taaaatcact
     1621 tctttgccgt ctatactctc aaaaaaaatg tcgtttgaaa aactaaataa atttttgatc
     1681 gtgcattttt aaatttggtt atcgatcaca cgtgcagcac tcccccccga attttaaaga
     1741 gcataaggaa acgtggtatc actttcttac gaaaggtgct agataggctg tgcctatcac
     1801 gtttctttga aaaaaatttg atatcacttt ttcgccatcg tggatagctt atgtatagta
     1861 ttttggcaaa aaacagctat acaaatatag tatttgtatg gtatacataa attatacata
     1921 attgcaaaag gagatttttt atggacattt tagttcggaa tattgatcca aagtttgttg
     1981 caacaataga caaacgttgc caagaactca cgaataaaac aggagaaaaa tggagtcgaa
     2041 ataaatatct aaaagtttta atcgaaagtg attttgaccg tcaacttacg acttacaaaa
     2101 aagaccaatt cgatttattg ctcgataaat ttattgctat ccaaacagaa aatactcaaa
     2161 tattaagcga gtatgtctac acaaataatc aaatcattga aatgcttatc ggaggaatga
     2221 tataaccatg agaaaaactt ctgtccaaaa acatgttact ttgtacccta gtcatgttga
     2281 cgttttagat tccatcatcg aaaaaaatcc agatatcaat aactactcgg aagccattcg
     2341 ctttttatgt atgagttatg aacccgatac caacacgacc actaccagca atcaggcaaa
     2401 actaaatgct atgggaaaag aaatttccat catgactgaa atcatcgcag aattcgctga
     2461 tttatatcta aaagatacag gcgttcttat cggcacaaat tctaaagtat atcaagaagt
     2521 gaaagaaaaa atcgaagcta aactaaatcc atcaaatcga aatcaaccaa agaatcatca
     2581 aaaaggaaca aacaaaacag tcacttctag caatttcgac ccaactaaat ttctaaaacg
     2641 ataaaaaaca gataggagtt attcctatct gttttttatt tcccaatatt ctattttttc
     2701 attccaagca gttaacggaa gttttcgata tttattcgac gtgtttattc acgccttcaa
     2761 tcatttctcc agtttcagaa tctatccttt ttgcatattg cggccgaaat ccgttctggt
     2821 aattagcatc aaacatcata cggaaaagat tgctgtttga agctatgaca tcaacaacat
     2881 ctctttgatt agtgattcca tactcttg
//
LOCUS       DAIGOK010000192         2865 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.197, whole genome shotgun sequence.
ACCESSION   DAIGOK010000192 DAIGOK010000000
VERSION     DAIGOK010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.197"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            95..1054
                     /locus_tag="NOA01_002595"
     CDS             95..1054
                     /locus_tag="NOA01_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS1252 family transposase"
                     /protein_id="HCI0633743.1"
                     /translation="MTYTHLTTDELVMIESYYHQNIKIVKIAEYLKRSRTPIYNVINF
                     LKEGHTALEYYQQYKENKKRCGRRNIVLPKKQQSYIKEKIAQGWTPDVIIGRKEKPIS
                     CSVRTLYRQFKEKVFDESTLPMKGKRKPNGHQERRGKQAFKRNIVERETDYPTFKEEF
                     GHIEGDTIVGIHHKSAVITLVERLSKIIITLKPEGRKAIDIENTLNHWFGSIPRHLFK
                     SITFDCGKEFSNWKALSNQHDIAIYFADPGTPSQRALNENSNGLLRKDGLPKNMDFNQ
                     VDQAFISSVANKRNNIPRKSLDYQTPLEVFMSYMNEDILSSLI"
     gene            complement(1487..2059)
                     /locus_tag="NOA01_002596"
     CDS             complement(1487..2059)
                     /locus_tag="NOA01_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCI0633744.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2075..2680)
                     /locus_tag="NOA01_002597"
     CDS             complement(2075..2680)
                     /locus_tag="NOA01_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HCI0633745.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          918 a          581 c          471 g          895 t
ORIGIN      
        1 gccgattgta aaattaagct agacaaataa ataagccatt cgtgctacac tcaaaatgta
       61 attccgacta aagaaaacat aggagtgatc acgaatgacc tacacccatc ttaccacgga
      121 tgaactggtg atgatagagt cttactacca ccaaaacatc aaaattgtga aaattgctga
      181 gtacctgaaa cgatctcgaa caccgattta taacgtcatt aatttcttga aagaaggcca
      241 tacggccctc gaatactacc aacagtataa agaaaataag aaacggtgtg gcagacgaaa
      301 catcgtcttg ccaaagaaac aacagtccta cattaaagaa aaaattgccc aaggctggac
      361 gccagacgtt atcattgggc gtaaagagaa accaatcagc tgttccgtaa gaacccttta
      421 tcgccagttc aaggaaaagg tgttcgatga atcgactttg cccatgaagg gtaagcggaa
      481 accaaatgga catcaagaac gccgaggaaa acaggctttt aaacggaata tcgttgaaag
      541 agaaactgat tacccgacat ttaaagaaga gttcggccat atcgaaggcg ataccattgt
      601 aggcattcat cataagagtg ctgtcataac tttggtagaa cgtctatcga aaatcatcat
      661 caccttgaag cccgaggggc gcaaggcgat cgatattgag aatactctga accactggtt
      721 tggatcgatt cccagacatt tatttaaatc cattaccttt gattgtggga aagaattctc
      781 taattggaag gctttaagta accaacatga catcgctatt tatttcgctg atccagggac
      841 tccttcgcaa cgagcactga atgaaaattc caatggcttg ctgcgaaaag atggtctgcc
      901 aaaaaacatg gattttaatc aagtcgacca agcttttatc tcttcagtgg cgaataagag
      961 aaacaatatt ccacgaaagt ccttggatta tcaaacaccc ctggaagtat ttatgagtta
     1021 catgaatgaa gatattttgt ctagcttaat ttgacaaatc aaattattta aaactttgca
     1081 acagaacctt agcagcagtg ctgattttta tgatagttat ttggttctta aatgaaagat
     1141 ctaaagacaa tcaacaccgt agggatatgg agataaaatc aatagagtca atagattatt
     1201 tatcgataat aacactaaaa aagccacgtt cctaaacgtg acttacgctg aacgatatgt
     1261 gttgtttggt agacgaacaa atcgttcatt ataaatatat gatggtatcc taatatagta
     1321 agcgtggcta gcataccttt aattacttta ccttttacag ttaccataca cattttttga
     1381 gcagaaatca agtaaataaa aaaatattat atttcacaac atgctaatag actcatatct
     1441 agaacgcatt aagtatttac ctgtctggtt cgctgagact tcttttttaa ctttcttcaa
     1501 ttttcttctt tattctaaaa agcgtactgc gactgaaacc ggttatagct tcaacttcct
     1561 tatacgattt acctgaagta agcagttcat atgcgtgcct cttcttcggt gtgattgttg
     1621 ctttcggacg tccttctctg aaattaggat tattctttct tgcaaacatt tttccttctt
     1681 gggtacgttc cacaatcata tcacgttcca tttctgctac actcaacaac gtccgtgtca
     1741 ccattcgccc catggacgta ttttcaatcg ttcctagatt caaaaccttg atcgttacgt
     1801 tatcatcgag taaaggttca atgatctcta atgcttctct cgtgttacga gcaaatcgat
     1861 caagcttcgt aacaactatt tcatctccag gcttcagacc agccaacagc tgattgaatt
     1921 ttggccgatc ccgttttgta cccgtaaatt tttctgaaaa aatattcttc ttctctacgc
     1981 cgttctcaag caactgttga atttgttctt ttgtactttg agaagcggtt gagacacgtg
     2041 catatccata tttcgtcatt tggtccacct acttttacaa atcatctatc gtatactcag
     2101 aatacccttc atagaaatag ctgacatcga ttcctttcat atataactcg cgatcgtcaa
     2161 ttttatcagt cagagcattg gaaattagat agcgtatttc caaatcgttt atgggacttc
     2221 gctccattgc agaaagatag tctgctttat cgattaagtt ccaatctaca actttttgca
     2281 attcttcctt taaaatgaga tccagccaaa ttctagtact tcggccattt ccttctcgaa
     2341 atgggtgagc gatattcatc tccacatatt tcttgataat ctcttcaaaa gaagtttgtg
     2401 gcatctgatc aatatgctgt aacgaatgtt ctaaatacat aactggtgca aatcgaaaat
     2461 ttccttttgc aatattgacc gatcggattt ttccggcaaa atcgtaaata tccgaaaata
     2521 gaaattcatg aatttcagat aaacctttaa atgtaccaac ttcgatttca tttatttttc
     2581 cacaatcgta caattctttc gctttttgtt tactgagttt ttcttcaacc ttagccaact
     2641 caacttgatt ggttaatccc aatttattct ctaaaatcat gcgaaatctc cttaacttta
     2701 tgacataagt ttatgaaaca ctctcaaccc cattatatcg cgattttgtt tatgtgtcaa
     2761 aaactattaa gtttctgacc ctctacaaaa ttcacaatag atataatcta ttgaaaattt
     2821 aaaagaagac aaaatagagt acagaaaatg ttttctgtac tctat
//
LOCUS       DAIGOK010000193         2786 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.198, whole genome shotgun sequence.
ACCESSION   DAIGOK010000193 DAIGOK010000000
VERSION     DAIGOK010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.198"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(76..1170)
                     /locus_tag="NOA01_002598"
     CDS             complement(76..1170)
                     /locus_tag="NOA01_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCI0633746.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1359..2120
                     /locus_tag="NOA01_002599"
     CDS             1359..2120
                     /locus_tag="NOA01_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCI0633747.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="NOA01_002600"
     CDS             complement(2235..2675)
                     /gene="fabZ"
                     /locus_tag="NOA01_002600"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCI0633748.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
BASE COUNT          885 a          553 c          469 g          879 t
ORIGIN      
        1 cctataaaaa aacaaaataa aaaagcagaa ataactggct atttctagcg gattatttct
       61 gcaataacta attatctatt ttttattttc aattaattta aagcttgacc atggtttcaa
      121 aaagtcaagg aggaataatt cgtcatcgga gattcttcct acaacattga cacgtccgtc
      181 gtttttcata tccttcaagg cgatttgtgt ttctcctttg tattgaccgt acccttcatt
      241 gtcaatcagt acatccccat gatgcatatc tcgcgtatta tgaggcggga aatctttatc
      301 tttgtagtat acacgagtca tcgttgaacg aaggatatat tctgagcgat cccctcgata
      361 actatgaagg ttatcaaaca agcagattcg ttcgttttct gtaatacctt cttccgtatc
      421 tactttcaat gttggataat ccgcgttaaa tgcttctgcc atttctttca attcttcttc
      481 acttgcgtac gcatttccaa tagaaatatc atcaatcaat cctgtaagga ttaaatgctt
      541 cacttgtgtc gcaatttcaa gatctcggtg gtcttccagc gtgcacaagc catcttgcgt
      601 aggccaaggg ccaaactcag cagattgcga attgacgaat gccatcgtat tcaaattata
      661 ttttctgaat ttttctgaac aaaaaacaaa gtgttcgtat cctaaaccag agtatcgatg
      721 aggataaaaa ttatgagaac ctaataaatt atccgtgttt ggcgaataac tcatgatgtt
      781 gtcaacgtaa ttcgtgcccg aactcatatt gatctcgatt ttgataccaa aaggattgcg
      841 cgtcattttt gcttcttctg ctcctgtgaa tccgatatct aagcggaccc cgtaggcacc
      901 catcttatgg aaaaaagata agtcatcgta agaaatctcc aactgttcaa ataacgctgg
      961 attgatatca accattactt ccatgcctag gctattcgcg taatcaacta cttctttaaa
     1021 ttctgaaaga actttttctt tatcatcatt gatctgcagc aaactggtaa aaacccgttt
     1081 atacccatac ttatgagcta aatccaagta cgctttgtcc ttttcaaatg ttgaccgttc
     1141 tggataaatc gagattccta attttcccat attaccattt cctcctgcat cttttcgttt
     1201 ttgtatccac ttacattata gcaaacacaa agtaaatggt ctacaccttt tctttttctt
     1261 ttaaaataaa tgataaattc caataattta agtaggtatt ttccctctat tttgttatca
     1321 ttaaagataa caaaatagta aattggaaag gaacagttat gaagaaaaat aaagttattt
     1381 taagtattag tttagcagca gcattgctgt ttgctgctgg ttgttctgca aacaacacga
     1441 cttcttctag ttcgtctaca gaaagttcaa aaagtacatc tactgcggaa agcacgactg
     1501 ccagtagcgt cgatttgaac gcattggaat taccacaatt gacgaaagat gtcgccgaaa
     1561 atgaagatct ggtcgaaatg gtgacgaata aaggcacaat caaaatcaaa ttatttcccg
     1621 aacacgcacc aaaagcggtt gaaaacttta tgacacatgc aaaagacggt tactacaacg
     1681 gactgacttt ccatcgcgtc atcaaagatt tcatgatcca aggtggcgat cctaaaggcg
     1741 atggcactgg aggggaaagc atttggggag aaggatttga aacagagatc aacaaccacc
     1801 tttataatat aagaggtgcg ttatctatgg ctcgctcaca agatcctaac agtaacggca
     1861 gccagttctt tatcgttcaa aatacagatg atatgcatga tggtctttta aaagacgact
     1921 atcctcaagc aatcatcgac gcttataaaa acggcggtta tccttcacta gatggccatt
     1981 atactgtctt cggccaagtg attgaaggaa tggatgtcgt ggatgctatt gcaaatatcg
     2041 aaacagatag ttcagacaaa ccaaaagaag aagtaaaaat cgataaaatc aatatactgc
     2101 aagaagcaaa acaaaaataa aaaaataacg tctgaatcct gcatagatga caacgtatgc
     2161 taggattcag gcgtcttctt tttacataaa aagcaagtaa gcttcctttt gttttgctat
     2221 atcaggtttt atagtcaagc attttggttg acgatgaacg tgaactcgca ctcacagact
     2281 tttttatcat ctacatatgc tgcagataac gctgtaccga ctgatccctt cattttggtg
     2341 atttcaaaat gacatttcag cacatctcct ggaacaactt tttgacggaa tttggcctta
     2401 ttgatcccac cgatataagc tctttttcct agaaactctt ctgattttag gataagtatc
     2461 gaacctgctt gtgccagtgt ttctagaatc aacgcacccg gcatcactgg attgtcagga
     2521 aagtgacctt ggaaaaaatc ttcattgatt gttacatttt ttgtcgcaac gatctttttt
     2581 cctggttcta aagtatctac ataatcaata taacaaatag gataacgatt aggaatcaat
     2641 tccattactt cagtagcaga taatacgtaa gacataatta aactccttct ttcaggcaat
     2701 tgccattttc attactttga ctatcaaagt atcatattcc taaatattta gcaatctttt
     2761 tttaatttaa gaaaaaatag tttgac
//
LOCUS       DAIGOK010000194         2745 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.199, whole genome shotgun sequence.
ACCESSION   DAIGOK010000194 DAIGOK010000000
VERSION     DAIGOK010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.199"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(943..1356)
                     /locus_tag="NOA01_002601"
     CDS             complement(943..1356)
                     /locus_tag="NOA01_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HCI0633749.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="NOA01_002602"
     CDS             complement(1812..2297)
                     /locus_tag="NOA01_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633750.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="NOA01_002603"
     CDS             complement(2436..2651)
                     /locus_tag="NOA01_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HCI0633751.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
BASE COUNT         1020 a          454 c          418 g          853 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaa
//
LOCUS       DAIGOK010000195         2682 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.200, whole genome shotgun sequence.
ACCESSION   DAIGOK010000195 DAIGOK010000000
VERSION     DAIGOK010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.200"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            52..375
                     /locus_tag="NOA01_002604"
     CDS             52..375
                     /locus_tag="NOA01_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633752.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            539..793
                     /locus_tag="NOA01_002605"
     CDS             539..793
                     /locus_tag="NOA01_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633753.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            863..1108
                     /locus_tag="NOA01_002606"
     CDS             863..1108
                     /locus_tag="NOA01_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCI0633754.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            1184..1549
                     /locus_tag="NOA01_002607"
     CDS             1184..1549
                     /locus_tag="NOA01_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633755.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(1610..2173)
                     /locus_tag="NOA01_002608"
     CDS             complement(1610..2173)
                     /locus_tag="NOA01_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633756.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT          788 a          474 c          422 g          998 t
ORIGIN      
        1 aatttttatt tgcttatttt agaaaaaatg ttattctttt atttattctc aatgttagga
       61 gtgattattt tgccatttat tgcgatttta ttcgatttag ttgcggcagc agcttatttt
      121 ctccaattaa attatcaaac ctcaggaatt ctgtttattg gaatgatttt tcaaggtgtg
      181 attacccttt tgcttcttgt catgatgctc agctataaag gaaaaagata tgcccgtatt
      241 caaacaaaag tatttgttaa atatgtttcc attcgctatg ggatcatcat tctttccttt
      301 ttagtgaatg cgatcgtcct ctttttatat gtattgaatt acttagatat caatccattg
      361 attttttctc gatagataag acacattcag cggaacagtt cctgcttatc agcagaaaac
      421 tgttctgctt tttcattttt cattgatcta ttcttctttt attttcctag catttcattt
      481 gtgatgtatg caataacatg ttatctttta ttatatgaat cgttgaggag tccgttacat
      541 gattacgttg gtgagagttt tgttttgggt gcctgcggtc gcactggtcg caagtattgt
      601 ttatcttatg aattggaaca aagaacggtt ttatttagct attttgactc ttccggctat
      661 ctactttatg tggaaagttt ttaattacaa ctactttgaa cccgatagtg tttttataga
      721 agaattatca ggactggttt tgtctttatt gatcgtcatt ctttatttga tacgtttaaa
      781 taaaaaacat tgagtactat tgattgcatg tgtagctttc tatagtcatt ataactattc
      841 tactataatg aaaggtgatt aaatgagtat cgaagaagct attttactgg atactttacg
      901 ttgtcgtctg aatttcttaa gaactggccg atctttatct caagtagctg tttctagcgc
      961 gttaagtatt cctttattca aatatcgtaa gtacgaagaa ggagaagaaa ttccaagcat
     1021 cattgaatta atctatatcg ctgactttta cgatatttcc gttgattacc taattggtcg
     1081 ctcagaaaca gcaagcaaac tgaagtgatt ttttccagcc ttggtgctgg ttttttcatt
     1141 taacgtctaa ggttaacaat ctatatataa aggagacatt tctatgcgat tatggcatga
     1201 aaaattgatt tcaaagcttc ctcgtcaaca gcttttagga caacatcggg aatgctgtgc
     1261 attaagagga aatggctggg gaaagaaaca tgctactgtg gattatgttt ttcactattc
     1321 tccttataga ttattccaat atcatcagct tgtcatggat gaaatggaaa aacgtggtta
     1381 cagcccagca ccagagtgga aaaatcctat gtatcgtggc aaatcatgcg aagcattttt
     1441 tgatttacct actgtagagc gcaacgtgcc gatttatccg gaacacgatg aagtttatct
     1501 agcagaatgt ttagcaaatt tggagcaaaa aggaatttat cttacctaat ataactagaa
     1561 aagccttcta tctcctctta cttcattaaa aagctttaac catcttagat caagctaaaa
     1621 tgtcgtaagt tttagggtta tcttcatcct cacgtgcaaa actaaaagcg tactctagtt
     1681 ttggttcatc gccaacttcc ctagacataa atgaacatct cctgacgtgg tttaaaagta
     1741 ccgcatcttc gacgtctgta tacgatgcac agcacagatt gcttcttttg atctttcttt
     1801 tgatttttct gttgcttctt ctacaccttc ttcgattact acatcgatgc ttacaattgc
     1861 ttctgcctaa tattcccatg catcgtcgat ttaagcctct gtagtcactg tgatcgtttc
     1921 cacattttaa ctcctctgtc gtggcttcag ttgattttgt gatcagttca gttgctaagc
     1981 cagagtcaga acaacctgcc attgctagcg ttaatattga tagtaaaaat agttttttta
     2041 tcataaggtt ccccccctga aaatagacta aaataataac gctttctttt tttgttttca
     2101 gacgaaaatt aataatttct aaatattctt taaaaaagct tataactatt atgccttttt
     2161 taatatataa cacagttatt tttaaaaata agaatgctaa atcactctac aataacggcg
     2221 aaagcacaaa aaataaaaaa attattacgt tattaaaaaa acagatgaaa gatcaggttt
     2281 tattagatct cattctttca tcacttgaag ggatttaatg aactactttt tcctctttgt
     2341 cgtttttata tattcgtatt ccttcaaaca gtaggatagc ctctttcaat gcttgatatt
     2401 tgaactgtcc gttacttaaa aatttagatg tgccgccctc ttcctagaac atattctttt
     2461 ttactttttc attaattgac agaccaatcg aacgaaactg taaatttcga taggataatg
     2521 attggtcaaa ctcattcatt ttcaagacat ctcgattcat attcaagata ggaatctttt
     2581 tatcaacatc actttattcg tattttcctc taccataaaa gcttcacgct ccacaaaatt
     2641 gttttataca ctactattca gcccattcaa cgaccaatga at
//
LOCUS       DAIGOK010000196         2597 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.201, whole genome shotgun sequence.
ACCESSION   DAIGOK010000196 DAIGOK010000000
VERSION     DAIGOK010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.201"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..1152
                     /locus_tag="NOA01_002609"
     CDS             <1..1152
                     /locus_tag="NOA01_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HCI0633757.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="NOA01_002610"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="NOA01_002610"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCI0633758.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
BASE COUNT          862 a          491 c          621 g          623 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaa
//
LOCUS       DAIGOK010000197         2539 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.202, whole genome shotgun sequence.
ACCESSION   DAIGOK010000197 DAIGOK010000000
VERSION     DAIGOK010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.202"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..290
                     /locus_tag="NOA01_002611"
     CDS             <1..290
                     /locus_tag="NOA01_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633759.1"
                     /translation="ETQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINLYYSNK
                     NYFKIPAAKTRMKKDVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(803..876)
                     /locus_tag="NOA01_002612"
     tRNA            complement(803..876)
                     /locus_tag="NOA01_002612"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(840..842),aa:Ile,seq:cat)
     gene            complement(1065..2084)
                     /locus_tag="NOA01_002613"
     CDS             complement(1065..2084)
                     /locus_tag="NOA01_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HCI0633760.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(2095..2301)
                     /locus_tag="NOA01_002614"
     CDS             complement(2095..2301)
                     /locus_tag="NOA01_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCI0633761.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
     gene            complement(2434..>2539)
                     /locus_tag="NOA01_002615"
     CDS             complement(2434..>2539)
                     /locus_tag="NOA01_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCI0633762.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
BASE COUNT          778 a          484 c          422 g          855 t
ORIGIN      
        1 atgagacaca attatggttt aaatttgacc cttcgaatag atttatcaaa gatttttata
       61 aagtatggaa ttcagaagtt ttctttttag caatcgaaga tagcttatta atcaatctct
      121 actattctaa taagaactat ttcaaaattc ctgctgcgaa aactagaatg aagaaggatg
      181 tatacttttt gtttgatatc gtgactgacg tgccggacgc acgtagcgat catcggcgtt
      241 atgactatat aaagtatact ttcgttgatc cagaaagata caaagattaa agtaggctac
      301 ctaaaaaggt agcccggaac gaattttatc accatacttg tgaaaggaga tattttttaa
      361 gttagtatta agattgtgta atatgatgat atctatattt tatagtatca gtgctataaa
      421 atcaaaaata agtcactaat ttactaccac tccaattgta agcctttttt ctcacttttt
      481 ttcaaaaata tggtatgcct tttaatagct tcaaatataa aagagtttaa agcgtaacac
      541 acttatgggg aagtggcttg gggtgcgctt taaactcttc tttattatca tctcacaatt
      601 taacccaaat gtctttctat ttaaaaatca aagtaaaact tttcaaatat acagaaatat
      661 aactatgtga aacatccttt cattaatcca taaaaggata cataaaaaag ccactcattt
      721 gagtggcaat gaagaaaagc tttagcttgt ataatactct tcaaaaaatt ctaacacaga
      781 acgattcaaa tggctacgtt aatgtaccct gtaggactcg aacctacgac cggacggtta
      841 tgagccgtct gctctgacca actgagctaa gggtactggt tgttgccaca taaagccata
      901 aacaatcaac cagtagaatg tgtggcaaca aacctgttat cgcatagctt ggagtgtgac
      961 tattatgggt gatagtgaag atatgcgata acatcactat tttatcgaat gatttttata
     1021 gttgtcaata tagttatgta atgctcctca acgaggagct atttttatcg tttaggaata
     1081 tttaaatacc aacgtttgtc atggaaatct tgcgcaccgc ctttagtgtt tccctctgga
     1141 tcattcgttg cccgcatcat gacatagact ttcttattag ggaagttacg catgttaaaa
     1201 gatacatgat agccaacatt tccagaagta ttataagctt gatttacatc tgatctataa
     1261 attccatcag ctcttactcg agctaattct ttcccagtat tgtaatccat aatgaagata
     1321 tactcgtatt tatagttagc aatgtgccat ccagctacat gcaaatttgc attttcgatt
     1381 tctccaaact gatcaatgtg ggcgtaattc gttccatctg tcagtgtagg attagctgca
     1441 ccagctcgtg ttggatcaat gacaggcttg ttttcagaag ttgttggatt ttcatcggta
     1501 aatccatgag ctaaatcata tgctaatttt tctttactta cgcccatttc agaaagataa
     1561 ccgtaaggat ctgtatgatc gccccagatg ttttgtgtta cccataaatg cgatttgatt
     1621 cccggttggt tataaggagt gtccaacgtt aatggaatac catatttcat tgctgaatct
     1681 ctagccaatt caacgtatgc cttgtagttt ttctcaaacg ttgctttatc atgtgtgtgt
     1741 tgtaactcaa tctgcacagg actgttggca ttagcatacg aaccagcacc gtactgtaca
     1801 taaccaggtt gaccgacttg ataaacaatt ccgccgtctc ccacaatata agcagtataa
     1861 gcgctagtcc atgaacgttg catatactgc gcttcattgc gtcctgttgc tgtttcatta
     1921 gccgtttcat gcagtaaaat atactgatta tttgctactt gtgagctacc ttcatttgcg
     1981 cccaaattaa attcattgtt gatagtatag gcaaatccgt taattggcaa taaaaaaaga
     2041 gccgttaaca ggctcatcgc agtaatagta attttctttt tcatatctat cctcctattt
     2101 atctgatgaa attttcttat acgttcgatt tgatacacct aaaatactcc ctagaaatgc
     2161 tccaaaacct gtaataatca tcaccgttat atctgtgtac cgccagttaa caccttttcc
     2221 gacaagaccg acaaaagtcg ctaaagcggg gataactact atagcgatcc atttcaaaat
     2281 atcaaaggtt ctattgttca tactatccac ctcccttcat tggttgggct actttctttt
     2341 tcaatcgctt gttttcttct atcaatattt ctctttctga aagcaaggca tcaatttgcc
     2401 cgtccagttg ttcaatttta tctttcttaa ttgtcaaatt aagttagaca gatcatcaat
     2461 acttacgtaa ctcaaaaata cttccaaagg tgttcgataa tccagtgatt ttctaggaat
     2521 attatttcgt ttagatgcg
//
LOCUS       DAIGOK010000198         2461 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.203, whole genome shotgun sequence.
ACCESSION   DAIGOK010000198 DAIGOK010000000
VERSION     DAIGOK010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.203"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            224..2377
                     /locus_tag="NOA01_002616"
     CDS             224..2377
                     /locus_tag="NOA01_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HCI0633763.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          849 a          421 c          538 g          653 t
ORIGIN      
        1 caaagtgttt cacgacttaa gaaaacataa ttttcaaaaa taagtttatt ttttaatgaa
       61 aaaaatagtt ggatatgtaa ctttaagtga ttgctgtgaa acaaaaagtt gattttatct
      121 tgaaattagg taaagaattt cttgctccgc ccattttttc ttttttatta agaagttttt
      181 gggtacaatg aagcatgaat gtaaataggc ggaaggatag gaaatgatga aagattttat
      241 gaaaaaaata ttaaatactt cttggttaga aaaaataaga aaaggtgcac aagaacctaa
      301 gaacaaaacg tttcgccggt cccatgttcc ttttcgctta aattttcttt ttttcattat
      361 atttacgtta tttgttgcgt tgattgctag attgggttat ttgcagatcg tgaacggcga
      421 agagatggag gcgcgagtga agtctacttc tacgattacg gtccaagaaa gtactcctcg
      481 cggaatgatc tacgactcca caggaaaagc tttagttacg aataaagcga atcaggccat
      541 cacatatacg agaggaacac aagttacggc taaagatttg cttgaaatcg caacgaagct
      601 gaactcgctg atcgatgttc cggtagatga aaatctgaca gatagagata aaaaagattt
      661 ttggctggct gatcccaaac atttgaaaga agccacaaaa agactttcgg caaaagaaaa
      721 gcaattaggg aacagcgaac aatatagtgc gatggtcaat aaagtcacag ctgatgagat
      781 taatttaaac gaggagcaat taaaaattgc aaccatcttt aaacggatga atagtgcata
      841 tgaattaaac actgtctata tcaaaaactc tggtgttact gatcaagagt tagctgttgt
      901 ggcagaacgc gcttctgaac ttccaggagt ttcgactggc acagactgga cacgagaata
      961 taatgcagca agttcactga aaagtattct tggcagtgtg acgactgaaa agcaaggact
     1021 tccagcagat gaagcagaga aatacctagc aaaaggttat tctcggaatg accgtgtagg
     1081 acaaagttat ttggaaaaac aatatgaaga tgtcctgcaa ggaaccaaaa cccaatatga
     1141 agtttccctt gataatgaag gaaatgtttc gaatcaaaaa gaaatcttct ctggggaaaa
     1201 aggttctaat ttaatgttat ctatgaatgc agaattccaa tcaaaagtag aagaaatttt
     1261 gaaaaggaat tatcaaacat tgatcaacaa cggcaaagca cagtattcgc ctggtgctta
     1321 tgcagtggct atgaatcctc agacaggaga agtgctagca atgactggtt tcagtcatga
     1381 acaaggttcc aaagaaatca ccgagaatgc tttaggcacg attactagcg cctttgatcc
     1441 agggtctgta gtcaaggctg gtactttgac agcaggctgg gcaagtaacg cgatcagcgg
     1501 aaatcaagtt ttgattgatg agccgattcg tctgcaaggt gcttcagaaa aaagctctgt
     1561 ctttaatcga agcggacaag ttgctcttga tgcagtcaaa gcatcggaat tgtcttctaa
     1621 tacgtatatg atcaaagtgg cgttaaagat gttaggacta gactatacac ctggaatggg
     1681 actacctagt ttagatgaag aagctaaagc ttatcagcaa ttgagagact ctttcaaaga
     1741 gtttggtctt ggtacaacga ctgggatcga tttgccgaat gaatctcctg ggatctcacg
     1801 ttctgttgat tatatgaaga agttcaatgc ggataatggc aaagaatggt atgctccagg
     1861 taactttacc gatttggcat ttggacagtt tgacacatat acaccaatcc aattagctca
     1921 atatgcttca acagtcgcta atggcgggaa acgagtacag ccgcacctag tcaaagcgat
     1981 ctatggcaat gacgaaaatg gaaacttggg tgaagtgaag aaagaaattg gcacaacagt
     2041 agaaaatacg gtcaatattt ctgcagaaaa tatggcgatc ctccgtgaag gattccacca
     2101 agttgtccat ggtaccgatc cttacacaac agccaaacca ttagccagtg cgaaaatgga
     2161 cctttccgca aaaacaggga ctgcagaaac ggttgcggaa ggtcatccag atattgcgac
     2221 agtcaatagt aatatcgtgg catacggacc aacagacaat ccggaaattg ccatcagtgt
     2281 tgtcttgccg aacttattag acgaacaaga ccacataaac ttgacgattg ctaaagaaat
     2341 aatggacgcg tattatgata tgttcatggc aaaataaaaa agaggtcgtg aaaaagccgc
     2401 ttagtttcga gtatgaagaa aaattttgtg aaaatagctt ctcacatttt tgcttaaatt
     2461 t
//
LOCUS       DAIGOK010000199         2401 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.204, whole genome shotgun sequence.
ACCESSION   DAIGOK010000199 DAIGOK010000000
VERSION     DAIGOK010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.204"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            207..2069
                     /locus_tag="NOA01_002617"
     CDS             207..2069
                     /locus_tag="NOA01_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCI0633764.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..2401
                     /locus_tag="NOA01_002618"
     CDS             2069..2401
                     /locus_tag="NOA01_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCI0633765.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          867 a          353 c          466 g          715 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataatta
     2401 a
//
LOCUS       DAIGOK010000200         2334 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.205, whole genome shotgun sequence.
ACCESSION   DAIGOK010000200 DAIGOK010000000
VERSION     DAIGOK010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.205"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            52..570
                     /locus_tag="NOA01_002619"
     CDS             52..570
                     /locus_tag="NOA01_002619"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCI0633766.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            698..1381
                     /locus_tag="NOA01_002620"
     CDS             698..1381
                     /locus_tag="NOA01_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HCI0633767.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1690..1763
                     /locus_tag="NOA01_002621"
     tRNA            1690..1763
                     /locus_tag="NOA01_002621"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1724..1726,aa:Arg,seq:tct)
     gene            1922..2281
                     /locus_tag="NOA01_002622"
     CDS             1922..2281
                     /locus_tag="NOA01_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HCI0633768.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
BASE COUNT          789 a          355 c          467 g          723 t
ORIGIN      
        1 atcgttatta tatgattaag aaggattgaa taaagaaaga agttgaaaac gatacctgat
       61 tacaaaaaaa taacttttat aagttctata acgttgatta tatttttaat tcttattctt
      121 ctcgtcgtct tggttccccg aatttttggt ttgactcctt atattattga ggacaaagaa
      181 atgagtccat attatgcaca aggaaatctg atatatgtac gaccgaaaaa tacaaatgag
      241 attttagtcg gagatattat tacatattat gaaaattcag gaaaaaaaat ctcgacgcgt
      301 cgcgtaatag ccgttgaaaa caaccacgaa gatttttata caaaagctga tacaaagact
      361 tatattgagc ctggagtagt aaaaaagaga aatattatcg gtagtcccat ctttcagatt
      421 ccatatattg gactagtcct ttcaaaaacg gcatttgcat ggattcaggg gttattcttt
      481 acgattgctt tttgcctaac aggaatcaca gcatggaata cctttgtaga gttgaaaaag
      541 aagcggagac gaacacagaa atttttgtaa gaatgttaaa aacataaatg tattccagag
      601 atccaacgac tcttctgaac atgaaacacg agaaaacact catgcagctt gtagatttag
      661 tcagttgttc gtataatgtg tgaagaggag gagtagtatg cgcaatgaaa tgatgcatcg
      721 tcctgtagtc aaagaagaac tattggattt tatgcgtacc cagcaaaaaa aattaccggg
      781 tgagctaggc aaactggaag aagaagctca tgtagcagaa gtaccagtta ttccccatga
      841 aacagttgta tttttgaaat ttttacttgg acagcttcag cctgaacgaa tcttggagat
      901 cggagcagct atcggttttt catcaagtgt catggccacg acaatcggag aaaacgctca
      961 tgttacgaca attgatcgtt tcgatgtcat gatcgagaaa gctaaaaaaa actatgaacg
     1021 tctaggactg acagataaag tgactttgct tgaaggtcaa gcagcagata tcttgccgga
     1081 gttgtctggt ccgtatgact ttatttttat ggatagtgca aaatcaaaat atattgaatt
     1141 tttacctgaa tgcttacgtt tgttaagaaa aggtggcgtt ttgatggtag atgacatctt
     1201 ccaaggagga acgatcttgc tcccagatga agagattccc cgtggcaaac gagctatcca
     1261 tcgtaaatta aatgaatttc ttcgtgttgt gatggatcat cccgatttga catcgacaat
     1321 cttgccatta ggtgatggtg tgatcttaat gacaaaggaa tcagaaacaa tcgagttgtg
     1381 atacatgatt atggcagcct gtagataatc gattgaatcg agggctcaat tcgaacttga
     1441 tgtaaaagtt tctatatttg taagtcaatt aaaggataca ggaaatctaa ataaacagga
     1501 gtagcatgaa gtcaatgtag ctcctgtttt caattgataa ccttagaaca gctgaaggag
     1561 aatgtctcgt tttaaaagat tgatttagta gttgggccaa aataattcat tatttgctaa
     1621 ttaaataact atttcgtatt gacagaagca atttaattat ctataattat tgatgttccg
     1681 ctgattcttg tcatagtagc tcagctggat agagcatccg ccttctaagc ggacggtcgg
     1741 gggttcgaat ccctcctgtg acgctaaaaa accgttagaa actgaggttt ctaacggttt
     1801 ttctttgttt tttgtgccaa tattgtttgt tttcaaaggc aaataaaata aaaaaacatc
     1861 cttatcgagt acaattataa ggagcaaaaa aataaaactc atcagaaaag aggaagaaga
     1921 tatgacgata ctatctagaa ttttagtgac tctagttgca attgaatttt tttatatcat
     1981 gtatatcgaa acggtgagaa ctgattctga cacgacaagt agggttttta aaatgagtaa
     2041 agaagagtta agcagaaaat ctgttcaaac attgtttaag aaccaaggag tgtacaatgg
     2101 actattagga gtgggactac tttatggagc ttatctttct tccgcatcta aagaaatcac
     2161 aagtatgcta ttaatatcta ttttttttgt tgcattatat ggcagtttga ctagcgatcg
     2221 ttggatttta ctcaaacaag gtgggctagc tctgttggca ttgctttcta ttttcttata
     2281 aatatagaaa aaataagaat ttatctaaca ggaaactttg tgaaaaatat tact
//
LOCUS       DAIGOK010000201         2286 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.206, whole genome shotgun sequence.
ACCESSION   DAIGOK010000201 DAIGOK010000000
VERSION     DAIGOK010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2286
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.206"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(1014..1304)
                     /locus_tag="NOA01_002623"
     CDS             complement(1014..1304)
                     /locus_tag="NOA01_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633769.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(1512..2204)
                     /locus_tag="NOA01_002624"
     CDS             complement(1512..2204)
                     /locus_tag="NOA01_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCI0633770.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT          653 a          433 c          334 g          866 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt
     1501 gttaagaaag attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta
     1561 tcttccactt catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt
     1621 tcagcagttg cttcgtctaa tttcttttgt gtatctgtta actgagtttc tacatttgtt
     1681 ttttcttgtg tcagtgttgc gatttgttga ttcaaaccat ttacagtatt ttgtaattct
     1741 gaaagttttg cttgtgcttc ttgtagtctt gctgtccaat ctgcattcag atgattgatt
     1801 tcatcatttt tttggttgat ctcatctagt ttgacattga tttcattttg tttgctttgt
     1861 agttgtgcat ccttgctgac taattgactt tctaagtttt gtttttctgc tgccgattga
     1921 tttttttggt tttctaattc atttactttt gtttggtatt cttggacatc tgcctttgaa
     1981 tcttgcaatg tctgttcttt ttgtgctagt cgagcggtaa gatctgtaat cgcttgctct
     2041 ttatctgtca atgtatgatc aagctgtgtc agattctcat tgattgtatc aatatctgtc
     2101 tctccacccc acaaaataat ttcatttgcg taccgcgtgc ctgtgtagcc aattagacat
     2161 agaaatgcaa cgacaatccc accagtaata aatttctttt tcatttgcat agctccttaa
     2221 tccaatactt ttacatgcta aaagtaagtg atctcaaaca caatgtcaag cgtttttaaa
     2281 ttacat
//
LOCUS       DAIGOK010000202         2225 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.207, whole genome shotgun sequence.
ACCESSION   DAIGOK010000202 DAIGOK010000000
VERSION     DAIGOK010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.207"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(366..1658)
                     /locus_tag="NOA01_002625"
     CDS             complement(366..1658)
                     /locus_tag="NOA01_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCI0633771.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(1932..2192)
                     /locus_tag="NOA01_002626"
     CDS             complement(1932..2192)
                     /locus_tag="NOA01_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633772.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          728 a          425 c          344 g          728 t
ORIGIN      
        1 gtatgggcta ctctgtgata cttattagcc attaaactat tttacccaca tagcatatat
       61 tttgaaatcg ttcattccgt aaattcaact aaatatgcct aaaaaaataa aaaatctaag
      121 atagaataag atttattcag tcttagatgg atttatagca actttttcgt aacaacttta
      181 aaaaatacga aaagaaagag ctgttttact ctgactacgt tgctaaattg tcgtttaatc
      241 attcatttaa accaaatctt tttttataga attcgtttta atcctcctga atccaaaaac
      301 tatgaaaacc gtaattaaat agttaagagt ttttaaccac cataattctc tcggctattt
      361 tgaaatcaga taaattttga tcggacacat agttgaataa acctaccgtt gttacggaag
      421 gtaaatcgca tacttttcat tctagaggag aaggtctcgt tgatcattaa ctgattaagc
      481 accatagccc agttttgaat tcgaccacct gaccacttcg cgtgtaattc tttcgtgcgt
      541 aaataaagta gttttaggag tgcattctca ttagagaacg ctcctttttt tgtgactttt
      601 ctgaagctgg agtggacact ttcaaccgca ttggtagtgt acataatttt tcgaatggca
      661 ctaccataat caaatagttg ttcaacatgt gcaaagttcc gtttccagac atctacagca
      721 ccagaataat gagaccaccg attttgaaaa ctgtcaaaag cagcatgtgc agcgtttaga
      781 gaagaagcac cgtagaactt tttcatatct cggcagactt ccttatagtc cttacttgga
      841 atatagcgca atgcatttcg aacaagatga acaatacagc gctgaacaat taccgatgga
      901 aagatcgctt ttgcgccttc ttcgaggcca gaaacaccgt ccatcgaaat gaaaaagaca
      961 tcttcgacac cacgtgcttt cagttcatca aatacttgca tccagcgatt tttagattct
     1021 gtttgattta accataatcc taaaatctcc ttatttcctt tgagatcata gccaagaatg
     1081 gtgtatacag catattcttt ggcttcataa ttttctcgta aagtaacata catacaatca
     1141 acgaatagaa aggcataaca ctttgctagg gggcgggctt gccattcttc caattcagga
     1201 aggacagcgt cagtgatatc tgaaatcatt tcatgggaaa tatcaaagcc ataaatagct
     1261 tcgacggttg cggcaatatc tcgttgactc attcctcgtg catacatgga aagaaccttc
     1321 ccttcgatgt cggagacatc tcgttttctc ttaggaatta actctggttc aaaggaagct
     1381 tcccggtctc taggaacatc aatagctact tcaccaaaac tggttttaag cgttttagtt
     1441 ccatagccat ttcgacggtt atcgtgttcc ttaggctctt tagaatgggc atcataacct
     1501 aaatgattat tcaattctcc ttgaagcatt ttttcaaaaa ggggcccaaa cacatctttc
     1561 aaagcatctt gcatgtcatc gacagattca ggttgatagg cattcagaat ggattcagct
     1621 aacttttcgg catcaggatt tcttttcttt ctagccatcg tgtaatcacc tcttgatctt
     1681 attgtagaaa aagaaaaacc gcgaagcaac cacctgccta ggattaatgg ttacttacac
     1741 ggtttatatt acactctcat cattaaaaaa gtatctttcg atattactaa acaatgaaat
     1801 tatctatttg aaatttaaat ctgataatat attgagtgtt ccaatgaggg ctttgatcct
     1861 attatccata tctttttcag ttttaatatt gatacacatt ataagaaaaa gaaacttttg
     1921 gttaatttat ttcatcccag tgcaaaatat aaaaaagtaa aatagaccat aggcaataga
     1981 ttaacaatta atataataca agcaaatatt ctattcattg tatttctgct ttcaaaaagt
     2041 tctaatattg aaagtataat tccacaaatg cctataataa gtatgggaat aagattagaa
     2101 aataacaccc cgagccaatc tgtgaaaatt actatattta ctaatatccc tattaacata
     2161 cctatcatat ttattaaatc aatttttttc attagatatt tctcctttat taaatctgaa
     2221 gtact
//
LOCUS       DAIGOK010000203         2198 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.209, whole genome shotgun sequence.
ACCESSION   DAIGOK010000203 DAIGOK010000000
VERSION     DAIGOK010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.209"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            62..>2198
                     /gene="acm"
                     /locus_tag="NOA01_002627"
     CDS             62..>2198
                     /gene="acm"
                     /locus_tag="NOA01_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HCI0633773.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAG"
BASE COUNT          859 a          346 c          432 g          561 t
ORIGIN      
        1 ggatttttga gagatgatat agtaggtttt atttttagtt aaaatagggg agatagaaaa
       61 aatgaaaaag tgtagaaata ttattttatc aatgttattt attattacta atataacatc
      121 actgattcct gttcatgtct atgcggatgc aggcagagat atcagcagta atgtcacttc
      181 gttgaccgtg gacccaacga atattacaga tggaggaaac ataaaagtaa aattctcgtt
      241 tgacgaaaaa aaacaaaata tccaaccagg tgattatctt tggatcaatt ggccaagtga
      301 aggaaatatt cgcggtgaag gattccaaaa agaaatccca ttaatgattg aaaataaaaa
      361 tgttggaact ttaacggtca gaaaagattc tgcgcaggtt gtgtttaatg aaaatattaa
      421 aaacctagac tcagtagaag gttgggggca atttgagata caggccagaa acgtaaccga
      481 taccggtgaa gaaaatacgg gaccattcac cgtgacgagc ggtgataaaa cagctactgt
      541 aaatgtgaca aagccagctt ctggttcttc ttctagcgtt ttttattata aaacaggaga
      601 catgctacct gaagatacta agcacattcg atggtttttg aatattaaca ataacggcac
      661 ttatgttgaa caaccagtta aaatcagtga tgaaatccaa agtggacaaa gattagatcc
      721 aagtacgttt gaaattaatc aaatacactt aggtgaacag aaggtttata gaggagaaga
      781 gggaatccaa caatttttac aagatttccc cagcgctaca tttaattttt cagtaactga
      841 taattacatt gaaataacta taccaaaaaa ctttgtcaat cttaggaaaa taatggtgag
      901 ttataagacg atcatagaaa acccagaaca aataaatttt gagaatcatt cggaagcttg
      961 gtttaaagaa tttaataaac ctgctgttga tggagaaagt ttcaatcata cagttaaaaa
     1021 tatttcagct tctggtggag tcaacggtac agttagaggc gaattgaaga ttttcaaata
     1081 tatcaatgac acagagattg gtatcccaaa tgtgactttc gaattaagac gagcagatga
     1141 acagcctatt caaggacagt ctagtatcct gttgacttcg aatgaacagg gagaagctag
     1201 tatcaaaggc ctgcaagtag gagactatgt tgtaaaagaa aaagaagcac ccaattggat
     1261 tgattttgat ccactaagtt caaatgaact caaattctcc ataaacgaaa atgatacaga
     1321 gggagtcagt ttacctattt ataacaagaa aaaggttacg aacatcacag caactaaaat
     1381 ttggaacggt ggaaccactc caagaccgag tatttatttt aaattattca gagcaagtac
     1441 aaacaattgg gaaccagttc ctgatgcaga aacaaaacga ttagacaatg gaataacttc
     1501 agtgacttgg gaagatatcc aacagtatga tgatagtgga aacgaatata cgtttaaagt
     1561 gcaagaagta gatcaaaacg gaaatgatta tgtcccttca ggttatcaaa aaattgaaaa
     1621 cgggctagtc gttacaaatg agaataaaga agtcatttca gtctcaggac aaaaaacatg
     1681 ggaagacaat gagaaccaag acggcaaacg accgacgacg atcacagtaa atctattagc
     1741 cgacggtcaa ccgatccaac acaaagaagt aagtgaaaaa gatgattggc gctatcgttt
     1801 cacaaatctg ccaaaataca gagacgggca agaaatcatc tatactgtaa cggaagacaa
     1861 cgtgcccgaa tacagtacga cgattgacgg gtataacatc aaaaacagtt atacaccgga
     1921 aaaaacaaat atatctatac caggtagcca tataacaccg tggaaaaatt ttccagagaa
     1981 gacagagaga gagaaaatta gaaatctatt ctctcaaaaa ttgcaaatat ttgctcatac
     2041 aggagaatcc agcgaacaaa gaagcaatta tattagtaag agagctgtac ctaaacagat
     2101 aacaaataaa tcaaaaagca ttcttcctaa aacaagtagt aaaaaatcta gttttgctgt
     2161 aatcattggt ttattgatag taactatctg tgctggga
//
LOCUS       DAIGOK010000204         2139 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.210, whole genome shotgun sequence.
ACCESSION   DAIGOK010000204 DAIGOK010000000
VERSION     DAIGOK010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.210"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..93
                     /locus_tag="NOA01_002628"
     CDS             <1..93
                     /locus_tag="NOA01_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HCI0633774.1"
                     /translation="RGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            72..359
                     /locus_tag="NOA01_002629"
     CDS             72..359
                     /locus_tag="NOA01_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCI0633775.1"
                     /translation="MIGNPSLNHRYRKGFYSLFKTLQQNQNTNSYQAAILTAVNLGGD
                     TDTTAAVTGALAGILYGGEVIPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            428..694
                     /locus_tag="NOA01_002630"
     CDS             428..694
                     /locus_tag="NOA01_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCI0633776.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            684..953
                     /locus_tag="NOA01_002631"
     CDS             684..953
                     /locus_tag="NOA01_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCI0633777.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            complement(1212..1478)
                     /locus_tag="NOA01_002632"
     CDS             complement(1212..1478)
                     /locus_tag="NOA01_002632"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633778.1"
                     /translation="MSRRSLEAHSISQLVGVRNGKNVAQNQKIQLREPGMGSYRFLIR
                     RSWSYASTDSFKSFWPFRVGKNGEVSRLGGGTQPTKVTATGEGT"
     gene            1660..2109
                     /locus_tag="NOA01_002633"
     CDS             1660..2109
                     /locus_tag="NOA01_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCI0633779.1"
                     /translation="MYNEKKIVDIVTLKMVVAEKSLPIEKVRSSFEVGNWLIEEIGDY
                     AQEHPALLCLNTKNEITHFSIVHVGTLNQSVAHPRCIFQRAILANAARIMISHNHPSG
                     NTTPSENDIQFTNRLIKAGEVMGIELLDHIIVGQKKYLSLREEGYWN"
BASE COUNT          699 a          407 c          460 g          573 t
ORIGIN      
        1 cgaggattat ataagaaaac ccgaaaagaa ggcactctct tcgggttttc ggtctgtact
       61 gaaatcaagg tattattggg aatcccagct taaatcatag ataccgtaag ggattttatt
      121 ctttatttaa aactttgcaa cagaaccaaa atacgaatag ttatcaagca gccatattga
      181 ctgcggtcaa tttaggaggg gatactgata ctactgctgc cgtaactggt gctcttgcgg
      241 gtattcttta cgggggtgaa gtgatccctg aaaattggag aaaatcgctt caaaatgacc
      301 agcttcttaa aacgatcagt cagagatttt gcgataccgt gctatctcaa agaatttgaa
      361 taatggatat gatgcaaatc ttgtttgtac taacgttgta ttgacaaaaa ggattttatt
      421 tagtacaatg ttagtacaaa tgatgaacgg aggtagaatc atgtctcaat caaccattac
      481 atttaggatt cccgacgatg aaaaagaact ggtattagaa tatgcaaaag cacacaattc
      541 ttcattgacc gaactttatc gaaatgctgt actggataaa atcgaagatg aaatcgatct
      601 acggacttta cggagtgcaa ttgaagtttc caaagagaaa aaagagagtg gtatttctca
      661 agaggaaatg gagaaactgc taaatgaatt ataaagtcat ctacatgccg caagcccaga
      721 agcaattaaa aaaaatggac agaaaccaag ctaaaataat tgtcgcctgg atcagaaaga
      781 atttagaaaa cacgactgac ccgcgtcagc acggaaaagg tttgactgga aataagagtg
      841 gggaatggcg ctatcgaatt ggaaactatc gaatattggc taatatttgt gattctgaaa
      901 ttgaaatcca gatattctca attggacata gaagtacaat atataaaaga taaccgtttt
      961 tggaacgaag tagagtttcg aaagtcaatg tataatcttt tgaattttgc ataataccac
     1021 aggtagaaac tacccgatac tatcggttgg tttctgcttt tttatttggt cttgaagaag
     1081 aaaacgagtg atctgtggca aagaaagaaa caaaaaaagg acaaaactgt tgcttcgcct
     1141 gcggtgcagt cgcccctggc tcaaagcgca acagtttttt tggctagtgg caaggtctaa
     1201 ctgcttgttg gctacgtgcc ttcgccagtc gcagttacct ttgttggctg cgttcctccg
     1261 ccaagtcggc tgacctcacc atttttgcct acgcgaaaag gccaaaagga tttaaaggaa
     1321 tccgtcgaag catagctcca acttctccgg atcaaaaagc ggtaggaacc catgccgggt
     1381 tcccttagtt gaattttttg gttttgcgct acatttttac cattgcggac gccgactagc
     1441 tgcgaaatag aatgagcctc caggctacgc ctactcattc tacttctcgc aagtggcttc
     1501 gccaggcggc aagggcaagc aatgtgctac gcacccttgc cgcctgactg ctgcatggta
     1561 aaagcttcgc aacaaaacgc aaaaaattca gcctttggct accgctgtgt agcgcaaaga
     1621 ccaaaaggag aaggtcaaaa aatggaacca actacagtta tgtataacga aaagaaaatt
     1681 gttgatattg tcacactcaa aatggtagta gccgaaaaaa gtttgcctat tgaaaaagtg
     1741 agaagttcct ttgaggttgg aaattggttg attgaagaaa ttggtgacta tgcacaagaa
     1801 caccctgcgc tactttgtct gaatacgaaa aacgaaatta cccatttttc aatcgttcat
     1861 gtgggaacat tgaatcagtc agttgctcac ccacggtgca ttttccaacg tgctatattg
     1921 gcaaatgccg ctagaattat gatttcacat aatcaccctt caggaaacac aacacctagt
     1981 gaaaacgata ttcagtttac caatcgatta ataaaagccg gtgaagtgat gggcattgaa
     2041 ctacttgacc atattattgt cggtcaaaag aagtaccttt ccttacgtga ggaaggttat
     2101 tggaactaac gattaagact gcctgataat aaggcagtc
//
LOCUS       DAIGOK010000205         2132 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.211, whole genome shotgun sequence.
ACCESSION   DAIGOK010000205 DAIGOK010000000
VERSION     DAIGOK010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.211"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..76
                     /locus_tag="NOA01_002634"
     CDS             <1..76
                     /locus_tag="NOA01_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCI0633780.1"
                     /translation="VNQICEITNVSRASLYRKLSEVNN"
     gene            290..985
                     /gene="vanR"
                     /locus_tag="NOA01_002635"
     CDS             290..985
                     /gene="vanR"
                     /locus_tag="NOA01_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HCI0633781.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            963..2117
                     /locus_tag="NOA01_002636"
     CDS             963..2117
                     /locus_tag="NOA01_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HCI0633782.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          715 a          397 c          453 g          567 t
ORIGIN      
        1 tgtaaatcaa atttgtgaaa ttactaatgt atctagggct tcattataca ggaaattatc
       61 agaagtgaat aattagccat tctgtattcc gctaatgggc aatattttta aagaagaaaa
      121 ggaaactata aaatattaac agcctcctag cgatgccgaa aagccctttg ataaaaaaag
      181 aatcatcatc ttaagaaatt cttagtcatt tattatgtaa atgcttataa attcggccct
      241 ataatctgat aaattattaa gggcaaactt atgtgaaagg gtgataacta tgagcgataa
      301 aatacttatt gtggatgatg aacatgaaat tgccgatttg gttgaattat acttaaaaaa
      361 cgagaattat acggttttca aatactatac cgccaaagaa gcattggaat gtatagacaa
      421 gtctgagatt gaccttgcca tattggacat catgcttccc ggcacaagcg gccttactat
      481 ctgtcaaaaa ataagggaca agcacaccta tccgattatc atgctgaccg ggaaagatac
      541 agaggtagat aaaattacag ggttaacaat cggcgcggat gattatataa cgaagccctt
      601 tcgcccactg gagttaattg ctcgggtaaa ggcccagttg cgccgataca aaaaattcag
      661 tggagtaaag gagcagaacg aaaatgttat cgtccactcc ggccttgtca ttaatgttaa
      721 cacccatgag tgttatctga acgagaagca gttatccctt actcccaccg agttttcaat
      781 actgcgaatc ctctgtgaaa acaaggggaa tgtggttagc tccgagctgc tatttcatga
      841 gatatggggc gacgaatatt tcagcaagag caacaacacc atcaccgtgc atatccggca
      901 tttgcgcgaa aaaatgaacg acaccattga taatccgaaa tatataaaaa cggtatgggg
      961 ggttggttat aaaattgaaa aataaaaaaa acgactattc caaactagaa cgaaaacttt
     1021 acatgtatat cgttgcaatt gttgtggtag caattgtatt cgtgttgtat attcgttcaa
     1081 tgatccgagg gaaacttggg gattggatct taagtatttt ggaaaacaaa tatgacttaa
     1141 atcacctgga cgcgatgaaa ttatatcaat attccatacg gaacaatata gatatcttta
     1201 tttatgtggc gattgtcatt agtattctta ttctatgtcg cgtcatgctt tcaaaattcg
     1261 caaaatactt tgacgagata aataccggca ttgatgtact tattcagaac gaagataaac
     1321 aaattgagct ttctgcggaa atggatgtta tggaacaaaa gctcaacaca ttaaaacgga
     1381 ctctggaaaa gcgagagcag gatgcaaagc tggccgaaca aagaaaaaat gacgttgtta
     1441 tgtacttggc gcacgatatt aaaacgcccc ttacatccat tatcggttat ttgagcctgc
     1501 ttgacgaggc tccagacatg ccggtagatc aaaaggcaaa gtatgtgcat atcacgttgg
     1561 acaaagcgta tcgactcgaa cagctaatcg acgagttttt tgagattaca cggtataacc
     1621 tacaaacgat aacgctaaca aaaacgcaca tagacctata ctatatgctg gtgcagatga
     1681 ccgatgaatt ttatcctcag ctttccgcac atggaaaaca ggcggttatt cacgcccccg
     1741 aggatctgac cgtgtccggc gaccctgata aactcgcgag agtctttaac aacattttga
     1801 aaaacgccgc tgcatacagt gaggataaca gcatcattga cattaccgcg ggcctctccg
     1861 gggatgtggt gtcaatcgaa ttcaagaaca ctggaagcat cccaaaagat aagctagctg
     1921 ccatatttga aaagttctat aggctggaca atgctcgttc ttccgatacg ggtggcgcgg
     1981 gacttggatt ggcgattgca aaagaaatta ttgttcagca tggagggcag atttacgcgg
     2041 aaagcaatga taactatacg acgtttaggg tagagcttcc agcgatgcca gacttggttg
     2101 ataaaaggag gtcctaagag atgtatataa tt
//
LOCUS       DAIGOK010000206         2084 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.212, whole genome shotgun sequence.
ACCESSION   DAIGOK010000206 DAIGOK010000000
VERSION     DAIGOK010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.212"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(118..708)
                     /locus_tag="NOA01_002637"
     CDS             complement(118..708)
                     /locus_tag="NOA01_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HCI0633783.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
     gene            1231..1392
                     /locus_tag="NOA01_002638"
     CDS             1231..1392
                     /locus_tag="NOA01_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCI0633784.1"
                     /translation="MEPKDKYLDYARVNKDKFIETIIKGKIPDDEKDAVFMAGSPGAG
                     KTEVALGLA"
     gene            1411..1905
                     /locus_tag="NOA01_002639"
     CDS             1411..1905
                     /locus_tag="NOA01_002639"
                     /inference="COORDINATES: protein motif:HMM:NF018154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HCI0633785.1"
                     /translation="MIDADYFRAQFPEYNGKNSSVFQKASSWLVEQSLKYVLEHGYSF
                     ILEDTFAILSAEKNIIRALKNNFRVTIFYVYQDPKVAWDFTRKRELAEGRHVPKEMFI
                     NAFFKSRENIEKVKNRHPEVVLHIMIKDYQNDISEVHYAADNIQLVLPIQYTSKELEE
                     ELHD"
     gene            1898..2059
                     /locus_tag="NOA01_002640"
     CDS             1898..2059
                     /locus_tag="NOA01_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633786.1"
                     /translation="MTEKEQVQQIVKKYNKSIADLSENASAKEFKTVMNYVADEANRK
                     QRKLVGLDK"
BASE COUNT          749 a          330 c          357 g          648 t
ORIGIN      
        1 tatgaatata aatagagtgt atcttctttg ataaatgtca gaaaaagtgt gacaaataac
       61 catcagtaaa attacttgtc atactattga taaggagtat ttaattgaag agaatgatta
      121 ggagaaagga tctccgtact ttttatagta atcatagaat ccaagtttgt atagacgaat
      181 ctttttttct cttatttctt ctgtttcaaa atggactgct tgaagaatca agtctgtgaa
      241 ttctatagga gctgttccat tcgcagtaat caaattacga tctttcacag cttgttgttc
      301 tataaaatct tctttattct gataattttc cgattctaac caaggaaatt gcgcatttcc
      361 ggtgtgtttg taaccttgca ataatccgct ctgagctaaa taatcaactg ccccacaaat
      421 agcagctact ggtacgtctg tttttaacaa tttatcgatt atttgatata aatgatgatt
      481 ttttatgtcc catgaattgc caccgatcat aataaaaagg tcaacttttt caggtatttg
      541 ttcgattaag taatcgattt ttgtttttaa tcctccaatg gaagtgacct ctgattggat
      601 agaagcagtt ttgttttgcc atccctcttt ttggtttagt gtgctagtaa tataagcacc
      661 ttcccaatcg gcatattcct ctaatagaaa cgtaatcgca gttttcattt tcaagctctc
      721 ctttactatc tatgacaagg acaacagttt taacatacag acattcatta aatttggata
      781 gcgacagcga aaacaacaat tcttaagttt tagacagagt atacagacag tcaacttaat
      841 taaatacgaa ctaaataaaa atgacaggta atcatttttg tttaccagca ttataacatt
      901 ttgcatacat ggatgacaaa agaaccaatt cccacttgct tgagtacaat cttaaaagaa
      961 actggaaggc aaaaagtaat tatagaaacg aaaaataaat catatcgggc actcagacat
     1021 gaaaacaacc ctagaaatat ataactagtc caatgaaaaa aaagcgaatg ccgtagaaaa
     1081 caaggattct gcgtacattc atatctcatt ttgcgagcat atctgctatt actgtgacta
     1141 aatgttaata tggttaaaat gaccgttata ttttttatat aaatatcgta taattagtat
     1201 gaatatagaa gaaggaaaga aggtgcaatc ttggaaccta aagacaagta tttagattac
     1261 gctagggtca ataaagataa atttattgaa acaatcatca agggaaaaat ccctgatgat
     1321 gaaaaagatg cggtattcat ggctggaagc ccaggagcag gtaaaacaga ggttgcacta
     1381 ggtttagctt aaaattatga caaccatgtt gtgattgatg cggactattt tagagcacaa
     1441 tttccagaat acaatggaaa gaactcaagt gtttttcaga aagcttcttc ttggttagtt
     1501 gaacaatcat taaaatatgt actggaacat ggctattctt tcatactaga ggatactttt
     1561 gctatcctta gtgcagagaa aaatattatt cgagccctta aaaataactt tcgcgttacg
     1621 attttctatg tttaccaaga tcccaaagtt gcctgggatt tcactcgaaa acgtgaacta
     1681 gctgaaggac gtcatgtgcc aaaagaaatg tttatcaatg cgttttttaa gtccagagag
     1741 aatattgaaa aagtcaaaaa tagacatcct gaagtagtcc ttcacataat gattaaggat
     1801 tatcaaaacg acatttctga agtgcattat gcagcagata atattcaatt agtactacca
     1861 atacaatata cttcaaaaga gttggaggag gaattacatg actgagaaag aacaagtaca
     1921 acaaatcgtg aagaagtata ataaaagtat cgctgattta tcagaaaacg cctcagcgaa
     1981 agaatttaaa acagtaatga attacgtagc agatgaagca aatcgtaagc agcgaaaact
     2041 agttgggttg gataaataaa ttttttaaac catatccttg taaa
//
LOCUS       DAIGOK010000207         2056 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.213, whole genome shotgun sequence.
ACCESSION   DAIGOK010000207 DAIGOK010000000
VERSION     DAIGOK010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.213"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            493..714
                     /locus_tag="NOA01_002641"
     CDS             493..714
                     /locus_tag="NOA01_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633787.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            715..972
                     /locus_tag="NOA01_002642"
     CDS             715..972
                     /locus_tag="NOA01_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633788.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKAAKSAVPTGFLTKQPYIF"
     gene            complement(1108..1710)
                     /locus_tag="NOA01_002643"
     CDS             complement(1108..1710)
                     /locus_tag="NOA01_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCI0633789.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKIRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYA
                     MSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
     gene            complement(1777..1932)
                     /locus_tag="NOA01_002644"
     CDS             complement(1777..1932)
                     /locus_tag="NOA01_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCI0633790.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
BASE COUNT          678 a          464 c          313 g          601 t
ORIGIN      
        1 aaaaaaaaac gctgaaaccc ttgggagagt aaggctaaac agctatcgac caatgaccaa
       61 tgaccaataa ggggggtgtc gtagtccccc cctcctccgc ccaattcata aacaccaaaa
      121 ccaaaaaaca aacctcaggg ctccgccccg aaccccgacc tccttctacc gaaatatatg
      181 caggcgccac gcttactatt tcgttcagct atcggctttt gcaatctaac acgaccgttt
      241 cgctaccagt cgccttacag gctccattct cgcttctctt acgtgtttta acgattgcgc
      301 cgataactaa acgaaataga cgctaaatcg cttaaaacgc aaaatagacc ctttttagag
      361 cattgatttt ttctactagt catttcgata atataaaatt gaccacaaca cagaaaactt
      421 atattataag cttatgtcgt aagtccgacg tactcacgta atatagacgg acgttaaatg
      481 gaaggagcat aaatgagagt gataaaatca gaaaaattca ctttggccga aattatgacg
      541 accagcgaac ttgcacaaga acttgattac aaccaagctt acgttttgcg cttagcgaaa
      601 gaacatctga ctgaaggaaa agaatatcgc tctgccggta gaagaaacta tcttttcagc
      661 ccagaagccc ttaaaaaact agaaaaagtc ataaccacca aagaaggtga gtaaatgcct
      721 aaagaaacaa ctatcaacgt tgaacaacta gaaaaattgc tagaatcata ttccaatgat
      781 tttatcgaat tgaccggcgg aaaagtcgaa gaaaccaccg acctctttga cctacttctc
      841 acaaatattc tggaattaaa ctctgaagaa caaaaagagc ataagggaaa actgatagca
      901 ccttcctaca ggaaggcggc taagtcggct gtgccaactg gttttcttac aaaacaacct
      961 tatatttttt gatatcacaa aaaaatccat tttggaccta ttttcggtca taatatagta
     1021 cctacttttg gtcatagttt cgtccgtagt tttggtcata gtgactaaga ctttcacgca
     1081 caaaaaaaga gctgaaatta acagctctta tcctttattt atttttgaat ttacacttcc
     1141 accatgacga ttcgatttta tatattgccc gataatataa gctgaattat cacaaagaag
     1201 cggaaaccaa tcatcaaatc tttctgacat tgcatagtca attaaatctt tgatttcagt
     1261 gatattctta gaatcaacaa acgacatcat ctctctaatc aactcatatc gttctgcact
     1321 cgttactgac aaataactag caatttctgc accaccatga gcgataatat cacttttagc
     1381 atattgaaat ttttctggat tatccatatg agcaaaatat cgcaccatac cttttgcatt
     1441 agcacatttt tgcgggtttg gagaccgaat cttttgagtt atctcacgta tttgatcata
     1501 cgtcttttta ccgctaaaca ttatcaaaac atgccaatga ggctttttca attcaccgtc
     1561 aggattcaca tctttatcat gaagtggact ctcaacccaa ggaacatgta actcatctaa
     1621 aaactccctc caattttcag gagccgattc aggatagact acaaacgtcc aattccttgt
     1681 gcgttcatct ttagtttttc tactcgtcat gctataatca ccttaattga acaacagtct
     1741 aacagcaaaa gcagtgcgcc aacactaatg cttttgctat tttttttgct cattttcctt
     1801 tccgttagca ttaacccaaa ttgtaataag accagactta ctaagaccca tttgtttcgc
     1861 aattttatca agattatctg ccacttcctt agtaagcgtt acaacgactc tctttttttc
     1921 attactgatc ataattcgta tcctccaacg actttttctt aaaaaccata gtaacaaaaa
     1981 agtaactaag gtgcaacagt ttagatacta attagcgaat aataggcctt aaacccttgg
     2041 cattactgat tttgtc
//
LOCUS       DAIGOK010000208         2025 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.214, whole genome shotgun sequence.
ACCESSION   DAIGOK010000208 DAIGOK010000000
VERSION     DAIGOK010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.214"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            318..587
                     /gene="rpsN"
                     /locus_tag="NOA01_002645"
     CDS             318..587
                     /gene="rpsN"
                     /locus_tag="NOA01_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCI0633791.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            602..781
                     /gene="rpmF"
                     /locus_tag="NOA01_002646"
     CDS             602..781
                     /gene="rpmF"
                     /locus_tag="NOA01_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCI0633792.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            813..1190
                     /locus_tag="NOA01_002647"
     CDS             813..1190
                     /locus_tag="NOA01_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633793.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            1211..1360
                     /gene="rpmG"
                     /locus_tag="NOA01_002648"
     CDS             1211..1360
                     /gene="rpmG"
                     /locus_tag="NOA01_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCI0633794.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            1384..>2025
                     /locus_tag="NOA01_002649"
     CDS             1384..>2025
                     /locus_tag="NOA01_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCI0633795.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDS"
BASE COUNT          738 a          294 c          400 g          593 t
ORIGIN      
        1 ttgaataaaa agttatagtt tctttcatat tgccgtttta agtagaggaa aagctactta
       61 aaacggtatt tgtgtttgat tggactaata aaagtgaatt cttatttttc agagcctaaa
      121 ctgcatattc ttttaaatga tttgattatc tctatgtacc gataaattcg ttttcaaata
      181 gttatctatt catatgagtt ttatttcatg ttatttattg ttgacaagag caactcttgt
      241 tggtaaagta tcagtgtcaa aacgtaacga ttacgatttg aggtgtctaa atcaaaataa
      301 aaggagtata aaggtatatg gcaaaaaagt cgaaaattgc taaagcaaag aaacaaatgg
      361 cgatgattga aaagtatgca gataaacgtc aggaattaaa agccgcagga gatcgcaccg
      421 ctctcgctaa attgccaaga gattcaaatc cgaatcgtct gaggcttcgt gatcagacgg
      481 atggtcgtcc aagagggtat atgcgaaagt tcggtatgtc ccgtattaag tttagagaac
      541 ttgcccatca agggttgatt cctggagtaa aaaaagcaag ttggtaaaag gaggaaatat
      601 catggcagta ccagcaagaa aaacatctaa ggcgaagaaa cggttaagaa gaactcatca
      661 aactgtagta aaaccagaaa tttcatttga cgaagcgaat ggtgattata gacgtagcca
      721 tcatgtatct ttaaaagggt actataatgg aaagaaagta attaaaggag cttcaaaata
      781 gtttaatata taacttttag gagggaatca aaatggaaat tatttaccca cctttagttg
      841 aagaaggatt aaagtattat cttgaaacga cccagcaatc gctagataaa tgcacttttt
      901 atcgttcgat ggtagagaga ggaattatta ctgaaacggg tttaccgacc caacaggcaa
      961 ttgaaaatgg attagtcaaa gattactatg aggaccaagg cttatcattt gatgattttt
     1021 taagaatcta tccaattttt gaagagtatg acgaggagtt atttcaatgt atcgatggtt
     1081 attgggagat acctgttgat atgaaagaaa atttagtttc acaattggaa tctggcgagt
     1141 taacttttga agatgcacaa caaattcaag catatcttga agatcgataa gtattgaagg
     1201 aggaagattt atgcgtcaaa atattatttt ggaatgtgtc gaaactggag aacgtctgta
     1261 tctcaccagc aaaaataaac ggaataaccc tgaacggtta gaattaaaaa agtattcgcc
     1321 aaaattgcgc agacgggcaa tttttaaaga agtgaaatag ctaagtggta aaaaggtgac
     1381 aaaatgggca taccagttac aatcgtttct gggtttttag gggctggaaa aacaacgcta
     1441 atcaatcaag ccttgcaagt ctcaccctac cccagggaag aaattattat aattgaaaat
     1501 gagtttggtg aagttgggat tgatcacaaa ttattagttc atagcgagga acgggtgtta
     1561 caactaaata atggatgtat gtgttgcagt cttcgcagtg atttattagc aatattgatt
     1621 tctttactgg aaacaataga agaacatcaa caaccaattt cacaaatcat cattgaaact
     1681 acaggtatag cagatcccca accaatcatt caaacacttt taacagcgcc tcaactaaga
     1741 ggaagagtat atatcgatag tttattgact gttattgata gtgacagttt tgaacttatt
     1801 gaaagagaac cacaagccct taagcaactt gtcatggcag atcgcatttt tgtgtctaga
     1861 aaacaaaact tgaatttatc tgcactaaaa aaattacaga gagaaatcaa aagtattaac
     1921 cctttatccg atattcgaat gttcacacat agtgaaacga ttcaaaaaaa ggatttttat
     1981 aatttgaaaa aatttaatca tcccttagtt tttgaaagtg attca
//
LOCUS       DAIGOK010000209         1979 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.215, whole genome shotgun sequence.
ACCESSION   DAIGOK010000209 DAIGOK010000000
VERSION     DAIGOK010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1979)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1979)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1979
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.215"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(325..528)
                     /locus_tag="NOA01_002650"
     CDS             complement(325..528)
                     /locus_tag="NOA01_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633796.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSVKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            complement(728..1744)
                     /locus_tag="NOA01_002651"
     CDS             complement(728..1744)
                     /locus_tag="NOA01_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HCI0633797.1"
                     /translation="MANENQLSEIQSKIEGRGAEKRTTINQLEKLNRISNRRLSVQGT
                     TTPLKLNWSIDRITIVGKLNDTIAYWTKNGVVFVDFEQLMRLNENQGYLKSVGINGWQ
                     LFDQYGEDIAYIEILKFHEGKGRIDFNPNKIGQFLASSMKGFIHDLFLDPHFSRADIA
                     CDIVDVPDDFITQYRIVDPISFKPIYGRSGKLETAYWGSRASERQVRLYNKKLEQERK
                     KVIVPKEIDTWWRLEMQLRRGKATDWHAMVQESLDSFASPHFLPIDIKPIDKIVIDGL
                     IAEPSNWSIIARHTKYKYRNLLKQESQNDELTNHLRETFKESADELKKELDTWLLGLD
                     VTEK"
BASE COUNT          559 a          459 c          308 g          653 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggttttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cacgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaaca ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaatgct ctccgagggt attcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt cacgcaaaat aatcgcttat
      661 cgtaaatcca cgtcactaaa cattgacgta aatttactct cactcatctg acttttctta
      721 ttcggtatca cttctctgtc acatccaacc ccaaaagcca cgtatccaat tcttttttta
      781 attcgtctgc agattcctta aaagtctctc gtaaatgatt cgttaactca tcattttgac
      841 tttcttgctt cagcaaattc cgatatttat attttgtatg tctagcaatt atcgaccaat
      901 tagaaggctc agctatcaaa ccatcaataa ctattttatc aataggtttt atatcaatcg
      961 gcaaaaaatg cgggcttgca aaactatcta agctttcctg aaccatcgca tgccaatccg
     1021 ttgctttgcc acgtcttagc tgcatttcta accgccacca cgtatcgatt tcttttggca
     1081 cgattacttt ttttcgttcc tgctctagct ttttgttgta cagtcgcact tggcgctcac
     1141 tggcacgact gccccagtac gctgtttcta gcttgccaga acggccgtag attggcttaa
     1201 aagaaatcgg gtctacaata cgatactgag taataaagtc atccggcaca tctacaatat
     1261 cgcaggcaat atccgcccgg gaaaaatgcg gatctaaaaa taggtcatga atgaatcctt
     1321 tcatggaact agctaaaaac tggcctatct tattcggatt aaaatcaata cgaccttttc
     1381 cttcatgaaa tttaagtatt tcgatatacg caatatcttc tccatattga tcgaataatt
     1441 gccagccgtt tatcccaact gatttcaaat atccctgatt ttcgttcaaa cgcattaatt
     1501 gctcgaaatc gacaaaaaca actccatttt tcgtccaata agctatggta tcattcagtt
     1561 ttccgacaat tgtaattcga tcaatcgacc aatttaattt taggggggtg gtcgtacctt
     1621 gcacacttaa ccgcctgtta gaaatacggt taagtttttc gagctgattt atcgttgttc
     1681 ttttttctgc ccctcgacct tcaatttttg attgaatttc actcaactga ttttcatttg
     1741 ccattttcgt cctccaaacg aaaaaagaga cttttggaaa gaatttccag agttcacgta
     1801 accctaaaaa ttcatatcca aaaatctcta gcttgaagaa acctaaaaat attcgtataa
     1861 tgaacctgag ttacggagct aggtgaagta gtttccgtaa atagtcgaga gtgttagcgc
     1921 actgctcggc ttttattttg ctcttttttc tcgttttctt tgattatagc actcctcct
//
LOCUS       DAIGOK010000210         1908 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.216, whole genome shotgun sequence.
ACCESSION   DAIGOK010000210 DAIGOK010000000
VERSION     DAIGOK010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.216"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            100..1824
                     /locus_tag="NOA01_002652"
     CDS             100..1824
                     /locus_tag="NOA01_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCI0633798.1"
                     /translation="MKKKLLTLFLVMSFSIVLSVYYNTIVFSEQINYTEEQYNIEKEK
                     ALKVGYSEEQFKQIMEIPTNLSNENSETRIVNYTMTSNQTKVINKAMEQIGKPYEWGA
                     SGPNSFDCGGLVKYVYKQAVNIELPMGTTNQEQYGTEVSLNSLKPGDLLFYGNRGATY
                     HVGIYKGNGVMIHAPQPGETVKEVNIQYFYPNFAKRILPDEPDYPYIDYNKMVTVTKA
                     WSIWNDLQFSHEIKKAIIGDNYKIGKVYTNPENNNKYGEILVSNKVYGYINFYAVKEL
                     TSVQINRYLTSKDSGQPTWGNLECTISKGQTTKDKIYFVKGAYNLGDGKYLYSIYKDQ
                     DSSEWLGYLKAHVSLAYTPIEEINKNVTVTKNWSIWNNLQWEKEIEKPQIGSVFSARL
                     KLTNVSNNAVYYKLYKSGKFYGYINAEAVKDLTTTKLNKYVTFSVNNEDFWSSLDVNY
                     SKGKTERGRVFYISISYNTADNQPIYSVYTDETCTEWRGYYKGNNFEDTQITMLENKS
                     VKVTKSGYTVWGDLNFWTKSGISNTGDIYTTDRKFYNFTNNAYYYELKKDGRVYGYIN
                     SEAAVEMN"
BASE COUNT          750 a          235 c          341 g          582 t
ORIGIN      
        1 attacaatat ttcaacaaat taaaaaaaat tatatcttct taaacaatga taatattaaa
       61 atgcaagcgc ttgtaaaaaa taaggagaag aggtaaaaaa tgaagaaaaa actattaact
      121 ttatttcttg tgatgtcttt ctcaatcgtt ttgagtgtgt actacaatac tattgttttt
      181 tcagaacaaa taaactacac agaagaacag tataatatag aaaaagaaaa agctttaaaa
      241 gttggttata gtgaagaaca gtttaagcag attatggaaa tacctacaaa tttatcaaat
      301 gaaaatagtg aaacaagaat agtaaattat actatgactt ctaatcaaac caaagtcatt
      361 aataaagcaa tggagcaaat tggaaagcca tacgaatggg gagcatcggg accaaattct
      421 tttgactgtg gtggtttagt gaaatatgtt tacaaacaag ctgtaaatat tgaactgcct
      481 atgggaacca ccaatcaaga gcaatacggt acagaggtaa gtttaaattc acttaagcct
      541 ggtgatttat tattttacgg caatcgaggt gcaacttatc atgtaggtat atataaaggt
      601 aacggtgtaa tgattcatgc accgcaacct ggagaaactg taaaagaggt taacatacaa
      661 tatttttatc ctaactttgc aaaaagaatt ttgcctgatg aacctgacta cccttatatt
      721 gactataata aaatggtaac tgttacaaaa gcttggtcaa tatggaatga tttacaattt
      781 agtcatgaaa ttaagaaagc tattattgga gataattaca agatagggaa ggtttatact
      841 aatccagaaa ataataataa atatggagaa atattagtaa gtaataaagt atatggatac
      901 attaattttt atgctgttaa agagttgacc tctgttcaaa taaacagata tttaacttct
      961 aaagatagtg gtcaaccaac ttggggaaat ctagagtgta ctataagtaa aggacaaact
     1021 acaaaagata aaatatattt tgttaaagga gcatataatt taggggatgg aaagtatcta
     1081 tattctattt ataaagatca ggattcctct gaatggttag ggtatttaaa agcacatgtt
     1141 agtttagcct atactcctat tgaagaaatt aataaaaacg taactgttac taaaaattgg
     1201 tcgatttgga ataatctaca atgggaaaag gaaattgaaa agcctcagat tggttctgta
     1261 ttttcagcta gattaaagtt aaccaatgta tcgaataatg ctgtctatta caagctttat
     1321 aaatctggaa aattttatgg ttatatcaat gctgaggctg tcaaagacct tacaacaaca
     1381 aagcttaata agtatgttac atttagtgtt aataatgaag atttttggtc ttctttagat
     1441 gtaaattatt caaaaggaaa aactgaaaga ggccgagtat tttacattag tatttcatat
     1501 aataccgcag ataaccaacc gatttattca gtatatacag atgaaacatg tacggaatgg
     1561 cgagggtatt ataagggaaa caactttgaa gacactcaaa ttactatgct tgagaataaa
     1621 agcgtgaaag taacaaaaag tgggtataca gtatggggag acttgaattt ttggactaag
     1681 tcaggaatat cgaacacagg agatatatac accactgacc gaaaatttta taattttacc
     1741 aacaatgctt actattatga attgaaaaaa gatgggaggg tctatggcta tataaatagt
     1801 gaagcagctg tagagatgaa ctaaaatatt gctgtccctt aatcttatgg ggcagcaata
     1861 tttttatatt attaatcatt tttctgaagc gagggtagaa gaattaat
//
LOCUS       DAIGOK010000211         1859 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.217, whole genome shotgun sequence.
ACCESSION   DAIGOK010000211 DAIGOK010000000
VERSION     DAIGOK010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.217"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(<1..614)
                     /locus_tag="NOA01_002653"
     CDS             complement(<1..614)
                     /locus_tag="NOA01_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633799.1"
                     /translation="MTETKFIQDPWSKMTTRNGLRGDEAISALQKSIRRGKEKEACEF
                     AYEMYITSPQFEEKLWRRLLAISVEDVGMGNPMAAIMINNLNAMRKEFQYNESDRAMY
                     FFHAIRYLCQSEKDRSSDLLKNIVIKSFAMGYIPEIPDIALDKHTVRGQAMGRGSEHF
                     LHEASKVIPQAKVDNDYKEQYEKIIETYDPDHVVPSTFSFNPWQV"
     gene            655..1347
                     /locus_tag="NOA01_002654"
     CDS             655..1347
                     /locus_tag="NOA01_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCI0633800.1"
                     /translation="MDVEMLIDKYQLNDSEAEVLRFMKKNRQSLKNIGIRDVAKASYV
                     STATIINMAKKIGYSGYSELVFALGNSQSDITMPSTFSNEEKEAFIHLMSAYRDKRIM
                     ILGSGFSQNLANYFSESLNLYGFRATANSHLEFLRQQVQQDILLFIISNSGDTVRLAE
                     LAKIANDHFIDVIAFVGQKNSKIGTLATLTISTETYIPQKISDYQPNLFFGTTLNQFE
                     LLLSAALKAIYK"
BASE COUNT          571 a          348 c          331 g          609 t
ORIGIN      
        1 acttgccaag gattaaatga aaacgtactt ggcacgacgt gatctggatc ataggtctcg
       61 atgatttttt cgtattgttc cttgtagtca ttatcaactt ttgcttgggg gatcacttta
      121 cttgcttcat gcaaaaagtg ttctgaacca cgccccattg cctgtccccg aacagtatgt
      181 ttatccaacg caatatcagg aatttctgga atatatccca tagcgaaact cttgataaca
      241 atgttcttaa gtaaatccga tgaacgatct ttttcagatt ggcataggta acgaatcgcg
      301 tggaaaaaat acatcgcacg atcggattca ttgtattgga attctttgcg catggcgttt
      361 aagttattga tcatgatggc ggccatagga ttgcccatgc ccacatcctc aacactgatc
      421 gccaatagcc ttctccagag tttctcttca aattgtggtg aggtgatata catttcataa
      481 gcaaattcac atgcttcttt ttcttttcct cgacgaatcg atttctgtaa tgctgagata
      541 gcttcatctc cacgtaagcc gttacgggta gtcatttttg accacggatc ttgaataaat
      601 ttcgtttctg tcataatata atactcctat tagataaaat ttgaggtgaa tcttatggat
      661 gttgaaatgc ttatagataa ataccaactg aatgactcag aagctgaagt cttacgattt
      721 atgaaaaaaa atcgtcagtc tttaaaaaat attgggattc gtgacgttgc caaagcatcg
      781 tatgtttcta ctgcaacgat tatcaatatg gcgaagaaaa ttggatattc cggttatagt
      841 gagttggtat ttgctctagg aaacagccaa tctgacatta cgatgccaag cacattctcc
      901 aatgaggaga aggaagcatt catacattta atgtctgcgt atcgcgataa acggattatg
      961 attcttggct caggtttttc gcaaaatcta gccaattatt tttcggaatc tcttaatcta
     1021 tatggatttc gtgcgacggc caatagtcat ttagaatttc ttagacagca agtgcaacaa
     1081 gatattctgc tttttattat ttccaattct ggtgatacag ttcgattagc cgaattagct
     1141 aaaattgcga acgatcactt tatcgatgtg atcgcatttg ttgggcaaaa aaattcaaaa
     1201 atcggtacgc tggcaacatt aaccattagc acggaaacct atattccaca aaaaatatct
     1261 gattaccaac caaatctttt ctttggcacg acactgaatc aatttgagct tttgttgagc
     1321 gcggcgctga aggctattta taaatagaag aacgctgtgg ccactgtttc ttcaaaatct
     1381 agtataaaga atcttcttta gaatgtaatt gttttctgtg tgttctgaac atgtttagga
     1441 atttccaaac ttgtttagaa aactttctca ttccctccgc tgtagaattg aactcacata
     1501 aaaaacatct aaaccaaaat atctacaagc ttggaaagga taataggttg ttattaaaaa
     1561 taatttttat tcctgaacct gaataattca tagctttaat tctttggcac attttattga
     1621 aatatgtatc actaatatcc ctatcaactg attttgacta aaaagttact tcaaacattc
     1681 actgttcaaa aatattgagg aataaatttt gggtgtaagg cattttgagc atacttctcc
     1741 ctggataact tcccagtaaa aaaatcgtta gattgttgga ttagatccat tgactgcctc
     1801 gcctcaggcg agcaaagacc ctgtagaatt cattctgata cacatgataa ctagataat
//
LOCUS       DAIGOK010000212         1828 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.218, whole genome shotgun sequence.
ACCESSION   DAIGOK010000212 DAIGOK010000000
VERSION     DAIGOK010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.218"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(111..1280)
                     /locus_tag="NOA01_002655"
     CDS             complement(111..1280)
                     /locus_tag="NOA01_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCI0633801.1"
                     /translation="MNDFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            1468..1767
                     /locus_tag="NOA01_002656"
                     /pseudo
     CDS             1468..1767
                     /locus_tag="NOA01_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292163.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
BASE COUNT          598 a          301 c          276 g          653 t
ORIGIN      
        1 aaaaataacc acccgaaggt ggctgaagaa taagctttta tttgtaagtt tccagaatag
       61 gttgcgtcaa gaattatgtg taaatggaaa agcccattct gttgatcaag ttaaaacatt
      121 gattctaatg tatcatgtat ttctttaaaa cctttatgaa ctcggcatag aaacttttga
      181 ttatagttgt tgaattgaga tactagaaat ctctccagag attcttcatt aggaaattgc
      241 tcttttcttt tggtatattt ctttaattgt ttgttaaaac cttcaatcaa attagttgaa
      301 taaatagttc ttctgattga cggagggaaa ttataaaagg ttaatatagc aggattcatg
      361 agcaatttaa ccactcgtgg atactgcttt ttccatttat ctatcataaa ccttatttga
      421 ttatttgctt cttcttttga acttgcttga taaactaatt tgaaatcgct acagatttct
      481 tgtcgatcac taacacgaac cttatgagcg atatttctag atatatggac acaacattgt
      541 tgaaattgag catttggata aacactatga atactatcgt taatgccact taaaccatca
      601 gttacgacta ataaaacctc ttttaaacca cggtctttta ggtcttgaag aatctctttc
      661 caaacataag cagattcagt tggagcaaga ctaaacccaa gaacctcttt ggttccatct
      721 aatcgaatgc caattacaat ataaactgct tcttttgaaa cagtttgtct ttttaaagga
      781 atgtgagtag catccataaa aataacagag tatctggatt ctaaggacct ttctttaaag
      841 gcaataatat cttcagaaac tattttagtc atatttgaaa tggtttgtgg cgtatagtga
      901 tgcccgtaca ttttttcaat caattcagag atttcagaca tagtaattcc tttttgaaat
      961 agctgaataa tagtggtttc taaagagtca ttggttcttt tataagcagg taaagtttgt
     1021 tgagaaaact taccatttct atctctagga atcaccaaat ttaattctcc atactctgtt
     1081 ctgaatgaac gtgaataatt cccattacgg gaattacctg aattgaaccc agcacgatca
     1141 tatttttcat aatcaagaaa agctgttaat tcagcctgca ataatgaatt aacagctaat
     1201 tctaaatgac atcgaaataa ttcatctaaa tcacctttat tgattagtgt ttccataatt
     1261 tctgtagtaa aatcgttcat atggaagtcc tcttttctgt gaatgtgttg tggtaactct
     1321 attctacaga agggacttcc ttttttctat ttacacaaaa tattttacac tctcatcaaa
     1381 tcattagaat cgtgataaga cattgtatta aatctaatcc tttatccatt ttttatcaat
     1441 cttacttcgc accatctcca atacttatta ttagcctctc ttggagcatg taaagttatt
     1501 gtagctcggt ctttcgctaa agtgcagggc ataaatttaa acgatatccg tgtagagata
     1561 gatggagtat tagatcctga tggttttata ggaaaaaata aagcagctaa aataggctat
     1621 tctgcaatcg tatctaaatt ttatattgaa gctgataata ctgatgaaga gatttcagat
     1681 tttatcgcat ttattgatcg tacttgtccg gtagctgata ccattaaaaa tgcacctacg
     1741 actaaatcag aaatccataa attataaact atgaattaaa aaagattctt ctcattttgc
     1801 actaaacagg tatttctatt ttatttct
//
LOCUS       DAIGOK010000213         1815 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.219, whole genome shotgun sequence.
ACCESSION   DAIGOK010000213 DAIGOK010000000
VERSION     DAIGOK010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.219"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(69..1109)
                     /locus_tag="NOA01_002657"
     CDS             complement(69..1109)
                     /locus_tag="NOA01_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCI0633802.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
BASE COUNT          622 a          277 c          224 g          692 t
ORIGIN      
        1 tagaataata aaaaattttt aattttaatg ttactccatg aagcaacaat cactttgtca
       61 gtaaagtttc actctaacca gtctttcgta atagtgcttg gggagcttgc ataattcaaa
      121 cgtttttcac ttgatacttt agcagctatt ggtttttcga taaaccaatt ttttacatag
      181 acaaaaatca ggttctggat attttcgact ttttcggttt ttaatttttg ataggctttt
      241 aacagtgtcg tgtataattc ttgttctgca ttgacataat aactatcgag ttcttcaaaa
      301 actatcgaaa tatcactttc tatttcagct tttttctttg cattatgaat agtctggacg
      361 gttttttgag cttcactgta acttgaagaa aatgttttta tcaaatttaa tactcgttca
      421 gggacaaacg tagcaatgct gtcgtttgtc ataattgtct caaaattgtc taacaacaat
      481 tgatcttgta ggcggtcttt ttcagtgtct atattgtgtc tattagtgtc taatatatta
      541 ttattaagtt ctggtatgat tttcataccc tctgatttat caacgttttg gcacggtatg
      601 attttcatac cctcttgagc cccaagggat tcagcgtctg atttttcgtc gagggtgggt
      661 atgattttca taccctctga tttatcaacg ttttccctat tctctctatc aaatttttcg
      721 agtaagtaga tatcattttc tgagactttt aattctgcta gatatagtct attaggatta
      781 tttttcccta actgtttgtt aaatcccatc tgcttttgta ataataaacc aaaggattcc
      841 aactcttttt tgattgctat cgctttttgt ttttggcagt ttaatactct acatagttca
      901 tttgtcgtat aagtaaaata aacattacct tcttcatcga tttgccgttt gctgattgcg
      961 atctctaatc ttgcttttaa aagcatatag gcaatcttag ttgaatcttt catttttttg
     1021 tattcgtcag aaacaataaa gacttttgga aattgataaa ataattcgtt gtaaactcta
     1081 tccgccttaa aaaatttaaa atcagtcatt gtagatcctc tactttcacg ttgttttatt
     1141 tatgtaaaat tacctactct tttcgaaaat agcaaagata aataaaaaat gcaaacgcat
     1201 taaaactgtt ttttaattaa atactaccta aaatcagaaa taaaatgaat tatgtactac
     1261 ctttaaagtc attaagaact actttaaata aatattattt ataattaaat actaccagaa
     1321 aaacgtatga ataaaattta gttttaaaat taaagataat taagtactac cagtgtcaaa
     1381 acaaaaaata aattaagaac taccttatgc atataaaaca tactaccatt actagtaata
     1441 tatactacct tatttcagta gtatatatta tattattctt ataagacaag gctttaggct
     1501 agaaaacaat agattatata ctactaaaaa atacaataag tactaccaat gtcagatata
     1561 tataataatt atgtactacc attaaaatta acttatttaa taagatacta ctgtgttaat
     1621 ctgaaaaaaa ttactttaaa gtagttacat actacctgtt aatttagata ctaccattaa
     1681 tacttaaatt agatactacc tatatttata ttaagtacta ccgttctaag aataagataa
     1741 taaaaaaata ctaccaaatt gcaattaaat actacttaaa atgtaaaaaa taagaactac
     1801 ccaaattagg tagtt
//
LOCUS       DAIGOK010000214         1797 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.220, whole genome shotgun sequence.
ACCESSION   DAIGOK010000214 DAIGOK010000000
VERSION     DAIGOK010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.220"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..449
                     /locus_tag="NOA01_002658"
     CDS             <1..449
                     /locus_tag="NOA01_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCI0633803.1"
                     /translation="NLCVELNNEVSQSTDQTPDERWQTNEKEHLHPYQKNLLNPYFED
                     FIRRKVTKEEMVIFRKCRYSVAPRYIGKEVEIELSDKEDQVQIYYNGELIRSHPLTTK
                     QLNYHEEDYFQILKSDVMSHKEDDGIRAYTRESLKQYDLVEVADEE"
     gene            439..1179
                     /gene="istB"
                     /locus_tag="NOA01_002659"
     CDS             439..1179
                     /gene="istB"
                     /locus_tag="NOA01_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HCI0633804.1"
                     /translation="MKNSHYQELMTNLETLGLNHMREYVPNYIEVANREELSLTEALL
                     ELTRREVDHQNKQKMNRVVARARFSKRTSLEEFDFAFQPSINKREVLDLRHMGFVEKH
                     ENLVFIGSPGVGKTHLTIALGIEACRQGYRTLFITCHELLLRLRTAYEKGTLERVLKR
                     YARYDVLIIDEIGYLPIQKQEADLFFQLLAMRYEHRSTIITTNIVLSRWGELFQSSEI
                     AAAILDRLVHHVKVFKITGKSYRMRDKI"
     gene            1589..1753
                     /locus_tag="NOA01_002660"
                     /pseudo
     CDS             1589..1753
                     /locus_tag="NOA01_002660"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          580 a          369 c          351 g          497 t
ORIGIN      
        1 ataacctttg tgttgagtta aataacgagg tttctcaatc aactgatcaa acaccagatg
       61 aacgttggca gacgaacgaa aaagagcacc tgcatccata ccaaaaaaac cttttgaatc
      121 cctattttga agactttatt cgccgtaaag tcaccaagga agaaatggtc attttccgta
      181 aatgcaggta ctctgtggcg cctcgctata tcggtaaaga agtcgaaatc gaactctccg
      241 ataaagaaga ccaagtacaa atctattata acggagaact gattcggtcg catccattaa
      301 cgacaaaaca gctgaactac cacgaggaag actatttcca aatcttgaaa tccgatgtca
      361 tgagtcacaa agaagacgat ggaatcagag cgtatactcg tgaaagtctc aagcaatatg
      421 atctggtgga ggttgccgat gaagaatagc cattaccaag aactgatgac caacttagag
      481 actcttggtt tgaatcatat gcgggaatat gttcctaact acatcgaagt ggccaatcga
      541 gaggagcttt ctttaacgga agctcttctc gagctgacac gcagagaagt tgatcatcaa
      601 aataagcaaa aaatgaatcg tgtggtggca cgcgcccgct tttccaagag aaccagcctg
      661 gaagagtttg attttgcctt ccagccaagt atcaataagc gcgaagtgct agacttaagg
      721 cacatgggct ttgtggaaaa acatgagaac ttggttttta tcggcagtcc tggtgtaggc
      781 aagacacatc tgacgattgc attgggaatc gaagcctgtc gtcaaggcta tcggacacta
      841 ttcatcacct gtcatgagct gcttttgcgt ctgcgaacag cctatgaaaa aggtacccta
      901 gagcgtgttc tcaaacgata cgctcgctat gacgtcttga ttattgatga gattggctat
      961 cttccgattc aaaaacagga agctgattta ttctttcagc ttcttgccat gcgctatgaa
     1021 caccgctcta cgatcattac gacgaacatt gtcttatctc gttgggggga gttgttccaa
     1081 agttctgaga tcgcagctgc catccttgac cgtttagtac atcacgtcaa ggtgttcaag
     1141 atcacaggca agtcttatcg tatgagagac aagatctaaa gtgtcgattg tcgacaatta
     1201 ctattgccga aaacctacat ttttatcctg cccaaaacct acaattttaa gctgcccttg
     1261 acatcaaaaa gacacttcac aagctaagtg ctatcgacaa ttgaaacctt tgtataaatt
     1321 acttctataa tcagatgacg agttggatta tacaacattc aagaaatggc agaattttca
     1381 atggcgctat ttaacagaaa gtgaagtagt tgattgtcta ccctcaatct cttatgaact
     1441 taaaatggct tagcagtatt atcaaaagct gcttaacaat tatcacaaga aacagctagc
     1501 tacttcgaga aatgaccaaa gaagttccct cgaaacttca ctatcttaaa aagccttttt
     1561 catatctgaa aaaggctttt aactagcatt ttcgaaaaag tattttaata caaaattaga
     1621 gaatcttcgc acacatatca aaacattgaa acgatttgct tatggatttt gcagttttac
     1681 caatatgcgc ttgtggattt ttctaatcaa cgggttaata agctatgcat taaaagctag
     1741 taaatcttac taactttgtt gatcataaat gaccttatca gcccgatttg acaaaga
//
LOCUS       DAIGOK010000215         1796 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.221, whole genome shotgun sequence.
ACCESSION   DAIGOK010000215 DAIGOK010000000
VERSION     DAIGOK010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.221"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            207..404
                     /locus_tag="NOA01_002661"
     CDS             207..404
                     /locus_tag="NOA01_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633805.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            510..992
                     /locus_tag="NOA01_002662"
     CDS             510..992
                     /locus_tag="NOA01_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633806.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(1050..1646)
                     /locus_tag="NOA01_002663"
     CDS             complement(1050..1646)
                     /locus_tag="NOA01_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCI0633807.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
BASE COUNT          565 a          271 c          358 g          602 t
ORIGIN      
        1 ataattagtt tttaaggaat atgttttaat tttttgaaaa aaattcttct aaatacaaca
       61 gctgttttaa ttgtgaaaac gaaaacagta cgctataatg cctgtaaggt tgatttttaa
      121 ctgtaaataa aaatcaatga gttaaatctc caaagaaata gcagtggtaa tgtggagaaa
      181 agttccagca gtttaggagg attttgatga cggaagaaat gattaattta ggagaacaat
      241 atgcatgcaa accaattggt tttacaaaaa cggtcattgg tgaagttgta tcaaaaatga
      301 caaactgcgc cgtggtcaaa gttgcccaat gtgctgcaga ggaccaagaa ttattagatg
      361 aaaaagcaag catggttgtt gcaaaatatg atacatttga ataaagaaaa caattggacg
      421 tgcggcgtgc tgcaagtcca atttctttga gaaggtaaat cagtattctt ttcctatttt
      481 ctggagaaga aaaagcgagg cgagttgtca tggtaacatt ctttacggtt ctattgtttt
      541 tattcgttgt tgtgatgttt ttatttcttt ggctggttcg aaaagaaatt atttatcgga
      601 ctgtgcgaaa tcgatgggtc tacttggtga tacccttctt agtgtcacta gtcatttggt
      661 atacactcat cagtcatcct acagcagatg aattggcaaa agggatcttg tcagcaatga
      721 tttttattag ttttttgttg gacagtagag gaatcacaga agaaggactt gttttgaata
      781 gctttgacaa aaaaggtgtt cctttttctg agatcaataa aattgtatta tatcagccaa
      841 aaggttctaa aatcgtaaaa atgaattttt tccgaaacgg atggcgagga ccgatgctga
      901 aattttctgc atcattagaa gaattagttc catttttgtc ccaacgctta aacgatgagg
      961 cagagattga tattatgatt gatccagaat aaaaacagag gttgcgacaa gatgtttgtc
     1021 acaacctctg ttttttattt caaataaaat tatccaaata cgcggaagcg atcggcatcg
     1081 ttcatgtatt ctttttcccc agccggtgtt tctggtgaac cgaatactaa ttgcgaacgt
     1141 agtttccagt ttgaagggat attccattct tttgcaactg cttcgtcaat aactgggttg
     1201 taatgttgca agtttgcgcc caatccttta tcaactaatg ctacccaagt attagctgtt
     1261 gcgattccgt ttgattgttc tgaccaatct gggaagttgt ctgcatataa ttcaaattgt
     1321 tcttgtagac cttttaccac gtcagtatct ttgaagaata atactgtacc ataaccattt
     1381 ttgaaaccag ctaatttgtt ttgtgtattt gggaaagctt ctgctggagt caatgggcga
     1441 agtgcttctt cagtgatttc ccaaagtttt tcatgtgcat ctccgaaaag gataactgcg
     1501 cgtgttgatt gagcattgaa agcagttggg ctttctttaa ttgcttcttt gatcaaagtt
     1561 gttaattctt cattagaaag tgaaacatta cgtcctagat gatagattga acggcggttt
     1621 ttcaaagtgt cagtaaaagt tgtcataata aaatttctcc tttgatttgc gttttgcatt
     1681 tttttaattg attacgaaat gaagtatagc accttacaaa aagtaagtaa acagatatgc
     1741 tcacaaaaaa taagcgaagt tcaaaaaaca attttaataa gaaaaatcgt tatgta
//
LOCUS       DAIGOK010000216         1796 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.222, whole genome shotgun sequence.
ACCESSION   DAIGOK010000216 DAIGOK010000000
VERSION     DAIGOK010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.222"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            4..615
                     /gene="mobC"
                     /locus_tag="NOA01_002664"
     CDS             4..615
                     /gene="mobC"
                     /locus_tag="NOA01_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HCI0633808.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            600..>1796
                     /locus_tag="NOA01_002665"
     CDS             600..>1796
                     /locus_tag="NOA01_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HCI0633809.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETYKRLRIDPKDLERMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQDVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSNREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQ"
BASE COUNT          707 a          240 c          336 g          513 t
ORIGIN      
        1 tcaatggatg ataaacaaaa aatgtacgaa aaaaatcgca aacatagtga ggccttaaat
       61 tttcgtgtcg ataaaaagac ttatcttgaa ctgacattga ttgcaaaaca aaggggaatt
      121 tctagagcag acgtttgccg tgagcggtta aagggactca aatatgaacc gccgttgtta
      181 gctgaagatg atagggtagt tttaaccaaa cagttaaatc aaattggtaa caatttgaat
      241 caagcggtaa aggcattaaa tatcattgct aaatatttta gagaagaatc tgaagataaa
      301 aaagaaaaga gcctacattt tagttttgat gctagccgtt acaacatttt taaagatgaa
      361 cttgcgaata gcatagcaaa aaagttgatg attagttatc cagatgacat ttttttggag
      421 tccaatcgga gtgccccctc tttttttaaa ttatcagaag tagaaaaaga atatttcgaa
      481 tattggatag aaaagcaaac tagaccgggt acgaaagaag agtattccca aaaagttgat
      541 cggaatttaa ttcactcaat tcagattatt gcaagtttaa aagatgaggt gaaacaactt
      601 tgggagctac tataacatcg aaaggacgca agaaaaattt atcctcgcta ttacaatatg
      661 cagctaatcc ttcaaaaaaa gaaaaatgtg ttgcagtctc aggaataaat tgtagtgatg
      721 atagtgagga agcactgcaa gaaatgaatt tagtacgtac caagatgaat aaatctaaag
      781 atatagcaca agcgtttcat gttattcatt cgtttgataa gagtacttct aaagagttaa
      841 gtattgataa aatgcacgaa atagctgtcg aatttgcaga gaaagctttt ccaaattcgc
      901 aaatcattgt tgcaagtcac aatgataaag atcattttca tagtcatctt gtcattaata
      961 atattaacat ggaaacatat aaaaggttgc gtattgaccc taaagattta gaacgaatgc
     1021 gaaaaattaa cgatcaagtc ctcaatgaac atggtcttga atcagtgaat aatgagtact
     1081 atgaaaactt agaaaagaga accaatcttt attcaaacga tccttctaag atgaaatatg
     1141 gaaaaattaa ttatcaagag tttgtgcaaa atgcgatcga agcaacgttg gaaaaatgcg
     1201 aagtgaattc ttttgaagat ttttcggatc atttggcaaa agaatataac attgaagttt
     1261 atcaattttc aaaaacaaat aacaaacttg gctatgttct ttacaaacaa gatgtaaatt
     1321 tctatgagcg tagaggtgag ttaattactt taggaagaga taaacaatct agaaaagaaa
     1381 aaagtcagta tgtcgaacgt acttttagtg caagaaagtt agggaaaaga tattcatttg
     1441 aatctgtttc tgaacaactt gaaaagaatt tagaagaatc acatataaaa aattttttta
     1501 atatggaaag agagcaagtt atgtcgctaa ttagtggagt aaattacaaa actggtgagt
     1561 taaatgtacc gtttgtggaa ttagattcca tcgcacaaaa gcaatttagc aaccgtgaag
     1621 gcgatgaaaa attcattaaa tatgatgact tcttgcgaga ggtagacaag cattttgtaa
     1681 atacaatgtc tttagaagat gatcaagaat acacgattaa acttcatacg gatgtgaatc
     1741 ctcaaacttt acgtagtgcg aaaagtaaaa caatttctat taaaaagact gatcag
//
LOCUS       DAIGOK010000217         1758 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.224, whole genome shotgun sequence.
ACCESSION   DAIGOK010000217 DAIGOK010000000
VERSION     DAIGOK010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.224"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            137..1615
                     /locus_tag="NOA01_002666"
     CDS             137..1615
                     /locus_tag="NOA01_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCI0633810.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT          652 a          237 c          310 g          559 t
ORIGIN      
        1 tcaaaaaaaa gataaaaaat gagaataact gatgaaaaaa caagcataat ccatcataaa
       61 atcagtatag gtgaaaagat gaactttcat ctgcatatca agtaattggt atgaggcatt
      121 ttttgaggtg taggatatga agttggatta ccttatagaa gaggaagata gagaaaaact
      181 gctgctagct agatatttag aatttgagaa aaagaattat gtagtgctgc ctaagttata
      241 tgaaacatta ggtttgtcta aatttaaaac aagaaattat atcatagaat taaataaaga
      301 atttactgaa ttcaaaacaa agccagagat ccaaatttca gaagaagcag agatccatgt
      361 cgaacgtctg aatttgacgg atatcaaaaa tttaagaaga atctactttc aacgatcaaa
      421 aattgctcga ctgttaacgg agatcgtgga ttcaaaagta acgatcgaaa aatttgcaga
      481 gaatcatttt ttaagcatat caagagccta tgtaaaaaga agagaattag tctcttttct
      541 tgaacagcat caaataaagt taaagaaaaa catattagtt ggggaagaaa taaacatacg
      601 gaacgtatta tttggggtat atttcggggt ctttaatggt ttttctatgc catttagaga
      661 aagcgtcatg aatgaagcaa agagtatcgt gcaatatttt gaatatctgt tcaatttaaa
      721 actgtctgca acaaaacgaa tcaaattcaa tttgctaacc actattagtt tgttgagggc
      781 ctatgggaat caaccaatcg gaaaacgttt cttttcagat gaatacttta agagctcaga
      841 aggaatgaag atcatcaagg atttatcaca tatttctttt cttcatatcc ctaaagagca
      901 gatcattgat gagatttctt atatcatgtt gtttttgatc acggaagaag aatttgaatt
      961 agatggcgca tttggacttg cggaagctca ttttcatgat atccacatga ttagtcaaaa
     1021 aatgtcagtg gaattgattg atcaattacc attttcgatt gaaatctctg aagaagaaag
     1081 actatatata cgaaaagaac ttcgtatggg tatgatgcat gtcaatcgaa aaaattggat
     1141 attcgacttt gagatgacta gctttacgtc taaaaagcag ctacagtttt tcttcgagac
     1201 gtatccaata ttcagtacag caatccaaaa ggtcatacaa gagaataaac gctatttctt
     1261 gggaaaagat gaacgtttca tgattcgctt attttatgat tatctctttt tattggtcgg
     1321 tatttgtccg gtatcatatt ttgaagcacc gattcatgtc tgtattgatt tttcgcaagg
     1381 atctgcttat accgattaca ttatttctca agtgaaagga tttaaaaatt acaatattgt
     1441 gattgagagt cgaatcactt ctcgtacgaa tatttattta tctgattgtt tacttgaaaa
     1501 atttaataaa gaacagataa tttggaaaaa tccaccgact ccagatgact gggaatattt
     1561 tggaaatacc atcatcagag aaaaaaatag acatttaact gaaaaaatca gctagtattt
     1621 atggatggca gctttgtctc acatatttat tagctgattt atgttttttt caaagaaaag
     1681 gtaactagat ataactagat ataactagat atattactta acaaaattaa aaagtacata
     1741 agttattatt ccgatttt
//
LOCUS       DAIGOK010000218         1697 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.225, whole genome shotgun sequence.
ACCESSION   DAIGOK010000218 DAIGOK010000000
VERSION     DAIGOK010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.225"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            254..>1697
                     /locus_tag="NOA01_002667"
     CDS             254..>1697
                     /locus_tag="NOA01_002667"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCI0633811.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEE"
BASE COUNT          581 a          320 c          331 g          465 t
ORIGIN      
        1 atactataag ataagaaaac cactatttaa caaggttatt cgaaccttat tttaagttac
       61 ttattattta atgtatcgaa aaaggtgaaa taggtttgtt ataataagaa ttaagtaaga
      121 catgaattgt ctaagcaaat gtagcttatc catttcatta aaatacatat ggtgaaaaaa
      181 caagttcaaa tagtaactaa ttttttaaaa aactgattta taaaaaatac aacacgaatg
      241 aggaaaataa ccaatgacag aacaaatcga aaaaaattca aaaagcttat atcaggcact
      301 gtggaattcc gcagatatcc tgcgttcaaa aatggacgct aatgaataca agagctattt
      361 actaggcttg gtcttttata aatacttatc cgacaacatg ctgcggtatg tctccgttct
      421 attggaggaa gaaacggacg acctaaataa agcacaagac ctttacgttg aagcatttaa
      481 agatgcagat ataaaggatg atctcttgta tgaactaaaa gatgagtttt catataccat
      541 cgcacccgca ttgaccttta ccgcgcaagt ggcagccatt catgatggat cttttcaatt
      601 agaagacctg gtacaaggtt tccgtgacat tgaacaatcc agcgaaaact ttgagaactt
      661 atttgaagac attgatctgt attctaaaaa actggggtca accccacaaa aacaaaataa
      721 aaccatcgca gatgttatga aagaattaga agggttaaac atggcggggc atgctggcga
      781 tgtcttaggc gatgcctatg agtacttaat tggccaattc gcatctgaat ccggtaaaaa
      841 agccggtgaa ttctataccc cacaaccggt cgcaaaattg atgacacaaa ttgttttaca
      901 aggaaaagaa gataaaaaag gcttttctgt ttacgacgcg accatgggtt cgggctcgtt
      961 acttttgaac gctaaaaaat attcccatca accgggaacc atttcttatt tcggacaaga
     1021 gttgaatacg tctacctata atttggcgcg tatgaacatg attttgcatg gggttcccat
     1081 tgccaaccaa cacttgcata atgcggatac cttggatcaa gactggccaa cagaagagcc
     1141 aacgaacttc gatggggtgc tcatgaaccc accgtattct gcaaaatggt cggctgataa
     1201 aggcttctta gatgacccac gcttctctgc ctatggcgta ttagccccta aatcaaaagc
     1261 ggactttgct ttcttattac atggctacta tcatttgaaa gatacgggcg tcatggccat
     1321 tgttttgcca catggggtgc tctttagagg gggcgcagaa gggaagatac gcaaagcctt
     1381 gttagaaaat ggtgccattg acaccgtcat tggcttacct gctaacatct tctttaatac
     1441 gtccattcca acgaccgtaa ttatcttgaa gaaagaccgt gcgaaaaaag atgtgctctt
     1501 tattgatgcg tcacaagatt ttgaaaaaat aaaaacgcaa aatacgctac gagattacca
     1561 tattgacgct attttagagg cctataaaac ccgtacaatt aaagacaagt atgcttatgt
     1621 cgcagaattt gatgaaattg tggagaatga ttacaactta aacattcccc gttacgtgga
     1681 tacctttgaa gaagaag
//
LOCUS       DAIGOK010000219         1666 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.226, whole genome shotgun sequence.
ACCESSION   DAIGOK010000219 DAIGOK010000000
VERSION     DAIGOK010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.226"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            133..507
                     /locus_tag="NOA01_002668"
     CDS             133..507
                     /locus_tag="NOA01_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HCI0633812.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(621..1250)
                     /locus_tag="NOA01_002669"
     CDS             complement(621..1250)
                     /locus_tag="NOA01_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCI0633813.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1384..1456)
                     /locus_tag="NOA01_002670"
     tRNA            complement(1384..1456)
                     /locus_tag="NOA01_002670"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1421..1423),aa:Thr,seq:ggt)
BASE COUNT          584 a          240 c          284 g          558 t
ORIGIN      
        1 gtctacactc tgagatgtaa gaaatatcat aagatatttc ttacatcttt ttgttttgag
       61 caggacttta gataatttta ttaatatatg ctagcatgct tttgtaagaa aaagaaagag
      121 ggcgtattag ttatgtatct taaaattacg gaacaagcac aagaaagatt acaaaaatat
      181 attgatgaag gcgcaacagt tatattggat ttagatgatg gcgttgggga atattctaaa
      241 atgggtgttt gctctttaga tactagtttt cgtttacttc ttcttgataa agaacagaga
      301 aaaaatgatt ataaactcca attagatagt gatattgggg atgtttatat aaaagattat
      361 tcaaaaatgt acatggatga acatatgacc ctttctttag atccacgtct aggtgtattt
      421 actcttgaaa gcccaagtgg ttctttagac agtcatgttc aattagtaga tctacgacca
      481 gaaaaattct ctggaataaa acaataactt tctctctatt gtccataaaa ctagtctcaa
      541 tcaagctgta ttgaaaaaac aagacaacat tcttaccatg gtaaataaaa agacgacaca
      601 ttttgtcgtc tttttttaaa ttaactattt tcgataaaag atcgcatagc ttgtgccatt
      661 atttctggct tttctctatt caattcatgt tttccgtttt taataaagag tagccggcca
      721 ttcgttgaaa gtttatataa acgttttgct gctggtttat tcgcatgatc tttggtaccg
      781 cataaaatca atttaggaag tcgtgatgta cgaagctgtt ttttgcagtc taatgtttca
      841 agtgaacgaa gtagggtaag ggtttgtttt ttagatagac ctaatttttc aaatatctta
      901 gaaggtacgc agtaaaaata aatattctgg aaagtaagca ataagcgatt aggagactca
      961 aattgtggag cgactaaaac aacctttttt aaatatggag aaggagttgc aagatagttt
     1021 aaagcaataa cagcgcctaa agataatcca cataaaataa aaggttcctt tatctgattc
     1081 aattgtactt cgatgtattt ggttaagtcg tctaaagtag cagtgtctgt gctgtttaaa
     1141 tgattgaaca atgaaagact tttgcaagaa taactattgg gtagataaga gattacatct
     1201 ttccatgatt gttcgtcttg gcctagacca tgcaaaaaaa caattttcat attgctctcc
     1261 tttttatagt tatcatatag actttctttg tactattaaa atataaacat tcatttctta
     1321 cgtcataaaa taaaaaaaca gcgcagacac attgttaaac caacatgttg cactgtttaa
     1381 ggaagccact tgccgggctt gaaccggcga cctcttcctt accatggaag cgctctacca
     1441 gctgagctaa agcggcatta tcattaatac aaataatagg ataacgaata ttaatgtatt
     1501 tgtcaacaga attttttcga aaaaatgtgt ttctctaata ggaaaacaga ttttagaaaa
     1561 aataaaaata acaaaaaatg cgaacggttt ctttgataat ataataaaaa aattcaaaaa
     1621 aagtattgcg ttatttagaa gaaattgata ttatattcat gtactt
//
LOCUS       DAIGOK010000220         1626 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.227, whole genome shotgun sequence.
ACCESSION   DAIGOK010000220 DAIGOK010000000
VERSION     DAIGOK010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.227"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(<1..113)
                     /locus_tag="NOA01_002671"
     CDS             complement(<1..113)
                     /locus_tag="NOA01_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HCI0633814.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEK"
     gene            complement(266..592)
                     /locus_tag="NOA01_002672"
     CDS             complement(266..592)
                     /locus_tag="NOA01_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633815.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(604..804)
                     /locus_tag="NOA01_002673"
     CDS             complement(604..804)
                     /locus_tag="NOA01_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCI0633816.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            927..1529
                     /locus_tag="NOA01_002674"
     CDS             927..1529
                     /locus_tag="NOA01_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCI0633817.1"
                     /translation="MEFTIGGVTLNRIKFYIDSSKKTIKQISTETGINYTTLTNYYRG
                     ERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIKTARLEKKDKIVKNIAS
                     LDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSFEYTILFAKQKKFINKA
                     LNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
BASE COUNT          542 a          276 c          203 g          605 t
ORIGIN      
        1 tatttttcat ttacgtttaa aaaaatagga tattgatagt ataattctgc atatatatca
       61 gaattagaat agccttgctt ttcaactaag ctttcaactt ctttttcatt cattgtcaca
      121 cctgcttctg attttatttg ctataataga ttcaaataaa gttttctcag aactttatta
      181 ttgagtgttc ccccgatctg caaactgacg ggaacactct ttttaatttt ttctacttca
      241 tcaaatttaa aaaacgaggt gttttttaaa tttgatgact caatatccat tttttgcatt
      301 cttcttttga aaacaactcc attttttcta ataaaataac tggcaatccc attcttttaa
      361 atttcaaaaa agttgtatat gatacattca aatatttagc catctctttt ccttttagaa
      421 atggtctttc taatccggta tcaattcttg cttgatcaat agcctctttt gtcatttcaa
      481 aaacataatt tttcaattga ttcgtttgtt gtttatctaa cattactgta attcctgcat
      541 ccattatttt tctcctctca ttttctattt cttcaatcag ccaactcgcc aagttgtatt
      601 tatttagatt ttcaaatcat taagtaacca atttaccaac ttttgataag tagatgctct
      661 aatactttct ttgctcccat tttcgatttt gacatatgtt ttccgattta tacctatcaa
      721 ttttgctgca tcgtttttag taagtataag atcggctcta tgtcttctta agcttctaat
      781 gaattgatca ttaagagtta tcattcagcg cctcctttca aattattcac tttgtggttt
      841 ttatcttaat aagcacattt taaaccacaa aacgagtaat atcaattaaa atattactca
      901 atttgtggtt ttcatgctat aatagtttgg aatttactat agggggcgtt actttgaata
      961 gaataaaatt ttatattgat tccagtaaaa agaccattaa acaaattagt acagaaactg
     1021 gtattaacta tacaactctt acgaactatt accgtggtga gcgaaatcct agaaatactg
     1081 aaacatggaa tatccttgct gattatttcc atgttcctgt aagttatttg atgggattag
     1141 acgaaacaaa ttccatagaa caacctaatc ttgatagaat aaaaactgct cgtttagaaa
     1201 aaaaggacaa aatagtcaag aatattgcca gtcttgactc agattatttt actgaattat
     1261 ctaatctata cttttccatg acttctttac ttgaagtcaa acttcaagaa gatgactccg
     1321 actttctttt tttaaaaagt attctagagg actcctttga atacacgatt ctatttgcca
     1381 aacaaaagaa gtttattaat aaagctttaa acttaaatat cgatgaaaga aaagagttag
     1441 aatctgagtt atttggcaac ttaacccaag tacaggttaa caatctaaaa agagagataa
     1501 cagctcgatt aatatcaaaa cttcaataaa actaattatt caatcagcca actcgccaag
     1561 ttatcagatt ggagagatta gttatgtcaa tacaaaagta cacaacaaaa agcggtgcac
     1621 gatata
//
LOCUS       DAIGOK010000221         1584 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.228, whole genome shotgun sequence.
ACCESSION   DAIGOK010000221 DAIGOK010000000
VERSION     DAIGOK010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.228"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            292..1479
                     /locus_tag="NOA01_002675"
     CDS             292..1479
                     /locus_tag="NOA01_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCI0633818.1"
                     /translation="MYFLGIDIGKRTHVASIMNEEGKVLLKGFSFPNTTEGAESLIER
                     MVDYSGAPSDFVIGMEATGHYWLSIFSYLHESDYLIHVVNPLQTDGWRKGTEIRKRKN
                     DIIDSVLIADLMRYGSFVETVLSDENVFSLKQLSRYRTYLVGTASDFKRKIIAVLDQV
                     FPEYATIFTKQGVFGKASKELLLAFSAPIDLENISSETLAQYLAELSRNRIKAEKADQ
                     LKQAASNSFGVKFAQEAFTFQLRSMIEQLKFIESQIKETEREIKHIMDTLNSVILTIP
                     GIGPINGATILGEIGDIQKFSNPKKLVAYAGIDASVTQSGQYEATHNVMSKRGSPYLR
                     KALFSAALVASQCDPVFSAFYQKKVSEGKHHLTALGAVSRKLCYVIHAILTKNEPYII
                     VSD"
BASE COUNT          467 a          296 c          316 g          505 t
ORIGIN      
        1 ttttaacctt ccggttttga ttggataacc ctatgaacca tcgttatttt aatttggtat
       61 taactcattt cttttgagat aatatattta ttggattgag aggctaagcc gttcccccct
      121 tgggccattg aacccgcact cgagagactg gctcctctct ctgataagtg gattgcgtat
      181 gagttagatg ttgaaagtcc ttactttact ccgtatctcg tacaaggtag tgacgctttt
      241 cagttgagtg gatttcaatc caaaattctt tttttgaaag gtcagatata catgtacttt
      301 ttaggcattg atatcggtaa acgcactcac gttgcttcta tcatgaacga ggaaggaaag
      361 gtattactaa aaggtttttc atttcctaac accactgaag gagcagagtc attaattgag
      421 cgaatggtcg actattccgg agcacccagt gactttgtta tagggatgga agccacgggg
      481 cactattggc tgtccatctt ttcatacctt cacgaatcag attatttgat tcacgttgtg
      541 aatccccttc aaacagacgg atggcgtaaa ggtacagaaa tacggaaacg aaaaaacgat
      601 attattgatt ctgttttgat tgctgactta atgcgctatg gctcatttgt cgaaacggtc
      661 ttatccgatg aaaatgtttt ttctttgaaa caactttctc gttaccgaac ttatttagta
      721 ggtacagcga gcgacttcaa aagaaaaatt atagccgtat tggatcaagt ctttccagaa
      781 tacgcgacga tttttaccaa gcagggcgtt ttcggaaaag cttctaaaga gttattgctg
      841 gcgtttagtg ccccgatcga cttagaaaac atttcctcag aaactttggc ccaatactta
      901 gctgagttaa gcaggaaccg aatcaaggca gaaaaagcgg accaattgaa gcaagcagct
      961 tctaactcct ttggagtaaa atttgctcaa gaagcgttca ccttccaact acgttcaatg
     1021 attgaacaat taaaattcat tgaaagccaa ataaaggaaa cggaaagaga aatcaagcac
     1081 ataatggaca ctttaaattc tgttattctc acaattcctg gaattggtcc tataaacggt
     1141 gcgactattc ttggtgagat cggagatatt caaaaatttt ctaacccgaa aaaactcgtt
     1201 gcttacgctg ggatagacgc ttccgtgaca caatcagggc aatatgaggc aacgcataat
     1261 gttatgagta aacgagggtc tccatatttg cgaaaagcct tgttttcggc ggctttagta
     1321 gcttctcaat gtgatcctgt ttttagtgcg ttttaccaga aaaaagtttc cgaaggaaaa
     1381 catcatctaa ctgccttagg tgcggtttct cgtaagcttt gttacgtgat tcatgctatt
     1441 ttgactaaaa acgagcctta tataattgtt tctgattagc tattgaacat tcaaaaaaac
     1501 taatggtaag aagcttattt gttgtaccct tttttcggaa ttatctgtta taaaatattt
     1561 tttgatttgg ctaattttta cttg
//
LOCUS       DAIGOK010000222         1539 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.229, whole genome shotgun sequence.
ACCESSION   DAIGOK010000222 DAIGOK010000000
VERSION     DAIGOK010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1539
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.229"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(52..1518)
                     /locus_tag="NOA01_002676"
     CDS             complement(52..1518)
                     /locus_tag="NOA01_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HCI0633819.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT          502 a          326 c          289 g          422 t
ORIGIN      
        1 aaaaaacagg aactgctgca aataagcaat tcctgttttt atgttcattg attaatgaat
       61 agagcccatt agttttttga ttggttcttt cacagtaaat agcaagatac caccgacgat
      121 tgctactgca ccgataatac cgaagtaagc agattctgtt cccggtgtgt agaagcgtgc
      181 aatttgtgca ttgattgctt gtgaagaagc atcagctaag aaccaaatag cgattgtttg
      241 tgattcaaat gctcgtggtg ctaattttgt tgtcacagac aaaccaactg gtgaaagaca
      301 caattcagca ataaccacga tgaagaaact tcccaacaac cacaatggac ttacttttcc
      361 acttgtacca taaagcatac ccggcaacat caatagcaag tacgataatc ctgcaaataa
      421 aagtccaaga gagaatttca ctactgtaga tggttgacgt tcaccaagtt tcgtccacat
      481 cgttacgaat actggtgtca gaataaagat gaaaattgga ttcaatgatt gataccagct
      541 tgcttggatc gttacaccaa atagcgtaga ttgtgtacgt tcactagcaa ataaagctaa
      601 gatagatgaa ccttgttctt gtaatgacca gaaaatgatt gctgcaagga ataatgggat
      661 ataagcccat actcttggtt tttcttcagc agttacatct tttgaagtca gcattttgat
      721 aaagtaatac aacggtaaga taatccccaa tacactgatt gtattgacaa aaaagtcaat
      781 tgtcagatga ccagttagtt gtgccccgcc gaatactgca gcgataacaa caagtgcgat
      841 gatcaagtta cggatgaatc ctttacgttc ttcagttgtc aaaggattcg gcggtgttgt
      901 acccactccg tctaatgtct tgcgtccttg gatgatgtat tgtaataatc caaagaacat
      961 cccaatagca gctaaagaga accctaaatg atagttatat tcttgaccga ttgtaccaac
     1021 gattagtggt gctaataatg cccctaagtt gacacccatg taaaaaatcg aaaatcctgc
     1081 atctcttcgc aagtcagtct ttgaataaag atgtccgacc atgccagaaa catttggttt
     1141 caaaaaggcg gttccgataa taatcaatgc catcgaaaca aacaatgtac ttacaccaaa
     1201 tggtaaagcc aagacgatgt ggccaagcat aatgaaaact ccaccgaaga atacagtgcg
     1261 ttttgctcct aacactcggt cagataccca tccgccgacg atactagaca tgtagatcaa
     1321 agatccgtag atcgacatga tagctaatgc tgttgctcgt ggtaatccaa gtcccccgtt
     1381 ggcaactgta tcgtacatat aatagataag taatgcacgc ataccgtaat aactaaatcg
     1441 ttcccacatc tcagtgaaga acaaagttga caatcccttc ggctgcccga agaagccctt
     1501 gtcttgctgt ttttccataa tctatacccc aattcttta
//
LOCUS       DAIGOK010000223         1508 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.230, whole genome shotgun sequence.
ACCESSION   DAIGOK010000223 DAIGOK010000000
VERSION     DAIGOK010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.230"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(74..158)
                     /locus_tag="NOA01_002677"
     tRNA            complement(74..158)
                     /locus_tag="NOA01_002677"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(122..124),aa:Leu,seq:caa)
     gene            complement(176..247)
                     /locus_tag="NOA01_002678"
     tRNA            complement(176..247)
                     /locus_tag="NOA01_002678"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(213..215),aa:Gln,seq:ttg)
     gene            complement(257..329)
                     /locus_tag="NOA01_002679"
     tRNA            complement(257..329)
                     /locus_tag="NOA01_002679"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(294..296),aa:His,seq:gtg)
     gene            complement(391..475)
                     /locus_tag="NOA01_002680"
     tRNA            complement(391..475)
                     /locus_tag="NOA01_002680"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(439..441),aa:Leu,seq:caa)
     gene            complement(496..566)
                     /locus_tag="NOA01_002681"
     tRNA            complement(496..566)
                     /locus_tag="NOA01_002681"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(532..534),aa:Cys,seq:gca)
     gene            complement(612..683)
                     /locus_tag="NOA01_002682"
     tRNA            complement(612..683)
                     /locus_tag="NOA01_002682"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(649..651),aa:Gln,seq:ttg)
     gene            complement(695..767)
                     /locus_tag="NOA01_002683"
     tRNA            complement(695..767)
                     /locus_tag="NOA01_002683"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(732..734),aa:His,seq:gtg)
     gene            complement(770..843)
                     /locus_tag="NOA01_002684"
     tRNA            complement(770..843)
                     /locus_tag="NOA01_002684"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(809..811),aa:Trp,seq:cca)
     gene            complement(850..930)
                     /locus_tag="NOA01_002685"
     tRNA            complement(850..930)
                     /locus_tag="NOA01_002685"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(894..896),aa:Tyr,seq:gta)
     gene            complement(932..1007)
                     /locus_tag="NOA01_002686"
     tRNA            complement(932..1007)
                     /locus_tag="NOA01_002686"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(972..974),aa:Phe,seq:gaa)
     gene            complement(1043..1115)
                     /locus_tag="NOA01_002687"
     tRNA            complement(1043..1115)
                     /locus_tag="NOA01_002687"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1080..1082),aa:Asp,seq:gtc)
     gene            complement(1125..1197)
                     /locus_tag="NOA01_002688"
     tRNA            complement(1125..1197)
                     /locus_tag="NOA01_002688"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1162..1164),aa:Val,seq:tac)
     gene            complement(1213..1284)
                     /locus_tag="NOA01_002689"
     tRNA            complement(1213..1284)
                     /locus_tag="NOA01_002689"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1249..1251),aa:Glu,seq:ttc)
     gene            complement(1299..1388)
                     /locus_tag="NOA01_002690"
     tRNA            complement(1299..1388)
                     /locus_tag="NOA01_002690"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1352..1354),aa:Ser,seq:gga)
     gene            complement(1428..1501)
                     /locus_tag="NOA01_002691"
     tRNA            complement(1428..1501)
                     /locus_tag="NOA01_002691"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1465..1467),aa:Asn,seq:gtt)
BASE COUNT          439 a          390 c          340 g          339 t
ORIGIN      
        1 atttggaggt aattacataa aaacaaaaaa aatatagcaa ctctctataa gaaagttgct
       61 atattgataa ttataccggc ggccggggtc gaaccggcac gtcctcaacg gacactggat
      121 tttgagtcca gcgcgtctgc caattccgcc acgccggcaa atttaaaaat aataactggg
      181 gtagctggat tcgaaccaac gcatgaggga gtcaaagtcc cttgccttac cgcttggcta
      241 taccccaaga ataaagggcg aatgatggga atcgaaccca cgaatgccgg agccacaatc
      301 cggtgcgtta accacttcgc cacattcgcc atcataaaag atgttattta atttctcaat
      361 aaaaaactaa gatgaagaca tcttagtcaa taccggcggc cggggtcgaa ccggcacgcc
      421 ctcaacgggc actggatttt gagtccagcg cgtctgccaa ttccgccacg ccggcaagtt
      481 gatttattag tactaaggcg gtaaccggat ttgaaccggt gatgaaggtt ttgcagacct
      541 ctgccttacc acttggctat accgccatta tactattatc aatctataaa ataaagaata
      601 aattatagaa actggggtag ctggattcga accaacgcat gagggagtca aagtcccttg
      661 ccttaccgct tggctatacc ccaagaataa aaagggcgaa tgatgggaat cgaacccacg
      721 aatgccggag ccacaatccg gtgcgttaac cacttcgcca cattcgccat ggcaggggca
      781 gcaggaattg aacccacact aacggttttg gagaccgctg ttctaccttt aaactatgcc
      841 cctataaaat ggaggagagt ggattcgaac cactgaaccc taaggaacgg atttacagtc
      901 cgtcgcgttt agccacttcg ctactcctcc atggtggcgc gggacagaat cgaactgccg
      961 acacacggag cttcaatccg ttgctctacc aactgagcta ccgcgccata ttttaaaatt
     1021 attaattgaa taacaagaac gacggttccg acgggaatcg aacccgcgat ctcctgcgtg
     1081 acaggcaggc atgttaaccc ctacaccacg gaaccagttc attatggagg ttaacgggat
     1141 cgaaccgctg accctctgct tgtaaggcag atgctctccc agctgagcta aacctccaac
     1201 attcaattaa aatgacccgt acgggattcg aacccgtgtt accgccgtga aagggcggtg
     1261 tcttaaccgc ttgaccaacg ggccaggtaa atttgctacg gagagtaagg gattcgaacc
     1321 cttgagacag tttggcacca tctacatgat ttccaatcat gctccttcgg cctctcggac
     1381 aactctccag agtgaaagaa gctcaatact ctttctcaaa atgagaactc cggcagtagg
     1441 actcgaacct acgacatcat gattaacagt catgcgctac taccaactga gctatgccgg
     1501 aataacat
//
LOCUS       DAIGOK010000224         1490 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.231, whole genome shotgun sequence.
ACCESSION   DAIGOK010000224 DAIGOK010000000
VERSION     DAIGOK010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.231"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(83..155)
                     /locus_tag="NOA01_002692"
     tRNA            complement(83..155)
                     /locus_tag="NOA01_002692"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(120..122),aa:Gln,seq:ctg)
     gene            complement(201..629)
                     /locus_tag="NOA01_002693"
     CDS             complement(201..629)
                     /locus_tag="NOA01_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633820.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(689..1447)
                     /locus_tag="NOA01_002694"
     CDS             complement(689..1447)
                     /locus_tag="NOA01_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HCI0633821.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
BASE COUNT          430 a          284 c          277 g          499 t
ORIGIN      
        1 agtagtaaaa ataatacaat aatccccctg caaaaagatt gttcttttac agggggttca
       61 ttttttcagc aagcacatca aattgggatg gcagggttcg aacctacaat tgcttgattc
      121 agagtcaagt gccttaccag tttggctaca tcccatttat tcaacaaaat aatcatagca
      181 ctttcctaaa aaaacgcaag ttatttttct tgatcggata gttgttgctt tactcgtaaa
      241 aggcggcgta ctagtttctt ttctaatcca gcaggaaggg caatcagttc tgctactgac
      301 aagacggtgt ccaataactg atattctttg acaggttcat tcataatatg atagatgttt
      361 gctaaacgtt caatatattc aaagcgagta tactctgttc gagcaattag aaatttcaat
      421 aaatcggtgg ctttctcgta ctcttgatct gcgtaataaa caagagattg acgatacaat
      481 ttgatttcca gttcattgag ggtagcaggt ctgcgacgct cgttccactc attaatagag
      541 actttacctt gaacatttcc cttgcagtca cggacaaaaa taatcggaat ttcatttgga
      601 tccatttttt tatgtgtgaa tttttccatt atctacgctc ccttcaaagg tttggtatct
      661 tgagtataag ggatttttgt ttgaattact agcttttctc caaagttagc atctgtccac
      721 ccattgcagt gaaaatcgcc gaaaaatcgt tgatcggtac tgctcgattt tttacaccat
      781 agggatcgtt gacataaata ttttctttgt catatccagt gactacacaa gcatgaacaa
      841 gaggagtgac tcttacttct ccattatttg tggtccaagt cataaaatca gtttcatctg
      901 ggacttcaaa attgatagtc acagaagtcc agacaggatt tcctttagat agtgcttttt
      961 tgatatcatc gagttgagca tcggtttttg gaaaagtgtg ataatccgtt tggacgattt
     1021 gctgagcaag ggaggcaact gggggaacat agacgaccat agcccagtct ccgccactga
     1081 tatcaccaac aaatccttca ttaggatcgc catgagtccc atctgcattg aaaacaggga
     1141 cgtaatccaa ttgctcagct aactggtttt tagttgtttc ataaccataa aaagataaaa
     1201 gcatagaaag cgaagtgact tcacagccat tttctaaaga aggagaatca aactggctct
     1261 ctaatggaac atctagtaaa acttctgctt tctcttttgt ctttttcgtt ttttgtgttg
     1321 aacttttttg tgcagtgttc attgtatgtt ctggagaatg gatatgtagg taataacttg
     1381 taccgcttaa taaaagagca gctgtgcccc ctagaaacaa tagttttttg aacgtttttt
     1441 tcttcatttg aaatctccct tttcagctgt ttatctttcc tattataaat
//
LOCUS       DAIGOK010000225         1481 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.232, whole genome shotgun sequence.
ACCESSION   DAIGOK010000225 DAIGOK010000000
VERSION     DAIGOK010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.232"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..1127
                     /locus_tag="NOA01_002695"
     CDS             <1..1127
                     /locus_tag="NOA01_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCI0633822.1"
                     /translation="SVRLYVRKDENGKQEYYVKKGFKTEKEAKLHEARKKVEIEDTGY
                     HKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFGNKSISKITPVHCQN
                     AVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPKREKKENENYFSLEE
                     LKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFEEQLMYVRKSYATIR
                     NEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQARELLQFGINTNTKN
                     QLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFRHTHATLLLEGGASI
                     KETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
BASE COUNT          553 a          235 c          240 g          453 t
ORIGIN      
        1 atagtgtccg cttgtatgtt cgtaaagatg aaaatggaaa gcaagaatac tatgtaaaaa
       61 aagggtttaa aactgaaaaa gaagccaagt tacacgaagc aagaaaaaag gtcgagattg
      121 aagataccgg ctatcataag ccttctaatg acttgtttat aacagtgtat gaagaatggt
      181 tgccttctta taggaatcgt gtacaagaaa cgacatatca acgtacaaaa gaccttttcc
      241 gcttgcatat actaccaaaa ttcgggaata agtccatttc taagattact cctgttcact
      301 gtcagaatgc agtcaatgaa tgggctttaa aattcaagaa tatcaaacag cttaagtctt
      361 acagctctca aatatttgag tatgccattt ttgcagagct attacaccga aatcctatgg
      421 caaatacaaa tctaccaaag cgggaaaaga aagaaaatga aaattacttt tcattagaag
      481 aactaaaagc tttttgggat attctcaata aagaagagcc tttaaaacat attttaatct
      541 tccagctttt gattactact ggtatacgta aaggagagct agctgctctt cattggtctg
      601 acatttattt tgaagaacag ctaatgtatg ttagaaagag ctatgcaact attcgaaatg
      661 aagagaagaa tgcaagaaga aaaactgtca ggattcaaaa aaatacaaaa aatatatctt
      721 ccgaacgaat attaccaatt gatgcacaaa ctattgaaat gttaaaaatc tggaaaaaag
      781 aacaagctcg agagcttttg cagtttggca taaatacaaa tacaaaaaat caacttatct
      841 ttacctatgt aaatgcagac ggtgacatta atcaaccttt gcatgcggac tattcaaata
      901 atatattgaa acgtttagag aagaaatata aatttaagca cgttactatt catggattcc
      961 ggcacactca cgctactctt ctactagaag gtggagcaag tatcaaagaa acacaagatc
     1021 gattgggtca taaaaatgct gaaactaccc ttaacactta ttctcatgtt actgaaaaag
     1081 ctcaacgaaa tgcgttagaa aaattcattg cttacactgg tttataatat tgtcattacc
     1141 tttctcatta ccagaacgaa aaaaagacac cttctcgcaa tgagaaagtg tctgaaatcg
     1201 ttggtataac aacgattaac gttttgaaaa ctgtgaagct ttacgtgctt tcttaagacc
     1261 tggttttttc ttcgtatcat tgaataagtt tgaaaaacct tgatttataa aggttttccc
     1321 gtaaatatat tttattatat ttgattaaat ttgaaatgtt tgtgttctaa aatgtgttct
     1381 aatttaaagg cagtatattt cgtattttag attaaatttt taggctcatt cattctcaaa
     1441 tccattctat tcagtcaata aaaattttaa aaagacttta t
//
LOCUS       DAIGOK010000226         1466 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.233, whole genome shotgun sequence.
ACCESSION   DAIGOK010000226 DAIGOK010000000
VERSION     DAIGOK010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.233"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            50..1438
                     /locus_tag="NOA01_002696"
     CDS             50..1438
                     /locus_tag="NOA01_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HCI0633823.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDRE
                     IRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVD
                     IMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTI
                     FGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEGTIKDEEKYIKMSKKIY
                     ITEFDDFNDYPF"
BASE COUNT          582 a          179 c          247 g          458 t
ORIGIN      
        1 ttcaaaaaaa aaccaaccga aagatataaa agatattgta gatacacgaa tgtataaaga
       61 acgtgatgac ttaaactata cgcttattat caataaagat ggaaactatt catggcgtcc
      121 tctcgttcta ctacatccta ttctatatgt agatttggtt aattgtctca ctaaagaaga
      181 ccattgggag gagttaaaaa agaggtttag tgaatttaaa aaagataaaa atattaactg
      241 ttattctata cctgttgaag cttccaaagg ttcaaaaagg acagatcttg gtgaaacaat
      301 actaaactgg tgggaatatt ttgaacaaaa aagcttagct cagaatatta aataccaata
      361 ttgcatgttt actgacatcg ctgattgtta tccatcaatt tatactcact ctataacatg
      421 ggccatgtat ggtaaagaaa aagttaaaaa tgatgaaaat aagtgttcgg gctcacttgg
      481 cgataaaata gataaaaaaa tatctggaat gcaacacaat caaacaaatg gtatccctca
      541 gggttccgtt cttatggatt ttgtagcaga gatcgttcta gggtatgttg atttgagatt
      601 atcgaaagaa ttaaaaaaat tagaaaattt aaaagattac aaagtactca gatatagaga
      661 tgattataga atattttcaa ataatcgtaa tgatttggaa caaattatga aagtgttatc
      721 agaagtctta tatgatttaa attttaagtt aaattctagt aaaacaaaat tatcagatga
      781 tattatcata gattctatca aaaaagataa gctctattgg gaagaaatta aggcaagtat
      841 aagaatgaat gccacaaact tagatggtga tagagaaatt cgttatagta taaatttaca
      901 aaaacattta ttggaaataa aaaagttagc agataagcat cctaattcag gccaattgaa
      961 aaaagcactt tctgagttta ataaagaacg cattattaaa atcacttcaa agccgaacga
     1021 tcttttacca cttattggta tcctggtaga tatcatgata aataacccgg gttctttgcc
     1081 gcagtgtgtc ttaattatag ggcagttatt aaatttcgaa aatgagagta aggctagaga
     1141 aatagttgaa atgattataa acaaatatgc tttaaagcca aatactgaat ttatgatgat
     1201 atggtttcaa agattgacca ccatttttgg agaaatgttt gatagtgttt tatcatccga
     1261 atttgtatct aaaatcaaag atcctgtctc taatcagatg ttttctgtag actggctaaa
     1321 tgaagatgct aagaaatctt tttatgaagg gaccattaaa gacgaagaga aatatattaa
     1381 aatgagtaag aaaatatata ttactgaatt tgatgatttt aatgattatc ctttttaagt
     1441 gaaaatttag aatgaatata aaaaac
//
LOCUS       DAIGOK010000227         1419 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.234, whole genome shotgun sequence.
ACCESSION   DAIGOK010000227 DAIGOK010000000
VERSION     DAIGOK010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1419
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.234"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(<1..210)
                     /locus_tag="NOA01_002697"
     CDS             complement(<1..210)
                     /locus_tag="NOA01_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCI0633824.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDL"
     gene            complement(309..659)
                     /locus_tag="NOA01_002698"
     CDS             complement(309..659)
                     /locus_tag="NOA01_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633825.1"
                     /translation="MIFENESEEIGRVAEENKVDPLETLVINKKEFEQLFLDFFIQYD
                     MIYDFSSSLDLFELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFR
                     EFRNKIIGFSQTTK"
     gene            complement(700..1194)
                     /locus_tag="NOA01_002699"
     CDS             complement(700..1194)
                     /locus_tag="NOA01_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633826.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYAMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRANGYFVGL
                     PFTL"
     gene            complement(1187..>1419)
                     /locus_tag="NOA01_002700"
     CDS             complement(1187..>1419)
                     /locus_tag="NOA01_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633827.1"
                     /translation="FGSKANMFRVMVSNLPVLASPSNNKFNDPESIKFEQKISELESM
                     ISNEVIEKLDDIDQKLSYSLKNKYKTEEKKDV"
BASE COUNT          446 a          262 c          168 g          543 t
ORIGIN      
        1 taaatcagat acataagccc atgtacccgt tcgttgtaaa taaagttctt cttgtaacaa
       61 ctcccatttt ctcttagaaa tcataatgac gccatcgttc aatccatttt tagaatccct
      121 agtaatttct atttcagaat ttgaatgaac tacttcttgc aacactttac ctatattttc
      181 tttgaagttt gttggagtaa tagtatccaa aatgaatacc tctcttctct atacctctat
      241 tatgaagtgc ccataaaata aagcaagcgt tgaggaataa ttctatcaac acttgcttta
      301 ttaagctttc attttgtagt ttgactaaaa ccaataatct tatttctaaa ctctcgaaaa
      361 acttcttgtg gttgataata gtctggatct atttcaaatg tttgtgagaa taattcattg
      421 aatttttcta ctgaaataat gattttacca ttcttctctc gactgttttc tattatttct
      481 gcttctaatt caaataaatc tagtgacgaa ctaaaatcat aaatcatgtc atattgaata
      541 aaaaaatcaa gaaaaagttg ctcaaactct ttcttattta ttactagtgt ttcaagcgga
      601 tcaactttat tttcttcagc cactcttcca atttcctcac tttcattttc aaaaatcaca
      661 aaaatgatat taacttaaaa gccactaaca aaataagact tacaaagtaa agggtaatcc
      721 aacaaaataa ccattcgctc tacgtactac gatttggtag tcctgccttt caatcggtaa
      781 ttcataatag taatttactg tttcttcttt gccactttca atatactctg gattatccaa
      841 tatcatagca tactcataaa gttcttcacc atccttgttt tcaatcgacc attgttgtgg
      901 acgtatcgga ttttctgtat catttataag tgtataggtc acttgtaata cacgatcatg
      961 ttgtgcaaat tcatttcttt tttcggtcac ttcaatattt ttaaatgtta gttcaacccc
     1021 atctgcctga taagtttcac ctatttttaa atcttttgtt acaattttta ttggcttttc
     1081 ttctggaaaa tgtctggctt taattgttag ataacttcca aaaagaatca atcctagtag
     1141 cactaaaaga attcctttaa cgagccactg aaacgggaag taaactttaa acatcttttt
     1201 tctcctccgt tttgtatttg ttttttagag aatacgataa cttttgatca atgtcatcca
     1261 gcttttcaat tacttcatta cttatcattg attctagttc tgaaattttt tgttcaaatt
     1321 taatgctttc aggatcatta aatttattat ttgatggact agctaacact ggtaaatttg
     1381 aaaccataac acgaaacata tttgcctttg aaccaaaaa
//
LOCUS       DAIGOK010000228         1397 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.235, whole genome shotgun sequence.
ACCESSION   DAIGOK010000228 DAIGOK010000000
VERSION     DAIGOK010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.235"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            12..422
                     /gene="spx"
                     /locus_tag="NOA01_002701"
     CDS             12..422
                     /gene="spx"
                     /locus_tag="NOA01_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCI0633828.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            436..792
                     /locus_tag="NOA01_002702"
     CDS             436..792
                     /locus_tag="NOA01_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HCI0633829.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          511 a          209 c          263 g          414 t
ORIGIN      
        1 ggagtgagaa aatgataaaa gtatatactt cgcctagctg tacttcctgt agaaaagcaa
       61 gagcatggtt gcaagcgaat caacttgaat ttgaagagaa gaatattttt gccgaaccgt
      121 taacagaaag tgagatcaaa aagatcttgg cagcgactga aggaggagtt caagatatta
      181 tttccactcg gtcgaaaatt tacgaaaagc tgggcataga ttttaacgaa ttaactttaa
      241 agcaaatgat cgtcttattc aaagaatatc cttcattatt gagacgacct attttgattg
      301 atgaaaaaag aatactaatc ggatttaatg aagatgagat ccggtcattt gtccctcgtg
      361 aaattcgatg tgttgccaga aatctgatct atttacaaaa caataacatc ttaaattcat
      421 agggagggag ccttcgtgga agtgaaagtc tatggatcag cgcactgtgt aacaagtctg
      481 aaggcgaaac aatggttaga acaccatcaa ttgccttatc aatttgttga cttagaagaa
      541 cgcgatctaa gtacagaaga agcagaagaa ttatgcagtt ttgaagaagt gcaagcagaa
      601 cagctatttg cgacttggtc tgaggctttt cacaacattg cgattgacct ttcctgtgta
      661 ggaaaaaaac aactgatttt tttatgcaca caacatacca atctcctcag acgtcctttg
      721 attattatcg atgaagttct atttattgga tacaatgagc agttgatgga acagcttatc
      781 ttaaataaat agactttaac tgttttaatc aagtcactga gcatttcata cagttgtaac
      841 agtcgaacac ttagcatcaa gcgtgttatg tgttcttttt ttgactttat ttttctacta
      901 tagagacatg ctcaatttat gtggtcaaaa ttcaaatcta ggtggggata tagagttaaa
      961 aatatattga catgtgtaaa tttacatgat atattcattt ctgttgtttt aagtagcaaa
     1021 gcaataaaat agtattttaa aaaacatata aaatagttgt tgacatgtta gaaatgacat
     1081 gataagataa ctgagttgtt aaaaaagaac cacttgtgaa gcttgattct atcgaaaaaa
     1141 acttcaaaaa agttcttgac aaataacaaa tgataagtta tgataaatga gttgctgtaa
     1201 gatttcgaaa aacgaaatca aaaaaacttt taaaaaagag ttgacaatta cttgtctctc
     1261 tgatatgata taaaagttgc tacggcagcc aaaaacgaat cggggaacac tcctgatgaa
     1321 gtagaccttt gaaaactgaa caaagtaaga caaaccaaat gtgtagggcg tcttgattca
     1381 attcaagaca acaaaca
//
LOCUS       DAIGOK010000229         1389 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.236, whole genome shotgun sequence.
ACCESSION   DAIGOK010000229 DAIGOK010000000
VERSION     DAIGOK010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.236"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            278..820
                     /locus_tag="NOA01_002703"
     CDS             278..820
                     /locus_tag="NOA01_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633830.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            976..1326
                     /locus_tag="NOA01_002704"
                     /pseudo
     CDS             976..1326
                     /locus_tag="NOA01_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002414636.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
BASE COUNT          551 a          164 c          265 g          409 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcaaat tgatgaagca gatcccaaag ggctgcttca tcaatttttt
      121 cgtaagtgtc cgtacactta ctgtgcttgc tcatatatta atcgatcata tactaatcgt
      181 gtaagaacaa ctttaagcat aatacttata ttgttatacc atagtttgga aaaataaaaa
      241 attatatttc atcaacgttt cataaaaggt gtgtagaatg ttattgaaag tagctataaa
      301 atgctattgt gtaaaaaata aaggaggaat aatttatgaa aaaatagtac caagaggagc
      361 atgcctatta cttgcattat gtttgagctt aagcgcagtt gggtgtcaaa aaagtaatga
      421 aaacaataaa ataaaagaag gacaatccat aagttctgaa gaggaaggca tgtcaattaa
      481 tgcaaatggc aaaaatgaaa cttttaagcc atcagattat acattggagg caaagaaaga
      541 atatgtttat gaatatttag ggcttaaatt taaactctca gacaaattta gaaattatat
      601 agctgataaa aagatagcga tgttagatga tcaaagtcct atagataaag aactaaagta
      661 tgctattttg acatttgaga aaatgacaga agaacaaaag aatgcagtta tagaaaagat
      721 gggcgatgga tataaaaatt ggcaaaatga gcttgaaaga attgggacta ttggtatatt
      781 tgaaaaaaat acatcagaag aaaaaaatct aaaattataa aatgtgatac tcatactaaa
      841 ataggagttt caagtgatgg aaaatacgat tgctatttaa gcacaaatag cggagctgaa
      901 agtaatcttc tggatgaatt aaaaagaact gagattcaaa ttatagataa aaaagaacgt
      961 cctaaaaatg gttttgtgct atcagaaaaa actgatttag aaaatacaga agcatttaac
     1021 aaagaatcgg ttaaagattt acgtaaacta ccaacaaagg acataaatgg caaggatttt
     1081 acaagtaaag attttgagaa gtatgatctt actatggtaa atgtatttgc aacttggtgt
     1141 acagcttgtg taaaggaaat tcctgatttg gttgaggttc agaatgaaat gaaaagcaag
     1201 ggtgtaaata tcgttggagt tgttacagat cctgttgatg ataacggtga aaacaaggaa
     1261 gcaattgaaa agtcaaagtt aatacatgaa aagacaaagg cttcatatcc tttcttaatg
     1321 ccagatggaa tggcaacact ttttttgaca aaaattataa ggtgttgtga atgctgcggt
     1381 gttatggct
//
LOCUS       DAIGOK010000230         1340 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.239, whole genome shotgun sequence.
ACCESSION   DAIGOK010000230 DAIGOK010000000
VERSION     DAIGOK010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.239"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            553..>1340
                     /locus_tag="NOA01_002705"
     CDS             553..>1340
                     /locus_tag="NOA01_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HCI0633831.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNI"
BASE COUNT          472 a          252 c          233 g          383 t
ORIGIN      
        1 acactcctct taatatacag tcctattgta taaagtgtta ctatatacat agtaactgta
       61 tacataaaaa aagtcaagta ttagaaaaaa gttcaaaaga tcaagtgtgc gcttcgctcc
      121 cactcccctc gacgttttag tagcctttcc ctcacttcgt tcagtccaag ccaactaaaa
      181 agttttcggg atacattctc cttctcttcc taataatcaa ttaaaatttt aatctttata
      241 tttctgctaa tcactcagga aacagtacag aaaaacaaac acgtatcata gatataaatg
      301 taatggcata gtgcgggttt tattttcagc ctgtatcata gctaaacaaa tcgagttgtg
      361 tgtccgtttt tgggcgttct gctagcttgt ttaaagtctc ttgaatgaat gtatgttcta
      421 agtcaaaaaa ctctgacagc gcctttatat agataaagtc cgtataattg tgtaaaagta
      481 aaaaggccat ataacagtcc ttttacggta caatgttttt aacgacaaaa acatacccag
      541 gaggactttt acatgaccca agtacatttt acactgaaaa gcgaagagat tcaaagcatt
      601 attgaatatt ctgtaaagga tgacgtttct aaaaatattt taacaacggt atttaatcaa
      661 ctaatggaaa atcaacgaac agaatatatt caagcaaaag aatatgaacg aacagaaaac
      721 cgacaaagtc aacgaaatgg ctattatgag cgcagcttta cgacacgtgt aggcacgcta
      781 gaattaaaag tacccagaac acgtgatggc catttttcac ccacagtgtt tgaacgttat
      841 caacgaaacg aaaaagccct catggcttca atgttggaaa tgtatgtatc aggcgtttca
      901 actcgtaaag tatcaaaaat tgtggaagaa ctttgtggta aatccgtctc taagtccttc
      961 gtttctagct taacagaaca gctagaacct atggttaacg agtggcagaa tcgtttatta
     1021 tcagaaaaaa attatcctta cttaatgacc gatgtactct atataaaagt acgagaagaa
     1081 aatcgagtac tctcaaaaag ctgtcatata gcgattggaa taaccaaaga tggcgaccgt
     1141 gaaattatcg gcttcatgat tcaaagtggc gaaagcgaag agacctggac aacatttttt
     1201 gaatacctaa aagaacgcgg tttacaaggt acggaactcg ttatttctga tgcgcacaaa
     1261 ggattagtct ctgccattag aaaatccttc accaacgtaa gttggcaaag atgccaagtt
     1321 cacttcctaa gaaatatctt
//
LOCUS       DAIGOK010000231         1269 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.241, whole genome shotgun sequence.
ACCESSION   DAIGOK010000231 DAIGOK010000000
VERSION     DAIGOK010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.241"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(45..1193)
                     /locus_tag="NOA01_002706"
     CDS             complement(45..1193)
                     /locus_tag="NOA01_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCI0633832.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSS
                     GKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQ
                     RKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDI
                     ICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICS
                     ECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          370 a          226 c          194 g          479 t
ORIGIN      
        1 taccaagcta tccccgtagt tcctacggtt cttgatgatt ggatctaagc caatcccata
       61 gaatgtagtc ttagaccttc ggtcagaata ttgatactag cattgatatc acgatcatga
      121 tgagtatgac aaacaggaca agtccattct cgaatttcaa gagatttctt gccatcttta
      181 tgtccgcact ctgaacaaat ctgactagac ggaaaccatt tatctacttt gatgattttt
      241 cgtccatacc agtcagcctt gtattgtaat ttagtcacaa actttgacca agaaacatca
      301 gaaatacttt tagctaattt atgatttcgc aacatacctt ttgtgtttaa gtcttcaata
      361 cagataatat cgtgattttt gatcatttct gtactcaact tattcagaaa atcagtacgt
      421 tgattcatta ctttttcatg caatctagct actttgcgtt tttgtttttg ataattttta
      481 gcttcaaata gattggtacc tttattttta gccaataaag cacgcctaga caatttgcgt
      541 tgttcacgct ttagcttctt ttccattttg gacgtgaatt tattattatc aattttttgt
      601 ccatcagaaa gaatcgcaaa gtccataata cctaagtcaa taccaattgt ggaattagtt
      661 ttaggtaatt cgataatttc ttctttacac aacaaagaaa tatagtattt accgctagaa
      721 tgacgtgata ttgtagcaga tttgataatc ccttttggtt gtctatgaag cttaattctt
      781 actaatgact tcaatttagg aactttgatg aatttactat caatcaaagc aactgtacca
      841 ttttgattat tcgttgtata actctgaaca ggatttttct tacttttgaa acgtggaaat
      901 ccaacggatt tatcccgaaa aaaattcttg tatgctttat ctaaattaag ttgggcattg
      961 gctaaagcaa ggctatcaat ttctttcaaa aacggaaatt ctttcttgta tttagctggt
     1021 gtcggaaaag tcattttttt agaagaatca tttttaactt cttcatacgc cttttttcgg
     1081 tcatcaagca ttagattgta gaccttacgg acacaaccaa aagatttggc aaagaagatt
     1141 tcttgttcct ctgttggata gattctgaat ttgtatgctt ttagttgttc cataaagttc
     1201 acctactttc tatttatgat tgtttccttg attttggata tacttcttaa tcacatcaat
     1261 tggtgcgcc
//
LOCUS       DAIGOK010000232         1245 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.242, whole genome shotgun sequence.
ACCESSION   DAIGOK010000232 DAIGOK010000000
VERSION     DAIGOK010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1245
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.242"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            13..>1245
                     /locus_tag="NOA01_002707"
     CDS             13..>1245
                     /locus_tag="NOA01_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HCI0633833.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGF"
BASE COUNT          414 a          247 c          242 g          342 t
ORIGIN      
        1 acgatacgct atatgaatga ttctatcaaa aaaatgctga gaataacaga gaaagatttg
       61 atgattacag aggtctctta cgagaccctt cagaagaaaa agacgttggt cgtcgatgct
      121 gttctctcgc ctactcctcg tgcttgtaga agttgtggtt ctactgtggt agatggaaac
      181 gggaaagcaa ttatagtgaa aaatggaaaa aaggaaacga ttgtccgttt tgaacaatac
      241 aatcatatgc ctttggttat gcgcctaaaa aagcaacgct atacctgtaa aaactgtcgc
      301 acccattgga cggctcaaag ttattttgtc caacccagac attcaatcgc aaatcatgtt
      361 agatataaaa ttgcttcttt actgactgaa aaagtatctt tatcttttat tgcgaaaagc
      421 tgtcaggtat ctttgaccac cgttattcgt acattgaaag agtttaaaag ctatttacca
      481 aagcaatcta agaagatcct ccccagagta ttgatggttg acgaatttcg ttcgcatgct
      541 tccatagaag ataagatgag ctttatttgc gcggatggcg aaacaggaaa attaatagat
      601 gttttgccta cgcgtaaatt acctcgatta acaagctatt tcttaggttg taccaatcca
      661 gaagaagtag aattcttggt gacagacatg aacgccgcct acttccagct caccaaacgt
      721 gttctgccaa atgcgaaagt cgtgattgat cggtttcata ttgtcaaaca catgaatcaa
      781 gcgttcaatg agttgcgtat ccgtgaaatg aatgaacttc gtaaagccgg acagaaaagc
      841 caggcagaaa aactgaaaaa gaactggcgc tttttgctaa aaaatcgtgc aaacatcaac
      901 cattatgaat acaaaacatg gaaaagtttc cgagcaccaa aatacccatt tcttactgaa
      961 gcaatgatga ttgatcgatt gcttgagttt tctccgcccc taaaggaggc gtatcccttt
     1021 tttcatgaat tagttgaagc ctttcgagac aaagaccctg acttattttt ctccttattg
     1081 gcagaacttc ccgaaacgtt agatgacagc tttcgggaaa agcttcaaaa ccttctgacc
     1141 tatgaagaag gcatcaccaa cgcaatgatc tatccttatt ccaatggaaa aatagaagcg
     1201 aagaataccc acataaagac aatgaaacga gtatcctacg gattt
//
LOCUS       DAIGOK010000233         1184 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.243, whole genome shotgun sequence.
ACCESSION   DAIGOK010000233 DAIGOK010000000
VERSION     DAIGOK010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.243"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            58..333
                     /locus_tag="NOA01_002708"
     CDS             58..333
                     /locus_tag="NOA01_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633834.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            760..>1184
                     /locus_tag="NOA01_002709"
     CDS             760..>1184
                     /locus_tag="NOA01_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HCI0633835.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTD"
BASE COUNT          447 a          205 c          178 g          354 t
ORIGIN      
        1 catataacca aaggcgctat gatttccata tgaaaaaaat aaagtcaggt gaaaaaaatg
       61 aaacttgagg ataaaaaagt cattttgaag aaaatcaaac gactaccaga ccaaatagat
      121 ggctatacga tctgttcttt caaaaaagac aggagtataa aagcttatac gcaaaacaca
      181 gaaaaaggaa taatctattt cctacatgaa gaaggttacg atacccaaac ctttatgttt
      241 actgaaaaaa aagaatttca taaacagatg aaaaaactga ttgaaaaaga atttccaaga
      301 agtcataaac tctatgtgaa tcagcaattt taacttctaa ctgttcttta ttaattaaaa
      361 atgttctgta ataaaataaa actttataac aactataaca aaaccgaatg caaaacagaa
      421 gttctataag ttataatcat agttgttagg tgattcatct agtaaaaaca ccagatttca
      481 aatttcacac aatttaaact agatcaatca tcaaccttct tcaaattgtt agaacccttt
      541 cccgaggagc tgcagcataa gcaaaaaaac aaacaggaag ccaaatggct aagacatttt
      601 ttctattagt ctagttgttt ctcgtcgcaa gtttaagact ttagaaagtt atttctaatg
      661 atgtttgggt tgttttgatc aatgcttatg cacagctcct tacctattat ttatctcaac
      721 agatctaaca aattaatgga ctatttaagg agttttttta tgcttacgat ctattcttct
      781 cgcacacata gttaccatct gatggcaaac tggctgaaag accatcaaat gccttacaga
      841 gaacgaattt tcacacctaa acagccttta acaaggaaag agatatttta tttattgtca
      901 ttaacagaaa acggatttga cgatctatta gctaaacgct cgattgatta taaaaatttg
      961 aaagaccaaa tcgaccaatg tacgacctca gagcttgtcg atctgatcgt ctcgcatttt
     1021 actttgttac aacgtcccat cattactgac ggaaagaaat tattagtcgg attcaatgaa
     1081 gatagcatgc gtctttttat ccctgtacaa tggcgcagaa gtcaactgaa atctgtctat
     1141 aacgatttta catgggtaag caatctagaa aattctacgg attt
//
LOCUS       DAIGOK010000234         1151 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.244, whole genome shotgun sequence.
ACCESSION   DAIGOK010000234 DAIGOK010000000
VERSION     DAIGOK010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.244"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            8..268
                     /locus_tag="NOA01_002710"
     CDS             8..268
                     /locus_tag="NOA01_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633836.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(630..869)
                     /locus_tag="NOA01_002711"
     CDS             complement(630..869)
                     /locus_tag="NOA01_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633837.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
BASE COUNT          405 a          163 c          213 g          370 t
ORIGIN      
        1 atgaaatatg aataacgaaa ctatcatttt aggaaaaaaa tatatttgcc aacctattgg
       61 cttgaaacaa aaagtggttg gagaagtaat cagtaaatta gaaaattgtg ttgtgatctg
      121 cgtagacgaa tatcatgacg tcgatcgcaa tgaaatatta gaaaaatgcg gaaaagtagt
      181 ggcaaaatat actcacgttt atgagttgga aacaacagaa tatctatata actatagcaa
      241 caaatatgaa catactttag tttcataaag atttaaataa agtgccttca aaagcgagaa
      301 gaccaagctt ttgaaggcac tttatttagt tggcagtgat tgttcatgat ttttgattta
      361 ttcaaaaggt tcgcctagag aatctgtctc tgtaacgagg cctggtggag aaactcgata
      421 aaaacctata ctgccagaac tgtcctattt tcggatagac aaactagtag ttttaaaaag
      481 atcatccgat ccaatcattt caaaaaatgc aaaactgata tcaggatcat ttagctcagg
      541 cttgttatcg taaagcattt gtaaaggttg catttagtta ggagtaaacg cgaaactatc
      601 atcattattt gctgaagaag atgaagaagc taattcagag tttgtttgaa ataaaggaga
      661 cgtgctgaca gtttctttac cctgtttttt gcagaggtag aaacttttat atcggaagtt
      721 tctaccggag aatttttctc atttgtagaa attgttgaat cgatactatt cgaaatttgc
      781 gtatctgaag gtatgttctt tgatgcagaa gtcccacaag cagtaagtaa caataaattt
      841 gataaactga taaacagaat tcttttcatt atgtacacac tccttatagt atcgtttagc
      901 atacacctat gctcagtatt aggcaaaatc aatgcaaaac tatcaagtct tttatagtat
      961 ctattagtct tagtaataca tatatggaga gggtgatatc ccagttcaaa ccagatacat
     1021 tggttaccat ctgtattaac gattatgcct ctaaacgctt tttttatgaa aaaatttata
     1081 agtgaaaacg taggtatgat attatctaga ggtaattttt ttgggtattt aaatagttgc
     1141 taggaggata g
//
LOCUS       DAIGOK010000235         1070 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.245, whole genome shotgun sequence.
ACCESSION   DAIGOK010000235 DAIGOK010000000
VERSION     DAIGOK010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1070
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.245"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(<1..864)
                     /locus_tag="NOA01_002712"
     CDS             complement(<1..864)
                     /locus_tag="NOA01_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCI0633838.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFL"
BASE COUNT          333 a          132 c          182 g          423 t
ORIGIN      
        1 taaaaaacca atctcctgtt tttgtcgaaa agagatttga tttggaaaaa aacaatagaa
       61 aaaataatta aagtaggctc tctctgtttc tgtctcttca caagggcgac tcactgaaaa
      121 agaagtaaag tattctgaat aatcatgtgt atgctgcaat aagtccatca attcagtgat
      181 ttcagaagaa gtttttggaa aatatgcctg ttgtttgttt cgtaatatga ataaagttaa
      241 cagcaagtag atattttttt cttgttttga aattttttct atttgctcat ctgtactgtt
      301 catttttagt gcatttaatt caagtgagga aaatggccaa tcgttacctc caaatgtatc
      361 tgaaagaaaa aagtaaagat agagacggat agataactca tttccttcaa gagtacagca
      421 attttttaca gataggatcc gtaaatggaa tttttttaag gaatgattga gctgttttgt
      481 cagtttttcg agataggagg gcgatatata aagttgttca agtaaatatt tttttggtac
      541 agcagtttgt ggataattga ttagtaacat caaatagtta aaacgattgg attcttttag
      601 gtagcataat tttaacttag aaaaaacagc taacttttta gtttgatcgg tttctttggt
      661 ttggatctct cctttatgat tagaataaat aaattcatca atcaataaaa attgcttgag
      721 tgatacattt atttttcgga tcagttcttt gattgtccgt tttggaatat gtaagtcatt
      781 ggataattct tgtaaagaac aattattttt ttgaatgacc atttgtagaa caagtaactc
      841 tttttttctt gcttcaatta gcataaatac ctccttttta ttctgttttt gtatatagtt
      901 agacaaaaac tgtgagtgga aagtcatttc agtctgtgtt attctcacag agtgtcgcct
      961 tcttttttat ataacgttat ttaatttttt tgttaccttg tttattctat tgtttatttc
     1021 ctagggtatt ttgtcaagat attcttgttt ttaaggcgga aaaattgatt
//
LOCUS       DAIGOK010000236         1068 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.246, whole genome shotgun sequence.
ACCESSION   DAIGOK010000236 DAIGOK010000000
VERSION     DAIGOK010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.246"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..>1068
                     /locus_tag="NOA01_002713"
     rRNA            <1..>1068
                     /locus_tag="NOA01_002713"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          281 a          249 c          324 g          214 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggc
//
LOCUS       DAIGOK010000237         1064 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.247, whole genome shotgun sequence.
ACCESSION   DAIGOK010000237 DAIGOK010000000
VERSION     DAIGOK010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1064
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.247"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(39..995)
                     /locus_tag="NOA01_002714"
     CDS             complement(39..995)
                     /locus_tag="NOA01_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633839.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          381 t
ORIGIN      
        1 ttttagaaaa agcagtcgga ctttctaaaa gaagttcact attcgatcct gtcgatgatt
       61 tcttcaggaa aatcgtctat ttgcttgtca attttgctgt ctttgtattt ctcttcttgg
      121 atagtcaaca aaatctttgt aagattcccc cagtctactg ctcttggcgg atcgagccaa
      181 accactactt gagatgcact ttcttcatat agattattcc tattcgtgat tataacatcc
      241 gggaactcag gctttcctgc ctctatcact tccgtgttca gattaatgta caagcttaaa
      301 tttttcttga taaattgatt ataggcagca ccataagaaa aatcgatgat gatctttact
      361 ggttcagaaa tcagttgaat gggtatgtga ttgatcaata ataataaata actgaaaaaa
      421 agaaaaaatt tatcttgaag caactctttg cgttttgacg ttagatcatt tatatactct
      481 atcaaatagc aataaaatgc tggatagttt tctctaaaaa aagaaacatc aagtggaaat
      541 tcatcagctt tattaatagt gtaatgcaaa agtttaaaat gtattttggt taactctctt
      601 cggatcaatt ggctcgtctc ttctcgcaac agattaaact gattttcaaa actttcaatg
      661 aatcgttcat tcaaccattc gattgtttca tcttcatttt ttctcaagta tttttgatcc
      721 aatttatcat gaagataaag ccaagagagt gtcttattga taaaaataac ggatttttct
      781 cctaatactc cttttaagtg gtaaaatcct atatccgaaa aaaatagcga ctgattgcta
      841 gcaatatcta aagaatcagc ttgttcagcc aacaggttga ttgctaaaaa gtggaataat
      901 ttcttttttt cataagcaga cagaggataa tactgtttta aatcttttaa cattttttct
      961 gtttttataa ttataggtag cgtatacaat tccacaaaac ttatctctgt tgagttaaaa
     1021 agattatata aaaaaagccg aatgttttta ggattccctg tcaa
//
LOCUS       DAIGOK010000238         1050 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.248, whole genome shotgun sequence.
ACCESSION   DAIGOK010000238 DAIGOK010000000
VERSION     DAIGOK010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.248"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            89..1024
                     /locus_tag="NOA01_002715"
     CDS             89..1024
                     /locus_tag="NOA01_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCI0633840.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYTGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT          366 a          167 c          238 g          279 t
ORIGIN      
        1 gttataaaaa tgaacattgc atagtagaga ttctttgatt atactaattc aaggttatat
       61 gaaaaaagag aaatgagatg gatgatttat gaaactttgg atgaaaatcc ttttaggcgt
      121 cgctttagct ctgattgttg cgttagtgtt tgcagggaat tatttttata attatgctgt
      181 tgtgccttct gagaaagact ttttagacga agatacacct ggtacaacac agacatccaa
      241 agaaacggat gcacataaat ggtttagttc aaaagaaaac cgaagcgatt ggaccatcac
      301 ttcaaaagac ggtctgaaat tatcagcgat ttatcttcct gctgaaacga aaagtgaaaa
      361 aacagctctt gtggctcatg gctatatggg aaacgctgaa acgatgacca attacgcaaa
      421 gatgtttcat gatatgggtt acaatgtcct tgttcctgat gcaagaggac atggaaaaag
      481 cgaaggggat tacactggat ttggatggcc agagcgtaaa gactatgtac agtggataaa
      541 caaagtgcta gaaaacaatg gaaaatcgca agaaatcgtt ctatacggtg tcagcatggg
      601 agcagcaaca gttatgatga ctagcggaga aaaactacca gataatgtga aagcaatcat
      661 tgaagattgc ggttattctt ccgttcatga tgagcttgct tatcagctag acgatatgtt
      721 tagtcttcca gcatttccgc ttatgcaagt aacgagtcta gtgacaaaag tccgtgctgg
      781 ctactttttc ggtgaagcaa atgcagtgga acaattgaag aaaaaccagc gcccaatgct
      841 ctttatacat ggagacgaag atacatttgt accatttgaa atgttagata aggtctatcg
      901 tgcaacgaag ggaccaaaag aaaagtatgt tgtaaaagga gccgagcatg cggaagcata
      961 caagacagat cctgaaaaat atcagcaagt agtccaacaa tttttaagcc aatatgttca
     1021 ttgaacgagt ttaaaagtag gggatgaaaa
//
LOCUS       DAIGOK010000239         1035 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.249, whole genome shotgun sequence.
ACCESSION   DAIGOK010000239 DAIGOK010000000
VERSION     DAIGOK010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1035
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.249"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(<1..>1035)
                     /locus_tag="NOA01_002716"
     rRNA            complement(<1..>1035)
                     /locus_tag="NOA01_002716"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          218 a          324 c          234 g          259 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg ccttt
//
LOCUS       DAIGOK010000240          991 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.250, whole genome shotgun sequence.
ACCESSION   DAIGOK010000240 DAIGOK010000000
VERSION     DAIGOK010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.250"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(207..401)
                     /locus_tag="NOA01_002717"
     CDS             complement(207..401)
                     /locus_tag="NOA01_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633841.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(501..587)
                     /locus_tag="NOA01_002718"
                     /pseudo
     CDS             complement(501..587)
                     /locus_tag="NOA01_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          364 a          157 c          128 g          342 t
ORIGIN      
        1 tattgaaagt aactctatgt agcaaaaaat atctagaatt tcatttcctc aaaactgatc
       61 gatattgtat ctaaaaatat atactaccta aaataggtag tatatattaa aatatgtgct
      121 tttgttagaa aaatcaatac ataaattatc atatctgtat atgtcattta ggtatttcag
      181 ttatgttata atagttttgc tgtctgctac cccggcctaa tctggcaagg ggaggaggag
      241 atataatgaa ataccttttt gattttgttt ttgcaatcaa gccacttgca gatgtagtgt
      301 gcagttacct ctgcaaatgg cttgattgca aaattgccaa aaaagacagt aagcctaccc
      361 gttaattcta taatatagaa acgtaaaaaa aaccccatca gcctgcacgc tgatggggtt
      421 tggtgatata atgaaatacc tctttgttac tcataattat accataatta gtcacgaatg
      481 caaccaatag aaaaaactac ttagaacggt agatcatcgc taataacaat gcattgtcca
      541 ccaaatggat tttgtgaagc attaaataca ttgttattaa ttgtcgctgc ttttaactag
      601 gcctctcccc ttctattata aaagaagata tatatcattt cttaattacc tactacctta
      661 aaccaaaatt taagtactac ctaatttaag tagtacttaa acaatctaaa aaatagttat
      721 cccaattgtt atgcttttat aatacgatta tttgtaacaa aatgaatatt atataattaa
      781 acactaccat atttaggtag tgtttaatta ttagcaaaac aaaaaatatt tattctattc
      841 tcttaacttc aactaagtta tgctgcacac ctttatataa atcatatgca actcattagc
      901 tagacaaact acaagcatgt agttttatct gttaattatg tactacctaa ataaagtagt
      961 ttataattag caagtatata tatttaaaaa t
//
LOCUS       DAIGOK010000241          982 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.251, whole genome shotgun sequence.
ACCESSION   DAIGOK010000241 DAIGOK010000000
VERSION     DAIGOK010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.251"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..>982
                     /locus_tag="NOA01_002719"
     CDS             <1..>982
                     /locus_tag="NOA01_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HCI0633842.1"
                     /translation="LDISWFFQGIEEFSKVVIRNTVIKLSSVLLIILFVSTKDDLWIY
                     VFITSITNFLGSLVFWLSIHGKVGKPLFNSNVFKQLWKPAFIILTPQLFMQVYTTLDK
                     TIVGYFVDPTALSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKSENILVYTKK
                     SFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVSPIGGVFANQ
                     FAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLVFFFRVFFVR
                     KEIKISYFFGKNVVLFFIVNLTLTTSAYYFLPQLFSSSFLDMAIKSIMIFI"
BASE COUNT          297 a          133 c          162 g          390 t
ORIGIN      
        1 attagatatt tcttggtttt ttcaaggaat tgaagaattt tcgaaagtgg ttattagaaa
       61 tacagttatc aaattatcat cggtattgtt aataatcctt tttgtttcaa ctaaagatga
      121 tttgtggatt tatgtgttca ttacttcaat tactaacttt ttaggaagtt tagttttctg
      181 gttgagtatc cacggaaaag taggtaaacc actctttaac tctaatgttt ttaaacagtt
      241 gtggaaacca gcttttatta ttttgactcc tcagttgttt atgcaagtgt acacaacttt
      301 agacaaaacg atagttgggt attttgtgga tccaacagca ttatcttatt atgatcagtc
      361 tcaaaaaatt gctcgtatta tattggcagt cttaacatca attactattg ttatgttgcc
      421 taaaatatct aaagcacagg cagagggaaa atcagaaaat attttagttt acacaaaaaa
      481 atcttttgat tacacgatta ttattgcatt aattcttttt agtgtggtgt ttatcaatac
      541 aaaagaattt gttccatggt tttttgggga gaatttcaaa ccgatgacaa taaacatgct
      601 tatttcttgt ctaattatag ttgttagtcc aattggtgga gtttttgcta accaatttgc
      661 aattgctata gaaaaagata aggagtatgc ttatccaatg atcattggtg cgattgtaag
      721 tattattgga aatgtagctt ttgtgcctac ttatggcgct gtagcggcta caatcgtact
      781 cgtggtagta gaattattag tctttttctt tagagtattt tttgtacgaa aagaaataaa
      841 aatatcctat ttctttggga agaacgttgt cttatttttt atagtcaatc ttactttgac
      901 tacttcggcc tattactttc ttccacaatt attctcttct tcatttttag atatggcaat
      961 taaatcaata atgattttca tt
//
LOCUS       DAIGOK010000242          957 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.252, whole genome shotgun sequence.
ACCESSION   DAIGOK010000242 DAIGOK010000000
VERSION     DAIGOK010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..957
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.252"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            773..>957
                     /locus_tag="NOA01_002720"
     CDS             773..>957
                     /locus_tag="NOA01_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCI0633843.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINV"
BASE COUNT          351 a          156 c          118 g          332 t
ORIGIN      
        1 attcggaatt aaaaaccacg ataggtaact ttaaacaaac atgaagagac tttaataaga
       61 cttctttatc tgaaaacact tttgtttctc tctttccttt ctacatcatt aaatagtatt
      121 ttatacgtta tctaaaatac tattcctctg tcaaaataag ctaacttact tcattataaa
      181 atatccactt atcgctttca tgtatattcc accgtaaaaa gtacatttta tcaccgagaa
      241 ctcatcattt ttcatcatga tgaatactat ttacatatat ctagttcaaa gacaataatt
      301 attatgatcc gttattatct aagcactata cagataatag gaatttttcg tttctaaata
      361 ttccaatttg ggaatccaat aaaaattttc gttcaaatac gtgtctattc gaactctttt
      421 ctagttgtta attatgaagg gggaagaaat aaaaaataca atctatacac gaataagaat
      481 attatccaaa aggaggtttt agaaaagatt tattatacgt aaaaagctca agacattttc
      541 ttagccataa ggacatgttt gatatttaag aagatatatg aattttcttt tctaagtata
      601 aagataacta tccattatca actaaaaaaa catttttgaa aataaacatc agcagtccta
      661 tcaatgcatg tgagcttata gaagttattc gcagcgcagc ataattgcat tagcttcttt
      721 tcgtgttttt tgcctctttt ccctttaaat cttaatggag gaggtgtgca atatgtcaga
      781 taacgacgaa cgcgtaaatg cccagttgat tcgattcttt gaaaaggtca ttttgaacac
      841 cgctaatact tattataaaa aacaagaaga aattcaaaaa catgaacaac ttgatcaact
      901 cccaccatat ttgtttacta aagatactat aaaatttaaa caaccgatta atgtttt
//
LOCUS       DAIGOK010000243          943 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.254, whole genome shotgun sequence.
ACCESSION   DAIGOK010000243 DAIGOK010000000
VERSION     DAIGOK010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..943
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.254"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..>943
                     /locus_tag="NOA01_002721"
     rRNA            <1..>943
                     /locus_tag="NOA01_002721"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          293 a          187 c          274 g          189 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact agg
//
LOCUS       DAIGOK010000244          928 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.255, whole genome shotgun sequence.
ACCESSION   DAIGOK010000244 DAIGOK010000000
VERSION     DAIGOK010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.255"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..351
                     /locus_tag="NOA01_002722"
     CDS             <1..351
                     /locus_tag="NOA01_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCI0633844.1"
                     /translation="REAVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGF
                     EDAFQYTVQGNSHNRLKSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHD
                     EWIYSSRKYINFDK"
     gene            408..800
                     /locus_tag="NOA01_002723"
     CDS             408..800
                     /locus_tag="NOA01_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCI0633845.1"
                     /translation="MEEVLIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
BASE COUNT          356 a          106 c          164 g          302 t
ORIGIN      
        1 agagaagctg ttaaaggaat ttttaagttc acagatatta acttagcgcg tgaggctaaa
       61 aatcgattga ttcatgatta tatcgatcaa ccaaaatatt caaaagcttg cgcatcattg
      121 gatgatggat tcgaagacgc ctttcaatat accgtacaag gaaattccca caatcgacta
      181 aagagtacca atctaattga acgactgaat caagaagtac gcagaagaga aaagattatt
      241 cgcatcttcc ccaatcaaac atcagccaat cgcttaattg gagccgttct tatggaccta
      301 catgatgaat ggatttattc ttcaagaaaa tacatcaatt ttgataagta gaaatggtaa
      361 aaacattgta tagcatttta cacaggagtc tggacttgac tgagtttatg gaagaagttt
      421 taattgatga taatatggtt tttgatattg ataatttaaa aggatttctt aatgatacca
      481 gttcatttgg gtttatagct aaagaaaata ataaaattat aggatttgca tattgctata
      541 cacttttaag acctgatgga aaaacaatgt tttatttaca ctcaatagga atgttaccta
      601 actatcaaga caaaggttat ggttcaaaat tattatcttt tattaaggaa tattctaaag
      661 agattggttg ttctgaaatg tttttaataa ctgataaagg taatcctaga gcttgccatg
      721 tatatgaaaa attaggtggt aaaaatgatt ataaagatga aatagtatat gtatatgatt
      781 atgaaaaagg tgataaataa atgaatatag ttgaaaatga aatatgtata agaactttaa
      841 tagatgatga ttttcctttg atgttaaaat ggttaactga tgaaagagta ttagaatttt
      901 atgatggtag agataaaaaa tatacatt
//
LOCUS       DAIGOK010000245          928 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.256, whole genome shotgun sequence.
ACCESSION   DAIGOK010000245 DAIGOK010000000
VERSION     DAIGOK010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.256"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            55..928
                     /locus_tag="NOA01_002724"
                     /pseudo
     CDS             55..928
                     /locus_tag="NOA01_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          362 a          153 c          178 g          235 t
ORIGIN      
        1 gacagaaaaa ttaaggtgtg tagactagaa gaaaacatac caggaggaat ttttatgtct
       61 aagagaacac gaagaacttt ttcacaagaa ttcaagcaac aaatcgtcaa tctttactta
      121 gctggaaagc cacgtgtaga aatcattcga gaatatgaac taacggcttc agcatttgac
      181 aaatgggtaa agcaatctaa aacgagtggt tcattcaaag aaaaagataa tcttacgcct
      241 gaacaaaaag aattgttaga actacgtaaa agaaaccagc aattagaaat ggaaaatgat
      301 attttaaagc aagcagcgct gatattcgga cgaagagaca agtaatcgat gcgaataagc
      361 atctttaccc tatatcagcg atgtgcagaa tattaggtct atcacgtcag tcctattatt
      421 atcaatcaaa accaaagaaa gacgaatcag aacttgaaga agtagtcgct gaagaattta
      481 tccgcagccg aaaggcctac ggctcaagaa aaataaaaaa agccttatca aaacgaggca
      541 ttcagatcag ccgacgaaaa attagtagaa tcatgaaaaa tagaggatta aaatcgagct
      601 atactgttgc ttattttaaa gtacatcatt ctacttgcaa tgaagccaaa acgacaaacg
      661 tattgaatcg taaattctta agagacaacc cattagaagc gatcgtaaca gacttgactt
      721 atgtacgagt cgggaaaaaa tggaattatg tctgtttcat tttggatctg ttcaatcgag
      781 aaattctcgg ctattcttgt ggagaacata aagatgccgt tctagtaaaa aaagcattta
      841 gccgtatcaa acaacctctg acagaggttg agatttttca tactgatcgt ggaaaagagt
      901 ttgataacca agctattgat gaattatt
//
LOCUS       DAIGOK010000246          927 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.257, whole genome shotgun sequence.
ACCESSION   DAIGOK010000246 DAIGOK010000000
VERSION     DAIGOK010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.257"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            5..685
                     /locus_tag="NOA01_002725"
     CDS             5..685
                     /locus_tag="NOA01_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCI0633846.1"
                     /translation="MIINAGVIIGLMLVIFSGKQYADNILLWGGEDNIEKIHNNLTIL
                     DTALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKTNLEN
                     QLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQSTINSL
                     NQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTANKAQQ
                     IVNEHTIQ"
BASE COUNT          412 a          111 c          136 g          268 t
ORIGIN      
        1 aaagattatt attaatgctg gagtaattat aggattgatg ttagtaattt tctccggaaa
       61 gcaatatgct gataatattt tattatgggg aggggaagat aatattgaaa agatccacaa
      121 caatttaact atattagaca cagctctaga aaataaagag caaaaaatta gtattttaaa
      181 ttcacaattg tcttcaaaca accaaactct tgaacaacta agaactaata ttgaagtttc
      241 caaacaaaaa gtggcatctt tagaaaacga caagaatcaa ttagtatcag agaaaactaa
      301 tttagaaaat caattgtcag caaaagactc tgaattgcaa ggaaaacaaa atgaaattaa
      361 tgcgaaaata gacgaaatta accaaaaagt agctgaaata aatcgattaa atagcgattg
      421 gaataatcgt ttacaaaatg aaaagaacaa atttaatgaa gaacaatcta ctattaatag
      481 tttaaaccag caaattaatc aaatgttgag tgaaaaaaat aacattcaaa atcaactgca
      541 agctactcag caaaagctag aagaagaaaa gtcaaaaaat agtagtttga ataattacat
      601 tgaaaagctt caaaaagcta aaaatgatgt agaggatacc gcaaataaag cacaacaaat
      661 tgtaaatgaa catacaatcc aatagatata gttttaattt aacaaatggt atgtttggag
      721 ataatgagat agaatataaa tgtcataata ttatacaatc taaacaccgt ttccttttct
      781 atttttgatg acatcagtat atagctactc gaaagagtag tctttttatt attctcgctt
      841 attattgata tgaaaatttc gatttgacaa aaggtaccga ataattttgt aatatgaatt
      901 attatcaaga agtaattatg atgataa
//
LOCUS       DAIGOK010000247          898 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.258, whole genome shotgun sequence.
ACCESSION   DAIGOK010000247 DAIGOK010000000
VERSION     DAIGOK010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.258"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            21..848
                     /locus_tag="NOA01_002726"
     CDS             21..848
                     /locus_tag="NOA01_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCI0633847.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKFLQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
BASE COUNT          349 a          122 c          173 g          254 t
ORIGIN      
        1 ttaaagggaa ggaaattgtt ttgattaaag tagatggagc attactaaaa gaataccgaa
       61 agaaaaaggg gttaacacaa gtacaattag cagaaggcat ttgtgaacaa gcaactgtca
      121 gcaatatcga aaacaagaat atgtcgccag gcatcaatat cttaagtgcg ctttgcaaaa
      181 agctgaatgt cgagatctcc gatatattag tagaaacaga agaagacaaa atcaggaaaa
      241 aaataaatga gattcaatca ttgaattctg tatcaaaata ccaagaatct tataaatttt
      301 tacaaacgat cgatcaggaa aaagtaagta attctgatct tttaaatcaa attttatttt
      361 atcaaggact gcttgatcat ctttatgcga aaaaaccaga tgcagcatta ttttatttta
      421 atcaagtgtt ggaacaaacg accgaaacag atatccatca cttacaagca gctgcaaacg
      481 tggcaatgat ctacttgtca aaaggagaat tagattttgc gaaagtttat gtggagcgta
      541 cacttaaaat attgagtgag actgattttg ataatctgat ggtctgcatt gtatattatg
      601 atatcgccac ctattaccgt aaaatcgagg attatgataa agcaatccaa ttatgtgaga
      661 agggtattga gtataataag aaacataaat cgacttatgc attggaatac ttgctttatg
      721 aaattgcttc ttgccataag caattaggag aagatgatta tttagaaaga tatatggacg
      781 caaaaaaaat agcgcgtttc aacggaaatg attacgcggt aaaggtgatt gaaaacgacc
      841 tgaaataaag tggttgtgaa atcagtgttc tgatttgaat accgtttatt cgaaaaaa
//
LOCUS       DAIGOK010000248          876 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.260, whole genome shotgun sequence.
ACCESSION   DAIGOK010000248 DAIGOK010000000
VERSION     DAIGOK010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 876)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..876
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.260"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="NOA01_002727"
     CDS             complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="NOA01_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HCI0633848.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          251 a          186 c          134 g          305 t
ORIGIN      
        1 gtacacggtg ttccaaaagt agcttcttcg gaaataagtt ggaatttcac aaaaattcaa
       61 gaagtaattt tcgtaaattc cttcttattt cttgaagctg attacttctg tcacaacctc
      121 tttttattat tgagaatttg gtcgaggaat aatcgttttt gccatccaaa tatccggttt
      181 gtcccagcca tttgcatttg gtaagacacc tacgatttta taacctaatt tttcatagaa
      241 ttcatacgga tgttcacgaa ggttctggat ggaagccact ttatcaaatg tatgctcata
      301 caggtccgtt tgacttaacg ttgttccatg gtctaaatca tccgtaccta aataaatcgt
      361 gattcctcct ctggaagcta cttctttttc taagtaattg actaatcgag tacctatttg
      421 gttctttcgt cgggagcttt ctacaactaa tggatgcaat tcccaacctg tgataccgta
      481 ttgagggatt gcaccaataa atcctactaa ctcatcttgg tctaccgctg ctaccgcgat
      541 tcgttctgga ttcatcattt cttctacttc ttctgccgag ctgtctccat attcttccgg
      601 ccaagtcagt ctcagtaaat cagaaagctg atctttcaat actggattat tacggtcaaa
      661 ttcactgatt atcatacgtc ttcccccttg ttcttgctta ttctaacagt ccttttgaaa
      721 aaacgaaatg ttctcgctca aaaatcttta gctaccaatc agtatctcta ttctttcatt
      781 atttatctta cttataaaat aaaaaaatta tacagaaaca ctttattcaa aataggaaag
      841 atcaggaaag ccatcaattg gaggaaaaaa ctctgt
//
LOCUS       DAIGOK010000249          872 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.261, whole genome shotgun sequence.
ACCESSION   DAIGOK010000249 DAIGOK010000000
VERSION     DAIGOK010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.261"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            5..>872
                     /gene="bsh"
                     /locus_tag="NOA01_002728"
     CDS             5..>872
                     /gene="bsh"
                     /locus_tag="NOA01_002728"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HCI0633849.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGETKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTY"
BASE COUNT          294 a          117 c          159 g          302 t
ORIGIN      
        1 aactatgtgt acgtctatta cttatgtaac aagtgatcat tattttggaa ggaattttga
       61 ttatgaaata tcttacaatg aagtagttac tgttactcca agaaattata agttgaattt
      121 tcgaaaggta aatgatttgg atactcatta tgcaatgatt ggtattgccg ctggtatagc
      181 tgactaccct ctttattacg atgcgacaaa tgaaaaagga ttgagtatgg ctgggctaaa
      241 tttttctggg tatgctgatt ataaagaaat acaagaaggg aaagacaatg tatctccttt
      301 tgaatttatt ccttggattt taggacaatg ctcaacagta ggagaaacta aaaaattgtt
      361 aaaaaatatc aatttagcaa atataaatta tagtgatgaa cttcctttat cccctttaca
      421 ttggctatta gctgataaag aaaaatcaat tgtcattgaa agtatgaaag atggacttca
      481 tatatatgat aaccctgtgg gcgttcttac caataatcct tcatttgact atcaattatt
      541 taatttaaac aattatcgtg tcttatcgag tgaaactcct aaaaataatt tttcaaatca
      601 aataagtttg aatgcctata gccgcggtat gggagggata ggcttgcctg gagatttatc
      661 ctcagtatct cgttttgtta aagcgacttt tacgaagctg aattctgtat ctggagattc
      721 agagtcagaa agtattagtc aatttttcca tatcttaggt tcagtagaac aacaaaaagg
      781 tttgtgtgat gttggtgatg gaaaatatga atatacaatt tattcttctt gttgcaatgt
      841 tgacaaagga atctattatt atcgaacata tg
//
LOCUS       DAIGOK010000250          868 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.262, whole genome shotgun sequence.
ACCESSION   DAIGOK010000250 DAIGOK010000000
VERSION     DAIGOK010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.262"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            65..>868
                     /locus_tag="NOA01_002729"
     CDS             65..>868
                     /locus_tag="NOA01_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCI0633850.1"
                     /translation="MHKISVIVPAYNVENYLENCLESISNQTHSNIEIIVVDDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILVFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYT"
BASE COUNT          335 a          108 c          159 g          266 t
ORIGIN      
        1 aaattgttac tggtaatgat acatttgtaa aaataaaaaa gatgattttt aggaagtgaa
       61 gagagtgcat aaaattagtg ttatagtacc cgcatataac gtagaaaact atttagaaaa
      121 ctgcttagaa tctatttcca atcagacaca ttcaaatatt gaaataatag tagtcgatga
      181 tggttccccg gatcgaagtg gggaaattgc agaagaatat aaaaagaaag atatgcgatt
      241 aaaagtgata catcaagtga atggtggttt aagtgcggct aggaattctg gtcttgattc
      301 tgcaacagga gattttgtgt gctttattga cagcgatgat tggatagaac cagactatct
      361 tgagttacta tattttggta cgcaaactta tggagcagat atctcggtgc taaaaataaa
      421 aaagatttca agtttagaac aactaaaaga acatacaatt gctgagagaa aatgggaaat
      481 atttgataga gaacaagcaa tgaaaaactt atttataaat aataaaattg gctattctgc
      541 aaataataaa ttattcaata gaaatttatt tgataatata agattcccac tgggtaaatt
      601 aatggaagat aaggctacta cttatttgtt gatcgatcat tccgaaaaag ttgctgtcaa
      661 ttcatctgct aaatatcact attaccaaag ccctaaaagt atactgagag gaagcttcaa
      721 tcctagaatt tttgattctt ttgaagtcca tgaagatata ctagtattca tagataagtt
      781 ttatccggaa ttatcaatta atgttagaac tcgctatgtc tatgaagcaa taaggatgat
      841 gatgacaatg attaaaaata actatacc
//
LOCUS       DAIGOK010000251          797 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.263, whole genome shotgun sequence.
ACCESSION   DAIGOK010000251 DAIGOK010000000
VERSION     DAIGOK010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.263"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            100..630
                     /locus_tag="NOA01_002730"
     CDS             100..630
                     /locus_tag="NOA01_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633851.1"
                     /translation="MISDNKWEINLKRFISEYIYTIETKSSFSLNEDSDPLKKYTIQC
                     KKNKDEKLFVIRPDLMKSNTHYIKEKPKDCDYIIISEANKTVFLIELKSSAQTSPAKE
                     ISEQIDGGYKWLQHLSFLTKYDFTEFSYKIIKVCCKFEARQDRKNLFKPNEYGIYNMN
                     GPKLDLRELYRKCNDL"
     gene            complement(625..>797)
                     /locus_tag="NOA01_002731"
     CDS             complement(625..>797)
                     /locus_tag="NOA01_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCI0633852.1"
                     /translation="KGRPKLYSADTKNPQRRLVYENIVKDLNEGVSISKIAKNYNVTR
                     QTIYRIKKDLNL"
BASE COUNT          312 a           96 c          123 g          266 t
ORIGIN      
        1 aatttctaac atcagttatt atgataattt tgatgatgtc aattatgatt tacaggaaag
       61 atataatgat ttaatcgaag aattagagga gagaggagaa tgatttcaga taataaatgg
      121 gaaataaatc tcaaaagatt tatttcagaa tatatatata cgattgaaac taaatctagt
      181 ttttctctta atgaagacag tgatcctcta aaaaaataca ctattcaatg taagaaaaat
      241 aaagatgaaa aattgttcgt aataagacca gatcttatga agagtaatac ccactatata
      301 aaagaaaaac ctaaagattg tgactacatc atcataagtg aagcaaataa aacagtattt
      361 ctgatagaac ttaaaagctc agctcagact tcccctgcaa aagaaattag tgaacaaatt
      421 gatggtggat ataagtggtt acaacatctt agttttctaa caaaatatga ttttactgaa
      481 ttcagttata aaattattaa ggtatgttgt aaatttgagg caagacaaga tcgtaaaaat
      541 ctttttaaac ctaatgaata tggtatttat aatatgaatg gtccaaaatt agacttaaga
      601 gagttgtata gaaagtgtaa cgatctataa attcaaatct tttttaattc tatatattgt
      661 ttgtctagta acgttataat tctttgcaat ctttgaaata gagacgccct cattcaagtc
      721 cttaacgatg ttttcataaa ctaaacgtcg ttgaggattt tttgtatctg cactatagag
      781 ttttggacga cctttat
//
LOCUS       DAIGOK010000252          796 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.264, whole genome shotgun sequence.
ACCESSION   DAIGOK010000252 DAIGOK010000000
VERSION     DAIGOK010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.264"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            27..257
                     /locus_tag="NOA01_002732"
     CDS             27..257
                     /locus_tag="NOA01_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HCI0633853.1"
                     /translation="MGFRTKRSLSSKKNWVYSQTIFYGGIVLISLLSSTLYSLNIIDV
                     STSNSISIIGIIIAAIITQLFLVFGEKKRSKK"
     gene            complement(348..713)
                     /locus_tag="NOA01_002733"
     CDS             complement(348..713)
                     /locus_tag="NOA01_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633854.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
BASE COUNT          297 a          104 c          138 g          257 t
ORIGIN      
        1 tttttgatag gagtagaaat tcaagtatag gttttagaac taaacgaagt ttgtcttcta
       61 aaaaaaattg ggtatactca caaactattt tttatggagg aattgtttta attagtttac
      121 tttcttcaac actttattct ttgaatatta tagatgtttc cacctcaaat tcaatatcta
      181 ttattggaat cattattgct gcaattatta cacaactgtt tttagtgttt ggagagaaga
      241 aaagaagtaa aaaatagaaa aactagtgat agttttggtg atataagaaa aacatatatg
      301 tataaaaaga agaaagtagc ttctctttgt acatatatgg tttgttttta tggatgccga
      361 gcttttttat aagataaggc taccgcccat aacgacccaa ggatttgtgg aactaagtaa
      421 atacctgcaa gaagcaagat cagctgggtc ggttttaaag gatgatagaa gtaacgccat
      481 tcagtaaaat tggcgcaaaa taaataggaa gtgacagtag atataatatt ggcaatgaat
      541 aaagatataa tacgcctgtt cctttgaaaa taatatccag caaaaagaga taaaaatatc
      601 aatgtgatga acccgcttac ggaatgataa cgaaaatcaa gcagtaaggc gaaatgcaca
      661 taaggaacaa aagataaaag caacggaagg tttttccagc aagtttttat catctttcat
      721 caccacctaa caagtgttat tattttgtta gttttattgt aaattgaatc ctgcaataat
      781 caattacttt ttcatt
//
LOCUS       DAIGOK010000253          792 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.265, whole genome shotgun sequence.
ACCESSION   DAIGOK010000253 DAIGOK010000000
VERSION     DAIGOK010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.265"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(62..262)
                     /locus_tag="NOA01_002734"
     CDS             complement(62..262)
                     /locus_tag="NOA01_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCI0633855.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT          256 a          133 c          122 g          281 t
ORIGIN      
        1 aattttttcg tacaaaaaaa ccgtcttttc tttcgaaaag acggttaaaa gtcaattttt
       61 attaagcttt aacaatgttt gttgcttgag ggccacgttg accatcttca acatcgtaag
      121 acactgcttg gccttcttct aaagttttga agccgtcacc ttggatagct gaaaaatgtg
      181 caaatacatc atttccatct tcaccagtga taaagccaaa acctttgtct gcgttgaacc
      241 attttactgt accgttattc ataaataaat tcctcctgat acgtatcaat gtacgttttt
      301 tcgttgcaat aaatatttga taggcaaaag gagttttatc tatgtagata tatcgctcaa
      361 attacgtttc aaaatagatt actcatttaa tatatcatgc tttttctaat aacacaagga
      421 taatgcaatg aaatataaaa ttcattgtct tctattgtct tcgaatcgaa gaaaaagttg
      481 aataaccgcg attgattcac tttttgtttt cttgtttatt tgcttttatc tgcaactttt
      541 gactttaaaa tttcttaact atgaaaacct ttcaattggt ggtatactta taaatggaat
      601 ttcgttatca acgcgttcgt tgcctttcag caagtattta aagaaaggag aatggctatg
      661 acattctata acaaagatat acaatatcga gtaaccttgg atacagattt gaatctattt
      721 gtcgtttttg acaaagaaaa taccaatcat gtagcgacag gtgttacgat cgaacaagca
      781 gtgcaagaat ta
//
LOCUS       DAIGOK010000254          790 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.266, whole genome shotgun sequence.
ACCESSION   DAIGOK010000254 DAIGOK010000000
VERSION     DAIGOK010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.266"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            123..>790
                     /locus_tag="NOA01_002735"
     CDS             123..>790
                     /locus_tag="NOA01_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCI0633856.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEV"
BASE COUNT          285 a          124 c          143 g          238 t
ORIGIN      
        1 ttttacactc tcataaaaga tagcgtaaaa tattttgtgt aaatagaaat ttagggtaga
       61 aaaaaaagtc cattctgtaa aattaaagtt accacacaga aattttatag gaggactttc
      121 tcatgactaa atttactata gaaattatgc aaacactact caataaaggg gatttagatg
      181 aattatttca ggatcattta gagcgagcga ttaattcact tctacaagca gagctaactg
      241 cttttttaga ttacgaaaaa tatgatcgaa atggattcaa ttcaggaaat tcccgtaacg
      301 ggaattattc acgtacattt aaaacagaat atggcgagtt gaatttaact attcctagag
      361 atcggaatgg cgaattttct caacaaacac taccagcata taagcgaacc aatgattcat
      421 tagaaacgac cattatccaa ttatttcaaa aaggcattac tatggctgaa atctccaaat
      481 taattgaaaa aatgtatggt caccactata cgccacaaac aatctccaac atgagtaaat
      541 tggtggctga agatgtttta gcttttaaag aaagaacgtt agaagccaat tattccgtta
      601 tatttatgga tgcgacgcat atccctgtga agcgacagac tgtttcgaag gaagcagtgt
      661 atattacgat tggtattcgt ttggatggaa ccaaagaagt tcttgggttt actattgccc
      721 caactgagtc tgcttatatt tggaaagaag ttcttcaaga tcttagaaaa cgtgggttag
      781 aagaagtttt
//
LOCUS       DAIGOK010000255          788 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.267, whole genome shotgun sequence.
ACCESSION   DAIGOK010000255 DAIGOK010000000
VERSION     DAIGOK010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.267"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..507
                     /locus_tag="NOA01_002736"
     CDS             <1..507
                     /locus_tag="NOA01_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCI0633857.1"
                     /translation="VLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRG
                     RKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIR
                     RTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEI
                     QDTLESMI"
BASE COUNT          276 a          131 c          116 g          265 t
ORIGIN      
        1 gttttattag tagtgacaga cggattaagc tgtattgaag aaagtatcca tagtgtgtat
       61 ccgaatgccc aatttcaaca atgttgtgtg catgtatcta gaaatatcgc tcataaagtt
      121 cgtgttcgag gtcgaaaaga aatttgtgag gatttcaaat tggtttacca agcgaattca
      181 aaagaagagg cattggatca catcgacttt atgactagga aatggaaaaa gcagtatcca
      241 agagtcgtca atttactctt gaatcctgcc ctattaacct tttataattt ccctcacgcc
      301 atcagacgaa caatttattc gacgaacctg attgaaggct ttaataagca gctaaaacga
      361 tatactcgaa gaaaagaaca attccctaat gaagaatctc tagagagatt cttcatttct
      421 caatttaatc aatataacca aaaattttta ggtagaattc acaagggatt taaagaaatt
      481 caggatacat tagagtcgat gatttaactg taattaacag aatggatttt ccatttacat
      541 ataattcttg acgctaccat aatctaaata accatcatat tcatctacta cttatataat
      601 tgagttttat taaacttttt aagtttctat tctatcaaat atttaaacaa tctctgcatc
      661 cgaaaagact acctcgtata gttttactaa caatattttt attagcgtgt tcaaatcctg
      721 atttcttgta tcaattattt tctgttcact ttcaataaga aaaaagtctt tacttccatg
      781 gataataa
//
LOCUS       DAIGOK010000256          734 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.268, whole genome shotgun sequence.
ACCESSION   DAIGOK010000256 DAIGOK010000000
VERSION     DAIGOK010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.268"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            20..>734
                     /locus_tag="NOA01_002737"
     CDS             20..>734
                     /locus_tag="NOA01_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCI0633858.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLSVDDKVHPLKLSWSIDRITIVG
                     KLKENIYYHTPNDVLILDFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENIAYIEILKW
                     QEGKGRIDFNPNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFITQYRVV
                     DPVSFKPIYGRNGKLETAYWGSRSSERQIRMYNKKLEQEKKRKIVPKEIVSWWRLEMQ
                     LRRGKATDWHAMVRESLDSF"
BASE COUNT          251 a          106 c          164 g          213 t
ORIGIN      
        1 ctttgaggag gaactaaatt tgagtaaaaa aggcacgatc ataaatgagt gggaaaaatt
       61 aacagccccc tctaacaggc ggttaagtgt tgacgataag gtacaccccc taaaattaag
      121 ttggtcaatc gatcgaatta caatcgtcgg aaaactgaaa gaaaatattt attatcacac
      181 tccaaatgat gtgttgattt tagattttga acagcttatg cgcctaaatg aaggtaacgg
      241 ttatttaaga tctgttagta ataatggttg gcagttgtta gaccaacatg aagagaatat
      301 tgcttatatt gaaattttaa aatggcaaga ggggaaaggg cgtatagact tcaatccgaa
      361 taagattagt caattcttag ctagttccat gaaaaatttt attcatgatt tgtttatcga
      421 acctcacttt tcacgtgcag atattgcttg cgacattatc gatgtacctg atgagtttat
      481 tacacaatat cgggtagttg atccagtttc ttttaagcct atttatggac ggaatggaaa
      541 attagagacg gcatattggg gatctcgctc ttctgaacgt caaattcgga tgtataataa
      601 aaaactggaa caagagaaga aaagaaaaat tgtgccaaaa gaaatcgtga gttggtggcg
      661 gttagaaatg cagctaagac gtggcaaagc aacagattgg catgcgatgg ttcgggaaag
      721 tttagatagc tttg
//
LOCUS       DAIGOK010000257          733 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.269, whole genome shotgun sequence.
ACCESSION   DAIGOK010000257 DAIGOK010000000
VERSION     DAIGOK010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.269"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            25..>733
                     /locus_tag="NOA01_002738"
     CDS             25..>733
                     /locus_tag="NOA01_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCI0633859.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISN"
BASE COUNT          259 a          126 c          139 g          209 t
ORIGIN      
        1 aagaattata aggagtgagt acaaatgacc tatacccatc ttacaacgga tgaacttgta
       61 ataatagagt cttatttcaa aatgaatcaa tctgttgcta aaactgccca ttgcttgaat
      121 cgttcaagac aaacgatcca taaagtatat ctgttcttca agcaaggaaa atcagcctta
      181 gaatattatc aacagtataa gaaaaacaaa tcaaactgtg gtagacgtcc gcttgtttta
      241 cccgaggaac aatcagaata tattcaaaga aaggttgttc aaggatggac acccgatgtg
      301 attgttggtc gtgcagcgtt tcctattagt tgttctgctc gtaccattta tcgtatgttc
      361 aaaaaggggc tatttgattc ttctgactta ccgatgaaag gcaagcgtaa accgaatgga
      421 catcaagaaa gacgtggaaa acaaactttc cgccgctcta ttcatgaacg tgaaaaggat
      481 tatagccaat tctcaaatga gtttggtcac cttgaaggtg acactatcgt aggtctgaaa
      541 cataaaagtg ctgtaattac cttagttgaa cgattatcaa aagttatcat cacattgaaa
      601 ccgtgtggta gacaagcgat tgatattgaa aaaaaattaa atcaatggtt tgaatctgta
      661 ccgaaaaacc tattcaaatc catcactttt gattgtggaa aggaattttc aaattggaaa
      721 cagatcagta atg
//
LOCUS       DAIGOK010000258          733 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.270, whole genome shotgun sequence.
ACCESSION   DAIGOK010000258 DAIGOK010000000
VERSION     DAIGOK010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.270"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(<1..>733)
                     /locus_tag="NOA01_002739"
     CDS             complement(<1..>733)
                     /locus_tag="NOA01_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCI0633860.1"
                     /translation="HIRASLKDAVEEGLIPFDPTRKAVIKGKDSLKPKEDKYLDYDQF
                     KSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDIDFEDQIITINKTWNYK
                     LNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEVKNPHNFVFFNLKNGLV
                     SSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNILSVSKRLGHSSLETTM
                     STYLHIVRELEDQDKEKINAVFDSL"
BASE COUNT          221 a          122 c          104 g          286 t
ORIGIN      
        1 tataaactat cgaacacagc attgattttt tctttatcct gatcttcaag ctctcgaaca
       61 atatgaagat aagtagacat tgtagtttct aaactgctat gtcctaaacg tttcgatacg
      121 ctaagtatat ttactccttg ataaagtaaa atagatgcat gtgtatgcct tagtccatgc
      181 aaagtaaatt gcttttcaat ccctaattct tttaattttt tgcgcaaata tttgctgacg
      241 gcgtttgatg aaactaatcc attttttaag ttgaaaaaaa caaaattatg agggttttta
      301 acttcaaaat tctcgaataa ttctttttga ttgattttaa acttttttaa gagatcaatc
      361 gtatgtttat caatggaaat tttcctattt gaagtttcgt tttttgtttt tccccattca
      421 tttaatttat aattccatgt tttattaata gtgatgattt gatcttcgaa atcgatatct
      481 tcccatgtta atcctagaag ttcagcaaat cgcattccag tagcaccagc aactaacacg
      541 agcataggag aagagtactg cgcagaaagg ttttcttcta cgagtttcat taaagattta
      601 aattgatcat aatctaaata tttatcttct tttggcttca atgaatcttt tcctttgatt
      661 actgcttttc tagttggatc aaacggaatt aatccttctt ctacggcatc tttcaacgat
      721 gctctaatat ggt
//
LOCUS       DAIGOK010000259          728 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.271, whole genome shotgun sequence.
ACCESSION   DAIGOK010000259 DAIGOK010000000
VERSION     DAIGOK010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.271"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(25..98)
                     /locus_tag="NOA01_002740"
     tRNA            complement(25..98)
                     /locus_tag="NOA01_002740"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(62..64),aa:Ile,seq:cat)
BASE COUNT          258 a          113 c          124 g          233 t
ORIGIN      
        1 gaacgattca attggctaca ttaatgtacc ctgtaggact cgaacctacg accggacggt
       61 tatgagccgt ctgctctgac caactgagct aagggtagtg gttgttgcca cataaagcca
      121 taaacaatca accagtagaa tgcgtggcaa caaacctgtt atcgcatatc ttggagtgtg
      181 actatttatg agtgatagtg aagatatgcg tctccatata tgtatggttg agacagataa
      241 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
      301 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
      361 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
      421 tacactcttt ttgaggtgaa cttttatgct gatggaagga aaaacacaat tatggtttaa
      481 gcatgttcct tcaaataagt tttttaatta taaactctag agaaattaat tcatcctaac
      541 ataaaataat tgttcactca aaagtttttc tagaacaatt aaaacagcag gcttcacatt
      601 cgctatacat ttgtaagaag taataattcc tgtactaaaa ggcataattg ctttaattat
      661 tttttgactt ctcttcaaac acaagaagta gttgtgtaat aattgcagaa ataaggattc
      721 caataata
//
LOCUS       DAIGOK010000260          697 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.272, whole genome shotgun sequence.
ACCESSION   DAIGOK010000260 DAIGOK010000000
VERSION     DAIGOK010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.272"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(54..629)
                     /locus_tag="NOA01_002741"
     CDS             complement(54..629)
                     /locus_tag="NOA01_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCI0633861.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          230 a          151 c          125 g          191 t
ORIGIN      
        1 aatgaaaaaa cagagccaga ttagcattat tttgctaatc cagctctgtt tattcagcct
       61 atttttcttt ttgaatatat caagcgttgg ggcaacatag tgatgactaa tgaaacaacg
      121 acgatagaca acaagcgatc aaggtagtct gtacccgcct gtaccaagat cacacttgtt
      181 gtctgactta aacctaaccc atgtaatagc tgaacgatca tactggatcc tgaggaggtg
      241 atcccgccaa ataactgaac ggtaatgatc gaagaaacga tcgttccagg taccgacaaa
      301 atcaaagcga gtaataacag tttcaagctg tttttatctt tgccgagata tcttggcaaa
      361 atcaatccag caagcaagcc tgtgagaatt tggattggcg aataatacag agaaaaaata
      421 tctgtagtca ctccacttaa aataccgctg gacaatcctg tcagcattcc aggtacaggc
      481 ccaagcaatg caccagcaaa aatcgttccg atagaatcga gatagattgg caaacgaagc
      541 cataaagcaa tcgttcctcc aatataattg atcccaatac acaaagccat cactgtaagt
      601 acgcgagttg ccattttgtt tttattcatt ttgttagccc atctttaacc tttccaaatc
      661 tttcataatc gtttcttctt gtgcatttaa aagaacg
//
LOCUS       DAIGOK010000261          681 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.273, whole genome shotgun sequence.
ACCESSION   DAIGOK010000261 DAIGOK010000000
VERSION     DAIGOK010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..681
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.273"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(71..472)
                     /locus_tag="NOA01_002742"
     CDS             complement(71..472)
                     /locus_tag="NOA01_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCI0633862.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
BASE COUNT          176 a          151 c          111 g          243 t
ORIGIN      
        1 tttgtcaaaa aaaaccagac aaaaagcggt cctttcacgt ttttagtctg gttcgatttc
       61 ctttattctt ctaaacaatc gttacttcta catcgatatt tcctctagtt gctttggagt
      121 aagggcagaa ttcatgcgca tcttctgcaa ttttttgggc ttcttcttcg ctgatgccat
      181 cgatatgcac ttctaatact acaccgatct gatagccgcc atcatctaaa ttataaagag
      241 agacacgtgc ttttactgtg gactctgctg tcattcctgc ttctttgatc atatgttgta
      301 ccgccccatt gaaacaagaa gcataacctg ctgcaaataa ttgttctgga tttgttcctt
      361 ctgcatggat tcctggagga gtgattttca tatgcatcga gccatttggt gcctcgactt
      421 ctccattgcg cccgccatgg ttgatcattg ctgtttcgta tgcttttttc attgtactgc
      481 tcctttcgat tgaccatttg ctacttggct ataattatag tgtatgacaa gtaaggcgct
      541 ttctacaaaa attctgctta ttcgaatacc agaatgccgt cttctactat ttttccaaaa
      601 aacaaaaaaa tactactcgt gaacaacagc gtcatatttg ttccaaaata aatagcaatc
      661 ttttctacat aatcttttat a
//
LOCUS       DAIGOK010000262          679 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.274, whole genome shotgun sequence.
ACCESSION   DAIGOK010000262 DAIGOK010000000
VERSION     DAIGOK010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.274"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
BASE COUNT          241 a          113 c           80 g          245 t
ORIGIN      
        1 caaattactt catcaaaaca ttgatataat ttttattata tcagtattta ttcttgatac
       61 ataaatgaga aaataaatac tttaaatcta ttgactacaa gtagtcatta agtcttcata
      121 agatgtacaa tatgacatta aaaaaataaa agcttacgac catccgttaa atctgacact
      181 aaataatgga ctgctttctt ttgtattagc aggttacgtt gactatgaat attcaccaga
      241 ttatttggag aaacctcttc aacacgagaa caatgatatt aattttagat ttgtctacga
      301 gtcaaaaagt aaaacatggt tagttgatca attaagcacg ataaacacaa gcttccatga
      361 attacctaaa cctgaaaatg aagttgagat ggtcattaaa atcttctaaa aaagaagcgt
      421 tgaaggggaa atattccttt caacgctctt ttttatatgc ttagaaacat aaataaattg
      481 tatactttct ttttagcccc tattactttt cacgaaaaat atcttttttt cgacgctagc
      541 gtttctctac ttctaatact gtacacagtt tttgtattat tgaaaaatta ctcattattt
      601 agcattaatc cacatcgttt tatcaatcga ccttcatact aaactacttt aaagtcatct
      661 tccttctcgt ttttcatta
//
LOCUS       DAIGOK010000263          660 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.275, whole genome shotgun sequence.
ACCESSION   DAIGOK010000263 DAIGOK010000000
VERSION     DAIGOK010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.275"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            66..>660
                     /locus_tag="NOA01_002743"
     CDS             66..>660
                     /locus_tag="NOA01_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCI0633863.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDE"
BASE COUNT          213 a          114 c          154 g          179 t
ORIGIN      
        1 ttcattgttt taggcatact agtaatagat agttcttcat tgaaaataga aaaagaggtg
       61 gcaccatgat tgaatttcaa aatgtgtcaa aaatttataa aggtggaaaa gtcgctgtag
      121 aggacgtcaa tctttcattt gaaaagggtg agttcatttg ttttatcggt acgagcggaa
      181 gcggaaaaac gacgacgatg cggatgatca accgaatgac tgatccaaca caagggaaaa
      241 ttttgattga tggaaaatcc atccaagaaa ttgatccagt ggaattgcga cgtcagatcg
      301 gctatgttat ccaaagtatt ggattgatgc ctcacatgac gatccgtgaa aatatcactc
      361 ttgtccaaaa attgttgaaa gtcagtcaag aagaacggaa taaaacagct gaaaaaatga
      421 ttgatcttgt ggaattgccc cgtgagatgt tagatcgcta cccacatgaa ttatctggtg
      481 gtcagcagca gcgtattgga gtcgttcgtg cccttgcagc gaatcaagat atcatcttga
      541 tggacgagcc attcggtgcg cttgatccaa tcactcgtga ttccttgcaa gaccttgtca
      601 aagatttaca agagcgtttg ggaaaaacga tcgtatttgt aacccatgac atggatgaag
//
LOCUS       DAIGOK010000264          650 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.276, whole genome shotgun sequence.
ACCESSION   DAIGOK010000264 DAIGOK010000000
VERSION     DAIGOK010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..650
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.276"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(132..509)
                     /locus_tag="NOA01_002744"
     CDS             complement(132..509)
                     /locus_tag="NOA01_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633864.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
BASE COUNT          202 a          113 c          113 g          222 t
ORIGIN      
        1 caaaaaagaa gcagatccgt tagcggttct gcttctttta gtttattata ttttcttatc
       61 aataatagga acctgatttt aaaagcggct acctctttac cgtgcctctt tacatacaaa
      121 aaatagagga attaagcgta aatggcttgg ctattataac taaaatgctg gttatggaga
      181 tgttgcagca agttttcgag aaaagcgtat tccatctctt gtccattgaa taagatctgg
      241 atacgataat gatttttgaa acgcttctgt tcatgaatct gactgattga aagttcgcct
      301 ttgtgttctg tctgataaag aaaagtcctt ttacggcatt cagcacagcg cttggttgct
      361 cggcggattt ttcggaatgt ataaaaacta gtatctttat tgcataaata aacgtctttg
      421 atcgacagca aatcattata tagacaggaa cgttcttcag ctgttaattc tgataaaaaa
      481 agctgacctt gttttattgc atcttccatc ggcaagtgcc tcctcgaacc cattatccaa
      541 atttttagtt aattctaata taccatcatt aagtataaat gtaaacgctt gttgatggta
      601 taagattttc gtttctgata aaacacgaac attaaaagaa tctaatgcct
//
LOCUS       DAIGOK010000265          637 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.277, whole genome shotgun sequence.
ACCESSION   DAIGOK010000265 DAIGOK010000000
VERSION     DAIGOK010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..637
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.277"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            51..>637
                     /locus_tag="NOA01_002745"
     CDS             51..>637
                     /locus_tag="NOA01_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HCI0633865.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKWHYLYRAIDADGLTLDI
                     WLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKLKEYGFYQGTEHRTIK
                     YLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEA"
BASE COUNT          231 a          106 c          126 g          174 t
ORIGIN      
        1 tactatcaaa taaggtagaa taatagaaaa agatagcagg aggaatgacg atgaatcatt
       61 ttaaaggaaa gcaatttcag caggatgtga ttattgtagc cgtgggctac tatcttcgtt
      121 ataaccttag ctatcgtgaa gttcaagaaa tcttatatga tcgtggcatt aacgtttctc
      181 atacgacgat ttatcgttgg gtgcaagaat atggcaaact actctatcaa atttggaaaa
      241 agaaaaataa aaaatccttt tattcatgga aaatggatga aacgtacatc aaaattaaag
      301 gaaaatggca ttatttgtat cgagccatcg atgcagatgg tttaaccttg gatatttggt
      361 tacgtaaaaa acgggacaca caagcagcct atgcttttct taagcggtta gtgaagcagt
      421 ttgatgaacc gaaggttgta gtcacagata aagccccctc tattacaagt gcctttaaga
      481 aactaaaaga atacggcttt tatcaaggga cagaacatcg taccattaaa tacctgaata
      541 atttgattga acaagaccat cgtccagtaa agagacgcaa taaattctat cgaagtttac
      601 gcactgcctc taccacgatt aaaggcatgg aagccat
//
LOCUS       DAIGOK010000266          603 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.278, whole genome shotgun sequence.
ACCESSION   DAIGOK010000266 DAIGOK010000000
VERSION     DAIGOK010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.278"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            1..603
                     /locus_tag="NOA01_002746"
                     /pseudo
     CDS             1..603
                     /locus_tag="NOA01_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
BASE COUNT          201 a          107 c          115 g          180 t
ORIGIN      
        1 ccaacatgac taaagtgctg actgaagaag taaatgcatt taaagttaga actctaaatg
       61 ataagtatgt cgctattttc atggatgcta cttatattcc tttaaaacgg caaaccgtct
      121 ccaaagaagc aatctatatt gccattggta tacgagaaga cggcactaaa gaagtactaa
      181 gttatgcgat tgctccgact gaatcgacct acgtttggaa tgagttgtta caggacattt
      241 actccagagg ggttcatgat gtcttactgt ttattaccga tggcttaaaa ggcatgaaag
      301 atacgattca ccagatttat cctaaagcta aataccaaca ttgctgtgtt cacgtttccc
      361 gtaatattgc tcataaagta cgtgtcaaag atcgaaaaga aatctgtgat gattttaagg
      421 ctgtttatca agctagctca aaggaagagg caaatacctt tttagggagt atgattgaga
      481 agtagcaaaa aacttatcct aaagtgacgc agtcactgat aaaaaatcaa gatttattga
      541 ctttttatga gtttccgcct ggtatccgcc gaagcattta ctcaactaat ttaatcgaat
      601 ctt
//
LOCUS       DAIGOK010000267          601 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.279, whole genome shotgun sequence.
ACCESSION   DAIGOK010000267 DAIGOK010000000
VERSION     DAIGOK010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.279"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
BASE COUNT          259 a           70 c           79 g          193 t
ORIGIN      
        1 atatacttta tttcaaccga aaatactatt aaaacaataa ctgaagattt agagaattag
       61 tagtcgattt cgagttatat attgtaaaag aaaaaatcgg ttataaaata actaataaag
      121 catatttttg attctttaca agaaaaacta aaatcatcaa gaatatataa ttatttgtgt
      181 gaatctaatt gaatggaagt tttatgctac ttaactacaa gcaatcttag ctgtttggtc
      241 gttaaaaata acttaaatag gcgaagtcct tcaaaaccaa cagtatttca cataaagatt
      301 gtaactcgtg aaaagtatta cacattaaga taaatactaa tctgtttgct tcaaattgct
      361 gattaaaaag taaagtaatt atagaaggaa atattatata aaggagattt tatcttgata
      421 cataatgaag atagtagagt aaaagtacca gcgctattac atttcaaaag attgggctat
      481 gagtaccaaa ctaaaaaaat caaaaaattg atagtcgaaa taatattttt gtagacattt
      541 ttaccagttc aatcaataaa attaataact caaactttac cgaagagaaa atacaattat
      601 t
//
LOCUS       DAIGOK010000268          599 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.280, whole genome shotgun sequence.
ACCESSION   DAIGOK010000268 DAIGOK010000000
VERSION     DAIGOK010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..599
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.280"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..>599
                     /locus_tag="NOA01_002747"
     CDS             <1..>599
                     /locus_tag="NOA01_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCI0633866.1"
                     /translation="KGKGRFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVRE
                     IRALEKNIKELDKAIEQVIVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKL
                     AKYAGLSWKVNQSGNYQSQNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYK
                     EVPKHQHKRALVLTARKFTRLVDTLLRKHQLYTPPRSVI"
BASE COUNT          223 a          116 c          110 g          150 t
ORIGIN      
        1 aaaggaaaag gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt
       61 accatgagtt accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt
      121 ctcgtacgag aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa
      181 caagtaattg ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa
      241 gtctatgctg ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca
      301 aaattggcaa aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg
      361 caaaatacac ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct
      421 gccaactctg taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag
      481 gaggttccaa agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt
      541 ctggtggata cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgataga
//
LOCUS       DAIGOK010000269          598 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.281, whole genome shotgun sequence.
ACCESSION   DAIGOK010000269 DAIGOK010000000
VERSION     DAIGOK010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.281"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            143..535
                     /locus_tag="NOA01_002748"
     CDS             143..535
                     /locus_tag="NOA01_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633867.1"
                     /translation="MLDDRLTCSNCGTLFYYEEVRNIVKHRKKEMPIRCPNCNEVIKR
                     KLSHGYFVSYELYEESKINLKKKPKYYKNLKTGAIITEKEFKNLVTEEESRFYNLSGN
                     ICPGFMDQIIENESIESIENYVPVDLPK"
BASE COUNT          232 a           57 c           90 g          219 t
ORIGIN      
        1 tgaacgactt gtaattctat attttttctt ttaagtaaaa aatataggtg agtttttttg
       61 acttcatatt tataataaag acacgtaaat caattatttt tcattttact ttaactaata
      121 atgtttattt ggagggatta ttatgttaga tgatagattg acgtgttcta attgtggtac
      181 attattttac tatgaggaag ttagaaatat tgtaaaacat agaaaaaaag aaatgcctat
      241 acgttgtcca aattgcaatg aagttattaa gcgaaagttg tctcatggtt attttgtcag
      301 ctacgaacta tatgaagaaa gtaaaataaa tctgaaaaaa aaaccaaaat actataaaaa
      361 tttgaaaact ggagctatta taactgaaaa agaatttaaa aatttagtga ctgaagaaga
      421 aagcaggttt tataatttgt ctggtaatat ttgccctgga tttatggatc agattattga
      481 aaatgagtca attgaatcaa ttgaaaacta tgttcctgtt gacttaccaa aatagaaaaa
      541 ataatcctgt ttttacagtg aattatttta attttgattt tcaataaaaa tatattac
//
LOCUS       DAIGOK010000270          597 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.282, whole genome shotgun sequence.
ACCESSION   DAIGOK010000270 DAIGOK010000000
VERSION     DAIGOK010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.282"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..290
                     /locus_tag="NOA01_002749"
     CDS             <1..290
                     /locus_tag="NOA01_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCI0633868.1"
                     /translation="ITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPII
                     SVQDSYGLVDVFKNQQEKLLKSSQELLDYIGNYETGASTEKVLELLERDIK"
     gene            287..>597
                     /locus_tag="NOA01_002750"
     CDS             287..>597
                     /locus_tag="NOA01_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HCI0633869.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFLQFLIGLSVSFLYIAY
                     I"
BASE COUNT          204 a           75 c           98 g          220 t
ORIGIN      
        1 taatcacaga ttattcatct atttttttcg attttgcgct aacaaaaaaa ccaattattc
       61 tttttaacta tgatgaaaaa gaatatagct tagaaagagg tttttatata cagttaagtg
      121 agttaccaat tataagcgta caagatagtt atggccttgt tgatgttttt aaaaaccaac
      181 aagaaaagct tttgaaaagt tctcaagagt tacttgatta tattggaaat tatgaaacag
      241 gggcaagtac agaaaaggtt cttgagttgt tagagaggga tataaagtga aaaaaacagt
      301 aaaagatata ttttataatg cgatatatca agtctttttg attgttttgc ccttacttac
      361 tatacctata cttagtcgta gaataggttc aactggttta gggatttatg ggtatgtttt
      421 ctcaatatca caatttttaa tgactgtcat tgctgtggga atgaatcctt ttcgtattcg
      481 aaatatcgct aaatcaagga aagataaaaa agcactatct ttacaattct ggaatattta
      541 ctttcttcaa tttttaatag gcttgagtgt ttcctttctc tatatagcct atattta
//
LOCUS       DAIGOK010000271          592 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.283, whole genome shotgun sequence.
ACCESSION   DAIGOK010000271 DAIGOK010000000
VERSION     DAIGOK010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.283"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..>592
                     /locus_tag="NOA01_002751"
     CDS             <1..>592
                     /locus_tag="NOA01_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCI0633870.1"
                     /translation="LNYRIIWLIKKEAIITDLPDYIKRIDSDSFLAAFYLYTAKYWID
                     DSRKTIKYAKRKKQFYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGN
                     RYSSEKYVSAFNIVPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIED
                     NGITQLEWLGVDNLREFFKKQQQELNLMTKFHPNVHS"
BASE COUNT          227 a           79 c           97 g          189 t
ORIGIN      
        1 attgaattac agaattattt ggttaatcaa aaaagaagcg attataactg atttaccaga
       61 ttatattaaa agaattgatt ccgattcttt tttagctgcc ttttatttgt atacagctaa
      121 gtattggata gacgatagtc ggaaaacgat taagtatgca aaaaggaaaa aacaatttta
      181 ttttcaaaca tggcacggta cgcctttaaa aaaaattgaa tttgatgcaa aagataagtt
      241 acctaaacgt tacctaagct atgcgaaaaa agatagtgag tcaattacat atttattatc
      301 tggtaatcgc tatagttctg agaagtatgt ttcagcattc aatatcgttc cttctaaaat
      361 attagaagta ggaactccta gaaacgatga attagcctct agtaaagagc aagtgtttga
      421 cttgaaaaag aaacaaatca atgtcttatt tgctccaact tttcgaaata atattgagga
      481 caacggaatt acgcaactag aatggttagg tgtggataac ttacgtgaat tttttaaaaa
      541 gcaacaacaa gaattaaatt taatgactaa atttcatcct aatgttcata gt
//
LOCUS       DAIGOK010000272          563 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.284, whole genome shotgun sequence.
ACCESSION   DAIGOK010000272 DAIGOK010000000
VERSION     DAIGOK010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.284"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..530
                     /locus_tag="NOA01_002752"
     CDS             <1..530
                     /locus_tag="NOA01_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCI0633871.1"
                     /translation="RTFRNHRIKETNQLLKSKEQKHYQLGKQLKRYWKLLQKDERKLD
                     YTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQLYQDFLYAVKERDYVSFEELLTN
                     NIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPLECLNNHIKVLKRNAYGFRSFYNF
                     KLRIMIRHGNALIFN"
BASE COUNT          198 a          117 c           97 g          151 t
ORIGIN      
        1 gtcgtacctt tcgcaatcat cgaatcaaag aaacgaacca actattaaaa agcaaagaac
       61 aaaaacacta tcaattaggt aagcaattaa aaagatactg gaaacttctt caaaaagatg
      121 aacgcaagct tgattatact cgacgccttt ggcgacctgg cttcaaagct catttaacag
      181 agaccgatat tgtggatcgc ttactgaaag ggagccctgc tttgcgtgta ggctatcagt
      241 tatatcaaga ctttctttac gctgtaaagg aacgagacta cgtatcgttt gaagaactac
      301 ttaccaataa tatcatgctt cccgaaggct atcagacgac cttgagaacg ttccagaaat
      361 ttctgccaca aatcaaaaat gctttacaac aaagttattc aaatggccca cttgaatgtc
      421 tgaacaacca tatcaaagtg ttaaaacgaa atgcatatgg ctttcgtagc ttttataatt
      481 tcaaattgcg catcatgatt cgtcatggca acgcattgat tttcaactaa aaaagatcaa
      541 gaggaactga tccacttgat ctc
//
LOCUS       DAIGOK010000273          528 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.285, whole genome shotgun sequence.
ACCESSION   DAIGOK010000273 DAIGOK010000000
VERSION     DAIGOK010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.285"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(5..90)
                     /locus_tag="NOA01_002753"
     tRNA            complement(5..90)
                     /locus_tag="NOA01_002753"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="NOA01_002754"
     tRNA            complement(107..178)
                     /locus_tag="NOA01_002754"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="NOA01_002755"
     tRNA            complement(190..262)
                     /locus_tag="NOA01_002755"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="NOA01_002756"
     tRNA            complement(280..361)
                     /locus_tag="NOA01_002756"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="NOA01_002757"
     tRNA            complement(369..441)
                     /locus_tag="NOA01_002757"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="NOA01_002758"
     tRNA            complement(445..517)
                     /locus_tag="NOA01_002758"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DAIGOK010000274          514 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.286, whole genome shotgun sequence.
ACCESSION   DAIGOK010000274 DAIGOK010000000
VERSION     DAIGOK010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..514
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.286"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(<1..132)
                     /locus_tag="NOA01_002759"
     CDS             complement(<1..132)
                     /locus_tag="NOA01_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCI0633872.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          186 a           94 c           92 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactattag acaa
//
LOCUS       DAIGOK010000275          510 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.287, whole genome shotgun sequence.
ACCESSION   DAIGOK010000275 DAIGOK010000000
VERSION     DAIGOK010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.287"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
BASE COUNT          228 a           70 c           82 g          130 t
ORIGIN      
        1 gaattcctta ggcagctttt gctgtgtaga aaggaataaa taataacgaa agttcaaaag
       61 ctaatgccga ctattctaga cattaaagaa gacgaaaacg aacgataaag aaagaaaagt
      121 tatgaataat cgtttattga aaaaaacttg aaaaaataca aaaaattcgt tgaccaagct
      181 aaatttagtt ggtatattat tacttgtcca aaagacagaa gaaaatattt attcaaaaac
      241 ataacaaaaa agttattgac atgttactaa cgacatgata agataactga gtcgttaaaa
      301 aagaaccact tgcgaagctt gattctatcg aaaaaaactt caaaaaagtt cttgacaaat
      361 aacaaacgat aagttatgat aaatgagttg ccgaaagatt tcgaaaaacg aaatcgaaaa
      421 aaacttttaa aaaagagttg acaatcgctt gtctctctga tatgatataa aagttgctac
      481 ggcagccaaa aatgaatcgg gaaacactct
//
LOCUS       DAIGOK010000276          508 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.288, whole genome shotgun sequence.
ACCESSION   DAIGOK010000276 DAIGOK010000000
VERSION     DAIGOK010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.288"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(79..151)
                     /locus_tag="NOA01_002760"
     tRNA            complement(79..151)
                     /locus_tag="NOA01_002760"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(116..118),aa:Lys,seq:ctt)
BASE COUNT          162 a          112 c           83 g          151 t
ORIGIN      
        1 aataaaaaaa gaacagcgta aatgttttcg aaatgctttc gcaccaacat ttacctgttc
       61 cgtgggtttg gcacccaatg ggccgtgagg ggctcgaacc cgcgacccgc tgattaagag
      121 tcagctgctc taccaactga gctaacggcc cgtacaaaaa gtacttaata atagtagcac
      181 taagaatttt aaaatgcaag actacattta aagaaaatca gaaaaatctt tacttcctta
      241 taggaaatca ttcatagatt attcacattt gctcgctatt atataagcat accaacaaac
      301 aaagttcttg caagagaaca aaccctaaca cttttttcat tttttactcc tttcccgccg
      361 tctccccggc gggtctcctg ttcttaacga taaaagacaa gaaggcttag ccttcttgtt
      421 ttttgttttc atggaatccc gctaatcaaa tcacctagtt aaagcaacta gattgaatgt
      481 ttatcataag tctgatatca tgtttatg
//
LOCUS       DAIGOK010000277          481 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.289, whole genome shotgun sequence.
ACCESSION   DAIGOK010000277 DAIGOK010000000
VERSION     DAIGOK010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.289"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            137..>481
                     /locus_tag="NOA01_002761"
     CDS             137..>481
                     /locus_tag="NOA01_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633873.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          214 a           65 c           69 g          133 t
ORIGIN      
        1 taacatttac aaaattagct aaagctgaac aatatagacg atctttagaa ttgtcaaaag
       61 attaaatata taaaaagcct tcgggctttt tcttttaaca attaatagga acgtatattc
      121 gaaaggaata gaaataatgt ggttagaaaa aataaatgac aaaaaattta agtacaccga
      181 acgttatacg gatcccttaa ctgaaaaaaa gagaaaagtg tccgttactt tatctagcaa
      241 ttcacgccaa gcttggaatc aagctaactt acttttgaat gaaaaaatcg cagaaaaaat
      301 aaaacgaaat gaagaattac cactaacttt tggagaatta aaaactaaat gggatgaaaa
      361 atacaagcca actgtaaaag aaagttccta tcgaacaact caggtttatc tatcattaat
      421 ttcaaaatat ataaaagatg atgtacttgt aaaaaacgtt aatagtaatc ttatacaaga
      481 t
//
LOCUS       DAIGOK010000278          479 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.290, whole genome shotgun sequence.
ACCESSION   DAIGOK010000278 DAIGOK010000000
VERSION     DAIGOK010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.290"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..380
                     /locus_tag="NOA01_002762"
     CDS             <1..380
                     /locus_tag="NOA01_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCI0633874.1"
                     /translation="EICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQ
                     DLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQ
                     KFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          180 a           83 c           71 g          145 t
ORIGIN      
        1 aagaaatctg tgatgacttt aaggctgttt atcaagctaa ctcaaaagaa gaagcgaata
       61 ccttcttatc cggcatgatt gagaaatgga agaaaaacta tcctaaagtg acgcagtcac
      121 tcatagaaaa ccaagactta ttaacttttt atgattttcc acctagcatt cgtagaacca
      181 tttactcaac caatctaatc gagtctttca ataagcaaat taaaagatac agccgtagaa
      241 aagagcagtt tcaaaatgaa gaatcactag aacgctttct agtcagcatt tttgatacat
      301 acaatcaaaa atttctaaac agaagccata aaggttttca acaggtaacc gatacattag
      361 tttcaatgtt tactgagtaa ctaattattt tgcaggagga caatttattt acacaaaatt
      421 attgacgctc ccaaactgat cattaagttt ggcttttatg tcataattga attttataa
//
LOCUS       DAIGOK010000279          467 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.291, whole genome shotgun sequence.
ACCESSION   DAIGOK010000279 DAIGOK010000000
VERSION     DAIGOK010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.291"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            5..>467
                     /locus_tag="NOA01_002763"
     CDS             5..>467
                     /locus_tag="NOA01_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCI0633875.1"
                     /translation="MQTLVTKGGLNELFRSHLEKAINTLLRTELTAFLDYEKYDRTGF
                     NSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLETTIIHLFEK
                     GVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAIFM"
BASE COUNT          169 a           84 c           79 g          135 t
ORIGIN      
        1 aattgtgcaa actctagtca ctaaaggcgg tttaaatgaa ttattccgtt cgcacttaga
       61 aaaagcgata aacacactcc tacggactga attaacggct tttttagatt acgaaaaata
      121 tgatcgcact ggttttaatt caggtaattc gagaaacggt tcttactttc gatcaatcaa
      181 aaccgaatat ggtgaattaa cattggaaat acctagagat cgtaatggtg agtttaaaca
      241 acaaacttta ccagcctaca aaagaacaaa cgatacattg gaaaccacta ttatccattt
      301 attcgaaaaa ggtgttacga tgtctgaaat tgctgatttg atcgaaaaaa tgtacggtca
      361 tcactatact ccacaaacca tgtccaacat gactaaagtt ctgactgaag aagtaaatgc
      421 ctttaaatcc agagccttaa atgataagta tgtcgctatt tttatgg
//
LOCUS       DAIGOK010000280          465 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.292, whole genome shotgun sequence.
ACCESSION   DAIGOK010000280 DAIGOK010000000
VERSION     DAIGOK010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..465
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.292"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..>465
                     /locus_tag="NOA01_002764"
     CDS             <1..>465
                     /locus_tag="NOA01_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCI0633876.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQL"
BASE COUNT          194 a           55 c           65 g          151 t
ORIGIN      
        1 aagatatgct agattactac tattttgaaa aaaatttatc atataattat gtaatacatt
       61 taaaaacttt tacaggaatg atttttaaat tcgccaaccg tcgatatgga ttaaaatata
      121 atcccgtaaa tggggtttct ttagcaagaa aacctaagac aaacgaagaa ataaaaaaag
      181 aaaaagatgg ttatttatca aaagaagaag ttcaaatgat tgctagtaaa caaaaaagtt
      241 cacatcaaca gtcacgttat tctttaatta ctaaattttt gtttttaact ggactacgtt
      301 atggtgaatt gatatctttg actgaatctg attatgatgg taataaaatt acggtttctg
      361 gtacttatga ttacgagtta aaaattaaaa caacaactaa aaatacaggt tcttatagaa
      421 ccattgaatt atcaagtaat gctaaagaaa tcatagatca attaa
//
LOCUS       DAIGOK010000281          463 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.293, whole genome shotgun sequence.
ACCESSION   DAIGOK010000281 DAIGOK010000000
VERSION     DAIGOK010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.293"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(156..404)
                     /locus_tag="NOA01_002765"
     CDS             complement(156..404)
                     /locus_tag="NOA01_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005225422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCI0633877.1"
                     /translation="MHWLWVLIVGAIIGLIAGAITNKGGSMGWIANIVAGLVGSAIGQ
                     ALLGSWGPQLAGMALIPSIIGAIIVVAVVSFFLGRSRG"
BASE COUNT          157 a          101 c           68 g          137 t
ORIGIN      
        1 tattttttaa tctgacaaag gtaatgcttg cattcggcga catcagttag acaacatcga
       61 tgatcagtga tagaagcagt ttatctatac aaaaaaagaa gcagcgaaaa tctgctcctt
      121 aagataatag gtaaaattgc tgatatattt gacgattaac ctcttgaacg tcccaagaaa
      181 aatgatacta cagcaacaac gatgatcgca ccaatgatag aaggaatcaa agccattcct
      241 gctaattgag gaccccagct acctaacaat gcttgtccaa ttgctgaacc aactaaacct
      301 gcaacgatgt tagctatcca tcccattgat ccacctttat ttgtaattgc tccggcaatc
      361 aaaccgataa tagctcctac gattaatacc cataaccaat gcataatttt ctctttcttt
      421 ctgtgaaata tttattacac atattatgtt acgcttcttc tta
//
LOCUS       DAIGOK010000282          462 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.294, whole genome shotgun sequence.
ACCESSION   DAIGOK010000282 DAIGOK010000000
VERSION     DAIGOK010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.294"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..>462
                     /locus_tag="NOA01_002766"
     CDS             <1..>462
                     /locus_tag="NOA01_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HCI0633878.1"
                     /translation="GIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPDFNS
                     EFGHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNF
                     FKSITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGL"
BASE COUNT          152 a           93 c           84 g          133 t
ORIGIN      
        1 ggaatatttg atattgacac actaccgatg aaaggtaaaa gaaaacccaa tggccatcag
       61 gaaaaacggg gaaaacaaca gtatcagcgc tcaatccatg atcgacctga taattatcct
      121 gatttcaatt ctgagtttgg tcaccttgaa ggtgatacga tcgttggcat tcatcataaa
      181 agtgccgtca ttactttagt tgaaagatta tctaaagtca ttatcacgat taaacccaac
      241 ggccgtaagg cattagatat tgaaactgcc cttaatcaat ggttttctcg cttccctaaa
      301 aacttcttta aatctattac gtttgactgt ggaaaagaat tttctaactg gaaagccatt
      361 agtaaccaac atgatattga tatatatttt gcggaccctg gaacaccttc tcaacgccca
      421 ttaaacgaga attctaacgg gattctgcgt cgtaatggac tg
//
LOCUS       DAIGOK010000283          460 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.295, whole genome shotgun sequence.
ACCESSION   DAIGOK010000283 DAIGOK010000000
VERSION     DAIGOK010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.295"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(20..91)
                     /locus_tag="NOA01_002767"
     tRNA            complement(20..91)
                     /locus_tag="NOA01_002767"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="NOA01_002768"
     tRNA            complement(113..200)
                     /locus_tag="NOA01_002768"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="NOA01_002769"
     tRNA            complement(225..298)
                     /locus_tag="NOA01_002769"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="NOA01_002770"
     tRNA            complement(312..382)
                     /locus_tag="NOA01_002770"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="NOA01_002771"
     tRNA            complement(384..459)
                     /locus_tag="NOA01_002771"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DAIGOK010000284          449 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.296, whole genome shotgun sequence.
ACCESSION   DAIGOK010000284 DAIGOK010000000
VERSION     DAIGOK010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..449
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.296"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..>449
                     /locus_tag="NOA01_002772"
     CDS             <1..>449
                     /locus_tag="NOA01_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633879.1"
                     /translation="ESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPEEATQPITA
                     DDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPKDTKYTWKE
                     KVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          182 a           67 c          105 g           95 t
ORIGIN      
        1 gaaagtggaa caacagtctt tgcagtgaaa gacggcgtaa gcttaccaga agataaactt
       61 ccagtgttga aagcaaaaga tggttataca gatgcgaaat ggccagagga agcaactcaa
      121 ccaattacag cagatgatac agaatttgta tcaagtgcaa caaaactgga tgataaatct
      181 gacgctgaca aatataatcc tgaaggtcaa aaagtgacta cagaattgaa taaggaacca
      241 gacgcatttg agggaattaa gaacaaagaa gatttaccaa aagatactaa gtatacttgg
      301 aaagaaaaag tagatgttag cgcagctgga aataaaaaag ggactgttgt agtgacatat
      361 ccagatggat catctgatga agttgaagta gatgttacag tgacagacaa tcgttctgac
      421 gccgataaat atgagccaac agtagaagg
//
LOCUS       DAIGOK010000285          443 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.297, whole genome shotgun sequence.
ACCESSION   DAIGOK010000285 DAIGOK010000000
VERSION     DAIGOK010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.297"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..>443
                     /locus_tag="NOA01_002773"
     CDS             <1..>443
                     /locus_tag="NOA01_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCI0633880.1"
                     /translation="KTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTES
                     VRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNLF
                     LFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCN"
BASE COUNT          175 a           60 c           80 g          128 t
ORIGIN      
        1 ggaaaacaac cttactaaga atactcatga agattattcg ccctaccaaa ggaaagataa
       61 tcgaaaataa aaaaaatttg tcatcgccgt ttttagtaat gcaagaaatg gattaccaat
      121 ttttcacaga atcagtgagg agcgagttag ccctggggaa tgaaatagtt agtaaagatc
      181 agcaagaaaa aattttaaaa aaaatggaac tttataaaat gaaagatcaa ttaccgtttg
      241 atttatccgg tggagaaaaa caaagattat tggtttctat agcttcattg tcaaaaacta
      301 atttatttct atttgatgaa cctacaagtg ggttggatta tatcaatatg gaaaggattg
      361 ctgaccttat taaagactta aaagaaaagg gtgcagtagt tatagccact catgatccgg
      421 aatttcttta taaaacatgc aat
//
LOCUS       DAIGOK010000286          427 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.298, whole genome shotgun sequence.
ACCESSION   DAIGOK010000286 DAIGOK010000000
VERSION     DAIGOK010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.298"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(<1..>427)
                     /locus_tag="NOA01_002774"
     CDS             complement(<1..>427)
                     /locus_tag="NOA01_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCI0633881.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDEL"
BASE COUNT          125 a           76 c           87 g          139 t
ORIGIN      
        1 agttcatcca atgtgaaatc ttccgtcatg agtgaaatga gggttgcact aaaaagactc
       61 gtcgattcgt ctcgcatttc tcgagcaagc gtattacatt tgtacgttag attttcaaga
      121 aaatgttgct ttgtacgaat caattgctta ataagttgat agcgagtacg tgttaaacgc
      181 tgtaaggcca tgtatttctc ttctttgata ggggaagttg tgaatcgttg aatacgcaag
      241 aaatcagcga ttcttaatgc atcgtttcga tcagttttat tctcatcaaa catgcgacta
      301 aattgtttca cccgaaaagg attttcaatc gttacaagtg tgttgagctt ctttagtttc
      361 tcatcttcat gaaaaaacat ggaaggatgg aagctgtaca tggacgttga ctccatgcca
      421 atcacaa
//
LOCUS       DAIGOK010000287          423 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.299, whole genome shotgun sequence.
ACCESSION   DAIGOK010000287 DAIGOK010000000
VERSION     DAIGOK010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.299"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            22..279
                     /locus_tag="NOA01_002775"
     CDS             22..279
                     /locus_tag="NOA01_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633882.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          188 a           44 c           65 g          126 t
ORIGIN      
        1 ataaaaagga cgtgaagata aatgtcaaat ttagaaaaca aagaagaaaa agttgtaaac
       61 aaaattgttt ctgttgtcaa taaactggat aaagagttag acgaacttga tacgttatct
      121 gaaaatccag aaaaaaaaca taacctaaaa aaatggttag ttgaacgaaa ggctattcat
      181 gaaattaaaa aagttcttca tgaagctgat aaatatgaaa aatatgatga aaaagaactt
      241 gataaagaat ttaaagaaat caatgactta ttactataat tgatttatac gactcatttt
      301 tgagtcgtca gactgcaaac aacactctaa atttaggagt ttgtctacag tctgaataaa
      361 ttttaaattt attgtccata atgttattat gaatttatag cattaagaaa agagataaga
      421 ctt
//
LOCUS       DAIGOK010000288          417 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.300, whole genome shotgun sequence.
ACCESSION   DAIGOK010000288 DAIGOK010000000
VERSION     DAIGOK010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.300"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            95..>417
                     /locus_tag="NOA01_002776"
     CDS             95..>417
                     /locus_tag="NOA01_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HCI0633883.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKK"
BASE COUNT          147 a           86 c           68 g          116 t
ORIGIN      
        1 ccagcatctt ttttattttc caaaacatat agaaaagaca ccttgaaatc ctttagaatt
       61 aatttggcga accaacccaa aaggaggaat caagatgtct tacactcacc ttatcaaaga
      121 aacattagat attctagact tatcagttac ttttaacgaa aattgtctca caaaagaaaa
      181 atacaaaggg caaatctgtc atatttatcg tggaaacttg atttatacag cccaagaatg
      241 catccactgt aaacaccaga ttgcttcaga tattgtgcgt tggggtacaa caaccgtccg
      301 gttgttaatg aacgatgttt ctgaataccg tacctacctc gaattgaaaa agcaacgctt
      361 caaatgcaaa gcttgtcagc ggacctttgt cgcagatact tctgttgcga aaaaaca
//
LOCUS       DAIGOK010000289          394 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.301, whole genome shotgun sequence.
ACCESSION   DAIGOK010000289 DAIGOK010000000
VERSION     DAIGOK010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.301"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            40..>394
                     /locus_tag="NOA01_002777"
     CDS             40..>394
                     /locus_tag="NOA01_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCI0633884.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYE"
BASE COUNT          159 a           64 c           66 g          105 t
ORIGIN      
        1 tatgatatac tatctttaaa aataaaaagg agaaaaaaga tgaagccaaa tccttcagaa
       61 gcaggaaaac gaatcaaaca attacgcctt tcatgtggtt ttactatgga agaacttgga
      121 aggaaaatag ataattcacc acgtgcgacc atatccaact gggaacgtgg gaccaattta
      181 ccaaatccac aaaagctgaa attattatca actatcacta attcaacaat tgactggatt
      241 aaatggggaa ctttagaaga atatataact tcatacctta tcgatatagg gtatgagcta
      301 tatattaagg actttccaga aatacctcac aaagtcttta aagacattca agaaagatat
      361 agtgatacgt tttcgctaga taaagactat gagt
//
LOCUS       DAIGOK010000290          381 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.302, whole genome shotgun sequence.
ACCESSION   DAIGOK010000290 DAIGOK010000000
VERSION     DAIGOK010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.302"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(21..94)
                     /locus_tag="NOA01_002778"
     tRNA            complement(21..94)
                     /locus_tag="NOA01_002778"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="NOA01_002779"
     tRNA            complement(108..181)
                     /locus_tag="NOA01_002779"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="NOA01_002780"
     tRNA            complement(209..281)
                     /locus_tag="NOA01_002780"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="NOA01_002781"
     tRNA            complement(301..374)
                     /locus_tag="NOA01_002781"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
BASE COUNT          103 a          108 c           90 g           80 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat t
//
LOCUS       DAIGOK010000291          380 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.303, whole genome shotgun sequence.
ACCESSION   DAIGOK010000291 DAIGOK010000000
VERSION     DAIGOK010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.303"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="NOA01_002782"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="NOA01_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HCI0633885.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          121 a           57 c           64 g          138 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttgtatagac tgattgtaac atgggacaaa ataaaaaggc
      361 aatacgcctt agttgtcggt
//
LOCUS       DAIGOK010000292          349 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.304, whole genome shotgun sequence.
ACCESSION   DAIGOK010000292 DAIGOK010000000
VERSION     DAIGOK010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.304"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(44..>349)
                     /locus_tag="NOA01_002783"
     CDS             complement(44..>349)
                     /locus_tag="NOA01_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCI0633886.1"
                     /translation="TIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQF
                     ETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT           98 a           86 c           64 g          101 t
ORIGIN      
        1 atcctcggat ctgattttct ccggcagaag cacaatcatt caattacgct gcctctccag
       61 aggtatcaga tccgcgctca ttatcaagga ttcgcttcgc caatctctgt tgtcgaattt
      121 ggatcggtgt ttcatacgcc aacgtgccat gtaagcgtag atagttccac caatgcacat
      181 aatcaaacaa ttctagacgt agctgtgcca gtgtctcaaa ttggtattga tgcacgaatt
      241 ctactttgac agatttatag gttgattcca caacggcatt atcatacgga caacccttcc
      301 tactcaatga acgagtaatt ccaaaaccat tcagaatctc atgaatggt
//
LOCUS       DAIGOK010000293          340 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.306, whole genome shotgun sequence.
ACCESSION   DAIGOK010000293 DAIGOK010000000
VERSION     DAIGOK010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.306"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
BASE COUNT          139 a           47 c           39 g          115 t
ORIGIN      
        1 ataacttgaa aaatcgaaaa aaactcattt tgtgtcaagt tatctgtaat atataaaaat
       61 atcattatta tacctcatct atgtgaattt ttactattct taccttagca tattctttat
      121 aggtatatca gagctaatca aactaaaaaa tacccgaagt cttcttatgt aaaaacaccg
      181 gttattactt aaatatgact taggtaaaac aatgagtgat ccaattataa aattttatgg
      241 cgaataattt agtaagcgct acatattgaa tgatttagaa attttgaaaa agtagctagt
      301 tccttttata ccagaaaagt aaacctaata aaaaacacaa
//
LOCUS       DAIGOK010000294          334 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.307, whole genome shotgun sequence.
ACCESSION   DAIGOK010000294 DAIGOK010000000
VERSION     DAIGOK010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.307"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..318
                     /locus_tag="NOA01_002784"
     CDS             <1..318
                     /locus_tag="NOA01_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HCI0633887.1"
                     /translation="IIKIVLSASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAF
                     NYQDKVQTSTELLMKIEKEKLLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQ
                     VNN"
BASE COUNT          135 a           47 c           39 g          113 t
ORIGIN      
        1 attataaaaa tagtcttatc agctagtatc ccaatattaa ttgatcaggc ttccgaacac
       61 aggtctttac tattgattgt ttcaattgct tcagcaataa ttacaattat acaaagtggt
      121 atgtctgcat ttaattatca agataaagta cagacttcga cagagttatt aatgaaaatt
      181 gaaaaagaaa agttgttata tataacgaaa acatccccat ataataaaac agatgaagaa
      241 aatttccatt taatcgttac aacccttcaa actgaattaa atgatattat ctctgatttt
      301 aatcaagtta ataactaaaa atttatttaa ttag
//
LOCUS       DAIGOK010000295          334 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.308, whole genome shotgun sequence.
ACCESSION   DAIGOK010000295 DAIGOK010000000
VERSION     DAIGOK010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.308"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            78..>334
                     /locus_tag="NOA01_002785"
     CDS             78..>334
                     /locus_tag="NOA01_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HCI0633888.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRL"
BASE COUNT          118 a           52 c           70 g           94 t
ORIGIN      
        1 taggtattaa aaaacatatc gttctctagt atgattaaat cacgacaaaa taaacaactg
       61 agaggacgat atgctatatg aatgattcta tcaaaaaaat gctgcgatta atagagaaag
      121 atttgatgat tacagaggtc tcttacgaga cctttcagaa gaaaaagacg ttgatcgtcg
      181 atgctgtttt ctcacctgct ccccatacct gtagaaattg tggttctact gtggtagatg
      241 gaaacgggaa agtaattgta gtgaaaaatg ggaagaagga aacgattgtc cgctttgaac
      301 aatacaatca catgcctttg gttatgcgct taaa
//
LOCUS       DAIGOK010000296          321 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.309, whole genome shotgun sequence.
ACCESSION   DAIGOK010000296 DAIGOK010000000
VERSION     DAIGOK010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.309"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(69..321)
                     /locus_tag="NOA01_002786"
                     /pseudo
     CDS             complement(69..321)
                     /locus_tag="NOA01_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DAIGOK010000297          319 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.310, whole genome shotgun sequence.
ACCESSION   DAIGOK010000297 DAIGOK010000000
VERSION     DAIGOK010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.310"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            66..>319
                     /locus_tag="NOA01_002787"
     CDS             66..>319
                     /locus_tag="NOA01_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCI0633889.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDT"
BASE COUNT           94 a           57 c           62 g          106 t
ORIGIN      
        1 ggatgatgtc tacagtcttt tcttttataa tagatttatg aaaacgaatg caaggagtga
       61 tagctatgat gtctaaaaac aatacgaatg gtagaaatca atttgcgatg cttacgattg
      121 atgatctagt tcctcaagat catctcgttc gaaagattga tgccgcgctt gattttgaat
      181 ttatttatcc aatcgtcgaa gccacttact ccgatttagg tcgcccaagt atcgatccag
      241 tgattctgat caaattggtg tttattcagt atttattcgg tatccgttcc atgcgacaaa
      301 ccattaagga agtcgatac
//
LOCUS       DAIGOK010000298          295 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.311, whole genome shotgun sequence.
ACCESSION   DAIGOK010000298 DAIGOK010000000
VERSION     DAIGOK010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.311"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(119..265)
                     /locus_tag="NOA01_002788"
     CDS             complement(119..265)
                     /locus_tag="NOA01_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCI0633890.1"
                     /translation="MILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
BASE COUNT           97 a           69 c           24 g          105 t
ORIGIN      
        1 cttatttatc actaatgttt caaacggaac aactttattt tcctcagcca ttctcccagt
       61 tccattttca aaaatcacaa aactgatatt accttaaaaa ctactaacaa aataaaactt
      121 ataaagtaaa tggcaaccca actatgtaac cattctctct acgtactacg atttggtagt
      181 cctgcctttc aatcggtaat tcataatagt aatttactgt ttcttctttg ccactttcaa
      241 tatactctgg attatccaat atcatatcat actcataaag ttcttcacca tcctt
//
LOCUS       DAIGOK010000299          287 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.312, whole genome shotgun sequence.
ACCESSION   DAIGOK010000299 DAIGOK010000000
VERSION     DAIGOK010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.312"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..>287
                     /locus_tag="NOA01_002789"
     CDS             <1..>287
                     /locus_tag="NOA01_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HCI0633891.1"
                     /translation="QRATYRAVCSVLFPNGDFPSRSRFTRLSSNLAYTIKIIRYFFIK
                     KLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYFYGLKI"
BASE COUNT           98 a           53 c           45 g           91 t
ORIGIN      
        1 caaagagcca cgtatagagc ggtttgttcc gtcttatttc ctaatggtga ctttcctagt
       61 aggagtcgat tcactcgctt aagttcaaac ttagcttaca ctatcaagat tattcgatac
      121 tttttcatca agaagctcac caaaggtgaa ctagtcggaa ttatagatag ttttcctagt
      181 ccattatgta aaccagttag aaatagacaa gcaaaactat tgaatcaaat agccaaagtt
      241 ggctataact caacaaaaaa atcttatttt tatggtctta aaattca
//
LOCUS       DAIGOK010000300          279 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.313, whole genome shotgun sequence.
ACCESSION   DAIGOK010000300 DAIGOK010000000
VERSION     DAIGOK010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..279
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.313"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..270
                     /locus_tag="NOA01_002790"
     CDS             <1..270
                     /locus_tag="NOA01_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCI0633892.1"
                     /translation="ISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLT
                     ELGIPLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          120 a           49 c           33 g           77 t
ORIGIN      
        1 atctctaaaa atggtaaccc gatatctatt caagcatata atcaatcttt aagagcagta
       61 tccaaagaat taaacataga taaaaaagtt tcttctcaca tgttaaggca ttcccacatt
      121 tctctactta cagaattagg aatcccatta aaagccatta tggatcgtgt aggacatgaa
      181 gattcaaaaa caacattaaa gatttatact catacaacta aaaatatgca aaatcaacta
      241 gtagaaaaac taggaaaaat agaaatttaa attgcccct
//
LOCUS       DAIGOK010000301          268 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.314, whole genome shotgun sequence.
ACCESSION   DAIGOK010000301 DAIGOK010000000
VERSION     DAIGOK010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.314"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
BASE COUNT           72 a           52 c           53 g           91 t
ORIGIN      
        1 agcctagcgt gagcggggcg gattgactgg caagtagatg ggtagctggc gtatgtccta
       61 aaaactgctt ctttccaagc aactcttcat aggcaaaaaa ctggaaagtc cactaacaag
      121 cccattcaga attccatttc aaaattttgt tgcctttttt gctgattttc aatcatttgt
      181 tgtgtgaatt cataatcctt tttagcacga tacattttta catcgtcatt cattatgcga
      241 ctgatttgat agtgaatctg tttagcag
//
LOCUS       DAIGOK010000302          265 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.316, whole genome shotgun sequence.
ACCESSION   DAIGOK010000302 DAIGOK010000000
VERSION     DAIGOK010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.316"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..>265
                     /locus_tag="NOA01_002791"
     CDS             <1..>265
                     /locus_tag="NOA01_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HCI0633893.1"
                     /translation="KAGFVTEDNLYLDSTHIKANANKHKFTKEMTHDEAKAYQDELED
                     EINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVKGER"
BASE COUNT          106 a           44 c           61 g           54 t
ORIGIN      
        1 aaagcaggtt ttgtaacaga ggataatttg tatctggatt ctacccacat caaagctaac
       61 gccaataaac ataagtttac gaaagaaatg acccatgatg aagcgaaagc ctatcaggat
      121 gaattagaag atgagatcaa tcgtcaacga atagaggctg gaaaacgccc ttttactttg
      181 gacttagaaa aggaagtgaa gctgaaagaa agaaaaataa gtaaagctga tccggaaagc
      241 ggctactacg tcaaaggcga acgag
//
LOCUS       DAIGOK010000303          263 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.317, whole genome shotgun sequence.
ACCESSION   DAIGOK010000303 DAIGOK010000000
VERSION     DAIGOK010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.317"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            25..>263
                     /locus_tag="NOA01_002792"
     CDS             25..>263
                     /locus_tag="NOA01_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCI0633894.1"
                     /translation="MIESYYLQHNKPVEIANRMGRAIQTIYNVVNKFKQGKTALDYWH
                     QYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDV"
BASE COUNT           99 a           45 c           49 g           70 t
ORIGIN      
        1 catcttacca tagacgaact gacaatgata gaatcatatt atcttcaaca taataagccg
       61 gttgaaatcg ctaaccgaat gggacgtgct atacaaacta tttataatgt agtcaataag
      121 ttcaagcaag gcaagactgc tcttgattat tggcaccagt ataaagaaaa taagaaaaaa
      181 tgtggtagaa aagtcattca attacctgct catgaagtag attacattaa agagaaagtc
      241 actcttggtt ggacgcctga cgt
//
LOCUS       DAIGOK010000304          260 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.318, whole genome shotgun sequence.
ACCESSION   DAIGOK010000304 DAIGOK010000000
VERSION     DAIGOK010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..260
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.318"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            complement(<1..>260)
                     /locus_tag="NOA01_002793"
     CDS             complement(<1..>260)
                     /locus_tag="NOA01_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCI0633895.1"
                     /translation="EDIYKIGMTRRLEPMDRIKELSSASVPFEFDVHAMIFSEDAPSL
                     ETKLHNHFRKQEVNKINQRKEFFKVSLDEIEKVVLENYNGTVT"
BASE COUNT           78 a           48 c           28 g          106 t
ORIGIN      
        1 gttactgttc cattataatt ttctaaaaca actttctcta tttcgtctag tgaaactttg
       61 aaaaattctt ttctttgatt aattttatta acttcttgtt ttctaaaatg attatgcaat
      121 ttagtttcca aagacggagc gtcctctgaa aaaatcatcg catgaacgtc aaattcaaat
      181 ggaaccgatg cactactcaa ttcttttatt ctatccatag gttctaatct tctagtcata
      241 ccaattttat atatgtcttc
//
LOCUS       DAIGOK010000305          253 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.319, whole genome shotgun sequence.
ACCESSION   DAIGOK010000305 DAIGOK010000000
VERSION     DAIGOK010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.319"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            13..>253
                     /locus_tag="NOA01_002794"
     CDS             13..>253
                     /locus_tag="NOA01_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCI0633896.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLF"
BASE COUNT           81 a           51 c           65 g           56 t
ORIGIN      
        1 aggatgatct caatgaaaaa aatgtacgaa acagcaatga tcaatcgtgg aggccgtgat
       61 ggcgaggttg aggcaccaaa cggaagtatg cacatgaaga tcgaccgccc aggaatccat
      121 agtgaaggga caaatccgga acaacttttt gcggctggat acgcatcttg cttcaacggc
      181 gcggttcaac acatgctgga agaaaataat cttgagtctg attctgaagt caaagcacgg
      241 gtatctttat ttc
//
LOCUS       DAIGOK010000306          248 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.320, whole genome shotgun sequence.
ACCESSION   DAIGOK010000306 DAIGOK010000000
VERSION     DAIGOK010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.320"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
BASE COUNT           85 a           43 c           31 g           89 t
ORIGIN      
        1 ctttctgatt ttgatttttg ccagtaattt taaaccctgt tgaaagatat ccgtctacta
       61 accgagcatt ggccaaatta ataagtcgag gataatcttt ctttacttca tcaactaata
      121 tttttctctg caaaatatta tcatactgtt ttgaattaaa agactgataa acgatgctat
      181 cgttattttg ataataaaaa atacctgaaa atggcaaaat atcaatttta ctagccgccc
      241 taaaagct
//
LOCUS       DAIGOK010000307          247 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.321, whole genome shotgun sequence.
ACCESSION   DAIGOK010000307 DAIGOK010000000
VERSION     DAIGOK010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.321"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
BASE COUNT           93 a           30 c           37 g           87 t
ORIGIN      
        1 taccgaaaaa attatgttta gataatacca tagttaagtt tttccttgtg atgtttttcg
       61 taaaaagata ttttagcagc attatcgtta cagtgcatag ttaccatcgg cgttatgact
      121 atataaagta tactttcgtt gatccagaaa ggtatataga ttaaaataag ttacctaaaa
      181 aggacaaata caaattttat tatcttattc acagtaagaa gataatttta agattgcgaa
      241 atacgat
//
LOCUS       DAIGOK010000308          240 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.322, whole genome shotgun sequence.
ACCESSION   DAIGOK010000308 DAIGOK010000000
VERSION     DAIGOK010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.322"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..129
                     /locus_tag="NOA01_002795"
     CDS             <1..129
                     /locus_tag="NOA01_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCI0633897.1"
                     /translation="QNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE"
BASE COUNT           84 a           39 c           40 g           77 t
ORIGIN      
        1 caaaatgaag aatcactaga gcgtttccta gtatccatct ttgatacata caatcaaaaa
       61 ttcttaaata gaagccataa aggcttccaa caagtgacgg atacattagc ttcaatgttt
      121 actgagtaac ccattatttt gcagaaggac gagttattta cacaaaatta ttgacgctcc
      181 ctgtttggag tggtaaaaag attgagaaat tgtttcatta gctattacga ttattattgt
//
LOCUS       DAIGOK010000309          233 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.323, whole genome shotgun sequence.
ACCESSION   DAIGOK010000309 DAIGOK010000000
VERSION     DAIGOK010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.323"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..>233
                     /locus_tag="NOA01_002796"
     CDS             <1..>233
                     /locus_tag="NOA01_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HCI0633898.1"
                     /translation="AFQLVKQSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPY
                     SRPKGKKGLLSKNEFLYDEYFDCYICPQDQMLA"
BASE COUNT           62 a           46 c           45 g           80 t
ORIGIN      
        1 tcgcctttca gctcgtcaaa caatcgaaga gactttttcc tgaaatcaat tgtgttgtcg
       61 ctgatgctgg gtataagaca cctaagtttg ttcacttttt gactcatttg aaccttcgac
      121 cttgtttgcc ttattcaagg cccaaaggga aaaaggggct attatcaaaa aatgagtttc
      181 tctatgacga gtattttgat tgttatattt gtccgcaaga tcaaatgctc gct
//
LOCUS       DAIGOK010000310          227 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.324, whole genome shotgun sequence.
ACCESSION   DAIGOK010000310 DAIGOK010000000
VERSION     DAIGOK010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.324"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            1..227
                     /locus_tag="NOA01_002797"
                     /pseudo
     CDS             1..227
                     /locus_tag="NOA01_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT           94 a           43 c           44 g           46 t
ORIGIN      
        1 tacgccttct tctttcgata aatggatgaa gcaagcacaa tcaacgggct cattcaaaga
       61 aagagacaac ttaacaccag aacaagcaga attgatcgca ctaagaaaga aaaataagca
      121 actcgaaatg gagaatgata ttttaaagca agcggcgctg atattcggac gaaaagacaa
      181 gtaattgatg ccaacaagca taaatattcc atatcagcga tgtgcaa
//
LOCUS       DAIGOK010000311          226 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.325, whole genome shotgun sequence.
ACCESSION   DAIGOK010000311 DAIGOK010000000
VERSION     DAIGOK010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..226
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.325"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..226
                     /locus_tag="NOA01_002798"
     rRNA            <1..226
                     /locus_tag="NOA01_002798"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           66 a           38 c           66 g           56 t
ORIGIN      
        1 gatggactta ccgctggtgt accagttgtt ctgccaaggg cattgctggg tagctatgta
       61 gggaagggat aaacgctgaa agcatctaag tgtgaagccc acctcaagat gagatttccc
      121 atttctttaa gaaagtaaga tccctgagag atgatcaggt agataggtca ggagtggaag
      181 tacagtgatg tatggagcgg actgatacta atcgatcgag gactta
//
LOCUS       DAIGOK010000312          224 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.326, whole genome shotgun sequence.
ACCESSION   DAIGOK010000312 DAIGOK010000000
VERSION     DAIGOK010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.326"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..>224
                     /locus_tag="NOA01_002799"
     CDS             <1..>224
                     /locus_tag="NOA01_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCI0633899.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DAIGOK010000313          217 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.327, whole genome shotgun sequence.
ACCESSION   DAIGOK010000313 DAIGOK010000000
VERSION     DAIGOK010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.327"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            33..>217
                     /locus_tag="NOA01_002800"
     CDS             33..>217
                     /locus_tag="NOA01_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HCI0633900.1"
                     /translation="MKITEEKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSE
                     ISKLIAYMQKANADVEK"
BASE COUNT          104 a           27 c           37 g           49 t
ORIGIN      
        1 ttcaagaact agaacttcat tactcttatt tagtgaaaat taccgaagaa aaagaacaac
       61 aaaaagcaat aaaagaacag atgatagaag aggaaaaagt tcgtagagaa attgatcgtg
      121 aaaagaaacg tattgataaa gaacaacgtc aattcaattc tgaaatttcc aaattaatcg
      181 catatatgca aaaagctaat gcagatgtgg aaaaaga
//
LOCUS       DAIGOK010000314          211 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.328, whole genome shotgun sequence.
ACCESSION   DAIGOK010000314 DAIGOK010000000
VERSION     DAIGOK010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.328"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
BASE COUNT           76 a           32 c           39 g           64 t
ORIGIN      
        1 gagtttaaag cgtaatacac ttatggggaa gtggtttggg gtgcgcttta aactcttctt
       61 tattattatc tcacagcatg cttaaaaggt ctatggatta agttatgtaa aaaagtgcct
      121 ctttaaacgt cctaaaatcc aaacaaggat agcataatgt acactatcgt tgatccagaa
      181 aacaggtaaa taaattttgc aaaagcaata a
//
LOCUS       DAIGOK010000315          209 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.denovo.329, whole genome shotgun sequence.
ACCESSION   DAIGOK010000315 DAIGOK010000000
VERSION     DAIGOK010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..209
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.denovo.329"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..>209
                     /locus_tag="NOA01_002801"
     CDS             <1..>209
                     /locus_tag="NOA01_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCI0633901.1"
                     /translation="RWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRT
                     PLPKVLCFDEFKSVRGVSGAMSFIM"
BASE COUNT           51 a           43 c           44 g           71 t
ORIGIN      
        1 cgctggtctg tcgtgactcg tctgaaaaaa aatacctcaa tgacagagat cgccgctcag
       61 aaaaatctat cggtctcttc tgtttaccgt attatgaagc gattttatcg tccgttgaat
      121 ccttttcgga caccgttacc taaggtactt tgttttgatg aattcaaatc agttcggggt
      181 gtttcaggtg ctatgagttt catcatgat
//
LOCUS       DAIGOK010000316        10369 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.guided.097, whole genome shotgun sequence.
ACCESSION   DAIGOK010000316 DAIGOK010000000
VERSION     DAIGOK010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.guided.097"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            183..1079
                     /locus_tag="NOA01_002802"
     CDS             183..1079
                     /locus_tag="NOA01_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HCI0633902.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(1229..2170)
                     /locus_tag="NOA01_002803"
     CDS             complement(1229..2170)
                     /locus_tag="NOA01_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCI0633903.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            2296..3417
                     /locus_tag="NOA01_002804"
     CDS             2296..3417
                     /locus_tag="NOA01_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCI0633904.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            3675..5177
                     /gene="eat(A)"
                     /locus_tag="NOA01_002805"
     CDS             3675..5177
                     /gene="eat(A)"
                     /locus_tag="NOA01_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HCI0633905.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            5205..5771
                     /locus_tag="NOA01_002806"
     CDS             5205..5771
                     /locus_tag="NOA01_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCI0633906.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            5982..7043
                     /locus_tag="NOA01_002807"
     CDS             5982..7043
                     /locus_tag="NOA01_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCI0633907.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            7046..7942
                     /gene="murQ"
                     /locus_tag="NOA01_002808"
     CDS             7046..7942
                     /gene="murQ"
                     /locus_tag="NOA01_002808"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCI0633908.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            7956..9938
                     /locus_tag="NOA01_002809"
     CDS             7956..9938
                     /locus_tag="NOA01_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCI0633909.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(10003..>10369)
                     /locus_tag="NOA01_002810"
     CDS             complement(10003..>10369)
                     /locus_tag="NOA01_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCI0633910.1"
                     /translation="AEDPVNPEVTLKEIHENSDFTKETEPFFTFVPTYLDGGNGLDNG
                     DTEILTETMREYLEHEDNHKLCLGVVGSGNKNFNNQYCLTAKQYAQTFGFPFLADYEL
                     RGTPSDVTRIYQILAENNK"
BASE COUNT         3399 a         1833 c         2243 g         2894 t
ORIGIN      
        1 ttcagacaat tacaactatt cagaaaactt ttaggcgaat tttaatagaa gaatgattta
       61 tttcatcata ttttcataca aatgttaaat tttcatacaa atataggaaa cgttttactg
      121 gctttttact atttaaacag gctatcatag gaaaaagtga cagagaaagt gaggcggaaa
      181 agatgaacaa aaatgacgta ctgctagttg caaaaaataa atcactttat tttttgcctt
      241 ccatccgctc tttagatgtt tggtccggaa aagatttact acagtcggaa ttcattaaat
      301 cagctagaca acagttggca aattatcagc tgatcgtttt tttagattat ggatttgata
      361 tggagatggc tcgacgcgtg cgcccattta ccaatgcaaa aattgtgtta tttttttgga
      421 atcacttcaa agcagaacac gaggcgatgc tgaagcaggc acaagatgaa ccggcaatcg
      481 atgagattta tcattttgat tttttggaag caaaagaact tgggctgaaa cataatagtt
      541 cattttattc gaaacatatg aatttttctt ctagcgaacc aaagacggat ctattttttg
      601 gtgcaacgaa taatgggaga aaagaacgag cagaaagcta taagcaggaa tttataaaaa
      661 gagatgttac gaccaactat ttcattcttc cacatcgagg gaatgaacaa cctggatatc
      721 ttacttatga agagtacctg aaaaaaacag gggaatcgcg cgggattctg gaactgttgc
      781 gggagggtca gcaaggagtg actttgcgga catttgaatc catgtatttt caaaagaaac
      841 tagtgacgga taatcaagcg attgctcatt atcattttta tcgtcctgaa aatatttttt
      901 tgcttcgaga acgtgattta gatgaattac cggaatttct gcatacacca tttaagccaa
      961 tcctatcaga gctattggaa ttttatgatg cagaaaattg ggcaaaacga tttatcacta
     1021 acgattccac tatttatgag caatacgaat atgacctctc ctctgttgaa aacaaataaa
     1081 aaacctacat tttgaagaga ttatgtactg aaacaaacaa gcacaattct tatggcatca
     1141 tgagagttgt gttttctttt tgatctttta ttgggcttaa aaaaagagaa ccagctgctg
     1201 attcagctga ttctcatcag agagcctttt attctgcgcc aaggattcct tcttttgcat
     1261 ggactaattg ggtcaaaaag gcacaatatg gtgtagcaat atcatatttt tgtccttttc
     1321 gccaaatggc accattgatg tagtcgattt cagtcaaacg atggtttttg atcaggtctt
     1381 ggtacataga tgggtaatgc aacccgatgt tgtcaggatc ataagtagaa gcaatatgtt
     1441 ggtacacttc ttcttgatct agtatgattc cttcttttgc tgcgatagaa gcaaattcag
     1501 atacgattgt tcgaaccatg ctctctgccg ctttttgcgc gcctagttca gcaatattac
     1561 aatccaaaat cgtgcataaa ccattcaatg ttccatttac acaagctttt cgccaaatgg
     1621 aataacgaac gttatggcta tagatcggat ttaaatttgc ttcttgaaag acttctacaa
     1681 ctttttttgt aaatgcttcg ccttctggtt ccaagttctc tagttcgatt tctccatcac
     1741 ctaacaatgt gacttttccg ggaccagcta gaccagctgt ccacatcgtg atcccaaata
     1801 agatattttt ctttggcaca tatttttcca gtacatcttc atgacctaat ccatttagta
     1861 aacacaaaac ataagtattg tcagtaataa tggattggat cgaacaaaac atatcatcta
     1921 attgattggc ttttgttaat gctacgatca agtccacgtg ttcgttgctt tcgatgattt
     1981 cttctggtga atagattggc agttttgcga ccacttcttt accgttaaag tcagcaatca
     2041 acccatttgt acgaatggct tcaatgtgag caggccaacg atcgatcaag aggacctcat
     2101 ttccgctttg atgtagcatc aatccgattc ggctgcccat tgctccggcc ccagctattg
     2161 cgattttcat gttttttctc tcctttattc aatgagttcc cttttagtat atagaaaaat
     2221 ggaagaaaat agcattgttt tgtttgaaaa aaatgaaaaa aagtgtctta cttaagaaga
     2281 aagtgaggga tacagatgga tattttggcg gcaattgatt catttaaagg aagtgcttct
     2341 tcatttgaac taaatcaggc agttctaaat ggctgggaag atcgtaaagg aagaaaaatc
     2401 aatgtgccga ttgcagatgg cggagaaaat actgcagaag caatctatta cgcgctcagg
     2461 ggaacatggc gtaccattga tgggatggat ctcttattcc gtcagcgaaa aatcagctat
     2521 ttattgacaa cttggcaagg aaaaaacacc gcagtcatcg aatctgcaca agcattgggg
     2581 ttagatctac ttgataaacc aacggatcaa actatccgac ttgcttccag ttatgcctta
     2641 ggagaactta ttaaagatgc actgcttcaa gaagtagaac aaattattgt cactttagga
     2701 ggaagcgggt gtagcgatgg tggtctagga ttactccaat ccttgggtgc ttctttagaa
     2761 gggtacacag aaggaaatcc tttattatct actaaaaaac ttcattttga tcgaagaaac
     2821 gttcttgttt ctaaagtaaa attgattatc gctgcagatg ttaccaatcc atatacaggt
     2881 caacaaggag ctgcttggca atttggaaga caaaaaggag gaatagatac gacgctagct
     2941 ttcttagata aacaagcagg aaagattgct cagcaaataa aagatttctg ggaggtggat
     3001 ttagatgata ttccaggaag tggagcagca ggcggtattg gtggagccct ttttttgttg
     3061 ggagcgaaga tggtttctgg tttcgattta gtcagccaaa tcactggatt ggaaaaaaag
     3121 atcgaacaag cagatgtcat cattacagga gaagggcgga tcgatcagca aacgatccac
     3181 ggaaaagttc catacggtgt agcagtgctt gcaaaaaaat atcgaaaacc aataatcgct
     3241 atttgcggaa gtagagacca agagctagaa ggactttctg attttctccc aattgtctta
     3301 agtattcatt tgggtccaat aggtttagaa gaagcaatgg aacacaggca aacattagaa
     3361 aaaacaaaaa tcttaggaca gacacttagc agatttttca ccatcctccc gaagtagttg
     3421 acaaaatcat tttctttatg ctatcgtaat ttcaaatata ggacaaagtg agggatccgt
     3481 gcattgaaaa attaagtcga ttgatgaaag ctgcattcag actgtacctt gtggagacaa
     3541 ggaagcagtg tgcagatttt cgggattggc ttggtttttt cgatgtatgg gtctttttgt
     3601 gtgtatccaa cccttgcatc ctctttagcc agttctgaaa ataggactgg tcttttttta
     3661 cgaggtgatg gaaaatgtct aaaatcgaaa taaaaaatct gacattcggc tacgacagcc
     3721 aaggcacatt attatttgaa caagcaaatc taaattttga cacacaatgg aaactaggac
     3781 ttatcggacg aaacggtcga ggaaagacaa ctttactgaa tattctacaa aacaaactac
     3841 cttatcaagg gcaagtaatc catcagcaag aatttgccta tttcccgcaa cagacaaaag
     3901 ataaagaacg tttaacctat tacgtgttaa atgatattac ggattttgag atatgggaaa
     3961 tcgaaagaga gctccaattg atgcaaacag atcctgaaat cttatggaga gaattcagca
     4021 cactatcggg gggagagaag acaaaagtcc tactggcact tttatttgtg gatgacactc
     4081 atttcccgtt aatcgatgaa ccaacgaatc atttggatat ctctggtaga aaacaagtag
     4141 cggcttattt gaaaaagaaa aaacaaggct tcatcgtggt cagccatgac cggggattta
     4201 tcgatgaagt agtggaccat gttttagcaa tcgaaaaaag tcaactggaa ctttatcaag
     4261 ggaatttctc tatctatgaa gaacagaaaa aacttcgtga tgaatttgaa atggctcaaa
     4321 atgaaaaatt gaaaaaagaa gtcagtaggc taaagaaaac agcagctgaa aaagccgaat
     4381 ggtctcgttc ccgagaagga gataaaacaa agaaacaagt cggattcatc gatactgaat
     4441 ctagacgagt gaataaagga gcagtgggtg ctgatgctgc acggacgatg aaacgatcca
     4501 aagcaatcgt gaatcggatg gagacccaga tcagcgagaa agaaaaacta ttaaaagata
     4561 tcgaatatat cgattctttg acgatgaata gccaagcgtc tcaccataag cgacttttaa
     4621 gcgtagaaga tcttcaatta gggtatgaaa atctgttatt cgagccaatt cattttacaa
     4681 tcgagcctca tcagcgggtg gcgatttcag gtcctaacgg tgcaggaaag tcatccatta
     4741 tccattatct tctgggggca ttcaacggca aggttatagg agaaaaatac cagccaaaac
     4801 atctgagcat tagttatgca agccaaaatt atgaagacaa tcgaggaacg ttggcggaat
     4861 ttgcagagaa aaaccaagta gactaccaag catttttgaa caacctccga aagcttggga
     4921 tggaaagaga tgtttttcat aacaagatcg agcagatgag tatgggccaa cggaaaaaag
     4981 tggaattggc taaatcttta tcacagccag ctgaactata tacatgggat gaaccattga
     5041 attatttgga tgtcttcaat caagaacaat tagaacaact gatcttgaac gtgaaacctg
     5101 ccatgttact agtggaacat gatcaaacct tcctggataa agtatctact gagattattt
     5161 ctcttgagag aatctaaccc ttgtaatctc aagaacaaca aggtatggtt aaggggaact
     5221 ttgccaacga aggagagaat cacatgcttt atgtaacgag acacggagaa acaacttgga
     5281 acgcacaagg attagtttgc ggacgggcag atgtgccgct gactgaaaaa ggacagatgc
     5341 aggcacagaa actagcagaa aaggtagtag atttgcctgt tccaatcacg aaaatcatcc
     5401 attcccctct tcaacgagca agagatacag cacaagcagt tgctgatcgt ctcagcttgc
     5461 cgctgaccgt tgatgaacga ttggtagaaa tggattttgg ggattatgat ggactgccaa
     5521 gcaaagatga gaactttcaa aaagcacgtt tagcgttcgc tgtacgattc ccaaatgggg
     5581 agtcagtctt agatgtgtat gcacggatcg ttcctttatt gaaagaatgc atcgaagacg
     5641 aagaaaatgt ctatctgtta gtttgccata atgccttgat aagagtgatc aatgcttatt
     5701 ttcatccgat gcctaatgag gggttcttta cattcatggt agataataca gaattggttt
     5761 cttatgagta attatgtttt tgtgttattt aaataaaata tttcaaggca ctgcttttgc
     5821 ttagcagtgc cttttttagg ttatgacaat tcttttgata atataaactc aacctttaga
     5881 aatgaaattt aattatttta ataaaagtat tgaaattttg tttctttaga tatatactct
     5941 agtcatagaa atgaagacgg atacagaaaa agagggataa tatgtttggc ttttcaatat
     6001 ttatgaacga ttctttgtca gaagagaaga aacagtatat ccgccaaatg gcatccaatg
     6061 gctttgtcgg tattttcact tccatgcata taccagaaga tgatgtttcg gcatatcaaa
     6121 agcgattgat cgaactagga agcgaagcga aaaagaataa actagaattg atggtcgata
     6181 tttcaggaga ggcattggaa cgagcgggtt tttcattaga agatctaacg cctttgaaag
     6241 caattggtgt aacaggttta agaatggatt atcatatttc caatcagcag attgccgact
     6301 ggtcacatca attgaaaatc agtttgaatg ctagcacgat cacgcctaaa gatatcgatg
     6361 aattgaaaga agcagaagct gatttctcac agattgaagc ttggcataat tattatccac
     6421 gtcccgaaac agggctggac aaagaatggt atcagaaaaa aaatcaatgg ctcaaaattc
     6481 aaggtctcct tgtccaaggt tttgtaccag gagatacaga gttgagaggc ccactatatc
     6541 aaggattgcc aactttggaa gaacatcgag ggatccatcc tcttgcaggc gctctagatc
     6601 tattagcaag taacacggat attgtttaca tcggagatgc aggtctgtca gaaaacgttc
     6661 aggagcaatt tgcttctttc caaaaagaac agacagtctt gctccatacg gaacctgtag
     6721 atgaagaatt ttacgaatat atattaggga agcatacgaa tcggcaagac gatgcaagag
     6781 atgtcatacg aagcgtagat gcacgttttc gagagattcc gcctatccca gcaagaaata
     6841 cagctacgcg tatgaaaggg tctattactt tagataacga aaagtatttg cgctatatgg
     6901 gtgagatcca attaaccaaa tatgatttgc cagcggatga aaaagtgaat gtagtagcta
     6961 aagttatcaa agaagagcta ccattgatca accaaatcaa agctggtatg aactatcaat
     7021 ttataagaaa agaaggaaga taaaaatgag caaaatcaat ttagagaacc taacaacgga
     7081 acgcagaaac caagaaactt ttggattaga tgagatgagt atttctcaag ccgttcaact
     7141 aatgaacaaa gaagatcaga aagtggccgc tgcggttgaa aaagaactga caagtatcga
     7201 accagtgatt gctaacacga ttgagtcctt taaaaaaggt ggacgtctga tttatatggg
     7261 agccggaaca agtggaagac taggtgtgtt agatgctgct gaatgcgtac cgacattcgg
     7321 tgtagaaccg gaaatggtca taggtctaat tgctggtgga gaagaagcaa tgactgtagc
     7381 tgtcgaaggg gccgaagatt cttatgagct agggcaagaa gatctaatca agctgaattt
     7441 gacaaaaaat gatatagtta ttggtatcgc agctagcggt cgtacaccat atgtgatcgg
     7501 tggattagat tacgcaaaat cgatcggtgc tgtcacagga acgatttctt gcaataaaca
     7561 agccgaaatc agtaaacacg cggatttccc aatcgaagtc gatgcaggtc cagaattttt
     7621 gacaggctct actcgattga agtcaggaac agcacaaaaa cttatcttga atatgatttc
     7681 tactatcagt atgattggta taggtaaagt attcaataac ttgatggtcg atgtcaaacc
     7741 aacaaatgaa aaactaatcg aacgctcaaa acgaatcatc atgcaagcga cagaagctga
     7801 ttacgaaaca gctgaaaagt actttgaaga agcagatcaa aatgttaagc tggctatcgt
     7861 catgattttg acaaatagtg ataaagagga agctcaagca aaattagctg aagcaaacgg
     7921 atttgtaaaa gggaccttat gagggggaaa agaccatggc aaaaaacgaa ctgactttgg
     7981 aagaactggc aaaacaaatt tataaagggg caggcggcat gggcaatgta gaaagcattg
     8041 tccactgcat gactcgtgtg cgtatgaaga tcatcaatga tgatcttgta aatgtctctg
     8101 aattaaaagc gattccagga gtgcttggtg tagtagaaga tgaacagctt caagtcatca
     8161 tcggacctgg gaaagtgaac aaagtagcac agcaaatggt tgatttggca ggcgtgaaat
     8221 taggagaaaa actttcttct gtaagtacag ctacagcaaa ttcatcagca tctggaaaag
     8281 accgtgtaaa cgaacgggca caagaaatga aagcggcaca aaaatcgaaa caaaaaccat
     8341 ctaaaatcaa atcggtatta aaagacattt ctaatatctt tgtgccaatg attcctgcat
     8401 ttgtcggtac tgggatcgtg gcagggattg ccgcagtttt agcaaactta gtcacagccg
     8461 gaacacttga tgcagcaaca tggcaacaat atatcgatgt gatgaacatc ttgaaaaacg
     8521 ggatgtttag ctacctagtt atctatacag gtatcaatgc tgcacaagtt tttggtgcaa
     8581 caccaactct tggtggagtt atcggggccg ttgtgatgct gacagggatg aatccggaag
     8641 caccactgaa aaacttattt actggtgaag ctcttgcagc tggacaaggt gggatcctag
     8701 gtgtcatctt cgctgtttgg ctattgtcaa tcgttgagaa aaaactgcat aaagtagtac
     8761 ctgatgcggt agatatcatc gttacaccaa ctctttcatt gattgccatc ggtctgattg
     8821 aaattttctt catcatgcct ttagcaggtt tcatttctga tggcatggtc ggtgggatca
     8881 actgggtact tggcgttggt ggagcatttg ccggcttcgt tctaggcaca ttattcttgc
     8941 cgatggttat gttcggactg catcaaattt tgactccaat ccatgtgcaa atgattgatg
     9001 aaactggtcg tacgctattg cttccaattc ttgcgatggc tggtgcagga caagtgggtg
     9061 cagcattggc attatggatt cgctgcaaaa aagataaaga attaacagaa atgatcaaag
     9121 gagctttacc agtcgggatc ctaggaatcg gcgaaccatt gatttacgga gtaacattac
     9181 ctctaggacg cccatttatt actgcttgta tcggtggagg tatcggcggg gcagtgatcg
     9241 gaatgatcgg aaatgtcggg gctatcgcta ttggaccttc aggagccgct ttgatcccat
     9301 tgatctctga cggtaaatgg ttaggctaca tccttggatt attggcagct tatgctggtg
     9361 gcttcgttgc tacattcttc ttcggtattc ctaaagaccg tttagaaaaa gaagaattag
     9421 cgactgaaac agccacagta aatgaagtgc ctgaaaaagt agtacctcaa acttcagctg
     9481 aaagtgtgac tatcctctca tcagttgctg acggaacagt tgtttcttta gataaagcaa
     9541 gtgatccagt ttttgcacaa aagatgatgg gtgaaggcta ctttgtagaa ccagaaaacg
     9601 gacatatcta ttctccagtt actggggtag tcagctctgt cttcccaacg aaacacgcta
     9661 ttgggatcac aacgccaaat ggtttagaaa tcttgcttca tatgggaatc aacacagttg
     9721 acttaggcgg tacgccattt gatgtgaaag taacagaagg gcagcaagtg acacctgata
     9781 cgcttgtagc agaagctgac ttagaagcga tcaaagcagc aggcaaagaa acatccatga
     9841 tcgtcttggt aaccaatatg gatcgtgtaa aaaactttgt cttggaaaaa acaggtaaag
     9901 caaaagcaaa agcacctgtg atggatgtca aagcttaata tgatagaaac aatagaaacg
     9961 aaagaagaaa ctgaaatcta tttttcagct tcttcttttt tattatttat tgttttctgc
    10021 taaaatctga taaattcgtg tcacatcaga tggtgttccc cgtaattcat aatctgccaa
    10081 gaaaggaaag ccaaatgttt gtgcatattg tttggctgtt agacaatact gattattgaa
    10141 gtttttattt ccgctaccga ctacaccaag acaaagttta tgattgtctt catgttccag
    10201 atattcacgc atcgtttcgg tcaagatttc tgtatcaccg ttatctaaac catttccgcc
    10261 gtctaaataa gtaggaacga aagtaaaaaa tggttctgtt tctttcgtaa aatcactatt
    10321 ttcatggatt tcttttaaag taacttcagg attgacagga tcttctgca
//
LOCUS       DAIGOK010000317         5099 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.guided.140, whole genome shotgun sequence.
ACCESSION   DAIGOK010000317 DAIGOK010000000
VERSION     DAIGOK010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.guided.140"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            388..471
                     /locus_tag="NOA01_002811"
     CDS             388..471
                     /locus_tag="NOA01_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HCI0633911.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            596..1333
                     /gene="erm(B)"
                     /locus_tag="NOA01_002812"
     CDS             596..1333
                     /gene="erm(B)"
                     /locus_tag="NOA01_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HCI0633912.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1596..1712)
                     /locus_tag="NOA01_002813"
     CDS             complement(1596..1712)
                     /locus_tag="NOA01_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta-toxin"
                     /protein_id="HCI0633913.1"
                     /translation="MVQNQHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI"
     gene            1975..2487
                     /locus_tag="NOA01_002814"
                     /pseudo
     CDS             1975..2487
                     /locus_tag="NOA01_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337518.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(2559..4172)
                     /locus_tag="NOA01_002815"
     CDS             complement(2559..4172)
                     /locus_tag="NOA01_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HCI0633914.1"
                     /translation="MNEKFARWNVLFIQYVKRDWKKIIVWVLGLGLFSGAFVPAFEEI
                     AKGQGLLGMFETMQNPAMISMVGPTPIKIGTDYTLGAMYAQEMLLFCGLFAMIISALH
                     VVSHTRKEEELGLTELVRSFRVGRQANSLAVISEMLLINLLLGLLIGGLMMSFGVKTI
                     DAEGAFLFGGSIALAGIIGGVLALVMSQIMATSTGATGSTLSLIGLLYIVRAGTDVSN
                     LDLSMFNPMGWIYLTYPFTENNWLPLLFALIFSLVFTVLAFVLEEHRDMGAGYLPERE
                     GRATAKKSLLSVPGLFFKINKGVMIGWLIAFVVMGAAYGSIYGDMQVFLGGNELMKQM
                     FTQSGVSIEESFTATIMMVMIGLVTILPIAVVNKLFAEETRLHLSQLYVTKITRGQLY
                     WTTIFLAIFAGVVGIGLASAGLGGTAISAMKNESTMDLTDFLAAGYNFLPSILFYIGL
                     AALALGWLPKFGKVIYAYLGYSFALNYFGGILDLPDWFSKTAIQSWIPRLPMEKFDGT
                     IFAVITVISIVFLFVGYLGYKRRDMVEGA"
     gene            complement(4196..5050)
                     /locus_tag="NOA01_002816"
     CDS             complement(4196..5050)
                     /locus_tag="NOA01_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCI0633915.1"
                     /translation="MQKKFGKFQALKDVSFTVNAGEVVGFIGPNGAGKSTTIRTLLGI
                     INRDEGDVQIFGKDVWKDSLEIHKRISYVPGDVALWGSLTGGEIIDLFIKLHGGGSKA
                     KRDYLIKRFELDPKKKAKGYSKGNRQKVGLIAALSVESDLYILDEPTSGLDPLMEAVF
                     QEEVEKIKNDGKAILLSSHILSEVERLADKVAIIRRGEVVETGTLDELRHLTRSTVTL
                     VTEGDIEKLATLSGVHDFVQKDGKATFSADNEAMNTILTEATKLGVTKIESVPPTLED
                     LFMRHYEG"
BASE COUNT         1639 a         1005 c          880 g         1575 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaacct tttgtacatt taacttgacg
       61 gtgacatctc tctattgtga gttattagtg gtacagtttt caaccgtttt aattataaaa
      121 aagtggtgca tttttaaatt ggcacaaaca ggtaacggtt attgcaggtg tatttcttat
      181 ctatgggttt aacatggatt ttatcattaa aatcatgagt attgtccgag agtgattggt
      241 cttgcgtatg gttaacccta aagttatgga aataagactt agaagcaaac ttaagagtgt
      301 gttgatagtg cattaaaaat tttgtataat aggaattgaa gttaaattag atgctaaaaa
      361 tttgtaatta agaaggaggg attcgtcatg ttggtattcc aaatgcgtta tgtagataaa
      421 acatctactg ttttgaaaca gactaaaaac agtgattacg cagataaata aatacgttag
      481 attaattcct accagtgact aatcttatga ctttttaaac agataactaa aattacaaac
      541 aaatcgttta acttctgtat ttgtttatag atgtaatcac ttcaggagtg attacatgaa
      601 caaaaatata aaatattctc aaaacttttt aacgagtgaa aaagtactca accaaataat
      661 aaaacaattg aatttaaaag aaaccgatac cgtttacgaa attggaacag gtaaagggca
      721 tttaacgacg aaactggcta aaataagtaa acaggtaacg tctattgaat tagacagtca
      781 tctattcaac ttatcgtcag aaaaattaaa actgaatact cgtgtcactt taattcacca
      841 agatattcta cagtttcaat tccctaacaa acagaggtat aaaattgttg ggagtattcc
      901 ttaccattta agcacacaaa ttattaaaaa agtggttttt gaaagccatg cgtctgacat
      961 ctatctgatt gttgaagaag gattctacaa gcgtaccttg gatattcacc gaacactagg
     1021 gttgctcttg cacactcaag tctcgattca gcaattgctt aagctgccag cggaatgctt
     1081 tcatcctaaa ccaaaagtaa acagtgtctt aataaaactt acccgccata ccacagatgt
     1141 tccagataaa tattggaagc tatatacgta ctttgtttca aaatgggtca atcgagaata
     1201 tcgtcaactg tttactaaaa atcagtttca tcaagcaatg aaacacgcca aagtaaacaa
     1261 tttaagtacc gttacttatg agcaagtatt gtctattttt aatagttatc tattatttaa
     1321 cgggaggaaa taattctatg agtcgctttt gtaaatttgg aaagttacac gttactaaag
     1381 ggaatgtaga taaattatta ggtatagggc acctctaata actaccaatt aattcacctc
     1441 aaaatgaaat tagaaaaaaa cagcgaaatt tttaaatcta tttcttatcg atacaaattc
     1501 ctcgtaggcg ctcgggaccc caacatgagg aaatgttttt ataaaaaaag agtagttacc
     1561 gttttttggt aactactctt ttcctttttt aaggtttaaa tacctggaag tttaggtgtt
     1621 ttgggtattg gtggtgtagg tggctgcaag ctttccaatt tttgttgaat tgctttaaat
     1681 tcaggggttt cttggtgttg attttgaacc attttttgct ctattctctc caaagtcggt
     1741 tgaatttctt tccctgatac tttacgattt aattctcttt ctaaggtctc tttcggacta
     1801 atggaaggcg tttctaagct tgaatagagt ttcactcctt ctctattgta aagccttata
     1861 tcgctaaata agcccgtttt atgaagggtt tctaaattgg tcggtaagtt tttgacaaca
     1921 atatcatgcg cttgttttgg tggtgcccta gctgtcattg gatcatctgc atacaataag
     1981 atccatgaaa ggccatctgc tgccaatgag cgccttgaga ttggtaattg ggaagctgat
     2041 actgtagccg gtaaaacagg tagggctcgt attgtaactc tagtcgatcg tagatctcgt
     2101 ttctccctac ttggtaaggt tgaaaagaaa atctctaaaa atgtggttga ttgtatgatc
     2161 aatctactaa gaaatgtagg agaagataaa tgtaaaaccg ttacacccga tagaggaaaa
     2221 gaattctccc aacatagtcg aataacgcta gaattaaatg gcactaaagt gtattttcca
     2281 gatccacatg ctccatggca acgtggtaca aacgaaaata ctaatggttt attacgagaa
     2341 tattcaccca aaggtgaaga tatttcaggt attttcgata gtcaaattca agaatgggcg
     2401 aataaattaa acacgcgacc acggaaatgt ctaaattgga aaacccctta tgaagtattt
     2461 tttaatgaat cgttgcactt aatttgacaa ttgaagccat aaaaaaaccg ctcaaatcga
     2521 ctttcgtaaa tatgaacggt tttttagaaa ttcaattttt aagcgccttc taccatatca
     2581 cggcgtttgt atcctaaata gccgacaaat aagaagacga tactgataac agtaattact
     2641 gcaaaaatcg ttccatcaaa tttttccatc ggtaagcgtg gaatccaact ttgaattgca
     2701 gtttttgaga accaatccgg caaatctaag attccgccga aataattcaa agcaaaggaa
     2761 tagcctagat aagcatagat tacttttcca aattttggca accagcctaa cgctaaagca
     2821 gccaaaccaa tataaaataa gatggaaggg agaaaattgt atccagcagc taagaaatcg
     2881 gtcagatcca tagtcgattc atttttcatc gcagaaatcg ccgttccacc taatcccgct
     2941 gatgctaagc caatacctac gactccagca aaaatagcta aaaatatcgt tgtccaatat
     3001 aattggcctc gcgtaatctt cgttacatac agttgactca gatgcagtct tgtttcttct
     3061 gcaaataatt tattgaccac cgcgattggc aagattgtga ctaatccaat cattaccatc
     3121 atgatcgttg ccgtaaagga ttcttcaatg gaaacgccag attgagtgaa catttgtttc
     3181 atcagttcat ttccgccaag aaagacttgc atgtctccat aaatggagcc atacgcagct
     3241 cccataacga caaatgcgat cagccaacca atcattactc ctttattaat cttgaaaaac
     3301 aaaccaggta cagaaagtag tgatttcttc gccgtcgcac gtccttctcg ttcaggaaga
     3361 taacctgcgc ccatgtcgcg atgttcttcc aacacaaacg caagtacggt aaaaacaaga
     3421 ctaaaaatca aagcaaataa taatggtagc cagttatttt ctgtgaaagg ataggtcaag
     3481 taaatccatc ccattggatt gaacattgat aaatcaagat tagacacatc tgttccagcg
     3541 cgcacgatat acaaaagtcc tataagactt aatgtcgagc cggttgctcc agtagaagtc
     3601 gccataatct gcgacatcac aagtgccaat acaccaccga taattcccgc caatgcaatt
     3661 gatcctccga acaagaaagc tccttcggca tcaatcgttt ttacaccaaa actcatcatg
     3721 agtccgccga ttaaaagacc taataaaaga ttgatcaaca gcatctcact gataacagct
     3781 aatgaattgg cttgtcgtcc tactcgaaat gagcgaacca attcagtcaa acctaattct
     3841 tcttcttttc gcgtgtggct caccacatga agtgctgaga taatcattgc gaacaatccg
     3901 caaaacagca acatctcttg agcatacatc gctcctaaag tataatccgt acctattttg
     3961 attggtgtag gtccaaccat cgagatcatc gctggatttt gcatcgtttc aaacatccct
     4021 aaaagacctt gtcctttagc aatctcttca aatgctggta caaatgctcc tgagaacaaa
     4081 cccaaaccta aaacccaaac aattattttt ttccaatcgc gtttcacgta ttgaatgaac
     4141 aatacgttcc aacgggcaaa tttttcattc attttctttt cctccgttcc gacaatcagc
     4201 cttcgtaatg acgcatgaat agatcttcaa gcgttggcgg tacagattcg atttttgtca
     4261 cacctaattt ggttgcctcg gtcagaatcg tattcatcgc ttcattgtca gcagaaaaag
     4321 ttgctttgcc gtctttttga acaaaatcat gcacgccaga gagcgtcgca agtttctcaa
     4381 tatcgccttc tgtcaccaat gtaactgttg agcgagtcaa atgacgcaat tcatctaatg
     4441 tacctgtttc aactacttct ccacgtcgaa tgattgctac tttatctgct aatcgttcaa
     4501 cttcacttaa aatatgtgaa gataatagaa tcgctttgcc atcatttttg attttttcta
     4561 cttcttcttg gaatactgct tccatcaatg gatctagtcc tgaagtcggt tcatctaaaa
     4621 tatacagatc agattcaact gaaagtgcag caatcaaacc gactttttga cgatttcctt
     4681 tagagtaacc tttggctttt ttctttggat caagttcaaa tcgtttgatt aaataatcgc
     4741 gctttgcttt gctcccgccg ccatgaagtt tgataaatag atcaatgatc tctccaccag
     4801 tcaggctgcc ccaaagagca acatctccag gaacatacga aattcgttta tggatttcta
     4861 gactatcttt ccaaacatct tttccgaata tttggacatc tccttcgtct ctgttgatga
     4921 ttcctagcag tgtacggatc gtcgttgact ttcctgctcc atttggtccg ataaaaccaa
     4981 caacttcacc ggcgtttact gtgaatgaga catctttcaa cgcctggaat ttaccaaatt
     5041 ttttttgcaa gccttgtact ggttctgttg caaagtttta aataaagaat aaaatccct
//
LOCUS       DAIGOK010000318         4523 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.guided.156, whole genome shotgun sequence.
ACCESSION   DAIGOK010000318 DAIGOK010000000
VERSION     DAIGOK010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.guided.156"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            22..933
                     /gene="vanY"
                     /locus_tag="NOA01_002817"
     CDS             22..933
                     /gene="vanY"
                     /locus_tag="NOA01_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HCI0633916.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYELWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1086..1571
                     /gene="vanZ"
                     /locus_tag="NOA01_002818"
     CDS             1086..1571
                     /gene="vanZ"
                     /locus_tag="NOA01_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HCI0633917.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(1858..2187)
                     /locus_tag="NOA01_002819"
     CDS             complement(1858..2187)
                     /locus_tag="NOA01_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCI0633918.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            2492..2821
                     /locus_tag="NOA01_002820"
     CDS             2492..2821
                     /locus_tag="NOA01_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCI0633919.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2811..3098
                     /locus_tag="NOA01_002821"
     CDS             2811..3098
                     /locus_tag="NOA01_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCI0633920.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(3377..3940)
                     /locus_tag="NOA01_002822"
     CDS             complement(3377..3940)
                     /locus_tag="NOA01_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCI0633921.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT         1595 a          672 c          761 g         1495 t
ORIGIN      
        1 tagttaagga ggagactgaa aatgaagaag ttgttttttt tattgttatt gttattctta
       61 atatacttag gttatgacta cgttaatgaa gcactgtttt ctcaggaaaa agtcgaattt
      121 caaaattatg atcaaaatcc caaagaacat ttagaaaata gtgggacttc tgaaaatacc
      181 caagagaaaa caattacaga agaacaggtt tatcaaggaa atctgctatt aatcaatagt
      241 aaatatcctg ttcgccaaga aagtgtgaag tcagatatcg tgaatttatc taaacatgac
      301 gaattaataa atggatacgg gttgcttgat agtaatattt atatgtcaaa agaaatagca
      361 caaaaatttt cagagatggt caatgatgct gtaaagggtg gcgttagtca ttttattatt
      421 aatagtggct atcgagactt tgatgagcaa agtgtgcttt accaagaaat gggggctgag
      481 tatgccttac cagcaggtta tagtgagcat aattcaggtt tatcactaga tgtaggatca
      541 agcttgacga aaatggaacg agcccctgaa ggaaagtgga tagaagaaaa tgcttggaaa
      601 tacgggttca ttttacgtta tccagaggac aaaacagagt taacaggaat tcaatatgaa
      661 ctatggcata ttcgctatgt tggtttacca catagtgcga ttatgaaaga aaagaatttc
      721 gttctcgagg aatatatgga ttacctaaaa gaagaaaaaa ccatttctgt tagtgtaaat
      781 ggggaaaaat atgagatctt ttattatcct gttactaaaa ataccaccat tcatgtgccg
      841 actaatcttc gttatgagat atcaggaaac aatatagacg gtgtaattgt gacagtgttt
      901 cccggatcaa cacatactaa ttcaaggagg taaggatggc ggaatgaaac caacgaaatt
      961 aatgaacagc attattgtac tagcactttt ggggtaacgt tagcttttta atttaaaacc
     1021 cacgttaact aggacattgc tatactaatg atacaactta aacaaaagaa ttagaggaaa
     1081 ttatattggg aaaaatatta tctagaggat tgctagcttt atatttagtg acactaatct
     1141 ggttagtgtt attcaaatta caatacaata ttttatcagt atttaattat catcaaagaa
     1201 gtcttaactt gactccattt actgctactg ggaatttcag agagatgata gataatgtta
     1261 taatctttat tccatttggc ttgcttttga atgtcaattt taaagaaatc ggatttttac
     1321 ctaagtttgc ttttgtactg gttttaagtc ttacttttga aataattcaa tttatcttcg
     1381 ctattggagc gacagacata acagatgtaa ttacaaatac tgttggaggc tttcttggac
     1441 tgaaattata tggtttaagc aataagcata tgaatcaaaa aaaattagac agagttatta
     1501 tttttgtagg tatacttttg ctcgtattat tgctcgttta ccgtacccat ttaagaataa
     1561 attacgtgta agatgtctaa atcaagcaat ctgatctttc atacacataa agatattgaa
     1621 tgaattggat tagatggaaa acgggatgtg gggaaactcg cccgtaggtg tgaagtgagg
     1681 ggaaaaccgg tgataaagta aaaagcttac ctaacactat agtaacaaag aaagcccaat
     1741 tatcaatttt agtgctgagg aattggtctc tttaataaat ttccttaacg ttgtaaatcc
     1801 gcattttcct gacggtaccc ccaaactaag atttcataga tagattttct gataacttta
     1861 aaaaacacga atagcggaaa ggatgtcttc attcgctaaa gagtaataaa ctacttttcc
     1921 atcttgacgt gattttgcca ttccatgttt ttttaaaaaa cgtaaatggt gtgacgttgt
     1981 agcgacacta gcagtaagta tttctgctaa atcacagacg cacatttcct tataggtttc
     2041 taaagcatag aaaattttaa ttctagaaaa atcgctgaaa catttcccta acacaagtaa
     2101 gtttgaaaaa tctttttgat ccaatttatt ttttattcca tttacttttt ctttatgtac
     2161 gacagtcact tggcaaactt ttttcattca tctcacctca cattcaaacg attatttgaa
     2221 tgaagtatac ctatttgatc actcttttgt caatctacct taattccaat acactattat
     2281 aagcaatttc atgtattgaa gtaaacttac ttgcaagtgc gtttttgatt ccaagttaaa
     2341 aacatattat tattctacat aatttcctaa tcaaataaaa ctcctttgaa aattgttcta
     2401 aaggagtttt attattttgt atttctacta aaaatagcgt acaatgtaat tactaagtaa
     2461 ttacgaaatg taaaataatg gaggaaataa tatgattaca aaaacaagaa aacaaggaaa
     2521 ttctattatg ttaacggtcc ctaaagaatt cgatgtgccc aatggtgttg aagtagaagc
     2581 taagcttgta gagaatggta tcctttatga gtttgtagag cctaaaaaag aattttttga
     2641 ttttagtgaa gatattttgg ctgatattct ttcagaaggt tatagcaaac aagagatctt
     2701 aaaagaattt agaaatagaa aaaatgcgtt aacttctgca tttaaatcta ttgctgaaga
     2761 tactgttgca aattctaaac cgatgacaaa agaggagttg gcagcagaaa ttggcttatg
     2821 agattctacc ttctaaacac gtaataaagt atctaaaaaa attaaaagag aaaacattaa
     2881 aagaacaatt tttaacgata atttatgatg agatagcagt tcgtcctcat tcgggagaac
     2941 aaaaaacggg tgatttatca ggaatttggg ctattggatt caagtatgca ggtacgacat
     3001 atcgcgttgc atatgaaatt aaagataatg cagtgatacc tatcttacta tgtggaacgc
     3061 atgaaaattt ttatgaacaa ttaaaaaaga ttaggtaaga acccaatgtt tcaacttgag
     3121 aggatagacg acactttaaa agataatttt taacagctga gagttgtaat accctataat
     3181 tttaaaccac taacctatat atttatgaaa aatagaaaaa attaaaacgc attttaagcc
     3241 tctttcaaat taaaagcttc atccttatgc acaaagaggg cttaaaatgc gttattttat
     3301 attgtttttt agtaataata aaaaaatcac tctaattctc ccataaatat tatagattcc
     3361 taaagattaa aatagattat tccctattgt cttttaagta gcggtagagt gttgccttgc
     3421 taataccagt catttccgta atttctttta cgctataaac ctgactttta tacaatttta
     3481 ctgctcgatc aacatctttc tggttcactc gtggacggcc acctttacga cctctcgcac
     3541 gtgcactttt caaaccttct ttggtacgtt caacaattaa atcacgttcg aactgactaa
     3601 atgcttggaa gacagtcatc attaagcgtc cgtgaggcgt gctggtatcg aagttttctt
     3661 tcaagctcac cagcttaaca tggtgttctt caaaataggt aacaaggtca atcaaatcct
     3721 ttgtactacg acccaatcga gaaaaacttt ctacaattac cgtatctccc ggacgaattt
     3781 tatcttttaa acgatttagt tggggacgaa ccgctttggt tcccgtcatt ttctcagtta
     3841 ggatttcatt acaattttgt tcattgagta agtctaattg acgatttagt tcttgtgaac
     3901 gtgtactcac tcgggcataa ccataaatgt atccactcat gcttttcttc ctttcgtact
     3961 ttaattctgg cttgattgta tcataaaagg gagttataga tacatagtta atgcaacaag
     4021 ttatgataca aaatatgacc tcttttattt aataaaagag gtcatttcat tttgtctcat
     4081 aatcgattgt ttttgatacg agtgtgtata cgaaataaac aaaaaaatct taagtaaatt
     4141 tctagatacc taatttaatc ctaactagtg tatcctaaag ttaataacga gtttatcaca
     4201 atgaaaatta taaaatgctc ccatattaga tataacatgc aatatttgaa ttagaactac
     4261 aaaatttatt cgttttataa agtaaataat gttgagatac taaggaatag aatattataa
     4321 ggaattgagc tgaatgtatt ggataaaaaa agaataactt tattagagat ggactataaa
     4381 aatgctcgta attttcttct gaagtctgaa agctatttga atgctcagct accagaatat
     4441 attagttttg acgaaacttt aaaatctgct aaaaaagttt taacttcgcc gtttgtataa
     4501 ttaagcgaga caaataaaaa agc
//
LOCUS       DAIGOK010000319         3368 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.guided.184, whole genome shotgun sequence.
ACCESSION   DAIGOK010000319 DAIGOK010000000
VERSION     DAIGOK010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.guided.184"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..152
                     /locus_tag="NOA01_002823"
     CDS             <1..152
                     /locus_tag="NOA01_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCI0633922.1"
                     /translation="DHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNN
                     NGTKK"
     gene            136..765
                     /locus_tag="NOA01_002824"
     CDS             136..765
                     /locus_tag="NOA01_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCI0633923.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            1119..2597
                     /gene="msr(C)"
                     /locus_tag="NOA01_002825"
     CDS             1119..2597
                     /gene="msr(C)"
                     /locus_tag="NOA01_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HCI0633924.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            2644..3090
                     /locus_tag="NOA01_002826"
     CDS             2644..3090
                     /locus_tag="NOA01_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HCI0633925.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(3289..>3368)
                     /locus_tag="NOA01_002827"
     CDS             complement(3289..>3368)
                     /locus_tag="NOA01_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HCI0633926.1"
                     /translation="RRIMLCQQELQWCQQVREQMQQFFE"
BASE COUNT         1121 a          562 c          707 g          978 t
ORIGIN      
        1 tggaccataa agatacaggt tccgtgctat ccccggcttt cagcgatgta gaaatgaaac
       61 aatttctaac acttttagat aaattgaata cccaactttg caatataaca acagaaacga
      121 aagaggttaa taataatgga acaaaaaaat gatttttcaa caattatgac tggtagaaga
      181 tccgttcgcg tatatgatga aacatacaaa atcccacacg aagaaatgct ggaaatgatc
      241 aaagaagcga cgactgcacc ttcttctgtc aatatgcaac catggcgctt tgtggtcgct
      301 gaaagcgagg aagcaaaaga aatcttgcgt cctttgatcc gtttcaatac gagacaaaat
      361 gatacttctt ctgcaatggt catgattttt ggcgatatgc aatgttacga atatggcgaa
      421 gaaatctata accaagctta cgaatcaggg aagatgccaa aagaagtcag agatcaacaa
      481 ttagctgcga tcattcctta ttacaagagt ttttcaagag aagaaatgaa cgatgtagtc
      541 aaagtcgatt ctagcttagc ggctatgcaa ttcatgttag ttgcgcgtga tcatggctat
      601 gaaacaaatc caattggtgg attcgaagca gatcagttgg cagaggcatt tggtttggat
      661 aaagaccgtt atgttccagt tattatttta tcaataggaa aagcggtaga agaaggttac
      721 gagtctgttc gtttagcacc agaaaagatc actacgttcg aataaaaggt tttcagttac
      781 tattcagtag ctattgcttt ttttgaatcg tgtgtggtaa cctaataaaa aaggaggtcg
      841 gaaaaatgac tgcatcgatg aaattacgtt tcgaactttt gaataacaac taaataaatt
      901 caaaagacat gtataaacaa caatttcatg gatggagtat gtccattttt tcgaacgaca
      961 gtagaatagg tgaaagctat gacagatttg gtgatttagt catagttatt tcgttcagag
     1021 taggaaaaga tgtgatgcag tttttttgca aacagatttt tctgctcttt tttagtatcg
     1081 ataattgttg ttttacatga aataggagtg taaaacatat ggaaaattta gcagtaaata
     1141 taacaaatct acaagtcagc tttggcaacc agctagaact atctattgat tctcttcgtg
     1201 tctatcagca agatcgtata gggatcatag gagaaaatgg tgtcggtaaa tcgactttgc
     1261 tcaaactaat agccggtgaa ctttttcccg atcatgggaa aatccaaaca gagatcacct
     1321 tcaactacct gcctcaatta acctatctcg ctgaagcaaa ggacctaaat ttggaattag
     1381 ccagtcattt ccagttaaga ctggaagaaa cttcagagcg gaaatggagc ggaggggaag
     1441 aacgaaagat cgagttgata cgtcttcttt cttcttatga acaagggatg cttctagacg
     1501 agccaacaac ccatctagat agaaaaagta ttgatcgact gattgaagag cttcgttatt
     1561 attatggtac gctggttttt gttagtcatg atcgctattt tctagatgag ctagcatcga
     1621 aaatctggga agtaaaagac ggagaaatcc gagagttttc ggggaattat agtgcctatc
     1681 tcactcaaaa ggaattggag aaaaagactc agctacgaga agcagagtcg atcatgaaag
     1741 agaaaaaacg attggaaaaa tcgatccaag aaaagaaaaa acaagcggaa aagttggaaa
     1801 aagtgtccag taaaaagaaa aagcaacaaa tcagaccgga tcggttgtct tcctctaaac
     1861 aaaaagacag tgtacaaaaa gccatccaaa agaatgcgaa aacattagag agaagactcc
     1921 aaaaaatagg agaaacaacc aagccgcagc agatgaaaca aatccgtttt ccagtaccaa
     1981 aatctcttga actccacagc cgctatccca tcatgggaca aaatgtccaa ttggaaagaa
     2041 gtgggagaac attactggta aatggtgatt ttcagttttc tttaggtaaa aaaatcgcga
     2101 ttgtcggtga aaatggttca ggtaagacaa ccttattaga acatatccgc aaacaaggag
     2161 aaggaatcct tctctctccg aaagtaagct ttcaagtata tcagcaaaag ggttatcaaa
     2221 tgacatctga agaatccatc attcgttttg tcatgagaca aacagagttt tcagaatcgc
     2281 ttgtccgtag tttgctgaat cacttagggt ttgctcagga aactttgacg aaaccgttat
     2341 gtacattaag tgggggagaa gcgacccgtc tgacgattgc tttgcttttt actaagccaa
     2401 gtaatgtgtt gctgttagat gaaccgacta attttattga tatggcaacg atcgaagctt
     2461 tagagaagct aatgcaaata tatccgggaa cgattttgtt tacttctcat gattcttact
     2521 ttgtcgagcg tacggctgat gaagtttatg aaataaaagg gcagaaaata aaaaaagtac
     2581 ttacgagaaa tttttaaatg tgctacgatg attttcgtga caaatttaaa ggagtggtac
     2641 agattgccta caaataaaaa agaaggaata atttttacaa caatgatgtg ttttttgatg
     2701 gtcttgggga tgagcgcgta taatttatgg ctgcatcaag atcttcattt atcaagtttg
     2761 ttgattggtc tagtgcctgg attcgtggtg gctttcattt tggatgtctt catcatcggt
     2821 gtgctggcga aaaaaattgc ctttaaacta ccagtaaatc cagaaagcaa ggtccaaatg
     2881 atcttaacta tttcatgctt gatggtttta ggaatggtga catgtatgtc tttattcggt
     2941 gtgatcatgg aacaaggaat acctgatcaa ttattctctg cttatatgaa tgcttggaaa
     3001 atgaatgtga ttgccgcttt accgttgcag ttgatcatcg ttggcccgct ttcaagaagt
     3061 gtgctaaaag caattcaaag taatgaatga tggaacactg ttatttgaaa aaaggccttg
     3121 gcatgctttt tgtcatagtc tcttgaatgc agttctagaa aaatagtgat ccgaacgata
     3181 ggttttggcc tgtcgttcgg atcgcttttt cttttccaac tgtattgctc ttttattgaa
     3241 gataatttgc tcttcaatag atatttctag aaaggttctg tttatccctt actcaaaaaa
     3301 ctgctgcatt tgttctctta cttgctgaca ccattggagt tcctgctgac aaagcataat
     3361 tctgcgct
//
LOCUS       DAIGOK010000320         3297 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.guided.186, whole genome shotgun sequence.
ACCESSION   DAIGOK010000320 DAIGOK010000000
VERSION     DAIGOK010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.guided.186"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            245..1213
                     /locus_tag="NOA01_002828"
     CDS             245..1213
                     /locus_tag="NOA01_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HCI0633927.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1206..2237
                     /gene="vanA"
                     /locus_tag="NOA01_002829"
     CDS             1206..2237
                     /gene="vanA"
                     /locus_tag="NOA01_002829"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HCI0633928.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2243..2851
                     /locus_tag="NOA01_002830"
     CDS             2243..2851
                     /locus_tag="NOA01_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HCI0633929.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          985 a          626 c          796 g          890 t
ORIGIN      
        1 atgctggtgt catccaccaa catcaaaaag tatagagcca taatttttta ggaaaatctc
       61 aaggttatct ttactttttc ttaggaaatt aacaatttaa tattaagaaa cggctcgttc
      121 ttacacggta gacttaatac cgtaagaacg agccgttttc gttcttcaga gaaagatttg
      181 acaagattac cattggcatc cccgttttat ttggtgcctt tcacagaaag ggttggtctt
      241 aattatgaat aacatcggca ttactgttta tggatgtgag caggatgagg cagatgcatt
      301 ccatgctctt tcgcctcgct ttggcgttat ggcaacgata attaacgcca acgtgtcgga
      361 atccaacgcc aaatccgcgc ctttcaatca atgtatcagt gtgggacata aatcagagat
      421 ttccgcctct attcttcttg cgctgaagag agccggtgtg aaatatattt ctacccgaag
      481 catcggctgc aatcatatag atacaactgc tgctaagaga atgggcatca ctgtcgacaa
      541 tgtggcgtac tcgccggata gcgttgccga ttatactatg atgctaattc ttatggcagt
      601 acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat gatttcaggt tggacagcga
      661 ccgtggcaag gtactcagcg acatgacagt tggtgtggtg ggaacgggcc agataggcaa
      721 agcggttatt gagcggctgc gaggatttgg atgtaaagtg ttggcttata gtcgcagccg
      781 aagtatagag gtaaactatg taccgtttga tgagttgctg caaaatagcg atatcgttac
      841 gcttcatgtg ccgctcaata cggatacgca ctatattatc agccacgaac aaatacagag
      901 aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt ccacttgtag atacctatga
      961 gttggttaaa gcattagaaa acgggaaact gggcggtgcc gcattggatg tattggaagg
     1021 agaggaagag tttttctact ctgattgcac ccaaaaacca attgataatc aatttttact
     1081 taaacttcaa agaatgccta acgtgataat cacaccgcat acggcctatt ataccgagca
     1141 agcgttgcgt gataccgttg aaaaaaccat taaaaactgt ttggattttg aaaggagaca
     1201 ggagcatgaa tagaataaaa gttgcaatac tgtttggggg ttgctcagag gagcatgacg
     1261 tatcggtaaa atctgcaata gagatagccg ctaacattaa taaagaaaaa tacgagccgt
     1321 tatacattgg aattacgaaa tctggtgtat ggaaaatgtg cgaaaaacct tgcgcggaat
     1381 gggaaaacga caattgctat tcagctgtac tctcgccgga taaaaaaatg cacggattac
     1441 ttgttaaaaa gaaccatgaa tatgaaatca accatgttga tgtagcattt tcagctttgc
     1501 atggcaagtc aggtgaagat ggatccatac aaggtctgtt tgaattgtcc ggtatccctt
     1561 ttgtaggctg cgatattcaa agctcagcaa tttgtatgga caaatcgttg acatacatcg
     1621 ttgcgaaaaa tgctgggata gctactcccg ccttttgggt tattaataaa gatgataggc
     1681 cggtggcagc tacgtttacc tatcctgttt ttgttaagcc ggcgcgttca ggctcatcct
     1741 tcggtgtgaa aaaagtcaat agcgcggacg aattggacta cgcaattgaa tcggcaagac
     1801 aatatgacag caaaatctta attgagcagg ctgtttcggg ctgtgaggtc ggttgtgcgg
     1861 tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga ccaaatcagg ctgcagtacg
     1921 gaatctttcg tattcatcag gaagtcgagc cggaaaaagg ctctgaaaac gcagttataa
     1981 ccgttcccgc agacctttca gcagaggagc gaggacggat acaggaaacg gcaaaaaaaa
     2041 tatataaagc gctcggctgt agaggtctag cccgtgtgga tatgttttta caagataacg
     2101 gccgcattgt actgaacgaa gtcaatactc tgcccggttt cacgtcatac agtcgttatc
     2161 cccgtatgat ggccgctgca ggtattgcac ttcccgaact gattgaccgc ttgatcgtat
     2221 tagcgttaaa ggggtgataa gcatggaaat aggatttact tttttagatg aaatagtaca
     2281 cggtgttcgt tgggacgcta aatatgccac ttgggataat ttcaccggaa aaccggttga
     2341 cggttatgaa gtaaatcgca ttgtagggac atacgagttg gctgaatcgc ttttgaaggc
     2401 aaaagaactg gctgctaccc aagggtacgg attgcttcta tgggacggtt accgtcctaa
     2461 tcgtgctgta aactgtttta tgcaatgggc tgcacagccg gaaaataacc tgacaaagga
     2521 aagttattat cccaatattg accgaactga gatgatttca aaaggatacg tggcttcaaa
     2581 atcaagccat agccgcggca gtgccattga tcttacgctt tatcgattag acacgggtga
     2641 gcttgtacca atggggagcc gatttgattt tatggatgaa cgctctcatc atgcggcaaa
     2701 tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg cgctccatca tggaaaacag
     2761 tgggtttgaa gcatatagcc tcgaatggtg gcactatgta ttaagagacg aaccataccc
     2821 caatagctat tttgatttcc ccgttaaata aacttttaac cgttgcacgg acaaactata
     2881 taagctaact ctttcggcag gaaacccgac gtatgtaact ggttcttagg gaatttatat
     2941 atagtagata gtattgaaga tgtaaggcag agcgatattg cggtcattat ctgcgtgcgc
     3001 tgcggcaaga tagcctgata ataagactga tcgcatagag gggtggtatt tcacaccgcc
     3061 cattgtcaac aggcagttca gcctcgttaa attcagcatg ggtatcactt atgaaaattc
     3121 atctacattg gtgataatag taaatccagt agggcgaaat aattgactgt aatttacggg
     3181 gcaaaacggc acaatctcaa acgagattgt gccgtttaag gggaagattc tagaaatatt
     3241 tcatacttcc aactatatag ttaaggagga gactgaaaat gaagaagttg ttttttt
//
LOCUS       DAIGOK010000321         2211 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.guided.208, whole genome shotgun sequence.
ACCESSION   DAIGOK010000321 DAIGOK010000000
VERSION     DAIGOK010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.guided.208"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..117
                     /locus_tag="NOA01_002831"
     CDS             <1..117
                     /locus_tag="NOA01_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCI0633930.1"
                     /translation="TLFDSRFKTLQQNPVNQICEITNVSRASLYRKLSEVNN"
     gene            331..1026
                     /gene="vanR"
                     /locus_tag="NOA01_002832"
     CDS             331..1026
                     /gene="vanR"
                     /locus_tag="NOA01_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HCI0633931.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1004..2158
                     /locus_tag="NOA01_002833"
     CDS             1004..2158
                     /locus_tag="NOA01_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HCI0633932.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          741 a          409 c          467 g          594 t
ORIGIN      
        1 accttatttg atagtagatt taaaactttg caacagaacc ctgtaaatca aatttgtgaa
       61 attactaatg tatctagggc ttcattatac aggaaattat cagaagtgaa taattagcca
      121 ttctgtattc cgctaatggg caatattttt aaagaagaaa aggaaactat aaaatattaa
      181 cagcctccta gcgatgccga aaagcccttt gataaaaaaa gaatcatcat cttaagaaat
      241 tcttagtcat ttattatgta aatgcttata aattcggccc tataatctga taaattatta
      301 agggcaaact tatgtgaaag ggtgataact atgagcgata aaatacttat tgtggatgat
      361 gaacatgaaa ttgccgattt ggttgaatta tacttaaaaa acgagaatta tacggttttc
      421 aaatactata ccgccaaaga agcattggaa tgtatagaca agtctgagat tgaccttgcc
      481 atattggaca tcatgcttcc cggcacaagc ggccttacta tctgtcaaaa aataagggac
      541 aagcacacct atccgattat catgctgacc gggaaagata cagaggtaga taaaattaca
      601 gggttaacaa tcggcgcgga tgattatata acgaagccct ttcgcccact ggagttaatt
      661 gctcgggtaa aggcccagtt gcgccgatac aaaaaattca gtggagtaaa ggagcagaac
      721 gaaaatgtta tcgtccactc cggccttgtc attaatgtta acacccatga gtgttatctg
      781 aacgagaagc agttatccct tactcccacc gagttttcaa tactgcgaat cctctgtgaa
      841 aacaagggga atgtggttag ctccgagctg ctatttcatg agatatgggg cgacgaatat
      901 ttcagcaaga gcaacaacac catcaccgtg catatccggc atttgcgcga aaaaatgaac
      961 gacaccattg ataatccgaa atatataaaa acggtatggg gggttggtta taaaattgaa
     1021 aaataaaaaa aacgactatt ccaaactaga acgaaaactt tacatgtata tcgttgcaat
     1081 tgttgtggta gcaattgtat tcgtgttgta tattcgttca atgatccgag ggaaacttgg
     1141 ggattggatc ttaagtattt tggaaaacaa atatgactta aatcacctgg acgcgatgaa
     1201 attatatcaa tattccatac ggaacaatat agatatcttt atttatgtgg cgattgtcat
     1261 tagtattctt attctatgtc gcgtcatgct ttcaaaattc gcaaaatact ttgacgagat
     1321 aaataccggc attgatgtac ttattcagaa cgaagataaa caaattgagc tttctgcgga
     1381 aatggatgtt atggaacaaa agctcaacac attaaaacgg actctggaaa agcgagagca
     1441 ggatgcaaag ctggccgaac aaagaaaaaa tgacgttgtt atgtacttgg cgcacgatat
     1501 taaaacgccc cttacatcca ttatcggtta tttgagcctg cttgacgagg ctccagacat
     1561 gccggtagat caaaaggcaa agtatgtgca tatcacgttg gacaaagcgt atcgactcga
     1621 acagctaatc gacgagtttt ttgagattac acggtataac ctacaaacga taacgctaac
     1681 aaaaacgcac atagacctat actatatgct ggtgcagatg accgatgaat tttatcctca
     1741 gctttccgca catggaaaac aggcggttat tcacgccccc gaggatctga ccgtgtccgg
     1801 cgaccctgat aaactcgcga gagtctttaa caacattttg aaaaacgccg ctgcatacag
     1861 tgaggataac agcatcattg acattaccgc gggcctctcc ggggatgtgg tgtcaatcga
     1921 attcaagaac actggaagca tcccaaaaga taagctagct gccatatttg aaaagttcta
     1981 taggctggac aatgctcgtt cttccgatac gggtggcgcg ggacttggat tggcgattgc
     2041 aaaagaaatt attgttcagc atggagggca gatttacgcg gaaagcaatg ataactatac
     2101 gacgtttagg gtagagcttc cagcgatgcc agacttggtt gataaaagga ggtcctaaga
     2161 gatgtatata attttggctc tttgtcaaat gatgttggta aagagaaatc t
//
LOCUS       DAIGOK010000322         1787 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.guided.223, whole genome shotgun sequence.
ACCESSION   DAIGOK010000322 DAIGOK010000000
VERSION     DAIGOK010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.guided.223"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            297..1337
                     /locus_tag="NOA01_002834"
     CDS             297..1337
                     /locus_tag="NOA01_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCI0633933.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            1435..1623
                     /locus_tag="NOA01_002835"
     CDS             1435..1623
                     /locus_tag="NOA01_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HCI0633934.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1681..>1787)
                     /locus_tag="NOA01_002836"
     CDS             complement(1681..>1787)
                     /locus_tag="NOA01_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HCI0633935.1"
                     /translation="RLQVEKYMDSFTDKELSLMEALAKGINEARNIEG"
BASE COUNT          598 a          277 c          319 g          593 t
ORIGIN      
        1 cattatcctg cccgttagtt gaataagcaa ttttttctat aagttatatt atcataatac
       61 cacaaaatca caaattttca ttcaattatc attttcaatt gcattttata aatcattttg
      121 tatgtaattt ggtatgtaag ttaaaatgaa aaaagtagtc cattttgaag tacattttgt
      181 cattttgtac tccaaaactg aactacttac ttgcatatat acgcctttat agtggagtac
      241 tacatttacg agattcggtt agagtatcag aaaaagaaaa aataaaagtt acagaaatgt
      301 atacttcaat aaatggtgaa ttatgtaaga ttgatagagc ttattctgga gaaattgtta
      361 ttttgcaaaa tgagtttttg aagttaaata gtgttcttgg agatacaaaa ctattgccac
      421 agagaaaaaa gattgaaaat ccgcaccctc tactacaaac aactgttgaa ccgagtaaac
      481 ctgaacagag agaaatgttg cttgatgccc ttttggaaat ctcagatagt gatccgcttc
      541 tacgatatta cgtggattct acgacacatg aaattatact ttctttctta gggaaagtac
      601 aaatggaagt gattagtgca ctgttgcaag aaaagtatca tgtggagata gaactaaaag
      661 agcctacagt catttatatg gagagaccgt taaaaaatgc agaatatacc attcacatcg
      721 aagtgccgcc aaatcctttc tgggcttcca ttggtttatc tgtatcaccg cttccgttgg
      781 gaagtggaat gcagtatgag agctcggttt ctcttggata cttaaatcaa tcatttcaaa
      841 atgcagttat ggaagggata cgctatggtt gcgaacaagg attatatggt tggaatgtga
      901 cggattgtaa aatctgtttt aagtacggtt tatactatag ccctgttagt actccagcag
      961 attttcggat gcttactcct attgtactgg agcaagcctt tagaaaagct ggaacagaat
     1021 tgttagagcc atatcttagt tttaaagttt atgcaccaca ggaatatctt tcacgggcat
     1081 ataacgatgc tcccaaatat tgtgcaaata tcgtaaatac tcaactgaaa aataatgagg
     1141 tcattattat tggagaaatt cctgctcgat gtattcaaga ttatcgcaat gatttaactt
     1201 tttttacaaa tgggcttagt gtttgtttag cagagctaaa aggatatcag gttaccactg
     1261 gcgaacctgt ttgccagacc cgtcgtctaa atagtcggat agataaagta agatatatgt
     1321 tcaataaaat aacttagtgc gttttatgtt gttatataaa tatggtttct tattaaataa
     1381 gatgaaatat tctttaatat agatttgaat taaagtggaa aggaggagat tgttattata
     1441 aactacaagt ggatattgtg tcctatttgt ggaaataaaa caagactacg aatacgagtg
     1501 gatactatac ttaaaaattt ccctttatac tgccccaaat gtaagaacga aactttaatt
     1561 aatgttcaaa aaatgaatat aataacaatc aaagagccag acgccaagac gcagagccga
     1621 taatttgaga aatgaaactc tcatcttatc ggctcttttt gtttatctga attttactga
     1681 ctagccttca atatttcttg cttcattgat accttttgct agtgcttcca ttaaggatag
     1741 ttctttgtct gtaaagctat ccatgtattt ttctacctgt aaccttc
//
LOCUS       DAIGOK010000323         1386 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.guided.237, whole genome shotgun sequence.
ACCESSION   DAIGOK010000323 DAIGOK010000000
VERSION     DAIGOK010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.guided.237"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            22..>1386
                     /gene="aph(2'')-I"
                     /locus_tag="NOA01_002837"
     CDS             22..>1386
                     /gene="aph(2'')-I"
                     /locus_tag="NOA01_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HCI0633936.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVY"
BASE COUNT          582 a          103 c          239 g          462 t
ORIGIN      
        1 tatgaaaaag gtgataaata aatgaatata gttgaaaatg aaatatgtat aagaacttta
       61 atagatgatg attttccttt gatgttaaaa tggttaactg atgaaagagt attagaattt
      121 tatgatggta gagataaaaa atatacatta gaatcattaa aaaaacatta tacagagcct
      181 tgggaagatg aagtttttag agtaattatt gaatataaca atgttcctat tggatatgga
      241 caaatatata aaatgtatga tgagttatat actgattatc attatccaaa aactgatgag
      301 atagtctatg gtatggatca atttatagga gagccaaatt attggagtaa aggaattggt
      361 acaagatata ttaaattgat ttttgaattt ttgaaaaaag aaagaaatgc taatgcagtt
      421 attttagacc ctcataaaaa taatccaaga gcaataaggg cataccaaaa atctggtttt
      481 agaattattg aagatttgcc agaacatgaa ttacacgagg gcaaaaaaga agattgttat
      541 ttaatggaat atagatatga tgataatgcc acaaatgtta aggcaatgaa atatttaatt
      601 gagcattact ttgataattt caaagtagat agtattgaaa taatcggtag tggttatgat
      661 agtgtggcat atttagttaa taatgaatac atttttaaaa caaaatttag tactaataag
      721 aaaaaaggtt atgcaaaaga aaaagcaata tataattttt taaatacaaa tttagaaact
      781 aatgtaaaaa ttcctaatat tgaatattcg tatattagtg atgaattatc tatactaggt
      841 tataaagaaa ttaaaggaac ttttttaaca ccagaaattt attctactat gtcagaagaa
      901 gaacaaaatt tgttaaaacg agatattgcc agttttttaa gacaaatgca cggtttagat
      961 tatacagata ttagtgaatg tactattgat aataaacaaa atgtattaga agagtatata
     1021 ttgttgcgtg aaactattta taatgattta actgatatag aaaaagatta tatagaaagt
     1081 tttatggaaa gactaaatgc aacaacagtt tttgagggta aaaagtgttt atgccataat
     1141 gattttagtt gtaatcatct attgttagat ggcaataata gattaactgg aataattgat
     1201 tttggagatt ctggaattat agatgaatat tgtgatttta tatacttact tgaagatagt
     1261 gaagaagaaa taggaacaaa ttttggagaa gatatattaa gaatgtatgg aaatatagat
     1321 attgagaaag caaaagaata tcaagatata gttgaagaat attatcctat tgaaactatt
     1381 gtttat
//
LOCUS       DAIGOK010000324         1386 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.guided.238, whole genome shotgun sequence.
ACCESSION   DAIGOK010000324 DAIGOK010000000
VERSION     DAIGOK010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.guided.238"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            22..>1386
                     /gene="aph(2'')-I"
                     /locus_tag="NOA01_002838"
     CDS             22..>1386
                     /gene="aph(2'')-I"
                     /locus_tag="NOA01_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HCI0633937.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYGGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVY"
BASE COUNT          581 a          103 c          240 g          462 t
ORIGIN      
        1 tatgaaaaag gtgataaata aatgaatata gttgaaaatg aaatatgtat aagaacttta
       61 atagatgatg attttccttt gatgttaaaa tggttaactg atgaaagagt attagaattt
      121 tatggtggta gagataaaaa atatacatta gaatcattaa aaaaacatta tacagagcct
      181 tgggaagatg aagtttttag agtaattatt gaatataaca atgttcctat tggatatgga
      241 caaatatata aaatgtatga tgagttatat actgattatc attatccaaa aactgatgag
      301 atagtctatg gtatggatca atttatagga gagccaaatt attggagtaa aggaattggt
      361 acaagatata ttaaattgat ttttgaattt ttgaaaaaag aaagaaatgc taatgcagtt
      421 attttagacc ctcataaaaa taatccaaga gcaataaggg cataccaaaa atctggtttt
      481 agaattattg aagatttgcc agaacatgaa ttacacgagg gcaaaaaaga agattgttat
      541 ttaatggaat atagatatga tgataatgcc acaaatgtta aggcaatgaa atatttaatt
      601 gagcattact ttgataattt caaagtagat agtattgaaa taatcggtag tggttatgat
      661 agtgtggcat atttagttaa taatgaatac atttttaaaa caaaatttag tactaataag
      721 aaaaaaggtt atgcaaaaga aaaagcaata tataattttt taaatacaaa tttagaaact
      781 aatgtaaaaa ttcctaatat tgaatattcg tatattagtg atgaattatc tatactaggt
      841 tataaagaaa ttaaaggaac ttttttaaca ccagaaattt attctactat gtcagaagaa
      901 gaacaaaatt tgttaaaacg agatattgcc agttttttaa gacaaatgca cggtttagat
      961 tatacagata ttagtgaatg tactattgat aataaacaaa atgtattaga agagtatata
     1021 ttgttgcgtg aaactattta taatgattta actgatatag aaaaagatta tatagaaagt
     1081 tttatggaaa gactaaatgc aacaacagtt tttgagggta aaaagtgttt atgccataat
     1141 gattttagtt gtaatcatct attgttagat ggcaataata gattaactgg aataattgat
     1201 tttggagatt ctggaattat agatgaatat tgtgatttta tatacttact tgaagatagt
     1261 gaagaagaaa taggaacaaa ttttggagaa gatatattaa gaatgtatgg aaatatagat
     1321 attgagaaag caaaagaata tcaagatata gttgaagaat attatcctat tgaaactatt
     1381 gtttat
//
LOCUS       DAIGOK010000325         1314 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.guided.240, whole genome shotgun sequence.
ACCESSION   DAIGOK010000325 DAIGOK010000000
VERSION     DAIGOK010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1314
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.guided.240"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            <1..>1314
                     /locus_tag="NOA01_002839"
                     /note="aph(2'')-Ia"
     CDS             <1..>1314
                     /locus_tag="NOA01_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HCI0633938.1"
                     /translation="ESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYH
                     YPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAI
                     RAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVD
                     SIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIE
                     YSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISE
                     CTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSC
                     NHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIE
                     KAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRVKSRLLCKMLYN"
BASE COUNT          548 a          108 c          226 g          432 t
ORIGIN      
        1 gaatcattaa aaaaacatta tacagagcct tgggaagatg aagtttttag agtaattatt
       61 gaatataaca atgttcctat tggatatgga caaatatata aaatgtatga tgagttatat
      121 actgattatc attatccaaa aactgatgag atagtctatg gtatggatca atttatagga
      181 gagccaaatt attggagtaa aggaattggt acaagatata ttaaattgat ttttgaattt
      241 ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa taatccaaga
      301 gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc agaacatgaa
      361 ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga tgataatgcc
      421 acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt caaagtagat
      481 agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa taatgaatac
      541 atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga aaaagcaata
      601 tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat tgaatattcg
      661 tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac ttttttaaca
      721 ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg agatattgcc
      781 agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg tactattgat
      841 aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta taatgattta
      901 actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc aacaacagtt
      961 tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct attgttagat
     1021 ggcaataata gattaactgg aataattgat tttggagatt ctggaattat agatgaatat
     1081 tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa ttttggagaa
     1141 gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata tcaagatata
     1201 gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat taaacaggaa
     1261 tttatcgaaa atggtagagt caagtccaga ctcctgtgta aaatgctata caat
//
LOCUS       DAIGOK010000326          944 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.guided.253, whole genome shotgun sequence.
ACCESSION   DAIGOK010000326 DAIGOK010000000
VERSION     DAIGOK010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.guided.253"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            229..777
                     /gene="aac(6')"
                     /locus_tag="NOA01_002840"
     CDS             229..777
                     /gene="aac(6')"
                     /locus_tag="NOA01_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HCI0633939.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          333 a          141 c          201 g          269 t
ORIGIN      
        1 aaagataatc tatatcatga tgaacaaaca gagttttttc ctccaattga tggctttcct
       61 gatctttcct attttgaata aagtgtttct gtataatttt tttattttat aagtaagata
      121 aataatgaaa gaatagagat actgattggt agctaaagat ttttgagcga gaacatttcg
      181 ttttttcaaa aggactgtta gaataagcaa gaacaagggg gaagacgtat gataatcagt
      241 gaatttgacc gtaataatcc agtattgaaa gatcagcttt ctgatttact gagactgact
      301 tggccggaag aatatggaga cagctcggca gaagaagtag aagaaatgat gaatccagaa
      361 cgaatcgcgg tagcagcggt agaccaagat gagttagtag gatttattgg tgcaatccct
      421 caatacggta tcacaggttg ggaattgcat ccattagttg tagaaagctc ccgacgaaag
      481 aaccaaatag gtactcgatt agtcaattac ttagaaaaag aagtagcttc cagaggagga
      541 atcacgattt atttaggtac ggatgattta gaccatggaa caacgttaag tcaaacggac
      601 ctgtatgagc atacatttga taaagtggct tccatccaga accttcgtga acatccgtat
      661 gaattctatg aaaaattagg ttataaaatc gtaggtgtct taccaaatgc aaatggctgg
      721 gacaaaccgg atatttggat ggcaaaaacg attattcctc gaccaaattc tcaataataa
      781 aaagaggttg tgacagaagt aatcagcttc aagaaataag aaggaattta cgaaaattac
      841 ttcttgaatt tttgtgaaat tccaacttat ttccgaagaa gctacttttg gaacaccgtg
      901 taccagaggt tgtgaaaaag tcgcttagtt tcgagtagta agaa
//
LOCUS       DAIGOK010000327          877 bp    DNA     linear   BCT 18-JUL-2022
DEFINITION  TPA_asm: Enterococcus faecium strain microbial isolate A1200-ENTFAE
            SAMN29456561-rid14856313.guided.259, whole genome shotgun sequence.
ACCESSION   DAIGOK010000327 DAIGOK010000000
VERSION     DAIGOK010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN29456561
            Sequence Read Archive: SRR19916929
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (15-JUL-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 15-JUL-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001361204.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001361204.1
            Genome Coverage       :: 101x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 07/16/2022 04:01:21
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,842
            CDSs (total)                      :: 2,781
            Genes (coding)                    :: 2,700
            CDSs (with protein)               :: 2,700
            Genes (RNA)                       :: 61
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 53
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 81
            CDSs (without protein)            :: 81
            Pseudo Genes (ambiguous residues) :: 0 of 81
            Pseudo Genes (frameshifted)       :: 33 of 81
            Pseudo Genes (incomplete)         :: 34 of 81
            Pseudo Genes (internal stop)      :: 26 of 81
            Pseudo Genes (multiple problems)  :: 10 of 81
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN29456561-rid14856313.guided.259"
                     /strain="microbial"
                     /isolate="A1200-ENTFAE"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /lat_lon="52.52437 N 13.41053 E"
                     /collection_date="2019-11-25"
     gene            103..600
                     /gene="dfrG"
                     /locus_tag="NOA01_002841"
     CDS             103..600
                     /gene="dfrG"
                     /locus_tag="NOA01_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HCI0633940.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(672..>877)
                     /locus_tag="NOA01_002842"
     CDS             complement(672..>877)
                     /locus_tag="NOA01_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HCI0633941.1"
                     /translation="TTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSADKKV
                     LTPAQRLGITDKQFDWKDIIYFK"
BASE COUNT          319 a          106 c          179 g          273 t
ORIGIN      
        1 gaaaaaattg cttattcaac taacgggcag gataatgaga aaaggttttt tgatagaaat
       61 aagaaattag atggtaaatt agttaaaaag gctgtgttat aaatgaaagt ttctttgatt
      121 gctgcgatgg ataagaatag agtgattggc aaagagaatg acattccttg gaggattccc
      181 aaggactggg aatatgttaa aaatactaca aagggacatc cgataatatt aggtaggaag
      241 aaccttgaat caatcggaag agccttacct gacagaagaa atattattct gacgagagat
      301 aaggggttta cctttaatgg ttgtgaaatt gttcattcaa tagaagatgt ttttgagtta
      361 tgtaaaaacg aagaagaaat ttttattttc ggaggagaac agatttataa tttgtttttc
      421 ccttatgttg agaaaatgta catcacaaaa atacatcatg aattcgaagg agatactttt
      481 tttccagaag tgaattatga ggaatggaat gaggtatttg cccaaaaagg gataaagaat
      541 gataaaaatc cgtataacta ctattttcat gtatatgaaa gaaaaaactt attgagttaa
      601 cgggtgcgtt aattgaagaa gaacagtaaa gataaagcac aagagcttgg gattactcca
      661 agctcttttt gtcatttgaa ataaattata tctttccaat caaattgttt atctgtaatc
      721 cctaatcttt gagcaggtgt caaaaccttt ttatccgctg atttaaaagg gcgacaaaag
      781 ttgtagtaag ttcttagaat tgtaatcgca aattgagcat acttcggatt gaagttagcg
      841 taaatatagc tcttcttatc accacgagct gttgtta
//
